LOCUS       DAOQWE010000001        23309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_2548_length_23309_cov_6.874301, whole genome shotgun sequence.
ACCESSION   DAOQWE010000001 DAOQWE010000000
VERSION     DAOQWE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 23309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23309
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2548_length_23309_cov_6.874301"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..508)
                     /locus_tag="PKK37_00005"
     CDS             complement(317..508)
                     /locus_tag="PKK37_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HNR80800.1"
                     /translation="MSRICQLCEKKGMMAWKRVRLRATKFNPTAKRKQQPNLQSATLP
                     DGTKIVACAKCLKTLHKAK"
     gene            733..1362
                     /locus_tag="PKK37_00010"
     CDS             733..1362
                     /locus_tag="PKK37_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR80801.1"
                     /translation="MELISTAFLIIALVFSVVIHEVAHGSAAAAQGDFTAKYAGRLTL
                     NPLKHLDWVGSVLVPGFLIVAGAPILLGWAKPVPVNPLNFRDKRWGDAKVSIAGAAAN
                     LGLALVFGLALRFWPEVSGAAAANFYALAQSVVIINLVLAVFNMVPVPPLDGSHVLFA
                     LLPASQNEFKVFLQKYGFVFLLVFLLFFVPVIWPIVAFLYQLITGIPLI"
     gene            complement(1394..1519)
                     /locus_tag="PKK37_00015"
     CDS             complement(1394..1519)
                     /locus_tag="PKK37_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80802.1"
                     /translation="MKIEKVKTKTFLETVFLAQTKYYYHKKKQKSNSGGSSVFQQ"
     gene            1534..1944
                     /gene="rpsL"
                     /locus_tag="PKK37_00020"
     CDS             1534..1944
                     /gene="rpsL"
                     /locus_tag="PKK37_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNR80803.1"
                     /translation="MATIHQLIKSKRTKRAHKTKSPALSFGFNALKNRPRDYVSPFKR
                     GICTKVFTATPKKPNSALRKVARVKLTNGMEVTAYIPGEGHKLQEHSIVLIRGGRVKD
                     LPGVRYHVVRGKLDCAGVDKRKQERSKYGAKKPK"
     gene            2064..2531
                     /gene="rpsG"
                     /locus_tag="PKK37_00025"
     CDS             2064..2531
                     /gene="rpsG"
                     /locus_tag="PKK37_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012307671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNR80804.1"
                     /translation="MPRGKITKREVAPDMVYNSVAIAQIINHIMKRGKKNTAKKIVYG
                     ALEIIKEKTGKEPLEVFESAMENARPLLEVKSQRVGGATYQVPKPVAKDRGTALAVRW
                     LLGTARKKKGQPMKVKLAEELIGASNNTGAAIKKKEDTHRMAEANRAFAHFRF"
     gene            2659..>4152
                     /gene="fusA"
                     /locus_tag="PKK37_00030"
     CDS             2659..>4152
                     /gene="fusA"
                     /locus_tag="PKK37_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR80805.1"
                     /translation="MARQAPLEKYRNFGVIAHIDAGKTTTSERILFYTGLSHKIGEVH
                     EGAAIMDWMAQERERGITITSAATTCFWSPGEYLSKQKTDQHQFNIIDTPGHIDFTAE
                     VQRALRVLDGAVVVFDGVAGVQPQSETVWRQADKYAVPRICFINKMDRIGASFERSLQ
                     SIWKKLNPNAVAANIPIGEEDKLSGVVDLIKMKALRFTGANGQEVVEEEIPADMAEMA
                     SAWRAKLVEKIVSEDEDLMNKYLAGEEIPSDDLRRVLRSATIKGNIIPVFYGSSLKNT
                     GVQPVLDAVCYYLPSPLDLPPMKGMEVRGEGEVLRHPDDNEPFTALAFKVAADPYVGT
                     LTFFRVYSGVLQKGSYVLNTATGEKERIGRLLRMYSNDRKEIDEVRAGDIAATVGLKN
                     TSTGHTLCDESHPIILEKISFPEPVIDIRIEPKTKADQEKMGFALKKLSDEDPTFRVK
                     TDQETGEVIIGGMGELHLDILVDRMKREFKVEANVGRPQVAYKETVKT"
     assembly_gap    4153..4252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4253..4627
                     /gene="fusA"
                     /locus_tag="PKK37_00035"
     CDS             <4253..4627
                     /gene="fusA"
                     /locus_tag="PKK37_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106397.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR80806.1"
                     /translation="GSFHEVDSSEMAFKIAGSMALQAAARKANPVLLEPIMKLEVTIP
                     QEFFGDVIGDLSSRRGRIEETQDQMESKIIDVKVPLSEMFGYATNLRSLTQGRGTFTM
                     EFDHYEEVPANVAQEIVSGKRK"
     gene            4706..5905
                     /gene="tuf"
                     /locus_tag="PKK37_00040"
     CDS             4706..5905
                     /gene="tuf"
                     /locus_tag="PKK37_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006655954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNR80807.1"
                     /translation="MAEKEKFVRGKSHLNIGTIGHVDHGKTTLSAAILHSLGLKGNKV
                     AMKSIDQIDSAPEEKQRGVTINISHLEYETEKRHYAHIDCPGHADYIKNMITGAAQMD
                     GGILVVSAADGAMPQTREHILLAHQVGLPAMVVFLNKCDQVDDPEMIDLVESEVRELL
                     KKYHFPGDEIPFVRGSALKALEGKSADDEAVKAVIELMNKVDEYIPEPVRDTEKPFLM
                     AIEDVFSIAGRGTVATGRIERGVVKSNEEVEIVGIKPTAKTTAVSIEMFQKILDEGRA
                     GDNVGILLRGTKKEDIERGQVLAKPGSITPHTEFTSEVYILTKEEGGRHTPFFSGYKP
                     QFYVRTCDVTGEVTLNPGVEMVMPGDTVTFNVKLINPIALEEKSKFAIREGGKTVGAG
                     VVTKIVK"
     gene            5924..6274
                     /gene="rpsJ"
                     /locus_tag="PKK37_00045"
     CDS             5924..6274
                     /gene="rpsJ"
                     /locus_tag="PKK37_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNR80808.1"
                     /translation="MATATIKKNKETKEAARPKLRIKLRAYDHKVIDNCTKQIIETIN
                     RYGAEILGPIPLPIEIHKYTVNRSTFVHKDAREQFEIRVHKRLLDIMNPTPKIIDSLR
                     NLTLPAGVDIEIKM"
     gene            6365..6964
                     /gene="rplC"
                     /locus_tag="PKK37_00050"
     CDS             6365..6964
                     /gene="rplC"
                     /locus_tag="PKK37_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNR80809.1"
                     /translation="MMKFILGKKVGMTEMFAPDGKKIPVTLVCAGPCTVTQIKTAERD
                     GYRAAQIGIEEIAAAKVAKSRQKKPFRFLREFQIDAVAELKAGDVVSVANFQEGDKVK
                     VAGISKGKGFQGAVKRHGFKGQQTCTHGTKHELRNLGSVGMGEASIRKGRKMPGRMGA
                     ERITVGNLKIVKIDPENNLLALRGAVPGNKGTLLEIRGS"
     gene            7025..7753
                     /gene="rplD"
                     /locus_tag="PKK37_00055"
     CDS             7025..7753
                     /gene="rplD"
                     /locus_tag="PKK37_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNR80810.1"
                     /translation="MEAIVYNQKGEKLNAVNLPEGVFGVKMNPDLVYQVAITQAANRR
                     RITAHTKDRGEVSGGGKKPWRQKGTGRSRHGSNRSPIWRHGGVVFGPRNDKVFGGKIN
                     KKMRRKALAMVLSAKASGGMMVLVDKIEFDVPKTKVMAQMLEAIKKVNENFKTGKVLV
                     ALPEFEKNAVLSGRNIPGAEMIEAAKLNTLDLLNAKCLLLPSAAVKVVERIVAGGENE
                     KSEKKSEKQEKSERKPSAKKTTKK"
     gene            7790..8191
                     /gene="rplW"
                     /locus_tag="PKK37_00060"
     CDS             7790..8191
                     /gene="rplW"
                     /locus_tag="PKK37_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNR80811.1"
                     /translation="MAITDIFKKEEAQKGKKKPGAKPAAEKTDKKEARQPKNAGRAMR
                     VLAGVHATEKAANMAEEERYVFKVAKTAGKKEIARAVEDYYGVDVVSVNVINIPGRNR
                     RNRRGIYFESGYRKAAVKIKKGQTIEAAATK"
     gene            8229..>8974
                     /gene="rplB"
                     /locus_tag="PKK37_00065"
     CDS             8229..>8974
                     /gene="rplB"
                     /locus_tag="PKK37_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNR80812.1"
                     /translation="MKSILSKKRPEKHLLKSLKSNAGRNFSGRITVRHRGGGVKRMYR
                     MVDFGQEKLGIEGGIVALEYDPNRTAYLALVEYRDGTRRYVLACDQSLAGDKIVCEEN
                     AEIKTGNRLMLKNIPVGTEVCNIELRPGKGGQLARSAGSSAKLMAHEGKFAQLQMPSK
                     EIRRVPAECFATIGKISHPEHRFEDYGRAGAKRRKGWRPTVRGSVMNPCDHPHGGGEG
                     RTGIGMKRAKTPWGKPAMGVRTRNRNKWTS"
     assembly_gap    8975..9074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9192..9482
                     /gene="rpsS"
                     /locus_tag="PKK37_00070"
     CDS             9192..9482
                     /gene="rpsS"
                     /locus_tag="PKK37_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNR80813.1"
                     /translation="MARSIKKGPFVDEKLMEKVKKGGGAVIKTWSRRSTIVPEMIGVV
                     FAVHNGKEFITVRPNEDMVGHKLGEFSPTTKFHRHGGKMQKDLETKQAPVKK"
     gene            9504..9857
                     /gene="rplV"
                     /locus_tag="PKK37_00075"
     CDS             9504..9857
                     /gene="rplV"
                     /locus_tag="PKK37_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNR80814.1"
                     /translation="MAITVKLRQLKIAERKTRMVADLVRYKTVEEAEAILSFTPKKAS
                     LPLLKLLRSAAASAKNTYKFDPKNLYIAKLTVDGGPMQKRVFPRSRGRADHIEKRTSH
                     ITLVLEQKLEIENKK"
     gene            9899..10555
                     /gene="rpsC"
                     /locus_tag="PKK37_00080"
     CDS             9899..10555
                     /gene="rpsC"
                     /locus_tag="PKK37_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNR80815.1"
                     /translation="MAHKVHPKAFRIKDASDWYSRGYYGEKFPKLLEEDFKIREFLHR
                     RLAGIGISEVEIERFPGRINVLVSCVRPGLIIGRQGQGIEELRQKLQMLVFGGNAKKK
                     GGADLKVEVREIASPWASAAYIGQSVASQIEKRMPYLKVTKQSIEKAMTSREVEGVRI
                     QLSGRLNGVEIARTAWLAKGRLPRQTIRADIDYAITEAHCTYGVIGIKVWVYKGEKQK
                     "
     gene            10654..11076
                     /gene="rplP"
                     /locus_tag="PKK37_00085"
     CDS             10654..11076
                     /gene="rplP"
                     /locus_tag="PKK37_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNR80816.1"
                     /translation="MSLLMPKKIKHRKWQKGRRRGLGVALRGAELNYGSFGLKAMEEK
                     WITSRQIEAGRRAILRYLKKGGKLWIRIFPDKPVTSKGEEVPLGGGKGAVDHYAFVVK
                     PGRIMYEIDGIKEEMARQALEKAGDKLPIKAKFIRKEA"
     gene            11096..11296
                     /gene="rpmC"
                     /locus_tag="PKK37_00090"
     CDS             11096..11296
                     /gene="rpmC"
                     /locus_tag="PKK37_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNR80817.1"
                     /translation="MKAAELRQKNIAELEGLLEEKRARIARLRFDLEGGKTKNLKEYR
                     QIKLDIARVKTLLSEGMSADKN"
     gene            11342..11626
                     /gene="rpsQ"
                     /locus_tag="PKK37_00095"
     CDS             11342..11626
                     /gene="rpsQ"
                     /locus_tag="PKK37_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNR80818.1"
                     /translation="MKHQLTGKVVSDKMQKTVVVMTESLKEHPKYHKRYRVSKKYKAH
                     DENNKCKIGDVVLIEECRPLSADKRWKVAKKIAAGREEEAVKAAGAKEEE"
     gene            11709..12080
                     /gene="rplN"
                     /locus_tag="PKK37_00100"
     CDS             11709..12080
                     /gene="rplN"
                     /locus_tag="PKK37_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010881646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNR80819.1"
                     /translation="MIQLRTMLKVADNSGAKMVQCFHVAGGTRRRYAAVGDVIMAAVK
                     IAEPRKPVKKHDKVRAVVIRQKKEYRREDGTYIRFDDNACVILEGKGKEPKGGRIFGP
                     VAKELKARGYDKIAAMAEELI"
     gene            12180..12503
                     /gene="rplX"
                     /locus_tag="PKK37_00105"
     CDS             12180..12503
                     /gene="rplX"
                     /locus_tag="PKK37_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNR80820.1"
                     /translation="MKIIKGDNVLVISGKDRLKTGKVLRSLPAVGRLVVEGVNVAKKS
                     RRPRKQGEKGQIVSLPMPIDASNVKLLCPKCGKPARVAYKAAEGRNPKEKNKTRICKK
                     CSAEI"
     gene            12532..13092
                     /gene="rplE"
                     /locus_tag="PKK37_00110"
     CDS             12532..13092
                     /gene="rplE"
                     /locus_tag="PKK37_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNR80821.1"
                     /translation="MAGLYEKYKKETVPALVKQFGWRNENQAPRLEKATVNCGFGRMV
                     SGKTKDEQKKIQEAIARDLTQICGQRAVICAARKSIAGFKIREGQSVGAKITLRRRRM
                     YDFLERAITVALPRTRDFQGIPSKNVDRDGNLTFGIKEHIAFPEVSPEKINFIFSFEV
                     TVTTTARNREEALAMFKSLGFPIKKD"
     gene            13147..13332
                     /locus_tag="PKK37_00115"
     CDS             13147..13332
                     /locus_tag="PKK37_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNR80822.1"
                     /translation="MATKAQIAKSKRTPKFKSRVVRRCFRCGRKHGYMRKFGLCRICF
                     REAANQGLIPGVAKSSW"
     gene            13456..13860
                     /gene="rpsH"
                     /locus_tag="PKK37_00120"
     CDS             13456..13860
                     /gene="rpsH"
                     /locus_tag="PKK37_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNR80823.1"
                     /translation="MDKVADMLTVIRNAQAAGKPAVAVPYSALKYNIAKILERRGYVA
                     TVEKRSRKAKKNGKARPCLEISLKYVDSVPAITGCEKVSRPGQRIYVPASDIKRVKQG
                     KGIGIISTSKGLMTEYDAKKQNIGGEILCQIW"
     gene            13886..14431
                     /gene="rplF"
                     /locus_tag="PKK37_00125"
     CDS             13886..14431
                     /gene="rplF"
                     /locus_tag="PKK37_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNR80824.1"
                     /translation="MSRIGKKPVNIPAGVTVEVSGDEVKIKGAKGELSRRFGSKVAIV
                     REGDALKVSPAEGSKAARAMWGTVRMHLANMVQGVCAGFEKQLQIEGIGYRAAVEGRD
                     LVLNIGFTHPVRIAAPAGIAFKVEKNIVTVSGCDKETVGKIAAQVRRVRPPEPYKGKG
                     IRYVGENVRRKAGKKAAGASK"
     gene            14458..14844
                     /gene="rplR"
                     /locus_tag="PKK37_00130"
     CDS             14458..14844
                     /gene="rplR"
                     /locus_tag="PKK37_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNR80825.1"
                     /translation="MSISNKTRHKFVRRANRHRRVRARIAGTGETPRLCVFRSNRHIF
                     AQLIDDRRGKTLAACGDGDVSAEQTAEKSDLSGKCASAFAVGRRIARLAGELKISRAV
                     FDRGGYRYHGRVKSLAEGARGGGLKF"
     gene            14879..15625
                     /locus_tag="PKK37_00135"
     CDS             14879..15625
                     /locus_tag="PKK37_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNR80826.1"
                     /translation="MEASQTTPVKSEAAKPAFKPFPSAGARPQFNRNRPGQGGGGSSR
                     FGAGRFGGRGRQGGGRRDDGGRKDEFDSKLLDLSRVSHTRAGGRRLRFRAIVISGNKA
                     GKVGLGVASGLDVAQAVEKATVQSKKKMIVVPTAGGTISREVAAKVGPARVIIKPQRK
                     GRGLMAGGVVRLLCQLAGIKDISSKMLGTTKNKLNNARAVMRAFEDLSRQSRKDSAQN
                     PEPGAQNKIEAEKENPKNSPAADGAAKVSQ"
     gene            15639..16106
                     /locus_tag="PKK37_00140"
     CDS             15639..16106
                     /locus_tag="PKK37_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HNR80827.1"
                     /translation="MQIHQLKNSSGAKTRKRVGRGGKRGTYSGRGGKGQTARAGRNFR
                     PIIREVLKRYPKLRGHRNRAAGLTAFAVNLAVLEAGFENGAAIDPRVLIEKNIVRCAG
                     GKTPRVKILGRGEISKKFNISGCEISAAAKAKIEKAGGVVNQAVLPPSGEAFS"
     gene            16118..17404
                     /gene="secY"
                     /locus_tag="PKK37_00145"
     CDS             16118..17404
                     /gene="secY"
                     /locus_tag="PKK37_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNR80828.1"
                     /translation="MWYQAILQPFRVKELRNKIFFVLGAFAVFRLMANIPITGVDTNQ
                     LKQFFGEFGIFQLLGAFTGGSLDRFSVAMLGLGPYITAIIIFQLMTMIFPALERLYKE
                     EGEAGRAKFNQYARIATVPLALLQGFAMIALLSGPNVGILHFDSLLEKIAALFSITAG
                     ALLLMWIGELMTEKGIGNGVSLLIFAGIMADFPRNIRQMIEVYDSSMALGYIAFFVMA
                     AAMVAAVVLVNEARRNIPVSYAKRMRGTKMTGGVSTYLPLNINPAGVIPIIFALSILI
                     FPPMVAQFFASSGGWVGDAARAVEGLFAMDGAFYMISYFVLVVLFTYFYTAVTFDPKQ
                     ISKNLQKMGGFIPGIRPGQPTADYLRYILNRVLLLGAVFLGLIAVMPQIVGPFTGVTS
                     FSFLIGGTSLLIAVSVILETARQIKAQLQMREYENF"
     gene            17391..18149
                     /locus_tag="PKK37_00150"
     CDS             17391..18149
                     /locus_tag="PKK37_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNR80829.1"
                     /translation="MKIFNRAILKNLNGNQIQKAILLFGPPGAGKGTQAELLASKFYF
                     YNLDTSRVLEEKFKNAKPGAQVKIHNAVYPVADELAKRKKGVLCSVEFTAFLMNKRIA
                     EIHGLGKSLILSGSPRSLAEAEIEMPFLEELYGKENIHILFLDLPAEQTIFRNSNRRI
                     CELMRHSILYIPENANLKRCPLDGSRLMHRDLDNVETIKVRLEEFEQTAAPLEDYYRR
                     HEYNLKKINADQYVENVFDDIIAALGPGFKVYEM"
     gene            18185..18958
                     /gene="map"
                     /locus_tag="PKK37_00155"
     CDS             18185..18958
                     /gene="map"
                     /locus_tag="PKK37_00155"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNR80830.1"
                     /translation="MIKIKSPEEIGIMAEGGRLLAAVLEELKKKTAAGASTKELDDLA
                     VSLIAAAGGRPSFLGEGGYPATICVSINDEVVHGVPSPKRIIKNGDIVSLDAGMIHRG
                     FHTDMAVTVAVGEVDPELRRMIKAAKKALKYAIKKARPGNTFGDVGNTVERYVESQGF
                     HVVRDLCGHGIGKKLHEDPQILNYGRRHRGPVIKEGMVFCVEPMITAGNPEIILASDK
                     QAYKIKDGSPAAHWEHTLAIVNGACRVLTAFPDTEDKED"
     gene            19039..20067
                     /gene="galT"
                     /locus_tag="PKK37_00160"
     CDS             19039..20067
                     /gene="galT"
                     /locus_tag="PKK37_00160"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNR80831.1"
                     /translation="MKKNQCCPSQLRYDVVSKDWVVIAPGRGKRPEEYKRQRKIIEMD
                     LRQCPFCNLTEAADPLLVFSAGKIAAKKTLPGDWTLAVIPNKFPAFYPITGKSVCQWK
                     IGPVYRSMDAVGFCEVVVTRDHDCHLGLMDLPATEEVMAAYQTRYLELKEHKLVKYIS
                     IFHNHGAEAGASQPHPHSQIITSPLIDVDLLGELENSKKYWRTHKKCLACEMNKFELK
                     DRSRVVCENADFVASCPFASKTAFKVVISPKFHSPRFENIDPGQRKSLTQILSAAIKK
                     IYNALGDPAYNFYLHTAPCDRKDYSHYHWHLTIVPKTAIMAGFEIGTRIEISTIEPER
                     AAEYLRAQ"
     gene            20143..20388
                     /locus_tag="PKK37_00165"
     CDS             20143..20388
                     /locus_tag="PKK37_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR80832.1"
                     /translation="MKTYTFRVIIEPDGKAFHGYVPILRGCHTCGKTLEETKKNLKEA
                     IKCHVQGLVRDGENIPQEENAFELVQTFTDKELALTC"
     gene            20392..20616
                     /locus_tag="PKK37_00170"
     CDS             20392..20616
                     /locus_tag="PKK37_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR80833.1"
                     /translation="MAKLPAVKAQKLIKVLLKLGFFKYSQVGSHAQFKHPGGRRTTVP
                     IHQGKEISPGTLLAILKDIEVSKDDFIKLL"
     gene            20779..21504
                     /gene="tpiA"
                     /locus_tag="PKK37_00175"
     CDS             20779..21504
                     /gene="tpiA"
                     /locus_tag="PKK37_00175"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNR80834.1"
                     /translation="MKYIVGNWKCHPANKIEAKKILLGYKKGVKNSAKAKAVICPPAC
                     YLELAKKILKNSAELGGQNCHWEEGAFTGEISATQLESAGCKYVILGHSERRHKFGET
                     DQAINKKIIAALTAGLVPIFCCGETREQRIDGQITDIVETQIQEGLKGIDLNSAPVMI
                     AYEPVWAIGTGQACDSEEAAVISRFIKNITNEKIPLLYGGSVNSKNAASYIKNADYDG
                     LLAGGASLDPKEFSAIFTTLHSL"
     gene            21613..21834
                     /locus_tag="PKK37_00180"
     CDS             21613..21834
                     /locus_tag="PKK37_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR80835.1"
                     /translation="MIYKFTTIIIREGKWFVARCAELGVVSQGKTVLEAKKNIKEAVE
                     LYLEDNPKSKKQLSSGGAPLIARLELEYA"
     gene            21827..22069
                     /locus_tag="PKK37_00185"
     CDS             21827..22069
                     /locus_tag="PKK37_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013466399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR80836.1"
                     /translation="MPKKVFSGKETIRILCREFGFYFVSQKGSHVKLGKMSGKREIIT
                     VVPLHKELAIGTFRGVLELAEIELKEFLSKSDRSFR"
     gene            22473..23018
                     /locus_tag="PKK37_00190"
     CDS             22473..23018
                     /locus_tag="PKK37_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80837.1"
                     /translation="MNYQNSKGVVSIIILAAVAIAGVVLVDQFSRINERISRLEQANP
                     AAALLSPAETANGDSIGAQGIAGETKAEEEPVGEVIVPDDSVTISGKVVKVEKDAVTI
                     TMMPGGEDYDFVALVNENTFVNKQKDAADQKASPAEISEIKIGDFAVIESAGQIGDDK
                     KFTARKINFFEMPEEQPQSQQ"
BASE COUNT         6322 a         5162 c         6346 g         5279 t
ORIGIN      
        1 cccactttca tttcgggatt actttttcct cgcagggtta tcaaaaaaat ccttatcaat
       61 gaaggatttt tttgatgccg cctctatact atattatggt cggggaaggc agaatcgaac
      121 tgccgctaca cccaccccaa gggtgcggac taccactatc cgattccccg gactcgggac
      181 gctttccgaa ataactgtcg cgccatacgg gtagtattac atggaggcaa ttctgaaaaa
      241 cgtccttaac caacaaaccc gttttgcacg ggtttgtatc gcgaaggaaa tatctcgttc
      301 ctcggcgcgc ggcagtttat ttcgctttgt gcagggtttt gaggcatttg gcgcaggcga
      361 caattttggt gccgtcgggc aaagtggccg actgcagatt cggttgttgt tttcgcttgg
      421 cggtggggtt gaatttggtg gcgcgcagac gcaccctttt ccatgccatc attccttttt
      481 tttcgcaaag ctggcagatt ctggacataa ttttcttgaa atccgaatat tcgaatgctc
      541 gaaatccgaa acgttaacgc tgacgaacca atcataagat attttccgac gattggcaag
      601 aggcgggata aatgctagaa tacaaaaatc aaaattgaga ttggaaaagt aaaaacgaaa
      661 attaaaaatt aaaaattatc aatacggatt gtttcattct taatttttag attttgattt
      721 ttaatttgga atatggaatt gatttcaacc gcgtttttaa ttatcgcatt ggtgttttcg
      781 gtggtgatcc acgaagtggc gcacggatcg gcggcggcgg cgcagggaga tttcaccgcc
      841 aaatacgcgg gccggctgac gctcaatccg ctcaagcatc ttgactgggt cgggtcggtg
      901 ctggtgcccg gatttttgat cgttgccggc gcgccgatac ttctgggatg ggccaagccc
      961 gtgccggtca atccgctgaa ttttcgcgac aagagatggg gagacgccaa agtttccatc
     1021 gccggtgccg cggcgaatct gggtttggcg ctggttttcg gccttgcctt gcggttttgg
     1081 ccggaagttt ccggcgccgc ggccgccaat ttttacgctt tggcgcagtc ggtggtgatc
     1141 atcaatctgg tgctggcggt tttcaacatg gttcccgtgc cgccgctgga cggttcgcat
     1201 gttttgttcg cgcttttgcc ggcatcgcaa aacgaattca aagttttcct gcaaaaatac
     1261 gggtttgttt ttttgctggt ctttttgctg tttttcgttc ccgtcatctg gccgatcgtc
     1321 gcgtttttat accagctgat caccggcatc ccgctgatct agtgcggcaa tcgcgtttgc
     1381 gccgcattaa cagtcattgc tgaaacaccg atgaaccgcc gctgtttgac ttttgttttt
     1441 ttttgtgata gtaatatttt gtttgggcta aaaaaacagt ctcaagaaag gtttttgttt
     1501 ttactttttc aattttcatt tttaatttga aacatggcaa cgattcacca gcttatcaag
     1561 agcaagcgca ccaagcgcgc gcacaagacc aagtcgccgg cgctttcttt cgggtttaac
     1621 gccctgaaaa accgcccgcg tgattacgtt tcgccgttca agcggggcat ttgcaccaag
     1681 gtgttcacgg cgactcccaa aaaaccgaat tcggcgttgc gcaaagtggc gcgggtgaag
     1741 ctcaccaacg gcatggaagt gacggcctat attcccggag agggccacaa attgcaggag
     1801 cactccatcg tgttgatccg cggcggccgg gtaaaggatc tgccgggcgt gcgctatcac
     1861 gtggtgcgcg gcaagctgga ttgcgcgggc gtggacaaac gcaaacagga aagatcaaaa
     1921 tacggtgcca agaaacccaa gtaaataaaa tagattaaaa aatcaaaaat caaaatcaag
     1981 gaataaaata tcacgggaat tttttgcaaa aaattcccgc tccgaaattt ttcatttttc
     2041 aattttaatt ttaacttttt aagatgcctc gaggaaaaat caccaaaaga gaagtcgctc
     2101 cggatatggt ctacaacagc gtggcgatcg cgcagatcat caatcacatt atgaagcggg
     2161 ggaaaaagaa caccgccaaa aagattgtct acggcgcgct ggaaatcatt aaggaaaaaa
     2221 ccggcaagga accgctggaa gtttttgagt cggccatgga gaatgcgcgg cctctattgg
     2281 aggtcaagtc ccagcgcgtc ggcggcgcga cctatcaggt tcccaagccg gtggccaaag
     2341 atcggggcac ggcgctggcg gtgcgctggc ttttgggcac ggcgcgcaag aaaaagggcc
     2401 agccgatgaa agtgaaactg gccgaggagc tgattggcgc gtccaacaat acgggggcgg
     2461 ccatcaagaa aaaagaagac acgcaccgca tggccgaggc caaccgggcg tttgcacatt
     2521 tccgtttcta aaatataaaa ttccaataac caagcaccaa attccaaata aatttcaatg
     2581 atcaaaatca aaatttgaaa attgggattt gaatattatt tggaatttgg tgcttgttat
     2641 ttgttgatta tttttgttat ggctcgacaa gctccccttg aaaaatatcg caatttcggc
     2701 gtgatcgccc acattgatgc cggcaagacc accacttcgg aacggatttt gttttacacc
     2761 ggtttgtcgc acaagatcgg cgaggtgcac gaaggcgcgg cgattatgga ctggatggcg
     2821 caggagcggg agcggggcat cacaatcacg tccgcggcca cgacttgttt ttggtcgccg
     2881 ggcgaatatc tgagcaagca aaaaaccgac caacatcaat tcaatatcat tgacaccccg
     2941 gggcacattg atttcaccgc cgaggttcag cgggcactgc gcgttttgga cggcgcggtg
     3001 gtggtttttg acggcgtggc cggggttcag ccgcaatcgg aaaccgtttg gcggcaagcc
     3061 gacaaatacg cggtgccgcg aatttgcttc atcaacaaaa tggatcgcat cggcgccagc
     3121 tttgagcgca gtttgcagtc aatctggaaa aaactgaatc ccaacgcggt cgcggccaac
     3181 attcccatcg gcgaagagga caaattgagc ggcgtggtgg atctgatcaa aatgaaggca
     3241 ttgcggttta cgggagccaa cggtcaggaa gtggtggaag aggaaattcc cgccgatatg
     3301 gccgaaatgg ccagcgcctg gcgcgccaag ctggtggaaa agatcgtttc cgaagatgaa
     3361 gatctgatga acaaatattt ggccggcgaa gaaattcctt ccgacgattt gcgccgcgtt
     3421 ttgcgatcgg ccaccatcaa gggcaatatc atcccggttt tttacggatc atcactgaag
     3481 aacaccggcg tccagccggt gctggacgcg gtctgctatt atttgcccag cccgcttgat
     3541 ttgccgccga tgaaaggaat ggaagtgcgc ggcgaagggg aagttttgcg tcatcccgac
     3601 gacaacgagc cgttcacggc gctggcgttt aaagttgccg ccgatcccta tgtcggcacg
     3661 ttgacttttt tccgggttta ttcgggcgtt ttgcaaaaag ggtcttatgt tttgaacact
     3721 gccaccggag agaaggagcg catcggccgc ttactgcgga tgtattccaa cgatcggaaa
     3781 gaaattgacg aagtgcgcgc cggcgacatt gccgcgaccg tggggttgaa aaacacttcc
     3841 accggccaca ctctgtgcga cgagagccat cccatcattt tggaaaaaat atctttcccg
     3901 gagccggtga ttgacattcg gattgaaccc aagaccaaag ccgatcagga aaagatgggg
     3961 tttgcgctca aaaagctttc cgacgaggat ccgaccttcc gcgtgaagac cgatcaggag
     4021 accggcgagg ttatcatcgg cggtatgggc gagttgcatc tggacatttt ggtggaccgg
     4081 atgaagcggg aatttaaggt ggaggcgaat gtcggccggc cccaggtggc ttacaaagaa
     4141 accgtgaaaa ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggttcgtt
     4261 ccacgaagtg gattcttcgg aaatggcgtt taagattgcc ggttccatgg ctctgcaggc
     4321 ggcggcccgg aaagccaatc cggtgcttct ggaaccgatt atgaaactgg aagtgacgat
     4381 tccgcaggag tttttcggcg acgttatcgg cgatctttct tcgcgccgcg gacgaattga
     4441 agaaacgcag gatcagatgg aatcaaagat cattgacgtg aaagtgccgc tgtcggaaat
     4501 gttcggctat gccacgaatt tgcggtcgct cacgcagggg cgcggaacgt tcacgatgga
     4561 gtttgatcat tacgaagaag tgccggccaa cgtggcgcag gaaattgttt ccggcaaacg
     4621 caagtaaaaa tttgactttt caaaaaattt gagtaatata taactgtcat tattttgtct
     4681 cgttaaaata cataataaaa aaactatggc ggaaaaagaa aaatttgtcc ggggtaaatc
     4741 ccatttaaac attggcacca tcggccacgt tgaccacggc aagaccacat tgagcgcggc
     4801 gattttgcac tcgctcggtt tgaagggcaa caaggtggcg atgaaatcca ttgaccagat
     4861 tgattcggcg ccggaagaaa aacagcgcgg cgtgacgatc aatatttccc atctggaata
     4921 tgaaaccgaa aagcggcact acgcccacat tgattgcccg ggccacgccg actacatcaa
     4981 gaacatgatt accggcgcgg cgcaaatgga cggcggcatt ttggtggtgt cggccgcgga
     5041 cggcgcgatg ccgcaaaccc gcgagcacat tttgttggcg catcaggttg gtttgccggc
     5101 gatggtggtg tttttgaaca aatgcgatca ggtggacgat ccggaaatga ttgatttggt
     5161 ggaatcggaa gtgcgcgaac tgttgaaaaa atatcatttc cccggggacg aaattccgtt
     5221 cgtgcgcgga tcggcgttga aagctctgga aggcaagtcc gcggatgatg aagcggtgaa
     5281 agcggtgatt gaactgatga acaaggttga cgaatacatt cccgaaccgg tgcgcgacac
     5341 cgaaaagcct ttcttgatgg cgatagaaga cgtgttttcc attgccggcc gcggcaccgt
     5401 ggcgacgggc aggattgaaa gaggcgttgt caaatcaaac gaagaagtgg aaatcgtcgg
     5461 aatcaagccg actgccaaga ccacggccgt ttccattgag atgtttcaga aaattttgga
     5521 tgaagggcgc gcgggcgaca acgtcggcat tttgttgcgc ggcacgaaaa aggaagacat
     5581 tgagcgcggc caagtgctgg ccaaaccggg atcaataact cctcataccg aatttacgtc
     5641 cgaagtttat attttgacca aagaagaagg cggacgccat actccgttct tcagcggata
     5701 caagccgcaa ttctatgtcc ggacttgcga tgtgaccggc gaagtgacgc tgaatcccgg
     5761 ggtggaaatg gtgatgccgg gggataccgt cactttcaac gtgaaattga ttaacccgat
     5821 cgctttggag gaaaagtcca aattcgccat tcgcgaaggc ggcaagaccg tgggtgccgg
     5881 agtggtaacc aagatcgtta aataaggaat taaaatcagc aagatggcaa ccgcgacaat
     5941 caagaaaaat aaagagacaa aggaggccgc ccgcccgaaa ttgagaatca aattgcgggc
     6001 gtacgaccac aaggtcattg ataactgcac caaacagatc attgagacga tcaatcgtta
     6061 tggcgccgag attttgggtc cgattccttt gccgatagag attcacaaat acaccgtcaa
     6121 ccgttcaaca tttgtgcaca aggacgcgcg ggaacagttt gagattcggg tgcacaagcg
     6181 cctgttggac attatgaatc caacgcccaa gatcattgat tccttgcgca atttgacttt
     6241 gccggcgggc gtggacattg aaatcaaaat gtagccggcg cgcgccgttt gccattgcca
     6301 gaacaagaat atagccattc agaagacccg ggctatgccc ggttttctat tgccttcaac
     6361 aaagattatg aaattcattt tgggaaaaaa agtcggaatg acggaaatgt ttgctccgga
     6421 cggaaagaaa atcccggtga ctttggtttg cgccggtcct tgcacggtga cgcagattaa
     6481 gaccgccgaa agagacggtt atcgcgcggc gcagattggc attgaagaaa ttgccgcggc
     6541 caaagttgcc aaaagccgcc aaaagaaacc gtttcgattt ttgcgggaat tccagattga
     6601 tgctgttgcc gaattgaagg cgggagacgt tgtttccgtg gcgaattttc aggaaggcga
     6661 caaagtcaaa gttgccggaa tttccaaagg caagggattc cagggcgcgg tgaagcggca
     6721 cggattcaaa ggccagcaaa cctgcactca cggcacgaag cacgaattgc gcaatctggg
     6781 atcggtcggt atgggcgaag cttccattcg caagggcaga aagatgcccg gcagaatggg
     6841 cgccgaaaga atcaccgtcg ggaatttgaa gattgtcaaa atcgatccgg aaaacaattt
     6901 gctggcgttg agaggcgcgg ttccgggaaa caagggaact ttgctggaaa tcagaggaag
     6961 ctaagttaat tatgatatta ttccttaatt tttaactttg aattttgaat tttaaatttg
     7021 aaacatggaa gccattgttt ataatcaaaa aggagagaaa ttgaatgcgg tgaatttacc
     7081 cgagggcgtg tttggcgtga aaatgaatcc tgatttggtg taccaggtgg cgataactca
     7141 ggcggccaat cgtcgccgga taacggccca taccaaagac cggggagaag tttccggcgg
     7201 cggcaaaaaa ccctggcgcc agaaaggcac cggccggtcg cggcacggtt ccaaccgttc
     7261 gccgatctgg cggcacggcg gcgtggtttt cggccccaga aacgataaag ttttcggcgg
     7321 caagatcaac aaaaaaatgc gccgcaaagc gctggcgatg gttttgtcgg ccaaagcttc
     7381 cggcgggatg atggtcttgg ttgataagat tgagtttgac gtgccgaaga ccaaagttat
     7441 ggcgcagatg ctggaagcga taaaaaaagt gaacgagaat ttcaaaaccg gaaaggtttt
     7501 ggtcgccctg cccgaatttg aaaaaaatgc cgtcttgtcg ggcaggaata ttcccggcgc
     7561 cgagatgatt gaagcggcaa aattgaacac ccttgatttg ttgaatgcca aatgcctttt
     7621 attgccgtcg gcggcggtga aggtggtgga acggattgtc gccggcggtg aaaatgaaaa
     7681 gagtgagaaa aaatccgaaa agcaagaaaa atccgaaaga aaaccgtctg cgaaaaaaac
     7741 taccaagaaa taaattttat tttttcaatt ttaattttta atttggaaca tggcgattac
     7801 cgacattttt aaaaaagaag aggcccaaaa aggcaaaaaa aagccgggcg ccaagcctgc
     7861 cgcggaaaaa acggataaaa aagaagcccg ccagccgaaa aacgccggcc gcgcgatgag
     7921 agttttggcg ggcgtgcacg cgaccgagaa agcggcgaat atggccgaag aggaaagata
     7981 tgtttttaaa gtggctaaaa ccgccggcaa aaaagaaatt gcccgcgcgg tggaagatta
     8041 ttacggcgtg gatgtggtga gcgtgaatgt gatcaacatt cccggccgca accgccgcaa
     8101 ccgccgcggc atctattttg aaagcggcta tcgcaaagcg gcggtgaaga ttaaaaaagg
     8161 gcaaaccatt gaggcggcgg cgaccaaata gcccgcccgg cggtcgttga attttatcat
     8221 tttattttat gaaatccatt ttaagcaaaa aaaggccgga aaaacatctg ctgaaatcgt
     8281 tgaagagcaa cgccggaaga aatttttcgg gccggatcac cgtccgccat cgcggcggcg
     8341 gcgtgaagcg gatgtatcgc atggttgatt tcgggcagga aaaattggga attgagggcg
     8401 gcattgtcgc gctggaatac gatcccaacc gcaccgccta cttggcgctg gtggaatatc
     8461 gggacggcac ccgccgctat gttctggcct gcgaccagtc tttggccggc gacaagattg
     8521 tttgcgaaga aaacgccgaa attaaaaccg gcaatcggtt gatgttgaaa aacattcccg
     8581 tgggcaccga agtttgcaac attgaattgc ggccgggaaa gggaggccag ctggcgagat
     8641 ccgccggatc gtcggccaaa ttgatggccc atgaaggcaa attcgcgcag ttgcaaatgc
     8701 cttccaagga aattcgccgc gtgccggcgg aatgttttgc caccatcgga aaaatttccc
     8761 atccggaaca ccgatttgag gattacggcc gcgccggcgc caagcgccgc aaaggatggc
     8821 ggccgacggt gcgcggttcg gtgatgaatc cttgcgatca tccgcatggc ggcggcgaag
     8881 gcagaaccgg tattggcatg aaaagagcca aaaccccgtg gggtaagccg gcgatggggg
     8941 tgagaacccg caatcgcaac aagtggacga gcaannnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnggggta agccggcgat gggggtgaga acccgcaatc gcaacaagtg
     9121 gacgagcaaa ttgattatga agagaagaaa atagccaaaa atcggaatca atttaatttt
     9181 aaatttgaaa tatggcaaga agcatcaaaa aaggtccttt cgtggatgaa aaattaatgg
     9241 aaaaagtgaa aaagggcggc ggcgccgtca tcaagacttg gtcgcgccgc agtaccattg
     9301 tcccggaaat gatcggcgtg gttttcgcgg ttcacaacgg caaagaattt atcacggtca
     9361 ggcccaatga agatatggtg gggcacaagc tgggcgaatt ttcgccgacg acaaaattcc
     9421 atcgccacgg cggcaagatg caaaaagatc ttgaaaccaa gcaggctccg gtgaaaaaat
     9481 aagtcaaatt ttaaatttga aacatggcaa ttaccgtcaa attgagacaa ttgaagattg
     9541 cggagagaaa aacccgcatg gtggccgatc tggtgagata taaaaccgtt gaagaagcgg
     9601 aagcgatttt gagttttacg cccaagaaag ccagtttgcc gctcttgaag cttttgcgat
     9661 ccgcggcggc gtcggcaaag aacacctata aatttgatcc gaaaaatctt tatattgcca
     9721 aattgacggt tgacggcggc ccgatgcaaa aaagagtttt tccgcgctcc cggggacgcg
     9781 ccgaccacat tgaaaagcgg acttcgcaca tcacgctggt gcttgaacag aaattggaga
     9841 ttgagaacaa aaaataatcc gctttttaat tttgatattt taattttaaa tttgaaacat
     9901 ggctcacaag gttcacccaa aagcattcag aataaaggac gcctccgatt ggtattcgcg
     9961 ggggtattac ggcgaaaaat ttccaaaact gctggaagag gattttaaaa tccgcgagtt
    10021 tttgcatcgc cgcctggctg gcatcggcat ttcggaagtg gagattgaaa gattccccgg
    10081 cagaatcaat gtgctggttt cctgcgtccg tccggggctg atcatcggcc ggcaaggcca
    10141 ggggattgaa gaattgcgcc aaaaattaca gatgctggtc ttcggcggca atgccaagaa
    10201 aaagggtggt gccgatttga aggtggaagt ccgcgagatc gccagcccgt gggcttccgc
    10261 ggcctatatc gggcaatcgg tagcctccca gattgagaaa agaatgccct atttgaaagt
    10321 gacgaaacag tctattgaga aagcgatgac ttcccgggaa gtggaagggg tgaggatcca
    10381 gctttcgggc aggttaaacg gcgtggagat tgcgcgcacc gcgtggctgg ccaaggggcg
    10441 cctgccccgc cagaccattc gcgccgatat tgactatgcc atcaccgaag cgcattgcac
    10501 ctacggcgta atcgggatca aggtgtgggt ttataaggga gaaaaacaga aataaattaa
    10561 aaactgagaa cgaaaaagga aaaacgaaaa ttataaattt aaaattatca aattcaattt
    10621 ttaatttttc atttttacct tttaatttgg aacatgtctt tattgatgcc caaaaaaatc
    10681 aaacatcgga aatggcaaaa gggacgccgg cgcgggttgg gagtcgcatt gcgcggcgcg
    10741 gaattgaact acggcagttt cggattgaag gcgatggaag aaaaatggat tacttcgcgg
    10801 cagattgagg cgggccggcg cgcgattttg agatatttga aaaaaggcgg caaattgtgg
    10861 atcaggatct ttccggacaa gcccgtcacc agcaaggggg aagaagtgcc gctggggggc
    10921 ggcaagggcg cggtggatca ctacgctttt gtggtcaagc ccggcaggat tatgtatgaa
    10981 attgacggca tcaaggaaga gatggcgcgc caggcgctgg aaaaggccgg cgacaaactg
    11041 ccgatcaagg ccaaattcat cagaaaggaa gcgtaatttt taaattttaa aaaaaatgaa
    11101 agccgccgaa ctgcgccaaa aaaatattgc ggaactggaa ggattgcttg aagaaaagcg
    11161 ggcgaggatt gcccggctca gatttgattt ggaaggaggc aagaccaaaa atttgaaaga
    11221 ataccgccaa atcaaacttg atatcgcccg cgttaagaca ttattaagcg agggaatgtc
    11281 tgcggacaaa aattaaatta agagctgata caatttttcg ttttacgttt aaatttggaa
    11341 tatgaaacat caactaaccg gaaaagtcgt ttccgacaaa atgcaaaaaa ccgtggtggt
    11401 gatgaccgaa agcctcaaag agcacccaaa ataccacaag cgctaccgcg tgagcaaaaa
    11461 atacaaagcg cacgacgaga acaacaaatg caaaatcggg gacgtggtgc tgattgaaga
    11521 atgccgcccg ctgtccgcgg ataagagatg gaaggtggcg aaaaaaatcg ccgcgggccg
    11581 ggaagaagag gcggtcaaag ccgcgggcgc gaaagaagag gagtgattga aagtgcccgc
    11641 attgattttt tttccgattc atagtatata aatgaagttg cggtaatttt aagccgattt
    11701 ccgaaattat gatccaattg agaacaatgc taaaagtggc ggacaacagc ggcgccaaga
    11761 tggtgcagtg tttccacgtc gccggcggca cgagacgccg gtatgcggcg gtgggggacg
    11821 tgattatggc cgcggtcaag atcgctgagc cgcgcaagcc ggtcaagaag cacgataagg
    11881 tccgcgcggt ggtgattcgc cagaaaaaag agtatcggcg cgaggacggg acttatatcc
    11941 gttttgacga caacgcatgc gtgattttgg aaggcaaggg aaaagaaccg aaaggcggcc
    12001 gtattttcgg cccggtggcc aaagagctga aagccagagg atatgacaaa atcgccgcta
    12061 tggccgagga actcatataa aatcaaaatt aaggaagaat tgcatatcgt tgaaattttt
    12121 tgcaaaaaat ttcaactcca aaatttttca ttttgatatt tcaattttaa atttttagga
    12181 tgaagattat taaaggcgac aatgtgttgg tgatatcggg caaggatcgt ttaaagaccg
    12241 gcaaggtttt gcggtctttg ccggcagtgg gacggctggt ggtggagggc gtgaatgtcg
    12301 ccaaaaagag ccggcggccg agaaagcagg gggaaaaagg ccagattgtt tccctgccga
    12361 tgccgattga cgcttccaac gtgaaactgc tgtgccccaa atgcggcaag ccggcgcggg
    12421 tggcatacaa ggcggcggaa ggccgcaatc ccaaagaaaa gaacaagacc agaatttgca
    12481 aaaaatgttc ggccgaaatt tagcgagcgg ttgaggaaat tatcttttat tatggcgggc
    12541 ttatacgaaa aatataaaaa ggaaacggtg ccggcgctgg tgaagcagtt cggctggcgc
    12601 aacgaaaatc aggcgccgcg ccttgaaaag gctacggtca attgcggttt cggccggatg
    12661 gtttcgggaa aaacaaagga tgagcagaaa aagatccagg aagcgattgc ccgcgatttg
    12721 acgcagattt gcggccagcg cgcggtgatt tgcgcggcgc gcaagtcaat tgcggggttt
    12781 aagatccgcg aagggcagag cgtcggcgcc aagatcactt tgcgccgccg ccggatgtat
    12841 gattttttgg aaagagcgat taccgtcgcc ctgccgcgta cccgcgattt tcagggaatt
    12901 ccttccaaaa acgtggatcg ggacggcaat ttgactttcg ggattaaaga acacatcgcg
    12961 tttccggagg tttctcccga aaagataaat ttcattttca gttttgaagt taccgttacc
    13021 accacggcgc gcaaccgcga ggaagcgctg gcgatgttta agtcattggg gtttcccatc
    13081 aagaaagatt gactgccggg attgaataat ttttaaactt ttcgttttta attttaaatt
    13141 tgaaacatgg cgacaaaagc gcaaatcgca aaatccaaac gaacaccgaa gttcaaatca
    13201 cgcgtggtgc ggcggtgttt ccgttgcggc cgaaagcatg gctatatgag aaagttcgga
    13261 ttgtgccgga tatgcttccg cgaagcggcc aatcagggat tgattcccgg cgtggccaaa
    13321 agcagttggt gataaaaaat taaaaataaa cattaaaaaa tcaaaattaa ggaaaattgc
    13381 atatcgttga aattttttgc aaaaaatttc aactccgaaa cttttatttt gatatttcaa
    13441 ttttaaattt gaaacatgga taaagtagca gacatgttaa ccgtgattcg taacgcgcaa
    13501 gcggccggca aaccggcggt tgccgttcct tattccgcgt taaaatacaa tattgccaag
    13561 attctggaaa ggcggggcta tgtcgcgacg gtggaaaaaa gaagccgcaa ggcgaaaaaa
    13621 aacggcaaag cgcgcccttg tctggagatt tctttgaaat atgttgatag cgtccccgcg
    13681 atcaccggat gcgagaaagt ttcccggccc ggccagcgga tttacgttcc cgccagcgat
    13741 atcaagaggg tcaagcaggg caagggaatc ggcatcattt ccacttccaa aggattgatg
    13801 accgaatatg atgccaagaa acaaaatatc ggcggcgaaa tactgtgcca gatatggtaa
    13861 aaccaaattt aaatttcaag aaattatgag tcgaattggt aaaaaaccgg taaatattcc
    13921 cgcgggagtg acggtggagg taagcggcga cgaggtaaaa atcaagggtg ccaaaggtga
    13981 attgtcgcgc cgtttcgggt ccaaagttgc gattgtccgg gagggggacg cgctgaaagt
    14041 ttcgcccgcc gagggaagca aggccgcccg cgccatgtgg ggcaccgtca gaatgcactt
    14101 ggcgaatatg gtccaagggg tttgcgccgg ttttgaaaag caactgcaaa ttgaaggaat
    14161 tggctatcgg gccgcggttg aggggaggga tttggtttta aatatcgggt tcacccatcc
    14221 ggtgaggatc gccgcgccgg ccggtattgc gtttaaagtg gaaaagaaca ttgttacggt
    14281 ttcgggttgc gacaaggaaa cggtgggaaa gattgccgcg caggtccgcc gggtgcgccc
    14341 gccggaacca tacaaaggca aaggcatccg ctatgtcggc gaaaacgtcc ggcgcaaggc
    14401 aggcaaaaaa gccgccgggg cttccaaata attaaaacga aagaaaaacg gaaatctatg
    14461 agcatcagca acaaaacacg ccataaattc gtgagaagag ccaatcgcca ccggcgggtg
    14521 cgcgccagga ttgccggcac cggcgaaacg ccgcggcttt gcgttttccg atcaaatcgg
    14581 catatttttg cccagttaat tgatgatcgc cggggaaaaa ctttagccgc ttgcggcgac
    14641 ggggatgtga gtgccgaaca gaccgcggaa aaaagcgatt tgtcgggaaa atgcgcttcg
    14701 gcgtttgcgg tcgggcgccg gatcgcccgc cttgccggcg aattgaaaat ttcccgtgcg
    14761 gtgtttgatc gcggcggtta ccgctatcac ggcagagtca agagtttggc ggaaggcgcg
    14821 cgcggcggcg gattgaaatt ctaattttaa attttcattt ttacttttta atttgaacat
    14881 ggaagcatca caaacaactc cagttaaaag cgaagcggca aagccggcat tcaagccgtt
    14941 cccgtccgcc ggtgcgcggc cgcaatttaa ccgcaatcgc ccggggcagg gcggcggcgg
    15001 ttcgtctcgg tttggcgccg ggcgtttcgg cggccgtggc cgccaaggcg gcggacggcg
    15061 cgatgatggc ggacggaaag acgaatttga ttccaaatta ctggacttat cccgcgtgtc
    15121 gcacacgcgg gcgggcgggc gccgtttgcg tttccgcgcc attgtgatta gcggcaacaa
    15181 agccggcaag gtcggtttgg gtgtggcgtc ggggctggat gtggcgcaag cggtggaaaa
    15241 agccacggtg caatcaaaaa agaagatgat cgttgtgccg acggccggag gaacaatctc
    15301 ccgcgaggtg gcggccaaag tcgggccggc gcgggtgatc atcaagccgc aacgcaaggg
    15361 ccgcggattg atggccggcg gcgtggtacg cttgctctgc cagctggcgg ggatcaagga
    15421 catttcttca aaaatgctgg gcacgaccaa aaacaaactt aataacgccc gtgcggtgat
    15481 gagagctttt gaagatttgt cgcggcaatc gcggaaggac tccgcgcaaa atccggagcc
    15541 gggggcccaa aacaaaattg aggccgaaaa agaaaatccg aaaaattcac cggccgcgga
    15601 cggagcggcg aaagtttcac agtaggttta ataattccat gcagatacat caattaaaaa
    15661 attcttccgg cgccaaaacc cgaaaacgag tcggccgggg aggcaaacga ggcacctact
    15721 cgggccgggg aggcaagggc caaaccgccc gcgcggggag aaatttccgc ccgataatca
    15781 gggaagtgtt gaagcgctat ccgaaactgc gcggccatcg caaccgcgcg gccggtttga
    15841 cggcttttgc cgtcaatctt gcggtgctgg aggccggatt tgaaaacggc gcggccattg
    15901 atccccgggt tttgattgag aaaaacattg tgcgttgcgc cggcggcaaa actccccggg
    15961 tgaaaatact ggggcgcgga gagatctcaa aaaaattcaa tatctcggga tgcgagattt
    16021 cggcggccgc aaaagcgaag attgagaaag ccggcggcgt tgtcaaccaa gccgttttgc
    16081 cgccaagtgg cgaagcattt tcttaaaata aaaatcaatg tggtaccagg cgatccttca
    16141 gccgtttaga gtgaaagaac tgcgcaacaa gatatttttt gtgctgggcg cgtttgcggt
    16201 ttttcgttta atggccaaca ttccgatcac cggcgttgat acgaaccaat taaagcaatt
    16261 cttcggcgaa ttcgggattt tccagctgtt gggcgcgttc accggcggtt cgctggatcg
    16321 tttttcggtc gcgatgctgg ggctggggcc ttacatcacc gcgatcatta tttttcaatt
    16381 gatgacgatg atttttccgg cgctggaaag gctttataaa gaagagggtg aagcgggccg
    16441 cgccaaattt aaccaatacg cgcgcattgc caccgtgccg ctggcgttat tgcaggggtt
    16501 tgcgatgatc gccctgcttt ccgggccgaa cgtgggtatt ttgcattttg attcgttgct
    16561 ggaaaagatc gcggcgctgt tttccatcac cgccggcgct ctgcttttga tgtggatcgg
    16621 ggaattgatg acggaaaaag ggattggcaa cggagtgtcc ctgttgatct ttgccggtat
    16681 tatggctgat tttccgcgca acattcgcca gatgattgaa gtctacgatt cttcaatggc
    16741 gctcggatac atcgcgtttt tcgtgatggc ggcggcgatg gtggccgcgg tggtgctcgt
    16801 gaacgaagcc cggcggaaca ttcccgtttc ttacgctaag cgaatgcggg gcacgaaaat
    16861 gaccggcggc gtttccacct atctgccgct caacatcaac cccgccggcg tgattccgat
    16921 cattttcgcg ctttccattt tgatctttcc gccgatggtg gcgcaattct tcgcgtcttc
    16981 cggaggatgg gtcggcgatg ccgcccgcgc cgtggaagga ctgtttgcca tggacggcgc
    17041 attctatatg atttcttatt tcgtgctggt ggtgttgttc acttattttt acaccgcggt
    17101 gacttttgat cccaagcaaa tttcaaaaaa cttgcaaaaa atgggcgggt tcattccggg
    17161 tatccgcccg ggacagccga cggcggatta tttgcgctat attttaaacc gggttctgct
    17221 tttgggggcg gtgttcctgg gcttgatcgc ggtgatgccg cagattgtcg gaccgttcac
    17281 gggagtgact tcattttcgt tcctgatcgg cggcacgtcg ctgttgatcg cggtgagcgt
    17341 gattttggaa accgcgcgcc aaatcaaggc gcagcttcaa atgcgcgaat atgaaaattt
    17401 ttaatcgggc aattttaaaa aatctcaatg gcaatcaaat ccaaaaagca atcctgcttt
    17461 ttgggcctcc cggggcgggc aagggcacgc aggccgagct tttggcgtca aaattttatt
    17521 tttacaattt ggataccagc cgggtgctgg aagaaaaatt caaaaacgcc aaacccggcg
    17581 cgcaggtcaa gattcataat gccgtttatc cggtggccga cgagctggcc aaaagaaaga
    17641 aaggcgtgct ttgttcggtg gagttcacgg cctttttgat gaacaaaagg attgccgaaa
    17701 ttcacggctt ggggaaatcg ctcatcttgt cggggtctcc gcgatcgctg gccgaggccg
    17761 agattgaaat gccgtttttg gaagaacttt acggcaagga aaacatccac attttgtttc
    17821 ttgatttgcc ggcggagcag acgattttcc gcaacagcaa tcgccgcatt tgcgaattga
    17881 tgcgccattc catcttgtat attcccgaaa acgccaattt gaagcgctgt ccgttggacg
    17941 gttcgcgctt gatgcaccgc gacttggaca atgttgaaac catcaaggtg cgtttggaag
    18001 aatttgagca gaccgccgcg ccgttggaag attattaccg ccggcacgaa tataatttga
    18061 aaaaaatcaa cgctgatcaa tatgtggaaa atgtttttga cgatattatt gccgcgctgg
    18121 ggccgggttt taaagtttat gaaatgtaat taaacgcccc gaagggggcg ttttgcgatt
    18181 gccgatgatc aaaatcaaaa gtccggaaga aatcgggatt atggccgaag gcgggcgcct
    18241 tttggcggcc gttttggaag aactgaaaaa gaaaaccgcg gcgggagcgt ccaccaagga
    18301 gctggacgat ctggcggttt ccctgatcgc cgcggccggc ggccggccgt cttttttggg
    18361 cgaaggcgga tatccggcga caatctgcgt ttccatcaac gatgaagtgg tgcacggcgt
    18421 gccgtctccg aaaagaatta tcaaaaacgg cgacatcgtg agtttggacg cggggatgat
    18481 tcaccgcggc tttcataccg atatggcggt taccgtggcg gtgggcgagg ttgatccgga
    18541 attgcggcgg atgatcaaag cggctaaaaa agcgttaaaa tacgccatta aaaaggcgcg
    18601 tccgggaaac acctttggcg atgtgggcaa cactgttgag cgttacgtgg aaagccaggg
    18661 atttcacgtg gtgcgcgatc tgtgcggaca tggcatcggc aaaaaattgc acgaagatcc
    18721 gcaaatcttg aattacggcc gccggcatcg ggggccggtc ataaaagaag gtatggtttt
    18781 ttgcgttgag ccgatgatca ccgccggcaa tccggagatt attttggcat cggacaaaca
    18841 agcctataaa atcaaagacg gcagtcccgc ggcccattgg gaacacactc tggcaatcgt
    18901 caatggcgcc tgccgggtgc tcactgcctt ccccgatacg gaggacaaag aggattgaaa
    18961 aacggagcag tctccgtttt tttgttaaac gggcgggttt gtgataaatt gataatgaga
    19021 tttaaacgtg atttcaatat gaaaaaaaat caatgctgtc cgtcgcaatt gcgctatgac
    19081 gtggtgtcca aagactgggt ggtgatcgcg ccggggcgcg gcaagcgtcc ggaagaatac
    19141 aaacggcaaa gaaaaatcat tgaaatggat ttgcggcaat gtccgttttg caatctcacc
    19201 gaggccgccg atccgcttct ggttttttcc gccgggaaaa tcgccgccaa aaaaacattg
    19261 cccggcgatt ggacgctggc ggtgattcca aataaatttc cggccttcta tcccattacg
    19321 gggaaaagcg tttgccagtg gaagatcggg ccggtctacc gttcaatgga cgcggtcggt
    19381 ttttgcgaag tggtggtcac gcgcgatcac gattgccatc tcggactgat ggatttgccc
    19441 gcaaccgaag aagtgatggc ggcttaccaa acccgttatt tggaattgaa agagcacaag
    19501 ctggtgaaat atatttcaat tttccacaat cacggcgccg aagccggcgc gtcccaacct
    19561 catccgcatt cgcaaattat taccagcccg ctgattgacg ttgatctctt gggtgaattg
    19621 gaaaattcaa aaaaatattg gcggacgcat aaaaaatgtc tggcgtgcga gatgaacaag
    19681 tttgaactca aagatcgttc ccgcgtggtc tgcgagaacg cggatttcgt cgcttcttgt
    19741 ccgtttgcct ccaagacggc gtttaaagtg gtgatctctc ccaaattcca ttcgccgcgg
    19801 tttgagaata ttgatccggg acagagaaaa tctttgacgc aaattttgtc ggcggcgatc
    19861 aaaaaaatct ataacgcctt gggtgatccg gcctataatt tttatctgca caccgcgcct
    19921 tgcgaccgca aagactattc ccattatcat tggcatctga ccatcgtgcc caaaaccgcg
    19981 attatggccg gctttgaaat tggcacgcgc attgaaattt ccaccattga accggaacgc
    20041 gccgccgaat acctccgcgc ccaatagtat tggttgactt ttggttgtga aatagtatgt
    20101 ttaaatcacg attatcaatc atttgaatca tttgaattat atatgaaaac atacactttc
    20161 agagttatta ttgaaccgga cggcaaagcc ttccacggct atgtgccgat tttgcgcggt
    20221 tgccatacct gcggcaaaac attggaggaa accaaaaaga atctgaaaga agcgatcaag
    20281 tgccatgtcc aggggcttgt cagggacggc gaaaatattc cgcaagagga aaatgcgttt
    20341 gaattggtgc aaaccttcac cgataaggaa ctggcgttga cttgttaaaa tatggcaaaa
    20401 ttgccggcgg taaaagcgca aaaattgatc aaagttttgc tcaaactcgg ctttttcaag
    20461 tatagccagg ttggtagtca cgcgcaattt aaacatcccg gaggccggcg gaccacggtg
    20521 ccgattcatc aaggaaaaga gatttcaccc gggacattgc tggcgatttt aaaagatatc
    20581 gaagtttcaa aagacgattt tataaaactt ttgtaaaatt gctactactt aaaatttgat
    20641 ttgattaaat atttcctgtt gtgatttcca tatttcggct acgaaaaatt cgtcagtcgt
    20701 cggcaggttt ggagtgaaac gagttttact cccgcaaact aaagccgagt atgcctcctt
    20761 ctttcttaaa attaaaatat gaaatatatt gttgggaatt ggaagtgcca tccggccaat
    20821 aaaattgagg ccaaaaaaat acttttggga tataaaaagg gggtcaagaa cagcgccaag
    20881 gccaaggccg tgatctgtcc gccggcgtgc tatttggagc tggccaaaaa gattttaaaa
    20941 aattctgcgg agttgggcgg ccagaattgc cactgggaag agggagcgtt caccggcgaa
    21001 atttccgcaa cgcagttaga aagcgcgggt tgcaaatatg tgattcttgg ccattcggag
    21061 cggcggcata aattcggcga aaccgatcag gcgatcaaca aaaaaattat cgccgcactg
    21121 acggcgggat tggtgccgat tttttgttgc ggcgaaaccc gtgaacagcg cattgacggg
    21181 caaatcaccg acattgtgga aacgcaaatt caggaaggcc tgaaaggcat tgacttaaat
    21241 tccgcgccgg taatgattgc ctatgagccg gtgtgggcca ttggcacggg ccaggcctgc
    21301 gattccgaag aagccgcggt gataagccgc ttcatcaaaa acattaccaa tgaaaaaatt
    21361 ccgctcttat acggtggttc ggtaaattcc aaaaatgccg catcctacat caaaaacgcc
    21421 gactatgacg gccttcttgc cggcggtgcc tctttggatc ccaaagaatt ctctgccatc
    21481 ttcaccaccc tgcattccct ttgacggaat taaaaccaag attattagag aacaaaaata
    21541 aaatcctgac aaattttgcg accgcaaaat ttgtcaggat ttgccgaatt ccaaatttca
    21601 atttttaatt ctatgattta taaatttacc accattataa ttcgtgaagg aaaatggttt
    21661 gtcgcgcgtt gcgcggaatt gggagttgtg agccaaggca agaccgtttt ggaggcaaag
    21721 aaaaacatca aagaagcggt tgagctctat ctggaagaca atccaaaatc aaagaaacag
    21781 ctttcttccg gaggcgcgcc tttaattgcg cgtcttgaat tggaatatgc ctaagaaagt
    21841 tttctcaggc aaagaaacaa tcagaatttt atgccgtgaa tttggtttct attttgtttc
    21901 gcaaaaggga agccatgtga aactgggaaa aatgagtggc aagcgggaaa ttattactgt
    21961 tgttccgttg cacaaagagc ttgctattgg cacattcaga ggagttttgg aattagccga
    22021 aattgaatta aaagaatttt tatcaaaatc tgacagatcg tttcgctaac aatattccaa
    22081 tattctgcag aataccagaa tagtttgtcg tcaaggcggt taacggatat tccaacatcc
    22141 ttgagaatgt tggaatgttt tgttattggt aaccactcgc tggggggatt gtattttgag
    22201 ggtctatccc tcgcgcttgt cccgaggtaa tccgagggat aatcgcgagc ggttagaccc
    22261 tggcaaagaa ttgcaagcac aaaattaaaa gggtctaacc ctcgttactg cgagggatag
    22321 acccttttaa ttttttagac ccagtgaatg tggttgccgt aaacatattt gcttaaattt
    22381 ttgaaagttt gtaaaatata atcaaaagaa atataattat tatgcggtct cataataatt
    22441 aacaatgccg cagtaagcgg cgatataaaa aaatgaatta ccaaaattca aaaggtgttg
    22501 tttcaataat tatcttggcg gcggtcgcca ttgccggagt agttcttgtt gatcagtttt
    22561 cccgcatcaa tgaaagaatc tcccgcttgg aacaagcaaa ccccgccgcc gctttattga
    22621 gtccggcgga aacggctaac ggcgatagca tcggcgccca aggaattgcg ggcgaaacca
    22681 aggccgaaga ggagcccgtc ggagaagtaa tcgtccccga cgattcggtg acgatcagcg
    22741 gaaaagtggt caaggtcgaa aaagacgccg taaccatcac aatgatgccg gggggcgaag
    22801 attatgattt tgtcgcgctc gtcaatgaaa ataccttcgt gaacaaacag aaagacgccg
    22861 ccgatcaaaa ggcgtctccc gccgaaattt ccgaaataaa aatcggcgat tttgcggtca
    22921 ttgagagcgc cggacaaatc ggcgatgaca aaaaattcac ggcaagaaaa atcaattttt
    22981 ttgaaatgcc cgaggaacaa ccgcaaagcc aacaataatc aaaaaaagtt tccgcgattt
    23041 ttttctccgg cggaaaccga tcaataatca aaacgtttat ggaaaaaaac aaaaatcaaa
    23101 gcgtttccaa gattgtcccg cttacgttga gcgtggtaat aatctcggtg atgatgtgct
    23161 tatccattac ggcctagaac ccgcccgacc aaactccgcc cggcaataac gccaaagcac
    23221 caataaacgt cggcgacgcc gcacaaggga aattgggcaa tctaggcatc ggtaccgcca
    23281 gccccggagc aaagcttgat atcgcggac
//
LOCUS       DAOQWE010000002        17886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_3540_length_17886_cov_7.321855, whole genome shotgun sequence.
ACCESSION   DAOQWE010000002 DAOQWE010000000
VERSION     DAOQWE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17886
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_17886_cov_7.321855"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKK37_00195"
     CDS             <1..658
                     /locus_tag="PKK37_00195"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNR80838.1"
                     /translation="VTLQFLIMLWPVWLIFGIVIFLKFLFVILIPEWLERRRNHKRFV
                     EGEEWRSDRDLIYWLRGMKPDEFENYIADLFSRLGFKTEVVGGSHDGGIDVVAEQNGI
                     KHYIQCKKFINHEVGVGAVRDFYGAVADHLAQGKGYFITTNKFTLEAEKFAQGKPVEL
                     IDQFRLVEYIRLAKKDISDDRKQKICSKCGGNLVERAGKYGKFFGCENYPKCNHTEKF
                     "
     gene            828..1136
                     /locus_tag="PKK37_00200"
     CDS             828..1136
                     /locus_tag="PKK37_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80839.1"
                     /translation="MAENKLSEDQILSIIKGEREKLSNPYGDQTDLVVDFAVGLYERK
                     EIDRVVFKTLISLAILKLTQKTVADLMDNLIKRNLISKKSGSDSDEFVLLNYFKKNYG
                     "
     gene            1129..1503
                     /locus_tag="PKK37_00205"
     CDS             1129..1503
                     /locus_tag="PKK37_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80840.1"
                     /translation="MDKVIQSSDTDESRQSGRESGKTRVVSESLQMLMASLGGSQDLP
                     VTEEHISEMLSQRRQVNEFVNEGKKRDSYDQRYCLTIGLLFIVVISALIIFTHPEYFT
                     QVLSLIIGGFGGFGLGRASKKD"
     gene            complement(1559..2614)
                     /gene="prfA"
                     /locus_tag="PKK37_00210"
     CDS             complement(1559..2614)
                     /gene="prfA"
                     /locus_tag="PKK37_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNR80841.1"
                     /translation="MPQALSHQEEYDRLLVSLGDPGLISDWEKFEELNKRKIQLEKII
                     EKENEIKEIANKIEENRLIIAAEEDPELVSLAEVETEQMKIRKTELENELRDLLKDEG
                     KSVGPGAIVVEIRAGAGGQESALFAANLYRMYSRFAQEQEWKQKVLDQNQTEIGGYKE
                     IIFELKGRGVWPAMRHEGGVHRVQRIPETEKSGRIHTSTATVAVLPKPKKNDISIRSD
                     EIRVDFCRSSGPGGQFVNKRESAVRITHIPTGLSVFSQTERNQLQNRENAMALLEYRL
                     QEKKRQEEESAFGGARKEQIGAGERCEKIRTYNFPQDRVTDHRIKKNWHGIEKILDGG
                     LADISQTLQDELDKTPL"
     gene            complement(2712..2945)
                     /gene="rpmE"
                     /locus_tag="PKK37_00215"
     CDS             complement(2712..2945)
                     /gene="rpmE"
                     /locus_tag="PKK37_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNR80842.1"
                     /translation="MKKDIHPKYYPNAQIKCSCGQTYQIGSSKEYLETETCAACHPFY
                     TGKARLIGAMGRVDKFLKKVAKKDALKAGKKSK"
     gene            3140..3871
                     /gene="rpsB"
                     /locus_tag="PKK37_00220"
     CDS             3140..3871
                     /gene="rpsB"
                     /locus_tag="PKK37_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNR80843.1"
                     /translation="MAVKLSVEEMTEAGLQFGHRKSKIHPKARGFLLGVRNTIFIIDL
                     EKTKEKFAAALDFARSLASEGKTIVLVGTKPQVKKMIKETAAACEMPYVNERWLGGTF
                     TNFKIIKKRIDHYKDLEKKTLAGEFEKYTKKEQAMIQKEIKVLEKKLGGIRAMEKIPE
                     AIFVVDMEDDRLAVKEAKQNGVKVIGISDVNIDPNIADYPIIANDDSMSSVRFVLERM
                     KEAILEGKGMFVASVSAGASEKNKN"
     gene            3963..4406
                     /locus_tag="PKK37_00225"
     CDS             3963..4406
                     /locus_tag="PKK37_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNR80844.1"
                     /translation="MVDIELIKQLREETGISLGECKKALEEGKTVEKAKEILKEWGKD
                     LAAKKEDRQTGQGKVASYIHANGKIGVLIELRCETDFVANSADFKSLSHELCLQVAAM
                     GGEAENIPGQPWIKDSSKNVKDVISETVAKVGENIVLKKAARFQI"
     gene            4422..4988
                     /locus_tag="PKK37_00230"
     CDS             4422..4988
                     /locus_tag="PKK37_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80845.1"
                     /translation="MVEIIASIALIGGIAGAGSIIARKMPQAVKTLEADRAAFPAGVF
                     SGLKNWIVGRIKRRSSMKDFSWIDFSQKQLLKIRVAVLKIDNKINEIAGKLRQRAEKK
                     RQEEQIAGDNYWQDLKNVVKAGQSASGKKAKSPAAEFRGEDSHNSECPAAGAEGQKRG
                     PEIKRVAIPETEGARSRKTRKRAPKRKK"
     gene            5106..5177
                     /locus_tag="PKK37_00235"
     tRNA            5106..5177
                     /locus_tag="PKK37_00235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5138..5140,aa:Thr,seq:tgt)
     gene            5265..5732
                     /locus_tag="PKK37_00240"
     CDS             5265..5732
                     /locus_tag="PKK37_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80846.1"
                     /translation="MAGKTGKKNTKIQGSSREDQFVVLLENMRDEIKLVAEGNLMLQE
                     SMERGFKEIRKEFKEEINDVRIDMKMEINNVKAEISDMKAEISDMKTEMSDMKTEMSD
                     MKTEMSDMKTEMRMGFKGMMAESVVIDRKMEKKAEKKDLLILEKRVAKLESVR"
     gene            5861..6922
                     /gene="pheS"
                     /locus_tag="PKK37_00245"
     CDS             5861..6922
                     /gene="pheS"
                     /locus_tag="PKK37_00245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNR80847.1"
                     /translation="MKKDAMQDSDLEKIKNEASQEVDNAGNQAELLFVRKKYLDKDGV
                     LGEFFKLLGELPKEARGRMGVAANEIKKELTAAVEKKLAELGKTAVDNEGKRDFFDPT
                     LPGICPKVGHLHPFTLLEREITEIFGTMGFEIMESREIETEWYNFDALNVPADHPARD
                     AFDTLFLEDGLCLRPHTSPGQARYMESHQPPLRIIIPGRCFRRDAVDASHMPNFYQVE
                     GLMVDKDVSAANFKAVIGEFFKRFFHRSEGAAAENTDNIRLRPSFFPFTEPSFEVDLR
                     CVNCAGKGCSACKGTGWMEVMGAGMVHPNVLKNSGVNPDLWRGFAFGAGLDRLAMIKY
                     KINDIRLFYGGDQRFLNQF"
     assembly_gap    6926..7025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7026..9059
                     /gene="pheT"
                     /locus_tag="PKK37_00250"
     CDS             <7026..9059
                     /gene="pheT"
                     /locus_tag="PKK37_00250"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNR80848.1"
                     /translation="YEWLQSFFNKKLPAPRELGDLILARGFEVEKIGTLGKDWILDVN
                     ILSNRPDCLSHLGMVREIGAFLGIKPNFIKFKADKKSGQAAAKSIIAVEVRYPEACPR
                     YAAMAVAGTKIKSSPPWMVRRLEACGLRAINSVVDATNYAMLETGQPLHAFDWDKLAA
                     GRGKAKKIIVRAAQAQETFKTLDGKNFNLQPWMAVIADDGGALALAGIKGGKRAEIDG
                     KTETVILESANFDAKTIRRTSRTLGLSTDASYRFEHNIGSHGVLFAAHRAAELIVSVG
                     GGRIFAGAVDKRQNPETVKKIILNLPLAEKLLGTEIQTAVAKKNLEALGFAVRRGGRE
                     DLEIVPPPERGDVAVWQDIVEEIGRVGGYEKIAPQPPCAEIILPRQNRFWRAKSAAKD
                     LMAAAGYSEVRNHSFISEKDCRNFGINEEKLLEIKNPVNEDLRFLRPSLLMNLLKNFC
                     ANREFDSLRQFEIGKIFEKNRRDEPAMLAGASRKDSFSDAKGALEFLAARLRVKDFSV
                     VLLGQDKDAAAGFFDSAQSAAVMAGKKQVGFLGRLRREIADNFGIEPPVIFEIDFTRL
                     AQNIAEKIEYQPILAHPEAVRDISVDVPPQTLSADVANAVKKADKLKLIEMTEILGKP
                     YVSPDGRSKNILFRFHLRSAQKTLDGKDIDAWQAGAIAAIEKNPEWHVKKFKN"
     gene            9174..11099
                     /gene="gyrB"
                     /locus_tag="PKK37_00255"
     CDS             9174..11099
                     /gene="gyrB"
                     /locus_tag="PKK37_00255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNR80849.1"
                     /translation="MAKDDKNKNNGSYSAKDISVLEGLEPVRKRPGMYIGSTGLDGLH
                     HLIWEVVDNSLDEAMAGYCKNIDVILLPDNRIQVKDDGRGIPVDIHPQKKVSALQIVL
                     TTLHAGGKFENKAYQVSGGLHGVGVSVVCALSTWSKAEVCRDGGLYVQEYDRGKPRAA
                     VKKIGKCDEHGTTITFDPDPQIFKEIRFDPKKIITHLRQQAYLTKGVKINVINKSITP
                     EMAYSFYFEGGLYSYVKYLTRGKNALHQNVFYASGEKNGVAVEAAFIYTDEFEGMEQG
                     FANNIVTNEGGTHLTGFRAATTRVFNDYIRKNNILKANDDNLAGEDLREGLTAVVSIK
                     IAEPQFEGQTKGKLGNPEAKSAVETVVSEALTDFLERNPADARAILEKCILSHKARKA
                     AKAARETVLRKGILDGLSLPGKLADCSSRRPEDSEIYIVEGNSAGGSAKQARDRRFQA
                     ILPLRGKILNVERARLDKMLESKEIKSLIIALGTAVADDFNLEKLRYHHIVIMADADT
                     DGGHIRTLLLTLFFRYFKPIIEKGYLYVAQPPLYKMAAGKEVRYAYTEDEKESILKTF
                     NKPGLNLQRYKGLGEMNAEELWETTMNPRNRMLLQVQVEDAQEADATFDMLMGDEVLP
                     RKKFIQTYAKTVKNLDI"
     gene            11196..11480
                     /locus_tag="PKK37_00260"
     CDS             11196..11480
                     /locus_tag="PKK37_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR80850.1"
                     /translation="MEIEKVIKKKCTRIFKKYGVKKAAVFGSYARGEQKKNSDIDLLV
                     DVPDKIGLLGMVALKLELEAALKRKVDLVEYECIKPIIKDSVLSAQIPVL"
     gene            11477..11836
                     /locus_tag="PKK37_00265"
     CDS             11477..11836
                     /locus_tag="PKK37_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR80851.1"
                     /translation="MKRDQQLFITDIYDSIILIEKYLGKLSFEEFSQNSALLDAVVMR
                     FAIIGEAAKNVSRNFKAEHKDIPWKYMCGFRDVIVHEYFGINAKKTWQTAKEDLPKLR
                     SQIKTVLDEMGKDKLFK"
     gene            12070..12270
                     /gene="secE"
                     /locus_tag="PKK37_00270"
     CDS             12070..12270
                     /gene="secE"
                     /locus_tag="PKK37_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNR80852.1"
                     /translation="MDIIQSISKFLKESRAEIKKVNWLARPQLISYTLMVLGFMLATG
                     VFLGSLDWGFAFLLRKFVLGAE"
     gene            12319..12864
                     /gene="nusG"
                     /locus_tag="PKK37_00275"
     CDS             12319..12864
                     /gene="nusG"
                     /locus_tag="PKK37_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNR80853.1"
                     /translation="MAKQQIMSEKSWYVIHTYSGYEEAVAKNLKQRIESLGMEDKIFN
                     VIVPKEKKIKIKEGKRKTVEEKIYPGYILVEMVVTDDSWYVVRNTPNVTGFVGAGTTP
                     IPVSPEEIADLKRRMEMSEPEFNIEVAVGELVKITDGPFKDSEGKVSEIDRERGKVKV
                     LVSMFGRDTPVELDSLQIKKV"
     gene            13016..13438
                     /gene="rplK"
                     /locus_tag="PKK37_00280"
     CDS             13016..13438
                     /gene="rplK"
                     /locus_tag="PKK37_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNR80854.1"
                     /translation="MAKKVKSIVKLQVQAGKATPAPPIGPSLAQHGINLGEFVKQFND
                     LSRPMMGYKVGVEVTVYEDRSFSLTLKQPPASELLKKAAGIEKGSAQPNKKIVAKISK
                     AQLKKIAEQKMPDLNAPTAAAAEKIIAGTAKNMGIAVE"
     gene            13617..13913
                     /locus_tag="PKK37_00285"
     CDS             13617..13913
                     /locus_tag="PKK37_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR80855.1"
                     /translation="MKKVDFINSKNRDKLNAVFAEYGVKKAAVFGSVARGEQTNKSDI
                     DILVQFRGRKSLLDLVGLKFDLEKIFKRKVDVNTYDAIHPLLRDSIMEDQVCIF"
     gene            13919..14299
                     /locus_tag="PKK37_00290"
     CDS             13919..14299
                     /locus_tag="PKK37_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR80856.1"
                     /translation="MPSKKKKDPKFYLEHILQAADDVAEFIAGYDKKEFAADKKTNGA
                     VIHHLMIIGEAVKNLPMEFRKKYPDIPWRKIAGTRDQLIHEYFGVDLALAWIMATDNV
                     PDLKEKITQIMKDNKSSRQNKLLR"
     gene            14747..15328
                     /gene="dcd"
                     /locus_tag="PKK37_00295"
     CDS             14747..15328
                     /gene="dcd"
                     /locus_tag="PKK37_00295"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNR80857.1"
                     /translation="MILSDNDIKEYIAAGKIKIDPLPDFESCLGPCSLDLRLGNNFKM
                     FRPSNYNFIDVKNNYNGELMEEVIVGDDMPFIIQPREFVIAVTKETITLTPDLMGRLD
                     GRSSLGRLGLVVHSTAARFDPGWTGKAVMEFGNLGIRPIILYAGMRICAMTFETLSSP
                     AGVPYLSQKDHKYAGQSGAVASKIAEEENISKK"
     gene            15441..17042
                     /locus_tag="PKK37_00300"
     CDS             15441..17042
                     /locus_tag="PKK37_00300"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNR80858.1"
                     /translation="MISTKDIKLKFLGITPVWNDQTGSLSPQQLVALSGLLTYSGKSI
                     GAILEELRREGKDVDKKVAAILRQSSLKGHASMATTPALSFSYEASKFIDSALTGIVF
                     ASAIMASGRRTDTVPEDIVYPQAIAKDLRAREIYKIASEKNIETLNRLLAEGVGKDEA
                     SKILQYGIYGTGIIQFSVESLATLAREYDAEKEWMPEDVGFLIGAIEKELKGAGIDLL
                     YATRLAAPRNNYPYPNIFKDPEKTNLARAAADLAGGDEFKIITADFVLSAGLEQRLKS
                     LEQKIAAAVQSKENLMANWQEILSERQQICRDYGPSASIKIFSSSAWRVWGDKKRHRT
                     VPMAVDSVYESVRRAAEVFSARDAEIRTKQLGHREIDRINRVFSIPPAVLANKEFLYA
                     YLDAARSSFAAYQELLKIGIAPRDAVFAVPRGLRIDIVQDYNLYNLIAGYYPLRICST
                     AEEELRRASIKEVIAIKNLLQERKMEWLARHIVPKCHCAGFCLERDCCGVIKSLTKDY
                     DGDFHRSMHADLEKKFQTISNEIND"
     gene            17035..17643
                     /gene="tmk"
                     /locus_tag="PKK37_00305"
     CDS             17035..17643
                     /gene="tmk"
                     /locus_tag="PKK37_00305"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNR80859.1"
                     /translation="MIKNHHPGKFIVLEGLDGAGKSTHAKLAADFLRKSEGGVLLTQE
                     PSFLPAGDLSRRRLAGDWDCPPESLQLLFAADRADHLAKEILPALENGAAVIADRYFL
                     SSLAYGAVDVEMEWLAQINSRFLAPDLTIYLDVPPEVCAQRIAANGKSAELFEKVEIL
                     EIVRHNYKKAMEMLKGKMKIVLLDGNRNKEEVFGDIINKINL"
     gene            17640..>17886
                     /locus_tag="PKK37_00310"
     CDS             17640..>17886
                     /locus_tag="PKK37_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HNR80860.1"
                     /translation="MKQSSNKTVKKLPIAFRIDQTNIDPKASLSDIKKTCAEVKKYGF
                     RGICVNPQWVAAAKEELKDFPAKVVCLVDAPIGDSTHE"
BASE COUNT         4921 a         3607 c         4785 g         4473 t
ORIGIN      
        1 agtaactctt cagtttttga ttatgctttg gccggtttgg cttatttttg gaattgttat
       61 tttcttaaaa tttttattcg tcattttgat acccgaatgg cttgaacgtc gccggaatca
      121 caaacggttt gttgagggcg aggaatggag gtcggatcgg gatttgattt attggctacg
      181 aggaatgaag ccggatgaat ttgaaaatta tatcgccgat cttttttcgc gtttggggtt
      241 taaaaccgaa gttgtcggcg gatcgcatga cggcggcatt gatgttgttg cggaacaaaa
      301 cggaatcaag cattatatcc aatgcaagaa attcataaat cacgaggttg gtgttggcgc
      361 ggttcgcgat ttttacggtg cggttgccga tcatttggcg cagggaaaag gatattttat
      421 caccaccaat aagtttacac ttgaagcgga aaaattcgcc caaggcaagc cggttgagct
      481 tattgaccaa ttcaggctgg ttgaatatat cagattggca aaaaaagata tttctgatga
      541 tagaaaacaa aaaatctgtt caaagtgcgg cggcaatctt gttgaacgcg cgggcaaata
      601 cggaaaattt tttggttgcg aaaattatcc aaaatgcaat cacaccgaaa aattttgaaa
      661 cgatacggaa aaacaaaaac cattctgcgc gatgatttcg ggattgaggt taattttgcg
      721 ggcaaatgaa gtccaaaatc gggcaagtcg caagatattt actaaaaagc ttattttgct
      781 atgattaagc taatagattg tttggcgcgc gattaataat caattttatg gcggaaaaca
      841 aattatcgga agatcaaata ttatccataa ttaaaggaga gcgggaaaaa ctgtccaatc
      901 cttacggcga tcaaacggat ttggtggttg attttgcggt tggattgtac gagcgcaaag
      961 agattgaccg cgtggttttc aagacattga tatcgctggc gatattgaaa ttaactcaaa
     1021 aaaccgttgc cgatttgatg gataatttga taaaaaggaa tttgatttcg aaaaaatccg
     1081 gcagcgattc cgatgaattt gttttgttga attatttcaa aaaaaattat ggataaagta
     1141 attcaatcgt ccgatacgga tgaatcgcgg caaagcggca gggaaagcgg taaaacgcgc
     1201 gttgtttccg aaagtttgca gatgcttatg gccagtttgg gcggatcgca agatctgccc
     1261 gttaccgaag agcatatatc cgagatgctt tcgcaaagga ggcaagttaa tgagttcgtt
     1321 aatgagggca agaaaagaga ttcatatgat cagaggtatt gcttgacgat agggttgttg
     1381 tttatagtgg tgatttccgc attgataatt ttcacgcatc ccgaatattt cacgcaagtg
     1441 ctttcattga taatcggcgg gtttggcggg tttggtcttg gaagagcgtc taaaaaagat
     1501 tgaagcgaga tctgtcgccg accaatgaaa cggtcgtaat ccgttgaaca aagcccgttt
     1561 atagcggggt tttgtccagt tcgtcttgga gggtttggga aatgtcggcc aggccgccgt
     1621 ccaagatttt ttcaatgccg tgccaatttt tcttgatgcg atggtcggtc acgcggtcct
     1681 gggggaaatt gtaagtgcgg attttttcgc aacgctcgcc ggcgccgatc tgctcttttc
     1741 tggcgccgcc gaaagccgat tcctcctcct gccgtttttt ctcctgcagg cgatattcca
     1801 aaagcgccat cgcgttttcg cggttttgca gttggttgcg ctcggtttgg gaaaacaccg
     1861 ataatcccgt gggaatgtgg gtgatgcgca ctgccgattc gcgcttgttg acgaattgcc
     1921 cgccgggacc cgaagaacgg caaaaatcaa cgcggatttc gtcggagcgg atggagatgt
     1981 catttttctt gggcttgggc agaacggcca ccgtggcggt ggaggtgtgt atccgtccgc
     2041 tcttttcggt ttccgggatt ctttggacgc ggtgcacgcc gccttcgtgg cgcatcgccg
     2101 gccagacgcc ccggcccttc aattcaaaaa tgatttcctt gtagccgccg atttcggtct
     2161 ggttttggtc cagaactttt tgtttccatt cctgttcctg ggcgaagcgg gaatacatcc
     2221 gatagaggtt ggccgcgaac aacgccgatt cctggccgcc cgcgccggca cggatttcca
     2281 ccacgattgc tcccggaccg acggatttgc cctcgtcttt gagcagatcc cgcaattcgt
     2341 tttcaagctc ggttttccgg attttcattt gttcggtttc cacttccgcc agcgatacca
     2401 gctcggggtc ctcttcggcg gcgatgatca agcggttttc ttcaattttg ttggcgattt
     2461 ccttgatctc gttctctttt tcaatgattt tttccaactg aatcttcctt ttgttgagtt
     2521 cttcaaattt ttcccaatcc gaaatcagcc cggggtcgcc cagggaaacg agtagtcggt
     2581 catattcttc ctgatgcgaa agagcctgcg gcattttttg tttgtttaac tgccggattg
     2641 ttccggaact ccgctcccgg gtggtttatt tatccgcgcc ggaatcaccc ggggcttcac
     2701 cccggcatag tttatttgct ctttttgccc gctttcagcg cgtccttttt ggcgactttt
     2761 ttcaaaaact tgtcaacgcg gcccatcgcg ccgatcaacc gggctttccc ggtgtaaaac
     2821 ggatgacagg cggcgcaagt ctcggtttca agatattcct ttgaggaacc gatttgatat
     2881 gtctggccgc aagaacattt tatctgcgca tttggataat atttgggatg aatgtctttt
     2941 ttcataattt cgcttttcac gacaacgcca tcatataata attccggaga atttgcaaga
     3001 accgcggatt tatgatatgg aataacttgt ccggacgctt ctcttttgcg gataatgccg
     3061 gcgtgcaacg gcgcaaaatt gattgcggac ggcgacggaa tgaaaatatc ataatcatac
     3121 taattttaca gtaacatata tggcggttaa actatcagtt gaagaaatga ccgaggccgg
     3181 attgcaattc ggccatcgaa agtccaagat tcatcccaaa gcgagggggt ttttactcgg
     3241 cgtgagaaac acgattttca tcattgactt ggaaaagacc aaggaaaaat tcgccgccgc
     3301 gctggatttt gcgcggagtc tggcgtctga aggcaagacg attgttttgg tgggtaccaa
     3361 accgcaagtg aagaaaatga tcaaagaaac ggcggcggcg tgcgaaatgc cttatgtcaa
     3421 cgaacgctgg ctggggggta ctttcaccaa ctttaagatc atcaaaaaac ggattgacca
     3481 ttataaggat ctggagaaaa aaacgcttgc cggagaattt gaaaaataca ccaaaaaaga
     3541 gcaggcgatg atccaaaaag agatcaaagt cttggaaaag aaactgggag gcatcagggc
     3601 gatggaaaaa attcccgaag cgatttttgt ggtggatatg gaggatgacc ggctggcggt
     3661 taaagaagct aagcaaaacg gcgtgaaagt aatcggtatt tccgatgtga atatcgatcc
     3721 gaatattgcc gactatccca ttatcgccaa cgacgactcc atgtcttcgg tgaggttcgt
     3781 tttagaaaga atgaaggagg cgattttgga aggcaaaggg atgttcgtgg cgtcagtttc
     3841 tgccggcgct tcggaaaaaa ataaaaattg agaacgaaaa agaaaaaagg gaaatttaaa
     3901 attggacagc cggcgttatt tgacaatttt tcattttcat tttttacttt ttaatttgaa
     3961 acatggttga cattgaactt atcaaacaat tgagagaaga aaccggcatt tccctcggcg
     4021 aatgcaaaaa agcgctggaa gagggcaaaa ccgtggaaaa agctaaagaa atcctcaaag
     4081 aatggggtaa ggatctggcg gccaaaaagg aagatcgcca aacgggacag ggcaaagtcg
     4141 cgtcctatat tcatgccaac ggcaagatcg gggtcttgat tgaattgcgc tgcgagacgg
     4201 attttgtggc caattccgcg gattttaaga gcttgtcgca cgaactttgc ctgcaggtcg
     4261 cggcgatggg gggcgaagcg gaaaacattc cgggccagcc gtggatcaag gattcatcaa
     4321 agaacgtcaa ggacgttatc agcgaaaccg ttgccaaggt cggcgaaaac attgttttga
     4381 aaaaagccgc gcgctttcaa atttagcccg ctttccgaaa tatggttgaa attatcgctt
     4441 ccattgcgct tatcggcgga attgccggcg cgggaagcat tatcgcccgg aaaatgccgc
     4501 aagccgtcaa gactctcgag gccgatcgcg ccgcctttcc ggccggtgtt ttttctgggc
     4561 ttaaaaattg gattgtgggc agaatcaagc gccgctcttc catgaaagat ttttcctgga
     4621 ttgatttttc gcaaaaacag ctgttgaaaa ttcgcgtggc ggtcttgaaa attgacaaca
     4681 aaatcaatga aatcgccgga aaattacgcc agcgcgcgga aaaaaaacgg caagaagaac
     4741 aaatcgccgg cgacaattat tggcaggatc tgaaaaatgt ggtcaaggcg ggtcaatccg
     4801 cgtccgggaa aaaagcaaag tctccggcgg cggaattccg gggcgaagat tcgcataatt
     4861 cggagtgtcc cgccgccggc gcggaggggc aaaaaagagg gccggaaata aaacgggtgg
     4921 cgataccgga aacggaaggc gcccgttcgc gaaaaacgag aaaacgtgcg ccaaaaagaa
     4981 aaaaatagca aacttctttt ttgcggcgaa cggcaaaccg cgagattcgt gttttttgta
     5041 gtagtggaag tcaaacctcc actaacctcc gctaacacta acattaactt tttttgctgt
     5101 tctttgccgg ggtagctcaa tggcagagca actgttttgt aaacagtagg ttgggggttc
     5161 aagtcctctc ctcggctcat tttgcgcttt tcgcgcacgg tttgccgggt tgtgttgcgc
     5221 aaaatattga gccgtgatac aatcaagtta taattccatt acgtatggca ggcaaaaccg
     5281 gcaaaaaaaa taccaaaatt caaggatctt cgcgagaaga tcagtttgtg gtcttgctgg
     5341 aaaatatgcg tgacgagatt aagttggtgg ctgaaggtaa tttgatgttg caagagagca
     5401 tggaaagagg attcaaggaa atccgcaaag aattcaaaga agagatcaat gatgtgagga
     5461 ttgatatgaa aatggaaata aataatgtga aggcagagat aagcgatatg aaggcagaga
     5521 taagcgatat gaagactgaa atgagcgata tgaaaactga aatgagcgat atgaagactg
     5581 aaatgagcga tatgaaaact gaaatgagaa tggggtttaa gggaatgatg gccgaatcgg
     5641 tggtgattga tagaaaaatg gagaaaaagg cggagaagaa agatttgttg atcttggaaa
     5701 aacgagtggc aaaactggaa tccgtccggt aggataaaag acgaaaaaac aaaaaattca
     5761 aagatcgccc tggctggcga tttttgattg caagcctctc ttttgatgcg ccgccggtgt
     5821 tgtttttttg tatcaaatgg agtagaatga agtatcgcgg atgaagaaag atgctatgca
     5881 agattcggat ttggaaaaaa taaaaaatga agcgagccaa gaggttgata acgccggcaa
     5941 tcaggccgag cttctttttg tccgcaaaaa atatctggac aaagacggag ttttgggtga
     6001 attttttaag ttattgggtg aactgccaaa agaggcgcgc ggcagaatgg gggtggccgc
     6061 caatgaaatc aaaaaggagc tgacagcggc ggttgagaaa aaattggctg aattgggaaa
     6121 aaccgcggtt gataatgagg gcaagcggga cttttttgat ccgactttgc ccggaatttg
     6181 tcccaaggtg ggacatttgc atccgttcac cctgctggag cgggaaatca ccgaaatttt
     6241 cggcacgatg ggatttgaaa taatggaaag ccgcgagatt gagaccgagt ggtataattt
     6301 tgacgcgttg aatgttccgg ccgatcatcc ggcgcgcgat gcgtttgaca ctctgttttt
     6361 ggaggatgga ttgtgtttgc ggccgcacac cagtccgggc caggcgcgtt atatggaaag
     6421 ccatcagccg ccactgcgga tcatcattcc cggaagatgt tttcggcgcg atgcggtgga
     6481 tgcgtcccat atgccgaatt tttaccaagt ggaaggattg atggtggaca aagatgtttc
     6541 cgcggccaat tttaaggcag tgattggtga atttttcaaa agattttttc accggagcga
     6601 aggcgccgcg gccgaaaata ccgacaacat tcgattgcgg ccgtcgtttt ttccgttcac
     6661 cgagccgtcg tttgaagttg atctgcggtg cgtgaattgc gcgggcaagg gatgttcggc
     6721 gtgcaaggga accggttgga tggaagtgat gggcgcgggc atggtgcacc ccaacgtgtt
     6781 gaaaaattcc ggggtcaatc ccgatttgtg gcggggattc gcgtttggcg ccgggctgga
     6841 ccgtttggcg atgattaaat acaaaatcaa tgacatccgg cttttttacg gcggcgacca
     6901 acgcttccta aatcaatttt aaaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnntacga gtggttgcaa tcatttttca acaaaaaact tcccgcgccc cgcgaattgg
     7081 gggacttgat tctggcgcgc ggttttgagg tggaaaaaat tggcactttg ggaaaagatt
     7141 ggattttgga cgttaacatt ttgtccaatc ggccggattg cttgtcgcat ttgggaatgg
     7201 tgcgggagat cggcgcgttc ttgggaataa agccgaattt tatcaaattc aaagcggaca
     7261 aaaagtcagg ccaggccgcc gccaaatcaa tcatcgccgt tgaggtgcgc tatcccgaag
     7321 cgtgcccgcg ttacgcggcg atggcggtgg cgggaacaaa aatcaaatcg tctccgccat
     7381 ggatggttcg ccggctggag gcttgcggtt tgcgcgcgat caacagcgtg gtggatgcta
     7441 cgaactacgc gatgctggaa accggccagc ccctgcacgc ttttgactgg gacaaattgg
     7501 ccgccggccg cggcaaagcc aaaaaaatta tcgtgcgggc ggcgcaagcg caggaaactt
     7561 ttaaaacttt ggatggcaag aattttaatc tgcaaccgtg gatggcggtc atcgccgacg
     7621 atggcggggc gctggcgctg gcgggaatca aaggcggcaa gcgggccgaa atcgacggca
     7681 aaaccgaaac cgtcattttg gaatcggcga attttgatgc caaaaccatt cgccgcactt
     7741 cgcgaacatt gggattgagc acggacgctt cctatcgttt tgagcacaat atcggctctc
     7801 acggcgtttt gtttgccgca caccgcgcgg cggaattgat tgtttccgtc ggcggcgggc
     7861 ggatttttgc cggtgcggtt gacaagcgcc aaaatccgga gacggtcaag aaaatcatcc
     7921 ttaacctgcc gctggccgaa aaattactgg gtacggaaat ccagacggca gtagccaaaa
     7981 agaatttgga ggcgctggga tttgcggttc gccgcggcgg ccgcgaagat ttggaaatcg
     8041 tcccgccgcc cgaacgcggc gatgtcgctg tttggcagga tattgttgaa gagatcggac
     8101 gtgttggcgg ttatgaaaaa atcgcgccgc aaccgccttg cgccgaaatc attttgccgc
     8161 gccaaaatcg tttttggcgg gcgaaaagtg cggcaaaaga cctgatggct gcggcggggt
     8221 attcggaagt ccgcaatcac tctttcattt cggaaaaaga ttgccggaat tttggcatca
     8281 acgaagaaaa acttttggaa atcaaaaatc cggtcaatga agatttgcga tttttgcggc
     8341 cgtcgctttt gatgaatttg ctgaaaaatt tttgcgctaa ccgcgaattt gattcactgc
     8401 gccagtttga gattggcaaa atttttgaaa aaaaccggcg cgacgaaccg gcaatgcttg
     8461 ccggcgcctc gcgcaaagat tcgttttctg acgctaaagg cgcattggaa tttttggcgg
     8521 cgcgcttgcg ggttaaagat ttttcggtgg ttttgctggg gcaagataaa gatgcggcgg
     8581 ccgggttttt tgattcggcg caatccgcgg cggttatggc cggcaaaaaa caagtcggtt
     8641 ttttggggcg gttgcgccgc gaaatcgctg ataattttgg cattgaaccg ccggtgattt
     8701 ttgaaattga ttttacgcgc ttggcgcaaa atatcgccga aaagattgaa tatcagccga
     8761 ttttggcgca tcccgaagcg gtgcgcgaca tttctgtgga tgtcccgccg caaaccctgt
     8821 ccgcggacgt ggcgaacgcc gttaagaaag ccgacaaatt aaaattgatt gaaatgaccg
     8881 aaatcctcgg aaagccctat gtctccccgg acggaagatc aaaaaacatt ctttttcggt
     8941 tccatttgcg gtcggcccaa aaaacgcttg acggcaagga tattgatgcc tggcaagcgg
     9001 gggcgattgc ggcgattgaa aaaaatcccg aatggcacgt gaaaaaattt aaaaattaaa
     9061 aactaaaatt gaaaaacgac aattaaaaat ctaaaaccga aaatgcattt taatagtttt
     9121 tagttttaaa ttttcatttt tattttttca ttttcagttt ttaatttgaa actatggcga
     9181 aagacgacaa aaacaaaaat aacggatcat acagcgctaa ggatatttcg gtgctggaag
     9241 ggctggaacc ggtgcgcaag cggccgggaa tgtatatcgg ctccaccggt ttggacggtt
     9301 tgcaccattt gatttgggaa gtggtggaca actctttgga cgaggcaatg gccggctatt
     9361 gcaagaacat tgacgtgatt ttactgcccg acaaccgcat tcaggtgaaa gacgacggcc
     9421 ggggcattcc ggtggatatt catcctcaaa aaaaggtttc agcgctgcaa attgttttaa
     9481 ccaccttgca cgcgggaggc aaatttgaaa acaaggccta tcaggtttcc ggcgggttgc
     9541 acggcgtggg ggtgtcggtg gtctgcgcgc tgtccacgtg gtccaaggcc gaagtgtgcc
     9601 gcgacggggg gctttacgtg caggaatatg accgcggcaa accgcgcgcc gcggttaaaa
     9661 aaattggaaa atgcgacgag cacggaacaa cgataacatt tgacccggat ccgcaaattt
     9721 tcaaagaaat ccgctttgat cccaaaaaaa tcatcactca tttgcgccag caagcttatt
     9781 tgacaaaggg ggtgaaaata aatgttatca acaaatcgat tacgccggaa atggcctact
     9841 cgttttattt tgaaggggga ttgtattcct acgtgaaata cctgacgcgc ggcaaaaatg
     9901 ccctgcacca aaacgttttt tacgccagcg gcgagaagaa cggcgtggcg gtggaggcgg
     9961 cgtttatcta taccgatgaa tttgaaggaa tggagcaagg ttttgccaac aatatcgtca
    10021 ccaacgaggg cggcacgcat ttgacgggtt ttcgcgcggc cacgactcgg gtttttaacg
    10081 attatatccg caagaacaac attttgaaag ccaatgacga caatctggcc ggagaggatc
    10141 tgcgcgaagg tttgaccgcg gtggtttcca tcaaaatcgc cgaaccgcag tttgaaggcc
    10201 agaccaaggg aaaattgggc aatcccgaag ccaaaagcgc ggtggaaacg gtagtatcgg
    10261 aggcgttgac cgatttcttg gagcggaatc ccgccgacgc gcgggcgatt ctggaaaagt
    10321 gtattttgtc gcacaaagcg cgcaaagccg ccaaggccgc gcgtgaaacg gtgttgcgca
    10381 aggggatttt ggacggtttg tctttgcccg gcaaattggc ggattgttct tcgcgccgtc
    10441 ccgaagattc ggaaatctat atcgtggaag ggaattccgc cggcggttcg gcaaagcaag
    10501 cgcgcgatcg ccgttttcag gcgattctgc ccttgcgcgg caagattttg aatgtggaac
    10561 gcgcccgatt ggacaagatg ctggaatcaa aggaaatcaa gtcgttgatt atcgcgctgg
    10621 gcaccgcggt ggccgatgat ttcaatctgg aaaaactgcg ctatcatcac attgtgatta
    10681 tggccgatgc cgataccgac ggcggccata ttcgcacgct tctgctgacg ctctttttcc
    10741 gttatttcaa gccgatcatt gaaaagggtt atctttatgt tgctcagccg ccgctctata
    10801 aaatggccgc gggcaaggag gtgcgttatg cctataccga agatgaaaaa gaaagtattc
    10861 tgaaaacttt caacaagccg ggattgaatc tgcaacggta taaagggttg ggggaaatga
    10921 atgccgagga attgtgggaa accacgatga acccgcgcaa ccggatgtta ctgcaagtgc
    10981 aagttgaaga tgcccaggaa gccgacgcca cttttgatat gctgatgggc gatgaggttc
    11041 tgccgcgcaa aaaattcatt caaacctacg ccaaaaccgt gaaaaacctg gacatataat
    11101 gaatatgcca aaactcagaa cgcggaaccc agaaccaaat ctcaaaatct aaaaggtctg
    11161 cttgtggatt ggggtatttg tgttaagatt gaggcatgga gatagaaaaa gttattaaaa
    11221 aaaaatgcac gagaattttt aaaaaatacg gcgtgaagaa ggccgcggtt tttggttctt
    11281 atgcccgcgg agagcaaaaa aagaacagcg atattgatct gctggtggat gttcccgata
    11341 aaatcggttt gttgggaatg gtggcgttga agctggaatt ggaagcggca ctaaagcgaa
    11401 aggttgattt ggtggagtat gaatgcatta aaccgataat caaagattcg gttttaagcg
    11461 cgcaaattcc ggtgctatga aaagagatca gcaattattt ataaccgata tttacgacag
    11521 cattattttg attgaaaaat atcttgggaa attatctttt gaagaatttt cgcagaattc
    11581 ggctttgctt gacgcggtag taatgagatt tgcgattatc ggcgaggcgg caaaaaatgt
    11641 atcaagaaat ttcaaggccg agcacaaaga tattccttgg aaatatatgt gcggcttcag
    11701 ggatgtgatt gttcatgaat attttggcat taacgccaaa aaaacatggc aaaccgcgaa
    11761 agaggatttg cccaagttaa gatcgcaaat caaaacggtc ttggatgaga tgggtaaaga
    11821 taaattgttt aaataaccga tataacgtaa ccgctcactg gaggtattgc attttaagga
    11881 tccatccctc gcgcttatcc cgaggtaatc cgggggataa cgagagcggt tagactctgg
    11941 caaaagaatt ttaaatccag tgagtggttg cattttttaa ttttgacatt tttcggggtt
    12001 tttggtataa ggaagacatg aagcccgcgg ggctttttta ggagcggctt accgttcaat
    12061 ataaatctaa tggatatcat tcaaagcatt tccaaattct tgaaagaatc gcgggcggag
    12121 atcaaaaagg tcaattggct ggcgcggccg caattaatca gctacactct gatggtgctc
    12181 ggctttatgc tggccaccgg cgttttcttg ggatcgctgg attggggatt tgcctttctc
    12241 ttgagaaaat ttgttttggg cgcggaatga aaattttcgc ggggcaatcg tttttgtttt
    12301 taaaattcgg tttaaactat ggcaaaacaa caaataatgt ccgaaaagag ctggtatgtg
    12361 attcatacct attcgggcta tgaagaagcg gtcgccaaaa atctcaaaca aaggattgaa
    12421 tcgctgggga tggaagacaa gattttcaac gtgatcgttc ccaaagagaa aaaaataaaa
    12481 atcaaggaag gcaaacgcaa gaccgtggaa gagaaaattt atcccggcta cattctggtg
    12541 gaaatggtcg tgaccgacga ttcctggtat gtggtgagaa acactcccaa tgtgacgggg
    12601 tttgtgggtg cgggaacgac gccgattccg gtgtcgcccg aagagattgc cgatttgaag
    12661 agacggatgg aaatgtccga gcccgagttc aatattgagg tggcggtggg ggaattggta
    12721 aaaatcaccg acgggccttt taaggattcg gaaggcaagg tgtccgagat tgatcgcgag
    12781 cgcggcaagg tgaaggtgct ggtgtcaatg tttggccgcg acacgccggt ggaactggac
    12841 tcattgcaaa tcaagaaagt ttaaaattta aaaatcaaag ttaaaaatga cagtgtaaag
    12901 ttgaaaatta aaaatacgtc agaaggcatt ttaacatttt cccgccggag gcgggtgcgc
    12961 ctatggcgca aattgtaatt ttgatttttg atttttacat tttcaattta ttatcgtggc
    13021 taaaaaagtt aaatcaatcg tgaaacttca ggtgcaggcg ggcaaagcga ctcccgcgcc
    13081 gccgatcggg ccgtctttgg cccagcacgg catcaatctg ggggagtttg tcaagcaatt
    13141 caatgatctt tcccgcccga tgatgggata caaagtcggc gtggaagtga cggtctatga
    13201 agaccgcagt ttttcgttga cactgaaaca accgccggcc agcgaattgc tgaaaaaagc
    13261 cgcggggatt gaaaaaggat cggcgcaacc caataaaaaa atcgtcgcca agatcagcaa
    13321 ggcgcaactc aaaaaaatcg ccgagcagaa aatgccggac ctgaacgctc cgactgccgc
    13381 cgccgccgaa aaaatcattg ccggcaccgc caagaatatg gggattgcgg tggagtaacc
    13441 cgaatttgag atattgtgtt tgattttata tgtgggggca ccccttgtgg gtgccctttt
    13501 ggataattgc ctcaaaataa aatacgggcg ggcacaaaac ctgcccctac gaaaataccg
    13561 ctgaacaaaa agatgttatt atttgatatt ttggcaaggt ttgataggat ggaagtatga
    13621 aaaaggtgga ttttatcaat tcaaagaaca gggacaagtt gaacgcggtt tttgcggaat
    13681 atggtgttaa aaaagccgcg gttttcggat cggtcgcgcg cggagaacaa acgaataaaa
    13741 gcgacattga tattctggtc caatttcgtg gcagaaagtc attgcttgac ttggttggct
    13801 tgaagtttga tttggaaaaa atttttaaaa ggaaagtgga tgttaatact tatgatgcca
    13861 tacatccgtt attgcgagat tcaattatgg aagatcaggt gtgtattttt taaaaaatat
    13921 gccatcaaaa aagaaaaaag atccaaaatt ttatttggag catattttgc aggcggctga
    13981 tgatgttgcc gaatttattg ccggttatga taaaaaagaa tttgccgcgg ataaaaaaac
    14041 gaacggagcg gtcattcatc atttaatgat cattggcgaa gcggtgaaaa atctgccgat
    14101 ggaatttcgc aaaaaatatc ctgatattcc ttggcggaaa atcgcgggca cgcgagatca
    14161 attgattcat gaatatttcg gagtggattt ggctttagcg tggattatgg caacagacaa
    14221 tgtgcccgat ttgaaagaaa agattaccca aataatgaaa gacaataaat ccagccggca
    14281 aaataaatta ttgcgataaa tttgaccttg attttttgtg taggggcacc ccttgtgggt
    14341 gcccttttag taacgaaata cgggcggcca taaaaaccgc cccgcaagac atttttttat
    14401 gagacataaa acttattttc acggttagtt ttgttttttg tcaagggtgg aaagagggag
    14461 gggaaagggg tataatcaaa aatatcaaag ctcaaagttc aaatttcaaa tatagaaatc
    14521 acattcacga atcggactat ttattataaa atttcgtgaa tgtgctttct atgattccca
    14581 tcaccagtcc gatttcatag tgtgatgtga ataaatgcaa tgccaaaaat tcaatcccaa
    14641 tttgaaattg tatttttgtt ttgatatttg acatttgggt tttggtatta atttttgcga
    14701 gggtctaacc ttcgcgatta cacgagggac agaccctcgc aaaattatga ttctatcgga
    14761 taatgacatt aaagaataca ttgccgccgg caagatcaag attgatccgt tgccggattt
    14821 tgagagttgt ctggggccgt gttcgctgga tttgcggctg ggcaataatt tcaagatgtt
    14881 tcggccgtca aactacaact tcattgatgt gaagaacaac tataacggcg aattgatgga
    14941 agaagtgatc gtgggcgacg atatgccgtt tatcatccag ccgcgcgaat ttgtgatcgc
    15001 ggtgaccaaa gaaacgatca ccctgacgcc ggatttgatg ggacggttgg acggacggtc
    15061 gtctctcgga cggctgggtt tggtggtgca cagcaccgcc gcgcggtttg acccggggtg
    15121 gacgggtaaa gcggtgatgg aattcggcaa tctgggcatc cggccgatca ttctttatgc
    15181 gggaatgcgg atatgcgcga tgacttttga aacgctctct tcgccggcgg gcgtgcccta
    15241 cctgagtcag aaagatcata aatacgccgg ccagagcggg gcggtggcat caaaaattgc
    15301 cgaagaagaa aatataagta aaaagtaaaa atgaaaaatt aaaaacgata attttaaact
    15361 aaaaattatt aaatatgaat tttcattttt aaattttagt tttcgttttt aacttttcat
    15421 tttcagtttt taaattagag atgatttcaa cgaaagatat taagctaaag tttttaggga
    15481 taacgccggt gtggaatgac caaactggta gtttgtcgcc tcaacaattg gtggcgctgt
    15541 cggggctttt gacttacagc ggcaaatcca tcggcgcgat tttggaagaa ttgcggcgcg
    15601 aaggcaagga tgttgacaaa aaagtcgccg cgatcttgcg ccagtcgtcg ctgaaagggc
    15661 acgcgtcaat ggcgacgacg ccggcattat cgttttcata cgaagccagc aaattcattg
    15721 attcggcgct cacggggatc gtgttcgcat cggcgattat ggcttcggga agaaggaccg
    15781 acaccgtccc tgaagatatc gtctatccgc aagcgatcgc caaagatttg cgggcgcggg
    15841 aaatttataa aatcgcctcc gaaaaaaata ttgaaacgct caatcgcctg ctggcagagg
    15901 gagtgggcaa ggacgaagcc agcaaaattt tgcaatatgg aatttatggc accggcatca
    15961 ttcagttttc ggtggaatcg ctggcgacat tggcgcgcga atatgacgcc gaaaaggaat
    16021 ggatgcccga agatgtcgga tttttgatcg gcgcgattga aaaagagttg aagggcgcgg
    16081 ggattgactt gctctatgcc acccggctgg cggcgccgcg caataattat ccctatccca
    16141 atattttcaa ggatcccgaa aaaaccaatc tggcgcgcgc ggcggcggac ttggcgggag
    16201 gagatgaatt caaaatcatt actgccgatt ttgttctttc ggcggggttg gagcaaaggc
    16261 tgaaaagctt ggagcaaaaa atcgccgcgg cggtgcaaag caaagagaat ttgatggcga
    16321 actggcagga aatcttgtcc gaacgccagc aaatttgccg cgattacggc ccgtcggcca
    16381 gcatcaagat tttttcttcg tcggcatggc gggtttgggg cgataaaaaa cggcatcgca
    16441 ccgtgcccat ggcggttgat tcggtttatg aaagcgttcg ccgcgccgcg gaagtctttt
    16501 cggcgcggga cgccgaaatc cgcaccaaac agcttggcca tcgggaaatt gaccggatta
    16561 accgcgtttt ttcaatcccg ccggcggtgc ttgccaacaa agaatttctc tatgcctatc
    16621 ttgacgcggc acggtcgtcg tttgccgctt atcaagagtt gctaaaaatc gggatcgcgc
    16681 cgcgggacgc ggtttttgcc gtgccgcggg ggttgaggat tgacatcgtg caggattata
    16741 acctttacaa tctcatcgcc ggatattatc cgttgcggat ttgttccacc gccgaagaag
    16801 agcttcgccg cgccagcatc aaggaagtta tagcaatcaa aaatcttttg caagagcgga
    16861 aaatggaatg gctggcgcgc catatcgtgc ccaagtgcca ctgcgccgga ttttgcctgg
    16921 agcgggattg ttgcggagtg atcaagagct tgaccaaaga ttatgacggc gattttcacc
    16981 gatcaatgca cgccgatctg gagaaaaaat ttcaaacgat atcaaacgaa atcaatgatt
    17041 aaaaatcatc atccggggaa attcatcgtt ttggaaggat tggacggcgc ggggaaatca
    17101 acccatgcga aattggcggc ggattttttg cggaagtccg aagggggcgt tttattgacg
    17161 caagagccga gttttttgcc ggcgggcgac ttgtcgcggc ggcggctggc gggcgattgg
    17221 gattgtccgc cggagagttt gcagttgttg tttgccgcgg accgtgccga tcatctggcg
    17281 aaagaaattt tgccggcgct ggaaaacggc gcggcggtca ttgccgaccg ctactttttg
    17341 tcgtctttgg cttatggcgc cgttgatgtt gaaatggaat ggctggcgca aatcaattcg
    17401 cggtttttgg cgccggatct gacgatttat ctggatgtgc cgccggaagt ttgcgcccaa
    17461 agaatcgccg ccaacggaaa atccgcggaa ttgtttgaaa aagttgaaat tttggaaatt
    17521 gtgaggcaca attacaaaaa ggcgatggaa atgctcaaag gaaagatgaa aatcgttttg
    17581 cttgacggca atcggaataa ggaagaagtg ttcggcgata tcatcaataa aataaattta
    17641 tgaaacaatc ttcaaataaa actgtcaaaa aactgccgat cgcgttccgg attgaccaaa
    17701 ccaacattga tcccaaggcg agcttgtcgg acattaaaaa aacttgcgcg gaagtgaaaa
    17761 aatacggctt tcgcggaatc tgcgtgaatc cccaatgggt agccgcggca aaggaagaat
    17821 tgaaagactt ccccgccaaa gtggtttgtc tggtggacgc accgattggc gacagcacgc
    17881 acgaag
//
LOCUS       DAOQWE010000003        16474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_3941_length_16474_cov_8.491747, whole genome shotgun sequence.
ACCESSION   DAOQWE010000003 DAOQWE010000000
VERSION     DAOQWE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 16474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16474
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3941_length_16474_cov_8.491747"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..465)
                     /locus_tag="PKK37_00315"
     CDS             complement(121..465)
                     /locus_tag="PKK37_00315"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNR80861.1"
                     /translation="MKFLVDENVKLRLAKMLLAAGHDVKLAKRGSSDKEIAVTAKKES
                     RIIITHDNDFALPNLFAANEYAGIILIKVMPPTFEKIATALKNIFCQMKTAKSFKGKL
                     IVLLDEKSFWIE"
     gene            complement(449..706)
                     /locus_tag="PKK37_00320"
     CDS             complement(449..706)
                     /locus_tag="PKK37_00320"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNR80862.1"
                     /translation="MASLSINKYIVADPEICHGKLTFRGTRVMVWQVLAMLAAGETVE
                     EILADFPSITKKHINAALSYAAQAAQRGSAQTIKKFDEVSC"
     gene            complement(840..1484)
                     /locus_tag="PKK37_00325"
     CDS             complement(840..1484)
                     /locus_tag="PKK37_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80863.1"
                     /translation="MKESLKRADDLYRQGKYQEAYDLAVPCDQKLDPEGCAEACRIRA
                     WAKYYIAIKGNPEEKIRNAGHAAGYASVVSISIDPKRRLSAYNVLPLALWISGNQSGA
                     WEASDEALQEFPEEPSAWNTRGILARWAKDYEQGVIIGDRVTDRSVARGDFLTAGHGQ
                     QNKGDALRALGRIGEAHTAYEEALKRYRQHEHSSGKSATPHIVNAEKKLAEVSK"
     gene            1750..2157
                     /locus_tag="PKK37_00330"
     CDS             1750..2157
                     /locus_tag="PKK37_00330"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNR80864.1"
                     /translation="MANQAQTPRWQQRLKDFEKSAARLKEAASKKEFSDLEKDGVIQR
                     FEFTFELAWKTLKDYLENQGFSGIASPKKALQKSFSMDLASDGNVWINMLEDRNRMSH
                     PYSRAASEKIFQNIKKYYVPAIDELAANLKKQI"
     gene            2162..2470
                     /locus_tag="PKK37_00335"
     CDS             2162..2470
                     /locus_tag="PKK37_00335"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR80865.1"
                     /translation="MSHGLTAKEIKLIKDVFKKNSAVERVFIHGSRARGDFKKTSDID
                     FAVKFFGNDKNLILQLHSELDDLPVIYEIDIIDEKEIGGKNFKDDYEKSKKEFYKKSK
                     "
     gene            complement(2535..3125)
                     /locus_tag="PKK37_00340"
     CDS             complement(2535..3125)
                     /locus_tag="PKK37_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNR80866.1"
                     /translation="MNDEERGMRNKNKEIILKGQKVAYCLKTSARTRNLRVVVKSGGE
                     VFASKPEFFPDFLVARFLRQKANWILGKIAESKSQKSLLPPARRREFLEHRPAARRLV
                     FDKIAELSVVYGFKYNRVAIRNQRTRWGSCSAKGNLNFNYRMVFLPDRLAGYIVAHEL
                     CHLKEFNHSPRFWALLAIAFPDQKNLRRELKNYHLS"
     gene            complement(3128..3457)
                     /locus_tag="PKK37_00345"
     CDS             complement(3128..3457)
                     /locus_tag="PKK37_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNR80867.1"
                     /translation="MEFSEYQRLARKTAIYAGAGKNFVYPALGLCGESGEVAEKIKKI
                     VRDRDNVVDDKCRAELEKELGDVLWYIANLAVELDLDLENIAQKNIEKLADRKERGVL
                     HGSGDNR"
     gene            complement(3536..3970)
                     /locus_tag="PKK37_00350"
     CDS             complement(3536..3970)
                     /locus_tag="PKK37_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNR80868.1"
                     /translation="MKLGSPIFQNNDGFPPAHTCDGAESSPPLEISGVPAAAESLALV
                     LRDPDAPGGIFIHWILFGIPPKTEKIDIGGIPPGARQGKTTNGKIGYVGPCPPSGTHR
                     YIFTLYALDTKMYLNDGAPIERFNAAISGHVLETAQLTAKYR"
     gene            complement(4001..5665)
                     /locus_tag="PKK37_00355"
     CDS             complement(4001..5665)
                     /locus_tag="PKK37_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR80869.1"
                     /translation="MTQEQAMEILKSGKNAFITGPAGSGKTFVLNRYIDWLKASGANV
                     AVTASTGIAATHLDGITIHSWTGMGVRDSLSKAEVKDLLKRGYLRRRFLRADVLVIDE
                     ISMLSRIQLDLADLIFREFKQVDAPFGGIQTVLCGDFFQLPPIARKNEPAGAKKAAQS
                     SLNFAEEDAPSEFSYHARSWKALDLKICYLDEQYRQSDQEFLRALNAIRSNSAGESVV
                     DYLQSRVDAKINLPVEPTKLYTHNVDVDVENNLELQKISGKEFRYKMELVGPAALAER
                     LQKGCLAPPVLRLKAGARVMFVKNNFEEGYVNGTLGVVQELEEGRILVRTARGAIDVG
                     PAAWHIAEEGKIKAEISQYPLRLAWAITIHKSQGMSLDAARVDLRQSFEPGMGYVALS
                     RVRSSAGLSLAGFNKISLQVNAEVLEFDKDLKRRSEISARQIAAKILPPRRFSPAKPA
                     KEKVDTVAATRELLNQKFSLAEIAEKRNLTAGTILAHCEKLKAKFPDFDFSGLAKNLP
                     PEKIKQMKTALKKSQNENGAYPLTPAMELLGGTATFEELRLARLLI"
     gene            5805..6506
                     /locus_tag="PKK37_00360"
     CDS             5805..6506
                     /locus_tag="PKK37_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80870.1"
                     /translation="MKKNIVIIAIILIAAIAGLAMMMNRKSGEGNKNGEEPAGKLSPL
                     NAAYLVETDMITLENGKAESINPYGAQKMTTEVDGQPVSGDLNSDGQNDWALVLKQQT
                     ENDPAIYYYAAIALVDEGKSLIVGSNAVPLGEGVKIEDIAIVNQAMRINYLERKIDGD
                     AVEEEPTVPVSKSYILDGAMLKELTQKRANAQAEAACTDNRGEWNMENQECSGISQEL
                     CDRFGGKFENELCKF"
     gene            complement(6577..6873)
                     /locus_tag="PKK37_00365"
     CDS             complement(6577..6873)
                     /locus_tag="PKK37_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80871.1"
                     /translation="MYGFAAKRFDRDIYKNRRWLRNADFALGFLTALLAWLLFFRVVW
                     WFAPMMVFAACMAGILAKIKRRYLIYGALSLVFFPLIAWATLMIAWAGRNEINS"
     gene            complement(6935..7738)
                     /gene="mutM"
                     /locus_tag="PKK37_00370"
     CDS             complement(6935..7738)
                     /gene="mutM"
                     /locus_tag="PKK37_00370"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNR80872.1"
                     /translation="MPELPEVETIRRQLEQKAVGEVINKIEILEPKMFIGDPKDVIGS
                     RITGVGRAAKVLRIRLANGAALLAHFKLNGQFFWRAAGDNTPARFTRAIFYFESGARL
                     LFNDSRKFGWIKAIADFKDDPGAAIEPFRPNFTLQNFSSVLGKSRKPIKLLLMDQEKI
                     GGIGNIYANESLFAARVSPFRPANSLDQKEIKKLFDSIVGVLEKAIDCRGSSGKDEWY
                     RQLDGSPGCYQDHFLVYQRNGQECFACGGKILRQTQSGRGTFFCPQCQK"
     gene            complement(7838..8503)
                     /locus_tag="PKK37_00375"
     CDS             complement(7838..8503)
                     /locus_tag="PKK37_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNR80873.1"
                     /translation="MKEGLRKMARVVLVAAALAASFAIFADTYVFWHGSQGIYENAAD
                     LPAADAVLVLGAAVYRGGRMSAILTDRAATALEVYHAGKAKKILISGDHSQNNYDEVN
                     IAKDFFLQNGVPAEDVFVDYAGFNTYDSAARAKEIFQAGSLIVATQEFHLPRALYLVR
                     HAGITSHGIKADRRPYNPGFYNTWRENGARVKAFWRVLFNDSPRFLGEAIPITGDGRA
                     SWD"
     gene            8713..9414
                     /locus_tag="PKK37_00380"
     CDS             8713..9414
                     /locus_tag="PKK37_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80874.1"
                     /translation="MFSKILQILSSAKNCSKCNGILLVITGPSGVGKDTAKELFLKRN
                     PGFHKIVTDTSRLPRDGEIKGVSHNFYTVEEFKKRIKEGKYLEYIEVRPQEYKGTSKE
                     SISAILSGENVIWQIDEYAMAHIKRILKENMPDIADCILQKTVTIYIAPEDWDQLREQ
                     YFRRDPNASEEKFLGKLARDREMWWQYHDRYDHVVINKRGKINETVSMIEKLVAQKAE
                     SLVDARLIAPAAIIR"
     gene            9463..10194
                     /locus_tag="PKK37_00385"
     CDS             9463..10194
                     /locus_tag="PKK37_00385"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNR80875.1"
                     /translation="MMDKTVKIAKYLAQAGVASRRKAEALVKQGLVSVNGRVSADPAV
                     RINPETNKILYRGKPLSPAQKVYYLLNKPAGYTCTAQDPHAEKLVGELVPAEPKVWSV
                     GRLDKMTSGLLILTNDGDLALSLTHPRYRVEKEYEITANLPLAENEIAAVQKGVLLED
                     GFIRPDRFEKIGGKRYRIVIHSGKKRVVRRIVEKIGKSVAQLKRTRVGSLKLQDLPEG
                     KWRRLSSAEIKKLAQRPATPTNAAD"
     gene            complement(10178..10984)
                     /locus_tag="PKK37_00390"
     CDS             complement(10178..10984)
                     /locus_tag="PKK37_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR80876.1"
                     /translation="MTEGYLENGVYWRHNGFWPGRETMIFVHGLSGSSSAWIPYEEKF
                     GKKFNLLFFDLRGHGKSVKKADGDYYVLKNISGDIAALAERFKLQKFILVGHSLGAIL
                     AMDFARRHKKKVSRLILLAPNYRANKTWRAKIVNFFLLFAGITEPMPFEERIGVHIDY
                     RRFFGTGDWDAGRMYADIRNTSLKVYCRCLREAKKFDGEKFIAGMDMPVLIVHGRKDT
                     IFPAADSARLAKKMPNARLEILPDANHILVLNNFRETGKIMEDFLFNPPR"
     gene            complement(11316..11669)
                     /locus_tag="PKK37_00395"
     CDS             complement(11316..11669)
                     /locus_tag="PKK37_00395"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HNR80877.1"
                     /translation="MVEIELIGDGGSQKVLALLDSGADYSLFDYDLAVAAGIDLSKSR
                     DDRLTGIGGETLGKRLDKVDIKVQDIVNAVKIPACFIKNFTAGALIGQEGFFDQYKIK
                     FEKDHDTFEIIPVRK"
     gene            complement(11741..11941)
                     /locus_tag="PKK37_00400"
     CDS             complement(11741..11941)
                     /locus_tag="PKK37_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80878.1"
                     /translation="MNTKNINFNLIKLAPYANQWVALSLDRKKVLASGKKVADVTSKL
                     KTKDAVLFKVFSPDTFYMPRAD"
     gene            12212..12661
                     /locus_tag="PKK37_00405"
     CDS             12212..12661
                     /locus_tag="PKK37_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNR80879.1"
                     /translation="MDAFTQIINNQPLVAGFLAWLIAQFIKMVLALKHGINRAAFRRF
                     WASGGFPSSHSSAVAALSAACGLVYGFGSPYFAIAAVFALIVIYDAFTLRREAGKHAE
                     ALNQIMEEVYLNKQIKIEHLKELLGHTRFEVFFGFLLGAVVAVLIAG"
     gene            complement(12704..13687)
                     /locus_tag="PKK37_00410"
     CDS             complement(12704..13687)
                     /locus_tag="PKK37_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNR80880.1"
                     /translation="MDNKLYLGVDIGSISTKAVAIDKGNNIFARSYLWTEGNPIGAVR
                     RVLDEIQRQIAGRGEVFAVGTTGSARELIGAVLNATIVKNEITAHAIGTLAFHPEVRT
                     ILEIGGQDSKIIILEDGVITDYAMNTLCAAGTGSFLSSQARRLNIPVEEFGARALKSK
                     NPTKIAGRCTVFAESDLVHKAQMGHPTEDIIAGLCNSIVYNYLNNLGKGKTINAPIVF
                     QGGVSKNVGVARAFENIIGEPIIVDEIGHLMGAMGIAILARDQEAERFFDFGVKDVEF
                     STAGVECGGCPNNCEVVCVFNDEGFIDGWGNRCERGIERAKAKLKEDGRLK"
     gene            complement(13680..14756)
                     /locus_tag="PKK37_00415"
     CDS             complement(13680..14756)
                     /locus_tag="PKK37_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80881.1"
                     /translation="MPEKKVSFPHMGDYYSAFKCLVELIGRPLVPPPITKKTIELGFK
                     HSPETVCIPFKYNLGNYIEALEAGADVICQAGGGCRFGYYAEVQEQILRRLGYEFEFF
                     KLNNKNSVIGFIKDFKRIAPHNTYPGIIHRLALCLARIHCIDAVSDCVRKNIGFEIHP
                     GEMEKTYRKFLEKIARTYSLGAVFSLKNECLRQIRQIDIDKPADCLRVGVVGELYMLM
                     EPFANFYLEKKLAQRKIEVHRFVTVSGILTEYFSGDRSVKKHLAAAHPYLTHHIGAHG
                     TETVARANSLIKKGFDGIIHLKPFGCMPEVSAMAALNNLARDRHAPILYFSFDSQTSE
                     AGLETRVEAFCDMLYMKQKKKKNG"
     gene            complement(14738..15739)
                     /locus_tag="PKK37_00420"
     CDS             complement(14738..15739)
                     /locus_tag="PKK37_00420"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNR80882.1"
                     /translation="MAVAKRGARWYVAIVENAAGDKKNKLRVGIPRALLYHKYGKLWE
                     VFFRELGVNVVLSPETNKEIIARGARLSIDESCLSVKIFVGHIDWLKDKADAVLVPHI
                     TCLHSGEEMCVKFMALVDTVRNVFPGIKVIEYSVNAKKLETEAGSMIRLGARLAGNPF
                     RAIAAYMKAKGAWEKSAREKKEAQAEKFAAARRGEKPLILIVAHSYTLEDNFLGRPII
                     DYLEKSGVCVLCSDRLDAARARKMSENISQTLYWTYHKELLGAAAYYKDSVDGMIFLT
                     TFPCGPDALAVNLCQSKMKGIPSIIVSLDELESRVGLQTRLESFVDVINFKKCRKKK"
     gene            15918..16304
                     /locus_tag="PKK37_00425"
     CDS             15918..16304
                     /locus_tag="PKK37_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80883.1"
                     /translation="MKLKKLNQNETIESANLKQQTETTFDTIKAEPDKAKKRQMLLDV
                     AKENPDVVEKMMEIAKDRALGLDYRERMIKQLGVESGERAAYIASKIKEMKSKEEKRE
                     YVKNLIEKKLLTDDVAKQMLELMKQK"
BASE COUNT         4419 a         4257 c         3673 g         4125 t
ORIGIN      
        1 ccgccattca tccccggggc aaagccccga ggtattctgg cgggtaaata aaaaaatccg
       61 gcggcgcaat tcggattttc acccgctcgc gccccggaaa atatttttaa atatatcccc
      121 ctattcaatc caaaatgatt tttcatcgag caaaactatc aatttgccct taaagctttt
      181 tgccgttttc atttgacaga aaatgttttt caatgccgtg gcgatttttt caaaagtcgg
      241 tggcataact tttatcaaga ttattcccgc atattcattg gcggcaaata gattcggcaa
      301 cgcaaaatca ttatcatgag tgataattat ccgtgattcc tttttggcgg ttacggcaat
      361 ttctttgtcc gatgaccccc tttttgccaa tttcacatca tgtcccgcgg caagaagcat
      421 ttttgccaga cgtaatttga cattttcatc aacaagaaac ttcatcaaat tttttgattg
      481 tttgcgcgct acctcgttgc gccgcctgcg cggcatagct taacgccgca ttgatatgtt
      541 tcttggtaat tgatggaaag tccgccaaaa tttcttcaac ggtttcgccg gcggcaagca
      601 ttgccaaaac ttgccaaacc atcacgcgag tgccgcgaaa agtcaatttt ccgtggcaaa
      661 tttccgggtc agcgacaata tatttattga ttgaaagcga agccataaat tttaaaaatg
      721 agttaaagaa tgtgttttaa tgatagcaat aaagcccctt ttgtcaattt tgggcaaaaa
      781 taaatccggt ggcgcgattt ggatttccac ccaaatcaca ctgccggata aatacggttt
      841 cacttgctaa cttcggcaag ttttttttcc gcatttacaa tatgcggtgt agccgacttg
      901 ccgctactat gttcatgttg acgatatcgc tttagagctt cttcgtaggc agtatgcgct
      961 tcgccaatac ggccaagagc gcgcaaagca tcgcccttgt tctgttgccc gtgtcccgcg
     1021 gttaaaaagt cgccccttgc cacggatcta tcggtaactc ggtcaccgat tatcactcct
     1081 tgttcgtaat ctttagccca ccgcgcaagg atgcctcgag tattccacgc cgatggttct
     1141 tcaggaaact cctgtaatgc ttcatcgctt gcttcccacg ccccgctttg attgcccgaa
     1201 atccacaaag caagcggtaa gacattgtaa gcggaaagcc gccgcttggg gtcaatagat
     1261 atagagacaa cactggcata tcctgccgcg tggcctgcat ttcttatttt ctcttctggg
     1321 ttgcccttga tggcgatata atatttcgcc catgctctga tccggcaagc ctcggcacac
     1381 ccttccggat ccaatttttg gtcgcaaggc actgcaagat catacgcctc ttgatatttc
     1441 ccttgtctat acaaatcatc cgctcttttt aaagattctt tcataaaatc tcctgctcct
     1501 tcttgatagg aaccagtatt atcaaagcat attttgcagg ttttgtcaaa ttttgggaga
     1561 cggattaaaa tttgaaggca ataataatta ttacgataaa aatactgttg aaggggtcta
     1621 accgcccgcg attacgcggg ggtaagaccc cgaaaaacca aaaagtggtt gaaaaaatgg
     1681 ggcgatggga tatgatggaa gataagtcaa aagtcaaaag gcaaaagcat aagacaaaag
     1741 ttataagata tggcaaatca agcgcaaaca ccgcggtggc aacaacgatt aaaagatttt
     1801 gaaaaatccg ccgcgcggtt aaaagaagcc gcgtccaaaa aagaattcag cgatttggaa
     1861 aaggacggcg tcatccagcg ctttgaattc acttttgagt tggcgtggaa aacattgaaa
     1921 gattaccttg aaaatcaagg tttttcgggt attgccagcc ccaaaaaagc cctgcaaaaa
     1981 tctttttcca tggatttggc aagcgacggc aatgtttgga taaatatgct tgaagatcgc
     2041 aatcgaatgt cgcatcccta cagtcgcgcc gccagcgaaa aaatttttca aaatatcaaa
     2101 aaatattacg tccccgcgat tgatgaactc gccgcgaatt taaagaagca aatttaacca
     2161 tatgtctcac ggattaaccg caaaagaaat caagctgata aaagatgttt ttaaaaaaaa
     2221 ttccgcggtt gagcgagtgt ttatccatgg atcgcgcgcg cgaggcgact tcaaaaaaac
     2281 atcggatatt gattttgccg tcaaattttt tggcaatgac aaaaatctca ttctgcaact
     2341 gcactccgag ctggacgatc tgccggttat ttatgaaatt gacataattg acgaaaaaga
     2401 aattggcggt aaaaatttca aagacgatta cgagaaaagc aaaaaagaat tctacaaaaa
     2461 atcaaaataa caaagggggt tccggacatt tttatttact cgccagaata cctcggggct
     2521 ttgccccggg gaaattaaga aaggtggtag tttttcaact cgcggcgaag atttttttgg
     2581 tcgggaaaag cgattgccag cagggcccaa aatcgcggcg aatggttgaa ttctttcaag
     2641 tggcaaagct cgtgcgccac gatatagccg gccagccgat ccggcaaaaa caccattcga
     2701 taattgaaat tcagattacc cttcgccgaa cagcttcccc atcgcgtccg ctggttgcga
     2761 atcgccacgc ggttgtattt gaagccgtaa accacgctca attcggcgat tttatcaaaa
     2821 acaagccttc gcgccgccgg acgatgctcc aaaaattccc ggcggcgggc aggcggcaaa
     2881 agactttttt ggctttttga ttcggcgatc ttgcccaaaa tccaattcgc tttttggcgc
     2941 aaaaatcttg ccaccaaaaa atcagggaag aattcgggtt tgctggcaaa aacctcgccg
     3001 ccggatttga caacaacgcg cagatttctc gtccgcgcgc ttgttttcaa gcaataagcc
     3061 accttttgcc ccttcaaaat gatttctttg tttttgtttc tcattcctcg ttcctcatcg
     3121 ttcattacta ccgattgtct cccgatccgt gcaaaacccc ccgctctttt cggtcggcca
     3181 gtttttcaat gtttttttgg gcgatatttt ccagatccaa atccaattcc accgccaaat
     3241 tggcgatata ccacaaaaca tcgcccagct ccttttccaa ttcggcgcgg catttatcat
     3301 caacgacatt gtcccggtcg cggacgattt ttttgatttt ttcggccacc tcgccactct
     3361 cgccgcaaag ccccagcgcc ggataaacaa aattttttcc cgccccggca tagatggcgg
     3421 tttttcgcgc caatctttga tattcgctga attccatagg ttttttaatt aaaattatgt
     3481 atgtgcatat ccgttcgcat gtattcgcat atatatccgt ccaagaatac caacactagc
     3541 gatattttgc cgtcagttgg gcggtttcca ggacatggcc ggagatggcg gcgttgaacc
     3601 gttcaatcgg tgcaccgtca ttgagataca ttttcgtgtc cagcgcgtaa agcgtgaaaa
     3661 tgtagcgatg cgttccgctt ggcggacacg ggccgacata accgatcttg ccgttggtgg
     3721 tctttccctg ccgggcgccg ggcggaatcc cgcctatatc aattttttcg gtttttggcg
     3781 gaatgccaaa caatatccaa tggataaaaa ttcctccggg cgcgtcggga tcgcgcaaca
     3841 ccagcgccag cgattcggcc gccgccggca cgccggaaat ttccagtggc gggcttgact
     3901 cggcaccatc gcaagtgtgc gccggcggaa acccgtcatt gttttgaaat atgggactgc
     3961 caagtttcat cgttttttgt gttatttatt tccagcggca tcaaattaac aggcgggcca
     4021 ggcgcaattc ttcaaaagtg gcggtgcctc ccagcaattc cattgccggc gtcagcggat
     4081 aggcgccatt ttcgttttgc gattttttca gcgcggtttt catttgcttt attttttcgg
     4141 gcggcaaatt tttcgccagc ccggaaaaat caaaatcggg aaatttggct ttcagttttt
     4201 cgcaatgcgc caaaatcgtg cccgcggtaa gattccgctt ttcggcaatc tcggccagtg
     4261 aaaatttttg gttaagcaat tcgcgcgtcg ccgccaccgt gtcaactttt tcctttgccg
     4321 gtttcgccgg cgaaaagcgg cgcggcggca aaattttggc ggcaatttgg cgcgcggaaa
     4381 tttccgaccg gcgtttcaaa tccttatcaa actccaaaac ttcggcgttg acttgcaggg
     4441 aaattttgtt gaatcccgcc agcgacaatc ccgccgaaga acgcacgcgc gacagcgcca
     4501 catagcccat ccccggttca aacgactggc gcaaatccac ccgggcggcg tccaagctca
     4561 ttccctggct tttgtggatt gtgatcgccc acgccagccg caaaggatac tggctgattt
     4621 ccgccttgat tttgccttcc tcggcgatgt gccacgccgc cggaccgaca tcaatcgcac
     4681 ctcgggcggt gcgcaccaaa atccgcccct cttccaattc ttgcaccacc cccaaagtgc
     4741 cgttgacgta cccctcttca aaattgttct tcacgaacat cacccgcgct ccggctttca
     4801 gccgcaatac cggcggcgcc agacatcctt tttgcaaacg ctcggccaat gccgccggtc
     4861 cgacaagctc cattttgtag cgaaattcct ttccggaaat tttttgcagt tccagattat
     4921 tttcaacgtc aacatcaaca ttgtgggtgt acagctttgt cggttccacc ggcaaattga
     4981 ttttggcatc cacgcgcgat tgcaaataat ccacgacgct ttcgccggca gagttgcttc
     5041 tgatggcgtt taatgcccgc aaaaactcct gatcgctttg ccgatattgt tcgtccaaat
     5101 agcaaatttt gagatccagc gctttccacg accgcgcgtg atatgaaaat tcggatggcg
     5161 catcttcttc ggcgaaattc aagcttgact gcgccgcttt tttcgcgccg gcaggttcgt
     5221 tttttcgcgc aatcggcggc agttggaaaa aatcgccgca cagcacggtt tgaatgccgc
     5281 caaacggcgc gtcaacctgt ttgaactcgc ggaaaatcaa atccgccaaa tccaattgaa
     5341 tccgcgacaa catggagatc tcatcaatca ccaaaacatc ggcacgcaaa aagcgtcggc
     5401 gcagataacc gcgctttaac aaatctttca cttccgcctt ggaaagcgaa tcacgcactc
     5461 ccatgcccgt ccaggaatgg atcgtaatgc cgtccaggtg cgtggcggca atgcccgtgg
     5521 aagcggtaac cgcgacattg gcgccgctcg ctttcagcca atcaatgtag cggttcaaca
     5581 caaaagtctt gccgctcccc gccggaccgg tgataaacgc gttcttaccc gattttagaa
     5641 tctccattgc ctgttcctgc gtcatttttt taatttttgc tttttatcca ttctataccg
     5701 ccgccggcca tttcacaaat catctcccaa aagtatgctt gctgaatttt gactttcgcc
     5761 gaattagccg ctataatggc attgaaaata ataaaaaaca ggatatgaaa aagaatattg
     5821 tgatcattgc aatcatcttg attgccgcca ttgccggatt ggcaatgatg atgaaccgaa
     5881 aaagtggaga aggaaacaaa aacggcgaag agccggccgg caagctgtcg ccgctcaacg
     5941 ccgcctattt ggtggaaacc gacatgatca cgctggaaaa cggcaaagcc gaaagcatca
     6001 atccctacgg cgcgcagaaa atgaccaccg aagttgatgg ccagccggtt tcgggcgatc
     6061 tgaattccga cggacagaac gattgggcgc tggtgctaaa acagcaaacc gaaaacgacc
     6121 ccgcgattta ttattacgcg gccattgctt tggtggacga agggaagtcg ttgatcgtcg
     6181 gctccaatgc cgtgccgctg ggcgaaggcg tcaagattga agatatcgcc atcgtcaatc
     6241 aagcgatgag gatcaattat ctggaaagga aaattgacgg cgacgctgtg gaggaagaac
     6301 ccaccgtacc ggtatcaaaa agctatattc ttgacggcgc aatgttaaaa gaactcactc
     6361 aaaaacgcgc caacgcgcaa gccgaggccg cctgcaccga caaccgcggc gaatggaata
     6421 tggaaaacca ggaatgttcg ggaatttccc aagaactttg cgaccgtttc ggcggcaaat
     6481 ttgaaaacga actctgcaaa ttttagcccg aaaataatcg caaaaaaaca aaaacgcttt
     6541 ttcggaagcg tttttgtttt gtgccaacga gtctaatcag ctgttgattt cgtttctgcc
     6601 ggcccaggcg atcatcaatg tcgcccaggc aatcagcggg aaaaacacca gcgacaaagc
     6661 gccatagata agatagcggc gctttatctt cgccagaatt ccggccatgc aagcggcaaa
     6721 aaccatcatc ggcgcaaacc accaaacaac ccgaaaaaat aaaagccacg ccaaaagcgc
     6781 ggtcagaaat cccaatgcaa aatcggcgtt tctgagccag cgccgatttt tgtaaatatc
     6841 gcggtcaaat cttttggcgg caaaaccgta cataattcaa attttttaaa ttattaaaaa
     6901 atttcaatgt ttcaatttcc aatttccaaa aagatcattt ctggcattgc gggcaaaaaa
     6961 acgtgccgcg gccggattgg gtttgccgaa ggattttccc gccgcaggca aaacattctt
     7021 gtccgttgcg ctgataaacc aaaaaatgat cctgatagca tcccggactg ccgtccagct
     7081 ggcggtacca ttcgtccttt cccgacgagc cgcggcaatc aatcgccttt tctaaaaccc
     7141 cgacgattga atcaaaaagc tttttgattt ctttttgatc cagcgaatta gccggacgga
     7201 aggggcttac gcgcgcggca aacagcgatt cattggcgta aatgttgcca atgccgccga
     7261 ttttttcctg atccatcaac aaaagcttga tcggcttgcg gctcttcccc aatacgcttg
     7321 agaaattttg caaggtgaaa ttcgggcgaa acggctcaat cgccgcgccg gggtcgtcct
     7381 tgaaatccgc aatcgctttg atccatccga atttccgcga atcattgaaa agcaagcggg
     7441 cgccgctctc aaaataaaag attgcccggg taaatcttgc cggtgtgttg tctcccgcgg
     7501 cgcgccaaaa aaattgcccg tttaatttaa aatgcgccaa aagcgccgcg ccgttggcaa
     7561 gccggatccg caagactttg gcggcgcggc cgacgccggt aatcctggat ccgataacgt
     7621 ctttcggatc gccgataaac attttcggtt ccaaaatttc aattttattt atgacttcgc
     7681 cgacggcttt ttgttccaat tggcggcgaa tggtttccac ttcgggtaat tcgggcacaa
     7741 gattaagtca aaattcaaaa atcaaaagtc aaaagcaaat cttgaaattt aaaattttaa
     7801 attttgaatt catttatcat tgatcattga tcattgatca gtcccaggaa gcgcggccgt
     7861 cgccggtgat gggaatagcc tcccccaaaa aacggggcga atcgttgaaa agaacgcgcc
     7921 aaaacgcttt cacccgcgca ccgttctcgc gccaagtgtt gtaaaatccc ggattatagg
     7981 gtcgccgatc cgccttgatg ccgtgtgacg taataccggc gtgccgcact aaatagagcg
     8041 cgcgcggcaa atgaaattcc tgagtcgcca cgatcaagga accggcttga aaaatctcct
     8101 tggcacgggc ggcgctgtcg taggtgttaa atccggcata gtcaacgaat acatcttcgg
     8161 cgggcacccc gttttgaaga aaaaaatctt tggcaatgtt cacctcgtca taattgtttt
     8221 gactgtgatc gccgctgatc agaatttttt tcgcctttcc ggcatgataa acttccagcg
     8281 cggtggcggc gcgatcggtc aaaatcgcgc tcatccggcc gccgcggtac accgccgcgc
     8341 ccaaaaccaa aaccgcatcc gccgccggca aatcggccgc gttttcatat atcccctgcg
     8401 atccgtgcca aaaaacatag gtgtcggcaa aaatcgcaaa agacgccgcc agcgccgccg
     8461 cgaccaaaac gacgcgcgcc atttttctca aaccttcttt cataatgcca atctaccaaa
     8521 tattgccgat tttgcaaaaa ttttcctcca aatacggcgt ttccccgtta tcatttgaca
     8581 aatttggcgc aaactattga cacgtttttc aaaaaaaggc aaaatgaagc aatattgaaa
     8641 acggcaacct tgacaatttt gtccgatttc atcgccccgc ggcattgcgg gggggttgtt
     8701 tttttaatta ttatgttttc caaaatcctg caaattttgt cttccgccaa aaattgctcc
     8761 aagtgcaacg gcattttgct tgtcatcacc ggtcccagcg gcgtgggaaa ggacaccgcc
     8821 aaggaactgt ttttgaagcg gaatcccggg ttccacaaaa tcgtcaccga caccagccgc
     8881 ttgccgcgcg acggagaaat caagggagtg agccacaatt tttacaccgt tgaagaattc
     8941 aaaaaaagga taaaagaagg aaaatatctg gaatatattg aagtccggcc gcaagaatac
     9001 aaaggcacca gcaaagaatc aatttcggcg attttgagcg gcgaaaacgt gatctggcag
     9061 attgacgaat atgcgatggc gcacatcaaa cggattctca aggaaaatat gcccgacatc
     9121 gccgattgca ttctgcaaaa aaccgtcacc atctatatcg cgcccgagga ttgggaccag
     9181 ttgcgggaac aatatttccg ccgcgatccg aacgcttccg aggaaaaatt tttggggaaa
     9241 ctggcgcgcg accgcgaaat gtggtggcaa tatcatgatc gctacgatca tgtggtcatc
     9301 aacaaacggg gaaagatcaa tgagaccgtt tcaatgattg aaaaactggt cgcgcaaaaa
     9361 gccgaatcct tggtggacgc gcgcttaatc gcgccggcgg caatcatccg ataaaaataa
     9421 aaagacaaaa ccgcgcaaga agcaggtttt tttaatacga aaatgatgga caaaaccgtt
     9481 aaaatcgcca aatatttggc gcaggcgggc gtcgcttccc gccgcaaagc cgaagcgctg
     9541 gtcaaacaag ggctggtttc ggtcaatgga agggtatctg ccgatcccgc ggtcagaatc
     9601 aaccccgaaa ccaacaaaat tctttaccgc ggcaaaccgc tttcgccggc gcaaaaagtt
     9661 tattatcttt tgaacaaacc cgcaggatac acttgcactg cgcaagatcc ccacgccgaa
     9721 aaactcgtcg gcgaactggt gcccgcggaa ccgaaagttt ggtccgtggg gcggctggat
     9781 aagatgacct cgggattgct gatcctgacc aacgacggcg atttggcgct gtccctcacc
     9841 catccgcgct accgggttga aaaggaatat gagatcaccg ccaatcttcc tcttgcggaa
     9901 aatgaaattg ccgccgtcca aaaaggcgtt ttgctggaag acggcttcat ccgccccgac
     9961 cggtttgaaa agatcggcgg caaacgctac cgcatcgtga tccattcggg gaaaaagcgc
    10021 gtggtgcgcc gcatcgttga aaagatcggc aaatccgtgg cgcaactgaa aagaacccgc
    10081 gtgggttctc tcaaactgca agatttgccg gaaggtaaat ggcgccgcct ttcttcggcg
    10141 gaaatcaaga aattggcaca aagaccggcg acgccgacta acgcggcgga ttgaacaaaa
    10201 aatcttccat tattttcccc gtctcccgga aattgttgag caccaaaatg tgattggcgt
    10261 ccggcaaaat ttccaaacgc gcgttgggca ttttttttgc cagccgcgcc gaatccgccg
    10321 ccgggaaaat cgtgtctttc cgtccgtgga cgatcagcac cggcatatcc attccggcga
    10381 taaatttttc tccgtcaaat tttttcgcct cgcgcaaaca acggcaataa accttcaacg
    10441 acgtgtttct gatgtcggca tacattcttc cggcatccca gtcgccggtg ccaaaaaacc
    10501 gacggtaatc aatgtgaacg ccgattcttt cttcaaacgg catcggctcg gtgatgccgg
    10561 caaaaagcaa aaagaagtta acgattttgg cccgccaggt tttgttggcg cgataattcg
    10621 gcgccagtaa aatcaggcgg gaaactttct ttttgtgccg gcgcgcgaaa tccatcgcca
    10681 aaatcgcccc cagggaatgg ccgaccaaaa tgaacttttg cagtttaaac ctttcggcca
    10741 gcgcggcgat atcgccggag atattcttca aaacataata atcgccatcc gcttttttta
    10801 ccgattttcc gtgtccccgc aaatcaaaaa acagcaaatt gaattttttg ccgaattttt
    10861 cctcataggg aatccacgcc gacgaactcc ccgaaagtcc gtgcacaaaa atcattgttt
    10921 cccgtcccgg ccaaaaaccg ttgtgccgcc aataaacccc gttttccaaa tagccttccg
    10981 tcataatttc aaatttttgt tttaccttcc tttcaattaa agattctacc actccccgcc
    11041 ctccggcgca accgatccgc tgtcaaatcc aatccgcctc acccgccggc cataccaagt
    11101 agaacccagc aaacgaaaaa agttatattt aaacatagca tatacatagc acaaacataa
    11161 catcttaaaa aaaacgtgcc gacaaactgt tcgaagcgaa gttccagtcg gcctgccaat
    11221 cgctaatata caagggaaag ccccgggtat tccggcagat gaataaacaa aaactccgtt
    11281 agatcaaacg gaggtatttt ttgcaaatat aaatgttatt ttctgacagg gattatttca
    11341 aaagtgtcgt ggtctttttc aaatttgatt ttatattggt caaaaaatcc ttcctgtcca
    11401 attagcgccc cggcggtaaa attcttgata aaacaagcgg gtatttttac ggcgttaaca
    11461 atatcttgga ctttgatatc cactttatcc aaccgcttgc ccaacgtttc tccaccgata
    11521 ccagtcaatc tatcatctct gctttttgaa agatctattc cggcggcgac tgccagatcg
    11581 taatcaaaca aagaataatc ggcgccggaa tccaaaagcg ccaacacttt ttgagatccg
    11641 ccgtcgccga taagttctat ctccaccatc ggtcttttgg taaattttac tgatctgttc
    11701 ggcgataaaa aacttgtata tttgtatttc ataacaacaa tcaatctgct cgcggcatat
    11761 aaaaagtatc gggagaaaac actttgaata aaacggcgtc cttggttttg agcttgcttg
    11821 tcacatccgc gaccttcttc ccgctcgcca aaactttttt gcggtccaaa gacaaagcaa
    11881 cccattgatt cgcgtaggga gctaatttta tcagattaaa atttatattt tttgtattca
    11941 tataattcag ccaacctaag atgagaatac tacaacccaa cataaaaatc aatttcggat
    12001 ataaaatttt ggcggaaggt ataaaaaggg cgcagaagct gtctggcgcc aataaactac
    12061 gccgaggcaa agccccgagg tattccggca ggcagataaa aattgcatag gtgacaccta
    12121 tgtaatttta tgattcgcgg tggttgaaat acgagcaaaa ttagagtaga attatgttgg
    12181 aagattaagc agagtaaaat tcgccaaaac catggatgcc ttcacgcaaa ttatcaataa
    12241 tcagccgctg gtcgcgggat ttttggcgtg gctgatcgcg caatttatca aaatggtttt
    12301 ggcgttaaaa cacggcatca atcgtgcggc gtttcggcgt ttttgggcca gcggcgggtt
    12361 tcccagttcc cattcctcgg cggtcgccgc gctttccgcc gcctgcgggc ttgtttatgg
    12421 tttcggttcc ccctattttg ccatcgcggc ggttttcgca ttgattgtaa tttatgacgc
    12481 attcactttg cggcgcgaag cgggcaaaca cgccgaagcg ctaaaccaaa ttatggaaga
    12541 agtttatttg aacaaacaaa tcaaaattga acatttaaag gaacttttgg ggcacacccg
    12601 ctttgaggtc tttttcggct tcttgctggg cgcggttgtc gccgttctaa tcgcgggata
    12661 gccaaacaaa aaacgcccga aggcgttttt tgtttggcgg aattcatttt aaccggccgt
    12721 cttctttgag tttggctttc gcgcgctcaa tgccgcgctc acagcgattc ccccatccgt
    12781 caataaatcc ctcgtcgttg aacacgcaca ccacttcgca attattggga cagccgccgc
    12841 attcaacgcc ggccgtggaa aattcaacat ctttgacgcc aaaatcaaaa aatctttcgg
    12901 cttcctggtc gcgcgccaga atggcaatgc ccatcgcccc cattaaatgg ccgatttcgt
    12961 caacgatgat cggctctccg atgatatttt caaacgcgcg cgccacgccg acattcttgc
    13021 tcacacctcc ttggaaaaca atcggcgcgt tgatggtttt ccctttcccc aaattgttta
    13081 aatagttata gacgatggag ttgcacaatc ccgcgatgat gtcttcggtc ggatgcccca
    13141 tctgcgcctt gtgcaccaaa tccgattcgg caaacaccgt gcatctcccg gcaattttgg
    13201 tgggattttt ggatttaagc gcccgggcgc cgaattcctc cacgggaatg ttcaaccgcc
    13261 gcgcctgcga cgacaaaaac gaaccggtgc cggcggcgca cagagtgttc atcgcgtaat
    13321 cggtgatcac gccatcttcc agaatgatga ttttgctgtc ctgcccgccg atctccaaga
    13381 tcgtgcgcac ttcaggatga aacgccagcg tgccgatcgc gtgagcggtg atttcatttt
    13441 tgacgattgt ggcgttgagc accgcgccga tcaattcgcg cgccgatccg gtggtgccca
    13501 ccgcaaacac ctcgccccgg ccggcgattt gccgctggat ttcgtcaagc acccgccgca
    13561 ccgcgccgat gggattgcct tcggtccaaa gataggaacg ggcgaaaatg ttgttgccct
    13621 tgtcaatcgc caccgctttg gtggaaattg atccgatgtc cacgcccaaa tataatttgt
    13681 tatccatttt ttttcttttt ttgtttcata taaagcatat cgcaaaatgc ctccacgcgc
    13741 gtttccagcc cggcttcgga agtttgcgaa tcaaaactga aatacagaat cggcgcgtgc
    13801 cgatcgcggg ccagattgtt gagcgccgcc atcgcgctca cttccggcat acagccgaaa
    13861 ggttttaggt gaataatgcc gtcaaatcct tttttaatca aggaattcgc ccgcgccacg
    13921 gtttcggtgc catgggcgcc gatgtgatgc gtcagataag gatgcgcggc cgcaagatgc
    13981 tttttcacag accgatctcc ggaaaaatat tccgtcaaga ttccggaaac ggtcacgaaa
    14041 cgatgcacct ctatttttcg ttgcgccagc tttttttcca gataaaaatt ggcaaagggt
    14101 tccattaaca tataaagttc gcccaccacc cccacgcgca aacaatcggc gggcttgtca
    14161 atgtcaattt gccggatttg gcgcaggcat tcgtttttga gggaaaaaac cgcccccaga
    14221 gaatatgtcc gggcgatttt ttcaagaaat ttccggtagg ttttttccat ttctcccgga
    14281 tgaatttcaa aaccgatgtt tttgcgcaca caatccgaaa ccgcgtcaat gcaatggatt
    14341 cgcgccaggc aaagcgccag acgatgaatg atcccgggat aggtgttgtg cggcgcgatc
    14401 cgcttgaaat ccttgatgaa accgataacg ctgtttttgt tgttaagttt gaaaaattca
    14461 aattcatagc ccagccgccg cagaatttgt tcctgcactt cggcataata gccgaaccgg
    14521 cacccgccgc cggcctggca aatgacatcg gcgcccgctt ccagcgcttc aatgtagttg
    14581 cccaaattat acttgaaagg aatgcaaacc gtttccggac tgtgtttgaa tcccaactca
    14641 atggttttct tggtgatcgg cggcggcacc agcggccggc cgatcagctc caccaggcat
    14701 ttgaaggccg aataataatc gcccatatgc gggaagctca cttttttttc cggcattttt
    14761 tgaaattaat cacgtcaacg aaactttcca aacgggtttg caatcccacc cggctttcca
    14821 actcgtccaa gctcacgatg atgctgggaa tgcccttcat cttgctttgg caaagattaa
    14881 ccgccagcgc atccgggccg caagggaaag tggttaaaaa aatcatcccg tcaaccgaat
    14941 ctttgtaata tgcggccgcg cccagcaatt ctttgtgata ggtccaataa agcgtttggg
    15001 aaatgttttc cgacattttt ctcgcgcgcg cggcgtccag ccggtcggaa caaagaacgc
    15061 aaactcccga tttttccaga taatcaatga tcggccggcc caaaaaattg tcctccaaag
    15121 tataggaatg agcgacaatc aagatcaaag gcttttcccc gcgccgcgcc gcggcaaatt
    15181 tttccgcctg cgcctctttc ttttcgcgcg cgcttttttc ccacgcgccc ttggctttca
    15241 tgtaggccgc gattgcccgg aacggattgc ccgccaggcg cgcccccaaa cgaatcatac
    15301 tgccggcttc ggtttccagt tttttggcgt taacggaata ttcaatcact ttgattccgg
    15361 gaaaaacatt gcgcacggta tccaccagcg ccataaactt gacgcacatt tcctcgccgg
    15421 aatgcaaaca cgtgatatga ggcacaagaa ccgcgtccgc tttgtctttg agccaatcaa
    15481 tatgcccaac gaaaatttta accgaaagac agctttcgtc aatcgaaagg cgcgcgccgc
    15541 gggcgatgat ttccttgttg gtttcggggc tcaaaacaac attcactccc aattctcgga
    15601 aaaaaacttc ccagagcttg ccgtatttgt gatacaacag cgcccgcgga atccccaccc
    15661 gcaatttgtt cttcttgtcg ccggccgcat tttccacaat ggcaacatac cacctcgccc
    15721 cccttttggc aaccgccacc gcaatttatc cggcatatcc gcctccggat tattcattcc
    15781 acccttcggg cgaaaggttc cagccaaccc ccgcgcaaat catctccccc attttcccga
    15841 ccaacaaaac aaagaattaa atcagagggt ctaaccctct gataaataca cccaaagcga
    15901 aggattttga agaaattttg aaattaaaaa aacttaatca aaatgaaacg attgaaagtg
    15961 ctaatttaaa acaacaaact gaaaccactt ttgacactat caaggccgag cctgacaaag
    16021 ccaaaaaaag acaaatgctt ttggatgttg ccaaagaaaa tcccgatgta gtggaaaaga
    16081 tgatggagat tgccaaagac agggcattag gcttggatta ccgcgaaaga atgattaaac
    16141 agcttggtgt agagagcggc gaacgcgcgg catacatcgc ttccaaaatc aaagagatga
    16201 aaagtaaaga agaaaagaga gaatatgtaa aaaacttgat tgaaaagaaa ttgctgactg
    16261 acgatgtggc taaacagatg ttggaattga tgaagcaaaa gtgattgtaa atggtttgac
    16321 cgaaatcccc gtgttgaatg tttcggtatt cgcttttgcc gaacttgttg ccaacaacaa
    16381 acaaattgcc acaattattt ttttcatatt ttttatattc ctacaaaaaa caaataaaaa
    16441 aacccaaatc caattaagaa ttttaacact gaaa
//
LOCUS       DAOQWE010000004        16212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_4012_length_16212_cov_8.101256, whole genome shotgun sequence.
ACCESSION   DAOQWE010000004 DAOQWE010000000
VERSION     DAOQWE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 16212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16212
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4012_length_16212_cov_8.101256"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..824)
                     /locus_tag="PKK37_00430"
     CDS             complement(153..824)
                     /locus_tag="PKK37_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNR80884.1"
                     /translation="MGINLGQIFTDIFNATLLWAGHHGVRVVLIVLAAAASAALVKAT
                     LSRRKIMPVLDNLPHPAYKYKKVFGAPFFRQTALAEARKQRIATLIKTTNNILRSLIW
                     IVAAIAILPEFGVNITPILASLGLAGLAIGMAAKDILTDFIAGAFIIFEGGYNIGDEV
                     EIAGFSGEVLEISLRRTVLRGGDGVICAVPNREVKLIKKFDGRKKEVEQEDKVKITNK
                     SRPTA"
     gene            complement(896..2572)
                     /locus_tag="PKK37_00435"
     CDS             complement(896..2572)
                     /locus_tag="PKK37_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNR80885.1"
                     /translation="MEKIKTKIKIGENSAKGANIPSGQPLLILKDVAKGYGKEELFCG
                     ARMRVLPRERIAIVGANGIGKTTLLEIIIGKEEPDDGSCEIHKMARIGYLPQETHWES
                     LQNSVIDEIYSADGKMRAMVARKESYEARETAGDLPESEIADYCKFLEEFKAADGYRR
                     QGLIERLLSDFGFAEESRHRKIASLSGGERTKLALAKVILTNPNLIILDEPTNHLDIE
                     TCEWLENFLLHRNVALVCVSHDRYFLDRVCEKTYELTKNGIEKYQCRYSDYVIERQAR
                     RSALEKKYHIQQKYLKEQQEYIDRFRYKATKAAGVQSRIKQLEKMEKIELPREAAKDI
                     KIKLDAGIRRCRAVLKIDGITIGPKDKSLFSLDGKLEVEWGDKVGIIGANGMGKSTLL
                     KTILGKMPPAKGKIALDDRAQIGYYAQAHEEMDHSKILLDEVASKTDESEERVRSVLG
                     ALLFEPFEVEKKKIGDLSGGERARAALAELILRKVNFLLLDEPTNHLDLPSKEVITKV
                     FKEYKGTILLVSHDRYILNEVCDHIWEVKNGELSGYLGNYDDYRYHLSHS"
     gene            2968..4521
                     /locus_tag="PKK37_00440"
     CDS             2968..4521
                     /locus_tag="PKK37_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNR80886.1"
                     /translation="MTIRRHIIKIKKTAAALKKRTSWKWKLGIAITGAILISAATFWL
                     ALPLPQNYETEVPPRPKSYSAKIISLRHDNAKGTILEIKFNADEPALTNLPEYQIEEI
                     FAEAKTRAVFEKISRLENSVDYREIAASPLVEDIKCDIDNGRLIVEVMRQGPYSKARA
                     GGGGNLVTIVFPAAENDYPAISNQKPAANSAVFPMRHTISLEAVLHAPLETASIFINN
                     DPVQFRTEQLAPGEYRFAFETDVQLDKEYRIKAIVADQAGRAAVSQWTLEGQIPSAAA
                     LGKDRFKYLGWWGQINANGVTVRQGASAASEKLGVFSTANRVKVVKEVFGEWVDGKNL
                     WYQIDGGAFAGAYIFSDFVTPMPQPEPPQDIRIPEEIKIGEKWIDVDLSKKIMTLFEY
                     DKPLFATYIAPGREENPTKTGIYRIWYKLAKDEMRGGPPLHSYYYHLKDIPWVMYYNY
                     DYAIHGTYWHDKFGTRQSAGCTNMTQGDAKFIFENTLPELPEGQEEVFAREKPNVGYG
                     TGTVVHNHD"
     gene            4539..5351
                     /locus_tag="PKK37_00445"
     CDS             4539..5351
                     /locus_tag="PKK37_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNR80887.1"
                     /translation="MNKSKKILWLLFAAGMAVFVGYATFEAGWTEIAKTTVRGRNVPP
                     EFIGKKIAFVADIHCGEGFSPARLDGLVKQINLLAPDIVLFGGDYVSRNGKNTEKCFS
                     SLKNINAPLGKFGVLGNHDIEAGKNEVLAAMAGAGIAPLVNENRHINIGGGKITVAGT
                     DETWYGVPDGAKAMEEAADFTIYMTHDPAYFEKYQNDKAKLLLAGHTHGGQITLFGLP
                     FSWLIHRHKYRYEKGVYEEPGRTVIVTNGIGATILPLRFFARPQINLILLEK"
     gene            complement(5421..5909)
                     /locus_tag="PKK37_00450"
     CDS             complement(5421..5909)
                     /locus_tag="PKK37_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR80888.1"
                     /translation="MKNQDKIYVDWIDRVKVLQKAVIFNENGRILALKRADDKNRPHP
                     GCWDLAGGRVESSDIEKWKNKSGKGDDNDILINALRREIKEETGLEVKNICAIHAASA
                     YSESKGMFIMAIGYSCKAADESRLKLSAEHTEFRWAAKEEFMELEIGNDGGFIESFLD
                     KL"
     gene            complement(6049..6279)
                     /locus_tag="PKK37_00455"
     CDS             complement(6049..6279)
                     /locus_tag="PKK37_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR80889.1"
                     /translation="MGIRPIHWKKFEKFLLFVGCSFEREKGDHRIYWREGLSRPVVIS
                     RDEQMPIFVVRNNLRLLDIDTKNYLEILEKIK"
     gene            complement(6284..6568)
                     /locus_tag="PKK37_00460"
     CDS             complement(6284..6568)
                     /locus_tag="PKK37_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80890.1"
                     /translation="MEKQAIKFKLSISIIKEGSRYVAYAPALDLSSSGKTAAHAKKRF
                     IEAAGIFFEEISKKGTFGIVLRNLGWKRVNSIWSPPKIVASETELFCVTA"
     gene            complement(6711..8510)
                     /gene="thrS"
                     /locus_tag="PKK37_00465"
     CDS             complement(6711..8510)
                     /gene="thrS"
                     /locus_tag="PKK37_00465"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNR80891.1"
                     /translation="MNKRYDIEVIRHSLAHIMAAAVKSIYPAAKFGMGPAIENGFYYD
                     FDLGGQTLAPDNLKEIEIKMKKIIGGGVTFERKEIGVDEAKKIFADQPYKLEIISDLE
                     NKGEQKVSVYQSGDFYDLCRGLHVESAKDLPIDGFKLTKIAGAYWKGGEKNKMLTRVY
                     GAAFASKKELDDYLINLEEAEKRDHRKLGKELDLFHIDEDAGLGLVLWHPKGAMLWRV
                     IEDFWYDAHLKGGYDLVRSPHIGNRRLWEKSGHWGFFSGSMYPAMEVGQSLEDLQAGK
                     KPDESEQYLLKPMNCPFHVRIYKNNLHSYRELPMRWAECGTVYRYEKKGELSGLTRVR
                     GFTQDDAHIICRKDQVRDELKKVIDFIAFMYKSFGFEMDKVNVYLSLRDPINKEHYAG
                     DDEGWEFTENILREVAVEKKLNFKEEMGEAAFYGPKLDFKIKDVLGREWQCSTLQFDF
                     NLPERFDMSFVNQKGEQEKPYMLHRALFGSFERFIGLLIEHYAGAFPVWLAPVQAKIL
                     SVGEAHVDFCQKLADEFKQAGIRAEVDSADETLGNKIRKAAGEKIPYVLVIGDKEMQS
                     EMLAVRDRGSKSARAISKPDFIAEVIAKIKNRN"
     gene            complement(8840..9859)
                     /locus_tag="PKK37_00470"
     CDS             complement(8840..9859)
                     /locus_tag="PKK37_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80892.1"
                     /translation="MEKFSSIDGIIGDVGPEGEEEVKECFAYRFKKQNYEGLSEVEKR
                     KTSEESATVQLANQLTNEILRRHDCGIFDIPEQNIHIIKEECWQDEENEATYFWDLQA
                     IGIKERKSAASLLEITMHEMMHMKVRNFVQVPIGLEKSLTNVKAFSNGLNVINRGGGK
                     EYFENLNEAVIEEMVKKEILEYIDSEISDQEIAETKRIKGGLLSGSLKHDLAKNREDI
                     EDILYASCDESGRIKEVDFFCYHKSRKMLDILINKIFQNKENLEANNKNEFNIKARED
                     VFNVFAEATLKGEIRRVIKLVDRVFGLGTMKNLAGFENKTDADGKPFNDIDGQLEFVK
                     KLGEN"
     gene            complement(9869..11656)
                     /locus_tag="PKK37_00475"
     CDS             complement(9869..11656)
                     /locus_tag="PKK37_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNR80893.1"
                     /translation="MELIHNFGKKTIIKTVNDLGGRGLMLGRAGGYLWIGKNPASRYQ
                     GWFCQIGGRLIKIIDDIRPVGAAGFLALENDFWRVKRIWENNRESFFLPTQNCLAYEI
                     EKPAEISFFLDIKESYKNPDAEQHYAVWEEDGLILAVCRQISGLPLPEIFLAVAGDFK
                     ISKITNEWADRDYEWDKKRASPPFCRRAFRPAAVSASRLVFAAGGDKEETAARAGRIL
                     NDFEAEKSKKRTEFLNNYRAKVFWPVLPDKTAVAKAAAKNALRMLVKTEKDSLSLRAG
                     WPWFFQIWQRDEAVSLKGLAEFDRTAATTVFWRQFKELKKNKYHFDTADGAGWLFSRA
                     AEFLKKNFFDGEEKREIADALERAIGWQLRYRTRGGLAVNTDEKTWMDSLERPGACVE
                     IQAQRLAMYDLARRLTDDPEKKEYYSQLKDGLEIIARKTFFDTQNLADRFDVEKNKHD
                     FTCRPNLFLAAYIYPDLLEKSEWRVVFENALAKLWLDWGGLATIDKNDPRFCPQSTGE
                     NAKSYHNGDSWFWINNIAAIAMRRIAGGYFKHYIEKIFVASRHDILWGGALGCASEIS
                     SAQSFDPAGCPNQAWSNATFLELCREFKK"
     gene            complement(11744..13549)
                     /locus_tag="PKK37_00480"
     CDS             complement(11744..13549)
                     /locus_tag="PKK37_00480"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNR80894.1"
                     /translation="MKNIKKTLLSPQADLLLEISWEVCNKIGGINTVLATKAAQMAKR
                     YQDGYFLVGPYLAQNAKHQLEEISPKGWLKTAFDNLKNQGIVCHCGRWLIEGEPQMIL
                     VDFSALWPNVNRYKKELWDDYRVDSMDSGFEFDEPAVWGYAVGRLIEQAALANPAKKT
                     VAHCHEWLAGTALLFAKKFGAGAASVFTTHATTLGRSLAFAGREFYAGIENINPSEEA
                     YRYHVQAKHLLEAAAARECSVFTTVSEITGVECKYFLGREPDVFLPNGLDAGGYLSFE
                     EAAIKHRIQRGRIREFLFYNFFPHYTFDPKNTLFYFLMARYEFHAKGIDSFIVALGDL
                     NRKLKREKSSRTVIAFLWVPAQTKGIHPDLIENRGNYFDIKEFIEENSGNLMESFTYS
                     LFAGKDLKSADVFDGEPHRELERRLRKARRAGAPLICTHELECRNDQIIKALAQAGLE
                     NKKDDCVKVIFYPAYLTGTDRLLNLTMREAIQGCHLGVFPSYYEPWGYTPLEAAAEGV
                     PAVTSDLSGLGRFLAALPRDEQNPGIFIINNFGKSADEAAEQLGGILYDYAVMPRHQR
                     VENKINARKLVNNFDWADLAENYVAAHNLALQSKN"
     gene            complement(13580..>13865)
                     /locus_tag="PKK37_00485"
     CDS             complement(13580..>13865)
                     /locus_tag="PKK37_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80895.1"
                     /translation="VSVVADVEPEKAFWLNDGRMLKNLKELAQALETMDGALWSFHAN
                     GERNDFANWVEDVFGQKQLGSALRKTKSAKTAAKRIKEKMSAPRFWSFLM"
     assembly_gap    13866..13965
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13968..15176)
                     /locus_tag="PKK37_00490"
     CDS             complement(13968..15176)
                     /locus_tag="PKK37_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HNR80896.1"
                     /translation="MPPICLYLHTHQPQRLKKFSVFDIGEHRDYFDHENNRQILKKVA
                     RKSYLPANRMFLDLIRRSRGRFKLSLSITGVLLEQLEAEFPEVLRSFRDLVDTGCCEL
                     VSETYYHSLASVYSAEEFSRQIDLQNKALKKHFDFLPKFFRNTELVYNNSIAAMVEKM
                     GFDGMLAEGWDEILQWRSPNFLYQAKGGALRLLARNYKLSDDIGFRFSNREWKEWPLT
                     AEKYTEWINLLNLDQECQTVNLFMDYETFGEHQWEETGIFKFFEAFFWKTMENPRNRF
                     VLPSKLITEIEPAGELDFWRLVSWADMERDLSAWNGNKMQHAALEKIYALEKDVKEAG
                     DEALLDEWRKLQTSDHFYYMCTKWFSDGDVHKYFNPYESPYESFIAFMNIYNDLKIKV
                     DDILKTKVAA"
     gene            complement(15273..>16212)
                     /locus_tag="PKK37_00495"
     CDS             complement(15273..>16212)
                     /locus_tag="PKK37_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR80897.1"
                     /translation="SVWRRDLMGEVMRYGQIGSAIAAAEKFDVIHCHDWLSLPAGLAA
                     KEVSKKPLVFHVHATEYDRVGTAGLNREICSVERLGFENADAVAAVSAYTKRRIVNDY
                     GAAPGKIVVVPNAVNHSEYAGPADHLLKLKKSGKKIVLFVGRLTFQKGPDYFLRAARA
                     VAAADKSAMFVFSGAGDMERWLIEEAARLGLADRVVFAGFLRGRNLAALYKAADLYVM
                     PSVSEPFGLTSLEALASGAPILISRQSGVGEMVAHCLKCDFWDTGQMAAKMLAALRYP
                     EVREELRQNGLAEVKKFNWVDSARKCLNIYQSVMTA"
BASE COUNT         4027 a         4333 c         3390 g         4362 t
ORIGIN      
        1 ttattattag acctactgct taaatatcca ctactgcaat ttccatattt cggctgcgcc
       61 gaattcgtca gttgtcggca tatttaagcc gatatgcctc ctccctcctc attcggctcg
      121 cgcgaaatct gaaaatttcg tcgcgtaaat atttaagcag taggtctact tttattcgtt
      181 atttttacct tatcttcttg ttcgacctct tttttcctcc cgtcaaattt tttaatcaat
      241 ttaacttcgc gattcggcac cgcgcaaatc acgccgtccc cgccgcgcag aaccgtgcgg
      301 cgcaaagaaa tctccaaaac ctctccggaa aaaccggcaa tctccacttc gtcgccgata
      361 ttgtagccgc cctcaaaaat gatgaaagcg ccggcgataa aatccgtgag aatatctttg
      421 gcggccatcc caatcgccaa acccgccagc cccaagctcg ccagtatcgg cgtgatgttg
      481 acgccgaact ccggcaaaat tgcaatcgcc gcgacgatcc aaatcagcga ccgcaggata
      541 ttgttcgttg ttttaattaa ggtggcgatt cgctgtttgc gcgcctccgc cagcgccgtt
      601 tggcggaaaa acggcgcgcc aaagactttc ttatacttat aggcgggatg aggcaggttg
      661 tcaagcaccg gcattatctt ccggcgcgac agcgtcgcct tgaccaacgc cgccgaggcc
      721 gcggcggcta aaacgatcaa aaccacgcgc accccgtgat gcccggccca taataatgtt
      781 gcattaaaaa tatcggtgaa aatttggccc aaattaatcc ccatatagtc cattataaat
      841 caaaccgccg caaccgccaa caatcgcttt cgcaaccata attttttaca aacgattatg
      901 aatgcgacag atggtagcgg taatcgtcgt aattgcccag ataaccggac aattcgccgt
      961 ttttgacttc ccagatatga tcgcaaacct cgttgagaat gtagcggtcg tggcttacca
     1021 gcagaatggt gcctttgtat tctttgaaga ctttggtgat cacttctttt gacggcagat
     1081 ccagatgatt ggtgggctcg tccagcagta aaaaattgac cttccgcaaa atcagttccg
     1141 ccagcgccgc gcgcgccctc tctcctcccg acaaatcacc gatctttttc ttttccactt
     1201 caaacggctc aaacagcaat gcccccaaaa ccgaccgcac ccgctcctcg ctctcgtcgg
     1261 tttttgacgc cacctcgtca agcaaaattt ttgaatggtc catttcttcg tgcgcctgcg
     1321 cgtaataacc aatctgggcg cgatcatcaa gcgcgatttt accctttgcc ggcggcattt
     1381 ttcccaaaat ggtttttagc agggtggatt ttcccatccc gttggcaccg atgataccca
     1441 ctttgtcgcc ccattcaact tccagttttc catcaagcga aaacaaagat ttgtctttgg
     1501 ggccgatggt gatgccgtca atcttcaaca cggcgcggca acggcgaata cctgcgtcaa
     1561 gtttgatttt gatgtctttt gccgcttccc ggggcaattc aattttctcc attttttcca
     1621 actgcttgat gcggctttgc acgccggcgg ccttggtggc tttgtagcga aaccgatcaa
     1681 tatattcctg ctgttctttg agatattttt gctggatgtg gtattttttt tccagcgcgg
     1741 agcggcgggc ctgtcgttca atgacatagt ccgaatagcg gcattggtac ttttcaatgc
     1801 cgtttttggt caattcatag gttttttcgc aaacccgatc cagaaaatag cggtcgtgcg
     1861 acacgcagac cagcgcgaca ttgcgatgca acaaaaaatt ttccagccat tcgcaggtct
     1921 caatgtccaa gtgattggtg ggctcgtcca aaatgatcaa attgggattg gtcagaataa
     1981 ctttcgccag cgccagcttg gtgcgctcgc cgccggaaag cgacgcaatc ttgcgatgcc
     2041 gcgattcttc ggcgaaaccg aagtcggaaa gcaagcgctc aatcaatcct tggcggcggt
     2101 agccgtcggc ggctttgaat tcttccaaaa acttgcaata atccgcaatt tcgctttccg
     2161 gcaagtcgcc ggcggtttcc cgcgcttcat agctttcttt gcgcgcaacc atcgcgcgca
     2221 tttttccgtc cgccgaatag atttcgtcaa tcacggaatt ttgcaaggat tcccaatggg
     2281 tttcctgcgg caaataaccg atgcgcgcca ttttgtgaat ttcgcatgat ccgtcatcgg
     2341 gttcttcttt gccgataata atttcaagca acgtggtttt gccgatgccg ttggcgccca
     2401 cgatcgcaat tcgttcgcgc ggcaaaacgc gcattctcgc cccgcaaaaa agctcctctt
     2461 tgccgtatcc cttggccaca tcctttaaga tgagcaaggg ctggcccgaa ggaatatttg
     2521 cgcccttggc cgaattttcg ccaattttga tttttgtttt aattttttcc atatgtatcc
     2581 gcgcccgttt tccaacaaaa aaacagggtc gtttaattct tctcgcgcaa cgcgaaaata
     2641 accgccaaaa gcggaacaaa ctccctgtcg ttatgccaac aacttatatt cttgctcgtc
     2701 aaaaaatacc ttcatataat ttccttatcg ttgtttttta ttatgcaaca taacccgcca
     2761 aaaatcaagg acggcaaaat tggcggcaac gtgtttcctt tgctccggca gaagttttga
     2821 cacggcagaa gttttgagtg cggatggttt attgctatga tactttaatg gaaatctccg
     2881 cattgattat taataatcaa tcattgatta attattagtt attgattatt gattattgat
     2941 aaatgattat tggttgaatt ttaatttatg accatccgcc gacacatcat taaaatcaaa
     3001 aaaacggcgg ccgcgctgaa aaaacgcaca agctggaaat ggaaactggg aatcgcgatt
     3061 accggcgcga ttttgatttc cgcggcaaca ttctggctgg cgctcccctt gccgcaaaat
     3121 tacgaaaccg aagtgccgcc gcgcccaaaa tcatattcgg caaaaattat ctccttgcgc
     3181 cacgataacg ccaaaggcac aattttggaa atcaaattca acgccgacga accggcgctc
     3241 acaaatctgc ccgaatatca aattgaagaa attttcgccg aagccaaaac tcgcgccgtt
     3301 tttgagaaaa tatcgcgcct tgaaaacagc gttgattatc gggagatcgc cgccagcccg
     3361 ctggttgaag acatcaagtg tgatattgat aacggccgct tgatcgttga agttatgcgc
     3421 caagggccct attcaaaagc ccgagccggt ggcggcggca atttggtgac catcgttttt
     3481 ccggcggcgg aaaacgatta tccagcgatc tccaaccaaa aaccagcggc aaattccgcg
     3541 gtttttccca tgcggcacac gatcagtctt gaagcggttt tgcacgcgcc gcttgaaacc
     3601 gcctcgattt ttatcaacaa cgatccggtt caattccgca ccgaacagct tgcgcccggc
     3661 gaatatcgct tcgcttttga aacggatgtc caacttgata aggaataccg cattaaagcg
     3721 attgtcgccg accaagccgg acgcgcggcg gtttcccaat ggacccttga aggacaaatc
     3781 cccagcgccg cggcgctggg gaaggatcgt ttcaaatatc tgggatggtg gggacagatc
     3841 aatgccaacg gcgtaacggt gcgccaaggc gcttccgccg cttccgaaaa attgggggta
     3901 ttctcaacgg caaatcgcgt gaaggtggtc aaagaagttt ttggcgaatg ggttgacggc
     3961 aaaaatctgt ggtatcagat cgacggcggc gcgttcgcgg gagcatacat attttcggat
     4021 tttgtcacgc cgatgcccca acccgagccg ccccaagaca tccgcatccc cgaagaaatc
     4081 aaaatcgggg aaaaatggat tgatgtggac ctgtcaaaaa aaattatgac gctctttgaa
     4141 tatgacaaac ctctgtttgc cacctatatc gctccggggc gggaggaaaa tcccaccaaa
     4201 acgggaatct accggatatg gtacaagctg gcaaaggacg aaatgcgcgg cgggccgccc
     4261 ctgcattcct attattacca tctcaaagac attccctggg tgatgtatta caactatgac
     4321 tatgccatcc acggcaccta ttggcacgat aaattcggca cccggcaatc ggcgggctgc
     4381 accaatatga cgcagggtga tgccaaattc atttttgaaa acactctgcc cgaattgccc
     4441 gagggacaag aagaagtttt tgcgcgcgaa aagcccaatg tgggctatgg caccggcacc
     4501 gtggtccata accacgattg accaccgcca agatctgaat gaataaatca aaaaaaattt
     4561 tatggttgct tttcgccgcc ggcatggcgg tctttgtcgg ttatgcgact tttgaagccg
     4621 gctggaccga gattgcaaaa acgaccgtgc gcggcagaaa cgtgccgccg gaatttatcg
     4681 gaaagaaaat cgcctttgtc gccgacattc actgcggcga aggtttttcg ccggcacgcc
     4741 ttgatggttt ggtcaaacaa atcaatttgc tggcgcccga tattgtgttg ttcggcggcg
     4801 actatgtgag ccgcaacggg aaaaacaccg aaaaatgttt ttcttccctc aaaaatatta
     4861 acgccccctt gggaaaattc ggcgttctgg gaaatcacga tattgaagcc ggaaaaaacg
     4921 aagttttggc ggcaatggcc ggcgccggga tcgccccgtt ggtaaacgaa aaccgccata
     4981 tcaatatcgg cggcggcaaa ataaccgttg ccggcacgga tgaaacctgg tatggcgttc
     5041 cggacggcgc caaggcgatg gaggaagcgg cggatttcac aatctatatg acgcacgatc
     5101 cggcatattt tgaaaaatat caaaacgaca aggcaaaatt actgctggcc ggacacaccc
     5161 atggcggcca aatcactttg ttcggcctcc cgttttcatg gctgatccat cgccataaat
     5221 accgctatga aaagggtgtt tatgaagagc ccggacgaac cgttatcgtc accaacggca
     5281 tcggcgccac cattctgccc ctccgatttt tcgcccgccc ccaaatcaac ctcatcctcc
     5341 tggaaaaata aacaccccga agcagaacct cgaggcattc cattctttcg cgaaaaacaa
     5401 agcttaattt ttttgtatgg ttataacttg tccaaaaacg attcaatgaa tccgccgtca
     5461 ttgccgatct ccaattccat aaattcttct ttggccgccc agcggaattc cgtatgttcg
     5521 gcgctcaatt ttaatcggct ctcatccgcc gctttgcacg aatagccaat ggccatgata
     5581 aacatccctt tgctttccga atatgccgaa gcggcatgaa tggcacagat atttttcacc
     5641 tccaatccgg tttcctcttt gatctcgcgg cgcaaggcat tgattaaaat atcgttatcg
     5701 tcgcccttgc cgcttttatt tttccatttt tcaatgtcgc ttgattctac gcggccgccc
     5761 gccaaatccc agcaacccgg atgcggccgg tttttgtcat ctgcgcgttt cagcgccaaa
     5821 atccggccgt tttcgttaaa aattaccgcc ttttgcaaaa ccttcacacg gtctatccaa
     5881 tccacataaa ttttatcttg atttttcata acaacggaca tccattaccg acattctcac
     5941 attcttgaga atgtgagaat gttatttccc aaaacttaaa aaatgaatct cgcaggtttt
     6001 aaactccggc aaactctata atctcaaaaa atgaagccgc gcccggcttc attttatttt
     6061 ttccaaaatt tcaagataat ttttggtatc aatatccaat agccgcaaat tattcctgac
     6121 aacgaaaatt ggcatttgtt cgtcgcgcga gatcaccacc gggcggctca acccctcgcg
     6181 ccaatatatc cgatgatcac ccttttcacg ctcaaaacta cagccgacaa acaacagaaa
     6241 tttttcaaat tttttccaat gtatcggcct tatccccata atttcaagca gttacgcaaa
     6301 acaattccgt ttcgctggca acaattttgg gcgggctcca aatagaattc acccttttcc
     6361 agccaagatt acgcaacact atgccaaaag tccctttctt tgaaatttcc tcaaaaaaga
     6421 tcccagccgc ttcgataaat ctttttttcg cgtgagccgc ggttttgccc gaagacgaca
     6481 aatcaagcgc cggcgcgtag gcaacataac gggaaccctc tttgataatt gaaatcgaca
     6541 atttaaattt tattgcttgt ttttccatat ttttgacgca atcatcgcta ctctttcttt
     6601 atatcccatt ttaaaattta aacaagttac agccatatca cattttgaat attcaattaa
     6661 ccgacattct cacattctca agaatgtgag aatgtccatt attcagtttt ttaattgcgg
     6721 tttttgattt tggcgatgac ttcggcgata aagtcgggtt tggaaatggc gcgggcggat
     6781 tttgagccgc ggtcgcgcac agctaacatt tcagactgca tttctttatc tccaatcacc
     6841 aaaacatagg ggatcttttc gcccgcggct ttgcggatct tgttgcccag ggtttcgtcg
     6901 gcggaatcaa cttccgcgcg gatacccgct tgcttgaatt catcggccag cttttggcaa
     6961 aaatcaacat gcgcttcgcc caccgaaagg attttggcct gcaccggagc aagccaaacc
     7021 ggaaacgcgc ccgcgtaatg ttcaatcaac aatccgataa atctttcaaa cgatccgaac
     7081 aacgcccgat gcagcatata cggtttttcc tgctctcctt tttggttcac aaacgacata
     7141 tcaaatcttt ccggcaaatt aaaatcaaac tgcaaagtgg agcactgcca ttcgcgtccc
     7201 aaaacatctt tgattttgaa atccaatttc ggcccgtaga acgccgcttc gcccatctct
     7261 tccttgaaat ttaatttttt ttcaaccgcc acctcgcgca aaatattttc ggtaaattcc
     7321 catccttcgt catcaccggc atagtgctct ttattgatcg ggtcgcgcaa cgacaaataa
     7381 acattcactt tatccatttc aaaaccgaac gacttataca taaacgcgat aaaatcgatt
     7441 acttttttca gttcatctct aacctggtct ttgcggcaaa tgatatgcgc gtcatcctgc
     7501 gtgaacccgc gcacgcgcgt caatcccgaa agctcgccct ttttctcata gcgataaacc
     7561 gtgccgcatt cggcccagcg catcggcaat tcgcgatagg aatgcaaatt gtttttatat
     7621 atccgaacat ggaatggaca attcatcggc ttgagcaaat actgttccga ttcatcgggc
     7681 tttttgccgg cctgcaaatc ttccagcgat tggccgactt ccattgccgg atacatactg
     7741 cccgaaaaaa atccccagtg cccggatttt tcccataacc gacggttgcc aatgtggggc
     7801 gaacggacca aatcatatcc gcctttcaaa tgcgcgtcat accaaaagtc ttcaattacg
     7861 cgccacaaca tcgcgccctt gggatgccac aataccaatc ccaatccggc atcttcatca
     7921 atatggaata aatccagctc tttgcccaac ttgcgatggt cgcgtttttc cgcttcttcc
     7981 aaatttatca gataatcgtc caactctttt tttgatgcga acgccgcgcc gtagacgcgc
     8041 gtgagcattt tgtttttctc gccgcctttc caataggcgc ccgcgatttt ggttaatttg
     8101 aagccgtcaa tcggcagatc ttttgccgat tccacatgca acccgcggca aaggtcgtaa
     8161 aaatcgccgc tttggtagac cgacactttt tgctcgccct tgttttccaa atcggaaata
     8221 atctccagct tatatggctg gtccgcgaaa atctttttgg cctcatcaac gccgatttct
     8281 tttctctcaa atgtcacgcc gccgccgata atctttttca tcttaatctc aatctctttt
     8341 aaattgtccg gcgccaaagt ttggccgccc aaatcaaaat cataataaaa accgttttca
     8401 atcgccggcc ccatgccaaa cttcgccgcc ggatatatgc tttttacggc cgcggccatt
     8461 atatgcgcca gcgaatggcg aatcacttca atatcgtatc ttttgttcat atttttattt
     8521 atttttaaca ttttatcatt ttgacattta tttgacattt ggattttgga ttttggattt
     8581 tgacattaaa aaaacctcgc cccaaccttg aattttcatt caaaattagg gcgaggtttt
     8641 tgctcccgcg gttccaccta acttgtcccg agtaaaacaa gggatatctc ttttttgcca
     8701 aaaaacttag acctttgacg gttgccggtt ttttcacccg gcaacgcggt tggtggccgc
     8761 gccaatcgcc ttttggcaaa tattcaattg tgatttgata ctacgcccgt tttttgattt
     8821 tgtcaaacaa ctggcaattt taattttcgc ccaatttttt cacgaattcc aactggccgt
     8881 cgatgtcgtt gaacggtttg ccgtccgcat cggttttatt ttcaaaacca gctaaatttt
     8941 tcatcgttcc caatccaaat acgcggtcaa ctaattttat tactctgcga atttcccctt
     9001 ttaatgtcgc ctcggcaaaa acgttaaaaa catcctcgcg agcttttata ttgaattcat
     9061 ttttattatt tgcctcaaga ttttctttgt tttgaaaaat tttatttata agaatatcaa
     9121 gcatctttct tgatttatgg tagcaaaaaa aatccacctc ttttatccgg ccgctctcgt
     9181 cgcaagaggc gtataatata tcttcaatat cttcccgatt ctttgccaaa tcatgtttca
     9241 acgatcccga caataagccg cctttaattc ttttggtttc cgcaatctct tgatcggaaa
     9301 tttccgaatc aatatattcc aatatttctt ttttaaccat ttcttctatc acggcctcgt
     9361 ttaaattttc aaagtattct ttccccccgc cccgattgat aacgttcaac ccgtttgaaa
     9421 acgcttttac gttggttaac gacttttcca aacctatcgg aacctgaaca aagttacgca
     9481 ctttcatatg catcatctca tgcattgtga tttccaacaa agatgctgcc gactttcttt
     9541 cttttattcc gattgcttgc aaatcccaaa aataagtcgc ttcattttct tcgtcttgcc
     9601 agcattcttc ttttattatg tggatgtttt gttccggtat atcaaaaata ccacaatcat
     9661 gtcttctcaa tatctcgttc gttaattgat ttgccagttg aaccgttgcc gattcttccg
     9721 aagtttttct tttttctact tccgacaaac cttcgtagtt ctgtttttta aatcgataag
     9781 caaagcattc cttcacttct tcttcgcctt cggggccaac atcgccgatg ataccgtcga
     9841 ttgatgaaaa tttttccata aaacaatttt attttttaaa ttcgcggcaa agttccagaa
     9901 aagtggcatt gctccaggct tgattgggac agccggcggg atcaaagctt tgcgccgaac
     9961 tgatttcgga cgcgcatccc agcgccccgc cccacaaaat atcgtggcga gaggcgacaa
    10021 aaattttttc aatgtaatgc ttgaaatatc cgccggcgat ccggcgcatc gcaatcgcag
    10081 cgatattgtt tatccaaaac cagctgtcgc cgttgtggta tgatttggca ttctcgccgg
    10141 tgctttgcgg gcagaaccgc ggatcgtttt tgtcaatggt ggccagcccg ccccagtcaa
    10201 gccacaactt tgccagcgca ttttcaaaaa caacgcgcca ctcgcttttt tccagcaagt
    10261 ccggataaat ataggcggcc agaaaaagat tggggcggca agtgaaatca tgtttgtttt
    10321 tttcaacatc aaagcgatcg gccaaatttt gcgtgtcaaa aaaagttttt cgcgcgataa
    10381 tttccaaccc gtcttttaac tgcgaataat attctttttt ttccggatcg tcggtgagcc
    10441 gacgcgccag atcatacatc gccaaccgct gggcctggat ttccacgcat gccccggggc
    10501 gttccagact gtccatccaa gttttttcat cggtatttac cgccaaaccg ccccgcgtgc
    10561 gataccgcaa ctgccagccg atcgctcttt ccaaagcatc ggcaatttcc cgtttttctt
    10621 cgccgtcaaa aaaattcttc ttcaaaaatt cggcggcgcg cgaaaacagc cagccggcgc
    10681 cgtcggcggt gtcaaaatga tatttgtttt ttttcaattc tttgaactgg cgccaaaaaa
    10741 ccgtcgtggc ggcagttcga tcaaattcag ccagcccttt cagcgacacc gcctcatctc
    10801 tctgccaaat ctggaaaaac cacggccagc cggcacgcaa cgacaaactg tctttttcgg
    10861 ttttaaccag catccgcagg gcatttttgg ccgcggcttt ggccaccgca gtcttatcgg
    10921 gcaataccgg ccaaaaaact ttcgcgcgat aattatttaa aaattccgtg cgtttttttg
    10981 acttttccgc ttcaaaatcg tttaaaatcc ggccggcgcg ggcggcggtc tcttccttgt
    11041 cgccgccggc ggcaaaaacc agtcttgagg ctgacaccgc cgccggacgg aaagcccggc
    11101 ggcaaaacgg gggcgatgcg cgttttttgt cccactcata atcgcgatcg gcccattcgt
    11161 ttgttatttt ggaaattttg aagtcgccgg caaccgccaa aaaaatctcc ggcaggggca
    11221 atccggaaat ttggcggcag acggccagaa tcaatccgtc ctcttcccaa accgcataat
    11281 gctgttccgc gtccggattt ttatatgatt ccttgatgtc caaaaaaaat gatatttccg
    11341 ccggtttttc aatttcatag gccagacaat tttgcgtggg aagaaaaaaa gactcccggt
    11401 tgttctccca aattcttttc acccgccaaa aatcgttttc caacgccaaa aaaccggccg
    11461 cgccgacggg gcggatatcg tcaattattt ttatcaagcg gccgccgatt tgacagaacc
    11521 atccctgata gcggctggcc ggatttttgc cgatccacaa ataaccgccg gcccgaccga
    11581 gcatcagacc tctgccgcca aggtcattga cagttttgat tattgttttt ttcccgaagt
    11641 tatggataag ttccacgaat ataaattaaa aaggtaaatt taaaaaggaa aaacgaaaat
    11701 ttcaaattaa aaattttaaa ttgccgtttt tactttttca tttttaattt ttactttgta
    11761 acgccagatt gtgcgccgcc acgtaattct ccgccaaatc cgcccaatca aaattattca
    11821 ccagtttgcg ggcgttaatc ttgttttcca cccgctggtg ccgcggcatc accgcataat
    11881 cgtagagaat gccgcccagc tgttccgccg cttcgtcggc gcttttgccg aaattattga
    11941 tgataaaaat ccccggattt tgctcgtcgc gaggcagagc ggccaagaac cgccccagac
    12001 cgctcaaatc gctggtcacc gcgggcacgc cctcggccgc cgcttccagc ggcgtgtatc
    12061 cccacggttc gtaatacgac ggaaaaactc ccaaatgaca cccttggatt gcctcccgca
    12121 tcgtcagatt aagcaggcga tcggtgccgg tcaagtaagc gggatagaaa ataactttga
    12181 cgcagtcgtc ttttttgttt tccaaaccgg cctgcgccag cgccttgatt atctggtcgt
    12241 tgcggcattc caattcgtgg gtgcaaatca gcggcgcgcc ggcacggcgg gctttacgca
    12301 aacggcgttc cagttcgcgg tgcggttcgc cgtcaaaaac atcggcgctc ttcaagtctt
    12361 ttccggcaaa aagagaatag gtgaaacttt ccatcaaatt gcccgaattt tcctcaatga
    12421 attctttgat gtcaaaataa ttgccgcgat tctcaatcag atcgggatgg atgcccttgg
    12481 tctgcgccgg cacccacaaa aaggcgatca ccgtccggga actcttttcc cgttttaact
    12541 tccggttcaa atctcccaga gcaacgatga aagaatcaat tcctttggca tggaattcat
    12601 accgcgccat cagaaaatag aacaatgtgt tcttcgggtc aaaagtgtag tgcggaaaaa
    12661 aattatagaa caaaaattcg cgaatccggc cgcgttggat gcggtgcttg atcgccgcct
    12721 cctcaaaact caaatacccg cccgcatcca atccgttggg cagaaaaaca tccggttccc
    12781 gccccaaaaa atacttacac tccacgccgg tgatttcgct caccgtggtg aacaccgaac
    12841 actcgcgcgc ggcggccgct tccaaaagat gcttggcttg gacgtgataa cgataagctt
    12901 cctccgacgg attgatgttt tcaatgccgg cataaaattc ccggccggca aacgccagac
    12961 tgcgccccag ggttgtcgcg tgcgtggtaa acaccgaagc ggcgccggcg ccgaattttt
    13021 tggcgaacaa cagcgccgtg cccgccagcc attcatggca atgggccacc gtttttttgg
    13081 cgggattggc cagcgccgcc tgttcaatca agcggccaac ggcatatccc caaaccgccg
    13141 gttcgtcaaa ctcaaaaccg gaatccatgg aatcaacgcg gtaatcgtcc cacaattctt
    13201 ttttatagcg attcacattc ggccaaagcg cggaaaaatc aaccaaaatc atctgcggct
    13261 ccccttcaat cagccatctt ccgcaatggc agacgatgcc ttgatttttc aggttgtcaa
    13321 aagcggtttt gagccatcct ttcggcgata tttcctccag ctggtgtttc gcgttttgcg
    13381 ccagatacgg gccgaccaaa aaatagccgt cttgatagcg cttggccatt tgcgcagctt
    13441 tggtggccaa aaccgtgttg ataccgccga ttttattgca aacttcccat gaaatttcca
    13501 acaacaaatc cgcttgcgga gacagcaatg ttttttttat atttttcatc ggcgttaagg
    13561 cgggggcgtt ttaatcgggc tacatcaaga aagaccaaaa ccggggggcg ctcatttttt
    13621 ccttgatcct cttggcggcg gttttggcac tcttggtttt gcgcaacgcc gaaccaagct
    13681 gtttctgccc gaagacatct tccacccagt tggcgaaatc atttctttcg ccattggcgt
    13741 ggaaactcca aagcgcgccg tccattgttt ccaacgcctg cgccagctct ttgagatttt
    13801 tcaacattcg tccgtcattt aaccaaaacg ccttttccgg ttcaacgtcg gcaaccacgg
    13861 acactnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcta tgcggcgacc
    13981 tttgttttta aaatatcgtc aactttaatc ttcaagtcgt tgtaaatgtt cataaacgcg
    14041 ataaacgatt cataggggct ttcataggga ttgaaatatt tgtggacgtc gccgtccgaa
    14101 aaccatttgg tgcacatata atagaaatgg tcgctggtct gcagtttgcg ccactcgtcc
    14161 aacaatgcct catcgccggc ttctttcacg tctttttcca gcgcgtatat tttttccagc
    14221 gccgcgtgct gcattttgtt gccgttccag gcgctcaaat cgcgttccat atccgcccag
    14281 gaaaccagcc gccaaaaatc cagttccccc gccggctcaa tctcggtaat caatttcgac
    14341 ggcagaacaa atcgattgcg cggattttcc attgttttcc aaaagaacgc ctcaaaaaac
    14401 ttaaatatcc ccgtttcttc ccactgatgc tcgccaaacg tttcataatc cataaagaga
    14461 ttcaccgtct ggcattcttg atcaagattc aaaagattga tccactcggt gtatttttcc
    14521 gcggtgagcg gccactcttt ccactcgcga ttggaaaaac gaaagccgat atcgtcgctc
    14581 aatttgtaat tccgcgccag caagcgcagg gcgccaccct tggcttgata aagaaaattg
    14641 ggcgaacgcc attgcaaaat ttcatcccag ccttcggcca gcattccgtc aaagcccatt
    14701 ttttccacca ttgcggcgat actgttatta tacaccaatt ccgtgtttcg gaaaaatttc
    14761 ggcaagaaat caaaatgttt tttcagcgcc ttgttttgca aatcaatctg gcgggaaaat
    14821 tcttcggccg aatagaccga tgccagcgaa tgataatagg tttccgaaac cagttcgcag
    14881 catccggtat caaccaagtc gcgaaagctt cgcaaaactt cgggaaattc ggcctccaac
    14941 tgctccagca aaactccggt gatgctcaag ctcagcttga atcttccccg cgaccggcgg
    15001 atcaaatcca aaaacatgcg gttggccggc agatagcttt tgcgggcgac ttttttcaaa
    15061 atttgccggt tgttttcgtg atcaaaataa tcgcggtgtt cgccgatgtc aaaaaccgag
    15121 aattttttca atctctgcgg ctggtgggtg tgaagatata ggcaaatggg aggcatgttt
    15181 caaattaaaa atttaaacat caaaattaaa aattaaggta aaatttcttg cgaaattttg
    15241 attttaaaat ttttcatttt acatttttga ttttaagcgg tcataacgct ttggtagatg
    15301 tttaaacatt ttcgggccga atcaacccaa ttgaattttt tgacttcggc cagtccgttt
    15361 tgccgcaatt cttccctcac ttccggatag cgcaacgccg ccagcatctt ggccgccatt
    15421 tggccggtgt cccaaaaatc gcatttcaaa caatgcgcca ccatttcgcc gactccggat
    15481 tgccgcgaga tcaaaatcgg cgcgccgctg gccagcgctt ccagggaagt gagcccgaag
    15541 ggttcggaaa ccgacggcat cacatacaaa tccgccgctt tataaagcgc ggccaaattc
    15601 cgcccgcgca gaaaaccggc aaaaaccacc cggtcggcca gccccagccg cgcggcctct
    15661 tcaatcagcc atcgctccat atctcccgcg ccggagaaca caaacatcgc gctcttgtcc
    15721 gccgccgcca ccgcgcgcgc cgcgcgcaaa aaataatccg gccctttttg aaaggtgagc
    15781 cgccccacaa acagcacgat ttttttgccg ctctttttca attttaacaa atgatcggcc
    15841 gggccggcat attcgctgtg gttaaccgca ttgggcacca cgacgatctt tccgggtgcg
    15901 gcgccgtagt cgttgacgat ccggcgtttg gtataggcgc tcaccgcggc cactgcatcg
    15961 gcgttttcaa atcctagtct ttccaccgaa caaatttcgc gattcaatcc ggcggtcccc
    16021 acccggtcat actcggtggc atgaacgtgg aacaccagcg gtttttttga aacttcttta
    16081 gccgccaatc cggcgggcaa actcagccag tcatggcaat ggatcacgtc aaatttttcc
    16141 gcggcggcga tggcgctccc gatttggccg taacgcatca cctcgcccat cagatcgcgc
    16201 cgccagactg aa
//
LOCUS       DAOQWE010000005        15680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_4197_length_15680_cov_7.490880, whole genome shotgun sequence.
ACCESSION   DAOQWE010000005 DAOQWE010000000
VERSION     DAOQWE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 15680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15680
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4197_length_15680_cov_7.490880"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKK37_00500"
     CDS             <1..302
                     /locus_tag="PKK37_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNR80898.1"
                     /translation="NNLNPVDLRQDAGALWENFMISERVKHNDNISRAANMYFWRTLQ
                     QQEIDLIEDRGGELAGYEFKWKSRPGKVPVAFSRAYPDAKVEMITKENYKTFVGL"
     gene            530..1672
                     /locus_tag="PKK37_00505"
     CDS             530..1672
                     /locus_tag="PKK37_00505"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR80899.1"
                     /translation="MKVDFINSSYRRYYQAHKAQMMKALDRCFSKGDFILREDVARFE
                     KNLADFCGVKYAAGVNSGTDALKIAYKALGCGQGDEVITVGHTFIASIEEIVHLGAKP
                     ILIDVGEDGLMNVDQIEAAITEKTVGIVPVHLSGKVCDMARIMAIAKKHNLWVVEDAC
                     QALGAHFDGKPAGSFGHAACFSFISPKTLGCAGDAGGVITNDRKIYEKILLLRNHYNI
                     TQNALLGHQPKAPKIMGWGYNSRLDNIQAALLNIKIKYYPQMLKRRRQIAEKYNKGLT
                     GLPVVLPTQQPEQIYQEYIIKVGNIWKFKKYMQDRNVELLVRDTVPNHKMPGLGLDRF
                     NLPVTERIAKELVRLPIYPELTDAEVDYVIKCVREYFLPAVKKSAK"
     gene            1697..3565
                     /locus_tag="PKK37_00510"
     CDS             1697..3565
                     /locus_tag="PKK37_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNR80900.1"
                     /translation="MEIVRILAKNKKVAFFVADAVLVALSVWLAFMIRFEAAVPGQYF
                     EIIARLVLIGWIFYLPALAANRLYSFSWSYVSASELIALARAVGLGFLLQTAVLYLFR
                     DFAFFSGFPRSTIFVAAFFVFVLCGGIRFAKRIYFYFFRRNNGEGVARVLIAGAGDAG
                     EQLLRSIQSSGADRYFAAGFVDNNDSRQGSIIHGAKVLGKIAQIPEVVSRHNIGELII
                     ALPPGSKSVREAVRLAKTAGLKKIRIVPPLADIIDGKFSLANVRRVEVEDLLERDKVS
                     LDMDAIGKFITGKKVLITGAAGSIGSELCRQTAKFKPAELTVLDQDETGIFNIGNELK
                     KHFADLPVKEFVADIQNASRMKEIFLEIGPQIVFHAAAYKHVPLMEQNAGEAVRNNVF
                     GTKNLADAAISAGAQNFIFISTDKAINPTSVMGATKRIGEMICQAANGAGKTKFVSVR
                     FGNVLGSRGSVIPIFREQIRRGGPVEVTHPEMKRYFMLTSEACLLVMQAGALGKGGEV
                     FVLDMGKPVKIVDLAKEMIKLSGFQPDIDMPVVYIGIRPGEKLFEELMAGDEAVSATQ
                     SEKIFIAKLAAADERSLAEKLESLKNAARMPEKQSQVVLKLRSLVPEFCNNEKQAR"
     assembly_gap    3865..3964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3965..4378
                     /locus_tag="PKK37_00515"
     CDS             <3965..4378
                     /locus_tag="PKK37_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80901.1"
                     /translation="TAECADPSAAKNLLSQVAQNIIAEHDKTIAARRQTIEDQVKNYQ
                     ANAEEIKNSFQYSADRSCSTERYLAASTLKNRIIELEFALSRLDGTAVVLAPTVSETP
                     VKPNPGLNVSLGLVLGFFAGIFAALAKNWWSNAKN"
     gene            4378..6363
                     /locus_tag="PKK37_00520"
     CDS             4378..6363
                     /locus_tag="PKK37_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR80902.1"
                     /translation="MIKNEGGIWVLLVKIGIFAALLTPVIVDFDLYFPFVASKGLWFM
                     ACAQLAFFSWLVLAWHWRHYRPNFKNPVVAAVLAYLGVLFLSAILGADFSASFWSTFE
                     RMGGVLMFCHLAGFMISAALVLEARDWRNVFFASAAVALAVGLRALFDQSAAARGGGF
                     LGNDSFLGTYLLFNVFIALYLFWSGKNAGAGRGAAVAGIVFAALAFCLLVQGTQLWAG
                     IISGGFVLPEAGIIKDIFNSGARAAKISFLGGLVLLEGLRLVLLAKKTLLKKIGIIAV
                     CLGIAAAAAVWGSFQNGGAGKQFFTDNFGESTVASRLAVWEIAWRGFLERPVFGWGPE
                     NFGLVFARHYNPCLGSSACGGATWYDRAHNIVFDTLAETGIAGLIAYLAVFAAALFVL
                     WQNRRRQNGIPAAAVFPALFAAYFVQNLTVFDMPASHLMLFLCLGFIAANFDQGGQKP
                     KTLSRPPDGRILAGVAVAATICFAAFVLSPWSASAGVIGASREPFFSAAKLDLHQRAL
                     EASPLGIRQVRLFFARQWLNASEDKEFIEKAREGEIPMGEINRYFNFFTGELKKSGAD
                     CEFDFASRLELGRLYRQWSVFDRAKIPAAVEALESARSLSPRNQQPFWELAQMKLYEN
                     NVAGAEESARQAHDLYPENVQSRAILEEIQKIRENRQ"
     gene            6497..7111
                     /locus_tag="PKK37_00525"
     CDS             6497..7111
                     /locus_tag="PKK37_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80903.1"
                     /translation="MRFIEFKEKLKGFKIFSLTDIRKNDPDFDLRRLNEWQKKKYIKK
                     IRKGWYIFSDLEINESGLFLIANKIYAPSYVSLEMGLSQYNLIPEMVYEITCVTSQKT
                     ASFKTGIGNFSYRHIKPELMFGYELRQQNDYLYAIAGIEKALLDYLYLKPKIKENEDF
                     QGMRFNYDEFKERADMKKWEKYLTAFGNKALTVRAKKFIKYFNA"
     gene            7104..7883
                     /locus_tag="PKK37_00530"
     CDS             7104..7883
                     /locus_tag="PKK37_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010424547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR80904.1"
                     /translation="MLNLSQITEYYSAPERKFKRNILREYLQYKILEIIFNSKAGQKM
                     SFLGGTALRIVYGSPRFSEDLDFDNFSLTEKDFAELADKIRVDLAKQGYRVEIKNIFK
                     GAYRCYVKLPEVLFDNELSNLRQEKIVIQVDTAPHLFGYDRELKNINKFDVFTQIYAT
                     PVDIILSQKIYAALNRKRTKGRDFYDIVFLLSRTMPNYEYLNAKLGVKNGSDLKKMLL
                     SKTADFDFETMAEDVKPFLTDDSGVKRVKLFLPYIKSLEMK"
     gene            7880..8761
                     /gene="murB"
                     /locus_tag="PKK37_00535"
     CDS             7880..8761
                     /gene="murB"
                     /locus_tag="PKK37_00535"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNR80905.1"
                     /translation="MIMIKKNEPLANHCTYRIGGPARVLFLAANEREIIAGIETAEEN
                     RFKMMILGGGSNILFSDRGFDGLVLKLANNFIKIAEEKEDRAVVLAGAGAPLAALVKF
                     ALDNSLAGMEWAAGIPGTAGGAIRGNAGAFGREIGAAVLRVRALEISGKSIVPAIVGK
                     EDCAFGYRESVFKRNKNLVITAAKFVLEKGNANEIADKIKECAEKKRISQPLDFPSAG
                     SVFKNPEGFFAAKIIEDCGLKGRSAGGAQISEKHANFIVNRGNAKADDVLALIAQIKA
                     AAKEKFAVDLKEEIEIV"
     gene            9370..9717
                     /gene="raiA"
                     /locus_tag="PKK37_00540"
     CDS             9370..9717
                     /gene="raiA"
                     /locus_tag="PKK37_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNR80906.1"
                     /translation="MNIIVKTKNFELTPSLRKFAEEKISSLKKYFDFFQTEDAPGLKS
                     KIDAAIELGKTTAGQRKGNIFRAEVLITFHRNKLRAGVSADDIEKAIVGARDDLQRQI
                     TDFKEKLLDKTRK"
     gene            10170..12614
                     /gene="secA"
                     /locus_tag="PKK37_00545"
     CDS             10170..12614
                     /gene="secA"
                     /locus_tag="PKK37_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNR80907.1"
                     /translation="MSFLTRIFGDENKNYFKKLKPLAEKINSLEKDLRDLDDGRLKAK
                     TAGFKERLEKGETPDDLLPEAFAATREAARRTLGQRHFDVQIIGGIVLHQGRIAEMKT
                     GEGKTLTATAPVYLNAISGKGVHLVTVNDYLARRDCVWMGQVYHALGLSTACINHDQS
                     FLYDPQYKKADETEDAVRDRLGSFRVSQDFLRPCSRREAYAADITYGTNNEFGFDYLR
                     DNMAYRREDKVQRGFNFAIVDEVDSILIDEARTPLIISAPDQESSKWYRDFARIIPRL
                     SAQTDYNIDEKDRAATLTEDGIAKVEKIMGMDNIYEEKGVKYLHHLEQALRAQALYRK
                     DRDYVVKDGQIVIIDEFTGRLMPGRRWSGGLHQAIEAKEEVAVQAESLTLASITFQNL
                     FRMYKKLAGMTGTAMTSAEEFDKVYNLDCVPIPTNKPQVRVDLPDRIYKTEAAKFKAV
                     VAEIKERHRAGQPVLVGTRSVEKNEYLGMLLAREGVKHEILNAKNHEREGEIIAQAGR
                     FGAVTIATNMAGRGVDIILGGNPPDAGEAEKVRGAGGLFIIGTERHEARRIDNQLRGR
                     GGRQGDPGTTQFFISTEDDLARIFAGDTMKKLMEMLKVPDDMPIESGMVSKRIEEAQT
                     KIEGSHFDIRKHLLDYDGVLNKQREAIYKKRDEILAQDDESFRAFVLDLAQKGSAREQ
                     YEKREEENGREAMLQAGRFIALRTIDFFWQEHLELMDNVRDATRLRAYGQRDPLVEYK
                     SEANRQFRVLLETVDANILQNLLNVTVNKNAPAASQSVPSAGAPRQSAAKYAGANRND
                     PCPCGSGKKYKKCCGK"
     gene            12651..12914
                     /locus_tag="PKK37_00550"
     CDS             12651..12914
                     /locus_tag="PKK37_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017749026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80908.1"
                     /translation="MSTITKNNNHKRIDITLPPETLVLLEKISRKGDRSRLIDEAIRF
                     YVKEKNRAKLRKDLKEGAIKNAKLNLSIAEEWSSLENEAWPKL"
     gene            12899..13261
                     /locus_tag="PKK37_00555"
     CDS             12899..13261
                     /locus_tag="PKK37_00555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNR80909.1"
                     /translation="MAETLTPKRGEIYLVGFDPAIGSEIKKTRPALVIQNDIANRYSP
                     VVIVSAISSKFGPKIYPTEVFLQPPMGNLKIPCVVKLDQIKTVDKKRLIKKIGCLDAE
                     MMILVNRAAAISLELIDI"
     gene            13302..13730
                     /locus_tag="PKK37_00560"
     CDS             13302..13730
                     /locus_tag="PKK37_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007139745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR80910.1"
                     /translation="MEKLLHEPKAGIGVMVLKDGKVLLGKRKGAHGAGEFAFPGGHLE
                     YMESFADCARRETAEECGIKIKNIRFRFLANLTEYAPKHYVHIGLAADWESGAPKVLE
                     PEKSESWHWYELDNLPCPLFKTCVLAIESFQTGVNYFDIS"
     gene            13885..15339
                     /gene="secD"
                     /locus_tag="PKK37_00565"
     CDS             13885..15339
                     /gene="secD"
                     /locus_tag="PKK37_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNR80911.1"
                     /translation="MKKNHGWALLFILALAAVSAAFCFPGAVNRGIAAVNAKLPFAIP
                     PMAEKPFNLGLDVKGGVRLEYQADLNQIAEDDRAEVMEGVKDLIERRINSYGVAEPQI
                     QIIGENRLAVELPGIESVQQAIEWIGATPWLEFSEPRPEEETQKILDKIKEMEGKTSE
                     EIAQISDLETLFDEQGNLKNPYFMPTELTGKYLKSARFDFDPNTNRPVVELEFDEEGA
                     KIFEQITERNVGKALAIYLDGFAIIDTSGDGQIDNSDLYAPNVNEKISGGKAVITGIA
                     GTQEAKTLVKRFNEGALPVPLGEPISQQKIGPTLGAVSLDKTVAAGLIGFLAIAVFMI
                     FYYRLPGLLASLALALYGVILLAAFKLMGVTMSLAGIGGFILSLGMAIDANILIFSRM
                     KEELAGGKSLTAAIEEGFVRAWPAIRDGNFTTIAVALILFFLGTSFVKGFAATLIWGI
                     LLSMYSAIVVTRTFLRIFSGTRLGKAVWLWKPLF"
     gene            15457..>15680
                     /locus_tag="PKK37_00570"
     CDS             15457..>15680
                     /locus_tag="PKK37_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNR80912.1"
                     /translation="MISFIKHKNIFFVLTLAAIAAAVAAISVWGLRPGIEFTGGSLTI
                     VEYHDARPETGAIREKVQSLGLADASVYAV"
BASE COUNT         4122 a         3223 c         4277 g         3958 t
ORIGIN      
        1 tcaacaattt aaatcccgtt gatttgcgcc aagacgcggg cgcgctttgg gaaaatttta
       61 tgatcagcga acgcgtaaaa cacaatgaca acatcagccg tgccgccaat atgtattttt
      121 ggcgcacttt gcagcagcaa gagatcgatt tgattgaaga tcgcggcggc gaattggccg
      181 gttatgaatt caaatggaaa tcgcggcccg gcaaagttcc ggtggcattt tcgcgggctt
      241 atccggatgc caaagtggaa atgatcacca aagaaaacta taaaaccttt gtcggattgt
      301 aacttttatc caaaaccggc gacgagaaag caaggatggc gtccaatctc atatctgcga
      361 cggtatacca aggtcaattt ggcaaaaata gtgcggcgtt gattatttat tcgccgtctt
      421 tttttatccg catattattt tttattttgt caagtggatt tttgggaaaa ataagtatat
      481 aatacaggtg aagtgttttg gaataataat caaaaataat taaaaaccaa tgaaggtgga
      541 ttttatcaat tcaagctatc ggcggtatta tcaggcgcat aaggcgcaaa tgatgaaagc
      601 gctggatagg tgtttttcaa aaggggattt tattttgcgc gaggatgtgg cgcggtttga
      661 aaaaaatttg gcggattttt gcggagtcaa atatgcggcg ggcgtcaatt ccggcaccga
      721 tgcgcttaaa atcgcttaca aggcgctggg atgcggtcag ggagacgagg tgatcaccgt
      781 ggggcatacg tttatcgctt ccattgaaga gattgttcat ttgggcgcca agccgatatt
      841 gattgatgtg ggcgaggacg gtttaatgaa cgttgaccaa attgaggcgg ccattaccga
      901 aaaaaccgtg ggcatcgtgc cggtgcattt atcgggcaaa gtgtgcgata tggcacgaat
      961 aatggcgatt gccaaaaagc ataacctttg ggtagtggaa gacgcttgcc aggcgctggg
     1021 agcgcatttt gacggcaagc ctgcgggcag tttcggccac gcggcttgtt tcagttttat
     1081 ttctccgaaa acgttgggtt gcgcgggaga cgcgggaggc gttatcacca acgacagaaa
     1141 aatttacgaa aaaattttgc ttttgcgcaa tcattataat attacgcaaa acgcgttgct
     1201 ggggcaccag cccaaggcgc ccaaaatcat ggggtggggt tataattcgc gtttggataa
     1261 tattcaggcc gcgttgctaa acattaaaat aaaatattat cctcaaatgc ttaagcgccg
     1321 ccgccaaatt gccgaaaaat ataacaaagg actgacggga ctgcccgtgg ttctgccgac
     1381 gcaacagccg gagcagattt atcaggaata tatcattaaa gtcggcaata tttggaaatt
     1441 caagaaatat atgcaagatc ggaacgtgga gctcttggtt cgcgatacgg tacccaatca
     1501 caaaatgccc ggactgggac tggatcgctt taatttgccg gtaaccgaaa gaattgccaa
     1561 agaattggtg cgtttgccga tttacccgga gcttaccgac gccgaggttg attatgtgat
     1621 caagtgcgtg cgggaatatt ttttaccggc agtgaagaaa agcgccaaat aataccacaa
     1681 ataacggagt aattttatgg agattgttcg gattttggcg aagaacaaga aggtggcgtt
     1741 ttttgttgcc gacgcggtgt tggtcgcgct ttcggtttgg ctggcgttta tgatccgctt
     1801 tgaggccgcc gttcccggcc aatactttga aatcatcgcg cgactggttc tgattggctg
     1861 gatattctat ctgcccgcgc tggcggcaaa tcggctttat tctttttcgt ggtcctatgt
     1921 gagcgccagc gaattgatcg cgctggcgag ggcggttgga ttgggatttt tgctacaaac
     1981 cgcggttttg tatcttttcc gggattttgc ctttttctcg gggtttcccc gctccacgat
     2041 ctttgtcgcg gcgttttttg tttttgtcct ttgcggcggg attcgctttg ccaaaagaat
     2101 ttatttttat tttttccgcc gcaataacgg cgagggagtc gcgcgtgttt taatcgccgg
     2161 cgcgggcgat gcgggggaac agcttttgcg cagtatccaa agttccggcg cggaccgtta
     2221 ttttgccgcg gggtttgttg acaacaacga ttcccgtcag ggaagcatca ttcacggggc
     2281 gaaagttttg gggaagatcg cgcaaattcc cgaagttgtt tcgcggcata acattggcga
     2341 gttgatcatc gccttgccgc cggggtcaaa atccgtgagg gaggcggtgc gtttggccaa
     2401 aaccgccggc ctcaaaaaaa tcagaatcgt gccgccgctg gctgacatta ttgacggcaa
     2461 attttcgttg gcaaacgtgc gccgggtgga ggtggaggat ttgttggaac gggacaaggt
     2521 gtcgctggac atggacgcaa tcgggaaatt tatcaccggc aaaaaagttt tgatcaccgg
     2581 cgcggcggga tcaatcggat cggaattgtg ccgccagacg gccaaattca aaccggcgga
     2641 attaacggtt ttggatcagg acgaaaccgg aatttttaac atcggcaatg agttaaaaaa
     2701 gcattttgcg gacttgccgg tcaaggaatt cgtcgccgac attcaaaatg cgtcccggat
     2761 gaaagaaatt tttttggaaa tcggcccgca gatcgttttc cacgcggcgg cctataagca
     2821 tgtgccgctg atggagcaaa acgccggcga agcggtgcgc aacaatgttt tcggcaccaa
     2881 aaatcttgcc gatgcggcga tttctgccgg cgcgcaaaac tttattttca tttccaccga
     2941 caaagcgatc aatccgactt cggtgatggg cgccaccaaa cggatcggcg aaatgatctg
     3001 ccaggcggcc aacggcgccg gaaaaaccaa attcgtgtcg gtgaggttcg gcaatgtttt
     3061 gggaagccgc ggcagtgtga ttccgatttt tcgcgaacag atccgccggg gagggccggt
     3121 ggaagtgact catccggaaa tgaaacgtta tttcatgctc acttcggaag cgtgtttgct
     3181 ggtaatgcag gcgggggcgc tggggaaggg gggagaagtg ttcgttttgg atatgggcaa
     3241 acccgtgaaa atcgttgatt tggccaaaga gatgatcaaa ctgtcggggt tccagcccga
     3301 tattgatatg cccgttgttt atatcggaat tcgtcccggg gaaaaacttt ttgaagaatt
     3361 gatggccggc gacgaggcgg tgagcgccac tcaaagcgag aaaattttta ttgccaagct
     3421 ggcggcggcg gatgagcggt ctttggcgga aaaattggaa agcctgaaaa acgccgcgcg
     3481 aatgccggaa aaacaatccc aagtcgtttt aaaactgaga tccttggtgc cggaattttg
     3541 caataacgaa aaacaagcaa gatagttgaa ttgatgattt gaatttgatt agccaatcgg
     3601 ggaagtctga tgactggcta aattaatata taattcagtc agtttgatgc ctaaattata
     3661 taataattaa gtgaaggtgt ttcgtaatcc aaattaatta aaaactatgg aaaaagaaaa
     3721 tcaatattca gagagcgttt ccgaatatga agaagttgat ctgcgcgacg tttttaatgt
     3781 tttaatccgg aaaaagaaga ttattgtttt ttgcgcggcg gcgggattgg cggcggcgct
     3841 ggttgccaat gccgttttgc cgcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnaccgcg gaatgcgccg atccttcggc ggcaaaaaat ttgctttcac aagtagcgca
     4021 aaatataatc gccgagcacg ataaaacaat tgccgcgcgc cggcaaacaa ttgaagacca
     4081 agtcaagaat tatcaagcca atgccgaaga aattaaaaac agttttcaat attccgcgga
     4141 cagaagctgt tccaccgaaa gatatttggc ggcgagcact ttgaagaatc gaattattga
     4201 gttggagttc gcgttatcgc ggcttgacgg caccgcggtt gttcttgcgc cgacagtgtc
     4261 tgaaacgccg gtgaagccca atcccggatt gaatgtttcg ctgggattgg ttttgggatt
     4321 ttttgccggg attttcgcgg cgctggccaa aaattggtgg tctaatgcca aaaattaatg
     4381 atcaaaaatg agggcggaat ttgggtgctt ttggtcaaaa tcggaatttt cgcggcgttg
     4441 ttgacgccgg taatcgtgga ttttgatctc tattttcctt ttgtggcgtc aaagggattg
     4501 tggtttatgg cgtgtgccca gctggccttt ttttcgtggc tggttttggc atggcattgg
     4561 cgacattatc gcccgaattt caaaaatccg gtggtggccg ccgtgcttgc ttatttggga
     4621 gttttgtttt tgtcggcgat tttgggggcg gatttttccg cgagtttctg gtccactttt
     4681 gaaaggatgg gcggggtgct gatgttttgc catttggccg gatttatgat ttctgcggcc
     4741 ttggtgctgg aggcgcgcga ttggcgcaat gtttttttcg cctccgccgc ggtggcgctg
     4801 gcggtcggtt tgcgggcgct ttttgaccag tccgccgccg cgcgcggcgg cggatttttg
     4861 ggcaacgatt cgtttttggg aacctatctt ttgtttaatg tttttatcgc gctttatctt
     4921 ttttggtccg ggaaaaatgc cggtgccggc cggggagcgg cggttgcggg aattgttttt
     4981 gccgcgctgg cgttttgtct gcttgtccag ggaacgcaat tgtgggcggg gattatttcg
     5041 ggcggttttg ttttgccgga ggcgggaata ataaaagaca ttttcaattc cggcgcgcgc
     5101 gcggccaaaa tatcttttct gggcgggctg gttttgttgg aggggttgcg gttggttctt
     5161 ttggcaaaaa aaacgttgct gaaaaagatt gggatcattg ccgtctgcct tggcatcgcc
     5221 gccgccgcgg cggtttgggg atcgtttcaa aacggcggcg ccggcaaaca attttttacg
     5281 gacaattttg gagaaagcac cgttgccagc cgtttggcgg tttgggagat cgcctggcgc
     5341 ggttttttgg agcggccggt ttttggctgg gggccggaaa atttcggttt ggtttttgcc
     5401 cgccattaca atccttgttt gggctcatcc gcctgcggcg gcgcgacttg gtatgaccgc
     5461 gcgcacaata tcgtgtttga cactttggcc gaaacgggca tcgccggctt gatcgcttac
     5521 ctggcggttt ttgccgcggc gctgttcgtt ttgtggcaaa accgccgccg gcaaaacggc
     5581 attcctgccg ccgcggtttt cccggcgctg tttgccgctt attttgtcca aaacctgaca
     5641 gtgtttgata tgccggcgag ccatctgatg ctgtttttgt gcctgggttt tatcgcggcg
     5701 aattttgatc aaggcgggca aaaaccgaag accctttcgc ggccgcccga cgggcgcatt
     5761 ctggcggggg ttgctgtcgc tgccacaatc tgttttgcgg cgtttgtttt gtcgccgtgg
     5821 tcggcgtccg ccggcgtgat cggcgcgtca agagagccgt tcttttccgc cgccaaactt
     5881 gatttgcatc aaagagcgct ggaggcgtcg ccgctgggaa tccgccaggt ccggctgttt
     5941 tttgcccgcc aatggctcaa tgcgtccgaa gataaagaat ttattgaaaa agcgcgtgaa
     6001 ggggaaattc caatgggaga aatcaatcgt tacttcaatt tttttaccgg cgaattgaaa
     6061 aaaagcggcg cggattgcga atttgatttt gcttcccgtt tggaattggg gcggctctat
     6121 cgccaatgga gcgtgtttga tcgtgccaaa attccggcgg cggttgaggc gttggagagc
     6181 gcccgatcgc tttcgccgcg caaccaacaa cccttttggg aattggcgca gatgaagctc
     6241 tacgaaaata atgtcgccgg cgccgaagaa tcggcgcggc aagcccatga cctttatccg
     6301 gaaaatgttc agtcacgggc aattttggaa gagattcaaa aaatccgcga gaaccggcaa
     6361 taacgaatga tgcgatacgc cgttttcggt tcagagtttt gatttgcgtt gacaagaggc
     6421 aaaaatggcg ataaaatgac aatatatttc cgaaatatgt taaaaaacgg acgttacgga
     6481 aatataaaga gatgttatgc gatttattga attcaaggaa aagctaaagg ggtttaaaat
     6541 tttcagttta accgatattc gcaagaatga tcccgatttt gatttgcgac ggcttaatga
     6601 atggcaaaag aaaaagtata ttaaaaagat ccgcaaggga tggtatattt tttccgatct
     6661 tgaaatcaat gagtccggct tgtttttgat tgccaataaa atttacgcgc catcctatgt
     6721 ttcgctggaa atgggattgt cgcaatataa tttgattccg gaaatggttt atgaaataac
     6781 ttgcgttacc tcgcaaaaaa ccgcttcttt taaaaccggg atcgggaatt tttcctatcg
     6841 gcatatcaag ccggaattaa tgttcggtta tgaattgcgc cagcaaaacg attatttgta
     6901 tgccatcgcg ggaattgaaa aagcgttgct tgattatctt tatttaaaac caaaaatcaa
     6961 agaaaatgaa gattttcaag gaatgagatt taattacgat gaattcaaag aaagggcgga
     7021 tatgaaaaag tgggaaaaat atctgacggc gtttggaaat aaagccctaa ccgtccgcgc
     7081 caagaaattt attaaatatt tcaatgctta atctgtcgca aataacggaa tattattccg
     7141 cgccggaaag aaaattcaag cgtaatattt tgcgcgagta tttgcaatat aaaatattgg
     7201 aaatcatttt taattccaag gcgggacaga agatgtcttt tttgggcgga actgcgttgc
     7261 gtattgttta tgggagccct cgtttttcgg aagatttgga ttttgacaat ttcagcctca
     7321 cggaaaaaga ttttgccgag cttgctgata aaatccgcgt tgatttggca aagcaaggat
     7381 atcgggttga aataaaaaat attttcaagg gcgcgtatcg ctgttatgtg aaactgcctg
     7441 aagttctttt tgacaatgaa ttgtccaatt tgcgccaaga aaaaattgtg attcaggttg
     7501 ataccgcacc gcatttattc gggtatgaca gggagctgaa aaatatcaat aaatttgatg
     7561 tgttcactca aatatacgca acacccgttg atattatttt gtcgcaaaaa atatatgccg
     7621 cgttgaatcg caaacgaact aaggggcgcg acttttacga tattgttttt cttttgtcgc
     7681 gaacgatgcc gaattatgaa tatttaaacg ccaaactggg cgtgaaaaac ggaagcgatc
     7741 ttaagaaaat gttgttatcc aaaaccgcgg attttgactt tgaaacaatg gccgaagatg
     7801 tgaagccgtt tttaacggat gatagcggtg ttaagcgggt taaattgttt ttgccctata
     7861 tcaagtcgct ggaaatgaaa tgatcatgat caaaaaaaac gaaccgttgg cgaatcactg
     7921 cacctatcgc atcggcgggc cggcgcgggt tttgtttttg gccgccaacg agcgggagat
     7981 tattgccggc attgaaaccg ccgaagaaaa tcgtttcaaa atgatgattt tggggggcgg
     8041 cagcaatatt cttttttccg atcgcggttt tgacggattg gtgttgaagc ttgccaacaa
     8101 ttttataaaa attgccgaag aaaaagaaga tcgcgcggtg gttttggcgg gtgccggcgc
     8161 gccgctggcg gcgctggtga agttcgcgct tgataattct ttggcgggga tggaatgggc
     8221 cgccggcatt cccggaacgg cgggcggcgc gatccgcggc aacgcggggg cgttcggccg
     8281 cgagatcggc gcggcggttt tgcgggtgag ggcgctggag atctccggca aaagcattgt
     8341 gccggcaatc gtcggaaaag aagattgcgc gttcggttat cgcgaaagcg ttttcaagcg
     8401 gaacaaaaat ttggtgatta ccgccgcgaa atttgttttg gaaaagggga atgcaaatga
     8461 aatcgcggac aagatcaagg aatgcgccga aaaaaaacgg atttcccagc cgctggattt
     8521 tccctcggcg gggtcggtgt ttaaaaaccc ggaaggtttt tttgccgcaa agatcattga
     8581 agactgcggc ttaaagggaa gatcggccgg cggggcgcaa atttcggaaa agcacgccaa
     8641 cttcattgtt aatcgcggca atgccaaggc ggatgacgtt ttggcgttaa tcgcgcaaat
     8701 caaggccgcc gccaaagaaa aattcgccgt tgatctgaaa gaagaaattg aaatcgtgta
     8761 aacaaaattc gaatatcgaa tatcgaaatt cgaaacaata tcgaaattaa aattttcgaa
     8821 ttattaaaac aggagatccg atttttgttt tgaaaattaa aatttgtttt gaatttcgga
     8881 tttcgtgctt cggatttcta tttttatttt ttagaaaata aaaatagagt tatccacact
     8941 tgacaaagtt tttctgcgtg aaaaaatgaa gataacgaaa gccgtgaaaa tttttttgac
     9001 aaaaatttcg cgggcgaatt tcaaacggtc gaagagatca aaggaatttt aaaaataaaa
     9061 aaataaaaaa aatggcaacc aagaaaaaag ccgcgccgaa gaaaaaggcg gtcgcgaaaa
     9121 agaagcccgc ggcaaagaaa aagaagaggt agtttttgcg cttcgggagg aaaacaaaaa
     9181 aagctttaat taaaaattta tgccggtagt tccgaaacag aaaaaatcgc gcccgtcaaa
     9241 agaaaacaag atcaaggcgc gcaagagcaa aaagaaagtt tgcaatcgat aattaaagcg
     9301 ttcaaacaac aaaaggcata tttcaaaaga atatcctttt gttgttttag gagccttttt
     9361 taaaaaacaa tgaatattat cgtcaaaacc aaaaattttg agctcacccc gtctttgcgg
     9421 aaattcgccg aagaaaaaat ttcttcgctg aaaaaatatt ttgatttttt ccaaaccgaa
     9481 gacgcgccgg ggctcaaatc caaaattgac gcggccattg agttgggaaa aaccaccgcc
     9541 ggccagcgca agggcaacat tttccgcgcc gaggttttga taacttttca ccggaacaaa
     9601 ctgcgcgccg gcgtgagcgc cgacgacatt gagaaagcga ttgtgggcgc gcgcgacgac
     9661 cttcagcgcc aaatcaccga cttcaaagaa aaactccttg acaaaacccg caagtaaggg
     9721 taactatgga ctaaacatga ggtgaagtaa tgaagataag cttggcgtcg gggttaattc
     9781 ccaaacagca gatagtctgc agaggcgaag caggaaaatg gggggaggaa gttagttgtt
     9841 gggactggct gctcttttcc tattttaaaa gaaaaatatg tatcggcgaa aaaagacaca
     9901 aaggctggag aggtggcttg ccgcactata ttattttttg taagaagcac ggacccgtgg
     9961 tcacttatcc acaaggatac gctgaacaca tagagtgccc cttttgctga tcggggcttt
    10021 ctttttttat agattcggct acaataaggg ggcataaatc caattaccaa ttttcaatta
    10081 ccaattttca ataaatggca aaatgatgga attttcaatt ttggaatttt aaaattctga
    10141 catttattgg aaattggaag tttgacatta tgtcatttct tactcggatt ttcggcgacg
    10201 aaaacaaaaa ttatttcaaa aaactcaaac cgctggcgga aaagatcaat tctttggaaa
    10261 aagatcttcg ggatttggat gatggccgcc tgaaggccaa aaccgccgga ttcaaagagc
    10321 ggctggaaaa aggggagacg cccgatgatt tgttgccgga agcgttcgcg gcaacccgcg
    10381 aggcggcgcg ccgcactttg ggccagcgcc actttgacgt gcagatcatc ggcggcatcg
    10441 ttttgcatca ggggaggatc gccgaaatga agaccggcga aggcaaaact ttgaccgcca
    10501 ccgcgccggt atatttgaac gcgatttcgg ggaaaggcgt ccatttggta accgttaacg
    10561 attatttggc gcgacgcgat tgcgtgtgga tggggcaggt ttatcacgcg ctggggttgt
    10621 caaccgcgtg catcaaccat gatcagtcgt ttttgtatga tccacaatac aaaaaagccg
    10681 atgaaaccga agacgcggtg cgcgaccggt tgggatcgtt tcgggtgtcg caggattttt
    10741 tgcgcccttg ttcgcgccgg gaagcctatg cggccgatat tacttatggc accaataacg
    10801 aattcggatt tgactacctg cgcgacaata tggcttatcg ccgggaagac aaggttcagc
    10861 ggggatttaa ttttgccatt gttgacgaag ttgattcaat tttaattgat gaagcgcgca
    10921 cgccgctgat tatttcggcg ccggatcagg aaagctccaa gtggtaccgg gattttgccc
    10981 gcatcattcc gcgcctgtcg gcgcaaaccg attacaatat tgacgaaaaa gaccgtgccg
    11041 cgactttgac cgaagacggc atcgctaagg tggaaaaaat aatggggatg gataacatct
    11101 atgaggaaaa gggcgttaaa tatctgcatc atttggaaca ggccctgcgg gcgcaggcgc
    11161 tttaccggaa agaccgcgat tatgtggtta aagacgggca aattgtgatt attgatgagt
    11221 ttacggggcg gttgatgccc ggccgccgct ggtccggcgg cttgcatcag gcgattgagg
    11281 caaaagagga agtggcggtg caggccgaat cgcttacgct ggcgtcaatc actttccaaa
    11341 acctttttcg gatgtataaa aaactggcgg gaatgacggg aacggcaatg acttccgccg
    11401 aggaatttga caaagtctac aatttggatt gcgtgccgat tcccaccaac aagccgcaag
    11461 tccgcgttga tctgccggac cggatataca agaccgaagc tgccaaattc aaggcggtgg
    11521 tggccgaaat caaggaacgg caccgcgccg gccagccggt gctggtgggc acccgctcgg
    11581 tggaaaaaaa cgagtatttg ggaatgttgt tggcgcggga aggcgtgaag cacgagattt
    11641 tgaacgccaa aaaccacgaa cgggaaggcg aaatcatcgc gcaggccggc cgtttcgggg
    11701 cggtgaccat cgccaccaat atggcggggc gcggcgtgga cattattttg ggcggcaatc
    11761 ctcccgatgc cggggaggcc gagaaagtgc gtggcgccgg cgggctgttt atcatcggca
    11821 ccgaacggca cgaagcgcgg cgcattgaca atcagttgcg cgggcgcggc gggcggcagg
    11881 gtgatccggg gacgacgcag tttttcattt ccaccgagga tgatctggcg cggattttcg
    11941 cgggcgacac gatgaaaaaa ttgatggaga tgctgaaagt tcccgacgat atgccgattg
    12001 agtcggggat ggtctccaag cgcattgagg aggcgcagac caaaattgaa ggttcgcatt
    12061 ttgacatccg caagcatttg ctggattatg acggcgtgtt aaacaaacag cgcgaagcga
    12121 tctacaaaaa aagggacgaa attttggcgc aggacgacga gagttttcgc gctttcgttt
    12181 tggatttggc gcaaaagggg agtgcgcgag agcaatatga aaagcgggag gaggagaacg
    12241 gacgcgaagc gatgctgcaa gccggccggt ttatcgccct gcgcacgatt gattttttct
    12301 ggcaggagca tctggaattg atggacaatg tgcgcgacgc cacgcgcctg cgggcctatg
    12361 gccagcgcga tccgctggtg gaatacaaaa gcgaagccaa ccgccagttt cgggttttgc
    12421 tggaaaccgt ggacgccaac attttgcaaa atttgctcaa cgtcacggtg aacaaaaacg
    12481 cgccggcggc atcccaatcc gtgccttctg ccggtgcgcc ccgccaatcc gcggccaaat
    12541 atgcgggtgc gaaccgcaac gatccctgcc cctgcggtag cggcaaaaaa tataagaagt
    12601 gttgcggcaa gtaatattga ctacacataa attatgtgta tcataaaatt atgagcacaa
    12661 ttaccaaaaa taacaaccat aaaagaattg atatcacgct cccgccggaa actttggttt
    12721 tgcttgaaaa aatatcccgc aaaggagacc ggagtcgatt gatagacgaa gcaattcgtt
    12781 tttatgttaa agagaaaaat cgcgccaaat taagaaaaga tttgaaagag ggtgcgatta
    12841 aaaatgcgaa attgaattta tcaattgctg aagaatggtc ttctttggaa aacgaggcat
    12901 ggccgaaact ttaacgccaa agcgcggcga gatctatttg gttggttttg atccggcgat
    12961 cggatcggaa atcaaaaaga ccagaccggc gcttgtgata caaaatgata ttgccaaccg
    13021 ttatagcccg gttgttattg tgtcggcaat cagctccaaa ttcggaccaa aaatttatcc
    13081 cacggaggtt tttctccaac cgccaatggg aaatctcaaa attccttgcg tggttaaatt
    13141 ggaccaaatt aaaacagttg ataaaaaaag attaatcaaa aagatcggtt gcttggatgc
    13201 cgaaatgatg attttagtga accgcgcggc ggcaatcagc ttggaattga ttgatattta
    13261 gccggagcag agaataatag aaaatgcggc gggtaaataa tatggaaaaa ttactacacg
    13321 aacccaaggc ggggattggg gtgatggttt taaaagacgg gaaagttttg ctggggaaga
    13381 ggaaaggggc gcatggcgcg ggggaatttg cgtttccggg cgggcatttg gaatatatgg
    13441 aatcttttgc cgattgcgca cgccgcgaaa ccgccgaaga gtgcgggatt aaaataaaaa
    13501 acattcgttt ccgatttttg gcgaacttga ccgaatacgc acccaagcat tacgtccaca
    13561 tcggattggc cgccgattgg gaaagcggcg cgccgaaagt tttggaaccg gaaaaatccg
    13621 aatcttggca ttggtacgaa cttgataatt tgccttgccc gctgtttaaa acttgcgtgc
    13681 tggcgattga aagcttccaa accggcgtca attattttga catcagttga aattgcatag
    13741 gtgacaccta tgcaattttt gtttgttaat aattggcttt acttttcggc cgatttgtgg
    13801 tatcaagagg aggttagcgg caactaaatt gatttcatcg ggaggcaaaa attcggtctt
    13861 gccttcgcga aacgcaaaac gtatatgaag aaaaatcacg gatgggcgtt gttgtttatt
    13921 ctggcgctgg cggcggtgtc ggcggctttt tgttttcccg gcgctgtcaa tcggggaatt
    13981 gccgcggtaa atgccaaact gccgtttgcc attccgccga tggcggaaaa acctttcaat
    14041 ttgggattgg acgtcaaggg cggagtgcgc ctggaatatc aggcggactt gaatcagatc
    14101 gcggaagacg atcgagcgga agtgatggaa ggggtgaagg atctgattga gcggcgcatc
    14161 aacagctatg gcgtggccga gccgcaaatt cagattatcg gcgaaaaccg tctggcggtg
    14221 gagcttcccg gcattgaaag cgtccagcag gcgattgaat ggatcggcgc cactccttgg
    14281 ctggaatttt ccgaaccgcg gccggaagaa gagacgcaaa aaattctgga caaaatcaag
    14341 gaaatggaag gtaaaacttc cgaagaaata gcgcagatat cggatcttga aacgcttttt
    14401 gacgaacagg gaaatctcaa aaatccgtat tttatgccca ccgagcttac cggaaaatat
    14461 ctcaagagcg cgcggtttga ttttgaccca aataccaatc ggcccgttgt ggaattggag
    14521 tttgacgaag agggagccaa aatttttgaa caaatcaccg agcgcaacgt gggcaaagcg
    14581 ctggcaatct atctggacgg atttgccatt attgatacca gcggcgacgg ccaaattgac
    14641 aatagcgatt tatacgcgcc caatgtgaac gaaaaaattt cgggcggcaa agcggtgatc
    14701 acgggcattg ccggcacgca agaggccaaa actttggtga aacgattcaa cgaaggcgcc
    14761 ctgcccgtgc cgctgggaga gcccatcagc cagcaaaaga tcggccccac tctgggagcg
    14821 gtgtcgctgg acaaaaccgt ggccgcggga ttgatcggct ttttggcaat cgcggtgttt
    14881 atgattttct attatcgttt gccgggattg ctggcgtcgc tggcgctggc gctctatggc
    14941 gtgattttgc tggcggcgtt caaactgatg ggagtgacga tgagtttggc gggcatcggc
    15001 ggcttcattc tttctctggg gatggccatt gacgccaaca ttctgatttt ctcgcggatg
    15061 aaagaagaac tggccggcgg aaaaagtctg actgcggcca ttgaggaagg gttcgtccgc
    15121 gcctggccgg cgatccgcga cggcaacttt accacgatcg cggtggcgct gatcctgttc
    15181 tttttgggaa caagcttcgt gaagggattt gccgccactt tgatctgggg catacttttg
    15241 tcaatgtatt cggcgattgt ggtgaccaga acgtttttga ggattttttc gggcacccgg
    15301 ctgggtaaag cggtttggct gtggaaaccc ctcttctaaa atttaaaatt aaaaaatcaa
    15361 aattaaaaat ttgggagttc tttttaagaa gaatttttgc aaagcaaaaa ttcaatacca
    15421 aaacttttca ttttgatgtt tacattttca atttccatga tttcttttat caagcacaaa
    15481 aatatattct ttgttctcac tttggcggca atcgccgccg cggtcgccgc aatttcggtt
    15541 tggggattgc gtccgggaat tgaattcacc ggcggcagtc tgacgattgt tgaatatcat
    15601 gacgcacggc cggagaccgg ggcgatccgc gaaaaagttc aatctttggg tttggccgac
    15661 gcgtcggttt acgccgtgga
//
LOCUS       DAOQWE010000006        15546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_4243_length_15546_cov_8.479311, whole genome shotgun sequence.
ACCESSION   DAOQWE010000006 DAOQWE010000000
VERSION     DAOQWE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 15546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15546
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_15546_cov_8.479311"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..550)
                     /locus_tag="PKK37_00575"
     CDS             complement(173..550)
                     /locus_tag="PKK37_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HNR80913.1"
                     /translation="MPEPINKILSEIEKKLKEERSTVEAALSKIAVPNKNVPGDWQSK
                     FPYGEGESGTASLERQADEVEEYATRLPLEKNMETKLMAIDAALAKIKQGTYGKCENC
                     GKQIPLERLKIFPEAPFCVDCQK"
     gene            complement(708..>3225)
                     /locus_tag="PKK37_00580"
     CDS             complement(708..>3225)
                     /locus_tag="PKK37_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80914.1"
                     /translation="KKRQEEEKKKAEEMAANSLPITLKKLLIKLEEKKAAESKKAAEI
                     PAQKIPLEQKRRILESKIAMIRKSELAIVEEKEREIETKAAAKRNELEKNPDRTKEKT
                     LKQSLWQIEDSRKEIEKQRWAVEDKINKIKIDAESVDRQIAEKTRQLEASEEEISRIS
                     GQEALVRFATVKIRAEEEILKIIGEKESLAPELAAAENRKNDIEKKLADLTEKENLIS
                     RRLNDIETEEARAKDLAQKREIEKSRWQANNDLKSIVEEKWSGEEKLNSAASGLKLVQ
                     EKTDSLDAKIESIQNKITATEIELEEKELPVHTLRDAIRQLLEENSVNFNPKIISRIV
                     QIGKNGGKPNGESKVAAEKTPLEKADEGKNAVAESAQPQANNAIQNSGTAKKEDDKPI
                     EKKPAAGTVLRAEKVLPKENSPAAANNNPPEGKTETSSKKEPPNEAKTDLPKPTVAPA
                     TAATVAAKDAAGQKPFDEIKAPTSEEIAKMAEHNGSVYREQVEAAPARNIRTFDQENL
                     PKTAVIIGETKKNMPPAEETTIAGIKPIKPAETEPEISGDNFAANPENRWQQIKKTTL
                     PIASIPPKISLEDDSAPIIPEEATGSKKSNLLARILIVSAVIAVLGIVLAVILIKNGG
                     EAPAVVKNVPQTGTPAGGDPPEESAPAAIVTTISTVTIYVDNIANIPSSISPYMRKKF
                     DANGYHALSIIDKSTGKKIGLKQFFNAYKVSAPSVLYNSVKDDCEFFIYANNGKNRIG
                     FAAGITNAAMIEPAMAGWEGSIIKDTENLFRLLGRKTLDESGTFSFADGGTASATYKI
                     MDFAPPEDEFAIAWANYRQTYFIFSTSNNSMAKIFNQLPK"
     assembly_gap    3226..3325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3326..3870)
                     /locus_tag="PKK37_00585"
     CDS             complement(<3326..3870)
                     /locus_tag="PKK37_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80915.1"
                     /translation="MLKIGSDAIKTLAKTVARTKEKTPKDFLTETESPEITYFNRKLR
                     EAFGLFTVPQNMPLPAGKETKNNIKTPEPAIPEPAAEEIAKKSEKEKLQHEKEIKEKT
                     EEELARRREEAAKKAEEEKKRQEEEKKKAEEKKRQEEEAARKAEEEKARQLAALEAER
                     AKKAEEELARRREEAAKKAEE"
     gene            complement(3991..6099)
                     /gene="pnp"
                     /locus_tag="PKK37_00590"
     CDS             complement(3991..6099)
                     /gene="pnp"
                     /locus_tag="PKK37_00590"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNR80916.1"
                     /translation="MTSENKFSIQMGEKELSVEINGLADQANGSAVVRYGDTMVLTTA
                     VMAKKEKPDMGFFPLMVDYEEKYYAAGKIKGPRYIKRETRPSDEAVCNARQIDRSIRP
                     LFDDRLKNEVQTIATVLSWDAENEPDIISLFGISTALMVSDIPWQGPTASVRVGHLDG
                     KYVLNPTYEQRQASEIDVVFAGVERHGEILANMIEGGFNETEEAKILGAFAFAKPYLK
                     QLLDFQQKITTTCGKTKKTLLDRGPGEDLARELRNFIGEDLKNALYQPQKAQRGQEME
                     AAKAKLEEYAAQKYPDDAEIAKFIGKFYEKEIDRILHETILATGKRPDGRNPDELRPL
                     AAKTGVIPRTHGSGLFQRGETKALSAATLGAPGDQQLLDGMEITGKKRFLHHYNFPPY
                     SCGEVRFLRAPGRREIGHGMLAEKTLLPLIPSVEEFPYTIRVTSEIVSSNGSTSMAAL
                     SAASLALMDAGVPVKSHATGISMGLIAKDTQTYKILTDIQGPEDHHGDMDFKVAGTRK
                     GVNAIQMDVKIDGITEEMMGRVLEQGKKARHRILDVLEGELAAPRPELSPYAPRIIII
                     KINPDKIREVIGPGGKVINEIIDQTGVQIDIEEDGTIFITAEKPEAGQKASEWIQNIT
                     REVKVGEMFTGKVRRIMNFGAFVEILPGQEGMIHISKLADRRVNKVEDVVKIGDVVSV
                     KVVEIDDQGRINLSLKDAAK"
     gene            complement(6256..6759)
                     /locus_tag="PKK37_00595"
     CDS             complement(6256..6759)
                     /locus_tag="PKK37_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR80917.1"
                     /translation="MAVQLNAQRVAVLIDVQNLYYSARNLYQAKVNFREILKTAVGRR
                     GLIRAFAYVVRTKTGEEKPFFEALVKLGIETRIRDLQEYYDGLKKADWDVGITVDAIR
                     ISPSVDTIVLASGDGDFQQLIEYLENQGKRVEIIAFGRSASSKIKESADEFIDIEKEP
                     EKYLIKK"
     gene            complement(6779..7045)
                     /gene="rpsO"
                     /locus_tag="PKK37_00600"
     CDS             complement(6779..7045)
                     /gene="rpsO"
                     /locus_tag="PKK37_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNR80918.1"
                     /translation="MLTVEEKAKLIEKYKLHGEDTGSSAVQIALLSEEIDRLQKHLKR
                     HAKDMHSKRGLLKMVSKRRKFLNYLRDTDVHQYAQVLKKTGLKK"
     gene            complement(7140..7211)
                     /locus_tag="PKK37_00605"
     tRNA            complement(7140..7211)
                     /locus_tag="PKK37_00605"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7177..7179),aa:Lys,seq:ctt)
     gene            complement(7214..7618)
                     /locus_tag="PKK37_00610"
     CDS             complement(7214..7618)
                     /locus_tag="PKK37_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80919.1"
                     /translation="MKSVAAIFAAVLSGILFFSTAFAEQEPILPACGGAGQPACTICN
                     LFQLIDNLVRIVIVRLVPAAAGLVIAIAGFKFLVNQDNADVMEESKKIIFMVIIGLLL
                     IYGSYAIVGAIFSGMGYYDSMNPLEFNNLSCD"
     gene            complement(7667..7738)
                     /locus_tag="PKK37_00615"
     tRNA            complement(7667..7738)
                     /locus_tag="PKK37_00615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7703..7705),aa:Arg,seq:tcg)
     gene            7896..9386
                     /locus_tag="PKK37_00620"
     CDS             7896..9386
                     /locus_tag="PKK37_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR80920.1"
                     /translation="MDKFLDKIPQAVKNLIETAQSAGFECYIVGGCVRDLLAGAVPSD
                     WDAATNANPEQIQKIFPDSYADNKFGTIKVRAVSSSAAPMEIEITPYRVESKYTDKRH
                     PDTVEWADNISDDLSRRDFTVNAMALSYTKESGRAKVELMDPFGGQKDLRAKIIRAVR
                     DPEERFLEDALRMMRAVRFAATLGAEWKIEENTRRAVAKHSGWMKAVSGERIRDEFLK
                     IIMSPSAARGIELLRELKLLEHIIPELLEGYGCGQNKHHIYDCYHHLIKSLDYAAKKN
                     FGMHVRMASLLHDIGKPAAKSGDGPDATFYNHEIIGARFAKKILQRLKFSKKDADKIV
                     LLVRYHLFYYNVGEVSESSVRRLVKNVGRQNLEELLQVRMADRIGSGCPKAEPYKLRH
                     LRYLMERVAQDPISPKMIKVNGKDIMETLKIAPGPEVGQILDVLLGEILEDPARNDRD
                     CLIKRINELGALDDGKLSSLAAAAKQRRGQVEIKRDEMTKKRYWVV"
     gene            complement(10002..10895)
                     /locus_tag="PKK37_00625"
     CDS             complement(10002..10895)
                     /locus_tag="PKK37_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80921.1"
                     /translation="MAPNSNSRLPGFFPLIKQALEIYKQKWGTLITLFLLPFLAIILW
                     LLLIFAAGIGIKIAGAMTGISLPIWILFAASAVLFFLLLAPAWAWVQCAAIIAVKERP
                     AVETRESLRRAKPKILPYLLTVLPLYLAAGAGLMFFVVPGIVIGIWGFAGGYIAATED
                     KRGTAALAKSRGYVRGYFLKIFFLLAAEMFLSFLVNGIAESFGSNPIELLINFAFAPL
                     LFIFNYLIFEKLKQIKGDLPEPTAEQKNLFRIPAGIGLAIAISLVALAINFAPQIKQE
                     YEIRRLQYEAETKQELPPLPI"
     gene            11108..12112
                     /locus_tag="PKK37_00630"
     CDS             11108..12112
                     /locus_tag="PKK37_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR80922.1"
                     /translation="MTPKNSQLPNQSQESEDDYVLTLYEVCEMLGKSSRTITRYVQRH
                     ILHPRALKSCQGTLEYRFSKREIRRVRANEKNLRPYLFSEDDRPSPLPDYPSASFAAY
                     NYSPSMPAGASLPYLVPGVSFPMNQNQFHQVSPPAFFPSVAPSQNFAGEFMEKEMEFG
                     GRKNRAKDGSAKKALKKSEPEKIEKESGRNHASEEIVLLLKETTEMLRGQLKAKDDQI
                     KNLDDKIGQLIERNRETNILLKGLQDKIMLLEQPKIKRETRESSEINPPQAAAASPEI
                     RREEPAPAAESVAVVMSAVPSENPDSRRLPVPPERRNSGNNNNEDGAKKKGLFGKIFG
                     "
     gene            12281..13375
                     /locus_tag="PKK37_00635"
     CDS             12281..13375
                     /locus_tag="PKK37_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR80923.1"
                     /translation="MIKSFSLPPLPPKVDYEPLIRIIGEAHRAIGELNGLLRKNILAP
                     ELLAAPLLTKEAVLSSRIEGTQATLEDVFEYEAQGKTSQTGERERDIAEIINYRRAMN
                     FAIEELKIRPINQKFIQKIHYLLLDSSRGAGKDRGKFRTGQVYIGMPGAPIEDASYVP
                     PAPGDLPKLLENWERYVNTNQEKDSLAQIAVAHYQLEAIHPFRDGNGRVGRLLIPLFL
                     YQRDLLSSPLLYISEYFEKNRRDYYGHLRGVTELGDWESWLKFFFLALINQSLKTQNA
                     IVKIMELNARLKKEIIAVNSVHAPDLLDLLFSSPVISFAKIKDKLKTKNPQTIYNLLD
                     KFVQMKILFEEPGRQRNRTFTFKELLDILK"
     gene            complement(13581..13652)
                     /locus_tag="PKK37_00640"
     tRNA            complement(13581..13652)
                     /locus_tag="PKK37_00640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13617..13619),aa:Glu,seq:ttc)
     gene            13743..15284
                     /locus_tag="PKK37_00645"
     CDS             13743..15284
                     /locus_tag="PKK37_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR80924.1"
                     /translation="MEINYLNLARAEDLPGAKKAGYRFLEILPGLFAWGTLLFGFALS
                     WFWPLGVAIFIIAFDLYWLFRVLYLAAHQIASYRRMQKHIKTGWRAKLEADFSAQWRE
                     FYHLVVLPFAGESGEVVRATLDSLAKTDYPKENLIVLLAAEDMPSARRILEEAQKDYG
                     GRFFALWPSIHPKNISGELRGKGANVDWAIGVMQKKFLPATGIPKENIIVSFFDIDTK
                     PYADYFSCLAWHFLKSSNRLRESYQPIPVYNNNIWDAPWMSRIIATSSTFWEMMQQER
                     PEQLVTFSSHSMPLPALLEVGYPRNVVSDDSRIFWRAYFYYGGKYKTTPLYYPVSMDA
                     VLGKNTFRTMINQYKQQRRWAWGVENVPYVFFNLFFSPAAKKISLREKLFHVGTMLEG
                     FWSWATAALLTFGLAWLPLFLGGQNFGTTLLSYNLPRFTSVIMTMAMAGMMISAIISM
                     LLLPPRPKNYGRWRHFSMFLQWLLLPVTLIVFGAFPALESQTRMIINKPLGFWITEKG
                     GKGKK"
BASE COUNT         3505 a         3742 c         3769 g         4430 t
ORIGIN      
        1 attagttgcg cgtccaggcc ggtaatggcc gcggagcata tcttggaaag cacaaagtaa
       61 aatgtcaaaa ttcaaatctc aaatgtcaaa aacatgcgtt gccgttttga aattggaaat
      121 tgaaaattgc aaagtcatct tcgcggatac ccataaaaaa caaccttgcg gattattttt
      181 ggcaatcaac gcaaaagggc gcttccggaa aaatcttgag ccgttccagc ggaatctgct
      241 tgccgcaatt ttcgcatttg ccgtatgttc cctgcttgat tttagccagt gccgcgtcaa
      301 ttgccatcag cttggtttcc atattttttt ccagcggcaa acgggtggcg tattcctcca
      361 cttcgtcggc ttgccgttcc agcgatgccg ttcccgattc gccttcgccg taggggaact
      421 tgctttgcca atctccgggg acgtttttgt tgggaacggc gattttgctc agcgcggctt
      481 ccacggttga gcgttcttct tttaattttt tttcaatctc ggacagaatt ttatttattg
      541 gttccggcat ttgaatcgcg gccgtcgggc aattggtgaa tcgccggttt gggctagaaa
      601 ccaagcagaa tcccgcaatc gtccggtggc cggaggttat ttgggcgcgg cgaattctta
      661 aagaaaaatc gcctcgccgg ctttagtgcc attttatctt tctttcttta ttttggcaat
      721 tggttgaaaa tttttgccat ggaattgttg gaggtggaaa agatgaaata ggtttgccgg
      781 taatttgccc aggcgatggc gaattcgtct tccggcggcg caaaatccat tattttatag
      841 gttgcgcttg ccgtgccgcc gtccgcaaag ctgaaagtgc cgctctcgtc aagagtcttg
      901 cggcccaaca gccgaaaaag attttcggta tccttgataa tgcttccctc ccagccggcc
      961 attgcgggtt caatcattgc ggcgttggtg atcccggcgg cgaagccgat gcggtttttg
     1021 ccgttgttgg cgtaaatgaa gaattcgcaa tcatctttta ccgagttata aagaaccgat
     1081 ggcgcgctta ctttgtaagc gttgaaaaat tgtttcaagc cgatcttttt gccggtgctt
     1141 ttgtcaatga ttgacagcgc gtggtatccg ttggcgtcaa attttttcct catataaggg
     1201 gaaattgaac tggggatatt ggcaatattg tcaacataga ttgttaccgt ggagattgtc
     1261 gtcactatcg ccgccggcgc ggattcttcc ggcgggtcgc cgccggcggg agtgccggtt
     1321 tgcggaacat ttttaacaac cgccggcgct tcccccccgt ttttgatcag aatgactgcc
     1381 aaaacaattc ccaaaacggc aatgaccgcc gaaacaatca atattcttgc cagcaagttg
     1441 ctttttttgg atcccgtggc ctcttccggt attatcgggg cggaatcatc ttcaagggaa
     1501 atttttggcg gtatggacgc gattggcaga gtggtttttt taatttgttg ccatcgattt
     1561 tccggatttg ccgcgaaatt atccccgctg atttcgggtt cggtttccgc cggtttaatc
     1621 ggtttgattc cggcaattgt tgtttcctcc gccggtggca tatttttctt tgtttcgccg
     1681 atgatcaccg ccgtctttgg cagattttcc tggtcaaagg tgcggatatt tctcgccggt
     1741 gcggcctcca cctgctcgcg ataaacactg ccgttatgct ccgccatttt ggcaatctct
     1801 tcgcttgtcg gcgcttttat ctcatcaaaa ggtttttgcc cggcggcatc tttcgccgcc
     1861 actgttgccg ctgttgcggg ggcaacagtg ggcttgggta gatctgtttt tgcctcgttg
     1921 ggcggctctt tttttgagga tgtttccgtt tttccctccg gaggattgtt atttgccgcc
     1981 gccggagagt tttcttttgg aagaactttt tccgcgcgca aaacggtgcc cgccgccggt
     2041 ttcttttcta tcggtttgtc atcttctttt tttgccgtgc cgctgttttg gatggcgtta
     2101 ttcgcttgag gctgtgcgga ttccgctacc gcattttttc cttcatccgc tttttcaagc
     2161 ggcgtttttt cggccgccac cttgctctcg ccgtttggtt tccctccgtt cttgccaatc
     2221 tgcacgatgc gggagatgat tttgggattg aaattaactg agttttcctc caagagttgc
     2281 cggatagcgt cgcgcaacgt atgcaccggc agttcttttt cttccagctc tatttctgtg
     2341 gcggtgatct tgttctgaat ggattctatt ttcgcgtcaa gagaatccgt cttttcctga
     2401 accaacttca aaccgcttgc cgccgagttt aatttttctt caccgctcca tttttcttca
     2461 acaatgcttt tcagatcgtt gtttgcttgc catcggcttt tttcaatttc ccttttttgc
     2521 gccaaatctt tggcgcgcgc ttcttccgtt tcaatatcgt ttagccgccg cgaaattaaa
     2581 ttctcttttt ctgtcagatc ggcgagtttt ttttcaatat cattttttct gttctccgcc
     2641 gccgccaatt ccggagccag cgattctttt tctccgataa ttttgaggat ttcttcctcg
     2701 gcgcgaattt ttaccgtagc aaaacgcacc aatgcttctt gtccggaaat ccgggaaatt
     2761 tcttcctcgg aagcttccag ttggcgggtt ttttccgcaa tttggcgatc cacggattcc
     2821 gcgtcaatct tgattttatt gattttatct tccactgccc accgctgttt ttcaatttcc
     2881 ttgcggctgt cctcaatctg ccagaggctt tgtttcaggg ttttttcttt tgtgcgatcg
     2941 ggattttttt ccaattcgtt tctttttgcc gccgccttcg tttctatctc ccgctctttt
     3001 tcttccacga ttgccagttc gcttttgcgg atcatggcaa tcttgctttc caaaatcctc
     3061 cttttttgtt ccaagggaat tttttgagcg gggatttccg ccgccttctt gctttccgcc
     3121 gccttttttt cttcaagctt tataagaagt tttttgaggg ttatcggcag gctgtttgcc
     3181 gccatttctt ccgctttctt cttctcttcc tcttgccgct tttttnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnntcttc ctcggctttt ttagcggcct cttcccgccg
     3361 ccgcgccagc tcttcctcgg ccttcttggc gcgttccgct tccaacgccg ccaactgccg
     3421 cgccttttcc tcttccgctt tgcgcgcggc ctcttcctct tgccgctttt tttcttccgc
     3481 tttcttcttc tcttcctctt gccgcttttt ttcttcctcg gcttttttag cggcctcttc
     3541 ccgccgccgc gccagctctt cctcggtttt ttcttttatt tctttttcgt gttgcaattt
     3601 ttccttttcg gatttcttgg caatctcctc ggcggcaggt tccggaattg cgggttccgg
     3661 tgttttgata ttgtttttcg tttctttgcc ggccgggagc ggcatatttt gcggaaccgt
     3721 aaaaagaccg aaagcctcgc gcaactttcg attaaaataa gtgatttccg gcgattcggt
     3781 ttcggtcaaa aaatctttcg gcgttttttc cttcgtccgg gcaacggttt ttgccagagt
     3841 tttgatcgcg tcagatccaa tcttcagcat atgatttatt ctagcattat gcttttttcc
     3901 tgtaaaagcg gaatcgtttc gtccggccga aaaaaaattg gcgcaacaaa aaaacggagg
     3961 cgcctccgtt tttttgttgc gcccgcaaga ttattttgcg gcgtctttga gggaaagatt
     4021 gattcttcct tgatcgtcaa tctcaaccac tttcaccgaa accacatcgc cgatttttac
     4081 cacgtcctcc actttgttca cccggcggtc ggccagtttg gaaatgtgga tcattccttc
     4141 ctgtcccggc aaaatttcca cgaacgcgcc gaaattcatt atgcgtctta ctttgccggt
     4201 gaacatttcc cccactttca cttcccgggt gatattttgt atccattcgg aagctttttg
     4261 tcccgcctcc ggtttttcgg cggtgatgaa gatcgtgccg tcttcttcaa tgtcaatttg
     4321 cacgccggtt tggtcaatga tctcgttgat tactttgccg ccggggccga tcacttcgcg
     4381 gattttatcg gggttgattt tgatgatgat aatgcgcggc gcgtaaggcg agagttccgg
     4441 ccttggggcg gccagttccc cttccaaaac atccaatatc cgatggcgtg cttttttacc
     4501 ttgttccaaa acgcgtccca tcatttcttc ggtaatgccg tcaattttaa cgtccatttg
     4561 aatggcgttg acccccttgc gtgttccggc aaccttaaag tccatatcgc cgtgatggtc
     4621 ttccggtccc tggatgtcgg tgagtatttt gtatgtctgc gtgtctttgg cgatcagccc
     4681 cattgaaatg ccggtggcgt gcgacttaac gggcacgccc gcatccatca acgccagcga
     4741 cgcggcggaa agcgccgcca ttgaagtgga cccgttggaa gaaacgatct ccgaagttac
     4801 gcgaatggta tagggaaatt cttccaccga agggatgagc ggcagtaatg ttttttcggc
     4861 cagcattcca tggccgatct cgcgccgtcc cggcgcgcgc aaaaaccgca cttctccgca
     4921 ggaataaggc gggaaattat agtggtgcag aaatcgtttc ttgccggtga tttccattcc
     4981 gtcaagcaat tgctggtcgc cgggcgcccc cagggtcgcc gccgaaaggg ctttggtttc
     5041 gccgcgctgg aagagtccgg agccgtgggt gcgcggaatt acgccggttt tggccgccag
     5101 cggccgcaat tcgtcgggat tccttccgtc gggacgcttg ccggtggcca gaatcgtttc
     5161 gtgcaaaatg cggtcaatct ctttttcgta gaatttgccg atgaatttgg cgatttcggc
     5221 gtcatcggga tacttttggg cggcgtattc ttccagcttg gcctttgccg cttccatttc
     5281 ctggcctcgt tgcgcttttt gaggctgata aagcgcgttt ttcaaatctt cgccgataaa
     5341 attccgcaat tcccgcgcca gatcttcgcc ggggccgcga tcgagcaatg tttttttcgt
     5401 ttttccgcaa gtagtcgtaa tcttttgctg gaaatccaaa agctgtttga gataaggttt
     5461 ggcgaaggcg aacgcgccca gtattttcgc ctcttcggtt tcgttaaaac ccccctcaat
     5521 catattggcc agaatttctc cgtgtctttc cacgccggcg aagaccacgt caatttcgct
     5581 ggcctgccgc tgttcgtaag tcgggttcag gacatatttt ccgtcaagat gtcccacgcg
     5641 caccgaggcg gtcggacctt gccaaggaat gtcggacacc atcaacgccg ttgaaatacc
     5701 gaacaagctg atgatgtcgg gttcgttttc cgcgtcccaa gagagtacgg tggcgatagt
     5761 ttgcacttcg tttttcaaac gatcgtcaaa aagcggccga atggaacggt caatttgccg
     5821 ggcgttgcag acggcttcgt cgctggggcg ggtttcccgt ttgatgtagc gcggcccctt
     5881 gatcttgccc gccgcatagt atttttcttc ataatccacc atcaacggaa aaaaacccat
     5941 atcgggtttt tctttcttgg ccattaccgc ggtggtcaag accatggtgt cgccgtagcg
     6001 gacaaccgct gaaccgttgg cttgatcggc caagccgttg atttcaacgc ttaactcctt
     6061 ctctcccatt tgaatggaaa atttattttc gcttgtcata atttcgaata tcgaatatcg
     6121 aatatcgaaa caaattctaa ttttcaaaca atcaaatgat caaaacggat tcggagattt
     6181 tgttttgagc atttgaattt tgaaaattta aaaattgttt tgaatttcga atttcgtgct
     6241 tcggatttta taattttatt ttttaatcag atatttttcg ggttcttttt caatatcaat
     6301 aaattcatcg gcgctttcct taatcttgga agacgccgag cgtccgaagg cgatgatttc
     6361 cacccgcttg ccctgatttt ccagatattc aatcaattgc tggaagtcgc cgtctccgga
     6421 ggcaagcacg attgtgtcca cggacggcga aatgcggatc gcgtccacgg tgatgcccac
     6481 gtcccagtcc gctttcttca gcccgtcata atattcttga agatcgcgga tgcgggtttc
     6541 aattcccagc ttgaccagtg cttcaaaaaa cggcttttcc tcgccggttt ttgtgcgcac
     6601 aacataagca aacgcccgaa tcagcccgcg ccgtcccacg gcggttttca agatttcgcg
     6661 gaaattgact ttcgcttgat acaaattccg cgcggaataa taaagattct gcacgtcaat
     6721 caagacggcg acgcgctggg cgtttaattg gacggccatg gtttgagtcg ggcaaaaatt
     6781 atttttttaa tccggttttc ttcaaaactt gcgcgtattg gtgcacatcg gtatcgcgca
     6841 aataattcaa aaacttgcgg cgcttggaaa ccattttcaa caggccgcgc ttggaatgca
     6901 tgtccttggc gtgccgtttc aagtgtttct gcaggcggtc aatttcctca ctcagcaatg
     6961 cgatctgcac cgccgaagaa ccggtgtctt ccccgtgcaa tttatacttt tctatcaatt
     7021 tggctttctc ttcaacggta agcatttttt tattgattat tttctgcgga aaataaaatt
     7081 tattttaaat tattttaaat tattttaaat tttgattttt caatttttaa tttattatgt
     7141 gcccccagca ggattcgaac ctgcaacgaa ctgcttaaga ggcagctact ctaccgttga
     7201 gtcatggggg cggtcaatca cagctcaaat tgttgaattc cagcggattc atactgtcat
     7261 aatatcccat tcccgaaaaa atcgcgccga caatggcata actgccatag attagcagta
     7321 gcccgatgat caccataaag atgatttttt ttgattcctc catcacgtcg gcattgtctt
     7381 ggttcaccaa aaatttgaat cccgcgatgg cgatgaccaa cccggcggcg gccggtacca
     7441 atctcacgat cacgatccgg acaagattgt caatcaactg gaacaggttg cagattgtgc
     7501 atgccggttg tcccgcgccg ccgcaagcgg gcaatatcgg ttcttgttcc gcgaaagcgg
     7561 ttgagaagaa caggatcccg gacaaaactg cggcaaaaat ggcggcaacg cttttcattt
     7621 gtttttttgg tgtttgtttc ccgccgcgtt ttgttccggc cgggagcgcc tctggaggga
     7681 atcgaacccc cgtcaccggt tccgaagacc ggcactttat ccgttaagct acagaggcgg
     7741 gaaaaactca aatatcgctt ctatcctaac aaataattga aaaaaagtaa atattgcgat
     7801 aaagtctact tgaatcggcc ggcgcgtttt tgccgaacgc ggaatgccgg ccgccgcaag
     7861 aatgttaatt ttttagaatc ttatttatcg gaattatgga caaatttttg gataagatcc
     7921 ctcaagcagt gaaaaatctg attgaaaccg cccaaagcgc gggctttgaa tgctatatcg
     7981 tgggcggatg cgtgcgcgat cttttggcgg gcgcggttcc ttccgattgg gacgcggcga
     8041 ccaatgccaa tcccgaacag atacaaaaaa tatttcctga cagctatgcc gacaataaat
     8101 tcggcacgat caaagtccgg gcagtttctt catccgccgc gccgatggaa attgaaatta
     8161 ccccctatcg cgttgaatca aaatataccg acaagcggca ccccgacacg gtggaatggg
     8221 ccgacaatat ttccgacgat ctttcccgcc gggacttcac ggttaacgca atggcgttaa
     8281 gttacacaaa ggaaagcggc cgcgcaaagg ttgagttgat ggaccccttc ggcggccaaa
     8341 aagatttgcg cgcgaaaatt atccgcgcgg tgcgcgatcc ggaggaaaga tttttggaag
     8401 acgcgttgcg gatgatgcgc gcggtgcggt ttgccgcgac tctgggggcg gaatggaaga
     8461 ttgaagagaa taccaggcgg gcggtggcca agcattccgg gtggatgaag gcggtttccg
     8521 gcgaaagaat ccgcgacgaa tttttgaaaa ttattatgtc gccgtctgcc gcgcgcggca
     8581 ttgagctttt gcgcgaattg aaattgctgg agcatatcat tcccgaactt ttggaaggct
     8641 acggatgcgg gcagaacaag catcacattt atgactgcta tcaccatttg atcaaatctt
     8701 tggattatgc cgccaaaaag aatttcggta tgcacgtgag gatggcttcc ctcctccacg
     8761 atatcggcaa gccggccgcg aaatccggcg acggtcccga cgccactttt tataaccacg
     8821 aaatcatcgg cgcgcgcttt gccaaaaaaa ttctccagcg gctcaaattt tccaaaaaag
     8881 acgccgataa aattgtcttg ttggtgcgct atcatctttt ttattataac gtcggcgaag
     8941 tgagcgaatc gtccgtccgc cgcctggtga aaaatgtcgg ccgccaaaat ttggaagaac
     9001 ttctgcaagt ccgtatggcg gatcgcatcg gttcggggtg ccccaaagcc gaaccctaca
     9061 aattgaggca cctgcgttat ttgatggagc gcgtggcgca agatccgata tctcccaaaa
     9121 tgatcaaggt caacggcaaa gacataatgg aaactttgaa aatcgctccc gggccggaag
     9181 tcggccaaat tttggatgtg ttgttgggag agattttgga ggatcccgcg cgcaatgatc
     9241 gcgattgttt gataaagagg attaatgagc tgggcgcgct ggatgatggc aaattgtcta
     9301 gcctggcggc ggcggcaaaa cagcggcgcg ggcaagtgga gatcaagcgg gacgaaatga
     9361 ccaagaagag gtattgggtg gtttagcggg cgaaaacccg cgatgacgct ttttgcaacg
     9421 cgcataattt tctgctgaaa atttgctttc gctcggccgt cgccttcgcg gcattgcaaa
     9481 aagcgtcatc gcgggttttc ttattgatga gaattttgca acgcgcataa ttttctgctg
     9541 aaaattcgct tttgcgcgcc cgtcggctgt gcaacattac aagaagcaat agcgacaatg
     9601 tcttattggt tgatttgctt tcgctcggcc gtcgccttcg cttggggtct aacccctttg
     9661 taatcaaaag ggttagaccc ctctaaaagt attttgaatt ttggtgagta aaaatagaca
     9721 aaaggcggat tatgggttag gatggattgt gttgcgggac gatgagcgtc ccaaatgttg
     9781 cgctgattgt ggcggggagg agtatgccgg cagtacgcac gattcgggtt cgtgcagaca
     9841 gggttcgatt ccctgctccc cgacaaaaat taaaccccct taaattctca atcggcaaga
     9901 tggggttccg cacccgaggc gcgcagggat tccctgctcc ccgacaaaaa acaaaacgga
     9961 caaactgtcc gttttgtttt tcaagcttgg agatggcaag attatatcgg caagggcggc
    10021 agttcttgct tggtttcggc ttcatattga agccgcctga tttcatattc ctgttttatc
    10081 tgcggcgcga aattaattgc cagcgcgacc agcgaaatag cgattgccaa tccgatgccg
    10141 gcgggaatcc ggaaaaggtt tttttgttcc gcggtcggtt cgggcaaatc cccttttatt
    10201 tgtttcaatt tttcaaagat caaataattg aaaataaaca acaatggcgc gaacgcaaaa
    10261 tttatcaata attcaatcgg attgctgcca aacgattctg cgattccgtt gaccagaaac
    10321 gataaaaaca tttcagccgc cagcaagaaa aaaattttca aaaaatatcc gcgcacatat
    10381 ccccggcttt ttgccagcgc ggccgttccg cgcttgtctt ccgtggccgc gatatatccg
    10441 ccggcaaatc cccaaattcc gataacgatt cccggcacga caaaaaacat caagccggcg
    10501 ccggcggcca aatacagcgg caggacggtc aaaagataag gaagaatttt tggtttggcg
    10561 cgccgcaacg attctctcgt ttccactgcc ggcctttctt tcacggcgat gatggccgcg
    10621 cattgaaccc atgcccacgc cggcgccaac agcaaaaaaa acaggaccgc cgatgcggcg
    10681 aacaatatcc agataggcaa ggatattcct gtcatcgccc cggcgatctt tatgccaatg
    10741 ccggcggcaa aaatcaacaa cagccataat attatggcaa ggaagggcag taaaaaaagc
    10801 gttatcagcg tcccccattt ttgtttgtag atttccagag cttgttttat cagcggaaaa
    10861 aatccgggca atcgcgaatt tgaatttggt gccatttttt tgttgatttt gggttatccg
    10921 agttgataac ataatgatac attttttttg taaattttca aaatctgttt ttttggaaaa
    10981 gtaaattata aattattgga aagttgcaaa gggtgtttgg aggttttggc gcgtctgtct
    11041 ttgcaaaatt tgtgattatt ttctagaatc aaagcaacat ctccaaatta atgttccgat
    11101 aaaaaatatg acgccaaaaa attctcaact gccaaaccaa agccaagagt cggaagatga
    11161 ttacgttttg acgctttacg aagtctgcga gatgctgggc aaatcctcgc gcacgatcac
    11221 gcgctatgtc cagcgccata ttctgcatcc gcgcgcgtta aaaagctgcc aaggtacttt
    11281 ggaataccgc ttcagtaaaa gagaaatccg ccgggtgcgc gccaatgaaa aaaatttgcg
    11341 gccgtatctt ttttccgagg atgatcgccc cagccctctg cccgattatc cgtcggcaag
    11401 ctttgcggca tataattatt ctccttcaat gccggctggc gcgtccctgc cgtatctggt
    11461 tcccggagtg agctttccca tgaatcaaaa tcagtttcat caggtttcgc cgccggcctt
    11521 ttttccttcc gttgctcctt ctcaaaattt tgccggcgaa tttatggaaa aggaaatgga
    11581 atttggcggc cgcaaaaacc gggcaaaaga cgggtccgcc aagaaagcgt taaagaaatc
    11641 cgagcccgaa aaaattgaaa aagaatccgg ccggaatcac gccagcgaag agattgttct
    11701 gttgctcaaa gaaaccacgg agatgttgcg cggccagctg aaagccaaag acgaccagat
    11761 aaaaaatctt gacgataaga tcggccagtt gatagaaaga aaccgcgaaa ccaatatttt
    11821 gctcaaaggt ttgcaggaca aaataatgct tttggaacag ccgaaaatca agcgggaaac
    11881 ccgggagagt tccgaaatta acccgccgca ggccgcggcg gcctcgccgg agatccgtcg
    11941 cgaagaaccc gcgccggcgg cggaatccgt tgcggttgtc atgtccgccg ttccttctga
    12001 aaatcccgat tcccgccgat tgccggtgcc gcccgaaaga agaaattccg gcaataacaa
    12061 taatgaagat ggcgccaaga aaaaaggatt attcggcaaa attttcggat gacgcaacag
    12121 ttgctttgcg ttgttggttt ttttaaactt cggttttcgc cggagttttt cgttgaaccg
    12181 gttgagaaat ttccggcgat aatttagaat ggaataacgt tcgcggttat tccattgtga
    12241 tggaataacg ttcgcggtta ttccattgaa gcgaaacaat atgattaaat ctttttcatt
    12301 accgccgttg ccgccgaaag tggattatga acctttgatc cgcatcatcg gcgaagcgca
    12361 tcgggcaatc ggcgagctca acggattgtt gcgcaaaaat attttggcgc ccgaactgct
    12421 ggccgcgccg ctgttgacca aggaggcggt gttaagttcc cgcattgagg gaacgcaggc
    12481 gacgctggaa gatgtttttg aatatgaggc gcaagggaag acatcccaaa cgggggagag
    12541 agagcgtgat attgccgaaa tcatcaatta tcgccgcgcg atgaactttg cgattgagga
    12601 actcaagatc cggcccatca atcaaaaatt cattcaaaaa atccattatt tgcttttgga
    12661 ttcttcccgc ggcgccggca aggatcgcgg caaattccgc accggccaag tttatatcgg
    12721 tatgccgggc gcgccgattg aagacgccag ttatgtgccg cccgcgcccg gagacttgcc
    12781 aaaacttttg gaaaactggg agcgttatgt caacacgaat caagaaaaag attcgctggc
    12841 gcaaattgcc gtcgcgcatt accagttgga agcgattcat ccgtttcgcg acggcaacgg
    12901 ccgcgtgggg cgcttgctga ttccgctgtt tttataccag cgcgatttgc tgtcgtcgcc
    12961 gcttttgtac atcagtgaat attttgaaaa aaaccggcgc gattattacg gccatttgcg
    13021 cggcgtgact gaacttgggg attgggaatc atggctgaag tttttctttt tggcgctcat
    13081 caatcaatcg ctgaaaactc aaaacgcgat tgtcaaaatt atggaactga acgcccgttt
    13141 gaaaaaagaa atcattgccg ttaattcggt gcacgccccc gatcttttgg atttgctttt
    13201 ttcttcgccg gtgatttcgt ttgccaaaat caaagacaaa ttgaaaacca aaaacccgca
    13261 gaccatctac aatctcttgg ataaattcgt ccaaatgaag attctttttg aagagcccgg
    13321 ccgccagcgc aaccgcacct tcaccttcaa agaactgctg gatattttaa agtagcagta
    13381 attgtcctgt ctgctcgttt atcaaaaagc tttggcaagt gccaaagctt tttgactgct
    13441 gttaattttt gattaacatc ctttccttgc ccgtgatttt tttgcgattg caacggcgag
    13501 aggaaaagcg gtcttggctg ctacaacttt ctcgccctcc gtaattttaa cgaaggaggt
    13561 agtggaggag agagcgaggg tggccccaac gagaatcgaa ctcgtatttc cgccttgaaa
    13621 gggcagcgtc ctgaccatta gacgatgggg ccgaacacgc ggtatcatat cataaagatt
    13681 gccaaaagta aatcttggga gtatggtagt gcttgaaaga tatttttgtc tttgtcttaa
    13741 tcatggaaat taattatttg aatttggcgc gggcggagga tttgccgggc gcaaaaaaag
    13801 cgggctatcg ctttctggaa attttacccg ggcttttcgc ctggggcact ttgctgttcg
    13861 gttttgcgct gtcatggttt tggccgctgg gcgtggcgat attcatcatt gcctttgatc
    13921 tttactggct gtttcgcgtg ctttatctgg cggcgcatca aatcgccagt tatcgccgaa
    13981 tgcagaagca catcaagaca ggctggcgcg cgaagctgga agcggatttt tccgcgcaat
    14041 ggcgcgaatt ttatcatctg gtggtgttgc cgtttgccgg cgagagcggg gaagtggtgc
    14101 gcgccacttt ggactcgctg gccaaaaccg attatccgaa ggaaaatttg atcgtccttt
    14161 tggcggccga agatatgcct tcggcgcgcc ggattttgga agaagcgcaa aaagactacg
    14221 gcggccgttt tttcgccttg tggccatcaa tccatcccaa aaacatttcc ggagaattgc
    14281 gggggaaagg ggctaatgtt gattgggcga ttggcgttat gcaaaaaaag ttcttgcccg
    14341 caaccgggat cccgaaagaa aatattatcg tgtcgttttt tgatattgac accaaaccct
    14401 acgccgatta tttttcctgt ttggcgtggc attttttgaa gtcatctaat cgtctgcggg
    14461 aaagctacca gccgattccc gtctacaaca acaacatttg ggacgcgccg tggatgagcc
    14521 gaattatcgc cacctcttcc actttttggg aaatgatgca acaagagcgt ccggagcagt
    14581 tggtgacttt ttccagccac tcaatgcctc tgccggcgct gttggaggtg ggctatcccc
    14641 gcaacgtggt ttccgacgac tcgcggattt tttggcgcgc ctatttttat tacggcggga
    14701 agtataaaac cacgccgctt tattatcccg tgtcaatgga cgcggtgctg ggaaaaaaca
    14761 cttttcgcac gatgatcaac caatacaaac aacagcgccg ctgggcgtgg ggagtggaaa
    14821 acgttcctta cgtttttttc aatctttttt tcagtccggc ggcaaaaaaa atatctttgc
    14881 gggagaaatt attccatgtc ggcacgatgt tggaaggttt ctggtcgtgg gcgacggcgg
    14941 ctttgctcac tttcggattg gcgtggttgc cgttgttttt gggcggccag aatttcggca
    15001 ccactctgct ttcctataat ttgccgcggt tcacttcggt gattatgacc atggcgatgg
    15061 ccggaatgat gatttcggcg atcatcagta tgcttttact gccgccgcgg ccgaaaaatt
    15121 acggccggtg gcggcatttt tcaatgtttt tgcaatggtt gcttttgccg gtaacgctga
    15181 tagttttcgg cgcgtttccg gcgttggaat cccaaacccg catgatcatc aacaaacccc
    15241 tcggtttttg gatcacggaa aaaggaggca agggcaaaaa atagggctga tgttaagatt
    15301 aaactgataa ttactaacct tgactttaat aataataagg ttaaaattaa agtgttgtaa
    15361 tattttggct taaaactatg ttaatcaagc gggaaatttt tcaagaatta aaggaacatt
    15421 tgactcaaaa ggaaatttct ttgttggttg gtccgcggca agtcggcaaa accacattga
    15481 tgcgcgaatt agtcgcgcat cttgaaagta tcggtaaaaa gtatattttt ttaagtttgg
    15541 acaatg
//
LOCUS       DAOQWE010000007        15195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_4383_length_15195_cov_8.285535, whole genome shotgun sequence.
ACCESSION   DAOQWE010000007 DAOQWE010000000
VERSION     DAOQWE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 15195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15195
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4383_length_15195_cov_8.285535"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1486
                     /gene="dnaN"
                     /locus_tag="PKK37_00650"
     CDS             350..1486
                     /gene="dnaN"
                     /locus_tag="PKK37_00650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNR80925.1"
                     /translation="MKIKIPREKIKQAVNVAEKITGKDASLPILGNIMLKAEDNYLSV
                     IATNLETGIIWDLLAKTETAGSAVLPAGIFSGLVSSLPGSTLLIDKKNDTVSVVDEKT
                     KSNLISLAPEEFPTLPIIDGGDYFNVRADIFCQALTQVANFSGTSSVKPEINGVCVVI
                     ENDNIKIVATDSFRLGEKKISTAGKGLLSKKYSIILPARAVREIVAVFGNFQKNIIVY
                     LSDNQMMIDFSDDDGGGRSRIRFMSRLIDGEFPDYQAIIPDAHTTQIGFLKKEILFQL
                     KPAQIFSGKNSEITVEVSVKDKVAKIASRAADFGGYEGETGLLVAEGKDIAIVFNNRF
                     LIDGLSAITADECFLGFSGDDGPALLRSKTGDDFIYIIMPIKKY"
     gene            complement(1529..3466)
                     /locus_tag="PKK37_00655"
     CDS             complement(1529..3466)
                     /locus_tag="PKK37_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNR80926.1"
                     /translation="MSKFKSFFAKNGSKRKIGVFLGRFFAVIAVLALAGAVFAGMVFI
                     YYTRDLPRPEKFSEKNNIQSTKIYDRTGAILLYEIYGEEKRNWANLASIPDSMEKMAV
                     SAEDKNFYKNNIGIDIEGITRSFLTYFKTGRVTGGSTIPQQLIRSTFLTNEKTAERKI
                     REIVMAIELTRRYEKDDVLEWYLNQVPFGQNTYGVGAASANYFNKPVNELSLAQQATL
                     ISLIQSPSYYPAHFDDLMARKNYVLNRAAEDGYLSQQEAEDAKKETVSFIEKKIEIKA
                     PYFTLWIKQQLEEEYGRQYLEENGLTVITSLNWNMQQTAEQVVRDGVKNNYRYYAHNA
                     ALVALNPKNGEVLAMTVGTGDYYDNPYPENCSPGVDCLFDPQYNVAVMHPGRQPGSSF
                     KPFVYATAFEKGYSDTSIVNDTPTCFGKWGGIEYCPQNYTGTFQGPVTLRRALAGSLN
                     VPAVQVLNSMAGLNDSIAKAKEMGISTLNNPASFYGLALVLGGGEVTLLDMVSAYGIF
                     ANEGYRLPAAGIVDIKDSNGFSIYKNNKTPQKVLSQRSANLISDILSDNNARAYIFGA
                     NSPLYFPNHQVAVKTGTTSNYKDGWIIGYTPSIAAGVWAGNSNNAIIKGEPGSVVAGP
                     IFHTFMEKILASQPLEYFSKP"
     gene            3529..4728
                     /gene="tyrS"
                     /locus_tag="PKK37_00660"
     CDS             3529..4728
                     /gene="tyrS"
                     /locus_tag="PKK37_00660"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNR80927.1"
                     /translation="MEINTNPEKIAGIMSRGVFEAVDRRELEKKLASGKKLRVKLGID
                     PTAPDLHLGHAAVLRKLRQFQDMGHQAVLIVGDFTTLIGDPSGRSASRPQIDGAQIRD
                     NMKNYINQAAKVIDVESAEIRYNSEWFSPKPMLFLMDLAKRFTVARLIEREDFQKRLS
                     AGMDVSMLEMLYPLLQGYDSVAVSADVELGGYDQRLNILFGRKVQRSFDLPEQDAVFC
                     PLLPGIDGGKKMSKSAGNYIGLGEKPAEMFGKIMKIADASMWNYFEMLTGIGLPEIQK
                     LESDVESGKQHPRDVKMMLAHKIVSGFNGEGAADSAQKEFIRVAQEGDLPSDIFAAEV
                     KEKSLNIPDLLIALGAVKSKGEARRLCEQGGIKIDGVAKNDWQEMIGVKSGMVAQVGK
                     SKFFKVR"
     gene            complement(4914..5399)
                     /gene="ruvC"
                     /locus_tag="PKK37_00665"
     CDS             complement(4914..5399)
                     /gene="ruvC"
                     /locus_tag="PKK37_00665"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNR80928.1"
                     /translation="MIILGIDPGLATTGFGLVSPIGGKASKLKCVAYGTIDTKPEMAL
                     PHRLNKIHLDLNRVITQYRPKALVMENVFFFKNAKTIVPVSQAQGVILLAAAKKRLPV
                     YRYTPIEVKMTVTGFVKSDKKDVQREIQKILRLEEIPKPDDAADALSIAATYLIRSMN
                     L"
     assembly_gap    5512..5611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5899..6696)
                     /locus_tag="PKK37_00670"
     CDS             complement(5899..6696)
                     /locus_tag="PKK37_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR80929.1"
                     /translation="MNFENLKIASDFALRASCFNPMSGHSHAKTIAHAKGIADQKRGA
                     AFSKMVRLITVAVKEGGANPEINSRLRMALDQAKGINMPKDNIERAIARATGPGEGEI
                     FYDFLFEGYGPGNVALLVEGITDNKNRAVNDVKTILNQNNGKMVGEGAIRWMFERKGM
                     VIVKLEDQPESKQNKESLEMDAIEAGADDIYWFENILEVRTAPENAEAVKKTLEGKGI
                     KVESFSLAWMPKEEVEGDEKIKEQNEKLIEKLTDNDSVQEVYSNLKD"
     gene            complement(7081..8412)
                     /locus_tag="PKK37_00675"
     CDS             complement(7081..8412)
                     /locus_tag="PKK37_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNR80930.1"
                     /translation="MAIPQKLTQKITLPQGLIDSLKIDLLGLDEFRAKIQGTLANPDN
                     TVTQIIEIILAGGIVFDSSDVHIEGQENDAKLRVRLDGVLQDVVTFDKKTYEALVSRV
                     KLLSEMKLNVSNRPQDGRFSIAAGENSVEIRASILPTEYGDSVVMRILNPKSLIGLEE
                     AGLRKDLYDMFLEQIKRPNGMIIVTGPTGSGKTTTLYAFLKKLQSPEVKIVTIEDPIE
                     YHLEGISQTQVEPDKGYDFISGLRAIVRQDPDVILVGEIRDLETVDIALQAALTGHLV
                     FSTLHTNDAIGAIPRLISLGAKPFNIGPAINMAVAQRLVRRVCEKCAVKEPPSEEELA
                     EIKEGLAAAAADLAKKGVALPEIDNTLKVARAKGCPHCANTGYRGRVGIYEAFKVDDE
                     LQAYVLGTPTIPGIKEIVKRKGMLTLYQDGLIKVAQGITTLEELQRVAEKN"
     gene            8486..8947
                     /gene="tsaE"
                     /locus_tag="PKK37_00680"
     CDS             8486..8947
                     /gene="tsaE"
                     /locus_tag="PKK37_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNR80931.1"
                     /translation="MENKRQRYRVLSPVQTKKIGRLLAKEILRAGAGGAARVLALSGR
                     LGAGKTNFVQGFARGLGVKETVASPTFAIIKKYGLPPASGFKNFYHIDCYRLASGREL
                     AVLGAAKIFGDSANIVAIEWPQTAAGILPRETMAINFEVVGEQSRAIEFNF"
     gene            8976..9908
                     /locus_tag="PKK37_00685"
     CDS             8976..9908
                     /locus_tag="PKK37_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HNR80932.1"
                     /translation="MKNKKFVVIDGNSVVHRAFHAMPPLTNRKGEAVGAAHGFFLALL
                     RAIEDFRPDYLAVCFDTKGGTFREKSFAGYKAQRPATPPELIRQLETVQSLLVAAGVA
                     VFAMADFEADDLIASVVAAAKTESGVVDFFILTGDYDSLQLVDGRVKAFIVNRGIKNA
                     VIYDAAKVKEKFGVAPAQIPALKALAGDASDNIPGAPGIGPKAAVEILSKCGTLERAF
                     AEIETSPAGFCFGAGKRGENLKNILLRHKEQILDFQKLASMMADAPLGAVLGKCDFKN
                     FSAVRARAALLERGLAGIAKRLPAAGAGVNRTLF"
     gene            9994..11232
                     /locus_tag="PKK37_00690"
     CDS             9994..11232
                     /locus_tag="PKK37_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80933.1"
                     /translation="MNFIDFVNLFALAGKLLFFLANVVGLQLILWASAANWRTVFGRM
                     FLAGVLFVLLWMDADFLSSHAGVLFSSSAAMAAAGGFRAVYVLMAVFFAAFYVFALNF
                     PGANPLDVGRRHKGNFALALWAFLGVASLTPLVVREAVIGGTIPVAAWIVPGQLFWIY
                     VSAAMVSLALSFLELSRNRRFADAQNRGKVGPVAFSAATFGIFNLVFNIVAPALGEFW
                     GYVGFFAMPANYAIAILLGYVVYRAVHDKLFGIKIILVEVFVGLMGASLLALPIFLEL
                     LWQRALMLVLFVLFCIFGYILVQSTVKELREKEELELKVAQRTQELERAKMNLEELNA
                     VLEIRVRARTRELELLNQTLEEKIVARTNDLESKIKDLETFQKITVGRELKMIELKEE
                     NARLRAQISNPGENNYQNNG"
     gene            11225..12586
                     /locus_tag="PKK37_00695"
     CDS             11225..12586
                     /locus_tag="PKK37_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR80934.1"
                     /translation="MDKPIYSNSAPPPPAKLSPGDQSLRERELAELSEMKARLEEANG
                     VLEVRINAKTRALRELAANLEKEMKERTEELRRKVAESENSRVALMNMLEDMEDLRRR
                     AEEEREKTMAIIANFSDGLMFFDARDRLALVNPRIENYFSINKTESKNMIGGRISDIV
                     RFAPMSPLGEVFAGSLERVSRRELSLNEKTVFEVSVLEVAGRAGKIGTLLIIHDISRE
                     KMVEKMKTEFVSIAAHQLRTPISAIKWTLRMILDGDLGPINDEQREFLDKTYKSNERM
                     INLINDLLNVTRIEEGRQVYNSVVADIEEVVRGLIANYEDMARQKNLNLSFEKIAPVI
                     PTVKIDLEKIKLVASNLIENSLKYTPAGGKISVTLGEEDGNAMVEVIDTGIGIPAEQQ
                     DRIFTKYFRSANAIKAETEGTGLGLFIAKNIVEAHGGKIRFVSEPGRATKFSFVLPPA
                     ESD"
     gene            12637..13011
                     /locus_tag="PKK37_00700"
     CDS             12637..13011
                     /locus_tag="PKK37_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR80935.1"
                     /translation="MEKILLIEDEELIIRLLSKKLAAIGYDVSLAMNGEEGLEKIKQI
                     IPDLILLDIVMPRMGGFEVMAEMKKDEKIADIPVIIISNSGQPLELERAKELGAVDWL
                     VKTEFDPKEVAEKIQKYIRPTD"
     gene            13185..13553
                     /locus_tag="PKK37_00705"
     CDS             13185..13553
                     /locus_tag="PKK37_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR80936.1"
                     /translation="MAKKILIVEDDKFLRELIVRKISNEGYDVLEAVDGEQGLLQIKE
                     GHPDLVLLDLILPGIDGFEVLAQKREDPFMVSIPVIILSNLGQKEDVDKGLSLGAADY
                     LIKAHFTPGEIIEKVRNHLK"
     gene            13572..14486
                     /gene="holA"
                     /locus_tag="PKK37_00710"
     CDS             13572..14486
                     /gene="holA"
                     /locus_tag="PKK37_00710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNR80937.1"
                     /translation="MLIFLYGADTFRLRRKARQIISEYETRSGGADFAVFNMDESRAA
                     DLFAAFRQTSLFGAKRFFVVKNPISQKETKEALIAAAENIAAGPHNFLFLQEGKVLKT
                     DRFLKVIGKLGQAQEFAPLDGANLERWITAEFATLGHPEGQAIAPIMAAAIGNDLWRM
                     SGEIGKLVHFAAGRTISAADVAKVVSSVAENNIFQTVDAIAAGNKKEALRMVRRHAAT
                     GDHPLYLLAMIAAQFKNLLLAKRCAAAGARGLGMHPYVFSKSATLARRFDGARLEESY
                     GLIRQADFDIKTGKMTAAAGLDFLVAKL"
     gene            complement(14612..14878)
                     /gene="rpsT"
                     /locus_tag="PKK37_00715"
     CDS             complement(14612..14878)
                     /gene="rpsT"
                     /locus_tag="PKK37_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNR80938.1"
                     /translation="MPITKSAKKALRQNTKRRAENKTRKRSLKTLIKDARATAGQSDQ
                     GKKEAILPSIYKALDKAAKSGLIKKNSAARKKSRLTKLLAKKSA"
     gene            14976..>15195
                     /locus_tag="PKK37_00720"
     CDS             14976..>15195
                     /locus_tag="PKK37_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNR80939.1"
                     /translation="MKLKQLIKTAIKRVLPKSAIGAYHLVLTWAGAVWFWFPSRKIKV
                     IGVTGTNGKSTTAMMIATILSRAGFKTAV"
BASE COUNT         3706 a         3280 c         4068 g         4041 t
ORIGIN      
        1 gtgatgcacg cctacaaaaa aatctcttca aaaataaaag atgacgcatc tttctcggaa
       61 gaaataaact tgttaaaaaa taaaatctac agcgcttgat tttatttttg gggtgtgtaa
      121 aagctgttaa tcaacccccc aataaaaacc acaattaacc gccgataaaa aatggaaaaa
      181 taaaaacaaa aaaacccgcc ggcagtaaat cacatttttt ccacaaataa ttaaaccatc
      241 aatacaatta aacccgcaat cttaaaacga gcttaatatg ataattatcc accataataa
      301 ataatgataa tatttaaaat aattaataat taataataat taataaaaaa tgaaaattaa
      361 aattccacgg gaaaaaatta aacaagcggt aaatgtggcg gaaaaaatca ccggaaaaga
      421 cgcctcactg ccaattttgg gtaatattat gttaaaagcg gaagataatt atctttcggt
      481 tatcgccacc aatttggaaa ccggaataat ttgggattta ttggcgaaaa cggaaaccgc
      541 cggcagtgcg gttttaccgg cagggatatt ttccggttta gtttcttcat tacccgggtc
      601 aacattatta attgataaaa aaaatgacac cgtttcggtg gttgatgaaa aaacaaagtc
      661 aaatttaatc agtttggcgc cggaggaatt tccaacactt ccaataattg acggtggtga
      721 ttattttaat gttcgcgcgg atattttttg tcaagcctta acgcaagtgg ctaattttag
      781 cggcacatct tcggttaaac cggaaataaa cggcgtctgt gtggtaattg aaaacgataa
      841 tattaaaatt gttgccactg acagtttccg cctgggggaa aagaaaatat ccactgccgg
      901 taagggattg ttgtctaaaa aatattcaat cattttgccc gccagggcgg tgcgggaaat
      961 tgtggcggta tttggtaatt ttcaaaaaaa tatcatcgtt tatttatccg ataatcaaat
     1021 gatgattgat ttttcggatg acgatggcgg cggccggtcg cggatccgtt ttatgagccg
     1081 tttaattgat ggtgaatttc cggattatca agcgattatc cccgacgcgc acaccaccca
     1141 aatcggtttt ttaaaaaaag agattctctt tcaattaaaa ccggcgcaaa ttttctccgg
     1201 gaaaaacagc gaaataacgg tggaagtttc ggtaaaagac aaagtggcta aaattgccag
     1261 ccgcgccgcg gactttggcg gctatgaggg ggaaacgggg ttattggtgg cggaggggaa
     1321 ggatattgcg attgttttta ataatcgttt tttaattgac ggcttgtcgg cgatcactgc
     1381 cgatgaatgt tttttgggtt tttcggggga tgatgggccg gcgcttttac gatcaaagac
     1441 cggcgatgat tttatttaca taataatgcc gattaagaaa tattgatttt ttaaatcggc
     1501 gggccgtttg atgattgccg gcgcggagtt agggtttgct aaaatattcc aggggttggc
     1561 ttgccaggat tttctccata aaggtgtgga atattggtcc ggcaaccacc gaacccggct
     1621 ctcctttgat gattgcgttg ttactgttgc ccgcccatac gccggcggcg attgaggggg
     1681 tgtagccaat aatccaaccg tctttatagt tactggtggt gccggtcttg accgcgacct
     1741 gatgattggg aaaataaagc ggcgaattgg cgccaaaaat ataggcgcgg gcgttgttat
     1801 ccgaaaggat gtcgctgatg aggttggcgc tacgttggct caaaactttt tgcggggttt
     1861 tattgttttt gtaaatggaa aatccattac tgtctttaat atcaacgatt cccgcggctg
     1921 gcaggcgata accttcgttg gcaaaaattc cgtaagcgga aaccatatcc agcagggtaa
     1981 cttcgccgcc gccgagcacc agcgccaggc cgtagaacga cgccggatta ttaagggtgg
     2041 agatccccat ttctttggct ttagcgatgc tgtcgttgag tccggccatt gaattcagga
     2101 cctgtaccgc cggcacgtta agcgatccgg ccagcgcccg gcgaagggtt accggcccct
     2161 ggaaggtgcc ggtgtagttt tgggggcaat attctatccc cccccatttg ccaaaacacg
     2221 tgggggtgtc attgacgatg ctggtgtcgg aatatccttt ttcaaacgca gtggcgtaga
     2281 caaacggctt aaacgatgat cccggttggc gcccggggtg catcaccgcc acattatatt
     2341 gcggatcaaa cagacaatca actcccggcg agcaattttc gggataaggg ttgtcgtaat
     2401 aatcgccggt gcccacggtc atcgccaaaa cctctccatt tttggggttc agcgcgacca
     2461 gcgccgcgtt gtgcgcgtaa tagcggtaat tgtttttcac gccgtcgcgc accacttgtt
     2521 cggcggtttg ttgcatgttc cagttaagag aagtgatcac tgtcagtccg ttttcctcca
     2581 gatattgccg tccgtattct tcttccagtt gttgtttaat ccagagggtg aaataggggg
     2641 cctttatttc aatctttttt tcaatgaatg aaacggtttc ttttttggcg tcttcggctt
     2701 cttgttggct taaatatccg tcttcggcgg cccggttcaa cacatagttt ttgcgggcca
     2761 ttaaatcgtc aaaatgagcg gggtaataag atggtgattg gattaaagat attaaagttg
     2821 cctgttgggc cagactcagt tcgttgacgg gtttgttgaa ataattggcg ctggccgcgc
     2881 cgacgccgta ggtgttctgg ccgaagggca cctggtttaa ataccattcc aagacatcgt
     2941 ctttttcata gcgccgcgtg agttcaatgg ccatcacgat ctcgcggatt ttcctttcgg
     3001 cggttttttc attggtcaaa aaggttgacc ggatcaattg ttgggggatg gtggaaccgc
     3061 cggtgaccct gccggtttta aaataagtta aaaaggagcg ggtgattccc tcaatatcaa
     3121 tgccgatgtt atttttgtaa aaatttttgt cttcggcgga cactgccatt ttttccattg
     3181 aatccgggat ggaggcgaga ttggcccagt tgcgtttttc ttcgccgtag atttcataaa
     3241 gaaggattgc gccggtgcga tcgtaaattt tggtggattg gatattgttt ttttccgaaa
     3301 atttttccgg ccggggaaga tcgcgcgtgt aataaatgaa aaccattccg gcaaaaaccg
     3361 cgcccgccaa tgccaaaacg gcaatgaccg cgaaaaaccg tcccagaaaa acaccaatct
     3421 tgcgcttact gccgtttttt gcgaaaaaag atttgaattt tgacattttg gaatatttaa
     3481 attaaaaaac ctttgtgcgg atgatttatt attgtagaat gaaataatat ggaaataaac
     3541 accaacccgg aaaaaatcgc cggaattatg tctcgcggag tgtttgaagc ggttgatcgc
     3601 cgcgaactgg aaaaaaaact ggcgtccggg aaaaaattac gcgtcaaatt ggggattgat
     3661 cccaccgcgc cggatttgca tctggggcat gcggcggttt tgcgcaaatt gcgccaattt
     3721 caggatatgg gacaccaagc ggttttgatc gtgggcgact ttactaccct gatcggcgat
     3781 ccgtcgggga gatcggcgtc gcggccacag attgatggcg cgcagattcg ggataacatg
     3841 aaaaattata tcaatcaagc cgccaaagtg attgacgttg agagcgccga aatccgctat
     3901 aacagcgagt ggttttctcc caagccaatg ctgtttttga tggatttggc gaaaagattt
     3961 accgtggcca gattgattga gcgcgaagat tttcaaaaaa ggctgagtgc cggcatggat
     4021 gtgagtatgc tggaaatgct gtatccgctc ctgcaaggat atgattcggt ggcggtgtcc
     4081 gccgacgtgg aattgggcgg ttacgatcag cggctgaaca ttcttttcgg cagaaaggtc
     4141 cagcggtcgt ttgatttgcc cgaacaggac gcggttttct gcccattgtt gccggggatt
     4201 gacggcggaa agaaaatgtc aaaatccgcg ggtaattata tcggtttggg ggaaaaaccc
     4261 gccgaaatgt ttggcaaaat aatgaaaatc gccgatgcgt caatgtggaa ttattttgag
     4321 atgctcaccg gtatcggctt gccggaaatt caaaagttgg aaagcgacgt tgaaagcggg
     4381 aaacagcatc cgcgcgacgt taaaatgatg ctggcgcaca aaattgtttc gggctttaac
     4441 ggcgaaggcg ccgcagattc ggcgcaaaaa gagtttatcc gcgtggcgca agagggagat
     4501 ttgccgtcgg acattttcgc cgcggaagtc aaagaaaaat cgctcaacat tcctgatttg
     4561 ttgattgctt tgggggcggt gaagtcaaag ggagaagcgc ggcgattgtg cgagcaaggc
     4621 gggataaaaa ttgacggcgt tgctaaaaac gattggcaag aaatgattgg ggttaaaagc
     4681 gggatggtgg cgcaagtcgg caagagcaaa tttttcaaag tgagataatt gttgataacc
     4741 gtgtggacaa actgtttggc ggaagttgaa aatcttgttt aagaactatt gaaaaccgtg
     4801 ggataacccg cggttttgat tttttggcac ccgtaggggc gaatgattat tcgcccgtat
     4861 ttaatttaac gcaaaagggc gaataatcat tcgcccctac agataaaatt aaattacaaa
     4921 ttcatactgc ggataaggta agtcgccgcg atggacagcg cgtcggcggc gtcgtcgggc
     4981 ttggggattt cttccagccg cagaattttt tggatctcgc gctggacgtc ttttttgtcg
     5041 cttttgacaa acccggtgac ggtcattttc acttcaatcg gggtgtagcg gtagaccggc
     5101 aggcgttttt tggccgccgc caacaagatc acgccctgcg cttgcgatac cggcacgatg
     5161 gttttggcgt tcttgaaaaa aaagacgttt tccatcacca gcgccttggg gcggtattgg
     5221 gtgatgacgc gatttagatc caaatggatt ttgttgagcc ggtgagggag cgccatctcg
     5281 ggcttggtgt caatcgtgcc gtaggcgacg cactttaatt ttgacgcctt gccgccaatc
     5341 ggcgatacca gcccgaagcc ggtggtggcc agacccggat caattccaag aattatcata
     5401 aattttcaat tatcaatttc cagttttcaa taaattttca atgttttaat tttcaaaata
     5461 aaaagcaatt tcattatacg cccgcccgcc cctgttgccc accttgacaa annnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naatccttga aatttgccaa ttttgaggat
     5641 tttgggcgcg ggttgacaga aagtatcact aaatggctgt ccggcgatac aaaaagcaat
     5701 actttttgta tttttacggg gaggccgcaa aaaccgtttt catgaaatac gaaaatacaa
     5761 aaatgcgggc gggcataaag ccgcgcccct acgggtgcga aacgcgaatg caaaaaactc
     5821 cgcgcaagcg gagtttttga tcggcgatga tcaatgatca gtaataaatg ataagtaata
     5881 agtggtaagt ggtaagggtt agtcttttaa attggaatag acttcttgga cggaatcgtt
     5941 gtcggtgagt ttttcaatga gtttttcgtt ttgctctttg attttttcgt cgccctccac
     6001 ttcctctttg ggcatccacg ccagtgaaaa ggattccact ttgatgcctt ttccttccag
     6061 tgtttttttc acggcctcgg cgttttcggg tgcggtgcgc acttccaata tgttttcaaa
     6121 ccaataaata tcgtcggcgc cggcctcaat ggcgtccatt tccagcgact ctttgttttg
     6181 tttgctctcc ggctgatctt ccaatttgac gatcaccatt cccttgcgtt caaacatcca
     6241 gcggatggcg ccctcgccga ccatcttgcc gttgttttgg tttagaatgg ttttaacatc
     6301 gttgacggcg cggtttttgt tgtcggtgat gccttcaacc aaaagcgcca catttcccgg
     6361 gccgtagccc tcaaagagaa aatcgtagaa aatctcgcct tctccggggc cggtggcgcg
     6421 ggcaatcgcg cgctcaatgt tatctttggg catattgatg cccttggcct gatccagcgc
     6481 catgcgcagg cgcgaattta tttcggggtt ggcgccgcct tccttgacgg cgacagtgat
     6541 caggcgcacc attttggaga atgccgcacc ccgtttttgg tcggcgatgc ccttggcgtg
     6601 ggcgatggtt ttggcgtgtg agtgtccgga catgggattg aagcacgaag cgcgaagcgc
     6661 gaaatccgaa gcaattttta aattttcaaa attcaaatat ttcaaacaaa atgcaatgct
     6721 ttggttattc agattttgaa aattttgata ttgtttcgga tgttcggatt tcgatattcg
     6781 ggtttaactt gtcggttata caaaaaatta tcgcaaataa tatttgcgtt aacacaccca
     6841 tcctataaca aaaagcggtt ttggtcaatg gtagcgggcc ttgacaagaa aaccggaata
     6901 tactataatg cacgggtaat agaaaaaaca ccgttttgcc ggttttggcg acggttgaga
     6961 atctcgggat tgggagtctg gaatttttta cgatgggtgc tccggctgcg gcgggaacat
     7021 acctcgggaa attccaaaaa ttgggcgcag aaccaatttg actcccgatc ccgggatttt
     7081 ttaatttttt tcggcgactc tttgtaattc ttcaagggtg gtgattcctt gggcaacctt
     7141 aattagcccg tcctgataga gggtgagcat accttttctt tttacgattt ctttgattcc
     7201 cgggatggtg ggcgtgccca gcacgtaggc ttgcagttcg tcgtcaacct tgaaagcttc
     7261 atagatgccc acgcgtccgc gatagccggt gttggcgcaa tgcgggcatc ctttggcgcg
     7321 cgcgactttc agcgtgttat caatctccgg aagggcgact cctttttttg ccaaatccgc
     7381 cgccgcggcg gcaagcccct ccttgatttc cgccagctcc tcttcggaag gcggttcttt
     7441 gacggcgcac ttttcacaaa cgcggcggac caaacgctgg gcgacggcca tattgattgc
     7501 cggcccgata ttgaacggtt tggcgcccaa actgatcagg cgcgggatgg cgccgattgc
     7561 gtcattggtg tgcagggtgg agaacaccaa atggccggtg agcgcggctt gcaaggcaat
     7621 gtccacggtt tccagatcgc ggatttcgcc gaccaggatc acgtccgggt cttggcgcac
     7681 gatggcacgc aatccggaaa taaaatcata gcctttgtcg ggctccactt gggtttggga
     7741 aatcccttcc agatgatact caatcggatc ttcaatggtg acgattttca cctccgggct
     7801 ctgcagtttc ttcagaaacg cgtagagagt ggtggttttg cccgatccgg tggggccggt
     7861 gacgataatc attccgttgg gccgttttat ctgttccaga aacatatcat agagatcttt
     7921 gcgcaagccg gcctcttcca gcccgatcag gcttttcggg tttaaaattc tcatcaccac
     7981 cgaatcgcca tactcggtgg ggaggattga ggcgcggatt tccacggaat tttccccggc
     8041 ggcgatggaa aaccgcccgt cctgcggccg gttgcttacg ttgagtttca tttcggaaag
     8101 caacttcacg cgcgacacca gcgcttcgta agttttttta tcaaaggtga cgacatcctg
     8161 caaaacgcca tccagccgca cgcgcagttt ggcatcgttt tcctggccct caatgtggac
     8221 atcggaggaa tcaaaaacaa tgccgccggc caaaatgatc tcaatgattt gggtgacggt
     8281 gttgtcgggg ttggcgaggg ttccttggat tttggcgcgg aactcgtcca aaccgagcaa
     8341 gtcaattttc agcgaatcaa tgagtccctg cggcagagtg attttttggg tgagcttttg
     8401 agggattgcc ataaatgtgc aagaatagaa atgaagatga ttcttcaaat tatcccactc
     8461 tcggagaaaa gtcaattttt ttgccatgga aaataaacga cagagatacc gcgttttatc
     8521 gccggtgcaa acaaaaaaaa tagggcgcct gctggcaaaa gaaattttgc gcgccggcgc
     8581 gggcggcgcg gcgcgggtgc tggcgctgtc cggccggttg ggcgcgggta aaaccaattt
     8641 cgtccaggga tttgcgcgcg gactgggcgt caaagaaacg gtggccagtc cgacgtttgc
     8701 gatcattaaa aaatatggct tgcctcccgc ttccgggttc aagaattttt accatattga
     8761 ttgctaccgg ctggcttccg ggcgggagct ggcggttttg ggagcggcaa aaattttcgg
     8821 cgattcggcg aatattgtcg cgatagaatg gcctcaaacc gccgccggaa ttttgcctcg
     8881 ggagacgatg gcgattaact ttgaggttgt cggcgagcaa agccgcgcca tagagttcaa
     8941 tttttaattt cttcgcttcg gcggccggcc caattatgaa aaataaaaaa tttgtggtaa
     9001 ttgacggaaa ttcggtggtt caccgggcgt ttcacgccat gccgccgctc acaaaccgga
     9061 agggagaggc ggtgggggcg gcgcacggct tttttctcgc gctgttgcgg gcgattgaag
     9121 atttccgtcc tgattatctg gcggtttgtt ttgataccaa gggcggcacc ttccgcgaga
     9181 aatcgtttgc gggatataag gcccagcgtc cggcgacgcc gccggaattg attcgccagt
     9241 tggaaacggt gcaatcgtta ctggtcgccg cgggcgtggc ggtgtttgcg atggcggatt
     9301 ttgaggctga cgatctgatc gcgtcggtgg tggccgcggc aaaaacggag agcggggtcg
     9361 tcgatttttt cattttgacc ggagattatg atagcttgca attggtggac gggcgggtga
     9421 aggcgtttat cgtgaatcgc ggaatcaaaa acgccgttat ttacgacgcg gccaaagtga
     9481 aagaaaaatt cggcgtagcg ccggcgcaga ttccggcgct gaaagctctt gccggagacg
     9541 cttcggacaa cattccggga gcgccgggga tcggccccaa ggcggcggtg gaaattttgt
     9601 caaaatgcgg cacgctggag cgggcgtttg ccgaaattga aacttcgccg gccgggtttt
     9661 gttttggcgc ggggaagcgc ggagaaaatt taaaaaacat tcttttgcgc cataaagaac
     9721 aaattcttga tttccaaaaa ttggcgtcaa tgatggcgga tgctccgctc ggagcggtgc
     9781 tgggaaagtg cgactttaaa aatttttccg cggttcgcgc ccgcgccgcg ctgttggaga
     9841 gagggcttgc gggaatcgcc aagcgcctgc cggccgccgg ggcgggagtt aaccggacat
     9901 tgttttaatt ttttgcgccc gcgccggatt tttatgttat aataaaattg ttggaatcaa
     9961 ctcgttgaaa cggggttgcc gtttccagta aaaatgaatt tcattgattt tgtcaatctg
    10021 tttgcattgg ccgggaaact cctgtttttt ttggcgaatg tggtggggtt gcaattgatc
    10081 ttgtgggcct cggcggcgaa ttggcgcacg gttttcggga ggatgttttt ggcgggcgtt
    10141 ctctttgtct tgttgtggat ggacgccgat ttcttgagtt cgcatgcagg cgtgctgttt
    10201 tcctcttccg cggccatggc cgcggccggg gggttccgcg ccgtttatgt tttgatggcg
    10261 gtgttcttcg ccgcattcta cgtttttgcg ctgaattttc ccggtgccaa tcctttggat
    10321 gtcggccgcc ggcataaggg caattttgcg ctggcgttgt gggcgttttt gggtgtcgcg
    10381 tcgcttacgc cgttggtagt ccgggaagcg gtcatcggcg gcaccattcc ggttgcggcg
    10441 tggattgttc ccgggcaatt gttttggatt tatgtttccg cggcaatggt gtcgctggcg
    10501 ctttcatttt tagaattatc gcgcaatcgc cgctttgccg acgcgcagaa tcgggggaaa
    10561 gtcggtccgg tggcgttttc ggcggcaact ttcggaattt tcaatttggt tttcaatatc
    10621 gttgcgccgg cgctgggaga attttgggga tatgtcggtt tttttgcgat gccggcgaac
    10681 tatgccatcg ccattttact ggggtatgtt gtttatcgcg cggtgcacga caagcttttc
    10741 ggcatcaaaa tcattctggt ggaggtattc gtcgggttga tgggcgccag tttgttggct
    10801 ctgccgattt tccttgaatt gttgtggcag agggcgttaa tgctcgtttt gttcgttctt
    10861 ttctgtattt tcggttatat tctggtgcaa agcacggtta aggaacttcg ggaaaaagaa
    10921 gaacttgaat tgaaggtcgc ccaaagaacg caagaattgg aacgggcaaa aatgaatttg
    10981 gaagaattaa acgccgtttt ggaaattcgc gtgcgtgccc gcacgcggga actggaactg
    11041 ctcaaccaaa cgctggaaga aaaaatcgtt gcgcgcacca acgaccttga atcaaaaatc
    11101 aaagacttgg aaactttcca aaaaattacc gtcgggcgcg aattgaaaat gattgaattg
    11161 aaggaggaaa atgcgcgtct gcgcgcccaa atttcaaatc ccggagaaaa caactatcaa
    11221 aacaatggat aaaccgattt attccaacag tgctcctccg ccgccggcaa aactttcgcc
    11281 cggcgatcaa tctttgcgcg aacgcgaact ggccgaattg agcgaaatga aggcgcgcct
    11341 tgaggaggcc aacggcgttt tggaagttcg cattaatgcc aaaaccaggg cgttgcgcga
    11401 actggccgcc aatctggaaa aagaaatgaa ggagcggacc gaggaattgc ggcggaaagt
    11461 ggccgaatcc gaaaattccc gcgtggcctt gatgaatatg ttggaagata tggaagattt
    11521 gcgccgccgc gccgaagaag aaagggaaaa gacgatggcg attatcgcca atttttccga
    11581 cggattgatg ttttttgacg cgcgcgaccg tttggcgctg gtcaatcccc ggatagaaaa
    11641 ttatttttcc atcaataaaa cggaaagtaa aaatatgatt ggcggacgga tttcggatat
    11701 tgttcgcttc gcgccgatgt cgccgttggg agaagttttt gccggctcct tggaacgcgt
    11761 ttcccgccgg gagctttctt tgaacgaaaa gacggttttt gaagtgagtg ttcttgaagt
    11821 cgccggccgc gccggcaaga tcgggactct tttgatcatc catgacatca gccgggaaaa
    11881 aatggtggaa aaaatgaaga cggagttcgt ttccatcgcg gcccatcaat tgcgcacccc
    11941 gatttcggcg attaagtgga cactgaggat gattttggac ggcgatctcg gcccgatcaa
    12001 tgacgagcaa agagagtttt tggacaaaac ctataaatcc aatgaacgga tgatcaattt
    12061 gatcaacgat cttttaaacg tcacccgcat tgaggaaggg cggcaggtct acaattcggt
    12121 agtggccgat attgaagagg ttgtccgcgg cctgattgcc aactatgaag atatggcccg
    12181 ccaaaagaat ttgaatttgt cgtttgaaaa aattgcgccg gtcattccca cggtaaaaat
    12241 tgatcttgaa aagattaaat tggtggcctc caacctgatt gaaaattcgc tcaaatatac
    12301 gcccgccggc ggaaaaattt ccgtcaccct cggcgaggaa gacggcaatg cgatggttga
    12361 agtcatcgat acgggtatcg gaatccccgc ggaacagcaa gaccgtattt ttacgaaata
    12421 ctttcggagc gccaacgcga tcaaggcgga aaccgaaggc accggattgg gtttgtttat
    12481 tgccaagaat attgttgaag cccacggcgg gaagatccgc tttgtttccg agcccgggcg
    12541 ggccacgaaa ttttcttttg ttctgccgcc ggcggaatct gattgatggc gcaatttgct
    12601 tctttgggcg cggcgagtta taataacaga aaaggtatgg aaaaaatttt attgattgaa
    12661 gacgaggagc ttattatcag gcttttaagc aaaaaactgg ccgcgatcgg ttatgacgtg
    12721 tcgctggcga tgaatggcga ggaagggttg gaaaaaataa agcaaatcat ccccgatttg
    12781 attttgctgg acattgtgat gccgcggatg ggcggatttg aagtgatggc ggagatgaaa
    12841 aaagatgaaa aaatcgccga tatccccgtg atcatcattt ccaattccgg ccagccgctt
    12901 gaattggaaa gagctaagga attgggcgcg gttgactggc tggtgaaaac cgaatttgat
    12961 cccaaggaag tggcggaaaa gattcaaaaa tatatcaggc ctactgatta aatattttct
    13021 actgcaattt ccatatttcg gctacgccaa attcgtcagt cgtcggcaga ctttaagcca
    13081 agtatgcctc cttctttctc atttggctcg cgcgaaatct gaaaatttcg ttacgaaaat
    13141 atttaattag caggcctatc aacaaaaact aatccattct gcccatggca aagaaaattc
    13201 ttattgttga agacgataaa ttcctgcggg agcttatcgt gagaaaaatt tccaacgagg
    13261 gctatgatgt tttggaagcg gtggacggcg agcagggatt gttacagatc aaggaaggcc
    13321 atccggactt ggtgcttttg gatttgatct tgccggggat tgacgggttt gaagttttgg
    13381 cgcaaaaaag agaggacccg tttatggtgt caatcccggt gatcatcctt tctaatctgg
    13441 ggcaaaaaga agacgtggac aagggtctga gcctgggggc ggcggattac ttgatcaagg
    13501 ctcatttcac tcccggagaa atcattgaaa aggtgcgaaa tcatctcaaa taattctccg
    13561 cggcaagacg aatgttgatt tttctttacg gtgccgacac ttttcgtttg cggagaaagg
    13621 cgcgtcaaat tatctccgag tatgaaactc gctccggggg ggcggatttt gcggtcttta
    13681 atatggacga aagccgcgcg gcggatttgt tcgcggcatt tcggcaaaca tctttgtttg
    13741 gcgccaaaag gttttttgtc gtcaaaaatc cgatttccca aaaagagact aaggaggcgt
    13801 tgatcgccgc ggcggaaaac attgccgccg gccctcacaa tttcctgttt ttgcaggagg
    13861 ggaaagtttt aaaaaccgat cgttttttga aagtcatcgg caaattaggg caagcgcaag
    13921 aattcgcgcc gcttgacggc gcaaatcttg aaaggtggat taccgccgag tttgccactc
    13981 tggggcatcc ggagggtcaa gcgattgcgc cgatcatggc ggcggccatt ggcaatgacc
    14041 tttggcggat gagcggcgaa atcggcaaat tggtccactt tgccgctggc cgcacaattt
    14101 ccgccgccga tgtggcaaag gtcgtttctt ccgttgccga gaacaatatt tttcaaacgg
    14161 tggatgctat cgccgccgga aataaaaaag aagcgttgcg gatggtccgc cgccacgccg
    14221 ccacgggcga tcatcctctt tatctgctgg caatgattgc ggcgcaattc aagaatttgc
    14281 ttttggccaa acggtgcgcc gccgcgggcg cgcgcggact ggggatgcat ccctatgttt
    14341 tttccaaatc ggcgacactt gcccgccgtt ttgacggcgc gcgacttgag gagagctatg
    14401 gattaatccg gcaggctgac tttgacatta aaaccggcaa gatgactgcc gccgccggcc
    14461 tggatttttt ggtcgccaag ctttaagggg ctgttgccga ataggacttt cactgcaatt
    14521 taaatttcgt cagaaatcct gttcgtcaac agaccattga ttcgcaaaac aaaaacctct
    14581 ccatagctcc ggagaggttt tttggccgcc gtcaggcgga ttttttcgcc agcaatttcg
    14641 tcaggcggga tttcttccgc gccgcggaat tttttttaat caggccggat ttggcggcct
    14701 tgtcaagcgc tttgtaaatt gaaggcaaaa tggcctcctt tttaccctgg tcggattgcc
    14761 cggcggtcgc gcgcgcgtct tttatcagtg ttttcagact gcgtttgcgg gttttgtttt
    14821 cggcgcgccg tttggtgttt tggcgcaacg cttttttcgc ggatttggta atcggcatat
    14881 tttgaattaa aagttaaaag ctgaaaaata aatcacgaca ggttaaactg tatcataatt
    14941 tttgattttt gcaatacttt ggctataatg gcgatatgaa attaaaacaa ttgataaaaa
    15001 cggcgattaa aagagttttg cctaaatccg caattggcgc ttatcatttg gtgttaactt
    15061 gggcgggggc ggtttggttt tggtttccgt cgcgcaaaat taaggtaatc ggcgtcaccg
    15121 gcaccaacgg caaatcaact acggcgatga tgatcgctac gattttgtcg cgcgcggggt
    15181 tcaagactgc ggttt
//
LOCUS       DAOQWE010000008        11726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_5942_length_11726_cov_7.217205, whole genome shotgun sequence.
ACCESSION   DAOQWE010000008 DAOQWE010000000
VERSION     DAOQWE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5942_length_11726_cov_7.217205"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKK37_00725"
     CDS             <1..454
                     /locus_tag="PKK37_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNR80940.1"
                     /translation="AIWIGNKVMEAVAEDLDGRGQKTILSDLVFKKYKFKEINDLLKF
                     LYMDSSVDLPGLAPICDRAASRKDKIAQEILIAAGIEIARAVIAAAQRLDFDGQVPLV
                     LVGGVYKSRWVADTAANEIERRYPGKFDFVFAGDPVVGAVKLAIEAVK"
     gene            590..>1253
                     /locus_tag="PKK37_00730"
     CDS             590..>1253
                     /locus_tag="PKK37_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80941.1"
                     /translation="MNEAYFLAIETSCDDTSVSVLRAEEDGRGRLKKIEALSNVVSSQ
                     TELHAGYGGVYPFLAKREHQKNLPVVFEQAIKKIDVAKIKFIAATKGPGLDPCLWTGI
                     NFAQDLAKKYNLPLAGVNHLEAHFLANLLTLGIGEDEFAKNYLPAICLVVSGGHTLLF
                     LATGIGDYKLLGQTVDDAAGECFDKAARILGLGYPGGPEIAKLAQTYRGSTPVKLTGV
                     EPL"
     assembly_gap    1254..1353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1700..1948
                     /locus_tag="PKK37_00735"
     CDS             1700..1948
                     /locus_tag="PKK37_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNR80942.1"
                     /translation="MPRFDQTGPMGQGPRTGRGMGPCGRGYGRGGGYGFGCGMMGFGR
                     RRFISPRNEMAALEDEAEMLEEELKAVKEEIAALKSEK"
     gene            2052..2792
                     /locus_tag="PKK37_00740"
     CDS             2052..2792
                     /locus_tag="PKK37_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNR80943.1"
                     /translation="MATLLAIILATFSISLCVWAAVLFLYFKKETLDKITIFLVSLSA
                     GALTGGAFLHLLPEAAEKIPDDELYSIVLAAFVFFFFMEKLLFWRHCHKENCAIHTFG
                     YMNLIGDSVHNFIDGMVIAAAFLVNPGLGVATTLAIALHEIPQEIGDFGVLIHAGFGK
                     MKALATNYAIALMVVAGGVAGYFLSFAVENIIPYLLPFAAGGFIYIAASDLMPEIRKE
                     RVLKKSVGAFLVFLLGIAIMFAVKFIGE"
     gene            2802..3866
                     /locus_tag="PKK37_00745"
     CDS             2802..3866
                     /locus_tag="PKK37_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNR80944.1"
                     /translation="MKEKVALISVAANIFLAAAKVIAGAISGSAAVLADGFHSLADVF
                     SSGIAFLGIKISAKPVDERHPYGYYKAEVLAGAAITLILFVTGAGIIYGAWGEIAAPK
                     SIEMGGLALAAMAISAAVNEVMARLKIHFGKKENSVSLLADGIHSRVDIFSSLGVFAG
                     LLLAGAFPWADPLLAIIIGLYIIKESFSLGREAVDSLLDVSAGEEIEKQIKTVAARKN
                     IVLEKLKTQKKGAAITADLNIVLPAQTSVDEAAKISDRLRKKLMSAIDNLNYVAVEIK
                     SHGLETGFYKPEFGKGFGWQRQGRFKTDDGGQGRGPGGECVCPKCGRRAAHQSGVPCA
                     AIKCPDCNANMERDQAVNHQ"
     gene            3894..>4093
                     /locus_tag="PKK37_00750"
     CDS             3894..>4093
                     /locus_tag="PKK37_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNR80945.1"
                     /translation="MVRPRLCRNVKFNPQVNYFKPQGIPVRCLEVVQMSLEEMEALRL
                     KNLEGLEQTEAAQKMSTSQSTFA"
     assembly_gap    4094..4193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4194..5358)
                     /locus_tag="PKK37_00755"
     CDS             complement(<4194..5358)
                     /locus_tag="PKK37_00755"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR80946.1"
                     /translation="MKPRKIVPQLEDEIANPSKKIIFLWGPRQTGKSTLLNHLRQKHG
                     GAYFNFDDLNDQKLFVPELEKLRSVIAQKNGGKDSRIIFIDEIQNSPESTQSLKLLAD
                     RSDYIIFATGSSELRAKTRQFDTLAGRYKEFLLFPLTIDEIAIFHEDKISFEKSLSFA
                     NRDLLSRHLEENMIYGGYPAVILADNKIEEIKNITQNSVVKDIVNIYDLKNTDLIYNL
                     LRVLAMQIGNLINIAELASSLKTTTTTIDNYLSILAKNRIIYLLEPYRTNKRRGYLGR
                     KKVFFYDLGIRNALIDDFRALNLRSDLGAVFENLIIMGVLRQNIYERNNNKIYYFREI
                     SGSQKEIDLIIETPQGSKTGYEIKLHGGKPNIFPELQIAEYGTISKENAPDFLI"
     gene            complement(5537..5620)
                     /locus_tag="PKK37_00760"
     tRNA            complement(5537..5620)
                     /locus_tag="PKK37_00760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5584..5586),aa:Leu,seq:caa)
     assembly_gap    5680..5779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5780..>7761)
                     /gene="topA"
                     /locus_tag="PKK37_00765"
     CDS             complement(<5780..>7761)
                     /gene="topA"
                     /locus_tag="PKK37_00765"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNR80947.1"
                     /translation="PTKARTLQGFLGSGYKILSSFGHVRDLPKGDFGVDVDKDFEPKY
                     VIPTKARKNLTLLKKAAKDAGLILISTDPDREGEAIAWHLVEALKLDKPKTEKIEGKP
                     YQRIEFHEITKTAIENALKNPREINFDLVNAQQARRILDRLVGYKLSPFLWKKIAKGL
                     SAGRVQSVAVRLIAEREREIEAFKPEEYWEIAALLKNIENDPKKEFAARLNKINGRTL
                     DKLEIKDEPQAIAIEKNLAGAAYSIERIEKKETRKNPNAPFTTSTMQQAAWQRFKMTA
                     KAAMITAQQLYEMGLITYHRTDSLNLSAQSLAAAKSFIEKTYGKNYHPGHFNVYKTKS
                     KGAQEAHEAIRPAYPDQTPEMALADKDLSRPQAKLYELIWQRFVASQMAPAVFDSVSV
                     DIAAAGNSGTNYGFKSNGQTLKFDGYLKVYPSKFEQTELPAMETGAPLEFIKLNVDQH
                     FTKPPARYNEATLIKALEEQGIGRPSTYAPTISTIQTRNYIEKDERKSLKPTEIGLMV
                     NDLLVAHFPQIVDIGFTAKMEEEFDEIAEGRAEWVKVLREFYDPFIKNLEEKYQEVEK
                     KNKTPETTNKLCPKCGANLVIKMGRFGKFLACPKFPECKHAEPLAPEKPAFAAEIPCP
                     KCGAGHIVEKRTKKHKIFYGCSAYPACDFALWDKPTG"
     assembly_gap    7762..7861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7862..8715)
                     /gene="dprA"
                     /locus_tag="PKK37_00770"
     CDS             complement(<7862..8715)
                     /gene="dprA"
                     /locus_tag="PKK37_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNR80948.1"
                     /translation="MENDQIKKISEGDIEFPNCLRAIPSRPKQIYYRGNINIAAPCAA
                     IVGARVPSTYGKQAAHDIATGLARAGVCIVSGMAPGIDTIAHLAALECGGPTCGVIGA
                     GLDKESFFPQTNLGLAEKMVDAGGCLVSEYAPGFKSVYYSFPQRNRIIAGLSLAVIVI
                     EAKEKSGSLITAEWGRKQHKPVFAVPGSIYSTSSAGCNRLLKNGAAACTGAEDVLNFL
                     GLAGAGTKTTAKSKNPDEQKILDALRQGALNADKIIRAAALPAAAVMSLLPIMEINGA
                     IRNLGNGE"
     gene            8797..9114
                     /locus_tag="PKK37_00775"
     CDS             8797..9114
                     /locus_tag="PKK37_00775"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR80949.1"
                     /translation="MIKEKQINQITAKIIENHNPERIFLFGSLAWGKPRNDSDIDLLV
                     VKKNIKSMRQAAEDIDRTLFDRQVALDILVYSPEYFKKRLDLGDSFAKKIFDKGKLLY
                     AKK"
     gene            9101..9499
                     /locus_tag="PKK37_00780"
     CDS             9101..9499
                     /locus_tag="PKK37_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNR80950.1"
                     /translation="MPKNNFILAQEWFCLADNDELAAETLLKEGVAPSTVCFLSQQCA
                     EKYLKGLLAFLNMDVVKVHDLSQLVNLIKKSVPEIVNFTDEFVLLNKYYIQTRYLSDV
                     AEGFDCQEAKAALSAAKNVKKFVLDQIIPC"
     gene            9704..11008
                     /locus_tag="PKK37_00785"
     CDS             9704..11008
                     /locus_tag="PKK37_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR80951.1"
                     /translation="METNIKQLLRQIVESWQASELPAICDRETDLMDYFDPNLRKIIC
                     VTGFRRTGKTFSLFNFARKYGKDNCVYFNCEDERLPKETFVLTQLVEVLGEMYGQKSL
                     FLLVDEIQEIPDWSRWARRINETSRYRLILSGSSSKLSSAEIPTELRGRTVNVQMFPL
                     NWREYLKFKKIDLEKTPLEQKLYLLRDFLNFGGLPEIVLAQEGLRPMLLDDYYQTFVQ
                     RDIIGRERIRNKNALRDLLKLVLNSRFFTYSKFAASLKSLGHKIGKTTVIDYLRAAQN
                     AYFVFVLETGSPSVKKRLQSAKKIYSADTYFSARYGGVFSRNIGHLMEQAVCLEFLRR
                     CAHDGILEVFFWKDYRGREVDFLAMKNGQAMGLYQITYAETMAELPAREIESLLAAAK
                     TFSCGNLMVVTWGLEAEIEKDGQKIKFVPLSKFLSDPSFLKF"
     gene            complement(11070..11684)
                     /gene="pth"
                     /locus_tag="PKK37_00790"
     CDS             complement(11070..11684)
                     /gene="pth"
                     /locus_tag="PKK37_00790"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNR80952.1"
                     /translation="MEKKTMEKSPAVFIIGLGNPGDKYQNTPHNAGFLAVDEFARRFE
                     FPEFAMMPDGILVARKEIFGKTVILAKPQMLMNNSGKALAKLFNYLKIRPKKFRPDLW
                     VINDDLDIPLGKIKISQNRGAAGHKGVQSIIDQLKTKNFIRFRIGIASPIAQTKKIPA
                     DKYVLKKIPAADRTPFDQAIGTVISALSLALSDNPEKSMTEFNQ"
BASE COUNT         2765 a         2345 c         2995 g         3221 t
ORIGIN      
        1 ggcgatatgg atcggcaaca aagttatgga ggcggtggcg gaagatttgg atggcagagg
       61 gcaaaaaacg attctatcgg atttggtgtt taaaaaatac aaatttaagg aaataaacga
      121 tttattgaaa tttctctata tggattcgtc ggttgatttg cccggattgg cgccgatatg
      181 cgatcgcgcg gccagccgaa aagacaaaat tgcgcaagaa attttgattg ccgccggaat
      241 tgaaattgcg cgggcggtaa tcgcggcggc gcaaaggctt gattttgacg ggcaggtgcc
      301 gctggtgctg gtgggcggag tttacaagtc gcgctgggtg gccgataccg cggccaacga
      361 gattgaacgg cgctatccgg gcaaatttga ttttgtgttt gccggcgatc cggtggttgg
      421 ggcagtgaaa ctcgcgattg aagcggtaaa ataaatcaaa atgtcaaagc tcaaatttca
      481 aatcaaatcc aaagctcaaa tttcaaattg aattccctta tcccgttttg atatttgaca
      541 tttggatttt agattttatt tgtcatttgt cattttgatt ttgacattta tgaatgaagc
      601 atattttttg gcaattgaga cttcctgcga cgacacctcg gtgagcgttt tgcgcgcaga
      661 ggaggacggc aggggcaggt tgaaaaagat tgaggcgcta tcaaatgtcg tttcttcgca
      721 aacggaattg cacgccggat atggcggggt ttatccgttt ttggctaagc gtgagcatca
      781 aaaaaatctg ccggtggttt ttgagcaggc gataaaaaaa atcgatgttg ccaaaatcaa
      841 attcattgct gcgaccaagg gtccgggatt ggacccttgc ttgtggacgg gcataaattt
      901 tgcgcaggat ttggcaaaaa aatataacct gccgctggcg ggcgtcaatc atctggaagc
      961 gcattttttg gcgaatttac tcaccttggg aattggtgag gatgaattcg cgaaaaatta
     1021 cctgccggca atttgcctgg tggtttcggg cgggcacacg ctgttgtttt tagcaacggg
     1081 gatcggcgat tataaactgc tgggtcagac cgttgacgat gcggcgggcg agtgctttga
     1141 caaagcggcc cggattttgg ggctgggcta tccgggcggc ccggaaatcg ccaaactcgc
     1201 ccaaacttac aggggttcaa cccctgttaa acttacaggg gttgaacccc tgannnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatgcaaa aagcggccga tgaattggaa
     1381 atcagatttt tgtcgccggc ggttgatctg tcggtggata acgcggccat ggccggagcg
     1441 gcggggtatt ttgcttggaa gcggggcgaa attaccgccg accccgatga attggaatcc
     1501 cagccgaatt tggggatatg atcgctaatc attgatcatt tatttttaat ctttgacaag
     1561 aaatccgcga aagcgggttt tttgcttgac aagatgtcgt tatgaatata tattcaaaat
     1621 agaagaacga aaataaaggt cggaatgtga aagtgttaat caatttggaa tttccaaaaa
     1681 aaatttcaaa ttaaaaatta tgccgagatt tgatcaaacc ggaccgatgg gccaagggcc
     1741 gcgcaccggc aggggaatgg gtccttgcgg ccgcggctac ggacggggcg gtggttatgg
     1801 atttggatgc ggaatgatgg gatttggccg ccggagattc atttctccca gaaatgaaat
     1861 ggcggcgctg gaagacgaag cggaaatgct ggaagaagaa ttgaaggcgg tgaaagaaga
     1921 gatcgccgcg ctgaagagcg agaaatgatt gcggtagcaa aataacgccc ctttgctttc
     1981 aaaggggcgt tgtgttgatt aaaacaaatt ttggggatat tctataatca tcaattataa
     2041 actattgcaa tatggcgaca ttgttggcga ttattctggc gactttttcc atcagtttgt
     2101 gcgtctgggc ggcggtgctg tttttgtatt tcaagaaaga aacgctggac aaaatcacga
     2161 tatttttggt gtcgctttcc gccggggcgc tcaccggtgg tgcgtttttg catttattgc
     2221 ccgaagcggc cgaaaagatt ccggacgacg agctttattc catcgtgctg gcggcgttcg
     2281 tgtttttctt ttttatggaa aaattgctgt tttggcgcca ttgccacaaa gaaaattgcg
     2341 cgatccacac tttcggctat atgaacctga tcggcgattc ggtgcataat ttcattgacg
     2401 ggatggtgat tgccgccgcg tttttggtta accccggttt gggcgtggcc acgaccttgg
     2461 caatcgctct gcacgaaatc ccgcaggaaa tcggcgattt cggagtgttg attcacgcgg
     2521 gtttcggcaa aatgaaggcg ctggcgacaa attacgccat cgcgctgatg gtggtggccg
     2581 gcggggttgc gggatacttt ttgtcttttg cggtggaaaa tatcatcccg tatcttttgc
     2641 cgtttgccgc gggcggcttt atttatatcg ccgcttcgga tttgatgccc gaaatccgca
     2701 aagaaagagt tttgaaaaaa tccgtgggcg cgtttttggt gttcctgctg gggatcgcga
     2761 taatgtttgc cgtcaagttc atcggcgaat aatcaaattc aatgaaggaa aaggtggcgt
     2821 tgatttcagt ggcggccaat atctttttgg cggccgccaa agtcatcgcc ggcgcaatct
     2881 ccggatcggc ggcggtgctg gccgacggct ttcattcgtt agccgatgtt ttttcctcgg
     2941 ggattgcgtt tttgggcatt aaaatttcgg caaagccggt ggatgagagg catccctacg
     3001 gctattataa ggccgaggta ttggcgggcg cggcgatcac tttgattctt tttgtcacgg
     3061 gcgcggggat tatttatggc gcttggggag aaattgcggc gccaaagtca attgaaatgg
     3121 gcggattggc gctggcggcg atggcgatat cggcggcggt caatgaggta atggcgcgct
     3181 tgaaaatcca tttcggcaaa aaagaaaatt cggtgagttt gctggccgac ggcatccatt
     3241 cgcgagtgga tattttttct tcgctgggcg tgtttgccgg gttgctgttg gccggagctt
     3301 ttccttgggc ggatccgtta ttggcgatta tcatcgggtt atatattatc aaagaatcgt
     3361 tttcgctggg gcgcgaagcg gtggattctt tgctggatgt ttctgccggc gaggaaatcg
     3421 aaaaacaaat taaaaccgtc gcggcgcgaa agaatattgt gttggaaaaa ttgaaaaccc
     3481 aaaaaaaggg cgcggcgatt accgcggatt tgaatatcgt tttgcccgcg caaacgagcg
     3541 ttgacgaagc ggcaaaaatt tcggatcggc tgcgaaaaaa attaatgagt gcgattgata
     3601 atcttaatta tgttgccgtt gaaataaaaa gtcacggact ggaaactgga ttttacaaac
     3661 ccgaatttgg caagggtttt ggttggcaaa gacaggggag atttaaaacg gatgatggcg
     3721 gacaaggccg cggtccggga ggggaatgcg tttgtccgaa atgtggccgc cgcgcggcgc
     3781 atcaaagcgg cgttccttgc gcggcgatta aatgcccgga ttgcaatgcg aacatggaaa
     3841 gagatcaagc tgtgaatcat caatgataaa tgataaatga taaataaaaa aatatggtca
     3901 gaccaagatt gtgccgcaat gtcaaattca atccgcaagt aaattatttt aagccgcagg
     3961 gaattcccgt gcggtgcttg gaggtggtcc aaatgtcgct ggaagagatg gaagcgttgc
     4021 ggttgaaaaa tttggagggg ctggaacaga ccgaagcggc gcaaaaaatg agcacctcgc
     4081 aaagcacttt tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaattaa
     4201 aaagtcgggg gcgttttctt tggagattgt gccatattcg gcgatttgca gttcgggaaa
     4261 gatgtttggc ttgccgccat gtaattttat ttcatagccg gttttggatc cttgcggcgt
     4321 ttcaatgatc aaatcaattt ctttttgact gccggaaatt tcgcggaaat aatatatttt
     4381 gttattgttt ctttcgtaaa tattttggcg caaaacgccc attatgatca agttttcaaa
     4441 aaccgcgccc agatccgagc gtaaattgag cgcgcgaaaa tcatcaatca aggcgtttcg
     4501 aatgcccaaa tcgtagaaaa aaactttttt gcgcccaaga tagccgcgcc gcttattggt
     4561 gcgatatggt tccagtaaat aaatgatgcg gttttttgcc agaatgctca aataattatc
     4621 aatggtggtg gtggtggttt ttaacgagga agccagctcg gcgatgttga tcagatttcc
     4681 gatttgcatt gccaaaaccc gcaaaagatt ataaatcaag tcggtgtttt ttaaatcata
     4741 aatgttgaca atgtctttaa ccacgctgtt ttgagtaata tttttaatct cctcaatttt
     4801 gttatcagcc aaaatcaccg ccggataacc gccatagatc atattttctt ccaggtggcg
     4861 tgatagcaag tcgcggttgg caaatgagag cgatttttca aaactgattt tatcttcgtg
     4921 aaaaattgcg atctcgtcaa ttgtgagagg gaataagaga aactctttat aacgcccggc
     4981 gagagtgtca aattgccggg ttttggcgcg tagctccgat gaaccggtgg caaaaataat
     5041 ataatcggag cggtcggcaa gcagtttcaa cgattgggtg ctttccggcg aattttggat
     5101 ttcatcaata aaaataatgc gcgaatcttt cccgccattt ttttgcgcga ttactgatct
     5161 taatttttcc aattcgggga cgaacaattt ttggtcgttt agatcatcaa aattaaaata
     5221 cgcgccgccg tgtttttggc gcaagtgatt caaaagagtg cttttgccgg tttgtcgcgg
     5281 cccccacaaa aaaatgattt ttttggaagg attggcaatt tcatcttcta attgaggaac
     5341 aatttttctt ggcttcatat tttgttttta tatattcact atatgaaaaa catataaatt
     5401 agtcaagtgc ggtgcgctaa aaacaatgtt tttagcatag ttttggtaat tctgccccgt
     5461 attaacgata agcaataaac tattaactat taacaaaaat ccgccccctg gtggggagcg
     5521 gattttttga aaaaagtgcc gaaggtggga gttgaaccca cacgaggttg ccctcacagc
     5581 attttgagtg ctgcgtgtat gccaatttca ccacttcggc ggcaaatcat catttttgct
     5641 gatagtaaga tagcgcgaaa ttggggaaaa agcaagggan nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnc cggtgggttt gtcccagagg gcgaagtcgc aggcgggata
     5821 ggccgaacag ccgtagaaaa ttttgtgttt cttggtgcgc ttttccacaa tgtgtccggc
     5881 gccgcatttg gggcagggga tctccgccgc aaacgccggc ttctccggcg ccagaggttc
     5941 ggcgtgcttg cattcgggaa atttggggca ggccaggaat ttgccgaacc gtcccatttt
     6001 aatcaccaaa ttcgcgccgc atttggggca gagcttgttg gtggtttccg gcgttttgtt
     6061 tttcttttcc acttcttggt atttttcttc cagattttta ataaacggat catagaattc
     6121 gcgcaaaact ttcacccact cggcgcgccc ttcggcgatt tcgtcaaatt cttcttccat
     6181 cttggcggtg aagccgatat ccacgatctg cggaaaatgc gccaccagca agtcattgac
     6241 catcaggcca atctcggtgg gtttgagcga ttttctctcg tctttttcaa tataattgcg
     6301 ggtttggatg gtggagatgg tcggcgcgta ggttgacggc cggccgatgc cttgttcttc
     6361 cagcgctttg atgagggtgg cttcgttata tctggccggc ggcttggtaa aatgctgatc
     6421 cacattgagc ttgatgaatt ccagcggcgc gccggtttcc atcgctggca attccgtttg
     6481 ttcaaacttt gacggataga ccttcagata gccgtcaaat ttcaaggttt ggccgttgga
     6541 tttgaaaccg tagtttgttc ccgaatttcc ggcggcggca atatcaacgc tcacgctgtc
     6601 aaaaaccgcg ggcgccattt ggctggcgac aaacctttgc caaataagtt cgtataattt
     6661 ggcttgcggc cgcgaaagat ctttgtcggc cagcgccatt tccggagttt ggtcgggata
     6721 ggcggggcgg atggcttcgt gagcttcctg cgcgcccttg gatttggttt tgtagacatt
     6781 aaaatgtccc ggatggtaat tcttgccgta ggttttttca atgaagcttt tggctgcggc
     6841 cagcgattgg gcggataaat tcagcgagtc ggtgcggtga taggtgatca gtcccatttc
     6901 atacaattgc tgggcggtta tcattgcggc cttggcggtc atcttgaatc tttgccaggc
     6961 ggcctgttgc atcgttgaag tggtgaacgg cgcgtttggg ttcttgcgcg tttctttttt
     7021 ctcaattctc tcaattgaat aagccgcgcc cgccaaattt ttttcaatgg cgatggcttg
     7081 cggttcatct tttatttcca gcttgtccag cgttcggccg ttgattttgt taagccgcgc
     7141 cgcaaattct tttttggggt cattctcaat gtttttcaaa agcgcggcga tctcccaata
     7201 ttcttcgggt ttgaaagctt caatttcccg ctcgcgttcg gcgatcaggc gcaccgccac
     7261 cgactgcacc cgtccggccg acaatccctt ggcgattttt ttccacagga acggcgacag
     7321 cttatagccc acaaggcggt ccaaaatccg gcgcgcttgc tgggcgttga ccagatcaaa
     7381 gttgatttcg cgcggatttt taagcgcgtt ttcaattgcg gttttggtga tttcgtggaa
     7441 ttcaatccgt tggtagggct tgccttcgat tttttcggtt ttgggtttgt ccagtttcaa
     7501 cgcctccacc aaatgccagg cgatggcttc gccttcgcgg tccggatcgg ttgaaatcaa
     7561 aatcaacccg gcgtctttag ccgctttttt cagcaaagtc aaatttttcc ttgctttcgt
     7621 cgggatgaca tatttcggtt caaaatcttt atcaacatca acgccaaaat cgcccttggg
     7681 caggtcgcgc acatgtccga acgacgagag tattttgtag cccgacccca aaaacccttg
     7741 cagggtccgg gctttggtgg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 ntattctccg ttgcccaaat tgcggatcgc gccgttgatt tccattatgg gaagcaggct
     7921 catcaccgcg gccgcgggca gtgcggccgc gcgaatgatt ttgtcggcgt tcagcgcgcc
     7981 ctggcgcagg gcatccaaga ttttttgttc gtcgggattt ttgcttttgg ccgtggtttt
     8041 tgttccggca ccggccagtc ccagaaagtt caaaacgtct tcggcgccgg tgcaagcggc
     8101 cgcgccgttt tttaacagac ggttgcatcc ggccgaagaa gttgaataga ttgaccccgg
     8161 aacggcaaag accggtttat gctgttttct gccccattcg gcggtgatca gcgatcctga
     8221 tttttctttg gcttcaatga cgatcaccgc cagcgacagg ccggcgatga tgcggtttcg
     8281 ctggggaaag gaatagtaga cggatttgaa tcccggcgcg tattcgctca ccaagcatcc
     8341 gccggcgtca accatctttt ccgccagccc aaggtttgtt tgcgggaaaa acgattcttt
     8401 gtcaagcccg gcgccgatca cgccgcaggt tgggccgccg cattcaaggg cggcaaggtg
     8461 ggcgatggtg tcaatgcccg gggccattcc cgaaacgatg caaacgccgg cgcgcgccaa
     8521 tcccgtggcg atgtcgtgcg cggcctgttt gccgtaggtt gagggaacgc gcgcacccac
     8581 gatcgccgcg catggcgcgg cgatattgat gttgccgcga taataaattt gcttcggccg
     8641 cgaaggaatg gcgcgcaggc aattgggaaa ttcaatatct ccctcgctta tttttttgat
     8701 ttggtcgttt tccacttttt cactatacca ttttttcatc ccttgcaaaa aacggatatt
     8761 ggtaaaaact atggaaaatg atattatgtc tctattatga ttaaggaaaa gcagatcaat
     8821 caaattaccg caaaaatcat tgaaaatcat aatcccgaaa ggatatttct ttttggttct
     8881 ttggcttggg gaaagccgag aaatgatagc gacattgatt tgctggtcgt taaaaaaaat
     8941 atcaaatcaa tgcgtcaagc cgccgaagat attgaccgca ctttatttga tcgccaagtt
     9001 gcgctggata ttcttgttta ttcgccggaa tattttaaaa aacggctgga tttgggcgat
     9061 tcgtttgcaa aaaaaatttt tgataaaggc aaattgcttt atgccaaaaa ataatttcat
     9121 tcttgcgcaa gaatggtttt gccttgccga taatgatgaa ttggccgcgg aaacgctttt
     9181 gaaagaaggg gtcgctccaa gcacggtttg ttttttgtcg caacaatgcg cggaaaaata
     9241 tttaaaaggt ttgctggctt ttttgaatat ggatgttgtc aaagttcatg atttgtcgca
     9301 attggtgaat ttgatcaaga aatcggtgcc cgaaatcgtc aattttaccg atgaatttgt
     9361 tttgcttaac aagtattata ttcaaactcg ttatttatcc gatgtcgccg aagggtttga
     9421 ttgccaagaa gccaaagccg cgttaagtgc ggccaaaaat gtcaaaaagt ttgttttgga
     9481 tcaaataatt ccttgttgag gcatagagac caagcactat ccataaattg ttttaatttt
     9541 ttggaaagtt cgggcttttt tgtttttgaa acgcaaatgc aaaatatttt taaaaggcct
     9601 aactcctgcg gatacgcagg ggatagaccc caaaaatgca aattcattgg ttttgtgctt
     9661 tagggattgc attatagaac gatatttgat aatattgtcc tatatggaaa caaatataaa
     9721 gcaattattg cggcaaatcg tggaaagctg gcaagcaagc gaattgccgg caatttgcga
     9781 tcgggaaacc gatttaatgg attattttga ccccaacttg aggaaaataa tttgcgtgac
     9841 cggttttcgg cgcaccggta aaactttttc gctttttaat tttgcccgaa aatacggcaa
     9901 agacaattgc gtttatttca attgcgaaga cgagcgtttg cccaaagaga cgtttgtttt
     9961 aactcagctg gttgaggttt tgggggaaat gtatgggcaa aaatcgttgt ttttgctggt
    10021 ggatgaaatt caggaaattc ccgattggag ccgctgggcg cggcggatca atgaaaccag
    10081 ccgctatcgt ttaatcttga gcgggtcgtc ttccaaattg tcttcggcgg aaattcccac
    10141 ggaattgcgc gggcgcaccg tcaacgtgca aatgtttccg ctcaattggc gggaatattt
    10201 gaaattcaaa aaaattgatc ttgaaaaaac accgcttgaa caaaaactct atcttttgcg
    10261 cgacttcttg aattttggcg gattgcccga aattgttttg gcgcaagagg gattgcggcc
    10321 gatgcttttg gacgattact atcaaacatt cgtccaacgg gacattattg gccgggaaag
    10381 aataagaaat aaaaatgctt tgcgcgatct tttgaagctg gttttaaaca gccggttttt
    10441 tacttactcc aaatttgccg ccagcttgaa gagtttgggg cataagatcg gaaaaaccac
    10501 cgtgattgat tatctgcgcg ccgcgcaaaa cgcgtatttt gtttttgttc tggaaaccgg
    10561 ttcgcccagc gtcaaaaaaa gattgcaatc ggcaaaaaaa atctattcgg ccgacactta
    10621 tttttcggcg cgctacggcg gtgtgttttc gcggaatatc gggcatttga tggagcaggc
    10681 ggtttgtttg gagtttttgc gccgttgcgc ccatgacggg attttggaag tgttcttttg
    10741 gaaagattat cgcgggcgcg aagttgattt tctggcgatg aaaaacggcc aggcaatggg
    10801 actttaccag ataacctatg cggaaaccat ggccgaattg ccggcgcggg aaattgaatc
    10861 gttgctggcc gcggcaaaaa cattttcttg cggcaatttg atggtggtga cttggggctt
    10921 ggaggcggaa attgaaaaag acggccaaaa aataaaattt gtgccgcttt caaaattttt
    10981 atctgacccg tcttttttaa aattttagcc tacttaattt cagccttttt attggatttt
    11041 gttaaagatt aacgattaat gattaaagat tattggttga attcggtcat tgatttttcc
    11101 gggttgtcgg agagggcgag agaaagagcg gatatgaccg tcccgatggc ttgatcaaac
    11161 ggggtgcgat cggcggcggg gatttttttg agcacgtatt tatcggcggg gatttttttt
    11221 gtttgcgcga tcggcgaggc gatgccgatg cgaaagcgga taaagttttt ggttttcagt
    11281 tggtcaatga ttgactgcac gcccttgtgt cccgccgcgc cgcggttttg cgagattttg
    11341 attttaccaa gagggatgtc caaatcgtcg tttatcaccc aaagatcggg gcggaatttt
    11401 ttcgggcgga tttttagata attgaacaat tttgccagcg ctttgccgga attgttcatc
    11461 agcatctgcg gctttgccaa aatcacggtt ttgccgaaga tttctttgcg cgcgaccaaa
    11521 atgccgtcgg gcatcatcgc aaattccgga aattcaaacc gccgcgcaaa ttcgtcaacc
    11581 gcaagaaatc ccgcgttgtg cggcgtgttc tggtatttat ctcccgggtt tcccaaaccg
    11641 ataatgaaaa cggccggcga tttttccatc gttttctttt ccataattag gccattatag
    11701 catattttcc cgccgggtaa tcgcaa
//
LOCUS       DAOQWE010000009        11694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_5961_length_11694_cov_6.836842, whole genome shotgun sequence.
ACCESSION   DAOQWE010000009 DAOQWE010000000
VERSION     DAOQWE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11694
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5961_length_11694_cov_6.836842"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            585..983
                     /locus_tag="PKK37_00795"
     CDS             585..983
                     /locus_tag="PKK37_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80953.1"
                     /translation="MDGLFELYFKTKASFAALFLLTVIYLVVWAAWLWPTFPYPGVSW
                     HGDPWVNFRQWFLLSLLYAIFFVTFSAFRNKWLRDAKKEEARRYGGTTVAQEEKRKPV
                     IGIDEAMKRVAREAAEEVAEEKEKKEGGEK"
     gene            983..2290
                     /locus_tag="PKK37_00800"
     CDS             983..2290
                     /locus_tag="PKK37_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80954.1"
                     /translation="MVIPVEEIFGSISFPWWAMALLTVFVFIPGALGALLYTIYWMVW
                     HYLPSVWRVVIRPKDGRVYQIVSGKEKDKGSVEDENEGRHRYWLLCYAGWKFGNTGDI
                     VLGDDENAGGLLNKGIYMIGVFEAVTQIHQRWAEVKGGRYIPRDVISRGLLVKKAPMA
                     YVVDVFDEDNFPFRFLGNVIMRMCNPDKAFRFTHEAREIVIGFIGSAWLAWAKSRKIY
                     RPSVDISKTDQCIDINVKVNPSEEDSDQIIEEFWSDLTKRTYKYRPFVVRPAAARKDP
                     WPIAWLTSRLPYYKGIYFDYEPGEEKKGSFLEMVWEVYGFEIERMTLDNVILSKDFAE
                     ALSAAIRAKLLGAARLIEAYFEKEAKLQSQEPAERMGAIFDKSPGAAYWELVSALAKN
                     MPASLLDLSAIGTLVNSAVPPGTPDAAKESLIKDIVRVRQTIK"
     gene            complement(2371..3693)
                     /locus_tag="PKK37_00805"
     CDS             complement(2371..3693)
                     /locus_tag="PKK37_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80955.1"
                     /translation="MIKMGESKKLILVTTFFLVALLILVSNETSAFMVPEVMVSNFVK
                     PITTYLFDIIKQLLTGLISFLVAAGLFSFSVNNSPKMLDLKNSDFVITGLSITTSLAD
                     ILLIAVFIAIALGTIFDAEAINAKKHFLKFAAAALLIHFAPLFVGMAIDIGNIIMSGL
                     VVGSGQAAFSSIMNNFAMQIAINLAGIAVAYGGMAVASAIPGGNIALGVALAFGILPA
                     FAFLPGVLIQIMVMGILTGLLFSFAIFFLTRVFMIQILAVLAPLAILASVLPKTESYF
                     KTWLSWLIGWTVGGVLMLFLLTLGLTTAGGFLPNDIDFNSEGFTVGGVLFGLLTMDHI
                     KWIALAVYMITVQTLCAGAIPAISGQISDKISQGAGVAAGKLGQGFRTGKGKEKDPVT
                     GKKPDANFQSAIESKISKRAESYFGTRSSKASSPPGTPPPPAGAAAPP"
     gene            complement(3690..4805)
                     /locus_tag="PKK37_00810"
     CDS             complement(3690..4805)
                     /locus_tag="PKK37_00810"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80956.1"
                     /translation="MLPVLSLFAFGECQEPVSLIDETQVDWPAAPLGGYTLDENSSLA
                     DLVAYFFGWGVGLGGLAVFIALIIAGVQLITSVADPGKMNEAKKRIKSAALGLGLLLS
                     SWAIFNLINPNLTKMEVNLYETKILKAEGKECTSAAECCDVAKNPQCIPKNWRCCQYN
                     DTKCMETGVETPENARGNFAIGAKCLGDNACESLYCGCNYGINPKSGAKDSWGNVCLP
                     NPKICIENPAAPELGCNKVVFYDQIDFGGNEKTIEEGKINAGWVWFDGFRPKSYQAFY
                     KEKDSAGKETGKFLPCGKFACGCQINRCLDAGGAGGSNCINNTANEESGEFMEHAYSE
                     SNFEEISGVRIKDSTKPAGAEEGVPWWQTLSNLNPFK"
     gene            complement(4926..5663)
                     /locus_tag="PKK37_00815"
     CDS             complement(4926..5663)
                     /locus_tag="PKK37_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80957.1"
                     /translation="MKLIHLYLIGVFVMIAFLVGSTMWQFGQFQSNIKDLQFGLPQEL
                     SQGMPDANQQIAQMMSQIQTQAQNSGAATPAGENASTTVETKTWTAPDESFSFEYAAD
                     WTPMAITAPVDSAGGKILFSAYKTGGAAAIPSVNSLSVEESDYKTAEELMEKIESDLR
                     AKNIPAKITRSEIINGAQTIPVIETEYSTTGAILNTSIKFKMVSAAFDNASGKIYLVE
                     SILQNPDATAEQEIETIIKSISGAPKN"
     gene            complement(5694..6995)
                     /locus_tag="PKK37_00820"
     CDS             complement(5694..6995)
                     /locus_tag="PKK37_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80958.1"
                     /translation="MIKTALKKFCAGKRQTAAFLALLAIGLAFIPAISGAQLSEMITA
                     SNLSPYVASTSQYFLTLATQMVQGLSLLMMSSFLLDTAIANSILWIDIQNRFAQTGLN
                     VTLALADALLIAVFVAIAMAYVFKIESFRTEKAILKLFAVALLIHFAPMFVGMITDVA
                     NIMMSGIAANSKSAFSDVFVNDFGVSVGLSILPMIANFNNAASSSLGGVIGIAANLAS
                     LTLTTSNLVAAAPDYIAKIIALNIIAGLIFVHAIFFLARIFIIQALTVLSPLAILCAA
                     MPQTKAYFGMWKSWLLGWTFGGILLLFLLILGINVLNETLALAEPPTASLTMGDKTFS
                     LDAAWIFKWMTLAIYIVSMDMICLSLISTFGARVKSGDGAIKKITRQITTIAGRGEQE
                     VKANTENLPRPATTPRRPPDYRMPERPAAVDIDYPTGTMHG"
     gene            complement(6992..8134)
                     /locus_tag="PKK37_00825"
     CDS             complement(6992..8134)
                     /locus_tag="PKK37_00825"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNR80959.1"
                     /translation="MDKKTQTIIFGAIAAVAMSGAAAGFAAENCQMSPIATKPPQTDW
                     PASPLTSQNLGEETGIAQMIQYFFEWGVGLGGLAVFIALIIAGLQYITSIADPGKVKE
                     AKGRIKSSFIGLAMILSSWAFFNLINPSLNTLQEISMVKSETAGYGAATNLSCEDPWD
                     CCRESGDQCSPENFVCCEKNNFECMQGKGDMKRGSSEIGAACENDWDCASGYCKCHKD
                     EDPWAAKCGENPKVCVKIMGNPEIGCDFVGFFDGPDFTGEETFFKVQYADTGWFTPQK
                     ENEEFMESIRSYQAYKADRNPSNPQQYFMEDGGTTTIIDLARKVPCGQAACGCKLSVC
                     NDPAATQSSCVNSVDYGLAFNMNLDESDREVVRVQDQTKGNVNELR"
     gene            complement(8145..10706)
                     /locus_tag="PKK37_00830"
     CDS             complement(8145..10706)
                     /locus_tag="PKK37_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80960.1"
                     /translation="MIYNQAMRKKSFAAKNLLAVGLFVLSPAAVFAAEISQTEYFNIG
                     SGCGITVEDASAGSITCQPWEKGSQVKVESIDVGTCICANECLAVPKNHYYYNDPINK
                     TRQTNEQEITLPVVLAWDNVDGWTNEEGGYLWQLGGLGGERSVNSKLFGARSYLLEIE
                     NKNLELNDSKSAGSIFRQVLKENEFNPTEKFYPCFFNSDTTIKWRVRPCCNEDGSHCL
                     PEEQAPWWTFKTSPAPEPINIGDADWNGPNGKTGISYNGLKLEWCKAKVSPEKQAYND
                     NMGVALSYQLRVYTNENELVFGSAAVPAQFSQLAAWLKKDVPALQNSLACHYLQKQSD
                     NTCKPDAIYPLTANVARNWWSHKELPAQDRALFTKNITYSWQLRRCFTNSQSGDEYCG
                     SLSNKYWGQIWKFTVKDEPIAAPSPLAPANDNQYADSQSAKLVELPGKLLWQAANGAN
                     SFAYDIQKISGGETSLVGGERRAAKSQILFSKSAAEDANSDAQGIELELDTPYKWRVK
                     SCWPSIPIGNVCNEPWSPWWYFRTTGRAPKTETLRPENEAREVAMPVNLQWEAVPGAK
                     SYILNFNNQEIKVQNNAYTVDYPTINQSRTYKWSVKTCADSPGQTCGQSSPEFSFATA
                     ALGASQNPISPDQTIDNRPLIYNFSWEPVAGAHYYKFTLDYTQRSDKETNEKCQTGGK
                     VEKTVQENSAAVNKNIDALYCLGKYEWKVLACLDEECADAGAAPAVWSFDFVAGGKEI
                     KKGETILAVCGLSNDNPNTEWDDREACGIKHFFLGAMQILNFFLFRLSLILLPLLVLA
                     TGLLFYTSFGTPEIKQKVISWWKVIGIGYGLLFFAWTITGIVVSLTGYTGVWWQI"
     gene            complement(10703..>11694)
                     /locus_tag="PKK37_00835"
     CDS             complement(10703..>11694)
                     /locus_tag="PKK37_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80961.1"
                     /translation="NICSGQWSDYWSFRTTGRKPTIGSDAVKMAAPTATLTWEAISGA
                     NSYRVKVTGDGAPAEEVLTTTNQFGFDYPGAKKNYSWKVKTCADSAGTICGGWQAGTS
                     FNSEDFPVPANLSPAGNVAQLPSALSWNGTAGYFWVTAQVANGEKSTSCDPEWFNKEY
                     EGKTVSGTDLALKAAAKNSSSYCSGDYSFTVRPCFDDKCAARSFNEARQTFSVAGKSE
                     SSGLMVCGQKSDNPGTPYNEKEPCEIKHIFLTLKVIIDFVMFKLALLLLPIMALVTGG
                     LFYLAADKANLAARAKDIWKKIGIGYAILFFSWIIVSIIMALTGYGALWNQIL"
BASE COUNT         2775 a         2834 c         3049 g         3036 t
ORIGIN      
        1 tcgttcaata tccgccgcaa tccctccacg ccgaattttt tgatttgcgc gggcagagaa
       61 atggaatccg cctcgcttaa aatgcggggc caaactatac ttatctgccc gccagtccgc
      121 cccggcgcgg tttattcagc ggaataattt tttagaagcc attttcaaag aggaatgtat
      181 tggatttgcc tgccttcata tttctttgtt cataaaatta tgcgaggtta ttaatagtgt
      241 gcaatgccat tatgcgctat gtatcaaaag tgtttaatgc cattgcacag tttggcctta
      301 attgatttga atatgcttaa aattattttg tatttattcc tcctttggaa aaggaggtgg
      361 cgcgtaatcg cgccggagga tttttaaatt caaaatttca aatccctccc cctaacgggt
      421 actccctttt ccaaagggag aataaatgcc cccagtgagt ggttactctg cgaaaccttg
      481 acaaattgcc caaaatatga tatggagtag ccagtcccgc cgggtctccg gcgctaaaaa
      541 ttattaatat ggatactgga aaatagacaa aaggaggtta aagaatggat ggtttatttg
      601 agctatattt taaaacaaaa gccagttttg cagcattgtt tctcttgacc gtaatttatc
      661 tagtggtctg ggctgcttgg ctgtggccaa cattccccta tcccggtgtt agctggcacg
      721 gggatccgtg ggtgaatttt cgtcaatggt ttcttctgtc tcttctatac gcaatttttt
      781 ttgtgacttt ttctgcattt cgcaacaaat ggttgcggga tgcaaaaaag gaagaggcaa
      841 gaagatatgg tggcactacc gtggcacagg aggaaaagag gaagcccgtc atcggtattg
      901 acgaggcgat gaaaagggta gccagggagg ctgccgagga agtagcggaa gaaaaagaaa
      961 agaaagaagg aggtgaaaag tgatggtaat cccagtggag gagatttttg gtagtatctc
     1021 ttttccttgg tgggcaatgg ccttattgac ggtttttgtt tttataccgg gagcgctagg
     1081 cgcccttttg tataccattt actggatggt ctggcattat cttccctcgg tgtggagggt
     1141 cgttatccgt ccaaaggacg ggcgcgtgta tcagatcgtc tccgggaaag agaaagataa
     1201 aggttccgtg gaagatgaaa atgaagggag gcaccggtac tggctcctct gctacgccgg
     1261 ttggaaattc ggcaacactg gcgacatcgt cttgggagat gacgaaaacg ccggtggatt
     1321 gttaaacaag ggcatttaca tgattggggt ttttgaagcc gtgacccaaa tccaccaaag
     1381 gtgggcggaa gtcaaaggcg gccgatatat tccccgtgac gtcattagcc gcggccttct
     1441 cgtgaagaag gcacctatgg cttatgtagt ggatgtcttt gatgaggata actttccatt
     1501 cagatttctt ggaaacgtta tcatgagaat gtgcaatccc gataaagcgt tccgctttac
     1561 ccatgaagct agggagatag ttattgggtt catcgggagc gcatggttgg catgggcgaa
     1621 gagtcgaaag atttatcgtc catcggttga tattagcaag accgatcagt gcattgacat
     1681 taatgtcaag gttaacccca gcgaagagga ctccgaccaa ataatagagg agttctggag
     1741 cgacctcacg aagagaacct ataagtatcg gccgtttgtt gtaagaccag cggcggcgcg
     1801 aaaagatcct tggccaatag cttggctcac ttcccggctt ccatactaca aaggcatcta
     1861 ctttgattat gagccggggg aagaaaaaaa aggctctttt ctggaaatgg tatgggaggt
     1921 atacggtttt gaaattgaaa gaatgacatt agataatgtc attctgagca aagattttgc
     1981 cgaagcgctt tccgcggcaa taagagctaa actactaggt gccgcgcggt taattgaggc
     2041 ctactttgag aaagaagcca agcttcagag tcaggagccg gcagaaagaa tgggggcaat
     2101 ttttgataaa agccctggag cggcctactg ggagttggtg agcgcgctgg ccaaaaacat
     2161 gccagcgagc ttattggatc tcagcgctat tgggacattg gttaacagcg ctgttcctcc
     2221 cggtacccct gacgcggcaa aagaaagcct gatcaaggac atagtccggg tcaggcagac
     2281 gataaaatga agccgatagg attgctttca cccgagcgat cctctttttt tagaatttcg
     2341 gtggacgttt tgatgggtta tgattatttg ctatggcggc gcggctgctc ctgccggagg
     2401 cggcggcgtt cccggaggcg acgatgcctt tgaagaccgc gttccgaaat aagattcggc
     2461 gcgtttgctt atcttggact caatcgctga ttgaaaattg gcgtctggtt tttttccggt
     2521 aaccgggtct ttttctttgc ctttgcccgt cctaaatcct tggcccaatt ttccggcggc
     2581 gacgccggct ccttggctga tcttgtcgct gatttggccg ctgattgccg gaattgctcc
     2641 ggcgcaaagg gtttgcacgg tgatcatata gaccgccaag gcgatccatt tgatgtgatc
     2701 catcgtgaga aggccaaaaa ggactccgcc gacggtaaat ccttccgaat taaaatcaat
     2761 gtcgttgggt aaaaatcctc cggcggtggt gagtcccaag gtcagcaaaa acagcattaa
     2821 aacgccgccc acggtccagc cgatgagcca gctgagccaa gttttaaaat aggactcggt
     2881 tttgggcaag acgctggcca aaattgccaa cggagcgagc acggccaaaa tttggatcat
     2941 aaatacccgg gtcaggaaaa aaatcgcgaa cgaaaaaagc agaccggtca aaatccccat
     3001 caccattatt tgtattaaaa cgccgggcaa aaacgcaaag gccggcagaa ttccaaaagc
     3061 taacgctacc ccgagggcaa tgttgccgcc gggaatggcg gaagcaactg ccatcccgcc
     3121 ataagcaacg gcaatccccg caagattaat ggcaatttgc atcgcgaaat tgttcataat
     3181 ggaagaaaac gccgcttggc cgctgccaac gacaagcccg gacataatga tatttccgat
     3241 gtcaattgcc atcccgacga acagcggagc aaaatggatt aacagtgccg ccgcggcaaa
     3301 tttcagaaaa tgttttttgg cattaattgc ttcggcgtca aaaattgttc ccagagcgat
     3361 ggcgatgaac actgcaatca gcaaaatgtc ggccagcgac gtggtgatac ttaagccggt
     3421 aatgacaaaa tcgctgtttt tcaggtccag cattttggga ctattattga ccgaaaagct
     3481 gaacaagccg gcggcaacca aaaaagaaat taagccggta agcaactgct tgataatgtc
     3541 aaaaagatag gtagttatcg gcttgacgaa gttggagacc atcacttccg ggaccataaa
     3601 tgccgaagtt tcgtttgaaa ccaagattaa cagagctacc agaaaaaatg ttgtgactag
     3661 tattagtttt ttgctttccc ccattttaat catttgaatg gatttagatt tgacaaagtt
     3721 tgccaccatg ggacgccttc ctccgcgcct gccggctttg ttgagtcctt gattctcacg
     3781 cccgaaattt cttcaaaatt agattcactg tatgcgtgtt ccataaactc cccgctttct
     3841 tcgttggcgg tgttgttgat gcaattggag ccgccggcgc caccggcgtc caaacagcgg
     3901 ttgatctgac agccacaggc gaattttccg cacggcagaa atttaccggt ttctttgccg
     3961 gcgctgtctt tttctttata gaacgcttga taagattttg gcctgaatcc gtcaaaccaa
     4021 acccatccgg cgttgatttt tccttcctct atcgtttttt cattgccgcc aaaatcgatt
     4081 tggtcgtaaa aaacaacctt attgcaacct aattccggcg ccgccggatt ttcaatgcaa
     4141 attttgggat tgggcaaaca gacgtttccc cagctgtctt tggcgccgct tttcggattg
     4201 atgccgtaat tacagccgca ataaagagat tcgcaggcat tgtcgcccaa acatttcgcg
     4261 ccaatcgcaa aattgccgcg cgcgttttcc ggtgtttcta cgccagtttc catgcacttg
     4321 gtgtcattat actggcaaca gcgccaattt ttggggatgc attgcgggtt ttttgcgaca
     4381 tcgcaacatt ccgccgcgct ggtgcattct tttccttcgg ccttgaggat ttttgtttca
     4441 tagaggttga cttccatttt ggttaaattg ggattgatca aattaaaaat cgcccagctg
     4501 gacaacagca agcccagccc cagcgccgcg gattttattc tttttttggc ctcgttcatt
     4561 ttgccggggt cggcgacgga agtgatcagt tgcacgccgg cgatgattaa cgcgataaaa
     4621 accgccaaac cgcccagtcc cactccccag ccgaaaaaat aggcgaccag atccgccaag
     4681 ctggaatttt catccagcgt gtatcctccc agaggagcgg ccggccaatc cacttgtgtt
     4741 tcatcaatca atgaaacggg ttcttggcat tctccgaaag cgaacaggga cagtaccgga
     4801 agcatcaaaa aaagaaaatt gattccgacg aaaaacagaa aatatttccc gctttttggc
     4861 ccaaaaatgc cgcggatttc attttgaaac ggtttgtttt ttgatggttt tcggttcatt
     4921 tgagtttaat tttttggtgc accagaaatt gatttaatta tcgtttcaat ttcctgctcc
     4981 gccgttgcgt ccgggttttg caggattgat tcaacgaggt aaattttccc ggaggcgtta
     5041 tcgaaggcgg cactgaccat tttaaacttt attgaggtgt tcaagatcgc ccccgtggtt
     5101 gagtattcgg tttcaatcac ggggatggtt tgggcgccgt tgatgatttc actgcgggtg
     5161 attttggcgg ggatgttttt ggcgcgcaag tcgctttcaa tcttttccat cagttcttcg
     5221 gcggttttgt aatcggattc ttccacggaa agggagttga cggaaggtat tgccgccgca
     5281 ccgccggttt tgtaggcgga aaacaggatt ttgccgccgg cgctgtccac gggggcggta
     5341 atcgccatcg gcgtccagtc ggccgcatat tcaaaggaaa acgattcgtc gggggcggtc
     5401 caggttttgg tttcaacggt ggtgctggcg ttttcgcccg cgggcgttgc cgcgccggaa
     5461 ttttgcgcct gtgtttggat ttgactcatc atctgggcga tctgttgatt ggcgtcgggc
     5521 attccctggc tcaattcctg cggcaatccg aattgcaggt ctttgatgtt ggactgaaac
     5581 tggccgaact gccacattgt ggagccgacc aaaaaagcga tcatcacgaa cacgccgatt
     5641 aaatatagat ggatcaattt catagcggaa tgtaagaaat taaaatttaa aaattaaccg
     5701 tgcattgtgc cggtgggata atcaatatca accgccgccg gcctttcggg cattcggtaa
     5761 tccggcggcc gccggggcgt cgttgccggt cggggcaaat tttcggtgtt ggctttgacc
     5821 tcttgctctc cccggccggc gatggtggtg atctgccggg tgattttttt aatcgcgccg
     5881 tcgccggatt ttacccgggc gccgaaagtt gaaatcagcg acaggcaaat catatccatg
     5941 gagacgatat agattgccag cgtcatccac ttgaaaatcc acgccgcgtc caaagaaaaa
     6001 gttttgtcgc ccatggtgag ggacgcggtc gggggctccg ccagggcgag ggtttcgttg
     6061 aggacgttga ttcccaaaat caacaggaac agcaataaaa tgccgccgaa cgtccacccc
     6121 aaaagccacg atttccacat tccgaagtaa gcttttgtct gcggcatggc ggcgcaaagt
     6181 atcgccagcg gcgacagcac cgtgagtgcc tgaatgataa aaatccgggc gagaaaaaat
     6241 atcgcgtgga caaatatcag tccggcaata atgtttaacg cgatgatttt ggcgatataa
     6301 tcgggggcgg cggccaccag attgcttgtt gtgagcgtca acgatgctaa attggcggcg
     6361 atgccgatca ctccgcccaa acttgatgat gccgcgttgt tgaaattggc gatcatcggc
     6421 aggatgctca atccgacgct tacgccgaaa tcattgacga agacgtcgct gaaggcggat
     6481 ttgctgttgg cggcgatgcc gctcatcata atgttggcga catcggtgat catgcccacg
     6541 aacattggcg cgaagtggat caatagcgcc acggcaaaaa gtttcaagat tgctttttcc
     6601 gtcctgaacg attctatttt gaaaacatac gccatggcga tggcgacaaa caccgcaatc
     6661 aataacgcat cggccaaagc gagggtgacg ttgagcccgg tttgcgcaaa ccggttctgg
     6721 atgtcaatcc acaaaatgga attggcgatg gccgtgtcca gcaagaacga cgacatcatc
     6781 agcagggaca atccctgtac catttgcgtg gcgagggtca aaaaatattg agaggtggag
     6841 gcgacatagg gagacaaatt ggaagcggtg atcatttccg aaagctgggc gcccgaaatc
     6901 gcggggatga atgccaatcc gatcgccagc aacgccaaaa acgccgcggt ttgccgcttg
     6961 ccggcgcaaa atttttttaa tgccgttttt atcatcgcaa ttcgttaacg tttccctttg
     7021 tttggtcctg cacgcgcacc acttcgcgat cgctttcgtc cagattcatg ttaaacgcca
     7081 gtccgtagtc aacgctgttg acgcaggagc tttgggtcgc cgcgggatca ttgcaaacgc
     7141 ttaatttgca accgcaagcc gcctggccgc acggcacttt ccgtgccaga tcaatgatcg
     7201 tggtggtgcc gccgtcttcc ataaaatatt gctgggggtt actgggattc cggtcggctt
     7261 tgtatgcctg atacgaccgg atgctttcca taaattcttc attttctttt tgcggcgtga
     7321 accagccggt gtccgcatat tgaaccttga aaaaagtttc ttcgccggtg aaatccggtc
     7381 cgtcaaaaaa gccgacaaaa tcgcaaccga tttccggatt tcccataatc ttgacgcaga
     7441 ccttcggatt ctcgccgcat ttggccgccc aaggatcttc atctttatgg cacttgcagt
     7501 atccggaggc gcaatcccaa tcattttcgc aggcggcgcc gatttcggaa cttccccgtt
     7561 tcatatctcc ttttccttgc atacattcaa aattgttttt ttcacagcag acgaaatttt
     7621 ccggagaaca ttggtcgccg ctttcccggc agcaatccca gggatcctcg caggaaaggt
     7681 tggtggcggc gccgtatccg gccgtttcgc tttttaccat ggatatttcc tgcaaagtgt
     7741 ttaaagacgg gttgatcaga ttgaaaaacg cccagctgga gagaatcatc gccagtccga
     7801 tgaacgacga ttttatccgc cctttggcct ccttaacttt cccgggatcg gcgattgacg
     7861 tgatgtattg caggccggcg atgatcagcg cgataaaaac cgccaatcct cccaatccca
     7921 ctccccattc aaaaaaatac tggatcatct gcgcgatgcc ggtttcctcg ccgagatttt
     7981 gggaagtgag cggcgaagcg ggccaatcgg tttgcggcgg ttttgtggca atcgggctca
     8041 tttggcagtt ttccgcggcg aatcccgccg cggcgccgga catcgctact gccgcgatgg
     8101 ctccaaaaat tatcgtttgc gtttttttgt ccatagattt taccctatat ttgccaccaa
     8161 acgccggtat atccggtcaa agaaacgaca atgccggtga tggtccaggc gaaaaacagc
     8221 aggccgtaac cgatgccgat cactttccac caggagatca ccttttgctt gatttccggc
     8281 gtgccgaaac tggtgtagaa aagaagtccg gtggccaaaa ccagcagggg caagaggatc
     8341 aacgacaggc ggaaaaggaa aaaattgaga atttgcattg cgcccaagaa gaaatgcttg
     8401 atgccgcagg cttcgcggtc gtcccattcg gtgttggggt tatcgttgga caggccgcaa
     8461 accgccagaa tcgtttcacc ttttttaatc tctttgccgc cggcgacaaa atcaaacgac
     8521 cagactgccg gcgccgcgcc cgcatcggcg cattcttcgt ccaggcaggc caaaaccttc
     8581 cattcgtatt ttcccagaca gtagagcgcg tcaatgtttt tgttcaccgc cgcggaattt
     8641 tcctgcacgg ttttttcaac cttgccgccg gtttggcact tttcgttggt ttccttgtcg
     8701 gatctttggg tatagtcaag cgtgaatttg taataatgcg cgccggcgac cggctcccag
     8761 gaaaagttgt aaatcagcgg gcggttgtca atggtttggt cgggagatat cggattttgg
     8821 gacgcgccca gcgcggcggt ggcaaaagaa aattccggcg aactttggcc gcaagtctgg
     8881 ccggggctgt cggcgcaggt tttcacgctc catttgtaag tccggctttg gttgattgtc
     8941 ggataatcaa cggtgtaggc gttgttttga actttgattt cttgattgtt aaaattcaaa
     9001 atgtatgatt tggctcccgg caccgcctcc cactgtaaat taaccggcat tgccacttcc
     9061 cgcgcttcgt tttccgggcg caaggtttcg gttttggggg cgcggccggt ggtgcgaaaa
     9121 taccaccacg gcgaccaagg ctcgttgcaa acgtttccta tcgggatgga cggccagcag
     9181 gatttcaccc gccacttgta gggcgtgtcc aattccagct caattccctg ggcgtcgctg
     9241 tttgcgtctt ccgccgcgga tttggagaag agtatttgtg attttgccgc gcgccgctct
     9301 cctccgacca gcgaggtttc gccgccggaa attttttgga tatcataggc gaatgaattg
     9361 gcgccgttgg cggcttgcca caaaagtttc ccgggaagtt caaccagttt ggcgctttgg
     9421 gaatcggcgt attggttgtc gttggccggc gccagcggcg agggtgcggc aatcggttcg
     9481 tctttgacgg tgaacttcca gatctgcccc caatatttgt ttgaaagcga gccgcaatat
     9541 tcgtcgccgg actgggaatt ggtgaagcac cggcgcagtt gccagctgta agtgatgttt
     9601 ttggtgaaca gcgcgcgatc ctgcgcgggc aattctttgt gagaccacca attgcgggcg
     9661 acattggccg tgagcggata aatcgcatcc ggtttgcaag tgttgtcgct ttgtttttgc
     9721 aggtaatggc aggccaaaga attttgcagg gcggggacgt cttttttcag ccatgcggct
     9781 aattgggaaa actgcgccgg caccgccgcg ctaccgaaca ccagctcgtt ttcgttggta
     9841 taaacccgca gttggtatga aagcgccaca cccatattgt cgttgtaagc ctgtttttcc
     9901 ggagacacct tggctttgca ccattccagc ttcagtccgt tgtaagagat gccggtcttt
     9961 ccgttcggac cgttccaatc ggcatcgccg atattgatcg gttcgggcgc ggggctggtt
    10021 ttgaatgtcc accagggggc ttgttcttcg gggaggcagt gcgatccgtc ttcgttgcaa
    10081 cacggccgaa cgcgccattt gatcgtcgtg tcggaattga aaaaacaagg ataaaacttt
    10141 tccgtcgggt tgaattcgtt ttctttgaga acctggcgga atatactgcc ggcgcttttt
    10201 gaatcgttta attcaagatt tttattttca atttccaaaa gataggagcg cgcgccgaac
    10261 aattttgaat taacggaccg ctcccccccc agtcctccca actgccacaa atatcctcct
    10321 tcttcgtttg tccatccgtc aacattgtcc cacgccaaca ctacgggcag ggtgatttcc
    10381 tgttcgttgg tctggcgggt tttgttgatc gggtcgttgt agtaataatg atttttgggc
    10441 acggccaaac attcattggc gcaaatgcag gtgccgacat caatgctttc cactttaact
    10501 tgggatcctt tttcccatgg ctggcaagtg atggaaccgg cggaggcgtc ttccacggtg
    10561 atgccgcacc ccgaaccgat attgaaatat tcggtttggc tgatctccgc ggcgaaaacg
    10621 gccgccggcg ataaaacaaa caacccgacc gccagcaaat ttttagcggc aaaagatttt
    10681 ttcctcattg cttgattata aatcataaaa tttggttcca caacgccccg taaccggtaa
    10741 gcgccattat tatggaaaca ataatccacg aaaaaaacag aatggcgtag ccgatgccga
    10801 tttttttcca aatatctttg gcgcgcgccg ccagattcgc tttatccgcc gccagataga
    10861 acaggccgcc ggtaacgagc gccattatcg gcagaagcag gagtgccagc ttgaacatca
    10921 caaaatcaat gataactttc agggttaaaa atatgtgctt gatttcgcag ggttcctttt
    10981 cgttataagg agtgccgggg ttgtcgcttt tttggccgca aaccatcagc ccggaacttt
    11041 cgctcttgcc ggcgacggaa aatgtttgcc gcgcttcgtt aaaactgcgc gccgcgcatt
    11101 tgtcgtcaaa gcagggtctc acggtgaacg agtaatcgcc ggaacaatag gaagaggaat
    11161 tttttgccgc ggccttgagc gccaaatcgg tgccggagac tgtttttccc tcatattctt
    11221 tgttgaacca ttccggatcg caactggtgc ttttttcgcc gttggccact tgcgccgtga
    11281 cccagaaata tccggcggtg ccgttccacg acaaagcgga gggcaattgc gcgacattac
    11341 cggccggcga caaattggcc gggacgggaa aatcttcgga attaaacgat gttcccgcct
    11401 gccatccgcc gcaaatcgtg ccggcgctgt cggcgcaagt tttaactttc caggaataat
    11461 tttttttggc tccgggatag tcaaagccaa attgatttgt ggtggtaagg acttcttccg
    11521 ccggggcgcc gtctccggta actttaacgc gatacgaatt tgcgcccgaa atcgcctccc
    11581 aagtcaaagt ggcggttggc gcggccattt tcaccgcatc gcttccgatg gtcggttttc
    11641 ttccggtggt gcgaaaagac caataatcgg accattggcc gctgcaaatg tttt
//
LOCUS       DAOQWE010000010        11447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_6108_length_11447_cov_7.305126, whole genome shotgun sequence.
ACCESSION   DAOQWE010000010 DAOQWE010000000
VERSION     DAOQWE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6108_length_11447_cov_7.305126"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..988
                     /locus_tag="PKK37_00840"
     CDS             332..988
                     /locus_tag="PKK37_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80962.1"
                     /translation="MSGIEYFSIIIVKNEKFMPEEKIFNAIRHIIESLEIVGGLMLLS
                     MEPGRISSRARQKRIDDFLEEKMFGKDKLADNRDDNRVKIQNKFYYLKKRGLVNVEYR
                     GNQIYISLTDEGKKRAGKYKIDDLKIKTAENWDKKWRVLIFDVEENYRIKREALRGKI
                     KELGLYQLQKSVWVCPYEFKKEMSILRDFFGLEKGDMKVITAVEIEDDQPVRDFFGLK
                     "
     gene            1198..1944
                     /locus_tag="PKK37_00845"
     CDS             1198..1944
                     /locus_tag="PKK37_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNR80963.1"
                     /translation="MSYKDYIIFALAVLALILLILMSPAGKDFWQSLPKFSDLATSPA
                     TRELAERKPPENSVPAADSTPEITAEIPANEEYQEPAFEENISETKVAIVPVQPSTAP
                     DAPMADIIMIPEINITAPLVTAPAGTDAAKLKKMLDSGAVVYPDSAPFGGVGQTIVLG
                     HSAPPGWPEIKHDTIFSRIAELGFGSKIVAVYNDKTYTYSTVENQIIEKGGDIPKLAA
                     ADNALVLVTCWPPGRDLKRMVVQANLVSVE"
     gene            2014..2685
                     /locus_tag="PKK37_00850"
     CDS             2014..2685
                     /locus_tag="PKK37_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80964.1"
                     /translation="MQNKIIAAGLGIAIVIAAGWYVLSRREAAPAGNGNEQNQGQEAG
                     KSEENENGDNFAGTLGDLMARKTPVKCEAGLDIEGKPQKQTMYFAGDKFRVEMAMQID
                     EYTNNTFMIVKDGWQYVWSQGDMPGLLQAGVKMKFDAETGGDGQSDAPMQNGGVDMDQ
                     VMNFSCVPWVPDASKFELPAGIEFTDLSATMQNLETSPQDLCAVCEMIPDAAAKAECE
                     AGCAQ"
     gene            2737..>2951
                     /locus_tag="PKK37_00855"
     CDS             2737..>2951
                     /locus_tag="PKK37_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80965.1"
                     /translation="MAGSNNQNNNSVDYRELVEFFGEQFDKMNAKIDIIIETKADKTN
                     TATKADIDRVLTRIAMINTKIDDYRAE"
     assembly_gap    2952..3051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3454..6522
                     /locus_tag="PKK37_00860"
     rRNA            3454..6522
                     /locus_tag="PKK37_00860"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02540"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02540"
     gene            6610..6716
                     /gene="rrf"
                     /locus_tag="PKK37_00865"
     rRNA            6610..6716
                     /gene="rrf"
                     /locus_tag="PKK37_00865"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            6821..7045
                     /locus_tag="PKK37_00870"
     CDS             6821..7045
                     /locus_tag="PKK37_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNR80966.1"
                     /translation="MANNLDIVSMSLPKQLDEQIEKEARTGKFASKSEFVRFLVRLWQ
                     EDKLAKEIDEAEKDIAAGRVKEIKSLKELR"
     gene            7057..7314
                     /locus_tag="PKK37_00875"
     CDS             7057..7314
                     /locus_tag="PKK37_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HNR80967.1"
                     /translation="MKIIPTAKFERNYKKLSGSLKDKAEIALEIFKIDLSDSRLRVHK
                     LHGRFAEYWAFTVSYDCRIIFRYGENNEIFLITIGDHKIYD"
     gene            complement(7653..7808)
                     /locus_tag="PKK37_00880"
     CDS             complement(7653..7808)
                     /locus_tag="PKK37_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80968.1"
                     /translation="MIDDTIKTAIALQDAENYDDDILADDDLEDDDAQMGDDDDDDRD
                     DAGDGEW"
     gene            8062..8907
                     /gene="mreC"
                     /locus_tag="PKK37_00885"
     CDS             8062..8907
                     /gene="mreC"
                     /locus_tag="PKK37_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNR80969.1"
                     /translation="MKANFSKKWKVALAITGVAGVILALNIPAVNAQFKNAAFAALAE
                     IQKNVWAAGASAGESLPVFAGESLAEENRRLRAQASGFWVQQAQIDALKKENEFLRQG
                     LNLELEKEFDLKLANIAGKILARDILIIDKGAHDMIAPGMMAIDSQKAVVGKVIKTYD
                     NFSEIQLITDKDFSFDISIGENAIGGLFRGLGGLNAEIGLVPRDAALAPGQRVYTSGL
                     GGIFPAGLLAGAVKEIRQNDVETFQTAAIAPAFDVAAARQVFVAVGKHPMDSALSAEI
                     NGGND"
     gene            8900..9217
                     /locus_tag="PKK37_00890"
     CDS             8900..9217
                     /locus_tag="PKK37_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80970.1"
                     /translation="MIKKLILIAAAVIALFLLQTSFAPHFPFFAGRWFEWANFITLPV
                     VLFALFERRRHNLGWGLAVLGGFLLDIYSARFFGFWMIILLFAVLAIKLGIKKYVRLP
                     SFW"
     gene            9192..11306
                     /gene="mrdA"
                     /locus_tag="PKK37_00895"
     CDS             9192..11306
                     /gene="mrdA"
                     /locus_tag="PKK37_00895"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNR80971.1"
                     /translation="MFGFLRFGKKRRISLSSPEEIEPQEIFCDRRAQRQNKCGDQDCK
                     KFEVAAPAGGLALPAVFAALFFLAAFLRAGYLQIVEGGELMKQAQKNQYIFQKVQSDR
                     GVIYDAGFNVLAKNLSAFDLQCDIQKFSNNPAERKRIIESLAAISGADAAAIDQAVGA
                     QKTAVLENLDHQSLIILEARINDFPGCQVVARPIREYVRDAGLAHLLGYMSKINQDEW
                     SANAENYSINDYIGRSGLEKSYETVLRKDPGKLRVERDANGNVIAQEMTALPESGDSI
                     RLWLDYDLQKKLRDSMKNQLESLGLKKGAAIALDPKTGGVLAMVSFPDYDNNVFSQHN
                     REEIQKLVDDKETNTLLNRAIGGRYLTGSAIKPFIASAALQEGIIAADKNINCQGRIS
                     VQNQYDPEIVYHYNDNHVHGQTNMYKAIAESCNVYFYTIGGGYEDQNGLGPTRIKKYL
                     DLFGWEEPTGIDLPGEIAGFVPSADWKKERFAGTQDQNWTDGNTYWLSIGQEFIGITP
                     IEVASAYAAIANGGTLYRPQMVKAVVDSAKNTIEEKKPEVIRENFIDAAHLAAVREGM
                     RFAVNGAGAPLASALTLNQLGIRMGAKTGTAQLYKGPDGKDLLNSWVNVFAPYDDPQI
                     VLLVMAEEVREGTVAVLPVAKEVLGWYFEPKNEDGVRLSELPPAEKSETSGETGDMLD
                     IEGAMINENPTLPPATIPDEEY"
BASE COUNT         3131 a         2411 c         3078 g         2727 t
ORIGIN      
        1 gccgcccttg acaaattttt cattatatgc ttacattaag gtaggccatc cgaaaggcag
       61 ccctcctatc tattaagtat tccttttttt cggatggcac atgcgaggag gcgagagttt
      121 aaagatttta cccccttcga actactcccc tttaactctt gcccctcgct tcctacttta
      181 atctcaacag cctttcttgt ttttttggat tgatattttt tttgtaagaa acggcattgt
      241 tcggcaatcc acatcgctct gactgaggat tgttttttta taaatctacc cctgcatttc
      301 caaatcctga cattttttgc ggtcgcaaaa tttgtcagga attgaatatt tcagtataat
      361 aattgtcaag aacgaaaaat ttatgcccga ggaaaaaata ttcaacgcaa tcaggcatat
      421 tattgaatcg ctggaaattg tcggcggatt aatgttgttg tcaatggagc cgggcagaat
      481 atcttcaagg gccaggcaga agcggataga cgattttttg gaagaaaaaa tgttcggcaa
      541 agacaagctt gcggacaatc gcgatgacaa ccgagtaaaa atccaaaata aattttatta
      601 tttgaaaaaa agagggctgg ttaatgttga atatcgcggc aatcaaattt atatttcgct
      661 caccgatgag gggaagaagc gggccgggaa atacaaaatt gatgatttga aaattaaaac
      721 tgcggaaaat tgggacaaaa aatggcgggt gctgatattt gacgttgagg aaaattatag
      781 aattaaaaga gaggcattgc gcggcaaaat caaggagctg ggactttatc aattacaaaa
      841 gagcgtttgg gtttgcccct atgagtttaa aaaagaaatg agtattttgc gcgatttttt
      901 cggtcttgaa aagggggata tgaaagtgat taccgccgta gaaattgaag atgaccagcc
      961 ggtccgcgat tttttcggcc tgaaataatt ggtttcggca attgtgccgt tttttgtttg
     1021 gtttgggaat gacttgtttt tggcggtagc cggtctgctt gattttatat cacaacactt
     1081 tttaaattcc tgacaaattt tgcgaccgca aaaaatgtta ggaattcaaa gtttaaaatt
     1141 catattgacc ggcggcggat ttttttgtag gatgggacta atcaattaag aagcaatatg
     1201 tcctacaaag actatataat ttttgcgctg gcggtgttgg cattaatctt gctgattttg
     1261 atgtcgcccg ccggaaaaga tttttggcaa tccttgccca aattcagtga tctggcgacg
     1321 tcgccggcca cgcgggagtt ggcagagaga aagccgccgg aaaattccgt gccggcggcg
     1381 gattccacac cggaaattac tgcggaaata ccggcaaacg aagaatatca agagccggca
     1441 tttgaggaaa atattagcga aacaaaggtg gcgatcgtgc cggtgcaacc gtccaccgca
     1501 ccggacgcgc cgatggccga tataattatg attccggaaa tcaatatcac cgcaccgctg
     1561 gtgacggcgc cggcggggac ggacgccgcc aaactgaaaa aaatgctgga cagcggcgcg
     1621 gtggtttatc ccgattcggc gccgtttggc ggggtgggac aaacgattgt gctgggacac
     1681 agcgcgccgc ccggctggcc cgaaatcaag cacgacacga tattttcccg tatcgcggaa
     1741 ttgggctttg gctcaaaaat cgtggcggtc tacaacgaca aaacctacac ttattcaacg
     1801 gtggaaaacc aaatcattga aaaaggcggc gatattccca aactggcggc cgccgataat
     1861 gctctggtat tggtaacttg ctggccgccc ggccgcgacc tcaaacgaat ggtggtgcaa
     1921 gcgaatctgg tgtcggtgga ataattcgtg gttgttgatt tttgcaagtt tgattttcgt
     1981 gttataaata atgcataaat atcagaaaaa aatatgcaaa ataaaattat cgcggcgggg
     2041 ttggggattg cgatcgtgat cgcggcgggt tggtatgttt taagccggag agaagccgcg
     2101 ccggcaggca atgggaatga gcaaaaccaa gggcaggaag cgggcaaatc cgaggaaaac
     2161 gaaaacggcg ataattttgc cggcactctg ggcgatctga tggcccgaaa aacacccgtt
     2221 aaatgcgaag cggggcttga tattgagggc aaacctcaaa aacaaacgat gtattttgcg
     2281 ggcgataaat ttcgtgttga gatggcgatg cagattgacg aatatacgaa caacactttt
     2341 atgatcgtta aagacggctg gcaatatgtt tggagtcagg gcgatatgcc cggcttgctt
     2401 caagccggtg tgaaaatgaa atttgacgcc gaaaccggcg gtgacggcca gtcggatgcg
     2461 ccgatgcaaa acggcggcgt tgatatggat caagttatga atttttcttg cgtgccgtgg
     2521 gtgccggatg catcaaagtt tgaattgccg gcgggaattg aatttaccga tttgagcgca
     2581 acgatgcaga atttggaaac ctcgccgcaa gatctctgcg cggtctgcga aatgattccg
     2641 gatgccgcgg ccaaagccga atgcgaagcc ggctgtgccc aatagaattt gacaccggag
     2701 attgcgattg gtaaaattga attgcaatca aaaaaaatgg cgggatcaaa caatcaaaat
     2761 aataattccg ttgactaccg cgaactggtg gagttttttg gcgaacaatt tgataagatg
     2821 aacgcgaaga ttgatataat tattgaaact aaagccgata aaaccaatac cgccacaaaa
     2881 gccgatattg atcgggtgct gacgcgtatt gcgatgatca acaccaaaat tgacgattat
     2941 cgcgcggaac annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggttttcca
     3061 caattcggcg gcgcgggcgg ccttgacacg gggttgatca tctgctagga ttgcaatgtc
     3121 agggagataa aacaacaaat gttgacaacg gacaataaca aaaaacatta acgaagcgaa
     3181 tagttttctg cgactttgcg tttcagaaga tcgcttcaat gcggtttttt tgttgcccga
     3241 ttcggattga gcgagggggt ggaacattgt caattgaata gttagttata ttgccggtgg
     3301 atcaaacatc gaatccatcg gtgattccaa agtctttttg aaacaattgt aatttcaaga
     3361 attcggcggc ggcaaagcgg ccatcagcgg tgatggctta attcgcccaa ccgcggagtt
     3421 caacaaaatt gaagttatta attgctagcc aaggtaaatg ttttataggc ggatggtgga
     3481 tgccttggga ccgggaggcg acgaaggacg cggcatgtct gcgataagct tgggggaggc
     3541 gaccaacagc cattgatccc gagatttccg aatggggaaa ccccagccgt gcaaacacgg
     3601 ttgatcccgc gcgcaagcgc gggaagcgaa cctgcggaag ggaaacatct cagtacgcag
     3661 aggaaaagaa aaaaaatcct gagacgcaag tcaaaggaaa cattccccca gtagcggcga
     3721 gcgaaaaggg aagagctcaa acccgtttgt atttctccgg tgttcataaa ttatcattga
     3781 taaattatca ttgatcattg tggttaccgg tgaagtacaa gcgggggtta taagataagg
     3841 atgttcccga gcgtaagcga gggacagagc gcatcaatat gtaatttggt tagcccaact
     3901 accctggaaa gggaggccat agcgggtaac agccccgtaa gcgaaaatca ttacaagctt
     3961 tgaatcttta ttcttgagta ccgcgggaaa cgacagtcct gcgggaagtc ggcgggacta
     4021 taccgccaaa gctaaatact acccggtcac cgatagtgta caagtaccgt gagggaaagg
     4081 tgaaaagcag tccggtgagg acggtgaaat agttcctgaa accatcacgc ttacaaagac
     4141 tcggagcctt gcgttacaca ggggaattcg aatgttcgtg tttcggattt tcgcgtgtag
     4201 cgcggggtga cggggtgctt tttgtagaac gagccaacga gtttaattta tcagcagtct
     4261 aagcgtctct gacgcggagg cacagggaaa ccaagtctta atagggcgaa atatatgctg
     4321 ataggttaag acccgaagcc aaggcgagct tgccatggcc aggttgaatc ccgccgaaac
     4381 gcgggaggag gaccgaaccg gttgactgtg caaaatcttc ggatgagctg tggtaagaag
     4441 tgaaaagcta atcgagcttg gtaatagctg gttctctccg aaataacttt agggttagcg
     4501 ttttgcgtta atgattccgg gggtagagca ctgacagaaa tatcgcgggg aaactcagat
     4561 atttccatca aactccgaat accggaatta ttgcaaagca gtaagactac gggcgctaag
     4621 gtccgtggtc gtgaggggaa aagcccagat cttcgtctaa ggtccttaaa ttaagctaag
     4681 tgcaaaacaa ggaagtgaag ttcctctgat agctaggagg ttagctcaga agcagctatc
     4741 ctttaaaaag tgcgtaacag cttactagct aaggaatttt gcgccgtaaa tacacggggc
     4801 tcaagcttaa taccgaagac aagggtttcc cgcatttatg cggggagcgg taggagagcg
     4861 ttcccaatgc ggcgaagccg aaccgtaagg tttggtggag cgttgggaag tgagaatgtt
     4921 ggcatgagta accgcaatgc gggtgagatc cccgcacacc gaaaacccaa ggtttccgcg
     4981 gcaatggtga tcatccgcgg gttaggcggt cctaaggtca agccgagagg cgcagccgat
     5041 ggacattcgg ttaatattcc gatccttcct ttttttgccg agagagtgac gaagtttagt
     5101 aatccgagcg ggttattgga ttcccgtcgg cagttcgact cccgattccg agcgcaagcg
     5161 agggaaaggg accaggattg acgaaaagtc ccggttcgcc ggggcggatt cggaccagca
     5221 aacttcccgg aaaaacctct caagggttat caaaaaaagg gaaccgtacc gtaaaccgac
     5281 acaggtgggt cggcgtgaaa acgccaaggt gaacggatga aagttcgtga aggaactagg
     5341 caaacaagta tgcgtaactt cggaatatgc attcccccca cttcggtggg gggcgcagcg
     5401 aaagactctc aggcggctgt ttatcaaaaa cacaggtccc tgctaaactc gcaagaggaa
     5461 atataggggc tgagacctga ccagtgcccg tgtgtcaaaa ctggcggtgg tatcgtcgca
     5521 agacggtatc gctaaccggt ctaagctacg ggtgaacgtc cgcggtaact ctgaccgtgt
     5581 taaagtagcg tagtcccttg tcgcgtaagt tgcgacccgc atgaaaggta taacgacctg
     5641 agaactgtct ccacgaatta tccggtgaaa atgtaatgga agtgaagata cttcctacct
     5701 gcggctagac ggaaagaccc cgggagcttt actgcagggt gatattgggt tttggatttt
     5761 gatgcgtagc atagtggtga ggcgttgaga tctcgttttc ggacggggtg gagccgacag
     5821 tgaaatagcc atcttcaata ttcatcatcc taatcctaag ggtgacagtg tcacttgggc
     5881 agtttaagtg gggcgcttgc ctcctaaaaa gtaacggagg cgtttaaagg tcagctaagc
     5941 acggatggaa atcgtgcgtg tcgcgtaaag gcaaaagctg gcttgactgc aaggcggatg
     6001 tgccgcgcag gcgcgaaagc ggaacttagt gaaccgatgc tggttattag aatcggcaga
     6061 gattatcaaa caaaagttac cccggggata acaggctggt acgacccacg agtccatatc
     6121 aacggtcgtg ttcggcacct cgatgtcggc tcatcatagc gcgggggtgg agaagctccc
     6181 aagcgtccgg ctgttcgccg gttaaaatgg tacgtgagct gggttcagac cgtcgcgaga
     6241 caggtcggtc actatctacc gcaggcgttt gagttttgag gagagtcgtc cctagtacga
     6301 gaggaccggg atgaactgac ctctggtgta ccggttgttc cgccaggagc accgccgggt
     6361 agctaagtcg ggaacggata agaactgaaa gcatctaagt tcgaagccgg ctccaagata
     6421 agaactctca gtttcctcca agaacaggag gttgataggc tgcaggtgca agtgcggcaa
     6481 cgcattcagc cgagcagtac taataaacga tcaaacgcct tggttagcaa ttaatgacct
     6541 caaattgttt tttaagattc tggaaaatat aactgacaaa attgaaacaa taccgcattg
     6601 cgcgtattgt aaggaagcta tactgagagt gctccacctg ttcccatccc gaccacagaa
     6661 gtgaaatctc tcaaggccga tgatagtcgc aaggcgaaag taggtagctt tcttacaatg
     6721 cgcgcaattt ttttgtagat caaaaattat aatcgcgagt attttacttt taatttgttt
     6781 tttgttaaat tgacaatatt ggataaagta tgcaaattat atggcaaata atcttgatat
     6841 cgtgagcatg tcgcttccaa agcaattgga cgagcaaata gaaaaagaag caagaacagg
     6901 caaatttgct tcaaaaagcg agtttgtccg ttttttggtt cgtttgtggc aagaggataa
     6961 attggcaaaa gaaattgacg aagcggaaaa ggatattgcc gcgggaagag tcaaagaaat
     7021 aaaatcactg aaagagttaa ggtaaaatat tgtcggatga aaattatccc tacggcaaag
     7081 tttgaacgca attataaaaa gctgtccggt tctctaaaag ataaagcgga aattgctctt
     7141 gaaattttta aaattgattt gtccgattct cgtttgcgtg tccataaatt gcatgggcgt
     7201 tttgcggagt attgggcgtt taccgtcagt tatgattgcc ggattatatt tcgctacggc
     7261 gagaataatg aaattttttt gataaccatt ggcgaccaca aaatatacga ttgaataaac
     7321 tacgccgggg caaagccccg aggtatgctg gcgggtaaat aaatcgcgac ccgacctgaa
     7381 cccatcccga ctccctcgcg aaatccgctc gggaccggcg ccgaagtgaa atctctcaag
     7441 gcccaccttc gttaaaactt cggttgtgat ggaccgaaac gataaatttt gattatgctt
     7501 actcccgaac aggaaaaatg ggttagccat ctttccgaca gcgacaaaat aaaaattcat
     7561 ccttaatgat cagcggcgtt ccggcgcgag cgttgtccac aaaacaaaaa ccgcgaaaaa
     7621 cattcgcggt ttttgttttg attattgaaa gcctaccatt cgccgtcgcc cgcgtcgtcg
     7681 cggtcgtcgt cgtcatcgtc gcccatttgg gcgtcgtcgt cttccaagtc gtcgtcggcg
     7741 aggatgtcgt cgtcgtaatt ttcggcatcc tgcagggcga tggcggtttt aattgtgtcg
     7801 tcaatcatta ttaaatttta attgagaaag atattttttt aaaaaatatc ccgacctttg
     7861 ccgcgaattt tttcgcgata aaatcattgc acatttccat atattattta ttcaaagaat
     7921 tttgtcaata gcaaaaattg cgacttatcc acaggcggca aaaaagaacg gaaaatcaaa
     7981 cgcaacgaaa agaaaaattt ccggcgggtt cgcttttttg cgccgatctg ctataaataa
     8041 gaatagccaa caacagaaac gatgaaagca aatttttcca aaaaatggaa agtggcgctg
     8101 gcgatcaccg gcgttgccgg cgtgattttg gcgttaaaca ttccggcggt caacgcgcaa
     8161 ttcaaaaatg cggcttttgc ggcgctggcg gagatccaga aaaacgtttg ggcggcgggc
     8221 gcgtccgccg gcgaaagctt gccggtgttt gccggcgaaa gccttgccga agagaatcgc
     8281 cgtttacggg cgcaagcgtc cgggttttgg gtgcaacaag cgcaaattga cgcactgaaa
     8341 aaagaaaatg aattcctgcg gcaaggattg aatttggaat tggaaaaaga gtttgatttg
     8401 aagcttgcca atatcgcggg aaaaattctc gcgcgcgaca ttttgattat tgataaaggg
     8461 gcgcacgata tgattgcgcc ggggatgatg gcgattgatt cgcaaaaagc ggtcgtggga
     8521 aaagtgatca aaacctatga taatttttcg gagattcagt taatcacgga caaagacttc
     8581 tcttttgaca tttcaatcgg cgagaacgcc atcggcgggc tttttcgcgg tctgggcgga
     8641 ctgaacgccg aaatcggttt ggtgcccagg gatgccgcgc tggcgcccgg ccagcgggtc
     8701 tacaccagcg ggctgggagg catttttccg gcagggcttc tggcgggtgc agtgaaggaa
     8761 atccgccaaa acgacgttga gactttccaa acggcggcga ttgcgccggc ttttgacgtt
     8821 gccgcggcgc ggcaggtttt tgtggcggtg ggaaaacatc cgatggactc ggcgctgtcg
     8881 gcggaaatta atggcggaaa tgattaagaa actgattttg atcgccgcgg cagtgattgc
     8941 gctgttttta ctgcaaacga gcttcgcgcc tcattttccg ttttttgcgg ggagatggtt
     9001 tgagtgggcg aatttcatta ctttgccggt ggttttgttc gcgctttttg agcggcggcg
     9061 gcacaatttg ggctggggat tggcggtttt ggggggattt ttactggata tttactcggc
     9121 aagatttttc ggtttttgga tgattatttt attgtttgcg gtattggcga tcaaactcgg
     9181 aatcaaaaaa tatgttcggc ttccttcgtt ttggtaaaaa aagaagaata tcgctctctt
     9241 cgccggaaga aattgaaccg caggagatat tttgcgaccg gcgggcacag cggcaaaaca
     9301 agtgcggcga tcaggattgc aaaaaatttg aagtggccgc gcccgccggg gggctggcgt
     9361 tgccggcggt ttttgccgcg ctgttttttt tggccgcgtt tttgcgagcg ggctatctgc
     9421 aaattgttga gggcggtgag cttatgaagc aggcgcagaa aaaccaatat atttttcaaa
     9481 aagtgcagtc cgatcgcggc gtgatttatg acgccggttt caatgttctg gcaaaaaacc
     9541 tgtccgcgtt tgatttgcag tgcgacattc aaaaattttc aaacaatccg gcggagagaa
     9601 aaagaatcat tgaaagcttg gcggcgattt ccggcgcgga cgcggcggcg attgatcagg
     9661 ctgtcggcgc gcaaaagacg gcggttttgg aaaatcttga ccatcagtcg ctgatcatcc
     9721 ttgaggcccg catcaatgat tttcccgggt gccaagtggt tgcccgcccc atcagggaat
     9781 atgttcgcga cgcgggattg gcgcaccttc ttggctatat gagcaaaatc aatcaggacg
     9841 aatggagcgc caacgcagag aattattcca tcaatgatta catcggccgt tcgggattgg
     9901 aaaaatccta cgaaaccgtt ttgcgcaaag acccggggaa attgcgggtt gaacgggacg
     9961 ccaacgggaa cgtgatagcg caggaaatga ccgcgcttcc cgaatcgggc gacagcatcc
    10021 ggctgtggct tgattatgat ctgcagaaaa aattgcgcga ttcaatgaaa aaccaactgg
    10081 aaagcctggg cttgaaaaaa ggcgccgcca tcgcgctgga tcccaaaacc ggaggagtgc
    10141 tggcgatggt ttcttttccg gattacgaca acaatgtctt ttcccagcat aatcgcgagg
    10201 aaatacaaaa attggttgat gacaaggaaa ccaatactct tttaaaccgc gccatcggcg
    10261 gccgttatct taccggttct gccattaagc cgtttatcgc ttcggcggca ttgcaagagg
    10321 ggattatcgc cgccgacaaa aatatcaatt gccaagggag aatatcggtg cagaaccaat
    10381 acgatccgga gattgtctac cattacaacg acaatcatgt ccacgggcaa accaatatgt
    10441 acaaggcaat cgccgaatcc tgcaacgtct acttctacac tattggcggc gggtatgaag
    10501 atcaaaacgg attggggccc acgcgcatca aaaaatattt ggatttgttc ggctgggaag
    10561 aacccaccgg cattgatttg cccggcgaga ttgccggatt tgtgccttcc gccgactgga
    10621 aaaaagaaag attcgccggc acgcaggatc aaaattggac cgacggcaac acctactggc
    10681 tgtccatcgg gcaggagttt atcggtatca cgccgattga agtcgcttcg gcttacgcgg
    10741 cgattgccaa tggcggcacg ctttatcggc cgcagatggt gaaagcggtg gttgacagcg
    10801 caaaaaacac gattgaagag aaaaaaccgg aagtgattcg cgaaaatttc attgatgcgg
    10861 cccatctggc ggcggtgcgg gaagggatgc ggtttgcggt taacggcgcg ggcgcgcctc
    10921 ttgcgtccgc gctcacgctc aaccagctgg ggattaggat gggcgccaag accggcacgg
    10981 cgcagttgta caaagggcct gacggcaagg atttattaaa cagctgggtc aatgtttttg
    11041 ccccctatga cgatccgcag atcgttttgc tggtgatggc cgaagaagtg cgggagggca
    11101 cggtggcggt tttgccggta gcaaaagaag tgctgggctg gtattttgaa cccaagaacg
    11161 aagacggcgt ccgcctttcg gaactgccgc ccgccgaaaa aagcgaaact tccggcgaaa
    11221 ccggcgatat gcttgatatt gaaggcgcaa tgatcaacga aaatcccacc cttcctcccg
    11281 cgaccatccc cgacgaagaa tattaatctt ggaaaataaa acttcgcccg cccaatttgc
    11341 gtttgcatac aaaccccaat tccgaacatt ttttgcggtc gcaaaatttg tcaggaattg
    11401 gggtttgtgt gcaaaaagag ggtaaattac cactcggaag cggcaag
//
LOCUS       DAOQWE010000011        11405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_6129_length_11405_cov_7.613128, whole genome shotgun sequence.
ACCESSION   DAOQWE010000011 DAOQWE010000000
VERSION     DAOQWE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6129_length_11405_cov_7.613128"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..696)
                     /locus_tag="PKK37_00900"
     CDS             complement(157..696)
                     /locus_tag="PKK37_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80972.1"
                     /translation="MVFVVDFSKINIEEILFPEMTGWLLAAKIFFVLFNLGVIAFIVY
                     VWSSTVYLRRLFLWDLVEFFTFRAYNVKLIEKDWNKIKKRLLTQNPAQMKLAMIEADL
                     LVKDVLFRIGYPGKDLAEQLESPKADVFSDIDAMKEADRLYRQIVRDPKIPLDYQTAK
                     TAILSFEQGLKDARAFRNK"
     gene            complement(767..2170)
                     /gene="gatA"
                     /locus_tag="PKK37_00905"
     CDS             complement(767..2170)
                     /gene="gatA"
                     /locus_tag="PKK37_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNR80973.1"
                     /translation="MELKNLTIKDIRGGLMKKEFSARELTAAHLDKIEKENDEIFAFL
                     HIARDLAISQAKAVDEKIKNGEQIGMLEGIPMAVKDNIMVQDLPCTSGSKILQNYIAP
                     YDATVIEKIKGQGAVILGKTNMDEFAMGSSTETSAFGPTRNPRDLRRVPGGSSGGSAA
                     AVAANQCAAALGSDTGGSIRQPACFCGAVGLKPTYGAVSRYGCAAYASSLDQIGPLAK
                     TVADAQIVFDAIRGRDDRDATSAEMPAAKTAKEKYRLGVPKEYFVAGMDPRVESAIKE
                     KISFLEKQGHEIEEVSLPHSEFALACYYIIATSEASANLARYDGMRYGAAKGGDTVTD
                     AYFAARGQGFGAEVRRRIMLGTYVLSAGYYDAYYVRAQKVRSLIRRDFDRALAQVDAI
                     IAPVSPTLPFAIGEKTDDPVQMYLADIFTISVNLAGVPSLAMPCGAADGLPVGLQIIG
                     RHFDESTLFNVGKIIEA"
     gene            complement(2223..2510)
                     /gene="gatC"
                     /locus_tag="PKK37_00910"
     CDS             complement(2223..2510)
                     /gene="gatC"
                     /locus_tag="PKK37_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNR80974.1"
                     /translation="MLSKEETQHIAKLARLGLSEAEIEKYQKDLSAILGYVDKLKSAD
                     IGEAAALAHSTAAENVLREDEAILRPEQKTRRLREAFSEQKDGCLKVKAIL"
     gene            3055..3258
                     /locus_tag="PKK37_00915"
     CDS             3055..3258
                     /locus_tag="PKK37_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80975.1"
                     /translation="MQIEIIARAINHLASMANGASQSNGIPTTPTAQNRSIIPSTCHR
                     GAPQEAFLPKYDMIKPPNISNPI"
     gene            complement(3554..4651)
                     /locus_tag="PKK37_00920"
     CDS             complement(3554..4651)
                     /locus_tag="PKK37_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR80976.1"
                     /translation="MKIWDKLQKLFSEDMAIDLGTANCVVYVQDRGIVMFEPSVVAVN
                     QKTGQILAVGEEAKKMVGRTPAHITAVRPMRHGVISDFEVTEQMFRYFIEKARKRKLL
                     LNLRPRVIVGVPVGVTEVEKKAVIDAGKSAGAREVFLIEEPMASAIGIRLPVQEAQGS
                     FVVDLGGGTTEIAVISLGGIVFGKSLRVAGDKLCEDIMQFVQDEYKLLIGERMAENIK
                     IGIGLAYVGEGAKPAGAAKKEKLIKREFKMRGRNLVTGLPEEIVISENEIRKAIEKSV
                     RQIVNEIKMAIEETPPELIADIMHNGIYLCGGGSQLRGLDELIAKETRMPVKTVEDPK
                     TTVVRGAGMVLENLDTLREVLIETKELEPPK"
     gene            complement(4635..5906)
                     /locus_tag="PKK37_00925"
     CDS             complement(4635..5906)
                     /locus_tag="PKK37_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNR80977.1"
                     /translation="MLQSRLFAKTRKEELPGEMSAGTRLLLRAGFIDQIAAGVYAMLP
                     LGLLTLQKIEKIIAAKMEEAGAQRILLPALVPKKNWETTGRWNSFDVLYKVKSRQEAE
                     YALGATHEEIAAPLVQKFARSYKDFPIAVYQIQTKFRDELRAKSGLLRGREFLMKDLY
                     SFHIDQKDCDSYYEKIKQIYFEIFNAAGVGHAVHLTLASGGSFSKFSHEFQAATLAGE
                     DTIYHCAACGFTVNREIKEDYPKCPECGGQNFSEEKAIEVGNIFKLGTRYSTPFSFEA
                     AGQDGQNLPVLMNCYGIGLTRLMGTAAEISRDDAGLIWPENIAPFFAHLIVLEAKDGE
                     TDEKIRSAAGKIYDDLQKAGIGVLYDDRRQKSAGEKFGDADLLGMPRRLVISEKTIAK
                     DSVEIKKRGGKESKLIKIKEIDNFLKNENLG"
     gene            complement(5919..7001)
                     /locus_tag="PKK37_00930"
     CDS             complement(5919..7001)
                     /locus_tag="PKK37_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR80978.1"
                     /translation="MVATILLSILTLSLLILLHELGHFYFAKKFGVNVEEFGMFLPPR
                     IWGKKIGETIYSINWIPFGGFVKMQGEDDNNQKEPRSFASKPIWQRAVIIAAGVVSFW
                     LISVVILSGVFMSGAVEAVDDNDPAANARVQIISVVADSPAFSAGIKTGDIVKSVRVG
                     GEITPVDRVAQVQEAAADFGGREMILEIERAGNVLEIALTPRENPPAGEGAMGVGLMR
                     TAQKSYSFFSAIGNGAVATWRMTLGVFDGWGQIIGRLVKKEGMPPGAELVGPIGIVGL
                     MSDQAQMGWNYYLQFVAMISVYLAVLNALPIPALDGGRLIFLALEAIRRKPVSEKIEQ
                     SLTAVFYGLLMVLMLFVIVKDIIRLF"
     gene            complement(7151..7708)
                     /gene="frr"
                     /locus_tag="PKK37_00935"
     CDS             complement(7151..7708)
                     /gene="frr"
                     /locus_tag="PKK37_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNR80979.1"
                     /translation="MEYKEIIKKLAPEFDKALQYLDRELAKLHTGRASASLVEDLVVD
                     CFGQKCPLKQLAMISAPEPRQIVIQPWDKSYFEPIQKAIEASQLGLSGAAEGAVIRVS
                     IPDLTGEYRQNLARLIAKTQEDARQAMRRARDDAWSQIQRLERAGEIREDDKFRGKDE
                     LQKLIDDYGKKVDEAGERKKKEIAE"
     gene            complement(7943..8980)
                     /locus_tag="PKK37_00940"
     CDS             complement(7943..8980)
                     /locus_tag="PKK37_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80980.1"
                     /translation="MDAPTAQIVILIFDFYIFNFVIVGHSKQLRYLQAVKDGGLSSHA
                     WIFSGPDKVGKKTAALEWASEILGFSAGEKTAHPDFLFTAPLVDEKTGKSAGEITVAQ
                     IRELIAKMALAPAGKCKITIIDSAHLMNEEAQNCLLKTLEEPPGASLMILIAENDRRL
                     FATVRSRCQIVKFKFLPQSEMEALAEKLAGEIGSRIAAEETKEIAEISFGRPGRLVDF
                     LKNPEELEKWRAAEKEFAGVVRGDLAQKFAYAKKITDDEKEKIKEADAWITLGEILEI
                     WQNHFRRLLLEALNDPQRVRPLLKGSDPLKNFGAQKIAGTLKKIQTLDFELRTTNAHP
                     RLSIENFLLNL"
     gene            complement(9094..10092)
                     /locus_tag="PKK37_00945"
     CDS             complement(9094..10092)
                     /locus_tag="PKK37_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR80981.1"
                     /translation="MKIGIDLGTRNSLVFVPKKGVILQEPSVVAVDLDENKILAVGQA
                     ANEMIGRTPDTIRVYRPMKDGVIADFVVTQAMIRYFINKVSGKFHFFKPELVISVPAG
                     ITSTERRAVIEAGINAGAKAVYLCKEPILSAIGAGIPIHSCSGNMIVDIGGGTTEVAV
                     ISLGGIVNFQSLRVAGDKMDAAIADFVKRKHNLAIGDQTTEDIKMTVGTAISEKDPRT
                     MQIRGRDLITGLPRNVAINANEVSEAVRDSLAEIVQTVKAVLRETPPELSADIMDKGM
                     VLAGGSSLLANLPEFVSNAIGVPCTLAEDALLCVARGTGAVLENLDFYKKSIMVKK"
     gene            complement(10136..11329)
                     /locus_tag="PKK37_00950"
     CDS             complement(10136..11329)
                     /locus_tag="PKK37_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80982.1"
                     /translation="MENSGSGNHIGRDAALAHFSLMFGYKIFSLYYPLFLAAGGLSLA
                     QIGKVYFLIYLPMALGAPLAGFLTRLVHPALMAAVACLGYGAYALAMAFDAQNTVAIY
                     GWQIALGLSAAFFFTALRALMIKNSNGGAEQDFSWFYNASYWAAAAAPVAGAIIVWRC
                     GFEAAFMVSAAICVYAGVLSAGMITHSWETNRRQTALAGFAKSFAKTAKMSFSKKISG
                     YVALAFAVLLAESALHPFFPLFLKTGVGLEQTEVIWFVALVSAAFSVFYFFVLRRGQT
                     GNPGAGVCRGGLIAGVSTLMFGVFLPFLSAVAAFFLEFARNAGGFLCNTGRSALLAKE
                     LKSRPSEAAALDTVFSPLAIALAAVFGGYLVENFGYQCLLLITGTIIAVALAAALIAK
                     KREMR"
BASE COUNT         2823 a         3227 c         2439 g         2916 t
ORIGIN      
        1 tgtcgcgggg cctggaattg caccagggcc tccagcttat gagactggtg agatactact
       61 tctctacccc gcaatgatcg gcttgcgcca atacccgttg attatacttc aatttcgttt
      121 tccagtcaaa caacaagaaa gcgcgggtaa aaacgactac ttgttgcgaa acgcccgcgc
      181 gtcttttaac ccttgctcaa aagaaaggat tgccgttttt gccgtttggt aatccagcgg
      241 aattttggga tcccgcacga tctggcgata aaggcgatcc gcttctttca tcgcatcaat
      301 atccgagaaa acgtcagcct tggggctttc cagctgttcg gccagatcct tgcccggata
      361 gccgatccgg aacagaacat ccttcaccaa aagatcggcc tcaatcatcg ccagcttcat
      421 ctgcgccgga ttttgcgtca acagccgttt tttgatcttg ttccaatcct tctcaatcag
      481 cttcacgtta taggcgcgaa aagtgaaaaa ttccaccaga tcccacaaaa aaagacggcg
      541 caagtacacg gtggacgacc aaacgtaaac aatgaacgca atgactccca aattgaaaag
      601 cacgaaaaaa atcttggccg ccaacagcca tccggtcatt tccggaaaca aaatttcttc
      661 aatgttgatt ttggaaaaat ccacaacaaa aaccaaaagc taaagcgtaa aacgtaaagc
      721 atcaatacaa atttgtattt ctgttttacg ctttatgctt tttgctttaa gcttcaataa
      781 ttttgccgac attaaacaac gttgattcgt caaaatgcct gccgataatc tgcaggccga
      841 ccggcaaccc gtccgccgcg ccgcaaggca ttgccagcga cggcacgccg gcaagattca
      901 ccgaaatggt aaaaatgtcg gccagataca tctgcaccgg atcgtcggtt ttttcaccga
      961 tggcaaacgg cagtgtcggc gacacgggcg cgatgatcgc gtcaacttgc gccagcgccc
     1021 ggtcaaaatc ccgcctgatc agcgatcgga ctttttgcgc ccgcacataa taggcatcgt
     1081 aatatccggc cgacagaaca taagttccca gcattatccg gcgacggact tccgcgccga
     1141 acccctgccc gcgggcggca aaataagcat ccgtaacggt gtctccgcct ttggcggcgc
     1201 cgtagcgcat tccgtcatag cgcgccaaat tggcactggc ttcggaagtg gcaatgatat
     1261 aatagcaggc cagcgcaaat tcgctgtgcg gcaggctcac ctcttcaatc tcgtgccctt
     1321 gtttttccag aaaagatatt ttttccttta tcgcgctttc cacacgcgga tccattccgg
     1381 cgacaaaata ttccttgggc acccccagac gatatttttc tttggcggtt ttggccgccg
     1441 gcatttccgc cgaagttgcg tcgcgatcgt cccggccgcg gatcgcgtca aaaacaattt
     1501 gcgcgtcggc aacggttttg gccagcggcc cgatttggtc cagcgacgac gcataggccg
     1561 cgcaaccgta gcgcgacacc gcgccgtaag ttggcttgag gccaaccgca ccgcaaaaac
     1621 acgcgggctg gcggatggat ccgccggtgt cggaacccag cgccgcggcg cattgatttg
     1681 ccgccacggc cgccgccgat ccccctgaag aacctcccgg aacgcggcgc aaatcccgcg
     1741 gattgcgcgt gggcccgaac gccgaagttt cggtggaact gcccatcgcg aattcgtcca
     1801 tattggtttt gcccagaatc accgctccct gaccctttat tttttcaatc accgttgcgt
     1861 catagggcgc gatataattt tgcaaaattt tggaacccga agtgcacggc aaatcttgca
     1921 ccataatgtt atctttgacc gccatcggaa ttccttccag catgccgatt tgttccccgt
     1981 tttttatttt ttcatcaacc gctttggctt gcgagatcgc caaatcccgc gcgatatgca
     2041 aaaacgcgaa aatttcatcg ttttcctttt cgattttgtc caaatgggcc gccgttaatt
     2101 cccgggcgga aaattccttc ttcatcaatc cgccgcgaat atctttaatt gttaaatttt
     2161 ttaactccat aattcaaaat gtaaaatgcc gttgtatttt ttccactttt taattccaaa
     2221 tttcacaaaa ttgctttcac tttcaaacag ccgtcttttt gctccgaaaa tgcttcgcgc
     2281 aagcggcggg ttttttgctc cggccgcaaa atcgcctcat cctcgcgcaa aacgttttcc
     2341 gccgccgttg agtgcgccag cgccgccgcc tccccgatgt cggcgctttt cagcttgtcc
     2401 acataaccca aaatcgccga aaggtccttc tggtattttt caatttccgc ttccgacagc
     2461 cccagccgcg ccagtttggc gatatgctgt gtttcttcct ttgatagcat atgagtgcat
     2521 cccgccaatg gcaggataat ttttaatttt caattaccaa ttttcaataa attttcaatt
     2581 tttcaatatt caaacaaaaa tcaatactta ataatagccc aaattccgcc ccttgttaat
     2641 cttgacaaaa cgatcttaat ggtattaaat aaattcacta tggaaaagtg ttcatgtggc
     2701 caaaaacttg gcccaaacga aagccatctc catattgaaa atggagactt cgttgctcaa
     2761 acgcctcccg aagaggcgta ccgcgacgaa aatcgacttt aacttttcct tcttttttcc
     2821 tacaacacac gccgagttga ctcggtgttt tggtttttga tttgaatttt aattcgtttt
     2881 ccgattgaaa aatgttataa aataaaaaaa cttcagcatt atctcgagat tcttgagaga
     2941 atacacgaga actgctgaaa ttatgatatt gcccgtataa ccccgagcac tcctcctatg
     3001 aatgatatca ttgttccggc aaaaaatatt agcggataag acctgtacga aattatgcag
     3061 atcgaaatca ttgccagagc gatcaaccat ttggcatcaa tggctaacgg agcatcacag
     3121 agcaatggaa taccgaccac acccactgcc caaaacagaa gcattatccc ttcaacctgc
     3181 cataggggtg ctccacaaga agctttcttg ccaaaatatg acatgattaa acctccaaat
     3241 atctctaatc cgatttaata ctcatcacct acatcttttg gattgctcta ttggcttcaa
     3301 caacacaatg ttccaacgct gatgaactat cctctagccg ctctttttcc attatgcgtg
     3361 aggcaatctc catctgctct cgtgcttcat tgtatactga cggcaataaa tttttatcat
     3421 ttttttatac gcaaaacgcc ccggctttgg ccggggcgtt ttgatatttt aactttgatt
     3481 tgaatttgta ttaagtttta aaattagata tttgaaaatt gtttcggatt tcgtgcttcg
     3541 gatttcggat tttttatttc ggcggttcca gttctttggt ttcaatcagc acttcgcgca
     3601 aagtgtccag attttccagc accatgcccg cgccccgcac caccgtggtt ttggggtcct
     3661 ccacggtttt caccggcatc cgggtttctt tggcgatcag ctcgtccaag ccgcgcagtt
     3721 gactgccccc gccgcacaaa taaatgccgt tgtgcataat gtcggcgatc aattccggcg
     3781 gcgtttcttc aatggccatt ttgatctcgt tgacaatctg gcgcaccgac ttttcaatcg
     3841 ctttgcggat ctcgttttcc gaaatgacga tctcctcggg caatccggtt accaagttgc
     3901 gtccgcgcat tttgaattcc cgcttgatca atttttcctt cttcgcggcg cccgccggtt
     3961 tggcgccttc gccgacatag gccaggccaa taccgatctt gatgttttcg gccatccgct
     4021 cgccgatcag caatttgtat tcgtcctgca caaactgcat aatgtcttcg caaagcttgt
     4081 cgcccgccac ccgcagactt ttaccgaaca cgattccccc caaagaaatc accgcaatct
     4141 cggtggtgcc gccgcccaaa tccaccacga agcttccctg cgcttcctgc accggcaaac
     4201 ggatgccgat cgccgacgcc attggctctt caatcaaaaa cacttcgcgc gcgcccgccg
     4261 attttcccgc atcaatcacc gcctttttct ccacctcggt aacacccacc ggcacgccca
     4321 cgatcacgcg cggccgcaaa ttcaagagca gtttccgctt gcgcgctttt tcaatgaaat
     4381 agcgaaacat ttgttcggtc acttcaaaat ccgaaatcac accgtggcgc atcggccgca
     4441 ccgcggtgat gtgcgcggga gtccgcccca ccattttttt ggcttcttcg cccaccgcca
     4501 gaatctggcc ggttttttga tttaccgcca ccaccgacgg ttcaaacatc acaattcccc
     4561 ggtcttggac gtaaaccacg caattggccg tccccagatc aatggccata tcttcggaaa
     4621 acaatttttg caatttatcc cagattttca tttttcaaaa agttatcaat ctctttaatt
     4681 tttatcaatt ttgattcttt tcccccgcgc tttttgatct caacgctgtc tttggcaatc
     4741 gttttttcgc ttatcaccaa tcggcggggc atccccaaaa gatcggcatc gccaaacttt
     4801 tcgcccgcgg atttttgccg ccggtcatcg tataatacgc cgattccggc tttttgtaaa
     4861 tcatcataaa ttttcccggc ggcgcttctg attttttcgt cggtttcgcc gtccttggct
     4921 tccagaacga tcagatgcgc aaaaaacggc gcgatatttt cgggccatat cagccccgca
     4981 tcgtcgcgcg aaatttccgc cgcggttccc atcagtcggg tgaggccaat gccgtaacag
     5041 ttcatcaaca ccggcaaatt ttggccgtct tggccggcgg cttcaaaaga gaacggtgtg
     5101 ctatatcttg ttcccagctt gaaaatattg cccacttcaa tggctttttc ttcgctgaaa
     5161 ttttggccgc cacattccgg acatttggga taatcttctt tgatttcgcg attaaccgtg
     5221 aaaccgcacg cggcgcagtg gtaaatcgtg tcttcgccgg ccaaagtcgc cgcctggaat
     5281 tcgtgcgaaa attttgaaaa actgccgccg ctggccagcg tgagatgaac ggcatgcccc
     5341 actcccgcgg cattgaagat ctcaaagtag atttgtttta ttttttcgta atagctgtcg
     5401 cagtcttttt gatcaatgtg aaacgaataa aggtctttca tcaaaaattc ccggccgcgc
     5461 aacaatcccg atttggcgcg taactcgtcg cggaatttgg tctggatttg ataaacggcg
     5521 atcggaaaat ctttgtatga ccgcgcgaat ttttgcacca gcggcgcggc aatttcctcg
     5581 tgagtcgccc ccagcgcgta ttccgcttct tggcggcttt tcaccttgta taaaacgtca
     5641 aaactgttcc agcggccagt ggtttcccaa ttctttttgg gaacgagcgc gggcagtaaa
     5701 atcctttgcg cgcccgcttc ttccattttg gcggcaatga ttttttcaat tttttgaagc
     5761 gtcaagaggc ctagcggcaa catcgcgtag actccggcgg caatttgatc aataaatccc
     5821 gcgcgcaaaa gcaagcgcgt ccccgcgctc atctctccgg gcaattcttc ctttctggtt
     5881 ttggcaaaaa gccgtgattg caacatcgtt ttaaaaaatt aaaataagcg gataatgtct
     5941 ttgacgatca caaacaacat caacaccatc agcaatccat agaaaaccgc ggtgagggat
     6001 tgttcaattt tttccgaaac cggtttgcgg cggattgctt ccagcgccaa aaatatcaaa
     6061 cgcccgccgt ccagcgcggg aatcggcagt gcgtttaaca ccgccaaata aaccgagatc
     6121 atcgcgacaa attgcagata ataattccag cccatttgcg cctgatccga catcaagccc
     6181 acgatgccga tcgggccgac caattccgcg cccggcggca tcccctcttt tttcaccaac
     6241 cggccgataa tctgccccca gccgtcaaaa acgccgaggg tcattctcca tgtcgcaacc
     6301 gccccgttgc cgatggcgga aaaaaacgaa tatgactttt gagccgtgcg catcaatccc
     6361 acccccatcg cgccttctcc cgccggcgga ttttcgcgcg gtgtcaaggc aatttccaaa
     6421 acatttccgg cgcgttcaat ttccaaaatc atctcgcggc cgccaaaatc cgcggccgcc
     6481 tcctgaactt gcgccaccct gtcaaccggc gttatttcac cgccgaccct tacacttttg
     6541 acgatatcgc cggttttaat ccccgccgaa aacgccggcg aatcggcaac caccgagatg
     6601 atttgcaccc gggcgtttgc cgccggatcg ttgtcatcaa ccgcctccac cgcgccggac
     6661 ataaaaactc ccgaaaggat cacgacggaa atcagccaga aagaaaccac tcccgcggca
     6721 ataataaccg ctctctgcca aatcggcttt gaggcaaaac tccgcggctc tttttggttg
     6781 ttgtcatctt ctccctgcat tttgacaaat cctccgaaag gaatccaatt gatggaatag
     6841 atcgtttcgc cgatcttttt gccccaaatc cgcggcggca aaaacatccc gaattcctca
     6901 acgttgacgc cgaatttttt ggcaaaataa aaatgcccca attcatggag aaggatcaac
     6961 agactcaaag tcaaaatgct caacaaaata gtggcaacca tctggcataa ttttcagttt
     7021 tcattcatca gttttcaata aatgaggcag tgctccaaca attcaaacaa tcgcaaaaac
     7081 tacgtttgtt tggaaattgg agcaatgaaa atttaatgaa aattgaaaat tgaaaattga
     7141 aaaatttatt ttattcggca atttcttttt tcttgcgctc gccggcttca tccacttttt
     7201 tgccataatc atcaatcaat ttttgcaatt catccttgcc gcgaaacttg tcatcttcgc
     7261 gaatctcgcc ggcgcgctcc aagcgctgga tttgcgacca agcgtcgtcg cgggcgcggc
     7321 gcatcgcctg gcgggcgtct tcctgcgttt tggcaatcaa gcgcgccaaa ttctggcggt
     7381 attcgccggt aagatccgga atggaaacgc ggatcaccgc gccttcggcc gccccggaca
     7441 atcccaactg cgacgcctca atcgcttttt gtatcggctc aaaataggac ttatcccacg
     7501 gctgaataac aatctggcgc ggttcgggcg ccgaaatcat cgccaattgt ttgagcgggc
     7561 atttctgccc gaaacaatcc accaccaaat cctccaccag cgacgcgctg gcgcgcccgg
     7621 tatggagctt cgccaattcc cgatccagat attgcagcgc cttgtcaaac tccggcgcca
     7681 attttttaat tatttctttg tattccataa ataaccgcca agggcggaat taccaatctc
     7741 caactaccaa tctccaaata aattttcaat ggcccaattt ccaaataaat ctgaaaattg
     7801 aaaattagat acttttggaa cttgatgctt ggttcttgga gcctaacatt ttcattttac
     7861 cagatttttc tttattttct actcttgaca aaacttaaaa agaatttttt acttattcat
     7921 tcaatcgctt tttcataaaa ttttacaaat tcagcaagaa gttttcaatg gacaagcgcg
     7981 ggtgggcgtt ggtggtgcgc aattcaaaat ccaaagtttg gatttttttt aatgtgccgg
     8041 cgattttttg cgcaccaaaa ttttttaaag ggtcggaccc tttcaaaaga ggccggaccc
     8101 tttgggggtc atttaaggcc tccagtaaga ggcggcggaa atggttctgc caaatttcaa
     8161 gaatttcgcc cagagttatc catgcgtccg cctcctttat tttttccttt tcgtcatcgg
     8221 tgattttttt ggcataggcg aatttttgcg ccaaatcacc gcgaacaact ccggcaaatt
     8281 ctttttccgc cgcacgccat ttttccaatt cctccggatt tttcaaaaaa tcaaccaatc
     8341 gcccggggcg gccgaaagat atctcggcga tttcttttgt ttcttcggcg gcgattcggg
     8401 agccgatttc ccccgccagc ttttccgcca gcgcctccat ttcgctttgg ggcaaaaatt
     8461 tgaacttgac aatctggcac cgcgagcgca cggttgcaaa aagccgccgg tcgttttcgg
     8521 cgatcaaaat catcagcgac gctcccggcg gctcttccaa agttttgagc aaacagtttt
     8581 gcgcttcctc gttcatcaaa tgcgccgaat caatgatggt aatcttgcat tttcccgccg
     8641 gcgccaacgc cattttggcg atcagttcgc ggatctgtgc cacagtgatt tcgccggcgg
     8701 atttccccgt tttttcgtca acaagaggcg ccgtgaacag aaaatcggga tgtgcggttt
     8761 tttctccagc cgaaaatccc agaatttcgg aagcccattc cagcgccgcg gtctttttcc
     8821 cgaccttgtc cgggccggaa aaaatccaag catgggacga caaaccgcca tctttaacgg
     8881 cttgaaggta tcgtaactgc tttgaatgtc caacaatcac gaaattaaaa atgtaaaaat
     8941 caaaaatcaa aattacaatt tgtgccgtag gcgcatccat cctttggcgg aaaaaattaa
     9001 aaatgttttc gcattttcaa ttttaaactt taaattgtca tttttaattt tgatttttca
     9061 attttaaact gaaacaaaat aaccgcaaag cagttatttt ttgaccatta tgctcttctt
     9121 gtagaaatcc aaattctcca gcaccgcgcc cgtgccgcgc gccacgcaca aaagcgcgtc
     9181 ttcggccaga gtgcagggca cgccgatggc gttgctcacg aattccggca ggttggccag
     9241 caagctggaa ccgcccgcca acaccattcc tttgtccata atgtcggcgg aaagttccgg
     9301 cggcgtttcg cgcaaaaccg ccttgacggt ttgcacgatc tcggccagcg agtcgcgcac
     9361 cgcctcgctc acttcgttgg cattgatagc gacattgcgc ggcaaaccag ttatcaaatc
     9421 acggccgcga atctgcatcg ttcgcggatc tttttcggaa atcgccgtgc cgacagtcat
     9481 tttaatgtct tcggtggttt gatcgccgat ggccagattg tgcttccgtt tcacgaaatc
     9541 ggcaatggcc gcatccattt tatcgcccgc cacccgcaac gattgaaaat tgacaattcc
     9601 gcccagagag atcaccgcca cttccgtggt gccgccgccg atgtcaacaa tcatgttccc
     9661 cgaacaggaa tgaatgggaa tgcccgcgcc gatagccgac aaaatcggct ccttgcacaa
     9721 atacaccgct ttggcgcccg cgttaatgcc ggcctcaatc accgcccgcc tttcggtgga
     9781 agtgatgccc gcgggcacgc tgatcaccag ttccggcttg aaaaaatgga atttccccga
     9841 aaccttgttg atgaaataac gaatcatcgc ctgcgtcacc acaaaatccg cgatcacgcc
     9901 gtctttcatc ggccggtaaa cgcggatggt gtcgggagtg cgtccgatca tttcattggc
     9961 cgcctgcccc accgccagaa tcttgttctc gtccaaatcc accgccacca ccgacggctc
    10021 ctgcaaaatc acccctttct tgggaacgaa caccagcgaa tttcttgttc ccagatcaat
    10081 gccaatcttc atataaaatc caagatttat gatttatgat tctcgtgatt ccaccctatc
    10141 tcatttctct ctttttggca atcaaagcgg cggcaagcgc gactgctatt attgtgccgg
    10201 tgattaaaag caggcattgg tagccgaaat tttcaaccag atagccgccg aaaaccgccg
    10261 ccagcgcaat cgccagcgga gaaaaaacgg tgtccagcgc cgccgcttcg gagggtcggc
    10321 tttttaattc cttggccaac aaagcggagc gcccggtatt gcacaaaaac cctcccgcgt
    10381 tgcgggcaaa ttccaggaaa aacgcggcca cggcggaaag gaacggcagg aaaacgccaa
    10441 acatcaacgt ggaaactccg gcaatcaacc cgccccggca aacaccagcg ccgggattgc
    10501 ccgtttgccc gcggcgcaaa acaaaaaaat agaaaacgga aaatgccgcg gaaaccaacg
    10561 ccacgaacca aatcacttcg gtttgttcca atcccacgcc ggttttcaaa aacaaaggaa
    10621 aaaacggatg caaggcgctt tccgccagca acaccgcgaa cgccagcgcc acatagccgg
    10681 aaattttttt ggaaaacgac attttggcgg tcttggcaaa gcttttggca aatcccgcca
    10741 gcgccgtttg ccgccgattt gtttcccatg aatgggtaat catccccgcc gaaagcaccc
    10801 ccgcataaac gcaaatggcc gccgaaacca taaacgcggc ttcaaagccg caccgccaaa
    10861 cgatgatcgc gcccgcgacg ggtgcggcgg ccgcggccca gtagctggca ttgtaaaacc
    10921 aggaaaaatc ctgttcggcg ccgccgttgg aatttttgat catcagcgcg cgcagggctg
    10981 tgaaaaaaaa cgccgccgac aatcccagtg cgatctgcca tccgtagatt gcaacggtat
    11041 tttgcgcgtc aaaggccatc gccagcgcgt aggcgccata ccccaaacac gcaacagcgg
    11101 ccatcagcgc cggatgaacc aagcgcgtca aaaatcccgc cagcggcgcg cccagcgcca
    11161 tcggcaaata gatcaagaaa taaaccttgc cgatttgcgc caaagaaagc ccgcccgcgg
    11221 ccaagaacag cggataataa agcgaaaaaa ttttgtagcc gaacatcagc gaaaaatgcg
    11281 ccaacgccgc gtcgcgcccg atgtgattgc cactgccgct attttccata attctcaata
    11341 tgcctcatta tgtatttgat tttttcaatt gccagcgttt ccatatcttg atattaccaa
    11401 atcac
//
LOCUS       DAOQWE010000012        11318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_6189_length_11318_cov_7.752553, whole genome shotgun sequence.
ACCESSION   DAOQWE010000012 DAOQWE010000000
VERSION     DAOQWE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6189_length_11318_cov_7.752553"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKK37_00955"
     CDS             <1..190
                     /locus_tag="PKK37_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80983.1"
                     /translation="KNGYQGEFLSQYSYSYHIMGQGVAFTPNMPSASWACTNDIIVPF
                     SEILQLAKPDSILKRMVR"
     gene            356..1786
                     /locus_tag="PKK37_00960"
     CDS             356..1786
                     /locus_tag="PKK37_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR80984.1"
                     /translation="MKNKELQEYVQAQIVSAPKRLRGMANDAQGKPLFKRSVFFELRR
                     YVLDFLAKGGEPRIIVMPGLRGTGKTTLLAQLFLSLDGNVSKFYLSADETIKRFGIGL
                     WDVFENYEELVGKRLEEIDAPLILFLDEIHYDEKWAFFLKSMYDKSKKVMIFCTGSAA
                     LLLREQVGADIARRVFFVDVNPVGFSEYMLFKYGKPPADEVMRAVEDAILRSQSAQDA
                     FEKLQKEGAGIKKYWLDVDSLEISRYVKFGTFPFALKMDNEGMAASFIAQIINKIIYS
                     DIPQFYKFETETLNRIDKMLYLISETLGVSVTKLSQTMEMKPDTLRLILKSLESSGLV
                     SRVAPFGAHFRQVKKPSKYLFATPALRFSYLAARESADVFENYRGSLFEDLAGMYLNG
                     ILPRFGDCSLTYDVSEGGADFIITLGKRRIVMEIGAGKKGYRQAIVTAQKIKPEYSII
                     VSNDELEYSKERNALKIPLKYFLLMR"
     gene            1851..2489
                     /locus_tag="PKK37_00965"
     CDS             1851..2489
                     /locus_tag="PKK37_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80985.1"
                     /translation="MARRKKRNYNFNWIVAGLAVGVFAWIAADFYFNVYRHPQEKVDI
                     VEVADDPETESAPLSGQIAEEGAPAPEGGFVLATYHDDRWGVELRYPTVAGDARCPAP
                     QKNDDGFSLGNFYFFAGGQNEALEDFMARQLQGMEVESRENINTAGRPAVKANYQTPK
                     AGLYGSSVFVENDGRPFEFGLLAKEAAEKCGGADDYDERLYQSVISTLKFAD"
     gene            2586..3068
                     /gene="ybeY"
                     /locus_tag="PKK37_00970"
     CDS             2586..3068
                     /gene="ybeY"
                     /locus_tag="PKK37_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNR80986.1"
                     /translation="MEIDIQIRNLTRRQVNGARIKKAIAAAVNGEKKRFAGEGAEISV
                     VLVGKKRMAAINQKYRGVASATDVLSFAHCEGEELAPGRILGELVICPEIVAQDAKTA
                     GRGFDYQTAWVAIHGALHLLGYDHERGVEQEREMRQKEKYYLSKLKTENEKGKTKIKN
                     "
     gene            3302..4522
                     /gene="ftsA"
                     /locus_tag="PKK37_00975"
     CDS             3302..4522
                     /gene="ftsA"
                     /locus_tag="PKK37_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNR80987.1"
                     /translation="MAKSLLITGLDIGTSSIKIAVAQKRPEEENLEITYAASQPSRGV
                     RRGVVVDAEAAAKCIGESLKAAQLAMGHNLEEVHISVNGGHIFCTRSHGLVSVSRADG
                     SISAEDVDRVLQAARTISLSSNKEIVDIYPREFAVDGESGVKDAVGMKGVRLEAEVLI
                     VAGFTPYLKNSDQAVLRAGLEVANRAPAVIAAAGAALSEQQKELGAAVLDVGAGTTGL
                     AVYEEGDLSHIAILPIGSANITHDIAIGLKVDVETAERLKVEKGICVFKGADKKIKLA
                     GENDKAAGAIFSQRLVSRIIQERVAEIFELANKELKKIGREKKLPGGIVLVGGGVKVP
                     GAEEVAKKVFQLTCAIGAPRNFSNIESDPSLAAVCGLARTGWKEKEEESAGPSRFSAG
                     GLGKIKRFFKIFIP"
     gene            4681..5610
                     /gene="ftsZ"
                     /locus_tag="PKK37_00980"
     CDS             4681..5610
                     /gene="ftsZ"
                     /locus_tag="PKK37_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNR80988.1"
                     /translation="MFNPINIKVAGVGGSGCNAISRMEKDRIRGVELIALNSDYQDLK
                     KVKADVKIQIGKKLTQGLGTGMNPEMGRKAAEEQKEEIQKALAGAGMVFVACGMGGGT
                     GSGAAPVVAEIARNAGALTVAVVTRPFSFEGAYRQRVAQESLDVIKGKVDSLISISND
                     KLLAALNPDTTLANAFWLCDEVLRQAVQSISDLVLSPGIINVDFADVRSILKDSGRAI
                     FGIGRARGEKRAEIAARAAMESPLFDAPARGAKGLLFNVSGGLDISLSEVEEAAKIIT
                     SEINARARVIFGAVHDQTLPKGEIKITVVATGF"
     gene            5879..7999
                     /locus_tag="PKK37_00985"
     CDS             5879..7999
                     /locus_tag="PKK37_00985"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNR80989.1"
                     /translation="MENKITKVQKRDGRIVDFDFDRIVNAIFKAITATGQGDEIRARK
                     MAEQARKLFVRRFKNGSVPGVEQIQDIIEEVLIMEGLVETAKSYILYREQRRRIREAA
                     VASDEAMDRVDQYLEKMDWEVQENANMAFSLQGLNHYAVSYIVKKYWLNKIYPKEIRE
                     ANESGDFHLHNLDTLAPYCSGWDLYDFLRRGFGGTPGKLESKPPKHLRTVLGQAVNFI
                     YTLAGEVAGAVSFSNFDTLIAPFIYYDNLNYHQVKQAMQEFLFNMATPTRVGFQCPFS
                     NVTLDLTPSPSFAKQPVIIGGAPQDKTYGEFQEEMNMLNRAFYEVMLEGDKNQRVFTF
                     PIPTVSITKNFDWDNPAMEPMWEATAKYGVNYFTNYINSDISPEDVRSMCCRLRLDLT
                     ELHNRGGGGLFGSGALTGSIGVVTINMPRLGYLSKTKKEFFQRLERLMDLAKESLELK
                     RKVIEDYIEKGLYPYSRHYLSNIKKSRGQYYGNHFSTIGLVGMNEMLVNFLDENIASK
                     KGRAFALEVMDFMRERMVAYQKETGNIYNLEATPGESTAYRLCLKDKAKYPDIVAAGT
                     KKSPYYTNSSQLPVNFTDDVFEALKLQDEIQCRYNGGTVQHLFLGERLSDTQSVKALV
                     RKIFEKFRLPYITLTPTFSICPTHGYIAGEHFKCPKCAIEQPCEVYTRVVGYLRPVAQ
                     FNAGKQQEYKDRKEYRVKKETLAG"
     gene            8111..8362
                     /locus_tag="PKK37_00990"
     CDS             8111..8362
                     /locus_tag="PKK37_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018639476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNR80990.1"
                     /translation="MEKAIITIDPAVRFGKPCIRGTRIAVADIINLLAAGFAVDEIPG
                     EYPGITKKDVLAALEFSGKMMEKPQHLTEIFLGKVKTVN"
     gene            8363..8728
                     /locus_tag="PKK37_00995"
     CDS             8363..8728
                     /locus_tag="PKK37_00995"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNR80991.1"
                     /translation="MVKIFADHCVGKFFVEPLRQLGADVLTASEAGMERATDEEVFNY
                     ALAEKRILISFDRHFINIARFNIKAGAGVVIFEIERLSKETIKKRIVGFFADKTAAYF
                     KGCIFVVDLAGEINVWPKS"
     gene            8817..9959
                     /locus_tag="PKK37_01000"
     CDS             8817..9959
                     /locus_tag="PKK37_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80992.1"
                     /translation="MIKPQRKFVRVELDKRVIAKFPPFVEYALDSLAPEQEMVIKGGL
                     ARLCCIEALAFANDGFPDQERQAIERRINDVDIIIFHKGTLPKSKDFLVEKFLRIRQN
                     LDAQGIELKSEDAEPVKGEIGEYALRKILCDADLTINESILAPSGGRWVLWTTKECLR
                     DLREGVGFLNAKPAHFRYERGRFMPSNLGWMRLVKFLAEGKISSIYMPRWWREAHFAS
                     MKQKMEAGDLPWGASLGLYSLSLMEKKAGGKPELQSNIMKILNALRFTDIINPDEYIA
                     KQKEGFAAKGQLFELKKFAFEEIIDRAVEKKSQVFDRRHDRNVIKNSCEHFWEQYVCD
                     GCARHCALKLCTQCPAVETMDLPCNDIIARAAWPEDAKSLWRLPRK"
     gene            10149..10913
                     /locus_tag="PKK37_01005"
     CDS             10149..10913
                     /locus_tag="PKK37_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNR80993.1"
                     /translation="MKICGLQKTTLIDYPGHVACTVFTAGCNFRCPWCYSKELVLPEL
                     IARQPEIPEAEVFEFLKEKQGLLEGVVICGGEPTLHPDLPQFCRKIKDLGFLVKLDSN
                     GSNPKMLKELIDKKLIDYVAMDIKLPKDRYAALFAGAARGSTPVKSAGVQPLAIRVDD
                     IEESIKILKGGDVDFEFRTTCAPGVHTPDDFEIMAKWIGQDIPGRSPKYFLQNFKPGR
                     NIDPEFEKLKPFAAEFFAAALAKIKPYVPSVRVRDL"
     gene            11038..>11318
                     /locus_tag="PKK37_01010"
     CDS             11038..>11318
                     /locus_tag="PKK37_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80994.1"
                     /translation="MVPRYDLEKIKFGVDQKTWARAVEMYEAGRVSEVEESYIGFTAR
                     VSGTHPYDVAVSETHYDRGHCNCFVGRQDILCKHMVALAIWAVLRGAPLS"
BASE COUNT         2976 a         2311 c         3147 g         2884 t
ORIGIN      
        1 caagaatgga tatcaagggg aatttttaag ccagtattct tattcgtatc acataatggg
       61 ccaaggagtt gcttttacgc ctaatatgcc ttcggcgtct tgggcgtgta caaatgacat
      121 tatcgttcca ttttcggaaa ttcttcaatt ggcaaaaccg gacagtattt taaaaagaat
      181 ggtgcgatag atagtgagtt gccaacttga ctttcaaaat aaacgggtta aatcgtgagt
      241 taatttttgt tttaaccggg ttaatttgct aaaaaccggt taaacgctca aattactaac
      301 cctttataag ggtatagtat tgacaatcac taaccctttg tggtaggtta ggagcatgaa
      361 aaataaagaa ttgcaagaat atgttcaggc gcaaattgtt tctgcgccga agcgattgcg
      421 cggcatggcc aacgacgccc aaggaaagcc gcttttcaag cggagcgtgt tttttgagtt
      481 gcgccgctac gtgcttgatt ttttggccaa aggcggcgaa ccgaggataa tcgttatgcc
      541 cggattgcgc ggcaccggca agaccacttt attggcccag ctttttttgt cattggacgg
      601 aaacgttagc aaattttatc tttcggccga cgaaacaata aaaagatttg gcatcggttt
      661 gtgggacgtt tttgagaact atgaagaatt ggtcggcaag cgtttggaag aaattgacgc
      721 gccgctgatt ctttttttgg acgaaatcca ttatgacgag aagtgggcgt tttttttgaa
      781 gtcaatgtat gacaaatcaa aaaaagtaat gattttttgc accggatcgg cggcgttgtt
      841 gttgcgcgaa caagttggcg ccgatattgc tcgccgggtt ttttttgttg atgtcaatcc
      901 ggtcggtttt tccgagtata tgctttttaa atacggcaag ccgccggcag atgaggtaat
      961 gcgggcggtt gaagacgcga ttttgcgttc gcaaagcgcc caagacgcgt ttgaaaaact
     1021 gcaaaaagaa ggtgccggaa taaaaaaata ctggctggat gttgattcgc tggaaataag
     1081 ccggtatgta aaattcggga cgtttccgtt cgcgctgaaa atggacaatg aggggatggc
     1141 ggcaagtttt attgcgcaga ttatcaacaa gataatctat tccgatattc cgcagtttta
     1201 caaatttgaa accgaaacat taaaccggat tgataagatg ttgtatttga tttccgaaac
     1261 attgggggtg agcgtgacaa agttgagtca aacgatggaa atgaagcccg acactttgcg
     1321 tctcattttg aagtctcttg aaagttccgg tttggtttcg cgggtcgcgc ctttcggcgc
     1381 ccattttcgg caggttaaaa aaccgtcaaa atatcttttc gcgacgccgg cgctccgatt
     1441 ttcatatttg gcggccagag agagcgcgga tgtgtttgaa aattatcggg gaagcttatt
     1501 tgaggatttg gcgggaatgt atttgaacgg aattttaccg cgatttggcg attgttcgtt
     1561 gacttatgac gtttccgagg gtggagcaga ttttattatt accttgggca aacgcaggat
     1621 tgtgatggaa atcggcgcgg gcaaaaaagg atatcggcag gcaattgtga cggcgcaaaa
     1681 aataaaaccc gaatatagca ttattgtttc aaacgatgaa ttagaatatt caaaagagcg
     1741 caacgcgtta aaaattccgc tgaagtattt tcttttgatg cgataagaaa taagaataga
     1801 cctactgcgt aaatatttac gcagtaggtc taatgcctaa ataaaaaatt atggcgagga
     1861 ggaaaaaaag aaattataat ttcaattgga tagtcgcggg gctggcggtc ggagtttttg
     1921 cgtggattgc cgcggatttt tatttcaatg tctatcgtca tccgcaagaa aaagttgata
     1981 ttgttgaagt tgccgatgat cccgaaaccg aatccgcgcc gctgtccgga caaatcgctg
     2041 aagagggcgc gcctgcgcct gaaggcgggt ttgttttggc gacttatcac gacgatcgct
     2101 ggggcgtgga attgcgctat ccgacggttg ccggcgatgc gcgctgtccg gcgccgcaaa
     2161 aaaacgatga cggtttttcg ctgggcaatt tctatttttt cgcgggaggt caaaatgagg
     2221 cgttggaaga ttttatggcg cggcagttgc aagggatgga agttgagtct cgggaaaata
     2281 tcaataccgc cggccggccg gcggtgaaag ccaactatca aactccgaag gccggcctgt
     2341 acggcagttc ggtttttgtt gaaaacgacg gtcggccgtt tgaattcggg ttgctggcca
     2401 aggaagcggc ggaaaaatgc ggcggtgccg acgattacga cgagcggctt taccaatcgg
     2461 tcatttccac cttaaaattt gccgactgat tgtgtccgat tgcgtccgcg ggcgttgttt
     2521 gttaaaatga atgaggaggc ttacgcaaat aatattattt gtgaaaacct ccttggcgca
     2581 ctccgatgga aattgatatt caaatcagaa atttgacgcg ccgtcaggtt aatggggcgc
     2641 ggatcaagaa ggcgattgcc gcggcggtca acggagaaaa aaaacgtttc gcgggcgaag
     2701 gggcggagat ttccgtggtt ttggtcggca agaaaagaat ggcggcgatc aaccaaaaat
     2761 accgcggcgt tgcttcggct accgatgtcc tatctttcgc gcattgtgaa ggcgaggagc
     2821 ttgctcccgg gagaattttg ggagagttgg tcatttgccc cgaaattgtc gctcaagacg
     2881 ccaaaacggc ggggcgtggt tttgattatc aaacggcatg ggtggcgatt cacggagcat
     2941 tgcatttgct gggatatgac catgagagag gagtagaaca agagagagaa atgaggcaaa
     3001 aggaaaaata ttatctttcc aaattaaaaa ctgagaacga aaaaggaaaa acgaaaatta
     3061 aaaattagac ctactgcgta aatatttacg caacgaaatt ttcagatttc gcgcgagccg
     3121 aatgaggaat gaggaggcat attggcttaa atatgccgac gattgacgaa ttcggcgcag
     3181 ccgaaatatg gaaattgcag taggaaatat ttacgcagta ggtctattaa aaacttttca
     3241 ccggttttat ctttttgatt gtcgttttta tattttcatt tttacttttc aaatattttt
     3301 catggcgaaa tcattattaa tcaccgggtt ggacattggc accagttcaa taaagatcgc
     3361 ggtggcgcaa aaacgccccg aagaggaaaa tttggaaatc acttatgccg ccagccagcc
     3421 gtcgcgaggg gtgcggcggg gagtggtggt ggacgcggaa gccgccgcca agtgcatcgg
     3481 cgaatcgctc aaggccgcgc aattggcgat gggacataat ttggaagagg ttcatatcag
     3541 cgttaacggc ggccatattt tttgcacccg gtcgcatgga ttggtttcgg tttcccgcgc
     3601 cgacggcagt atttccgccg aagatgttga ccgcgtcttg caggcggcca gaacaatttc
     3661 cctgtcttca aacaaggaga ttgttgatat ttatccgcgc gaattcgcgg tggacggaga
     3721 gtccggcgtc aaagatgccg tgggaatgaa gggtgttcgt ctggaagccg aagtgctgat
     3781 cgtggcggga tttacccctt acttgaaaaa ttcggatcag gcggtgttgc gcgccggctt
     3841 ggaagtggcc aatcgcgcgc cggcggtgat tgccgccgcg ggcgcggcat tgtccgaaca
     3901 gcaaaaagaa ttgggagcgg cagtgctgga tgtcggtgcg ggcaccaccg gtttggcggt
     3961 ttatgaagag ggggatttgt cgcatatcgc aattttgcca atcggatcgg ccaacatcac
     4021 ccatgatatt gccatcgggc tgaaagttga tgtggagact gccgaaagat tgaaggtgga
     4081 aaaagggata tgtgtcttta aaggtgcgga caaaaaaatc aagctggctg gtgagaacga
     4141 taaagcggcg ggagcgatat tttcccaaag actggtttcc cgcatcattc aggaacgcgt
     4201 ggcggaaatt tttgaacttg ccaacaaaga attaaaaaaa atcggccgcg aaaagaaact
     4261 tcccggcggc attgttcttg tgggcggcgg cgtcaaagtt cccggtgccg aagaagttgc
     4321 caaaaaagtt tttcagctca cttgcgcaat cggcgcgccc cgaaattttt ccaacattga
     4381 gagcgacccg tcgctggcgg cggtttgcgg gttggcgcgg acgggatgga aagaaaaaga
     4441 agaggagtct gccggccctt cacgattttc ggcgggagga ttgggcaaaa tcaagcgttt
     4501 tttcaagata tttataccat aattaaataa atccgaagca cgaatatcga atatttgaaa
     4561 caattttcaa attatcaaat gatcaaattt ccaaaacgga tttgttttgg aaatttggaa
     4621 gtttgaacat tctaatttgt ttcggatttc ggatttcgga tattcggatt ttcagatgga
     4681 atgttcaatc caatcaatat caaagtggcg ggagtgggag gatcgggatg caacgcgatt
     4741 tcccgcatgg aaaaagaccg gattcgggga gtggagttga tcgcgctcaa ttcggattac
     4801 caggatttga aaaaagtcaa agcggatgtc aaaatccaga tcggcaaaaa acttacccag
     4861 gggttgggga cgggaatgaa tccggaaatg ggccgcaagg ccgccgaaga acaaaaagaa
     4921 gaaattcaaa aggcgctggc cggcgcgggg atggtgtttg ttgcctgcgg tatgggcgga
     4981 ggcacgggtt cgggcgcggc gccggtggtg gccgagattg ccagaaacgc cggcgcgctc
     5041 accgtggcgg tggtgacgcg gccgttttcc tttgagggcg cctatcgcca gcgcgtggcg
     5101 caagaaagtt tggacgttat caagggtaag gtggattcgc tgatttccat ttccaacgac
     5161 aagttgctgg cggcgctgaa tcccgacacg actttggcca acgccttttg gctttgcgac
     5221 gaagtgttgc ggcaggcggt gcagagtatt tccgacttgg tgttgtcgcc ggggatcatt
     5281 aatgttgatt ttgccgatgt gcgttccata ttaaaggatt ccggacgggc gatattcggt
     5341 atcggtcgcg cccgggggga aaagcgcgcg gaaatcgcgg cgcgggcggc gatggaatca
     5401 ccgctgtttg acgcgccggc gcgcggcgcc aagggtttgc tgttcaatgt ttccggagga
     5461 ttggatatca gtttgtccga ggttgaagaa gccgccaaga tcatcacttc cgagatcaat
     5521 gcgcgggcgc gggtgatttt cggcgcggtg cacgatcaaa ctttgcccaa gggggagatc
     5581 aagatcacgg tggtggccac cggcttctaa aactgaattt caaattctaa attctaaata
     5641 aattcagaat ttaaaaatca aaacaaaaaa agtatttttt taaatttgaa ttttagattt
     5701 gtttagggtt tagaaattag ggtttagaat tttttaaggg ggatttgacg gattttttgg
     5761 gtgggctaaa atggggtatt gtctttgtta aaatctctga ttgccggttt ataaacgaat
     5821 atgagccgat tttaaaatcg tttcaatttg ccgataaacg cggaagaata aaaaagccat
     5881 ggagaataaa atcaccaaag tgcaaaaacg ggatggccgg atcgttgact ttgattttga
     5941 ccggattgtt aacgcgattt tcaaggcgat taccgccacc ggacagggcg atgaaatccg
     6001 agccagaaag atggccgagc aggcgcgcaa attgtttgtc cgccgtttta aaaacggcag
     6061 tgtgcccggc gtggagcaga ttcaagacat cattgaagaa gtgttgatta tggaagggtt
     6121 ggtggaaacc gccaaatcct acattctcta ccgcgagcaa aggcggcgga tccgcgaggc
     6181 ggcggtggcc agcgacgaag cgatggaccg ggtggaccaa tatctggaaa aaatggattg
     6241 ggaagttcag gaaaacgcga atatggcctt ttctttgcag gggcttaatc attacgcggt
     6301 gtcctacatc gtgaagaaat actggctcaa caaaatctat cccaaggaaa tccgcgaggc
     6361 caatgagtcg ggcgattttc atctgcacaa tttggatacc ttggcgcctt attgttccgg
     6421 ctgggatctc tacgattttc ttcgccgcgg gttcggcggc acgccgggga aactggaatc
     6481 aaagccgccc aagcatttgc gcacggtttt ggggcaggcg gtgaacttta tctacaccct
     6541 ggcgggcgaa gtggcgggcg cggtaagctt ttccaatttt gacacgttaa ttgcgccgtt
     6601 tatttattac gacaatttga attatcatca agtcaagcag gcgatgcagg aatttctgtt
     6661 caatatggcc acgcccacgc gggtgggatt tcaatgtccg ttttccaacg tgacgttgga
     6721 tttgacaccg tcgccgtcgt ttgccaaaca gccggtgatc atcggcggcg cgccgcaaga
     6781 taaaacctat ggcgaattcc aggaagaaat gaatatgctc aaccgcgcct tttacgaggt
     6841 gatgctggag ggcgacaaga atcagcgggt gttcactttt ccgattccga cggtgagtat
     6901 caccaaaaat tttgattggg acaatccggc gatggagccg atgtgggagg ccacggccaa
     6961 atacggcgtc aattatttca ccaattacat caattccgac atcagccccg aagatgtgcg
     7021 ctcaatgtgc tgtcgcctgc ggcttgatct caccgaattg cacaatcgcg gcggcggcgg
     7081 gttgttcggc agtggcgcgc tcaccgggtc catcggggtg gtgaccatca atatgccgcg
     7141 gctgggctat ctttcaaaaa ccaagaaaga gttttttcag cggctggaac gcttgatgga
     7201 tttggccaaa gagagtttgg aattgaaacg taaggtgatt gaggattata ttgaaaaggg
     7261 gctgtatcct tattcccgcc attatttgtc caacatcaaa aaatcccgcg gccaatatta
     7321 cggcaatcat ttttccacca tcggattggt ggggatgaac gagatgctgg ttaattttct
     7381 ggatgaaaat atcgcttcca aaaaggggag ggcgtttgcc ttggaagtga tggattttat
     7441 gcgtgagcgg atggtggcct atcaaaaaga aaccggcaac atttataatc tggaagcaac
     7501 ccccggcgaa tccaccgctt atcgcttgtg cttgaaagac aaggccaaat atcccgacat
     7561 tgtcgcggcg ggcacaaaaa agagccccta ctacaccaat tcatcgcaat tgccggttaa
     7621 tttcaccgac gatgtgtttg aggcgttgaa attgcaggac gagatccagt gccgctataa
     7681 cggcggcacg gtccagcatt tgtttttggg ggagagattg tccgatacgc agagcgtcaa
     7741 ggcgctggtg aggaagatct ttgaaaaatt ccgtttgccc tatatcacgc tcacgccgac
     7801 attttccatt tgccccacgc acggctatat cgccggcgaa catttcaaat gtcccaaatg
     7861 cgccattgaa cagccgtgcg aagtctacac tcgcgtggtg ggctatttgc ggccggtggc
     7921 gcaattcaac gccggcaaac agcaggaata caaagaccgc aaagaatacc gcgtcaaaaa
     7981 agaaaccctg gcggggtagt tattagttat cagttgttag ttgtcggaga ttggaggcaa
     8041 aaattcaccc cggcttagcc cggggttgtt cgaaattgac caaatttgtc gcgcgtgttt
     8101 aaattaagat atggaaaaag cgattatcac cattgatccg gcggtgcgtt ttggcaagcc
     8161 atgtattcgc ggcaccagga tcgccgtggc cgacatcatt aatcttttgg cggcgggctt
     8221 tgccgttgat gaaattcccg gtgaatatcc cgggatcact aagaaagatg tgctggcggc
     8281 gcttgagttt tccggcaaaa tgatggaaaa gccgcaacat ttaacggaaa tttttctcgg
     8341 taaagttaaa actgtcaatt agatggtgaa aattttcgcc gatcattgcg tgggtaagtt
     8401 ttttgttgaa ccgttgcggc aattgggagc ggatgtttta acggcaagcg aagccggaat
     8461 ggagcgggcg accgatgaag aagtttttaa ttatgctttg gcggaaaaaa gaattttaat
     8521 cagttttgac cgtcacttca tcaatattgc gcgctttaat atcaaagctg gcgcgggagt
     8581 ggttattttt gaaattgaac ggctcagtaa agaaacgatc aaaaaacgca tcgtggggtt
     8641 ttttgccgat aaaaccgccg cttattttaa aggatgtatt tttgtggttg atttggcggg
     8701 cgaaataaat gtttggccga aaagttaacg gcaaacagca ggaatacaaa gaccgcaaag
     8761 aataccgcgt caaaaaagaa accctggcgg ggtagttgcc gccggccaaa tcttttatga
     8821 taaaaccgca gagaaaattc gtgcgggtgg aattggacaa acgagtaatc gccaaattcc
     8881 cgccgtttgt tgaatacgcg cttgattcgc tggcgccgga gcaagagatg gtgataaaag
     8941 gcgggcttgc ccgcctttgt tgcattgagg cgctggcgtt tgccaatgat ggattccccg
     9001 atcaagaacg gcaagccatt gagcgccgga taaacgatgt tgatattatt atttttcaca
     9061 agggcacttt gcccaaaagc aaggattttt tggtggaaaa atttttgcgc atccggcaga
     9121 atcttgacgc tcaagggatt gaactgaaaa gcgaagacgc cgaaccggta aaaggggaaa
     9181 tcggcgaata tgctttgcgt aaaattttgt gcgacgccga tttgaccatt aatgaatcga
     9241 ttctggcgcc cagcggcggc cggtgggtcc tttggaccac caaagaatgc ctccgcgatc
     9301 tcagggaggg ggtggggttt ttgaatgcca agccggcgca cttccgttat gaaaggggcc
     9361 ggtttatgcc cagcaatttg ggctggatga ggctggtaaa atttttggcc gaggggaaga
     9421 taagttcaat ctatatgccg cgctggtggc gggaggcgca tttcgcgtca atgaaacaaa
     9481 agatggaggc cggcgatctg ccgtggggcg cgtcgcttgg tttatattca ctgtcgttga
     9541 tggaaaagaa agccggcgga aaaccggaat tgcaaagtaa cattatgaag attctaaacg
     9601 cactgcgttt taccgatatc atcaatccgg acgaatatat tgccaagcaa aaagaagggt
     9661 ttgccgccaa gggccagctt tttgaattga agaaattcgc ttttgaagag atcattgatc
     9721 gggcggttga aaagaaaagc caagtttttg accggcggca cgaccgtaat gtgatcaaaa
     9781 attcgtgcga gcatttttgg gagcaatatg tttgcgacgg ctgtgccaga cattgcgcgc
     9841 ttaaattatg cacccagtgt ccggccgtgg aaaccatgga tctgccctgc aatgatatta
     9901 tcgcccgcgc cgcctggccg gaagatgcaa aatccctatg gcgcttgcct cgcaaatgag
     9961 ttttgccttt tccgccggcc gcattatctg acaaattttg cgaccgcaaa atttgtcaga
    10021 ttttccaatc cgttccatct tgtttcatcc atcctgaaat ccgttcggat gatatttgcg
    10081 gctcttggtt aaattggcgg gcggggttat aattgacaat cgcgggaaat tattcaagat
    10141 aaatcattat gaaaatttgc ggactgcaaa aaacaacgtt gattgattat ccggggcatg
    10201 tggcgtgcac cgtgttcacg gccggatgca atttccgctg tccgtggtgt tattccaaag
    10261 aattggttct gccggagctg attgcccgcc agcccgaaat tccggaagcg gaggtttttg
    10321 aatttttgaa agaaaaacaa ggattgttgg aaggggtggt gatttgcggg ggagagccga
    10381 cattgcatcc ggacttgccg caattttgcc ggaaaataaa ggatttggga tttttggtga
    10441 aattggattc caacggttca aatcccaaaa tgctgaaaga attgattgat aaaaaattga
    10501 ttgattacgt ggcaatggac atcaaactgc ccaaagatcg ctacgcggca ctttttgccg
    10561 gcgccgccag gggttcaacc cctgttaaat ctgcaggggt tcaacccctg gcgataagag
    10621 tggatgatat tgaggaaagt ataaagattt tgaaaggcgg tgatgttgat tttgaatttc
    10681 gcaccacttg cgcgcccggg gtccatacgc ccgatgattt tgagattatg gcaaagtgga
    10741 tcgggcaaga cattcccggc cgatcgccca aatatttttt gcagaatttc aaacccgggc
    10801 gcaacattga tcccgaattt gaaaaattaa agccctttgc cgccgaattt tttgccgcgg
    10861 cgctggccaa gattaaaccc tatgtgccgt ccgtccgcgt ccgcgacttg tgaacaataa
    10921 cagacctgcc aatcaataat tgatgattag cggacattcc aacattcttg agaatgtgag
    10981 aatgtttaaa tgtttggatg ttttaattaa aagggtccga cccttttaaa atgttttatg
    11041 gtgccgcgat atgatttgga aaaaattaag tttggggtgg accaaaaaac ttgggcgcgg
    11101 gcggtggaga tgtatgaggc cggcagagtg agcgaggtgg aggagtccta tatcggtttt
    11161 accgcgcggg tttcgggcac tcatccttat gacgtggcgg tttccgaaac gcattacgat
    11221 cgcggccatt gcaattgttt cgtgggccgg caggatattc tttgcaaaca tatggtggcg
    11281 ctggcgattt gggcggtttt gcggggcgcg ccgctgtc
//
LOCUS       DAOQWE010000013        10918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_6448_length_10918_cov_8.119212, whole genome shotgun sequence.
ACCESSION   DAOQWE010000013 DAOQWE010000000
VERSION     DAOQWE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6448_length_10918_cov_8.119212"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..409)
                     /locus_tag="PKK37_01015"
     CDS             complement(152..409)
                     /locus_tag="PKK37_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HNR80995.1"
                     /translation="MAQKANSAFMKPMNVSDTLAVVVGKGPMPRSEVVKKLWEYIKKN
                     GLQDPKNKRNINADANLQAVFGGKKVVNMFEMTKLVSAHLS"
     gene            815..1441
                     /locus_tag="PKK37_01020"
     CDS             815..1441
                     /locus_tag="PKK37_01020"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNR80996.1"
                     /translation="MATNTGEISEKTAAMKQIKDEVVACRKCALCEFRRQNNYFPVIG
                     QGNHCAKIMFVGEAPGTNEAKTGRPFCGAAGKILDELLSSAGIEREEVYIGNVLKDRP
                     PGNRNPQPEEIAACTPYLERQIEIINPEVIGALGNFSAAFLLEKYGLKDKIQGISKIH
                     GQIFETGPEFGGKIIVPLYHPAVAVYNANMKEILKNDFQILARQIGKN"
     gene            1449..>2020
                     /locus_tag="PKK37_01025"
     CDS             1449..>2020
                     /locus_tag="PKK37_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80997.1"
                     /translation="MEKEKIKKIFIGSALAITVLAAGAALVILFPLVFLLEENKLVIE
                     NELNQTREGFSVFAGNLLLKIDGLERALKQTERDLAIASAQRDYLGLQYNDAMEKYLQ
                     EKARMDDLHSQVGQIQGSVQTLEKLKTIDPELLKKYSKIYFLNENYIPESLAKIDSEY
                     VFNPEEDYLFHAKAWPFLKVMMATAVADGI"
     assembly_gap    2021..2120
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2121..2572
                     /locus_tag="PKK37_01030"
     CDS             <2121..2572
                     /locus_tag="PKK37_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNR80998.1"
                     /translation="AWPFLKVMMATAVADGIDIKIISAYRSFGEQTAIKASYKMVYGS
                     GANQFSADQGYSEHQLGTTIDFTDSEVGASFSGFEKTKAYRWLEDNAHQYGFILSYPD
                     DNHYYQYEPWHWRFVGRDLAQKLRAEEKHFYDADQREIDEYLISFFD"
     gene            complement(2639..3211)
                     /locus_tag="PKK37_01035"
     CDS             complement(2639..3211)
                     /locus_tag="PKK37_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR80999.1"
                     /translation="MKTKYALTAIAVVLCFVLSTAVSATVPAGNQYGNATSSAAAGNG
                     NASRLEALQKQYERLAAMIAELKAKLGQGSASVVADQAQLDCARAAVSARESAIATAQ
                     DAFGNCTATALSARKAALESAWNIAESRERNKATNDAWNKFTQDQKTCRENHKKTVKA
                     AWDEFKSAIKNCKIANGEGNREGIDLSLGN"
     gene            3351..3638
                     /locus_tag="PKK37_01040"
     CDS             3351..3638
                     /locus_tag="PKK37_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81000.1"
                     /translation="MEAAIKRNVVKTVETETITQEQFLAEHNKLSPSNLRANGDLLLR
                     FRQEKSSLFKEDGWSLEKLRRPFIIWLTAQSFYEKSKDEVTVNNQKYEKFS"
     gene            3879..4373
                     /locus_tag="PKK37_01045"
     CDS             3879..4373
                     /locus_tag="PKK37_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNR81001.1"
                     /translation="MKKVYIYPGTFCPPHHGHAALANRAAAAFGNVLVICSVNPVKKE
                     KNIFTPEECKKMWQTYGLRRGVKIATLDEFLKKRNQNDMTVMIRGIRDNNDIIYENAV
                     MIYNRDNFGVNHYHYIIGDKEFEKFSSTAVRAAAESGNQEKLNKLVNPQIAALILAKF
                     RRKN"
     gene            4398..5372
                     /locus_tag="PKK37_01050"
     CDS             4398..5372
                     /locus_tag="PKK37_01050"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNR81002.1"
                     /translation="METTKRETAKDFFLHLLSAATLYMSVIGFIALWWQYINFLFPDK
                     LNPMGMYAGRYETLLWATSMLAVSYPVYVLVSWIIGRGFKTDLAGREVKIRKWLWYIT
                     LFIAAMTMIIDSVTIVFNFLKGDLTIQFFLKLLVILATAAAVFGYYLWDLKKREIVSN
                     LPKKLAWIVGAVVLASIGYGFVLAGSPAKQRDIRFDERRVNDLQNLQYAIVDFWQSRQ
                     ELPAELSELDVLGYAMPADPATGESYGYTATGDLSFELCAEFAAEFAIDEGKDRYSYY
                     TGPQGVATLPKGWGHKAQRTCFESVIDKDFFESQMNYKPDAISAPIRD"
     gene            5596..6831
                     /locus_tag="PKK37_01055"
     CDS             5596..6831
                     /locus_tag="PKK37_01055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR81003.1"
                     /translation="MQTKERSGGFGGYFMKSRSFSVGDRRPRYRAPKNFGAKKRGFGG
                     IKIDPAKFVNKAVMVDGNGGERMNSAKFADFSIDEILKRRIAAVGYQNATPIQELAIP
                     PILSGADVVGVADTGTGKTAAFLVPLINKVLSNRRMRILIVAPTRELAAQTDEEFKKL
                     AQGMKMFSACCVGGASVGRQLAQLRYFNNFIIGTPGRLKDFIDRKRINLAEFSAIVLD
                     EADRMMDMGFIADMRFLMAGMPKERQTLFFSATLSPEIKRLIGEFLREPVFISIKTRD
                     TSKNVEQDVVKVAGRNKVDLLREMLVREEFKKVLIFGRTKFGVEKLCKTLEKSGIKAE
                     SIHGDKKYSRRVKALETFKSDRVQVLVATDVAARGLDIAHVTHVINYDLPQTWEDYVH
                     RIGRTGRGANFGKALTFID"
     gene            6901..7773
                     /locus_tag="PKK37_01060"
     CDS             6901..7773
                     /locus_tag="PKK37_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81004.1"
                     /translation="MENLENQKIETLDYYVRQTLDNLPKWEYPKLEIKKNCRNVFVGS
                     GNAANAGRILAREFGGAGFSVVDYQSFFKNCDDSDRDVYIINASGGKDGVKMAQWLTQ
                     NGRRPKLITSNPEPPAGKFLRVEDIFAFPAIKEPPTYNVSTYAAMLYGILKEDISGAD
                     ERIKNLKVPDLRRYKFIFFLTDDKYEPAGRVAKRKVAESLAGLGADAGGHSNAAHGML
                     LQPNADRLIVCINCDYEGEGDWWRFDAGSYIEAILAVHYIVGKNQTTADSQNILRNYF
                     ENAKKQGWEFNKVW"
     gene            7798..8265
                     /locus_tag="PKK37_01065"
     CDS             7798..8265
                     /locus_tag="PKK37_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNR81005.1"
                     /translation="MDKKVLIILVFAAVIFIAGIVASFPRKKGSAVEINAPRVCLNGF
                     CYEVEVADNPTDRARGLMFRESLDENEGMLFVFDREEKHSFWMKNTLISLDIVWMNSA
                     GEIVEISENTPPCVADSCPSYVPEEPAKYVLEISAGQTQKTSVRIGNRAIIEF"
     gene            complement(8269..10377)
                     /locus_tag="PKK37_01070"
     CDS             complement(8269..10377)
                     /locus_tag="PKK37_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNR81006.1"
                     /translation="MQNEPLDGFFNPQTIAVIGASDRPHSVGGAIFSNIKNGTFAGKV
                     FPVNNKNAAVQGQKAFPFVGDIGEKVDLAVIAAPAPAVLPILDDCARAGAGGAVIVSS
                     GFKESGKQGKKDFSAIQKLARSSGMRILGPNCLGFANPRAGLNASFAPQSPLPGSIAF
                     ISQSGALCGTLLDWSLRDNIGFSHFVSIGSAADIDFAEMIEYFSHDENVGSILLYMES
                     LNDGPRFIAAARDFCQTKPIVCLKAGANSAGAAAAASHTGAIAGNDKVFSAVFERAGV
                     IRAATISEFYNYARTLNHCKKPQGRRLAIITNAGGSGVISTDFLAQNGGELSKLATKT
                     IKTLASTLPPAASRANPIDVLGDGAPEHYRAAVSACLKDKNVDGIMAIISPQAVTQSK
                     KIAEGIAGIEDIAAKPVFISFMGAARVAEGVEIFRRAGIPTYRTPEKAIACFLGLAKW
                     QKDLKRAKKAFSRVRFDFDSAKNTAAEVFAAAAEKKQSIIDGEAARKILQAYGLPGNP
                     AHPAKTAGQAATIAKKIGFPVALKLQSGLAHKTELGGVRLNLDTAAEVKKAFDEIEKN
                     AEKHLRAKDFREITVEKMVDKKYELIVGAKIDPLFGPVIVFGAGGTAVEIFDDTAIGL
                     PPLDAAGAKELMAKTKIFKLLKGYRNIKGADIEELAGVMARFSRLPLDFPQIKEIDIN
                     PLAVDQTGIVALDAKIIPEK"
     gene            complement(10706..>10918)
                     /locus_tag="PKK37_01075"
     CDS             complement(10706..>10918)
                     /locus_tag="PKK37_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81007.1"
                     /translation="TSSINAFQFNTRPMKNKTGNQKAGNPYGDILIETDRKRQTLFSG
                     GFSPPIEKQSIFPAPTRAGCFNYITP"
BASE COUNT         2746 a         2408 c         2836 g         2828 t
ORIGIN      
        1 ctttgatatc caaacagctc cgttttggca gtaaaacacc cttacaaact tgagttttta
       61 aggatgtgtt aatggcgggg gatgttgtgg cgattaataa aacaaaaaat ccgcgaaagc
      121 ggattttttg cgcaagattt gattggataa actacgagag gtgggcggaa accagtttgg
      181 tcatttcaaa catattgacc actttcttgc ctccaaacac cgcctgcaag ttcgcatcgg
      241 cgttgatgtt gcgcttgttc ttcgggtctt gcaaaccgtt tttcttaata tattcccaaa
      301 gttttttcac gacttcggaa cggggcatcg gtcccttgcc gacgacgacg gctaacgtat
      361 cgctcacatt catcggcttc ataaaagccg aatttgcttt ttgtgccata ttttattttt
      421 ccgccgtgtt aacgcggtcg gaattattat tttcaatcct ttatggaaag atagcataaa
      481 tttgctttgt gtccaatagt ttttccccaa gtgtcatttt ttccaattgt tcccgatttg
      541 gttaaaacgg cgttgccgga gcctaccttc catatttgtt gtgcggtgcg accatcatgt
      601 tgctgccggg tgcgcaatag taaccattca ctggggattg tattttgagg gtctatccct
      661 cgtgcttgtc ccgaggtaat ccgagggata ctcgcgagcg gttattagac cctggtaaaa
      721 gaattttaaa ctcagcgagt gatcaccgtg cgatgctact tacgcgattt ttattttggt
      781 gtatgataga acaatagtca ataacgatga atttatggcg acgaacacag gtgaaatttc
      841 ggagaaaacg gcggcgatga agcaaatcaa ggatgaggtg gtggcctgcc gaaaatgcgc
      901 gctttgcgag ttccgccggc aaaacaatta ttttccggtg atcggacagg gaaatcattg
      961 cgccaaaatt atgtttgtgg gcgaggctcc cggaacaaac gaagccaaaa ccggccgacc
     1021 gttttgcggt gcggcgggaa aaattttaga cgagcttttg tcgtcggcgg gaatcgaaag
     1081 agaggaggtt tatatcggca acgttcttaa ggaccggccg ccgggcaatc gcaatcccca
     1141 gccggaagaa attgccgcct gcacgcctta tttggagcgg cagattgaaa tcatcaatcc
     1201 cgaagtaatc ggcgccttgg gaaatttttc cgcggcgttc ttgctggaaa aatacggact
     1261 gaaagataaa atacaaggca tcagcaaaat ccacggccag atttttgaaa ccgggccgga
     1321 gtttggcggc aaaattatcg tgccgcttta tcatcccgcg gtggcggtct ataacgccaa
     1381 tatgaaagaa atcttgaaaa atgattttca aattttagcg cgccagatcg gcaaaaatta
     1441 aaaatgatat ggaaaaagaa aaaataaaaa aaatattcat cgggtcggcg ctggcaatta
     1501 ccgtgctggc ggcgggcgcg gcgctggtaa tcttgttccc gctggttttt ttgctggaag
     1561 agaataagct cgtcattgaa aacgaattga atcaaaccag ggaggggttt tccgtgtttg
     1621 ccggaaattt attgttaaag atcgacggac tggaaagggc gcttaaacaa accgagcgcg
     1681 atttggcgat tgcttccgcc cagcgtgatt atttgggatt gcaatataac gacgcgatgg
     1741 aaaaatacct ccaagaaaag gcgcggatgg acgatttgca ctcgcaggtc ggccagatac
     1801 aaggatcggt gcagactttg gaaaaattga aaaccattga tccggagctc ttaaaaaaat
     1861 attccaagat ttatttttta aacgagaatt atatccccga atcgctggcg aagattgact
     1921 ccgaatatgt tttcaatccc gaagaagact atctttttca cgccaaagcg tggccgtttc
     1981 tgaaagtgat gatggcgacg gcagttgccg acggcattga nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn aagcgtggcc gtttctgaaa gtgatgatgg cgacggcagt
     2161 tgccgacggc attgatatta aaatcatctc cgcttaccgg tcgtttggcg agcagacggc
     2221 gatcaaggct tcctacaaaa tggtttacgg atcgggcgcc aaccaatttt cggcggatca
     2281 gggttattcg gagcatcaat tgggcaccac gattgatttt accgattccg aggttggggc
     2341 aagtttttcg ggttttgaaa aaacaaaagc ttaccggtgg ttggaagaca atgctcacca
     2401 gtatggtttt attctttcgt atccggatga taatcattat tatcaatatg aaccgtggca
     2461 ctggcggttt gtgggccgag atttggcgca gaaattgcgc gccgaagaaa agcatttcta
     2521 tgatgccgac cagcgcgaga ttgatgaata tttgatttca ttttttgact gacggcccgg
     2581 cggcggcgcg aaaacaaaaa gagcggccgg taggccgccg tttttgtttt cgcaattctt
     2641 aattgcccaa tgacagatca atgccttcgc ggttgccttc gccattggcg atcttgcaat
     2701 ttttgatcgc gctcttgaat tcatcccagg cggccttcac cgtttttttg tgattctccc
     2761 ggcaggtttt ttggtcttgg gtgaatttat tccaagcgtc atttgtagcc ttgttgcgtt
     2821 cgcggctttc ggcgatgttc cacgcggatt caagcgcggc ttttctggcg gacaacgccg
     2881 ttgctgtgca attgccgaat gcgtcttgtg cggtcgcgat tgcgctttcc cgcgccgata
     2941 ccgcggcacg cgcgcaatcc aattgagctt gatcggcgac cactgatgcg gagccttgcc
     3001 ccagcttagc ttttaattct gcaatcatcg cggccagccg ttcatattgt ttttgcaacg
     3061 cttccaggcg cgacgcgttg ccgtttccgg ccgcggcgct tgaagtggca ttgccatatt
     3121 ggtttcccgc cggcactgtt gccgaaaccg cggtggataa aacaaagcac aaaacgaccg
     3181 caatggccgt taaagcatat ttggttttca ttttgcggag cgataagtta tttttaagcg
     3241 gactatcgac ctttgttttt tctattacca ttataataaa ccatataaaa aaatctattc
     3301 acaccacatg atttgcttgc catttggcga taatagtgta gggttaaggt atggaagcgg
     3361 caatcaagcg gaacgtggtt aaaaccgttg aaacggaaac aatcactcaa gagcaattct
     3421 tggccgagca caataagttg tcgccgagca atttgcgggc gaacggcgat ttgttgttgc
     3481 gctttcgcca agaaaaatcc tctcttttca aagaggatgg ctggtcgttg gaaaaattgc
     3541 gccggccgtt tattatttgg ctcaccgcgc aatcttttta tgaaaaatca aaagatgaag
     3601 tgacggtcaa caatcaaaaa tatgaaaaat tctcataatc ccggcaagaa caagaataag
     3661 accgcccacc gccgtctggt taaaaaacat cgccccaata aatatcgtcc ggcggcaaag
     3721 cgctaggccg gaaaatcttc cgttaacaga gatcgctttt ggagcgattt tttgttataa
     3781 tcaagctatc ttcgttgaat tgcgattgtt taagcgacga tctctaagga gggcgttgcg
     3841 cttgccattg cttgtcaaaa agattttgcc aaacaattat gaaaaaagtt tatatttatc
     3901 cgggaacttt ttgtccgccg caccacgggc acgcggcgct ggcaaatcgc gccgccgccg
     3961 cgtttggcaa tgttttggtg atttgttcgg ttaatcccgt taaaaaagag aaaaacattt
     4021 tcactcccga agaatgcaaa aaaatgtggc aaacatatgg attgcgcaga ggggttaaaa
     4081 tcgccactct ggatgaattt ttaaaaaaaa gaaaccaaaa cgatatgaca gtgatgattc
     4141 gcggcattcg cgacaacaac gacattatct atgaaaatgc ggtgatgatc tataaccgcg
     4201 acaacttcgg cgtcaatcac tatcattaca tcatcggcga caaggaattt gaaaaatttt
     4261 cttccaccgc ggtgcgcgcg gcggccgaat ccggcaatca ggaaaaactg aacaaattgg
     4321 tcaatccgca aatagccgct ctgatccttg ccaaattccg ccggaaaaat taaaatcatc
     4381 ataataactt taaaaatatg gaaacaacaa aacgggagac agccaaagat ttctttttgc
     4441 atttgctgtc ggcggcgacg ctctatatga gcgtgatcgg ttttatcgcg ctgtggtggc
     4501 aatacattaa ttttttattt cccgacaagt taaaccccat gggaatgtat gccggcagat
     4561 atgaaacatt actctgggcg acatcaatgc tggcggtgtc gtatccggtt tacgtgctgg
     4621 tgtcttggat cattggtcgg gggtttaaaa ccgatttggc cgggcgcgaa gtgaaaatcc
     4681 gtaaatggct gtggtacatc actctgttta tcgcggcgat gacgatgatc attgattcag
     4741 tcaccattgt cttcaatttt ttgaagggtg acttgacgat ccaatttttt ctcaaattgc
     4801 tggttatttt ggcaaccgcg gccgcggttt tcgggtatta tctttgggat ttaaaaaaac
     4861 gtgaaattgt ttcaaatctg cccaaaaaat tggcgtggat cgtcggtgcg gttgttcttg
     4921 ccagcattgg ctatggcttt gttttggcgg gatcgccggc aaaacagcgc gacatccgtt
     4981 ttgacgaacg ccgggtgaat gatttgcaaa acctgcaata tgcgattgtt gatttttggc
     5041 aatcgcgcca agaattgccg gcggagcttt ccgagcttga tgttttggga tatgccatgc
     5101 cggcggatcc ggcgacggga gaaagctatg gctacacggc gaccggagat ctgtcgtttg
     5161 agctgtgtgc ggaatttgcg gcggaatttg ccattgatga gggcaaggat cgttattctt
     5221 attataccgg gccgcaggga gtggcgactt tgcccaaagg atgggggcat aaagcgcaaa
     5281 gaacttgttt tgaaagcgtt attgacaaag atttttttga aagccaaatg aattacaaac
     5341 ccgacgcaat ttccgcacca atccgcgatt gaccgcggct gaaatgtgcg ctattgacaa
     5401 ttctgccgtt ttgtgattga atgaaaacat aattgaaatt ctcgttagtt tttgttcagg
     5461 cattattatc aatcctgtcc tcagtgagaa ccggcagtat cgcggctctt gaaaaactgg
     5521 cgaaatgaaa tattggccgg tttttcgttt ggcaagcgcc ctggaaaaca taacgggcca
     5581 aaaaggaaga aaagtatgca gacaaaagaa agaagcggcg gttttggcgg ttattttatg
     5641 aagtcaagat cgttttcggt cggcgatcgc cgaccgcgtt atcgcgctcc caaaaatttc
     5701 ggcgcgaaga aaagaggttt tggcggtatc aaaattgatc cggcaaaatt cgtgaataag
     5761 gccgttatgg ttgacggcaa cggaggcgaa cggatgaaca gtgccaaatt cgccgatttt
     5821 tccattgatg aaatcctcaa gcgaagaatt gcggccgtcg gctatcaaaa tgccacgccg
     5881 attcaagaat tggcgattcc gccgatcttg agcggtgcgg atgtggtggg cgttgccgat
     5941 accggcacgg gcaaaaccgc ggcattcttg gtgccgctga tcaacaaggt tttatcaaat
     6001 cggcgaatga ggattctgat tgtggcgccc acgcgcgaac tggcggcgca aaccgatgaa
     6061 gaattcaaaa agctggcgca aggaatgaaa atgttttcgg cgtgttgcgt gggcggggca
     6121 agcgttggcc gccaattggc gcagttgcgc tatttcaaca atttcattat cggcactccg
     6181 gggcggttga aagattttat tgaccgcaaa agaatcaatt tggcggaatt ttccgcaatt
     6241 gttttggatg aagccgaccg gatgatggat atggggttca tcgccgatat gcggtttttg
     6301 atggcgggca tgcccaagga gaggcaaacc ttgtttttct cggcgacgct gtcgccggaa
     6361 atcaaacgct tgatcggcga attcttgcgc gaaccggttt ttatttccat caaaacccgc
     6421 gatacttcaa aaaatgtgga gcaggatgtg gtgaaagttg ccgggcgcaa caaggttgac
     6481 cttttgcgcg aaatgcttgt tcgggaagaa ttcaaaaaag ttctgatttt cggccgcacg
     6541 aaattcggcg tggaaaaact ttgcaagact ttggaaaaat ccggcatcaa ggccgaatcc
     6601 atccatgggg acaagaaata ttcgcggcgc gtcaaggcgc tggaaacgtt taaatccgat
     6661 cgcgttcaag ttttggtggc caccgatgtc gccgcgcgcg gattggatat tgcccatgtg
     6721 acgcatgtga tcaattatga tctgccgcaa acttgggagg actatgtcca ccgcatcggc
     6781 agaaccggcc ggggcgccaa ttttggcaag gcgctcacct tcattgatta aatgccaaaa
     6841 aatcgccttt cgggcgattt tttgttaaaa taacggcata gtaaaaaaac tcgcaaaact
     6901 atggaaaatt tggagaatca aaaaattgaa acgctggatt attacgtgcg gcaaactttg
     6961 gataacttgc ccaaatggga atatccgaaa cttgaaatca aaaaaaactg ccgcaatgtt
     7021 tttgtgggat cgggcaatgc cgccaacgcc gggcggattt tggcgcggga attcggcggt
     7081 gcgggttttt cggtggttga ttatcaaagc tttttcaaaa attgcgatga tagcgaccgc
     7141 gatgtttata tcattaacgc ttcgggcggc aaggacggcg tgaagatggc gcaatggctt
     7201 acccaaaacg gccgacggcc gaaactcatc acttccaatc ccgaaccgcc ggcgggaaag
     7261 tttttgcgcg ttgaagatat tttcgcgttt ccggcgatca aggagccgcc gacctataat
     7321 gtgagcacct acgcggcgat gctctatggc attttaaaag aggacatctc cggcgccgac
     7381 gaaaggatca aaaatctgaa agttccggat ttgcgccgat ataaattcat tttctttttg
     7441 accgacgaca aatatgaacc tgccggccgc gtggcaaaaa gaaaggtggc cgaatctttg
     7501 gccggcttgg gcgcggacgc gggcggacac tccaacgcgg cgcatgggat gcttttgcaa
     7561 cccaatgccg atcgcttgat tgtttgcatc aattgcgatt acgaaggcga gggcgattgg
     7621 tggcgttttg acgccggttc atacattgag gcgattttgg ccgttcacta tattgtcggc
     7681 aaaaatcaga ccactgccga ttcgcaaaat attttgcgca attattttga gaacgccaaa
     7741 aaacaaggtt gggaattcaa caaagtttgg taagagagcc gttgtgaaaa ggtgcgtatg
     7801 gataaaaaag ttttgataat tttggttttc gcggcagtga tttttattgc cggcatcgtt
     7861 gcttcttttc cgaggaaaaa gggaagcgcg gttgaaatta acgcgccgcg agtttgcctg
     7921 aatggctttt gctatgaggt tgaagttgcc gacaatccga ctgatcgggc aagggggtta
     7981 atgtttcgtg aaagcttgga cgaaaacgag gggatgttgt ttgtttttga ccgggaagaa
     8041 aaacattcgt tctggatgaa gaataccttg atatccttgg atattgtctg gatgaactct
     8101 gccggcgaaa tcgttgaaat ttcggaaaat acgccgccgt gcgttgccga ttcctgtccg
     8161 agttatgttc ctgaggagcc ggcaaaatac gtgttggaga tttctgccgg ccagacgcaa
     8221 aaaaccagcg tgcggatcgg caatcgtgcc atcattgagt tttgattctc acttttccgg
     8281 aatgattttg gcgtcaaggg cgacgatgcc ggtttgatca accgccagcg gattgatgtc
     8341 aatttctttt atttggggaa aatccagcgg caagcgggaa aaacgcgcca tcacgccggc
     8401 aagctcttca atatcggcgc ctttgatgtt gcggtagcct ttgagcagtt tgaaaatttt
     8461 ggttttggcc atcaattctt tcgcgccggc ggcgtccagc ggcggcaggc cgatggcggt
     8521 gtcgtcaaaa atctccacgg cggtgccgcc cgcgccaaaa acgatcacgg ggccgaagag
     8581 aggatcaatc ttggcgccga cgatcaattc gtatttctta tcaaccattt tttccacggt
     8641 gatttcccga aaatctttcg cccgcaaatg tttttcggcg ttcttttcaa tttcatcaaa
     8701 cgcttttttg acttcggcgg cggtatcaag attcaaacgc acgccgccaa gttcggtttt
     8761 gtgcgccagg ccggattgca gtttcaacgc cacggggaag ccgatttttt tggcgatggt
     8821 cgccgcctgt ccggcggttt tcgccgggtg cgccggattg ccgggcaggc cgtaggcttg
     8881 caggattttt cgcgccgctt cgccgtcaat aatgctttgt tttttttctg ccgccgcggc
     8941 aaaaacttcc gccgcggtat ttttggcgga atcaaaatca aagcggaccc gggaaaaagc
     9001 tttttttgcc ctttttaaat ctttttgcca ttttgccagt cccaaaaagc aggcaatggc
     9061 tttttcgggc gtgcggtagg tcggaatgcc ggcgcgccgg aaaatttcca cgccttcggc
     9121 cacgcgggcc gcgcccataa aagaaataaa gaccggtttg gcggcaatgt cctcaatgcc
     9181 ggcgatccct tcggcgattt ttttgctttg agtgacggct tgcggcgaaa tgattgccat
     9241 aatgccgtca acatttttgt ctttcagaca ggcgctcacg gcggcgcggt aatgctccgg
     9301 cgcgccgtcg cccaaaacat caatcggatt ggcgcggctg gccgccggcg gcagtgtgct
     9361 ggccagcgtt ttgatggttt ttgtcgccaa ttttgacaat tcgccgccgt tttgcgccag
     9421 aaaatccgtt gaaataacgc ccgagccgcc ggcattggtg ataatcgcca aacggcggcc
     9481 ttgtggtttt ttgcaatggt tgagcgtgcg ggcgtaattg taaaattcac tgatggtggc
     9541 ggcgcggatc actccggcac gttcaaaaac cgctgaaaag accttgtcgt tgccggcgat
     9601 cgcgccggtg tgcgatgccg ccgccgccgc gcccgcggaa ttggcgccgg ctttcaggca
     9661 aacgattggt ttggtttggc aaaaatcgcg tgccgcggcg atgaagcgcg gaccgtcgtt
     9721 taaagattcc atatagagca gaatactgcc gacgttttca tcgtgggaaa aatattcaat
     9781 catttcggca aaatcaatat cggccgctga accaatggac acaaagtgcg aaaaaccgat
     9841 attgtcgcgc aaagaccaat ccaaaagagt gccgcaaagc gcgccgcttt gagagatgaa
     9901 ggcgatgctt cccggcaggg gtgattgcgg cgcaaaactg gcgttcagcc ccgcgcgagg
     9961 attggcaaat cccaggcaat tgggccccaa aatccgcatt cccgaacttc gggcaagttt
    10021 ttgtatcgca gaaaaatctt tttttccctg tttaccggat tctttgaacc ctgacgacac
    10081 aattactgcg ccgccggcgc cggcgcgggc acaatcgtcc aaaatcggca agaccgcggg
    10141 cgccggcgcg gcaatcaccg ccaaatcaac tttttcgccg atgtcgccga cgaaagggaa
    10201 cgctttttga ccctgcacgg cggcgttttt gttgttgact ggaaacactt tcccggcaaa
    10261 tgtgccgttt ttgatgttgg aaaaaatcgc gccgcccacc gaatggggac ggtcgctggc
    10321 gccgatgacg gcgatggttt ggggattgaa aaatccgtcc aatggttcgt tttgcataat
    10381 aagattttta tcgtttatcc ggccatacgg ttgatatttt tattttacaa catcctgccc
    10441 cacaaaccaa aaaagcgccg ggcgcttttg aaatttcaaa aaaagaggag ggcgatgcaa
    10501 aaaagatagt ggagtggcaa gcaacaacca aataggaggg agaaaccgtt gttgcttttc
    10561 ttgcatcgcc atgcgaggca tcggaagaaa gggattcccg agtctcaatt aaaagctata
    10621 cctttggctt gaaatgtcaa gagcattcag gccttaaaat attttgctca ggtttcaccg
    10681 cgccaagttg gggattgcgg gaaaatcaag gggtgatata attaaaacat ccggcgcgcg
    10741 ttggcgccgg aaaaatactt tgtttctcta tgggaggaga aaaaccgccg gaaaataaag
    10801 tttgccgttt gcgatcagtt tcaattaaaa tatcgccata aggattgccg gctttttgat
    10861 tgccggtttt gtttttcatc ggtcttgtat taaattggaa ggcgttaatg ctggaagt
//
LOCUS       DAOQWE010000014        10881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_6479_length_10881_cov_7.005542, whole genome shotgun sequence.
ACCESSION   DAOQWE010000014 DAOQWE010000000
VERSION     DAOQWE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6479_length_10881_cov_7.005542"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..2005)
                     /locus_tag="PKK37_01080"
     CDS             complement(53..2005)
                     /locus_tag="PKK37_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR81008.1"
                     /translation="MKFPSLAHWKQFFKILSPKERAAFFVCVIMAVGGGGVLTAALHQ
                     KYTLVVPADTGSYTEGVIGRPRFLNPVYAASHGPDQDIVELLFSGLLEYDSAGRIVPG
                     LADYNVSEDGKIYEFRLKEGAKWSDGREITSDDAIYTVKIIQDQAYKSPLRPQWIGVE
                     TEKVSETVFRFKLQNPYSSFPENCTLKIIPRHIWENTTADNFSLSPNNLNPVGSGRYK
                     FKRIVSDKSNVITAVELERNPLFSGPAPFLRQVNFAFFNDYEALAAAFKQGRIQGYAA
                     RESNGTVPAGATAYNYSIPRYFAVFFNPKKNEALADPAVRNALARAADKDEIIRAALA
                     GRGAAVDSPIAGVNPPPAAPKYDPEEAARLLDEAGYARGEDGVRVKTTSKKPAFQFTK
                     TLVKGSSATAEIQELQKCLAREVMPELEATGSFGDQTLKAVNLFQEKYRQDILDPQGL
                     KDPTGDVKLGTREKLNQVCFPSGDSAAKLEITIAVAAQEPFAAAAGIIKKQWEEIGVA
                     VNIREIPAADIERDAVKPRDYEALLFGQALGMIADPYPFWHSTQIADPGLNFAIYENK
                     DADKLLEEIRAAGDIAARAEKLAALQDLIIKDSPAVFLYNPEYRHIAAQSVKGMKSGP
                     IADPSRRFAGIAEWYTATKRIFNPGN"
     assembly_gap    2037..2136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2226..2447)
                     /gene="secG"
                     /locus_tag="PKK37_01085"
     CDS             complement(2226..2447)
                     /gene="secG"
                     /locus_tag="PKK37_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNR81009.1"
                     /translation="MADLISIVQIVISAGIVGLVLLQQRGGGGLGGAFGQAGGAYSTQ
                     RGVQKKIFNLTIVLAVVFIGLAIYNLFQR"
     gene            complement(2414..2773)
                     /locus_tag="PKK37_01090"
     CDS             complement(2414..2773)
                     /locus_tag="PKK37_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNR81010.1"
                     /translation="MLGKLIVYTAGSGFGLYLAVNLVPQISYSGGWPTLAIAGFCIAL
                     LNLTVAPILKILSIPLRVLTLNLFSLVIDMSMVWLANALIPELTIAGIGPLLAATVII
                     LLLNSILWLILSPSFRS"
     gene            2979..3272
                     /locus_tag="PKK37_01095"
     CDS             2979..3272
                     /locus_tag="PKK37_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNR81011.1"
                     /translation="MKISPCSDNVLLEPVKSEEKTKSGILLPDSASKERPEQGKVVAV
                     GPGKKNKQGQIIPLSVKVGQKVLFKKYSSNEIKVEEKEYLIIEENDILAVIED"
     gene            3412..5055
                     /gene="groL"
                     /locus_tag="PKK37_01100"
     CDS             3412..5055
                     /gene="groL"
                     /locus_tag="PKK37_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830243.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNR81012.1"
                     /translation="MAKEIVYSENARKKLKAGADKLANAVKVTLGPKGRNVVLDKSYG
                     APVITNDGVSIAKEIELEDRTENMGAQIVKEASEKTNDMAGDGTTTACVLAQALIAEG
                     LKNVTAGANPLAIKRGIDKGVDAVVASLKEMSKPISKKEEMAQVATISAESAELGNLI
                     AEVMTEVGKDGVVTVEESKNLDIRKEIVKGLQFDRGYSSAYMVTDAERMEAALEDPYI
                     LITEKKITSLPELLPILEKVMQTGKKELLIIADDIEGDALATLVVNKLRGIFNALAVK
                     APGFGDRKKEMLQDIAAVTGATVVSEQLGMKLENVTIKDLGSARRVVAGKEKTVIVEG
                     KGDKELIENRVKQIKNEIAKTESDFDKEKLQERLAKLAGGVAVIKVGAATEIEQKARQ
                     HKTEDALSATRAAVEEGIVPGGGVALLNAAKALDKLQVDGEEAIGINILKRALEEPIR
                     QIAANAGVEGAVVIEKIRQNNGTVGFNAAKMKYEDMLTAGVVDPTKVVRATIQNAASA
                     ASILLTTEAMVVEKPKKENPMPGGVPGMGGGMGGMDMGY"
     gene            5247..5480
                     /locus_tag="PKK37_01105"
     CDS             5247..5480
                     /locus_tag="PKK37_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR81013.1"
                     /translation="MKQTLHYNIILRAEPEGGFTVLVPALPGCVTYGKNLDEARRMAK
                     EAIELYIEDLRAHKDKIPNGCDYLISSLEVVSV"
     gene            5482..5706
                     /locus_tag="PKK37_01110"
     CDS             5482..5706
                     /locus_tag="PKK37_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR81014.1"
                     /translation="MTKLPAISPKKLINMLEKSGFAVSRVSGSHVILNRPMDKRRVVV
                     PCHAKDLPKGTLMSILHEAGIDKDRLKGLF"
     gene            5727..>6034
                     /locus_tag="PKK37_01115"
     CDS             5727..>6034
                     /locus_tag="PKK37_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81015.1"
                     /translation="MSGQFLHQNLARGRWFELSLVQQLGNIGSEVGRARRSCGNDEAK
                     FWNAAARALDLFYLTLSDKRWSKWRKKEICRACETFCDAVLGGQIYGGDLESLDRYFT
                     R"
     assembly_gap    6035..6134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6135..6449
                     /locus_tag="PKK37_01120"
     CDS             <6135..6449
                     /locus_tag="PKK37_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNR81016.1"
                     /translation="LRAEIKKDGCVKDPIIADKNTLVILDGHHRWNSLLSLGMAFCPC
                     CLVDYQSGEIGVVCWRENETITKKEVLSAGMTGKLLPQKTSRHFIPDRPLGLNVPLAD
                     LR"
     gene            6736..7059
                     /locus_tag="PKK37_01125"
     CDS             6736..7059
                     /locus_tag="PKK37_01125"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR81017.1"
                     /translation="MNKAAEKEIKRIVAIIAKKHRPEKIYLFGSFAWGKPRRDSDLDF
                     LVVKKGIKSMRQAALNIDRDFLDRKMAMDIIVYSPDFLRKRLSLEDPFAKKIIAQGKI
                     LYEKK"
     gene            7046..7438
                     /locus_tag="PKK37_01130"
                     /pseudo
     CDS             7046..7438
                     /locus_tag="PKK37_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
     gene            7503..8069
                     /locus_tag="PKK37_01135"
     CDS             7503..8069
                     /locus_tag="PKK37_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNR81018.1"
                     /translation="MEIATIVILAVVAALFVWLILNYNRLVAFKNRTKEAWADIDVQL
                     KRRYDLIPNLMETVKGYAAHEKQVLENVTAARSAAMDAKTPEEKIEKENALSQTLKSL
                     FAVAENYPDLKASANFLELQRELSDTENKIQAARRFYNGNVRDFNIAIESFPSNVVAA
                     IFGFKQMKLFEIAAEAEREAPKVSFEQK"
     assembly_gap    8380..8479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8600..9259)
                     /locus_tag="PKK37_01140"
     CDS             complement(8600..9259)
                     /locus_tag="PKK37_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNR81019.1"
                     /translation="MKLLLTSGGITNSSIANALFELTGKRPEEMTVAFIPTASNVEVG
                     DKDWLITDLINLKKQNFKSIEIADISAVNEKIWKPSLERADILFFEGGNTYHLMRWLN
                     KSGLADLLPELLKDKVYVGVSAGSMVTNPDLNLKLSQAIYEEDMSEAEELKGLNFVDF
                     YFLPHLNSEWFEKMRKENIEKAAQEINRTIYALDDNSALKIIDGKIEVVSQGKWFAIN
                     Q"
     gene            complement(9408..9695)
                     /locus_tag="PKK37_01145"
     CDS             complement(9408..9695)
                     /locus_tag="PKK37_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81020.1"
                     /translation="MDNTMVAAEYPLIGTNDFIKIAMGRPEPDPESKHNDRRCECKIS
                     APVDEKIFYVYGVDEIQCVWLGLRQIRREIAEFEKKTGAKCEYLYFQDPED"
     gene            complement(9740..10018)
                     /locus_tag="PKK37_01150"
     CDS             complement(9740..10018)
                     /locus_tag="PKK37_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNR81021.1"
                     /translation="MAKITSGKISKSDLRTIPGVGESIAGNLRNIGICSVADLKGKDP
                     QKLFEISNRFAGKAQDRCLLYVFREAVYFAENSNPDPERLKWWHWKDN"
     gene            complement(10053..10229)
                     /locus_tag="PKK37_01155"
     CDS             complement(10053..10229)
                     /locus_tag="PKK37_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81022.1"
                     /translation="MPDKKSNETAIRKLTRIGKKSISLTLPIDEVRNLGWREKQKVVV
                     KRVRGGLFIRDFKE"
     gene            10777..>10881
                     /locus_tag="PKK37_01160"
     CDS             10777..>10881
                     /locus_tag="PKK37_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81023.1"
                     /translation="MKKIRLEKGMSQGDICRSLGLDRAYISNVENGKQN"
BASE COUNT         2845 a         2343 c         2672 g         2721 t
ORIGIN      
        1 attataacaa atcttcaaat taaaagcaat tttttcgcat catttaggcg ccttagttgc
       61 cgggattgaa aattcttttg gtggcggtgt accattcggc gatgccggca aagcgtcgcg
      121 agggatcggc gatcgggccg gatttcatcc ctttgacgga ttgggcggcg atgtgccgat
      181 attccggatt gtacaaaaac accgccggag aatctttgat gatcagatct tgcagggccg
      241 ccagtttttc ggcgcgcgcg gcgatgtcgc cggcggcgcg gatttcttcc agcagtttgt
      301 cggcgtcttt gttctcgtag atcgcaaagt tcagtccggg atcggcaatc tgggttgaat
      361 gccagaaagg atacggatcg gcgatcattc ccagcgcttg cccgaacaac agcgcctcat
      421 aatcccgcgg cttgaccgcg tccctttcaa tgtcggcggc gggaatttcc cggatattga
      481 cggccactcc gatttcttcc cattgctttt tgatgattcc cgccgccgcc gcgaacggtt
      541 cttgagcggc aacggcgatt gtaatttcca gttttgcggc gctgtcgccg gaagggaagc
      601 agacctggtt cagtttttcg cgcgtgccga gtttgacgtc gccggtgggg tctttcaatc
      661 cctggggatc caaaatgtcc tgccggtatt tttcctggaa aagattaacg gctttcaaag
      721 tctggtcgcc gaaacttccg gtggcttcca attcgggcat tacttcgcgc gccagacatt
      781 tttgcaattc ctgtatttcg gcggttgccg aggagccctt gaccaaagtc ttcgtgaatt
      841 ggaacgccgg ttttttgctg gtggttttga cgcggacgcc gtcttcgccg cgcgcataac
      901 cggcctcgtc cagaagtcgc gccgcttctt cgggatcata ttttggcgcc gctggcggcg
      961 gattgacgcc cgcaatcggc gaatccacgg cggcgccgcg gccggccagc gccgcgcgga
     1021 taatttcatc cttgtccgcg gcgcgcgcca gcgcgttgcg gaccgccgga tccgccagcg
     1081 cctcgttctt tttgggattg aaaaaaacgg caaaataacg ggggatggaa taattatacg
     1141 ccgttgcgcc ggcgggcacg gtgccgttgc tttcgcgtgc cgcgtagcct tggatgcgtc
     1201 cctgcttgaa ggccgccgcc agcgcttcat aatcattgaa aaacgcgaaa ttcacttggc
     1261 gcaaaaacgg cgcgggtccc gaaaacaaag gattcctttc cagctcaacg gcggtgatga
     1321 cattgctttt gtccgaaacg attcttttga acttataacg tccggaaccg acgggattca
     1381 gattgttcgg cgacagggag aagttgtcgg cggtcgtgtt ttcccaaata tggcggggaa
     1441 tgatcttgag cgtgcaattt tcgggaaaag acgaataggg attttgcaat ttgaagcgga
     1501 aaaccgtttc ggaaactttt tcggtttcca cgccgatcca ttgcgggcgc aaggggcttt
     1561 tgtaggcctg atcctgaatg attttgacgg tgtagatcgc gtcatcgctg gtgatttccc
     1621 ggccgtcgga ccattttgcg ccttctttga ggcggaactc gtagattttt ccgtcttcgg
     1681 aaacattata gtcggccaat cccggaacga ttcgcccggc ggagtcatat tccagcagtc
     1741 ctgaaaagag cagttcaaca atgtcttgat cggggccgtg agaggcggca taaaccggat
     1801 tcaaaaatct cggccggccg atcacgcctt cggtgtaact gccggtatcg gcgggcacaa
     1861 ccagagtgta tttttgatgg agcgcggcgg tcaaaactcc gccgccgccg acggccatta
     1921 tcacgcaaac gaaaaacgcg gccctttctt tgggcgaaag aattttaaaa aattgtttcc
     1981 aatgcgccaa tgagggaaac ttcattaaaa ataatgcaaa atttaaaaat caaaatnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaat gcaaaattta aaaatggaaa
     2161 acacaaaacg ggacattttg catttcaaat tgtcattttg atttttgata tttacatttt
     2221 caattttacc tctggaagag gttgtatatc gccagaccga tgaagacgac cgccaaaacg
     2281 atcgtgaggt tgaaaatctt tttttgcacg ccccgctggg tggaataggc gccgccggcc
     2341 tgtccgaacg caccgccgag gccgccgccg ccccgctgct gcaaaagcac caggccgacg
     2401 atgcctgccg aaattacgat ctgaacgatg gagataagat cagccatagg attgaattta
     2461 acaataagat aataacggtg gccgccagaa gcgggccgat gccggcaatg gtcaattccg
     2521 ggatcagcgc gttggccagc caaaccattg acatatctat tactagacta aatagattta
     2581 aagtcaagac gcgcaggggg atggagagga tttttaaaat cggcgcgacc gtcaaattca
     2641 gcaaagcgat gcaaaaaccg gcgatcgcca atgtcggcca tccgcccgaa taactgattt
     2701 gcggaacaag attgacggcc agataaagcc cgaatccgct ccccgcggta tagacaatga
     2761 gttttcccag cataaccgca ttctaccaaa tcctccggat ttgacaaatt tttcgcaaaa
     2821 ggataaataa caattagcag tcggcggtcg cgactgataa ccaataaacc taaaaacaat
     2881 ttttaatttt taatttaaaa tttttaaaca atttttaatt tttaaaattt ggtcattgtt
     2941 taaaaattaa aaatttcaaa ttaaaaattt aagataatat gaaaatatct ccgtgttcgg
     3001 ataatgtttt gctggagccg gtcaaaagcg aagaaaaaac caaatccggc attcttttgc
     3061 ccgacagtgc gtccaaggag cgccccgagc aaggcaaggt ggtggccgtg ggtccgggca
     3121 aaaagaacaa acaaggccaa ataattcccc tgtccgtcaa ggtcgggcaa aaggtgctgt
     3181 tcaagaaata cagctccaac gaaatcaaag tcgaagaaaa agaatatctg atcattgaag
     3241 agaatgatat tctggcggtg attgaagact aaaattgaaa tttccaatgt caaaatccaa
     3301 atgtcaaaca aaatccaaaa tccaaatgcc aaaaagagaa atttattttt tggattttaa
     3361 attttgggct tgatttgaca tttatcattt gagctttgga tttaaaatac tgtggctaaa
     3421 gaaatcgttt attccgaaaa cgcccgcaaa aaactcaagg cgggcgccga caaactggcc
     3481 aatgccgtga aagtgacttt gggtcccaag gggcgcaatg tcgttttgga caagtcctat
     3541 ggcgcgccgg tgatcaccaa tgatggcgtt tccatcgcca aagagattga attggaggac
     3601 agaaccgaga atatgggggc gcaaattgtc aaagaggcgt ccgaaaaaac caacgatatg
     3661 gccggcgacg gcaccacgac cgcgtgtgtt ttggcgcagg cgctgattgc cgaggggttg
     3721 aaaaacgtaa ccgcgggcgc caatccgctg gcgatcaaac gcggtattga caagggcgtg
     3781 gatgcggtgg tggccagcct gaaagaaatg tccaagccga tttccaaaaa agaagagatg
     3841 gcgcaagtgg cgacgatctc ggccgaaagc gcggaattgg gcaatctgat cgccgaagtg
     3901 atgaccgaag tcggcaaaga cggcgtggtg accgtggaag aatccaaaaa tctggacatc
     3961 cgcaaggaaa tcgtcaaagg tttgcaattt gaccgcggct attcttcggc ctatatggtg
     4021 accgatgccg aaagaatgga ggcggcgctg gaagacccct acatcctgat caccgaaaag
     4081 aaaataactt ccctgcccga acttttgccg attttggaaa aagtgatgca gaccggcaaa
     4141 aaagaactgc tgattatcgc cgatgacatt gagggcgacg cgctggccac tttggtggtc
     4201 aacaaattgc gcggcatttt caacgcgctg gcggtgaagg ctccgggatt cggcgaccgc
     4261 aagaaggaga tgttgcaaga cattgccgcg gtaaccggcg cgacggtggt ttccgagcaa
     4321 ttggggatga aattggaaaa tgtcacgatc aaggatttgg gttcggcgcg gcgcgtggtg
     4381 gcgggtaaag agaaaactgt catcgtggag ggcaagggcg acaaggagct gattgaaaac
     4441 cgcgtcaaac aaatcaagaa cgaaattgcc aagaccgagt cggattttga caaagaaaaa
     4501 ctgcaagagc ggctggcgaa attggccggc ggcgtggcgg tgattaaagt cggcgcggcc
     4561 accgagattg agcaaaaagc ccgccaacac aagaccgaag acgcgctgtc ggcgactcgc
     4621 gcggcggtgg aggagggcat tgttcccggc ggcggcgtgg cgctgttgaa tgccgccaaa
     4681 gcgctggaca agcttcaggt tgacggcgaa gaagccatcg gcataaacat tttgaaacgc
     4741 gcgctggaag agccgatccg ccagattgcg gccaatgccg gcgtggaagg cgcggtggtg
     4801 attgaaaaaa ttcgccagaa caacggcacc gtgggtttca acgccgcgaa aatgaagtat
     4861 gaagatatgc tgacggcggg cgtggtggac cccaccaaag tggtgcgcgc cacgatccag
     4921 aacgcggcct ccgcggcgtc catcttgctg accaccgaag ccatggtggt ggagaaacct
     4981 aaaaaggaga atccgatgcc cggcggtgtg cccggaatgg gcggcggtat ggggggaatg
     5041 gatatgggct attagaatca ttaatcatta ataattaatc attaaagatt gcgcaagcgc
     5101 tcccgcaagg gggcgtttgc gtttgggcgg ccaaaaaata aactacgccg gggcagagcc
     5161 cacaaggtat tctggtggat gaataaaaat aaaataggga atttgtcaag gttgaagggg
     5221 tgggcgcaag gtgatatgtt gaggatatga aacaaacatt gcattacaat attattttga
     5281 gagcggaacc cgaagggggc tttacggtgc tggtgccggc attgcccgga tgcgttacct
     5341 atggcaaaaa tttggacgaa gcgcggcgaa tggcaaaaga ggcgattgaa ttgtacatag
     5401 aagatttaag ggcgcataaa gataaaattc ccaatggttg cgattattta atctcgtcat
     5461 tggaagtggt ttccgtttaa tatgaccaaa ttgccggcaa tttcgccgaa aaaactgatt
     5521 aacatgctgg aaaaatccgg atttgcggtg agccgcgttt ccggcagtca cgtaatttta
     5581 aatcgtccga tggataaaag aagagttgtc gttccttgcc acgcaaaaga tttgcccaaa
     5641 ggaaccttga tgtcaatttt gcacgaagcg ggaattgaca aagatcggtt aaaaggattg
     5701 ttttaaaggc agggtagaat aaaataatga gcggtcaatt tttgcatcaa aatttggcgc
     5761 ggggtaggtg gtttgagctg tcgcttgtcc agcaattggg gaatatcggc agcgaggtgg
     5821 ggcgcgcgcg gcggtcttgc ggcaatgatg aagcgaaatt ttggaacgcc gcggcgcgcg
     5881 cgctggattt gttttatctg actttatccg acaagcgctg gagcaagtgg cgcaaaaaag
     5941 aaatttgccg cgcttgcgaa actttttgcg acgcggtttt gggcggacaa atttatggcg
     6001 gcgacttgga atcgcttgac cgctatttta cccgnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnttgcgc gcggaaatca aaaaagacgg atgcgttaag gatccgataa
     6181 tcgcggacaa aaacacattg gtgattttgg acggccacca tcgctggaat tctcttttgt
     6241 cgctgggcat ggcgttttgc ccgtgctgtc tggtggatta ccaaagcggc gagatcggcg
     6301 ttgtttgctg gcgcgagaat gaaacaatca ccaaaaaaga agtgctgtct gcggggatga
     6361 ccggcaaact tctgccgcaa aagacctccc gccatttcat tcccgaccgc cctctcggcc
     6421 tgaatgtccc gctggcggat ttgcgataaa tatatgaacc agtgcggtac ttaggaggct
     6481 ttcacaaata atatgcgcac catacccgca tctttgcccc atatttcaaa tttcctaggt
     6541 cgcggtattt tcggtgtggc gattgccaca cctcaaacat cgttcggtcg gaaatttgaa
     6601 atctggaaac aaatctacgg gtaatatatg catattattt gcgaaagcct ccttagaatc
     6661 atattttata atttgtcaag atagccaaga gagcggagat ggggtataat cacaaaatca
     6721 tatagcgtaa attttatgaa taaggcggcg gaaaaagaaa taaagcggat tgtcgcgatt
     6781 atcgcaaaaa agcaccggcc cgagaaaatt tatcttttcg gttcctttgc gtggggcaag
     6841 ccgcgccgcg acagcgacct tgattttttg gtggtgaaaa aaggaattaa atcaatgcgc
     6901 caggccgcgc ttaatataga ccgcgatttt cttgatcgca aaatggcgat ggatatcatc
     6961 gtctattctc ctgatttctt gagaaagaga ttatccttgg aagatccctt tgcgaaaaaa
     7021 attattgcgc aagggaaaat tctttatgaa aaaaaataat ttaattgtag ctcgcgaatg
     7081 gttcgcgcat gccgatgaag acatattgtc atgcgaggcc atcataaaag aaggaggcgg
     7141 cccaagcact ttgtgttttt tgtcgcattt gattgcggaa aaatatttca aaggactgtt
     7201 gatttcttct ggcaaggact tggtaaaaat tcatgatctt gtgaagttgg tgaatttgct
     7261 caaaaaagat tttcccgcga tttgctaatt gatggatgaa ttggcacttt tgaataaata
     7321 ttgcattgat gcgcgttatc ccggagcgtc ggagggattt agctggaaag aagctaaaaa
     7381 tgcgtttgag attgccaaaa aggtcaaaaa aattgttatg aacgcaataa ggctttaaaa
     7441 atttcccaaa agtagtagta tcaatagtag tattaaatga agcatagaat aacaaaaaaa
     7501 atatggaaat tgccacaatc gttattttag ccgttgtcgc cgcgcttttt gtctggctga
     7561 ttctcaacta taatcgcctg gtggcgttca aaaaccgcac caaagaggcg tgggccgaca
     7621 ttgacgtgca gttgaagcgc cggtatgacc tgattcccaa tctgatggaa acggtcaagg
     7681 gttacgcggc gcatgaaaag caggtattgg aaaacgtcac cgccgcgcgt tccgcggcga
     7741 tggacgccaa aacacccgag gaaaaaattg aaaaagaaaa cgcactgtcg caaactttga
     7801 aaagtttgtt tgccgtggcg gaaaactacc ccgatttgaa agcgtcggcg aattttttgg
     7861 aacttcagcg cgaattgtcc gacaccgaaa acaaaattca agcggcgcgc cggttttaca
     7921 acggcaatgt gcgcgacttt aacatcgcca ttgaaagctt tccgtccaat gtcgtggccg
     7981 caatcttcgg cttcaagcag atgaagttgt ttgaaattgc cgccgaagcc gaacgcgaag
     8041 ctcccaaagt gagctttgaa caaaagtaga attacacaaa tccgaatatt gaattttcga
     8101 atatcgaaac aaatttaagt tttcaaattt ttagatgaac aaaacaaatt attgtatctt
     8161 gttttgaaat ttagattttg aacattcgat attgtttcgg atttcgtgct tcgaatttcg
     8221 gattttattg tttgtggcta ccatctatac tcatgccgat gaaaatattc aaaaaacctg
     8281 gttgttgcta accttctttt ttgtgtttct tgtcgggctg ggatggctgt tgtcgcaggc
     8341 gctgggggat tcgtctttcc tttacatcgc ggtggcgatn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnc gcatccgcgc cctgaccgaa atgtcaatct agcccaaaat
     8521 taaaaattac ataggtgtca cctatgtaat tttttgtcat aacagcttat atattgccaa
     8581 ggcagggctt cggcggattc tattggttga tggcaaacca ttttccttgg ctgaccactt
     8641 caattttgcc gtcaatgatt tttagcgcgg aattatcatc aagagcgtaa atcgtcctgt
     8701 ttatttcttg cgcggctttt tctatgtttt ctttcctcat tttttcaaac cattccgagt
     8761 ttagatgcgg caaaaaataa aaatctacaa aattcaaacc tttcaattct tcggcttccg
     8821 acatatcttc ttcgtatatc gcttgcgaca attttaaatt caagtccgga ttggtcacca
     8881 tgcttccggc gctgactccg acatatactt tatctttcaa taactccggc aataaatcgg
     8941 ccaagccgga tttgtttagc catctcatca gatgataggt atttcctccc tcaaaaaata
     9001 aaatatcggc tctttccaag ctcggcttcc atattttttc attaaccgct gaaatatcgg
     9061 cgatttcaat tgatttaaaa ttttgttttt ttaaatttat cagatcggta ataagccaat
     9121 ccttgtctcc gacttcaaca ttgctcgccg tcgggatgaa ggcaacagtc atttcctccg
     9181 gccttttgcc ggttaattca aataacgcat tggcgattga gctgttcgta attccgccgg
     9241 atgtgagtaa tagtttcata ttggttgatt tttataacga tgattgtgtg atttttgcaa
     9301 tagctcttgc tgtgctggaa gcggggaggt cattttcatt tttttgattt tctaaaaaac
     9361 aaaaatagca ccgcggattt caagcggtgg aaaattcatt taatcctcta atcctccgga
     9421 tcttggaagt aaagatattc gcattttgcc ccggtctttt tctcaaattc ggcgatttcc
     9481 cttcttattt gcctcaaccc cagccaaacg cattgaattt catcaacgcc ataaacataa
     9541 aaaattttct catcgacggg cgcggaaatt ttgcattcgc aacgccggtc attgtgtttt
     9601 gattccgggt ccggctcggg tcggcccatc gcgattttaa taaaatcatt ggtaccgatg
     9661 agcggatatt cggcggctac cattgtgttg tccatagttt tttcttgttc ataaattata
     9721 agttattaat gattaatgat taattgtctt tccaatgcca ccacttgagc ctttccggat
     9781 cgggattgga attttcggca aaatagactg cctcgcggaa aacataaagc aagcaccggt
     9841 cctgggcctt gccggcaaag cggttggata tttcaaacag tttttgggga tccttgcctt
     9901 tcaaatccgc caccgaacaa atcccgatat tgcgcaaatt accggcgatg ctttcgccca
     9961 ctccgggaat cgtccgcaga tcgcttttcg aaatttttcc acttgttatc ttagccatag
    10021 cttttgcgtt tctgcgggca aatgcggccc ctctattctt taaagtcgcg gatgaacagg
    10081 ccgccgcgca cgcgcttcac gaccactttt tgtttttcgc gccagcccaa attcctaacc
    10141 tcatcaatcg gcagggtcag gctgattgat tttttgccga tgcgggttaa tttgcggatg
    10201 gcggtttcgt ttgatttttt gtccggcata attgaaaaag tcggtaattg attacttaat
    10261 tgattactta catcaatatt aatccaaagc cgcaagtctt gcaaaaaccg cccccgacgg
    10321 gacggaattc gcggatgcga ttttcaattc ggaaagcatt ttccgctgaa tgtaaaaatt
    10381 ggcggtgttt tttggacgaa gttcgaacat tgtatgagca aaatccgcag gattttgacg
    10441 actgacgcac gcgcggagcg cgtggtggct ctttcccaaa tcgtacgctc ggattgcgtg
    10501 gtgaagcgaa cgtggcgcgc ctcgcgctcc ctccggtcgc gaaccaaaac caaaaatttc
    10561 cttaccaatc catttccggc ggggggtgtg gggggagccg cttaaaaaat gaaaggaaat
    10621 tttatggttt tgtttccgcc gcttcacaat ccaattattc acacttgtat tataacttat
    10681 tattgagcca aaacacaaca taatgttata ctgacttgtg ttgataagtt ttgataaaat
    10741 atgagtaaat ccacagccca aaagtttggc gaaaacatga agaaaattcg ccttgaaaaa
    10801 ggcatgtcgc aaggcgacat ctgccgatcg ttaggtttag acagggcgta tatcagcaat
    10861 gttgagaacg gcaagcaaaa t
//
LOCUS       DAOQWE010000015        10621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_6700_length_10621_cov_7.251562, whole genome shotgun sequence.
ACCESSION   DAOQWE010000015 DAOQWE010000000
VERSION     DAOQWE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6700_length_10621_cov_7.251562"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..2284)
                     /locus_tag="PKK37_01165"
     CDS             complement(2..2284)
                     /locus_tag="PKK37_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNR81024.1"
                     /translation="MKKNHRRRASGRVKKNFKIPELFHFPGRTGRWAAGILFAVAGLV
                     LALGFFGLAGMIGNWFAAGLRGALGGAAFLLPVILFAAGGACAISRQARFAASLSLAS
                     LLFLGGAAGFLANLDYTGRAGGAIGYYLGLPAFNLFGSWVAGAFFAALFAAGALVFWW
                     LLGRPAPDFSKFRLPAPRAKETKKTALALKKKAVPEKETLTAKKPFAAAIAIPKFSMI
                     NVRGAAPKNDKGQSAAAKKSAAARGALMEKVQYKTPPLDLLDCRDSAPLAGDVKQNSA
                     IIKKTLENFGIPVEMSPEIIVGPSVTQYALKPAEGIKLSKITTLSNDLSLSLASSSIR
                     IEAPIPGKSLVGVEVPNKSRAGVGLRELLDTDQFAQSPANLLITLGKDILGSPIYSDI
                     ARMPHLLVAGATGTGKTIFLNSVLTSLLYRNPPETLRLILVDPKRVEMRGYNKLPHLL
                     TPVIFDVNKTIAALKWAIAEMDRRFDILGEKESRNIASYNEKCAKDGDEPLPYIVFVI
                     DEMADLMATKGKEIEAGIVRLAQMARAVGIHLIVATQRPSVEVITGLIKANITCRVAF
                     QVASQIDSRTILDISGAEKLLGAGDLLFMSSEYPKPKRIQGPYISEKESKKVLEWIKE
                     NNSPPEFKIEDGVLESLMMGEPGREYNGGGSMFGVPEGAGEGFGDDPLYEQAKAVVVQ
                     ARKASASFLQRRLRVGYARAARLIDLLEERGVVGQGDGAKPRDVLVSVIDSPGDVPGG
                     MSGDVNEVEGGQGDDGWQKV"
     assembly_gap    2301..2400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2403..6158)
                     /gene="rpoC"
                     /locus_tag="PKK37_01170"
     CDS             complement(2403..6158)
                     /gene="rpoC"
                     /locus_tag="PKK37_01170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR81025.1"
                     /translation="MRVEDLESIRIKLASPEEVVSWSHGEITKPETINYRTQRPEKDG
                     LFCERIFGPERDYECYCGKYKRVRYKGIVCDRCGVEVTKSSVRRERMGHIKLACPVSH
                     IWFLRGVPSRIGQVLNLPMLALEKVIYFAAYIIISADNAEKEKISAEIDAEFNEKTKT
                     QKRLFESQNPNAKKSDDKEAQKALQAILDELRDARDRAKSELASIEFMRVISEAEYRN
                     LSLKYGQCFSAGTGAETVRKLCEKVDLAELVKNLKVQLETATPEEKRKIYIRLKLLQG
                     MHQAGIRPEWMLITALPVLPADLRPIVQLDGGRYASSDLNDLYRRVINRNNRLKYLQE
                     IAAPEVIVRNEKRMLQEAVDSLIDNGMRKGTTTQATTGGRRLLKSLADMLKGKQGRFR
                     QNLLGKRVDYSGRSVIVVGPHLRLNQVGIPKKMALELFKPFVIHRVLEKEMAFNVRGA
                     SRLIEAETDEIWAILEEVVKDKLVLINRAPTLHRLSIQAFYPVLIEGEALQIHPMVCK
                     AFNADFDGDQMAVHLPLSALAQKEARERMLSSLNLLKPAKGTPVVTVYQDMVLGCYWL
                     TAEVAGAKGEGKVFAGPDEAVMVYEFGDLDMRAKIKVRGVKPSAPGFTETTVGRIIFN
                     RTLPEDFAFVNQKVKVKDLENIAEGVIRHYDVQTVEDTLDRIKELGFEYSTLSGISWG
                     MDDLHVPAEKAGIIKEAEKEVEKTEDYYQKGLLSKDEKKNKIIEIWSKTKATIENLVP
                     KTLSEESSVYQMIDAGARGSWSQPVQMAGMKGLVVDPSGDIIELPVKNSYKEGLDVLE
                     YFNSTHGARKGTVDTALRTSTAGYLTRRLVDVAHEAIISQEDCGDTAGMEIYLKESEE
                     IGQNFLYKIVGHVCAETVKSDKETIVKKGEAIDWETGRRLIAAGIEKIKIRTPLSCKS
                     ERGLCQKCYGWDLGNNQMVRLGEAVGIIAAQSIGEPGTQLTMRTFHTGGVASGTDITS
                     GLPRVEEIFEARIPGGKAEICPADGTVLEVISEKGQNKIIRIKIKSKDAPKDGKKETK
                     KRKKAAEDKKEDVPARAANFQTIEFSAPANVNVLVRAGEEIVKGQPLWEGSLDLKELY
                     KLVGRDAVQRYILREVQHIYSSQGATIHDKHVAVIIRQMFSRVKIKDPGDSLFTPKEV
                     VELKQVLEENKRLIEQKLRPVDFVPVLLGVSKVALTTGSWLSSASFQETSRVLIKASL
                     ERLEDRLDGLKENVIIGKLIPAGTGFGAGAAVTEEALPAEDGDVPKN"
     gene            complement(6305..9496)
                     /locus_tag="PKK37_01175"
     CDS             complement(6305..9496)
                     /locus_tag="PKK37_01175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNR81026.1"
                     /translation="MALKTKNFGKAKVSLPLPYLLVLQKESWDMFWGNDLRELFTEIS
                     PIRDYTGKEMELWIDDFKLDKPKHKNDLDARLNNDSYESPVRVKTRLVNLKTKETKTQ
                     EIFLCDFPLMTERGTFVINGVERVCISQLIRSAGVFFSAQNIGGKNFFGTKIIPNRGS
                     WLEFETAHTGFIGVKIDRKRRVPATTLLRAFGIDNDNAIKELFADVNNGEVDFIEETI
                     KKDPTHNQAEALVEIYQRLRPGDLTTADTAKELIWNMFFNFERYDLSKVGRWKMWKRL
                     PQLKPKDKKNDVVEMKDRVMTVDDIVAVIREIIRLNNDPAAKPDEIDHLGNRRVRTLN
                     ELLVNRLRLGFMRMERIIKDRMSTLDVLELSPLQLINPRPVMAVIKEFFSSSQLSQFM
                     DNENPLSELEHKRRLAATGPGGLTRERAGFEVRDVQPSHYGRICPIQTPEGANVGLVG
                     YLASYTKINDFGFLETPYFEVKKNKVTDKLVYMTADEEEKYNIATAIAPLDENNNIIP
                     KKLEARIKGEPGICAREEIDYIDVSPQQILSVGTSLIPFLQNDDANRALMGSNMQRQS
                     TPLLLPEVPLVMTGSEERIARDSGHILPAEDAGVITEVDGRHIAVKYDDKELGEKTYY
                     LRTFLRTNQYTCFHQKAAVKKGQKVKKGDALCDGGGIADGHLALGHNVLVAFTSWRGG
                     NFEDAILLSERLVADDKFTTIHMENFACDVRETKLGPETTTYDIPNVSEEKLKDLDEE
                     GVVRVGAEIKPNDILVGKISPKGEQELTAEERLLRSIFGEKAKDVKDTSLVMEHGKRG
                     RVVGVKIFSREQGHKLDPGVIKRIEIEVAELRKVRAGDKLAGRHGNKGVISRILPVEE
                     MPFLEDGTPVDIVLNPMSVASRMNLGQILETHFGYAAHKLGYHAVTPSLSGATEADIK
                     AELKKAGLSEDGKEKLFDGRSGLPFPGKITVGYIYMMKLVHMVEDKIHTRSIGPYSLI
                     TQQPLGGKAQFGGQRFGEMEVWALEGYGAAHTLQEMLTIKSDDIAGRAATYETILRGE
                     DIRHPNIPASFNLLVSELKSLGLSVEVKERNNQ"
     gene            complement(9966..10097)
                     /locus_tag="PKK37_01180"
     CDS             complement(9966..10097)
                     /locus_tag="PKK37_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81027.1"
                     /translation="MPDKLPADQLWQEWVKKAEDAYAAAQRIKEFVLSEIAKKPAGD"
     gene            complement(10087..10410)
                     /locus_tag="PKK37_01185"
     CDS             complement(10087..10410)
                     /locus_tag="PKK37_01185"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR81028.1"
                     /translation="MVKEETKRKIQAIADKIAREYKPEKIILFGSYAWGHPHEDSDLD
                     LFIIKKTDDPMLKRMEDVDKLFFPREFAMDLLVYTPEQVDKRKKMQDPFVIDIIDKGK
                     LLYAR"
BASE COUNT         2211 a         3101 c         2596 g         2613 t
ORIGIN      
        1 ctcaaacttt ctgccagccg tcgtcgcctt gcccgccctc aacctcgttg acatcgcccg
       61 acatcccgcc gggaacatcc cccggagaat caatcaccga caccagcacg tcgcgcggtt
      121 tggcgccgtc gccctgcccc accacgccgc gctcttccag caaatcaatc agccgcgccg
      181 cgcgggcata gcccacgcgc aagcgccgct gtaaaaatga cgccgacgct ttgcgcgcct
      241 gcaccaccac cgccttggct tgctcataca acggatcatc gccgaatccc tcgccggcgc
      301 cctcgggcac cccgaacata ctgccgccgc cgttatactc tcggccgggt tcgcccatca
      361 tcaggctttc cagcactccg tcttcaattt tgaattccgg cggggaattg ttttccttga
      421 tccattccaa aacttttttg ctttcctttt cggaaatata aggcccctgg atgcgtttgg
      481 gtttgggata ttccgacgac ataaacagca gatcgccggc gcccagcaat ttttccgccc
      541 ccgaaatatc caaaatcgtg cgcgaatcaa tctgcgacgc cacctgaaac gccacccggc
      601 aggtaatgtt ggccttgatc aagccggtga tcacttccac gctcggacgc tgggtcgcca
      661 cgatcagatg aatgcccacc gcccgcgcca tctgcgccag gcgcacgatg cccgcctcaa
      721 tttccttgcc cttggtcgcc atcagatccg ccatttcgtc aataacgaaa acgatatagg
      781 gcaaaggttc gtcgccgtcc ttggcgcatt tctcgttgta actggcaatg tttcttgatt
      841 ctttttcgcc caaaatgtca aaccggcgat ccatttccgc gatcgcccat ttcagcgcgg
      901 cgatcgtttt gttgacatca aaaatcaccg gcgtcagcaa gtgcggcagt ttattgtagc
      961 cgcgcatctc cacccgcttg ggatccacca aaatcaagcg tagcgtttcc ggcggattgc
     1021 gatataaaag agaagtgagc acgctgttca agaatatcgt tttacccgtg ccggtcgcgc
     1081 ccgccaccag caaatgcggc attcgggcga tgtcggaata aatgggactg cccagaatgt
     1141 ccttgcccaa agtgatcagc aaattcgccg gcgattgcgc gaattggtcg gtatccaaaa
     1201 gttcgcgcaa ccccacgccg gcgcggctct tgttgggcac ctccacgccc accagcgatt
     1261 ttcccggaat cggcgcttca atgcggatgg acgaactggc cagcgacaac gaaagatcgt
     1321 tggacagcgt cgtaatcttg gaaagcttga tgccctcggc gggcttgagc gcgtattgcg
     1381 tcaccgacgg ccccacgata atttccggcg acatttccac cggaatgccg aaattttcca
     1441 aagttttttt gatgatcgcc gaattctgct tgacgtcgcc ggccagcggc gccgaatcgc
     1501 ggcaatccaa aagatccaag ggcggcgttt tatattgcac tttctccatc agcgcgccgc
     1561 gcgccgccgc ggattttttg gcggcggccg actggccctt atcgtttttc ggcgccgcgc
     1621 cccggacgtt aatcattgaa aatttgggaa tggcaatcgc cgccgcgaac ggttttttgg
     1681 cggtcaaagt ttctttttcg ggcactgctt tctttttgag cgccagggcc gtttttttgg
     1741 tttctttggc gcgcggcgcc ggcaggcgga atttggaaaa atccggcgcc ggccgcccca
     1801 aaagccacca aaaaaccagc gcgccggcgg cgaagagcgc cgcgaaaaac gcgcccgcaa
     1861 cccaggaacc gaacagattg aacgccggca aacccaaata atagccgatg gcgcccccgg
     1921 cgcggccggt gtaatcaagg tttgccaaaa acccggccgc gccgcccagg aacaagagcg
     1981 aggccagcga cagcgatgcg gcaaatctcg cctgccggga aattgcgcag gcgccgccgg
     2041 cggcgaaaag aatcaccggc agtaaaaacg ccgcgccgcc cagcgcgccg cgcagtccgg
     2101 cggcgaacca gttgccgatc atccccgcca aaccaaaaaa cccgagcgcc aaaaccagcc
     2161 ccgccacggc aaacaaaatg ccggcggccc agcgtccggt gcgcccggga aaatggaaca
     2221 attcgggaat tttgaaattt tttttgaccc ggccgctcgc gcggcggcgg tggttttttt
     2281 tcatacgcag aattatgaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 attcagttct tcggaacatc gccgtcttcg gcgggcagtg cctcttccgt tactgccgcg
     2461 ccggcgccga atcccgtgcc cgcgggaatc agtttgccga tgattacgtt ttccttcaat
     2521 ccgtcaagac gatcttccag ccgctccaaa ctggccttga tcagcacccg cgaagtttcc
     2581 tgaaaggatg ccgacgaaag ccatgatccg gttgtgagcg ccactttgga aacgcccagc
     2641 aggaccggca caaagtccac cggccgcagt ttttgctcaa tcagccgctt gttctcttcc
     2701 agcacttgtt ttaattccac cacctctttg ggagtgaaca agctgtcgcc gggatctttg
     2761 atcttcacac gggaaaacat ctggcggatg atcaccgcca cgtgtttgtc gtggatcgtc
     2821 gccccctgag aactgtaaat atgctggact tcccgaagaa tgtagcgttg caccgcgtcg
     2881 cggcccacca gcttgtagag ctccttcaaa tccaaactgc cctcccagag cggctggcct
     2941 ttgacgatct cctcgccggc gcgcaccaga acattaacgt tggccggcgc cgaaaattca
     3001 atcgtctgga agtttgcggc gcgggccgga acatcttcct ttttgtcctc ggcggctttt
     3061 ttccgctttt tggtttcctt cttgccgtcc ttgggcgcgt ctttggattt gatcttgatc
     3121 ctgataatct tattttggcc cttttcggaa atgacttcaa gcaccgtgcc gtccgccggg
     3181 caaatctcgg ctttcccgcc gggaatccgc gcttcaaaaa tttcttccac gcgcggcaaa
     3241 ccggaagtga tgtccgtgcc cgaagccacg ccgccggtat ggaaggtcct cattgtcaac
     3301 tgggtgcccg gctcgccgat ggattgcgcc gcgatgatgc ccaccgcttc gcccagccgc
     3361 accatctggt tgttgcccag atcccagccg tagcatttct ggcacaatcc tctttcggat
     3421 ttgcacgaca gcggcgtcct gattttgatt ttttcaatgc ccgcggcgat cagccggcgg
     3481 cccgtttccc aatcaatcgc ctcccccttc ttgacgatcg tttccttgtc ggacttgacg
     3541 gtttcggcgc aaacatggcc gacgatcttg tacaggaaat tctgcccgat ttcttcggat
     3601 tctttgaggt aaatctccat tccggcggtg tcgccgcaat cctcctggct gataatcgct
     3661 tcgtgggcga catccaccag ccgccgcgtc agatagccgg cggtggaagt tctcagggcc
     3721 gtatccaccg tgcctttccg cgcgccgtgg gtggaattga agtattccag cacgtccagc
     3781 ccttccttgt atgaattttt caccggcagt tcaatgatgt ctccggacgg atccaccacc
     3841 aatcctttca tccccgccat ttgcaccggc tgggaccacg aaccgcgcgc gccggcgtca
     3901 atcatctggt agaccgagct ttcctccgac agggttttgg gcaccagatt ctcaatcgtc
     3961 gccttggttt tggaccaaat ctcaatgatc ttgtttttct tttcgtcttt tgacaaaagc
     4021 cctttttgat aataatcttc ggttttttcc acctcttttt ccgcctcttt gatgatgccg
     4081 gccttttccg ccggcacgtg gagatcgtcc attccccatg aaattcccga caatgtggaa
     4141 tattcaaaac cgagttcctt gatgcggtcc aaagtgtctt ccaccgtttg cacgtcgtag
     4201 tggcggatca cgccttcggc gatgttttcc aaatctttga ctttcacttt ttggttgacg
     4261 aacgcgaaat cttccggcag agtgcggttg aaaataatcc ggccgaccgt ggtttcggta
     4321 aaaccgggcg ccgacggctt gacgccgcgc actttgatct tggcgcgcat atccaaatcc
     4381 ccgaattcat agaccatcac cgcctcgtcg gggccggcaa aaaccttgcc ctcgcctttc
     4441 gcgccggcaa cctcggcggt gagccaataa cagcccagca ccatatcctg ataaaccgtc
     4501 accaccggcg tgcccttggc gggcttcaac aaattcaagc tggaaagcat tctttcccgc
     4561 gcttcctttt gcgccagcgc cgacagcggc agatggaccg ccatctggtc gccgtcaaag
     4621 tcggcgttga acgccttgca gaccatcgga tggatttgca gggcctcgcc ttcaatcaag
     4681 accggataga acgcctgaat gctcaagcgg tgcagggtgg gagcgcggtt aatcagcacc
     4741 aatttgtctt tcaccacctc ttccaaaatt gcccaaattt cgtcggtttc cgcctcaatc
     4801 aagcgcgacg cgccgcggac gttgaatgcc atctcttttt ccaaaacgcg gtgaatcacg
     4861 aacggcttga acaattccag cgccattttc ttgggaatcc ccacttgatt aagccgcaaa
     4921 tgcgggccga cgacaatcac cgagcgtccc gaataatcca cgcgcttgcc caaaagattc
     4981 tggcggaacc gcccttgctt gcccttcagc atatcggcca aagatttcaa cagccgccgg
     5041 ccgccagtgg tcgcctgcgt ggtggtgccc tttctcattc cgttgtcaat cagcgaatcc
     5101 accgcttcct gcaacattct tttttcattg cgcacgatca cttccggcgc ggcaatctcc
     5161 tgcagatatt tgagccggtt gttgcggttg atcacgcggc gatataaatc attcaaatcg
     5221 gaactggcat accggccgcc gtccagctgg acgatcggac gcaaatccgc gggcaaaacc
     5281 ggcagggcgg tgatcagcat ccactccggg cggatgccgg cctggtgcat tccctgcaaa
     5341 agcttcaggc ggatgtagat ttttcttttt tcctcgggag tcgcggtttc cagctggact
     5401 ttcaggttct tgaccaactc ggccagatca actttttcac acagcttgcg gacggtttcc
     5461 gcgccggtgc cggccgaaaa acattgcccg tatttgagcg acaaattgcg gtattcggct
     5521 tccgaaatca cccgcataaa ttcaatggac gccaattccg atttggcgcg gtcgcgcgcg
     5581 tcgcgcagtt cgtcaaggat cgcctgcaag gccttttgcg cttctttatc atcgcttttt
     5641 ttcgcgttcg gattttggga ttcaaaaagc cgtttttgcg ttttggtctt ttcgttgaat
     5701 tccgcgtcaa tctcggcgga aatcttttct ttttccgcgt tatccgccga aataatgatg
     5761 taggcggcaa aatagatcac cttttccagc gccagcatcg gcaaattcag aacctggccg
     5821 atgcgcgagg gcactccccg caaaaaccag atgtgcgaca ccgggcaggc cagcttaatg
     5881 tggcccatcc gctcgcggcg cacgctggac ttggtcactt ccacgccgca gcgatcgcaa
     5941 acgatgccct tgtagcgcac ccgcttgtat ttgccgcaat aacattcata gtcccgctcc
     6001 ggcccgaaaa tgcgctcgca aaacaagccg tccttttccg gccgctgggt gcggtaattg
     6061 atggtttcgg gcttggtgat ctcgccgtgg gaccaggaaa cgacttcctc gggagacgcc
     6121 agcttgatcc gaattgattc taaatcttct acgcgcataa aatttaaaaa atccaaagct
     6181 caaatgtcaa atgccaaatc aaatccaaaa tttaaaatta aaattttaaa attgaattca
     6241 ttttgacatt aggattttag tcatttgttt gacatttgga ttttgacatt aggatttttt
     6301 gactttattg gttgtttctc tcttttactt ccacgctcaa tcccagcgat ttcaattcgc
     6361 tcaccaaaag gttgaacgac gccggaatgt tgggatggcg aatgtcttcg ccgcgcaaaa
     6421 tggtttcata ggttgccgcc ctgccggcga tatcatcgga tttgatggtg agcatttcct
     6481 gcaaagtgtg cgccgcgcca tatccttcca gcgcccagac ctccatttct ccgaaccgct
     6541 gtcccccgaa ctgcgccttg cctcccagcg gctgttgggt gatcagcgaa tacggcccga
     6601 tactgcgcgt gtggatcttg tcttccacca tatgcaccag cttcatcata tagatatagc
     6661 ccacggtgat cttgcccggg aacggcaatc ccgaccggcc gtcaaaaagc ttttctttgc
     6721 cgtcttcgga aaggccggcc tttttcaatt ccgctttgat gtcggcttcg gtcgcgcccg
     6781 aaagcgacgg cgtcaccgcg tgatagccga gtttgtgcgc cgcgtagccg aaatgagttt
     6841 ccaaaatctg ccccaggttc atgcgcgacg ccacgctcat gggattgagc acgatgtcca
     6901 cgggcgtgcc gtcttccaaa aacggcatct cttccaccgg caaaatgcgc gaaatcacgc
     6961 ccttgttgcc gtgccggccg gccagtttgt cgccggcgcg caccttgcgc aattccgcca
     7021 cctcaatctc aatgcgcttg atcacgcccg gatcaagctt gtgcccctgc tcgcgcgaga
     7081 aaatcttcac gccgacgacg cgtccccgct tgccgtgttc catcaccagc gaagtgtctt
     7141 tgacatcttt ggctttttcg ccgaaaattg agcgcaggag ccgctcttcg gcggtgagct
     7201 cctgttctcc tttgggggag atttttccca ccagaatgtc gttgggtttg atctccgccc
     7261 ccacgcgcac aacgccttct tcgtccaagt ctttcaattt ctcttcggaa acattgggga
     7321 tgtcataggt ggtggtttcc ggccccagtt tggtttcgcg cacgtcgcag gcgaaatttt
     7381 ccatatggat ggtggtgaat ttgtcatccg ccaccaaacg ctcggagagt aagatcgcgt
     7441 cttcaaaatt gccgccgcgc caggaagtga acgccaccaa aacgttgtgc cccagcgcca
     7501 gatggccgtc ggcgatgccg ccgccgtcgc aaagcgcgtc gcccttcttg actttttgtc
     7561 cttttttcac cgccgctttc tgatggaagc aggtatattg gttggtgcgc aaaaaggtgc
     7621 gcaaatagta agttttttcg cccagttcct tgtcgtcgta tttgacggcg atgtgccgcc
     7681 cgtccacttc ggtgatcacg cccgcgtctt cggccggcag aatatgcccc gaatcgcgcg
     7741 cgattctttc ttcactgccg gtcatcacca gcggcacttc gggcaacaac agcggcgttg
     7801 actggcgctg catattggaa cccatcagcg cgcggttggc gtcgtcgttc tgcaaaaacg
     7861 gaatcagcga cgtgcccacc gaaaggatct gctgggggga aacgtcaatg tagtcaattt
     7921 cttcgcgggc gcaaatgccc ggctccccct tgatgcgcgc ttccaatttt ttgggaatga
     7981 tgttgttgtt ctcgtccagc ggcgcgattg ccgtggcaat gttgtatttc tcttcctcgt
     8041 cggcggtcat atagaccagc ttgtcggtca ccttgttttt tttcacctca aaatagggag
     8101 tttccaaaaa gccgaaatcg ttgatcttgg tgtaggacgc caaataaccc accaggccga
     8161 cgttcgcgcc ttcgggagtt tggatcgggc agatgcggcc gtaatgggag ggttgcacgt
     8221 cgcgcacttc aaatcccgcg cgctcgcgcg tgagtccgcc ggggccggtg gccgccaggc
     8281 ggcgtttgtg ctccagctcg gaaagcggat tttcattgtc cataaattgc gacagctggg
     8341 aagatgagaa aaattccttg atcaccgcca tcaccggccg cggattgatt aactgcaggg
     8401 gcgacaattc caaaacatcc agagttgaca ttctgtcctt gatgatgcgc tccatgcgca
     8461 tgaagcccag ccgcaggcgg ttcaccagca gttcgttgag cgtgcgcacg cggcggttgc
     8521 ccaaatgatc aatttcgtcg ggtttggcgg cgggatcgtt gttgaggcga atgatttccc
     8581 tgattaccgc cacgatgtcg tccaccgtca tcacgcgatc cttcatttcc acgacgtcgt
     8641 ttttcttgtc tttgggtttc aattgcggca ggcgtttcca cattttccag cgcccgactt
     8701 tggaaagatc atatctttca aagttgaaaa acatattcca gatcagttcc ttggcggtgt
     8761 cggcggtggt caggtccccc gggcgcaggc gctgatagat ctccaccagc gcctcggcct
     8821 ggttgtgcgt gggatctttt ttgatggttt cttcaatgaa atcaacttcg ccgttgttga
     8881 cgtcggcaaa cagttccttg atcgcgttgt cgttgtcaat gccgaacgcc cgcagaagcg
     8941 tggtcgccgg cacgcgccgc ttgcggtcaa ttttcacgcc gatgaagccc gtgtgggcgg
     9001 tttcaaattc cagccacgaa ccgcggttgg gaatgatctt ggtgccgaaa aaattcttgc
     9061 cgccgatgtt ttgggcggag aaaaacacgc ccgccgagcg gattaattgc gagatgcaaa
     9121 cccgctccac gccgttgatc acaaacgtgc cccgctcggt catcaacgga aaatcgcaca
     9181 gaaatatctc ttgcgttttg gtttctttgg ttttcaggtt caccagccgc gttttgaccc
     9241 gcaccggcga ttcataggaa tcgttgttca ggcgcgcgtc cagatcgttc ttgtgtttgg
     9301 gcttgtccag cttgaaatcg tcaatccaaa gctccatttc cttgccggtg tagtcgcgga
     9361 tgggcgagat ctcggtgaac aattcccgca aatcgttgcc ccagaacata tcccagctct
     9421 ctttttgcaa caccagcaaa tacggcaggg gcaaagaaac tttcgccttg ccgaaatttt
     9481 ttgttttaag ggccatataa cttaaattaa aaattaaaaa atcaaagtta aaaattttgt
     9541 atttttgggg tctatccctc gtgtaatcgc gagggttaga cccctccaac agtattttta
     9601 actttgatct ttcaatttca aatttgaaac ggcaggcgcg gcaaaaaagc aaaaaaaccc
     9661 tctttggttt ttcagggcat attttgctta aaattatgaa tcaacggcaa tcaaggcaaa
     9721 aaaaatgagt tagctctccc acaagcattg atattagcaa acatttccca atctgtcaaa
     9781 tccacagggt cgttttgtga aatgataatc cccggggtca acggggtcgt tcccttttgt
     9841 cctcaaaagg gtacgacccc tttaaaaagt atcatttatc agttatcaat tatcagttat
     9901 catttatcat ttatcattta tcatttatca attatcaatt atcatttatc atttgaggcc
     9961 cgcaatcagt ccccggccgg ttttttggca atctcggaca ggacgaactc cttgattctt
    10021 tgcgccgccg cataagcgtc ttcggccttc ttcacccatt cctgccacaa ttgatccgcg
    10081 gggagtttat ctggcatata ataatttacc tttatcaata atatcaatta caaacgggtc
    10141 ctgcattttt ttcctcttgt ccacctgctc cggggtataa accaataaat ccatcgcaaa
    10201 ctcccgcggg aaaaaaagct tgtcaacatc ctccatccgc tttaacatcg gatcatcggt
    10261 ttttttgatg ataaaaagat caagatcgct gtcttcgtgc ggatgccccc acgcgtaaga
    10321 accgaaaaga atgatctttt cgggcttgta ttcgcgggcg atcttgtcgg caatcgcctg
    10381 tatttttcgc ttggtttctt ctttcaccat atcttcatcc taccaaaacc caaacccccg
    10441 tcaacctctc agaagatctg tgcaaaaatc agagggttag accctctgaa aaaaaagggt
    10501 ctatccctcg cgcttgtccc gaggtaatcc gagggataag cgcgagcggt tagaccctgg
    10561 caaaagaatt ttaaatccag tgagcggtta ccggccgccc gccgcgccca aagcgaagat
    10621 t
//
LOCUS       DAOQWE010000016        10518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_6779_length_10518_cov_6.858071, whole genome shotgun sequence.
ACCESSION   DAOQWE010000016 DAOQWE010000000
VERSION     DAOQWE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6779_length_10518_cov_6.858071"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1371
                     /gene="eno"
                     /locus_tag="PKK37_01190"
     CDS             115..1371
                     /gene="eno"
                     /locus_tag="PKK37_01190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNR81029.1"
                     /translation="MIVPSKYKIQKIRAREILDSRGNPALEVCVLTGAGEFCAGVPSG
                     ASTGAGEAVELRDGGEKFGGLGVAKAAANVNGVINPQVIGMDCRDQEAVDGKMREIDA
                     TPNKSRLGGNAMCGVSLACARAAAGAQKKPLYEHIAKLAKNKKIVLPKPCFNVINGGA
                     HAGSDLDFQEFMVCPQAQNFGDNLRLGAEIYRQLKKDLAAKYGKSSVNLGDEGGFAPP
                     IKIPKEALDCLCGAIAIVSPAVPVKFIVDVAATQFFDGVRYQTNFGGFAAGGLAQFYS
                     GLISQFPQIIGLEDPFAENDWEGWRGFSFSGMMIGDDLLVTNPQRMKEAKEKKACNAM
                     ILKINQIGTVSEALEAAKLAKEYGWKIIVSHRSGETNDDFIADFAAGISADYIKTGAP
                     ARGERVAKYNRLAKIEEELIANKAGL"
     gene            1368..4148
                     /locus_tag="PKK37_01195"
     CDS             1368..4148
                     /locus_tag="PKK37_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81030.1"
                     /translation="MSTIFNLIENKAAGWATGEKSKNRSVAGNVLNYIEKMDKLREPQ
                     KQAIAVYLWLKFIGNNQKLADIIRAGLLYDKDLAKNNDNFYTFGDDCAAQFLNQFFLE
                     NGVKNLRQKLINDPRGEKINWNNVLDDILHNYDYSNYLFSLPMGAGKTYLMASFIYLD
                     LYFAGMYKNDPRFAHNFIVLAPQASKTAILPSLQTIKNFNPEWILPKNVAVKLKQTIQ
                     IEILDALGSQRRDKLQGNNPNLEKVSRLAQTKDFGLVFITNAEKIVPERYDEKDKFYT
                     DPQSLLYDQKIAAQITKINELRERLSEIKNLGVILDEVHHSYGVNSQKEAKKLRRAIN
                     ILNQRKNVAGVLGFSGTPYVRHIVKIADEEIKLSQIQDIVYNYYLNDGIGKFLKVPEI
                     KKADVKESLFIGRALDDFFAGYDIKYKNGARSKIVFYCPLIKTLNEKILPEIRDWYAR
                     NRPGKENEIFKFYANAGKENKQYEISKDSLAIFNNLDKPYSDKRVILLVGIGGEGWDC
                     KSLSGVVLPRQKTAKNFVLQTTCRCLREMDDAQNEKALIYLEKENYKTLDNELRENYR
                     LKISDLKFAGQNRIPVAVRKPKLGKLNYKQVKARYEIVAKKTFAGSLAEFDFEKIREK
                     YSYVADITTGRIGRAGLIGEVVKKNRQADSADYSLTDFIYDLARATYGKYGEADLLTG
                     HKKDIQAIFDVIKDNFDWINANPRLEMDDIIKYAASLLMEEIEYKKEIIAENAQIELL
                     EWLGANPAIAYGAESDFRRFLPEVTPRLAEIYKKHRTVLDEALAEIDPQDAGFNYAPY
                     KFDSQFEINALREMLKLSDLKDKGLEIYYNGYNENRDLQSFFIKTADGVYTPDFLVVR
                     RAGKKIAKVLIVETKGKIYDDEDFRKKERFVKNEFRTHNPDFSYVKFVDAKGDNDFSR
                     FMGELRDIIKTF"
     gene            4153..>5628
                     /locus_tag="PKK37_01200"
     CDS             4153..>5628
                     /locus_tag="PKK37_01200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009218179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81031.1"
                     /translation="MAKPKSYNISPARGQALLNYQGRLMPNANLEMRLFEAETIEQVA
                     AAENGKPARQEKELNADFGNLLLQGDCLSACAYLKSKNIKPDLVYIDPPFASGANYAK
                     KIYLRNGQKTSFENDSASIGEEIMYGDIWQKEDYLNWLYERLLAIREVMAETASIYVH
                     LDWHIGHYAKILMDEVFGEENFRNEIIWRYGKMASSNKKFISNHDIIFLYSKTDSNIF
                     NVIKTKLDIPIKRLARTVMDGKLVNAKDKNGKTFHVEYNDKIIDDVWDDISIAMPASS
                     QFVDYATQKPEALLERIIKASSDEGMIVADFFSGSGTTAKIAHDLGRKFVACDIGVNA
                     IQTARDRLAKAGAGFEILKIKDGVRLFRNPAQTAQKIFSLVEGFSPRTDLDLGEFWDG
                     GIAAGKSAYAPVKFIGIDKKLTKPLVDAILEEVYVLEDAPGATAQVKIIYAHKDFEVD
                     QSYVDKEIAKAGKTTIAVELIGLDDLLAEKRDLLFAPDSAQI"
     assembly_gap    5629..5728
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5857..6600
                     /gene="gpmA"
                     /locus_tag="PKK37_01205"
     CDS             5857..6600
                     /gene="gpmA"
                     /locus_tag="PKK37_01205"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNR81032.1"
                     /translation="MYKIVLLRHGESVWNKENRFTGWTDVGLSELGIREAKDAGQVLK
                     KEGYVFDVVFTSVLKRAIDTTNIVLNEMGLSVIDTRCAWQLNERHYGALQGLNKGEMA
                     KKYGEEQVRIWRRSYGVRPPEMEEEDYRRQNELEVFKSVPVYEMPRTESLSDTYGRTI
                     DYWNSDIAPAVRDGKKILISAHGNSLRALAKYLDNIGDKEISELNIPTGVPLVYELDD
                     DLKPIRHYYLGDQDAIAKKIDAVKNQYKI"
     assembly_gap    6965..7064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7065..8225
                     /locus_tag="PKK37_01210"
     CDS             <7065..8225
                     /locus_tag="PKK37_01210"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HNR81033.1"
                     /translation="VPVRGGRSQKEIKNGFDDLVFVPAQLAKCPVDYFKEKISSKTIL
                     GKNSLRPVEIETPILIAAMSFGALSKNAKIALAKGSARAGTVANTGEGGMLPEERQFA
                     DRLTIQYSTGRFGIDEEILKKADIVEIKIGQGAKGGQGGLLQKEKLTAEVAAVRGVKM
                     GLDVHSPAYHCDIKNADDLKAKVDWLRGLSGGVPIAVKLAAGDIKKDVAVAVKANPDI
                     IAIDGGWGGTGAAPEVMLDETGIPALPALIAAREILDDMNMKVKAKQELWIAGGFYTG
                     GDVAKALALGADAVFMGSAMLAALGCVGCNLCYTGQCPKGVATQDPELAKNIDVEAAA
                     NNVANFIKHCTEEVKMIAGATGHNDIHNLNKDDLRSLTAEVSQITGVKPAGE"
     gene            8412..8789
                     /locus_tag="PKK37_01215"
     CDS             8412..8789
                     /locus_tag="PKK37_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81034.1"
                     /translation="MIGLFFEWYFNEIPVKIKKIWGNYLWFFSRYFAIPDLAREFFAP
                     WKGIMFQREKLGLDIGDILSAWFGNVISRIIGAIIRTFFLFVGIAVEIFAFLAGIAVY
                     VFWGAAIVLVPASLVFGFYLLFY"
     gene            8806..>10518
                     /locus_tag="PKK37_01220"
     CDS             8806..>10518
                     /locus_tag="PKK37_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81035.1"
                     /translation="MANNLKYPSTFRAIDNVAAVPANVFAWTRRISWFFLLWSLMIAG
                     ALGLISLIPEEFSGLILGKIPFSLPEIKVMPFLGVALIALFFWIPSNLVLGFVRQKLM
                     RAGRDLDPDLPRTEGDLLENLDFEAAGIVKSAISLSNLPAAKVLLYKILRRRRLDFAL
                     KRLMIDDQTFAGDLLAEMKANSVPLSKQNDSALRAANQAALFGILEKAARLSLENEEK
                     RISIFTIFLTIVNEDEKFQRLMDSMQMLKEDLWSVVLWQMRMEGYRAFRAKFWERDNL
                     RRAFMTSPVDMVVGGYTVLLDQYSHDIALTNPLRQGGVVLHMHEIEQLEEALIKQRGN
                     GILLVGESGGGRKSVVYNFANRVAAEQGPAALKMLRILEVNMVALIGNNPDKATLAAV
                     LDGIFAEAIRAQNVVLVIPQIHNYIGRGHGGDAVATIDISGVIGTYLKRPGFRLIGIA
                     DYEGLHRSIEQAGEIAAQFTKIEVERVSIADTMRVLKEEGLRREKDSNIFIPYASLKE
                     VAKLCDYFQGDMAFPLKAVNLLDDLIANKVNYGGKEAKTILPADVDEYFSRKYQVPAG
                     AAGQR"
BASE COUNT         2884 a         2078 c         2782 g         2574 t
ORIGIN      
        1 taaataaaca gcgtcatgaa attatcaaat gccttaatca aaatcctagt aatttttggg
       61 taaataccgc tggatttgag gcgttaatca gcgattttaa aaaataaatc catgatgata
      121 gttccaagca aatataaaat acaaaaaatc agggcgagag agattttgga ttcaagggga
      181 aatccggctt tggaagtctg cgttttgacc ggcgcgggcg agttttgcgc cggcgtaccg
      241 tcgggcgcgt ccacgggtgc gggcgaggcg gttgaattgc gggacggcgg cgaaaaattc
      301 ggcggattgg gagtcgccaa agccgcggcc aatgtcaatg gcgtgattaa tccccaagtt
      361 atcggaatgg actgccgcga tcaggaggcg gtggacggaa aaatgcgcga gattgatgcc
      421 acaccgaata aaagccgcct gggagggaat gcaatgtgcg gggtgtcgct ggcatgcgcg
      481 cgcgccgcgg cgggcgcgca aaaaaaaccg ctttacgaac acatcgccaa gttggcaaaa
      541 aacaagaaaa ttgttttgcc caagccgtgt tttaacgtta ttaacggcgg cgcgcacgcg
      601 ggcagcgatt tggattttca ggaatttatg gtttgcccgc aagcccaaaa tttcggcgat
      661 aatctgcgcc ttggcgccga aatttaccgg caattgaaaa aagatttggc ggccaaatac
      721 ggcaagtcaa gcgtgaattt gggtgatgaa gggggattcg cgccgccgat taaaattccc
      781 aaagaggcgc tggattgttt gtgcggcgcg attgcgatcg tatcgcccgc ggtgccggtg
      841 aaattcattg ttgacgttgc cgccacccaa ttttttgacg gcgtccggta tcagaccaat
      901 ttcggcggtt ttgccgccgg tggattggcg caattctatt cggggttgat ttcgcaattt
      961 ccgcagatca tcggcttgga agatccgttc gcggaaaatg attgggaggg ctggcgcgga
     1021 ttttctttca gcgggatgat gatcggcgat gatttgctgg tgaccaatcc gcaaagaatg
     1081 aaagaagcca aagaaaagaa ggcgtgcaac gcgatgattt tgaaaatcaa tcaaatcggc
     1141 acggtgagcg aggcgctgga agcggccaaa ttggcaaaag aatatggctg gaaaattatt
     1201 gtgtcgcatc gttccggcga aaccaacgat gatttcatcg ccgattttgc cgcgggcatt
     1261 agcgccgact acattaaaac cggcgcgccc gcccgcggtg aacgggttgc caaatacaac
     1321 cgattggcaa aaattgaaga agaattgatt gctaataagg cggggttatg agcacgattt
     1381 ttaatctaat tgaaaacaaa gcggccgggt gggcaactgg tgaaaaatca aaaaaccggt
     1441 cggtggcggg taatgttttg aactacattg aaaagatgga caaattgcgc gaaccgcaaa
     1501 aacaggcgat tgcggtttat ctttggttga aattcatcgg caataaccaa aaattggctg
     1561 atattatccg cgccggttta ctgtatgaca aggatttggc aaaaaacaat gataattttt
     1621 acacattcgg cgatgattgc gccgcgcaat ttttgaatca attttttttg gaaaacggcg
     1681 ttaaaaattt gcggcaaaaa ttgattaacg atcccagggg cgaaaagata aattggaata
     1741 atgttttgga cgatattttg cacaattatg attattccaa ttatttgttt tcattgccga
     1801 tgggcgcggg taagacatat ttgatggcga gttttattta tttggatttg tattttgccg
     1861 ggatgtacaa aaatgatccg cgatttgccc acaatttcat tgtattggcg ccgcaggcat
     1921 caaaaaccgc gatcttgccg tctttgcaaa ctatcaaaaa ttttaatccc gagtggatat
     1981 tgccaaaaaa tgttgccgta aaattaaagc aaacaatcca aatagagatt cttgacgcgc
     2041 ttggctccca gcgcagggat aagttgcaag gcaataatcc caatttggaa aaggtaagcc
     2101 gtttggcgca aaccaaggat tttggcttgg tttttatcac caatgccgaa aaaatcgtgc
     2161 cggagaggta tgatgagaaa gataaatttt ataccgaccc ccaaagcttg ctttacgacc
     2221 aaaaaatcgc cgcgcagata actaaaatca atgaattgcg cgagcggctt tcggaaataa
     2281 agaatttagg cgtgattttg gacgaagtgc accatagtta tggcgtaaat tcgcaaaaag
     2341 aagcgaaaaa attgcggaga gcgataaata ttttaaacca gcgcaagaat gttgcgggtg
     2401 ttttggggtt ttccggcacg ccctatgttc gccatattgt gaaaatcgcc gatgaagaaa
     2461 taaaattaag ccagatacaa gatattgttt ataattacta tttaaatgac ggcatcggga
     2521 aatttttgaa agtgcccgaa atcaagaaag ccgatgtcaa agagagtttg ttcatcggcc
     2581 gggcattgga tgattttttc gccggttacg acattaaata caagaatggc gccagaagca
     2641 aaatcgtttt ttattgcccg ttgataaaga ccttaaacga aaagatactg cccgaaatcc
     2701 gggactggta tgcgcgcaac cggccgggca aggaaaatga aatttttaaa ttttatgcca
     2761 atgcgggcaa agaaaacaag caatacgaaa tatccaaaga cagtctggcg atattcaata
     2821 atcttgacaa gccttattcg gataaaagag tgattctgct ggttgggatt ggcggcgaag
     2881 gctgggactg caagagtctg tcgggcgtgg ttttgccgcg ccaaaagaca gccaaaaatt
     2941 ttgttttgca aacgacctgc cgctgtttgc gcgaaatgga tgacgcgcaa aacgaaaaag
     3001 cgttgattta cttggaaaaa gaaaattaca agactttgga taacgaattg agagaaaatt
     3061 accggctgaa aatatccgat ttaaaatttg ccggacaaaa tagaattccg gtggcggtgc
     3121 gcaagccaaa actgggcaaa ctgaattaca agcaggtaaa ggcaagatat gagattgtcg
     3181 ccaaaaaaac ttttgccggc agtttggctg aatttgactt tgaaaaaatc agggaaaaat
     3241 acagttatgt tgctgatatt accacaggca gaattggaag agccggtttg attggcgaag
     3301 ttgttaaaaa gaatcgacaa gccgatagcg ccgactattc actgactgat ttcatctatg
     3361 atttggcgcg ggcgacttat ggcaagtatg gcgaagcgga tctgctgacg gggcataaga
     3421 aagatataca ggctattttt gatgtcataa aagataactt tgactggata aatgccaacc
     3481 cgcggctgga aatggacgat ataataaaat acgccgcttc gttgctaatg gaagagattg
     3541 aatataaaaa agagatcatc gccgaaaatg cccaaattga attgctggaa tggcttggcg
     3601 caaatccggc gattgcctat ggcgcggaaa gcgatttccg ccggtttttg cctgaagtta
     3661 caccgcggct ggccgaaatc tataaaaaac accgcacggt attggacgag gcgctggccg
     3721 agattgatcc gcaagacgcc ggctttaatt acgcgcccta taagtttgac agccagtttg
     3781 agatcaacgc tttgcgcgaa atgctgaaat taagcgattt aaaagacaag gggctggaga
     3841 tttattacaa cgggtacaac gaaaaccgcg atttgcagag ttttttcatt aaaacggctg
     3901 atggcgtcta tactcccgat tttttggttg tccggcgcgc cggcaaaaaa atcgccaagg
     3961 ttttgattgt ggaaaccaaa ggaaagatat atgatgatga agacttccgc aaaaaagagc
     4021 ggtttgtgaa aaacgaattc cgaacgcaca acccggattt ttcctatgtg aaattcgttg
     4081 acgccaaagg cgacaatgat tttagccggt ttatgggcga attgcgggac atcatcaaaa
     4141 ctttttaaaa ttatggcaaa accaaaatct tacaatattt cgccggcgcg cggccaggcc
     4201 ctgcttaact accaagggcg gctgatgcca aacgcaaatc tggaaatgcg gctgtttgaa
     4261 gccgagacca ttgagcaagt tgcggccgcg gaaaatggaa aaccggcgcg gcaagaaaaa
     4321 gaattgaacg ccgattttgg gaatttgctg ttgcagggcg attgcttgtc ggcgtgcgct
     4381 tatttgaaat ccaagaatat caagccggat ttggtttata ttgatccgcc gtttgccagc
     4441 ggcgccaatt acgccaaaaa aatctatttg cgcaacggcc aaaaaacaag ttttgaaaac
     4501 gacagtgcca gcatcggcga agaaataatg tatggcgata tctggcaaaa ggaggattat
     4561 ttaaactggc tctatgagcg gcttttggcg atccgcgagg tgatggccga aaccgccagc
     4621 atctacgtgc atcttgactg gcacatcggc cattatgcca aaattttaat ggacgaagtt
     4681 ttcggcgaag agaattttag gaatgaaata atttggagat atgggaaaat ggcatcgtcc
     4741 aataaaaaat ttattagtaa tcatgacatc atttttctat attcaaaaac agatagtaac
     4801 atttttaatg taataaaaac aaaattagac atacctataa aacgacttgc cagaacagtt
     4861 atggatggta aattagtgaa tgcaaaagat aaaaatggaa aaacttttca cgttgaatat
     4921 aatgataaaa taattgatga tgtttgggat gatatatcta ttgctatgcc tgctagtagt
     4981 cagtttgttg attatgcgac acaaaagccc gaggcgttgc tggagcggat tattaaggcg
     5041 agttccgatg aggggatgat cgtggcggat tttttcagcg ggagcgggac cacggccaaa
     5101 attgcccatg atttggggcg caagtttgtg gcctgcgaca tcggcgttaa tgccattcaa
     5161 actgcgcggg atcgtctggc caaggcgggc gcaggctttg agattttgaa aatcaaagac
     5221 ggcgtgcggc tgttccgcaa tccggcgcag accgcgcaaa agattttttc gctggttgaa
     5281 ggtttttcgc cgcgaacaga ccttgatttg ggcgaatttt gggacggcgg cattgccgcc
     5341 ggcaaatccg cctacgcgcc ggtgaaattc atcggcattg acaaaaagct taccaagccg
     5401 ctggtggacg cgattttgga agaagtatat gttttggagg acgcacccgg ggcgaccgcg
     5461 caagtgaaaa ttatttacgc ccataaagat tttgaggttg atcaaagcta tgtggataaa
     5521 gaaatcgcca aagccggcaa aaccaccatt gccgtggaac tgatcggctt ggatgatttg
     5581 ctggccgaaa agcgtgattt gcttttcgcg cctgattcgg cgcaaatcnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga attggaagac aaagccggcc ccaaagataa
     5761 aatcaaggcc atatatgaat tgccgggcga taagtttaag atcaagatcc gcaatattgc
     5821 cggcgatgaa accatttttg attattccaa ataattatgt acaaaattgt gttgctaagg
     5881 catggggagt cggtatggaa caaggagaat cggtttaccg gctggactga tgttggttta
     5941 tctgaattgg ggatccgaga agctaaagac gccggccagg tcctgaaaaa agagggctat
     6001 gtttttgatg tggtttttac ctcggttttg aagcgcgcca tcgatacgac aaatatcgtc
     6061 ttaaacgaga tgggtttatc cgttattgat accagatgcg cttggcagct gaatgagcgg
     6121 cattatgggg ccttgcaagg tttgaataag ggcgagatgg cgaaaaaata tggcgaagaa
     6181 caggtgcgga tatggcggcg cagttatggc gtccggccgc cggagatgga agaagaagat
     6241 taccgccggc agaacgaact ggaagttttt aaatccgtgc cggtttatga aatgccgcgc
     6301 accgaatcat tgagcgatac ttatggccgc acaatagatt attggaattc agatatcgcg
     6361 cccgcggtca gggacggcaa aaagatactg atttcggcgc atggcaacag tttgcgcgcg
     6421 ctggccaaat atctggacaa catcggcgac aaggaaatct cggagctaaa cattcccacc
     6481 ggcgtgccgc tggtctatga actggatgac gatctaaagc ccatcaggca ctattatctg
     6541 ggcgaccaag acgcaattgc caaaaagatt gacgcggtca aaaaccaata caaaatttga
     6601 aatttggcgg acattccaac attctcaaga atgttggaat gtctgtgatg gttttattgg
     6661 cgatattccg atattctgca gaataccaga atataataat ggtggaattt gggcgggcga
     6721 gcgaataagt cttgttttaa tttttaaaat tggtagaatg gcggtgtagc ttaagttgta
     6781 aaataataaa aatatggcaa aataccaggt gaacaaagac aaatgtttgg gatgctttat
     6841 ttgcaccgtg aactgtccgg cgggaatggc gcaggatgac gatgccaagg ccaaggtggt
     6901 ggattccgac gcggtggaaa attgcggcgg cgaaagcgtc tgcccccacg gcgtgattga
     6961 aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgccg gtacgcggcg
     7081 ggcggtcgca aaaagaaatc aaaaacgggt ttgacgattt ggtgtttgtt ccggcgcagt
     7141 tggcgaaatg ccccgtggat tattttaaag aaaaaatttc ttccaaaacg attttgggaa
     7201 aaaacagttt gcggccggta gaaattgaaa cgccgatatt gatcgcggcg atgtcttttg
     7261 gcgcgctgtc aaaaaatgcc aaaatcgcgc tggcgaaagg gtcggcgcgg gcgggtaccg
     7321 ttgccaacac cggcgagggc ggaatgttgc ccgaagaacg gcaatttgcc gatagattga
     7381 caattcaata cagcacgggc cggtttggca ttgacgagga aattttaaag aaggccgata
     7441 ttgttgaaat taaaatcgga caaggggcca aaggcggcca gggcgggctt ttgcaaaaag
     7501 aaaaactgac cgccgaggtg gcggcagtca ggggcgtgaa aatggggctg gatgtccatt
     7561 cgccggccta tcactgcgat attaaaaatg ccgatgatct gaaagccaag gttgattggt
     7621 tgcgcggact gtcgggcggc gtgccgattg cggtcaaatt ggcggcgggc gatatcaaaa
     7681 aagatgttgc cgtcgccgtt aaagcaaatc ccgatatcat tgccattgac ggcgggtggg
     7741 gcggcacggg cgcggcgccg gaagtgatgt tggacgaaac gggcattccc gctttgccgg
     7801 cactaattgc cgcgcgcgaa attttggatg atatgaatat gaaggtgaag gcgaaacaag
     7861 aattgtggat tgccggcggg ttttacaccg gcggggatgt tgccaaagcg ctggcgctgg
     7921 gcgcggatgc cgtgtttatg gggtcggcga tgttggccgc gctggggtgc gtggggtgca
     7981 atttatgtta taccggccaa tgcccaaagg gggtggccac gcaggatccc gaattggcga
     8041 aaaatattga tgttgaagcg gcggcaaaca atgttgccaa ctttatcaaa cattgcaccg
     8101 aagaagtgaa aatgatcgct ggcgccaccg gccataacga tatccacaat ctcaacaaag
     8161 acgatctgcg ctccctgacc gccgaagttt ctcaaatcac cggcgtcaaa cccgccggcg
     8221 aataataaac taatcgcaag aaataagtgg aagtcaaacc tccactacct ccgctcattt
     8281 catttttgtt aaataattta gcggccgcta aaaaaatcac acttttgcat caaacgcgga
     8341 ttttttgttt tttcggggga tatttggtat aatgccgtaa tatctgaatt tattcccata
     8401 tttttatatt tatgatcgga ttgttttttg agtggtattt taacgaaatt cccgttaaga
     8461 ttaaaaaaat ctgggggaat tatttgtggt tcttttcccg ttattttgcg attcccgatt
     8521 tagcgcggga atttttcgct ccctggaagg gaatcatgtt ccagcgcgaa aaactgggtt
     8581 tggatatcgg cgacattctt tcggcgtggt tcggcaatgt gatttcacgg attatcggcg
     8641 cgatcatccg gacgtttttc ctgttcgtcg gcattgccgt tgaaattttt gcttttcttg
     8701 ccggcatcgc ggtttatgtt ttttgggggg cggcgattgt gctggtgccg gcatcgcttg
     8761 ttttcggatt ttatctgctt ttttattgat ctttgaagta atcttatggc taacaatttg
     8821 aagtatcctt ccacttttcg cgcgattgac aatgttgccg cggtgccggc aaacgttttt
     8881 gcatggacca gaagaatcag ctggtttttt ctgctttggt cgttgatgat cgcgggcgcg
     8941 ctgggattga tttccctgat tcccgaagag ttttccgggc ttattttggg aaaaatcccg
     9001 ttttctctgc ccgaaatcaa agtgatgccg tttttgggag tcgcccttat cgcgcttttt
     9061 ttctggattc cgagcaattt ggtgctgggg ttcgttcgtc agaaattgat gcgggcgggc
     9121 cgcgatttgg atccggattt gcctcggacg gaaggcgatt tgctggaaaa tcttgatttt
     9181 gaggcggccg gaatcgtcaa atccgcgata agtctctcaa acttgcctgc ggcgaaagtc
     9241 ttgctctata aaatactccg ccgccggcgc cttgattttg ccctcaaacg cctgatgatt
     9301 gacgatcaaa cttttgccgg ggatcttttg gcggaaatga aggctaattc cgtcccgctt
     9361 tcaaagcaaa acgattcggc cctgcgggcg gcgaatcaag ccgcgctttt cggaattttg
     9421 gaaaaagcgg cccggctgtc cctggaaaac gaagaaaaaa gaatctcaat cttcacgatc
     9481 tttttgacga ttgtcaacga ggatgaaaaa ttccagcgct tgatggattc aatgcaaatg
     9541 ctgaaagaag atctctggtc ggtggtcttg tggcaaatga gaatggaagg atatcgtgcc
     9601 ttccgggcca agttttggga gcgggacaat ttgagaaggg cgtttatgac cagtcccgtg
     9661 gatatggtgg tgggcggata caccgtgctt ctggatcaat attcccatga cattgcgctt
     9721 accaatccgt tgcgtcaggg aggcgtggtt ttgcatatgc acgagattga acaattggaa
     9781 gaggcgttaa tcaagcaaag gggcaacggc attttgctgg tgggggaaag cggcggcggc
     9841 cgaaagagcg ttgtctataa ttttgccaat cgcgtggcgg ccgaacaagg ccccgccgct
     9901 ttaaaaatgt tgaggattct ggaggttaat atggtggcgc taatcggcaa caatcccgac
     9961 aaggcgacgc tggccgcggt gctggacggg attttcgcgg aagcgatccg ggcgcaaaat
    10021 gtggtgttgg tcattcctca aatccacaat tatatcggcc gtggccacgg cggcgatgcc
    10081 gtggccacaa ttgatatcag cggcgttatc ggcacatatt taaaacggcc gggattccgc
    10141 ctgatcggca ttgccgatta cgaaggatta caccggtcga ttgagcaagc cggcgaaatt
    10201 gcggcacaat tcaccaaaat tgaagtggag cgcgtttcca ttgccgatac gatgagagtc
    10261 ctcaaagaag aagggttgcg ccgggaaaaa gattccaata tcttcattcc ttatgcgagc
    10321 ttgaaggaag tggccaagct ttgcgattat tttcaagggg atatggcatt tcccctgaaa
    10381 gccgtcaatc ttttggatga tttgatcgcc aacaaggtga actacggcgg caaagaagca
    10441 aaaaccattt taccggccga tgtggacgaa tatttctccc gcaaatatca ggttcccgcg
    10501 ggcgcggccg gacagcgg
//
LOCUS       DAOQWE010000017        10064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_7166_length_10064_cov_8.143371, whole genome shotgun sequence.
ACCESSION   DAOQWE010000017 DAOQWE010000000
VERSION     DAOQWE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7166_length_10064_cov_8.143371"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..545)
                     /locus_tag="PKK37_01225"
                     /pseudo
     CDS             complement(187..545)
                     /locus_tag="PKK37_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017087132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
     gene            complement(788..1168)
                     /locus_tag="PKK37_01230"
     CDS             complement(788..1168)
                     /locus_tag="PKK37_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81036.1"
                     /translation="MAKIRYSLTGPFEADLKRLLKRYGSLLADIEKAKVYAMEAYHLN
                     LIDRRAIFPIRGFCFDNVIVCKLKKFACKSLKNRGNRSGIRIIYAFYPAEPKIEFIEI
                     YHKSDQANEDKSRIKEYLKQKQQK"
     gene            complement(1149..1928)
                     /locus_tag="PKK37_01235"
     CDS             complement(1149..1928)
                     /locus_tag="PKK37_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HNR81037.1"
                     /translation="MNPNYIKKLREKNQLTQEYLAAQIGVSRPTYAQIEQGQRGLTVT
                     EAKKMADIFSLALDDFLAEKNLTVNIKPDKKSKNKSNDIRIDIPQEYADKFREVLLYI
                     LKKIGGKPNVGMTVLYKILYFIDFDYYEKYEEQLMGALYIKNNFGPTPVMFEGIMRDL
                     VKENKVEPIKSKFYQYPQTKYIINPKVEPDLSVIDGREKEHIDWELERLSDMTANEIS
                     NLSHKDVPWMMGELGKPMHYEAVFYRTDDTSVRKYDGEDSL"
     gene            complement(2089..3183)
                     /locus_tag="PKK37_01240"
     CDS             complement(2089..3183)
                     /locus_tag="PKK37_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81038.1"
                     /translation="MKPKILILLAVILLVIAAAAGFLWMSARKAAPLSENMPLSVSND
                     GDETPPAAPEPQTIAPLRLDYELKNFGPGQNITLSYFFEKKQTCARGAAYLGVAKLVS
                     SDGRFNNQYAKLAAYEDNGELGLSTWTNEGSLAFDDAVPYFNEMNVPLFVSELFIAAG
                     KNFNDEEYWRSGAPVILKDVIFGRSKGNYSIIYQGEDAAAAIPCTKFKIVGKTTNMDG
                     YFNVCVAKKINEVNLPFMVYMEFENEQGPQWQLKNAAKEESGVAWTPQCLEAVKCPYV
                     AEPASAERSRCAGQIEPKNDSNGCVEEYRCVSEIDRAKEAIARAQNPDCAIDQKLLEK
                     YLACRKNNQPNYDPVKYGEDGCLRDINCRP"
     gene            complement(3228..3890)
                     /locus_tag="PKK37_01245"
     CDS             complement(3228..3890)
                     /locus_tag="PKK37_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR81039.1"
                     /translation="MALQPFTDNFADNSTEAGFQFTFFCAICNEGYKTKFIESKTYNK
                     GKLLKGIGGLIGAAAQIAGKYNVGYGVDRGMDAIGERFNGMSPEWQKEHDKAFEQAQS
                     EAKAHFHRCPQCTQWVCENDWNEQENLCTQCAPRENVQIASARAKKKAADIDAKAAAA
                     KVFTGEIEAKQTLCPKCGKPAGTGKFCNNCGQSLAMAVCEKCGAKNPAGTAFCGECGK
                     KL"
     gene            complement(3937..5757)
                     /locus_tag="PKK37_01250"
     CDS             complement(3937..5757)
                     /locus_tag="PKK37_01250"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNR81040.1"
                     /translation="MKNKKTVCGAILAGCLSLAGMSVFAADAITGGGDFDSAVLVAPG
                     VYEVAALPEDHRDFFYFNLDSGQEAQVSVKFNQAAGSNYGTNVGFNLYDSERRQLRND
                     FGGTGDTFEFSYLSGSGAPGSKYYLETYNDGIWDGGSYVVTFKTADRYDAESKTDAGG
                     DIAGAMAISPGNHKGYLAGGDSFESGGDDLADFYKFNVSSGQSLNINAVPSLEASILL
                     AIYDTQRKLIDEVSVDNPGQIINLPFTAQKGGEVYLAVACYNEYCGERLASYDLGIGA
                     AVGGGAEIAVPDDGSDVVPPGGDTGTGSGGIGAGGMVGQNGDGEIFYDDDGNAMLSHE
                     QAGGVDMLFGGPGISTAFIIGNLIVLGVILFFSLIFYIYSAVCLQKIAKRTNTAPAWL
                     AWVPIANAFLMLKIAQKPWWWFFLLFVPVINIVAAVIVWMKIAEFTGKESWVGALTLV
                     PVIGIAIPAYLAFSGDSQKKTDGGEPEEKKGPEFIGGSKEADKPAVGYKHPCKYCEQM
                     VSPDAAVCPFCGKEGPLGPDRCPKCHDPIDKKWKACAHCGLNLRIVCPFCGKVTFFGK
                     YCEDCGKKLVVACPVCQFEQPPLGDKCIKCGQPLKGSAQK"
     gene            complement(5846..7399)
                     /locus_tag="PKK37_01255"
     CDS             complement(5846..7399)
                     /locus_tag="PKK37_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81041.1"
                     /translation="MRHKAKIFAVLAIGLFAVLPLVLAQPTDNSAGNGAAAESGENLY
                     GQGDEILDMTGYGQAIEALMKAPTGKMTDEIYVEITARLAVCHKGTMVTDEVFNHLKD
                     PYDVTAPEYAAFTYQNMERYMEEIDAAESDPAGGWIKKYEARKAELEANGCVFNTTPD
                     GEIKSPCMDADGDDPDREGEVYYYGETARAGNEHYVCEPCRYADACRGDFVVEQICRD
                     GKPADVEHRCEYGCFAGRCLAREESQKQLCEGQCLENCPAGTMDFGAGGCPKKDGQKN
                     VCCQKVFGKPEASGTCAGSCMKSSVCPEGYENKGTDSCVQEENCYKCGLFNLFTCCDY
                     TKNVCCVPKTDAPLPAPAKCNGYCAAGACAAGSFAKGTADCAPARTCLPCGFFGLKKC
                     CEYQSTICCEYPEPVTDCSGGICTYDECPSGTVDVGIANCGKEKKCKTACWGFCKKCD
                     KKQTRCCMAVGGEKCDRGTCQESGTCPVGYKSIGAADCGSYEKEYACGFLGMSKCKDP
                     AAKTCCVPE"
     gene            complement(7389..8777)
                     /locus_tag="PKK37_01260"
     CDS             complement(7389..8777)
                     /locus_tag="PKK37_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81042.1"
                     /translation="MDHSNPEDANREVPAADAEVKNKVLFKTAGDYAILTAAGAEQER
                     GAAKIILKEEEISFLPDNGKALNIDIREIDQAAADDYRVLMNLKSGAAVEISRLGYQF
                     EDFWRHFSAAWNAVLIDIFLMKEAADKGSARARVLRSGKDLGECQIQFYETSFLVLPR
                     ESGLFKIFYGDVDEMKAQDFKIALSAEEENIELSQMGQDFDFCAKAVNAGMTAILLNA
                     QTQIKEMIPGLDSLGVRQLAEVMRDGRCAAKSRVDSLAPGTWEKIEDFLQAAGMKEEY
                     DYLKSLAGGGEIYAGVKRGAMGSLSGDYAWFLVPLKNNNAIAMEASSEGGSGKATYFF
                     RLVAPLEFEAKKNDLDREVQKTAAAVQKCVRRINFRREPIYLPEEKLQDPKYWKYKFA
                     LAQIPELRFLREIFIGRALHRSPEIWRKDVQKLLEFNAAAKDGAKWRADGDDLEPPAV
                     PEQNNAQNQNET"
     gene            complement(8819..9922)
                     /locus_tag="PKK37_01265"
     CDS             complement(8819..9922)
                     /locus_tag="PKK37_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81043.1"
                     /translation="MEQKKTKTSFLKKIGEFFKNNKPAKVAGLAVLGLAGIYLVINLP
                     RTALPAVLPAMPGYYPSSEYKSSGYGDDYAFSEKYNSVPLASSMELPMESGGGISGLF
                     SARNAASLADDGGGTAGDAAENYETAEYRVNYESRDLEKTCAAVGGLKPRPEVIFENA
                     VESDEYCRYTFKVQRAKAEEILGFLKGLGPKDVTENIYTIQERLEDYTGQIDILEKKL
                     AVIDDTLNRANAAYDEVSGLATEQNDAETLSKVINNKINTIKQLTQERLNTAAQLEQL
                     RRSKSLQADKLDYTYFNVYARDNSYFDWRDVKDSWQTAVKNAVGGLNRIVQSISVNLA
                     IFLLEILQYAIYGAVLLIAAKYFWKAAKAIWKK"
BASE COUNT         2348 a         2820 c         2187 g         2709 t
ORIGIN      
        1 cgccaagccc catcttctgc cgcccagcct caaaaaactc atcatacccc aaatcctcga
       61 tctttatccg gccattctca ttatccatac aaattacttt aacataaact acatcactta
      121 tcatcggttt gttcaattac cggaataccc cagggcttta ccccgagata gtttatttta
      181 aaatcgttat cgaaataacg acaattttcc ggtaatctct tttaattcca tagtcttgcc
      241 aaacagacaa ataccataat attccaaatt tgtttccaaa gtttccccag tagcaaacat
      301 ttgctgttcg gaagtgccga tagccatcgt ggaagtaaaa tcaacaaacg gaatatttct
      361 tttgagcgcc tcattgcgca ccgcgcggat cttgttggaa ttatcagctt gcaaaacaat
      421 aaatgggaaa tgagaaatat tgggatgctt gccgccgttt ttatcttcat actgcaaaaa
      481 acacagctca tcccgcaacc caacccccct gctaaacctg cggtcatatg tcccagtgcg
      541 ttcattaatc tccctatttc aatcttttta tttatctgcc agaatacccc gtcggctttg
      601 ccgcggggat gaatggctgc ccgtagcgta gcgaagcgga gcggaggcta taaacaaact
      661 tttcctaccg cgtagcgtaa gcggagcgga aggaatacct cggggctctg ccccggggta
      721 gtttattcag caccgccgca aacctttttg tgttttcatc gggcagattt tgcatacttt
      781 ttacgagtta tttttgttgt ttttgtttca ggtattcttt gatgcgggat ttgtcttcgt
      841 tggcttggtc ggatttgtga taaatttcga tgaattcgat ttttggttcg gctggataaa
      901 acgcataaat aatcctgatg ccgctccgat ttccccgatt tttcaatgat ttgcaggcaa
      961 atttttttaa cttgcaaacg atgacattat caaagcaaaa cccccggatc gggaaaatgg
     1021 cccgcctatc aatcaaattt agatgataag cctccatggc ataaactttt gccttttcaa
     1081 tatcggcaag caaagaacca tagcgtttca acaagcgctt taaatctgcc tcaaacggac
     1141 cagtcaagct atagcgaatc ttcgccatcg tattttctta ccgaagtgtc atctgtccgg
     1201 taaaataccg cttcgtaatg catcggcttg cccaattcgc ccatcatcca aggaacatct
     1261 ttatgcgaca aatttgaaat ttcgttcgcg gtcatatccg acaagcgttc aagctcccaa
     1321 tcgatatgct ctttctcccg tccgtcaatc acgctcaagt ccggctcaac ttttggattg
     1381 atgatatatt ttgtttgcgg atattggtag aatttgcttt ttatcggctc aactttgttc
     1441 tccttgacca aatcccgcat aatcccttca aacatcaccg gcgtcggccc gaaattgttt
     1501 tttatataca gcgcgcccat caattgttcc tcgtattttt cataataatc aaaatcaata
     1561 aaatacagaa ttttatataa aaccgtcata ccgacattgg gtttgccgcc gatcttttta
     1621 agaatatata ataaaacttc gcgaaattta tcggcatatt cttgtggaat atcaatcctg
     1681 atatcgttgc ttttattttt ggatttttta tccggtttga tattgaccgt taaatttttt
     1741 tccgccaaaa aatcatccag cgccaaactg aaaatatccg ccattttttt ggcctcggtc
     1801 acagtcaggc cacgctggcc ttgttcgatt tgcgcatagg tcggccgcga aacaccgatt
     1861 tgggccgcaa gatattcctg ggtcagctga ttcttttccc tcagtttttt gatataattt
     1921 ggattcatcg tgttgattat tgttgtctta attttaattt atgtaaaata ttttgtcaag
     1981 cactatgtaa aaaatattaa caatttacga agcctcattg ccaaccgcta aaaaatccct
     2041 ggcaaaaggg atttttgcta aaagggtccg acccttttga aaaacatatc aggggcggca
     2101 gttgatgtcc cgcaaacagc catcttcgcc atattttacc gggtcataat tgggctggtt
     2161 gtttttgcgg caggcgagat atttttccag aagtttttga tcaatcgcgc aatccggatt
     2221 ttgcgcccgc gcaatcgcct ccttggcccg atcaatttcg gacacgcacc gatactcctc
     2281 aacgcatccg tttgaatcgt ttttgggctc aatctgcccg gcgcaccggc tcctctcggc
     2341 cgatgccggt tcggcgacat aggggcattt gaccgcctcc aaacattgcg gcgtccaggc
     2401 aacgccgctc tcctccttcg ccgcattctt caattgccat tgcggcccct gttcattctc
     2461 aaattccata tacaccataa acggcagatt gacctcgttg atcttttttg ccacgcagac
     2521 attgaaatac ccgtccatat tcgtggtctt gccgacaatc ttgaatttgg tgcagggaat
     2581 cgccgcggcg gcatcctcgc cctgatagat tattgaataa tttcccttgg accggccaaa
     2641 aatcacgtct tttaaaatca ccggagcacc cgagcgccaa tattcttcat cgttgaagtt
     2701 cttgcccgcg gcgataaaca attcgctcac gaacagcggc acgttcatct cgttaaaata
     2761 cggaaccgcg tcgtcaaacg ccaagcttcc ttcgttggtc caggtgctga ggcccaattc
     2821 gccgttatct tcataggcgg ccagctttgc atattggttg ttaaaccggc cgtccgatga
     2881 caccagcttc gccacgccca aataagcggc gccgcgagcg catgtttgtt ttttttcaaa
     2941 aaaataagaa agggtaatgt tttgcccggg accgaaattc ttcaattcgt aatccaggcg
     3001 caatggcgca atggtttgcg gctccggcgc cgcaggcggc gtttcatctc cgtcattaga
     3061 caccgacaac ggcatgttct cggacagcgg cgccgctttt cgcgccgaca tccaaagaaa
     3121 acccgcggcg gcggcaatca ccagcaaaat aacggcaagc aaaatcaaaa tttttggctt
     3181 catcttttta attttcagag ggtctaaccc tctgaatgat ttaatgctta cagttttttg
     3241 ccgcattcgc cgcaaaaggc ggtgccggcg ggatttttgg cgccgcattt ttcgcaaact
     3301 gccattgcca gcgactggcc gcaattattg cagaacttgc cggtgccggc gggcttgccg
     3361 cacttggggc acagcgtttg tttggcctca atctcgccgg tgaacacttt cgcggcggcg
     3421 gctttggcgt caatatcggc cgcctttttc ttggcccgcg ccgacgcaat ctggacgttc
     3481 tcgcgcggcg cgcactgggt gcaaagattt tcctgttcgt tccaatcgtt ttcgcacacc
     3541 cattgggtgc actggggaca gcgatggaaa tgcgccttgg cctcggattg cgcctgttca
     3601 aacgctttgt cgtgttcttt ttgccattcc gggctcatgc cgttgaatct ttcgccgatc
     3661 gcgtccatcc cccgatccac gccgtaaccg acattatatt tgccggcaat ctgcgccgcc
     3721 gccccgatca ggccgccgat gcctttcaac aatttgcctt tgttataggt tttggattca
     3781 atgaattttg ttttgtagcc ctcgttgcag atcgcgcaaa aaaacgtgaa ttggaatccg
     3841 gcttcggtgc tgttgtccgc aaaattatcc gtgaacggtt gtaatgccat attgattatt
     3901 gattattgat tattgattat tgattattga ttggaattat ttttgcgccg aacctttcaa
     3961 aggctggccg cacttgatgc atttatcgcc cagcggcggc tgttcaaatt ggcacaccgg
     4021 acaggcgacc accagttttt tgccgcaatc ttcgcaatat ttgccaaaga atgtcacctt
     4081 gccgcaaaac ggacaaacaa tgcgcaagtt caagccacag tgggcgcaag ctttccattt
     4141 cttatcaatc ggatcgtggc atttgggaca tctgtccggc cccagcggcc cttctttgcc
     4201 gcaaaacggg caaaccgcgg cgtccggaga aaccatctgc tcgcagtatt tgcagggatg
     4261 cttgtagccg accgcgggct tgtccgcctc tttggacccg ccgataaatt ccggcccctt
     4321 tttttcttcg ggctcgccgc cgtcagtttt tttttgagaa tcgcccgaaa atgccaggta
     4381 ggcgggaatt gcgataccga ttaccggtac cagcgtcaac gcgccgaccc agctttcttt
     4441 gccggtaaat tcggcaatct tcatccaaac gatgaccgcc gcaacgatat taatcaccgg
     4501 cacaaacagc aagaaaaacc accaccaagg cttttgcgcg attttcaaca ttaaaaacgc
     4561 gttagcgatc ggaacccacg ccagccacgc gggcgcggtg ttggttcttt tggcaatctt
     4621 ttgcagacaa accgcggaat aaatataaaa tatcagtgaa aaaaacaaaa tgacgcccaa
     4681 cacgatcaga ttgccgatga taaacgccgt tgaaattccc ggcccgccga aaagcatatc
     4741 aacgccgcca gcttgttcat gggatagcat tgcgttgccg tcatcatcat agaaaatttc
     4801 accgtcgccg ttttggccga ccataccgcc ggcgccgata cccccgcttc cagttcccgt
     4861 gtcaccgccg ggcggcacaa cgtcactgcc atcatcaggc acggcgattt ccgcgccccc
     4921 gccaaccgcc gcgccgatac ccagatcata cgaagccaac cgctctccgc aatattcgtt
     4981 ataacaagct accgccagat acacttcccc gcccttttga gccgtgaatg gaagatttat
     5041 gatttgcccc ggattgtcca cggaaacttc atcaatcagc tttctttgcg tgtcatagat
     5101 cgccagcaaa attgacgcct ccaacgatgg cacggcattg atgtttagac tttgcccgga
     5161 cgaaacattg aatttgtaaa aatcggcaag atcgtcgccg ccgctttcaa aagaatcgcc
     5221 gccggccaaa taacctttat gattgcccgg cgatatcgcc atcgcgccgg caatatcgcc
     5281 gccggcatcg gtcttgcttt ccgcgtcgta gcgatcggcg gttttgaatg taaccacata
     5341 ggagcccccg tcccaaatac catcgttata agtctccaaa taatacttac tgcccggcgc
     5401 acccgaaccc gataaatagg aaaattcaaa tgtatcgccg gtgccgccaa aatcattgcg
     5461 caactgccgt ctctcggaat catatagatt aaatccgaca ttggtgccat aattactgcc
     5521 cgcggcctga ttgaatttaa cactcacctg cgcctcctgt ccggaatcaa gattgaaata
     5581 aaagaaatcg cgatggtctt cgggaagcgc ggcaacctca taaactcccg gcgcgaccaa
     5641 taccgcggaa tcaaaatctc cgccgccggt aatcgcatcc gccgcaaaca ccgacatccc
     5701 ggcaagcgac aaacaacccg ccagtattgc gccacaaact gtttttttgt tcttcatatt
     5761 ttgctttgaa tcgcgaaact ctttacctct ttggatttgg gggttttaca attcaagata
     5821 attatatttc aaaaaaattt gcgaattatt ccggcacgca acaggttttg gccgccggat
     5881 ctttgcattt gctcatcccc aaaaacccgc aggcatactc cttttcgtag gaaccgcaat
     5941 cagccgcgcc gatgctttta tagccaacag gacaagtgcc gctttcctga caggttcccc
     6001 gatcgcattt ttccccgccg acagccatac aacatctggt ttgcttttta tcgcatttct
     6061 tgcaaaaacc ccagcaggcg gttttgcatt ttttttcctt gccgcagttg gcaatgccga
     6121 catccaccgt tccggagggg cattcgtcat aggtgcaaat tccgccactg caatcggtta
     6181 ccggctcggg atattcgcaa cagattgtgc tttgatattc gcaacatttc ttcaggccga
     6241 aaaatccgca cggcaggcaa gtccgcgccg gcgcgcaatc cgccgttccc ttggcaaaac
     6301 tgcccgccgc gcaagcgccg gcggcgcaat agccgttgca ttttgccggc gcggggagcg
     6361 gcgcgtcggt tttgggaaca cagcaaacat ttttggtgta atcgcaacag gtaaacaggt
     6421 tgaacaggcc gcacttatag caattttcct cctgcacgca ggaatctgtg cctttatttt
     6481 cgtatccttc gggacaaacc gacgatttca tacagcttcc ggcacaggtc ccggacgcct
     6541 cgggtttgcc aaacactttc tggcaacaga catttttttg gccgtccttt ttggggcacc
     6601 cgccagcgcc aaaatccatg gtgcccgccg gacaattttc caggcactgt ccttcgcaca
     6661 attgcttttg ggattcttcc cgcgccagac agcggccggc aaaacagccg tattcgcatc
     6721 tatgctccac gtccgccggt ttcccgtccc ggcaaatttg ctcaacgaca aaatctccgc
     6781 ggcaagcatc cgcataccgg cacggctcgc aaacatagtg ctcattgccg gcccgcgcgg
     6841 tttctccgta ataataaact tcgccttccc gatcgggatc gtcgccgtcg gcgtccatac
     6901 acggggattt aatctcgccg tccggcgtgg tgttgaaaac acagccgttg gcttccaatt
     6961 cggcctttct tgcctcgtat ttcttaatcc atccgcccgc gggatcggat tccgcggcgt
     7021 caatttcctc catatacctc tccatattct gataggtgaa cgcggcatat tccggcgccg
     7081 tgacatcgta aggatctttg agatgattga aaacttcgtc ggtgaccatt gtccccttat
     7141 ggcaaaccgc caaacgcgcg gtgatttcaa cgtagatttc atcggtcatc ttgcccgtcg
     7201 gcgctttcat cagcgcctca atcgcctggc cgtatccggt catatctaaa atttcatcgc
     7261 cttggccgta aagattttct ccggattccg ccgccgcgcc attgccggcc gaattgtcag
     7321 tcggttgcgc caggaccagc ggcaaaacgg caaacaggcc gatcgccaat acggcgaata
     7381 tctttgcttt atgtctcatt ttggttttgc gcgttatttt gctccgggac ggccggcggc
     7441 tccaaatcat cgccatccgc ccgccatttg gcgccgtctt tggccgcggc attaaactcc
     7501 agcaatttct gcacgtcctt tcgccaaatt tccggcgacc ggtgcaatgc ccggccgatg
     7561 aaaatttccc gcaaaaaccg cagttcggga atttgcgcca gcgcaaactt atatttccaa
     7621 tatttgggat cttgcaattt ctcctccggc aaataaatcg gctcccgccg aaaattaatg
     7681 cgccggacgc atttttgcac cgccgccgcg gttttttgaa cctcccggtc aagatcgttt
     7741 tttttcgctt caaattccaa cggcgcaacc aaacggaaaa aataggttgc cttgcccgac
     7801 ccgccctcgc tggacgcctc catcgcgatt gcattattgt tttttagcgg caccaaaaac
     7861 caggcataat cgccggacaa acttcccatc gccccgcgct taacgcccgc gtaaatctcg
     7921 ccgccgccgg ccaaagattt caaataatca tattcttctt tcatccccgc ggcttgcaga
     7981 aagtcctcaa ttttttccca cgttcccggc gccagcgaat ccacccgcga tttggcagca
     8041 caacgtccgt cgcgcatcac ctcggccaac tgccgcaccc ccagcgaatc cagcccggga
     8101 atcatttctt ttatctgcgt ttgcgcattc aacaagatcg ccgtcattcc ggcattaacg
     8161 gctttggcac agaaatcaaa atcctgcccc atttgcgaca gttcaatgtt ttcctcctcc
     8221 gcggacaggg cgattttaaa atcctgcgct ttcatctcgt caacatcgcc gtaaaatatt
     8281 ttaaacagtc cggattctcg cggcaaaacc aaaaacgaag tttcataaaa ttggatttgg
     8341 cattctccca aatctttgcc cgaccgcaac acccgggcgc gggcgcttcc cttgtccgcg
     8401 gcttctttca tcaggaaaat gtcaatcaaa accgcgttcc aggccgcgct aaaatgacgc
     8461 cagaaatctt caaactgata gcccagccgg gaaatttcca ccgccgcacc cgacttcaaa
     8521 ttcatcagga cacgataatc gtccgcggcg gcttgatcaa tttcacggat gtcaatgttt
     8581 agcgccttgc cattgtccgg caaaaaagaa atctcttctt ctttcaaaat tattttggcg
     8641 gcgccgcgct cctgttccgc gccagcggcg gtcaaaatcg cgtagtcccc ggcggtttta
     8701 aacaaaacct tatttttgac ttccgcgtca gcggcgggca cttcccggtt agcgtcttcc
     8761 ggattggaat gatccatgtg attttgtttt taatctcggc agttgccggc ggcggcattt
     8821 attttttcca gatggctttg gccgctttcc agaaatattt ggcggcgatt aacaacaccg
     8881 cgccgtaaat tgcatattgc aaaatttcca gcaaaaatat cgccagattg acgctaatgc
     8941 tttggacaat ccggttcaac ccgccgaccg catttttcac cgccgtttgc cacgaatctt
     9001 tgacatcacg ccaatcaaag tatgaattgt cgcgggcgta aacgttaaaa taagtgtaat
     9061 caagcttgtc ggcctgcagg gattttgacc ggcgcaattg ctcaagctgg gcggcggtgt
     9121 tcagccgctc ctgcgtcaat tgcttgatgg tgttgatctt attgttaatg actttggaca
     9181 aagtttcggc gtcattctgt tcggtggcca gtcccgacac ttcgtcataa gcggcattcg
     9241 cgcggttcaa agtgtcgtca atcaccgcca atttcttttc caaaatatca atctggccgg
     9301 tgtaatcttc cagccgttcc tgaatcgtgt aaatattttc ggtaacatct ttggggccca
     9361 ggcctttgag aaagcccaat atctcttccg ccttggcgcg ttggaccttg aaagtgtagc
     9421 ggcaatattc gtcggactca acggcgtttt caaagatcac ttccggccgc ggcttgagcc
     9481 cgcccacggc agcgcaggtt ttttcaagat cgcggctttc ataattaacg cggtattcgg
     9541 ccgtttcata attttcggcg gcatcgcccg cagtgccgcc gccatcatcg gcaaggctcg
     9601 ccgcgtttct ggcggaaaaa agaccggaga ttccgccgcc gctctccatc ggtaattcca
     9661 ttgatgaagc aagcggaacg gaattatact tttcggaaaa agcataatcg tccccgtatc
     9721 cggatgattt gtattcggat gacggataat atcccggcat tgccggcaga acggccggca
     9781 atgccgtcct cggaagattg attaccaaat aaatacccgc caatcccaaa accgccagtc
     9841 cggcgacttt ggccggtttg ttatttttaa aaaattcgcc gatctttttc aaaaaactcg
     9901 tctttgtttt tttctgttcc atttttttgc cggcagtctg caattatcgg acaaagcggc
     9961 ggccttgatt ttgcccccgc cattttaccc cgacgcagtt acggaacacc agcgttttat
    10021 ttgcctttta attcatttta aacgccggag gaaatattat caag
//
LOCUS       DAOQWE010000018         9686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_7520_length_9686_cov_5.117641, whole genome shotgun sequence.
ACCESSION   DAOQWE010000018 DAOQWE010000000
VERSION     DAOQWE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7520_length_9686_cov_5.117641"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..1974
                     /locus_tag="PKK37_01270"
     CDS             391..1974
                     /locus_tag="PKK37_01270"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNR81044.1"
                     /translation="MATKESRIDITKEQERAELHRAIWQIANDLRGSVDGWDFKQYVL
                     GMLFYRFISENLTNYINADERRAGKKDFDYTALSDEEAEFGRADTVKEKGFYILPSEL
                     FVNVRKNARNDANLNETLTAVFRNIENSAKGANSEDDIKGLFDDLDVNSNKLGGTVEK
                     RNQKLAKLFESIGDLRLGNYTDNTIDAFGDAYEFLMTMYASNAGKSGGEFYTPQEVSE
                     LLAEITTVGKKEVNKVYDPACGSGSLLLKFAKVLGKENVRLGFFGQEINLTTYNLCRI
                     NMFLHDINYNHFDIAHGDTLIDPKHWDDEPFDAIVSNPPYSIHWEGDANPLLINDPRF
                     SPAGVLAPKSKADLAFTMHMLAWLSTNGTAAIVEFPGVLYRGGAEGKIRKYLVDNNYV
                     DAVIQLPPDLFFGTTIATCVIVLKKSKKDNKTLFIDASAEFVRGGNKNKLSETNRKKI
                     LAAFIARQDAEYFAKLVDNKTIAENDYNIAVSSYVVGEDTREVVDITELNKKIAKIVA
                     RQNELRAAIDEIVADIEGK"
     gene            1976..3004
                     /locus_tag="PKK37_01275"
     CDS             1976..3004
                     /locus_tag="PKK37_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HNR81045.1"
                     /translation="MENIGPKNQIIIYKGEDGKSHVEVMFAGETAWLTQAQLVDLFQS
                     SKANISEHIKHIFEEKELDRDSVVRKFRTTASDGKNYEIEHYNLDMVISLGYRVKSHT
                     ATRFRIWATARLKEYIIKGFTMDDERLKENGGGKYWYELLDRIRDIRSSEKVLYRQVL
                     DLYATSIDYNPKALESIKFFKIVQNKLHYAAHGNTAAEVIARRANAGKPFMGLLTFSN
                     GGVSKKDIAIAKNYLNEEELKILNNIVSAFFDLAEVRVMKHEPMYMKDWIAQLDKLIG
                     IFDKKILTESGSVSHTAALKKAATEYKKYQAKTISEVEKSYLETIKTIQKTIEKKVRE
                     TKQTPRDN"
     gene            3048..3965
                     /locus_tag="PKK37_01280"
     CDS             3048..3965
                     /locus_tag="PKK37_01280"
                     /inference="COORDINATES: protein motif:HMM:NF037056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81046.1"
                     /translation="MDQINEQRKELIKALSNIDFDVMRNNLPNLDKINQAIEKIKMPD
                     SVFKEINKLVRTRENFVLSLQPSFEQQRVLTKSVKQFEEFSKGIMSTGIFLELSKIQK
                     LNIELFTSSLTNVEFPILNILGKDLYFSELALQPELPEKNEESEKLMEADLFIGLETE
                     RKLRMINPVFAKMYKGAVEASVGNNADRARHVAISLRELFTKLLNKLCPTKQVIVWID
                     DLNNKPKDYLIAGKPSRKAQINYYYRDEKDDFLEKFVNNKILLLESLINTSTSVHENL
                     DKISSDQLVNLIRSARYLIDDLLIKYDGN"
     gene            3983..4633
                     /locus_tag="PKK37_01285"
     CDS             3983..4633
                     /locus_tag="PKK37_01285"
                     /inference="COORDINATES: protein motif:HMM:NF025713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HNR81047.1"
                     /translation="MKLLAEFILNNLTNYPGWAEYNDVVLDFISSYKQKRPDMCVEGC
                     KSLIEGIAKFLYLNLDIDNQNLVKWKDWTLSQKFKKAIEMLNIDDYEKEFLMRNNSLV
                     QKLGEIRNERGDISHGQSYPKNTYSDEDFAKFIASWTEGLCYFLLSSYVYLKRKQQDD
                     ELSYTKEQFKEFDNYLDSLNPDIKYISYSKALREQDPLQYELLMDGFITNKENFSN"
     gene            4639..5844
                     /locus_tag="PKK37_01290"
     CDS             4639..5844
                     /locus_tag="PKK37_01290"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR81048.1"
                     /translation="MDRNQSKIEKLIAGLCPNGVKFKELGELGMFYGGLSGKDKDDFG
                     DGNAKFVTYMNIFSNIEVNTDINTFVKVIKDENQNKIEYGDVLFTGSSETPNECGMSS
                     VLTKKISEPLYLNSFCFGFRFHDANLLLPEFSKYLFRDEQMRKQIAKTASGVTRFNVS
                     KKRFAKIKIPLPPVAIQEEIVKILDGFTELEAELEAELEARKKQYEYYREELLMFEDD
                     VAIKRISEICQINRGRVMSKDYLKNNAGEYPVYSSQTADNGIFGRINTYDYDGEYLTW
                     TTDGAYAGSIFYRNGKFSITNVCGLLKAKSGDINMKFLSYILATVSKNYVSSGMGNPK
                     IMSNVMEKIKIPVPSIPEQERIVKTLDKFDALVNDISIGLPAEILARRQQYEYYRGKL
                     LNFKEYAKQ"
     gene            5831..9178
                     /locus_tag="PKK37_01295"
     CDS             5831..9178
                     /locus_tag="PKK37_01295"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNR81049.1"
                     /translation="MPSNKYNLVAESSHLPAPRPQRGEFCVYVLCCADGSFYIGQTDD
                     FENRLQDHAEGRGAKWTKNHLPFEPIHWEIFTSREEAVKREHDLKTGFGRKWLKRNYN
                     NYNLAARQAGSTVVAEYKPQYCDRVADYQGEAALERAFIEQLETQAYEYIKIGSEADL
                     ILNLRRQLEKLNDFIFTDAEWERFFKSELANPNQSIEEKTATIQDDYIKNLMRDDGTV
                     KNVYLIKKDNIHDNSLQVINQYAIDDGARANRYDVTVLVNGLPLVHIELKRRGVALQE
                     AFNQINRYQRESFWAQSGLFEYVQLFVISNGTHTKYYSNTTRREHIKEANEGAAKSGR
                     RIGNSFEFTSWWADAANRPITDLMDFAKTFFAKHVILNVLTRYCVFTTDHLLLAMRPY
                     QIVATERILKKIEISTNYKKLGTVEAGGYIWHTTGSGKTLTSFKTAQLVSKLPYIDKV
                     IFVVDRKDLDYQTMREYDKFEKGAANSNTSTAVLKKQLENPNARIIITTIQKLDRFIS
                     RNAGHTVFDGHIVLIFDECHRSQFGDMHAAIVKAFKNYHLFGFTGTPIFAVNASSGGR
                     ADFKTTEQAFGEKLHTYTIVDAIADRNVLPFKVDYVSTVREAEDIEDKKVSDIDREAV
                     LAAPARLANIVGYIRDHFDQKTKRNSFYKLKDRRLAGFNSIFATASIEAAKKYYAEFK
                     KQLKDIPSDKKLKIALIYSFGANDEDADGMIDENSEDTSGLDVSSREFLDGAIADYNE
                     MFGTSYNTSSDKFQNYYKDVSLRVKNREIDILIVVNMFLTGFDAITLNTLWVDKNLRL
                     HGLLQAYSRTNRILNSVKTFGNIVCFRNLEKATNESIALFGDREAGGIVLLKAYDDYY
                     NGYQDGDKEVRGYKSFVEELLQKFPVGERIIGERNQKDFIKLYGSILRVRNILTAFDE
                     FAGNEILTARDIQDYHSAYIDLYNEFRNSEERGKENVNDDLIFEMELIKQIEINIDYI
                     LGLIKKYHQDHIKNREILLDINKAIDSSVELRNKKELINQFIATLDIHSAVDDDWQKF
                     VEKKKIEELDKIIAEEKLDRDAAYKFVQNAFRDGGVATTGTAIAKVLPPVSRFSPTGE
                     RTKKRESVLSKLTSFFEKFFDISGKKL"
     gene            9338..9583
                     /locus_tag="PKK37_01300"
     CDS             9338..9583
                     /locus_tag="PKK37_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81050.1"
                     /translation="MENQKSQIVIYQSLGGGNRIHVRIEDENVWLSQKLIAELFDVDV
                     RTVNEHLKNIFLEQELDENSVIRNFRITAADGKKYEF"
BASE COUNT         3152 a         1704 c         2198 g         2632 t
ORIGIN      
        1 aaactatcgt taatcaaaaa ccagagttga cgctatttct acatttctat tttgaaataa
       61 tcgcgcaata aattttactg aaataatcca aaatttgatg cgccggcaaa accgaaaatt
      121 tttaatgtta tatttatgtt atatttatgt tatatttaga catagcattt tggcgcgcca
      181 aaaaatccgc ccgccgcata taccgcattt tagccgaaaa gatgggggta aaggggagaa
      241 tttgttttat tttatgcgaa gtccgctggc tttgccaaaa ctggctttgg caaaaattgg
      301 ctttggcaaa aattgaaaaa agggtcaaaa aaggttaaat tataatgaga gatatatata
      361 ctttatacgg cgtcaaacaa ataaaatttt atggctacca aagaatcacg cattgatata
      421 actaaagagc aggagcgggc ggaattgcac cgggcgattt ggcagattgc caatgatttg
      481 agaggaagtg tggatggctg ggatttcaaa cagtatgttt tggggatgct tttttatcgt
      541 tttatcagtg aaaatttaac caattatatc aatgccgatg aacgccgcgc gggcaaaaaa
      601 gattttgact atactgcgct ttccgatgaa gaggctgaat ttggccgggc cgatacggta
      661 aaggaaaaag gtttttatat tttgcccagc gagctttttg taaatgtgcg caaaaacgcg
      721 cgcaatgacg ccaatcttaa cgaaacctta accgctgtct tccgcaatat tgaaaattcg
      781 gcaaaaggcg cgaacagcga agatgacatt aaaggattat ttgatgatct tgatgtgaat
      841 tccaataagc tgggcggcac ggtggaaaaa cggaatcaga aacttgcgaa actttttgaa
      901 tctatcggcg acctacgcct gggcaactac accgataaca ccattgatgc cttcggcgac
      961 gcttacgagt ttttaatgac catgtatgcc tcaaatgccg gcaagtccgg gggcgagttc
     1021 tacactcctc aagaagtcag cgaacttctg gcggaaatca ccacggttgg taaaaaggaa
     1081 gtgaataagg tctatgatcc cgcttgcggt tcgggttcgc tgctcctcaa atttgccaaa
     1141 gttttgggca aagaaaatgt gcggctgggt ttctttgggc aggaaatcaa ccttaccacc
     1201 tacaatcttt gccggatcaa tatgtttttg cacgatatca attataacca ttttgatatt
     1261 gctcacggcg atacgctcat agacccgaaa cactgggacg atgagccgtt tgacgccatt
     1321 gtttccaatc cgccgtattc cattcattgg gaaggcgacg caaatccgct cctgatcaac
     1381 gatccgcgtt tttctccggc cggagtgctg gcgccaaaga gcaaggccga tctcgcgttc
     1441 acgatgcata tgctcgcgtg gctttccaca aacggaaccg cggcgatcgt tgaatttccc
     1501 ggcgtgctct atcgcggcgg cgcggagggt aaaataagaa agtacctggt agacaacaat
     1561 tatgttgacg cggtgatcca gcttccgccc gacctgtttt ttggcacgac catcgccact
     1621 tgcgttatcg tgctcaaaaa gagcaaaaaa gacaacaaaa cgcttttcat tgacgcttcc
     1681 gctgagtttg tccgcggcgg caacaaaaac aaattgagcg aaacgaatcg caaaaagatt
     1741 ttggccgctt tcatcgcccg ccaagacgcg gaatactttg ccaagctcgt tgataacaaa
     1801 accattgccg aaaacgacta taatatcgcc gtttcaagtt atgtggtggg cgaagatacg
     1861 cgcgaggtcg tagacatcac cgaactcaac aaaaaaatcg ccaagattgt cgcccggcaa
     1921 aacgaactcc gcgccgcgat tgatgagatc gtggcggata ttgagggaaa ataatatgga
     1981 aaatattgga ccaaaaaatc aaataatcat ttataaaggc gaagatggaa aatctcatgt
     2041 agaggtgatg tttgcaggcg aaaccgcttg gcttacgcaa gcacagttag tggatttgtt
     2101 tcagtcaagc aaggcgaata tcagcgagca tatcaagcat atatttgagg aaaaagaact
     2161 ggatcgggat tcagttgttc ggaaattccg aacaactgct tcagacggaa aaaattacga
     2221 aattgagcac tataatcttg atatggtcat ttctttggga tacagagtga aatcccatac
     2281 cgctacaagg tttcgcattt gggcgaccgc gcgccttaaa gaatatatta tcaaaggatt
     2341 tacgatggac gatgaacggc tcaaagagaa tggcggggga aaatattggt atgagctcct
     2401 tgatcgcatt cgcgacattc gcagcagtga aaaagtttta taccgccaag tgcttgacct
     2461 gtatgccacg agcattgatt acaatcccaa ggccttggaa tccataaaat ttttcaagat
     2521 tgtgcaaaat aaactgcatt acgccgcgca tggcaacacc gccgccgagg tcattgcccg
     2581 gcgagccaat gccggcaagc cgtttatggg attgctaact ttttccaacg gaggcgtgag
     2641 caaaaaagat atcgccatcg cgaaaaatta tctcaacgag gaagaactga aaatactgaa
     2701 caatatcgtt tccgcttttt ttgatttggc ggaagtgaga gtgatgaaac acgaaccgat
     2761 gtatatgaag gattggattg cccaacttga taaattgatc ggcatttttg ataaaaaaat
     2821 cctgacggaa tccggttcgg tgagccatac cgcggcactg aaaaaagcgg caacagaata
     2881 caaaaaatac caagccaaaa ccatcagcga agtggaaaaa tcatatttgg aaacaatcaa
     2941 aaccattcaa aaaacaattg agaaaaaagt ccgcgaaaca aaacaaactc cgcgcgacaa
     3001 ttgatgaaat cgtggcggat attgagggaa aataaacttt attaaatatg gatcaaataa
     3061 atgaacagcg aaaagaacta ataaaggcat tatcaaatat tgattttgat gttatgcgta
     3121 ataatttgcc aaacttggat aagattaatc aggcaattga aaaaataaaa atgccggata
     3181 gcgtatttaa ggaaatcaat aaactagtcc gaacgcgaga gaattttgtg ctatctttgc
     3241 aaccgtcatt tgagcaacaa cgggttttaa caaaatcagt aaagcagttt gaagaatttt
     3301 ctaaaggtat aatgtctaca gggatttttt tggaattaag taaaatccag aaattaaata
     3361 tagaattatt taccagcagt ttgaccaatg ttgaatttcc gattttaaat atattaggaa
     3421 aagatttata tttttctgaa ctcgctctcc agccggagtt accagaaaaa aatgaggaaa
     3481 gcgagaaatt gatggaggct gatttattca ttggattaga aacagaaaga aaactgagaa
     3541 tgattaaccc tgtttttgca aaaatgtata aaggagcggt ggaagcatct gtaggaaata
     3601 atgccgatag agcaaggcat gttgcaattt ctctacgtga attatttaca aaattactaa
     3661 ataaactatg tccgacaaag caagttattg tttggattga tgatctaaat aataaaccta
     3721 aagattactt aatagcgggt aagccaagta gaaaagctca gataaattat tactatagag
     3781 atgaaaaaga tgacttttta gaaaaatttg taaataataa aatcttattg cttgaaagtc
     3841 taataaatac ctcaacatca gtccatgaaa atttagataa aatatcgtcg gatcaactag
     3901 ttaatttaat tcgatccgca cgatatttaa ttgatgattt attgataaaa tatgacggta
     3961 attgattttt taaatcaatc ttatgaaatt gcttgcggag tttattttga acaatttaac
     4021 aaattatcct gggtgggccg agtataatga tgttgttttg gattttatat catcttacaa
     4081 acaaaaacgg ccagacatgt gtgttgaggg ttgtaaaagt ttgattgaag ggatagcaaa
     4141 gtttctatat ttaaatttgg atattgataa tcaaaatttg gtaaaatgga aagattggac
     4201 cttatcacaa aaattcaaga aagcaattga aatgttaaat atagacgatt acgagaaaga
     4261 gtttctcatg agaaataatt ctttggttca aaaattagga gagattcgca atgaaagggg
     4321 tgatataagc catggacaat catatcccaa gaatacttat agtgatgagg atttcgcaaa
     4381 attcatagct tcatggaccg agggattgtg ttatttctta ttatcaagtt atgtttatct
     4441 taaacgaaaa cagcaggacg atgagctgtc ttacaccaag gaacaattta aagagtttga
     4501 caattattta gatagtctga acccagatat taaatatatt tcttatagta aagctctcag
     4561 agaacaagat cctttgcaat atgaattgtt aatggacgga tttataacca ataaagaaaa
     4621 ttttagtaat taaattttat ggacagaaat caatcaaaaa ttgaaaaact gatcgctggg
     4681 ctctgtccaa atggtgttaa gtttaaagag ttgggagagc ttggcatgtt ttatggcgga
     4741 ttatctggaa aggataaaga tgatttcggt gatggtaacg caaaatttgt tacttatatg
     4801 aatatctttt caaatattga agtaaatacc gatattaata cttttgtaaa agtaatcaag
     4861 gacgaaaatc aaaacaaaat cgaatatggc gatgtattgt ttacaggatc gtcagaaaca
     4921 ccgaatgagt gtggaatgtc atctgtttta accaagaaga ttagtgagcc cttatatctc
     4981 aatagtttct gttttggttt tcgttttcat gatgccaatt tgctgttacc agaattttct
     5041 aaatatttat ttagagatga acagatgaga aaacagatag caaaaactgc cagcggagta
     5101 acgaggttta atgtgtcaaa aaagcgattt gcaaaaatta aaatcccgct cccgcccgtt
     5161 gcgatccaag aagaaattgt caaaattctt gatggtttta cagagctgga agcagagctg
     5221 gaagcagagc tggaagcaag gaagaagcaa tatgagtatt atagggaaga actgttaatg
     5281 tttgaggatg atgtggcaat caaaaggatt agtgaaatct gccaaatcaa tcgcgggaga
     5341 gtcatgtcaa aagactattt gaaaaataat gccggcgagt atccagtata ttcatcacag
     5401 acggcagata acggtatttt tggacgaata aatacttacg attatgacgg tgagtattta
     5461 acttggacaa ctgatggcgc gtatgctggt tcaatttttt atcgtaacgg aaagttcagc
     5521 attacgaatg tttgcggact tttgaaagcg aaatctggcg atataaacat gaagtttctt
     5581 tcatatattt tggcaacagt ttctaaaaat tatgtaagtt ctgggatggg caacccaaaa
     5641 atcatgagta atgtaatgga aaaaattaaa attcctgttc catctattcc agaacaggaa
     5701 cgcattgtga agactttaga taaatttgat gcgcttgtga atgatatttc aattggtttg
     5761 cccgcggaaa ttttggctcg caggcagcaa tatgaatatt accgggggaa acttttaaac
     5821 tttaaggaat atgccaagca ataaatacaa tttagttgcg gagagttcgc acctgcctgc
     5881 gccgcgaccg caaagaggag aattttgcgt atatgtttta tgttgcgcag atggttcttt
     5941 ttatattggt caaaccgatg attttgaaaa taggttgcaa gatcacgcag aaggcagggg
     6001 agctaaatgg accaagaacc atctgccttt tgagccaatt cattgggaga tttttacttc
     6061 gcgagaagag gcggtaaaac gagagcatga tttaaagaca ggttttggaa gaaaatggct
     6121 caaaagaaat tataataatt ataaccttgc ggcgcggcag gcaggaagca cggtggtggc
     6181 ggaatataaa ccgcaatatt gtgatcgggt ggcggattat cagggcgagg cggcgctgga
     6241 gcgggcgttt atagaacagc tggaaacgca ggcgtatgag tatattaaaa tcggttccga
     6301 ggcggatttg attttgaatt tgcggcggca gttggaaaaa ctcaatgatt ttatttttac
     6361 cgatgccgaa tgggaaagat ttttcaagtc cgaattggcg aatcccaatc aaagcattga
     6421 agaaaagacc gccacaattc aggatgatta tatcaagaat ttgatgcgcg atgatgggac
     6481 ggtgaaaaat gtttatttga tcaagaaaga taacattcac gacaacagct tgcaggttat
     6541 caatcaatac gcgattgatg atggcgcgcg cgccaatcgc tatgatgtga cggtgctggt
     6601 aaatggtttg cctttggttc atattgagct caaacgccgc ggcgtggctt tgcaggaagc
     6661 gttcaatcag atcaaccgct accagcgcga aagtttttgg gcgcaaagcg gcctttttga
     6721 atatgtccag ctttttgtca tttccaacgg cacgcatacc aagtattaca gcaataccac
     6781 gcgccgggag catatcaaag aagctaatga aggcgcggcc aagagcggcc ggcgcatcgg
     6841 caatagtttt gaatttacca gctggtgggc cgacgcggct aaccgtccga ttacggattt
     6901 aatggatttt gccaaaactt tttttgccaa gcatgtgatc ttgaatgttc tgacccgtta
     6961 ttgtgtgttt accaccgatc atttgctttt ggcgatgcgg ccctatcaga tcgtagcgac
     7021 cgagcggata ttgaaaaaaa ttgaaatcag caccaattat aaaaaacttg gcactgtgga
     7081 agcagggggt tacatttggc atactaccgg atcgggcaaa actttaacca gtttcaaaac
     7141 cgcgcagttg gtcagtaaat taccgtatat agacaaagtg atttttgttg ttgaccgtaa
     7201 ggatttggat tatcaaacca tgcgagaata tgacaaattt gagaaaggcg cggccaacag
     7261 caacaccagc accgcggttt tgaaaaaaca gcttgaaaat ccgaacgcgc gcattatcat
     7321 caccaccatc caaaaattgg atcggtttat ttctcgcaat gccgggcaca ctgtttttga
     7381 cggccatatt gtgttgatat ttgacgagtg ccatcgctcg cagtttggcg atatgcacgc
     7441 ggccattgtc aaagcgttta aaaattatca tctttttggg ttcaccggca cgccgatttt
     7501 tgccgttaat gcttcttcgg gcgggcgggc ggatttcaag acgaccgaac aggcattcgg
     7561 cgagaaattg cacacctata ccatcgtgga tgcgattgcc gaccggaatg ttttgccgtt
     7621 taaagttgat tatgtttcca cggtgcgcga agccgaagat attgaagata aaaaagtcag
     7681 cgacattgat cgcgaagcgg tgctggcggc gccggcacgg ttggcgaata tcgtcggtta
     7741 tattcgcgat cattttgacc aaaaaacaaa acggaacagt ttttataaat taaaagatcg
     7801 gcggcttgcc ggattcaatt ctatttttgc gactgcctca attgaagccg cgaaaaaata
     7861 ctatgccgaa tttaaaaagc aattaaagga tattccaagc gataaaaagc tcaaaatcgc
     7921 gttgatttac agctttggcg caaacgatga agacgccgac ggaatgattg acgaaaattc
     7981 ggaagatacg agcggattgg atgtaagctc cagagaattt ttagatggcg cgattgctga
     8041 ttataacgaa atgtttggca cttcttataa cacctcatcc gataaattcc aaaattatta
     8101 taaagatgta agtttgcggg tgaaaaaccg cgaaattgat attctcatcg tggttaatat
     8161 gttcttgacc ggctttgacg cgatcacgct caatacgttg tgggttgata aaaatttgcg
     8221 tttgcacggc cttttgcagg catactcgcg caccaatcgc attctaaaca gcgtgaaaac
     8281 atttggcaat atcgtttgtt tccgaaactt ggaaaaggcg actaatgaat ccatcgcgct
     8341 ttttggcgat agagaggcgg gcggcatcgt gcttttgaaa gcttatgatg attactataa
     8401 cggctatcaa gacggcgaca aggaagtgcg cggctacaag agttttgttg aagaactttt
     8461 gcaaaaattt ccggtgggcg agcggattat cggggaacga aatcaaaaag attttatcaa
     8521 actttacggt tcaattttgc gcgtgcgcaa cattctgaca gcttttgacg aatttgccgg
     8581 caatgaaatt ttaaccgcgc gcgatattca agattaccat agcgcatata ttgatcttta
     8641 taatgaattc cgcaatagcg aagaacgcgg caaagagaat gttaatgatg atttgatatt
     8701 tgaaatggag cttattaagc agatagaaat taacattgat tatatcttgg ggctgatcaa
     8761 aaaatatcac caagaccata ttaaaaaccg cgagattttg ctcgatatca ataaggcgat
     8821 agattccagc gttgaattgc gcaacaaaaa agagctcatc aaccaattta tcgcgacact
     8881 tgatatccat tcggcagtgg atgacgattg gcaaaaattt gttgaaaaga aaaaaattga
     8941 agaacttgat aaaatcatcg ccgaggaaaa attggatcgc gatgcggcat ataagtttgt
     9001 gcaaaacgct ttccgcgatg gcggtgttgc tacgaccggc accgcgatcg ccaaggtttt
     9061 accgcccgtg tcgcgttttt ctcccacggg agaacgcaca aagaaacgcg aaagtgttct
     9121 aagtaaacta acaagtttct ttgagaaatt ctttgatatt tccggcaaaa aattataatg
     9181 aaaaaatggc gccaactccg attttttaaa aattcgcggg agaatgatta ccgcttggga
     9241 tttggggaaa atcaaataaa attatggatt tagttagtat ttaaatacgg acttgaattg
     9301 tcggatggga ttatgggttt tgtggtaaaa tgagattatg gaaaatcaaa aatcgcaaat
     9361 tgtcatatat caatctttgg gtggcgggaa tagaatccat gtcaggattg aggatgaaaa
     9421 tgtttggctt tcgcaaaaat tgattgccga gctttttgat gttgatgtgc gcacagtcaa
     9481 cgaacatttg aaaaatatat ttttggagca agaattggat gaaaattcag ttatccggaa
     9541 tttccggata actgccgccg acggcaaaaa atacgaattt tgatcgcgaa gtaaaaaaaa
     9601 ttttagaaac agagaaaaag aaaggtggaa gcgtttgaga taaatgaaaa attgggcggg
     9661 tttatcaagg acgggaggcg gttggg
//
LOCUS       DAOQWE010000019         9381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_7821_length_9381_cov_6.709843, whole genome shotgun sequence.
ACCESSION   DAOQWE010000019 DAOQWE010000000
VERSION     DAOQWE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9381
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7821_length_9381_cov_6.709843"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1689
                     /gene="dnaB"
                     /locus_tag="PKK37_01305"
     CDS             301..1689
                     /gene="dnaB"
                     /locus_tag="PKK37_01305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNR81051.1"
                     /translation="MAGDNIPGKIPPQNLEIEQSVLSCLMLDHNAMSKVADVLSSDDF
                     YKHNHRTIYEVCRELFEKGDPIDFLSVSNRLKEKGELEAIGGSTYLTELVNLVPSFGH
                     VASYARIVRHKRVLRDLISVSRDIEMLGYDESEDLETVLDAAEKKIFNIAQKGISQEF
                     TAIKETLPDAFDRIERLSQGSGTRGLPTGFVDLDKMLSGLQKSDLVILAARPSLGKST
                     LALNIAANIAATEKIPVGVFSLEMSKDQMVDRLISAYSGVDLWKIRTGRLQTEDFENI
                     QRALGILAEMPLFIDDVGSPSVLQMKALSRRLQAENGLGLLVIDYLQLMEPLNRMASP
                     VQQVSENSRALKGLARELNIPILVLSQLSRAVEQRIPPVPKLSDLRESGAIEQDADVV
                     MFIYREDRYRENSEKKGIAEIIVAKHRNGPVGKQNLFFDERTVCFKNYTEGYEYQDQG
                     GQYAASEEQLGI"
     gene            complement(1731..2150)
                     /locus_tag="PKK37_01310"
     CDS             complement(1731..2150)
                     /locus_tag="PKK37_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR81052.1"
                     /translation="MTTINAIESKLKAIDEYYAQLMNYRQHSQESLKDDATLKGALER
                     YLYLICQSAIDLGEAVIAFKDFYRPASYADVFETLKEKNVIPIQLAQKMIKMTGFRNS
                     IIHDYQKFDFGIAYEVLMSGIDDIKEFESIIKKYLNL"
     gene            complement(2147..2557)
                     /locus_tag="PKK37_01315"
     CDS             complement(2147..2557)
                     /locus_tag="PKK37_01315"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR81053.1"
                     /translation="MKDDIENDLQKYRDLMKDLAREMPQIVLAYIFGSTATNTRGPMS
                     DYDFAVYLDEPDIKKRYDIKFSLMGKLSQALKTNKVDVAVINDTDNVYLKHAIISKGK
                     ILFEKEPHKVIVEPKILNEYFDFRMLIDKYQKIK"
     gene            complement(2671..3345)
                     /locus_tag="PKK37_01320"
     CDS             complement(2671..3345)
                     /locus_tag="PKK37_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81054.1"
                     /translation="MGRKKIILHIAGYTVDDMKAQRRRRVDIPSLRKKYAEWDIELRE
                     LRVWPFGLTKIPVFEAVEEAQLEVDEVYEENPNALIGLSGHSLGGIIAMITAYSMDVD
                     FLILEDVPFGGSPDWVLKFGKFDLTNEAVITGLRNNSEGLKFIDGALEEKKFWPPILE
                     IRGWFAIGGQLLFGSFPNTGFGKVARFPLLNHHQVWKHQKVVNEVIHFIQSVAITADT
                     SGSLEG"
     gene            3517..3993
                     /locus_tag="PKK37_01325"
     CDS             3517..3993
                     /locus_tag="PKK37_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81055.1"
                     /translation="MDKSSKKQPVTLESLAQTMAAGFSTLDKKIDSKFEVLDKKIDRK
                     FGILDKKIDTKFGILDKKIDTKFGILDKKIDTKFGILDKKIDAVDKKIDDTSEQLLIK
                     MQQGFLEQIERIECVERSVTELKAGQARIETNLAGAARNIDLMDLRAEVEILKRKV"
     gene            complement(4042..4359)
                     /gene="rplU"
                     /locus_tag="PKK37_01330"
     CDS             complement(4042..4359)
                     /gene="rplU"
                     /locus_tag="PKK37_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNR81056.1"
                     /translation="MLAVIKTGGKQYIVTPGQKLKVEKLAGEEGAAIKFEEVLLVEGD
                     DKKVSVGQPVVAGVAVSAKIVAQTKGPKVIAYKYKAKKRYHLKKGHRQQLTEVEITGV
                     ELK"
     gene            complement(4543..5589)
                     /locus_tag="PKK37_01335"
     CDS             complement(4543..5589)
                     /locus_tag="PKK37_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNR81057.1"
                     /translation="MDTITLLLFIAVIVALGMQTWFLLRKRKGEELPKNDAAMAMLQQ
                     QLSQVSQTLDAKLAESNRAMQEQFGQSFAVIKDVTQKLTELDSTNKQVVGFAQQLQSL
                     ENILKNPKQRGILGEYYLETVLKNVLPPQSFQMQYKFKNGEIVDAAVFVKDKIIPIDS
                     KFSLENYNKIINEKDETRKRELEKIFKADLKMRIDETSKYIRPGEGTMEFALMFIPSE
                     GIYYDLLINEVGAVKVNTRDLIEYAFTQKRVIIVSPTNFLAYLQTVLQGLRALQIEES
                     AKEIRQRVEMLEKHLCAYNDCLQRLGGHLATSVNTYNAAYKEFGKIDKDVLKITDNGR
                     DKLEVKQIDKPNLD"
     gene            complement(5667..6170)
                     /locus_tag="PKK37_01340"
     CDS             complement(5667..6170)
                     /locus_tag="PKK37_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR81058.1"
                     /translation="MDKQLKNYAFIDGNNLNLGVKSLGWKLDYLKFRRYLKEKYHIEV
                     AYIFLGYIASFQPLYSSLQKYGYVLVFKPTIPDDNGDIKGNIDANLVLQAMIDFPKYD
                     NGLIVTSDGDFYCLIDRLLENQKLEKVISPYRDKCSSLLRKSAKGKIVFMENLKKKLE
                     YKRKSSA"
     gene            6270..6497
                     /locus_tag="PKK37_01345"
     CDS             6270..6497
                     /locus_tag="PKK37_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81059.1"
                     /translation="MADLSNFGNGLMGAFLFLMIGTGALLTLTAENWDWYQPIILQIV
                     HLLGGDWIFVGVMMVIGFIIGWIMQELSPPL"
     gene            complement(6522..9374)
                     /gene="uvrA"
                     /locus_tag="PKK37_01350"
     CDS             complement(6522..9374)
                     /gene="uvrA"
                     /locus_tag="PKK37_01350"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNR81060.1"
                     /translation="MELSGVRVHNLKNISVKIPKNKLAVICGISGSGKSSLAFDTIFQ
                     EGQRRYLESLSSYARQFLGGLKKPDVDKISGLSPTIAVNQKTISSNPRSTVGTLTEIY
                     DYLRLLFAHIGVAHCPKCGRPVSAQTPDHIAEKIYQTALKKEVSVVAPAVAAKKGAHA
                     GVLEEIYRSGWPKVIIDGITYDAAEAKDKVLDRNKAHTIGVAVDVFDLRQFNEKPKNE
                     KKQISKSEREALAAKKKRQENFMKEEKERILAAVKKGLEIGKGKIIADRPFSTRFSCP
                     DCDISLPDIEPKIFSFNSPYGACKTCQGLGKIAKINESLILNPRLSLNQGAILPWFSL
                     GRWSLRTMGVPHQKWSLQNLAAAKSFSLDEPFGNLPEHIQTLVLYGDKSDPSYEGIIS
                     RMERAYRETTSEYLRGEITKYMSEIECSACGGARLSPEALSVKIAGSNIADVCRLPAK
                     DAIVFLENLKTELPEKQKEIARPLLAEICKRLSFLAEVGVDYINLGREATTLSVGENQ
                     RIRLACQLGSALSGVVYVLDEPTVGLHQRDVDRLLGALKKLKDLKNTVIVVEHDEQVI
                     QAADWVIEIGPAAGKHGGKLVFEGTPKQLLRAKTLTGKYLSGRMSISAGFKKREVSER
                     TLWLELAGAKQFTLKGTDLKIPLGKLTAVCGVSGSGKSTLVIETLAAALLRDLMRART
                     VPGKYESLAGTERINKAVLVDQSPIGKTPRSNPATYTGVFNYIRELYTRTRDSQIRGF
                     TPGHFSFNTQKGRCPVCAGEGWQKVEMYFLPDIYVECEACQGKRFTAEVLSAKYRDKS
                     IADVLEMSVDEAKNFFADIPPINDKMQTLSDIGLGYLQLGQSAPSLSGGEAQRVKLAT
                     ELAKRDTGRTIYILDEPTVGLHFDDAKKLLIILRRLVEKGNTVIVIEHNLDFIKEADW
                     CVEMGPDGGANGGQIIFTGPPEQLAKSNTWTAKFLK"
BASE COUNT         2384 a         2228 c         2113 g         2656 t
ORIGIN      
        1 ttgaaaaaat ttgaaagaaa aaggtggaaa aatccgctat aaatatacaa aaaattgtaa
       61 ttataacaag aatcaatata atagaaattt tttgatttct ttgccttttc cgccttatat
      121 tttcatctct tattctgcta agaacaggag acaatgaaca attgctgtta atgttttttc
      181 ctccttttcc ggcagttcca aatgccgggc ataagcaagt gtatagccca accgcttccg
      241 attgtcaatg gttgtgtttc ctctttggtt ttgctaggat ggacaatcaa gaaatccttt
      301 atggccggcg acaatattcc gggaaaaatc ccgccccaaa atttggaaat tgagcaatcg
      361 gtattgagtt gcctgatgct ggatcataac gcgatgagca aagtcgcgga tgttttgtcg
      421 tccgacgatt tttacaagca caatcatcgc acaatctatg aagtatgccg cgagttgttt
      481 gaaaaaggcg acccgattga ttttttatcg gtttccaatc gtttgaaaga aaagggcgaa
      541 ttggaagcaa tcggcggcag tacttacttg accgaactgg tcaatctggt gcccagcttc
      601 ggacacgtgg cgtcctatgc ccgaatcgtg cgccacaagc gggtcttgcg cgacttaatc
      661 tcggtgtcgc gcgatattga aatgcttggc tatgacgaaa gcgaggacct ggaaacggtg
      721 ctggatgccg cggaaaaaaa gattttcaat atcgcgcaaa aaggaatttc ccaggaattt
      781 accgccatca aagaaacctt gccggacgcg tttgaccgca ttgaacgatt gtcgcaagga
      841 agcggcacgc gcggtctgcc caccggtttt gttgatctgg acaaaatgct ttcgggtttg
      901 cagaaatccg atctggtgat tctggcggcg cggccgtcgt tgggtaagtc cacgctggcg
      961 ttgaatatcg ccgccaatat cgccgccacg gaaaaaattc ccgtgggcgt gttttcgctg
     1021 gaaatgtcaa aagaccaaat ggtggatcgc ttgatttcgg cctattcggg ggtggatttg
     1081 tggaaaatcc gcaccggccg actgcaaacc gaagactttg aaaatatcca gcgggcgctg
     1141 ggaattctgg cggaaatgcc gctgtttatt gatgatgtcg gctccccgtc cgttttgcag
     1201 atgaaagcgc tgtcgcgccg cttgcaagcg gaaaacggct tgggactatt ggtgattgac
     1261 tacctgcaat tgatggagcc gctcaaccgc atggccagcc ccgtccaaca agtttcggaa
     1321 aattctcgcg ccctgaaagg gttggcgcgg gaactcaata tccccatcct tgtcctatcc
     1381 cagctttccc gcgcggtgga acagcgcatc ccgccggtgc ccaagttgtc cgatctgcgg
     1441 gaatccggcg ccattgagca ggacgccgac gtggtaatgt ttatttatcg cgaagaccgt
     1501 tatcgcgaaa acagcgaaaa gaaaggcatt gccgaaatca ttgttgccaa gcaccgcaac
     1561 ggcccggtgg gcaagcaaaa cctgtttttt gacgaacgca ccgtgtgctt caaaaactac
     1621 accgaaggtt acgaatacca agatcaaggc ggccaatacg cggccagcga agaacaattg
     1681 ggaatataaa atcattaatc attgatcatt aataactaag cccaaaaaaa ttacaaattc
     1741 aggtattttt tgataatgga ttcaaattct ttgatatcat caattccgga cattaaaact
     1801 tcataggcaa tgccaaaatc aaatttctgg tagtcgtgga tgattgaatt tctaaaaccg
     1861 gtcattttaa tcattttttg cgccagctgg atgggaataa cattcttttc ttttaaagtt
     1921 tcaaaaacat cggcatagga agcggggcgg taaaaatcct tgaatgcaat caccgcttcg
     1981 cccaaatcaa tcgcggattg gcagattaaa tacaagtatc gctccaaagc gcctttgagg
     2041 gtggcgtcat ctttcaagct ttcttgcgaa tgttggcgat aattcatcaa ttgagcgtaa
     2101 tattcgtcaa tcgctttcaa tttggattca atggcgttaa ttgtggtcat tttatttttt
     2161 gatatttgtc aataagcata cgaaagtcaa agtattcgtt taaaattttc ggctcaacaa
     2221 taaccttatg cggctctttt tcaaagagga ttttgccttt gctgatgatc gcgtgcttga
     2281 gatacacatt atccgtgtcg tttatcaccg ccacatccac tttgttggtt tttaaagcct
     2341 gcgatagttt tcccatcaag ctaaacttaa tgtcgtatct cttttttatg tccggctcat
     2401 ccaaatagac cgcgaaatca taatcactca tcggcccgcg cgtgtttgtg gccgtggaac
     2461 cgaaaatata cgccaaaaca atttggggca tctcgcgcgc caaatctttc attaaatccc
     2521 gatatttttg caaatcattt tcaatatcat ccttcatatc ctcaacatac caaaaacccg
     2581 cccaaaataa aagaacgcga taattgcata ggtggcacct atgcaacttg attgcgattc
     2641 gcacccgcat aaaaaaagag ccccgagaat ttaaccctcg agggaacccg aagtgtcagc
     2701 ggttatggcg accgactgaa tgaagtgtat gacttcattg actacttttt gatgcttcca
     2761 aacttggtgg tggttaagca atgggaatct cgctactttt ccaaaaccgg tatttggaaa
     2821 cgatccgaag agtagctgtc cgccaatggc gaaccatcct ctgatttcaa gaattggcgg
     2881 ccaaaatttt ttctcttcaa gtgccccatc tatgaatttg agtccttcgc tgttattacg
     2941 gagtccggta attacagctt cattggtaag atcaaactta ccaaatttaa gaacccaatc
     3001 aggcgatcct ccgaacggca cgtcttcaag aataagaaag tctacgtcca ttgaataagc
     3061 agttatcatc gctataatac cgcccaacga atggccgctt aagccgatta aggcatttgg
     3121 attttcttca tatacctcat ctacttccag ctgtgcctct tcgacagctt caaagacggg
     3181 gatttttgtt aacccaaagg gccagacgcg taattctctg agttcgatat cccattcggc
     3241 atattttttt ctaagtgacg gaatatccac tcgtcttctt cgctgagctt tcatatcgtc
     3301 taccgtatat cccgcaatat gcaggattat tttctttctt cccaatcaaa tcacctcctt
     3361 tattattcaa ttttgattgg aactgggtgc accatagcat aaaattgggg ttttgtcaaa
     3421 tttgggacaa atgacaattt ggaaaaaatg gaaaaaattg acattgtctg tgcgggtttt
     3481 gtataataaa aatactaaag gaagttaatt gcgattatgg acaaatcgtc aaaaaaacaa
     3541 ccggtaactt tggagtcgct ggcgcaaacc atggcggcgg gattttcaac tctggacaag
     3601 aaaattgaca gcaagtttga ggttttggac aagaaaattg accgcaagtt tggtattttg
     3661 gacaagaaaa ttgacaccaa gtttgggatt ttggataaaa agattgacac caagtttggg
     3721 attttggata aaaagattga caccaagttt gggattttgg ataaaaaaat tgacgcggta
     3781 gataaaaaaa ttgatgatac ctccgaacag ttgttgataa aaatgcaaca gggattcttg
     3841 gaacagatcg agagaattga gtgtgttgag cgaagtgtta ccgaattgaa ggccgggcaa
     3901 gcgcggattg aaacaaattt agcgggtgcc gcacgcaata ttgatttgat ggatttgcgt
     3961 gccgaagtgg aaattttaaa acgaaaagtg taattttgaa acttaaaaac cgctccaaaa
     4021 ttggagcggt tttgataata attactttag ttcaaccccc gttatttcca cttcagtcaa
     4081 ctgctggcga tggccttttt tcagatgata gcgcttcttg gctttgtatt tataggcgat
     4141 caccttgggg cccttggttt gcgccacaat tttggcggac acggccacgc cggctaccac
     4201 gggctgtccc acggaaacct tcttatcatc cccttccacc aataaaactt cttcaaattt
     4261 aatcgcggca ccctcttctc ccgccaattt ttccaccttc aatttttgcc cgggagtcac
     4321 gatatactgc ttcccgccgg ttttgataac ggccaacatg aatttattat ttattaagat
     4381 tattctaact cgtttatact ataatgattt gctccaaaag tcaacaaata atgacataaa
     4441 taaattgtat ttgtattttg taggggcggg ctttatgtcc gcccgcatct caaggggcaa
     4501 ccacaagggt tgcccctaca aatgcgactt tttcgtattc tattaatcca aattcggttt
     4561 atcgatttgc ttcacttcca gtttatcccg gccgttatcg gtgattttta atacgtcttt
     4621 gtctattttg ccgaattcct tgtaggcggc gttataggtg ttcaccgagg ttgccagatg
     4681 gccgcccaga cgctgtaaac agtcattgta ggcgcacagg tgcttttcca gcatttccac
     4741 ccgttggcgg atctctttgg cggattcttc aatttgcaag gcgcgcaggc cttgcaggac
     4801 ggtttgcaaa taggccaaga agtttgtggg cgacacgatg atcacccgtt tctgagtgaa
     4861 ggcgtattca atcaagtcgc gggtgttgac tttcaccgcg ccaacctcgt taatcagcaa
     4921 atcataataa atgccttccg aggggataaa catcagcgcg aattccatcg tcccttcgcc
     4981 ggggcggatg tattttgaag tttcgtcaat gcgcattttc aaatcggcct tgaaaatctt
     5041 ttccagttcc cgcttgcggg tctcgtcttt ctcgttgatg attttgttgt agttttccag
     5101 cgaaaatttg gaatcaatgg gaatgatctt gtctttcacg aacaccgcgg cgtccacaat
     5161 ttcgccgttc ttgaacttgt attgcatctg aaacgattgc ggcggcaaaa catttttcag
     5221 cacggtttcc aaataatact cgcccaaaat cccccgctgt ttggggtttt tgagaatgtt
     5281 ttccagcgat tgcaattgtt gcgcaaaacc cactacctgc ttattggtgc tgtccaattc
     5341 ggtgagtttt tgggtaacat ccttgatcac ggcaaagctc tgcccgaact gctcctgcat
     5401 cgcccgattg gattcggcaa gcttggcgtc cagcgtttgc gacacctggc tcaactgctg
     5461 ctgcaacatc gccatcgccg cgtcattttt gggcagctcc tcccctttcc gcttccgcaa
     5521 caaaaaccac gtctgcattc ccagcgcgac aattacggca ataaaaagca ataaagttat
     5581 tgtatccata aatcttgaca agtattaatg gcttgctaca attggaatag aatgtccagg
     5641 aaaaagccca tcttcaggtt ccgtccttaa gcggagcttt ttcgtttata ttcaagcttt
     5701 tttttcaagt tttccataaa aactattttt ccttttgctg attttcttaa aagagaggaa
     5761 catttatctc tgtatgggct gataactttt tctaattttt gattttccaa taagcggtca
     5821 attaaacaat aaaaatctcc atcgctggtt acgatgagtc cattatcgta ttttggaaaa
     5881 tcaatcatcg cttgcaagac caaattggcg tcaatattgc ctttaatatc gccattatcg
     5941 tcagggatcg tgggtttaaa aactaaaaca taaccatatt tttgcaaaga cgaatatagc
     6001 ggttgaaaac tggcgatata tcccaaaaaa atataagcaa cttcaatgtg atatttttct
     6061 tttaaatatc tccgaaattt taaataatcc aatttccaac ccaaactttt cacccccaga
     6121 ttgaggttgt tgccgtcaat aaatgcgtaa ttttttaatt gcttatccat tatttataga
     6181 ttacgcccgc tcaatcaaaa aatcaaatta tatgatcaat ccttgtaatt ttaagaaaat
     6241 atgggataaa caagtaaact ggaggagtta tggctgattt aagcaatttt gggaatgggt
     6301 taatgggagc attcttattt ttaatgattg gaactggtgc tctattaact cttacggctg
     6361 aaaattggga ttggtatcaa ccgataattt tgcaaattgt tcatttattg ggtggtgatt
     6421 ggatttttgt gggagtgatg atggttatcg gttttattat cggttggatt atgcaagagt
     6481 tgtctccccc actttaaagc gataattatc ttatcgctct tttatttcaa aaacttcgcg
     6541 gtccaggtgt tggatttggc taattgttcc ggggggccgg tgaagatgat ttggccgccg
     6601 ttggcgccgc cgtccggacc catttccacg caccagtcgg cttctttgat gaaatcaaga
     6661 ttgtgttcaa tcacgatcac ggtgttgcct ttttccacca ggcggcgcaa aataatcagc
     6721 aattttttgg cgtcgtcaaa gtgcaggccc acggtgggtt cgtccaaaat ataaattgtt
     6781 cggccggtat cgcgcttggc cagttccgtg gccaatttca cccgctgggc ttccccgccc
     6841 gacaagctgg gcgcggattg ccccaactgc aaatagccca acccgatgtc ggaaagcgtc
     6901 tgcatcttgt cgttgatcgg cgggatatcg gcaaaaaagt ttttcgcttc gtcaacgctc
     6961 atttccaaaa catcggcgat gcttttgtcg cgatatttgg cgcttaacac ttcggcggtg
     7021 aaccgcttgc cttggcaagc ctcgcattcc acatagatat cgggcagaaa atacatttcc
     7081 accttttgcc agccttcgcc ggcgcaaacg ggacatcttc ctttttgggt gttgaacgaa
     7141 aaatggccgg gggtgaaccc ccggatttgg ctgtcgcgag tgcgcgtgta gagttcgcga
     7201 atataattga acacccccgt gtaggtcgcg gggttggagc gcggcgtttt gccgatgggg
     7261 ctttgatcca ccaaaaccgc tttgttgatg cgttcggttc cggccagcga ttcatatttt
     7321 cccggcaccg tccgggcgcg catcaaatcg cgcaacagcg ccgccgccag cgtttcaatc
     7381 accagcgtgc tttttcccga tccggaaacg ccacaaaccg cagtaagttt tccgagggga
     7441 attttcaagt ccgtgccctt cagcgtgaac tgtttggcac cggccaactc cagccacaaa
     7501 gtgcgttcac tcacctctct ctttttaaat cccgccgaaa tggacattct tcccgaaaga
     7561 tatttgccgg tgagggtttt ggcgcgcaag agctgtttgg gcgtgccttc aaaaaccagc
     7621 ttgccgccgt gcttgcccgc ggccgggccg atctcaatca cccaatcggc ggcttggatc
     7681 acctgctcgt cgtgctccac cacgatgaca gtgtttttca ggtcttttaa ttttttcagc
     7741 gcgccgagca ggcggtcaac atcccgctga tgcaatccca cggtgggctc gtccagcaca
     7801 taaaccacgc ccgagagggc cgatcccagc tggcaggcca agcggatgcg ctgattttcg
     7861 cccaccgaca gcgtggtggc ctcgcggccg agattgatat aatccacccc cacttcggcc
     7921 agaaaactca accgcttgca aatttcggcc aaaagcggcc gggcaatctc cttttgcttt
     7981 tcgggtaatt cggttttcag gttttccaaa aacacaatgg cgtcttttgc gggcaagcgg
     8041 caaacatcgg cgatgttgct gccagcgatt ttcacgctca acgcttcggg cgacaaccgc
     8101 gcgccgccgc aagccgagca ttctatttcg gacatatatt tggtaatttc cccgcgcaaa
     8161 tattccgaag tcgtttcgcg ataggcgcgt tccatgcggg agatgatgcc ttcatagctt
     8221 gggtcgctct tatcgccata gagcaccaat gtttgtatat gttcgggcag attgccaaac
     8281 ggctcgtcca acgaaaaact ttttgccgcc gccaaatttt gcaacgacca tttttgatgg
     8341 ggcacgccca ttgtgcgcaa tgaccatctt cccagcgaaa accacggcaa aatcgctccc
     8401 tgattcagcg acaggcgcgg gtttaaaatc agcgactcgt tgattttggc aattttcccc
     8461 agcccctggc aagttttgca cgcgccgtag gggctgttga aagaaaaaat tttgggctca
     8521 atgtcgggca aggaaatgtc gcaatcggga caagaaaagc gcgtggaaaa cgggcggtcg
     8581 gcgatgattt ttcctttgcc gatctccagc ccctttttta ccgccgccaa aattctttcc
     8641 ttctcttctt tcataaaatt ttcttgccgc tttttcttgg ccgccagcgc ttcccgttcg
     8701 cttttggaga tctgcttttt ttcgtttttg ggtttttcgt tgaattgccg caaatcaaaa
     8761 acgtccaccg ccacgccgat ggtgtgtgcc ttgtttctgt ccaaaacctt gtctttggct
     8821 tcggcggcgt cataggtgat gccgtcaatg atcacctttg gccagcccga gcggtagatt
     8881 tcttccaaga cgccggcatg cgcgcccttt ttggcggcta ccgccggcgc cacgaccgaa
     8941 acctcttttt tcaacgctgt ttgataaatt ttttcggcga tatgatcggg agtttgcgcc
     9001 gacactggcc ggccgcattt cggacaatga gcgacgccga tatgcgcgaa cagcaagcgc
     9061 agataatcat agatttccgt gagcgtgccc accgtggagc gcgggttgga ggaaatggtt
     9121 ttttggttga ccgcgatcgt gggcgacaac ccgctgattt tatccacatc cggctttttc
     9181 agcccgccca aaaactgccg cgcgtatgac gacaagctct ccaaataccg ccgttggcct
     9241 tcctgaaaaa tggtatcaaa cgccagcgac gacttccccg accccgatat accgcaaatc
     9301 accgccaatt tattcttggg gatcttgacg ctgatatttt tcagattgtg cacgcgaaca
     9361 cccgaaagct caatgaaatc c
//
LOCUS       DAOQWE010000020         9243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_7974_length_9243_cov_8.631911, whole genome shotgun sequence.
ACCESSION   DAOQWE010000020 DAOQWE010000000
VERSION     DAOQWE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7974_length_9243_cov_8.631911"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..282)
                     /locus_tag="PKK37_01355"
     CDS             complement(43..282)
                     /locus_tag="PKK37_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNR81061.1"
                     /translation="MDIEIITPKGVEFSGKAKRMILPAQSGQIAVLPDHMPLISVLKA
                     GKMKITAEGKEIEKEIEGGVLEVEKEKIVILLKKF"
     gene            complement(399..1760)
                     /gene="atpD"
                     /locus_tag="PKK37_01360"
     CDS             complement(399..1760)
                     /gene="atpD"
                     /locus_tag="PKK37_01360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNR81062.1"
                     /translation="MAKITQIIGPVVDAEFEEGKLPALGGALDVTGKNLILEVQQYLG
                     GNTARCLAMGPTEGLCRGQEVKDSGRTISVPTGKAALGRLFDVLGNPIDGMGDIKDGK
                     RYSVFREAPNYVQQKGEVEILETGVKALDLLCPMPKGGKIGLFGGAGVGKTVLIQELI
                     RNIAKVHKGVSVFAGVGERTREGNDIYLEMKESKTLDNTVLFFGQMSDQPGVRFRVPF
                     SAVAVSEYFRDEEKKDVLLFVDNVFRFVLAGCEVSALMGRIPSQTGYQPTLFTEMGAL
                     EERITSGQDGSITSMQAVYVPADDYTDPAVVAIFSHLDSSLVLSRNVASLGLFPAVDP
                     LATTSSILNPHIVGERHYRVASGVAQILQRYKELQDIIAILGVEELSDEDKLTVNRAR
                     RIQKFLTQPFFVAEVFSGKPGKYVKLEQTIEGFERIISGEFDDRPESDFYMKGGIEEV
                     IKN"
     gene            complement(1842..2714)
                     /gene="atpG"
                     /locus_tag="PKK37_01365"
     CDS             complement(1842..2714)
                     /gene="atpG"
                     /locus_tag="PKK37_01365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNR81063.1"
                     /translation="MELKEIRLKIESVANIRKLTGALETLSALKMKKSQKIALESRPF
                     AQRLGRLIRRLEPTLKDKVKLRKRKLRAALAVVIASDRGFCGSFNQNILKSAQKEIQK
                     LEKKSPVTVLPVGKKAIAFFKKRGREFGRSVTGIGDYGTLEELKPLSDFIIKSYIAND
                     YQRVYLIWTDFVSTFNQKPKMMQLLPLEWNELKEFACRSEDVAETAETVIEPSADMLV
                     KEIIPQLAQYLIYQCVLESNASEHSARMTAMRNASDNARERVAELTLEYNKARQEQIT
                     KEVCEISSAKEVLG"
     gene            complement(2809..4299)
                     /gene="atpA"
                     /locus_tag="PKK37_01370"
     CDS             complement(2809..4299)
                     /gene="atpA"
                     /locus_tag="PKK37_01370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNR81064.1"
                     /translation="MDKIEQFKRQLETSPLSAEINEIGQVVEVGDGIVKASGLMNVEN
                     FEMVEFERARVFGLVLNLEEYETGIIVLGPDDAIKEGDVVKRTQKTISVPVGPELVGR
                     VVDPLGRPVDGKGPIAAKETYPIERPAPGVIDRQPVDRPLHTGILAVDGLVPIGRGQR
                     ELILGDRGLGKTAIAVDMIINQKNEKNRPVCVYVACGQKKSKIKRLVKILEDAGAMEY
                     TIVVAAFADDPAALLYLAPYAGCAMGEWFRDHGRDAVAVYDDLTKQAWAWREVSLVLR
                     RPPGREAYPGDIFYLHSRLLERAVQLSEKLGGGSLTALPIVETQAGDISGYIPTNVIS
                     ITDGQIFLNSSLFYKGQRPAIDVGTSVSRVGSKAQTAAVKKVAGTLKLDLAQFQELER
                     FSEFTEELDAQTRRLLERGKRMMELLKQPDLRPLPFEVQAVIIFAGTKGLLDDLPLES
                     IATFKEKLVEELDSAPELAKKIRDTEKLENEVEAKLEEIIKKAQKI"
     gene            complement(4408..4800)
                     /locus_tag="PKK37_01375"
     CDS             complement(4408..4800)
                     /locus_tag="PKK37_01375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNR81065.1"
                     /translation="MNSKKAIKIWTAALIAECAGKTAAEQKKIILRLKEILKAKKRDY
                     LLAKIVTGALAEMKKRARFEIVFAREQSEETRKKLEKKLAARLDAVEDAEVKIDSSLI
                     GGFVAATDKYLVDASVKGQIEQLRKLKM"
     gene            complement(4809..>5273)
                     /gene="atpF"
                     /locus_tag="PKK37_01380"
     CDS             complement(4809..>5273)
                     /gene="atpF"
                     /locus_tag="PKK37_01380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNR81066.1"
                     /translation="DPKILIGNIATFVILAVILKKYAYRPFLAVLEKRRREIESGVAK
                     AQEAEKSLAKIRVLGEEIKSAGEKKAKETVAAAEIKAREKAAQILAAAEEEKKTALAN
                     ARAAMEKEAADARARRQEEALAVALAVSEKFLAQKITKDQDKKLIERLAQEL"
     assembly_gap    5274..5373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5452..5679)
                     /gene="atpE"
                     /locus_tag="PKK37_01385"
     CDS             complement(5452..5679)
                     /gene="atpE"
                     /locus_tag="PKK37_01385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNR81067.1"
                     /translation="MELEVMKQLAAALAIGLGAIGPGLGIGFIGRGAMEAIGRNPEAS
                     GKISTNMILAIAFTDAIAIFALVVALIIIFI"
     gene            complement(5722..5949)
                     /locus_tag="PKK37_01390"
     CDS             complement(5722..5949)
                     /locus_tag="PKK37_01390"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNR81068.1"
                     /translation="MNKLAAAMAIGLELGFLMALPLAVFLLLGVWLDKKFGTMPLFVI
                     VCLLAGLGAVFLEVKRIILPFLEKRSKNSDK"
     gene            complement(6083..6796)
                     /gene="atpB"
                     /locus_tag="PKK37_01395"
     CDS             complement(6083..6796)
                     /gene="atpB"
                     /locus_tag="PKK37_01395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNR81069.1"
                     /translation="MEEISVAAHTAFEIGGIAITNTGFWIILLAGGLAVLLPSVFAKA
                     KIVPGKIQNLLEFILETFLNFCDSITNSRKKTEEIFPFAMTLFLLIWVANLVELIPGL
                     GTFSILRAPSSDLNFTIALAALSIFYVNYLALKNLGLVPYLGKFFNFKNPILLFVGIL
                     ELMGEFTRIISLSMRLFGNLFAGEVLLIVVSTGIHWSLAYLVPLPFLGLETLVGFLQA
                     FIFSSLVVVFYSTATEAHA"
     gene            complement(6939..7538)
                     /locus_tag="PKK37_01400"
     CDS             complement(6939..7538)
                     /locus_tag="PKK37_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNR81070.1"
                     /translation="MPKKKFYAYRVGEEKGVTGNWGECAEIVGGQSGARYKSFESEDE
                     ARRWLEAGADYAVRHIAREPGIYFDAGTGGGNGVEISVTDSRGHGLLHRVLPEDELSA
                     CGHHLLERGRTNNFGELLACKYALQIALETGMEKIFGDSKLVLDFWSKGHIKKEMDGE
                     TIALAGEVAKLRKKFERLGGHMSRISGGANPADLGFHKN"
     assembly_gap    7539..7638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7640..8443)
                     /locus_tag="PKK37_01405"
     CDS             complement(7640..8443)
                     /locus_tag="PKK37_01405"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HNR81071.1"
                     /translation="MQKTSQILILAAALLILAAAAIFLLPLKSTPVVEAPSEPEIGNG
                     QNGEKIADEKYCNGFSPEACPEGCAVCPPCEVCSSIQCRLESSCAAIGFDKDWYHGNV
                     VIDETTPLPDNGGGQSENTGMANPASVYCVDQGGKLEIREGDGGQYGMCVFADGRECD
                     EWGFFRSGVCGDPSDGGTGAEVDNVVCGRENCHGLDIKCGPNPAEICTMEYQLGDKCL
                     RLVNCGIKDGVCQQIPNAAFDACKQCAQKCETDFANDQQGAFACEAKCE"
     gene            complement(8482..8748)
                     /locus_tag="PKK37_01410"
     CDS             complement(8482..8748)
                     /locus_tag="PKK37_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR81072.1"
                     /translation="MDDEVLICQSCGMPMAVSEDFGTDADGGQCRDYCRHCRQEGKFT
                     AEVELPEFIEMQVKIAVENLGMDEEEARAVAEMTLPELKRWQKE"
     gene            complement(8881..>9243)
                     /locus_tag="PKK37_01415"
     CDS             complement(8881..>9243)
                     /locus_tag="PKK37_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR81073.1"
                     /translation="TKENENFRKVIYTGKFSQLVVMSLKPGEEIGAEVHETHDQFFRF
                     EKGKAKVMIDDNEYQVEDDFAVIVPAGANHNVINVGDDEVKLYTIYSPAEHREGVVHA
                     TKEEAMADDEHFDGVTTE"
BASE COUNT         2107 a         2639 c         1939 g         2358 t
ORIGIN      
        1 aaaagccgcg cggccaattc gcgcggcttt ttaatgcgat gatcagaatt ttttgagcag
       61 gatgacgatt ttttcttttt ccacctccaa aacaccgcct tcaatttctt tttcaatttc
      121 tttgccttcg gcggtgattt tcatttttcc ggctttcaaa accgaaatca gcggcatatg
      181 gtcgggcaaa acggcaatct gccccgattg cgcgggcaag atcattcttt ttgcctttcc
      241 ggaaaattca acccctttgg gcgtgataat ttcaatatcc acggtaataa atttaaaatg
      301 taaaaatcaa aaattaaaat ttaggaattt ttatatatcg ttgaaatttt ttgaaaaaaa
      361 tttcaactcc aaaattttta atttttattt ttcaattttt aatttttgat gacttcctca
      421 atgccgcctt tcatatagaa atcgctttcc ggccggtcgt caaattcgcc cgagattatt
      481 ctttcaaacc cttcaatcgt ctgctccagc ttgacatatt tgccgggctt gcccgaaaaa
      541 acttcggcca cgaaaaacgg ctgggtcaaa aacttttgaa tccggcgcgc gcggttcacc
      601 gtgagtttgt cctcgtcaga caattcttcc acgcccagaa tcgcgatgat atcctgcaat
      661 tccttatagc gctgaagaat ctgcgccact cccgacgcga cgcgataatg ccgctcgccc
      721 acgatgtggg gattgaggat tgacgatgtc gtcgccagcg ggtccaccgc ggggaacaat
      781 cccagggaag cgacgttgcg gctcaacacc agcgacgaat caaggtggga aaagatcgcc
      841 accaccgccg gatcggtata gtcatcggcg ggcacataaa ccgcctgcat tgaagtgatg
      901 gacccgtcct gtccggaagt gatccgctct tccagcgcgc ccatttcggt gaacagcgtc
      961 ggctgataac cggtctgcga cggaatgcgc cccatcagcg ccgacacctc gcaacccgcc
     1021 agcacaaacc gaaacacatt gtccacgaac agcagaacat cttttttctc ttcgtcgcgg
     1081 aaatattccg acaccgccac cgccgaaaac ggcacgcgga aacgcacgcc cggctgatcc
     1141 gacatctggc cgaaaaacag cacggtgttg tccaaagtct tgctttcttt catctccaaa
     1201 taaatatcgt tgccctcgcg cgtccgctcg cccacgccgg caaacaccga cacgcctttg
     1261 tgaaccttgg caatgttgcg gatgagttcc tgaataagca cggttttacc cacgccggca
     1321 ccgccgaaca agccgatctt gccgcccttg ggcatcgggc acaaaaggtc aagcgccttg
     1381 acgccggttt ccaaaatttc cacctcgccc ttttgctgaa catagttggg cgcttcgcgg
     1441 aacaccgaat agcgcttgcc gtccttaatg tcgcccatac cgtcaatggg attgcccaac
     1501 acgtcaaaca gccggcccaa cgcggctttg ccggtgggca ccgagatcgt ccgtccggaa
     1561 tctttcactt cctggccgcg gcacagtcct tcggtgggac ccatcgccag acaacgcgcc
     1621 gtgttgccgc ccagatattg ctgaacttcc aaaatcaaat ttttgccggt gacatcaagc
     1681 gcgccgccca gcgccggcag tttgccttct tcaaattccg cgtccaccac cggaccgatt
     1741 atttgcgtga ttttagccat gtttcaaatt aaaaatttaa atatcaaaat taaaaatttt
     1801 ggaacttcct aaattttgat ttttgatttt tacattttgc attatcctaa cacttctttc
     1861 gccgaggaaa tttcgcagac ttccttggtg atctgttcct gccgcgcctt gttgtattcc
     1921 agcgtgagtt cggctacccg ctcccgcgcg ttgtccgacg cgttgcgcat cgccgtcatc
     1981 cgcgccgaat gttccgaagc gttgctttcc aaaacgcact ggtaaatcaa atattgcgcc
     2041 agctgaggaa taatctcttt taccagcata tcggcggaag gctcaatcac cgtttccgcg
     2101 gtttcggcaa cgtcttcgga ccggcaggca aattccttta attcgttcca ttccagcggc
     2161 agtaactgca tcattttcgg cttctggttg aaagtggaaa caaaatcggt ccaaatcaaa
     2221 taaacgcgct gatagtcatt ggcaatgtaa gatttgataa tgaagtccga caagggtttc
     2281 aattcttcca aagtgccgta atcgccgatg ccggtaacac tgcggccgaa ttcccggcca
     2341 cgctttttaa aaaaggcgat cgcttttttt ccgaccggca aaaccgtcac cggcgatttc
     2401 ttttccaatt tttggatttc tttttgggcg cttttcaaga tgttctgatt aaacgatccg
     2461 caaaacccgc ggtcgctggc aatcaccacc gccagcgcgg cgcgcaattt ccgcttgcgc
     2521 agtttcacct tgtctttcaa cgtcggctcc agccggcgga tcaatcgccc cagcctctgg
     2581 gcgaacggcc gcgactccag cgcgatcttc tgggattttt tcattttcag cgccgacaga
     2641 gtttccagcg cgccggtgag cttccgaatg ttggccaccg attcaatttt gaggcgaatc
     2701 tcttttaatt ccataaaaat gcaaaatgct ttgtattttg gggtctatcc ctcgttgcca
     2761 cgagggttag accccttcaa caaaaaccaa aacaactctt cgacaatttt aaattttttg
     2821 cgcttttttg atgatctctt ccaacttggc ttcaacttcg ttttccagtt tttcggtatc
     2881 gcgaatcttt tttgccaatt ccggcgcgga gtccagttct tccaccaatt tttctttgaa
     2941 cgttgcgatt gattccagcg gcaaatcgtc caataagccc ttggttccgg caaaaattat
     3001 cacggcttga acttcaaacg gcaagggtcg caaatccggt tgcttcaata attccatcat
     3061 ccgctttccc cgctccaaaa gccggcgcgt ttgcgcgtca agctcttcgg tgaattcgga
     3121 aaatctttcc agctcctgaa actgcgccag atcaagcttg agcgtgccgg ccactttttt
     3181 gaccgcggcg gtctgagcct tggaacccac gcgcgacacg gaagttccca catcaatcgc
     3241 cggccgctgg cccttgtaga aaagcgatga attcaaaaat atctggccgt cggtgatgga
     3301 aatcacgttg gtcggaatgt agccggaaat atctccggcc tgcgtttcca caatcggcaa
     3361 ggccgtaagc gatccgcctc ccagtttttc ggagagctgg accgcgcgtt ccagcaggcg
     3421 ggaatgaaga tagaaaatgt ctcccggata ggcctcgcgg ccgggcgggc ggcgcaaaac
     3481 cagcgacact tcgcgccatg cccacgcctg tttggtcaaa tcgtcataaa ccgccaccgc
     3541 gtcccggccg tgatcgcgaa accactctcc catcgcgcaa ccggcatagg gcgccagata
     3601 aagcagtgcc gccggatcat cggcgaatgc cgcaaccacg atcgtgtatt ccatcgcgcc
     3661 ggcatcttcc aaaattttca ccagacgctt gatttttgat tttttctggc cgcaagccac
     3721 ataaacgcaa accgggcggt tcttttcatt cttttggtta atgatcatat ccaccgcgat
     3781 cgcggttttg cccaatcccc ggtcgcccaa gatcaattcg cgctggccgc ggccgatcgg
     3841 caccaagccg tccaccgcca aaatccccgt gtgcagaggg cgatccaccg gctggcgatc
     3901 aatcacgccg ggagcggggc gttcaatcgg ataggtttcc ttggcggcaa tcggtccttt
     3961 gccgtccacg ggacggccca gcggatccac cacgcgcccg acaagttccg ggcccaccgg
     4021 cacggagatc gttttttggg tccgttttac cacatcgcct tccttgatgg cgtcatccgg
     4081 tcccaaaacg atgatcccgg tctcatactc ttccagattg agcaccaagc cgaaaacccg
     4141 ggcgcgctca aactccacca tttcaaagtt ttccacattc atcagcccgg aggccttgac
     4201 aatgccgtcg cccacctcca cgacctggcc aatctcgttg atttcggcgg acagcggaga
     4261 agtttccaac tggcgtttga attgttcaat tttatccatg tttcaaatta aaaatttaaa
     4321 tatcaaaatg aaaaattttg gagtcggaat ttttctccga aaaattccgg tgatatacaa
     4381 aaattcctga attttgattt ttgattttta catttttaac ttcctgagtt gttcaatttg
     4441 tcctttcacc gacgcatcca ccagatattt atcggtggcc gccacaaatc caccaatgag
     4501 cgacgaatca attttcactt cggcatcctc aacggcatcc aggcgcgccg ccagcttctt
     4561 ttccagtttt tttctggtct cctcggattg ctcgcgcgca aaaacaatct caaagcgcgc
     4621 tctttttttc atttccgcca gcgcgccggt gacaattttc gccagcagat aatccctctt
     4681 tttcgctttc aaaatttcct ttaggcgcaa aattattttt ttctgctcgg cggcggtttt
     4741 ccccgcgcat tcggcgatca gtgccgcggt ccaaatttta atcgcttttt tgctgttcat
     4801 aatccggatt ataattcctg cgccaatctc tcaatcaatt ttttgtcctg atctttggta
     4861 attttttgcg ccaaaaactt ttccgacacc gccaatgcca ccgccagcgc ctcttcctgc
     4921 cgccgcgcgc gagcgtcggc cgcctctttt tccatcgccg cgcgggcatt tgccagcgcc
     4981 gtcttttttt cctcctcggc cgccgccaga atttgggcgg ccttttcgcg ggccttgatt
     5041 tccgccgcgg ccaccgtttc cttggctttc ttctcgccgg cggactttat ttcttcgccc
     5101 aagacgcgaa tcttggccag cgatttttcc gcttcctgcg ccttggccac gcccgattca
     5161 atctcccggc gccgcttttc cagcaccgcc aaaaacggac ggtaagcgta ttttttcaag
     5221 atcaccgcca aaatgacgaa tgtggcaata ttgccgatta gaatttttgg atcnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatttttg cgcttcccga cttgtccctg
     5401 attcactccg aaacaagccg cggagaacaa aactccgccg tcttaaaaag actagatgaa
     5461 aatgatgatc aacgccacca ccagcgcgaa aatcgcaatg gcatccgtga acgcaatcgc
     5521 caaaatcata ttggtgctga tttttcccga cgcttccgga ttgcgcccga tcgcctccat
     5581 ggcgccgcgg ccgataaaac cgatacccaa tccgggcccg atggcgccca agccgatggc
     5641 caacgccgcc gccaattgtt tcattacttc aagttccata tttttggttg attaaattta
     5701 ttggttatca atgataattt atcatttatc actgtttttt gacctttttt caagaaacgg
     5761 caaaattatc cttttgactt ccaaaaaaac cgcgcccaac cccgccagca aacatactat
     5821 cacaaacagc ggcattgtgc caaatttttt gtccagccaa actcccaaaa gcagaaacac
     5881 cgccagcggc aacgccatca aaaatcccag ctccaatccg attgccatcg ccgccgccaa
     5941 cttattcata ataaattgaa aatgaaaaaa tcaaaatgaa aaatttttgt gttaaaattt
     6001 cgctaacgct tcattttaac atttaataat ttttaatttt gcattgtcat ttttaatttt
     6061 tatttttcaa ttttaaattt ttctacgcgt gcgcttccgt ggccgtggaa taaaacacca
     6121 caaccagcga ggaaaatatg aacgcctgca aaaaccccac cagcgtttcc agccccaaaa
     6181 acggaagcgg caccagatag gccagcgacc aatggatgcc ggtggacacc acaatcaaca
     6241 gcacttctcc ggcaaacaga ttgccgaaaa gccgcattga cagcgaaatg atgcgcgtga
     6301 attcccccat cagctccaaa attcccacga acagcaaaat cggatttttg aaattaaaaa
     6361 acttgcccag ataaggaaca agccccaagt ttttcagcgc cagataattg acataaaata
     6421 ttgataacgc cgccagcgca atggtaaaat tcaggtccga gctgggcgcg cgcaagatgg
     6481 aaaaagttcc cagaccggga atcagctcca ccagattcgc cacccaaatc agcagaaaca
     6541 gcgtcatcgc aaacggaaaa atttcttcgg tttttttgcg cgaattggtg atggaatcgc
     6601 aaaaattcaa gaaagtttcc aagatgaatt ccagcagatt ctgaattttt cccggcacga
     6661 ttttcgcttt ggcgaacacc gacggcaaaa gcaccgccaa gccgcctgcc agcaaaatta
     6721 tccaaaatcc ggtgttggtg atggcgatgc ctccgatctc aaacgcggtg tgcgccgcca
     6781 cggatatttc ttccatattc gtttctccct caaaaaaaca ccctctttct acgggttcac
     6841 ttaattttta ctcccttttt gctccaaagt caaatcttcc ccataattac ggacattctc
     6901 acattctcaa gaatattgga atgtccgcta acggaaaatc agtttttgtg gaagcccaaa
     6961 tcggcggggt tggcgccgcc cgagatgcgg ctcatatggc cgcccagccg ttcaaatttt
     7021 ttccgcaatt tggcgacttc gccggccagc gcgatggttt cgccgtccat ttcttttttg
     7081 atgtgcccct tggaccaaaa atccagcacc aatttgctgt cgccaaaaat tttttccatc
     7141 ccggtttcca gcgcgatttg cagggcgtat ttgcacgcca aaagttcgcc gaaattgttc
     7201 gtccgaccgc gctcaagcaa gtgatggccg caggcgctca actcatcctc gggcagaacc
     7261 cgatggagca aaccgtgccc ccggctgtcg gtaacgctaa tctccacgcc gttgccgccc
     7321 ccggtgcccg cgtcaaaata gattcccggc tcgcgcgcga tatgccggac ggcataatcc
     7381 gcgcccgcct ccagccaccg ccgcgcctca tcctcgcttt caaacgattt gtatctcgct
     7441 cccgactgcc cgcccacgat ctcggcgcac tcgccccaat taccggttac tcctttctct
     7501 tcgccgaccc gataagcata aaatttcttc tttggcatnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnat cattcgcatt tggcttcgca ggcaaacgcg ccttgctggt
     7681 cgttggcaaa atcggtttcg catttttgcg cgcattgctt gcaggcgtca aacgccgcgt
     7741 tgggaatttg ttggcaaacg ccgtctttta tcccgcagtt gaccaaccgc aagcatttgt
     7801 cgcccaattg atattccatg gtgcaaattt cggcaggatt gggcccgcat ttaatgtcca
     7861 acccatggca attttcccgg ccgcagacaa cattatcaac ctccgcgccg gtaccgccat
     7921 ccgatggatc gccgcaaaca ccactgcgaa aaaatcccca ttcatcgcat tcccggccgt
     7981 cggcaaaaac gcacattccg tattgcccgc catcgccttc ccggatttcc agtttgccgc
     8041 cctggtcaac gcaataaacc gaggcgggat tggccatgcc ggtattctcg ctctgtccgc
     8101 cgccgttatc cggcaatggc gttgtttcgt caatcaccac attgccgtga taccaatctt
     8161 tgtcaaaccc gatcgccgcg cacgacgatt ccaagcggca ttggattgaa ctgcaaactt
     8221 cgcacggcgg gcaaaccgcg catccctccg gacaggcttc gggcgaaaag ccgttgcaat
     8281 atttttcatc cgcgattttc tcgccgttct gtccgtttcc aatttccggc tccgacggcg
     8341 cttccaccac cggcgtgctt tttaaaggca acaaaaatat ggccgcggcg gccagaatca
     8401 aaagcgcagc ggccagaatc aaaatttggc ttgttttttg catatattat tggcaattta
     8461 aaactttcgc gcgaaaacga ttcactcttt ttgccagcgc ttcaattccg gcaaagtcat
     8521 ttcggcgacc gcgcgggctt cctcctcatc catacccaaa ttttccaccg caattttcac
     8581 ttgcatctca ataaactccg gcaactccac ctcggcggta aattttccct cttggcggca
     8641 atggcggcaa taatcccggc attgcccgcc atcggcgtcg gtgccgaaat cttcggaaac
     8701 cgccatcggc atgccgcaac tctggcagat taaaacttcg tcgtccataa ttttgttttt
     8761 atgattttat acttctatat atgattatat ggctttatgc ttatcagcat atcaaaaaca
     8821 gcaccgtttg caacgcaaaa aacgcggatt cccgcgtttt ttgtttgccg accggattta
     8881 ttactcggtg gtgacgccgt caaaatgctc gtcgtcggcc atcgcctctt ctttggtggc
     8941 gtgcaccact ccctcgcggt gttcggcggg agagtagatg gtgtagagtt tcacctcatc
     9001 gtcgccgaca ttgatcacat tgtgattcgc gcccgcgggc acgatcaccg caaaatcgtc
     9061 ttccacttga tattcgttat cgtcaatcat caccttggct tttccctttt caaagcgaaa
     9121 aaactgatcg tgcgtttcgt gaacttcggc gccgatttct tcgccgggtt ttaaactcat
     9181 caccaccagc tggctgaact ttccggtgta aatcaccttg cgaaaatttt cattttcctt
     9241 ggt
//
LOCUS       DAOQWE010000021         8868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_8369_length_8868_cov_7.697152, whole genome shotgun sequence.
ACCESSION   DAOQWE010000021 DAOQWE010000000
VERSION     DAOQWE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8369_length_8868_cov_7.697152"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PKK37_01420"
     CDS             complement(<1..890)
                     /locus_tag="PKK37_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81074.1"
                     /translation="MIFWLVMLLFLPIALASSPTVSCVGCDDRKTGEITTDGTNSWQT
                     FYAKEGSRITLQGSSGFWEDDSGRKISDGSSLEFSVKDSTERVLMSPSGDFHATISLV
                     LLSSCVPEWRSGIKINGDEYGKDDTRETAAGEELSFSVRIGSGCKNYRVEWSANPPLA
                     VAFDNSRSLSTKGYVAKDYSGKNPVVTVAIISENGERRREQTVNLMVEKNSAPAIKVR
                     VGSSIASYTSFEVSFEGSTTGDSGNEDGDYIAEIEAWLKDANGKSVSHSRRTMDREKK
                     LPTLSLTPGSVGQYTLTATIT"
     gene            complement(996..1898)
                     /locus_tag="PKK37_01425"
     CDS             complement(996..1898)
                     /locus_tag="PKK37_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR81075.1"
                     /translation="MKKGLFLAVFFVLAAVLTVLPLGKNFAAAQNYGALNPAAFIESL
                     KTELSLLKMLLTNMNAKSEINSPAYIAVDLESGKTLLSKNSEQARPAASITKMMTATI
                     ARENIDENKKITLTDSMLAPAGNSSVLYAGREIDIKTLLKAALIQSSNDAAEALAQAA
                     GKENFLALMNQKAAELGMENTVFFDPCGLNPKNKSTPADLAKLVAYVHKNHPEILAIG
                     KSNDFWLEDKSGVWMKFQNVNNFYPLAAFVGGKTGYLPEAKQTIAGVFNVNGKPTAIV
                     VLYSANRQADVFRILEKLQSQGGL"
     gene            complement(1928..2479)
                     /locus_tag="PKK37_01430"
     CDS             complement(1928..2479)
                     /locus_tag="PKK37_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNR81076.1"
                     /translation="MKYQSIVFSGLPGAGKSTLVAKLKEIYKWPVLSVGDLWRARWAN
                     LYPDKKISFEEYWRTTSSEDNRQVNVDFREQAAINHLLGDSRYTTYLKDLPVLLIFLT
                     ADLDTRAKRGVGLAKYNGSCEEEIKNILYRREADEVAAGKRLFGYDYRDSANYHLVLN
                     TALVSQEEEIAIIKTLVSPENNR"
     gene            complement(2481..3539)
                     /locus_tag="PKK37_01435"
     CDS             complement(2481..3539)
                     /locus_tag="PKK37_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNR81077.1"
                     /translation="MSPKIFNPQKLEVFMEKGSQRGFVTFAEIMSFFPEVEKDIDGLD
                     ELYQILEAKGIEVKEAREFLEVGEKVAKRRLESKIDPVQMYLKEIGRIPALTAHDEKE
                     LAKKIEAGDAEARKRLTQSNLKLVVSIARKYIGRSAHLGLLDLIQEGNLGLFRAVEKF
                     DWRKGYKFSTYATWWIRQAITRALADQARTVRIPVHMIETISKYTQAKRKLLQFMGRE
                     PLPEEIASELGLEPAKVRYIQKISQDSVSLETPVGDDGEDSVLAEFIADHKVTSPALD
                     AARSLLKNRLNEVLIDLMPREQKILALRFGLEDGVTHTLEEVGQEFGVTRERIRQIEA
                     KALEKIRNHASLKKLRDY"
     gene            complement(3732..5531)
                     /gene="dnaG"
                     /locus_tag="PKK37_01440"
     CDS             complement(3732..5531)
                     /gene="dnaG"
                     /locus_tag="PKK37_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNR81078.1"
                     /translation="MDSPIEEIKSRLDIVEFVGQYLKLKKTGANLSALCPFHSEKSGS
                     FFVSPARQTWKCFGCGKGGDIFTFVQEIEGVEFGDALRILAAKAGVELKRQTRESAQM
                     KTERQRLYEACELAACFFEKQLASSKTGAAAKKYLLDRGFSEKSIAAWRLGWAPSSWQ
                     GLHDFLASQGYTDKEIVGAGLAAASAGGRVYDRFRGRIMFPVFDFNSQTVGFGGRVFE
                     NVADDVRETGAPPAAAEPKYLNTARTALYDKSRLLYGLDRAKVAIRQADACVLVEGYA
                     DAIMSAQAGVENVVATSGTALTEDHLKIIKRFTNNLILGYDMDLAGENASRRGIDMAR
                     AAGFDVRVARPAIAGKDPADIAAADPREWQKATAGAKSIMEFYFDRALAAGDKNSPAG
                     RKKIVDFILPAIKIIPNAIEQAFWLQKIADKMEARDIHYEEYLRQELKKVRINQNGPA
                     AAAVSRVASAANAKSRETRLEERILTLAVKFPVHIGLVGDAPDFFTDEGKEIAARLRA
                     GGFPKGEIAGKVGEYYETLFIAAENETVSEAAAAEEIEFHVAAIRENLFKNSLARCAR
                     DLIRAETAGDLETAGNLREKYNKLCKKTKNSVN"
     gene            complement(5542..6255)
                     /locus_tag="PKK37_01445"
     CDS             complement(5542..6255)
                     /locus_tag="PKK37_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81079.1"
                     /translation="MDNNQFLKEIKKSFIVFLKTHSRSNKKLIVLHGSIASDIKNKLG
                     HEYGVKSLGVENNKEGRMQGRYMEKTVDILISKNNKDIAGIGIKFVMNNYSQNSNNYF
                     ENMLGETANIRSNNKDYFQILILPEDLPYYNNKGKIIKWEKITSHNISKYIVLSEDDA
                     AKYFHTPVKTLLFIIRFPKCDHNSIASKTAYKKYYLSRSSNINMRISDKIAGEFGRAM
                     ILNDYETFIDKIIHYIKSI"
     gene            complement(6273..7736)
                     /locus_tag="PKK37_01450"
     CDS             complement(6273..7736)
                     /locus_tag="PKK37_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNR81080.1"
                     /translation="MDISVQKQNGVVFTPQWVADFMVSEILKNQKIIGNEKILDAGCG
                     EGVFSAAAVRKFSELSGKPVERVIEENIYFIDISPDYIKTTKKNLQKLSKEKILKFND
                     ILADFCFYNFSQKFDYIIGNPPYVRIQNLNERRAQLQEKFKTASNGSIDLYYCFFEQA
                     LKIIKNYGKICFITPNSHFHSFAGKNLRDLLSPYLEKIVDFDYFQVFGDATTYTAITF
                     LNKDKPSMFLYAQNFKNDFSNLEYRQISQKNIRSERWEFFSDDYSQKIKELNKKYSKL
                     NEVADIHYGIATLKDDVYIFSPEKFDDSYFYFKGYKIEKSLCVPIIKASTYKGLDQNL
                     YLIFPYENEKIISQKKFQEKYPNAYNYLSDHREILESRDKGGGKNYEEFYAFGRSQGL
                     KTSFGKKIITSTMNIAPRFYVIENKKTSFFAGYCVKPKYEYIDIYQLCDALNSDFMKE
                     YIESVSRSYRGGYKSYAKSFLKDFVHPYFFKSQPTLF"
     gene            7954..8463
                     /locus_tag="PKK37_01455"
     CDS             7954..8463
                     /locus_tag="PKK37_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNR81081.1"
                     /translation="MKIAIISDTHDNLATLDKFLAHIKKNPVGAIIHCGDIAEGETLA
                     RLAKNFPGPILAVFGNMDYRQSVEAAAQKFSGQIQLFKDFGHTHIGGLRIGFCHFPEA
                     AKRACENNRFDFVFYGHTHKPWLETINGCQLANPGTLAGMFYQATFAILDAGTKKLEL
                     KIVSRLALT"
     gene            complement(8530..8808)
                     /locus_tag="PKK37_01460"
     CDS             complement(8530..8808)
                     /locus_tag="PKK37_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HNR81082.1"
                     /translation="METTAFPQYISRFLPVCEPLPEIGMWFFAILFAWSIAWKGAALW
                     RAARNNQSYWFTAIILINTVGILEILYIFIFAKKRPESGASGQSVQIK"
BASE COUNT         2244 a         2201 c         1860 g         2563 t
ORIGIN      
        1 gtgatggtag cggtcagagt atattgcccc acacttccgg gcgttaaact caaggtcggc
       61 aatttttttt cccgatccat ggtcctccgg ctgtggctca ccgatttacc attagcgtcc
      121 ttcagccagg cttcaatttc ggcgatataa tcgccgtctt cgttgccaga gtcgccggtg
      181 gttgaacctt caaacgaaac ctcaaaagat gtgtatgagg caattgaaga accgactctg
      241 actttaatgg ccggcgcgga atttttttca accatcagat tgacggtctg ttcgcgccgc
      301 ctttctccat tttcagagat gatggctacc gttaccacgg gattcttgcc cgaataatcc
      361 ttggccacat atcctttggt ggaaagcgac cgggaattat caaaggcaac cgccaaaggt
      421 ggattcgcgc tccactcaac ccgataattc ttgcaaccgc tcccaatcct cacactaaaa
      481 ctaagttctt ctccggccgc ggtctcccgg gtatcgtctt tgccgtattc atcgccgttg
      541 atcttaatcc ccgatcgcca ttcgggaacg catgatgata gcaaaacgag acttatcgtc
      601 gcgtgaaaat ccccgcttgg cgacatcagg acacgctcgg tgctatcttt gacagaaaat
      661 tccagcgaac ttccgtcact gatttttcga ccgctatcat cttcccaaaa gccggaactg
      721 ccttgcaatg taatccggct accctccttg gcataaaaag tttgccaaga gttagttccg
      781 tcagtggtga tttcacctgt cttcctatca tcacaaccaa cgcaagacac cgttggcgaa
      841 ctcgccaacg caatcggcaa aaacagcaac attaccaacc aaaaaatcac aatcgttttt
      901 gacaacaatt cctttgcctc cttttttagt ttttgacaat gaactttatc ttgcggctat
      961 tctgaactta attttaaatt tgtcaagatt gaggatcata atcccccttg agattggagt
     1021 ttttccagga tgcggaaaac atcggcttgg cggttagcgg agtagagaac aacaatcgcg
     1081 gttggtttgc cattgacatt gaacacgccg gcgatagtct gcttggcctc gggcaaatag
     1141 ccggttttgc cgccgacaaa cgccgccagc ggatagaaat tgttcacatt ttggaatttc
     1201 atccacacgc cggatttgtc ttccagccaa aaatcattgc tcttgccgat ggccagaatt
     1261 tcgggatgat ttttgtggac ataggccacc aatttcgcca aatcggcggg cgttgatttg
     1321 tttttgggat tcagtccgca aggatcaaaa aacacggtgt tttccatccc caattcggcg
     1381 gctttttggt tcatcagtgc caaaaaattt tctttgcccg cggcctgcgc cagcgcttcg
     1441 gcggcatcgt tgcttgactg aatgagcgcc gccttgagaa gagtcttaat gtcaatttcc
     1501 cgcccggcgt aaaggacgga gctgtttccc gccggcgcca gcattgaatc ggtgagagtg
     1561 atttttttgt tttcgtcaat attttcgcgc gcaattgttg cggtcatcat tttggtgatt
     1621 gacgccgccg gccgggcttg ttccgaattt tttgacaaca gtgttttgcc gctctccaaa
     1681 tccaccgcga tatacgccgg agaattgatt tcgcttttcg cgttcatatt cgtcaaaagc
     1741 atttttaaaa gcgaaagctc cgttttgaga gattcaataa atgccgccgg attgagcgcg
     1801 ccgtaatttt gcgccgcggc aaaatttttc cccaatggca acaccgttaa aactgcggca
     1861 agaacaaaaa atacagctaa aaataatcct tttttcatga aaattttata ttttaccgaa
     1921 acagacctca acggttattc tcaggggaaa ccaaagtttt gatgattgca atttcctctt
     1981 cttgcgaaac caaggcagtg tttaaaacca gatggtagtt tgccgaatca cgataatcat
     2041 atccaaaaag acgcttgccg gcggccacct catcagcttc ccggcgataa agtatgtttt
     2101 tgatttcctc ttcgcaactg ccgttatatt ttgccagccc cacgccccgt ttggcccggg
     2161 tgtctaaatc cgcggtcaga aatatcaaca ataccggcaa atctttcaga taggtcgtat
     2221 aacggctgtc acccagcaaa tgatttattg ccgcctgttc gcgaaaatca acattcacct
     2281 gccgattgtc ttcactagaa gtggtgcgcc agtattcttc aaaggaaatt tttttgtcgg
     2341 gatataaatt tgcccagcgc gcccgccata aatcacccac cgacagcacc ggccatttat
     2401 atatttcttt taatttcgcc accagcgtgg attttcccgc cccgggcagt ccggaaaaga
     2461 cgatggattg atatttcata tcaatagtcg cggagttttt tcaatgaggc atggttgcgg
     2521 attttttcca gggctttggc ctcaatttgg cggatgcgct cgcgcgtcac gccgaattcc
     2581 tgccccactt cttccaaggt gtgcgtcacg ccgtcttcca agccgaagcg caacgccaga
     2641 attttttgct cgcggggcat caaatcaatc aacacttcgt tgagccggtt tttgagcaaa
     2701 ctgcgcgcgg cgtccagcgc cggcgatgtc actttgtgat cggcgatgaa ttccgccaac
     2761 acgctgtctt cgccgtcatc gcccacggga gtttccagcg acaccgaatc ctgggaaatt
     2821 ttttggatat agcgcacctt ggccggctcc aatcccaatt ccgaggcaat ctcttcgggc
     2881 aaaggttcgc ggcccataaa ctgcaacagc tttctttttg cctgcgtgta tttggaaatg
     2941 gtttcaatca tatgcaccgg aatgcgcact gtccgcgcct ggtcggccag cgcgcgggtg
     3001 atcgcctgcc gaatccacca agtggcgtag gtggaaaatt tgtaaccctt gcgccagtca
     3061 aatttttcca ccgcgcggaa caaacccaaa ttgccttcct ggatcaaatc cagcaagccc
     3121 aaatgggcgc tgcgcccgat gtatttgcgg gcgatggaca ccaccaattt caaatttgat
     3181 tgagtgagcc gcttgcgggc ctcggcgtcg ccggcttcaa tttttttcgc cagctccttt
     3241 tcgtcatggg cagtgagcgc cggaatccgg ccgatttctt tcaagtacat ctgcaccgga
     3301 tcaatttttg attccaggcg gcgcttggcg actttttcgc cgacttccaa aaattcccgc
     3361 gcctctttga cttcaatgcc tttggcttcc aaaatttggt acagttcgtc cagcccgtca
     3421 atatcctttt ccacctccgg gaaaaaagac attatctcgg caaacgtcac aaacccccgc
     3481 tggctccctt tttccataaa aacctccagc ttctgcgggt tgaaaatttt gggcgacatt
     3541 ccctccttct tgcgcgctat tttttttgcc ttcggcgcgg cggctttgga aggttttttg
     3601 gccacccggg gtttttgctt accggcgggt ttgcccgcgg gcactttctt tgcctgcggt
     3661 tttttcgcgc ggacggccga cgctttcggc gcggacttca ccggtttttt tgttttgctc
     3721 gccatatcgt tttaattaac cgagttcttt gtttttttgc aaagtttgtt atatttctcg
     3781 cgcaaatttc cggcggtttc aaggtcgccg gcggtttccg cccgaatcaa atcgcgggca
     3841 cagcgcgcca aagagttttt gaaaagattt tcccggatcg cggcaacgtg aaattcaatc
     3901 tcttcggccg cggcggcttc gcttacagtc tcgttttccg cggcgataaa aagcgtttcg
     3961 tagtattctc caaccttgcc cgcgatctcg cccttgggaa acccgccggc acgcaagcgc
     4021 gcggcaattt ctttgccctc gtcggtgaaa aaatccggcg cgtccccgac caaaccgata
     4081 tgaacgggaa atttaacggc cagcgtcaaa atccgctctt ccaagcgggt ttcccggctt
     4141 ttggcgttcg cggcggaagc aacgcgcgaa acggccgcgg cggcgggtcc gttttggtta
     4201 atcctaacct ttttcagctc ttggcgcaag tattcttcat agtggatgtc gcgcgcttcc
     4261 attttgtcgg cgattttttg cagccaaaac gcctgctcaa tcgcgttggg aatgatcttt
     4321 attgccggca aaatgaaatc aacgattttt ttccgccccg ccggagaatt tttgtcgccg
     4381 gcggcaagag cgcgatcaaa ataaaattcc ataatgctct tggccccggc agtcgctttt
     4441 tgccattccc gcggatcagc cgccgcgata tcggccgggt ctttgccggc aatcgccggt
     4501 cgcgccacgc gcacgtcaaa tcccgcggcg cgggccatat caatgccccg ccggctggca
     4561 ttctcgccgg caaggtccat atcatagccc aaaatcaaat tgttcgtgaa tcgtttgatg
     4621 atttttagat ggtcttcggt gagcgccgtg ccggaagtcg cgacgacatt ttccacgcca
     4681 gcttgcgccg acataatcgc atcggcatat ccttccacca aaacgcaggc atcagcttgg
     4741 cggatcgcca ccttggcgcg atccagaccg taaagcaaac ggcttttatc gtaaagcgca
     4801 gtgcgggcgg tgttcagata tttcggttcc gccgcggcgg gcggcgcgcc cgtttctcgg
     4861 acgtcatccg ccacgttctc aaaaacgcga ccgccaaacc ccaccgtttg cgaattgaaa
     4921 tcaaagaccg gaaacattat ccggccgcgg aaccggtcat aaacccggcc gcccgccgac
     4981 gcggcggcca atcccgcccc gacaatctcc ttgtcggtgt agccttggct tgccagaaaa
     5041 tcgtgcaatc cctgccagga agacggcgcc catcccaaac gccacgcggc gatgcttttt
     5101 tcggaaaatc cccggtcaag caaatatttt ttcgccgcgg cgccggtttt tgacgaagcc
     5161 agctgttttt caaaaaaaca cgccgccagt tcgcaggctt cgtaaagccg ctggcgttcg
     5221 gttttcatct gcgcgctttc gcgggtctgg cgcttcaatt ccacgccggc tttcgccgcc
     5281 aagatccgca aggcatctcc gaattcaacc ccctctattt cctgcacaaa agtgaaaatg
     5341 tccccgccct tgccgcaacc gaagcatttc caagtctgcc gcgccggcga aacgaaaaac
     5401 gacccgctct tttccgaatg gaacgggcac agcgccgaaa gattggcgcc ggtttttttg
     5461 agcttcaaat attggccgac aaattccaca atgtccagcc ggctcttgat ttcttcaatc
     5521 ggcgaatcca tgtttttgcc attaaatgct tttaatataa tgtattattt tgtcaataaa
     5581 cgtctcgtaa tcatttaaaa tcatcgcgcg cccaaactcg cccgctattt tatcggatat
     5641 tcgcatattg atattgctac ttcggctaag ataatatttt ttgtaagcag tttttgaagc
     5701 tatggaatta tggtcgcatt ttggaaatct aatgataaat aataatgttt taaccggcgt
     5761 atgaaaatac tttgccgcat catcttccga taaaacaatg tacttgctga tattatggct
     5821 ggtaattttt tcccatttta ttattttgcc tttattgttg tagtaaggta agtcttcggg
     5881 taatattaag atttgaaaat aatctttgtt attcgatcta atgttcgctg tctctcccag
     5941 catattttca aaataattat tggaattttg agaataatta ttcattacaa attttattcc
     6001 aattccagca atatctttgt tattttttga aatgagaata tcaacagttt tttccatgta
     6061 tctaccctgc attctgcctt ctttattatt ctcaacgcca agagatttaa ccccatattc
     6121 atgccctaat ttatttttaa tgtcactggc aatggaacca tgcaagacaa tcagtttttt
     6181 gttgctccgt gaatgtgttt tcaaaaatac aataaaggat tttttaattt ctttcaaaaa
     6241 ttgattgtta tccataatat ttgagtttta agttaaaata aagttggctg tgatttgaaa
     6301 aaataaggat ggacaaaatc ctttaagaaa cttttggcat aagacttgta tccgccacga
     6361 taacttcttg aaaccgattc gatatattct ttcataaaat cggaatttaa cgcatcgcaa
     6421 agctggtaaa tatcaatgta ttcatactta ggcttcacgc aataaccggc aaaaaaactc
     6481 gtttttttat tttcaataac ataaaatcgc ggcgcaatat tcatcgtact ggtaatgatt
     6541 tttttgccaa aacttgtttt taatccctgg ctacgcccaa aagcataaaa ttcttcgtaa
     6601 tttttgcctc cgcctttgtc tcttgactct aaaatctcgc gatgatcgga aagataatta
     6661 taggcattgg gatatttttc ttggaatttt ttttgcgaaa ttattttttc gttttcataa
     6721 ggaaaaatca aatataaatt ttgatccaat cctttataag ttgaggcctt aataatcgga
     6781 acacataatg atttttctat tttataacct ttaaaataaa aatagctatc atcaaatttt
     6841 tctggagaaa aaatataaac atcgtctttc agcgttgcaa ttccataatg aatatcggca
     6901 acttcattca gttttgaata ttttttgttt aattctttaa ttttttgcga gtaatcatcg
     6961 ctgaaaaatt cccatctttc cgagcgaata tttttttggg atatttgcct atattcaaga
     7021 tttgaaaaat catttttaaa attttgtgca tataaaaaca tgcttggctt atctttattc
     7081 aaaaaagtta ttgccgtata tgttgttgca tcgccaaaaa cttgaaaata gtcaaaatca
     7141 acaatttttt ccaaatatgg tgacaataaa tcccgcaaat ttttaccggc aaaagaatga
     7201 aaatgagaat tgggcgtaat aaagcaaatt ttgccgtaat ttttaataat tttcaaagct
     7261 tgctcaaaaa aacaatagta aagatctatt gaaccgtttg aagctgtttt aaatttttct
     7321 tgcaattgcg cacgtctttc attcaaattc tgtattctca catagggagg attgccgata
     7381 atataatcaa atttttgtga aaaattataa aagcaaaaat ccgctaaaat gtcattgaat
     7441 tttaaaattt tttcttttga taatttctgc aaattttttt ttgttgtttt aatataatct
     7501 ggagagatat caataaaata tatattttct tcaatcactc gttcaaccgg cttgccggac
     7561 aattcagaga attttctgac tgcggccgcc gaaaacacgc cttctccaca accggcatcc
     7621 aaaatttttt cattaccgat tattttttga ttttttaaaa tttcactcac cataaaatcc
     7681 gccacccatt ggggggtaaa aaccacgccg ttttgttttt ggaccgaaat atccataatt
     7741 ttatcgcaaa ccaagcccct ctcaatggct aaaatttatc tgctgatccc ttttgcaatt
     7801 ttgtttgatt ggccaacacc gtcattgtag ccgaaaatcg ggcaaaaatc caccgaccgg
     7861 gaaatatgtt ataatggttg cgtcaaaatg caatttgcca aggtcgcacc ttgccacgct
     7921 cgtcaaggaa cgacctttta aaatgcaatt ccaatgaaga tcgcgatcat ttcggacact
     7981 catgacaact tggcgacgct ggataagttt ctggcgcaca tcaaaaaaaa tccggtcggg
     8041 gcaataatcc attgcggcga tattgccgaa ggcgaaacct tggcgcggct ggcgaaaaat
     8101 tttccggggc cgattttggc ggtttttggc aatatggatt atcgccaatc ggttgaagcg
     8161 gcggcgcaaa aattttccgg ccaaatccaa ttattcaaag atttcggaca tacccatatc
     8221 ggcgggttga gaatcggctt ttgccatttt ccggaagccg ccaagcgcgc ttgcgaaaat
     8281 aaccgctttg attttgtgtt ttacggccac actcacaaac cgtggctgga aacaatcaac
     8341 ggctgccagc tggccaatcc cggaaccctg gcgggaatgt tctatcaggc gactttcgcc
     8401 atattggacg ccggcacgaa aaaattggag ttaaaaatcg tcagccggct agcgctcact
     8461 taaagtgatc gctaaaaatt cggcaaagta aaacaaaaaa cgccccgtga agggcgtttt
     8521 gccgcaaacc tatttgattt gcacactctg gccggaggcg ccggattcgg gacgcttttt
     8581 ggcaaaaatg aaaatgtaaa gaatttccag aatgcccacc gtgttgatca gaatgattgc
     8641 ggtaaaccaa tatgattgat tgttgcgagc ggcgcgccac aatgccgcac ccttccacgc
     8701 gattgaccac gcgaacaaaa tcgcgaaaaa ccacataccg atttccggca aaggttcgca
     8761 aacgggcaaa aaccgactga tatattgagg aaaagccgta gtttccataa attttattat
     8821 tagacctact gcttaaatat ccactactgc aatttccata tttcggct
//
LOCUS       DAOQWE010000022         8733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_8528_length_8733_cov_7.691864, whole genome shotgun sequence.
ACCESSION   DAOQWE010000022 DAOQWE010000000
VERSION     DAOQWE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8528_length_8733_cov_7.691864"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..2233
                     /locus_tag="PKK37_01465"
     CDS             41..2233
                     /locus_tag="PKK37_01465"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNR81083.1"
                     /translation="MENSSSRYNPKETEQRIYNDWEKKGYFNPDKSNPGTKKVFSVVL
                     PPPNVTGTLHMGHALNITIQDALVRYHRMKGETTVWMPGSDHAGIGLQSTLEKKLRKE
                     GVSRFDLGRDEFIKKAWEWKELYGNIILNQIRAMGASCDWSRTKFTMDEDYVNAVETV
                     FIHYHRQGWVYRGQRVVNWCPRCGTSISDLETDYVAEASKLWHIKYPIASFSPKTKAD
                     EIGEYIIVATTRPETMFGDTAVAVNPKDERYRALIGKKAVLPVVGREIPIIADKSIDI
                     DFGTGAVKITPAHDLNDWQIGQKHKLPLIAVIDERGKMNGNTPAEYQGLKTGEARNKL
                     AAHLQEIGLLEKIEDYEHQVPKCSRCGAAIQLIPSMQWFVKMDDLARKALIAANSGKI
                     KFHPKRWESTYHSWLKEPRDWCVSRQLWWGQRLPVWFCQTEPDKYTVAKSEPSKCPIC
                     GKCRPKRSEDVFDTWFSSALWPLAVFGWPKITQDLKKFYPTSVLTTGRDIINLWVARM
                     AFSGMEFMNKAPFAQVIINAMVLTKDGKRMSKSLGTGIDPMTLIEKYGTDATRFGLVW
                     QVSESQDLHFNEDNMLAGQKFCNKIWNASRFVCGQIEGEKNIYSLTVKPKPITSADKK
                     IMAGLAKICRSTEKNINRYALGAAIRDLYDFFWHKFCDVYIEAAKNQIRGAKNKTQTR
                     NTQKILIYVLANSLKLLHPFMPFVTEELWQTLPLKTKTDLIISPWPKS"
     gene            2314..2973
                     /gene="radC"
                     /locus_tag="PKK37_01470"
     CDS             2314..2973
                     /gene="radC"
                     /locus_tag="PKK37_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNR81084.1"
                     /translation="MKIRDLAKVDRPREKLEKYGPEKLATAELMAVLLGSGIKGLNVV
                     ELSRKIVRLIAKNGAEKIKLENLLKEKGLGKAKSLQIIAALEFGRRMRPDKKPEILSP
                     EDVWKLCGDLRASKKEHFAVFYLDTASALIERQIISIGTLNASLVHPREVFEPAIALR
                     AAGIIAAHNHPSGNVEPSQDDLAITKRLAEAGKIMGIGLTDHVILGKNEFLSFKNKGL
                     I"
     gene            2975..3256
                     /locus_tag="PKK37_01475"
     CDS             2975..3256
                     /locus_tag="PKK37_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81085.1"
                     /translation="MTNKNYKQKTDCGSIATDGESPMVWFVRDLNNRADRVKHLEFHI
                     ERGDYFAVLATIINIALRESDFEKDKIFSKLYEDLQYLQENYDIVKKKP"
     gene            3475..4860
                     /gene="aspS"
                     /locus_tag="PKK37_01480"
     CDS             3475..4860
                     /gene="aspS"
                     /locus_tag="PKK37_01480"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNR81086.1"
                     /translation="MIGQTPGLVGRNVRLCGWVQTVRAHGKLIFFDLRDASGVIQSVV
                     FPKAPFFDLAKEVRPEWVVEFCGIVNERPEKMKNDKISTGGVEIVIDELKVLSRAQTL
                     PIAVDGDGYEIGEDNRLKYRYLDLRRPRLRRNLVMRHKVVNHLRQFLSARNFVEVETP
                     ILTKSTPEGARDFLVPARLQKGSFYALPQSPQQYKQLLMVAGLERYFQVARCLRDEDP
                     RADRQAEHTQLDIEMSFADEAEILALAEEMFAGMVKGLFPQKKITKIPFPRLTYKEAM
                     ERYGTDKPDLRENKNDPDELAFCFITEFPMFEWLEGDKRWNAMHHPFTMPRENDPERI
                     KTEPEKILAWQYDMALNGFEIFSGSLRNTDMETFSAIFEVLGHSPEETRKQFPHYFEA
                     FSYGVPPHGGMAPGIDRLMAVLLGEPNIREVMAFPKTGDNRDLMLSSPSPVDAVQLEE
                     LGLTILKKKSC"
     gene            4862..5827
                     /locus_tag="PKK37_01485"
     CDS             4862..5827
                     /locus_tag="PKK37_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNR81087.1"
                     /translation="MIRPYISLPLPPKKRAKKRNSIARRWFGFAVALVFGGGVCWAID
                     CGQVKLENYLYAQISAPIREIVIVSPPFRPTMELEAKAALCSRASAAGREKMIFEKNA
                     DEILPIASLTKLMTARVVLENPEIYNPSRIVFISAAAAGQPDVPVSGNLFFGEIRTVR
                     ELLDLMLFYSSNDAAWALAELMGENEFVDAMNRAAGEIGLSDTEFFNATGLDLDDGRF
                     NYSTARDLLALSKNILEFHPEIFATTKTPGFYRTQNGIFDFALWEGQTLVGGKTGYTE
                     KAGGCMIAVFDGANGWRYIGVLLGSANAESRVAEMQKIVNFSNNY"
     gene            5836..6945
                     /locus_tag="PKK37_01490"
     CDS             5836..6945
                     /locus_tag="PKK37_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81088.1"
                     /translation="MTLFTFNIIRMLIFTAVTSIIAFLAAPRLIVFLRRIKFWKKNAR
                     QKTITGEEASVFHSLHKERETSVPRGGGLLIWASVLLVALFFSMVSAAPDWIWQNLGI
                     SAPWWFVLLDFLSREQVWLPLFALVVGSLIGLADDILTVYGGGKYIGGGITFKRRLLA
                     VAIIGLVGGWWFYEKLGYETMHIPLAGDFPGGIDIFIGWFYIPLFVIVMLASWAGGVI
                     DGLDGLAGGAFVSIFAAFALIAFSQGKADLATFCAIICGALFAFLWFNIPPAKFYMGE
                     TGALGLTSAMAVAAFLTDSVVVLPVIAGLLVMEAGSVIVQLAHKKILKRKLWLSTPIH
                     HHFEAIGWPAHSVTMRFWILGIIFAAVGVAIRLMG"
     gene            6985..8418
                     /locus_tag="PKK37_01495"
     CDS             6985..8418
                     /locus_tag="PKK37_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81089.1"
                     /translation="MHKINLRYLRKKYPEFVYESFNWSAKSGDLSANFLFLTGDREFC
                     PEISICGIDKKQIKKIGEEAISNFVFHLGLAEMPSYWKAVCAPKIVVNAGYLDKTQIK
                     FWQDLFKRGMGQFFFENKLPFIVPEFEINFKRPRKYPCPLVQKFSSRILAPMGGGKDS
                     LVTVELLRKARQKTMLFALNPNESLARVCAAAGGPCATVFRQIDPRLAVMKKEGFLNG
                     HTPFSSLLSFYSVAIATLFDCGRIAISQERSSNEGNVKYLKKEINHQYSKSFDFEKKF
                     QAYSKKYLAENVDYFSILRPLYELQIAMLFSRYPEYFPKFISCNNPFTTKNRQNKNIG
                     WCGECPKCLSVFVMLYPFVGEKQAIKIFGKNFFENKKLLPLMAQLLGEGEAKPFECVG
                     TFAETRVAFYLSLVAAKKEGRDLPALLQVFEKKFLPKYGDMKSKSGKLLLLWNKNHCL
                     PEKFEATLRAGLDDFCRHFPPDVDNFI"
BASE COUNT         2297 a         1845 c         2328 g         2263 t
ORIGIN      
        1 gagggcttgt tttttgccgg ttgcgtgcta gtttatcatt atggaaaatt catcttcgag
       61 atacaatccg aaagaaacgg agcaaagaat atataacgac tgggaaaaga agggatactt
      121 caatcccgac aaatccaacc cggggacaaa aaaagtcttt tcggtggttt tgccgccgcc
      181 caacgtgacc ggcacgttgc atatggggca cgcgcttaat atcaccatcc aagacgcgct
      241 ggtgcgctat catcgtatga agggcgagac caccgtttgg atgccgggaa gcgatcatgc
      301 cggtatcgga ctacaaagca cgctggagaa gaaactgcgc aaggagggcg taagccgttt
      361 tgatttggga cgtgatgagt ttattaaaaa agcgtgggag tggaaagaac tttatggcaa
      421 tattattttg aatcaaattc gcgccatggg cgcgtcttgt gattggtcgc ggacaaaatt
      481 taccatggac gaagattatg tcaacgcggt ggaaacggtt ttcattcact atcaccggca
      541 aggatgggtt taccggggtc agcgggtggt gaattggtgc ccgcgttgcg gcacttcaat
      601 ctcggatctg gagaccgact atgtcgccga agcgtcaaag ctctggcata taaaatatcc
      661 aatcgcttcg ttttcgccaa aaaccaaggc ggatgaaatc ggcgaatata ttatcgttgc
      721 caccacgcgg ccggaaacaa tgtttggcga taccgccgtg gcggtcaacc ccaaagacga
      781 gcgctatcgg gcgttaatcg gcaaaaaagc ggtcctgccg gttgtcggtc gggagatccc
      841 gatcattgcc gacaaatcaa ttgatattga ttttggcacc ggcgcggtca aaataactcc
      901 tgctcatgat ttaaacgatt ggcaaattgg ccaaaagcac aaattgccgc tgatcgcggt
      961 gattgacgag cgggggaaaa tgaacggcaa tactcccgcg gaatatcagg ggttgaaaac
     1021 cggcgaggca aggaataaat tggcggctca tttgcaagaa atcggtttat tggaaaagat
     1081 tgaagattat gagcaccaag tacccaaatg ctcccgatgc ggcgcggcga tacagctgat
     1141 tccgtcaatg caatggtttg ttaaaatgga tgatttggca agaaaagccc tgattgccgc
     1201 gaattcggga aaaataaaat tccatcccaa gcgctgggaa agcacctatc attcctggtt
     1261 gaaggagccg cgggactggt gcgtgtcgcg ccaattatgg tggggacagc gcttgccggt
     1321 ttggttttgc caaactgaac ccgataaata taccgttgcg aaaagcgaac cttcaaaatg
     1381 tccgatttgc gggaaatgcc ggcccaaaag gagcgaggat gtgtttgaca cttggttttc
     1441 ttccgccttg tggccgctgg cggtgtttgg ctggccaaaa attacccaag atttgaaaaa
     1501 attctatccg acttcggttt taaccacggg ccgcgatatt atcaacctct gggtggcccg
     1561 gatggcgttt tcgggaatgg agtttatgaa caaggcgccg ttcgctcaag tgattatcaa
     1621 cgcgatggtt ttgaccaaag acggcaaaag aatgtcaaaa tcgttgggta ccggcattga
     1681 tcccatgact ttaattgaaa aatacggcac cgacgccact cgtttcggtt tggtatggca
     1741 agtgagcgaa tcgcaggatt tgcattttaa cgaagacaat atgcttgccg gacaaaaatt
     1801 ctgtaacaaa atttggaatg cctcgcgatt tgtttgcggg cagattgagg gcgaaaagaa
     1861 tatatattcg cttaccgtaa aaccgaaacc gataacctcc gccgacaaaa aaattatggc
     1921 gggattggcg aaaatttgcc gatcaaccga aaaaaatatc aaccgttacg cattgggggc
     1981 ggcaatccgc gatctttatg attttttctg gcataaattt tgcgatgttt acattgaagc
     2041 ggcaaaaaat caaattcgcg gcgcgaaaaa caaaactcaa acccgaaaca cccaaaaaat
     2101 cctgatctat gttttggcga attccctcaa actcctgcac ccgtttatgc cgtttgtcac
     2161 cgaagaactc tggcagaccc tgcccctcaa aaccaaaacc gacctgatta tttccccctg
     2221 gcccaagtcc tagtttttct aaaaacattc caacattctg cagaatgttg gaatgttttt
     2281 tgtttttgtc tttaataatg gtaaaatttg cttatgaaga taagggactt ggcaaaagtt
     2341 gaccggccgc gggagaaatt ggaaaaatat ggcccggaaa aactggcaac cgctgaatta
     2401 atggcggtgc ttttggggtc ggggatcaaa ggattaaatg tggtggaatt gtcaagaaaa
     2461 atcgtgagat tgatcgccaa aaacggtgcg gaaaaaataa aattggaaaa tttgctcaaa
     2521 gaaaagggct tgggcaaggc aaaatcgttg cagataatcg cggcgttgga attcggccgg
     2581 cggatgcgcc cggataaaaa accggaaatt ttgtcgcccg aagacgtttg gaaattatgc
     2641 ggtgatttgc gcgcttccaa aaaggaacat ttcgcggtct tttatttgga cactgccagc
     2701 gcgctgattg aacggcaaat aatctccatt ggcacgctca acgccagctt ggtgcatccg
     2761 cgcgaagtgt ttgaaccggc gattgctctt cgcgccgcgg gaataatcgc ggcgcacaat
     2821 catcccagcg gcaatgttga gccctcccaa gatgacttgg cgattaccaa aagattggcc
     2881 gaagccggca aaattatggg tatcggatta accgatcacg tcattcttgg caaaaatgaa
     2941 tttttaagtt tcaaaaataa aggattaatt taaaatgact aacaaaaatt ataaacaaaa
     3001 aacggattgc ggatcaatcg cgacggacgg ggagtcgcca atggtttggt ttgttcgcga
     3061 tttaaacaat cgcgccgatc gcgtcaagca tttggaattt catattgagc gcggcgatta
     3121 tttcgcggtt ctggcgacaa tcattaacat cgctctcaga gaatcggatt ttgagaaaga
     3181 taaaatattt tccaaactct acgaagatct ccaatacctc caagaaaact acgatatcgt
     3241 caaaaagaaa ccataaaccg ttcgttcgca tattttcctc tgccaaaaca atggaatggt
     3301 tcgcccgaag ggtggaatgg tttggcgggg ggcggtgtcg gggagatggc ggggcgttga
     3361 aaaatggggg gaaatggagt aggatgatgg ggtagaacga caaagtggaa ttaatttatt
     3421 cattgaaaca attatattga tactatggat atggaaaaac aattgccgag aacgatgatt
     3481 ggccaaacgc ccgggcttgt cgggcggaat gtgcgcttgt gcggctgggt gcagacggtg
     3541 cgcgctcatg gcaagctgat tttttttgat ttgcgtgatg ccagcggggt gatccagtcg
     3601 gtggtctttc cgaaggcgcc gttttttgat ttggccaaag aagtgcgtcc cgagtgggtg
     3661 gtggaatttt gcggtattgt taacgagcgc ccggaaaaaa tgaaaaacga caagatctcc
     3721 accggcggcg tagagattgt cattgacgag ctgaaagttt tatcaagggc gcaaacgtta
     3781 cctattgccg ttgacggtga cggttatgag atcggtgaag ataaccggct caaataccgc
     3841 tacttggatt tgcgccgtcc gcgcctgcgc cgcaatctgg tgatgcgcca taaagtggtc
     3901 aatcatttgc gccaattttt gtccgcgaga aattttgtgg aagtggaaac gccgatattg
     3961 accaaatcca ctcccgaagg cgcgcgcgat tttctggtac cggcgcgctt gcagaaagga
     4021 agtttttacg ctttgccgca aagcccccaa caatacaaac aattgctgat ggtcgccgga
     4081 ctggaacgtt attttcaagt ggcgcgctgt ttgcgcgatg aagacccgcg ggcggaccgg
     4141 caagcggagc acacgcaatt ggatattgag atgtcgtttg ccgacgaagc ggaaatttta
     4201 gctttggccg aagagatgtt cgcgggaatg gtaaaaggac tgtttccgca aaagaaaatt
     4261 accaaaattc cgtttccccg tttgacgtat aaggaagcga tggagcgata cggcaccgac
     4321 aagcccgatc tgcgcgaaaa caaaaatgat cccgacgaac tggcgttttg cttcatcacc
     4381 gaatttccga tgtttgaatg gcttgaaggg gacaagagat ggaacgcgat gcatcatcct
     4441 tttactatgc cgcgggaaaa cgacccggaa cgaataaaaa cggagccgga aaaaattttg
     4501 gcgtggcaat atgacatggc gctgaacggc tttgaaatat tctcgggcag tttgcgcaat
     4561 accgatatgg aaactttttc ggcgattttt gaagttttag ggcactcgcc ggaagaaacc
     4621 agaaaacaat ttccgcatta ctttgaagcg ttttcctatg gcgtgccgcc acacggaggc
     4681 atggcgccgg ggatagatcg tctgatggcg gttttactgg gcgaaccgaa tatccgcgaa
     4741 gtgatggcat ttccaaaaac gggggacaat cgcgacttaa tgctaagctc tccatcgccg
     4801 gttgacgcgg ttcaattgga agaactcggt ttgacgattc ttaaaaagaa aagttgttga
     4861 aatgatcagg ccatatattt ctttgccatt accgcccaag aagcgggcga aaaaacgaaa
     4921 ttccattgcc agaaggtggt ttggctttgc tgtcgctttg gtctttggcg gcggagtctg
     4981 ctgggcgatt gactgcggcc aggtgaagct ggaaaattat ctctacgcgc agatctccgc
     5041 gccgataaga gagatagtga ttgtttcacc gccattcagg cccacaatgg aactggaagc
     5101 taaagcggcg ttgtgttctc gcgccagcgc ggcgggaaga gaaaaaatga tttttgaaaa
     5161 aaacgccgat gaaatattgc ccattgcttc attgacaaaa ctgatgactg ccagagtggt
     5221 tttggaaaat cccgaaattt acaatccttc aagaattgtt tttatcagcg ccgcggcggc
     5281 cggacaaccc gacgtgccgg tttccggcaa tctgttcttc ggggaaatcc gcaccgtcag
     5341 agaattgctt gatttgatgc ttttttattc cagcaacgat gcggcttggg cgctggcgga
     5401 attaatgggc gagaacgaat ttgtggacgc gatgaaccgc gccgccggcg agattgggct
     5461 ttcggacacc gaatttttca atgccaccgg attggatttg gacgacggcc ggttcaatta
     5521 ttccacggcg cgggatttgc tcgcgctctc caagaatatt ttggagttcc atcctgaaat
     5581 ttttgccact accaaaaccc cgggatttta ccgcacgcaa aacggaattt ttgattttgc
     5641 gctatgggaa ggccaaactt tggttggcgg taaaaccggc tataccgaaa aagcgggggg
     5701 atgtatgatt gcagtgtttg acggcgcgaa cggatggcgc tacatcggcg ttttgctggg
     5761 atcggcgaac gccgaaagcc gtgtggccga aatgcaaaaa atcgtgaatt tcagcaataa
     5821 ttattagcga tttttatgac attattcact ttcaatatca tccgcatgct gatttttacc
     5881 gcggtcactt ccatcatcgc gtttctggcc gcgcctcgtt tgatcgtttt tttgcgccgc
     5941 attaaatttt ggaaaaaaaa cgctcgccaa aaaaccatca ccggcgaaga agcttcggtt
     6001 ttccattctc tgcacaaaga gcgggaaact tcggtgccgc gcggtggcgg attgttaatc
     6061 tgggcatcgg tattgctggt ggcgctgttc ttttcaatgg tttctgcggc acccgattgg
     6121 atttggcaaa atttgggcat cagcgcgccg tggtggttcg ttttacttga ttttttatcg
     6181 cgcgaacaag tttggcttcc gctgtttgcg ctggtggtgg gatcactgat cgggctggcc
     6241 gacgatattt taaccgttta cggcggcggc aagtatatcg gcggcggcat caccttcaag
     6301 cgccgcctgc tggcggtggc gatcatcgga ttggtgggcg gctggtggtt ctacgagaaa
     6361 ctgggctatg aaacaatgca tattcctttg gcgggcgatt ttcccggcgg cattgatatt
     6421 ttcattggct ggttctacat tccgcttttt gtcattgtga tgctggcctc ctgggccggc
     6481 ggcgtgattg acggcctgga cggtttggcc ggcggcgcgt tcgtttccat ctttgcggcg
     6541 tttgcactga ttgctttttc gcagggaaaa gcggacctgg cgactttttg cgcgattatt
     6601 tgcggcgcgc tgttcgcgtt tttgtggttt aatattccgc cggcaaaatt ttatatgggc
     6661 gaaaccggcg ctttgggact cacttcggcg atggcagtgg cggcattcct gacggattcg
     6721 gtggtggtgc taccggtaat cgcgggattg ctggtgatgg aggcgggatc ggtgattgtc
     6781 cagttggcgc acaagaaaat cttgaaaaga aaactctggc tttccacgcc gatacatcat
     6841 cattttgagg cgattggctg gccggcgcac agtgtgacga tgcgtttttg gattttggga
     6901 attatttttg ccgcggtggg ggtggcgatc cgtttaatgg ggtaaggggt tgaatattcc
     6961 actgaagata ccatccataa actgatgcat aaaattaatt taaggtactt gcgaaaaaaa
     7021 tatccggaat ttgtttatga aagctttaat tggagcgcga agagcggaga tttgtccgcg
     7081 aattttttgt ttttgacggg cgatcgggaa ttttgtccgg agatttccat ttgcggcatt
     7141 gataaaaaac aaatcaaaaa aatcggggaa gaagcaatct ctaatttcgt gtttcacttg
     7201 ggattggccg aaatgcccag ctattggaaa gcggtttgcg cgcctaaaat tgtcgtcaac
     7261 gccggctacc tggacaaaac gcaaatcaaa ttttggcagg acttatttaa acgggggatg
     7321 gggcaattct tttttgagaa caagttgccg tttatcgttc ccgaatttga aatcaatttc
     7381 aaaaggccgc gaaaatatcc ttgtccgctt gtgcaaaaat tttcttcccg cattttggcg
     7441 ccgatgggcg gcggcaagga ttcgctggtg acagttgaat tgttgcgaaa agcgcggcaa
     7501 aaaacgatgc tgttcgcgct taatcccaat gaatcgttgg cgcgggtctg cgcggccgcc
     7561 ggcggtccgt gcgcaacggt tttccggcag attgacccgc gtctcgcggt gatgaaaaag
     7621 gaaggatttt taaacggaca cacgccgttc tcctcgttgt tgtcgtttta ttccgttgcc
     7681 attgccacgc tttttgattg cggccgtatt gccatttccc aagagagaag ttccaacgag
     7741 ggcaacgtca aatatttaaa aaaagagata aaccatcaat attccaagtc gtttgatttt
     7801 gaaaagaaat tccaggcata ttcaaaaaaa tatctggcgg aaaacgttga ttatttttca
     7861 atcctgcggc cgctttatga attgcaaatc gcgatgctgt tttcgcgata tcccgaatat
     7921 tttccaaaat ttatcagttg caacaacccg tttacgacaa aaaaccggca gaataaaaat
     7981 atcggctggt gcggcgaatg tcccaaatgt ttgtcggtgt ttgttatgct ttatcccttt
     8041 gtcggcgaaa aacaagcaat aaaaattttc ggcaaaaact tttttgaaaa caaaaaactt
     8101 ttgccgttga tggcgcagtt gttgggagaa ggggaggcga agccgtttga gtgcgtgggc
     8161 acgtttgccg aaacccgcgt ggcgttttat ttgtcgctgg ttgccgcaaa aaaggaaggt
     8221 cgggacttac ctgctttgtt gcaagtattt gaaaaaaagt ttttgccgaa gtatggcgat
     8281 atgaaatcaa aaagcggcaa gcttttgtta ttgtggaata aaaatcattg tttgcccgaa
     8341 aaatttgaag ctacgcttcg cgccggcctt gacgattttt gtcggcattt cccgccggat
     8401 gtggataact ttatttgaca tattctgggg aaggcataaa atgatactgt cggtgaggga
     8461 gaatgcgggc gaaaggaagg tacattaaaa acggaagatt cgaaaatgat ttttttcaaa
     8521 agagcggatt ttttgaaaga aacaaaatta ttttcggaat aatctctttt atggggacgg
     8581 cgatcccgcc ctatcaagca aggagagctc tgagaagcgt tttcgccaag cgttccgaac
     8641 cggtcttcgg gatgattgag ggaaacgttt ccacctctcc ttttttttgt tggtattcgg
     8701 cttattttta tgataaaata ttacggtatg aag
//
LOCUS       DAOQWE010000023         8296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_9087_length_8296_cov_7.483436, whole genome shotgun sequence.
ACCESSION   DAOQWE010000023 DAOQWE010000000
VERSION     DAOQWE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9087_length_8296_cov_7.483436"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1419)
                     /locus_tag="PKK37_01500"
     CDS             complement(220..1419)
                     /locus_tag="PKK37_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81090.1"
                     /translation="MENIKKRILFNDLVKYFGNKEALVITGMRQVGKTTLMRQVFDWA
                     GDCPKLWFDFENPLDVKFFEDIDYNNVYKNLEKTAKKSARSRLTVFIDEIQIYPETTK
                     IIKYLIDHYRVKFIVTGSSNYYLKNLFPESLSGRKFLFELPTLSFREYLYFKGKIEEK
                     EALTGEISKALRNSDIFNHKKFEADYDDFVHYGGFPAAVLETDFDVKKQILKNIFSSF
                     FEKDLKILSDYKDIQELRDLLLLLAPRIGSLIDVTRLASELGTNRPKIYHYLEFLQGA
                     FVIKLLPRYAKTIDKAVAGGRKAYFSDTGLLQVMGGVNDAQLFENAVINQLFGYGKVS
                     FYNKRNTAEIDAILDKKIAFEIKLTGIPKDVLRLQKLAADVKIPAQYVISKKFAKGKN
                     FISPVVL"
     gene            complement(1485..1619)
                     /locus_tag="PKK37_01505"
     CDS             complement(1485..1619)
                     /locus_tag="PKK37_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81091.1"
                     /translation="MAKFRNVYRWSARHKAKTVIKEETPGITLIKSDSKEEKAKIQAT
                     "
     gene            complement(1722..3038)
                     /locus_tag="PKK37_01510"
     CDS             complement(1722..3038)
                     /locus_tag="PKK37_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81092.1"
                     /translation="MDKNELVKILDDWNFWNKKLDTGIARKIYLDKLKKALPSEQVKV
                     IIGARRSGKSYIMRQFAWHLIEKENVNKDNILMVNFEDPRFAELDTKLLDRLFETYLE
                     HRSPRGKIYLFLDEIQEVPRWEKWVRAAQELKKADMIISGSNAKLLSGELATVLTGRH
                     LDITVMPLSLREFLDFKGLGAGNAAEIIKNEIRINSLLEEFMRFGSFPLVVLGQEREK
                     ILLSYFDDVVNRDLVRRYKIRKTEEIKKLIKFYMSNISSPVTFTSSGKFLGISAQSAE
                     RFSAYLEASYLTFFVKRFSFKFKEQEKSPRKVYAIDTGLANIAGFQFSPNRGKLAENA
                     VFLELKRKAHSNPRMEIYYWKSLAGEEVDFVIKEDASINQLIQVCWDARNAQTKNRET
                     KSLVKAMAEFESDNGIIITGNYKNEEIVNGKKIKYIPLGEWLLASC"
     gene            complement(3485..3901)
                     /gene="rpsI"
                     /locus_tag="PKK37_01515"
     CDS             complement(3485..3901)
                     /gene="rpsI"
                     /locus_tag="PKK37_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNR81093.1"
                     /translation="MKEPAAKKTEKYYEAVGRRKTATARVRLYTKGDKEFMVNGHPFG
                     LYFKGAEFKNVVESPLVKMNCTDRFRVTAVVKGGGVNAQAEALRHGITRALVLFNADF
                     KKRLKKSGYLTRDPRMKERKKPGLKKARRAPQWAKR"
     gene            complement(4041..4400)
                     /gene="rplM"
                     /locus_tag="PKK37_01520"
     CDS             complement(4041..4400)
                     /gene="rplM"
                     /locus_tag="PKK37_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNR81094.1"
                     /translation="MISTKRETHTLDASGIALGKLAANVAVILRGKNKPGFVKNVDAG
                     DFVVVKNFAGVKITGRKFEERKYYRYSGHIGGLNETPLPKLMKENPAEVLRRAVYRML
                     PDNKLRDKMIKRLTVKQ"
     gene            complement(4417..4881)
                     /gene="rplQ"
                     /locus_tag="PKK37_01525"
     CDS             complement(4417..4881)
                     /gene="rplQ"
                     /locus_tag="PKK37_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNR81095.1"
                     /translation="MYKRIKGKKFSRKTDQRRAFMKSLAVNLVMQEKIRTTKIRARQA
                     ASMVEKLITKAKKGDLASRRALVSVLNPAAAEKLVKSIAPRFKDRVGGYTRVIKIAPR
                     VGDGAPMAMVELLDRPAEKTPAAPESGKKAEKKNKAEKPKKEKTAKPKKEEK"
     gene            complement(4906..5595)
                     /locus_tag="PKK37_01530"
     CDS             complement(4906..5595)
                     /locus_tag="PKK37_01530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNR81096.1"
                     /translation="MIPLPLTPKVVERKGNSALIEIEALYPGYGITVGNALRRVLLSS
                     LEGAAVTQVNIKGVPHEFSTIPGISEDAIALMINIRQLRFSIVGDEPQTCVLKAKGEK
                     EVTGADLELPSQLTLANPGVHIADLTSKTAKLEMEIKVEKGTGYLSKESRMGKEKLEI
                     GAIAIDAMFTPVKKVGFKTQNMRVGDRTDFDKVIFDIETDGTITPETAFSRASEILVN
                     HFDMFKNAFTA"
     gene            complement(5781..6413)
                     /gene="rpsD"
                     /locus_tag="PKK37_01535"
     CDS             complement(5781..6413)
                     /gene="rpsD"
                     /locus_tag="PKK37_01535"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNR81097.1"
                     /translation="METNVKCKICRRAGAKLFLKGARCYNQKCAMVRRATTPGKKGKR
                     RPRSLSEYGRELREKQRMKAWYSLREQQFKNYVKAILEQKGRNADLPLALVDSLESRL
                     DNVVFRLGFAPSRLAARQLVSHKSFLVNGKPVNLPARRINKGDVVSVVARKASNAYFT
                     QLKPALKSQKPPLWLSLDADKIEGKRVGDPTLEEAMPPAEIGSVFEFYAK"
     gene            complement(6428..>6880)
                     /gene="rpsK"
                     /locus_tag="PKK37_01540"
     CDS             complement(6428..>6880)
                     /gene="rpsK"
                     /locus_tag="PKK37_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNR81098.1"
                     /translation="MGKKHVAVQNKEDLLKERDAVEAKVKKEYTVKNPARARQGKVFI
                     SSTYNNTIVSFADEGGNVLCAKSAGSIGFKGTKKGTPFAASKVAEAVAEIIKKFSVER
                     VKVIVKGIGSGRDSAIRSLVARGIDVAAIKDVTPVPHNGCKPRKPRKV"
     assembly_gap    6881..6980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6990..7379)
                     /gene="rpsM"
                     /locus_tag="PKK37_01545"
     CDS             complement(6990..7379)
                     /gene="rpsM"
                     /locus_tag="PKK37_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNR81099.1"
                     /translation="MPRIIGVNIPDNKQIGIALGYIYGIGRSSAKVVLAEAKIDPATK
                     AKDLSPEQMKLIEEIIKKRYHVEGDLKREIMMNVKRLKDIVSWRGMRHSRNLPVRGQR
                     TKTNTRTVRGNVRKTAGSGRKPAASPT"
     gene            complement(7470..7586)
                     /gene="rpmJ"
                     /locus_tag="PKK37_01550"
     CDS             complement(7470..7586)
                     /gene="rpmJ"
                     /locus_tag="PKK37_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNR81100.1"
                     /translation="MKVRASVKKICKDCQIVKRSRRVYVICKKNPKHKQKQG"
     gene            complement(7637..7864)
                     /gene="infA"
                     /locus_tag="PKK37_01555"
     CDS             complement(7637..7864)
                     /gene="infA"
                     /locus_tag="PKK37_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNR81101.1"
                     /translation="MTKQRDPRFQGQVFESLPDAKFRVRLSDGREVLCHLAGKLKMFR
                     IKILPGDNVTVEMSPYDNARGRIVYRGKAAN"
     gene            complement(7938..>8296)
                     /locus_tag="PKK37_01560"
     CDS             complement(7938..>8296)
                     /locus_tag="PKK37_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81102.1"
                     /translation="AIAYRKADLDALGKEFVSGQIPEGMICEESSFKVQYESAGIDKE
                     QKLPSFDLAVSATIYAGMHPEDLKKGLSEKKSEEAKMFLMSRPGTKKAEIRLSPPWRF
                     NVPRDLDRIDIETILE"
BASE COUNT         1997 a         2150 c         1698 g         2351 t
ORIGIN      
        1 gtgatgcgca aaattttcgg cggttcccgc tcggttgacg gcgccaaggc gcacgaggtc
       61 aacacttcgg tgatcgaaac ccaattgcgc cagaatcccg gccaatcatt cttcagggtc
      121 atgttgccgc ttatcaatca acgccgctca aaattgtgag cagttacaaa gccgccccga
      181 acggggcggc ttgaaaaatt tttaaaccga taagatgctt cataaaacca ccggtgaaat
      241 gaaatttttg cctttggcga atttcttgga aatcacgtat tgcgccggta ttttgacgtc
      301 cgcggccaat ttttgcaatc gcaaaacatc tttgggaatg ccggtaagct tgatttcaaa
      361 agcgattttt ttatcaagaa tggcatcaat ctcggcggtg ttgcgcttgt tataaaaact
      421 aaccttccca tatccaaaca actgattaat cactgcgttt tcaaacaatt gagcatcatt
      481 aacgccgccc atcacctgca ataatccggt atcgctaaaa tatgcctttc ggccgcccgc
      541 aaccgccttg tcaatggttt ttgcgtagcg aggcaataat tttatgacaa aagccccctg
      601 caaaaattcc aaataatggt atattttggg gcggtttgtc cccaactctg aagccaacct
      661 ggtaacatct ataagcgatc cgatccgcgg cgccaaaagc aacaataaat cacgaagctc
      721 ttgaatgtct ttatagtcgg ataatatctt taaatctttt tcaaaaaaag aggaaaaaat
      781 attttttaaa atttgttttt taacatcaaa atcggtttcc aacaccgccg cgggaaatcc
      841 tccataatgg acaaaatcat cataatcagc ttcaaatttc ttgtgattga aaatatcgct
      901 gttgcgcaaa gcttttgata tttcaccggt taacgcttct ttttcttcga ttttgccctt
      961 gaaatacaaa tactcccgaa aactaagagt tggcaattca aaaagaaatt ttctacccga
     1021 aagcgactcg ggaaataaat tttttaaata ataatttgat gatccggtaa caataaattt
     1081 aacgcggtaa tgatcaatca aatatttgat aattttagtt gtctccggat aaatttggat
     1141 ctcgtcaatg aaaaccgtca atctcgatcg agcggatttt ttagccgttt tctccagatt
     1201 tttatagaca ttattatagt caatatcttc aaagaacttt acatccagcg gattttcaaa
     1261 atcaaaccac aacttggggc agtcgcccgc ccagtcaaaa acctggcgca tcagggtggt
     1321 tttgcctacc tgcctcatac cggtgatcac caacgcctct ttattgccaa aatatttcac
     1381 caaatcattg aataaaatcc ttttcttaat gttttccata gtgtaataaa ataattacaa
     1441 aaaagcgtaa atatgtaata attatattac actctgaaaa caaatcaagt ggcttgaatt
     1501 ttcgcttttt cttccttgga atcgcttttt atcagagtaa tccctggagt ttcttcctta
     1561 atcacggtct tggctttgtg ccttgccgac caccgataaa cattgcggaa tttggccaaa
     1621 actccggcaa agggccggga accagtgtgg caacgctccc aaatcaagga gcaatgttgc
     1681 ttcattcccc ggcccccccg ccagtagctt tttgagcccg atcaacaaga agcaagtagc
     1741 cattctccaa gcggaatata tttaattttt ttgccattca cgatttcctc gtttttgtaa
     1801 tttccggtta ttattatgcc attgtccgat tcaaattccg ccatggcttt aaccagtgat
     1861 ttagtttccc ggtttttcgt ctgcgcgttg cgcgcgtccc aacaaacttg gatcaattga
     1921 tttattgaag catcttcttt tatcacaaaa tccacctctt ctcctgccag gcttttccaa
     1981 tagtatattt ccatacgcgg attggaatga gctttccttt taagctccag aaataccgca
     2041 ttttccgcca acttgccgcg gttggggctg aattggaatc ccgcgatgtt tgccagaccg
     2101 gtgtctatgg cgtagacttt tctcggactt ttttcctgtt ctttgaattt gaacgaaaat
     2161 cttttgacaa agaatgtcaa ataggatgct tccaaataag ccgagaacct ttccgccgat
     2221 tgcgccgaaa tccccaaaaa cttgcccgaa gaagtaaaag tgacgggaga agatatattg
     2281 ctcatataaa attttatcag cttctttatt tcttcggttt ttcttatttt ataccgcctt
     2341 accaaatccc gattaacgac atcgtcaaaa tacgaaagca gaattttttc cctttcctgc
     2401 cccaatacca ccagcggaaa agaaccgaat ctcataaatt cttcaagcaa cgaatttatc
     2461 cgaatttcat tttttataat ctcggcggca ttgccggcgc ccaaaccttt aaaatccaaa
     2521 aattcgcgaa gcgaaagcgg cataaccgtg atatccaagt gtctgcccgt aagcaccgtt
     2581 gccaattcgc cgctaagcaa tttcgcgttt gagcccgaaa tgatcatgtc ggcttttttt
     2641 aattcctgcg ccgcacgcac ccatttttcc cagcgcggca cttcttggat ctcatcaaga
     2701 aacagataaa tcttccctct tggagaacga tgctccaaat acgtttcaaa caaccggtca
     2761 agcaatttcg tgtcaagttc cgcaaaacgc gggtcttcaa aattgaccat caaaatatta
     2821 tccttgttca cattctcttt ttcgatcagg tgccaggcaa actgtctcat tatatatgac
     2881 ttgccactgc gccgcgcgcc aatgataact ttcacttgtt cgcttggcag agcctttttc
     2941 aatttgtcca aataaatttt tcttgcaata cccgtatcca attttttatt ccaaaaattc
     3001 caatcatcaa gtattttcac aagttcattc ttatccatta attcaagcat atgttataaa
     3061 attatattat tttatcacat atatatttta tatcattcaa aataatcgtc aagtgcaacg
     3121 aaccgcaaga cgaaaaaaca ccgccggctt atgccgacag tgtttttgtc aatttccccg
     3181 aatgtaacca ctcactgggg tagcgtattt ttgcatttta agggtctatc ccccgtgcct
     3241 gtcccgaggt aatccgaggg ataatcgcgg gggttagacc ctggcaaaga attgcaagca
     3301 caaaattaaa agggtctaac cctcgcagta acgagggata gaccctttta attttaaact
     3361 cggtgagtgg ttaccaaggg tatagacagg aaaaaaacca aaaagccgcc ccgttcgggg
     3421 cggctttctc tgtatttgaa ttcttctaat aacaactatt aactataaac tattaactgg
     3481 cgcgttagcg cttcgcccat tgaggggcgc ggcgggcttt cttcaagccg ggtttcttgc
     3541 gttccttcat ccgcggatca cgggtgaggt agccggattt tttcaggcgt ttcttgaaat
     3601 cggcgttaaa cagcaccagc gcgcgggtaa tcccgtggcg caaggcttcg gcctgagcgt
     3661 tgactccccc gcctttgacc actgcggtaa cgcgaaaacg gtcggtgcaa ttcattttca
     3721 ccagcggcga ctcaaccacg tttttgaact ccgccccctt gaaataaagg ccgaacggat
     3781 ggccgttaac cataaattct ttgtcgccct tggtgtagag acgcacgcgc gcggtcgcgg
     3841 tcttgcgccg ccccacggct tcataatatt tttccgtttt cttcgccgcc ggttctttca
     3901 cggcaacaac tgccggcgcg gccgcggcgg gcttcgccgc cggttttttg atcaccgcgg
     3961 gcttgcttac cgccggtttt ttggccgcaa ctttcacggc aggcttcgcc gccggttttt
     4021 tttcaatggt ttctgccata ttattgttta acggttaacc gcttgatcat tttgtcgcgc
     4081 aatttattgt cgggcagcat gcgataaacc gcccggcgca aaacttcggc gggattttct
     4141 ttcatcagtt tcggcaaagg cgtttcgttc aatccgccga tgtggccgct gtagcggtaa
     4201 tatttgcgct cttcaaattt tctgcccgtg attttgacac cggcaaaatt cttgaccacg
     4261 acaaaatcgc cggcgtcaac attcttgacg aatcccggct tgttttttcc ccgcagaatc
     4321 accgcaacgt tcgcggccag cttccccagg gcaattccgg aagcatcaag ggtatgggtt
     4381 tctcttttcg tggaaatcat attggctcgt tttagcttat ttctcttctt ttttcggctt
     4441 ggcggttttc tcttttttgg gtttttccgc tttgtttttc ttctccgctt ttttgccgga
     4501 ttccggcgcg gcaggcgttt tttcggccgg ccggtcaagc aactccacca tcgccattgg
     4561 cgcgccgtcg ccgacgcgcg gcgcaatctt gatcacccgc gtgtatccgc cgacacgatc
     4621 cttgaaccgc ggagcgattg atttgaccag cttttcggcc gcggcgggat tcaaaacgga
     4681 taccagcgcg cggcgcgacg ccaaatcgcc cttcttagcc ttggtaatca acttttccac
     4741 cattgacgcg gcctggcggg cgcgaatttt cgtggttctg atcttttctt gcatcaccag
     4801 attgaccgcc agcgatttca taaacgcccg gcgctgatcg gtttttcggc tgaatttttt
     4861 gcccttgatt cttttgtaca tagaaagtta attcaaaatt caaaatcatg cggtaaaagc
     4921 gttcttgaac atatcaaagt gattcaccaa aatctcggac gcccttgaaa acgcggtctc
     4981 cggcgtgatc gtgccatcgg tctcaatgtc aaaaatcact ttgtcaaaat ccgtgcgatc
     5041 ccccacgcgc atattttgcg ttttgaatcc cacttttttc accggcgtga acatcgcgtc
     5101 aatcgcaatc gcgccgatct ccaatttttc tttgcccatc cgcgattcct ttgaaaggta
     5161 gcccgtccct ttttccacct tgatttccat ttccagcttc gccgttttgg aagtaagatc
     5221 ggcaatatgc acgccgggat tggccaaagt gagctgggag ggcaattcca gatccgctcc
     5281 ggtgacttct ttttccccct tggctttcaa cacgcaggtt tgcggctcgt cgccgacaat
     5341 ggaaaacctc aattggcgga tgttgatcat caatgcaatc gcgtcttcgg aaattccggg
     5401 aatggttgaa aactcatgcg gcaccccctt gatattgacc tgggtcaccg ccgcgccttc
     5461 cagcgaagag agcagaaccc ggcgcaaagc gttgccgacg gtgatgccat atccgggata
     5521 aagggcttca atttcaatca aggccgaatt tccttttctt tcaactactt ttggggtcaa
     5581 aggcaatgga atcatattcc aaattaaaat ttaaaatata aaagttaaaa attaaagctt
     5641 atattttaaa ttttaagaag ttaagccgta ggatatcgag cgtaattcca tcctgcccgc
     5701 gcccatatcc tcaacgactg caatcaaatg ttttaatcaa ctaccatcta taattaataa
     5761 tttataactg ataatcaatt ttatttggcg tagaattcaa agacggaacc gatctccgcc
     5821 ggcggcattg cctcttccag cgtgggatcg cccactcttt ttccctcaat cttgtccgcg
     5881 tccaaagaca gccataacgg cggtttttgg gacttgagcg cgggtttcaa ctgagtgaaa
     5941 taggcattgc tcgctttgcg cgccaccacc gacaccacat ctcccttgtt aatccggcgc
     6001 gccggcaaat taaccggctt gccgttgacc aagaaagact tatgactcac cagctggcgg
     6061 gccgccaaac gcgagggagc aaatcccagg cggaaaacca cgttgtccaa acggctctcc
     6121 aaggaatcaa ccagcgccag cggcaaatcc gcattccggc ctttctgctc caaaatcgcc
     6181 ttgacataat tcttgaattg ctgttcgcgc agactgtacc acgctttcat ccgctgtttt
     6241 tcccgcagtt cgcggccata ttcagacaac gaacgaggac ggcgcttgcc tttcttgccg
     6301 ggggtcgtcg cccggcgcac catcgcgcat ttttggttgt agcaacgggc accctttaag
     6361 aacagcttgg cgccggcgcg ccggcaaatt ttgcatttaa cgttggtttc cattgaagaa
     6421 tgattaatta aactttgcgg ggtttgcggg gtttgcatcc gttgtgcggg accggcgtga
     6481 catccttgat cgcggccacg tcaattcccc gcgccaccag cgaccgaatc gccgaatcgc
     6541 gcccggagcc gatgcctttc actatcacct tgacccgttc caccgaaaac ttcttgatga
     6601 tctcggccac cgcttccgcc accttggagg ccgcaaacgg cgtccctttc ttggtgccct
     6661 tgaagccgat gcttcccgcg gatttggcgc acaaaacatt gcccccttcg tcggcgaaag
     6721 aaacaatcgt gttattgtag gtggaagaaa taaaaacctt cccctggcgc gcccgcgccg
     6781 gattcttgac cgtgtactcc ttcttgacct tggcttcaac cgcatcccgc tctttcagca
     6841 ggtcttcctt attttgaacg gcaacatgct tcttacccat nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn ttttaatttt tatgtcgggc tggcggccgg cttgcggccg
     7021 gaacccgcgg ttttgcgcac attgccccgc accgtgcggg tattggtctt ggtgcgctgg
     7081 cctctcaccg gaagatttcg cgagtggcgc attcccctcc aggaaacgat atctttcaag
     7141 cgcttgacat tcatcataat ctcccgcttc aaatcgcctt ccacatggta gcgcttcttg
     7201 ataatctctt caatgagttt catttgttca ggcgacaaat ccttggcttt cgtcgccgga
     7261 tcaatcttgg cctccgccag caccactttc gccgaagatc ttccgatccc gtaaatatat
     7321 cccagtgcaa tcccgatttg cttgttgtcc gggatgttaa ccccgattat ccgtggcata
     7381 tttcaaatta aaaattaaaa tcaaaaaatt aaaaacaaaa aaactgtgcg cgccatccct
     7441 tgtgcgcatc gcaatgccga ccctcgcggc tacccctgtt tttgcttgtg cttgggattt
     7501 tttttgcaaa taacgtaaac tcgccgcgaa cgcttcacga tttggcaatc cttgcaaatt
     7561 ttcttgaccg aagctcttac tttcatattt tttaaccgat aatcttcatt ttgccgacaa
     7621 gactcgcccg caagcgtcag ttggcggctt tgccgcgata aacgattctt ccgcgggcgt
     7681 tgtcgtaggg actcatttcc accgttacgt tgtcgccggg aagaattttg atgcgaaaca
     7741 ttttcaattt tcccgccaga tggcaaagca cttcccgccc gtcggaaagc ctgacgcgaa
     7801 actttgcgtc cggcaacgat tcaaaaactt ggccttgaaa tctgggatct ctttgttttg
     7861 tcattggcac aattttaaat tctagcagat aacggtttga aagtcaaccg cccgacagct
     7921 ccgcggaaga aaacggttta ttccaagatt gtctcaatat caatccgatc caaatctcgc
     7981 ggcacattaa accgccatgg cggcgacagc cggatctcgg cctttttggt gcccggacgg
     8041 ctcatcaaaa acattttcgc ttcttcggat tttttttcgg aaagtccttt ttttaaatct
     8101 tcgggatgca ttcccgcgta tattgtcgcc gacaccgcca aatcaaacga gggtagcttt
     8161 tgttctttgt caatgccggc gctttcatat tgaactttaa aactgctctc ctcgcaaatc
     8221 attccctcgg gaatctggcc ggaaacgaac tctttgccca gtgcgtcaag atcggccttc
     8281 cgataggcga tcgctt
//
LOCUS       DAOQWE010000024         7990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_9505_length_7990_cov_8.037555, whole genome shotgun sequence.
ACCESSION   DAOQWE010000024 DAOQWE010000000
VERSION     DAOQWE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9505_length_7990_cov_8.037555"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(697..770)
                     /locus_tag="PKK37_01565"
     tRNA            complement(697..770)
                     /locus_tag="PKK37_01565"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Gly,seq:ccc)
     gene            complement(873..1277)
                     /locus_tag="PKK37_01570"
     CDS             complement(873..1277)
                     /locus_tag="PKK37_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNR81103.1"
                     /translation="MAVKIPKIKKRKSFGARLTALAMGLTALAIIAFLAFSNWRIWNN
                     RRDLDANIKDLQNQVQILEEHRRTLQAGLEAVQGESFQEERLREQGYKKPGEEVIAIL
                     RDPATSATNQEGGVSAANSFWADLWKKIKNIK"
     gene            complement(1526..>2188)
                     /locus_tag="PKK37_01575"
     CDS             complement(1526..>2188)
                     /locus_tag="PKK37_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018161.2"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HNR81104.1"
                     /translation="GRGAEKTIYTTGDWPDPTAIKDTREPWVKLEIVTPIDYLGPISD
                     LAKEYAAIYVNTDYVGNEKLLLAYEIPLRKIVSGFYDALKGASQGYASMSYEAIGYRP
                     AELIKLEVLINGEKEEAFAKIVPEAEAAREGRKIVSKLKEKLPPQMFALPIQAAIGGK
                     IVARETLGARRRDVTAPLYGGDVTRKNKLLKKQKEGKKKLAGRGQMRIPSEVFMSMFK
                     NN"
     assembly_gap    2189..2288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2289..3214)
                     /locus_tag="PKK37_01580"
     CDS             complement(<2289..3214)
                     /locus_tag="PKK37_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR81105.1"
                     /translation="MNGENNNGVNLEKIRNFVIISHVDHGKSTLADRFLELTGTVKKE
                     KLQAQYLDGMDLEKERGITIKMTPVRMEYKGHILNLIDTPGHVDFGYEVSRSLEAVEG
                     AILLVDVMQGIQAQTLANLDLARKQNLKIIPAINKIDLPGAAVEDARLDLAGLLGISP
                     DDVFAISAKSGANAEALLDAVIAQVPPPRGDLKKPLRALVFDSQYDSYQGVIAYVRLF
                     DGRVAAGDKLFLIQNKVDGLAKETGCFLPQMSKTDSLECGQIGYIATGVKDPEKVRVG
                     ETVTQAWAYNEKTKTYSVGALPGYDEPKPMIFA"
     gene            complement(3211..4845)
                     /gene="murJ"
                     /locus_tag="PKK37_01585"
     CDS             complement(3211..4845)
                     /gene="murJ"
                     /locus_tag="PKK37_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNR81106.1"
                     /translation="MSFHFFGNGLLNIESKGIAAPALILAVSSVASRSLGILRDWLLA
                     QRFGAGPDLDVYYAAFRAPDFVYNLLVFGGITVAFLPLFSDYFQKSKDEAWRFAANLL
                     NVFAFFLILAGAVGFLFAPALAAAVAPGFSPAQLEQTAALMRVMFLSPVIFGAASVFS
                     GILQYFKRFLAYSLAAPLYNLGIIAGIVFLTPAMGIMGVAAGVIAGALAYLLVQIAPA
                     IKSGFVWRPIFDFKDPALARAVALILPRVAGIAANQINLIAPVVIASSLAAGSIAVFN
                     LANNIFSLPAGIIGVSYATAAFALFSKYAAEGRAADLAQKFSQTMRQVGFFTVPAAIL
                     IFLIRDPLVRFLYQRGEFTAEAASLTAAALGIFCLGIYFSSALPLMFRLFFALRDTFL
                     PTLSTIIAVAANLAMNIWFVFALRQELWDDFVRRWFDLPAGDIAVLGLPLAYGLANVL
                     QFFVLWFFLFKKNRVLGVSGEIARSLGKTALAGIFMGIVVYWTIDIIGGIAGQNNLMV
                     LALAGTAAVIAYAAAATALKIPEAAMFWRALNVKKQ"
     gene            complement(5315..6475)
                     /locus_tag="PKK37_01590"
     CDS             complement(5315..6475)
                     /locus_tag="PKK37_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNR81107.1"
                     /translation="MEETKIKNLAAAAKRIKTAIGAGENIIVYGDTDLDGVCAAIMIG
                     EMIKNAGGETAAYYFPDREKEGYGITKTALEKLARLAPALLVAVDLGIGNFEEVKLAR
                     RAGFSVIIVDHHEILGGVPEADIVIDPKQPGDDYPFKFLAASGLVFKLAEEFFGADFC
                     GNLRQSFAEMAALATIADMMPRQDDNIEIINEGLAALKRSFRPGLRAFFDTDAFFEND
                     SRNFKSRLNKIIAVLNVRDIRNGLPAAFDLLTTDSLEKSKKLIREFLEINKLRREKIN
                     VAVAEIETQIDKSAPLIFCGRRDWDLNLLGTIASILAINHNKPVFVYKILNGQVQGAA
                     RSIDGVDSVALMKKCSELLITFGGHPKASGFRAKTENLEKFKECLIKNLDKS"
     gene            complement(6456..7700)
                     /gene="pgk"
                     /locus_tag="PKK37_01595"
     CDS             complement(6456..7700)
                     /gene="pgk"
                     /locus_tag="PKK37_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNR81108.1"
                     /translation="MRTLSEGDFKNKKTLVRVDFNVALGPKGEVVDDFRIVKTLPTIK
                     FLAESGAIVVLMSHLETEDGPVSLRPVSRRLAELLGRPVKFLRDCVGEKTREEISGVG
                     AGEIILLENLRFHEGEKRNDAEFAAELAKNGDCYVNEAFSCCHRAHASIAGVPRLLPS
                     YGGMLLAEEIGSLRKIVKNPARPLVAIIGGVKMDTKAKVIAAIAAIADHVLIGSKIGA
                     EILAHKQQIAGREIFKFDSQAAEIDLTSPKIHLPIDAVMALKDASEGYSRIAGPGMMR
                     GEENIYDIGPETAKFFAEIIKDAKTIFFNGPMGWFERQEFSGGTRAVIEAISRTHGAW
                     RAAGGGQTLEAIAKFKAEENFNFLSTGGGAMLEYLAGNVLPGIAALENLPSGKNETPV
                     IPMPLPAGGLSGDKKKNGRNEN"
BASE COUNT         1935 a         2245 c         1853 g         1857 t
ORIGIN      
        1 tgagagaacg aggcaagccg caaagaaaag cgaatgatcc gaaaccgctt tgcggtttgc
       61 tccattgcgg aaattgcggg atgggaataa ccgccgaggt cagaaaaggc catacctatt
      121 atcgatgtac caaaaaatca aaaaccgtta agtgtttgga aaagtatatc cgtgaggaag
      181 agcttgatcg gcagctatcc caagaattgc aaaaattttc tttgcccgaa gattgggcaa
      241 gccgattatt gcagatgacc gtaaaagaag aacaaggact tgcccaatct tccgccgtcc
      301 tcacccaggc atcgcgcgat gagctagaag acataaaaac caagctcccg cgccttttgg
      361 acagttatct tgaccaagat atcgaccgcg atgactatcg ccaaaagaag gccgacctta
      421 tgagccggaa gaagacgctg gaagagaaaa tcggcgacct tgaacagggc cgtaatagtt
      481 ggctcggacc gatgcgggaa tggctaaatg aagcgcaaaa tataggaaaa atcgctcaaa
      541 attcggatct ttccgccaaa aaggtatccg ccaaaaaaat cttcggctcg gacctgcatc
      601 tttccgccaa aaaaatcacg ggaaggcccg caattcaatg ggcggcgata gccgccgcga
      661 gaaattttcc tcctaaaact gacttaagtt acttaatgga gcgagtgaag ggaatcgaac
      721 cctcgtctcg accttgggaa ggtcatgtac tgccactgta ctacactcgc aaaccgccta
      781 tcggcgcaat tttcaattac caaataaatt caaaattaaa acttgaagct tttggcttgc
      841 gcttttggct tttaaccttt gatttttgat atttatttga tgtttttgat ttttttccag
      901 aggtcggccc agaagctgtt ggcggcggaa acgccgcctt cttgatttgt agcagaagtc
      961 gccgggtcgc gcaagatggc aatcacttct tcgccgggtt ttttgtagcc ctgttcgcgc
     1021 aggcgttcct cctgaaaact ttccccttgc acggcttcca gtccggcttg aagcgtccgg
     1081 cgatgctcct ccaaaatctg aacttggttt tgtaaatctt tgatattggc atccagatcg
     1141 cggcggttgt tccatatccg ccaattggaa aacgctaaaa acgcgatgat cgccagcgcc
     1201 gtcagtccca tcgccagcgc cgtcaaacgg gcgccgaatg attttctctt tttgattttg
     1261 ggaattttaa ccgccacagt attgaaattt caaatttgtt tcggatttcg cattttttcc
     1321 accggcttga cgaaaacgcg aaaataaacg ataaaggaag catcggcgcc gatcattgat
     1381 tttacctcaa aacaaatcaa aaatgaaatc aaaaaaaata atgaagaccg tctggaaagc
     1441 gatggtggtg gtgatcgcct taagtatggt gctttccatg gtggccatgg gatggtaaaa
     1501 aaagccggac gcgcgtccgg cttttttagt tgtttttaaa cattgacata aagacttccg
     1561 acggaatccg catctgtccc cgcccggcca gctttttttt accttctttt tgttttttca
     1621 aaagtttgtt tttccgcgtc acgtcacctc cgtataacgg cgcggtcacg tcccgccggc
     1681 gggcgcccaa agtctcgcgc gcaacgatct tgccgccaat cgccgcctgg atgggcaagg
     1741 caaacatttg cggcggcaat ttttctttca atttggaaac aattttgcgc ccttcgcgcg
     1801 cggcctcggc ttcgggaacg attttggcaa acgcttcttc cttctcgccg ttgatcaaca
     1861 cttccagttt aatcaattcc gccggccggt agccgattgc ctcgtaactc attgacgcat
     1921 atccctggct ggcgcctttc aacgcgtcat aaaatccgct gacaatcttg cgcaagggaa
     1981 tttcataggc cagcaggagc ttttcgttgc cgacataatc ggtgttaacg taaattgccg
     2041 cgtattcctt ggccaaatcc gagatcggcc ccaaataatc aatcggcgtg acgatttcca
     2101 atttcaccca cggttcccgg gtgtccttga tcgccgtcgg atcgggccag tcgccggtgg
     2161 tatagatcgt tttttccgcg ccgcggccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnngc gaagatcatc ggcttgggtt cgtcatagcc gggcagggcg ccgaccgaat
     2341 aggtttttgt tttttcgtta taagcccagg cctgcgtaac ggtttccccc acccgcacct
     2401 tttccggatc cttgacgccg gtggcgatat agccaatctg gccgcattcc aaagaatccg
     2461 tttttgacat ttgcggcaaa aaacaaccgg tttccttggc caggccgtca actttgttct
     2521 gaatcaaaaa cagcttgtcg ccggcggcta cccgtccgtc aaacaggcgc acataggcga
     2581 tcactccctg ataggaatca tattgcgaat caaacaccag cgcgcgcaga ggctttttca
     2641 agtcgcctcg cggcggcggc acttgagcga tcaccgcgtc aagcaaagct tccgcgttgg
     2701 cgccggactt tgcggagatc gcaaaaacat cgtcggggga aatccccagc aatcccgcca
     2761 gatcaaggcg ggcgtcttcc accgccgcgc caggcaggtc aattttgttg atggccggaa
     2821 taatttttaa attttgtttg cgcgccagat ccagattggc cagagtttgc gcctggatac
     2881 cctgcatcac gtccaccaaa agaatcgcgc cctccaccgc ttccaggctc cggctcacct
     2941 cataaccgaa atccacgtgg ccgggagtgt caatcaaatt gagaatgtgc cccttgtatt
     3001 ccatccgcac cggcgtcatt ttgatggtga tgccgcgttc tttttccaag tccataccat
     3061 ccagatattg ggcttgcagt ttttcttttt tgaccgtgcc ggtcaactcc aaaaaccggt
     3121 cggccaaagt tgatttgccg tgatccacat gggaaataat cacaaaattg cggatctttt
     3181 ccaaattcac gccattattg ttttctccgt tcattgtttt ttcacattca aagcccgcca
     3241 aaacattgcc gcttccggaa ttttcagcgc cgtcgcagcc gccgcgtaag cgatcaccgc
     3301 cgccgttccc gccagcgcca aaaccatcaa attattttgt ccggcaattc cgccgataat
     3361 atcaatcgtc caatatacca caattcccat aaaaattccc gccagcgcag tcttgcccaa
     3421 agaccgcgcg atttctccgc tcacgcccag cacccggttc tttttgaaaa gaaaaaacca
     3481 aagaacgaaa aactgcaaca cgttggccag cccgtatgcc agcggcaacc ccaaaaccgc
     3541 gatgtcgccg gccggcagat caaaccatcg gcggacaaaa tcgtcccata attcctggcg
     3601 caaggcaaaa acaaaccaaa tgttcatcgc caaattggcc gccaccgcga taatcgtgga
     3661 caatgttggc aaaaatgtgt cgcgcagggc aaaaaacaag cggaacatca gcggcagagc
     3721 ggacgaaaaa taaattccca aacaaaaaat tcccagcgcg gcggcggtga gtgacgcggc
     3781 ctcggcggtg aattcgccgc gctggtataa aaagcgcacc agcggatcgc gaatcaaaaa
     3841 aatcaagatt gccgccggca cggtgaaaaa tccgacctgg cgcatcgttt gggaaaattt
     3901 ttgcgccaaa tccgcggctc tgccctcggc ggcgtatttg gaaaacaaag caaacgccgc
     3961 cgtggcatag gaaacgccga tgatgcctgc cggcagggaa aagatgttgt tggcgagatt
     4021 gaacaccgcg attgatccgg cggccagcga tgaagcgatc accacgggcg cgattaaatt
     4081 aatctgattg gccgcaatgc ccgcgacgcg cggcaatatc agcgccaccg cccgcgccag
     4141 cgccggatct ttgaaatcaa aaatcgggcg ccacacaaat cccgatttga ttgccggcgc
     4201 gatttgcacc agcagataag ccagcgcgcc ggcaatcacg cccgcggcaa cgcccataat
     4261 tcccattgcc ggcgtcaaaa aaactatgcc cgcgataatc cccaaattgt aaagcggggc
     4321 ggccaggcta taagccagaa atcttttgaa atattgcaaa attcccgaga acaccgatgc
     4381 cgcgccgaaa atgaccggac tcaaaaacat cacgcgcatc agcgcggcgg tctgttccag
     4441 ctgcgccggc gaaaatcccg gcgccacggc cgccgccaat gccggcgcaa aaagaaaacc
     4501 gacggccccg gccaaaatca aaaaaaacgc gaaaacgtta agaaggttgg cggcaaaacg
     4561 ccacgcttca tctttgcttt tttgaaaata atccgaaaac agcggcaaaa acgccaccgt
     4621 gatcccgccg aaaaccagca gattataaac aaaatccggc gcgcgaaacg ccgcgtagta
     4681 aacatccaaa tccggacccg cgccgaagcg ttgggccaaa agccagtcgc gcaaaatccc
     4741 caacgaccgg ctggcgacgg aagataccgc caaaatcagc gccggcgccg caatcccctt
     4801 gctctcaatg tttaacaacc cgttgccaaa aaaatgaaaa gacatagtat ttttccaagg
     4861 tctaaccttc gcgattgcgc gaaggttaga ccttggaaaa cgaaagaccc aagccggcaa
     4921 taagccgaat tctgtctaat cccgccggat ttggcgaaac gagacgatta tttatctgga
     4981 ccgcaaatta ctctgcggcc tcaagcggtt cacttttcac cccgccaaag gcggtatgtt
     5041 taaccttgca tccggtaagg atttagccgt ttcaccccta tgttgccaca ggattgatcc
     5101 cttgcctttc ggttcgggac cctctcgcct ttcggtttga gcgtcactgt tcgcacctcg
     5161 ggcctcgcgg ccgaagggtg ttgccctcta cctttttaga taccttgcgg caaatggaag
     5221 ttcggacttt cctccgggca tattgcaaac aatgcgcccg gcaatcgtct tgccggcttg
     5281 ggtcttccgt ccggccttat tttaacaaac aaaatcacga tttgtcaagg ttttttatca
     5341 gacactcttt gaatttttcc agattttcgg ttttggcgcg aaagcccgac gccttgggat
     5401 gtccaccgaa ggttatcagc aattccgagc attttttcat cagcgccacg ctgtcaacgc
     5461 cgtcaatgct tcgcgccgcc ccctgcacct ggccgtttaa aattttataa acaaagaccg
     5521 gtttgttgtg gtttatcgcc agaatggaag caatggtccc cagtaaattg agatcccagt
     5581 cgcggcgacc gcaaaaaatc aacggcgcgc ttttgtcaat ttgcgtttca atttcggcga
     5641 cggcgacgtt gattttttcc cgccgcaatt tgttgatttc caaaaattcg cggatcaatt
     5701 ttttggattt ttccagcgag tcggtggtga gcaagtcaaa cgccgccggc aagccgttgc
     5761 gaatgtcgcg gacattgagc acggcaataa ttttgttgag acggcttttg aaattccggg
     5821 aatcattttc aaaaaacgca tcagtgtcaa aaaacgcgcg cagtccgggg cggaaagatc
     5881 ttttcagcgc cgccagtcct tcgttgatga tttcaatatt gtcatcctgc cgcggcatca
     5941 tatcggcaat tgtcgccagg gccgccattt cggcgaaact ctgtcgcaaa tttccgcaaa
     6001 aatccgcacc gaaaaattct tcggccaact tgaaaaccag ccccgacgcg gctaaaaatt
     6061 taaacgggta atcgtcgccc ggttgtttgg gatcaatcac gatgtcggcc tcgggaacgc
     6121 cgcccaaaat ttcatgatgg tcaacgatga tcacggaaaa tcccgcccgg cgcgccaatt
     6181 ttacttcttc aaaattgccg attcccagat caacagccac caaaagcgcc ggcgccaatc
     6241 gcgccagctt ttccagcgca gttttggtga tgccataacc ttctttctcg cggtcgggga
     6301 aataataagc ggcggtttcg ccgccggcat tcttgatcat ttctccgatc attatcgccg
     6361 cgcaaacgcc gtccagatcg gtgtcgccgt aaacgatgat gttttcaccg gcgccaatcg
     6421 cggtttttat tctttttgcc gccgcggcta aatttttaat tttcgtttct tccatttttt
     6481 ttcttgtcgc cggacaaacc gccggcgggt aaaggcatcg ggatcaccgg cgtttcgttt
     6541 ttgccggaag gcaaattctc aagcgccgcg attcccggca aaacattgcc ggccagatat
     6601 tccagcatcg cgccaccgcc cgtggaaaga aaattgaagt tttcttccgc cttgaatttg
     6661 gcgattgcct ccaaagtttg gccaccgccc gccgcccgcc acgcgccgtg agttcgcgag
     6721 atcgcttcaa tcaccgcgcg ggtgccgccg gaaaattcct gcctttcaaa ccatcccatc
     6781 gggccgttga aaaaaatcgt tttggcatct ttgatgattt cggcaaaaaa tttcgcggtt
     6841 tccggcccga tgtcgtaaat gttttcttcg ccgcgcatca ttccgggccc ggcaatccgg
     6901 gaatatcctt cgctggcgtc tttgagcgcc atcaccgcat caatcggcaa atgaattttg
     6961 ggcgatgtga gatcaatctc cgcggcctgg gaatcaaatt tgaaaatctc cctgcccgcg
     7021 atctgttgct tgtgcgccaa aatttcggcg ccaatctttg atccgatcaa gacatggtcg
     7081 gcgatggcgg cgatggcggc gataaccttc gccttggtat ccattttcac gccgccgatg
     7141 atcgccacca gcggccgcgc gggattcttg acaatttttc gcagactgcc aatttcttcg
     7201 gccaaaagca tccccccata cgacggcaaa agccgcggca cgcccgcgat tgacgcgtgc
     7261 gcccggtgac agcaggaaaa cgcttcgttg acatagcaat ccccgttttt ggcaagttcg
     7321 gcggcaaatt ccgcgtcgtt tcgcttttct ccctcgtgga aacgcagatt ttccagcaaa
     7381 atgatttccc ccgcgccgac gccggaaatt tcttcgcgcg ttttttcgcc gacgcaatcc
     7441 cgcaaaaatt tcaccggccg gcccaataat tccgccaaac ggcgcgatac cggccgcaga
     7501 ctcaccggac cgtcctcggt ttccaagtgg ctcatcagca ccacgattgc gccgctttcc
     7561 gccaagaatt ttatcgtcgg caaggttttg acgatgcgga aatcgtcaac cacctccccc
     7621 ttcggcccga gagcgacatt gaagtcaacc cgcaccagcg tttttttgtt tttgaaatcg
     7681 ccttcgctta atgttctcat ttttttgctt taatcggcgc gcggcgcgcg ccattctctc
     7741 atttccccat tctaacacgc cccaccattt gcgccaaccg cgccggcaac cgtattcgtt
     7801 cgtgtcatta ctcgtgtaat tacatatgtc atcatctgtt gaaaaattca aagggtctaa
     7861 ccctctgaaa atcctccgca aaactaccgc accggattgg gatcgctcac cccgccctct
     7921 tcgcagctgt aaacgacaac atccctgccg gcaccggtta cccaaagcca ataacgaccg
     7981 gcgtctaaat
//
LOCUS       DAOQWE010000025         7471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_10281_length_7471_cov_7.377967, whole genome shotgun sequence.
ACCESSION   DAOQWE010000025 DAOQWE010000000
VERSION     DAOQWE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10281_length_7471_cov_7.377967"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PKK37_01600"
     CDS             complement(<1..281)
                     /locus_tag="PKK37_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNR81109.1"
                     /translation="MMKDSYIDYAMSVIVSRALPDVRDGLKPVQRRVLYVMLEEGLRH
                     TAKYRKSATVVGSALGRYHPHGDSSVYDTMVRLAQDFTLRYPLVQGQGN"
     gene            complement(460..828)
                     /locus_tag="PKK37_01605"
     CDS             complement(460..828)
                     /locus_tag="PKK37_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR81110.1"
                     /translation="MSEFLNGNCKYDLEDRTLKFSENIIIAIKNFPKNIASTPIIKQL
                     IRSATSIGANYREANGAASKKDFINRIHFCKKEAKETMYWLQLLAKAIEDEKSKNVCR
                     VLWKESQELTFIFSKIVSKK"
     gene            complement(868..1554)
                     /locus_tag="PKK37_01610"
     CDS             complement(868..1554)
                     /locus_tag="PKK37_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR81111.1"
                     /translation="MQIIWHGQSCFEIIIPKGKGENITLLVDPPSEAKTGLKPPARTG
                     AQIVLLTNASYTAGKEDLEKIAPGAFLIDGPGEYESRGIYIRGIDFNFGKAGVEQDAA
                     APKSFAMGTIYTIEAEEMKLCHLGNFSDKELAAAQVEKIGNVDILMFPAGDGKNFGAK
                     EAMRVVSQIEPSIAVPMNYEIPKLKLEAGDLKEFLKTAGISQTEPLAKLTIKKRDINL
                     EEAKIIVLNP"
     gene            complement(1624..2097)
                     /locus_tag="PKK37_01615"
     CDS             complement(1624..2097)
                     /locus_tag="PKK37_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81112.1"
                     /translation="MIREFIENFFHQAPGLMFELASAAIILLFGGYFAQTIGAAADDF
                     LAHAKIARAAGKSKLLKAAIKKKIAGKALKFWILIVFAMMSAKIMGLEVVAGFLGKIA
                     LAGVCFFAAVFVYFAAVIATNLWRSKSFRAKAIVGKFAGRIRKMAGRFKKKATGV"
     gene            complement(2108..2680)
                     /locus_tag="PKK37_01620"
     CDS             complement(2108..2680)
                     /locus_tag="PKK37_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HNR81113.1"
                     /translation="MEVDIIFFVSCIYFFLPAYFANMTPPLVRNAKVFDFLAKPVDLG
                     KSWRGKRIFGGHKTWRGVVCAIAVGMGTALLQWWLYQFPWATELSLLDYKSVNLWVFS
                     LLFAGGAVAGDLAAAFVKRRLNLPPGAPFMPWDQTNYVIGSFIFLGAYVGKYLTGDLL
                     VWGAIFVMTFFLHLLFNRFGYDIGLHKAKW"
     gene            2708..3418
                     /locus_tag="PKK37_01625"
     CDS             2708..3418
                     /locus_tag="PKK37_01625"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNR81114.1"
                     /translation="MIEFQIAINKPMIKCDLHIHSKYSFDSLADPEKIADLALARGIQ
                     CIAVADHGNIKGSLEAAEYAKKKNLPILVIISEEVKSKQGDILALNIKEPIPDHLPAG
                     ETLKRIKDQNGLAIIAHPFGSFCGFKENLSNYFGQFDGIEILNGSVFSGNEEAEDFAQ
                     KHNLPFTAGSDAHFANRFVGTVWLELPLDWLPNLTPGQIIAAIKQKKGAAVGTPEPFI
                     SKALDHCFRSLTKLKALV"
     gene            complement(3446..3760)
                     /locus_tag="PKK37_01630"
     CDS             complement(3446..3760)
                     /locus_tag="PKK37_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81115.1"
                     /translation="MTVCQFYVKFQTKEDCKRDGIKMAIKTGDRVKIPEESPLEVSGG
                     CSLATIWGSVEIVRIEAPWAIIKPFQCFMITGLAGGKESKITGGEFQISLEEINKIGR
                     VF"
     gene            complement(3863..4279)
                     /locus_tag="PKK37_01635"
     CDS             complement(3863..4279)
                     /locus_tag="PKK37_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNR81116.1"
                     /translation="MPNTLPTGTLGERLAADYLKSRGYRILGRNFFLRALGGPKIAEV
                     DIIAEKSGIFVFAEVKTIKANSKYLAQDKVNETKKWKIAKAAEMWLIKNKVPLDVKWQ
                     IDVIAVELLPDDRPKIFQKILGPRFKLSHFENIAIR"
     gene            complement(4230..>4897)
                     /locus_tag="PKK37_01640"
     CDS             complement(4230..>4897)
                     /locus_tag="PKK37_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81117.1"
                     /translation="VLAAPVGAHLPRMEGANAEIKIERPEISQAFYGWLEGKPAVYSI
                     NAKTPFLLYVGLLSPRVEFAQTDFSAVIRKDGKLFSRISAEDSLWPVTYEPFANDYYA
                     KGPEWEQRVPAGKYEIEVSNSGNSGSYSLAIGKTEDLSLGGFLRTLAVLPHVKEKFFG
                     VPWWQAYNNLIGVAALIFLAVLLIAAYFATSFFKNRRLKNKLDEQYAKHFADRDAGGA
                     AGG"
     assembly_gap    4898..4997
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4998..5499
                     /locus_tag="PKK37_01645"
     CDS             <4998..5499
                     /locus_tag="PKK37_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81118.1"
                     /translation="SPPAQSFFTAATFQNLRLRYNVAVGLRINDACDMNTTKKLATAA
                     ILLAAASAFLIMELPKIKIAETNNGNPAAQMILYYGNSCPHCKTVENYIAENNLAEKL
                     RIEQKEISSDRTNQDEFIRTAKSCGLGSDNLGVPMLWDAKTQKCHSGDQTIIDFLKQA
                     DTKPPL"
     gene            5496..6008
                     /locus_tag="PKK37_01650"
     CDS             5496..6008
                     /locus_tag="PKK37_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81119.1"
                     /translation="MKNKITFLIFIFALEAAAGKAEAVCPVCTVAVGAGVGLSRWLGI
                     SDLITGLWLGAFLLSLALWAVDWLGKRKIRSPFAKFAVILAGYAMAIVPLYYAEITAH
                     PIDFLRSATQDKLILGIIEGSTVFFFAVKLYEFLKERNHRRAHFPFEKIAIPIALLAF
                     FSLILHFLTI"
     gene            6013..6549
                     /locus_tag="PKK37_01655"
     CDS             6013..6549
                     /locus_tag="PKK37_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81120.1"
                     /translation="MKTPNGQIKDIDEFVAKIDEMKKKDKMDLSRDQDLAIAIMNLVS
                     IEEHFFFTAEKTGDRKYYDLLSETREMRKELLKKIVGEPEGEVWCISKHLLAASYRLM
                     EVGTKELASNKNDAYDFFEKSFKLYTLFWGLNMGAIDAAGIKKIGDGAIGKNDKKPQG
                     FVGKLGDLVKRAINCCIE"
     gene            complement(6550..7305)
                     /locus_tag="PKK37_01660"
     CDS             complement(6550..7305)
                     /locus_tag="PKK37_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81121.1"
                     /translation="MVQELGKNKNPGAGKKIRSRAYPALDLPAAILTAKKIFDDLGQG
                     PHSRMLLARGLGYGSFCGAASAKIGALAHFGLLERFAGNYSIAPAAPPLFDYPKNPRQ
                     KEIFAAAARPALFAALAARFAGQNLPKNLPAVLVSDYGISFSAAPLAAKNFIATFEFA
                     GMLKENRLILPDGEESENDGDNDIAKEMNFEEKNRRPAAGNNHGGGKEEEGIEVRLAS
                     GIRIVFPKKFAARLARGEFAEEVKSLDSKITAD"
BASE COUNT         1978 a         1894 c         1567 g         1932 t
ORIGIN      
        1 aaattgcctt ggccctgcac caaagggtag cgcaaggtaa aatcctgcgc caagcgcacc
       61 atcgtgtcat agactgaaga atcgccgtgc ggatgatagc gccccagcgc ggaacccacc
      121 accgtggcag atttgcggta tttggcggtg tggcgcaacc cttcttccag catcacatac
      181 agaacgcggc gctgaacggg cttcaaaccg tcgcggacat cgggaagcgc gcgcgacacg
      241 atcaccgaca tcgcgtagtc aatgtaggaa tccttcatca ttgacacgat ctcggcgggc
      301 ttcacctgct caaattccaa tttttcctgg ccgttgccct tgtttttgct gtctttagcc
      361 ggcttcttgt ttgccataat tcattcttac ttaataattt ttaatttgaa atttttaatt
      421 tttaaacaat ttttaattaa aaaattttaa aaaccgtttt cattttttgg aaacaatctt
      481 tgaaaaaata aatgttaact cttgtgattc tttccaaaga acacgacaca catttttact
      541 tttttcgtcc tcaatcgctt ttgctaataa ttgtagccaa tacatggtct cttttgcctc
      601 cttcttgcaa aaatgaattc tgtttataaa atccttcttg ctggccgctc catttgcctc
      661 tcgataattg gcgccaatgc tggttgccga tcggattaac tgtttaataa tcggcgtgct
      721 ggcgatattc ttcggaaaat ttttgatagc aataataata ttctcgctga atttcaaagt
      781 tctatcctcc aagtcatact tgcaatttcc gtttaaaaat tcgctcattt ttaaattgtt
      841 taaaaattaa aaatttcaaa ttaaaaatta aggattcagc acgatgattt tggcttcttc
      901 aagattaatg tccctttttt ttatcgtgag ttttgccagc ggctcggttt gggaaatgcc
      961 ggcggtcttt aaaaattcct tcaaatctcc ggcttccagt ttcaatttgg gaatctcata
     1021 attcatcggc accgcaatgc tgggctctat ctgcgacacg accctcatcg cctccttggc
     1081 gccgaaattt ttcccgtcgc cggcgggaaa catcaaaatg tccacattgc cgattttttc
     1141 cacctgcgcc gcggccaact ctttgtcgga aaaattgccc aaatggcaaa gtttcatctc
     1201 ttcggcttca atggtataga tcgttcccat ggcaaaggat ttcggcgcgg cggcgtcttg
     1261 ctcaacgcca gccttgccga aattgaaatc aattccccga atgtagatgc cccgggattc
     1321 gtattctccc ggaccgtcaa tcaaaaacgc gccgggagcg atcttttcca aatcctcctt
     1381 gccggcggta taggacgcgt tggtcagcaa cacgatctgc gcgccggttc ttgccggcgg
     1441 cttgaggccg gttttggcct cgctgggcgg atcaacgagc agggtgatgt tttccccctt
     1501 acccttggga atgatgatct caaaacaaga ttgtccgtgc caaattattt gcataaaatc
     1561 atttcaaaaa acaaaattgg aatggttcca attcgttcat tctaccacag cggcaagata
     1621 aattcaaact ccggtagcct tctttttgaa acggccggcc atttttctaa ttctgccggc
     1681 gaatttgccg acgatggctt tggcacggaa agattttgac cgccaaagat ttgttgcgat
     1741 aactgccgca aaataaacga aaaccgcggc aaaaaagcaa actccggcca gtgcgatttt
     1801 tcccagaaat ccggcaacaa cctccaaccc catgattttg gcgctcatca tcgcaaaaac
     1861 gatcaatatc caaaatttca acgccttacc cgcgattttc ttcttaatgg cggccttcag
     1921 cagttttgat ttgccggcgg cgcgcgcaat tttggcatgc gccaaaaaat catccgccgc
     1981 cgcgccgatg gtttgagcga aatagccgcc gaaaagaaga atgatcgccg ccgaggccag
     2041 ttcaaacatc aatcccggcg cctggtggaa aaaattttca ataaattccc gtatcatcgc
     2101 aattttgtca ccatttggct ttgtgcaagc cgatgtcata accgaaccga ttgaacagca
     2161 gatgcaaaaa gaaagtcatc acaaaaatcg ccccccacac cagcagatcg ccggtcaaat
     2221 atttgcccac gtacgccccc agaaaaatga aactgccgat gacataattg gtttggtccc
     2281 aaggcataaa gggcgcgccg ggaggcaggt tgagccgccg tttgacaaac gccgcggcca
     2341 aatcccccgc caccgcgccg ccggcgaaca acagcgaaaa cacccaaaga ttaaccgatt
     2401 tataatccaa aagagaaagt tcggtggccc agggaaactg ataaagccac cactgcaaaa
     2461 gcgccgtccc cattcccacg gcaatggcgc acaccacccc ccgccaggtt ttgtggccgc
     2521 cgaaaatccg cttcccgcgc cagcttttac ccaaatcaac cggctttgcc aaaaaatcaa
     2581 acactttggc gtttctaacc aaaggcggcg tcatattggc aaaatacgcc ggcaaaaaga
     2641 aatatatgca agatacgaaa aaaattatat caacctccat gttcgctctg ttattatccg
     2701 tgttattatg atagaatttc aaattgcaat caacaaaccg atgatcaaat gcgacctgca
     2761 catccacagc aaatattcgt ttgacagctt ggccgatccg gaaaaaatcg ctgacttagc
     2821 attggcgcgc ggcatccaat gcattgccgt cgccgatcac ggcaatatca agggatcgct
     2881 tgaagccgcc gaatacgcca aaaaaaagaa cctgccgatt cttgtcatta tatcagaaga
     2941 agtgaaatca aaacagggcg acattctggc gttgaacata aaagaaccaa tccctgacca
     3001 tttacctgcc ggcgaaacat taaaaaggat caaagaccaa aacggactgg cgatcatcgc
     3061 ccacccgttc ggttcgtttt gcggcttcaa ggaaaatctg tcaaattatt tcgggcaatt
     3121 tgacggcatt gaaattctca acggctcggt attttccggc aatgaagaag ccgaagattt
     3181 cgcacaaaag cataacttgc cgttcaccgc cggatccgat gcccactttg ccaaccgctt
     3241 cgtcggcacc gtttggctgg aattgccgct tgactggttg ccgaatttga cgcccggcca
     3301 aattattgcc gccatcaaac aaaaaaaggg agccgccgtc ggcactcccg aaccttttat
     3361 ctcaaaggct cttgatcact gtttccgctc tttaaccaaa ttgaaagcat tggtttaaaa
     3421 aaagaggaga caaaatttct ccaagttaaa atactctccc aatcttattg atttcttcca
     3481 gagaaatttg gaattctcct ccagtgattt ttgattcttt acctcctgca agccccgtga
     3541 tcataaaaca ttggaacggt tttatgatcg cccacggagc ctctattctt acaatttcca
     3601 cacttcccca tatcgttgcc aaggaacaac ccccgctcac ttccagcggt gattcttcgg
     3661 gaatttttac cctatcgccg gtttttattg ccatcttgat accatcccgt ttgcagtctt
     3721 ctttagtctg aaattttaca taaaattggc acactgtcaa attttttttc actaatttgg
     3781 caaaattcaa agccaaaagt cagcgcaaac tcgccaattt taaaatttat ggaatatact
     3841 ccattctttt caacttttaa atttaacgaa tggcaatgtt ttcaaagtgg gaaagtttga
     3901 agcgggggcc gaggattttt tggaagattt ttgggcggtc atcgggcaag agttccacgg
     3961 cgatcacgtc aatttgccat ttgacgtcaa gcggcacctt gttttttatc agccacattt
     4021 ccgcggcttt ggcaatcttc catttttttg tttcgtttac cttatcctgc gccaaatatt
     4081 tggaattggc cttgatggtc ttcacctccg caaaaacaaa aataccgctc ttttcggcaa
     4141 taatgtccac ttcggcgatt tttggcccgc ccaaagcgcg caagaaaaaa ttccgcccta
     4201 aaattcggta gccccggctt tttaaataat cagccgccag ccgctccccc agcgtcccgg
     4261 tcggcaaagt gtttggcata ttgttcgtcc aatttatttt tcaagcgccg gtttttgaaa
     4321 aacgacgtcg cgaaatacgc cgcgatcagc aacaccgcca ggaaaatgag cgccgccacg
     4381 ccgatgagat tgttataggc ctgccaccag ggcacgccga aaaatttttc cttgacgtgc
     4441 ggcagaaccg ccagcgtgcg caaaaaaccg cccaaagaca gatcttcggt tttgccgatc
     4501 gccagagaat aactgccgct gttgccggaa ttggaaactt caatttcata ttttccggca
     4561 ggaactcttt gctcccattc cgggcccttg gcataatagt cgttggcaaa cggctcatag
     4621 gtgaccggcc acaaagaatc ctctgccgaa atacgggaaa acaatttgcc gtctttgcgg
     4681 attaccgccg aaaaatccgt ctgcgcgaat tcaacccgcg gcgacaacaa cccgacataa
     4741 agcaaaaacg gcgttttggc attgattgaa taaaccgcgg gcttcccttc cagccagcca
     4801 tagaacgcct gggaaatttc cggcctttca attttaattt cggcgttcgc cccttccatc
     4861 cgcggcagat gcgcgccgac cggcgccgcc aaaacgannn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnttc tccgccggcg caatcttttt tcactgccgc aacttttcaa
     5041 aatttacgct tgcgctacaa tgtagctgtc ggactccgga ttaatgatgc ttgcgatatg
     5101 aacacaacca aaaaactggc aactgccgca attttgttgg cagcggcctc ggcgttttta
     5161 ataatggaat tgccaaaaat aaaaattgcg gaaacgaaca acggcaaccc cgccgcgcaa
     5221 atgattctgt attacggaaa ttcctgtcct cattgcaaaa ccgttgaaaa ttacatcgcc
     5281 gaaaataatt tggccgaaaa attgcgaatt gaacaaaaag aaatttcaag cgaccggaca
     5341 aatcaagacg aattcatccg gaccgccaaa agttgcggcc ttggttcgga caatttgggc
     5401 gtgccgatgc tctgggatgc caaaacgcaa aaatgccatt cgggcgacca aaccatcatt
     5461 gattttctga aacaagcgga tacgaaaccg ccattatgaa aaacaaaatc acgtttctta
     5521 tttttatttt tgccttggaa gcggcggcgg gaaaagccga agcggtttgc ccggtgtgca
     5581 ccgtggccgt gggcgccggc gtcggtcttt cgcgctggct gggcatcagc gacttgatca
     5641 ccggattatg gctgggcgcg tttttgcttt cgctggctct gtgggccgtt gactggctgg
     5701 gcaaaagaaa aatccgttcg ccgttcgcca aattcgccgt gattttggcc ggctacgcca
     5761 tggcaatcgt gccgctttat tacgccgaaa tcaccgccca tccaattgat tttctccgct
     5821 ccgccaccca ggacaaattg attttgggaa ttattgaagg ctcgaccgtg tttttctttg
     5881 ccgtaaagtt gtatgaattt ttgaaagaac gcaaccacag acgcgcccat tttccctttg
     5941 aaaaaatcgc catacccatc gcgcttcttg cttttttttc gttgatactc cattttttaa
     6001 caatttaaaa atatgaaaac acccaacgga cagataaaag atattgatga attcgtcgcc
     6061 aaaattgatg aaatgaaaaa gaaagacaaa atggatcttt cccgcgatca ggatctggcc
     6121 atcgcgatca tgaacttggt gagtattgag gaacatttct ttttcaccgc cgaaaaaacc
     6181 ggcgacagaa agtattatga tttgctttcc gaaacgcgcg aaatgcgcaa agaattgctc
     6241 aaaaaaatcg ttggtgagcc cgaaggcgaa gtttggtgca tttcaaaaca tctgctggcg
     6301 gcgtcttacc gcttaatgga agtgggcacc aaggaattgg cgtcaaacaa aaacgatgcc
     6361 tatgattttt ttgaaaaatc tttcaagctc tacactttgt tctggggatt gaatatgggc
     6421 gccattgacg cggccggaat aaaaaaaatc ggcgatggcg ccattggcaa aaacgacaaa
     6481 aaaccgcaag gattcgtggg aaagctcggc gacttggtca aacgggcaat caactgttgc
     6541 attgagtgat tagtctgccg tgatttttga atccaaactc tttacctctt cggcaaattc
     6601 gccccgggcc agccgcgcgg cgaatttttt gggaaaaacg atcctgatgc ccgacgccag
     6661 ccgcacttct attccctctt cttctttccc gccgccgtga ttgttccctg ccgccggacg
     6721 gcggtttttt tcttcaaaat tcatttcttt tgcaatatcg ttgtcgccat cgttttcgct
     6781 ctcttcgcca tcgggcaaaa tcagccgatt ctcttttagc attcccgcaa attcaaatgt
     6841 cgcgataaaa ttttttgccg ccagcggtgc ggcagaaaaa gaaatgccat aatcggaaac
     6901 caagactgcc ggcaaatttt tgggtaaatt ttgccccgca aaacgcgcgg ccagcgcggc
     6961 aaacagcgcg ggccttgccg ccgccgcgaa aatttctttt tgccggggat tttttggata
     7021 atcaaaaagc ggcggggcgg cgggagcaat ggaataattt ccggcaaacc gctccaggag
     7081 cccaaagtga gccagcgcac cgatctttgc cgatgccgcg ccgcaaaacg aaccataacc
     7141 caatccccgt gccagcaaca tccgagaatg cggtccttgc cccaaatcgt caaaaatttt
     7201 ttttgccgtt aagatcgcgg cgggcaaatc cagcgcggga taggcgcggc tgcggatttt
     7261 tttgcccgcg ccgggatttt tgtttttccc caattcttgc accatttctt tatttttatc
     7321 tttttttatc ttaacaaatc aaaaatggat gggcaaataa agagtttagg cttaaaaaat
     7381 agcttaatcc acagccaagc ggagttttcc acaggttgcg agataaattc attattttta
     7441 tctcaaatat gagataaaaa tatatatgcg c
//
LOCUS       DAOQWE010000026         7346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_10506_length_7346_cov_6.893156, whole genome shotgun sequence.
ACCESSION   DAOQWE010000026 DAOQWE010000000
VERSION     DAOQWE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10506_length_7346_cov_6.893156"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1912
                     /gene="dnaK"
                     /locus_tag="PKK37_01665"
     CDS             2..1912
                     /gene="dnaK"
                     /locus_tag="PKK37_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNR81122.1"
                     /translation="MARTIGIDLGTTMSEMAAIINGEPKILENKEGSRLTPSVVALTK
                     TGERLVGVLAKRQQITNPKNTLFSVKRLIGRRFADQEVQKDLKLLPYEMRERAQDGGV
                     EVKMGDKWYAPAEISAMVLQKIKQDAESKLGESVTGAIITVPAYFDDSQRKATKLAGE
                     IAGFEVKRIINEPTAAALAYGLTKKKNEQVLVYDFGGGTFDVSILDISEDTVEVKATG
                     GDNHLGGDDVDQRIMDWLVAEYQKDNGVDLSKDELALQRLKEAAEKAKIELSTAMETE
                     INLPFITSDASGPKHLYYKLSRAKLEDLVRDLIERSVNLVRQTLGDAKMAPQDIEEIV
                     LVGGQTRMPIIQEEVKKFFGKEPNKSINPDEVVAIGAAVEAGILQGEVKDVLLLDVTP
                     LSLGIETMGGVNTILISKNTTVPTAKSQVFSTAADNQTSVEIHVLQGERPMSADNKSL
                     GKFILDGIMPAPRGIPQVEVSFDIDANGILSVTAKDKATGKTQSVRIEGSAGISKEEA
                     ERMKQEAQANAESDRKKQELITARNNADNLIYTAQKAMRDAGDKVSADAKKEIEEKIE
                     GLKKVQSGDNMEELKAKSDDLSQTLSKIGAQMYQQQSGAGTQSAGNEAAGSAGDGKKK
                     DEGAAEGEYQEK"
     gene            2042..3136
                     /gene="dnaJ"
                     /locus_tag="PKK37_01670"
     CDS             2042..3136
                     /gene="dnaJ"
                     /locus_tag="PKK37_01670"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNR81123.1"
                     /translation="MSKDYYKILGVQKEATADEIKKAYRALAHKYHPDKGGSAEKFKE
                     VNEAYQVLSDKQKRQQYDQFGNAFDGKGFSGFGGAQAGQDGWFWGRPGAEFDVQFEDL
                     GDIMQEMFGFGQPRRRRDGKRGKDIRVDAEITLEEAFTGAQKEFNISKNVACPRCSGS
                     GAEPGTKIKECFSCRGTGQVQQIKKTFLGSFTKYTVCPECRGEGYRPEKPCNVCQGEG
                     RVKTEETIKVDIPAGVDNNQVIKLEQRGEAGRKGGPAGDFYVRVFLKAHPVFERKGDD
                     LYILREIPLSLAALGGEVGAPTIEGKDILLKIPSGTESGKIFRVSGKGITRFNGFGRG
                     NMYVQVEIAVPKKLNKKQKEILEHLRKEGL"
     gene            3489..5738
                     /locus_tag="PKK37_01675"
     CDS             3489..5738
                     /locus_tag="PKK37_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81124.1"
                     /translation="MKNTHFIFKTIFVASLWLMFGFLNVAGAANYECGAAGEVQGCRV
                     CRQTLGSGGNLAHHWADDTTRCAVGQACVAGACVDGQPLLPPVYDVTVVKSQDTAIVK
                     DSGGNIIFSGNDYTQILRDAIAATPDHGSLYIGAGVYDGLRADQNIPINNALSGSQAH
                     YYTALPLRGKNIHIYGAGMGQTVFKLGDNQYYSGHQALIFFASNGLGLGYTGLTLANM
                     TLDGNKDKQAPFWYDGAGLILTGGERTGGKFFNLELQNSPNTGLYLGNNGSGWEAYAY
                     LDNIYSHDNRQAGVVFDNGEKIRASRLKFVNDGTVASGTVALQIINVGDHKLDLAIDG
                     AEINNGILKIGNEEYPGAGGITISNLKIDTDISNLGLIGAIYIKNNHDSPYSLADRGE
                     IKIGISSITTDTASKNHAVWIEQEPIPVVLSGGLIDAGVDVYANRAKSVSLEGLRLKS
                     SFNSLIAQDSKVYCNNCIFEPAKEQYMYSIAGAGAEIHFNNSTAVSLEKIDVSAGKVY
                     GEITLNTGACNDECSPSGAKRCAAGGVQICGSSGGCLKWGTEAVCPSGQTCRDGGCVV
                     AACTPETCASSGYDCGAVSDGCGGNLDCGGCGGGKTCSDGKCVSGCVPQTVRKCGGDG
                     NLYWYDSCGVQGELAQSCGSDELTSNYRCSGNRVQRQILKRGCLADACFTAPEWQDAF
                     DCSAGEACRDGDCVASETDSSSVQSADGGVAKIFSVTYEKYRVWIVAKIEGMRLRFIE
                     ITRQFFDKF"
     gene            complement(5816..5889)
                     /locus_tag="PKK37_01680"
     tRNA            complement(5816..5889)
                     /locus_tag="PKK37_01680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5855..5857),aa:Lys,seq:ttt)
     gene            6257..6802
                     /locus_tag="PKK37_01685"
     CDS             6257..6802
                     /locus_tag="PKK37_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNR81125.1"
                     /translation="MKRKNIFKLIASIVICQAAALIGSMFTMPAVGGWYAELNKPSWT
                     PSGGVIGAVWTVIYLLMGVSLYLAWAKNFQISENLSGMAARAWNCISQKLLDGAWQKQ
                     NIIAVFSVQLILNVLWSFVFFELKMPGWAFFELLMLWVAVAYTIANFRRVSKPAAYLL
                     LPYIAWVTFAGYLNFVIWQMN"
     gene            6857..>7346
                     /locus_tag="PKK37_01690"
     CDS             6857..>7346
                     /locus_tag="PKK37_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81126.1"
                     /translation="MANTIKKILSLILPLVIAAAFAPLVVLAQNPVNVYFFWGQGCPH
                     CEREKEFLADLAANRPEILIKDFEVWGNAENRRILGELGKELNVDVSGVPFTVIGDKY
                     FIGWYDRASTGAAIEAAVATAVEQGCRDVAAPIIDPEETAAPNNGSCGNNGKTEIPEK
                     INA"
BASE COUNT         1927 a         1610 c         2096 g         1713 t
ORIGIN      
        1 tatggcaaga acaatcggaa ttgatctggg aaccacaatg tcggaaatgg ccgccattat
       61 caacggcgaa ccaaaaatat tggaaaacaa agaaggatcg cggctgacgc cgtcggtggt
      121 ggcgctcacc aagaccggcg agcggctggt gggggtgctg gccaagcgcc agcaaatcac
      181 caatccgaaa aacaccttgt tttcggtgaa gcggctgatc gggcggcggt ttgccgacca
      241 ggaagtgcaa aaggatttga aacttttgcc ctatgagatg cgcgaacgcg cgcaggacgg
      301 cggcgtggaa gtgaagatgg gggacaagtg gtatgcgccc gcggaaattt cggcgatggt
      361 tttgcaaaaa atcaagcagg atgccgagtc aaaattgggg gaatccgtaa ccggcgcgat
      421 catcacggtg ccggcctatt ttgacgactc gcaaagaaag gccaccaaac tggcgggcga
      481 gatcgccggc tttgaggtga agcggattat caatgagccg acggccgcgg cattggctta
      541 cggattgacc aaaaagaaga acgagcaggt gctggtctat gatttcggcg gggggacctt
      601 tgacgtttcc attcttgaca tttccgaaga taccgtggaa gtgaaagcga ccggcggcga
      661 caatcatttg ggcggcgacg acgtggatca gcggattatg gattggctgg tggccgaata
      721 tcaaaaagac aacggggttg atttgtccaa agacgaactg gcgcttcaaa gattgaaaga
      781 ggccgctgaa aaagcgaaaa ttgaactgtc cacggcgatg gaaaccgaga tcaatctgcc
      841 gtttatcacc agcgacgctt ccggacccaa gcacttgtat tacaagctca gccgcgcgaa
      901 actggaagac ttggtgcgcg atttgattga acgctccgtc aatttggtgc gccagacttt
      961 gggcgatgcc aagatggcgc cgcaagacat tgaggaaatc gttttggtgg gcggccagac
     1021 gcggatgccg ataattcaag aagaagtgaa gaaatttttc ggcaaagagc ccaacaaatc
     1081 catcaatccc gacgaagtgg tggccatcgg cgcggcagtg gaggcgggaa ttctgcaagg
     1141 cgaggtgaaa gatgttttac tgctggacgt gacgccgctt tctttgggga ttgaaacgat
     1201 ggggggcgtg aacacgattt tgatttccaa aaacaccacc gtgcccacgg ccaagtcgca
     1261 ggtcttttcc accgccgccg acaaccaaac ttcggtggag attcatgttt tgcagggcga
     1321 gcggccgatg agcgcggaca ataaatcttt gggcaaattc attttggacg gcattatgcc
     1381 cgcgccgcgc ggcattccgc aagttgaagt ttcgtttgac attgacgcca acgggatctt
     1441 gagcgtgacc gccaaggaca aggcgaccgg aaaaacccag agcgtccgca ttgaaggctc
     1501 ggcgggaatt tccaaagagg aggccgagcg gatgaagcag gaagcgcagg ccaatgccga
     1561 aagcgatcgc aaaaaacagg aattgattac ggcgcgcaac aacgccgaca acctgattta
     1621 taccgcgcaa aaagcaatgc gcgatgcagg cgacaaagtt tccgctgacg ccaagaaaga
     1681 aattgaagaa aagattgagg gcttgaaaaa agtccagagt ggggataata tggaggagtt
     1741 gaaggccaag tccgacgatt tgtcgcaaac gctttccaaa atcggcgcgc agatgtatca
     1801 acagcaatcc ggcgcgggaa cgcaatccgc cggcaacgaa gcggcgggtt ccgccggaga
     1861 cggcaaaaag aaagacgaag gcgccgcgga aggggaatat caggaaaaat aataaaataa
     1921 atcccaagaa ccaaattcca agctccaaat aatattcaaa tctcaatatt aaaaatttaa
     1981 atattgaata ttgaaattta ctttgttatt tggtgcttgg ttcttggaat tttaaattat
     2041 cgtgagtaag gattactaca aaattctggg agtgcaaaaa gaggcgacgg ccgacgagat
     2101 caaaaaggcc tatcgcgctt tggcgcataa atatcaccct gataaaggcg gcagcgccga
     2161 aaagttcaaa gaggttaacg aggcctacca ggtgctttcg gacaaacaga agcgccagca
     2221 atacgatcaa ttcggcaatg cgtttgacgg taaaggattt tcgggattcg gcggcgcgca
     2281 agcggggcag gacggctggt tttggggacg gcccggcgcc gagtttgacg tgcagtttga
     2341 agatttgggc gatattatgc aggagatgtt cggttttggc caaccgcggc ggcggcgcga
     2401 tggcaagcgc ggcaaagaca ttcgcgtgga cgccgaaatt accctggaag aggcgttcac
     2461 gggcgcgcaa aaagaattca atatttccaa gaatgtcgct tgcccccgct gttcgggaag
     2521 tggcgccgaa cccggcacca aaatcaagga atgtttttcc tgccgcggca cggggcaggt
     2581 tcagcagatc aaaaaaacct ttttggggtc cttcaccaaa tataccgttt gtccggaatg
     2641 ccgcggcgaa ggctatcgcc ccgaaaaacc ctgcaatgtg tgccaagggg aaggccgggt
     2701 aaaaaccgaa gaaacaatca aggtggacat ccccgccggc gtggacaaca atcaggtaat
     2761 taaattggag caaagaggcg aggccggccg gaaaggcggg cccgccggag atttctatgt
     2821 gcgggtgttt ttgaaagcgc atccggtgtt tgaacgcaaa ggcgacgacc tttatatttt
     2881 gcgcgaaatt ccgctgtctt tggcggcgtt gggaggggag gttggcgcgc ccacgattga
     2941 aggaaaagat atcctgctga aaattccttc cggtaccgag tcggggaaaa ttttccgcgt
     3001 ttcgggcaaa ggtatcacgc gtttcaatgg ctttggccgc ggaaatatgt atgtgcaagt
     3061 tgagatcgcc gtgcccaaaa aactgaacaa aaaacaaaaa gagattctgg agcatttgcg
     3121 caaggagggg ttgtagccgc caaaggcgga gttgccaatc ttcaatttcc aaattccaaa
     3181 aattaaaaaa ccggcggcgc cggttttttt ggtctgtccg gcgagcggcc gctttcggga
     3241 taaccgttca ctgggattta aaattaaagg ggtctatccc tcgttactgc gagggttaga
     3301 cccctttaat tccgcgctta aaattctttt gccagggtct aaccgctcgc gattatccct
     3361 cggattacct cgggacaagc gcgagggata gacccttaaa atacaatccc cagtgaatga
     3421 ttactctcgg ggagatggct ggtttttgaa atttgcttta taatctaatt ataatttcta
     3481 ctttagcgat gaaaaacaca cattttattt tcaagacgat ttttgttgcg agcttgtggt
     3541 taatgtttgg ctttttgaat gtcgccggtg cggccaacta cgagtgcggc gcggcggggg
     3601 aagtgcaagg atgccgggtt tgccgacaaa cattggggag cggcggcaat cttgcccatc
     3661 attgggcgga cgacaccacg cggtgcgccg tcgggcaggc gtgcgttgcc ggcgcgtgtg
     3721 ttgacggcca gccgcttttg ccgccggttt acgatgttac ggtggttaaa tctcaagata
     3781 cggcaatcgt aaaagattcg ggcggaaata taattttttc cggaaacgat tacactcaaa
     3841 ttcttcgcga cgccattgcc gcgaccccgg accatggatc gctgtatatc ggtgccggcg
     3901 tttatgacgg attgcgcgcc gaccaaaaca ttccgatcaa caacgctctt tccggatcgc
     3961 aagcgcatta ttacaccgcg cttcctttgc gggggaaaaa tatccacatt tacggcgcgg
     4021 gtatggggca gactgttttc aagcttggcg ataaccaata ttattccggc catcaggcgc
     4081 tgatattttt tgccagcaat ggtttggggc tgggatatac ggggcttacc ttggcgaata
     4141 tgacgctgga cggcaacaag gataagcagg cgccgttttg gtatgacggc gccggattga
     4201 ttcttaccgg cggcgaaaga accggcggca aatttttcaa tttggaattg caaaattctc
     4261 cgaacaccgg cttgtatctg ggcaacaacg gcagtggctg ggaagcttac gcttatctgg
     4321 acaatatcta cagccatgat aaccggcagg cgggagtcgt ttttgacaat ggcgaaaaaa
     4381 taagggccag ccgcttgaaa ttcgtcaatg acggcaccgt tgcttccggc acggtggcgc
     4441 ttcaaatcat taacgtgggc gaccataaac ttgatttggc gattgacggc gccgaaatca
     4501 ataacggaat attaaaaatc ggcaacgagg aatatccggg agccggcggc attacgataa
     4561 gcaatttgaa gattgatacc gatatctcaa atcttgggct tatcggcgcg atatacatca
     4621 aaaacaatca cgattctccc tattcgctgg ccgaccgcgg agagattaaa atcggaataa
     4681 gttcaataac cacggatacg gcttcaaaaa atcatgcggt ttggattgag caagaaccga
     4741 taccggtggt tttgagcggg ggattgattg atgccggcgt tgatgtttat gccaatcggg
     4801 caaaatcggt gagccttgaa ggattgcgcc ttaaatcaag ctttaattct ctgattgcgc
     4861 aagattccaa agtttattgc aataattgca tttttgagcc ggcaaaagag caatacatgt
     4921 acagtatcgc cggcgccggc gcggaaattc atttcaataa ttcaacggcg gtgtctctgg
     4981 aaaaaattga tgtatccgcg gggaaggtat atggtgaaat aacgcttaat acgggtgctt
     5041 gcaacgacga atgttctcct tccggggcca aaaggtgcgc ggccggcggg gtgcagatat
     5101 gcggttcttc gggaggttgt ttgaaatggg gaacggaggc cgtctgtccg tccggacaaa
     5161 catgccgcga cggcggttgc gtcgttgccg cgtgtactcc cgagacctgt gcatcttcgg
     5221 gatatgattg cggcgcggtt tccgacggtt gcggcggcaa tcttgattgc ggcggttgcg
     5281 gcggcggcaa aacatgctcg gacggaaaat gcgtttccgg ttgcgtgccg caaacggtca
     5341 gaaaatgcgg cggcgacgga aatttgtatt ggtatgattc atgcggcgtt caaggagaat
     5401 tggcgcaaag ttgcggcagt gatgaattga cttcaaatta tcgctgttcg ggaaatcggg
     5461 tccagcggca aatattgaag cggggttgcc tggcggatgc ctgcttcacg gccccggaat
     5521 ggcaagatgc ttttgattgt tccgctggcg aagcttgccg cgacggcgat tgcgtcgctt
     5581 cggaaacgga ttcctcttcg gtccagtcgg cggatggggg agtcgccaag atattcagtg
     5641 tgacctatga aaaatatcgg gtctggatag tggccaaaat tgagggaatg agattgcgct
     5701 ttattgaaat aacgcgccag ttttttgaca agttttaagg ctacgccggg aatatcgcga
     5761 ccgaggaaat ttgaaatacg ggataagatg cgggctgcga aagcctcctt attcgtggtg
     5821 cccctggggg gaatcgaacc ctcgttagct gcttaaaagg cagttactct accattgagt
     5881 tacaggggcg ggatgccttt tccgcttgtg cggaaaagaa ttttcaatta ccaattttca
     5941 attttcaaat ataaactcaa aatcaaaatt gccaacaacg ggagtatagc atcttttatt
     6001 atttttgtca tccccaaagc gctgcaaaaa ttattttttt atcgcaaaaa ggggtgatgt
     6061 tatatttatg ctatgtataa atatagcatt tggcgcgagg aacgagccgg atggcggcga
     6121 agcctttgat ttttcatatt tcatacggaa cattgcggac aatgcggaaa aattgataaa
     6181 aaaggcaaaa aagattagaa tcaaattgtt aaaaacgctt ccgaaacaat cggaaggcgc
     6241 tccaaaaaac aaaattatga aaagaaaaaa cattttcaaa ttgattgcca gtatcgtgat
     6301 ttgccaggcg gcggcgctga tcggatcaat gttcacaatg ccggcggtcg gaggctggta
     6361 tgcggaatta aacaagcctt cgtggacgcc ttccggcgga gtcatcggcg cggtgtggac
     6421 ggtaatctat cttttgatgg gggtttcgct ttatctggcg tgggccaaaa atttccaaat
     6481 ttcggaaaat ctttcgggaa tggccgcgcg cgcgtggaat tgcatttcgc aaaaattgct
     6541 tgatggcgcc tggcaaaaac aaaatatcat cgcggtcttt tcggtgcagt tgattttaaa
     6601 tgttttgtgg tcgtttgttt tttttgaatt gaaaatgccc ggatgggcgt tttttgaatt
     6661 gctgatgctt tgggttgcgg ttgcttacac gattgccaat ttccggcgcg tgtccaaacc
     6721 ggcggcatat ctgctgttgc cctatatcgc gtgggtgact tttgccggct atcttaattt
     6781 tgtcatctgg cagatgaact gattgcttaa acgatccgat ccaaaaattc cgttggagga
     6841 gtaatcctta ttttctatgg caaacacaat aaaaaaaata ttgtctttga tattgccgct
     6901 tgttattgcc gcggcgtttg cgccgttggt ggttttagcg cagaatccgg tcaatgttta
     6961 ttttttctgg gggcaggggt gtccgcattg cgagcgcgaa aaagaatttt tggccgatct
     7021 ggcggcaaat cgtccggaaa tcttgataaa ggactttgaa gtgtggggaa acgccgaaaa
     7081 tcgccgcatt ttgggagagt tgggaaagga attgaatgtt gatgtttccg gcgtgccgtt
     7141 caccgtgatt ggcgataaat attttatcgg ctggtatgac cgcgcctcca ccggtgcggc
     7201 gattgaagcc gccgtggcga cggcggttga gcaaggctgt cgcgacgttg ccgcgccgat
     7261 cattgatccg gaagaaactg ccgcgccaaa caacggcagt tgcggaaata acggcaaaac
     7321 ggaaattccc gaaaaaataa atgcgc
//
LOCUS       DAOQWE010000027         7342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_10515_length_7342_cov_7.156031, whole genome shotgun sequence.
ACCESSION   DAOQWE010000027 DAOQWE010000000
VERSION     DAOQWE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10515_length_7342_cov_7.156031"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="rplL"
                     /locus_tag="PKK37_01695"
     CDS             complement(<1..310)
                     /gene="rplL"
                     /locus_tag="PKK37_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNR81127.1"
                     /translation="MDEKKEVQVPEKFADLVKKIEEMSVLDLAELVKILEEKFGVSAA
                     APMMMAAPAAGGANGAPAAEEQSSFTVVLAAPGDKKIEVIKAVKEITQKGLKEAKDLV
                     D"
     gene            complement(423..944)
                     /gene="rplJ"
                     /locus_tag="PKK37_01700"
     CDS             complement(423..944)
                     /gene="rplJ"
                     /locus_tag="PKK37_01700"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNR81128.1"
                     /translation="MPITKQKKREIIKELADKLDRQKAAVFADFSGLKVKDLTDLKSK
                     LRQKGAEFKVAKKTLMNLALKQKGIDADTKTMAGEIAVAMGYDDEVSAAKELYQFSKT
                     NQNIKILGGLLENKLIDAAQVLSLAQLPSKLELLARLTGSLAAPSRNFAGVLQGNLRG
                     LVQVLSQIQKSKA"
     gene            complement(1115..1486)
                     /locus_tag="PKK37_01705"
     CDS             complement(1115..1486)
                     /locus_tag="PKK37_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR81129.1"
                     /translation="MKNDKEKFKTEFKLRIYRWVLDLVKFLDGLPKDIISQKMVDQLF
                     RSGGSIGANYIEAQASSSKKDFINFLHHSLKSSNESKFWLAVLRDTGRADRKKADCLL
                     QELTEISKMLGASLLTLKGKR"
     gene            complement(1510..2616)
                     /locus_tag="PKK37_01710"
     CDS             complement(1510..2616)
                     /locus_tag="PKK37_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81130.1"
                     /translation="MKIENDPMDKETFFTEYKGVFVAIAAAAILGLIFLANFFFSSKK
                     ISLPPGEPSLTAGQSYEIEWSSSNVDRVGIVLFGGDQPQWIAYNYPAGAQKFVWQSDP
                     YQSAGSQYRFAVFEYPWKKGNAIAYSPAMITILGQKYVSCDSYSVEQNWPYLPSKHAG
                     LLRAFITQNTYSGDLGGLPGADEKCNQEAKKAGYEGNYIALLGTDDISARERVQKDGI
                     FAEAGAVGQAVEGQACHRYLAQNIQKLLDKANLSREAAQIQFGASFNQSFGNLWLGRM
                     APAIEKKCLALGVDREGEMMFSNSYTCQDWTVGKRAISGVADANLMRCYDLQGKNLVA
                     KYYGAAAIALGEQGGYALGAASCDTKRRLLCVEQ"
     gene            complement(2712..3059)
                     /locus_tag="PKK37_01715"
     CDS             complement(2712..3059)
                     /locus_tag="PKK37_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81131.1"
                     /translation="MEIIKQIPTAFYLSLVILPIAVWGFFVASKPKKTISAKNADPAE
                     QLKAKKVNLKKEIQSGLLLGTAIAAGMGLFISLGMIIGDYKNDRGMFAAVLPPLSLPP
                     APEAVLPPAPRAE"
     gene            complement(3068..5146)
                     /locus_tag="PKK37_01720"
     CDS             complement(3068..5146)
                     /locus_tag="PKK37_01720"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HNR81132.1"
                     /translation="MTIPVPRPAQKRKRKVHKEPVNFRLAILILAFFSFGLVCYLAAS
                     YRQPSIPLRVIGGVPGASTAAEGGGAGEMKSGGLERFSSQDDFKSYLDSGRRAAAAGP
                     FEAEKAALTAKEETPATKPAAKESAPAPAGITPEAAKKFFSLGEASAKSAGDIIRLDG
                     GKLYFSPQNQFYWPEFATATRPAGETKIFETRQTGEMKQIGVIPQDGNFLVLDGSLAV
                     FLNNSLAVYDLSFADSPLEIWQGRLNGGSRIIASQFSAGKLYLAVETEIDPANPCPIK
                     PLVIGESPVSVECASIYRPAAAVPADLIFTIFEMNPRSGAVARDFSVAVPRDGFSTIL
                     EDGAAWVAWSEERDHVEFFADFLSAKCRGLLPNYIVEKTRSTAACEISRAGKEFETRR
                     LLAGWFDSLAQGEKERIAGEISLRLADYLRDKTKDFERTGIAKINLETFAPEAQTAVA
                     GRLPEGGLAPAAGGLRAVTVSGFGAAQKMEWLVSGNIAGQENEKAQNNIYSLDKNLKT
                     VAEAKNLDIPAGVCALRFSANAAYARVCRAGEPLYALSFNPQAAGLAGKFSLPAEDAY
                     FYPLNEISAIAVYPNARKIKVALFDSSLPGRIEKIHEYALNDYWADFEANIAAFAHDP
                     ENKRLFLPGARGGNILSYAGSEITLTGSAGEIAPARAFFEKGDLYIAGDAGIEVFGGA
                     DFEKINSVEF"
     gene            complement(5427..6659)
                     /locus_tag="PKK37_01725"
     CDS             complement(5427..6659)
                     /locus_tag="PKK37_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR81133.1"
                     /translation="MVNNLGEYFKIAARNLKMRSLRSWLTVLGIIIGVFLIISLLSLS
                     EGIKETINRQMMSLGGNMIFVMPGDDSNPLAGMMFGGDKLEARDLDAIRRTEGVKTVL
                     GASYQSINVRYNTENKALTVEGFPLVEGADVLRKFQGWSLSSGRWPSASRREVVVGMQ
                     FGRDIFNAPVQPGDQLIIKGKKYTVAGILNSLGAKDDDSAIFMDIAEFQDLTGQQRGT
                     AQIAMVQIDEAMDPNLIAQKIKDSLRRVQRRAYGSDDLHFAVITAEKVNDIVGGVMAV
                     IQFAILAFAGIAILVGGIGITNTMYTSVRERTREIGIMKAIGATNGAIMSIFLFEASI
                     IGFGGGIGGTVLGLGLAAIIQAYGQVHPLFYFTASFSPWIVIFGLGFSFLVGCLSGIL
                     PARQAAKLRPIEALRKYE"
     gene            complement(6695..>7342)
                     /locus_tag="PKK37_01730"
     CDS             complement(6695..>7342)
                     /locus_tag="PKK37_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR81134.1"
                     /translation="KTEVNSLRGVNLEIESGDFVTIMGPSGSGKSTLLNMIGLLDIPT
                     KGRLLLKNKDVTKRTESELAQLRGKTIGFVFQEFNLLNHLRAWENVELPMIFQKIPQA
                     IRRRRAMELLAQVNMANRADHQLAELSGGERQRVAIARAFANNPELVIADEPTGNLDS
                     VTGQKVMEILLDFHEKMGGTLLVVTHDHNIGGYAEHQVFIKDGRIVSQDEAFGNK"
BASE COUNT         1730 a         2072 c         1682 g         1858 t
ORIGIN      
        1 cgtccaccaa atctttggct tctttcaatc ccttttgggt gatttccttc accgccttga
       61 tcacttcaat cttcttgtcg ccgggagcgg ccaaaaccac cgtgaacgaa gattgctctt
      121 cggccgcggg ggcgccgttc gcaccgccgg ccgcgggggc cgccatcatc atgggcgccg
      181 cggcgctcac gccgaatttt tcttccagga ttttgaccaa ttcggccaga tccagcaccg
      241 acatttcttc aatttttttc accaagtccg cgaatttttc cggcacctgg acttcttttt
      301 tttcatccat aaaattttta atttttaatt tttaatttcc aaacaatttt aaaattttaa
      361 aatttaataa ttaaaaattg tttaaaaatt tcaaatttaa aatttcaaat tgatttttaa
      421 ttttatgctt ttgacttttg gatttgcgat aacacttgca ccaggccgcg caggttgcct
      481 tgcagaacgc cggcaaagtt tcttgacggc gcggccagac tgccggttaa tcgcgccagc
      541 agttccaatt tggacggcag ttgcgccagc gataacactt gcgccgcatc aatcaacttg
      601 ttttccagca aacctcccaa aattttgata ttttggttgg ttttggaaaa ttgatacaac
      661 tcttttgccg ccgacacttc gtcgtcatag cccatcgcca cggcgatttc ccccgccatc
      721 gttttggtat cggcgtcaat gcctttttgt ttcaacgcca aattcatcaa ggtttttttg
      781 gccaccttga actccgcgcc tttttggcgc agtttgcttt tgagatccgt caaatccttg
      841 actttcaggc cggaaaaatc ggcaaacacc gccgccttct ggcggtccag cttgtcggcc
      901 agctctttga taatctcccg cttcttttgt ttggtgatcg gcatcttttt ttgtaaacaa
      961 aaaatccgct cgcgcagact cgactttgaa cgaaaaatca acgattgatt ttcctcggcg
     1021 gggcttattg atcattgatc ggcccgctgt ctgcggaatg cttcaaatat atcaaagttt
     1081 gagaaattgt caacgtttca aattaaaaag tcatttacct cttgcccttt aatgtgagca
     1141 ggctggctcc cagcattttt gatatctccg tcaactcttg caacaaacaa tccgctttct
     1201 tcctgtcggc acgccctgtg tcgcgcaaca ccgccagcca aaatttggat tcgttgcttg
     1261 acttgaggga atggtgcaga aaattgataa aatctttttt tgagcttgac gcctgcgctt
     1321 caatataatt ggcaccgata ctgccgccgc tccgaaacaa ctggtcaacc atcttttgcg
     1381 aaatgatatc tttgggcaac ccatcaagaa atttcaccaa atcaagaacc cagcgataga
     1441 tgcgtaattt gaattcggtt ttaaattttt ccttgtcgtt tttcattttt cattctcaat
     1501 ttttaatttt tactgctcca cgcacagcaa gcggcgtttg gtgtcgcagg aagcggcgcc
     1561 gagggcataa ccgccctgtt cgcccaaagc gattgccgcc gcgccgtaat attttgccac
     1621 cagattcttg ccttgcagat catagcatcg catgagattg gcatcggcca cacccgagat
     1681 cgcgcgcttg cccaccgtcc aatcctggca agtgtagctg ttggaaaaca tcatttcccc
     1741 ttcgcggtca actcccagcg ccaagcattt tttttcaatt gccggcgcca ttctccccag
     1801 ccacaaattg ccgaaacttt gattaaacga agcgccaaac tggatttgcg ccgcctcgcg
     1861 ggatagattg gccttgtcca gcagtttttg gatgttttgc gccaaatagc gatggcaagc
     1921 ttgcccttcc accgcttgcc cgaccgcacc ggcttccgca aaaatcccgt ccttttgcac
     1981 ccgctcccgc gcggaaatat catcagttcc caaaagcgcg atgtagttcc cctcatagcc
     2041 cgcttttttt gcttcctgat tgcatttttc atccgcgccc ggcaatccgc ccaaatcgcc
     2101 cgaataggtg ttttgcgtga taaacgcccg caaaagcccg gcgtgctttg acggcagata
     2161 gggccagttt tgctccaccg aataactgtc gcaggaaaca tatttttgtc ccagaatcgt
     2221 gatcatcgcc ggactgtagg cgatggcatt gcctttcttc cacggatact caaacaccgc
     2281 gaaccgatat tgactgccgg cggactggta gggatcggat tgccaaacaa atttttgcgc
     2341 gcccgccgga taattgtagg caatccattg cggctgatcg ccgccgaaca acacaatccc
     2401 cacgcgatca acgtttgacg acgaccattc aatttcatag gattggccgg cggtcaaaga
     2461 aggctctccg ggcggcaggg aaattttctt tgaagaaaaa aagaaattgg ccaaaaagat
     2521 caaccccaaa atcgccgcgg cggcgatggc gacaaagacg cctttgtatt ccgtgaaaaa
     2581 agtttcttta tccatcgggt cgttttcaat tttcataata ttgattttta atttttgtcg
     2641 cggtatcgta gcggtatttg atatcgctaa attttagcga tatcaaatac cgctactccg
     2701 gcgccgcccc gtcattccgc ccgcggcgcg ggcggcaaaa ccgcctccgg cgcgggcggc
     2761 agggaaagcg gcggcaaaac cgcggcaaac attccccggt cgtttttata atcaccgata
     2821 atcattccca atgaaataaa aagtcccatt cccgcggcaa tcgccgttcc caaaagcaat
     2881 cccgattgaa tctctttttt gagattgact tttttggctt tcagctgttc ggccggatcg
     2941 gcgtttttgg ccgaaatcgt ttttttcggc ttggaagcaa caaaaaatcc ccaaaccgcg
     3001 atgggcaaga tcaccaatga aagatagaag gccgttggaa tttgttttat gatttccaaa
     3061 atttccatta aaattcaacc gaatttattt tttcaaaatc cgcgccgccg aaaacctcaa
     3121 ttcccgcgtc cccggcaatg taaagatcgc ccttttcaaa gaacgcgcgg gccggcgcaa
     3181 tctcgccggc gcttcccgtg agcgtgatct cactgccggc gtatgacaga atgttgccgc
     3241 cgcgggcgcc gggcaagaac agccgcttgt tttcgggatc atgggcgaac gcggcgatat
     3301 tggcctcaaa gtccgcccaa taatcgttga gcgcatactc atgaattttt tcaatccgtc
     3361 cgggcaggga ggagtcaaac agcgccacct tgatcttgcg ggcgttcgga tagacggcga
     3421 ttgccgaaat ttcgtttagc ggataaaaat aggcgtcttc ggcgggcaaa gagaatttcc
     3481 ccgccaatcc cgcggcttgc ggattgaagc tcagcgcata gagcggttcg ccggcgcggc
     3541 aaactcgcgc gtaggcggca ttggcggaaa aacgaagcgc gcaaaccccc gcgggaatgt
     3601 ccaaattttt tgcttcggca accgttttta aatttttgtc cagcgaataa atattgtttt
     3661 gcgccttttc gttttcctgg ccggcaatgt ttccggaaac cagccattcc attttttgcg
     3721 ccgcgccgaa tcccgaaacc gttaccgcgc gcagtcctcc ggcggcggga gcaagcccgc
     3781 cttccggcaa gcgccccgcg acggcggttt gcgcttccgg cgcgaaagtt tccaaattga
     3841 tcttggcgat tcccgtgcgc tcaaaatctt tggttttgtc ccgcaaataa tccgccagac
     3901 gcaaagatat ttcacccgcg atccgttctt tctcgccttg tgccagagaa tcaaaccaac
     3961 cggccagcaa acggcgcgtt tcaaactctt tccccgcccg cgaaatttcg caagccgccg
     4021 ttgaccgcgt tttttccacg atgtaattgg gtaaaagtcc ccggcacttg gcgctcaaaa
     4081 aatccgcgaa aaattcaacg tgatcccgct cttcgctcca cgcgacccac gcggcgccgt
     4141 cttccaaaat cgttgaaaaa ccgtctcgcg gcacggccac ggaaaaatcg cgcgccaccg
     4201 ctcccgaccg cggattcatc tcaaagatcg tgaaaattaa atccgccggc accgccgcgg
     4261 ccggccggta aattgacgca cattccaccg acaccggact ttcgccgatc accaagggct
     4321 tgattggaca gggattggcc ggatcaatct cggtctccac cgccagataa agcttgccgg
     4381 cagaaaattg ggaggcaatg atgcgactgc ctccgtttag ccgtccctgc caaatttcca
     4441 gcggagagtc cgcgaaagaa agatcgtaga ccgccagcga gttgttcaaa aacaccgcca
     4501 gcgatccgtc cagaaccaga aaatttccgt cttgcggaat cacaccaatt tgtttcattt
     4561 cgccggtttg gcgcgtttca aaaattttgg tttcacctgc cggccgcgtt gcggtggcaa
     4621 actccggcca atagaactga ttttgaggtg aaaaataaag cttccctccg tccagccgga
     4681 tgatatcacc ggcgcttttc gcggacgctt cgcccagtga gaaaaatttt tttgcggctt
     4741 cgggcgtgat cccggcaggc gccggcgcgg actctttggc cgccggcttg gtggcgggcg
     4801 tctcctcctt ggcggtaagc gccgctttct cggcctcaaa cggccccgcg gcggcggcac
     4861 ggcggccgga atcaagataa ctcttgaaat cgtcttgcga tgaaaatctt tccaatccgc
     4921 ccgacttcat ttcaccggcg cctcccccct ccgcggccgt ggatgccccc ggaacgccgc
     4981 cgataacccg caagggaatt gatggctggc gatagcttgc cgccaaataa cagacaagtc
     5041 cgaaagaaaa aaaggccaga atcaaaattg ccagccggaa gttaaccggc tctttgtgga
     5101 ctttgcgttt tcttttttgc gcgggacgcg gcacgggaat cgtcataatg aaaaattgaa
     5161 agattggcga ttgtttccgt ctttatttta cgaaaaaaaa tccgccggcg caaggagcaa
     5221 aatttaaaaa tgaaaattta aaaagtaaaa acgaaaatta aaaactaaaa acttatcttt
     5281 tgccctttaa agttaaaagg cttgcgccta gtatttttga aattttgaca agctctcaca
     5341 ataaagattc aattttatct ttatcgctgc ggttttaaat ttttctttgt cgtttttcat
     5401 ttttcattct cagattttaa tttttatcat tcgtattttc tcagcgcttc aatgggccgg
     5461 agtttggccg cctggcgggc gggcaaaatt cccgaaagac accccaccaa aaacgaaaat
     5521 cccaggccga aaataacaat ccacggcgaa aacgaggcgg tgaaatagaa cagcggatgc
     5581 acctgcccgt aggcctgtat tatcgccgcc aatcccagcc ccaaaaccgt gccgccgatg
     5641 ccgccgccga agccgatgat gctggcctca aacagaaaga ttgacattat cgcgccgttg
     5701 gtggcgccga tggctttcat aatgccgatt tcgcgcgtcc gttcgcgcac tgaagtgtac
     5761 atcgtgttgg tgataccgat gccgccgacc agaatggcga tgccggcaaa cgccagaatc
     5821 gcaaattgaa tcaccgccat cacgccgccg acaatatcgt taaccttttc ggcagtgatc
     5881 accgcaaaat gaaggtcatc ggagccgtag gcgcggcgct ggacgcggcg caggctgtct
     5941 ttaatctttt gggcaatcaa attcggatcc atcgcttcgt caatctgcac catcgcgatt
     6001 tgcgccgtgc cccgctgttg gccggtgaga tcctgaaatt cggcaatatc catgaagatc
     6061 gcgctgtcgt catccttggc ccccaaagaa ttcagaatgc cggcaaccgt atatttttta
     6121 cccttgatga tcaattgatc gcccggctgg accggcgcgt tgaaaatatc gcggccgaat
     6181 tgcatcccca ccaccacctc gcgccggctg gccgacggcc atcgtccgga agacaagctc
     6241 catccctgga acttccgcaa aacgtcggcg ccctcaacca gcggaaaccc ctccaccgtc
     6301 aatgccttgt tttcggtatt gtaacgaaca ttgattgact gataggaagc ccccagcacg
     6361 gttttgaccc cctcggtgcg ccggatcgcg tccaaatccc gggcttccaa tttgtcgccg
     6421 ccgaacatca ttcccgccag cggattgctg tcgtccccgg gcatcacaaa gatcatattg
     6481 ccgcccaatg acatcatctg ccgattgatc gtttccttga tgccctcgga aagcgacagc
     6541 aggctgatga tcaaaaacac gccgatgata atgcccaaaa ccgtgagcca cgaacgcaat
     6601 gaccgcattt tcaaattcct ggccgcaatt ttgaaatact ctcctaaatt attaaccata
     6661 attaaaatgt caaaatttcc aatttccaaa tttgttattt attgccgaag gcttcgtctt
     6721 gggaaacaat gcggccgtct ttgataaaaa cctgatgctc ggcatagccg ccgatgttgt
     6781 gatcgtgcgt taccaccagc aatgttccgc ccattttttc gtgaaaatcc aacagaatct
     6841 ccatcacttt ttgcccggtt accgagtcca gattgccggt gggctcgtcg gcaatcacca
     6901 gctccggatt attggcaaac gcgcgggcaa tcgcgaccct ttgccgctcg ccgccggaaa
     6961 gctcggccag ttggtgatcg gcgcggttgg ccatattcac ctgcgccagc aattccatgg
     7021 cccggcggcg gcgtatcgct tgcggtatct tctggaagat catcggcaac tccacatttt
     7081 cccacgcccg caagtgattt aaaagattaa attcctgaaa cacgaacccg attgtcttgc
     7141 cgcgcaactg cgccaattcg ctctcggtgc gcttggtaac atccttgttt ttcaacaaaa
     7201 ggcggccctt ggtgggaatg tccaggagtc cgatcatatt cagcaaagtg gattttcccg
     7261 aacccgacgg ccccataatc gtgacaaaat cgccggattc tatttccaga ttcacccccc
     7321 gcagagagtt aacttccgtc tt
//
LOCUS       DAOQWE010000028         7268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_10651_length_7268_cov_8.132677, whole genome shotgun sequence.
ACCESSION   DAOQWE010000028 DAOQWE010000000
VERSION     DAOQWE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10651_length_7268_cov_8.132677"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..659)
                     /locus_tag="PKK37_01735"
     CDS             complement(3..659)
                     /locus_tag="PKK37_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81135.1"
                     /translation="MKTRIVKIVGAAMLSAVIAFSVSPCNICNAFSRQELLAQVEEVK
                     RQILVLQIQLVQTKIYDLQIQLAQSQFSPAFVDVIWPAGGEKLENRHCYHILWEARGV
                     EKVSVALKTPGSMRIIAENVSANDGNWYWDVGSIAGNDYRIRVFDASNPKIAGETAKT
                     FAIFDNSLKNKCADGTVAGQCSTDKPKLCFDTEIGLIDACKSCGCPSGQYCGTDSKCR
                     "
     gene            complement(661..1512)
                     /locus_tag="PKK37_01740"
     CDS             complement(661..1512)
                     /locus_tag="PKK37_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNR81136.1"
                     /translation="MAQNNQEISSLSTIVVLIVMAVAFLPAAAKIFGPVAPVMVIAGL
                     ILVILLIGLRVIDQYERGIVLTLGQYSGTRGPGLTWILVGIQRMIKVDMRITTVDIPS
                     QEVITKDNVPVGINAVVYFQVESAEKSILNIKDYTLAVAQYAQAALRDVIGGIELDPL
                     LSEREKISEEIKKIVDTATDSWGINVADIKIQDIELPADMKRVMAKQAESERERRAII
                     IRAEGEFQASARLAEAAQVLNGTPGGLSMRTLQTIEKINPDPSKTVIFALPVEIMEGF
                     KKITGGK"
     gene            1556..3697
                     /gene="recG"
                     /locus_tag="PKK37_01745"
     CDS             1556..3697
                     /gene="recG"
                     /locus_tag="PKK37_01745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNR81137.1"
                     /translation="MNLEIPIENFTRISSAYQARLKNLGILTMRDLIYHFPFRYQDFS
                     ARADIADLEAGKTVCVQGEVQSVKIIRIWKRHMTIVEAIVKDGTGQVKAVWFNQPYLA
                     STFKKGIVVCLAGKLAEKRGAKYLSNPMYEKVRDAGGGEDDYTHTGRLVPVYPETAGV
                     SSRWLRYIIKPLLGQAAKIPDPLPKTILEAYDFMPFEEAIKQIHFPDSEDLAARARKR
                     FAFEELFFIQLAVLRQRLRLNRETARAIPFDEKLIKKFVQSLPFSLTDAQRKTAWQIL
                     KDMQRDHPANRLLQGDVGSGKTIVAMIAALAAAKSGGQTAVMVPTEILANQHYESFSK
                     LLCPFGVEIGILCAKTCAVSSYGKVSKPAALDLAKSGDTQILIGTNALIQKGVEFKNL
                     DLVILDEQHRFGIDQRATLAKNRQTKKKGQIMPHLLSMTATPIPRTLALTVYGDLDLS
                     LIDQMPPGRKKIETKIILPDDRAGTYEFIRRQIESGQQAFVICPRIDPEEGQPEIKRD
                     GILFDPKADWSGAGAVKAVKEEYEKLSKEIFPELRIGMLHGKMKAAEKQEVMNKMKAG
                     KIDILVSTSVIEVGIDIPNATIMAVEGSHKFGLAQLHQFRGRVGRGEHQSYCLLFSDY
                     PTRRLAAMLKFDSGFHLAEKDLEIRGPGEVFGSQQWGAPDLAMASLADVFLVEKSRAA
                     AKGILQSDPGLSRHPQLRARIAHFSARAHLE"
     assembly_gap    3848..3947
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3948..4727
                     /locus_tag="PKK37_01750"
     CDS             <3948..4727
                     /locus_tag="PKK37_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNR81138.1"
                     /translation="QIGEPLVGRKTTINLYPFAQLELAQEQNAFDLKQKLEDFLLFGS
                     YPNVYTAKTKDEKISALNDIADSYLLKDVLAIDRVKKPKIIIDLVKMLALQIGSEVSL
                     TELAQNLSVNLNTIAKYLDILEKGFVIISCGALRRNLRNEIRGKKKYYFYDNGVRNAL
                     LSQFNGLSLRNDTGALWENFIFTERLKTRAYKNIYANYYFWRTYQQKEIDLVEEYGGV
                     LHGYEFKFTGGAAKPKEFLEAYPQSTFEAINKENYLDFLTN"
     gene            complement(4811..4942)
                     /locus_tag="PKK37_01755"
     CDS             complement(4811..4942)
                     /locus_tag="PKK37_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81139.1"
                     /translation="MPAPESQTRLIINKPLGLWITEKPGEEEVGDIAIYMHSKIKKQ"
     gene            complement(4946..6124)
                     /locus_tag="PKK37_01760"
     CDS             complement(4946..6124)
                     /locus_tag="PKK37_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNR81140.1"
                     /translation="MEIEAKIENPAIKKRLNDLDSDNPRMIKEAVNYLARRDKNKQLK
                     TALRDIVENFRICESLAAIWAIVILGIMRDKEAVYCLLDVFESDEDFTQEAAADALVL
                     IERAHPGSVVPATIEFIERRIDNDPFHAKIFAVGVLGEFVDQISVKNFLIKLFEKDKD
                     IQDFIGSVLVNTKDKGILEILKRSLVFAEQVEDGIAIDEMKWHYYYLANGISYAEKYP
                     ENKLWKQNWEQRWEHLLERVAKGDSEEESDDDTDTEKMEERFKEMERKFKGSELDREF
                     KKELAERDAQEIEPFCLEKWLEIRQRSDFEDEFQRALVLIGAGDIWQVEDIQKAMNES
                     RSPGTIVKTISEKINLPDEKSLTVFFEYVNQLWNNTPREELFGLTPAEKMEIYYLSQE
                     "
     gene            complement(6147..>7268)
                     /locus_tag="PKK37_01765"
     CDS             complement(6147..>7268)
                     /locus_tag="PKK37_01765"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR81141.1"
                     /translation="VAHLESIGKKYIFLSLDNEADEKYFTSQTELIEKIKIHLAPGSF
                     VFIDEIQRKKDAGIFLKGIYDLDLPYKFIVSGSGSLELKEKIHESLAGRKRIFEINPV
                     NFKEFVNFKTDYRHEDRIGSYFEIEKDRTKTFLKEYLNFGGYPRVILASHVSEKNKIM
                     GEIFRSCVEKDLLSLLEIDRPEAFSLMIKIIASQSGQILNYSKLGQQIGLTFAPLKKY
                     LWYAQKIFLLRIVRPYFTNKQKEITRSPVAYFHDLGLRNFSIELMGVLSDPQSFSFVF
                     QNFVLNILREKEKNSAATINFWRTIAGAEVDFVINRGREVLPVEVKYIDLKSPVLGKS
                     LLSFIGKYEPNEAWVVNLSLETEIKHKTTAVKFIPYWKV"
BASE COUNT         1940 a         1830 c         1491 g         1907 t
ORIGIN      
        1 tttcaccggc atttggaatc ggtgccgcaa tattggccgg acggacagcc gcaggatttg
       61 caggcgtcaa tcaagccgat ttcagtgtca aaacacagct tgggcttatc ggtggaacat
      121 tggccggcca ccgtgccgtc ggcacatttg tttttcagag agttatcaaa gatcgcgaaa
      181 gtttttgccg tttcgccggc aatttttgga tttgatgcgt caaaaacccg gatccggtaa
      241 tcgttcccgg caatactgcc gacatcccaa taccaatttc cgtcgttggc cgaaacattt
      301 tctgcgataa tacgcatgct tcccggagtt ttcaatgcaa cggaaacttt ctccactccc
      361 cgcgcctccc aaaggatgtg ataacaatgc cggttttcca acttctctcc gccggccggc
      421 caaataacat caacgaacgc cggcgaaaac tgcgattgcg ccaattgaat ttgcaagtcg
      481 taaattttcg tttgaaccaa ctggatttgc aaaaccaata tctgccgctt tacctcctca
      541 acttgcgcca ataattcttg ccggctgaaa gcattgcaaa tgttgcaagg cgaaacagaa
      601 aaggcaatca ccgccgacag cattgccgcg ccgacaattt tgacaatcct tgttttcata
      661 ttattttccg ccggtgattt ttttgaagcc ttccataatt tccaccggca gggcgaagat
      721 cacggttttt gacggatcgg gattgatctt ttcaatggtt tgcagagtgc gcattgaaag
      781 gccgcccggc gtgccgttta acacctgggc ggcttccgcc agacgggcgg aagcttgaaa
      841 ttcgccttcg gcccggatga tgattgcgcg gcgttcccgc tcggactcgg cttgctttgc
      901 catcacccgc ttcatatccg ccggcaattc aatgtcttga atcttgatat cggccacatt
      961 tatcccccaa ctgtcggtgg cggtgtccac gatttttttg atctcttcgg aaattttttc
     1021 ccgctccgac aacagcggat ccaattcaat gccgccgatg acatcgcgca gagccgcctg
     1081 cgcgtattgc gccacggcaa gagtgtagtc tttaatgttc aatattgatt tttccgccga
     1141 ctccacctgg aaataaacca ccgcattgat gcccaccggc acattgtctt tggtgatcac
     1201 ttcctgcgac ggaatgtcca ctgtggtgat acgcatatcc accttgatca tccgctgaat
     1261 gcccaccaga atccaggtga gccccggtcc gcgagtgccc gaatattgcc ccagggtgag
     1321 cacgatcccc cgctcgtatt gatcaatcac ccgcagtccg atcaacaaaa ttaccagtat
     1381 caatccggcg ataaccatca ccggcgccac cggcccgaaa attttcgccg cggcgggcaa
     1441 aaaagccacc gccataacta tcaaaaccac aattgtggac aaagagctga tttcttgatt
     1501 gttttgcgcc atatttttgt ttatttttta ttgcatccat tgtagcatta aagatatgaa
     1561 tctggaaatc ccgattgaaa attttaccag aatctcctcg gcctaccaag cccgattgaa
     1621 aaatctgggg attttaacga tgcgcgattt aatctatcac ttccctttcc gttaccagga
     1681 tttttcggcg cgcgccgaca tcgccgatct tgaagccggc aaaaccgtgt gcgtccaagg
     1741 cgaagtccag tcagtgaaaa taatccgcat ttggaagcgc catatgacga ttgttgaagc
     1801 gattgtcaaa gacggcaccg ggcaagttaa agcggtgtgg ttcaaccaac cctatttggc
     1861 gtcaacgttc aaaaaaggaa tcgtggtttg cctggcggga aaactggcgg aaaaacgagg
     1921 cgccaaatat ctttccaatc cgatgtatga aaaagtgcgc gacgcgggcg gaggcgaaga
     1981 cgactacacg cacacgggac ggctggtgcc ggtctacccg gaaaccgcgg gcgtttccag
     2041 ccgctggctg agatacatca tcaaaccgct tctgggacag gccgcaaaaa tacccgaccc
     2101 tctgccaaaa accattttgg aggcgtatga ttttatgccg tttgaagaag caatcaaaca
     2161 aatccacttc ccggattccg aagatctggc ggcgcgggcg cgcaaaaggt ttgcttttga
     2221 agaattgttt ttcatccagc tggcggtttt gcgccagcgc ctgcgcctca accgggaaac
     2281 cgcgcgcgcc attccttttg acgagaaact gataaaaaaa ttcgtgcaat cactgccgtt
     2341 ttcgctcacc gacgcccagc gcaaaaccgc ctggcaaatc ttgaaagata tgcagaggga
     2401 ccatccggca aaccggcttt tgcagggcga cgtcggttcg ggaaaaacca ttgtggcaat
     2461 gattgccgcg ctggcggcgg ccaaatccgg cgggcaaacc gcggtaatgg ttcccaccga
     2521 aattttggcc aatcaacatt atgaaagctt ttccaaactg ctatgccctt tcggcgttga
     2581 aatcggcatt ctgtgcgcca aaacctgcgc ggtttcctct tatggcaagg tgtccaaacc
     2641 cgcggcattg gatttggcaa aaagcggcga cacgcaaatc ttgatcggca ccaacgcatt
     2701 gatacaaaag ggcgtggaat tcaaaaattt agatctggtg atattggacg aacagcatcg
     2761 tttcggcatt gaccagcgcg caacgttggc aaaaaaccgg caaacaaaaa agaaagggca
     2821 aattatgccg cacttgctct ccatgaccgc cacccccatc ccccgcacgc tggcgcttac
     2881 cgtctacggc gaccttgatt tgtcgctgat cgatcaaatg ccgccgggaa gaaaaaaaat
     2941 tgaaacgaaa atcatcctgc ctgatgaccg cgccggcact tacgaattta tccggcggca
     3001 aatagaatcg ggacaacagg catttgtgat ctgcccgcgc attgatcccg aagaaggcca
     3061 gccggagatt aaaagggacg gaattctgtt tgaccccaaa gccgattggt cgggcgcagg
     3121 cgccgtgaaa gccgtgaagg aagaatatga aaaattgtct aaagaaattt ttcccgaact
     3181 gcgcatcgga atgttgcacg gtaaaatgaa agccgccgaa aaacaagagg tgatgaacaa
     3241 gatgaaagcc ggaaaaattg atattttggt ttccacttcg gtgattgaag tgggcattga
     3301 tattcccaac gccaccatca tggccgttga aggatcgcac aagttcggcc tggcgcaact
     3361 gcaccaattt cgcgggcgcg tggggcgcgg cgagcaccaa tcatattgtc tgctgttctc
     3421 cgactatccg acaaggcggc tggcggcgat gcttaaattt gacagcggct ttcacctggc
     3481 ggaaaaagac ttggaaatcc gcggcccggg cgaagtgttc ggcagtcagc aatggggcgc
     3541 gcccgacttg gccatggcct cgctggcgga tgtgtttttg gtggaaaaat cccgggccgc
     3601 cgccaaaggc attctgcaaa gcgacccggg cttgtcgcgc catccccaac tgcgcgcccg
     3661 catcgcccat ttctccgccc gcgcccacct ggaatgatca tctcaggcct gcgcttttgt
     3721 atagtccact ttacaaaata acctgttgtt ttgtatattt taacaccaac tattgcgttt
     3781 tttatgaaat ttgtatatta ttaatataca aatttaatta aaaacaaaca tttatgaata
     3841 taaaccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncaa atcggcgaac
     3961 cgctggtcgg ccgcaaaacc acaatcaacc tctacccttt cgcccaattg gaactggcgc
     4021 aagaacaaaa cgcatttgat ttaaagcaaa aattggaaga ttttcttttg ttcggatctt
     4081 atcccaatgt ctataccgcc aaaaccaaag acgaaaaaat cagcgcgcta aacgatatcg
     4141 cggattctta tttgctaaaa gatgtgttgg cgattgaccg cgtaaaaaaa ccgaaaatca
     4201 ttattgattt ggtaaaaatg ctggcgttgc aaatcggcag tgaagtttca ctcaccgaat
     4261 tagcccaaaa cttgagcgtc aacttaaaca cgattgcgaa atatcttgat atcttggaaa
     4321 aaggattcgt gataatctct tgcggcgcgt taagaagaaa cctgcgaaat gaaatccgcg
     4381 gcaaaaaaaa gtattatttt tacgacaacg gggtgagaaa cgctttgctt tcgcaattca
     4441 atggattgag tttgcgcaac gacaccggcg cgctgtggga aaatttcatt tttaccgaac
     4501 ggctaaaaac ccgcgcctat aaaaatatct acgccaacta ttatttctgg cgcacttatc
     4561 agcaaaaaga aattgatctg gtggaagaat acggcggagt tttgcacggc tatgaattca
     4621 aattcaccgg cggcgcggcc aaacccaaag aatttcttga ggcctatccg caatcaactt
     4681 ttgaagccat aaacaaagaa aactaccttg attttctgac aaattagtat tatcctctcc
     4741 cccgcatccg cacgcattcg gaataacaaa aaaataacga accgttgcgt tgaaccaccg
     4801 aataatagac ctactgcttc ttaatctttg agtgcatata tattgcgata tctcctacct
     4861 cttcttcccc gggtttttcc gtgatccaga ggccgagggg cttgttgatg atcaggcggg
     4921 tttgggattc tggggccggc aaaattcatt cttgcgacaa ataataaatt tccatttttt
     4981 ccgcaggagt taaaccgaat aattcttcgc ggggggtgtt gttccagagt tgattgacat
     5041 attcaaaaaa cacggtgagg gatttttcgt cgggtaaatt tattttttcg gaaatcgttt
     5101 tcacgatcgt gcccggcgaa cgactttcgt tcattgcttt ttggatatct tccacctgcc
     5161 agatatcgcc cgcgccaatc aaaaccagcg ctcgctgaaa ttcgtcttca aaatccgatc
     5221 gctggcggat ttcaagccat ttttccaaac aaaacggctc aatctcctgc gcatcgcgct
     5281 ccgccagttc ttttttaaac tccctatcca attcgctgcc cttgaatttg cgctccattt
     5341 ctttaaacct ttcttccatc ttttccgtat ccgtatcatc gtcgctttcc tcttcgctat
     5401 cccccttggc aactctctcc aacagatgct cccatctttg ctcccaattt tgtttccata
     5461 atttattctc gggatatttt tccgcataac tgatgccatt agccaaataa taataatgcc
     5521 atttcatttc atctatcgca ataccatctt ccacttgttc ggcaaacacc aaagatcgct
     5581 tcaaaatttc taaaattcct ttatccttgg tattaaccaa aacactacca ataaaatctt
     5641 ggatatcttt gtctttttcg aataatttta ttaaaaaatt tttaaccgag atttgatcta
     5701 cgaattcgcc caacacgcca acagcaaaaa tttttgcatg aaacggatca ttgtcaattc
     5761 tccgctcaat aaactcaatc gtggcaggca caaccgagcc cggatgagct ctttctatca
     5821 ataccagtgc atcagcagcc gcttcttgcg taaaatcctc atcgctttca aaaacatcca
     5881 gcaagcaata aaccgcctct ttgtcgcgca taatacccaa aataacgatc gcccaaatcg
     5941 ccgccagcga ttcgcaaatc cgaaaattct caacaatgtc gcgcaatgcc gtttttaatt
     6001 gcttgttctt gtctcgccgc gccaaataat tcaccgcttc cttaatcatc cttgggttgt
     6061 ccgaatctaa atcgttcaac ctttttttaa ttgccggatt ttcaattttt gcttcaattt
     6121 ccatggcttt gattattgaa tgtcttttat actttccaat atggaatgaa ttttaccgcc
     6181 gtggttttat gtttaatctc cgtttccaat gacaaattga ccacccacgc ttcattaggt
     6241 tcgtattttc caatgaacga cagcaatgat ttccccaaaa ccggcgactt caaatcaata
     6301 tatttaactt caaccggtag cacttcccgg ccgcgattga tcacgaaatc cacttcggcg
     6361 ccggcgatag tgcgccaaaa atttattgtc gctgccgaat ttttttcttt ttcgcgcaaa
     6421 atattcaaaa caaaattttg aaacacaaaa gaaaagcttt gcggatcgga caacaccccc
     6481 attaattcta tggaaaaatt acgcaagcca agatcatgga aatacgctac cggcgaccga
     6541 gtgatttctt tttgcttgtt ggtaaaatac ggccgaacga ttctgagcaa aaaaatcttt
     6601 tgcgcatacc acaaatattt tttcaacggc gcaaaagtca aacctatttg ctgtcccaat
     6661 tttgaataat tcaaaatttg cccggattgg ctggcgatga ttttaatcat taacgaaaac
     6721 gcttccggcc ggtcgatttc caaaagcgaa agcaagtctt tttcaacgca actgcgaaaa
     6781 atctccccca ttattttatt tttctccgaa acatgagatg ccaaaataac gcgcggataa
     6841 ccgccaaaat tgagatattc ttttaaaaaa gttttcgttc tatctttttc tatttcgaaa
     6901 taacttccaa ttctgtcttc atggcggtaa tcggttttga aatttacaaa ttccttaaaa
     6961 ttgaccggat taatttcgaa aattcttttt cgtccggcca acgattcgtg aattttttct
     7021 ttcagttcca aactgccgga tccggaaaca ataaatttat atggcaagtc caaatcatag
     7081 attcccttca aaaaaatccc ggcatccttt ttccgctgga tctcatcaat aaaaacaaaa
     7141 ctcccgggcg caagatgaat tttgatcttt tcaatcaatt ccgtctggct cgtgaaatat
     7201 ttttcatccg cctcattgtc caaacttaaa aaaatatact ttttaccgat actttcaaga
     7261 tgcgcgac
//
LOCUS       DAOQWE010000029         7144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_10871_length_7144_cov_7.597828, whole genome shotgun sequence.
ACCESSION   DAOQWE010000029 DAOQWE010000000
VERSION     DAOQWE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_7144_cov_7.597828"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKK37_01770"
     CDS             <1..786
                     /locus_tag="PKK37_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81142.1"
                     /translation="NGSCGNNGKTEIPEKINAPFLGQISIKSLSLPAITVVLGALDGF
                     NPCAMWTLLFLISLLLGMKDRKRMWILGCSFIGASALVYFLFMSAWLNLLLFVGFIVW
                     VRIIVALVAIGGGAYNLKAYFSKEPAVCANAADPKRKKVFERLKKITLQNNFYLALGG
                     IVLLAFAVNLVELICSAGLPVIFTQILSMSGLSQWQYYSYIALYILVFMADDLFVFFA
                     AMITLKMAGITTKYQKASHLIGGILMLAIGLLLIFRPEWLMLG"
     gene            886..1632
                     /locus_tag="PKK37_01775"
     CDS             886..1632
                     /locus_tag="PKK37_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81143.1"
                     /translation="MAKKAAKKGKLIVVSLGGSVVYPYGEKYNNGLDIGFLKDLRQFL
                     LGQAKKSVRFVIVVGGGKSCRIYQKAAREVLGEKKNDTDLDWIGIQATKMNALFVAKI
                     LREVAFPQVLEREPGKKEIAAFLKSNKKILMVSGWEPGWSTDYDAMQCAVFFGAKEVI
                     SASDAHFVCDKDPRKFEDARPIKELTWPEYQKLIPSKWVPGLSTPVDPVAARFAKKNN
                     LTVKKIKGSDIESFRRAVEGEDFEGSIIHD"
     gene            1686..2363
                     /locus_tag="PKK37_01780"
     CDS             1686..2363
                     /locus_tag="PKK37_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HNR81144.1"
                     /translation="MAESPQSEIPAEYKNGAVEFLGCKIDLSLRPLIPRPETEFWTRH
                     AIADLARRGRSAGRVLDLFAGSGCVGIATAKNLPAAAVDFGDIDAAAVEQIKINLKIN
                     RLSGERTRVFQTDIFENIPPGESYDAILANPPYVAAERIREVQRSVLDWEPRRALFGG
                     ADGLGIIEKFLRRADDFLRPGGIIYMEFDTFQKISIIKMLKGLSYGSFEFHKDQFQKW
                     RFIRIKK"
     gene            2336..2800
                     /locus_tag="PKK37_01785"
     CDS             2336..2800
                     /locus_tag="PKK37_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR81145.1"
                     /translation="MAFYQDQKIKKAKKVILLESDSHHIAMYRRMFQNAAFEVDLART
                     REEMLEELREIRNGEAPKPDLVLMDFVLADGYGTEVLRAIKKSHFTRDIPVFAVTNFQ
                     NPDMEREMKKSGIFPDKYMLKAHHTPAELVNEMENYLEQRSAGPDRPKTNLA"
     gene            2992..3423
                     /gene="mraZ"
                     /locus_tag="PKK37_01790"
     CDS             2992..3423
                     /gene="mraZ"
                     /locus_tag="PKK37_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNR81146.1"
                     /translation="MFIGEYTYSIDEKKRLAVPPKFRAELKKGAVITRGLDNCLFLYS
                     REEWEKLAEKLSQMPLSRADARGFARIMLSGAMEVDFDNLGRILVPDYLKDYAKLKKK
                     AVVAGLYSRVEIWDEEEWKIYKEKMEKEAGDIAERLNELGV"
     gene            3546..4445
                     /gene="rsmH"
                     /locus_tag="PKK37_01795"
     CDS             3546..4445
                     /gene="rsmH"
                     /locus_tag="PKK37_01795"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNR81147.1"
                     /translation="MVHIPVLLNETVAALNPKPGENFIDCTFGRGGHSLAILERTAPQ
                     GRILAIERDAESLSALPQELKEHPRIVFAGASYADLGEIVREKEFENIGGVLFDLGMS
                     SWHLEESGKGFAFSRDELLDMRYDRKSPLTAADILNRFPAAELRNIFQNYGQEKTAGK
                     IAAAVERERKKKEIATAGQLAKIIEREAGARRWRAAAARIFQALRIQVNCEFEHIEKG
                     IPAAFEIIAPGGRIAVITFHSGEDRIVKNIFRDFCRSGKAKTVYPKPMAPSEAEINRN
                     PRARSAKLRVLEKVLAAEERNAK"
     gene            4457..4780
                     /locus_tag="PKK37_01800"
     CDS             4457..4780
                     /locus_tag="PKK37_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81148.1"
                     /translation="MSRKTKIRVKNIVFNFKAASSFLICVLVGAAIFQLNSHIHQNSL
                     INDLEKEIVVIAEENSALEARLSQLNSMDDFDRYRAAQAENYEKIDLASVRYIQVPAG
                     GFAKR"
     gene            4796..6469
                     /locus_tag="PKK37_01805"
     CDS             4796..6469
                     /locus_tag="PKK37_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNR81149.1"
                     /translation="MIAKKAKSKTRRWRINLVLFFLLAACAGVVGRLFYLQIVLGERY
                     QVLAQGFEVFARDNQDISRGEIFFAGGQPLAINKDFFYCYASPMKINDKEQAVRTVAQ
                     ILGLEEQSLRDKFQKESLYAFIKEKLGDAEIEALERAAIEGIYVKKKKMRYYPQGEVG
                     GQLAGFVDDDGVGRYGLEDYYNDKLSAGESIFLNIDHNLQYQAQRLIAQAKEDLNAVS
                     GEAIVANPKTGAILAMAETPNFDPNDYKKVAKDGIEILKNDSCQTLFEPGSVFKAITM
                     ASALNEKKVTPETDYIDSGMVKIGGWPIYNYGHRAYGRQTMTNVLEHSVNTGVVFAQS
                     RLGDGPFVEYLEKFGIFEPTGIDLPETFSDNAEFKKGYAINYATASFGQGIWMTSIQL
                     IRAYSALANGGVLVRPSVAKIKQEETPPARQVIDPETSKTIAKMLASVVDNGFGKSAK
                     IKGYSIAGKTGTAQMSWSTLDINERGYSDKTTQSFIGFFPAEDPQFLILVKLVAPEAN
                     TAEYSSLPVFKELAQYAIYLSQIPPSEKSDDTAVPAEMETAQTQPHSGD"
     gene            6515..6586
                     /locus_tag="PKK37_01810"
     tRNA            6515..6586
                     /locus_tag="PKK37_01810"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6548..6550,aa:Glu,seq:ctc)
BASE COUNT         1861 a         1582 c         1922 g         1779 t
ORIGIN      
        1 aacggcagtt gcggaaataa cggcaaaacg gaaattcccg aaaaaataaa tgcgccgttt
       61 ttggggcaaa tttcaatcaa gagtttgtct ttgccggcga tcaccgtggt tttgggcgcg
      121 ttggacgggt ttaatccgtg cgcgatgtgg acgctgttgt ttttgatcag tctgcttttg
      181 ggaatgaagg acagaaagcg gatgtggatt ttgggatgct cttttatcgg tgcctcggcg
      241 ctggtttatt ttttgtttat gtccgcgtgg ctcaatttgt tgttgtttgt gggatttatc
      301 gtttgggtcc gcattattgt ggcgctggtg gcgatcggcg gcggcgcata caatctgaag
      361 gcttattttt ccaaagagcc cgcggtgtgc gccaacgccg ccgatcccaa aagaaaaaaa
      421 gtttttgagc ggctgaaaaa aatcactctg caaaacaatt tttatctggc gctggggggg
      481 atcgttctgc tggcgttcgc ggtcaatttg gtggagctta tctgctcggc ggggttgccg
      541 gtgattttca cccagatatt gtcaatgagc ggcttgagcc agtggcaata ttattcatat
      601 atcgcgctgt atatcttggt gtttatggcc gacgatctgt ttgtgttttt tgcggcgatg
      661 attaccttga aaatggcggg aattaccacc aaataccaaa aagcgtcgca tttgatcggc
      721 ggaattctga tgttggccat cggcctcctg ctgatattcc gccccgaatg gctgatgttg
      781 ggctgactat tgaaggtttt gggcaaatgg ggtatgatgc taatcgggta tcgttgaata
      841 atttagttta ataggctaaa tttttttggc caaaaacaca acaatatggc gaaaaaagcg
      901 gcaaaaaagg gaaaattgat cgtggtttct ctgggagggt ccgtggttta tccctatggc
      961 gaaaaatata ataacgggct ggacatcgga tttttgaagg atttgcgcca gtttttgctg
     1021 gggcaggcaa aaaaaagcgt tcgttttgtg attgtggtgg gcgggggcaa atcctgtcgg
     1081 atttatcaaa aagccgcccg cgaagtgctg ggcgaaaaaa agaacgacac cgatctggac
     1141 tggatcggca ttcaagcgac caaaatgaac gcgctgttcg tggccaaaat tttgcgcgag
     1201 gtcgctttcc cgcaggtgct ggagcgcgaa cccggcaaaa aagagatcgc cgcttttttg
     1261 aaaagcaaca agaaaatttt gatggtttcc ggatgggaac cgggatggtc cacggactat
     1321 gacgcgatgc aatgcgcggt tttttttggc gccaaagaag tgatctccgc cagcgacgcg
     1381 cattttgttt gcgacaaaga cccgcgcaaa tttgaagatg cccggccgat caaagaactc
     1441 acctggccgg aataccaaaa attgattcct tccaaatggg tgcccgggct ttccacgccg
     1501 gtggatccgg tcgcggcgcg ttttgccaaa aaaaacaatc tcacggtgaa aaagatcaag
     1561 ggcagcgaca ttgaaagttt ccgccgcgcc gtagaagggg aggattttga aggatcaatc
     1621 attcacgatt aaaaaaacgg ggaaacgccg caccgcggcg tttttctttt tttgttcaca
     1681 tcattatggc cgaatcgccg caaagcgaaa ttcccgcaga atacaaaaac ggcgccgttg
     1741 aatttttggg ttgcaaaata gacctgtcct tgcggccgct gatcccgcgt ccggaaaccg
     1801 aattttggac gcggcacgcg attgccgatc tggcgcggcg cgggcgatct gccgggcgcg
     1861 ttttggattt gttcgccggt tccgggtgcg ttggtatcgc gactgccaaa aatctgcccg
     1921 ccgccgcggt tgatttcggc gatattgacg ccgcggcggt ggaacagata aagataaatt
     1981 taaagatcaa ccgcctgtcc ggagaacgca cgcgggtttt tcagaccgat atttttgaaa
     2041 acattccgcc gggcgaatcc tatgacgcaa ttttggccaa ccctccctat gtcgccgcgg
     2101 agcgcatccg agaagtgcaa agatcggtgc ttgattggga gccccgccgc gcgcttttcg
     2161 gcggcgccga cggattgggg attattgaaa aatttttgcg ccgcgcggat gattttttgc
     2221 gtcccggagg aataatatat atggaatttg acacatttca aaaaatcagt ataataaaaa
     2281 tgctaaaagg tcttagctac ggatcgtttg aatttcataa agaccaattt caaaaatggc
     2341 gttttatcag gatcaaaaaa taaaaaaggc caaaaaagtg attttgctgg aaagcgattc
     2401 gcaccacatt gcgatgtacc gccggatgtt tcagaatgcg gcgtttgagg ttgacttggc
     2461 gcgcactcgc gaagaaatgc tggaagaatt gcgcgaaatc cggaacggcg aggcgcccaa
     2521 gcccgatttg gtgttgatgg attttgttct ggccgacggt tacggcacgg aagttttgcg
     2581 cgccattaaa aaaagccatt tcacccgcga cattccggtg tttgcggtga cgaatttcca
     2641 aaatcccgat atggaaagag aaatgaagaa atcgggaatc tttccggaca aatatatgct
     2701 gaaggcccat cacactcccg ccgaactggt gaatgaaatg gaaaactatc tggaacagag
     2761 gtcggccgga ccggatcggc caaaaacgaa tttggcctag gactttttgc cggatttcca
     2821 ccgcccgccc cggggcggtt tttggttaga aaaaattggg cggcctgtgg ataaccggcc
     2881 gtttttagct ttattttgct taatttgaca ttgggaatct atgggtttaa agtatgtata
     2941 tgtgggagat tgtgttgatt ggtggaaaaa tgtggataac ctcgtattat tatgttcatc
     3001 ggcgaatata cctattccat tgacgaaaag aagcgcttgg ccgtgccgcc caaatttcgc
     3061 gccgaattga agaagggggc ggtgatcacc cgcgggctgg ataattgtct gtttttgtat
     3121 tcccgcgaag aatgggaaaa gctggcggaa aaattgagcc agatgccgtt gtcccgcgcc
     3181 gatgcgcggg gattcgcgcg gattatgctg tcgggcgcca tggaagtgga ttttgacaat
     3241 ctgggaagga ttttggtgcc ggattatttg aaagactatg ccaaattgaa gaaaaaagcg
     3301 gtggtggccg gtttatacag ccgtgtggaa atttgggatg aagaagagtg gaaaatctac
     3361 aaagaaaaaa tggaaaaaga agccggcgat atcgccgaga gattaaacga gctgggagtc
     3421 tgacaaatta aaaattgaaa aatcaaaaat caaaatgaca atctaaaatt gaaaattaca
     3481 taatcaaaat tccgcgagga attttacctt aatttttcat tttgatattt taaattttaa
     3541 ttataatggt gcatattccg gttttgctaa atgagacggt cgcggcgctg aaccccaagc
     3601 ccggcgaaaa tttcattgat tgcacttttg gacggggcgg gcacagtttg gcgattctgg
     3661 aaagaaccgc gccgcaggga cggattctgg cgattgagcg cgacgcggaa agcctgagcg
     3721 ctctgccgca agaattgaag gagcatccgc ggattgtttt tgccggtgct tcctatgcgg
     3781 atcttggcga aattgtccgg gaaaaagaat ttgaaaatat tggcggcgtt ttgtttgatc
     3841 tggggatgtc atcgtggcat ttggaggaaa gcggcaaagg attcgcgttt tcccgggacg
     3901 aattgctgga tatgagatat gaccggaaat cgccgcttac cgccgccgac attcttaacc
     3961 gttttcccgc cgccgaattg cgaaacattt tccaaaatta cggacaagag aaaacggccg
     4021 gaaaaatcgc cgcggcggtg gaaagggaaa gaaaaaagaa agagattgcc actgccggac
     4081 agctggccaa aatcattgaa agggaagcgg gcgcccgccg atggcgcgcc gccgccgcca
     4141 gaatttttca ggcgctgaga atccaagtta attgcgaatt tgaacacatt gaaaaaggga
     4201 ttcccgctgc gtttgaaatt atcgctcccg gcggccgaat cgcggtgata acttttcatt
     4261 ccggagaaga ccggattgtc aaaaacattt tcagggactt ttgccgatcg ggcaaggcaa
     4321 aaaccgttta tcccaagccg atggcgcctt ccgaagccga aatcaaccgg aatccgcgcg
     4381 cccgttcggc aaaattgcgg gtgctggaaa aagtcttggc cgcggaagaa cgtaatgcca
     4441 aatgataatc aacattatga gcagaaaaac aaaaatccgg gtaaaaaaca ttgttttcaa
     4501 ttttaaggcc gcttcttctt ttttgatttg cgttctggtg ggcgccgcga tttttcagct
     4561 taattcccat atccaccaaa attcattgat caacgacttg gaaaaggaga ttgttgtcat
     4621 cgccgaagaa aacagtgctt tggaggcccg cctttcacag ttgaactcaa tggacgattt
     4681 tgaccggtac cgcgcggcgc aggcggaaaa ctacgaaaag attgatcttg cttcggtgcg
     4741 ctacattcaa gtcccggcgg gaggattcgc caaaaggtaa aaatcgcatt ctgccatgat
     4801 tgccaaaaaa gcgaaaagca aaacccgccg ctggcggatc aatctggtgc tgtttttttt
     4861 actggcggca tgcgccggcg tggttgggcg cttgttttat ttgcagatcg ttctcgggga
     4921 gcgttatcaa gttctggcgc agggatttga agttttcgcc cgcgacaatc aggatatttc
     4981 gcgcggcgaa attttttttg ccggcggcca gccgctggcc atcaacaaag attttttcta
     5041 ttgctatgcg tcgccgatga aaatcaatga caaggagcaa gcggtgcgca ccgttgcaca
     5101 aattcttggc ctggaagagc agtctttgcg ggataagttt caaaaagaat ccctttacgc
     5161 ctttatcaag gaaaaactcg gtgatgccga aattgaagcg ctggaaaggg cggcaattga
     5221 aggtatttac gtcaaaaaga aaaaaatgcg ctattatccg caaggcgagg tgggcggcca
     5281 gctggcggga tttgttgacg acgacggtgt cggtcgctac ggactggaag attattacaa
     5341 cgacaaatta tccgccggag aaagcatttt tctcaacatt gaccataatc ttcaatacca
     5401 ggctcagcgc ctgatcgcgc aggccaaaga agatcttaac gcggtttcgg gcgaagcgat
     5461 tgtggccaat ccgaaaaccg gcgcgattct ggcaatggcc gaaactccca attttgatcc
     5521 caacgactat aaaaaggtcg ccaaagacgg gattgagatt ctgaaaaacg attcctgcca
     5581 aaccttgttt gagcccggct cggttttcaa ggctatcacg atggcgtcgg cgcttaatga
     5641 aaaaaaagtg acgccggaaa cggactatat tgattcggga atggtgaaga tcggcggctg
     5701 gccgatttac aattatgggc atcgtgccta tggccggcag acgatgacaa atgttttgga
     5761 acattcggtg aacaccggcg tagtcttcgc ccaaagccgg ctgggggatg ggccgtttgt
     5821 tgaatatctt gaaaaattcg gcatttttga gccaacggga atcgatttac ctgaaacctt
     5881 ttcggacaat gccgagttca aaaaaggcta cgcgatcaat tatgcgacgg cgtcgttcgg
     5941 gcaggggatt tggatgacgt caattcagct gatccgcgcc tattcggcgc tggccaacgg
     6001 cggcgtcttg gtccgtcctt cggtggcaaa aatcaaacaa gaggaaacgc cgccggcgcg
     6061 gcaagtgatt gatccggaaa cttcaaaaac cattgccaaa atgctggcca gcgtggtgga
     6121 caacggtttt ggcaaatcgg ccaaaatcaa aggatattcc atcgccggca aaaccggcac
     6181 ggcgcaaatg tcatggtcca cgcttgatat caacgaaagg ggatattccg ataaaaccac
     6241 tcaaagtttc atcggctttt tcccggccga agatccgcaa ttcttgattc tggttaaatt
     6301 ggtcgctccc gaagccaata ccgccgaata ttcgtccctg cccgtgttca aagagctggc
     6361 gcaatacgcg atctaccttt cccagattcc tccttccgaa aaatccgacg atactgccgt
     6421 tcccgctgaa atggagacgg cacaaaccca accgcattcc ggcgattgaa aaaaaaccgc
     6481 gtttgggcta caatgtcgct gttcggcggt ttttggcccc atcgtctagt ggtcaggaca
     6541 cttggttctc agccaaggaa caccggttcg actccggttg gggccgcctt tcacctcgcc
     6601 atagcccggc ttgattggca gtttatggtt gatggaaaaa gttgatggaa aaatccgaat
     6661 gccggaagcg attaccgtag tattataatc ttccaatccc gctagcgcgg gattttgcgt
     6721 ttgccatcct tgcttgcgtt tttgaaattg gctaaaggta ggggataaca acaggggggc
     6781 taaccactca ctggatctaa aattaaaggg ggtctatccc tcgttactgc gagggttaga
     6841 ccccctttaa ttttgtgcct acaattcttt gccatggtct aacctccgtg attatccctc
     6901 ggattacctc gggacaagcg cgggggatag acccttaaaa tacaagaata cgttacccca
     6961 gtgagtggtt acggcggcgg gcgatagatt caaactttgg aatatggtat actcaacgag
     7021 ttcggcggtt tggtttaata atagacctac tgcgtaaata tttcctactg caatttccat
     7081 atttcggcga cgccgaattc gtcaatcgtc ggcatattta agccaatatg cctcctcact
     7141 cctc
//
LOCUS       DAOQWE010000030         7117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_10920_length_7117_cov_4.303314, whole genome shotgun sequence.
ACCESSION   DAOQWE010000030 DAOQWE010000000
VERSION     DAOQWE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10920_length_7117_cov_4.303314"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKK37_01815"
     CDS             <1..896
                     /locus_tag="PKK37_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNR81150.1"
                     /translation="QTDIKINGDDGPVSAKPNTLITLSWISNNTNNCTASGNWSGSKA
                     LSGYESVVMSSGSMVFSLSCTGDQGTVSDSITVNAAAFGIGLAESIEISSGATATLSA
                     EITGTAPSSPVYQWSCTGGSLSDSSILSPVYTAPAVTSDKRYVCTLTVRDGNAAAVSK
                     KINVNVKPVPQNVVELEKQIAEIKALIESLQKQLAALAGLPATGAGSAKYSCAQLTKY
                     LVYNTQNDPEVKCLQEFLIAQGFSGVTATGNYQTATRDAVKAFQEKYAAEILAPYGLT
                     YGSGNVGNDTRRKINALITAK"
     gene            928..1962
                     /locus_tag="PKK37_01820"
     CDS             928..1962
                     /locus_tag="PKK37_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81151.1"
                     /translation="MSHDFLKFSAIGVSALAATLTLCFAILAWQGPTAAPPADNVDAP
                     INTGPNSQIKAGDLGFKNGANDPYYIHMDGASFSLKNNSGSERFIVDQDGHVGIGTTS
                     PAKALEVVGDTKSGAFCLDDQCCSTWEECANFVGAGGISGKVQNPIYSGVCAWDQTAV
                     ISSGCCPKNTCALMISGGVCYPDGWIMALPALGGAGWLQEDYGGRLSSLPEGNVVVCR
                     AGYWRAAPYYTTVGSVYDCYPNQGILGNELACDKGTCNGYKCVYKTCNRDSDCAGAIA
                     AGSKCDKGICCVNGSCNVGGRCLTGIYVHGVQLEKCVSGVMQPMNCVFTGLCPDGFSC
                     NPATSLCEHP"
     gene            2204..3334
                     /locus_tag="PKK37_01825"
     CDS             2204..3334
                     /locus_tag="PKK37_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR81152.1"
                     /translation="MINKIISKIKPKALDNFIAKLGFFKSKAADIAKHYISGLMNGFK
                     RFNLQALHLATEEGSYSYRQLRYFIGDADWDENQLNEKRIQFLESDRRTKTKKNGCIS
                     FDDTSVVKSGTHTDGVAKQYAPSVSQIANCKVVVTAHYSDDVKDFPLDAESYYHGEES
                     KLDLVCSLMDKAVERGLRFKWFNFDSWYCTKQVIEKTEALKKYFVSHLKSNRVVIWKN
                     KRTSVSDLVKIISAENQKDAVIDLGKIYIKSLGNYRLIVKNATCFVANNFNETPEAII
                     AAYSMRWHIDEFYHRAKDDLSFDQFQTRNGLSIMRHWMMVFLAYTFWTHCKLKSVFSK
                     IYQGAINSLADFAKLMQNLNIVRLAKESTNVILVKFKLNFLN"
     gene            3385..3525
                     /locus_tag="PKK37_01830"
     CDS             3385..3525
                     /locus_tag="PKK37_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81153.1"
                     /translation="MYLKNKMIVAALCALIAGIGIFGAPVNTTSAAVLSLDQIEQMIA
                     NN"
     gene            3538..4431
                     /locus_tag="PKK37_01835"
     CDS             3538..4431
                     /locus_tag="PKK37_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81154.1"
                     /translation="MFKPSAIGISALAATLTLCFTILAWQSPTAAPPADNVDAPINTG
                     PNSQIKAGDLGFKNGANDPYYIHMDGASFSLKNNSGSERFIIDQDGHTGIGTASPAKT
                     LEVAGDTKSGTFCLPESTGGPEKCCATWAACFGQCSCGEWSDGACGDGTVCSVDQRRQ
                     TRTCSPAGCDSEGRCVADAACGGSCTPDCDDRECGDDGCGGECGTCSGDLVCSDGECY
                     DCNQLCADQEATCGWLIDGAGGHFCGDRYCGDCPSEGCHVNTCRPYCSDLDCLSILSG
                     GSLNMCVIAAGGTFSDTILCI"
     gene            4462..6981
                     /locus_tag="PKK37_01840"
     CDS             4462..6981
                     /locus_tag="PKK37_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81155.1"
                     /translation="MKIANQINLFLAAIFLVAVFLGGSIFAYQSNLSSCTSDWQCSSG
                     NCSNGVCCDSGACGWGDGTINVRECVAAGQIRENATASAICRSGVWKINLRGWGCSTT
                     AEGDGKGQYYQLPVSQGFACDQGTCQLVGDKYFCRDCAIDPGVAGTCAAGETCPSGTV
                     CNNKVCCPSGYCGWGDGVGFNRQCVASGEYRENALNRAVCRSGTWKTVASGWGCSAGF
                     PCDSGTCQNNYCAAAFPFNAPGEMLETASRCSGTCKSCSAQGKQCGIVSDQCGGWLFC
                     GTCASGQTCNASGQCVSSCVPATCSSLSKLCGVWNDNCGGAVNCGSCLANESCIASGQ
                     CVANTDCTPNFSKKCDSGHLYWYDSCGNRGGVAQNCGANSATQNFQCRGNWVQQEVVA
                     KDCVSGACTENKTWSDSVNCELSGKICVNGACADNDTTPPSLSDLSPSGTVNGANVTL
                     AVSTNEPSECRYGDKDTAFGLMALKFGTANRINHSALIVLPSPGNYNYFVRCADTAGN
                     AVSASAKISFAYANPAASSAVPAASPGSSSPTGAPVALSTPADKTPPSISDPLPAGEI
                     SGDEAEISVVTDEKSLCRYDISDKEYGAMANEMESDSAGTFHKKKIAPAEAGSHLYYA
                     KCKDGAENISRQSAMIKFVVVKNKPGPIISGPFPSGTIYQEEIELAFSTNVPSVCRYS
                     KDNRGFEEMSDVFSSVGGLDQSADVVLDGYGKYDYHIRCQDENGNALGSYALVSFEYK
                     DPLATEKEQECSTMDVGTKDDACNNAADCICDPDCPAEGEGADPDCANVAVQEKGGNP
                     MVFLIAGLSALLLAVIAAIVIVARRRKKSALMSDADKLDLE"
BASE COUNT         1719 a         1652 c         1958 g         1788 t
ORIGIN      
        1 cgcaaacgga cattaaaatc aacggcgatg acggcccggt atcggccaag ccgaatacct
       61 tgatcacgtt atcgtggatt tccaacaata ccaataattg cacggcgtcc gggaattggt
      121 ccggcagtaa agctctttcc ggatacgaaa gcgtcgttat gtcatccggg agtatggttt
      181 tttccctttc ctgcacgggc gatcagggaa ccgtaagcga cagcatcacg gtgaacgcgg
      241 cggcgttcgg gatcggtttg gcggagtcaa ttgagatttc ttccggcgcg actgccactt
      301 tgagcgcgga aattacgggc acggcgccct cttccccggt ttatcaatgg agctgtaccg
      361 gcggcagttt gtcggacagt tcaatcttaa gcccggttta taccgcgccc gccgtgactt
      421 cggacaagcg gtatgtttgc actctcacgg ttcgcgacgg caacgccgcc gccgtttcca
      481 aaaagattaa cgttaacgtc aagccggtgc cgcagaatgt cgttgaattg gaaaaacaaa
      541 ttgccgagat caaggcgttg attgaatctt tgcaaaaaca gctggcggca ttggccggct
      601 tgccggctac cggggcggga agcgcaaaat attcttgcgc ccagttgacg aaatatctcg
      661 tctacaacac gcaaaatgat ccggaggtca aatgtttgca ggaatttttg atcgcgcaag
      721 gcttttccgg cgtgacggca accggcaatt atcaaacggc gacccgcgac gcggttaagg
      781 ctttccagga aaaatacgcc gccgagattt tggcgcccta tggcttaacc tatggttcgg
      841 gcaatgtggg caacgatacc cgccggaaga tcaacgcgct gattactgca aagtaatcca
      901 ttaatgctta atactaactt taaatttatg tctcatgatt ttttaaaatt ttcagcgatt
      961 ggcgtcagcg ctttagcggc aactttgacc ttgtgttttg ccattctggc atggcaaggt
     1021 ccgaccgcgg cgccgccggc cgacaatgtg gacgcgccaa tcaacaccgg tccaaattcg
     1081 caaatcaaag ccggcgatct gggattcaaa aacggcgcca acgatccgta ttatatccac
     1141 atggatggcg ccagtttttc gttgaagaac aatagcggca gtgagcggtt tatcgtggac
     1201 caggacggcc atgtgggcat cggcacgaca tcgccggcaa aagcgctgga agtggttggc
     1261 gacaccaagt cgggcgcctt ttgcttggat gaccaatgct gttccacttg ggaagaatgc
     1321 gcgaatttcg tgggcgcggg cggaatttca ggtaaagtac aaaatccgat atacagcggc
     1381 gtatgcgcct gggatcaaac cgctgttata agctccggtt gttgtccaaa aaatacctgt
     1441 gctttgatga tatcaggcgg ggtttgctat cccgatggtt ggattatggc gttgcctgct
     1501 ttgggcggtg ccggttggct tcaggaagat tatggcggca ggctctcctc cttaccggag
     1561 ggaaacgtgg ttgtttgccg cgccggttat tggcgcgcgg cgccgtatta taccacggtt
     1621 ggatctgtgt atgactgcta ccccaatcaa ggtatacttg gtaatgagct tgcttgtgac
     1681 aaagggactt gcaatggata caaatgcgtc tacaaaactt gcaatcgcga cagcgattgc
     1741 gccggtgcca tcgccgcggg cagcaaatgc gataaaggaa tttgttgcgt taatggcagt
     1801 tgcaatgtgg gcggccgatg tttgaccggt atctatgtac acggtgtcca attggaaaaa
     1861 tgcgttagcg gggtgatgca accaatgaat tgtgttttta ccggactttg tccggatggt
     1921 tttagttgca atccggcaac ttcgctttgc gagcatccgt gagcggtcat ttgtccggat
     1981 ttgatttgtt tttgattatt ggcggtaaaa aggtctaaat tgtcagtgtt taatgaagtg
     2041 aaaaaatatt gcgaataaat cagtgagatg cctcgtcgcg ggagaacggg tatcccgcca
     2101 gttaaatcaa tactttgaca aaaatgacta taattttaag ctgaatgggg gtaaaatgaa
     2161 gctaaaatac gttaccagcc ccatttaacc taattttact tccatgatca acaaaattat
     2221 ttcaaaaatc aagcccaaag cgctggataa ttttattgcc aaactgggat ttttcaaaag
     2281 caaggccgcg gatatcgcca agcattacat ttcgggattg atgaatggtt ttaaaagatt
     2341 taaccttcaa gcgttgcatc tggccaccga agaaggatcg tattcctacc gccaattgcg
     2401 atattttatc ggcgatgcgg actgggacga aaaccagcta aatgaaaaac gaattcaatt
     2461 cctggaaagc gacaggcgca ccaagaccaa gaaaaacgga tgcatctctt ttgacgacac
     2521 ctcggtggtg aaatccggaa cccataccga tggcgtcgcc aagcaatacg cgcccagcgt
     2581 tagccaaatt gccaattgca aggtggtggt taccgcgcat tattccgacg atgtgaaaga
     2641 ttttcccttg gatgcggaaa gctattatca cggcgaagaa tcaaagcttg atttggtttg
     2701 ttcgttgatg gacaaggccg ttgagcgcgg tttgcgcttt aaatggttta attttgacag
     2761 ttggtattgc accaagcaag tgattgaaaa aactgaagcg ttaaaaaaat atttcgtttc
     2821 gcatttgaaa tcaaaccgtg tcgtcatctg gaaaaacaag agaacttcgg taagcgattt
     2881 agttaaaatc atttctgccg aaaatcaaaa agatgcggtt atcgatcttg gaaaaatcta
     2941 tatcaaaagt ttgggaaatt atcgcttgat tgttaagaat gcgacctgct ttgtcgccaa
     3001 taattttaat gaaacgccgg aagcgataat tgcggcatat tcaatgcgct ggcatattga
     3061 cgaattctat catcgcgcca aagatgattt atcttttgac caattccaaa cccgaaacgg
     3121 cttatcgatc atgcggcact ggatgatggt atttttagct tatacttttt ggacgcattg
     3181 caaactgaaa agcgtatttt ccaaaatcta tcagggcgcg atcaacagcc tggcggattt
     3241 cgctaaactg atgcaaaatt tgaacatcgt tcgcttggca aaagaatcaa ctaacgtgat
     3301 tttggtgaaa tttaagctga attttctcaa ttaagagcta atttggccca aattttgtca
     3361 aagtattgtt aaataaaaaa atatatgtat ttaaaaaaca aaatgatcgt ggcggcgctg
     3421 tgcgcgttga tcgcgggaat cggaattttt ggtgcgccgg tgaataccac cagcgcggca
     3481 gttttgagct tggatcagat tgaacaaatg atagcaaaca actaaatttt aattcatatg
     3541 tttaaacctt cagcgattgg catcagcgct ttagcggcaa ctttgacctt gtgttttacc
     3601 attctggcat ggcaaagtcc gaccgcggcg ccgccggccg acaatgtgga cgcgccaatc
     3661 aacaccggtc caaattcgca aatcaaagcc ggcgatctgg gattcaaaaa cggcgccaac
     3721 gatccgtatt atatccacat ggatggcgcc agtttttcgt taaagaacaa tagcggcagt
     3781 gagcggttta ttatagatca ggacggccat acgggcatcg gcacggcatc gccggcaaaa
     3841 acgctggaag tggccggcga taccaaatcc ggcacctttt gcttgccgga atcaacgggc
     3901 ggaccggaaa aatgctgtgc cacttgggcg gcgtgttttg gccagtgttc ttgcggcgaa
     3961 tggagcgatg gcgcgtgcgg cgatggcact gtttgctctg ttgaccagcg ccgccaaacc
     4021 cgaacttgtt ctccggccgg atgcgattcc gaaggcagat gcgttgcgga tgccgcctgc
     4081 ggcggttcgt gcacgccgga ttgcgatgac agagaatgcg gtgacgacgg ttgcggcggc
     4141 gaatgcggca cctgttccgg cgatttggtg tgcagtgatg gggaatgcta tgattgcaac
     4201 cagctttgcg ccgatcaaga agcgacttgc ggctggttaa tagacggtgc gggtggacac
     4261 ttctgcggtg accgttactg tggcgattgt ccatcggagg gatgtcatgt aaacacttgt
     4321 cgtccatatt gcagtgatct tgattgttta agtattttgt ccggcggctc gttgaatatg
     4381 tgcgtcattg cggccggagg aactttctcg gatacgattc tctgcattta atcaaatcaa
     4441 gggccctaaa tgcggctaac aatgaaaata gcgaatcaaa taaatttatt tctggcggcg
     4501 atatttctgg tggcagtgtt tcttggcggc agtatttttg cctatcaaag taatttgtct
     4561 tcttgtacca gtgattggca atgcagttcg ggaaattgca gtaacggagt atgttgcgat
     4621 tccggcgcct gcggctgggg cgacggcacg ataaatgtcc gcgaatgcgt tgccgccggc
     4681 cagatccggg aaaatgccac ggcgagcgcg atttgccgca gtggtgtttg gaagatcaat
     4741 ctccgcggct ggggttgttc aaccaccgcc gaaggagacg gcaagggcca gtattaccaa
     4801 ctgccggtca gccagggttt tgcttgcgac cagggaacgt gccagcttgt cggcgacaaa
     4861 tatttttgcc gtgattgcgc gattgatccg ggcgtggcgg gaacctgtgc cgccggcgaa
     4921 acttgtccgt cgggcacggt ttgcaacaac aaagtctgct gtccctcggg atattgcggt
     4981 tggggcgacg gtgtcggatt caaccgtcaa tgcgttgcca gcggagagta tcgggaaaat
     5041 gccttgaatc gggcagtttg ccgctccggg acgtggaaaa cggtggcgtc cggctggggt
     5101 tgttctgccg gatttccctg cgattcggga acctgccaaa acaattattg cgccgcggct
     5161 tttccgttca acgctcccgg cgagatgctg gaaaccgcct cccggtgttc gggaacctgc
     5221 aaaagctgtt cggcgcaggg aaaacagtgc ggcattgttt ccgaccaatg tggcggctgg
     5281 cttttttgcg gcacctgcgc ttcggggcag acttgcaatg cctccggcca gtgtgtttct
     5341 tcgtgcgtgc cggcaacatg ttcgtcgctg agtaaattgt gcggtgtctg gaacgacaat
     5401 tgcggcggtg cggtaaattg cggttcttgc ttggccaacg aatcctgcat cgcctccggc
     5461 cagtgcgtcg ccaatactga ttgcacgccg aatttttcca aaaaatgcga cagcggccat
     5521 ttgtattggt atgattcttg cggcaaccgg ggaggagtgg cgcaaaattg cggtgccaat
     5581 tcggcgacac agaatttcca gtgccgcggc aactgggtcc agcaagaagt ggttgccaaa
     5641 gattgtgtaa gcggcgcgtg cacggaaaac aaaacatgga gcgacagcgt caattgtgaa
     5701 ttgtcgggaa aaatttgcgt caacggcgcg tgcgccgaca atgatacgac tccgccgtcg
     5761 ctttccgatt tgtctccttc cggaacggtt aacggcgcga atgtaacgct tgccgtttcc
     5821 accaacgagc cctccgaatg ccgctatggc gacaaagata cggccttcgg tctgatggct
     5881 ttgaaatttg gcaccgccaa ccggatcaat cattccgcgc tgatagtcct gccctctccg
     5941 ggaaactaca attattttgt ccggtgcgcc gataccgccg gcaacgctgt ttccgcttcg
     6001 gccaaaatat cgtttgctta tgccaatccg gcggcgtctt ccgcggtgcc ggcggcaagc
     6061 cccggctcgt cttcgccaac cggcgctccc gtcgcgcttt ccacgccggc ggacaagact
     6121 ccgccgtcaa tttccgatcc tttgccagcg ggagagattt ccggcgatga agcggagatt
     6181 tccgtggtta ccgacgaaaa atcgttgtgc cggtatgata tttcggacaa ggaatacggc
     6241 gcgatggcaa acgaaatgga aagcgactct gcgggaactt ttcacaagaa aaaaatcgcg
     6301 ccggcggaag cgggcagtca tctttattac gcaaaatgca aggacggcgc ggaaaatatt
     6361 tcccggcagt cggcaatgat taaatttgtt gttgtcaaaa acaaacccgg accgatcatt
     6421 tccggccctt ttccctcggg aacgatttac caagaagaaa ttgagctggc attttccacg
     6481 aatgttccgt cggtgtgccg ttattccaaa gacaatcgcg gatttgaaga gatgtcggac
     6541 gttttttcca gtgtcggcgg attggaccag tcggcggacg tcgttttgga cggttacggc
     6601 aagtatgatt atcacatccg ctgccaggat gaaaacggca atgctttggg gagttacgct
     6661 ttggtttcgt ttgaatataa agatcctttg gctaccgaaa aagagcaaga atgttcaacg
     6721 atggacgttg gcaccaagga cgatgcgtgc aataacgccg cggactgcat ttgcgatccg
     6781 gattgcccgg cggaaggcga aggtgcggat ccggattgcg cgaatgttgc cgttcaggaa
     6841 aagggcggca atccgatggt gtttttgata gccggccttt ccgcgcttct tttggcggtg
     6901 attgccgcga ttgtcattgt ggcgcggaga aggaagaagt ccgcgttgat gtctgatgcg
     6961 gataaactgg atttggagta atttgaagaa tgaattacat tgttgcgcat ttcaagagat
     7021 ttgacggaat tgtttttttc gctcctctgg cgctggcggg actggggttg ctttccattt
     7081 acagcaattt gtcgcgcgcg ggcgactttt ccgcttt
//
LOCUS       DAOQWE010000031         7103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_10953_length_7103_cov_8.286322, whole genome shotgun sequence.
ACCESSION   DAOQWE010000031 DAOQWE010000000
VERSION     DAOQWE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10953_length_7103_cov_8.286322"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..617)
                     /locus_tag="PKK37_01845"
     CDS             complement(102..617)
                     /locus_tag="PKK37_01845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR81156.1"
                     /translation="MLRLVKPTKKYEKSWREAVEEFRKDSKTIKLWEVLGNPDDLDGI
                     IRNARLHSQDKNLPKGWVPYDLLWLVDGDEIVGIASIRHKLNSFLLKVGGHIGYEVKP
                     SKRGCGYANKILQLSLKRFEKLGIEKILVTAFDNNVASWKAIEKNGGKLKNRVKVENE
                     KDTTRRYWIKL"
     gene            complement(611..1114)
                     /locus_tag="PKK37_01850"
     CDS             complement(611..1114)
                     /locus_tag="PKK37_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNR81157.1"
                     /translation="MEIKAVKGDITKLEVEAIVNATDEKMSGGGGVDLAIHRAAGPEL
                     LEECKKFGGCKTGEAKITKGYNLPAKYVIHTAGPVYGKEEDDKAFKLLANCYKNCLGL
                     AQDNNIRTIAFPAISTGAYGYPKNEAAKIALAAVKEFCAVNSDSFDQIIFVCFDNENY
                     EIYKKLC"
     gene            complement(1121..2242)
                     /locus_tag="PKK37_01855"
     CDS             complement(1121..2242)
                     /locus_tag="PKK37_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81158.1"
                     /translation="MIKREIQEKIEKELFQGKIIIIYGARRVGKTTLAKEILAKYKDQ
                     GSYFNCEVLSVQEALAKPEPKMIKDYLGNAKLIVLDEAQRIKNIGLILKVFIDAYPET
                     QILATGSSSFDLANKINEPLTGRKIVFDLYPFSLSEIKNGGVARINARLEKILRYGVY
                     PEVFIAPEDVAFKRLNEIASSYLFKDVLNFEGIKKSSVVAKLLQLLALQLGNEVSFSE
                     LANRLNINRLTVEKYVNILEQCFIIFRLNSFGRNLRHEITKSVKIYFYDLGIRNNLIN
                     NFNPLEIRNDAGFLWENFCVLERMKRNHYRDVFSNNYFWRTYDQKEIDYIEEREGKLF
                     AYEFKFSKEAIKAPVKFLEAYPNSEFKTINRDNYPEFLL"
     gene            complement(2341..2790)
                     /locus_tag="PKK37_01860"
     CDS             complement(2341..2790)
                     /locus_tag="PKK37_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNR81159.1"
                     /translation="MAKQFLHEKSNVEGDVDLGENVSVWAFASIRGDEGKIIIGDNSN
                     VQDGAIIHGAVSIGKNVTIGHNAVIHGAKIGNNVLIGISSVILDGVEIGDWSIVAAGS
                     VVVPNSKIEGGSVVMGLPGKVARLVNENDKKYIQSACQNYLDKIKDY"
     gene            complement(2811..3194)
                     /locus_tag="PKK37_01865"
     CDS             complement(2811..3194)
                     /locus_tag="PKK37_01865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR81160.1"
                     /translation="MAKTKVCLIVENKKKELLLQLRDEDPEIGKWVLFGGGVKDGETP
                     ENALAREIKEELGFEIGCAGFFAEYIDRGIKQMIYILAEPVELENLNLGEGAAMRFFA
                     PAELQNLNIGFNFRQIINDYLQWQN"
     gene            complement(3235..3393)
                     /locus_tag="PKK37_01870"
     CDS             complement(3235..3393)
                     /locus_tag="PKK37_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81161.1"
                     /translation="MLIYDHGSRKEEEQKTRPAEMFAALPEIIEKLKEKFKFVRLDEM
                     KFDSNETL"
     gene            complement(3470..3637)
                     /locus_tag="PKK37_01875"
     CDS             complement(3470..3637)
                     /locus_tag="PKK37_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81162.1"
                     /translation="MENKNGEIFFDDCPICRAMKAAQEQGRDLSLEELKAAFQEAKNE
                     GGVVGGEWFEK"
     gene            complement(3911..6076)
                     /locus_tag="PKK37_01880"
     CDS             complement(3911..6076)
                     /locus_tag="PKK37_01880"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNR81163.1"
                     /translation="MMEFIFLLLFLLVALHLKNQIEGLREEIDSLRARFDAARRKTDQ
                     VAITEMPAQIEKETPVALSDSSSPLPVSKKEDSGSFEFKLGSKFFTGVGVVAIICAAG
                     FFLRYAFENDLINEWGRVALGAIAGAILLAVGELTRKKYPNYGQALTGGGLGVLYVSF
                     YAAFSFYQLIVQPAAFLSMIGVTAAGILLSLRQNSMSLAIFAQVGGFLTPLLIDNGAG
                     SPHIPFLYIALLNLAIFLTAFCKLWAPLSLTGIIGTALAYGYWYLNFYTADQFFPALA
                     YASLFFAIFLAISFIQHYVKKAGENAWDLLVVILAPLLYFHAAYPVIDSLYPQLSGPF
                     VAGLGILYCLLAVMAGRARLQSLLFRHFLMSAGFVLLSIAVPIQFDGEWIVVGWAAEA
                     LVFISSGFKMNFGAYRLMGNGLLMLTLFKTLFFMESLPLTARPIVNDRFFAYAAFFIA
                     AAGAAYLYRRYRQSANENEKSVFSILTLSAAGMFLFGGSLEIGDFFAGYWYPIFWSAA
                     GLLFAWLSFKVSAGILRPVAYFAFGIAFFRLLFSETSVVLADHNPIFNFRVMAFVVSA
                     LLGRVFLSMLIGNKEKISADEYRLFRPALFAYFHILVLWIVSAEIVSACDLAKIGAAK
                     TALREIENLKNVLLSAAWMVYGAVLMVWGIAKKAIYERFSAICLFIIVIAKVFLVDTA
                     NLGDLYRFFSFIIMGCVLLFTGYLYYRFQDRIREFIRGK"
     gene            complement(6155..6814)
                     /locus_tag="PKK37_01885"
     CDS             complement(6155..6814)
                     /locus_tag="PKK37_01885"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR81164.1"
                     /translation="MARTILSPNQTRLLEIIANDKEICHRFYLTGGTALAEFYLHHRL
                     SEDLDFFSENEFDVQAVSVFFKKNKKIIGFGKIDYQQSFNRNLFFVDFAGDIVKAEFT
                     YFPFTEIQPKTEIGDLRIDGALDIAVNKIFTIYQNPRARDFIDVYCLHGEFGFAVGDL
                     AKKARIKFDTHIDLLQLGSQFLRAVEVKDYPIMKKKIVAKDWQNYFVGEAKKLKDNII
                     K"
     gene            complement(6835..7035)
                     /locus_tag="PKK37_01890"
     CDS             complement(6835..7035)
                     /locus_tag="PKK37_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81165.1"
                     /translation="MKNWSIDIKELKKDKGKYAVWRLEQMVNFGLDGQKLDGRELKKY
                     WPKLRIDPRKKKYLAMLLGIRE"
BASE COUNT         2134 a         1770 c         1211 g         1988 t
ORIGIN      
        1 gcaaaaccgc ggcgcgtgcc tccccttccg gcgcaaacag caatccgtcc agccgtaatt
       61 catctttggt attaacccgg caaaatgtag ttttcatgat tttataattt gatccaatat
      121 ctcctggtgg tatctttttc gttttcaacc ttaaccctgt tttttaattt tcctccgttt
      181 ttttcaatgg ctttccatga agcgacatta ttatcaaaag cggtaaccaa tattttttca
      241 atccctaact tttcaaacct ttttaacgac aattgcaata ttttattggc atatccgcac
      301 cccctctttg acggcttgac ttcataaccg atatgtccgc ccacttttaa caagaacgag
      361 tttaatttgt gccggatgct cgcaattcca acaatttcat cgccgtcaac cagccagagc
      421 aaatcatacg gcacccagcc tttcggcaaa tttttgtctt gcgaatgcaa ccgcgcattg
      481 cggattatcc catccaaatc atccggattg cccaaaactt cccacaactt gatcgttttg
      541 gaatctttgc gaaactcttc cactgcctcg cgccaacttt tttcatattt ctttgtcggc
      601 ttcaccaatc tcaacataat tttttataaa tttcgtaatt ttcattatca aaacaaacaa
      661 aaattatttg gtcaaaacta tctgagttga cagcgcaaaa ttctttgact gccgcgagag
      721 caattttggc cgcttcattt ttaggataac catacgcgcc agtagatatt gccggaaacg
      781 cgattgtccg aatattatta tcttgcgcaa gtcccaagca atttttgtaa caatttgcca
      841 gcagtttgaa tgctttatcg tcttcttcct tgccataaac cgggcccgcc gtatgaataa
      901 catattttgc cggcagatta tagcccttgg taattttggc ttcaccggtt ttgcaaccgc
      961 caaatttttt gcattcttcc aacaattccg gcccggccgc acgatgaatc gccaaatcca
     1021 ccccgccgcc gccggacatt ttttcatcgg tggcattaac aatcgcctcc acctccaatt
     1081 ttgttatatc cccttttaca gctttaattt ccatagttat ttacaataaa aactcgggat
     1141 aattatcgcg attgatggtt ttgaattcag aattcggata agcttccaaa aattttactg
     1201 gcgctttgat ggcttccttg ctgaatttaa attcataggc aaaaagctta ccttcccttt
     1261 cttcaatata atcaatctct ttttgatcgt aagtgcgcca aaaataatta ttggaaaaaa
     1321 catcccgata atgattgcgt ttcatccgtt caagtacaca aaaattttcc cacaaaaatc
     1381 cggcatcgtt tctaatttcc aaaggattaa aattattgat caaattattg cgaatgccta
     1441 aatcataaaa ataaattttc accgatttgg tgatttcgtg gcgcaaattg cggccgaaag
     1501 aattcaaccg gaaaattatg aaacattgtt caaggatatt aacatatttt tcaaccgtta
     1561 aacgattgat attcagccga ttggccaact cgctaaaaga tacttcattc cccaattgca
     1621 acgcgagtaa ttgcaaaagc tttgccacaa cgcttgattt tttaatacct tcaaaattca
     1681 aaacatcttt gaacagatag ctgctggcaa tctcattcaa ccgcttgaac gccacgtctt
     1741 caggcgcgat aaaaacttcg gggtaaactc catagcgcaa aattttttcc aacctggcat
     1801 taatccgggc gactccgcca tttttgattt cagaaagcga aaacggataa agatcaaaca
     1861 cgattttgcg gccggtgagc ggttcgttaa tcttgttggc cagatcaaac gacgacgaac
     1921 ccgtggccaa aatttgcgtt tccggataag catcaataaa aactttcaaa attaaaccga
     1981 tatttttaat tcgttgcgcc tcgtctaaaa cgattaattt ggcgtttccc aaataatcct
     2041 tgatcatttt tggttcgggc ttggcaagcg cctcttggac actcaggacc tcacaattaa
     2101 aataactgcc ctgatctttg tattttgcca aaatttcttt ggccaaagtg gttttgccca
     2161 ctcgccgcgc gccgtagata ataatgatct tgccttggaa taattctttt tcaatttttt
     2221 cctgaatttc tcgttttatc atagatttag gttaaaatat caaaatttat ataaattttg
     2281 atattacaat atcattattt ggcaaaattc tgacattgtc aacccgcctg ttccaattta
     2341 tcaataatcc tttattttat caagataatt ttggcacgca gattgaatgt attttttatc
     2401 gttttcattc accaagcgcg caactttccc gggtaatccc ataaccacgc tgccgccttc
     2461 aattttggaa ttcgggacta ccacgctgcc cgccgcaacg atggaccaat cgccaatttc
     2521 aacaccatcc aaaataacac tgctgatgcc aatcaaaaca ttattgccaa tctttgcacc
     2581 gtgaattacc gcattatggc caatcgtaac atttttgcca atactaacgg cgccatggat
     2641 aatcgcgcca tcttgaacat tggaattatc gccaattatg attttacctt catcccctct
     2701 tattgaagca aacgcccaca cactcacatt ctctcccaaa tcaacatcgc cctccacatt
     2761 tgatttttca tgcaaaaatt gttttgccat attttaaaga attgaatctc ttaattttgc
     2821 cattgcaaat aatcattaat gatttgcctg aagttaaaac cgatgttcaa gttttgcaat
     2881 tccgcaggcg caaaaaatcg catggccgcg ccttcgccaa gattcaaatt ttccaattcc
     2941 accggctcag ccagaatgta aatcatttgc ttgatgccgc ggtcaatata ttcggcgaaa
     3001 aatcccgcgc acccgatttc aaaaccgagt tcctctttga tttcccgcgc cagcgcgttt
     3061 tccggcgttt cgccgtcttt aactcccccg ccgaacaaaa cccatttgcc gatctccgga
     3121 tcttcgtcgc gcaactgcaa caacaattct ttcttcttat tttcaacaat caaacaaact
     3181 tttgtttttg ccataaaata tataaatcaa aactttatgc aaaattgcaa aatttcataa
     3241 agtctcattg ctgtcaaact tcatctcatc caaacggaca aatttgaatt tttcttttaa
     3301 cttttcaata atttccggca aggccgcaaa catttccgcc ggccgcgttt tttgctcctc
     3361 ttctttgcgg cttccgtgat cataaatcaa cataaaaacc atttacggac attctcacat
     3421 tcttgagaat gttggaatgt ccgtaaattc aatattctcc cgatcgcatt tatttttcaa
     3481 accattcacc gccgacgaca ccgccctcgt tcttggcttc ctgaaacgcc gccttcaact
     3541 cttccaacga caaatcccga ccttgttcct gcgcggcctt cattgcccgg caaatcgggc
     3601 aatcgtcaaa aaatatctcg ccgtttttat tttccatatt tttatttttt attgctgtca
     3661 aaattatcaa aatattcggc aaataaataa aaacggatat ggcatccgtt tttattttta
     3721 aaatttcaaa ctacgcatcg ccttcgtcat cgccgtcctt tttttcttct tcgtcgtcaa
     3781 atttaaacat tgttttgcaa aattcaaccc tagaaattga atcttgcggt taatcagttt
     3841 gttatcactg acagaccttt atggcctgat tttaactttt ttgccgccga agtgtcaagg
     3901 attttaccgc ctatttcccc cggataaatt cacggatgcg atcctgaaag cgataataga
     3961 gataaccggt gaacagcaac acgcatccca taatgatgaa agaaaagaaa cggtaaaggt
     4021 ctcccaagtt ggcggtatcc accaaaaaaa cttttgcgat tacgattata aacaaacaga
     4081 ttgccgaaaa ccgttcataa atcgcttttt tggcgattcc ccaaaccatc agcaccgcac
     4141 cgtagaccat ccaagcggcg gatagcaata cgtttttcaa attttcaatt tcccgcaacg
     4201 cggtttttgc cgcgccgatt tttgccagat cgcaggcact gacaatttcg gcactaacaa
     4261 tccatagaac gagaatgtgg aaataagcaa acagcgccgg acgaaaaagc cgatattcat
     4321 ccgcggaaat tttctctttg ttgccgatca gcattgaaag aaacactctt cccaacaacg
     4381 ccgaaacgac aaacgccatc acccggaagt tgaatatggg attgtgatca gccaaaacga
     4441 cggaagtctc agaaaacaac aagcggaaga acgcaatgcc aaaagcaaaa tatgcgaccg
     4501 gacgcaaaat tccggcggat accttaaacg acagccacgc gaacaacaag ccggcggcgc
     4561 tccaaaaaat cggataccaa tagccggcaa aaaaatcacc gatttcaaga ctgcccccaa
     4621 acaaaaacat tccggcggcg gacagcgtta aaattgaaaa aacggatttt tcattttcgt
     4681 tggccgattg ccgataacgg cggtacagat acgccgcgcc ggccgcggcg ataaaaaacg
     4741 ccgcgtaagc aaaaaaacga tcattcacaa tcgggcgcgc ggttaacggc aacgactcca
     4801 taaaaaacaa cgtcttaaaa agtgtcagca tcaataagcc gttgcccatt aaacgatatg
     4861 ccccaaagtt cattttaaat ccggaactga tgaaaaccag cgcctcggcg gcccagccga
     4921 caacaatcca ttcgccgtca aattgaatgg gcacggcaat tgacagcaaa acaaatccgg
     4981 cgctcatcag aaaatgacgg aaaagcaacg attgcaaacg cgctctgccc gccatcaccg
     5041 ccagcaagca atacaaaatt cccaaaccgg cgacgaaagg tcctgataat tgcgggtaca
     5101 gcgaatcaat cacgggataa gcggcatgga aataaagcaa gggcgccaaa attacgacca
     5161 gcaagtccca agcgttctct ccggcttttt tgacgtaatg ttggataaaa gaaattgcga
     5221 ggaaaatggc aaaaaacagg ctggcataag caagcgccgg aaaaaattga tccgccgtgt
     5281 agaaattcaa gtaccaatag ccgtaagcaa gcgccgttcc gataattccg gtcaaggaaa
     5341 gcggcgccca caatttgcag aaagctgtta aaaatatcgc cagattcagc aatgcgatat
     5401 acaaaaacgg aatatgggga cttcccgcgc cgttatcaat caaaagcggc gtcaaaaagc
     5461 cgccgacctg cgcgaaaatc gccaaactca ttgaattctg cctcaaagaa agcaatatcc
     5521 ccgcggccgt aaccccgatc atggaaagaa acgccgccgg ctggacgatt agctggtaaa
     5581 aactgaaagc cgcgtaaaaa gaaacataca aaacccccaa tccgccgccg gtaagcgcct
     5641 ggccgtaatt cggatacttt ttgcgcgtca attcgcccac cgccaaaagg atcgcgcccg
     5701 caatcgctcc cagcgccacg cgcccccatt cattgatcaa atcattttca aacgcgtaac
     5761 gcagaaaaaa cccggcggcg caaataatcg ccaccacgcc cacgccggtg aaaaatttgc
     5821 ttcccaattt gaactcaaaa cttccggaat cctctttttt ggacaccggc aagggggaag
     5881 aagagtcgga gagcgcaacc ggcgtctctt tttcaatttg cgccggcatt tcggtaatag
     5941 caacttgatc cgtctttctt cttgcggcgt caaaacgcgc cctcaaagag tcaatctctt
     6001 ctcttaatcc ctcaatctgg ttttttaaat gcaacgcgac aagcaaaaat aacaaaagaa
     6061 aaataaactc catcataaat tatgtatttt atttaaaatt ttaaaaaatt catccgcgat
     6121 ccccggcgaa cccccgccaa agccaaaagc agtttcattt gataatattg tcttttaatt
     6181 tcttagcctc gccgacaaaa taattttgcc aatccttggc gacaatcttt tttttcataa
     6241 tcggataatc tttcacttca acggcacgca aaaactggct ccccaactgc aacaaatcaa
     6301 tatgagtgtc aaatttgatt ctggcttttt tggccagatc gccaacggca aaaccaaatt
     6361 cgccatgcaa gcaataaaca tcaatgaaat cgcgcgcccg gggattttgg taaatcgtaa
     6421 aaattttatt gaccgcaata tcaagcgcgc catcaattcg cagatcgccg atttcggttt
     6481 tcggttggat ctcggtaaat ggaaaatatg taaattcggc tttcacgata tcaccagcaa
     6541 agtcaacaaa gaacaaatta cggttgaaac tttgctgata atcaatcttt ccaaaaccga
     6601 taatcttttt gtttttttta aaaaaaacgc taactgcttg aacatcaaat tcattttcgg
     6661 aaaaaaaatc caaatcctca gataaacgat gatgcaaata gaattcggcc agcgccgtac
     6721 cgccggtaag gtaaaagcga tgacaaattt ctttgtcatt ggcaataatt tcaagaagcc
     6781 ttgtctgatt tgggctcaaa atggttcttg ccataatcaa ttgtcattat cttgctactc
     6841 cctgattccc aaaagcatcg ccaaatattt ttttttgcgc ggatcaatcc gcaatttggg
     6901 ccagtatttt ttcaattcgc ggccatccaa tttttgtccg tctaacccaa aattaaccat
     6961 ttgctccaac cgccaaacag cgtatttccc cttgtctttt tttaattctt taatatcaat
     7021 tgaccaattt ttcataaata gcaataaaga ttatactagg caaaatcagt ccattttaat
     7081 tattctgtca aaatcaatca tta
//
LOCUS       DAOQWE010000032         7078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_11002_length_7078_cov_6.131995, whole genome shotgun sequence.
ACCESSION   DAOQWE010000032 DAOQWE010000000
VERSION     DAOQWE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11002_length_7078_cov_6.131995"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PKK37_01895"
     CDS             complement(<1..956)
                     /locus_tag="PKK37_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81166.1"
                     /translation="MEEINQKGFAAIVVALVIAVALLAGVGIWVWQTQIAKPVPKPAP
                     VVQETGAPADEPVANVDEVGTETAEITSGQIESIDTTAWKTYRNEEYGFELKYPKKWS
                     VANNILSFPPDLVFCPNDLVDINDSVINCRIKNDNLKPSYEQGIIYLFSHDKSRYYNN
                     ANYQYLGFGGLTPRHYYLFFDVGGNEAQAKQMISTFKFIDIISRDKGVASYSMEKSEK
                     GFSFPKITVFEDENIKNKINARLKEVAAGFCECSTGVCQVGAEVTYSSNYIFSVNVSA
                     IWSCEGYLHDARVFEGLVFDMKTGNQVAIGDVFDLSLDMGSLR"
     gene            complement(960..2306)
                     /locus_tag="PKK37_01900"
     CDS             complement(960..2306)
                     /locus_tag="PKK37_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81167.1"
                     /translation="MLLVITYFNIKYVNTNMDIEKIKKIIVDQKEEIEEIYRREKIIK
                     REIDFTRLQNFLAKPNILLITGPRRSGKSTLATFILRDKRAGYLNFDDERLAGFCADD
                     FDVALRAFYELYGQDIEHFIFDEIQNISGWELFANRLRRTKKIIITGSNANLLSEELA
                     THLTGRYFEFVLYPFSFREFLDFRGIVIEENDFYSTKKISRIKKNLTEYLNCGGFPET
                     FKFGAAIAGRIYEDVIVKDILLRYGVKNKKTFREMANYVVSNFAGGLNYRKLGHVFSI
                     KDVHTVKNYIDYLSSVFLIFVFEKFSYKLKQRYITSKKVYAIDTGMINEVAVNFSDNF
                     GRIMENAVAIDLRRRKNYWQEKSKIFYWSDYSGKEVDFAVKNKNKITQLIQVCHKLDA
                     MGPKEREIKALVLASDELKCDNLLIITDDEEGVEKIDGKKVIKIPLWKWLLIDYKK"
     gene            complement(2513..3148)
                     /locus_tag="PKK37_01905"
     CDS             complement(2513..3148)
                     /locus_tag="PKK37_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81168.1"
                     /translation="MEDWNSKKECSGCDCRKKTIVAAATFLVLVAAAIFLLVRQAQSQ
                     PFFGAEWKNFRENALGISFTYPSSWNLIADYDHYAPGIFSAEMDNKKCAGASGKCGSD
                     CIDLRILAAKKPLDGRGDSLVVQLYEDFMMVRDFSVSPLVAEIDSGSKKVFQVDHGGP
                     TLALNGACAGPLYLFEAGDYFVYVFAGYGDSASGENDKIREIINSIRIGGA"
     gene            complement(3225..>4319)
                     /locus_tag="PKK37_01910"
     CDS             complement(3225..>4319)
                     /locus_tag="PKK37_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81169.1"
                     /translation="WKTYRNEEYGFELKYPQSWTFDDGSFGDYTLSQNRSSIQINFSN
                     GAKGPEDESQSICQPGYAAGMFQIGIKRVDGTGRPHDDLGFQEFVNFIIKNPERGTAP
                     GTMPVLEPMIFGGRAALKYVDRVKDCDKTNYYIDQAPNLYAAGMTISANNEDKPIMDK
                     IISTFKFTEVNSEQEPIASSHKLLLESSDFCGEEQGKIFNEDSNYDVGGAKISYGGCI
                     IEGMPGSTLSILSTNGRGNTSTIWRLNGDRWKGVSVVEAKDIDGDGIKEFAYGGYNYG
                     GTCTGRIDELCLYSPVKNEHFCVSEREGWGNYSSPNLKVEFCDNLQVIAEDIGCGKAP
                     YHFVCFSNNFKNEQNEIFKDYLKNKMDSSR"
     assembly_gap    4320..4419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4714..5190)
                     /locus_tag="PKK37_01915"
     CDS             complement(4714..5190)
                     /locus_tag="PKK37_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018998977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNR81170.1"
                     /translation="MLKDKINEDFKAAFKEKKEGELSVLKNLKAALLNKEKEKQFQAS
                     KAGEDIAQAVLADDDIIAVISAEVKKLRDAIALFEQGGRADLVAQNKGEIEVLLRYLP
                     EQLGEDEIKKLVAGAIAQAGATTVKDMGKVMGILMPKIKGRADSGMAGKLVKEALG"
     gene            complement(5215..5430)
                     /locus_tag="PKK37_01920"
     CDS             complement(5215..5430)
                     /locus_tag="PKK37_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81171.1"
                     /translation="MALEIRRKQRENAQAMMRRFQKAVQQSGILLEKRRRQFAKRAKS
                     DNMQKRSALRKIETKKKYDLIKKMRKA"
     gene            complement(5466..5807)
                     /locus_tag="PKK37_01925"
     CDS             complement(5466..5807)
                     /locus_tag="PKK37_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNR81172.1"
                     /translation="MECVFCKIINKEIPAKVVWENDRVLAFENIKPVHPVHVLIIPKK
                     HIASIQEAEAQDGEYLSQILLAVSEIAKIKGVAQSGYRLIINCGKDSGQSVDHLHVHL
                     VGGEELPFATG"
     gene            complement(5840..>7078)
                     /gene="hisS"
                     /locus_tag="PKK37_01930"
     CDS             complement(5840..>7078)
                     /gene="hisS"
                     /locus_tag="PKK37_01930"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNR81173.1"
                     /translation="ILPDDQKYYEKIYETAKEIADFYGFSRIETPILEDSDLFIKGTG
                     MTTEIVQKQMFVFKTKGNDNVALRPEGTPPVVRAYLQHGMANWPPPVKLWYWGPFFRY
                     EKPQAGRYRQFWQLGFEAIGQGGAIIDAQIIQIYFNLLRELGLKDIAIEINSIGDAVC
                     RPQYRRALAGYLKNRQNSLCADCRRRVKENPLRVLDCKEEKCQSVKAQAPQAVDHLCD
                     ECKKHCQEVLEYLEELGLPYRLNPYLVRGLDYYTRTVFEMFREGEGEKAPVALAGGGR
                     YDALVKMMGGKETFAVGGGMGVDRVVLAMKAADIEIGKDKKVKVFLAQLGGLGKKKSL
                     KLFEELRRAQIPTGEAFDRDSLKSQLRVADKYGARYALLLGQKEALENTVILRDMESG
                     KQEVLNFDAAVAEVKKRLRK"
BASE COUNT         1813 a         1827 c         1302 g         2036 t
ORIGIN      
        1 cgcagacttc ccatatccag cgacaaatca aaaacgtctc caatcgcgac ttgattcccg
       61 gttttcatat caaaaaccaa accttcgaat actctcgcat cgtgcaaata accttcacaa
      121 gaccaaatcg ccgaaacatt tacgctgaaa atataatttg aactatatgt tacttcggca
      181 ccgacttggc atactcccgt tgagcattcg caaaaaccag ccgccacttc tttgagccgc
      241 gcatttattt tatttttaat attttcatct tcaaaaacgg ttatcttggg aaaactgaat
      301 cctttctcgc ttttctccat tgaatatgac gctactccct tatcccttga aatgatatca
      361 ataaatttga aagtggaaat catttgtttt gcttgcgcct cattcccgcc aacatcaaaa
      421 aagagataat agtgccgggg agttaatcca ccaaacccga gatattgata atttgcgtta
      481 ttataatagc gactcttatc atgagaaaac aaatatatta ttccctgctc atacgacggc
      541 tttaaattat catttttgat tctgcaatta attaccgaat cgttgatatc aaccaaatca
      601 ttggggcaaa aaaccaaatc cggcgggaag cttaaaatat tatttgcaac gctccatttt
      661 ttaggatatt ttaattcaaa tccgtattcc tcgttgcggt aggttttcca ggcggtggta
      721 tcaatagact cgatttggcc ggatgtgatt tcggcggttt cggtccccac ctcatccacg
      781 tttgccaccg gttcatccgc cggggctccg gtttcttgca ccaccggcgc gggttttggc
      841 actggcttgg caatttgcgt ttgccaaacc caaattccga cgccggccaa aagcgccacc
      901 gcaataacca gcgcgacgac aattgccgca aatccttttt ggtttatttc ctccataatt
      961 tattttttat aatcgattaa aagccacttc cacaaaggaa tttttatcac ttttttgccg
     1021 tcaatttttt ccaccccttc ttcatcgtcc gtaataatca ataaattatc gcatttcaat
     1081 tcatcagaag ccaaaacaag cgcttttatt tccctttctt ttggtcccat ggcgtctaat
     1141 ttatggcaaa cttgaataag ttgcgttatt ttgtttttat tttttacggc aaaatcaacc
     1201 tccttgccgg aataatcgct ccaataaaat atttttgatt tttcttgcca ataattcttc
     1261 cgccttctaa gatcaatcgc caccgcgttt tccatgatcc gaccgaaatt atccgaaaaa
     1321 ttaaccgcaa cttcgttgat cattcccgta tcaatcgcgt aaactttttt ggaggtgata
     1381 tacctttgtt tcaatttata cgaaaatttt tcaaaaacaa aaattaaaaa aaccgaactc
     1441 aaataatcaa tgtagttttt aaccgtatgc acgtccttga tggaaaaaac atgccccaac
     1501 ttgcgatagt tcaagccacc ggcaaaattg gaaacaacat aattggccat ttcgcgaaaa
     1561 gtttttttat ttttcacgcc ataacgcaat aaaatatcct tgacaataac atcttcataa
     1621 atcctaccgg caatcgccgc gccaaattta aacgtttcag gaaatcctcc acagttaagg
     1681 tattcagtta aatttttctt tattcgtgaa attttttttg ttgaataaaa atcattttct
     1741 tcaatcacaa tacctctaaa atccaaaaat tcgcgaaaag aaaacgggta taagacaaat
     1801 tcaaaataac gtccggtaag atgcgtcgcc aattcttccg acaacagatt agcatttgaa
     1861 ccggtaataa ttattttctt ggttcgccgc aagcgattgg caaacaactc ccatccggaa
     1921 atattttgta tctcatcaaa aataaaatgc tcaatatctt gcccgtaaag ttcgtaaaac
     1981 gcccgcaatg caacgtcaaa atcatcagcg caaaatccag ccagccgttc gtcgtcaaaa
     2041 ttcagataac cggctctctt gtccctcaaa ataaacgtag ccaatgttga ttttccgctt
     2101 ctccttggcc cggtgatcaa aagaatattg ggcttggcca aaaaattttg caatcgcgta
     2161 aaatctattt cccgctttat tattttttcg cgccgataga tttcttctat ttcttctttt
     2221 tgatcgacga ttatcttttt aattttctca atatccatat tcgtattcac atattttata
     2281 ttaaaatatg tgattactaa caacatattt taaactatca cggacggcca cgaattgtca
     2341 acatcaatcc ttaaacctgt tagaaacagg ttcattattt ttataaatga tttttattca
     2401 tccgccggaa tatcccggaa atttgctccg gcgtggttta ttttttttcg ccaaacacca
     2461 acggcaaaaa acggaagggt tttgcttccg ccgcgcagga gacgcgtgcc agtcacgcgc
     2521 caccaattcg gattgaattg ataatttcac ggattttgtc gttttcgcca ctggcggaat
     2581 cgccatagcc ggcaaaaaca taaacaaaat aatcgccggc ctcaaacagg tagagcggac
     2641 cggcgcaggc gccgtttaac gccaacgtgg gaccgccatg gtccacttga aaaacctttt
     2701 tggaacccga atcaatctcg gccaccagcg gggaaacgga aaaatcgcgc accatcataa
     2761 aatcctcgta aagctggacc accaaactgt ctccccggcc gtcaagcggt ttttttgccg
     2821 ccagtatccg caagtcaatg cagtcggagc cgcatttccc ggaagcgccg gcgcattttt
     2881 tgttgtccat ttccgcggaa aagatgcccg gcgcgtaatg atcgtaatcc gcaatcaaat
     2941 tccaacttga cggataagta aaagaaattc cgagagcgtt ttcgcggaaa tttttccatt
     3001 ccgcgccgaa aaacggttgg ctttgcgctt gcctgaccaa cagaaaaatc gcggcggcca
     3061 ccagcaccaa aaacgtggcg gcggcaacaa tcgttttttt gcggcaatcg catcccgaac
     3121 attctttttt tgaattccaa tcttccattt ccgcatttta acatatcgcc gccttgaatc
     3181 aattcttgcc aaattcaatt gcttattgca ccgcgcggat caaatcagcg gctcgaatcc
     3241 attttgtttt tcaaataatc cttgaaaatt tcattttgtt cgtttttaaa attgtttgaa
     3301 aagcaaacaa aatgataagg cgcctttccg caacctatat cctcggcgat aacctgcaaa
     3361 ttatcgcaaa actccacctt caagttgggc gaagaataat ttccccatcc ttccctttcg
     3421 ctgacgcaaa aatgttcgtt tttaaccgga gaatacaaac acaactcgtc aatccgcccg
     3481 gtgcaagttc ctccataatt ataaccgcca tacgcaaact ctttaatgcc gtcgccatca
     3541 atatcttttg cctcgacaac actgacgcct ttccatctgt ccccgttgag ccgccagatt
     3601 gttgaagtat taccgcgccc gttcgttgac aatattgaca acgttgaccc cggcattcct
     3661 tcaattatgc aaccgccata ggaaattttt gcgccgccaa catcataatt gctgtcttca
     3721 ttaaatattt tcccttgctc ttcgccgcaa aaatcagagg attcaagcaa aagcttgtgt
     3781 gaagaagcaa tcggctcctg ctctgagttc acctcggtaa atttgaaggt ggaaataatt
     3841 ttatccataa ttggcttgtc ctcattgttt gcgctaatcg tcattccggc cgcataaaga
     3901 ttcggcgctt gatcaatata ataattcgtt ttatcgcaat cttttaccct atcaacatat
     3961 tttaaagccg cgcgccctcc gaaaatcatc ggctccaaaa cgggcattgt cccgggcgcc
     4021 gtgcctcttt ccgggttttt aatgatgaaa tttacaaatt cttgaaaacc taaatcatcg
     4081 tgcggccgtc ccgtgccgtc aacccgcttg atgccgattt gaaacattcc cgccgcatat
     4141 cccggctgac atatgctttg cgattcgtcc tccggccctt ttgcgccatt tgaaaaatta
     4201 atttggatag atgaacggtt ttgggaaaga gtataatcgc caaacgaccc gtcatcaaaa
     4261 gtccacgatt gaggatattt caattcaaac ccgtattcct cgttgcggta ggttttccan
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggtttcttgg accgcgggaa
     4441 cggctggcga cgttggcttg ccgatttgcg tttggtaaaa ataaaccccg gcgccgacca
     4501 aaagcgtggc cgcgactatc agcgcgacag caatcgtcgc aaatccgttt tgattttttt
     4561 tcattgatgt ttattttaat ttaaattatt tcattttaac aaatcaacgc caaaaaaaca
     4621 gacgccgccg cttaaatcaa aaaacagggc atgccctgtt ttgttttgta tttttgcatt
     4681 tgcaggagcg aataatcatt cgcccttacg cgatcatccc aacgcttctt tcaccagctt
     4741 tcccgccatt ccgctgtcgg ctctaccctt tattttgggc atcaaaattc ccatcacctt
     4801 gcccatatct ttgacagtgg tcgcgccggc ctgcgcaatc gcgccggcga caagtttttt
     4861 aatttcgtct tctcccaatt gctcgggcag ataacgcagg aggacttcaa tctctccctt
     4921 gttttgcgca accagatcgg cgcggcctcc ttgctcaaac agcgcgatgg catcgcgaag
     4981 ttttttaact tcggcggaga ttacggcgat gatatcgtca tcggcaagca ccgcttgagc
     5041 aatgtcttcg cccgccttgc tggcctggaa ttgcttttct ttttctttgt taagaagagc
     5101 ggctttaagg ttcttcaaaa ccgaaagttc gccttctttt ttttccttga acgccgcctt
     5161 gaagtcttcg ttgattttat ctttcaacat tattggaagc aaaaaaatgc gccgctaagc
     5221 tttgcgcatt tttttgatta aatcgtattt tttcttggtt tcaattttgc gcagggcgga
     5281 tctcttttgc atattgtccg attttgcccg cttggcaaac tggcgccgcc gcttttccag
     5341 caaaatgccg ctctgttgca ccgctttttg gaaccggcgc atcatcgctt gcgcgttttc
     5401 gcgctgtttt cttctgattt ccaaagccat aagatttatt aattataagt tataaattat
     5461 caattctagc cggtggcgaa cggcaactct tcgccgccca ccaagtgaac gtgcagatgg
     5521 tccaccgact ggccggaatc tttgccgcaa ttgatgatca ggcgatagcc gctttgggcc
     5581 acccccttga tcttggcgat ttcggaaacc gccagcaaaa tttgcgacaa atattctccg
     5641 tcttgcgctt ccgcctcttg gatgctggca atatgtttct tgggaataat caacacgtgc
     5701 accggatgca ccggcttgat attttcaaac gccagcaccc ggtcgttttc ccacacaact
     5761 ttcgccggaa tctctttgtt aatgattttg caaaacacgc actccatata tttacctcaa
     5821 tttcggttat tcgtcaacct cacttccgca ggcgtttttt cacttccgcc accgcggcgt
     5881 caaaattcaa aacctcctgt ttgcccgatt ccatatcgcg caggatcacg gtgttctcaa
     5941 gcgcttcttt ctggcccaag agcaacgcat accgggcgcc gtatttgtcg gcaaccctca
     6001 attgcgattt caacgaatcg cggtcaaacg cctcgccggt cggaatttgg gcgcggcgca
     6061 actcttcaaa aagtttcaat gattttttct tgcccaaccc gcccagttgc gccaaaaaga
     6121 cttttacttt tttgtctttg ccgatttcaa tgtccgcggc tttcatcgcc aaaacaacac
     6181 gatcaactcc catgccgccg ccaaccgcga aagtttcctt gccgcccatc attttaacca
     6241 gcgcgtcata gcggccgccg ccggccagcg ccaccggcgc cttttctccc tctccttccc
     6301 ggaacatttc aaagaccgtc cgcgtataat aatccaaacc gcgcaccaaa taaggattca
     6361 ggcgataggg caagccaagc tcttccagat attccaaaac ttcctggcag tgttttttgc
     6421 attcgtcgca aagatgatcc accgcctgcg gcgcctgcgc tttgaccgat tggcattttt
     6481 cttctttgca gtcaagcact ctcagcggat tttctttcac ccgccgccgg caatcggcgc
     6541 aaagggagtt ctggcggttc ttcagataac cggccagcgc ccgccgatat tgcggccggc
     6601 aaaccgcgtc gccaatgcta ttgatttcaa tggcgatatc cttcaagccc aactcgcgca
     6661 acaaattaaa gtatatttga atgatttgcg catcaataat cgctccaccc tggccgattg
     6721 cttcaaagcc caactgccaa aactggcgat aacggccggc ctgaggtttt tcataacgga
     6781 aaaacggccc ccaataccaa agtttgaccg gcggcggcca attggccatc ccgtgttgca
     6841 aataagcgcg gacgaccggc ggcgtcccct cgggacgcaa cgccacattg tcgttaccct
     6901 tggtcttgaa aacaaacatc tgtttttgca caatctccgt ggtcataccc gtgcccttga
     6961 tgaaaagatc gctgtcttcc aaaatcggcg tttctatccg cgagaaaccg taaaagtccg
     7021 cgatctcttt ggcggtttca tagatttttt cgtaatattt ctgatcgtcg ggcaaaat
//
LOCUS       DAOQWE010000033         7076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_11010_length_7076_cov_21.764136, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000033 DAOQWE010000000
VERSION     DAOQWE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11010_length_7076_cov_21.764136"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKK37_01935"
     CDS             complement(<1..644)
                     /locus_tag="PKK37_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNR81174.1"
                     /translation="MKTKYFLYARKSTEDEERQVMSIEAQLAELADFAKRENLDIAER
                     FIESKSAKKPGREIFNEMIQKIYASKEPVGILAWHPDRLARNSVDGGQVIYLIDIGKI
                     ASLRFPTFWFEPTPQGLFMLQVAFGQSKYYSDNLSENVKRGIRQKLRRGEWPGLAPFG
                     YVNNPKTRTIEPDQVKARVIKKAFEEYAQGRHTLESLGQRLSFWGVVSGTGKPL"
     gene            760..1035
                     /locus_tag="PKK37_01940"
     CDS             760..1035
                     /locus_tag="PKK37_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81175.1"
                     /translation="MESKNNSMNFLKVCFCSGGFAAPNRRLLDRKLIFDFVRPFAFVP
                     KYTGKCERSRAKSAASEQTNCPQNSQCLDWSQLLNEMRTHFEQEFLP"
     gene            1486..1695
                     /locus_tag="PKK37_01945"
     CDS             1486..1695
                     /locus_tag="PKK37_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81176.1"
                     /translation="MKAMSNKISENLKKLREKKGYSLEKVARLADLSLNTIVKVENGV
                     NKNPTIETLTKIAKALEVGVDDLIK"
     gene            1698..2927
                     /locus_tag="PKK37_01950"
     CDS             1698..2927
                     /locus_tag="PKK37_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81177.1"
                     /translation="MNKHIPLLEKLFSKDYQLIDGTDEIFELDLALWEYEALSKKELI
                     DRSAYLKSVDGKETTHFRTCNLQNLEEVHKNSSFRTKVFFLDGKYSTGYATHSLFPYR
                     GKFHPQLIRALLNILNIKQGHIVLDPMAGSSTVAVEANLLGIDAISVDLSPFCELMGK
                     VKTFALNLDINILESIVANPKEILEKLKKDRVPEYFKNNKDDKKRSYYEIVLLAFLDT
                     VGFAGRSNSSIDKLFTRVLERYISTIKYFQEARQKMNLKIGRSKIIQGSVMKLPIDDN
                     SIDAIITSPPYSFAIDYLKNDQPQLEYLGHNLEILRQEMIGLQGRGVENKLEIYFDKM
                     DRALGEMKRVSKKNAPVVIIIGTNDIQTNGVRLETKVIELGEKQGLKFELNFKKPIRG
                     LQNTMKEESILFFTNQK"
     gene            2932..3921
                     /locus_tag="PKK37_01955"
     CDS             2932..3921
                     /locus_tag="PKK37_01955"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HNR81178.1"
                     /translation="MKHTKPTTDIKFKTVIDKNTFYFFNPAFEEKYEGYLNSLKEMLL
                     ILKNNIETQGLKKEFFEQLLIEKENGLRALLALTGFSNETLKRLTTIIRVVNDPELSK
                     LVYKDKWFRDEDINNLKEWSDGQIAKFIKENEFFRKGILNIFFEGSTVPFLTKTVPLF
                     ELKKLSISKLKFELPEMIDTLIRYKEKGSYSGMKENNPEILIEEILNHLEIPFEKGDL
                     GELIDNAPDNKRTMDFIIPNKQNPLIIAESSFLVTTSSGQGDKSKTEISIDSLIKSHY
                     PKAKFIGFVDGIGWYVRKGDLKRMVTAYEDVFTFHKEELKRFEKLLIGTFLTK"
     gene            3924..4826
                     /locus_tag="PKK37_01960"
     CDS             3924..4826
                     /locus_tag="PKK37_01960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81179.1"
                     /translation="MINKFINKIIQDDCLAALKKIPDNSVDMTFADPPFNLKKKYNHY
                     EDSKEKQDYLNWCNDWISEMVRVTKSTGAIFVHNIPRWLSHYAEHLNKIAYFRHWIAW
                     DSGGAPMGKTLLPNHYGVLYYTKSKSHKDFKFFDIRYPHPRCRVCGEFLKDYGGKKDQ
                     AHGFGPLLSDVWNDIHRIRHKKRRDEHPCQLPVPLLERLILMTTDEGDIVLDPFVGTG
                     TTAVAAKRLGRKYIGIELDPDYIKISEKNLKAAKETKINGHFVSIYLGHIRTMRDKDF
                     KEIWKDKGDLKLLDRKMYKITKRI"
     gene            4844..6475
                     /locus_tag="PKK37_01965"
                     /pseudo
     CDS             4844..6475
                     /locus_tag="PKK37_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04141.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
BASE COUNT         2337 a         1265 c         1491 g         1983 t
ORIGIN      
        1 caaagcggtt ttccggttcc gctcaccacg ccccaaaaac tcaggcgttg gcctaagctt
       61 tccaaggtgt gtcggccttg cgcgtattcc tcaaacgctt ttttgataac tcttgctttc
      121 acttggtcgg gttctatcgt tcgcgttttt ggattgttca catagccgaa cggcgcaagg
      181 ccgggccatt cgccccgcct gagtttttgt ctaattccgc gcttaacatt ttccgacaaa
      241 ttgtcggaat agtattttga ctggccaaag gcgacttgaa gcataaacaa tccttgcgga
      301 gttggctcaa accaaaaagt gggaaagcgc aaagaagcga tcttgccgat gtctatcaag
      361 taaatcactt gcccgccgtc aacggaattg cgggcgaggc ggtcggggtg ccacgctaaa
      421 attccgaccg gctctttgct cgcgtaaatt ttctgtatca tctcgttgaa aatttctcgg
      481 cccggcttct ttgcgctttt gctttcaata aaccgttcgg caatatcaag gttttctctt
      541 ttggcaaagt ccgccaactc cgcaagttgc gcttcaatac tcattacttg cctttcttcg
      601 tcttccgttg atttgcgagc gtaaagaaaa tattttgtct tcatagtgtt ttcactcctt
      661 aatgttaata agtcgccttt ggccgttccg ccttcggcgg aaaaagcgcg ggattaaaaa
      721 actggcttcg taatgccgca aagcggcacg cggaaaaaaa tggaaagcaa aaacaattca
      781 atgaatttct taaaggtgtg tttttgctct ggcggctttg ccgccccgaa ccgccgcctg
      841 ctggacagaa aattgatttt tgattttgtc cgtccgtttg cttttgtgcc gaaatatacg
      901 ggaaaatgcg agcgaagccg cgccaaaagc gcggcgagcg agcaaacaaa ctgcccgcaa
      961 aattcgcaat gtttagattg gtcgcaatta ctgaacgaaa tgcgaaccca ttttgagcaa
     1021 gaatttctgc cctgacgggc agaaacccac cccagcggct cgccgcctga cggcggacaa
     1081 agcccgcaaa aatttctttt tcttttaatg ggcgcgcgca aaattctttt tcaattaagg
     1141 aaacaatttt tgcgggcttt gtcttcctgc ttgagcagga acgagccgct tcccacccct
     1201 gccgcgcctt cggcgcggaa agcaggcggg caaaaattcc ttcccccaga cccccttcct
     1261 ttttgcccgc ctgctggaaa cccccagcga ttttttcgga cggcggcggg actaaaagaa
     1321 taaaagaaag aaaagtcacc cttgccccaa ccctcccgtg cgcgggcgac aacaaggaac
     1381 tcccttgagg atttttgttg actgtttttg aaaaattgtt tatcattata atagaaatca
     1441 aagaaggttt agattatatt ctatatacta taatgttaaa caataatgaa agcaatgtca
     1501 aataaaatct ctgaaaacct gaaaaagcta agagaaaaga agggctactc tcttgaaaag
     1561 gtagctcggc ttgcggattt atcgcttaat accatagtta aagttgaaaa tggagtaaat
     1621 aaaaacccga ccattgaaac attaaccaag atcgccaaag cgttagaagt tggcgtagat
     1681 gacttaatta agtgattatg aataaacata ttcctttatt ggaaaaatta ttttcaaagg
     1741 actatcagct tattgatggg actgatgaaa tttttgagtt agatttagct ttatgggaat
     1801 acgaggcttt atcaaaaaaa gaattgattg ataggagtgc ttatttaaaa tcggttgacg
     1861 gaaaagaaac aactcatttc agaacatgta atcttcaaaa tcttgaagaa gttcataaaa
     1921 atagttcgtt caggacaaaa gttttctttc ttgacggaaa atattcgacg ggctacgcaa
     1981 cgcatagtct ctttccatat cgcgggaaat ttcatcctca gttgattaga gcattgttga
     2041 atatacttaa tataaagcag ggtcatattg ttcttgatcc aatggccggc agttcaacgg
     2101 tcgcagttga agctaatttg cttggtatag atgcaatctc tgttgattta agtccttttt
     2161 gtgagttaat gggaaaagtt aaaacattcg cgttaaattt ggatataaat attttagaat
     2221 caattgtcgc aaatccgaag gaaattttag aaaaactaaa aaaagacagg gttccggaat
     2281 attttaaaaa taataaggat gataaaaaaa gaagttatta tgaaatagtg ctattggcct
     2341 ttttggatac tgtcggtttt gccggtcgta gcaattcatc tattgataaa cttttcacgc
     2401 gcgtattaga gcgatatatt tcaacaataa aatattttca agaagcaaga caaaaaatga
     2461 atttgaaaat cgggagaagt aaaataattc aaggcagtgt tatgaaattg ccaattgatg
     2521 ataacagcat agacgcaata ataacctcgc caccatattc tttcgctatt gattatttaa
     2581 aaaatgatca gccgcaatta gaatatctcg gacataatct tgaaatttta cggcaggaaa
     2641 tgattggatt acaggggcga ggcgtggaaa ataagttaga aatttatttt gataaaatgg
     2701 atcgagctct tggcgagatg aaacgagtat caaagaaaaa tgcgcccgtt gtaataatta
     2761 ttggcacaaa tgatattcaa acaaatgggg tgagattgga aacaaaagta attgaacttg
     2821 gcgaaaaaca aggacttaaa tttgaactca attttaaaaa gcccataagg gggctacaaa
     2881 atacaatgaa agaggagtca atattatttt ttacaaatca aaaataacat tatgaaacac
     2941 acaaaaccca caaccgatat taaatttaaa acagtcattg ataaaaatac tttctatttt
     3001 ttcaatccgg cctttgaaga aaaatatgaa ggttatttaa actcattaaa ggaaatgctt
     3061 ttgatattga aaaataatat tgaaacgcaa gggctaaaga aagaattttt tgaacagtta
     3121 ttaatagaaa aagaaaatgg ccttagagca ttacttgcct taactggatt ttcaaacgaa
     3181 accttaaaga gattaaccac gattatcagg gttgtaaatg atccggaatt gagcaagtta
     3241 gtctataaag acaaatggtt tcgggatgaa gatataaata atctgaaaga atggtcggac
     3301 ggacaaatcg ctaaatttat caaagaaaat gaatttttta ggaaaggaat tttaaacatt
     3361 ttctttgaag gatcaacggt gccgttctta acaaaaactg tcccactttt tgaattgaaa
     3421 aaactcagta tttcaaaact taaatttgaa ttgccggaaa tgattgatac tttaatccga
     3481 tataaagaaa aaggcagtta ttccggaatg aaagaaaata atcctgaaat tttaattgag
     3541 gaaattttga atcatttgga aataccattt gaaaaaggcg atctcggaga actcattgac
     3601 aatgcgcccg acaataaaag aaccatggac tttatcattc caaataagca aaatccgtta
     3661 atcattgccg aaagctcatt tttggtgacg acttcatcgg ggcagggcga taaatcaaaa
     3721 accgagattt ccatagattc gcttattaaa tcccactatc caaaggcaaa atttattggg
     3781 tttgtggacg gcattggctg gtatgtcagg aagggcgacc taaagagaat ggttacggcc
     3841 tatgaagatg tttttacttt tcataaagaa gaattgaagc ggtttgaaaa acttttaatc
     3901 gggacatttc ttactaaata gttatgataa acaaatttat caataaaata attcaggatg
     3961 attgcttggc ggccttaaaa aagataccgg ataattcggt tgacatgact ttcgccgacc
     4021 cgccttttaa tttaaagaaa aaatacaatc attacgaaga ttcaaaagaa aaacaagatt
     4081 atttaaattg gtgcaatgac tggataagtg aaatggtaag agtcactaaa tcaacagggg
     4141 caatttttgt tcacaatatt ccccgctggc tttcacacta cgcagagcat ttgaataaaa
     4201 ttgcatattt tagacattgg attgcttggg actccggcgg cgcaccgatg ggaaaaacct
     4261 tattgcctaa ccattatgga gttttatatt acacaaaatc aaaatctcat aaagatttta
     4321 aattttttga cattaggtac ccgcacccaa gatgtagggt ctgcggtgaa tttttaaaag
     4381 attatggcgg gaaaaaagat caggctcatg gattcgggcc gcttctttcc gatgtttgga
     4441 atgacataca ccgaatacgg cacaaaaagc gaagagacga acacccctgt caacttcctg
     4501 tgccgcttct tgagaggtta attttaatga ctacagatga gggcgatatt gtgcttgatc
     4561 cttttgtggg aacggggaca acagcggtag ccgcaaaacg acttggaaga aaatatattg
     4621 ggatagaact tgatcctgat tatataaaaa tttccgagaa aaatttaaaa gcggcaaagg
     4681 aaacaaaaat caatgggcat ttcgtttcaa tttatctcgg acatattaga acaatgagag
     4741 acaaggattt taaggaaatt tggaaagata aaggcgattt aaaattgctt gatagaaaga
     4801 tgtataaaat tacgaaaaga atatagccgt gaaaaaatca attatgcctg atgtcccaac
     4861 aaacaaacta tcggtttacc ttattaaaga ggaatactcc gaacataagg atattttgaa
     4921 aaaatttgat acgcttcaaa caaaagatgt taatggagtt ggtattcttt actttggcga
     4981 atctcatact ttcaagcctt tatggataga caaatttttt ggttccgctt tgggcgaaga
     5041 cataaaaaaa ttatttaatg cttcctctaa agcaatttta ttggcaaagg ttaaagttgc
     5101 aacaaataaa gaaagggtat ttgcaattcc gtttggctat ggctggacat ttcttaatca
     5161 tggcatttag gaagaaagat ttggcttgaa gataaccttg aataccgttg acccaaacaa
     5221 tctgcggaga attgataaga aaaatatgtc ttcggtgcca aaagacacaa gcgagcaatt
     5281 aagcagagaa ggagttgccg ccgatttcgg cattgatatt gaacaagatt taattcagtc
     5341 aatcaccggc aagaccaaag aagaaggttt cggaaaaact atcacgggca aggattcgtt
     5401 gagtttgtcc gtaaaagttg atttgtccaa cattcaagag tttctaaaac aagcatacaa
     5461 aaaatatgaa agcgatgaat acaaaaaaga ttttggctgg atagaccaga tttcggaagt
     5521 gaaagaccca aaaataattg aaaaccttaa caagggattg gttgataaca taaaaaacaa
     5581 taattttgat aaggtgtgga tggccgtgcc tgaactggtt gattgggcag atgtatccgg
     5641 cttctcttat aaaaacgaca aagacgacct caaagccgac atacttttgg ccgatttcat
     5701 ttcaaatttg ccggactcgg aaaaaactaa tttaactctg gacgctttca ataggcgtgt
     5761 ttattgtttc ggcgcaagta gcgatgaatt aaaacatcaa tggaaggctt ttaactgttt
     5821 gtattgcgaa atacaagatg acgcgaaaaa gaaaacctat cttttgagca atggtaaatg
     5881 gtatgaagta gaaaatgatt ttgctaagca ggtaaatgac ggttatcagc aattaaggaa
     5941 tagcaccgct tcggtttcct tgccagccta caaatgcaaa aacgaaaacg agtataacaa
     6001 ggaaacggca aaaaataaca ccgacttttg ttgtatggat agagaattga tcagtcatgg
     6061 cggcggttat agcaaaatag agttttgcga tttatacacc aaagataaaa aaataattca
     6121 tgtaaagcat tatggcggtt ccgccgtttt gagtcatttg ttttcgcagg gtgtcgtttc
     6181 cggtgagtta ttgcttgccg ataaggattt tagagacaaa gtaaatgaaa aactgccaaa
     6241 tggttataag attaacaatc cgaacgataa gccaaatccc tcggattttc agattatttt
     6301 tggtataata agttcgtcgt ccaaagagtt ggaaattcca ttttttagca aggttagctt
     6361 gagaaacgca aaaagaagat tggaaacata cggatataag gtttcattgc agaagattag
     6421 cgcggaggaa acagaaaaag caaaaaaaac taaccccacc caccgcccaa tttgaagtcc
     6481 cgccgccgtc cgaaaaaatc gctgggggtt tccagcaggc gggcaaaaag gaagggggtc
     6541 tgggggaagg aatttttgcc cgcctgcttt ccgcgccgaa ggcgcggcag gggtgggaag
     6601 cggctcgttc ctgctcaagc aggaagacaa agcccgcaaa aattgtttcc ttaattgaaa
     6661 aagaattttg cgcgcgccca ttaaaagaaa aagaaatttt tgcgggcttt gtccgccgtc
     6721 aggcggcgag ccgctggggt gggtttctgc ccgtcagggc agaaattctt gctcaaaatg
     6781 ggttcgcatt tcgttcagta attgcgacca ataacttatt aattttatgc attgtttgtt
     6841 ttccttgctc tcggagtatt ctcggggcaa aatcactttg cccctgtttt ctaaagctga
     6901 tttaattctc tgcggtatgc gcagagacgt actttcttgt catgttcatg gtggagtgtc
     6961 cgtatatatc tgacacggtt ttaagatcaa caccctcaag aatgatatat ggaatgtttg
     7021 cggcttcgtg ttcaggatgt tgattttggt cgcaatggaa tcattgtacg tgacgg
//
LOCUS       DAOQWE010000034         6526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_12188_length_6526_cov_7.406583, whole genome shotgun sequence.
ACCESSION   DAOQWE010000034 DAOQWE010000000
VERSION     DAOQWE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12188_length_6526_cov_7.406583"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1553
                     /locus_tag="PKK37_01970"
     CDS             <1..>1553
                     /locus_tag="PKK37_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNR81180.1"
                     /translation="MISIKKHKFGFLFAVLVIALVSAAIGNQMKNGAQSQTEESIPAV
                     KLIAVGDFREQKTVRVEGGTVQSLDQADLKSQISAQITAINVKLGDQVQAGQIIMQLQ
                     NSDISAQLEQARARLDELKKGARDEELNLARTANNEAKAALVNSIKDAYAKSDDAIHN
                     HIDKFFTNPRGKNIRFSIILNVGGSQVSFYPSDSDLGQRVADRKYKIEAMLDEWKDLL
                     AGADAAADDADIENILAVSKKNLQEEIDFANEMAPLVNSLSSDNSTYKTIIDGYKTEF
                     SAARSAINGSLASLQGALTARETARQTLELKLAGASPEQLRQAQAAVDALESTLAKTS
                     IVSPISGQISYINGRVGELAGGGSLVAGVVNPGALQIKAYVSENDLREIAEGAAVKID
                     GGGTGILQTISPAADSQTKKSEINVLIAKPGNAPIIVGQTVGFEIAAKGGADPNAYLL
                     PIEAIQFTANGNYVLTVGAESALERIPVTTGDIVGEKIYVVQGLDGDAKIISSTRGLK
                     SGDIIKVEN"
     assembly_gap    1554..1653
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1654..5022
                     /locus_tag="PKK37_01975"
     CDS             <1654..5022
                     /locus_tag="PKK37_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNR81181.1"
                     /translation="QLYLNRLKFKPELKKMWLSFFVYRFRVIVLMIALITAWGIYSFT
                     KLPRESNPEVKIPIAVVSTVFPGAAPADIEELVTKKIETKIAGISGIDTVTSHSSNSI
                     SSVVVQFDADLDVDDSVRKVRDALGNIKNELPEDAGDPVVGEVSLDDTPILSVALSGD
                     YNGFTLYDYAKDIKDELEKIPGVREVSIAGGDRREIEINYSPEKLNYYGITLPQANNA
                     VAAANISVPSGNFEGSVYVYPVRTDGRIYDARQLQELPVGTAGGTPVFLKDVAVVEET
                     SIEKTKISRIASNGQSPSDAVTLSIVKRTGGNIIDTVAQAKNVLNDFTREIPGLKYDT
                     TYDQAKYIQEDFDQLTHDFVLTLFLVMGVLFLLIGLKEALVAGLAIPLVFFITFGIMD
                     AAGITLNFLSLFSLLLSLGLIVDDAIVVVSATKQYMRTGKFTPEEAVLLVLNDFKVVL
                     TTTTLTTVWAFLPLLFSSGIMGQFLKSVPITISVTLLASLAIALMVNHPLAAILERVR
                     LTKNFFFLYCAALAGLAAILLFQNNVFAQFGGAIILLAAGLMVYWYEKGGKNALENNQ
                     RLMRHEAEDDEAVKQKMRGLETRENAGLSHRLLHGIIDLGATLPLYEKYLSPLIDSKK
                     SRRRFFGGIVALFVAATALPAAGILKSEFFPADDFGYMYINIETPTGYRLAQTDAITE
                     KIEEKLLQYEEIDNFSTTVGGAVSLYSTGGSGGGSSNKASITINLVEKNRRELKSYEL
                     EEILRREFAGVPDAKVTVVSLRGGPPAGSAFQAEISGSDLDELKKIADDLQPALQSIP
                     EVVNVSTSLKESVPQYTFNLDHAKLAQNNLTAAYVGSVLRAAISGVETTSILRDNEDV
                     KVIARFDPASIPDLAAIQNLQIANGGGKSVYLKDVSKISLDPSVESIMRVDQKRTVNL
                     TGDITAKTTSDTVLSEFKKKTASYDLPEGYEISYGGANETNAESVQSIIAAMGVAFIL
                     IVATMVIQFNSFIKAAIVLITMPLALIGVFFGLTLFGIPLSFPGLIGILALFGIVVKN
                     AIILIDKISLNLNNGIAFRDAIIDAGKSRFEAIFITSFCTIIGIIPITLSSTTWQALG
                     AAIICGLSVSSFFTLFMVPALFAAFFNPEKNAPA"
     gene            complement(5041..5319)
                     /gene="nrdD"
                     /locus_tag="PKK37_01980"
     CDS             complement(5041..5319)
                     /gene="nrdD"
                     /locus_tag="PKK37_01980"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNR81182.1"
                     /translation="MDPEKKCHDCQALIELDGNEIKNGVLLAYKDEDGKEYEVFKCRA
                     CYDASPRLADYRPCEVYSRVCGYLRPVGQWNAGKRQEYAERKEYKSEC"
     gene            complement(5864..6070)
                     /locus_tag="PKK37_01985"
     CDS             complement(5864..6070)
                     /locus_tag="PKK37_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81183.1"
                     /translation="MGNSSALPEYDSQRDITTNTVKNISLSETKYVEFELPAGRYWLW
                     SSSGGDIVIYSCEEGGVSDPKPVW"
     gene            complement(6172..6369)
                     /locus_tag="PKK37_01990"
     CDS             complement(6172..6369)
                     /locus_tag="PKK37_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81184.1"
                     /translation="MEISQIAKESLFFIKTVGLMGSLLLLLFIKTEKTILKIVMIVVL
                     SILLLATILYSLSFASFVNLP"
BASE COUNT         1694 a         1697 c         1487 g         1548 t
ORIGIN      
        1 tatgatttcc atcaaaaagc ataagtttgg attcctgttc gccgttcttg tcattgccct
       61 ggtatcggcg gcaatcggca accaaatgaa aaacggcgcg cagagccaaa ccgaagaatc
      121 aatccccgcg gtgaagctaa tcgccgtcgg agattttcgg gaacaaaaaa ccgtgcgggt
      181 ggaaggcggc accgtccaat cgcttgatca ggcggatttg aaaagccaaa tctccgcgca
      241 aatcaccgcg attaacgtca aactcggcga tcaagttcag gccggacaaa tcatcatgca
      301 attacaaaac agcgacataa gcgcgcagtt ggagcaggcg cgggcgcggc ttgacgaatt
      361 gaaaaaaggc gcgcgcgacg aagaattgaa tcttgcccga accgccaaca acgaagccaa
      421 agccgcgttg gtgaattcca taaaagatgc ctacgcgaaa tccgatgacg cgatccacaa
      481 tcatattgat aagtttttca ccaacccgcg cgggaaaaat atccgatttt ccatcattct
      541 gaacgtcggc ggaagccagg tgagtttcta tccttcggat tcggatttgg gacagcgcgt
      601 tgcggataga aaatataaaa ttgaagcgat gcttgacgaa tggaaagatc tgctggccgg
      661 cgccgacgcg gcggcggatg acgccgacat tgaaaacatc ttggcggttt ccaaaaaaaa
      721 tctgcaggaa gagattgatt tcgccaatga aatggcgccg ctggtaaaca gtctttccag
      781 cgacaattca acctataaaa ccatcattga cggctataaa accgaatttt cggccgcgcg
      841 cagcgccatc aacggatcgc tggcatccct gcaaggcgcg cttaccgccc gggaaaccgc
      901 ccgacagact ttggaattga agctcgccgg cgcgagcccc gagcaactgc ggcaagccca
      961 agccgccgtt gacgcgcttg aatcaacgct ggctaaaact tcaatcgttt cccccatttc
     1021 ggggcaaatt tcttacatca acgggcgcgt cggcgagttg gccggcggcg gcagtttggt
     1081 cgccggcgtg gttaatcccg gcgccctgca aataaaagcc tatgtttccg aaaacgattt
     1141 gcgcgagatt gcggaaggcg cggcggtaaa aattgacggc ggcggcacgg gaattttgca
     1201 aacaatttcg ccggcggcgg acagccaaac aaaaaaatcc gaaatcaatg ttctgatcgc
     1261 aaaacccgga aacgcaccga tcatcgtggg acaaaccgtg ggatttgaaa tcgccgccaa
     1321 aggcggcgcc gatccaaacg cctatctttt gccgattgag gcgatccaat tcactgccaa
     1381 cggcaattat gttttgacag tcggtgccga aagcgcgctg gaacgaattc cggtaaccac
     1441 cggcgacatt gtgggtgaaa aaatctatgt tgtccaaggt cttgacggcg atgccaaaat
     1501 catctcatcc actcgcggat tgaaaagcgg cgacatcatc aaagttgaaa attnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaactct atctgaaccg gctgaaattc
     1681 aaaccggagc tgaaaaaaat gtggctcagt ttttttgtct accgctttcg ggtgattgtt
     1741 ttgatgatcg cgctaatcac cgcctgggga atttattcgt ttacgaaact gccgcgcgaa
     1801 tccaaccccg aagtcaaaat cccgattgcc gtggtaagca cggtttttcc cggcgccgcg
     1861 ccggccgata ttgaagaatt ggtaaccaaa aaaatagaaa ccaaaatcgc gggaatttcg
     1921 ggcattgaca ctgtcacttc tcattcgtca aattcaattt ccagcgtcgt ggtgcagttt
     1981 gacgccgatc ttgacgttga cgattccgtc cgcaaggttc gcgacgcgtt gggtaacatt
     2041 aaaaacgaac tgcccgaaga cgcgggcgat ccggtggtgg gcgaagtatc gctggacgac
     2101 acgccgatac tcagcgtcgc cttgagcggc gattataacg gcttcacgct atatgattac
     2161 gccaaagaca taaaagacga actggaaaaa atccccggcg tgcgcgaagt gagcattgcc
     2221 ggcggcgacc ggcgggaaat tgaaattaat tattctcccg aaaaattaaa ctactacggc
     2281 atcaccctgc ctcaagcaaa taacgccgtc gccgcggcca acatcagcgt tccttcgggt
     2341 aattttgaag gttcggttta tgtttatccg gtgcgcaccg acggccggat atacgacgcc
     2401 cggcaattgc aggaattgcc cgttggcacg gccggcggca caccggtttt tttgaaagat
     2461 gtcgcggtgg tggaagaaac ctccattgaa aaaacgaaaa tctcgcgcat tgcttcaaac
     2521 gggcagtcgc cgagcgacgc ggtaaccctg agcatcgtca agcgcaccgg cggcaacatc
     2581 attgacaccg tcgctcaagc aaaaaacgtt ttaaacgatt tcacccggga aattcccggt
     2641 ttgaaatatg acaccaccta cgatcaggca aaatacattc aggaagactt tgaccaattg
     2701 acgcacgatt ttgttttgac gctgtttttg gtgatgggcg tgcttttttt gctgatcgga
     2761 ttgaaagaag ccttggtcgc gggattggcg attccgctgg tgtttttcat caccttcggc
     2821 ataatggacg ccgctggaat caccctcaac ttcctgtcgc tgttttccct gcttttgtcg
     2881 ctggggctga tcgttgacga cgccattgtc gtggtctcgg ccaccaaaca atatatgcgc
     2941 accggcaaat tcacgcccga agaagcggtg cttctggtgt taaacgattt caaggtggta
     3001 cttacaacca cgaccctcac caccgtctgg gcatttttgc cgctcttgtt ctcttcggga
     3061 attatgggac agtttctgaa atccgtgccc atcacgattt cggtaaccct gctggcaagc
     3121 ctggcgatcg cgcttatggt caaccacccg ctggcggcga ttctggagcg cgtccgcctg
     3181 acaaaaaatt tctttttcct ttattgcgcg gcgctggccg gattggcggc aatcctgctt
     3241 ttccaaaaca acgtcttcgc gcaattcggc ggcgcgataa tccttcttgc cgccggattg
     3301 atggtctatt ggtatgaaaa aggcggcaag aacgcgctgg aaaacaacca gcggctgatg
     3361 cggcacgagg ctgaagacga cgaagcggtc aaacaaaaaa tgcgcggctt ggaaacacgg
     3421 gaaaacgccg gcctttcgca ccgcctgctc cacggcatca ttgacttggg cgccaccctt
     3481 cctctctatg aaaaatatct ttcccccttg attgacagca aaaaatcgcg gcggcggttt
     3541 tttggcggca tcgtcgcgct ttttgtcgcc gcaacggctc tgcccgccgc cggaatttta
     3601 aaatccgaat tttttccggc cgatgatttc ggctatatgt acattaacat tgaaactccg
     3661 accggctacc ggctggccca aaccgacgca atcaccgaaa aaattgaaga aaagcttttg
     3721 caatacgaag aaattgacaa tttctccacg actgtcggtg gcgcggtttc tctttattcc
     3781 accggcggat cgggcggcgg cagttcaaac aaggcttcca tcaccatcaa tctggttgaa
     3841 aaaaaccggc gcgaattaaa atcatatgag ctggaagaaa tcttgcgccg cgaatttgcc
     3901 ggcgttccgg atgccaaagt caccgtggtt tccctgcgcg gcggcccgcc ggccggctcc
     3961 gcctttcagg cggaaatttc cggatcggat ttggatgaat tgaaaaaaat cgccgatgac
     4021 ctgcaacccg cgctccaatc aattccggaa gtcgtcaatg tttcaacttc gctcaaagag
     4081 tcggtgccgc aatacacttt caatcttgac catgccaaac tggcgcaaaa caatttgact
     4141 gccgcttatg tcggttcggt tttgcgcgcc gcgatttccg gcgttgaaac cacctcaatc
     4201 ctgcgcgaca acgaagacgt taaagtaatc gcacgatttg accccgcgtc cattcccgat
     4261 ttggcggcga tacaaaatct ccagatcgcc aacggcggcg gcaagtcggt ttatctaaaa
     4321 gacgtttcaa aaatatcgct ggacccttcg gtggaaagca taatgcgcgt tgatcaaaag
     4381 cgcaccgtca atttgaccgg cgacatcacc gccaaaacca cctccgacac agtgctgtcc
     4441 gaattcaaaa agaaaaccgc gtcatacgat cttcccgaag gctatgaaat atcctacggc
     4501 ggcgccaatg agaccaacgc cgaatcggtg caatcaatca tcgcggcgat gggagttgca
     4561 tttattctga tcgtggccac gatggtgatt caattcaact catttatcaa ggccgcaatc
     4621 gtgctgatca cgatgccgct ggcgctgatc ggcgtgttct tcggactcac gttgtttggt
     4681 atcccgttga gtttcccggg cttgatcggc attctggcgc tgttcggcat cgtggtgaaa
     4741 aacgccatca tcctgattga caaaatcagc ctgaatctca acaacggcat tgcttttcgc
     4801 gatgcaatca ttgacgcggg aaaatcccgt tttgaagcga ttttcatcac ttcgttttgc
     4861 accatcatcg gcatcatccc gatcacgctt tcgtcaacga catggcaagc gctgggcgcg
     4921 gccattatct gcggcctgtc ggtatcgtcg ttttttaccc tttttatggt gcccgcgctt
     4981 tttgccgctt tcttcaatcc cgaaaaaaac gcgccggctt gaccggcgcg ttttgaattt
     5041 tcagcactcg cttttgtatt ctttcctttc ggcatactcc tgccgtttgc ccgcgttcca
     5101 ttgccccacc ggccgcagat agccgcagac ccgcgaataa acctcgcatg gccggtaatc
     5161 cgccagccgc gggctggcat cgtagcaggc ccgacatttg aaaacttcat actccttgcc
     5221 gtcttcgtct ttataggcca gcaagacgcc gtttttgatc tcgttgccgt caagttcaat
     5281 caacgcctga caatcatggc actttttttc aggatccata attttttaaa acccaaatgc
     5341 caaagttcaa acgccaaaaa aaattcaagg ctcaaatttc aaatatcaaa tcgcaaatta
     5401 tcttgaaatt ttgattttgg atttattttg acatttgtca tttggatttt gacattaact
     5461 ttttgtgttt tcattctatc cgacccaaat tcgccgtcaa gtccatgcat tttccccacc
     5521 aaaacaccgc gccaaattat aaaataaaaa ggaggagtcg ctaaaaaaat cagcgaactc
     5581 ctcatccatc taccgacgcg gatattgcgt cgggatatcc gtgccggaca gctgtttttc
     5641 ccgaacaaca gtttcgggaa aaacagcaag aacaagtagc aacatatcag agcgatgaaa
     5701 agcctgatgc attgttcccc aaaaattcac tctctcacct ccttttaatc actccctcct
     5761 taggagcgct atggtgccat tatacaataa atatcaaaaa aatcaactct ctgtatgtat
     5821 gcaattttca gagggtctaa ccctctgaaa accctccgca aaactaccac accggcttgg
     5881 gatcgctcac cccgccctcc tcgcagctgt aaatcacaat gtccccgccg gagcttgacc
     5941 aaagccaata cctccccgcc ggcaattcaa actcaacgta tttcgtttca gaaagactga
     6001 tattttttac cgtattcgtc gtaatatctc tctgcgaatc atattcgggc aaagcggagc
     6061 tattgcccat aaaaatagcc gtggagccaa gcgacgatcc tgaaaatccg ctgtgcggaa
     6121 accgtttggc agtaatatat agcttgcctc catttgtggt aaattcagct tttatggcaa
     6181 attaacaaaa ctggcaaagg aaagactata taatattgtt gctaacaata agatcgataa
     6241 aacaacaatc atcacaattt ttaatattgt tttttcagtt ttgataaaaa gcaataataa
     6301 taaacttccc atgagaccga ctgtcttgat gaaaaataat gattctttag caatttgcga
     6361 aatttccaaa ttgaataaat caaattttaa tgattccccg agtttaaaac ttattccaaa
     6421 aaaaatctct cttatccaat aaaataatat tacggttacc tcgggattga taatctccgg
     6481 caccagaaac atcgccaaaa aaaccgccag ccatattttt tgcttt
//
LOCUS       DAOQWE010000035         6511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_12237_length_6511_cov_7.838910, whole genome shotgun sequence.
ACCESSION   DAOQWE010000035 DAOQWE010000000
VERSION     DAOQWE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12237_length_6511_cov_7.838910"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..75)
                     /locus_tag="PKK37_01995"
     tRNA            complement(2..75)
                     /locus_tag="PKK37_01995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Val,seq:tac)
     gene            complement(126..207)
                     /locus_tag="PKK37_02000"
     tRNA            complement(126..207)
                     /locus_tag="PKK37_02000"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Leu,seq:gag)
     gene            complement(198..842)
                     /locus_tag="PKK37_02005"
     CDS             complement(198..842)
                     /locus_tag="PKK37_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81185.1"
                     /translation="MNHDIAAIKWSPQIAYAVGLIVTDGNLSSDGRHFDFTSKDVELV
                     KTFKKCLNINNKISLKSAGKSNKKYHRVQFGNICFYRQLLNIGIAPNKTKTIGILQIP
                     DEFFFDFLRGHFDGDGSCYGYWDKRWKNSFMFYVKFVSASKSHISWLQAKIRDALGIN
                     GAIGKAGSIYQLSYAKKESKVLSTKMYYDREIPCLKRKFEKLQKLLEIDKNPGE"
     gene            complement(866..1279)
                     /gene="rplS"
                     /locus_tag="PKK37_02010"
     CDS             complement(866..1279)
                     /gene="rplS"
                     /locus_tag="PKK37_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNR81186.1"
                     /translation="MEKQIKKFAAQIKRQDIPDIKTGDTVRVHQKYTDKGKEKIQIFE
                     GLVLCRKHGKETGAAITVRKIASGVGVEKIFPVHSPMIAKIEVVKSGKVRRAKIYYIR
                     NAKGRKSRLKRTERKIKTAPESNSKPEIETQNQEA"
     gene            complement(1364..1435)
                     /locus_tag="PKK37_02015"
     tRNA            complement(1364..1435)
                     /locus_tag="PKK37_02015"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1401..1403),aa:Val,seq:gac)
     gene            complement(1454..1639)
                     /locus_tag="PKK37_02020"
     tRNA            complement(join(1454..1490,1602..1639))
                     /locus_tag="PKK37_02020"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1603..1605),aa:Ile,seq:tat)
     assembly_gap    1494..1593
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1779..3605)
                     /gene="ftsH"
                     /locus_tag="PKK37_02025"
     CDS             complement(1779..3605)
                     /gene="ftsH"
                     /locus_tag="PKK37_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNR81187.1"
                     /translation="MKGFLKNFVFAVLMLAATAGFFSLFLRESEEPKEVSLSQIVADI
                     NRENIKTIAVAGNSLEIVYQNGEKATAKKEADSSISQTLANLGAQNEKMGLVDFSAKN
                     DDSVWVWLGPLLYSVLPLIVILFVFAMIFRQAKGNAMQAFDFTKAKTRILGGDNQKEK
                     TGFGDVAGLKEAKEELTEIIDFLKNPEKYLKMGAKIPRGVLLMGLPGTGKTLLAKAVA
                     SESNVPFFSISGSEFIELFVGVGASRVRSLFEQAHKAKRAIIFIDEIDSIGKTRGVGV
                     TGGHEEREQTLNQILAEMDGIESGEGIIVLGATNRPEHLDPALLRPGRFDRRIVLDLP
                     DANDREAILAIHCRKKPLAADVNLREVAQRTPGFSGADIANVVNEAALLAARREKTQI
                     FQSEFLESIEKVLLGPEKKSHLLSKEEKKIAAYHESGHALVSSSLPESEPVRKISIIS
                     RGMAAGYTLKTASEEKKLRTKNEFVAEIATLLGGMIAEALVFGQSSTGASNDLKVASK
                     IARKMVKEYGMSPLGPIVFGEKEELAFLGQEFESARNYSEEIAAKIDAEVAKIIKEGQ
                     EKARQILSEKRNLLEKIAVRLIEKETIEKEEYEELIGIAAKA"
     gene            complement(3689..4537)
                     /locus_tag="PKK37_02030"
     CDS             complement(3689..4537)
                     /locus_tag="PKK37_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNR81188.1"
                     /translation="MNNANKETAKGDNAGIAAINPPRTGEIVKGKIISQERSALYVDL
                     GAVGTGIVYGKEFYEVKDRLKDTQEGDPISAKIVSLENEDGYIELSVSGATRKLVWDE
                     LREKQENREAVQIKVAGANKGGLMAKVSGLPAFLPVSQLASHHYPKVVGGDPDKILKE
                     LQKFVGEDLSVRILNLKPKTGEIILSEKMAEMEQREERLKNYNAGQEIGGTITGIMEF
                     GIFIRFDNDTVEGLIPAAQIPEELKKSLSENFKIGGKLSARIVEISNGKVFLSLKDPA
                     QQSQTA"
     gene            5174..5764
                     /locus_tag="PKK37_02035"
     CDS             5174..5764
                     /locus_tag="PKK37_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR81189.1"
                     /translation="MSKKEQIWREILQKAITHRQFEFTQKDIADNLKVSLSTVFNALK
                     VLRAQGAVKVGGRNFLVVDIEKLLYIWATQRKMEKYIIYRAAVSASAKEIEGMMPDGA
                     IFGAYSAYSQKYNDAPADYDKVYVYADAPVLAEIKKRFPRRTGEPNLFVLKSDRRLKA
                     FGLIAPESQIFCDLWNLKDWYAKDFLKSLKERIISR"
     gene            5766..6377
                     /locus_tag="PKK37_02040"
     CDS             5766..6377
                     /locus_tag="PKK37_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81190.1"
                     /translation="MQFYHEIITQKSFVFLQGLKKEYEFVLIGGWAVFLYSHGLKSKD
                     IDIIVGYSELAKMKEKYEVLKNERLAKYEIKTGEFDIDIYLSHYSDLGVDIVGIEESA
                     VIKDGFLVPKLELLFLLKLFAWDNRRGTAKGRKDELDVFCLARLPEFDWKYYSQMVKK
                     YSFEERHRLFIKLLKTTKEIKELAINQQKMAKLKKDILGKLPK"
BASE COUNT         1557 a         1492 c         1517 g         1845 t
ORIGIN      
        1 gtgggcgatg agggattcga acccccggcc tactcggtgt aaacgagtcg ctccggccaa
       61 ctgagctaat cgcccgaatc cctattaact attaacgaaa aattttaaca aagttaaaat
      121 ttttgtgggc gaggagggac ttgaaccctc acgcctttcg gcctacgcac ctcaagcgta
      181 gatgtctgcc ggttccatca ctcgcccgga tttttgtcga tttctaataa tttttgcaat
      241 ttttcaaatt tacgcttcaa acatggtatt tctctatcat aatacatttt agttgataat
      301 accttcgact cttttttcgc ataacttaat tggtatatag accccgcttt tcctatcgct
      361 ccgttaatac ctaatgcatc tctgattttt gcctgaagcc aagaaatgtg gcttttgctg
      421 gcggaaacaa atttcacata aaacataaaa ctgtttttcc atcgtttatc ccaatagcca
      481 tagcaacttc cgtctccatc aaaatgacct cgtagaaaat caaagaaaaa ttcatccgga
      541 atttgcaaaa ttcctattgt ttttgttttg tttggtgcga ttccgatgtt taataattgg
      601 cgataaaaac agatgttccc gaattggact cggtgatatt ttttattgga cttaccggca
      661 gatttcaggc taattttatt attgatattt aagcatttct tgaatgtttt tactaattca
      721 acatccttgg aggtaaagtc aaaatgcctg ccatctgatg acaaattgcc atcggtaaca
      781 attaaaccca ccgcgtaagc gatttgcgga gaccatttta tcgcagcgat gtcgtggttc
      841 atgttatttt taaatcaaga agcggttagg cttcttgatt ttgggtttct atttcgggtt
      901 ttgagtttga ttcgggcgcg gtttttattt ttctttccgt tcgcttcaat ctggacttgc
      961 gtcctttggc gttgcggata taatagattt ttgcccgccg cacttttccg gatttgacca
     1021 cttcaatctt ggcgatcatc ggggaatgaa cggggaaaat cttttccacg cccacgccgg
     1081 aagcgatttt gcgcaccgtg atggccgctc ccgtttcttt gccatgtttg cggcacaaca
     1141 ccagcccttc aaaaatctgg attttttcct tgcccttgtc ggtgtatttt tgatgcaccc
     1201 gcacggtgtc gcccgttttg atatcgggaa tgtcttggcg cttgatttgc gcggcgaact
     1261 ttttgatttg cttttccata atgatatttc aatttgtatt ggatttgcca acaactatgg
     1321 actataaact aaaaaccgtt tttttgcaac aggcggacgc gggtgggcga tacagggatt
     1381 gaacctgtgg ccttttcgat gtcaacgaaa cgctctacca ctgagctaat cgcccgaaat
     1441 ttactgtgtc ttgtgggcgt gggtgggctc gaaccaccga acctcgtctt tatnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnactaccg tcataagaga cgcgctctca
     1621 ccgactgagc tacacgccca aaagaattct agttactacc gtcataagag ccgtgctcta
     1681 actacctgag ctacgcgccc aaacaaatta ttacatttcc ggtataagga actgtgctct
     1741 accactgagc tacgcgcgta tagtgccggc agattatgct acgctttcgc ggcgataccg
     1801 attaactctt catactcttc cttttcaatg gtttcttttt ctatcaggcg gacggcgatt
     1861 ttttccagca agttgcgttt ttccgaaagg atttggcgcg ctttttcttg cccctccttg
     1921 atgatttttg ccacctcggc gtcaattttg gccgcgatct cttcggaata gttgcgggcg
     1981 gattcaaact cctgtcccaa aaacgccagt tcttcttttt cgccaaacac gatcggcccc
     2041 agcggcgaca ttccatattc cttcaccatt ttgcgcgcga tcttggatgc cactttcaaa
     2101 tcattggacg cgccggtaga cgactggccg aaaaccaacg cttccgcgat cattcctccc
     2161 agcaaggtgg caatctcggc gacgaactcg tttttggtgc gcaatttttt ttcttcggag
     2221 gcggttttca gcgtgtagcc ggcggccatt ccccgggaga tgatggaaat tttccgcacc
     2281 ggttcggatt cgggcaagct tgaagaaacc agcgcgtgcc ccgattcgtg ataggcggca
     2341 atcttttttt cttctttgga caagagatga ctctttttct ccggcccgag caggactttt
     2401 tcaattgatt ccaaaaattc cgattggaat atctgtgttt tttcccgcct tgccgccaac
     2461 agcgcggctt cgttgaccac gttggcgatg tcggcgccgg aaaatcccgg cgtccgttga
     2521 gcgacttcgc gcaaattgac atccgccgcc agcggtttct tgcggcaatg aatggccaga
     2581 atcgcttcgc gatcgttggc gtcgggcaga tccaaaacga tgcgccggtc aaatcggccg
     2641 gggcgcaaca gcgccggatc caaatgttcc ggccgattgg tggcgcccag aacgatgatg
     2701 ccttcgccgc tctcaatgcc gtccatttcc gccaggatct ggttcaacgt ctgttcgcgc
     2761 tcctcgtgtc cgccggtgac tcccacgccg cgagttttgc cgatggagtc aatttcgtca
     2821 atgaaaatga tcgccctctt ggccttgtgc gcctgttcaa acagactgcg cacgcggctg
     2881 gcgcccacgc ccacgaaaag ctcaataaac tccgatcccg aaatggagaa gaacggcaca
     2941 ttgctttcgc tggcgaccgc tttggccagc agagttttgc cggtgccggg aagccccatc
     3001 agcaagacgc cacggggaat cttggcgccc attttcaaat atttttccgg atttttcaga
     3061 aaatcaatga tttccgtcag ttcttctttg gcctctttta atccggcgac gtcgccaaaa
     3121 ccggtttttt ccttttgatt atcgccgccc aaaatccgcg tcttggcttt ggtgaaatca
     3181 aacgcctgca tcgcgttgcc ttttgcttgg cggaaaatca tcgcgaaaac aaacagaatg
     3241 acgatgagtg gcagaacgct gtaaagcagg ggtcccagcc aaacccatac gctgtcgtca
     3301 tttttggcgg aaaaatccac gagccccatt ttttcattct gtgcccccag attggccaag
     3361 gtttgggaga ttgaggagtc ggcctctttt ttcgccgttg ctttttcgcc gttttgataa
     3421 acgatttcca gggaattgcc ggcgaccgca atggttttga tgttctcccg gttgatgtcg
     3481 gcaacgatct gcgagagaga aacttccttc ggttcttcgg attcacgcag gaatagcgaa
     3541 aagaaaccgg cggtggcggc cagcatcagc acggcgaaaa cgaaattttt caaaaaacct
     3601 ttcattggat attataagag tccaaagtca aaagacgggc gccggttgct gtcgcggcgt
     3661 tttggagcgg gatattcatc aaaacgtttc aagcggtttg gctctgttgg gcggggtctt
     3721 taagagataa aaataccttg ccgttgctga tttcaacaat acgggcggaa agcttgccgc
     3781 cgatcttgaa attttccgac aaacttttct tcaattcttc gggaatttgc gcggcgggga
     3841 tcagtccctc cacggtgtcg ttgtcaaaac ggataaaaat gccgaattcc ataatgccgg
     3901 tgatcgtgcc gccaatctcc tggccggcat tgtaattttt cagccgctct tcgcgctgtt
     3961 ccatttccgc cattttctcc gaaagaatga tctcgccggt tttcggtttc aggttcaaaa
     4021 tccgcacgga aagatcttcg ccgacgaatt tttgcaattc tttgagaatt ttgtccggat
     4081 cgccgccgac aaccttggga taatgatggc tggccagctg ggataccggt aaaaaggcgg
     4141 gcaatccgga aactttggcc atcaggccgc ctttgttggc gccggcgact ttaatttgca
     4201 ccgcttcgcg gttttcctgc ttttcgcgca attcgtccca caccagtttg cgcgtcgcgc
     4261 cgctcactga cagctcgata taaccgtctt cattttccag actgacgatt ttggccgaaa
     4321 tcggatcgcc ttcctgagtg tctttgaggc gatctttgac ttcataaaat tctttgccgt
     4381 agacgatacc cgtcccgaca gcgcccaagt ccacataaag cgcggatctt tcctgggaaa
     4441 tgattttccc cttgacgatt tccccggtgc gcgggggatt gattgcggcg atgccggcat
     4501 tgtcgccctt cgccgtttct ttatttgcgt tgttcataat tgccacttta caacattttc
     4561 ctttgttttt caagtttttt tgccggcaaa cattgacaac cgctatggtt gcgttttatt
     4621 atgaggacag cttgcgggga atcggaaacg cgtttcccga gagctgagtt tgcgcgaagg
     4681 gcgcatctcc cgctttcagc gggagataaa aaaacaaaaa ttgaagtttt tgcgccaaga
     4741 ccgcccattt ttggcggaaa aacaaaagtt tttgttttca aaaaatgatt tacgacgaca
     4801 acggcactac cgccccggca gaaccggcga caactccgga accggcgaca acctcgccgg
     4861 ccgaagaaac tccggccgcc taaaactcaa gcgcgggaat gcggcatgcg cgaagcattc
     4921 ccgaaaggac tggtataaat ccggtcgttt tgttccggat cgccgattgc gcgcggctgg
     4981 tatgggcgcg gcgcgcgcgg gaaaactggc gatcaacaga aacggacgca agtccgtttt
     5041 ttgttcgccc ctccggtccg atatttttta tttttggatt taattttttc ggtgcgagtg
     5101 caaagatttg acggctgatt tcgtttctat tatcatagaa atgaggcgat aatttaggct
     5161 ggaaaatcga aatatgagca aaaaagagca aatttggcgg gagattttac aaaaggcgat
     5221 tacccatcgc cagtttgaat tcactcaaaa agatattgcc gataatttaa aagtgtcatt
     5281 gagcacggtt tttaacgctc tcaaagttct tcgtgcccag ggcgcggtta aagttggcgg
     5341 tcgcaatttt ttggtggttg atattgaaaa gcttttatat atttgggcga cgcaaagaaa
     5401 aatggaaaag tatataatct atcgcgccgc ggtttcggca agcgccaaag aaattgaggg
     5461 gatgatgccc gacggggcga ttttcggcgc ttacagcgcc tactcgcaaa aatataacga
     5521 cgcgccggcg gattacgata aggtttatgt ttatgccgat gcgccggttt tggcggaaat
     5581 caaaaaaaga tttcccaggc ggacaggaga acctaatttg tttgtgttga aaagtgaccg
     5641 ccggctgaaa gcttttgggt tgattgctcc cgaatcgcag attttttgcg atctttggaa
     5701 tttgaaagat tggtatgcca aagatttttt aaaaagcctt aaagaaagaa taatttcccg
     5761 ataaaatgca attctatcac gagataatca cccaaaagag ttttgttttt ttgcaggggt
     5821 taaaaaaaga gtatgagttt gttcttatcg gcggctgggc ggtgtttttg tattctcacg
     5881 gtttgaaatc aaaagatatt gatattattg tcggctattc cgaattggca aaaatgaaag
     5941 aaaaatatga agttttgaaa aatgagcggc tggcaaaata tgaaattaaa accggcgagt
     6001 ttgatattga tatttatttg agccattatt ccgatttggg tgttgatatt gttggaattg
     6061 aggagagcgc cgttatcaaa gacggatttt tggttccaaa actggaattg ctgtttttgc
     6121 tcaaattgtt tgcttgggat aatcgccgcg ggacggcgaa aggccgaaaa gatgagttgg
     6181 atgttttttg tttggcgcgt ttgcccgaat ttgattggaa atattatagc caaatggtca
     6241 agaagtattc atttgaagaa cggcaccgac tatttatcaa gcttctcaaa acaacgaaag
     6301 aaattaaaga attggcgatc aatcaacaaa aaatggccaa attgaaaaaa gatattttgg
     6361 ggaaattacc aaaatagaca ttaatttagc acaaggggga aaattacggg ggttggcccc
     6421 ctgtaatttt ttgcccgggg caggacttta aatattggcc agatttgcta cattgaagtt
     6481 gtggcaaaaa acaatatgga gcaaaattat a
//
LOCUS       DAOQWE010000036         6377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_12555_length_6377_cov_6.419329, whole genome shotgun sequence.
ACCESSION   DAOQWE010000036 DAOQWE010000000
VERSION     DAOQWE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12555_length_6377_cov_6.419329"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKK37_02045"
     CDS             <1..184
                     /locus_tag="PKK37_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81191.1"
                     /translation="RADGGAEIRKEENKAVKTLAPPSRARVFDILLPFSFTIPKYFGN
                     IMLWERSICINKYTTI"
     gene            200..412
                     /locus_tag="PKK37_02050"
     CDS             200..412
                     /locus_tag="PKK37_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81192.1"
                     /translation="MAESSKNNLSKTVKRLREKMGISQEKLARLADVSNNTIINIEAG
                     KQGNPTIETLKKIAKALNTPIEDLIK"
     gene            415..1644
                     /locus_tag="PKK37_02055"
     CDS             415..1644
                     /locus_tag="PKK37_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81193.1"
                     /translation="MKNHIPVLEKLFDKNYQLVDGTDEIFELDLALWEYEVLPKKELV
                     ERSAYFKLIDGKETAHYKTCNLQNLEEVHKNSSFRTKIFFLDGKYSTGYATHGLFPYR
                     GKFHPQLIRALLNILDIKPGHVVLDPMAGSSTVAVEANLLGIDAISVDLSPFCELMGK
                     VKTFALNLDIKILESIIKNPKEILDKLKKDKVPEYFKNNKDDKKRNYYEIILLAFLDT
                     VGFAGRSSSSVDKLFPRVLDRYVSTIKYFQEARQKMDLKIGKSKIIQGSVLNLPLGDN
                     SVDAVITSPPYSFAIDYLKNDQPQLEYLGHDLEVLRQEMIGLQGRGVENKLEIYFDKM
                     NQALGEMKRVSKKNAPIVIIIGTNDIQTNGVRLETKVIELGEKQGLKFELNLKKPIRG
                     LQNTMKEESILFFTNQK"
     gene            1649..2638
                     /locus_tag="PKK37_02060"
     CDS             1649..2638
                     /locus_tag="PKK37_02060"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HNR81194.1"
                     /translation="MKYTKPTTELKFKTVINKNTFYFFNPAFEEKYEGYLNSLKEMLL
                     ILKNNIETQGLKKEFFEQLLIEKENGLRALLALTGFSNETLKRLTTIIRVVNDPELSK
                     LVYKDKWFKDEDINNLKEWSDAQIAKFIKENEFFRKGILNIFFEGSTVPFLTKTIPLF
                     ELKKLSISKLKFELPEMIDTLIRYKEKGSYSGMKENNPEILIEEILNHLEIPFEKGDL
                     SELIDNAPDNKRTMDFIIPNKQNPLIIAESSFLVTTSSGQGDKSKTEISIDSLIKSHY
                     PKAKFIGFVDGIGWYVRKGDLKRMVTAYEDVFTFHREELKRFEKILIETFLSK"
     gene            2683..3546
                     /locus_tag="PKK37_02065"
     CDS             2683..3546
                     /locus_tag="PKK37_02065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81195.1"
                     /translation="MEVLKKIPDNSVDMTFADPPFNLKKKYNHYEDSKEKQDYLNWCN
                     DWMSEMVRITKPTGAIFVHNIPRWLSHYAEHLNKIAYFRHWIAWDSGGAPMGKTLLPN
                     HYGILYYTKSKSHKDFKFYDIRYPHPRCRACDEFLKDYGGKKDQAHGFGPLLSDVWND
                     IHRIRHKKRRDEHPCQLPIPLLERLILMTTDEGDVVFDPFMGTGTTAVAAKQLGRKYI
                     GIELDSDYVKIAEKNIKATKEMKINGYFVSVYLGHIRTMRNKDFKEIWKNKGDLKLLD
                     KKKYKIVKTTE"
     gene            3554..6322
                     /locus_tag="PKK37_02070"
     CDS             3554..6322
                     /locus_tag="PKK37_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81196.1"
                     /translation="MANNLLSKGSVWRKWDLQVQTRLDANYTCLGNNSLSAADLQKLI
                     TATGLTETEITAQEKEIDAQKYAKLFIKYVEFFTDISVFGITDHNTGREIDALINEAK
                     NSSREIAILPGVEVSSAQGLHILCIFDPEKKWRDNWAESIDHFLTELGLTNGVFNTQN
                     QPLNSTKTAQEILTITNKKGGLCIFAHIGTDNGLFKFSNTASGGNAHISVYKDKLCQI
                     VQLPRSANLSNGVQNIIEGKDPQYGNKSVTQIKCSDARKLTEIGACFTWIKADQTFDG
                     LKQIIFEPEDRVKIQENSPYEDRKKIYFENLTIDGRINFILPNITIPLNSELITVIGG
                     RGSGKSALLEAFAFLNEEHLKVDQNGKKKIIEFYRDNEVATDPAPSFITKTTLVDKDL
                     NKQEFQKQLAEHENLELPFLYLGQEQLSGLATNDIELTKTICELIGIDIAELSQQAHI
                     FKAREILAQIKNTNQSLGDVFNIYKQIGYNETTDFEKWIASYLKKLGAQQKRLTSKET
                     RTTLEEINKNTQRGLKLKELLEQIDETAKELNTLVVNTSINSLNKRLAGLYPDEKPTI
                     TPIDSKKQLVEISQIQGQIKTEMDTLRKDIVGKKAELSKLGIKEDVNTLLQSSQTLQQ
                     QINNANKDLENYKNAIIRLSEFIKQRNDILVNVEGGLNELKEQIDVKFAEFKSSRNDS
                     EAQEKELFEKIIQGIEIEGDIVFDEEQFCNDVLGSFVDNRKIKNENDLREEIAGKNTD
                     GTAKHITLISIANWVKQDLSTKLYFNRSGVDGMLEYIFTNWPQFMRVRAIVKLNGKST
                     EVLSIGQRGTLLLKVFLATATAKQVFIIDQPEDNLDNNFIMHELVPLIRKAKVSRQII
                     MSTHNANLVVNADAEQIIVARLDNEGDYISGSIENQTINQSIKEILEGGEEAFRNREN
                     KYGMAIIN"
BASE COUNT         2377 a         1011 c         1221 g         1768 t
ORIGIN      
        1 gcgagcggac ggcggggccg aaatacgaaa agaagaaaat aaagcggtaa aaacgcttgc
       61 cccaccctcc cgtgcgcgcg tttttgacat tctactccct ttttccttta cgattcccaa
      121 atattttggt aatattatgt tgtgggaaag aagcatttgc ataaataagt ataccacaat
      181 ttaatattac taataaatta tggcagagtc aagtaaaaac aacctatcaa agaccgtaaa
      241 gcggttaaga gaaaaaatgg gaatttccca agagaaattg gcgcgccttg ccgatgtttc
      301 caataacacg attatcaaca tagaagcagg aaaacaaggc aatccgacta ttgaaacttt
      361 gaaaaagatc gccaaggcgt tgaatacacc aattgaggat ttaataaagt agctatgaaa
      421 aatcatattc ccgtattaga aaaattattt gataaaaatt atcagctcgt tgatgggact
      481 gatgaaattt ttgaattaga tttagctttg tgggaatatg aggttttgcc aaagaaagaa
      541 ttagttgaaa gaagtgcata ttttaaattg attgacggaa aagaaacagc tcattataaa
      601 acttgtaatc ttcaaaattt ggaagaagtc cataaaaata gttcttttcg gacaaaaatt
      661 ttctttttag acggaaaata ttcaacggga tatgccactc atggcctttt cccctatcgt
      721 ggcaaatttc acccgcaatt gataagagct ttgttaaata tacttgacat aaaacccggc
      781 catgttgttc ttgatccaat ggccggaagt tccactgttg cggttgaagc taatttgctg
      841 ggcattgacg ctatctctgt tgatttgagc ccattttgtg aactaatggg aaaagttaaa
      901 acatttgctt taaacttgga tattaaaatc ttagagtcaa ttatcaaaaa tccaaaagaa
      961 attttggata aattgaaaaa agataaagtt ccggaatatt ttaaaaacaa caaagacgac
     1021 aaaaaaagaa actattacga aataatttta ttagcgtttt tagacactgt cggatttgct
     1081 ggccgaagca gttcttctgt tgataaactt tttccgcgcg tattggacag atatgtttca
     1141 acgataaaat attttcaaga agcaaggcaa aaaatggatt taaaaattgg aaaaagtaaa
     1201 attattcaag gtagcgttct aaatttgcca cttggtgata atagtgtgga tgcggtaatc
     1261 acctcgccgc cctattcttt tgctattgat tatttgaaaa atgatcagcc ccaattagaa
     1321 tatctcggcc acgatctgga agttttgcga caagaaatga ttggcctaca agggcgaggc
     1381 gtagaaaata aattggaaat ttattttgac aaaatgaatc aggctcttgg cgaaatgaaa
     1441 cgggtatcaa agaaaaatgc gcctattgtt ataattattg gcacaaacga tattcaaaca
     1501 aatggcgtga gattggaaac aaaagtcatt gagcttggag aaaaacaagg gctaaaattt
     1561 gaacttaatt taaagaagcc gatccgaggg ctacaaaata caatgaagga agaatctata
     1621 ttatttttta ctaatcaaaa ataacattat gaaatacaca aaacctacaa ccgaattgaa
     1681 atttaaaacc gtgattaata aaaatacttt ttattttttt aatccagctt ttgaagaaaa
     1741 atatgaaggt tatctaaact cgttaaagga aatgcttttg atattgaaaa ataatattga
     1801 aacgcaggga ttgaaaaagg aattttttga acagctattg attgaaaaag aaaacggtct
     1861 tagagcgttg ctcgctttga ccgggttttc aaacgaaact ttaaaaagat tgactacaat
     1921 tattagggtt gttaatgatc cagaattgag caagttggtt tataaggaca aatggtttaa
     1981 ggatgaagac ataaataatc tgaaagagtg gtcagatgct caaatcgcaa aatttataaa
     2041 agaaaacgaa ttttttagaa aaggaatttt aaatattttc tttgaaggtt caacagttcc
     2101 gtttttgaca aaaactattc cgctatttga attgaagaaa ctaagcattt caaaactaaa
     2161 atttgaattg ccggaaatga tagacacttt gatcagatac aaggaaaaag gcagttattc
     2221 aggaatgaaa gaaaataatc cagaaatttt aattgaagaa attttgaatc atttagaaat
     2281 accgtttgaa aaaggcgacc tcagcgaact cattgacaat gcccccgata ataaaagaac
     2341 aatggatttt attattccaa acaaacaaaa tccgttaatt attgccgaaa gttcattttt
     2401 ggtaactact tcatccggtc aaggcgataa atcaaaaaca gagatttcta ttgattcgct
     2461 tatcaagtcg cactatccaa aagcaaaatt tattggtttt gtggacggta ttggttggta
     2521 tgtcagaaaa ggtgatctaa aaagaatggt tacggcctat gaagatgttt ttacttttca
     2581 tagagaagaa ttaaaaagat ttgagaaaat tttaattgaa acctttctta gcaaatagtt
     2641 atgatgaata attttttgaa caaaataatt aaaggcgatt gtttggaagt attaaaaaaa
     2701 ataccggaca actcggtgga tatgactttt gccgacccac cttttaattt aaagaaaaaa
     2761 tataatcact acgaagattc aaaagaaaaa caagattatt taaattggtg caatgattgg
     2821 atgagcgaaa tggtgagaat tacgaaacct acgggagcta tatttgttca caatatccct
     2881 cgttggcttt cgcattacgc tgagcattta aacaaaattg cttatttcag acattggatc
     2941 gcttgggatt ccggcggcgc gccaatggga aaaactttat tgccaaatca ttatggtatt
     3001 ttgtattaca caaaatcaaa atctcacaaa gattttaagt tttatgatat tagataccca
     3061 caccccagat gtcgagcgtg cgatgaattt ttaaaagatt atggcgggaa gaaagatcag
     3121 gcgcatggat ttggcccgct tctttctgat gtttggaatg atattcaccg aataaggcac
     3181 aaaaagcgaa gagatgaaca cccttgccag cttcctatcc cgctacttga aaggttaatt
     3241 ttaatgacca ctgatgaagg cgatgttgtt tttgatccgt ttatgggaac aggcacaaca
     3301 gcagtcgcgg ccaagcaatt aggcaggaaa tatatcggca tagaacttga ttctgattat
     3361 gttaaaatcg ccgagaaaaa tataaaagcg acaaaggaaa tgaaaatcaa cggctatttt
     3421 gtttctgttt atcttgggca cattagaaca atgagaaata aggatttcaa agaaatttgg
     3481 aaaaacaaag gcgatttaaa attgcttgat aaaaagaaat ataaaatagt taagacaacc
     3541 gaataaacaa aatatggcta ataatctatt atcaaaagga tcggtttgga gaaaatggga
     3601 tttgcaagtc cagacaagat tggatgcaaa ttatacttgt ctcgggaata atagtttgag
     3661 tgcagctgat ctacaaaaat tgataacagc aaccggactt actgaaacag aaattactgc
     3721 tcaggagaaa gagattgatg cccaaaaata cgctaagcta tttataaaat atgttgaatt
     3781 ttttacggac ataagtgttt ttggtatcac cgaccataat acaggtaggg aaattgacgc
     3841 acttataaat gaggcaaaaa attcatctcg ggaaattgcc attttgcccg gcgttgaagt
     3901 ttcatctgca cagggattac atattttgtg catatttgat ccagaaaaaa agtggcgcga
     3961 caattgggct gaatcaatag accatttcct aacagagtta ggattaacaa atggcgtttt
     4021 taatacccaa aatcagccgt taaactcaac gaaaacagct caagaaatat taacgattac
     4081 caacaaaaaa ggtggcctgt gtatttttgc ccatattggg acggataatg gtctttttaa
     4141 attttccaac acagcaagcg gtggcaatgc tcatatttca gtttataaag acaaactttg
     4201 ccaaatagtt caattgcccc gtagcgcaaa tttatccaat ggagtgcaaa atattatcga
     4261 gggaaaggat ccccaatatg gcaacaaaag cgtaactcaa attaaatgct ccgacgcaag
     4321 aaaattaaca gaaattggag cttgctttac ttggattaag gccgaccaaa catttgatgg
     4381 tcttaagcaa ataatttttg agccggaaga tagggttaaa attcaggaaa actcaccata
     4441 cgaagacaga aaaaagattt atttcgaaaa tttgacaatc gacgggcgga ttaattttat
     4501 cttgcccaat attacaattc cgcttaattc agaattgata acggttattg gcgggcgagg
     4561 gagcggtaaa agtgcgcttt tggaagcgtt cgcgttttta aatgaggagc atttgaaagt
     4621 cgaccaaaat ggtaaaaaga aaataattga attttataga gacaatgaag tagcaactga
     4681 tccagcgcca agttttatta ccaaaacaac tttagtcgac aaggacttga ataagcaaga
     4741 atttcaaaaa caacttgccg aacatgaaaa tttagaactt ccatttttat atttagggca
     4801 agagcaatta agcgggcttg cgaccaacga tatagaattg actaaaacaa tttgcgagct
     4861 tattggcatt gatattgccg aattaagcca acaagcgcat atttttaaag caagagaaat
     4921 acttgcacag attaaaaaca ccaaccaatc cctcggtgat gtttttaata tctataagca
     4981 aattggatat aatgaaacga ctgactttga aaaatggatt gcgtcatatt taaaaaaact
     5041 tggcgcacaa caaaaacgcc tcacttcaaa agaaacccgc accacccttg aagaaataaa
     5101 taaaaacacg caacgaggac tcaaactaaa agagcttctt gaacaaattg atgaaacagc
     5161 caaagaatta aacactcttg tcgtaaacac aagtatcaat tctttaaata aaagattggc
     5221 tggtttatat cctgatgaaa agccgacaat aactccaatc gattcaaaaa aacagcttgt
     5281 cgaaattagc cagattcaag ggcagattaa aaccgaaatg gatacattgc gaaaagatat
     5341 tgttgggaaa aaagccgaac tttcgaaact gggcataaaa gaagatgtaa atacactttt
     5401 gcagtccagc caaacgctac aacaacaaat aaataatgcg aataaggatt tagaaaatta
     5461 caaaaacgca attataaggc tttccgaatt tatcaaacag cgcaatgata ttcttgtaaa
     5521 tgtggagggc ggactaaacg aattaaaaga gcagatagat gtaaaatttg ccgaatttaa
     5581 gagttctcgc aacgattctg aagcacaaga gaaagaattg tttgaaaaaa taattcaagg
     5641 cattgaaatt gagggtgata ttgtttttga tgaggagcaa ttttgcaatg atgttctcgg
     5701 tagctttgtt gataatagaa aaattaaaaa cgagaatgat ctcagggaag aaattgccgg
     5761 taagaatact gacggcacag caaaacacat cacgctaata tctattgcca attgggtcaa
     5821 acaagattta tcaaccaaac tttatttcaa taggagcgga gtagacggaa tgcttgaata
     5881 tatttttacc aactggcctc aatttatgcg agttcgggca attgtaaaat tgaatggaaa
     5941 atcgacggaa gtattatcga ttggccaaag aggaacatta ttattgaaag tttttcttgc
     6001 tacagcaacg gcaaaacagg tgtttatcat tgaccaacca gaagataacc tagataataa
     6061 ttttatcatg cacgaattgg ttccgctcat ccgaaaagcc aaagtttctc gtcaaattat
     6121 tatgtctaca cacaatgcca atttagtcgt aaatgcagac gcagaacaaa tcattgttgc
     6181 gcgtcttgac aatgaaggtg attatataag cggtagtatc gaaaaccaaa ctatcaatca
     6241 aagcattaaa gaaattcttg aaggtggcga ggaagcattt agaaatcgtg aaaataaata
     6301 tggcatggct ataataaatt gaggccccgc cgtccgctcg caaaaaaatc tggccgtcca
     6361 atcaggcggg cgaaaag
//
LOCUS       DAOQWE010000037         6298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_12738_length_6298_cov_8.051097, whole genome shotgun sequence.
ACCESSION   DAOQWE010000037 DAOQWE010000000
VERSION     DAOQWE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_6298_cov_8.051097"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKK37_02075"
     CDS             complement(<1..209)
                     /locus_tag="PKK37_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81197.1"
                     /translation="MLLVSPKQKITQFLGDLVVCDPARRCARQAKTIILPRKSQNNNI
                     DNNNNITMREKLFFEFRGDDNGVGD"
     gene            224..1621
                     /gene="glnA"
                     /locus_tag="PKK37_02080"
     CDS             224..1621
                     /gene="glnA"
                     /locus_tag="PKK37_02080"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNR81198.1"
                     /translation="MEYREIIEKCKKENVKLVDLRFCDLPGAWQHFAVPIGQFEEKIF
                     SEGIGFDGSSIRGFKSIEESDMILIPDAKTFIVDRFGKEPVGGLICDVYNPESKTRFE
                     KDPRAIAQTAEKFLKESGIADTAYFGPEAEFFIFDRVEFASGQNQSFFRVQSSEAGWE
                     NNGEDAGSGHKIRNKEGYFPMAPADQTMDIRREMVAEMQKLGIEVEKEHHEVATAGQA
                     EIDIKYDTPAGQADKIMMFKYAVKNVAHRFGKTATFMPKPLFGDNGSGMHTHISLWKN
                     GENLFFDKKGYAQLSQTALWFIGGLLAHGRSLMAFCAPTTNSYKRLVPGFEAPTNLAY
                     SARNRSAAIRVPMYDNSPAAKRIEFRPPDASGNPYLAFAAMMMAGIDGIKNKTDPGQP
                     ANGDIYKNNGGILQVPASLEESLAALETDHEYLLAGGVFGASLIDCWISFKKNECDFC
                     RLKPTPAEFELYYGI"
     gene            complement(1663..2256)
                     /locus_tag="PKK37_02085"
     CDS             complement(1663..2256)
                     /locus_tag="PKK37_02085"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNR81199.1"
                     /translation="MLDLHRNLFKGLESKVADIFSRLPFTPNQYTSLSIMAALAMLCL
                     IAASHYIATLALFIVAAGLDFIDGAVARKRGLATKRGAYWDTIADRYVEAIFLFGLLF
                     CGLPDFFLPAAAWIFLALFGSTMTTYAKAAAKEKGLCNAELKGGLMSRAERLIAYFAI
                     IILLLIDLYLAVAILAALAILSNFTALQRIYCALKFK"
     gene            complement(2326..3447)
                     /locus_tag="PKK37_02090"
     CDS             complement(2326..3447)
                     /locus_tag="PKK37_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNR81200.1"
                     /translation="MANNNAEGKKIRLAIAGIGNCCSSLVQGITYYKYIGKDDEPVPG
                     LMHNVIGDYAPRDIQIAAAFDIDRRKVGKDVSAAIFEKPNCTKIFCKDIPETGVKVQM
                     GPVLDGVADHMQDYPEEQSFVVADEKPVNVAEELKKAKADVLVSYMPVGSQKAAEYYA
                     QAALDAGCGFVNCMPIFIVSDEKWAAKFKEKNIPCVGDDIKSQIGATIVHRTLTKLFA
                     NRGVIVDRSYQLNFGGNTDFLNMLSRDRLKSKKISKTESVESQLNKRLSYDNLHIGPS
                     DWVPFLKDNKICFIRMEGRKFGDIPVEIELRLSVEDSPNSAGVVIDALRMVKLAKDRG
                     IGGPLTSPSSYFMKHPPVQFTDSQAYEMVEEFIAGKRDH"
     gene            3681..3986
                     /locus_tag="PKK37_02095"
     CDS             3681..3986
                     /locus_tag="PKK37_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81201.1"
                     /translation="MFPVQRLEKSNTKENLWVYILALLEKTPRYGWEIPALIEKKFGF
                     RPGKITPYRVFYRLQADGFVRSKTADRRRIYEITEKGKKELAAAKLFYKNILSKLDG"
     gene            3979..4644
                     /locus_tag="PKK37_02100"
     CDS             3979..4644
                     /locus_tag="PKK37_02100"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81202.1"
                     /translation="MDNKKIAENLNEIADLLDIKGEKPFRIRAYRKAAEILQILENNL
                     AEFYKKEKKLPKIKGIGESIGGKIEELIKTGKIKYLKELQEDTAIRQVITCFFETKGV
                     NLDELKRSARKRDIVYSRYTKPAKQLIELAGSPQKAKDAIKKVADWANSRKLDYAIET
                     VFKKWLELDRLKPKEVVKKPFYRGDPMIWSENRKKWFVITAAGEWLEYADKEELIEWR
                     IID"
     gene            4835..5395
                     /locus_tag="PKK37_02105"
     CDS             4835..5395
                     /locus_tag="PKK37_02105"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNR81203.1"
                     /translation="MLNYYDLRRGVQFILDGQPHEVLEFQQIKKARAAGLLQAKIKNL
                     ITGKVLERTFHQGDNFEETEIEKISAKFIYANKDKFVFCRAGDPSKRFELSEDQIGGS
                     AKFLAANMEVEANIYENQIIGVNLPIKVQLKVKEAAPGVKGNRAQSGTKAVVLETGAT
                     IQAPLFVQAGDVLEINTETGEYSKRV"
     gene            complement(5522..5842)
                     /locus_tag="PKK37_02110"
     CDS             complement(5522..5842)
                     /locus_tag="PKK37_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81204.1"
                     /translation="MIETIFYFFGALFFLTWFLLLLVVLFFSIKAFRRLVAIENEVKG
                     AVGEAKATISDVRNKVVSFSISVAGIGALLERLIEIKNKAKEKRSGAPKKEKKARKFA
                     EEDF"
     gene            complement(5915..>6298)
                     /locus_tag="PKK37_02115"
     CDS             complement(5915..>6298)
                     /locus_tag="PKK37_02115"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNR81205.1"
                     /translation="LYYYNPENDKDESGNIMCSPAGLVAVGREIAVTPTPIQDAVNLL
                     IKGDLTDEEIAAGITSEFPLAGVELKGANLKEGILTLEFADPQNKTGGGACRTGILRA
                     QIEATAEQFDGVNQVRFIDEELFQP"
BASE COUNT         1793 a         1517 c         1386 g         1602 t
ORIGIN      
        1 aaatcgccaa cgccattatc atctcctcgg aattcaaaaa atagcttttc tcgcattgta
       61 atattattat tattatcaat attattattt tgacttttgc gcggcaaaat gatagtcttt
      121 gcttgtcggg cacaacggcg tgccggatcg cagacaacga ggtcaccaag aaattgggtg
      181 attttttgtt ttggcgatac caataacaat ctgatttaaa tttatggaat atagagaaat
      241 aattgaaaaa tgcaaaaaag aaaatgtgaa gttggttgat ttgagatttt gcgacttgcc
      301 cggcgcgtgg caacattttg ccgtgccgat tggtcagttt gaagaaaaaa tcttttccga
      361 gggcatcggc tttgacggct catcaattcg cggattcaaa agcattgaag aaagcgatat
      421 gatcttgatt cccgatgcaa agacctttat cgttgatcgc ttcggtaagg aaccggtcgg
      481 cgggttaatt tgcgacgttt ataatccgga atcaaaaacc cgttttgaaa aagacccgcg
      541 ggcaatcgcc caaaccgcag aaaaattttt aaaagaaagc ggaattgccg atacggccta
      601 tttcggcccc gaagcggaat tttttatctt tgaccgggtt gagtttgcca gcgggcaaaa
      661 ccaatctttc ttccgcgtcc aatcaagcga agccggctgg gaaaacaacg gcgaagacgc
      721 cggttcgggg cataaaatcc gcaacaagga aggctatttc cccatggcgc ccgccgatca
      781 aacaatggat atccgccgcg aaatggtcgc tgaaatgcaa aaactcggca ttgaagtgga
      841 aaaagagcat cacgaagtcg ccaccgccgg gcaggccgaa attgacatta agtatgacac
      901 gccggccggg caagccgaca aaataatgat gttcaaatac gcggtcaaaa atgtcgcgca
      961 tcgtttcggt aaaaccgcca cttttatgcc caagccgctg ttcggcgaca acggttcggg
     1021 aatgcacacc catatttctc tttggaaaaa cggcgaaaat ctgttttttg acaaaaaggg
     1081 ttacgcacaa ttgagccaaa ccgcactttg gttcatcggc ggactgctgg cacacggaag
     1141 atcgctgatg gcattctgcg cgccgacaac caattcttac aagcggctgg tgcccggatt
     1201 tgaagcgccg accaatcttg cttactcggc gcgcaatcgt tccgcggcaa tccgcgtacc
     1261 gatgtatgac aattctccgg cggccaaaag aattgaattt cgcccgcccg acgcgtccgg
     1321 caatccctat ctggcattcg cggcaatgat gatggccggc attgacggca ttaaaaacaa
     1381 aaccgatccg ggacaacccg ccaacggcga tatctataaa aacaacggcg gcatccttca
     1441 ggtgccggcc tcgctggaag aatcgctggc ggcgctggaa acggatcacg agtatttgct
     1501 cgccggcggc gtgttcggcg cgtccctgat tgactgctgg ataagtttca aaaaaaatga
     1561 atgcgatttt tgccgcctca aaccgactcc cgcggaattt gaactttact acggaatttg
     1621 aaaaacgggc cgcaaggccc gttttttcat cttcattgtc gcttatttaa actttagcgc
     1681 gcaataaatt ctttgcaatg cagtgaaatt ggacaatatt gccaatgccg ccaaaattgc
     1741 tactgccaaa tataagtcaa ttaaaagcag gataatgatc gcgaaatatg ctatcagccg
     1801 ctcggcgcgc gacatcaatc cccctttcaa ttcggcattg cacaatccct tttcctttgc
     1861 cgcggctttg gcataggtgg tcatcgttga accgaagagc gccagaaata tccacgccgc
     1921 cgccggcaga aaaaaatcgg gcaacccgca aaacagcaat ccaaaaagga agatcgcttc
     1981 cacatatcgg tccgcaatcg tgtcccagta ggctccccgc ttcgtggcca ggcccctttt
     2041 gcgcgccacc gcgccgtcaa tgaagtccaa gccggccgcc acgatgaaca acgccagagt
     2101 ggcaatataa tggctggcgg caatcaaaca cagcatcgcc aatgcggcca tgattgaaag
     2161 cgaagtatat tggttgggag tgaacggcaa tcgcgaaaaa atatcagcaa ctttgctctc
     2221 caatcccttg aataaatttc tatgaagatc cagcatcagt gatttaagat ttagttggcg
     2281 agatttcacc cagacaagct tgtctgggtg aaatctcgcg ccattttagt ggtcgcgttt
     2341 gccggcgata aattcttcca ccatttcata ggcttgggaa tccgtgaact gcaccggcgg
     2401 atgtttcata aaataagacg acgggctggt gagcggaccg ccgattcccc ggtccttggc
     2461 caatttgacc atccgtagag cgtcaattac cacgccggcg gaattgggcg aatcctccac
     2521 cgacaggcgc aactcaattt ccaccggaat gtcgccgaat tttcggcctt ccattctgat
     2581 gaaacaaatt ttattgtcct ttaaaaacgg cacccaatcc gacggcccga tatgcagatt
     2641 gtcataactc aatcttttgt tcaattgcga ttccacgctt tcggttttgg agattttttt
     2701 tgatttcagg cgatcgcgcg acaacatatt caaaaagtcg gtgttgccgc cgaaattaag
     2761 ctgataggag cgatcaacaa tcacgccgcg gttggcaaac agtttggtca aagtgcggtg
     2821 cacgatcgtg gcgccgattt gcgatttgat gtcgtcgccg acgcagggaa tgtttttttc
     2881 tttgaatttg gccgcccact tttcatcgga aacgataaat atcggcatac aattcacgaa
     2941 cccgcaaccg gcgtccaacg cggcttgcgc gtaatattcc gcggcctttt gcgatcccac
     3001 cggcatataa ctcaccaaaa cgtcagcctt ggcttttttc agttcttcgg cgacattgac
     3061 cggtttttca tccgcaacaa caaacgattg ttcttcggga taatcctgca tgtggtcagc
     3121 caccccgtcc agcaccggcc ccatttggac ctttaccccg gtttcgggga tgtctttgca
     3181 aaaaattttg gtgcagttgg gtttttcaaa aatagccgcg gaaacatcct tacccacttt
     3241 gcgccggtca atgtcaaacg ccgccgcgat ctgaatgtcg cgcggcgcgt aatcgccgat
     3301 cacgttgtgc atcagccccg gcaccggctc gtcatccttg ccgatgtatt tatagtaagt
     3361 gatgccctgc accaggctgg aacagcagtt gccgatgccg gcaatcgcca gccggatttt
     3421 cttcccctcc gcattgttgt ttgccatatt tttgttagaa taaattatgg tatgttttac
     3481 attatgtaat tattttacca ttgtaaaata ctatgtcaaa ttccgtaaaa aaaccgctca
     3541 ctgcatctaa aattataaaa tggtctatcc ctcgttaatg cgagggttag atccttacca
     3601 gcaagcgatt atccgctgga gatcaaccaa ctttcggctt taatttttta ctttttcatt
     3661 tttaattttt aattattttt atgttccccg tccaacgtct ggaaaaatca aacaccaagg
     3721 aaaacctctg ggtctatatc ctggcgcttt tagaaaaaac tccgcgctac ggctgggaga
     3781 ttccggcgct gattgaaaag aaattcggct tccggccggg aaaaatcact ccctaccggg
     3841 tgttttaccg cctgcaagcc gacggattcg taagaagcaa aacggcagat cgccggcgca
     3901 tctatgaaat caccgaaaag ggcaaaaagg aactggccgc cgccaaatta ttctataaaa
     3961 acatcctatc aaaattagat ggataacaag aaaattgccg aaaatttaaa cgagatcgcc
     4021 gatttgctgg acattaaagg cgaaaagccg ttccgcatcc gcgcctatcg caaggccgcg
     4081 gagattttgc aaattctgga aaacaatttg gccgaatttt ataaaaaaga aaaaaaatta
     4141 cccaagataa agggtatcgg cgaaagcatc ggcggtaaaa ttgaggaatt gataaaaacc
     4201 ggcaaaataa aatatttaaa ggaattgcaa gaagataccg ccatccgcca agtgattacc
     4261 tgcttttttg aaaccaaggg cgtgaatctt gacgaactga aacgcagcgc ccgcaaacgc
     4321 gacattgttt attcacgcta caccaagccc gccaaacaat tgattgaatt ggccggatcg
     4381 ccgcaaaaag ccaaagacgc gataaaaaaa gttgccgatt gggcgaattc ccgcaaactg
     4441 gactacgcca ttgaaacggt gtttaaaaaa tggctggagc tggaccggct taaacccaag
     4501 gaagtggtca aaaaaccgtt ctatcgcggc gatccgatga tttggtcgga aaaccgcaaa
     4561 aagtggtttg tgatcaccgc cgccggtgaa tggctggaat atgccgacaa agaagaactg
     4621 attgaatggc gcatcattga ctaaagccaa gaatcaaatt atgatttatc tgcgatttca
     4681 gtgaatatca aattgattcg tttttttgtt tttaaattaa tttttaattt gaaatttgaa
     4741 atttttaaac aaggtttaat tttgaaattt ttaaacattg aaatattaaa aattgtttga
     4801 aaattaaaaa ttataaatta aaaattttat aacgatgctt aactactatg atttgcggcg
     4861 cggcgtgcag tttattctgg acggccaacc ccacgaagtg ctggaattcc agcaaatcaa
     4921 aaaggcccgc gccgcgggac tgcttcaggc aaagatcaaa aatctcatca ccggcaaggt
     4981 gctggagcgg actttccacc aaggcgacaa ttttgaggaa accgaaattg aaaaaatttc
     5041 ggcaaaattc atttatgcta acaaggacaa attcgtcttc tgccgcgccg gcgatccctc
     5101 aaaaagattt gaactttctg aagaccaaat cggcggaagc gcaaaatttt tggcggcgaa
     5161 tatggaagtg gaggccaata tctatgaaaa tcaaatcatc ggcgtaaatt tgcccatcaa
     5221 ggtgcaatta aaagttaaag aagccgcgcc gggagtgaag ggcaaccggg cacaatcggg
     5281 caccaaggcg gtggttttgg aaaccggcgc gactattcaa gcgccactgt tcgtccaagc
     5341 cggcgacgtt ttggaaatca acaccgaaac cggcgaatac tcaaaaagag tataataatc
     5401 aattatcatt gataattgat aattgattat ttataattta taatttatga ttgataattg
     5461 atcaattatc aatcgccacc aaaccaaaaa acgcctgacg gcgttttttt gatgaaaatt
     5521 tttaaaaatc ttcttcggcg aattttcttg ctttcttttc ttttttcggc gcccccgatc
     5581 ttttttcctt tgctttattt tttatttcaa tcaatctctc cagcaacgcg ccgatgcccg
     5641 ccacactgat ggaaaaagaa accactttgt tgcgcacatc gctgattgtc gctttcgctt
     5701 ctccaacggc acccttgact tcattttcaa tggcaaccaa tcgccggaaa gcttttattg
     5761 agaaaaacag caccaccaac aacagcaaaa accaagtcag aaaaaacaat gcgccgaaaa
     5821 aataaaaaat ggtttcaatc atgaatggaa attatttttg ttttgcgttt ccattttcgc
     5881 gcgaaaatcc tgccgcgtca agccgcaaaa aaatttacgg ctgaaataat tcttcgtcaa
     5941 tgaatcgcac ttgattgacg ccgtcaaact gctcggcggt cgcttcaatc tgcgcccgca
     6001 gaattccggt gcggcacgcg ccgccgccgg ttttgttctg cgggtcggca aattccaaag
     6061 tcaaaatccc ctcttttaaa ttcgcgccct tcaattcaac tcctgccagt ggaaattcgc
     6121 tggtgatacc cgcggcaatc tcttcatcgg ttaaatcccc ttttatcaat aaattcaccg
     6181 cgtcttgaat cggcgttggc gtaaccgcaa tttcgcgtcc aacagcaacc agtcccgcag
     6241 gagagcacat tatattcccc gattcatcct tatcattttc gggattgtaa taatagag
//
LOCUS       DAOQWE010000038         6248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_12855_length_6248_cov_7.983691, whole genome shotgun sequence.
ACCESSION   DAOQWE010000038 DAOQWE010000000
VERSION     DAOQWE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12855_length_6248_cov_7.983691"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKK37_02120"
     CDS             <1..209
                     /locus_tag="PKK37_02120"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491938.1"
                     /note="catalyzes a two-step reaction, first charging an
                     alanine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNR81206.1"
                     /translation="LSEAQQSGAMGVFGSKYGDRVTVYTIGDFSKEICGGPHVENTGK
                     LGNFKIIKEESSSAGVRRIKAVLG"
     gene            304..816
                     /locus_tag="PKK37_02125"
     CDS             304..816
                     /locus_tag="PKK37_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81207.1"
                     /translation="MDKKIIYYAIGGLLLILAVWGIIAINSQKKETETPPSITPPVMQ
                     KSETEVNRDVAQKLELAEGQLPLRVTRDDNRQAASLTPGKNITLMLGEEYGWTISSSN
                     DKVLAPRNVDIDDARVQGVYQVVGEGNAVLTASGNCKSGAVCETPSADFTFTVEGIIS
                     ENFSPEELTR"
     gene            882..1448
                     /locus_tag="PKK37_02130"
     CDS             882..1448
                     /locus_tag="PKK37_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HNR81208.1"
                     /translation="MKIFAGTANKIKIEAVFEIAADYEFLSAAEVEGVDVPSGVADQP
                     KNLAETVGGAKNRARAAFLAGDAHPGDLGIGLEDGLMAVPESRTGLMNIGICAVYDGK
                     DFCLGASAAFEYPPAAIELVKKGMDINQAFHSLGLTDNPLVGSAQGAIGILTHCRWDR
                     KETAKQALTAALIQMENKDLYGGRGVSA"
     assembly_gap    1576..1675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1676..2166)
                     /gene="infC"
                     /locus_tag="PKK37_02135"
     CDS             complement(<1676..2166)
                     /gene="infC"
                     /locus_tag="PKK37_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNR81209.1"
                     /translation="MFKKPPINNQIRAAQVRLIDENDQQIGVVALKDALEMAWSKKLD
                     LIQVTDKVNPPVAKIAEFGKYLYNLQKKEKNVHTSEVKGIRLTYNISPHDMETRAATA
                     EKFLKKGDRVKIELRLRGRQKAFGNLSREKVEQFLSILEKHIPYKVDKELKNEMGGFN
                     MIIS"
     gene            complement(2168..2525)
                     /gene="ssrA"
                     /locus_tag="PKK37_02140"
     tmRNA           complement(2168..2525)
                     /gene="ssrA"
                     /locus_tag="PKK37_02140"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2531..2992)
                     /gene="smpB"
                     /locus_tag="PKK37_02145"
     CDS             complement(2531..2992)
                     /gene="smpB"
                     /locus_tag="PKK37_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNR81210.1"
                     /translation="MKELAKNKKAGFDYEILEKFEAGLVLNGQEAKSIKTRGLSLAGS
                     YVIVRPDGVFWIGAKIPPYQPANAGADYNERRDRQVLLRRAEIRRLVGIAAQKGLTFV
                     PIRLYTKQRYQDAGKIKLEFAVCRGKKKFDKRQALKTRDTEREIARSFGKI"
     gene            3075..4703
                     /locus_tag="PKK37_02150"
     CDS             3075..4703
                     /locus_tag="PKK37_02150"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNR81211.1"
                     /translation="MMLREKIGLLVNDAVRKLATAGFLGSADIDKIIGPGALSACKDA
                     NHGDYASNIAMRLAGAAKKPPLEIAQKIAEEINQNKKAAKIFKKIEVAAPGFLNFTLS
                     DKYVLGETEKILKLDKNYGRLKIGKNKKTQVEFISANPSGQLHVGNGRSAFYGDALGN
                     VLAMSGYKTEKEYYVNDARVSKQIQTLGATALGRGEAYLSPYLKEKIASLQKKLSKIR
                     SETDAGYFLAQAVLKDLQKFIAKDLKIKFHEWIFEECLYKRGLIIKTYDFLKKKGLVF
                     EKDGAYWLDMSKYGQKDEVLLRSNGSPTYFLSDIAYHHNKIKRGYKKIIDIWGADHQG
                     HVPRMRAVMDILGYKGEFEALICQIVTIKGGKISKREGNIVSLNWLIDEVGVDAARFF
                     YLQNSLSAQMEFDVELAKKKSADSPVFYVQYAHARIASILRKAGLKGSGPFNSKRVRP
                     FQELRHPAEIELAKELLRLPEIVADTAKDYQIQRVCLYAASIAAKFHKFYHDCQVIGE
                     DKKTTRARLGLAMASKIVLKNTLGLLGISAPEKM"
     gene            4763..5296
                     /locus_tag="PKK37_02155"
     CDS             4763..5296
                     /locus_tag="PKK37_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNR81212.1"
                     /translation="MFEFSHSKKQKGFTLIELLVVISIIGVLSGLVMVTMQDMRAAAR
                     DARRKQDLAQIAKAVELYNIDYGRYQILNSGWGSDTGTGCGCGWFSAEGGAYGRSIAR
                     ALVEDGGKLSILVKDPTGGTSCSTTAGYAYMLYLRGSGFYVYAKLEKPNAADLATCDA
                     NAQCPTNYGMNYAVGHR"
     gene            complement(5312..5998)
                     /locus_tag="PKK37_02160"
     CDS             complement(5312..5998)
                     /locus_tag="PKK37_02160"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HNR81213.1"
                     /translation="MAKLNGKKRYLQIALNGDLADAQKIIARLPASDRIIIEAGTPLI
                     KEHGITAVSRLNYWWQKRLDSAKINAPAYVVADLKTIDRGATEAQMAAGAGASAAVAM
                     GQAPIETLNSFIAECENLGIDAMIDMMNVEYSLNVLRRLKKQPAVVVLHRGVDEEIFN
                     REKQIPYHEIQRVKGNYDIMLAIAGGDTPREVQRAFFNSADIAVVWKNFYTATGDTVA
                     LAQDFLRQIK"
BASE COUNT         1571 a         1367 c         1623 g         1587 t
ORIGIN      
        1 cattgtccga ggcacagcaa tcgggtgcga tgggggtctt tggatcaaaa tacggtgatc
       61 gggtaacggt ttatacgatc ggggatttct ccaaagagat ttgcggcggg ccgcatgttg
      121 agaataccgg aaagttggga aattttaaaa tcatcaaaga agaatcgtcg tcggcgggag
      181 tgcgccggat aaaggcggtt cttgggtagc cgttttccac aaggcgatat ttgcgttgtt
      241 ttttgaaatt gctagaatga agttgtagaa tatataaaac tccggaaagc ggagaaaaca
      301 aatatggata aaaaaataat ttattatgcg atcggaggct tgttgttgat tttggcggtg
      361 tgggggatta ttgccataaa ttcgcaaaaa aaagagacag aaaccccgcc ttcaataact
      421 ccgccggtaa tgcaaaaatc ggaaaccgaa gtcaatcgcg atgtcgcgca aaagcttgaa
      481 ttggccgaag gccaactgcc tctgcgggtg acgcgggacg acaatcgcca ggcggcatcg
      541 cttactcccg gtaaaaatat tactttgatg ctgggggagg agtatggctg gacgatatct
      601 tcaagcaatg ataaagtgct ggcgccgcgc aatgtggata ttgacgacgc gcgcgtgcag
      661 ggagtttatc aggtggtggg cgaggggaac gcggttttga ccgccagcgg caattgcaag
      721 agcggcgctg tttgcgaaac cccttccgcg gattttacat ttaccgttga ggggattatc
      781 agtgaaaatt tttctccgga agaattgacc agataaaaaa ctaaaatttc aatcaaaacg
      841 gctttttgaa agccgttttt gttgaaatcg gaacaagggt tatgaaaatt tttgcgggaa
      901 cggcgaataa aatcaaaatt gaagcggttt ttgaaatcgc cgccgattat gaatttttat
      961 ccgccgccga ggttgaaggc gttgatgttc ccagcggtgt ggctgaccag ccgaaaaatc
     1021 tggccgagac cgttggcggc gcgaaaaacc gcgcccgcgc cgcgtttttg gccggcgatg
     1081 cgcatccggg agatttgggc atcggtcttg aggacgggtt gatggcggtg cccgaatccc
     1141 gcaccggatt gatgaacatc ggcatttgcg ccgtttatga cggcaaagat ttttgcctgg
     1201 gcgcttcggc ggcgtttgag tatcctcccg cggcgattga attggttaaa aaagggatgg
     1261 acatcaacca agcttttcat tctttgggtc tgacggataa tccgttggtg ggatcggctc
     1321 aaggcgcaat cggaattctg acgcattgcc ggtgggaccg gaaggaaacc gccaagcaag
     1381 cgcttaccgc ggcattgatt caaatggaaa acaaggactt gtatggcgga cggggagtat
     1441 ccgcttgaaa cgaaaaacgc ctcccggatc gggggcgttt tttgtttgcg ggcattaatt
     1501 atcttttggt ttttcccagc accgcctcaa acaaatgagg atatttggcg gaaaactccg
     1561 aaagaatttt gcggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatat
     1681 tatcatattg aatccgccca tctcattttt taattccttg tcaactttgt aggggatatg
     1741 cttttccaaa attgacagaa actgctccac cttttcccgg gaaagattgc cgaacgcttt
     1801 ttgccgcccg cgcaggcgca attcaatttt cacccggtcg ccctttttca gaaatttttc
     1861 cgcggtggcg gcgcgcgttt ccatatcgtg cggagaaata ttataggtca ggcggattcc
     1921 cttcacctcg ctggtatgga cgttcttttc tttcttttga agattgtaaa gatattttcc
     1981 gaattcggcg attttagcca ccgggggatt caccttgtcc gtcacttgga tcagatcaag
     2041 ctttttgctc cacgccatct ccaaagcgtc cttcaacgcc accacgccga tctgctggtc
     2101 attctcgtca atcaggcgca cctgcgccgc ccttatttgg ttgttgatgg gaggtttttt
     2161 gaacattatg tggagacgac gggacttgaa cccgtatctg gcaaatccgg caaatggcgt
     2221 ctacaagcgt agtccggttt ttttaagaat caaagattga aatcggacaa aattctttga
     2281 ctctccgccc gaagttggtt tcaaaaaacg gttcggggca agaagcgttt ttctatctcc
     2341 caaagattat gcccggcaag tttgagggag aatccgcccg ccggacaccg aatcggatac
     2401 taagcgtaaa cgagttcggg agtgctgtat gatttggcac ttttggagtt ttgccgattt
     2461 taacgagatt cggcgactcg gcttgcaaca atttggctga tttgccatcg aatccttgca
     2521 tccccaaatc ttaaattttt ccgaaactgc gggcgatttc ccgctcggtg tcgcgcgttt
     2581 tcaacgcctg gcgcttgtca aacttctttt tgccgcggca aacggcgaac tccaatttga
     2641 tcttgccggc atcttgataa cgctgtttag tatataacct aatcgggaca aaagtcaagc
     2701 ctttttgggc ggcgattccg acgaggcggc ggatttcggc ccggcgcaac aaaacctgcc
     2761 gatcgcgccg ttcgttatag tcggcgccgg cgttggcggg ctgatagggg gggattttgg
     2821 cgccaatcca aaaaacgccg tccggacgaa cgatcacata cgatccggcc aaggacagtc
     2881 cccgggtctt gattgatttg gcttcctggc cgtttaaaac caaacccgcc tcaaactttt
     2941 ccaagatttc ataatcaaac cccgcctttt tgtttttcgc caattccttc atttcccttt
     3001 tattttatca gaattcaggt taaactgaaa ccggttaaaa gtaaaatcat catctaatta
     3061 ttattcatat ccttatgatg ttgcgggaaa aaataggttt gttggtcaac gatgcggtaa
     3121 ggaaactggc gacagccgga tttttgggtt cggctgatat tgataaaatc atcggaccgg
     3181 gagcgttgag cgcgtgcaag gatgcgaacc atggcgacta tgcgtcaaat attgcgatga
     3241 ggctggccgg cgcggccaaa aagccgccgt tggaaatcgc gcaaaaaatc gccgaagaaa
     3301 ttaaccaaaa caaaaaagcg gccaaaattt ttaaaaagat tgaagttgcc gcgcccggat
     3361 ttctcaactt tacgttgagt gataaatacg tgttgggcga aacggaaaaa attttgaaat
     3421 tggacaaaaa ttacggccgg ctgaaaatcg gcaaaaacaa aaaaacccag gtggaattca
     3481 tttcggccaa ccccagcggc cagttgcatg tgggtaacgg tcggagcgcg ttctacggcg
     3541 acgcgctggg caatgttttg gccatgtccg gatacaaaac cgaaaaagaa tattatgtca
     3601 acgacgcgcg ggtgtcaaaa caaatccaaa ctttgggcgc caccgcattg gggcggggag
     3661 aggcctattt gtcgccctat ttgaaagaaa agatcgcgtc tttgcaaaaa aaactttcaa
     3721 aaatccgttc cgaaaccgat gccggctatt ttttggcgca agcggtgctc aaagatttgc
     3781 aaaaattcat cgccaaagat ttgaaaatca aatttcacga gtggattttt gaagagtgcc
     3841 tctacaagcg ggggctgatc atcaaaacct atgatttctt gaaaaagaaa gggctggtgt
     3901 ttgaaaaaga cggcgcctat tggctggata tgtccaaata cggccagaaa gacgaagtgc
     3961 tcctgcggtc aaacggtagt ccgacatatt ttttgtcgga tatcgcttac catcacaata
     4021 aaatcaagcg cgggtataaa aaaatcattg atatctgggg cgccgatcat caggggcacg
     4081 tgccgcgaat gagggcagtg atggatattt tgggatacaa aggggaattt gaggcactga
     4141 tctgccagat cgttaccatt aagggcggca agatttccaa aagagagggt aatatcgttt
     4201 ctttgaattg gctgattgac gaagtcggcg ttgatgcggc gcggttcttc tatctgcaga
     4261 attcattgtc ggcgcaaatg gaatttgatg ttgaattggc caagaaaaaa tccgccgatt
     4321 cgccggtgtt ttatgttcaa tatgcccacg cgcgcattgc cagcattttg cgcaaagccg
     4381 gcttgaaagg gtccggccct ttcaattcca aaagggtccg gccctttcaa gaattacggc
     4441 atcccgccga gattgaattg gcaaaggaac ttttgcgctt gcctgaaatt gttgccgata
     4501 ccgccaaaga ttatcaaatc cagcgcgtct gcctctacgc ggcgtcaatt gccgccaaat
     4561 ttcacaagtt ttaccacgat tgccaagtga tcggcgagga taaaaaaacg acccgcgcgc
     4621 ggctgggatt ggcgatggcc tcaaaaatcg ttctgaaaaa cacgctcggt ctgctgggaa
     4681 tttccgcgcc ggaaaaaatg tagcgcccga ggtatgtttt ttaaactttt cttaaaagag
     4741 acgaggcgca ttaaaacaag agatgtttga attttcgcac tcaaaaaaac aaaaagggtt
     4801 tactttgatt gaattgttgg tggtgatttc aatcatcggg gttttatcag gccttgtgat
     4861 ggtaactatg caagatatgc gcgccgccgc tcgcgacgcg cggcgcaagc aagatttggc
     4921 gcaaatcgcc aaagcggtgg aattgtataa tattgattat gggcgttatc agattttgaa
     4981 cagcgggtgg ggaagcgata ccggcacggg ttgcggatgc ggttggttca gcgctgaagg
     5041 aggcgcttac gggcgctcca ttgcgcgggc gcttgttgag gacggcggca aattatccat
     5101 ccttgtaaaa gatccgaccg gcggaacatc ttgtagcacg accgccggtt atgcctatat
     5161 gctttatctg cgcggaagcg gtttttatgt ttatgccaag cttgaaaaac ccaatgccgc
     5221 cgatttggcg acttgcgatg ccaatgccca gtgcccgacc aattacggca tgaattatgc
     5281 cgtcggccat cgatagagtt tgtcgcggca attattttat ctggcgcaag aaatcttggg
     5341 cgagggcgac ggtgtcgccg gtggcggtgt agaagttttt ccagaccacg gcgatgtcgg
     5401 cgctgttgaa aaacgctcgc tggacttcgc ggggcgtgtc gccgccggca atcgccagca
     5461 taatgtcata attgcccttg acgcgctgaa tttcgtgata ggggatttgt ttttcccggt
     5521 tgaaaatttc ttcgtccacg ccgcggtgca aaacaacaac cgcgggctgt ttttttaatc
     5581 ggcgcaaaac attcagggaa tattccacgt tcatcatatc aatcattgcg tcaatgccca
     5641 ggttttcgca ttcggcgatg aatgaattca aggtttctat cggcgcctgg cccatggcaa
     5701 ccgccgcgga tgcgccggca ccggcggcca tttgcgcctc ggtggcgcca cggtcaatgg
     5761 tttttagatc cgccacaaca taagccggcg cgttgatctt cgcgctgtcc aaccgttttt
     5821 gccaccaata attcaagcgg gacacggcgg tgatgccgtg ttccttaatc agcggcgtgc
     5881 cggcttcaat aatgatgcgg tcgctggcgg gtaggcgcgc gatgattttt tgcgcgtcgg
     5941 ccagatcgcc gttgagcgcg atttgcaagt atcttttttt accgtttagt tttgccatac
     6001 ctcattataa cacattaagg gatgcaatta aaaatttgtt gatttggagt aaaaattggg
     6061 caaaagcgtg aaaaataggt ttaaaatgcg gattttcacg attatatcgg ctaaaatcgt
     6121 gaactggcgg gtgttgttgc ggttctctta atatggtata gtccaaatat aggggcatac
     6181 catattaagg cttatactga aactatggca tatttgaatt cgcagttaca aaaaagaatt
     6241 gatgaaaa
//
LOCUS       DAOQWE010000039         6215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_12934_length_6215_cov_6.672890, whole genome shotgun sequence.
ACCESSION   DAOQWE010000039 DAOQWE010000000
VERSION     DAOQWE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12934_length_6215_cov_6.672890"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1746)
                     /locus_tag="PKK37_02165"
     CDS             complement(64..1746)
                     /locus_tag="PKK37_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNR81214.1"
                     /translation="MELWAYPYLFYYLGMFAFWMLAMHRQILFWLYLWQLKEYHPGRF
                     LAHFETRKGKSIFLNPLFLGKIAALLFFAGYYYFVHKIGLIIGLALVLYLLQGARSLW
                     GILCRNLIHPELTQKSVFLIGAAHFVVFGLGFAIFGLLLGEMSLIHFAASAFCLLAID
                     ILMPLIISLVVLGLQPLTIWKKNKILNQAREIIKGRTDLSVIGIAGSYGKSGVKELLA
                     HILSQKYKVLKTRANQNTEMGVSQTIINDLKPEHQIFVCEIGAVHKGKIAQTAGIVNP
                     KIGILTGINQQHLGVFGSQKNIVDAKFELLGALPENGTAILNFTSPLVCENFAAKKDE
                     ISAQKIIFAGNDIFASDIQTKIDNLSFILNYQDQKVKIDTNARGAWMAETIILSIAGA
                     ASAGMELAAVAAIVNQTDFTPFNLELKRVGPFLTKKGPTLFEEINIINSTYSANSDGV
                     MAHLDYLKLWSGKKAIIMPCLIELGKESKKIHFEIGQKIAAVCDLAIIATKDCFKEIK
                     AGALSAGMKPENIIFSDKPKKISELLQTRLTAGDILLLEGRLPQEIIREIAG"
     gene            complement(1823..2644)
                     /locus_tag="PKK37_02170"
     CDS             complement(1823..2644)
                     /locus_tag="PKK37_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNR81215.1"
                     /translation="MEERKIIIDQLSINCRFFGGNDKPAVLVLHGWGVGSEAWVEAAV
                     MLAQEDYRVIVPDMPGFGKSEVPEKPWTVDDYVEWVKDFADKLGMDRFILIGHSFGGQ
                     VAAKFCAIRPNKVSKLVLCAAAVIRKPRLGSRQILAKYLAKTKVIFQHLPLGIYPVLR
                     KIVYRIAGTRDYSQLQGVMAQTFLNVVGESVIESARQIKTPTLIVWGDKDRETPIEDA
                     HEINKAIAGSRLEIIPGAGHKLHRTHPQELAKIICQFLMPNIKIQMSNKAQNQNV"
     gene            complement(2809..3228)
                     /locus_tag="PKK37_02175"
     CDS             complement(2809..3228)
                     /locus_tag="PKK37_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HNR81216.1"
                     /translation="MEFGAYEMKRVLVFGTFDGLHPGHINFFQQAKKLGDELIIVVAR
                     DATVQKVKGRPPRKNENARLSDARNAGIAGEVLLGNLGDPYAIIKQIKPDIIALGYDQ
                     TSFTANLESELKKAAISVKIVRLKPHKPEIFKSSLIK"
     gene            complement(3332..4642)
                     /gene="serS"
                     /locus_tag="PKK37_02180"
     CDS             complement(3332..4642)
                     /gene="serS"
                     /locus_tag="PKK37_02180"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNR81217.1"
                     /translation="MLDIKFIRQNRKIVEDACKKKNVKCDIGRLLDLDEKRVKAMQEV
                     EAVAAEKNKASKAIPLAKDQIERDAIINAMKALDQNTGNLEKRAKELEDEFHDLMLEI
                     PNIPSEDSPEGKDDSGNVEVETWGKIPKFDFEIKDHIQLGKELDLIDVEAGVKTGGFR
                     GYYLKNEAVFLHFAVFWHALNLLREKGFTMFVPPTILREFALVGSGHFPAGKDEVYQI
                     ANPGKIESGEEAKEPSYLGGTAECALLAYHAGKILNEKDLPVKMCGISQCYRSEVGSY
                     GKDAKGLYRLHEFMKVEQVVICKNDLKESNRWLETMREISQTILKDLELPHRVLAICT
                     GDMGAGKHKMYDIETWMPSRNAYGETHSDSNLTDWQARRLSTRYRDKDGNIQYPHMLN
                     NTVVASPRILIAILENYQQKDGSIAIPKVLQPYLGGMTKITSKK"
     gene            complement(4755..6212)
                     /gene="lysS"
                     /locus_tag="PKK37_02185"
     CDS             complement(4755..6212)
                     /gene="lysS"
                     /locus_tag="PKK37_02185"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNR81218.1"
                     /translation="MDELRNERLKKLKNLRSAGIWPYPGETKRTHAIGAALSDWKNLE
                     DAQKEIIVAGRLRSMRGHGGMLFGDIQDGTGKIQILLKKDGLGEKSYQFFADNFDVGD
                     FVQIRGTALTTKRGEKTVLAVDYKMLAKALRPLPDKWHGLQDTEERYRKRYLDLIFNE
                     DVRKKFVIRTEIVRAIRNFLDEHGFMEVETPILQTIYGGATAKPFKTYFNAYDMDVFL
                     RIAPELFLKKLLVGGFEKVYEIARNFRNEGVDKQHNPDFTMLEFYWAWSDYRQLMKFT
                     EEMMAYILQKVLGTAKITYQGNEIDFAAPWPRVEYLDLLKKEAKIDYRASSREGLAVK
                     AKELGVEIDKNAGKAEIADEIYKKYCRPKIIPPTFLIHYPKESKPLAKSVEPDGPIAA
                     NFQLLVNGFEVINAFSEQNDPIEQAARLKEQEELFKQGFQEAQRTDTDFIEALEYGMP
                     PCAGLGMGIDRLAALLTDSNALREIILFPLMKPKN"
BASE COUNT         1572 a         1572 c         1291 g         1780 t
ORIGIN      
        1 ccataatcgt ttattttagc cgtattttta tcttatctca accattgaca aaaccaacaa
       61 aaactatccg gcaatttcgc ggatgatttc ttgcggcagg cggccttcaa ggagcaggat
      121 atcgccggcg gtgaggcggg tttgcaaaag ctcgctgatt ttttttggct tgtcggaaaa
      181 aatgatattt tcgggtttca taccggccga tagcgcgccg gcttttattt ctttgaaaca
      241 atctttggtg gcaataatcg ccagatcgca aactgcggca atcttttggc cgatttcaaa
      301 atggattttt tttgattctt tccccaattc aatcaagcac ggcataatta tcgctttctt
      361 tcccgaccaa agcttcaaat aatccaaatg cgccatcacc ccgtcggaat tggccgaata
      421 ggtagaatta atgatattga tttcttcaaa aagggtcgga ccctttttag ttaaaaaagg
      481 tccgaccctt tttagctcaa ggttgaaggg ggtgaagtcg gtttgattga caatcgcggc
      541 aactgcggcc aattccatgc ccgcgctggc cgcgccggca attgacaata tgattgtctc
      601 cgccatccat gcccccctcg cattggtatc tatttttact ttttgatctt gataattaag
      661 aataaacgat aaattatcaa tttttgtttg aatatccgac gcgaaaatat catttccggc
      721 aaaaattatt ttttgcgcgc tgatttcatc ctttttggca gcaaaattct cgcaaacaag
      781 cggactggta aaatttaaaa tcgccgtgcc gttttcgggc aaagctccca atagttcaaa
      841 tttggcgtca acaatatttt tttgactgcc aaacacgccc aagtgctgtt ggttaatccc
      901 cgtcaagatg ccgattttgg ggttgacgat gccggcagtt tgcgcgattt ttcctttgtg
      961 caccgcgccg atttcgcaga caaaaatttg atgttcgggc ttcaaatcat tgatgatggt
     1021 ttgcgacacg cccatttccg tgttttggtt ggcccgggtt tttaaaactt tatatttttg
     1081 cgacaaaatg tgcgccagca gttccttgac gccgctcttg ccgtaactgc cggcgatgcc
     1141 gatcaccgac aaatccgttc tgccttttat tatctcccgc gcctgattca gaattttatt
     1201 ttttttccaa atggtaagcg gctgaagccc caaaaccacc aaagagatta tcaatggcat
     1261 taaaatgtca attgccagca ggcaaaacgc agaggcggca aaatgaatca ggctcatttc
     1321 gcccaaaagc aaaccgaaaa tcgcaaaacc taaaccgaaa acaacaaaat gcgccgcgcc
     1381 aattaagaaa accgattttt gcgtgagttc cggatgaatt aaattgcgac acaagatgcc
     1441 ccaaagagag cgcgcgcctt gcaataaata caacaccagc gccaagccga tgatcaatcc
     1501 gattttatgg acaaaataat aataaccggc aaagaacaac aaggcggcaa ttttccccaa
     1561 aaaaagcgga ttcaaaaaaa tacttttgcc tttccgggtt tcaaaatgcg ccaaaaaacg
     1621 ccccggatga tattctttca actgccacaa ataaagccaa aacaaaatct gccggtgcat
     1681 tgccagcatc caaaaagcga acattcccaa atagtagaaa agataaggat atgcccagag
     1741 ttccataatt ttataaatcc caatttcaaa gctcaaatgt caaacaaaat ccaaagttca
     1801 aaatccaaaa gagtctcaat ttttagacat tttgattttg tgctttgttt gacatttgga
     1861 ttttgatatt tggcattaag aattggcaaa ttattttggc caattcttga ggatgcgtgc
     1921 ggtgcagttt atgtcccgcg ccgggaataa tttccaatcg cgaaccggca atcgctttgt
     1981 ttatttcatg cgcatcctct atcggagttt cgcggtcttt atcgccccaa acaatcaaag
     2041 tcggcgtttt gatttgtctt gcggattcaa tcacactttc gccgacaaca ttcaaaaatg
     2101 tctgcgccat cacaccctgc aactgcgaat aatcgcgggt gccggcgatg cgataaacaa
     2161 tcttgcgcaa aactggatat atccccaatg gaagatgctg gaaaataacc ttggttttag
     2221 ccagatattt cgccaaaatc tggcgcgaac ccaggcgcgg cttgcgaatc accgcggcgg
     2281 cgcacaaaac caatttgctc actttatttg gacgaatcgc gcaaaacttg gccgccacct
     2341 gcccgccaaa cgaatgccca attagaataa accggtccat ccccaactta tcggcaaaat
     2401 ctttcaccca ttccacataa tcatcaactg tccaaggttt ctccggcacc tcgcttttac
     2461 caaacccggg catatccggc acgattaccc gataatcttc ctgcgcgagc attaccgcgg
     2521 cttccaccca tgcctccgac ccaacacccc acccatgcaa caccaaaact gccggtttat
     2581 cgttcccgcc gaaaaatcta caatttattg ataattgatc aatgataatt tttctctcct
     2641 ccattgacaa aatcggtaaa aattgcttgt tttaaatcag catgttcgaa aaatacaatt
     2701 tggaagctgg ggttggcggc atattatttg ggattttttt ctttgccgga atggttgtaa
     2761 ttacttgcat gtttgtgggc gcatttcatt tttcgaaata gcccattttt attttattaa
     2821 gctggattta aaaatttcgg gtttgtgggg tttgaggcgc actatcttta ccgatattgc
     2881 ggcttttttt aattcggatt caagattggc cgtaaaggag gtttggtcgt agccaagggc
     2941 gatgatatcg ggttttatct gcttgatgat tgcgtagggg tcgcccaaat tacccaacaa
     3001 aacctcgccg gcgatgccgg cgtttctggc atcggacaat cgcgcatttt catttttccg
     3061 cggcgggcgg cctttcactt tttgcaccgt ggcatcccgc gccaccacga tgatcaattc
     3121 atcgccaagt ttcttcgctt gttgaaaaaa attgatatgg ccgggatgca ggccgtcaaa
     3181 tgtaccgaaa accaatactc gtttcatctc gtaagctcca aactccaaat cacaaattcc
     3241 aaataatatt caaaatttaa tatttaaaat ttaaaattta gatatttgaa tttatttggg
     3301 atttgatgct tggtgcttgg aatttccaaa atcacttctt tgaagtgatt ttggtcattc
     3361 ctcccagata tggctgtaaa actttcggaa tggcgattga gccgtctttt tgctggtaat
     3421 tttccaaaat ggcgatcaga atccgcgggc tggccaccac cgtgttattg agcatatgcg
     3481 gatattggat gttgccgtcc ttgtcgcgat agcgcgtgct caagcgccgc gcctgccaat
     3541 cggtgaggtt ggaatccgaa tgcgtttcgc cgtaggcgtt gcgggacggc atccaggttt
     3601 caatatcata cattttgtgc tttcccgcgc ccatatcgcc ggtgcaaatc gccaaaacac
     3661 gatgcggcag ttccaaatcc ttgagaatgg tctgtgaaat ctcgcgcatc gtttccagcc
     3721 accgatttga ttctttcaag tcgtttttgc aaatcaccac ctgctccact ttcataaatt
     3781 cgtgcaggcg gtacaatccc ttggcgtcct tgccataact gccaacctcg gaacgatagc
     3841 actgggaaat gccgcacatt ttgaccggca aatctttttc attcaaaatt tttccggcat
     3901 gataggccaa taatgcgcat tcggcggttc cccccaaata cgacggctct tttgcctcct
     3961 cgccactttc aatttttccg ggattggcga tttgatacac ttcgtctttg ccggcgggaa
     4021 agtgtccgga acccaccagc gcgaattcgc gcaggatcgt gggcggcaca aacatcgtga
     4081 atcccttttc gcgcaacaag ttgagcgcgt gccaaaacac cgcgaaatgc aggaagaccg
     4141 cttcgttctt gagataatag ccgcgaaaac cgccggtctt gaccccggcc tcaacgtcaa
     4201 tcaaatccaa ttcttttccc agctggatat ggtctttgat ttcaaaatca aatttgggga
     4261 ttttgcccca agtctccacc tccacgttcc ccgaatcatc tttgccttca ggagaatctt
     4321 cggaaggaat attgggaatc tccagcatta aatcatggaa ttcatcctcc agttccttgg
     4381 ctcttttttc cagattgccg gtgttttgat ccagcgcctt catcgcattg atgattgcgt
     4441 cgcgctcaat ttggtctttt gccagaggga tcgcttttga cgccttgttt ttttccgcgg
     4501 ccacggcttc aacttcttgc atagccttga cgcgtttttc atccaaatcc agcaatctgc
     4561 cgatgtcgca cttgacattt ttctttttgc aggcgtcttc aacgattttt ctgttctggc
     4621 gaatgaattt aatatccaac atagataata agctccaagt tccaaattac aaatctcaaa
     4681 taatattcaa ctcctaatat ttaaattttg aatattggaa tttactttgt aatttggtac
     4741 ttggtgctta gaatttaatt tttaggtttc atcaacggga acaaaattat ttcccgcagg
     4801 gcgttggaat cagtcaacag cgccgccagc cggtcaatac ccatgcccaa accggcgcac
     4861 ggcggcatac catactccag cgcttcaata aaatcggtgt cggtgcgctg agcctcctgg
     4921 aacccctgct tgaacaactc ttcctgctct ttcagacgcg ccgcctgctc aatcggatcg
     4981 ttttgttcgg aaaacgcgtt gataacctca aatccgttga ccagcagttg gaaattggcc
     5041 gcgatcgggc cgtccggttc aacacttttg gccagcggct tggattcctt gggatagtgg
     5101 atcaaaaagg ttggcgggat aattttcggc cggcagtatt tcttgtagat ctcgtcggcg
     5161 atttcggcct tgcccgcgtt tttatcaatt tccacgccca attctttggc cttgacggcc
     5221 aatccctcgc ggctggaagc ccgataatca attttggcct cttttttcag caagtcaaga
     5281 tattccaccc gcggccaagg cgcggcaaaa tcaatttcgt tgccctgata ggttattttt
     5341 gccgttccca aaactttttg caaaatataa gccatcatct cttcggtgaa tttcatcaac
     5401 tgcctgtaat cggaccacgc ccaatagaac tccagcatcg tgaaatccgg attgtgctgt
     5461 ttgtccacgc cctcattgcg gaaattgcgc gcgatctcat acaccttttc aaagccgccc
     5521 accaaaagtt ttttcaaaaa caattccggc gcgatgcgca gaaacacgtc catatcatag
     5581 gcgttaaaat atgtcttgaa cggtttggcc gtggcgccgc cgtagatcgt ttgcaatatc
     5641 ggcgtttcca cttccataaa tccatgctca tccaaaaaat tccggattgc tctgacgatt
     5701 tcggttcgga tcacgaattt tttcctgaca tcctcgttga aaatcaagtc caggtagcgt
     5761 ttgcggtaac gctcttcggt atcctgcagg ccgtgccatt tgtccggcaa ggggcgcaga
     5821 gccttcgcca acatcttgta gtcaaccgcc agcaccgttt tttctccgcg cttggtggtg
     5881 agcgccgttc cccggatctg cacaaaatcc cccacgtcaa aattgtccgc gaagaactga
     5941 taggactttt cgcccagccc gtcttttttg agtaaaatct gaattttgcc ggtgccgtct
     6001 tgaatgtcgc caaacaacat tccgccgtgg ccgcgcattg accgtaaacg gccggcaacg
     6061 ataatttctt tttgcgcgtc ttccagattt ttccaatccg aaagcgccgc gccgatcgcg
     6121 tgcgtccgtt ttgtttctcc cggatagggc caaatgcccg cggaccgcaa atttttcagt
     6181 tttttcagcc tctcattccg aagctcatca atatt
//
LOCUS       DAOQWE010000040         6161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_13057_length_6161_cov_5.963315, whole genome shotgun sequence.
ACCESSION   DAOQWE010000040 DAOQWE010000000
VERSION     DAOQWE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13057_length_6161_cov_5.963315"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKK37_02190"
     CDS             <1..317
                     /locus_tag="PKK37_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81219.1"
                     /translation="VDAKIRFVDENSQGPEISTPLPSGNREESDLILSVQTDEDATCK
                     FDRQDAKYGEMKYSFGATGQKLHQQKVCDLSEGEFTWYVRCKGQSGTNDGSSAIRFKV
                     DD"
     gene            427..1578
                     /locus_tag="PKK37_02195"
     CDS             427..1578
                     /locus_tag="PKK37_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81220.1"
                     /translation="MPAALPLALVIAMPLAAAAFFDSSYTAPGVNYTVRDGTAYIGYN
                     SGRGDAVQSTSLGKNVGSFLGGPLGAIFKKENGREMVDGDVVTISEGEFLGWMEATGA
                     GVFVDPLFKGADVVGAADNFTERLREYFSKNPSENGKTILQYYSDTPESFADLEKAFK
                     NSAAKTEITTDIKKLSDLKESILPFSVATPTEGEPKFHSKEYYMKMGYHEEMAAELAA
                     KNAAAAVDYDAIAKKYGADAAEVVRNAVEEDGCGINEDGFITYNGAPSTSYNNAFTAA
                     ARAQSGSADAAAAPGSFEDSRKEYIAGQISEEEYIARVEVERGVRYDKKYNNFKINGT
                     VNTVDILATEVLTGHIVFSDDSSACAVFDDPSMDPYAPRGIDGSGTGLL"
     gene            1646..>2562
                     /locus_tag="PKK37_02200"
     CDS             1646..>2562
                     /locus_tag="PKK37_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81221.1"
                     /translation="MNPNHFCRIDKKFLFGFAALAFAGLCLWQGGLFSAENAAAKTSA
                     SFSDYVGENIRMSGGITYIRYTGVRPVSNSPLLSNLMRAAGTIIDGEGGWVSEEDYAM
                     IVAAVVDVLFENASGNFLDNFTEKLVTSFGSLPENKGQKLAERLNKYYDTHPEEMKSL
                     GTAFAVAKMKVDSEGQTKEGLMEEYAWGNAIEEGLTTQRYSDEEIKGMGYSDKQTDFI
                     KETLEQHPDWSVDDEYFYVDRDGNGKRNSGDLVMGGDDGYALTVANETLDGRPTSDPS
                     KSACEWLGDSSMDPYGSGGINEEGTGLLGS"
     assembly_gap    2563..2662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2663..4337
                     /locus_tag="PKK37_02205"
     CDS             <2663..4337
                     /locus_tag="PKK37_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81222.1"
                     /translation="SGNVACTAYDYSAWSVCAGGQQTRTITGQSPAGCTDTSSAELTQ
                     TCTVSGNVACTAYNYSAWSVCAGGQQTRTITGQSPSGCTDTSSAELTQTCTMPAGACV
                     SYTYSDWSACADGRQTRTVVSRSPEGCDAASAILEQTCLTPCVSYNYGDWSACVAGQQ
                     TRAIISRSPENCDASSAVFTQPCVAPESPVVENTTNAVQSDSSAALSVITDRPADCQY
                     NSAGGFSFGAGTDFSATGSYFHNAALANVTAGEKTYYVICKDKATGGLSEIIKIDFRV
                     ESSAAPDETCAELSANDRKNNAQRSWSDTATADSVYLWQSIRLGREESFDKVDWYAGY
                     QFTPQRDGRANQVCGYFKSGSTNKVSIYNGSYNELGGAQISGTGGWKCVDIAPVEIKT
                     DKRYYVIARIEDGPVYLDNKPGMLPRSAANALVEAGIRQSAAEGAFGNKVIKYDHSVL
                     GLVDVRIAYTPETATGPIIASLSPQGTVNTADVRLAAESSAAADCRFGRDDIGYAQMP
                     YEMSEISDGSFGKKVCGLENGAYTFYVRCRSSAGQENNFSKTIQFNVAQ"
     gene            4355..5986
                     /locus_tag="PKK37_02210"
     CDS             4355..5986
                     /locus_tag="PKK37_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81223.1"
                     /translation="MFSLKIDSGFQKGMAAAAVALLAAALAPAAFAAGPILKNINPQG
                     MVTSSSVKITLDTDDLARCRYSTGDTDYESMGNDLYTPDGLYHSGNIGTLDDGSYTYY
                     VRCKDFEGNANSVSAVVNFNVGEVGCLGDNCGTVTPPPASTVPPALSNFLPSGTVSTK
                     YVVLSVSTDKSASCRYSWYDKPYDEMTLAFTSNNGLYHVASATLGNGGNYNYYVRCKD
                     NAGNVNSPAGKISFYYYVYVAPKATAPAVTTPAAPSDTAAPSISGLSPSGTVSAKEVS
                     LSFDTDETATCRYGESDVEYGSMTENFGAAGTRHTKTITLDDPGEYTYYVRCADSAGN
                     ENAASEQISFTYSLPEGPKVSNLEPSGGVVYQNSVALIASTDKAASCRFAESDTDYEQ
                     MGNFFDTNDGLFHQAFVDLPDYGYYSYYVRCTDKEGVPGSRAETINFEYQNPNPEPEP
                     ETVAPEPVVCEQIQIGNKNGECDPTTDCICDPDCPVEGQDADPDCAGVSQPSSGGGGG
                     WAILLLIGLILLIIIVIIIIIARRKSAEEEDVELP"
BASE COUNT         1455 a         1505 c         1739 g         1362 t
ORIGIN      
        1 tggtggatgc caaaatcaga tttgttgacg agaattcgca gggtccggaa atatcaacgc
       61 ctttgccttc cgggaatcgc gaagaaagcg atttgatctt gtcggtgcaa accgatgagg
      121 acgcgacgtg caaatttgac cgccaggatg ccaaatacgg cgagatgaag tatagcttcg
      181 gcgccacggg gcagaaattg catcagcaaa aagtttgcga tttaagcgaa ggggaattca
      241 cttggtacgt gagatgcaaa gggcagtccg gtaccaatga cgggtcgtcc gcgatccggt
      301 tcaaagttga cgattagctg tgctccggca gaggcccgtc cgctttctga agcgccggcg
      361 gcgcctccgc cgtgccaatg aagaaagacg atgtttattt ttccaaaatt ttgtcggaaa
      421 aaaagattgc cggcggcact gccgctggcc ttggtgattg cgatgccctt ggccgccgcc
      481 gcgttttttg attcgtccta taccgctccg ggcgttaatt acacggtgcg cgacggcacc
      541 gcctatatcg gctataactc cggccgcggc gatgccgtgc aatcaacaag tttggggaaa
      601 aacgtgggat cgtttcttgg cgggcctctg ggggcgattt tcaaaaaaga gaacgggcgc
      661 gagatggttg acggagacgt ggtaacgata agcgaaggcg aatttttggg atggatggaa
      721 gcgacgggcg ccggcgtttt cgtggacccc cttttcaagg gcgccgatgt ggtgggcgcg
      781 gcggataatt tcacggaacg tctgcgggaa tatttttcaa agaacccttc cgaaaacgga
      841 aagaccatat tgcagtatta ttccgacacg ccggaaagtt tcgccgattt ggaaaaggcg
      901 tttaaaaatt ccgccgccaa gactgaaatc accactgaca tcaaaaaact gagcgattta
      961 aaagaatcca ttttaccgtt ttcagttgcc acgccaacgg agggcgagcc gaaattccat
     1021 tccaaagaat attatatgaa aatgggatac cacgaagaga tggcggcgga gctggcggca
     1081 aaaaacgcgg cggcggcggt tgactatgac gcgattgcca aaaaatatgg cgcggatgcc
     1141 gcggaggtgg tgagaaacgc ggtggaggaa gacggatgcg gaataaacga ggacggattt
     1201 atcacttaca acggcgcccc cagcacaagt tataacaacg ctttcaccgc ggcggcgcgc
     1261 gcccaatccg ggtccgcgga cgcggcggcg gcaccgggga gctttgagga ttcgcgcaaa
     1321 gaatatatcg cgggccaaat ctcagaggag gaatatattg cccgagtgga ggttgaacgg
     1381 ggggtccggt atgacaaaaa atacaataat ttcaagatca acggcaccgt taacacggtt
     1441 gacattttgg ccacggaagt gctcaccggc cacattgtgt tttcggacga ttcgtcggcc
     1501 tgcgcggtgt ttgacgatcc gtcaatggac ccctacgcac cacgcggcat tgacggttcc
     1561 ggcaccggcc tgctttgatt ggccgccccg cgtccgcaaa attggcaaat tttaaaatta
     1621 aatcataatt tacgaaaaaa acaatatgaa tcctaatcat ttttgccgga ttgacaaaaa
     1681 atttctgttt ggttttgccg cgctggcgtt tgccggcctg tgcctctggc agggaggttt
     1741 gttttccgcc gaaaacgccg ctgcgaaaac ttccgcttct ttctccgact atgttggtga
     1801 aaacataagg atgagtggcg ggataactta tatccgttat accggcgtta gacccgtatc
     1861 aaacagtcct ttgttgagca atttgatgcg ggccgccggg actatcattg atggtgaggg
     1921 tggctgggtg tcggaggagg attatgcgat gattgttgcc gccgtggttg atgtgctttt
     1981 tgaaaatgcc tccggtaatt ttttggataa ttttactgaa aaattagtga cttcttttgg
     2041 ttctttacca gaaaataaag ggcaaaaact tgcagagaga ttgaataaat attatgatac
     2101 tcatcctgaa gagatgaaga gtttggggac ggcttttgcg gttgcgaaaa tgaaagtgga
     2161 ctcggaaggc caaacaaaag aaggactaat ggaagagtat gcatggggga atgccataga
     2221 ggaagggctg acgacgcaga ggtattcgga tgaagaaatt aaagggatgg ggtattctga
     2281 taagcaaaca gactttatta aagaaacatt ggagcaacat cccgattgga gcgttgatga
     2341 cgaatatttt tatgttgata gggatggtaa tggcaaacgc aactcgggag accttgtgat
     2401 gggcggtgat gatggctatg ccttaactgt tgccaatgaa acgttggacg gcaggccaac
     2461 cagtgatccg tcaaagtcgg cttgtgagtg gcttggcgat tcgtcaatgg acccctacgg
     2521 ttccggcggg ataaacgagg aggggacggg gcttttgggt tcnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnttcgggca atgttgcctg caccgcctat gattacagcg
     2701 cgtggagcgt ttgcgccggc ggacagcaaa cccgcaccat caccggccag tcgccggccg
     2761 gttgcaccga tacttcttcg gcggaattga cgcaaacctg cacggtttcg ggcaatgttg
     2821 cctgcaccgc ctataattac agcgcgtgga gcgtttgcgc cggcggccag caaacccgca
     2881 ccatcaccgg ccaatcgccg tcgggttgca ccgacacttc ctcggcggaa ttgacgcaaa
     2941 cctgcacaat gccggcgggc gcttgcgttt cctacactta cagcgattgg agcgcgtgtg
     3001 ccgatggccg gcaaacccgc accgttgttt cgcgctctcc cgaaggttgc gacgcggcct
     3061 cggcgatatt ggaacaaact tgcctgacgc cttgcgtttc ctataattac ggcgattgga
     3121 gcgcgtgcgt tgccggccag cagacccgcg ccatcatttc gcgctctccc gaaaattgcg
     3181 atgcctcttc ggcggtgttc acccaaccgt gtgtcgcgcc ggaaagcccc gtggtggaga
     3241 acacgaccaa tgccgttcaa agcgattcct cggcggcgct ttcggtgatc accgatcgtc
     3301 ccgcggattg ccaatataat tcggcgggcg gattttcttt cggcgcggga acggactttt
     3361 ccgccacggg aagctatttc cacaatgccg cgcttgccaa tgttaccgcc ggtgaaaaaa
     3421 cctactatgt catttgcaag gacaaggcca ccggcggatt gagcgaaata atcaaaattg
     3481 attttcgcgt tgagtcttcc gccgctccgg acgaaacctg cgccgaactt tccgccaacg
     3541 atcgcaagaa caacgcccag cgttcatggt cggacaccgc gacggcggat tcggtttatt
     3601 tgtggcagtc aatcagactc gggcgggaag aaagttttga caaagttgac tggtatgctg
     3661 gctatcagtt cacgccccag agggatggcc gggccaatca ggtttgcggt tatttcaaga
     3721 gcggatcaac caataaagtt tcaatttaca acggctcata caatgaatta gggggcgcgc
     3781 aaatttcggg caccggcggg tggaaatgcg ttgatatcgc tcccgtggag ataaaaaccg
     3841 acaaacgcta ttatgtgatc gcccggattg aggacgggcc ggtttatctt gacaacaagc
     3901 cggggatgtt gccgcggagc gccgccaacg cgctggttga ggcgggaatc cgccagtccg
     3961 ccgccgaagg cgccttcggc aataaggtga taaaatatga ccattcggtc ctcggcttgg
     4021 tggacgtgag aatcgcttat actccggaaa ccgccaccgg tcccataatc gcttcgctta
     4081 gcccacaagg cacggtgaat accgccgacg tccggctcgc cgcggaatcg tctgccgccg
     4141 cggattgccg cttcgggcgc gacgatatcg gatacgcgca gatgccctat gaaatgtccg
     4201 aaatttccga cggcagtttc ggcaagaaag tttgcgggct ggaaaacgga gcatacactt
     4261 tctatgttcg gtgccggtcg tcggccggcc aggagaataa tttttccaaa acgatccagt
     4321 ttaacgtggc gcaataaata atatgaaaat gcctatgttt tcgcttaaaa tagattccgg
     4381 cttccaaaag ggaatggccg ccgccgcggt ggcgcttttg gcggccgcat tggcgccggc
     4441 cgcgttcgcc gccggtccga tattgaaaaa tatcaatccg cagggaatgg tgacttcttc
     4501 cagcgtcaag atcactttgg acaccgatga tctcgcgcgt tgccggtatt ccaccggcga
     4561 cacggattac gaaagcatgg gcaatgatct ttacactccc gacggcctct accattcggg
     4621 gaatatcggc actttggatg acggaagcta cacttattac gtgcgttgca aggactttga
     4681 gggaaacgcc aacagtgtga gcgcggtggt caattttaac gtgggcgagg tgggttgcct
     4741 tggcgacaat tgcggcaccg tcacgccccc gcccgcgtcc accgtgccgc cggcgctttc
     4801 caattttttg ccctcgggca cggtgagcac caagtatgtg gtgctttcgg tttccaccga
     4861 caaatcggcg tcgtgccgtt acagctggta tgacaagcca tatgatgaaa tgacgctggc
     4921 gttcaccagc aacaacggcc tttaccatgt ggcgtcggcg accttgggca acggcggcaa
     4981 ttacaattat tacgtgcgct gtaaggacaa tgccggcaat gtcaattctc cggcggggaa
     5041 aatttcgttc tattattacg tttatgtcgc gcccaaagcg acggcgccgg cagtgacgac
     5101 gccggcggcg cccagcgata ccgcggcgcc gtcaatttcg ggattgtcgc cctcgggcac
     5161 ggtgagcgcc aaagaagttt ctttgtcgtt tgataccgac gaaacggcta cttgccgcta
     5221 tggcgaaagc gatgttgaat atggatcaat gaccgagaac ttcggcgccg ccggtacccg
     5281 ccataccaaa acaatcacgc tggacgaccc cggagaatac acatattatg tccggtgcgc
     5341 cgacagcgcc ggaaacgaga atgcggcttc ggaacaaatc agctttacct actccctgcc
     5401 ggaagggccg aaagtttcca atcttgaacc gtcgggcggc gtggtttatc aaaacagcgt
     5461 ggcgttgatc gcgagcaccg acaaagccgc ttcgtgccgt ttcgcggaaa gtgataccga
     5521 ttacgagcag atgggaaatt tttttgacac caatgacggg ctgttccatc aagctttcgt
     5581 ggatttgccg gattacggat attattctta ctatgtgcgt tgcaccgaca aggagggggt
     5641 gccgggaagc cgcgcggaaa cgatcaattt tgaatatcaa aatcccaatc cggagcccga
     5701 gcccgagact gttgcgccgg agccggtggt ttgcgagcag attcagatcg gcaacaaaaa
     5761 cggagagtgc gatccgacga cggactgcat ttgcgatccg gattgtccgg ttgaaggaca
     5821 ggacgcggat cccgactgcg ccggcgtcag ccaaccttca agcggcggag gcggcggttg
     5881 ggcgatatta cttttgatcg gattgatcct gctgataatc attgtcatta ttatcatcat
     5941 cgcgcggcgg aaaagcgccg aagaagaaga cgtggaactc ccataaaaac acgtaaagca
     6001 aaaatcaaga acgcgcctcc ggcgcgttct tgatttaatg gtttttaaag gggtccgttt
     6061 taaagggtcc gacccttttg aaaagggtcg gacccttttg aaaatcttat gaaagattgt
     6121 aggagatttt ttttatctta tagataatct ttccgtttcc g
//
LOCUS       DAOQWE010000041         5930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_13712_length_5930_cov_7.396426, whole genome shotgun sequence.
ACCESSION   DAOQWE010000041 DAOQWE010000000
VERSION     DAOQWE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13712_length_5930_cov_7.396426"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PKK37_02215"
     CDS             <1..491
                     /locus_tag="PKK37_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81224.1"
                     /translation="NFLNSLDFSLQILVQSRKLNITSYLDKLRDLETRQINDLLRTQI
                     VEYRNFINEIVSGGTIMNKTFYVIVPFQPIMLPSLAGAGGKDAAANREKIARAAQEQF
                     GQSKTQLAQRMEFVALGLRRCGLQCVPLNSLEIAEFLWSTYHLEDSERGYYPEIPPEL
                     IQ"
     gene            509..2434
                     /locus_tag="PKK37_02220"
     CDS             509..2434
                     /locus_tag="PKK37_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81225.1"
                     /translation="MALFSSSKKKKRMELPGQELQMKTINIRDIIAPSSIVLASDHFK
                     IGERCGKSFFIFSYPRYLNSGWFTPVINLDTPMDVSFHIHPIDTGTTLKQLRRKLTEV
                     QAEIMERQEKGLVRDPALEIAYQDLEGLRDKLQTSTERMFRLGIYLTVYEKDLAKLRD
                     VETTLRSILESKLIYIKPSLYQQKEGFVSTCPYGIDRIQTYTPMNTSPLSSTFPFVSF
                     DLSANEGILYGTNLHNNSLILFDRFSMENANSVIFAKSGSGKSYTVKLEILRSMMMGV
                     DAIVVDPENEFKTLAEATGGSFLNVSLSSPFHINPFDLPDPREDENPADVIRSNIINI
                     VGLVRILLGGMTPEEDAIIDRALTEVYAAKDITPQTDPKLWAGRTPLMQDLESVLEGM
                     EGADSLVRRIRKFTQGTYADFFNQPTNISMDKPLVVFGIRDMENEFRTSAMYIIMHYI
                     WNKVRSKSRKRILVVDEAWVLMQSEDGASFLYGLCKRARKYWLGVSTITQDVADFMRS
                     NYGQPIITNSSIQILLKQSPATIDVVQKTFNLTDREKGLLLEAEIGEGLFFAGQKHVA
                     MKVVASYVEDQIITTNPEELEKIKRAKEEGRKNAAQAAVKTAVPPEIANRGGGPSKEI
                     PPANSGGPVAAEGTTPG"
     gene            complement(2503..2964)
                     /locus_tag="PKK37_02225"
     CDS             complement(2503..2964)
                     /locus_tag="PKK37_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNR81226.1"
                     /translation="MNDKKITPEEIKKIARDLLAKIDAGAEVTVNASDAGAMEVEIKT
                     NDAAAIIGPNGETLQALRHLLRVMARKITGETVFIDVDVNGYLKKRKDYLRQLALSTA
                     DDAALKNKEISLPPMNSYERRIVHMELSARRDVATESRGCDADRRVVVKPA"
     gene            complement(2973..3683)
                     /locus_tag="PKK37_02230"
     CDS             complement(2973..3683)
                     /locus_tag="PKK37_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNR81227.1"
                     /translation="MNIFDALIYQPLFNVLILFYHWLGDFGFAVIALTVAIRILLYPL
                     AAKAIRVQKATTRIQPQMKDIQKKYKDDKEKQALEMMALWRENKINPLSGFLLLLVQI
                     PVLWGLYRVFWDGFKDESLAMLYGFVPNPGSVDPMFLDHINLAASFPAFAVAAGVLQF
                     VQAKMMMPSVPAGDGKEKSQSEQFAEMMQKQTLYVFPAVTVAIFWALPSALGLYWIAS
                     GIFSVGQQYYILKKNGKN"
     gene            complement(3705..4058)
                     /gene="rnpA"
                     /locus_tag="PKK37_02235"
     CDS             complement(3705..4058)
                     /gene="rnpA"
                     /locus_tag="PKK37_02235"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNR81228.1"
                     /translation="MLPPENRLKKEKEIKRALAGKIAAGGAALSLRAAATDLPAARFC
                     FVVSKRVSKKAPARNLVKRRLRAAAAALMGRITPGFDCTVIARPGAEKKTYRELAAEM
                     EKILAKSRLLAPQSL"
     gene            complement(4084..4218)
                     /gene="rpmH"
                     /locus_tag="PKK37_02240"
     CDS             complement(4084..4218)
                     /gene="rpmH"
                     /locus_tag="PKK37_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNR81229.1"
                     /translation="MSVTYQPKKRKRSKTHGFRERSKTVGGRRVLNSRRAKGRKKLSV
                     "
     gene            4616..>5930
                     /gene="dnaA"
                     /locus_tag="PKK37_02245"
     CDS             4616..>5930
                     /gene="dnaA"
                     /locus_tag="PKK37_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNR81230.1"
                     /translation="METQNANNEKIWRAILAQIQLNVSPAIFATWFKSTSIISIENGE
                     VRIAVPNCLAKEWLEKNSRKQMIVLLREFNTSIREVFFKVQQPPTDKNIFVRARPASG
                     GQLGLDEFKFNKETNLNPKYHFDCFIVGPHNELAHAAAAAAAEKPGQTYNPLFIYGGV
                     GLGKTHLLQAIGNKVAHDFPEKKIRYVPTEKLTTEIINAIRGGTIQNLRAKYLDTAVF
                     IIDDIQFISGKDKTQDEFFYLFNTLYNKNLQIVLSSDRPPKAITELSNRLRSRFEGGM
                     IADISLPDFETRMAILKEKCQIMKIDFSDEILSAIAGTIQNNVRELEGALIKIAAHFK
                     LKKEPPTPEICREILAAINPKKVIGFKKIIQETATFYEIKEKDIISDSRKKEIVVPRQ
                     VVMYLLRNEINASFPFIGRKMGNKDHTTVMHAYKKISSKIKDDASF"
BASE COUNT         1605 a         1535 c         1400 g         1390 t
ORIGIN      
        1 agaatttttt gaattctctg gatttttctc tgcaaattct ggtgcaaagc cgcaagctaa
       61 acatcaccag ttatctggac aaactgcgcg atctggaaac gcgccagatc aacgaccttt
      121 tgcgcaccca aatcgtggaa taccggaatt tcatcaacga gattgtttcg ggaggcacga
      181 ttatgaacaa gaccttctat gtgatcgtgc ccttccagcc gataatgctt cccagtttgg
      241 cgggcgcggg cggtaaagac gcggcggcca accgcgaaaa aatcgcgcgg gcggcgcagg
      301 agcaatttgg acagagcaaa acccagctgg cgcaaagaat ggaattcgtg gcgctgggcc
      361 tccgccgttg cggcttgcag tgcgtgccgc taaacagctt ggaaatcgcc gaatttttgt
      421 ggagcacata ccatcttgaa gattcggaac gcggctacta tccggaaatc cccccggagt
      481 taattcaata gaagtttaaa attgaaacat ggctttattc tcgtcgtcca aaaaaaagaa
      541 gcggatggaa ttgccgggcc aggaactgca aatgaaaacc atcaacatcc gggacattat
      601 cgcgccgtcg tcaattgttt tggcgtcgga tcatttcaag atcggggagc ggtgcggcaa
      661 atcgtttttc atcttttcct atcctcgcta tttgaattcc ggatggttca cgccggtgat
      721 caacctggac acccccatgg acgtatcttt ccatatccac cccattgaca ccggcaccac
      781 gttgaaacaa ttgcgccgga aactcaccga agtccaagcc gaaattatgg aacggcagga
      841 aaaggggctg gtacgcgatc cggcgctgga aatcgcttat caagatttgg aaggactgcg
      901 cgacaaactg cagacctcca cggaaaggat gttccggctg ggaatttatc tcaccgtcta
      961 cgaaaaagat ttggcaaaac tgcgggatgt tgagactact ctgcggtcaa tcttggaatc
     1021 aaaactgatc tatatcaagc cgtcgctcta ccagcaaaaa gagggtttcg tttccacctg
     1081 tccctacgga attgaccgga tacaaaccta cacgccgatg aacaccagcc cgctgtcttc
     1141 aacgtttcct ttcgtttctt ttgacctttc cgccaatgaa ggcatactct acggcaccaa
     1201 cctgcacaac aattcgctga tcctctttga ccgtttttca atggaaaacg ccaacagcgt
     1261 gattttcgcc aaatccggtt ccggaaaaag ctacaccgtc aaactggaaa ttttgagatc
     1321 aatgatgatg ggggtggacg ccatcgtggt tgatccggaa aacgagttta aaactctggc
     1381 cgaagccacg gggggctcgt ttttgaacgt ttctttgtcg tcgccgttcc acatcaaccc
     1441 gtttgacttg cccgatccgc gggaagacga aaatcccgcc gacgtcatcc gctccaacat
     1501 catcaacatc gtcgggctgg tgcgcatcct tctgggagga atgacccccg aggaagacgc
     1561 aatcattgac cgggcgctca ccgaagtcta cgccgccaaa gacatcacgc cgcaaaccga
     1621 ccccaaatta tgggccgggc gcacaccgct gatgcaggac ctggaatcgg ttttggaagg
     1681 aatggaaggc gccgattcgc tggtgcggcg catccgcaaa ttcacccagg gaacatacgc
     1741 ggattttttc aaccagccaa ccaacatctc aatggacaag ccgctggtgg ttttcggcat
     1801 ccgcgacatg gaaaatgaat tccgcacctc ggcaatgtat attattatgc attatatctg
     1861 gaacaaggtg cgctccaaat cgcgcaagcg gatcctggtg gtggacgagg cctgggtgct
     1921 gatgcaaagc gaggacggcg cctcgtttct atatggcttg tgcaagcggg cgagaaaata
     1981 ctggctcgga gtgagcacca tcacccagga tgtcgccgac tttatgcgct caaactacgg
     2041 ccagccgatc atcaccaact cttccattca aatcctgctt aaacaatcgc cggcgacgat
     2101 tgacgtggtg caaaaaactt ttaatctcac cgacagggaa aaggggctct tgctggaagc
     2161 ggaaatcggg gaggggctgt tttttgccgg acaaaaacac gtggccatga aagtggtcgc
     2221 ctcttatgtt gaagaccaaa tcatcaccac caatcccgaa gaactggaaa aaatcaagcg
     2281 ggcgaaagaa gaagggcgaa aaaacgcggc gcaagcggcc gtcaagacgg cggtgccacc
     2341 ggaaatcgcc aatcgcggcg gcggtccatc caaggaaatt ccgcccgcaa acagcggcgg
     2401 accggtggcg gcggagggaa caactcccgg ataaatcctc gccggaggca aagaacaaaa
     2461 aatccgcgct tgaaaacgcg gatttttcat ttttaaaaac ggttaagcgg gcttgaccac
     2521 cacccgccgg tcggcgtcgc acccgcggct ttcggtggcc acatcgcggc gcgcggacaa
     2581 ctccatgtgc acaatccgcc gctcgtaaga attcatcggc ggcagggaaa tttctttgtt
     2641 tttcaatgcc gcgtcatcgg cggtcgaaag cgccagctgg cgcaaataat cttttctttt
     2701 ttttaaataa ccgttaacgt ccacatcaat aaaaaccgtt tcaccggtaa ttttgcgcgc
     2761 catcacccgc aaaagatgcc ggagcgcctg caaggtttcc ccgttgggac cgataatcgc
     2821 cgccgcgtcg ttggttttaa tttccacctc catggcaccg gcatcggagg cattgacggt
     2881 aacttccgca ccggcatcaa ttttcgccag caaatcccgc gcgatttttt tgatttcttc
     2941 gggggtgatt tttttgtcgt tcatattttt tatcaatttt taccgttttt tttcaaaata
     3001 taatattgct ggccgacgga aaaaatcccg gaagcaatcc aataaagccc cagtgcggac
     3061 ggcaacgccc aaaagatcgc caccgttacc gccggaaaaa cgtaaagagt ttgtttttgc
     3121 atcatttccg caaactgttc actctggctt ttttctttcc cgtcgccggc gggaacggaa
     3181 ggcatcatca ttttcgcctg gacaaattgc aaaactccgg cggcaacggc aaacgccgga
     3241 aacgaagccg ccaggttgat gtgatccaaa aacatcggat ccaccgaccc ggggttggga
     3301 acaaacccgt aaagcatcgc cagcgattca tccttgaatc cgtcccagaa aacgcggtaa
     3361 agcccccaca aaaccggaat ttgcaccagc agaagcaaaa atccgcttaa cggatttatc
     3421 ttgttttccc gccaaagcgc catcatttcc agcgcctgtt tttctttgtc gtctttatac
     3481 tttttttgga tatctttcat ttggggctga atgcgtgtcg tagccttttg cacgcggatc
     3541 gccttggcgg ccagcggata aagcaatatc cggatcgcca ccgtaagcgc gatcaccgca
     3601 aaaccgaaat ctcccagcca atggtaaaaa aggatcaata cgttaaaaag aggttgatag
     3661 atcaatgcgt caaaaatatt cataagagtc ggttgaaagc cggattaaag ggattgcggc
     3721 gccaagaggc ggcttttcgc caaaattttt tccatctccg ccgccagctc ccggtaagtt
     3781 tttttctccg cgccggggcg ggcgatgacg gtgcaatcaa agccgggagt gatccgcccc
     3841 attagggcgg cggcggcggc gcggagacgg cgtttgacca aattacgcgc cggggccttt
     3901 tttgaaaccc gcttggacac cacaaaacaa aagcgggcgg cgggcagatc ggtggccgcc
     3961 gctcgaaggg acagcgccgc cccgccggcg gcgattttgc cggcaagagc ccgcttgatc
     4021 tccttttctt ttttcaggcg attctccggc ggcaacattt tgctttgaga aaattaacaa
     4081 aaatcaaacc gaaagttttt ttctgccttt ggcgcggcgc gaattcaaaa cccgccttcc
     4141 gccgacagtc ttggagcgtt cgcgaaatcc gtgagttttg cttctctttc ttttttttgg
     4201 ctgataggtt acgctcatat tcttgattgg ttattccgga attatcacgc cttcattcta
     4261 cattaaaatt ccaaaaaaac aagggcaatg gagacaaccg cttattgtct caccgcgcgc
     4321 ttgccaaaaa aacaaaaatg atgataagct gtcaatagcg tttattttga tgttttatcc
     4381 acattttacc catacttttc cacctttttt ccaccgcgaa aatcgcttgc cgttttgggt
     4441 cttttgtttt ataaaggtat gaatttcgcc ctgatctcaa aaattttccg gcaaaacctg
     4501 atccaaccgg aaaaaattgc aaaaaaaaat cccgcgatgc ggaatttgcg gcgaccccgg
     4561 caaaattaat tcccgggtta taatgaggct gtcggataat tgatcatctt ggacaatgga
     4621 aacacaaaat gcaaataacg aaaaaatctg gcgggcgatt ctggcccaga tacagttaaa
     4681 cgtttctccg gcgattttcg caacctggtt taaatcaacc agcattattt ccatagaaaa
     4741 cggagaggtt agaattgccg ttcccaattg tctggccaaa gaatggcttg aaaaaaattc
     4801 ccgcaaacaa atgatcgtcc ttctgcggga atttaacacc tcaatccggg aagttttttt
     4861 caaagttcaa caaccgccaa ccgataaaaa tatttttgtg cgggcgcgcc cggccagcgg
     4921 cgggcaattg gggcttgatg aattcaaatt caataaagaa acaaacttga atccgaaata
     4981 ccactttgat tgctttattg tggggccgca caatgaattg gcgcatgcgg ccgcggcggc
     5041 ggccgcggaa aaacccgggc aaacctataa cccgctgttt atttacggcg gcgtcggctt
     5101 gggaaaaacc catctcttgc aggcgatcgg caacaaggtc gcccacgatt tccccgaaaa
     5161 aaaaatccgt tatgtcccca cggaaaaact gaccaccgaa atcatcaacg ccatccgcgg
     5221 cggcaccatc cagaacttgc gcgccaaata tttggacacc gcggttttca ttattgacga
     5281 tattcagttt atttccggaa aagacaaaac ccaagatgaa tttttttatc ttttcaacac
     5341 tctctacaat aaaaacctgc aaatcgtctt gtcgtccgac cggccgccaa aggcgatcac
     5401 tgaactctcc aaccgcttgc gttcgcgttt tgaaggggga atgatcgccg acattagttt
     5461 gccggatttt gaaacaagaa tggcaatttt gaaagaaaaa tgccagatta tgaaaattga
     5521 tttttccgat gaaatcctgt cggcgattgc cggcaccatc caaaataatg tgcgggaact
     5581 tgaaggcgct ttaattaaaa tcgcggcgca tttcaaatta aaaaaagaac cgccgactcc
     5641 cgagatctgc cgggaaattt tggcggcaat caacccgaaa aaagtaatcg gtttcaaaaa
     5701 aatcatccag gaaaccgcca ctttttatga aattaaagaa aaagacatta tttcggactc
     5761 gcgaaaaaaa gaaattgtcg tcccgcggca agtggtgatg tatcttttaa gaaatgaaat
     5821 caacgcctca tttcctttta tcggccgaaa aatggggaac aaagaccata ccaccgtgat
     5881 gcacgcctac aaaaaaatct cttcaaaaat aaaagatgac gcatctttct
//
LOCUS       DAOQWE010000042         5886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_13836_length_5886_cov_8.139256, whole genome shotgun sequence.
ACCESSION   DAOQWE010000042 DAOQWE010000000
VERSION     DAOQWE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13836_length_5886_cov_8.139256"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1414
                     /gene="murD"
                     /locus_tag="PKK37_02250"
     CDS             50..1414
                     /gene="murD"
                     /locus_tag="PKK37_02250"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNR81231.1"
                     /translation="MKNYSGKKILILGLAREGTDSLRYFLEKYPAVQIAAADRAEIEK
                     LGPAARGLLAENPQVRFWGGNGYLEALDNYDLVVKSPGIPIHLPPVERAFKRGAITSQ
                     TEIFFKECPGMIIGITGTKGKSTTASIIYEIIKRSGKKTLLLGNIGEPMLSYLPRADK
                     NTFFVCELSAHQLYNLKQSPHIAITTNIYPEHLDYYKNYNEYILSKMNICLHQTENDY
                     FIYNGRIKEIIPWLKKTKAQKIDFSAVDWRFEEKTALTGEFNMENAKIGAIVGRLLGI
                     ADNVINAAIADFKPLPNRLELIGNFSGIDCYNDSLSTIQESAVAAIEALGPRVQTLIA
                     GGFDRNQPFDKLARSVLNSNIRNLVMLPATGIKIWREIEKQAESSGQTDRFRELSYCP
                     AADMREAVDSVFAKTKKGKICLLSAAAASFGGFADYADRGRQFKECLVEGGRLFAAKD
                     NQKK"
     gene            1419..2558
                     /gene="ftsW"
                     /locus_tag="PKK37_02255"
     CDS             1419..2558
                     /gene="ftsW"
                     /locus_tag="PKK37_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNR81232.1"
                     /translation="MAPQEQAQKPDFILLAAVAAALLLGVMILSSASATMAQVKYGDS
                     YYLIRHQLIFGVLPGLLAGVLAYKIPLSWMRKLAPPLLLISIVLLFLIFVPGFGFSAG
                     GAKRWIHLGFATIQPAEILKPVFILYLASWLASRTERKLSGKNSGQEFQNTLAAFVVV
                     VGLIGLLLLKQPDMSTFGVVALTACVMYFLAGTPIKHSLFIVFAGFLSLVVLVWLAPY
                     RADRLTSWLSPESDPLGKGYQSGQALIVVGSGGIFGQGFGASSQKYTFLPELIGDSIF
                     APYAHELGFVGAAALIVIFAVFAWRGFAVVRRSRNKFESLAAAGITFWITVQAIINIA
                     AAARMVPLGGIPLPFISYGGTAMIVELAAVGLLLNISRAAAERKL"
     gene            2795..3919
                     /locus_tag="PKK37_02260"
     CDS             2795..3919
                     /locus_tag="PKK37_02260"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNR81233.1"
                     /translation="MKILFTGGGSGGHIYPIVAVARALRKKYGDSVKLSYLGPKDNFC
                     KVVLAKEHIKVRTIMAGKMRRYANPAAIIQNVIDLLIKIPLGFLQSLFLLLFINPDAV
                     FSKGGYGSFPVALAAWILRIPIFLQESDVVAGMAVKKTVRYASVIFTSFPETEGFLNR
                     RTVPLGNPVRAQIMDGSKEEAAKALGLKGGRPLILIVGGSQGAQRINDKILDALAELL
                     QSYEIIHIAGPRNIDQVSKESKIMAKPEALEYYHPLGFAGEKVMADVYAAADLIVSRA
                     GSGSIFEIAAMKKPGILIPLPEAAQNHQVKNAYAYARTGAALVMEEGNFTRHLFLEKI
                     NDLFATPESTAAMAEAAAQFARPAAGETIADHIAKYVSQR"
     gene            3945..5513
                     /gene="gltX"
                     /locus_tag="PKK37_02265"
     CDS             3945..5513
                     /gene="gltX"
                     /locus_tag="PKK37_02265"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNR81234.1"
                     /translation="MDENNKENQNRTTPVRVRMPPSPTGFMHVGTLRMALFNYIFAKQ
                     KGGKIVLRLEDTDRQRSKKEYADDILDGLRWLGLNWDEGPFYQTQRAEIYKQYLEKLV
                     DRGKAYHCFCSSEELEAHRQYQMSRGEAPIYSGKCAAISPEEARELISCGRKSVIRFR
                     AESKKIKFNDLVRGEIEFDGSLIGDFVIAKDFENALYNFTVVVDDFEMGITHVIRGED
                     HISNTPKQILIQEALGLPRPEYAHLPIIMGADRSKLSKRHGAVGVTEYRRQGYLPEAL
                     INFTAFLGWNPGTEKEIYLMPELIRDFSFDKVQKGGAVFNSARLDYLNSCYIKNCAPG
                     DLAKLCLPYFVSAGYLSEEKSGGQTVYKNPRTGERVELEFIEKIVSYQQSRLKKLSEI
                     AEASGYFFLSELSYEKELLKWKDASFAETKLALDKIKNIFANIEAGDWKRQNLEEAVL
                     PQIAGFNLSVGKPEKDRGYLLWPLRVALCGQKMSIGPFEMADVLGKEKTAKRIDAAVE
                     MLNGSICAPNLFNR"
     gene            5513..5788
                     /locus_tag="PKK37_02270"
     CDS             5513..5788
                     /locus_tag="PKK37_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81235.1"
                     /translation="MLGIVKLLFLAALVILGIVLLPQSWVDEAKDAFAGARSYLSQTI
                     EERSPEVKENIASQAEETKKEAQIIYEGFKGEKWPQIKDWLVKKFIY"
BASE COUNT         1490 a         1213 c         1564 g         1619 t
ORIGIN      
        1 cctttttttt gttggtattc ggcttatttt tatgataaaa tattacggta tgaagaatta
       61 ttccggtaaa aaaatattga ttctggggct ggcgcgggaa gggaccgata gtttgcgcta
      121 ttttttggaa aaatatcccg ctgttcaaat tgccgccgcg gatcgggccg agattgaaaa
      181 attgggaccg gcggcccgcg gtttgctggc ggaaaatccg caggtgcgtt tttggggcgg
      241 taacggctat ttggaagcgc tggataatta cgatctggtc gttaaatctc cgggaattcc
      301 cattcatttg ccgccggtgg aaagggcctt caagaggggc gccatcactt cccaaacgga
      361 aatatttttt aaagagtgcc cgggaatgat tatcggcatc accggcacca agggaaaaag
      421 caccactgct tccattattt acgaaatcat caaaagatcg ggtaaaaaaa cactcctgct
      481 ggggaatatc ggtgaaccga tgctgtctta tttaccgcgg gcggataaaa acacgttttt
      541 tgtttgcgag ctttcggcgc atcaattata taacttaaag caaagccctc atattgccat
      601 aacaacgaat atatatccgg aacatttgga ttattataaa aactataacg aatatattct
      661 ttcaaaaatg aatatatgtt tgcatcaaac ggaaaacgat tattttatct ataatggccg
      721 gattaaggaa attatcccgt ggttgaagaa aaccaaagca caaaaaattg atttttccgc
      781 ggttgattgg cgttttgagg aaaaaaccgc gttgacggga gagtttaata tggaaaacgc
      841 gaaaatcggc gcgattgtcg gacggttatt gggaattgcc gataatgtta ttaacgcggc
      901 gattgccgat tttaaacctc tgccgaatcg cttggaactt atcggcaact tcagcggcat
      961 tgattgttat aatgactcgt tatcaacgat tcaggaaagt gcggtggcgg ccattgaggc
     1021 gctggggccg cgggtgcaga cgctgatcgc gggcggattt gaccggaatc aaccgtttga
     1081 taaattggcg cggtcggttt taaacagcaa tattcgcaat cttgttatgc ttccggccac
     1141 ggggataaaa atttggcgcg agattgaaaa acaagctgaa tcgtcagggc aaaccgatcg
     1201 ttttcgcgag ttgtcatatt gtccggcggc tgatatgcgg gaagcggttg attcggtttt
     1261 tgcaaaaaca aaaaagggga aaatatgttt actttccgcc gccgccgcga gcttcggcgg
     1321 ttttgcggat tatgccgatc gcggccggca attcaaagaa tgtctggttg agggcgggcg
     1381 tttgtttgcg gcaaaagata accaaaaaaa ataaaaaaat ggcgcctcaa gaacaagcgc
     1441 aaaaaccgga ttttattttg ttggccgccg ttgccgccgc gttgcttttg ggggtaatga
     1501 tactttccag cgcctcggcg acgatggctc aagtgaaata tggcgacagc tattatttga
     1561 tccgccacca attgatattc ggtgttttgc ccggattgtt ggcgggagtt ttggcttata
     1621 aaattccgct gtcatggatg cggaaactgg cgccgccctt attgctgatt tctattgtct
     1681 tgttgttctt aatttttgtt ccgggattcg ggttttctgc cggcggcgcg aaaagatgga
     1741 ttcatttggg ctttgccacc attcagccgg cggaaatttt aaaaccggtc tttattttat
     1801 atcttgccag ctggctggct tcccgcaccg agaggaagtt gtcggggaag aattccggac
     1861 aggaatttca aaatacgctg gcggcgtttg ttgttgtggt gggtttgatc ggacttttac
     1921 ttctaaaaca gccggatatg agcacttttg gagtcgttgc gctcacggcg tgcgtgatgt
     1981 attttttggc gggaactccg attaaacatt cgctttttat cgtttttgcc ggttttttgt
     2041 cgttggtggt cttggtttgg ctggctccct atcgggcgga tcgtttgacc tcgtggcttt
     2101 ctcccgaaag cgatcccttg ggcaagggtt atcaatccgg acaggcgctg atcgttgtcg
     2161 gttcgggcgg cattttcggt cagggattcg gcgcctcatc gcaaaaatat acttttttgc
     2221 ctgaattgat cggagattct atttttgcgc cttacgcgca tgaattggga tttgtcggcg
     2281 ccgccgctct tatcgtaatc tttgccgtct tcgcctggcg gggatttgca gtcgtccgcc
     2341 ggagtcgcaa taaatttgaa tctctggccg ccgccggtat tactttttgg attaccgttc
     2401 aggcgataat caatatcgcc gccgccgcca gaatggtgcc tttgggcggg attcccttac
     2461 cgtttatcag ttacgggggg acggcaatga ttgtggaatt ggcggcggtc ggcttgctac
     2521 tcaatatttc ccgtgccgcg gcggagagaa agctttaaaa atgtccgggt ttattgtaga
     2581 atgaaccata ttgatattcg tgaatcggtt agataattta atagacctac tgcttaaata
     2641 tttactactg caatttccat atttcggcga cgccgaattc gtcaatcctc ggcatattta
     2701 agccaatatg ccttcttcat tcctcattcg gctcgcacga aatctgaaaa tttcgtcgcg
     2761 taaatattta agcagtaggt ctaattttga atcaatgaaa atacttttta ccggcggcgg
     2821 tagcggcggg catatttatc cgattgtggc ggtggcgcgc gctttgcgta aaaaatacgg
     2881 agattccgtt aagctctcat atttggggcc gaaagataat ttttgcaaag ttgttttggc
     2941 caaagaacac attaaggtgc gcacaattat ggcggggaaa atgaggcgtt acgccaatcc
     3001 ggccgcgata atccagaatg tcattgacct tttgatcaag attcctttgg gatttttgca
     3061 gtctctgttc ttgcttcttt ttatcaatcc cgacgcggtt ttttccaaag ggggatatgg
     3121 ttcttttccg gtggcgttgg cggcctggat tttgcgaatc cccatttttt tgcaggaatc
     3181 ggacgtagtt gccgggatgg cggttaaaaa aacggttcgt tatgcttcgg tgatattcac
     3241 ttcattcccg gaaaccgaag gatttctaaa tcgcagaacg gttccgctgg gtaatcccgt
     3301 tcgcgctcag ataatggatg gctccaaaga ggaagcggca aaagcgttgg ggctgaaagg
     3361 cggccggccg ctgattttaa ttgtcggtgg atctcaaggc gcgcaaagaa tcaacgataa
     3421 aattttggat gccttggcgg agcttttgca atcatatgaa attatccata ttgcggggcc
     3481 gcgcaacatt gatcaagttt ccaaagaatc caaaatcatg gctaagcccg aggcgctgga
     3541 atattatcat ccgctgggat ttgccggcga aaaagttatg gccgatgttt atgccgccgc
     3601 ggatttgatt gtgagccgcg ccggttcggg cagtattttt gaaatcgcgg cgatgaaaaa
     3661 acccgggatt ttaattcctt tacccgaagc cgcccaaaat catcaagtga aaaacgccta
     3721 cgcttatgcc cggaccggcg ccgcgttggt gatggaggaa ggaaatttca cccgccattt
     3781 gtttttggaa aaaatcaacg acttgttcgc gacgccggaa tctacggcgg cgatggccga
     3841 agccgcggcc caattcgccc gtccggcggc gggagagaca atcgcggatc atatcgcaaa
     3901 atatgtttct caacgatgat tgacgcagtg tctttgaaaa tgatatggac gaaaacaata
     3961 aagaaaatca gaacaggaca acgccggtgc gcgtgaggat gccgccgtcg ccgacgggtt
     4021 ttatgcatgt cggaactttg cggatggccc tttttaacta tatttttgcc aagcagaaag
     4081 gcggcaagat agttttgcgt ttggaggaca ccgatcgcca gcgttcaaaa aaagaatatg
     4141 ccgatgatat tttggacgga ttgaggtggc tgggtttgaa ttgggatgag ggtccgtttt
     4201 atcaaactca aagagcggaa atttacaagc aatatctgga aaaattggtt gaccggggca
     4261 aagcgtacca ctgtttttgt tcgtccgagg aattggaagc acaccgccaa tatcaaatgt
     4321 cgcgcggcga agcgccgatc tattcgggaa aatgcgccgc aatttcgcct gaagaagccc
     4381 gggaattgat ttcttgcgga aggaaatcgg tgattcgttt ccgtgccgaa agcaagaaaa
     4441 taaaattcaa cgatttggtg cgcggggaaa tagaatttga cggatctttg attggcgatt
     4501 ttgtgatcgc caaggatttt gagaacgcac tttataattt caccgtggtg gttgacgatt
     4561 ttgaaatggg aatcactcac gtgattcgcg gcgaagatca tatttccaac actcccaagc
     4621 agattctcat ccaagaggcg ctggggttgc ctcgaccgga atacgcgcat ttgccgataa
     4681 ttatgggtgc cgatcggagc aagctttcca agcgccatgg cgcggttggc gttaccgaat
     4741 accgccggca agggtatctt cccgaagcgt tgattaattt tacggcgttt ttgggctgga
     4801 atccggggac ggaaaaggaa atttatttga tgccggaatt aatccgcgat ttttcttttg
     4861 acaaagtaca gaaaggggga gcggtgttca attcggcgcg gttggattat ttgaattcct
     4921 gttatatcaa aaactgcgct ccgggcgatc tggccaagct ctgtctgcca tattttgttt
     4981 ccgccggtta tttgagcgag gaaaaatccg gcggccaaac cgtatacaaa aatccgcgga
     5041 cgggggagag ggttgagctt gaatttattg agaagatcgt ttcctaccag caatcccgcc
     5101 tgaaaaaatt atccgagatt gccgaagcat cgggatattt ctttctctcc gaattatcct
     5161 acgaaaaaga acttctaaaa tggaaagacg cttcttttgc ggagaccaag cttgctcttg
     5221 acaaaataaa aaatatattt gcaaatatag aagcggggga ttggaagcgg caaaacctgg
     5281 aggaggccgt tttgccgcaa attgccggtt ttaatttatc cgtcggaaaa ccggaaaaag
     5341 accgcggtta tttgctatgg cctctgcgcg tggcgctttg cgggcaaaaa atgtcaatcg
     5401 gaccgtttga gatggcggat gttctgggca aggaaaaaac cgcaaaaagg attgacgccg
     5461 ccgttgaaat gttaaatggt tcaatatgtg cgccgaattt attcaatcga taatgttggg
     5521 aattgtcaaa ttgctttttt tggcggcatt ggtgattctg ggcattgttt tgttgccgca
     5581 gagctgggtg gacgaggcaa aggacgcatt tgccggcgca aggagctatc tgtcccaaac
     5641 aatcgaggaa agatcgccgg aagtcaaaga aaatatcgct tcgcaggcgg aagaaacaaa
     5701 aaaggaagcg caaatcattt acgagggatt caagggggaa aagtggccgc aaatcaagga
     5761 ctggcttgtt aaaaaattta tttattagtt tgtcccgttg cgggggaatc gtcctcggta
     5821 ttttttgaga tgagctattc gctcgtcgtc aatatattaa atgtcgcaca atcaaggtta
     5881 atggac
//
LOCUS       DAOQWE010000043         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_13941_length_5851_cov_7.788302, whole genome shotgun sequence.
ACCESSION   DAOQWE010000043 DAOQWE010000000
VERSION     DAOQWE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13941_length_5851_cov_7.788302"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKK37_02275"
     CDS             <1..705
                     /locus_tag="PKK37_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNR81236.1"
                     /translation="QKRPIDSYTFGDGKTLLVFVGAIHGGYEWNTALLAWEFIDYLKS
                     NSEIIPKNLSVAVIPALNPDGLAKIAGKEGRFAAADIPAGTNETGRFNANNVDLNRNF
                     DCNWQPKGIWRQKEVSAGSAAFSEPESAAIRDYVLKNKPAAVVFWHSQSNAVYGSMCN
                     ADMSDETRAILNAYAKASGYPAKDTFDQYQVSGDATDWLASIGIPAITVELATHQNIE
                     WQKNLAGINALFQYFK"
     gene            702..2105
                     /locus_tag="PKK37_02280"
     CDS             702..2105
                     /locus_tag="PKK37_02280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR81237.1"
                     /translation="MKITFWGATKNVTGSKHLIETQGFGLLLDCGFYQGKRTDANQQN
                     RNLPFEAKNINAVILSHAHLDHCGSLPTLVKNGFTGKIYCTPATAEIAELILLDSANI
                     QKHDCEYYNSHLAGKEPIAPIYAQEDVRRVIECFKTVDYFSSDGGWTRINKNIRFKFY
                     DAGHILGSAAVSLEIGENGDVKNLAFSGDLGRDELPILRAPENIQEAAQTLIMECTYG
                     NRTHQPMPQIFGKVKDIVKTAARKKSKIIVPAFSLGRTQELIYILHLLIKQKAIPKLP
                     IYIDTPLGDKITKIFPRHARLFDNDFKREFGDDGNAAFNFNNLKYVQSAAESKSLNGK
                     SGPFVVVAGSGMCEGGRILHHLKNGIGNADNIVLITGFQAENTLGRKILEKQTPVKIF
                     DRWYDVRAKIMVLNELSAHADREDLLNYAANIKGLNTLFLVHTETPQAEAFAQIARQS
                     LPSVTVNIPEFSQSFEL"
     gene            2281..2469
                     /locus_tag="PKK37_02285"
     CDS             2281..2469
                     /locus_tag="PKK37_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81238.1"
                     /translation="MVAFIKIICYFPIGGLKMDKVEEVAGVFLPAKDDKTNRKKTPRK
                     REEGGGDQTPHEVKQHYS"
     gene            complement(2701..3960)
                     /locus_tag="PKK37_02290"
     CDS             complement(2701..3960)
                     /locus_tag="PKK37_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNR81239.1"
                     /translation="MKAPNKLQATIKAIRTKPKTRLAVFKNLLPAQQAQICLILSKHL
                     VYDILSRLDDLEVAAILENLDPDEATDILQLFPAAKRTDLLEILSLRMKHAVERLLGF
                     DPKTAAGLMNVDYIQVGENETIAEVAKQFKIHEKRTGRLPAIIVLRSDGKLAGYLPGH
                     ELGFAQLKDKAKKYTRRIATIRHSATHEEAVDLFRAYPHAKIAVIGANDNVMGIIYSD
                     DIARVLREQTAVSLYNFAGVAKEESVFGGAGEKIKFRYKWLIINLGTSFLAAFVVSMF
                     DQTIAKYVLLAVYMPIIAGMGGNAGTQAMAVMVRGITLKQIDFKSSFGALKNELLSGF
                     ANGVINGALVAIVILFANRDPLLALVLFLAMIVNLMVAAAAGTMAPLLMARLGKDPAS
                     SATIFITTATDVLGFFAFLGLAAILLP"
     gene            4107..>5851
                     /locus_tag="PKK37_02295"
     CDS             4107..>5851
                     /locus_tag="PKK37_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR81240.1"
                     /translation="MEKNEISLKTIFGHLWEGVLPHRRLFWVCVAAFVVFQLIELAIP
                     VFYKRFFDELGQSAPAEVLIGIITVIAVINLANWLFAAIAHFSLTLVEGKIMARLKQK
                     SFDYLMLHSRKFFVNNFTGSLTQKVNRFAYAFEILMDSLVFNLIPMAVSVFGAIAVTA
                     FLAPPIAWIIAIWSLLVITANFVFSRWKIKYDVARAAADSRTTGFLSDNIVNYISVKL
                     FNGYRRESGSFKEISDQQAQKTITAWQLSNAAHIIQHLLIVVVEFAAFYYTISLWQSG
                     QAFIGAFVLVQTYVIGISHQLWGVNRMFRGIYQAMADSKEMVEILETPHQIKDTPGAK
                     ILADAKGKIDFKNIVFGFQDDRIILKDFDLSINSGEQVAIVGSSGAGKTTLTGLLLRL
                     FEPTGGRILIDGNDIREITQESLRKNISLVPQDPVLFHRSILENIRYGRPEASDEEVI
                     AAAQMAHCDKFVQKMPAGYQTMVGERGVKLSGGERQRVAIARAILKRAPILILDEATS
                     SLDSKSEALIQDALDALMKDQTVIAIAHRLSTIRKMDRVVVLKGGTIIEQGSHQSLIR
                     KRDGVYRKLWRLQSSG"
BASE COUNT         1572 a         1473 c         1387 g         1419 t
ORIGIN      
        1 caaaaacgcc cgatcgattc ctacactttt ggcgatggta aaacattatt ggtctttgtc
       61 ggggccatcc atggcggcta tgaatggaac accgcgcttt tagcttggga atttattgat
      121 tatttaaaat caaattccga aattattccc aagaatttgt ccgtggcggt gattcccgcc
      181 ctcaaccccg acgggttggc caagattgcc ggcaaagagg gacggtttgc cgccgccgac
      241 attcccgccg gcaccaacga gacgggacgc ttcaacgcca acaatgtgga tctaaaccgc
      301 aactttgatt gcaattggca acccaaaggc atctggcggc aaaaagaggt gagcgccggc
      361 agtgcggcgt tttccgaacc ggaaagcgcg gcgattcgcg attatgtttt gaaaaacaag
      421 cccgcggcgg tcgttttttg gcacagccaa tccaacgcgg tctacggctc aatgtgcaac
      481 gcggatatgt ctgatgaaac ccgcgccatc ctcaacgcct atgccaaggc gtccggatat
      541 ccggccaaag acacttttga ccaatatcag gtttcgggag acgccaccga ctggctggcg
      601 tcgatcggca ttcccgccat caccgtggaa ctggccacgc accaaaacat tgaatggcaa
      661 aaaaatctgg ccggcatcaa cgctttattc caatatttta aatgaaaatc actttttggg
      721 gcgcgacaaa aaacgttacc ggatcaaagc atttgataga aacccaagga ttcggtcttt
      781 tgcttgactg cggtttttat caaggcaaaa gaacggatgc caaccaacaa aaccgcaatc
      841 tgccgtttga ggcaaaaaac atcaatgccg tgattttgtc gcacgcgcat ttggatcatt
      901 gcggatcact gccgacattg gtgaaaaacg gttttaccgg aaaaatttat tgcacgccgg
      961 ccaccgccga aatcgccgaa ctgatcctgc tggactcggc caacattcaa aaacacgatt
     1021 gcgaatatta caattcccat cttgccggca aagagccgat cgctcccatt tacgcccaag
     1081 aggatgtgcg gcgggtgatt gaatgtttca aaaccgtgga ttatttttcc tcggacggcg
     1141 ggtggacaag aataaacaaa aatatccgtt tcaaattcta cgatgccggc catattttgg
     1201 gatcggcggc ggtatcgctg gaaatcggcg aaaatggcga cgttaaaaat ctggcgtttt
     1261 ccggcgattt ggggcgcgat gaactgccga tattgcgcgc tccggaaaac attcaagaag
     1321 cggcgcaaac cttgataatg gaatgcacct acggcaaccg cacgcatcaa ccgatgccgc
     1381 aaatattcgg aaaagtgaaa gacattgtca aaaccgcggc gcgcaagaaa agcaaaatca
     1441 tcgttcccgc gttttccctg ggaagaaccc aggaactgat ttatattctg catttgctca
     1501 tcaaacaaaa agccattccc aaactgccga tatacattga cactcctttg ggagataaaa
     1561 tcaccaaaat tttcccccgc cacgcccgtc tttttgacaa tgatttcaaa agagaattcg
     1621 gcgatgacgg aaacgccgct ttcaatttca ataatttgaa atatgtccaa tccgccgcgg
     1681 aatcaaaatc gctcaacggc aaatccggac cgtttgtcgt tgtcgccgga tcgggaatgt
     1741 gcgaaggcgg gcggattttg catcacctga aaaacggcat cggcaacgcc gataatatcg
     1801 ttttgatcac cggttttcag gccgaaaata ctttgggcag aaaaattttg gagaaacaga
     1861 cccccgtcaa gatttttgac cgctggtatg acgtgcgcgc caaaataatg gtgttaaacg
     1921 agttgagcgc ccacgccgac cgggaagatc tgctaaatta cgccgctaac attaagggat
     1981 tgaacacctt gtttttggtg cacaccgaaa cgccgcaagc cgaagccttt gcgcaaatcg
     2041 cccgacaatc ccttcccagc gttaccgtca acatccccga attttcccaa agctttgaac
     2101 tctgattttt gcaaccacgc gcccaacccg acaatgttat atttatgtca tacttatgct
     2161 atatttaaac ataacatttt cggagtaatc gctcgccggc aaaaaaattt aatttcaaaa
     2221 ttctaatact tttaaagggg tcgcacccct ttgagtacaa aggggaacga ccccaatcgg
     2281 atggttgcat ttataaaaat tatctgttat tttccaatcg gtggtttaaa aatggataaa
     2341 gtagaagagg tcgcgggcgt atttttgccc gccaaggatg ataagaccaa taggaaaaaa
     2401 acccctcgca agcgcgaaga gggtggcggg gaccaaaccc cgcacgaggt gaaacaacat
     2461 tatagttgag tttcacattt tttttacaca aactaaaaat tttaaaaacc ggtttgatta
     2521 aatctccgat attttaaagg gtctgttgcc gaattggctt tgaagcgaaa ttttcagatt
     2581 tcgcgcgagc caaatgagga aagaggaggc atacttggct taaagtatgc cgacgattga
     2641 cgaatttggc gcagccgaaa gatggaaatt gcagccaaag ccaattcggc aacagaccct
     2701 ttacggcagc aagatggcgg ccagacccag gaaggcgaaa aatcccaaaa catcggtggc
     2761 ggtggtgatg aaaattgttg ccgacgacgc cgggtccttg cccaatctgg ccatcaacaa
     2821 gggagccatg gtccccgccg ccgcggccac catcaagtta acgatcattg ccaaaaacaa
     2881 aaccagcgcc aacaacggat cgcggttggc aaacaggatg actatcgcca ccagtgcgcc
     2941 gttaataacg ccgttggcaa aaccggaaag caattcgttt ttcagcgcgc cgaaagaaga
     3001 cttaaaatca atctgtttga gcgtgatgcc gcgcaccatc accgccatcg cctgcgtgcc
     3061 ggcattgccg cccatccccg cgatgatcgg catatacacc gccagcaaaa catatttggc
     3121 gatggtttga tcaaacatac tcaccacaaa cgccgccaaa aacgaggtgc ccaagttaat
     3181 gatcagccat ttatagcgaa attttatttt ctcccccgcg ccgccaaaca cgctttcttc
     3241 cttggcaaca ccggcaaaat tatacaatga caccgccgtt tgttcgcgca ggacgcgggc
     3301 gatatcgtcg gaatagatga tgcccatcac gttatcatta gctccgatca cggcgatttt
     3361 ggcatgagga taggcgcgaa agaggtccac cgcttcctcg tgggtggccg aatggcggat
     3421 ggtggcgatt ctgcgggtat attttttggc cttgtccttt aactgtgcaa atcccaactc
     3481 gtggccgggc aaatatccgg ccagcttgcc gtcggatctc aaaacgatga tcgccggcaa
     3541 tcggccggtg cgcttttcgt ggattttgaa ttgcttggcc acttcggcaa tggtttcgtt
     3601 ttcgcccact tggatgtaat ccacattcat caaccccgcc gccgttttgg gatcaaaccc
     3661 caaaagcctt tccaccgcgt gtttcatccg caaactcaaa atttccaaaa gatcggttct
     3721 tttcgccgcc ggaaaaagct ggagaatgtc ggtcgcttcg tcgggatcca gattttccaa
     3781 aatcgcggcg acttccaggt cgtccaaacg gctcaaaata tcatagacca aatgcttgct
     3841 caggatcagg caaatttgcg cctgttgagc ggggagcaga ttcttaaaaa ccgccaggcg
     3901 cgtttttggc ttggtgcgaa tcgctttgat tgtcgcttgc aatttgttgg gagccttcat
     3961 tattccatca atcaaaaaac atctgacttt ttaattatag ccgtaaaaaa atttttcacc
     4021 aaaacaaaac cggccggcac aaaaaattgc ggattttccc tttaaaggtt aggatataat
     4081 tggcgggaaa attgaaaaac gaaaaaatgg aaaaaaatga aatttcgctg aaaacgattt
     4141 ttggccatct ttgggaaggc gttctgccgc accggcggct tttttgggtt tgcgtcgccg
     4201 cgtttgttgt ttttcagttg attgaactgg caattccggt tttttacaag cggttttttg
     4261 acgaattggg ccaatcggcg ccggcggaag ttttaatcgg catcatcacc gtcatcgcag
     4321 tcatcaatct ggccaattgg ctgttcgcgg cgattgccca cttttctctg accttggtgg
     4381 aaggcaaaat aatggcgcga ttgaaacaaa aatcgtttga ctatctgatg cttcattcgc
     4441 ggaaattttt cgtcaacaat ttcaccggaa gcctcaccca aaaggtgaat cgtttcgctt
     4501 acgcgtttga aatattgatg gacagtttgg tttttaatct gatccccatg gcggtgagcg
     4561 ttttcggggc gatcgcggtc accgcgtttc tggcgccgcc catcgcatgg attattgcaa
     4621 tttggtcgtt gctggtaatc accgccaatt tcgttttctc gcgctggaaa atcaaatatg
     4681 atgtggcgcg ggcggcggca gattcaagaa ccaccggatt tctttccgac aatatcgtta
     4741 attatatttc agtcaaactc tttaacggtt atcggcgtga gtccggcagt ttcaaagaaa
     4801 tttccgacca gcaggcgcaa aaaacgatta ccgcatggca actttcaaat gccgctcata
     4861 tcatccagca tctgttgatt gtcgtggtgg aattcgccgc gttctactac accatttctc
     4921 tctggcaatc cggacaggca tttatcggcg ctttcgtttt ggtgcaaact tatgttatcg
     4981 gcatttccca ccaactctgg ggagtaaatc ggatgtttcg cggaatctat caagccatgg
     5041 ccgattccaa agaaatggtg gaaatcctgg aaactcccca tcaaatcaaa gacactcccg
     5101 gggcaaaaat tttggcggac gccaagggaa aaattgattt taaaaacatt gttttcggat
     5161 ttcaagacga tcggataatt ttaaaagatt ttgatctgtc catcaattca ggcgagcaag
     5221 tggccattgt ggggtcgtcg ggcgcgggaa agaccactct caccggcctg ttattgcgtc
     5281 tttttgagcc gaccggcggc cggatattga ttgacggcaa cgatatccgc gaaatcactc
     5341 aggaaagttt aagaaaaaac atttccctgg tgcctcagga tccggttttg ttccatcgct
     5401 caatcttgga aaatatccgc tacggccggc cggaagcgtc cgacgaagaa gtaatcgccg
     5461 ccgcgcaaat ggcgcattgc gacaaattcg tccaaaaaat gcccgccggc taccaaacaa
     5521 tggtgggcga gcggggcgtc aaactttccg gcggcgaacg ccaacgagtg gccatcgccc
     5581 gggcgattct gaagcgtgcg ccgattttga ttttggacga ggcgacctca tctttggact
     5641 caaagtccga agcccttatt caagacgcgc ttgacgcctt aatgaaagac caaactgtca
     5701 tcgccatcgc acaccgcctt tccaccatcc gcaaaatgga ccgcgtggtg gtgctaaaag
     5761 gcggcacaat cattgaacaa ggctcccatc agtctttgat ccgcaaaagg gacggcgtct
     5821 accgcaaact ctggcgcttg caatcttccg g
//
LOCUS       DAOQWE010000044         5827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_14035_length_5827_cov_7.275468, whole genome shotgun sequence.
ACCESSION   DAOQWE010000044 DAOQWE010000000
VERSION     DAOQWE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14035_length_5827_cov_7.275468"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2457
                     /locus_tag="PKK37_02300"
     CDS             <1..2457
                     /locus_tag="PKK37_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81241.1"
                     /translation="AAAACLFLYLFSAADVALATATPKYVVVHAWNPGPDNPNYFKGC
                     STQWIGPMLDAIGKRGNGAVQLGISDVIYALNTDDAEIRSCLDNFFSSSEQHNIPFML
                     HIDTERFWDSRPDLWNWFDPAASGYNPANIDNVEWSGWSEGTKESFINWGYPIKLPPK
                     PCYESPAVKRVVENKFSIISDKINEWRKKLADEGREYLFLGVNLGWETGIDDYRDAGW
                     AAAPYKYRLGYNALSKNGFGAANPPANINSELVKVVADFNKFRAKLLYDRGIPKDKIF
                     THSWAADDKVPLGSDGYTQNGPISSAKNDYGVLGVSLYPPAYDTNRVKNEIGTAPWAL
                     IESPPSAILTAPRGYLSVKDWLNFGNCKLAVVYGLSASQSDTLPQIRQLATLLPDNGN
                     PIGFFDHADCNYVGGWVCDKDDFGRSLKVKIFADGVIGQGSLIGTTFANVNRENAVAG
                     LCGGDSKHGFNFPMPASLKDGKSHSIYVYGEDFPSGNNVLLGVSNSSANSIVVTCAAA
                     QFCTPGSANGCKVCNSGGSAWVDTDSKCQSGQICADGVCQNVCISKTCTGMGYSCGSA
                     GDGCGGTLDCGACPSGYACSGNQCVSMCVPKTCARMGYFCGNQSDGCGGILNCGTCAP
                     GKVCSQGGCVSNCASHAYKKCSDGNLYWYNSCNNKEELAHNCEVLEISDYRCNGKMLQ
                     KKIVIGSCANDICIANSAWTDAADCSATGKICENGACVVSKIVPVDRICIPQKISGCK
                     VCNTAGTDWINDNLRCAGGETCVDGACVSAAGGTIKPKDLAPATRAEIMQKIAEIKQL
                     MIQLITQLIAELQRQLALEQ"
     gene            2511..3479
                     /locus_tag="PKK37_02305"
     CDS             2511..3479
                     /locus_tag="PKK37_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNR81242.1"
                     /translation="MEIIEKLKQANLRGRGGAGFPAWQKWQMVKDAPGETKYVVCNVS
                     EGEPAVAKDGHILEKWPDIVIEGIKLAMEEVGAKKGYLYLRHDYFDKFKNALAEIIGA
                     RVIEIIREPGGYLGGEETALLDSIEGKPCEPRIKPPFPPQTGLWGCPTLINNLETLYF
                     AARIARGEYKNTRFYTIAGDCACPGVFERSESLTIKEILEKTGNLPQFKFFVQAGGGA
                     SGTIMLLDELEVPLSGAGSIVIYNKAKTDPRQLGLKWINFFIAENCGKCVPCREGVYR
                     LREIFSGEPLDIARAQSIVKLLAAASFCPFGKGVANSIGSLLEKLL"
     gene            3476..4003
                     /locus_tag="PKK37_02310"
     CDS             3476..4003
                     /locus_tag="PKK37_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR81243.1"
                     /translation="MKSKENNYAFVDSQNLNLSVQSLGWRLDFAKFRVYLKEKYGVSK
                     AFLFIGYVEGNNDLYAFLQNAGFVCIFKPTLKHNDGSTKGNCDAELVLWSMIEYRNYN
                     KAVIVTGDGDFYCLIKYLIENDKLLALMVPNKFQFSALLKFKICRSFLRFMNDLKKKL
                     AYIPKKKKPHKDETS"
     gene            4072..5820
                     /locus_tag="PKK37_02315"
     CDS             4072..5820
                     /locus_tag="PKK37_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNR81244.1"
                     /translation="MKIIIDNREVEAREGQTILEAARENGIEIPALCYHPDLCVKANC
                     RMCVVEIEGVAAPQAACATKIKEGTKVVTQSENLTALRKSNLELLLGQHRRDCAKCIW
                     QGKCALLKYALELKVDLNAYPLRNPEPEIIKFGPIILDRSKCINCRNCVDMCSRQTGS
                     KRGDEAECKSDGFLELEGRGYESRISPSADEKKDCIYCAQCVVHCPVGALDVESAVEA
                     VGALLKDKKGKTLVVQFAPSIRASIGEMFGIPHGRVVTGQLVASLKKLGFDKVFDTNM
                     SADLTTVEEAKELVQRVKENKPLPMFTSCCPAWIRYVEFYRPDLIPHITTVKSPQNML
                     GSLIKTRWAENNNINPEDIVSVSAMPCAAKKYDAFKPEEKINDLSPADFVLTTRELGR
                     LLQKNRIDLKAMPEEIADDPFGVYTGAGTIFGATGGVTEAAARTAHWLITGKNMEKID
                     LEAVRGIEGLKKAEIEIGGVKLKIAIANGMENAKMVLDDLAKDPKAYDYVEVMACPGG
                     CIGGGGQPLPVNDEIRRKRAQGLYSDDAAKTIRLAHESPAVKAVYDDLQNDEHKIHEI
                     LHTHYAQKGKSEIKNN"
BASE COUNT         1530 a         1226 c         1649 g         1422 t
ORIGIN      
        1 gcggcggccg cttgtctgtt tttgtatctc ttttcggcgg cggatgttgc gcttgcgacc
       61 gcgactccca agtatgttgt tgtccacgcg tggaatcccg gacccgacaa tccgaattat
      121 tttaaaggat gttcaacgca gtggattggg ccgatgctgg atgcgattgg aaaaaggggg
      181 aacggagcgg ttcagctggg aatttcggat gtgatatatg ctttgaatac cgatgacgcg
      241 gaaatccggt cttgcttgga taattttttc agttcttctg aacaacacaa catccctttt
      301 atgttgcata ttgacacgga gcgtttttgg gattcccggc cggatttgtg gaactggttt
      361 gacccggcgg cgtcgggcta caatccggca aacattgata atgttgaatg gagcggctgg
      421 agcgagggaa ccaaagagtc gttcatcaac tggggttatc cgataaaatt gccccccaag
      481 ccttgctatg aaagtccggc ggtcaagcgg gtcgttgaga ataaatttag cattatctcc
      541 gataaaataa acgaatggcg caagaaactg gcagacgaag gaagggagta tctgtttttg
      601 ggggttaact tgggatggga aaccgggatt gacgattatc gcgatgccgg ctgggcggcc
      661 gcgccttata aataccggtt gggttataac gcactttcaa aaaatggatt tggcgccgcc
      721 aatcctcccg ccaacataaa ttcggagctg gtaaaagttg tcgccgattt caataaattt
      781 cgggccaagc ttttgtatga ccgcggcatt cccaaagata agatattcac ccattcctgg
      841 gcggcagacg acaaagttcc ccttggttca gatggttata cccaaaatgg cccgatttca
      901 agcgcgaaaa acgattacgg tgttttgggg gtgagcttgt atccgcccgc gtatgacaca
      961 aatcgggtaa aaaacgagat tggaacggcg ccttgggccc ttattgaatc tcccccgagc
     1021 gcgatattga ccgcgccgcg gggctattta agcgtcaagg attggcttaa tttcggtaat
     1081 tgtaaattgg cggtagttta cggattgagc gcgtctcaat ccgatacctt gcctcaaata
     1141 cgccagctgg caacactact gccggataat ggcaatccaa tcggcttttt tgaccacgcc
     1201 gattgcaact atgtcggcgg ctgggtttgc gataaagacg atttcggtcg ttctcttaaa
     1261 gttaaaattt ttgccgatgg cgttatcggg caaggatcat taattggcac gacatttgcc
     1321 aatgtgaatc gtgaaaacgc ggtggccgga ttatgcgggg gcgacagcaa gcacggattc
     1381 aattttccga tgcccgcttc tctcaaagac ggcaaaagcc acagtattta tgtctacggg
     1441 gaagactttc cgtcggggaa taatgtgttg ctcggggtat ccaattcaag cgcgaactca
     1501 atcgtagtaa cttgcgccgc cgcccaattt tgcacgccgg gttcggcgaa cggttgcaag
     1561 gtttgcaatt ccggcggcag tgcttgggtg gataccgatt ccaaatgcca aagcgggcag
     1621 atttgtgccg atggcgtttg tcaaaatgtt tgcatttcca aaacttgcac cggcatgggt
     1681 tattcctgtg ggtcagctgg tgacggttgc ggcggtacgc tggactgcgg cgcctgtccg
     1741 agcggatatg cgtgctccgg caaccaatgc gtttcaatgt gtgttcccaa aacttgcgcc
     1801 cgcatgggtt atttttgcgg gaaccaaagc gatggttgcg gggggatatt aaattgcggc
     1861 acttgcgcgc cgggcaaagt ttgttcccaa gggggatgcg tttccaattg cgcctcccat
     1921 gcttataaaa aatgttcgga cggcaatctg tattggtata attcctgcaa caacaaagag
     1981 gaattggcgc ataattgcga ggtactggaa atttccgatt atcgttgtaa cggtaaaatg
     2041 ctccaaaaga agattgtcat cggtagttgc gccaatgata tttgcatcgc caattcggcc
     2101 tggaccgatg ccgcggattg ttccgccacc ggcaagattt gtgaaaacgg cgcgtgcgtg
     2161 gtttcaaaaa ttgtgccggt ggatcggata tgcatacctc aaaagatttc cggttgtaag
     2221 gtttgcaata cggcgggtac ggactggatc aacgacaatt tgcgatgcgc tggcggggaa
     2281 acgtgcgttg acggcgcttg cgtttccgcg gccggcggca caattaagcc aaaagacttg
     2341 gcgccggcga cacgcgccga aattatgcaa aaaattgccg aaatcaagca attaatgata
     2401 caattaatca cccagcttat cgccgaactg caaaggcaat tggcgctgga gcaataaatc
     2461 gcgccgggac atagccccgg ggtattctgg cgggtaaata aaaccaaaat atggaaatca
     2521 ttgaaaaatt gaagcaagcg aatttgaggg ggcgtggcgg cgcggggttt cccgcatggc
     2581 aaaaatggca aatggttaag gatgcgccgg gcgaaaccaa atatgttgtt tgcaatgtgt
     2641 ctgaaggcga gccggcggtg gccaaagatg gccacattct ggaaaaatgg cccgatatcg
     2701 tgattgaagg aatcaaactg gccatggagg aggtgggggc aaaaaaaggc tatttatatt
     2761 tgcgtcatga ttattttgat aagtttaaaa atgcgctggc cgaaattatt ggcgcacggg
     2821 tgatagaaat catccgcgaa ccgggcgggt atctgggcgg agaagaaacc gcgctgttgg
     2881 attccattga aggcaagccg tgcgaaccgc ggattaagcc gccattcccg ccgcaaacgg
     2941 gtctttgggg ctgtcccact ttgatcaata atttggagac attatatttt gcggcaagga
     3001 ttgcccgcgg tgaatataaa aatacgcgct tttacaccat tgccggcgat tgcgcttgcc
     3061 cgggagtgtt tgagcggagc gaaagcttga caatcaagga aattttggaa aagaccggca
     3121 atctgccgca attcaaattt tttgtgcagg ccggcggcgg tgccagcggc accattatgc
     3181 tgttggatga attggaggtt cctttgtccg gcgcgggatc aatcgtgatc tataacaaag
     3241 ccaaaaccga tccgcggcaa ctggggttga aatggataaa ttttttcatc gccgaaaatt
     3301 gcggcaagtg cgttccctgc cgcgaaggcg tttatcgcct gcgcgaaatc ttttcaggag
     3361 agccgcttga catcgcgcgg gcgcaaagca tcgtcaagct gctggcggcg gccagcttct
     3421 gccccttcgg caaaggcgtc gccaattcca tcggcagttt gttggagaaa ttgttatgaa
     3481 atccaaagaa aataattatg cgtttgttga cagccagaat ttgaatttga gcgtgcaatc
     3541 gctggggtgg cgtttggatt ttgcgaagtt cagagtttat ttgaaagaaa aatatggtgt
     3601 ttcaaaggcg tttttgttta ttggatatgt tgagggaaat aacgatttat acgctttttt
     3661 gcaaaatgcc ggatttgttt gtatttttaa accgacatta aagcacaacg acggttcaac
     3721 aaaagggaat tgcgatgcgg agttggtatt atggtcaatg attgaatatc gaaattacaa
     3781 taaagcggtt attgtgaccg gcgatggtga tttttattgt ttgattaagt atttgataga
     3841 aaatgataaa cttttagcgt taatggtgcc gaataaattt caattttcag cattattgaa
     3901 atttaaaatt tgccggagtt ttttaagatt tatgaatgat ttaaaaaaga aactggcata
     3961 tattccgaaa aagaaaaagc cccataagga cgaaacctca tagggttgtt ttttccgttc
     4021 gtgattgtat tttaagttta tattgttgat ttattttgtc aagtaaaaaa tatgaaaata
     4081 atcattgata atcgggaggt ggaggcaagg gaggggcaga caatattgga ggcggcgcgg
     4141 gagaacggga ttgaaattcc ggcgctgtgt tatcatccgg atctgtgcgt gaaggcaaat
     4201 tgccggatgt gcgtggtaga gattgaggga gtggcggctc cacaggcggc ctgtgcgaca
     4261 aagataaaag agggaacgaa agtggtcacg caaagcgaaa atttgacagc gttgcgcaaa
     4321 agcaatttgg aattgctttt gggccagcat cggcgcgatt gcgccaaatg tatttggcag
     4381 ggcaagtgcg cgctgttgaa atacgcgctg gaactgaaag ttgatttgaa tgcctatccg
     4441 ctgcgcaatc ccgaacccga aattatcaaa ttcgggccga taattttgga ccggtcaaag
     4501 tgcatcaatt gccgcaattg tgtggatatg tgttcgcgcc agaccggttc caagcgcggg
     4561 gacgaggccg agtgcaaatc tgatggcttt ttggaattgg aagggcgggg atatgaaagc
     4621 cggatttcgc cttccgcgga tgagaaaaaa gattgcattt attgcgccca gtgcgtggtg
     4681 cactgcccgg tgggcgcgct ggatgtggaa agcgcggtgg aggcagtggg cgcgctactg
     4741 aaagataaaa aagggaaaac gctggtggtg caattcgcgc cgtcaatccg ggcgtcaatc
     4801 ggggaaatgt ttggcattcc ccacggccga gtggtgaccg gacaattggt ggcatccttg
     4861 aaaaaattgg gatttgacaa ggtgtttgac acgaatatgt cggcggattt gaccaccgtg
     4921 gaagaggcca aagagttggt gcaaagagtc aaggaaaaca aacctttgcc gatgttcact
     4981 tcctgctgtc cggcgtggat ccgctatgtg gaattttatc ggccggactt gatacctcac
     5041 attaccacgg tcaaatcgcc gcaaaatatg ctgggatcgc tgatcaaaac ccgctgggcg
     5101 gaaaacaaca atatcaatcc cgaagatatt gtttcggtgt cggcgatgcc ttgcgcggcg
     5161 aaaaaatacg acgcgttcaa gcccgaagaa aaaatcaacg atctgtcgcc ggcggatttt
     5221 gtgttgacca cccgcgaact gggacgctta ctgcagaaga atcggattga tttgaaagct
     5281 atgcccgaag aaattgccga tgatccgttc ggcgtttata ccggcgccgg cacgattttt
     5341 ggcgccaccg gcggagtgac cgaggcggcg gcacgcacgg cgcactggct gatcaccggc
     5401 aagaatatgg agaagattga tctggaagcg gtgcgcggca ttgaggggtt gaaaaaagcc
     5461 gaaattgaga tcgggggcgt taaattgaag attgcgattg ccaacggaat ggaaaatgct
     5521 aaaatggttt tggacgattt ggccaaagat cccaaagcct acgattacgt ggaggtgatg
     5581 gcgtgcccgg gaggatgcat cggcggcggc ggccaacccc tgccggtcaa tgatgaaatc
     5641 cgcagaaagc gcgcgcaagg gctgtattcc gacgatgcgg ccaaaactat tcgccttgcc
     5701 cacgaaagcc cggcggtcaa agcagtctat gacgatctgc aaaacgatga acacaaaatc
     5761 cacgaaatcc tgcacacgca ttacgcgcaa aaaggcaaaa gcgaaatcaa aaataattaa
     5821 aaaataa
//
LOCUS       DAOQWE010000045         5713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_14367_length_5713_cov_7.269353, whole genome shotgun sequence.
ACCESSION   DAOQWE010000045 DAOQWE010000000
VERSION     DAOQWE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14367_length_5713_cov_7.269353"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..420
                     /locus_tag="PKK37_02320"
     CDS             118..420
                     /locus_tag="PKK37_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNR81245.1"
                     /translation="MAKSKNTGGTKLGRDPRPKYLGVKLYAGQAAKIGSVIVRQRGSE
                     ILPGKNVKMGKDDTLYAIAEGTVAYREIRKKSFSGTQRKAKVAEIIETKTVGKAVK"
     gene            complement(614..2245)
                     /locus_tag="PKK37_02325"
     CDS             complement(614..2245)
                     /locus_tag="PKK37_02325"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNR81246.1"
                     /translation="MSKYIFVTGGVMSSIGKGITAASIGKILKSKGFSVTAVKFDPYI
                     NVDAGTMNPIEHGEVFVTDDGTECDQDVGNYERFLDENLSANNYATTGQVYLSVINRE
                     RNLEYNGKCVEVVPDIPNEVIRRIKAAAKTAAADFTIVEIGGTVGEYQNMLFLEAARM
                     MKLGNPGSVLFVLVGYLPIPATVGEMKTKPVQSACKLLNGAGIQPDFIIGRASQPIDA
                     PRKQKISIFCNVDPKDVISAPDAASVYEVPLNFANDHLGERILLKLKIRPKKRNLKDW
                     EDFVSSIHSSANPVKIGIVGKYFESGKFTLIDSYISVIEAVKHVAWFFKKKPEIVWLS
                     AENYEKDTKNLKELDEYDGIIVPGGFGNRGIEGKIMAIEYCRKNKIPFLGLCLGMQLA
                     TIEFARNVCGVKSATSAEFDPKAAEPVIDLMPEQKALIKNKKMGATMRLGAYDCELTP
                     GTRAYDAYNRKKMISERHRHRYELNNDYRQALERGGLVISGNNPQKKLAEIVELPDHP
                     FFMAAQFHPEFKSRPQRPHPLFREFVRAAIANSKS"
     assembly_gap    2260..2359
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2360..2754)
                     /gene="rplI"
                     /locus_tag="PKK37_02330"
     CDS             complement(<2360..2754)
                     /gene="rplI"
                     /locus_tag="PKK37_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNR81247.1"
                     /translation="MKVILLQDIEKLGKKFEVKEVADGFAKNHLLPKKMVQPATKNAL
                     IWAQTQREIAAKNEEKELIEIQKKVSALDGREFVMEVKTGEKDQLFESVNSSKIAELL
                     KENGIDADKKQIDLKEPIKELGDWRVKIN"
     gene            complement(2908..4191)
                     /locus_tag="PKK37_02335"
     CDS             complement(2908..4191)
                     /locus_tag="PKK37_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR81248.1"
                     /translation="MKKFRKDFCSHEPAIVVGVVKIKCDFNMGMLNNENKKKEGRDFW
                     GFLIAVTALASFAIGNWTATSSCPKLPPADADMSLFWQTWHALEDNFVDKDQIDHQKM
                     IYGAISGMVAALEDPYTSFFNPEDSKKFLEDTSGAFEGVGMEIGIKENRLCVIAPLEN
                     SPAKAADIRAGDFITQIDGESTAGITTDEAVDLIRGPKGSQVKLTLFREEWKETRDFE
                     ITRDVIKVPSLDWELKDGDIAHIKLYQFTEKASGDFKAAAIDIINSPAKAIVLDLRNN
                     PGGYLAVAQDIAGWFIKKGNVITRESFGEGIPETEHKSAGPALLEKYPVVVIINGGSA
                     SASEILAGALRDNRQVKIVGAQSFGKGSVQELVPLAGGASLKVTIAKWLTPNGDQISE
                     KGLTPDAEIKISAEDEENEKDPQLEKAIEIIKDIK"
     gene            4330..4668
                     /locus_tag="PKK37_02340"
     CDS             4330..4668
                     /locus_tag="PKK37_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81249.1"
                     /translation="MTKTTIEYGDFEKLDLRVGKILAANEPEWSNKLIEMKVDFGSEI
                     GERTILAGLRKFVNFADLIGNKYVFIINLPERKLGEGVSQGMMLAAGGDSTHLPLRVD
                     DAIAEGSQIR"
     gene            complement(4929..5189)
                     /locus_tag="PKK37_02345"
     CDS             complement(4929..5189)
                     /locus_tag="PKK37_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR81250.1"
                     /translation="MRNKTIQFNTQVWKEGDMYVAYAPQLDVSSCGKTVDEAKKNIKE
                     AVGLFISEAQKMDTLNQILDEAGFSGQQAPEIIAFEKMSLAV"
     gene            complement(5238..5498)
                     /locus_tag="PKK37_02350"
     CDS             complement(5238..5498)
                     /locus_tag="PKK37_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81251.1"
                     /translation="MAEKKKTSAGAQTPPNQKSLSENLKELAEISAWFSGQEDLDVEA
                     GLEKVKTAAALIKSSKDKLDKLENEFKEIEKDFSEITNGNDQ"
     gene            complement(5476..>5713)
                     /locus_tag="PKK37_02355"
     CDS             complement(5476..>5713)
                     /locus_tag="PKK37_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81252.1"
                     /translation="KIKNRLAGAAEKIAHFQELIAARDPARQLRLGYCIAKINGTIIK
                     RAGDAKAGDDMDIVLSDGIINSQIKKVYGREKEN"
BASE COUNT         1342 a         1305 c         1250 g         1716 t
ORIGIN      
        1 tttttgacaa aaggcaagat ttgcgctatt atttgcgtgt aaatcttata actttcttat
       61 tgtttgttgg ctccggattc tttgcgaatc ggtgatcaat gataatttat aaaaaaaatg
      121 gcaaagtcaa aaaacaccgg cgggacaaaa ttgggccgcg atccgcggcc gaaatatctg
      181 ggcgtgaagc tctatgcggg gcaggcggcc aaaattggaa gcgtgatcgt gcgccagcgc
      241 ggttcggaaa tcttgccggg caaaaatgtg aaaatgggca aggatgacac tctctatgcg
      301 atcgccgagg gcaccgttgc ttaccgcgaa atccgcaaaa aatcgttttc cggcactcaa
      361 agaaaggcca aagtagctga aattattgaa acaaaaacgg tgggcaaagc cgtaaagtag
      421 aatacaattt ttatcttttt ggaattaaag acatccccgg cgaagggatg tttttgtttc
      481 aataattaaa tctcaagcac caagcactaa attctaaata aatccaaaat ccaaatattc
      541 aattttcaaa caaaattcaa aattcgtttt gttttttaaa ttttgatatt tgatattgtt
      601 ttgggtttag agattaggat ttagagtttg cgattgcggc gcggacgaat tcgcggaaca
      661 gcgggtgggg tctttgcggg cgggatttga attccgggtg gaactgcgcc gccataaaaa
      721 acggatggtc cggcaattca acgatttcgg ccaatttttt ttgcggattg ttgcccgaga
      781 tcactaatcc ccctctttcc aacgcttggc gataatcatt gttcaactcg tagcggtggc
      841 ggtggcgctc ggaaatcatt tttttccggt tataggcgtc ataagcgcgg gtgcccggcg
      901 ttaactcgca atcataggcg cccaggcgca tggtggcgcc catttttttg tttttgatca
      961 gcgctttttg ttcgggcatc agatcaatca ctggttcggc ggctttggga tcaaattccg
     1021 cggaggtggc tgattttacc ccgcacacat tgcgggcgaa ttcaatggtt gccaactgca
     1081 ttcccaggca caatcccaaa aacggaattt tatttttacg gcaatattca attgccataa
     1141 ttttgccttc aatccctcga ttgccgaatc ctcccggaac gatgattccg tcatattcgt
     1201 ccagttcttt taaatttttg gtgtcttttt cgtaattttc ggcggacagc caaacgattt
     1261 ccggcttttt tttgaaaaac cacgctacgt gcttgacggc ttcaatcacg gaaatgtagg
     1321 aatcaatcag cgtgaatttt cccgattcaa aatatttgcc gacgatgcca atttttaccg
     1381 gattggcgga cgagtggata ctgctcacaa aatcttccca gtctttcaga ttcctttttt
     1441 tgggtcttat tttcaatttc agcagaatcc tttctcccag atgatcgttg gcgaaattta
     1501 acggcacctc ataaacgctg gcggcgtcgg gagcagaaat gacatctttg ggatcaacgt
     1561 tgcagaaaat ggaaattttt tgtttgcgcg gcgcgtcaat gggctggctg gcgcggccga
     1621 tgatgaaatc cggctggatg cccgcgccgt ttaatagttt gcaggcgctt tgcaccggct
     1681 tggtcttcat ttcccccacc gtcgccggaa tgggcagata gccgaccaaa acgaacaaca
     1741 cgcttccggg atttccaagc ttcatcatcc gggcggcttc caaaaacagc atattctggt
     1801 attcgcccac ggtgccgccg atctccacaa tggtgaaatc tgccgccgcg gtttttgccg
     1861 cggctttgat gcgccggatg acttcattgg gaatgtcggg taccacttcc acgcatttgc
     1921 cgttatattc cagatttcgt tcgcgattga tcaccgacaa atagacctgt ccggtggtgg
     1981 catagttgtt tgcgctcaaa ttctcgtcca aaaatctttc ataattgccc acatcctggt
     2041 cgcattcggt gccgtcgtcg gtgacaaaaa cctctccgtg ctcaatcgga ttcattgtgc
     2101 cggcgtcaac attgatatag ggatcaaatt tcaccgcggt gaccgaaaat cccttgcttt
     2161 ttaaaatttt accgatggag gcggcggtga ttcccttgcc gatggaagac attacccctc
     2221 cggtgacgaa aatatatttg ctcataaaaa attgaaaatn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnna aattgatttt cacccgccag tcgcccagtt cctttatcgg
     2401 ttctttaagg tcaatctgct tcttatcggc atcaatccca ttctccttga gaagttcggc
     2461 gatttttgag gaattgaccg attcaaacaa ctgatccttt tcgccggttt tgacttccat
     2521 aacaaattcc cggccatcaa gcgcgctcac ctttttttga atctcaatta actctttttc
     2581 ttcgttcttg gcggcgattt cgcgctgggt ttgcgcccaa attaaagcgt ttttcgtggc
     2641 gggttgcacc atctttttcg gcaaaagatg gtttttggcg aatccgtcgg caacttcttt
     2701 cacttcaaat ttctttccca atttctcaat gtcttgcaag agtatgacct tcatagtaag
     2761 tttaaaattt aaaaatcaaa aattaaaatg acaatgtaaa attaaaaatt ttttaattcc
     2821 ggattaatga tcaattattg gcggtaatga ttatttaaca tttccagctt gtttacttgg
     2881 gatttttcaa ctttcaattc ttgtattcta ctttatatcc ttaataattt caatggcctt
     2941 ttccagttga gggtcttttt cgttttcttc gtcttcggcg ctgatcttga tttcggcatc
     3001 cggcgtcaat cctttttcgc tgatttgatc tccattgggc gtgagccatt tggcgatggt
     3061 cactttcaaa gacgcgcctc ccgccagggg caccagttcc tgcaccgatc ctttgccgaa
     3121 agattgtgcg ccgacgattt tgacttggcg gttgtcgcgc agggcgccgg caagaatttc
     3181 cgaggcggat gccgaacctc cattaataat taccacgacg ggatattttt ccaacagtgc
     3241 cggcccggcg gatttgtgtt cggtttcagg aatcccttcg ccgaagcttt cgcgagtgat
     3301 gacatttcct ttcttaatga accatccggc gatatcttgt gccaccgcca gataaccgcc
     3361 cggattgttg cgcaggtcca aaacgattgc ttttgccgga ctgttgatga tgtcaattgc
     3421 cgcggccttg aaatcgccgc ttgctttttc ggtgaattga taaagcttta tatgagcgat
     3481 gtcgccatct tttaactccc agtccagaga gggtaccttg atcacgtccc gcgtgatctc
     3541 aaaatcgcgg gtttctttcc actcttcccg gaatagcgtg agtttcacct ggcttccttt
     3601 ggggccgcgg ataagatcca ccgcttcgtc ggtggtgata ccggcggtgg attcgccgtc
     3661 aatttgggtg atgaaatctc cggcgcggat atcggcggct tttgccggcg aattttccag
     3721 tggcgcgatc acgcacagcc ggttttcctt gatgccgatc tccattccca cgccttcaaa
     3781 cgccccggaa gtgtcttcca ggaatttttt tgaatcttcg gggttgaaaa acgaggtgta
     3841 aggatcttcc agcgccgcca ccattcccga aatcgcgccg tagatcattt tttgatgatc
     3901 aatttggtcc ttgtccacaa agttatcttc caaggcatgc catgtttgcc aaaaaagcga
     3961 catatccgcg tcggcgggcg gcagtttggg gcaactggag gttgcggtcc aattgccgat
     4021 ggcaaaactt gccaacgcgg tgacggcgat caaaaatccc caaaaatcac ggccttcctt
     4081 ttttttgttt tcattattta acattcccat attgaaatca catttgattt taacgactcc
     4141 caccactatt gcgggttcat gcgagcaaaa atctttcctg aattttttca aacaaattca
     4201 atctacacca taaatcgcgc aatcgcaaga gggcgtgccg tttagggcgc aatttgcaat
     4261 ttttatcttc cgcgattttg tgttatggtt tttcattatg atatggcgaa agcataacat
     4321 agcaaaaata tgacaaaaac cacaattgag tacggtgatt ttgaaaaatt ggatttgcgc
     4381 gtcggcaaga ttttggcggc aaatgaaccc gaatggagca ataaattgat tgaaatgaag
     4441 gttgattttg gatccgagat cggagaaaga acgattttgg cgggattgcg caaattcgtg
     4501 aattttgcgg atttgatcgg caacaaatat gtttttatca tcaatttgcc cgaacgcaaa
     4561 ctgggcgaag gggtcagcca aggaatgatg cttgccgccg gcggcgactc cacccacctt
     4621 cccctgcgcg ttgacgacgc aatcgccgaa ggctcgcaga tcagatagaa aatataaaat
     4681 aaaaaccgcc attcgggcgg atctttagca tttggacaat aaatcaaaat atgtttttct
     4741 gctcatatcg cacaatctca taaggttttt gatgacaaac actggaacat tttcatattt
     4801 cggtatcacc agcgggcgcg agcagttgtt ttttgtgtaa attaaatggt cgccgtttgt
     4861 gcgagcataa ttaaaaccag ctttttcaaa aacacatatt aaccgctgat aattaatcgg
     4921 cgttattctc ataccgccaa actcattttt tcaaacgcga ttatttcggg tgcttgttgt
     4981 cctgaaaatc ccgcttcatc cagaatttgg ttaagagtgt ccattttttg agcttcggaa
     5041 ataaataagc caacggcttc tttgatattt tttttcgctt catcaaccgt cttgccgcaa
     5101 gaagaaacgt ccagttgcgg cgcgtaagca acatacatat ctccttcttt ccatacttga
     5161 gtattaaatt gaattgtctt atttctcata ttttgattat gcccttatta tctcttttct
     5221 gatattttta taatttgtta ttggtcgttg ccgttagtta tctcggagaa gtctttttct
     5281 atttctttga attcgttttc cagcttgtcc aatttatctt tgctggattt tatcagcgcc
     5341 gccgcggttt tcactttttc caatcccgcc tccacgtcca aatcttcttg gccggaaaac
     5401 cacgccgaaa tctcggcaag ctcttttagg ttctcgctca aacttttctg attcggcggc
     5461 gtttgcgcgc cggcgctagt tttctttttc tcggccatat acttttttta tttgtgagtt
     5521 gattatacca tccgacaaaa caatgtccat atcgtcgccg gcttttgcgt cgccggcgcg
     5581 cttgatgatt gtgccgttaa tcttggcgat gcaatatccc aaccgcagtt gccgcgcggg
     5641 atcgcgggcg gcaatcaatt cctggaaatg ggcgattttt tcggcggcgc cggcaaggcg
     5701 gtttttgatt ttt
//
LOCUS       DAOQWE010000046         5688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_14459_length_5688_cov_8.686845, whole genome shotgun sequence.
ACCESSION   DAOQWE010000046 DAOQWE010000000
VERSION     DAOQWE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14459_length_5688_cov_8.686845"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1143
                     /locus_tag="PKK37_02360"
     CDS             103..1143
                     /locus_tag="PKK37_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR81253.1"
                     /translation="MASKSISPIISVAKKVCPAVITIVVSKDVSRVEGFYFVPLGDRQ
                     TVVPKINDSGEKTKIGGGSGFIISPDGYILTCSHVVDDVDASYTAIIDPEHKYECQVL
                     AKDPLIDLAILKIDARQKLPCLKMGDSEAIQLGESVLAVGNPMGEFDDTLSAGIVSGL
                     SRKITAYGGSKMQETSLRGLIQTDAAINPGNSGGPLVNMRAQAIGVNTATVMGAQNLC
                     FAIPVNYAKKMMEEVLKYGKLKRPFMGIRYVILNPEIAQANKLPVDYGALIVRETFGE
                     PAVIPGSAADLAGLKEYDIILEFGGQKISNERLLTDILQDCKIGDEIPMTVLRDGKIF
                     KLGIRLLERKNC"
     gene            complement(1223..1426)
                     /locus_tag="PKK37_02365"
     CDS             complement(1223..1426)
                     /locus_tag="PKK37_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HNR81254.1"
                     /translation="MKLTWLDITALVLVIVGGLNWGLAVFDFNLVNQLLGGVPTLETI
                     VYGVVGVAAIYVAMLVPKLSKGQ"
     gene            complement(1538..2614)
                     /locus_tag="PKK37_02370"
     CDS             complement(1538..2614)
                     /locus_tag="PKK37_02370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR81255.1"
                     /translation="MAKKILVISTRAGWGHIRAAQALEEYARQNLPEINVKHVDLCEI
                     EPRLGKFFEIFYDIASDYLPLAWGAVYQTFDKPPVSAAFRKAGGFHRLFRRRISNYLK
                     KESPDGVIFTNVIPAPMVAPPCKKIFPHVPLAVVVTDYHGHSYYNVPAIDRYFAPVPE
                     VKTDLARIGIPEDKIEVSGIPVGKKFYESYDREKIKKKLGIRNGFKTVVFVSRLSKEF
                     IIPALEDLLALDPPINLITVCGGNAGLYRKIKEQIGRRKNFKLINWTARLDEYMKSAD
                     VVVGKPGGLIVSECLALKKKMVLIDPIPGQEERNAEFIERHGYGRRARNAAQIAALVR
                     AADSQADKSFDGVDACAKILNYFR"
     gene            complement(2623..2955)
                     /locus_tag="PKK37_02375"
     CDS             complement(2623..2955)
                     /locus_tag="PKK37_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81256.1"
                     /translation="MRIVKEKFKTDQNNKLYWGRVYFISDDRQYKTKLIWAMSFEWVR
                     RQLLAEIRKDGDVDKLVNKIAVRKWKDTGRAVFLRQTQLDFWGAGDEENEFLRRFTKA
                     RFEADRCG"
     gene            complement(2962..4260)
                     /locus_tag="PKK37_02380"
     CDS             complement(2962..4260)
                     /locus_tag="PKK37_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81257.1"
                     /translation="MFEQLLAEQNKHWRGQKLEAGFERECLDDLMNYVATKHIIALLG
                     VRRCGKSTVIKQIINRLINNEKVCPKNILFLNLESPILENYKSDPNNLKRLFDEYKVA
                     AKPKKGRVFVFLDEAQFFPKWQVFVKDLYEKGGVKFFITGSNSRLLGAQMATLLSGRQ
                     IVKKIHPFNFQEIAAIKGIEAKEELAIAENGPALIGLCDEYLKMGGFPEIVLERNDAL
                     KRELLANYYRSILYQDIVPRFEIKKTKEVESLLLYLFSNIGQGYSYNSLGKFSQIQGK
                     TAKEYVGFFEESFLLSEISNYRYSLKKQENYPKKVYAVDNGFIETASFAFSENYGRLL
                     ENAVFAKLAATDKKLFYYRNSFECDFVVKGKIKIAAVIQATKSIQQTNEKREIKGVLE
                     AMKIFDLNEGTVITREQENERKIDGKIVRVIPLYKWLLAN"
     gene            complement(4435..>5688)
                     /locus_tag="PKK37_02385"
     CDS             complement(4435..>5688)
                     /locus_tag="PKK37_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNR81258.1"
                     /translation="LGEAHGKKIVKVIEMARQTGCPAIGIIDSGGARIQEGIASLDGY
                     AAIFNAMVKASGVVPQISVIVGPSAGGACYAPGLSDFIFMVDGIGQMFITGPEVVKKV
                     TGEEVTLEALGGASAHCAKSGCAHFPYANERECFAGVKKLLGYLPQNNVDDAPRSKGI
                     LSELFEKEESAKLAGIVPEEETKGYDVREVIAEVFDGNSFLEVQAGFAANAVVGLARL
                     NGHSLGVVANQPKIMAGVLDIDSSDKIARFVNFCDAFNLPLVNLVDTSGYLPGAAQEH
                     GGIIRHGAKVLYAYAAAAVPKVSVILRKAFGGAYIALASRQLGYDRVIAWPGAQIAVM
                     GPQQAAKIIYHREIAAAKNPEKAEAEKAEELREFVNPFAAAKLGQVDMVIHPKDTRVI
                     LIRLVESLLTKREPKIARKHGNTPL"
BASE COUNT         1431 a         1558 c         1204 g         1495 t
ORIGIN      
        1 aagtttagca aaaaatgcgc ttgttggcaa cggatttcaa tgctaaaata cacaaacctg
       61 agttttccga agaattgatt tttgattttt aaactttgaa ttatggcttc aaaatctatt
      121 tcccccatca tctccgtggc caaaaaggtt tgtccggcgg tgatcactat tgtggtttcc
      181 aaagacgttt cccgcgtgga aggcttttac ttcgtgcccc tgggcgaccg gcaaaccgtg
      241 gtccccaaaa tcaacgactc cggagaaaaa acaaagattg gcggcggttc ggggtttatc
      301 atttctcccg atggctacat tctcacctgc tcccatgtcg ttgacgacgt cgacgccagc
      361 tacaccgcca ttattgatcc ggaacacaaa tatgaatgcc aggtgctggc caaagatccg
      421 ctgattgatc tggcaatcct caaaatcgac gcccgccaga aattgccttg cttgaaaatg
      481 ggcgattcgg aagccatcca actgggcgaa tccgtgctgg cggtcggcaa tccgatggga
      541 gaatttgacg acacgttgtc ggccggcatc gtttcgggtt tgagccgcaa aatcaccgcc
      601 tatggcggca gtaaaatgca ggaaaccagc ctgcgcggac taatccaaac cgacgcggcc
      661 atcaatcccg gaaattccgg cggcccgctg gtcaatatgc gggcgcaagc catcggcgtc
      721 aacaccgcca cggtaatggg cgcacaaaac ctgtgctttg cgattccggt gaactacgcc
      781 aagaaaatga tggaagaagt gctgaaatac ggcaaactta aacggccgtt tatgggtatc
      841 cgctatgtga ttttaaaccc cgaaatcgcg caagccaaca aactgccggt ggattacggc
      901 gcactgatcg tgcgcgaaac tttcggcgaa ccggcagtga ttccgggaag cgccgccgat
      961 ctggccggct tgaaagaata tgatattatt ttggaattcg gcggccaaaa aatttccaat
     1021 gagcggctgt tgaccgacat tctgcaagat tgcaagatcg gcgacgaaat tcccatgacg
     1081 gtcttgcggg acggcaagat tttcaaactc ggcatccgtt tgctggaaag aaaaaactgt
     1141 taaaacctcc caagcaaaaa cggcctcccg gccgttttgt ttttcgtgtt tcccgcccct
     1201 caactcattt ctcattcact atttattgcc ccttgctcaa tttgggcacc agcatcgcca
     1261 cgtaaatcgc ggccacgccc accacgccat agacaatggt ttccagcgtc ggcacgccgc
     1321 ccaacaattg attcaccaaa ttgaaatcaa acaccgccaa tccccaatta agtccgccca
     1381 cgatcaccaa caccagagcg gtaatgtcaa gccaagtaag tttcataaat ttattttaaa
     1441 ttctaaatta acgaacactc gcccgacctt ttggttatta ttggattata caatatattt
     1501 tcggcaaatt aaaattgcca aggcaatgag cggcgcttta tctgaaataa ttcaaaattt
     1561 tagcgcacgc atcaaccccg tcaaaacttt tgtccgcttg agaatcggcg gcgcgcacca
     1621 gcgcggcaat ttgggcggca ttccgcgccc gccggccgta accgtgccgc tcaatgaatt
     1681 cggcgttcct ttcttcctgt cccggaatcg gatcaatcaa aaccatcttt tttttcagcg
     1741 ccaggcattc ggaaacaatc agcccaccgg gcttccccac caccacatcg gcgcttttca
     1801 tatactcatc aagccgggcg gtccaattaa tcaatttaaa atttttccgc cgtccgattt
     1861 gttccttgat tttgcggtat aggccggcat tgccgccgca aaccgtaatc aaatttatcg
     1921 gcggatccaa agccagcaga tcttccagcg ccggaatgat aaattctttg gaaaggcggc
     1981 tcacgaaaac cactgtctta aagccgtttc ttatgcccag tttctttttg atcttttccc
     2041 gatcatagga ttcataaaat ttcttcccca ccggaatgcc gctcacttca attttatctt
     2101 cgggaatgcc gatgcgcgcc aagtcggttt tcacttccgg caccggcgca aaataacggt
     2161 ctatcgccgg cacattataa taggagtggc cgtgatagtc cgtcaccacc accgccaaag
     2221 gaacgtgcgg gaaaattttt ttgcagggcg gcgccaccat cggcgcggga atgacattgg
     2281 taaagataac tccgtcggga gactcttttt tcaaataatt tgaaatgcgg cggcgaaaaa
     2341 gccggtgaaa cccgccggct ttgcggaagg ccgccgaaac cggcggtttg tcaaaagtct
     2401 ggtaaaccgc gccccacgcc aacggcagat agtcgctggc aatgtcatag aatatttcaa
     2461 aaaacttccc cagccgcggc tcaatctcgc acaaatcaac atgcttgaca ttgatttccg
     2521 gcaaattttg gcgcgcgtat tcttccagcg cttgcgccgc acggatatgc ccccagcccg
     2581 ccctcgttga aatgaccagt attttttttg ccataaagtc cgttatccgc aacgatcggc
     2641 ctcaaacctt gctttggtga atcgtcgcaa gaattcgttt tcttcgtcgc cggcgcccca
     2701 aaagtccaat tgggtttgcc gcaaaaaaac cgcccgcccc gtgtctttcc atttgcgcac
     2761 cgcgattttg ttgaccagtt tatcaacatc gccgtctttc cggatttccg ccagcaactg
     2821 ccgccgcacc cattcaaaag acatcgccca gatcaatttc gttttatatt ggcgatcatc
     2881 ggaaataaaa tataccctcc cccagtagag cttgttgttt tggtccgtct tgaatttttc
     2941 cttgacaatc ctcatcaaat tttaatttgc caaaagccat ttatataacg ggatcactct
     3001 cacaattttt ccatcaattt ttctttcgtt ttcttgttct cgcgtaatta ccgtcccctc
     3061 gtttaaatca aaaattttca tcgcttccaa aacgcctttt atttctcgtt tttcatttgt
     3121 ttgctggatg cttttagtcg cttgaatcac cgcggcaatt tttattttcc ctttaaccac
     3181 aaaatcacat tcaaacgaat ttctgtaata aaacaatttt ttgtccgtag cggcaagttt
     3241 cgcgaatacc gcgttctcca atagacggcc ataattttcg ctgaaagcaa atgacgccgt
     3301 ttcaataaat ccgttatcaa ccgcgtaaac ttttttgggg taattttcct gtttttttaa
     3361 tgaataccgg taattggaaa tttccgacaa taaaaacgat tcttcaaaaa aaccgacata
     3421 ttctttggcg gttttcccct ggatttggga aaatttgccc agtgaattat agctgtaacc
     3481 ttgcccgata ttggaaaaaa gatataaaag caagctttcc acttctttgg ttttcttaat
     3541 ctcaaaccga ggaacaatgt cttgatataa gatgctccga taataattgg ccaacagttc
     3601 ccgctttaat gcatcatttc gctccaaaac tatttcggga aatccgccca tctttaaata
     3661 ttcatcgcaa agaccgatta gagccgggcc attttcggcg attgccaact cctccttggc
     3721 ctcaattcct ttaatcgcgg cgatttcctg aaaattgaaa gggtggattt ttttgactat
     3781 ttgccgcccg gaaagcaagg ttgccatttg cgcccccaac agccgagaat tggaaccagt
     3841 gataaaaaat ttcacaccgc ccttttcata gaggtctttg acaaacactt gccatttggg
     3901 aaaaaattgc gcctcgtcaa gaaaaacaaa cacacgcccc ttcttgggct tggccgcgac
     3961 tttatattcg tcaaacagcc gtttcaaatt gttgggatcg cttttataat tttccagtat
     4021 cggcgattcc aaattcaaaa aaagaatatt tttgggacaa actttttcat tgtttatcaa
     4081 tcggttgata atctgtttta tcaccgttga cttgccgcaa cggcgcactc ccagcaaagc
     4141 gatgatatgc ttggtggcaa catagttcat caaatcatca aggcattcac gctcaaaacc
     4201 ggcctccaat ttttggcccc gccaatgctt gttttgctcc gccaataatt gctcaaacat
     4261 aataattggt aatgtattac gaattaaacc catttaattc gtaatatact acgaattaac
     4321 cgcaagtcaa cccgccgcct gtgaatgttt gcttcgtttt gattttttaa ctttgaattt
     4381 tgaatttgtt tgggaattga aaattggaaa ttggtcattc cactttgatg gttatcaaag
     4441 cggcgtgttt ccgtgcttgc gggcgatttt gggttcgcgc ttagtcaaca gcgactccac
     4501 cagccggatc agaatcacgc gcgtgtcttt gggatgaatc accatatcca cctgcccgag
     4561 tttggccgcc gcgaacggat tgacaaattc gcgcaattct tcggcttttt cagcttcggc
     4621 tttttcggga tttttggccg cggcaatctc gcgatggtaa ataatcttgg cggcctgttg
     4681 cggccccatc accgcgattt gcgctcccgg ccaggcaatc acccgatcat aacccagttg
     4741 gcggctggcc agcgcgatgt aagcgccgcc gaacgccttg cgcaaaatca cgctcacttt
     4801 gggcaccgcc gcggcggcat aggcatataa gactttcgcg ccgtgccgaa taatgccgcc
     4861 gtgttcctgc gccgcgcccg gcaaatagcc ggaagtatcc accagattga ccagcggcag
     4921 attgaatgcg tcgcaaaaat tcacgaaccg cgcaattttg tccgaggaat caatatccaa
     4981 aacgccggcc ataattttgg gctggttggc caccacgccc agcgaatggc cgttcagccg
     5041 cgccaatccc accaccgcgt tggcggcaaa tcccgcctgc acctccaaaa acgaattgcc
     5101 gtcaaatacc tcggcaatca cctcccgcac atcataaccc ttggtttcct cttcgggaac
     5161 aatgccggcc agcttggcgc tctcttcctt ttcaaacaat tccgacagga ttccttttga
     5221 acgcggtgca tcgtcaacgt tgttttgcgg caaatagccc aaaagttttt tcactccggc
     5281 aaaacattcc ctttcattgg catatggaaa atgggcacag ccggacttgg cgcagtgcgc
     5341 cgacgctccc ccgagcgcct ccagcgtcac ttcttcgccg gtaacttttt tcaccacttc
     5401 cggaccggtg ataaacattt ggccgatgcc gtcaaccata aaaatgaaat ccgacaatcc
     5461 cggcgcatag cacgcgccgc cggccgacgg accgacaatc accgaaattt gcggcaccac
     5521 ccccgacgct ttgaccatgg cattgaaaat cgccgcatac ccgtccaaac tggcgattcc
     5581 ctcttgaatg cgcgcaccgc ccgaatcaat gatgccgatc gccggacaac cggtctggcg
     5641 cgccatttca attactttga caattttttt gccgtgcgcc tctcccag
//
LOCUS       DAOQWE010000047         5681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_14488_length_5681_cov_8.738358, whole genome shotgun sequence.
ACCESSION   DAOQWE010000047 DAOQWE010000000
VERSION     DAOQWE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14488_length_5681_cov_8.738358"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2323
                     /locus_tag="PKK37_02390"
     CDS             <1..2323
                     /locus_tag="PKK37_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNR81259.1"
                     /translation="DFHKLFQNNWYISDWQYQSWKTKYEYLAKIANPDILKLKTDNPI
                     KKSAISFTRAWTNGRKLFIDDFNDKFILQESPRIKQLLDNYKIPCNADQIRAIASDEN
                     NTLLIAGAGTGKTTTILGKIIYLIKRAKIAPQEILVLSFTGNAVEELKERIAEKFSGD
                     KIEILTFHSFGFSIIGNVLGRKPDLAFPESSDKKIFLGRQLDLLLEKRDYLSRAIEYF
                     AYYFRPVNLKPEFEDFNEYFNYVKTEQNLTLKKERVKSYQEVMIANFLYINGINYEYE
                     KPYEHKTSSVKYRQYFPDFYLPDHDIYIEHFGIDRKGKIHFTKNREQNLIHSKKYQSG
                     MEWKRNLHKEHKTKLIETYSYEFQERNWREMLTKKLDKYGIKYSPRNIKEILFTLRSS
                     DKIKQIVELIATFMDLCKSNGYNLSELEEIIKKRDNLRELAFFEILSPIFTKYENYLK
                     QNNRIDFHDMLIKATQFVQENRYKAKFKYIIIDEFQDFSVSKRDLIKALCKQNQNTKL
                     FCVGDDWQSIFRFAGSDISLMKNFEQSYGFTRHNKLTTTNRFNNQLATISNQFILKNP
                     NQIEKQLKSEKNIVGDAIKIFSKKKNGDIERFLDEIIGSLDKRALENKKTATVFLLGR
                     YKHNIPQKFAQLRQQYKNLRIKYLTVHAAKGGEADYVIIMDVISGKYGFPAEIADDPL
                     LGIVLSQSDPYPYAEERRLMYVAMTRARNKVFVITENGVRSLFVAELEEFDPKSSAVH
                     CKECGGKMVIRQSKYGAFYGCGNFPKCNYTESA"
     gene            2618..3685
                     /gene="ychF"
                     /locus_tag="PKK37_02395"
     CDS             2618..3685
                     /gene="ychF"
                     /locus_tag="PKK37_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNR81260.1"
                     /translation="MSLHVGIVGLPNVGKSTLFKAITKKQAECANYPFCTIEPNIGVV
                     PVADERVDKLAELTKSAKKIFATVEFVDIAGLVKGAAQGEGLGNKFLQNIRETDAILY
                     ILRAFGNEKIINTQNSIDPWRDKEILDTELALKDLETALKRLESVESEVRGNKPGAKE
                     EMQALQKAKSFLEAGKILSEAEFTEEEKKNINRLQLLTMKPRLYVLNGNNDEIGKDIA
                     EKFEEKKLPHIVIDILGESDGEGLTEAEKEDFNLPSGENLDLLIKKSYELLELITFFT
                     TGPDETRAWTLKRGSAAPKAGGVIHTDFEKKFIRAEVINWQDLLDAGGFSQAREKGLL
                     RTEGKEYIVQDGDVIEIKANA"
     gene            3734..4351
                     /locus_tag="PKK37_02400"
     CDS             3734..4351
                     /locus_tag="PKK37_02400"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HNR81261.1"
                     /translation="MALAIISVIGVVLTGAVVVFVQYQSSKVPVMSGKTANHSSAATT
                     EPVKTEEKNENEENGVKNFQECVAAGNQVEEIYPARCFAPDGKTFTQNIGNEMKLAEL
                     IMIYSPRPNAQIASPLVISGKARGNWFFEASFPVKLMDENGNELANGIAIAQSDWMTE
                     DFVEFLATLEFTAPPTPAGTLILQKDNPSGLSENDNQLIVPVEFE"
     gene            complement(4407..5384)
                     /locus_tag="PKK37_02405"
     CDS             complement(4407..5384)
                     /locus_tag="PKK37_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR81262.1"
                     /translation="MNNSDKPVIAHIPDFLDYCEIEKGLSENTQINYRRYLQKFINWL
                     KSQKKEQILPHELSADDVWAYRVHLSRFIDPQGGNLSKSTQNYYLIALRALLGYFTAK
                     DIAALPADKIKLPKDARKEKTVKFLNLEQIERLLLAPDENTDAGVRDRAILETLFSTG
                     LRIAELVNLNREQFANLQENKELELGIIGKGKYPRTVYFSARALGWIKKYLKIRDEIC
                     EKEEKALFVNFRARKNAPRRLTARSIERMVKNCAIGANVPLFTTPHTLRHSMATDLLT
                     QGADLRAIQEFLGHRSIMTTQIYTHVTNKRLRDIHRQFHSGRKLKDGKQ"
BASE COUNT         1849 a         1153 c         1220 g         1459 t
ORIGIN      
        1 agattttcac aaactttttc aaaataattg gtatataagc gattggcaat atcaatcttg
       61 gaaaacaaaa tatgaatatc tggctaaaat tgcaaatccg gatattttaa aactcaaaac
      121 cgataatcca ataaaaaaat ccgctatttc ctttactcgg gcgtggacca atggaagaaa
      181 actattcata gatgatttca atgataaatt tatcctgcaa gaatcgccgc gcataaaaca
      241 actgcttgat aattataaaa tcccctgcaa cgccgatcaa attcgggcaa ttgcctcgga
      301 cgagaacaac actttattga tcgcgggcgc gggcaccgga aagacaacca caatcctggg
      361 aaaaataatt tatcttatta aaagagcgaa aatagcaccc caagaaatat tggttttatc
      421 atttaccggc aacgcggtgg aagaattaaa agaacgaatt gctgaaaaat tttcaggaga
      481 taaaatagag attctgacat tccatagctt cggattctcc ataatcggca atgtcttggg
      541 acgcaaaccg gatttagcgt ttccggaaag ttcggataaa aaaatatttt tgggccgcca
      601 attagattta cttttagaga aacgagatta tcttagtcgc gcgatagaat attttgctta
      661 ttattttcga ccagtaaatt tgaaaccgga atttgaagat ttcaatgaat actttaatta
      721 tgtaaaaacg gaacaaaatc taactttaaa aaaggagcgt gtaaaaagct atcaggaagt
      781 aatgattgct aattttttat atattaacgg aatcaattac gaatacgaga agccatatga
      841 gcataaaacc tcaagcgtca aatatcggca atatttcccg gatttttatc ttcccgacca
      901 tgatatctac attgaacatt ttggcattga tcgcaaggga aaaatacatt tcacaaaaaa
      961 tagagagcaa aaccttattc attcaaaaaa atatcagtcc ggcatggaat ggaagcgaaa
     1021 tttgcacaaa gaacataaaa ccaaattgat agaaacatac agttatgaat tccaagaaag
     1081 aaattggaga gaaatgctta ccaaaaaact tgataaatat ggcattaaat acagtcccag
     1141 aaatatcaaa gaaatactct ttaccctaag aagtagcgat aaaataaaac agattgttga
     1201 attgattgcc acttttatgg atctttgcaa atcaaatggc tataatctgt ccgaattgga
     1261 agaaataatt aaaaaacgcg ataatctcag ggaattggca ttttttgaga tcctttcacc
     1321 catatttacc aagtacgaga attatctgaa acaaaacaac cgcatcgatt ttcatgacat
     1381 gctaatcaaa gcaacccaat ttgttcaaga aaatcgttat aaagcgaaat tcaaatacat
     1441 aattattgat gaatttcagg atttttccgt aagcaagcgc gatcttatca aagctttatg
     1501 caagcaaaac caaaacacca agctattttg cgtgggcgat gattggcagt caatatttag
     1561 atttgccgga tcagatattt cattgatgaa aaactttgaa caatcttatg gctttacgcg
     1621 ccataacaag ctcacaacca ccaatagatt taataaccag ctggccacca tctcaaatca
     1681 atttatattg aaaaatccga atcaaattga aaaacaatta aaatcagaga aaaatattgt
     1741 cggtgatgcc ataaaaatat tcagtaagaa gaaaaatgga gatattgagc gttttttaga
     1801 cgaaattatc ggatctctgg acaaaagagc tctggagaat aaaaaaacgg caacagtttt
     1861 cctgcttgga cgatataagc ataatattcc gcaaaaattc gcccaattgc ggcaacaata
     1921 taaaaatttg agaataaaat acctaactgt tcacgcggca aagggcggtg aagctgatta
     1981 tgtaattatt atggatgtaa tttccggcaa atatggattc ccagccgaaa ttgccgacga
     2041 ccccttgcta ggaatagttt taagccaatc tgatccatac ccttatgccg aagaaagacg
     2101 actgatgtat gtggcaatga ctcgagctcg caataaagtc tttgtcatca cggaaaatgg
     2161 cgtacggtct ttgttcgttg cggaattgga agaatttgat ccgaaatcat ccgctgtgca
     2221 ctgcaaagaa tgcggcggca aaatggttat cagacaaagc aaatatggcg cattttacgg
     2281 ttgcggtaat ttcccgaagt gcaactacac cgaatcggca tgaaaataaa ctacgccggg
     2341 gcagagcccc gacgtattcc ggcgctccgc ttcgctacgc gatcggaaaa gtttgtttcc
     2401 ccgccattca tccccggggc aaagccccga ggtattctgg cgggtaaata aaaaacgaac
     2461 aacagccgtt aaaaacggct acatcaaatt ttatatttat gcaattatat cttctgtgat
     2521 tttatgattt tataattaca tttatatagt cgcgtgattc tgtgattttg cattttggca
     2581 attatatgtt tatatgattt tatgattaaa tgattttatg agcttacacg tgggaatcgt
     2641 gggtctgccc aatgtgggca aatccacact atttaaagcc atcaccaaaa aacaggccga
     2701 atgcgccaac tatccgtttt gcaccattga gccgaacatc ggcgtggtgc cggttgccga
     2761 cgaacgcgtt gacaagctgg ccgaattgac caaatcggcg aaaaaaattt ttgccaccgt
     2821 ggaattcgtt gacatcgccg ggctggttaa gggcgccgcg caaggggagg gtctgggcaa
     2881 caaatttttg cagaatatcc gtgaaaccga cgcgattttg tatattttgc gcgcttttgg
     2941 caacgaaaaa atcatcaaca cgcaaaattc aattgaccct tggcgcgaca aggaaatttt
     3001 ggacaccgaa ttggcgttga aagatttgga aacggcgtta aagcggcttg aatccgtgga
     3061 aagcgaagtg cgcggcaaca agcccggcgc caaagaagaa atgcaggctc tgcaaaaagc
     3121 gaagtcattt ttggaagcgg gaaaaatatt gtccgaagcc gaattcaccg aagaggaaaa
     3181 aaagaatatc aatcgcctgc aattgctcac aatgaagccg cgcctttatg ttttaaacgg
     3241 gaataacgat gaaatcggaa aagatatcgc cgaaaaattt gaagaaaaaa aattgccgca
     3301 tatcgtgatt gacatcttgg gggaaagcga cggcgaaggc ctcaccgaag cggaaaagga
     3361 agatttcaat ctgccttcgg gagaaaatct ggatttgctg ataaaaaaat cctacgagct
     3421 tttggaatta atcacttttt tcaccaccgg accggatgaa acccgcgcct ggacgctaaa
     3481 aaggggaagc gccgccccga aagccggcgg agtgatccac accgattttg aaaaaaaatt
     3541 cattcgcgcc gaagtgatta actggcagga tctgcttgac gccggcggat tttcccaagc
     3601 gcgcgaaaaa gggcttttgc gcaccgaagg caaagaatat attgttcaag acggcgatgt
     3661 gatcgaaatc aaagctaatg cgtaaaaaaa gtttagggtt tagggtttaa aacatatgaa
     3721 tcaaaaagaa attatggcat tggccattat ttcggtgatc ggggttgtac ttaccggagc
     3781 ggtggttgtt ttcgtgcaat atcaatcctc aaaagtgccg gtaatgtccg gtaaaacggc
     3841 aaatcattca agcgcggcaa ccactgagcc ggtcaagacc gaggaaaaga atgaaaacga
     3901 agaaaacgga gttaaaaatt ttcaagaatg cgttgccgcc ggcaatcagg tggaggaaat
     3961 ttatccggca agatgctttg ctccggacgg aaagacattc acgcaaaaca ttggcaacga
     4021 aatgaaattg gccgaattga taatgatcta ttcgccgcgc ccaaacgccc aaatcgccag
     4081 cccgctcgtc atcagcggca aagcccgcgg caactggttt tttgaagcgt ctttcccggt
     4141 aaaattaatg gatgaaaacg ggaatgaatt ggcgaatggc attgccatcg cgcaaagcga
     4201 ttggatgacc gaggattttg ttgaatttct ggccacgctt gaatttacgg ccccgccgac
     4261 gcccgccggc accttgattt tgcaaaaaga caatccgtca ggactttccg agaacgataa
     4321 tcagctaatt gtccccgtag agtttgaata aaatattgtg cgacatttat gatgacaaaa
     4381 accgcttcag cggtttttat gtttgtttat tgtttgccat ccttcaattt cctgccgctg
     4441 tgaaattggc ggtgaatgtc gcgcaaacgc ttgttagtca cgtgggtgta gatttgcgtg
     4501 gtcatgatac tgcggtgccc caaaaattcc tggatggcgc gcaaatccgc gccctgcgtc
     4561 aaaagatcgg tggccatgga gtggcgcaga gtgtgcggcg tggtaaacag cggcacattg
     4621 gcgccaatcg cgcagttttt caccattcgc tcaattgacc gggcagtcaa ccgccgcgga
     4681 gcgtttttgc gcgcccgaaa attaacaaac agcgcttttt cttccttttc acagatttca
     4741 tcgcggattt tcaaatattt tttaatccat cccaaggcgc gggcggaaaa ataaaccgtc
     4801 cgcggatatt tgcccttgcc gataatcccc agttcaagct ctttgttttc ctgcaaattc
     4861 gcgaattgtt cgcgattcaa atttactaat tcggcgattc tcaatccggt ggaaaacagc
     4921 gtttccaaga tcgcgcggtc gcggacgccc gcgtcggtgt tttcatccgg cgccagcaaa
     4981 agcctttcaa tctgttccag atttaaaaat tttacggttt tttcttttcg cgcatctttc
     5041 ggcagtttaa tcttgtccgc cggcaaggcg gcaatatcct tagccgtaaa atagcccaac
     5101 aaggcacgca aagcaatcag gtagtagttc tgggtggatt tggataaatt gccgccctgc
     5161 gggtcaataa aacgggataa gtgcacccga taggcccaaa catcgtcggc agacagttcg
     5221 tggggcaaaa tctgctcctt tttttgggat ttgagccaat tgatgaattt ttgcaggtac
     5281 cggcggtaat ttatttgggt gttttccgag agcccttttt caatctcgca ataatcaaga
     5341 aaatcgggga tatgggcaat gacgggtttg tcggagttat tcattggttt tagaattaaa
     5401 cagggaacgt taattgtgtt taaatagaat aaaagagaaa aacaaaaaaa taaaattgaa
     5461 aaaatatatt tgaagcaaaa tgaaattaaa aaatcttcaa aaaaaacaac aagctttccg
     5521 gcagggaagg cttccggcaa acccccgaaa agaaaacgac aagcaaccct caaaaatcgc
     5581 aaaatccgct taaatgccgc caatcggcca aaatcccgat gttaacgtcc attaaccttg
     5641 attgtgcgac atttaatata ttgacgacga gcgaatagct c
//
LOCUS       DAOQWE010000048         5680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_14495_length_5680_cov_6.352178, whole genome shotgun sequence.
ACCESSION   DAOQWE010000048 DAOQWE010000000
VERSION     DAOQWE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14495_length_5680_cov_6.352178"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..882
                     /locus_tag="PKK37_02410"
     CDS             250..882
                     /locus_tag="PKK37_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81263.1"
                     /translation="MFEPAGARALEYLHSRGLTDTTIKTFRLGFSPKFNQGDLYIPHG
                     ITIPAFANGVLWYVKIRTNGEPKYKLVAGSKPAAIFNADNLATARDCLLVEGEFNAMI
                     GHQTINDVIAVASMGSAGNRPDLATWGPYFINKTFIFTLYDADDAGQSGALALHNQLG
                     ERVKLVALPANAGDLNDYYTAGGDVWQWMKTCFEFWEPATDNAGKENNAE"
     gene            872..1264
                     /locus_tag="PKK37_02415"
     CDS             872..1264
                     /locus_tag="PKK37_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR81264.1"
                     /translation="MQNNAEKNAHLKPKQRAAIVALLSEKTKRDAAQSAGISERQLYT
                     WLQNPIFNAALAEAEADARGQIRRQITNRAAAIADVMAEIMENPDVTPAVRLQAAAKL
                     GDLFIKTTDDADVIRRITALEVVQYERE"
     gene            1251..1496
                     /locus_tag="PKK37_02420"
     CDS             1251..1496
                     /locus_tag="PKK37_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81265.1"
                     /translation="MSGNKPKKKPKTINARLDELENHAGDDDENNEPALNICWHCIGD
                     PEPVHEPGTLVIEWGENDEFISYRVPSDGKKKHNNPH"
     gene            1465..1701
                     /locus_tag="PKK37_02425"
     CDS             1465..1701
                     /locus_tag="PKK37_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81266.1"
                     /translation="MAKKSITTRIEALENFRSKKQTMIVLHQDLYNPDLFHYGKGPTA
                     EIITRAEADRRFSGNEYLVLLICYDKTIPGDDFS"
     gene            complement(1895..2305)
                     /locus_tag="PKK37_02430"
     CDS             complement(1895..2305)
                     /locus_tag="PKK37_02430"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR81267.1"
                     /translation="MIKPTQAAKLLDVNDSTVRRWAVRFARHLSPQTPGKARVYTNAD
                     LDVFRRIKEFTASGKTLAQIDDLLLYAPEDDAGNSTGLIPWADAIKSIETQRAIMAQL
                     QSQIDAQNQRLAMLENWLALPWYKRIGKTPPTNH"
     gene            2553..3527
                     /locus_tag="PKK37_02435"
     CDS             2553..3527
                     /locus_tag="PKK37_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR81268.1"
                     /translation="MDAIIKTDNNNHDLTRLDENPAAVYLKSLRPSGQRSMHGCLAKM
                     ADIFTNGQITNPLYFPWHLLKYQHAQAMKTSLLDLPEIKSPATVNKYLAAIKGVAREA
                     WNLGIMATDDYIRIKSIKPVIGETIPAGRYVTSGEIMALLKQCQNEPGPAGVRDAAII
                     GIMVAGGLRRDEIIKLDFADFHAANGELFVTGKRGKNRMVPLANGALDALLDWLHLRG
                     NEPGPLFWPINRGGRMQNKRITSQAVYLMLKNRSEISGVPALSPHDLRRTAASDLLVN
                     NDALTVAKMLGHSNLDVTMRYDRRGDVAKRDAAKTLFIPYTRKFGDDK"
     gene            4376..5416
                     /locus_tag="PKK37_02440"
     CDS             4376..5416
                     /locus_tag="PKK37_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR81269.1"
                     /translation="MADITQPQPPTQYLAAGLLELPSVNIFYGAPGTLKSFLIADLLV
                     CIAAGKTWLEPAPWQTGGQSFPTIKNPVMWIDFDMGRRRTIERFAALAKHYKTPTDAP
                     ITIYSMSNPPLDASNPGHIAELVARVATTGAKLIVFDNLGTISGGIEENSSAMIGVMF
                     NLRWLADVTGAAIVIIHHQRKGNGIGGRAGDALRGHSSIEAAIDLALQITREPYAELI
                     NIEMTKSRGLEVYPFSAAFTFDHNNAGDLETAAFYSIEPDDKQSNRAIEMAIKDALKS
                     GGMIQTELWKAVKDALPDVTKNRILDYIKRMVAKNDLNMVPGINNSRVYTLPVSRFST
                     VSRGFPENRTDN"
     gene            5494..>5680
                     /locus_tag="PKK37_02445"
     CDS             5494..>5680
                     /locus_tag="PKK37_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HNR81270.1"
                     /translation="MGFDGLKNKIQIIDLVDGLKRVASSGGGEFAGPCPFCGGIDRFR
                     VQPAKNIWLCRHCTNGIW"
BASE COUNT         1635 a         1203 c         1432 g         1410 t
ORIGIN      
        1 gtgcagccgg ccaaaaacat atggttatgc cggcattgca caaacgggat ttggcgggat
       61 gttattgatt ttattgcccg gcgtgataac gtaaccatta gccaggcggc ccaatccatt
      121 gatggtggtt ttccgcggtt ttccaaacaa tctggaaaac taaaaaaacc ggtttaccat
      181 gcatacaagc cccccacgga tgcatggcaa gcagctgcac gggaagccgt ggccatttgt
      241 gaaaacaatt tgtttgaacc ggccggggcg cgtgcattgg aatatttgca cagccggggg
      301 ttaacagata caaccatcaa aacattccgg ttggggtttt cccccaaatt caaccagggt
      361 gatttataca tcccccacgg cataaccatc ccggcatttg ccaatggtgt tttgtggtat
      421 gtaaagatta gaaccaacgg cgaaccaaaa tataaattgg ttgccggaag taaaccggcg
      481 gccattttca atgcagataa tttggccacg gcgcgggatt gtttactggt tgaaggggaa
      541 ttcaatgcca tgattggcca tcaaaccatc aatgatgtga ttgccgttgc atcaatgggg
      601 agcgcgggaa accggccgga tttggcaacg tgggggccgt attttataaa caaaacattt
      661 attttcacgt tgtatgatgc ggatgatgcc gggcaatctg gtgcattggc cttgcataac
      721 caattggggg aacgcgtgaa attggttgca ttgccggcaa acgccgggga tttgaatgat
      781 tattacacgg ccggcgggga tgtttggcaa tggatgaaaa catgttttga attttgggaa
      841 ccggcaaccg ataatgccgg gaaggaaaat aatgcagaat aacgcagaaa aaaacgcaca
      901 tttgaagccc aaacaacggg cggcaatcgt tgcattgcta tcagaaaaaa ccaaacggga
      961 tgcagcccaa tcggccggca ttagcgaacg gcaattatat acatggttgc aaaacccaat
     1021 tttcaatgct gcattggccg aagccgaagc cgatgcccgc gggcaaatac gccggcaaat
     1081 aacaaaccgg gcggcggcca ttgccgatgt tatggcggaa atcatggaaa acccggatgt
     1141 tacgccggcc gtaaggttgc aagcagctgc aaaattgggg gatttattta tcaaaaccac
     1201 ggatgatgcc gatgttatcc gccgcattac tgcattggaa gtggtgcaat atgagcggga
     1261 ataaaccaaa aaaaaagccc aaaaccatca atgcccggtt ggatgaattg gaaaaccatg
     1321 ccggcgatga tgatgaaaac aatgaaccag cattaaacat ttgttggcat tgtataggcg
     1381 accccgaacc cgtgcacgaa ccgggcacgc ttgttattga atggggggaa aacgatgaat
     1441 tcatttcata cagggtgccc agcgatggca aaaaaaagca taacaacccg cattgaagcg
     1501 ttggaaaatt tccgcagcaa aaaacaaaca atgattgttt tgcatcaaga tttgtataac
     1561 ccggatttat tccattacgg caaggggcca acggccgaga taattacacg cgccgaagcc
     1621 gaccggcggt tttccggaaa cgaatatttg gttttattga tttgttacga taaaaccatc
     1681 cccggggatg attttagtta attaggcaat cacaagcaaa aaaacggggt tttggcggtg
     1741 ttattggggc ataaggataa tttacttata tgccccaaat aaaccgttgc aagggtgtaa
     1801 tttttgaatc acaagcccaa attttacgtg gcggattatt gggaacggat tgcagcaatt
     1861 caattcaaac gatttgaaca acccccccag gccatcaatg atttgttggc ggtgttttcc
     1921 caatgcgttt ataccagggc aatgcaagcc aattttcaag catggcaagc cgttggtttt
     1981 gggcatctat ttgtgattgc aattgggcca tgattgcccg ttgcgtttca atggatttga
     2041 tggcatcggc ccatggtatc aagccggttg aattgccggc atcatcttcc ggggcatata
     2101 gcagcaaatc atcaatttgg gcaagcgttt tgccggatgc cgtaaattct ttaatccggc
     2161 ggaaaacatc caaatcggca ttggtataaa cgcgggcctt gccgggtgtt tggggggaga
     2221 gatgccgagc aaaccgaacg gcccaacggc gaacggttga atcgtttaca tccaataatt
     2281 ttgctgcttg cgttggtttt atcataagcg gttttgtgca cggtttttcc aggcaaaccc
     2341 gattataagc ggtgccgtgc acgttgtaaa tattgggggg aaaatcaaat caatttgatg
     2401 atgccggcat catgcacggt tttgtgcacg gttgccaata aggccccgca gctgcaaaat
     2461 tggtgcttga catgctatta tatttcgtgt atattactta ttagaataat acttctaaat
     2521 aataaacaat aaaaatgaat ggatggccaa aaatggatgc aataatcaaa acagataata
     2581 acaatcatga tttaacccgg ttggatgaaa accccgcagc cgtttatttg aaatctttac
     2641 ggccatccgg gcaacgttct atgcacggat gtttggcaaa aatggccgat atatttacca
     2701 atggccaaat aacaaacccg ttatatttcc catggcattt gttgaaatat cagcatgcac
     2761 aagccatgaa aacatccttg ttggatttgc cggagataaa aagcccggca accgtaaaca
     2821 aatatttggc agccatcaag ggtgttgccc gtgaagcatg gaatttgggc atcatggcaa
     2881 cggatgatta catccggata aaatccatca aaccggtgat tggggaaact atccccgcgg
     2941 gccgttatgt tacatccggc gaaatcatgg cgttattgaa acaatgccag aatgaaccag
     3001 gcccggccgg cgtgcgggat gccgccatca ttggcatcat ggttgccggt ggtttacggc
     3061 gtgatgaaat catcaaattg gattttgcgg attttcatgc cgccaacggt gaattgtttg
     3121 ttactggtaa acgtggcaaa aatcgcatgg tgccattggc aaacggcgcg ttggatgcat
     3181 tactggattg gttgcatctt cgtggcaatg aaccagggcc attgttttgg ccaatcaacc
     3241 ggggcgggcg tatgcagaat aaacgcataa catcgcaagc ggtttatttg atgcttaaga
     3301 accgttcgga aatttccggg gtgccggcat tatcccccca tgatttacgg cgaacggccg
     3361 caagtgattt acttgtaaac aatgatgcat tgacggttgc caaaatgttg ggccattcca
     3421 atttggatgt aacaatgcga tacgaccggc gcggagatgt tgccaaacgt gatgccgcca
     3481 aaacgttatt tattccatat accaggaaat ttggggatga taaataaacc agtgattgca
     3541 cgtgatataa gccccaaaaa tgcgggtttt acggcgtttc taccagggta tagtataaaa
     3601 tcccgtgaaa atttcccagt tacaattaac acaaggcatt tgaattgtta gttttgttaa
     3661 aatacagtat gttttaagta atctatacgc aaacttgact ggataaaata tattgatggt
     3721 gttactatca atttagaaca tttacattgg aagccccaat taaaagccga agccatggca
     3781 ggaaccatgg cagcgggcgg ggtgcttgcc gggcattgac aagcatatat ataaaatata
     3841 tagcacttaa gtaaaaaaaa gtaaagtaaa tttagcagcc cggtataagc gtaatgaacg
     3901 taaccgggtt tttttgttta attttgcgag gtgcaacggg tgttatataa ccgaatgttt
     3961 accatgaaaa caacccccca atggttggga atgttggggg atttggcgaa ggccgccgac
     4021 cgtaaacgat gcgattattt gcgggatttg attaatttgc tgcatacgaa cccaaacgcg
     4081 cggcaaacca taacggatta tttgcggggt gcttatgccg gaaaccatta acgaaaatgc
     4141 cccggcggga gggccagggc aaacgaacca aagacttgca aacagcaatc aacatgggga
     4201 ttatatccca aaacaatcta tcaatcaatt tgcgggggat gaattaacca aattggccgc
     4261 caaatatttg acgtttgatg atgccggcaa ggcccaattc aaccattttg ttttgcatcc
     4321 ggatgaattg ccggccgaag atgtaaccac gggggatgga tggcagccaa taacaatggc
     4381 cgatataacc caaccccaac ccccaaccca atatttggca gctggtttat tggaattgcc
     4441 gagtgttaac atcttctatg gcgcgccggg aacgttgaaa tcgtttctaa ttgccgattt
     4501 gcttgtttgc atcgcagccg gcaaaacgtg gttggagccg gccccgtggc aaacgggcgg
     4561 gcaatcattc ccaaccatca aaaacccggt gatgtggatt gattttgata tgggccgccg
     4621 gcgaaccatt gaacgatttg cagcattggc caaacattac aaaaccccca cggatgcccc
     4681 aataacaatt tacagtatga gcaacccgcc gttggatgca agcaatcccg gccacattgc
     4741 ggagctggtt gcacgcgttg ccacgaccgg tgccaaattg attgtgtttg ataatttggg
     4801 aaccattagc gggggcattg aagaaaattc aagcgcgatg attggcgtaa tgtttaattt
     4861 gcgatggttg gcggatgtaa ccggcgcggc cattgtaata atccatcatc aacggaaggg
     4921 caacggaatt gggggccggg ccggcgatgc attgcggggc catagcagca ttgaagccgc
     4981 catagatttg gccttgcaga ttacacggga accctatgcg gaattgataa acattgaaat
     5041 gacaaaatcc cgcgggttgg aagtttaccc gttttccgca gcatttacgt ttgaccataa
     5101 caatgccggg gatttggaaa cggcggcgtt ttattccatt gaaccggatg ataaacaatc
     5161 caaccgggcc attgaaatgg caattaagga tgcattgaaa tccggcggaa tgatacaaac
     5221 ggaattatgg aaggccgtta aggatgcatt gccggatgta accaaaaacc gaatattaga
     5281 ttacatcaaa cgcatggttg ccaaaaatga tttaaatatg gtgcccggca tcaataattc
     5341 ccgtgtttat accttgccgg tttcccggtt ttctacggtt tcccgcggtt ttccggaaaa
     5401 ccgaaccgat aattaaccgg ttttccggtt ttccccccct attaaggggg gaaaccgaaa
     5461 accaaaaacg gcgatgttat tagcggtgta attatgggat ttgatggatt aaaaaataaa
     5521 atccaaatca ttgatttggt ggatggattg aaacgggttg catcttccgg cggcggcgaa
     5581 tttgccgggc catgcccgtt ttgtggggga attgatagat ttagggtgca gccggccaaa
     5641 aacatatggt tatgccggca ttgcacaaac gggatttggc
//
LOCUS       DAOQWE010000049         5537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_14965_length_5537_cov_6.573878, whole genome shotgun sequence.
ACCESSION   DAOQWE010000049 DAOQWE010000000
VERSION     DAOQWE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14965_length_5537_cov_6.573878"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKK37_02450"
     CDS             <1..869
                     /locus_tag="PKK37_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81271.1"
                     /translation="SLSTKGYVANDYSGKNPVVTVAIISENGERRREKTVNLIVKKNS
                     APAIKVRVGSSIASYTSFEVSFEGSTTGDSGNEDGDYIAEIEAWLKDANGKSVGHSRR
                     TMDRKKKLPALSLTPGSVGQYTLTATITDRYGATDTADVTILVTEKGDSGRDKPLLRV
                     MEPINCVAGEFCDLSVRSADKDIFIEYFYQGKKMVKPFKFSPGDHEVLIKAYRLDGNG
                     RRKNAITKTVLVHVADPNASVPVAEPANAAPIAREYLAETQKSPPGIMPLAAGLFAGA
                     LFARRRLSSTER"
     gene            894..1487
                     /locus_tag="PKK37_02455"
     CDS             894..1487
                     /locus_tag="PKK37_02455"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR81272.1"
                     /translation="MEGEYKSVVFSGVPGSGKSTVVKIVAEKTGMPQLSLGDMWRKRW
                     ESSGSDKSFEAYWRDISREDNSAMTEKACQRVLDQAMIGDFRYVYPIHKQRVRKSSVE
                     AGSIVLVFITADIDTRVSRAWNTEKYNNVRSVRELKKVLNNREQDEVRAGRLLFGPEY
                     DYRDGRYYDLIFNSRHICPQHIAQAVMQAMKQPQDYL"
     gene            1580..1651
                     /locus_tag="PKK37_02460"
     tRNA            1580..1651
                     /locus_tag="PKK37_02460"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1612..1614,aa:Asn,seq:gtt)
     gene            1687..2007
                     /locus_tag="PKK37_02465"
     CDS             1687..2007
                     /locus_tag="PKK37_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNR81273.1"
                     /translation="MLKNAKTIWYTYLLLCDKKTFYVGISNNLDERMREHQSKKSFFT
                     KKFYCIDLVYCEGYFTANKAVKREKQIKGWSRAKKQMLITGQLGINACPEIVDSFRDI
                     GCQN"
     gene            2248..2589
                     /locus_tag="PKK37_02470"
     CDS             2248..2589
                     /locus_tag="PKK37_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81274.1"
                     /translation="MKTIKWTARIIALCVLLFALPFYFGYGNPLPFANPDYSLWENVA
                     LAMMPLVFIGLALGWKYPKVGGWLIVAAVATGFAVGFLTEANLSANLAVPLIPGILYL
                     IDGYKNTRKVA"
     gene            2803..2982
                     /locus_tag="PKK37_02475"
     CDS             2803..2982
                     /locus_tag="PKK37_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNR81275.1"
                     /translation="MNRKADERNIRKLTRLGKLSIAVTIPRDLVVELGWREGQKVVAK
                     KRGRGVFIEDWQPKD"
     gene            3055..3531
                     /locus_tag="PKK37_02480"
     CDS             3055..3531
                     /locus_tag="PKK37_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81276.1"
                     /translation="MSNQNEECCPKFDPAPWDGKVLEWGNKKFIKGKVFTLFFVPVNF
                     GSVIVKLMAKVKDAGGGTPENLCLSDHTSRWNMDLYLAVDKEIPRAENVVLSGRFFSK
                     VYEGDFKETGEWCRDFENIAKERGMKIEKWYMWYTTCPKCAKKYGKNYTVIVGKIN"
     gene            3683..4072
                     /locus_tag="PKK37_02485"
     CDS             3683..4072
                     /locus_tag="PKK37_02485"
                     /inference="COORDINATES: protein motif:HMM:NF021463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HNR81277.1"
                     /translation="MTLKQYKICRIAIVFALSMLISVSITLDNYYLPIVFVITAAAAM
                     YYCKKQLNAKEVMADERDYQIAGTASRYAISIYGWIATVGIFILMAISPSRDNALYIA
                     AQCWAFSVCFLLLANAFIFRYLIKKQK"
     gene            4075..4260
                     /locus_tag="PKK37_02490"
     CDS             4075..4260
                     /locus_tag="PKK37_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81278.1"
                     /translation="MKNKIKEMRNESGMTQEELAQKAGVRRETIVFLEQGKYNPSLKL
                     AMEIAKVFKMKVDDLFY"
     gene            4352..5002
                     /locus_tag="PKK37_02495"
     CDS             4352..5002
                     /locus_tag="PKK37_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HNR81279.1"
                     /translation="MKKSIIFALLIVLASFGVSAYFYPQMPETVVSHWDINGEANGYM
                     SKFWGLFLMPIISLAIFLFFLAIPKIDPLKSNIEKFRKYFDAFIVVFLLFFLYVHLLT
                     VVWNLGARFNMVQFMSPAFSVLFYCCGVLLEKAQRNWFIGIRTPWTISNEEVWDKTHR
                     LAGKLFKITGIIALGGLILPDAAFYLVIFPILFFSLYITVYSYWEYKNISRKHKNL"
     gene            4999..5475
                     /locus_tag="PKK37_02500"
     CDS             4999..5475
                     /locus_tag="PKK37_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HNR81280.1"
                     /translation="MKKSIWIIVIIVIFCAGAAAWYVGRKNDAGSSGETPSGQTGLPE
                     QSAAGEQTFIAQAVYACDGGKTITASFYEGEPIFVEPGQMPIPAGNVKIVLDDGRNFD
                     LPQTISASGVRYANEDESFIFWNKGDAVMIFENGEEKNYTNCAAPAAGGDQAEINQ"
BASE COUNT         1502 a         1139 c         1423 g         1473 t
ORIGIN      
        1 ggtcgctttc caccaaagga tatgtggcca atgattattc gggcaagaat cccgtggtaa
       61 cggtagccat tatctccgaa aacggagaaa ggcggcgcga aaaaaccgtt aatctgatag
      121 ttaaaaaaaa ttccgcgccg gccattaaag tcagggtcgg ttcttcaatt gcctcataca
      181 catcttttga ggtttcgttt gaaggatcaa ccactggcga ctccggtaac gaggacggcg
      241 attatatcgc cgaaattgaa gcctggctga aggatgccaa tggtaaatcg gtgggccaca
      301 gccggaggac catggatcgg aaaaaaaaat tgccggcctt gagtttaacg cccggaagtg
      361 tggggcaata tactctgacc gctaccatca ccgaccgcta cggcgcgacc gacaccgccg
      421 atgtcaccat tttggtgacc gagaaaggag atagcggcag agacaagccg ttgctccgtg
      481 tgatggaacc cattaattgt gttgccgggg agttctgcga tcttagtgtc cgcagtgccg
      541 ataaagatat tttcatagaa tatttttatc aagggaaaaa aatggttaag ccatttaaat
      601 tttcccccgg cgatcacgag gttctcatta aggcatatcg ccttgacggg aacggccggc
      661 ggaaaaacgc aatcacgaaa accgtattgg tccatgttgc cgatcctaat gcctctgttc
      721 ccgttgccga acccgcgaac gcggcgccga ttgcgcgcga atatctggcc gagactcaaa
      781 aatcgccgcc gggaattatg ccgctggcgg caggattatt cgccggcgcg ctgtttgctc
      841 gccgccgatt gtcgagtacg gaaagataaa cagaatgaaa ggagttgata tgagtggaag
      901 gagaatataa atccgttgtt tttagcggcg ttcccgggtc cgggaaatca acagtagtga
      961 aaattgtggc tgaaaaaaca ggtatgccgc aattgtcgct gggagatatg tggcgcaagc
     1021 gatgggaatc atctggttcc gataagtcat ttgaggctta ttggagagat atttcccgcg
     1081 aagataattc ggcaatgacc gaaaaggctt gccagagggt gcttgatcaa gccatgatcg
     1141 gagatttccg atatgtatat ccgatccaca aacaacgtgt tagaaagtca tcggttgagg
     1201 ctggttctat agtattagtt tttattactg ccgatatcga taccagggtt tctcgagctt
     1261 ggaatacgga aaaatacaac aatgtaaggt cggtaaggga gctaaagaaa gtcctcaata
     1321 accgtgaaca ggatgaggtc agggcggggc gtttgctctt tgggcctgag tatgactatc
     1381 gggatggaag atattacgac ctcatattca atagccggca tatctgcccc cagcacattg
     1441 cacaggcagt aatgcaggca atgaaacaac ctcaggatta tttataatcc ttttttattt
     1501 atatttgcct ctcttgaaaa aaacctgatg atggggtagt atcaggttgt tgatgacgag
     1561 gagcaaacga agacattatt ccggcgtggc gcaacggtag cgcggttcgc tgttaacgaa
     1621 aaggttgtag gttcgaatcc taccgccgga gcccttcggc aagctcaggg caagccctaa
     1681 aaagccatgc tcaagaatgc caaaactata tggtacacat atttacttct ttgcgacaag
     1741 aaaacttttt acgtagggat atcgaataat ttagatgaaa gaatgcgcga gcatcaaagc
     1801 aagaagtcat ttttcacaaa aaagttttat tgtatcgatt tagtttactg cgaaggatat
     1861 tttaccgcca ataaagccgt gaagagggaa aaacaaataa agggttggag ccgtgcaaag
     1921 aagcaaatgt tgataactgg ccaacttggg attaacgctt gcccggagat tgttgattcg
     1981 ttccgcgata tagggtgcca gaattgagtt tatgctgagc gtagccgaag caaatcctac
     2041 cgccggagcc cagtttcaag aatcgccgat tcggcggttt tttggtggaa tggggcaatg
     2101 aagccgtaaa tcgggtaaaa attaaaattg agattgtggc gtggatttat cttgtaaatt
     2161 tcactataat aaatcaatcg ggagcaggct tcaaagcgtt ccggccgctt cgctgaacgc
     2221 gccgtgtccg gaagtaatat aaaccatatg aaaacaatta aatggaccgc cagaataatt
     2281 gcattgtgcg ttttgctttt cgctctgccg ttttatttcg gctatggcaa cccgttgccg
     2341 tttgccaatc ccgattattc gttgtgggag aatgttgcat tggcgatgat gccgctggtt
     2401 tttatcgggt tggctctggg ctggaaatat cccaaagtcg gaggatggct tattgtcgcc
     2461 gcggtcgcaa cgggttttgc cgtcggattt ctgacggaag cgaatctgag cgccaacttg
     2521 gcggttcctc tcatcccggg aattttatat ttaattgatg gctataaaaa tacccgaaag
     2581 gttgcttgat gaattttgcc ccgcaccggc aacgccggca aaaacgacag gggttgggcc
     2641 cctgttttta acccccgtta tttggttttc catattccat gcggaatatg ggcgccaagc
     2701 cgtgcggtgg ttagaggttg aaaggaaggg cgggggaatg ttatatttat gttatatttg
     2761 tgctatattt aaacatagca tcattgataa ccgcaaaata atatgaatcg caaagctgac
     2821 gaaagaaaca tccgcaagtt gacgcgattg ggcaaattgt caatcgcggt gaccattccg
     2881 cgcgatctgg tcgtggagct tggctggcgc gaaggccaaa aggtcgtggc caaaaagcgc
     2941 gggcggggcg ttttcattga agactggcaa ccaaaggatt gaataattaa tcaaattggt
     3001 tcataaaaag ccaaacatat ttcgtgctga attttgaaag cattgtaaat taatatgagc
     3061 aatcaaaacg aagagtgctg tcccaaattt gatcccgcgc cttgggacgg aaaggttttg
     3121 gagtggggga ataaaaaatt tatcaagggc aaggttttca ctttgttttt tgtgccggtg
     3181 aattttggat cggtgatcgt taaattgatg gccaaggtca aggatgccgg cggcggcacg
     3241 ccggagaatc tttgtttgtc ggaccacact tcaagatgga acatggattt gtatctggcg
     3301 gtggacaaag aaattcccag ggctgaaaat gttgtgctgt cgggcagatt tttcagcaag
     3361 gtctacgaag gggatttcaa ggaaaccggc gagtggtgcc gggattttga gaatatcgcc
     3421 aaagagaggg ggatgaagat tgaaaaatgg tatatgtggt acaccacctg ccccaagtgc
     3481 gccaaaaaat acggcaagaa ttacacggtg attgtcggca agatcaattg aacgcgtctg
     3541 gcggcgcgga ttgaaaatca acggggagcg tccccgtttt tgttttggcc ggcgattcgg
     3601 ggcgattttt ccccggcggg gcttgacata aaagttagaa taatataaca ttataattgt
     3661 tagaaataat taactaaaac gtatgacatt aaaacaatac aagatttgcc gcattgcgat
     3721 tgttttcgct ctttcaatgc ttatcagtgt ttccatcacc ttggataact attatctgcc
     3781 aattgttttt gtgatcaccg ccgccgcggc gatgtattat tgcaagaaac agctcaacgc
     3841 caaggaagtg atggccgacg aacgagatta tcaaatcgcg ggcacggcct cccgctatgc
     3901 tatctcgatt tacggctgga tcgccacggt cgggattttt attttgatgg cgatttcccc
     3961 ctcaagagac aatgcgcttt atattgcggc ccagtgctgg gcgttcagcg tttgctttct
     4021 gcttcttgcc aacgctttca ttttcagata tttgatcaaa aaacaaaaat agcgatgaaa
     4081 aacaagatca aagaaatgag aaacgaatca gggatgactc aggaagaact ggcgcaaaaa
     4141 gccggtgtcc ggcgggaaac gatcgtgttt ttagagcagg gaaaatacaa tccttccctc
     4201 aagctggcga tggaaattgc caaagttttc aaaatgaagg tggacgattt gttttattga
     4261 ggcaattatc tgttataatg ggtttaacgg attgtccggt cgcctcgcgg caaatcatgc
     4321 tccgtggaaa atttttccgc ggttccaaaa tatgaaaaaa agcatcattt tcgcgttatt
     4381 aatcgttctg gcgtcttttg gcgtgagcgc ttatttttat ccgcaaatgc ccgaaactgt
     4441 ggtatcgcat tgggacatta acggtgaagc caatggttat atgtccaaat tttggggatt
     4501 gtttttgatg ccgatcattt ccttggcgat atttttgttt tttttggcca ttccgaaaat
     4561 tgatccgctc aaatcaaaca ttgaaaaatt cagaaaatat tttgatgcgt ttatcgtcgt
     4621 tttccttctc ttttttctct atgtccatct tttaaccgtg gtctggaatc tgggagccag
     4681 attcaatatg gttcagttta tgtcgccggc gtttagcgtg ctgttttatt gctgtggggt
     4741 attattggaa aaggctcaac ggaactggtt tatcggcatc agaactccgt ggacgatcag
     4801 taacgaagaa gtctgggaca aaactcatcg ccttgccgga aaattattca agattaccgg
     4861 cataatcgcc cttggaggat tgattctgcc cgacgcggct ttttatctgg ttattttccc
     4921 gattttgttc ttttcgcttt acatcacggt ctattcttac tgggaataca aaaatatttc
     4981 ccgcaaacat aaaaacctat gaaaaaaagt atttggataa tcgttatcat tgtaattttt
     5041 tgcgcgggcg cggcggcatg gtatgtcgga cgcaaaaatg acgcgggttc ctccggagaa
     5101 actccttcgg gccaaacggg actgccggaa caaagcgcgg ccggcgaaca aactttcatc
     5161 gcgcaagcgg tttatgcttg cgacggcggc aaaaccatta cagcgtcttt ttatgagggc
     5221 gagcccattt tcgttgaacc cggacaaatg ccgattcccg cgggtaacgt gaaaattgtt
     5281 ttggatgacg ggcggaactt tgacttgccg cagacgattt cggcatcggg agtccgctat
     5341 gccaatgagg acgagtcgtt cattttctgg aacaagggtg atgctgtgat gatttttgaa
     5401 aacggcgagg aaaagaacta caccaattgc gccgcgccgg cggccggagg cgatcaagcc
     5461 gaaatcaatc agtagtccgt tgctttgata tccaaacagc tccgttttgg cagtaaaaca
     5521 cccttacaaa cttgagt
//
LOCUS       DAOQWE010000050         5500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_15088_length_5500_cov_8.730395, whole genome shotgun sequence.
ACCESSION   DAOQWE010000050 DAOQWE010000000
VERSION     DAOQWE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15088_length_5500_cov_8.730395"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PKK37_02505"
     CDS             complement(<1..391)
                     /locus_tag="PKK37_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81281.1"
                     /translation="MQTENVFARSLAAVCAAAFLSILSAPAAQAAFPQDQAGIAAYVK
                     LTGVSQTTFDTALDDFFDSTEPSGDTYMIGVKGYLVDDQPYDPYNYQYNKTDIHFYLG
                     VDGWLAAYLLKDDPAAKIVNWRPGAELK"
     gene            complement(500..2350)
                     /locus_tag="PKK37_02510"
     CDS             complement(500..2350)
                     /locus_tag="PKK37_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNR81282.1"
                     /translation="MIIVISFFMGEKEKIIYIGEVNWRHQRTKFGIKTDDRRRHMYLV
                     GKTGMGKSSLLENMAIQDIHAGNGIAFIDPHGEAAEKLLDYVPSWRVNDVIYFNPGDV
                     AYPTAFNVMEITNPEHRNLIAAGLMAVFKKIWPDVWSARMEYILNNTLLALLEYPNST
                     LLGINRMFSDPEYRARVVERVTDPIIKAFWVNEYAKYNQKYEQESTAAIQNKIGQFIA
                     NPLMRNIIGQVKSSIDMRQIMDEKKILIVNISKGMVGEDASRLFGAMVITKLQLAAMS
                     RQNIPEEQRTDFYCYVDEFQNFATDSFVNILSEARKYRLSLILANQYLGQLEEMTGVG
                     KSSRVKDAVFGNVGTIIVFRVGAEDAEFLEPEFTPEITIEDFVNLGKYHIYLKLMIDG
                     ITSAPFSAVTLPPLEMAEPSEKEKITRVSRERYGVRREIIEEKIAKWSGEMSEAAAKV
                     PPMPEAGRRDPDQPKMHEITCSMCGKKTKVIFEPEPGRKVYCKPCLKKLQGEEKRDDS
                     RANGGDRRPPAPTAGTDRSKNALPPSSFALPRPTMPARVSLADLARTEPVRFAGQKND
                     PEKSRPKKEPQIEDLKKALRDALADANSNPTPNSNPNPDPNPDIPAAPPE"
     gene            complement(2347..3663)
                     /locus_tag="PKK37_02515"
     CDS             complement(2347..3663)
                     /locus_tag="PKK37_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81283.1"
                     /translation="MVNEIGQTINLAWRIVLYWWWLPLFFVLVKKFDYYWMWWRTELW
                     FGTIWKPLLLEIKIPKELPKPIKAMEAVMVALHGAIYNGPDWWEKYITGEPQTSLKFE
                     IAGIDGKIHFYVRCFAEYREAIEAAIYSQFPEAEIEAAVDYSRAVPFDIPNRDWDLFG
                     WDYKEAKKPQYPLPTYERYVEPNAEDEEIVDPISSLMESLGKLKPGEQIWIQISASPI
                     YFGTDKANAFIKKSHHERDVLSKRIDEAAGKSRPLWQELLEFIIEGPKAEEPKAPEMF
                     PAELRLTAGERELVEAVEKKASKPLFSCYIRNIYVAQNDARVPSNWRLMLNYFNNFTS
                     AESNSLEVWGKTLTKVKFSPLPFVNDLAPRRAFVKKRRLLRTYRDRLNYYSPWGAGEG
                     DEGAAIVLNVEELASLYHFPSQAVAPSPGITRTESRETVAPSNLPV"
     gene            complement(3737..4783)
                     /locus_tag="PKK37_02520"
     CDS             complement(3737..4783)
                     /locus_tag="PKK37_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNR81284.1"
                     /translation="MANINYKKICNELLEALAPKQREVIERRFGLKTGKRAASGEFGF
                     RRETLEAIGKDYGICRERVRQVEKAAMTKMKKRIAGYREVPQVFGRHLKNAGGVREEA
                     ALLEELGGGEFEPHVNFLLVLGGEFIRFAENDPFKSFWAIDDQSAQAAKNFVSSAARF
                     LEEKKSLAAIRELASSSGLKKSIAESYLGISLRVQKNRDGLYGLREWPEVNPKGIKDK
                     IYLLFRKTGNPLHFTEVARQIEGSLVQTVHNELIRDRRFVLIGRGKYALKDWGYVAGD
                     VKDVILKIFGEENRPLTRREILDRVMKQRQIKENTVLMNLSNKKYFSRDNEGRYWRAA
                     DSEFGSPRANVQEA"
     gene            complement(4856..>5500)
                     /gene="secF"
                     /locus_tag="PKK37_02525"
     CDS             complement(4856..>5500)
                     /gene="secF"
                     /locus_tag="PKK37_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNR81285.1"
                     /translation="LSAQRREALLAALKETGEFEEKSFESIGPMVGRELTGKMSLLVL
                     VSLLAMLLYIAVAFRNVPGQIGAWQFGAASFLILCHDILIPLGIFAALGAFWGVQITI
                     PVITALLIVVGYAINNVIVVYDRVRENLLRDRRADFAENANRAINQTLSRQINTSLAT
                     LLPVFAIYFLGGESLKYFSLALILGVAVGTYSSVFLSAQILVLWRQIKARRQNV"
BASE COUNT         1268 a         1520 c         1333 g         1379 t
ORIGIN      
        1 ttttcaattc cgcgccgggg cgccaattca cgattttggc ggcgggatcg tcttttaaca
       61 gataggccgc cagccagccg tcaacgccca gatagaagtg gatgtcggtt ttgttgtact
      121 gataattata ggggtcatag ggctgatcat caacaagata tcctttgacg ccgatcatat
      181 aggtatcgcc ggacggttcg gtggaatcaa aaaaatcatc caaagcggtg tcaaaggttg
      241 tttggctgac gccggttaat ttcacgtagg cggcaatccc cgcctgatcc tgcggaaagg
      301 ccgcttgcgc ggccggcgcg gataatatac ttaaaaatgc cgcggcgcaa accgccgcca
      361 acgaacgcgc aaaaacattt tctgtttgca tattttttaa tttattttta attatcttga
      421 attataaaac tcttaggcgc cttccgtaaa taacttgccc atattacctt agccaatttt
      481 attggtgaga ttatttttat tattccggcg gggcggcggg aatatccgga ttcgggtccg
      541 gatttggatt tgagttcggg gtcggatttg aattcgcgtc cgcaagggca tcgcggaggg
      601 cttttttgag gtcttcaatt tgcggctctt tcttggggcg actcttttcg ggatcgtttt
      661 tttggccggc aaaacgcacc ggctcggtcc gcgccaaatc cgccagcgac acgcgggcgg
      721 gcattgtcgg ccgcggcaag gcaaaggaag aaggcggaag agcgtttttg gaacggtcgg
      781 tgccggcggt cggtgccggt ggtcggcgat cgccgccgtt ggcacggctg tcatcgcgct
      841 tttcttcgcc ttggagtttt ttcaaacaag gcttgcaata aactttgcgc ccgggctcgg
      901 gctcaaaaat aactttggtt ttcttgccgc acattgagca ggtaatctcg tgcattttgg
      961 gctggtcggg atcgcggcgc cccgcttcgg gcatcggcgg aaccttggcc gcggcctcgc
     1021 tcatctcacc gctccacttg gcaatctttt cttcaatgat ttcgcggcgc acgccgtagc
     1081 gttcgcgcga cacgcgggtg atcttttctt tttccgacgg ttcggccatc tccagcggcg
     1141 gcaaagtcac cgccgaaaac ggcgcactgg tgatgccgtc aatcatcagt ttgagataaa
     1201 tgtgatattt gcccaaattg acgaagtctt caatggtgat ttcgggcgtg aattccggct
     1261 ccaaaaattc cgcgtcctcg gcgcccacgc gaaaaacgat gatcgttccc acattaccga
     1321 acaccgcatc tttcacccgg gagcttttgc cgacgccggt catttcttcc aactgtccca
     1381 gatattgatt ggccaaaatc agcgacaagc gatatttcct ggcttcggaa agaatgttga
     1441 caaacgaatc agtggcaaaa ttctggaact catccacata gcaataaaaa tctgttcttt
     1501 gttcttcggg aatgttctgg cgggacatcg ccgccaattg cagtttggta atcaccatcg
     1561 ccccgaaaag ccgcgacgca tcctcaccga ccatcccctt ggaaatgttg acgatcaaaa
     1621 tcttcttttc gtccattatc tggcgcatat caatgctgga cttcacttgc ccgatgatgt
     1681 ttctcatcaa cggattggcg ataaactggc cgatcttgtt ttgaatcgcc gcggtggatt
     1741 cctgttcgta tttttggttg tatttggcat actcgttcac ccaaaacgct ttgatgatcg
     1801 ggtcggtgac tctttccacc acccgggcgc gatattccgg atcggaaaac atccggttaa
     1861 tgcccaacag agtggaattc ggatattcca acagcgccaa aagcgtgttg tttaaaatat
     1921 actccatccg ggccgaccac acgtcgggcc aaatcttctt gaacaccgcc atcagtccgg
     1981 cggcaatcag gttgcgatgc tcgggattgg tgatctccat cacgttaaac gccgtcgggt
     2041 aagccacatc gccgggattg aaatagatga cgtcgttaac ccgccaggaa ggcacgtaat
     2101 ccaaaagttt ttccgcggct tccccgtgag gatcaataaa cgcaatgccg tttcccgcat
     2161 gaatatcctg gatcgccata ttttccagca ggctggattt gcccataccg gttttcccca
     2221 ccagatacat atggcggcgg cgatcatcgg ttttgatgcc aaatttggtg cgctgatgcc
     2281 gccaattaac ttctccgata taaatgattt tttctttttc tcccataaaa aaggaaatga
     2341 cgattatcaa acgggcagat tggacggcgc cacggtttcg cgcgattccg tgcgagtgat
     2401 gcccggagag ggcgccaccg cctgcgacgg gaaatggtag agcgacgcca gctcttcaac
     2461 attcaaaacg atggccgcgc cttcgtcgcc ttccccggcg ccccacggac tgtaataatt
     2521 caagcgatcg cgataggtgc gcaaaagccg ccgcttttta acaaacgccc gccgcggcgc
     2581 cagatcattg acaaacggca atggagaaaa cttgaccttg gtcagggttt ttccccaaac
     2641 ttccaaagaa tttgattccg cgctggtaaa attattgaaa taattaagca tcagacgcca
     2701 gtttgaagga acccgggcat cgttctgcgc cacgtaaatg ttgcgaatat agcaggagaa
     2761 cagcggcttg gacgcctttt tctccaccgc ctccaccaat tcccgttcgc ccgcggtaag
     2821 gcgaagctcg gccggaaaca tctccggcgc cttcggctct tcggccttgg gcccctcaat
     2881 gatgaattca agcaattcct gccacagcgg ccggctcttc ccggcggctt cgtcaatcct
     2941 ttttgataaa acatccctct cgtgatggga ttttttgatg aatgcgttgg ctttgtcggt
     3001 gccgaaatag atggggctgg cgctgatctg gatccaaatt tgctcgcctg gcttcaattt
     3061 tcccaaactc tccatcaatg acgatatcgg atcaacaatt tcttcatctt cggcgttggg
     3121 ttccacatag cgctcgtagg ttggcagggg atactgcggc ttcttcgcct ctttgtagtc
     3181 ccagccgaaa agatcccaat cccggttggg aatgtcaaac ggaaccgccc gcgaataatc
     3241 caccgccgcc tcaatttcgg cttcgggaaa ttgcgaataa atggccgctt caatcgcctc
     3301 gcggtattcg gcaaaacacc gcacgtagaa atggatcttg ccgtcaatgc cggcaatctc
     3361 aaacttcaag ctggtttgcg gttcgccggt aatgtatttt tcccaccagt cggggccgtt
     3421 gtagatcgcg ccgtgcaagg ccaccatcac cgcctccatc gccttgatcg gcttgggcaa
     3481 ttctttggga attttaattt ccagcaaaag cggcttccag atcgtgccaa accacaattc
     3541 cgtgcgccac cacatccaat aatagtcaaa ttttttgacc agcacaaaaa acagcggcaa
     3601 ccaccaccaa taaaggacga ttctccacgc aagattgatt gtttggccaa tttcgttaac
     3661 cacaattcat caattaaaca aaaacaagcg ccgatgcgct tttattatat attgagataa
     3721 ccgaaattga aaggaattat gcttcctgaa cgttggcgcg cggcgaaccg aactcgctgt
     3781 cggcggcgcg ccaatagcgc ccttcgttgt cccgggagaa gtatttcttg ttgctcaaat
     3841 tcatcagcac cgtgttttcc ttgatctggc gctgtttcat cacgcggtca agaatttcgc
     3901 ggcgcgtgag cgggcggttt tcctccccga agattttcaa tatcacatcc ttgacgtcgc
     3961 cggcaacata tccccaatct ttcagggcgt atttgccgcg gccgatcaaa acaaagcgcc
     4021 ggtcgcgaat cagctcgttg tgcactgttt gcaccaaact gccttcaatt tgccgcgcca
     4081 cttcggtgaa gtgcagcgga ttgccggtct ttctgaaaag caaatatatt ttgtctttga
     4141 tgcctttggg attgacttcc ggccattcgc gcaagccgta gagcccatct ctgtttttct
     4201 gcacccgcag ggatattccc agataggatt cggcgattga ttttttcaat cccgaagacg
     4261 acgccaattc gcgaatcgcc gccagagatt ttttttcttc caaaaaccgc gccgcagacg
     4321 acacgaaatt cttggcggcc tgagccgatt ggtcgtcaat cgcccaaaaa cttttgaaag
     4381 gatcattttc ggcaaaacgg atgaattccc cgcccaagac cagcaaaaaa ttcacgtgcg
     4441 gctcaaactc gccgccgccc aattcttcca acagcgccgc ttcttcgcgc acgccgccgg
     4501 catttttcaa atgccgtccg aaaacctgcg gaacttccct gtagccggcg attctttttt
     4561 tcattttcgt catcgcggcc ttttccacct gccgcacgcg ttcgcggcaa atgccatagt
     4621 ctttaccgat cgcttccaag gtttcccgcc gaaaaccaaa ctcgccgctg gcggcgcgct
     4681 tgccggtttt aagcccaaaa cggcgctcaa tgacttcacg ttgtttggga gcgagcgctt
     4741 ccagcaattc gttgcaaatt tttttgtaat taatgtttgc catggaccgc ggtcaattca
     4801 cagatcagaa ccaatttaac cgattttaat atattacgtg atgttttgat ttttgtcaaa
     4861 cattttgccg gcgcgcctta atttggcgcc aaagaaccag tatttgcgcg gacaaaaaga
     4921 ccgaagagta ggttcccacg gccaccccca aaatcagcgc cagcgaaaaa tatttcaggc
     4981 tttcccctcc caaaaaataa atggcgaaca ccggcaggag tgtggccagc gaagtgttta
     5041 tctggcggct cagagtttgg ttgattgcgc ggttggcgtt ctcggcgaaa tccgcccgcc
     5101 ggtcgcgcag taaattttcg cgcacgcggt catagaccac gatcacgttg ttgatcgcat
     5161 atcccaccac aatcaacagc gcggtgatca ccggaatggt gatttgcacg ccccaaaaag
     5221 ctcccagcgc ggcaaaaatc cccaaaggaa tgagaatgtc gtggcacagg atcaaaaacg
     5281 acgccgcgcc gaactgccac gcgccgatct gtccgggaac gttgcgaaac gccaccgcga
     5341 tatacaaaag catcgccaac aaagaaacca gcaccaacag cgacattttg ccggtgagtt
     5401 ccctgcccac catcggcccg attgattcaa agcttttttc ttcaaattcc ccggtctctt
     5461 ttaacgccgc cagcagggct tcgcgccgct gggcggacaa
//
LOCUS       DAOQWE010000051         5391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_15487_length_5391_cov_6.818778, whole genome shotgun sequence.
ACCESSION   DAOQWE010000051 DAOQWE010000000
VERSION     DAOQWE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15487_length_5391_cov_6.818778"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1251)
                     /locus_tag="PKK37_02530"
     CDS             complement(145..1251)
                     /locus_tag="PKK37_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR81286.1"
                     /translation="MKTKTAIKILVAVSIAAIGGTMFFLNNSSRLPIADEKSELKSES
                     ETREITDGAGKKITIPAKVERIAAAGALNQIVFMLGGGDKLVATAEGVQKGFFPVVFP
                     RIKEIPAAYGGPGPGTLNMETLLKANPQVVFGSYVNDKDVETLASAGIALFELKLNTP
                     PDIKDTISMVGKIIGPEAEQKAADFNRDYDQNLDYARQLTAGALKVKVFAASSDGGGG
                     PISTVPGNDILTSYIDAAGGVNIAAEKLPTALADGSASVDFEFLIAQQPEVIIAQNRQ
                     IYDYITDEKNGSQWQYLDAVKNKKVYLNPKGVYLWSVRSAEGALEPLWLAKILHPEPV
                     ASLDIKQKTKEFYRDNYYYELSDAQVENILFPEN"
     gene            complement(1322..2485)
                     /locus_tag="PKK37_02535"
     CDS             complement(1322..2485)
                     /locus_tag="PKK37_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR81287.1"
                     /translation="MKIPVPNRRAVKPLAFASFLNDFGSDIVYAVWPIFVISVSGANM
                     AILGLIDGLGDAVVSISKAISGYWSDRIKKRKVFVWTGYFLGALSRIGYAFAPVWHWL
                     VPLKVLDRAGKIRSAPRDAIVADESDFSNRGKNFGILKAADHIGAFAGIIFAIGLFPL
                     LGYQKLFLFAAIPSLIGAIFIVAFIKEKKNAEKKIFPGLRFRDLDANFRLFMIASAVF
                     SLGAFSYSFLMIFANKYGFAETTIPLLYLIFTAVAAAVSIPFGRLSDKIGSRKKVFLL
                     SYILWIAVCALFMANQSAAAIIAGFILYGLHKGAFEAVQKALVSELAPEKYRASSLGA
                     IQLVTGLCALPASLAAGIMWSAFNPFVPFFISITLASVSGILMVFVKEKIRQN"
     gene            complement(2503..3306)
                     /locus_tag="PKK37_02540"
     CDS             complement(2503..3306)
                     /locus_tag="PKK37_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNR81288.1"
                     /translation="METEDKYIVAGISLVLLAVILVLFSPFAIVGAGEKGVVLNWGAV
                     SDRVLGEGLHLIVPVYQSVKKINAKTIKYETDVAAYSSDIQNLETKIALNYHLRGDRV
                     NKIYQEIGDIGAVEDTIINPLIRETVKAAIAKSPAQELIDKRTVLLEEIRANLAEKLE
                     KRGIVFDDVSMVDFRFSDQYEKAVELKQVAQQQALKAENDLKRIKTEAEQRVTQAQAE
                     AEAIKIQTAAITQQGGKDYVQLKAIEQWDGALPKSMVSNATLPFLNIEQ"
     gene            3527..3826
                     /locus_tag="PKK37_02545"
     CDS             3527..3826
                     /locus_tag="PKK37_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNR81289.1"
                     /translation="MNKKLYVGGLPYSTTQDGLQQGFSAAGNVESAIVITDKMSGRSK
                     GFGFVEFATEEEAAKAIEMFNGQEFEGRRITVSEARPMEPRAPRRDFNDNGYGDR"
     gene            3920..4606
                     /locus_tag="PKK37_02550"
     CDS             3920..4606
                     /locus_tag="PKK37_02550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR81290.1"
                     /translation="MNKQITIILLGAAVVAAITFAVMSSQKNQKETPKTEINNNQEMK
                     ESASAGVGNAENMSSAAPALDPEYQKIMNATIKTSLGEIKVELAGDKTPITAGNFAKL
                     AENGFYDGVKFHRVISGFMIQGGDPLTKDDARKESWGTGGPGYKFNDEPFEGEYSRGT
                     IAMANSGHNTNGSQFFIMHQDYPLPPSYVIFGKVLEGMEVVDAIAALKTDASDRPLVP
                     PVIESIIVNR"
     gene            4799..>5391
                     /locus_tag="PKK37_02555"
     CDS             4799..>5391
                     /locus_tag="PKK37_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-specific 2-thiouridylase"
                     /protein_id="HNR81291.1"
                     /translation="MDKANDKIKTEGTATATVAAAVSGGVDSAVSAGMLKNGGFEVVG
                     VFARLSGNKTEDEKQARAVCKKLEIPFRAVDLRAEFKKRIIDKFTANTEKGLTPNPCV
                     FCNEEIKFGLLMEAALKSGADYFATGHYCQNKIDKSGIYHLSKGADKKKDQSYFLWRL
                     KQKQLGKIIFPLGVFKKNEVKELAKIWQLPVKNNSES"
BASE COUNT         1381 a         1248 c         1368 g         1394 t
ORIGIN      
        1 gcggaattcc gtatctgccg atgcacagcg aaaggaaaaa caataaaacc agcgccgcgg
       61 ccaaaacggc gacgatcata ccgtttgaca tttttttcct ccgcggtgcg ggaatttttt
      121 gggaaacttt ggtggtcatg gcggttaatt ctcggggaat aaaatgttct caacttgcgc
      181 atcgctcaat tcgtaataat aattatcgcg gtaaaattct tttgtttttt gttttatatc
      241 caggctcgct accggttccg ggtgcagaat ttttgccagc cacaacggtt ccagcgcgcc
      301 ttccgcactg cgcaccgacc agagataaac gcctttcgga ttgagataaa cctttttgtt
      361 ttttaccgcg tcaagatatt gccattgact gccatttttt tcgtcggtga tataatcgta
      421 tatttgccgg ttttgggcga tgatgacttc gggctgttgg gcgatcagaa attcaaaatc
      481 cactgaagcg gatccgtcgg ccagcgcggt tggtaatttt tcggcggcga tattgactcc
      541 gccggccgcg tcaatgtagg aagtgagaat gtcgttgccg ggaaccgtgc ttatcgggcc
      601 gccgccgcca tcactgcttg cggcgaatac cttgactttc aatgcgcccg cggttaattg
      661 acgggcataa tccaaatttt ggtcgtaatc gcgattgaaa tcggcggctt tttgctccgc
      721 ttccgggccg atgattttgc cgaccattga tatggtgtct ttgatatccg gcggcgtatt
      781 caatttcaat tcaaaaagcg cgattccggc cgaagccaaa gtttcaacat ctttgtcgtt
      841 gacataactt ccgaaaacca cctgcggatt tgctttcagc aaggtttcca tgttcagcgt
      901 cccgggtccg gggccgccgt aggccgcggg aatttctttt atccgcggga aaacgaccgg
      961 aaaaaatccc ttttgcactc cttccgccgt ggccaccagt ttgtcgccgc cacccagcat
     1021 aaaaacgatt tggttcaacg cgccggcggc ggcaatccgc tccactttgg ccgggatggt
     1081 gatttttttg ccggcgccat cggtgatttc ccgcgtttcg ctttcggatt tcaattcact
     1141 tttctcgtcc gctattggca gtcgggagct attgtttaaa aaaaacatcg tgccgccaat
     1201 cgcggcaata gagactgcaa ccaagatctt tatcgcggtt tttgttttca tatttctgct
     1261 ggcgcgataa aaagtatgta tttaaatatg tatttaaatg catactttca aaacgcggtt
     1321 tttaattttg ccggattttt tcttttacga aaaccatcaa aattcccgaa actgaagcca
     1381 gcgttatgga gatgaaaaat ggaacgaaag gattgaacgc gctccacata atgccggccg
     1441 ccagagaagc ggggagagcg caaagaccgg tgaccagctg gattgctccc aggcttgatg
     1501 cccgatattt ttcgggagcc aattcggaaa caagcgcttt ctgaacggcc tcaaacgctc
     1561 ccttgtgaag tccgtagagg ataaatccgg caatgatcgc ggcggcggat tgatttgcca
     1621 taaacaaagc gcaaacggca atccacaaaa tataggagag taaaaaaact ttctttcggc
     1681 ttccaatctt atcggataat cggccgaaag ggattgaaac ggcggccgcg acggcggtga
     1741 aaatcaagta taataaagga atcgttgttt ccgcaaagcc gtatttgttc gcgaaaatca
     1801 tcaaaaaaga ataggaaaac gcgcccagcg aaaacaccgc cgatgcgatc ataaacaaac
     1861 ggaaatttgc gtccaaatcc ctgaagcgca gtccgggaaa aattttcttt tccgcgtttt
     1921 ttttctcctt gatgaaggca acgatgaaaa ttgcgccgat cagcgagggg atggcggcaa
     1981 acaaaaacaa tttttggtaa cccaaaagag gaaagaggcc gatggcaaaa ataattcccg
     2041 cgaacgcgcc gatgtgatca gcggccttga ggatgccgaa attctttccc cggttggaaa
     2101 aatccgattc gtcggcgaca atcgcgtcgc ggggagcgct gcgaattttg ccggcgcgat
     2161 caagaacttt cagcggcacc agccaatgcc ataccggagc gaatgcgtag ccgatgcgcg
     2221 acagcgcgcc caaaaaatat ccggtccaaa caaaaacctt gcgtttttta attctgtcgg
     2281 accaataacc ggaaatcgcc ttggaaattg aaacaaccgc atcgcccaat ccgtctatca
     2341 atcccagtat cgccatattc gcccccgaaa cggaaatcac aaaaatcggc cagaccgcgt
     2401 agacgatgtc ggagccaaaa tcatttaaga aagaagcgaa cgccagcggc tttacggcgc
     2461 gccggttcgg cacgggaatt ttcattggaa tttttttgtc catcactgtt caatgttcaa
     2521 aaacggcaag gtggcgttgg acaccattga tttgggcagg gcgccgtccc attgttcaat
     2581 cgccttcaat tgcacgtaat ctttaccgcc ctgctgggtg atggccgcgg tctggatttt
     2641 gattgcctcg gcctcggctt gcgcttgcgt gacccgctgt tcggcttcgg tttttattct
     2701 tttcagatcg ttttcggctt tcagcgcttg ttgctgggcg acttgcttca gttccaccgc
     2761 cttctcgtat tggtcgctga agcggaaatc aaccatggaa acatcgtcaa aaacaatacc
     2821 tcttttttcc agcttttccg ccaaattggc gcggatctct tcaagcagga cggttctttt
     2881 gtcaatcaat tcttgagccg gcgatttggc aatcgcggcc ttcaccgttt cccggatcag
     2941 gggattgatg atcgtgtctt ccactgcgcc gatatcgcca atttcttgat aaatcttgtt
     3001 gactctgtcg ccgcgcagat ggtaattcag ggcgattttt gtttccaaat tctgtatatc
     3061 gctggaatag gcagccacgt cggtttcata ttttatcgtt ttggcgttga tttttttgac
     3121 cgattggtag acggggacga tcaggtgcaa tccttcgccc agcacccgat cggagaccgc
     3181 gccccaatta agaaccacgc ccttttcgcc ggcgccgaca atggcaaagg ggctgaagag
     3241 aacgagaatt accgccagca gaaccagcga tattccggcg acaatgtatt tgtcttcggt
     3301 ttccatttta tttgtttatt gattattttt gtccacattt tgattatacc gcgttttagt
     3361 ttttaatggc aagcattatt gcgggaatac cgcctttgtg ttgatttttt ttaaattggt
     3421 gatatagtaa aggcataagc gtaggtgttt ctccaaggtt ttcttaaatt aaaattttaa
     3481 gtaccggcga gcgggtcgcc ctaccaagtt aatttttcgc attcaaatga acaagaagtt
     3541 atatgtgggc ggattgcctt acagcaccac ccaagacggc ttgcaacagg ggttttcggc
     3601 cgccggcaac gttgaatcgg cgatcgtgat caccgacaag atgtccggcc gctcaaaggg
     3661 tttcggattc gtggaatttg ccactgaaga agaagccgcc aaggccattg aaatgttcaa
     3721 cggccaggaa tttgaaggcc gccgcatcac cgtcagcgaa gctcgcccga tggaaccgcg
     3781 cgcgcctcgc cgcgatttca acgacaacgg ttacggcgat cgctagtctt tgcaagcaaa
     3841 aatctccccg caaggggagt tttgttttcc gcgggcaatg tggtagaatg attttcgtcg
     3901 atgataaatt taaaaaaaga tgaataagca aatcacgatt attttattgg gagcggccgt
     3961 ggttgccgcg attaccttcg cggttatgag cagtcaaaaa aatcaaaaag aaacgccgaa
     4021 aacggaaata aataataatc aagaaatgaa ggagagcgct tccgccgggg ttggtaatgc
     4081 cgaaaatatg agcagtgccg cgccggcgct tgatcctgaa tatcaaaaaa ttatgaatgc
     4141 caccatcaaa acttcattgg gagaaataaa agttgaactt gccggcgaca aaacgcccat
     4201 caccgccggc aattttgcca agttggcgga aaatggattc tatgacggcg tgaaatttca
     4261 tcgggtgatt tcgggattta tgatccaagg cggcgatccc cttaccaagg atgacgcgcg
     4321 aaaagagtcg tggggcactg gcgggccggg gtataaattc aatgacgagc cgtttgaggg
     4381 agagtattcg cgcggcacaa tcgccatggc caattccggt cacaacacca acgggagcca
     4441 gtttttcatt atgcaccaag attatccttt gccgccgtca tacgtgattt ttggcaaagt
     4501 tttggagggt atggaagttg ttgatgcaat tgccgcgctc aaaaccgatg ccagcgaccg
     4561 cccgctggtc ccgccggtga ttgaaagcat catcgttaat cgttaatctt caatatatcc
     4621 gtatttgtag gggcgggcat tgtgcccgtc ctttttgcgt ttgaattgta ggggcaaacc
     4681 cttgttggtt gccctttgag tgcgggcggc cataaaagcc gcccctacga atgtgcgggc
     4741 ttctgaattt ttatgattgg ctatggattt ttgggcgaga agtggtagat ttaaggagat
     4801 ggataaggca aatgacaaaa taaagacgga aggcacagca acggcgacag tggcggcggc
     4861 ggtatcgggc ggggtggata gcgcggtatc cgcgggaatg ttgaagaacg gcggctttga
     4921 ggttgtcggt gtttttgcgc ggctgtcggg gaataaaacc gaagatgaaa aacaggcgcg
     4981 ggcggtttgt aaaaaattgg agattccttt tcgtgccgtt gatttgcgcg cggagtttaa
     5041 aaaaagaatc attgacaaat tcaccgccaa tactgaaaag gggctgacgc ccaatccgtg
     5101 cgttttttgc aatgaggaaa tcaagttcgg actgttaatg gaggcggcgc tgaaatccgg
     5161 cgcggattat tttgccacgg gtcattattg ccaaaacaaa attgacaaaa gcggaattta
     5221 tcatctgtca aaaggcgcgg acaaaaaaaa ggatcagtct tattttttgt ggcggttgaa
     5281 acaaaaacaa ctggggaaaa tcatttttcc gttaggagtt tttaaaaaaa atgaagtcaa
     5341 agaattggca aaaatctggc aattgccggt aaaaaacaat tcggagtcgc a
//
LOCUS       DAOQWE010000052         5169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_16353_length_5169_cov_7.229566, whole genome shotgun sequence.
ACCESSION   DAOQWE010000052 DAOQWE010000000
VERSION     DAOQWE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16353_length_5169_cov_7.229566"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PKK37_02560"
     CDS             <1..908
                     /locus_tag="PKK37_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNR81292.1"
                     /translation="PLDVRASVSPTYYGENAVLRLLAEQAEAFTLESLGFSAVNAQKL
                     RAAICKPYGMILCTGPTGSGKTTTLYALLKSLNTPEVSIITVEDPVEYAIPGICQIQT
                     NPRAGLTFASGLRGLLRQDPDIIMVGEIRDAETAGIAINAALTGHLLLSTLHTNDAAA
                     ALPRLLDMKAEGYLIASTVNVVIAQRLVRKICPHCKIQKTLGDADLAGLRAALRKNIA
                     ARDFFFGAGCDQCSGTGYAGRTSINEVLILTPEIREAILRRESPKRLKQIAANQGMLP
                     MAGDGLAKAFAGVTSIEEVLRVFYE"
     gene            901..2049
                     /locus_tag="PKK37_02565"
     CDS             901..2049
                     /locus_tag="PKK37_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR81293.1"
                     /translation="MNRLIDSIKKFPLAAFPKKRVFNGEKISTATKAFWIRLWFSESE
                     RIVFIKKLAIMAKAGISINSAIKMMEKNVSRQEVKNLVAGLRLAVEQGKPLARAMKDA
                     GVFGDLAVNVVHIGESSGSLAQNLDYLSGEMQKRRELKRSVAAALIYPVFILIATVGI
                     VIFLSVYIFPKILPVFSGLRGDLPWSTNLLIFFAALMRDYWWLAGIIFLAAVLVWRLL
                     IRTEKAKIFFDYWLLRLPMLGAIFKNYHMANSCRALCLLLRGGGGIVASLELAGKSSG
                     NLAYCRAFGKSALAARSGGSIGENMAEEKIFPSLAAQIICAGEMSGSLEDSLICVARM
                     CEDEMNGAAKNLSAAIEPVLMVFAGLLVGFVATAIITPIYGITQLLQK"
     gene            2021..2518
                     /locus_tag="PKK37_02570"
     CDS             2021..2518
                     /locus_tag="PKK37_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81294.1"
                     /translation="MGSPNCCKNNFRTGISSVECLVVAALLAVCVALSAPLDTGGARF
                     WMFAADRDAALAALRHARARAVAGVCFGESCAGGAAHGVHFDLQNARLIIFQGSDFAS
                     RNKNYDEAVDFENRSVRAVLSSGQDAVFETLSGNTAGFDLVLQDDFGHRADILIGKSG
                     LIDWR"
     gene            2505..3983
                     /locus_tag="PKK37_02575"
     CDS             2505..3983
                     /locus_tag="PKK37_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81295.1"
                     /translation="MIGGNFNGDMFFVNDKKTQSGFLTMEILLAMTVAVFALAGAVLV
                     AFGGQSLDIGTAQNSEALVLAKEMIADRQAAAAGDFRLVNSAAAQTGIFTQKTEVSTH
                     SFFTKKIIASVSWDFGGRRQNVTLQTLISDWRGTAGNDTCDSNLSGDWKNPQVEHFDF
                     AVLSETASSTISGIDAYKNKLYVAVEDTSINTDPTLFIFNILDSKNLSLYQHSGNDIG
                     KANALEADDDFIYLATKSSVNQLQIADRNGAFVSALELKDIDGDDCKKTGSSIFYKDN
                     YVYLGLSEDDVCPELNIINVQNRDSPIIAGRFEVGAKINGIYVKGNYAYLATADSNRE
                     LIIIDITNPSAPTFVADYNLLPDRTKWGYGKTVKIIGDKIYFGRTYIDNSPEFYLFNH
                     KESSVDLLGSYSDNGDPFSVNDIIIRDNLAFILAGSASQGGDLRILDIADPGNIVEEK
                     IVGLPGEAGGRGGVAMDCEGNYLYVASVDGQGKSYLSVITAK"
     gene            3983..4387
                     /locus_tag="PKK37_02580"
     CDS             3983..4387
                     /locus_tag="PKK37_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81296.1"
                     /translation="MEKQTAAQKNRGFVLMEFLIYLFLFGLIFAGLFGGIFAVMETMG
                     KNDARVMARHEGEFLLAKIERERDIASFGAAGGALTLAGNRLNNSQTFVSDFEINLLS
                     ENTARASFVLTAEDRAGREFSQKFMTIYRLPQ"
     gene            4384..4764
                     /locus_tag="PKK37_02585"
     CDS             4384..4764
                     /locus_tag="PKK37_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81297.1"
                     /translation="MKKNFSKNQAGYVALVSAILVSGVMMALAVSVSRAAFWGRFDAL
                     ARENKILAQEIAKGCADQALLKIAKNEEPVVWEMCSDLQINADNDPVYEIIARADWKN
                     SFAQLRIVADRTGGKIKILQWEES"
     gene            4806..>5169
                     /locus_tag="PKK37_02590"
     CDS             4806..>5169
                     /locus_tag="PKK37_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNR81298.1"
                     /translation="MTSKIKKRSEDQNQSGFTLIEILVVIGLIAFLAAVVIIAINPAR
                     QFAQGRNSQRVSNVNAILNAIGQRIADNKGLFRISEDAICTIDLPTATSTTQIIGNAG
                     VNLRPCLVPTYISELPADP"
BASE COUNT         1277 a         1128 c         1421 g         1343 t
ORIGIN      
        1 ggccgttgga tgttcgcgct tcggtgtcgc cgacatatta cggcgaaaac gcggttctgc
       61 gtttgctggc cgaacaggcg gaagcgttta cattggaaag cttgggattt tccgcggtca
      121 atgcccaaaa attgcgcgcg gcgatttgca aaccctacgg gatgattttg tgcacgggcc
      181 ccaccggatc gggcaagacc accactcttt acgccttgct caaaagcttg aacaccccgg
      241 aagtttccat tatcacggtg gaagatccgg tggaatacgc gattcccgga atttgccaga
      301 ttcagacaaa tccgcgggcg gggctgacgt ttgcttccgg attgcgcggg cttttgcgcc
      361 aggatcccga cattatcatg gtgggagaga tccgcgacgc ggaaaccgcg gggattgcaa
      421 tcaacgccgc gcttacagga catcttttgc tttccacttt gcacaccaac gatgcggccg
      481 cggctttgcc gcggcttttg gatatgaagg ccgaaggcta tctgatcgct tccacggtca
      541 atgtcgtgat cgcgcaaagg ctggtgcgga aaatttgccc gcattgcaaa atccaaaaaa
      601 cgctcggcga tgccgatctt gccggccttc gggcggcatt gcgaaaaaac attgccgccc
      661 gcgatttctt ttttggcgcc ggatgcgatc aatgttccgg cacgggatac gcgggacgca
      721 caagtatcaa tgaagttttg attttgaccc cagaaatcag ggaagcgatt ttgcgccgcg
      781 aatcgcccaa aagattgaaa cagattgccg ccaatcaggg aatgttgccg atggccggcg
      841 acggacttgc gaaggctttt gccggtgtta cgtccattga agaagtcctg cgcgtgtttt
      901 atgaatagat taattgattc tattaaaaaa ttccctttgg ccgcatttcc gaaaaagcgg
      961 gtttttaacg gcgaaaaaat ttcaaccgcg acgaaagctt tttggattcg gctttggttt
     1021 tcagaatcgg agcgtatcgt tttcatcaaa aaactggcaa taatggcgaa agccggaatt
     1081 tcaatcaaca gtgccataaa aatgatggaa aaaaacgtgt cccggcagga agttaaaaac
     1141 ttagtcgccg gtttgcgctt ggcggttgaa caagggaagc cgctggcgcg ggcaatgaaa
     1201 gacgccggcg tgttcggcga tctggcggtt aacgtcgtgc acatcgggga atcaagcggc
     1261 agtttggcgc aaaatctgga ttatctgtcc ggcgaaatgc aaaaaaggcg cgaattgaaa
     1321 agaagcgttg ccgccgcgct gatctatccg gtctttattt tgattgccac tgtcggaatt
     1381 gtcatttttc tttccgttta catctttccc aaaattctgc ccgttttttc cggtttaagg
     1441 ggcgatctgc cgtggagcac gaatttgtta atttttttcg ccgccttaat gcgtgattat
     1501 tggtggcttg ccggaattat ttttttggcg gcggttttgg tttggcgctt actgatcaga
     1561 acggaaaaag cgaagatttt ctttgattac tggctgttgc gcctgccgat gctgggcgcg
     1621 attttcaaaa attatcatat ggcgaattcc tgccgcgccc tttgtttgct tttgcgcggc
     1681 ggcgggggaa ttgtggcgtc cttggaattg gcgggaaaat catcgggcaa tttggcttat
     1741 tgccgcgcgt tcggaaaaag cgctttggcg gcccgttccg gaggatcaat cggcgagaat
     1801 atggccgaag aaaaaatttt cccttcactg gcggcgcaaa ttatatgcgc tggagagatg
     1861 tcggggtcgc tggaagattc cctgatctgc gtggcgcgaa tgtgtgaaga cgaaatgaac
     1921 ggcgcggcga aaaatttgtc ggcggccatt gagccggttt tgatggtttt tgcggggctg
     1981 ctggtcggct ttgtcgcgac tgcgattatc actcccatat atgggatcac ccaattgttg
     2041 caaaaataat tttcggacgg gaatttcatc agttgaatgc ttggttgtcg ctgcgttgct
     2101 ggcggtttgc gttgcgttgt cggcgccgct ggataccggc ggagcgcggt tttggatgtt
     2161 cgccgccgac cgcgatgcgg cgctggctgc tttgcgccat gcccgcgccc gggcggtcgc
     2221 gggagtttgc tttggcgaat cctgcgccgg cggcgcggcg cacggagttc attttgattt
     2281 gcaaaacgcg aggttgatta tttttcaggg gagtgatttt gcaagccgga acaaaaatta
     2341 tgacgaagcg gttgattttg aaaatcggtc ggtgcgggcg gttttgtctt ccggccaaga
     2401 cgcggttttt gaaacgcttt cgggaaatac cgcgggtttt gatctggttt tacaagacga
     2461 cttcgggcat cgcgcggata ttttaatcgg caaatccggc ctcattgatt ggcggtaatt
     2521 tcaatggcga tatgtttttc gtcaacgata aaaaaactca atccggcttc ttgacgatgg
     2581 aaattttgct ggcaatgacg gtggcggttt tcgcgctggc cggcgcggtg ctggttgctt
     2641 tcggcggcca atcgctggac atcggcaccg cccaaaattc cgaagcgctg gttttggcaa
     2701 aagaaatgat tgcggaccgg caagcggccg ccgcgggcga tttccgactg gttaattccg
     2761 ccgccgcgca aacgggaatt tttacccaaa agacggaagt gtcaacgcat tcttttttta
     2821 ccaaaaaaat tatcgcttcg gtgagctggg attttggcgg ccgccggcaa aacgtcaccc
     2881 tgcaaacctt gatttccgac tggcgcggca ccgccggcaa cgacacttgc gattcaaatt
     2941 tatccggcga ctggaaaaac ccgcaagtgg aacattttga ctttgctgtt ttatcggaaa
     3001 ccgcttccag tacgataagc ggcattgatg cttacaaaaa caaactctac gtggcggtag
     3061 aagatacttc aataaacacc gacccgacac tgtttatttt caatattctt gattccaaaa
     3121 atctatcgtt gtatcaacat agcggcaacg atattggaaa agcaaacgcc cttgaggcag
     3181 acgatgattt tatttatctg gcgaccaaaa gctccgtcaa ccaattgcaa attgccgata
     3241 gaaacggagc atttgtttct gcgttggagc ttaaagatat tgacggcgat gattgcaaaa
     3301 aaacgggtag cagtattttt tataaagaca actatgttta tttaggatta agcgaagatg
     3361 atgtttgtcc ggaattaaat attattaatg tgcagaatcg tgatagtccg ataatcgccg
     3421 gaagatttga agttggcgcc aagattaacg gtatttatgt gaaaggcaac tatgcgtatc
     3481 tggcaaccgc cgacagtaat cgcgaattaa ttattattga tatcaccaac ccatctgctc
     3541 cgacttttgt cgccgattat aacttgcttc ccgatcggac aaaatgggga tacgggaaaa
     3601 ccgtcaaaat aattggtgat aagatttatt ttggcaggac atatattgat aattcgccag
     3661 agttttattt gtttaaccat aaagaatcat cggtggattt attgggcagt tattcggata
     3721 atggcgatcc attcagtgtt aacgatataa tcatcagaga taatctggcc tttattttgg
     3781 cgggaagcgc cagtcaagga ggggatttga ggatattgga tattgccgat ccgggcaata
     3841 ttgttgaaga aaaaatcgtt ggtttgcccg gagaggccgg cggccgcggc ggggtggcga
     3901 tggattgcga aggaaattat ttgtatgtcg catcggttga cggccagggc aaaagttatt
     3961 tatcggtaat caccgccaaa tgatggaaaa gcaaactgcg gcccaaaaga acaggggatt
     4021 tgttttgatg gaatttttga tctatctgtt tcttttcgga ttgatttttg ccggtttgtt
     4081 cggcggaatt ttcgcggtga tggaaacaat gggaaaaaac gacgcgcgcg tgatggcgcg
     4141 ccacgagggg gaatttttgc tggcaaaaat tgaacgggaa agggacattg cgtcctttgg
     4201 cgcggccggc ggcgcgttga cgcttgccgg aaacaggctc aacaattcgc aaacattcgt
     4261 cagtgatttt gaaattaatt tactttcgga gaatacggcg cgggcaagtt ttgttttaac
     4321 cgccgaggac cgtgccgggc gggaattttc ccaaaaattt atgacaattt atcgtttgcc
     4381 gcaatgaaga aaaatttttc caaaaatcaa gccggatatg tcgcgctggt ttccgcgatt
     4441 ctggtttcgg gagtgatgat ggcgctggcg gtgtcggttt cgcgcgcggc gttttggggc
     4501 agatttgacg cgctcgcccg cgaaaacaaa atccttgcgc aggaaatagc aaaaggatgc
     4561 gcagatcaag cgcttttgaa aatcgctaaa aatgaagagc cggttgtttg ggaaatgtgt
     4621 tccgatctgc aaatcaacgc ggataacgat ccggtttatg aaattatcgc ccgcgccgat
     4681 tggaaaaact catttgccca attgcggatt gtcgccgatc gcaccggcgg gaaaataaaa
     4741 attcttcaat gggaggaatc ctaaaggtcg aaaagtcaag aataaaaaat aaaagtcaaa
     4801 atcaaatgac aagcaaaatc aaaaaacgga gcgaagatca aaaccaatca gggttcaccc
     4861 tgattgaaat actggtcgtt atcggattga tcgcgttttt ggcggcagtg gtgattattg
     4921 ccattaaccc ggcgcggcag ttcgcgcaag ggcgcaattc ccagcgcgtg agcaatgtca
     4981 acgcgatctt aaatgccatc ggccaaagga ttgcggacaa caaaggtttg ttcaggattt
     5041 cggaggatgc aatatgcaca attgacttgc cgacggcgac atcaacaacg cagattatcg
     5101 gcaatgccgg cgtaaatttg aggccgtgtc ttgtgccgac ttacatttcc gaattgccgg
     5161 cggacccgg
//
LOCUS       DAOQWE010000053         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_16645_length_5097_cov_7.601944, whole genome shotgun sequence.
ACCESSION   DAOQWE010000053 DAOQWE010000000
VERSION     DAOQWE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16645_length_5097_cov_7.601944"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKK37_02595"
     CDS             complement(<1..210)
                     /locus_tag="PKK37_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81299.1"
                     /translation="MSNLTREFIIGDIYHLFNRGVEKRQIFMDESDRFRFVFCLYECN
                     DANFVRMDRRIYDRRIRNMARFHLDQ"
     gene            complement(389..2467)
                     /locus_tag="PKK37_02600"
     CDS             complement(389..2467)
                     /locus_tag="PKK37_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNR81300.1"
                     /translation="MNSKKLFAGAVALAMAAAIVPSAGQAATAEELAAQIAQLQAQLA
                     SLMGQLGGTTTTTGTTSSTAPAACAGISFTRNLTLGSQGTDVQCLQALLNQDPATQVA
                     ASGVGSAGNESQYFGGLTKAAVVAFQNKYATEILAPVGLTAGTGFVGASTRAKLNAML
                     VGGTTGTTGETGTTGTTGTTLPEGCTSTAGYSPVTGESCSSTSTSTGTGLTMTGQEGS
                     ITVSINPSPANGTKTYEGDEKVAVMGLKVKATGSDVDVQRVTLQFGAQPYSYFTNVYL
                     YDGDTQVASSALNSTTVSKVTSTNYQITLAGFTSKVIVPVNTDKVLTVKLDVLPGISS
                     GLFQTTNTSQTINIGMPSATAVRAVDQAGLNQYGGVEYGDVNRSFTVNESQAANATLA
                     LSLNANSPKKRNIVADSNQEISGATLMTFDLKASKDDLIIDEILDVDFGASQIPATAY
                     VVDDGGTVIGTISPSATAEKNDFTELDYTISKDTTKTLSIKVDDTLAAPNTGTRVSAD
                     DGDTYTVAIGTGKVGFTKSNGTTGTSTGTATSNAAYIYAEGPEFTLASITTDNTQAPF
                     DGASSTMSATFNIQVKAVNGDVYISATSTDAFVIKSSVGGVQATLNDVTYVQPDNTVL
                     VGGTYKVSEGNSATFAVKATASNGGVAGTYDIRVNSINWGHLSTTPTSVTSSYMNGEA
                     AWISPAIYLR"
     assembly_gap    2620..2719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2720..3453)
                     /locus_tag="PKK37_02605"
     CDS             complement(<2720..3453)
                     /locus_tag="PKK37_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81301.1"
                     /translation="MNSEKALINRIRTLREIAPDNDWVVSCKTRIMGPEAMAAKSSPM
                     IFLRRFVFQYRAALAGLILAAGAGGGLLVSAQNALPGEPLYGLKKATEKGSAMVMGQV
                     RRGESAAANLRLAAKRLEEIDIISRRNLAQNLPAAMDEYQAAKTEAKKEVAALVKKNP
                     QKAGEIAKQAARAMKEINDREKQVYTVLGAEPGAGEEDLRAEVVLDKQIIESLILYLN
                     AEEMLSQEEIVDLNEVRKLYSAGDYS"
     gene            complement(3559..4062)
                     /locus_tag="PKK37_02610"
     CDS             complement(3559..4062)
                     /locus_tag="PKK37_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNR81302.1"
                     /translation="MDKNQEKFSRLYDDCAKKVYRFVFLRVNSKEVAQDLTAEAFARC
                     WEKFKKDPGAVQNPRAFLYKTARNLMVDHYRAAGKIRTVPPETVENYLPDPKIDLEKK
                     AMLASEMNTVRQAMSHLNDDYQNAIIWRYLDDLSIKEVSQLLDRSEEATRVLLHRAMK
                     ELKTYLS"
     gene            complement(4152..4880)
                     /locus_tag="PKK37_02615"
     CDS             complement(4152..4880)
                     /locus_tag="PKK37_02615"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNR81303.1"
                     /translation="MDKNTLVQFAKNIYEITLLFPKKEPLRHYLRQTADEAVAELVMK
                     KNDYAETLCRLFELADIQLEIAAGQNWVSPERIASLRDCLEQMSREFIAQKETIPASA
                     EEKYADKSCLGEAPAAPQNDAPIAGPNFVLVPSESPASLIPNLPRDLAPAGEPATAGE
                     KESENRESDQISLTAAQIARQNRIAEFLKEKGRAQVWEIQNIFPRVSKRTIRRDFRSM
                     LEQGLIERTGERNTTAYKLKINLS"
BASE COUNT         1135 a         1393 c         1175 g         1294 t
ORIGIN      
        1 ttggtcaagg tgaaaccttg ccatattcct tatgcggcgg tcataaatgc ggcgatccat
       61 tcttacaaag tttgcatcat tgcattcgta taggcaaaaa acaaaccgga agcggtccga
      121 ttcgtccata aatatctgac gcttttccac gccgcgattg aaaagatgat aaatatcgcc
      181 aatgataaac tctcgcgtga gatttgacat ataattttaa ttaaaattga agttgcaatc
      241 ttggcttcat tcttgattat agcacaaact tggtcaaggt ttcaccttga ccaattcaag
      301 cttggctgat tataaggcaa gagctctgcc ccgaagggag cagagctctt tgccgcgctt
      361 cgccacaaga gaagcgactt gactcaaact agcgcaagta aatcgccggg ctgatccacg
      421 ccgcttcgcc attcatataa cttgaagtta cgctggtcgg agtggtagac agatgccccc
      481 aattaatgga gttcacccga atatcatagg tgcccgcgac accaccatta ctggcagtgg
      541 ccttcaccgc gaacgtcgcc gagttccctt cgctaacctt ataggtgcca cccaccaaca
      601 ctgtgttgtc cggttggaca taagtcacat cattcagcgt tgcttgaaca ccaccaacac
      661 tggatttgat aacaaacgca tccgtgctgg tcgccgaaat gtagacatca ccgttcaccg
      721 ctttcacttg gatgttgaaa gtcgccgaca tcgtggaact cgcaccatca aacggagctt
      781 gcgtgttgtc cgtagtaata ctggccaaag tgaattccgg accctcggca tagatatacg
      841 ccgcgttgct ggtcgcagtt cccgtgctcg tgccagtggt gccattgctc ttggtgaaac
      901 caactttgcc cgtgccgatc gcaaccgtat aggtgtctcc atcatcggcc gacacacgag
      961 taccggtatt aggcgccgcc aaagtatcgt caaccttgat tgacagagtc ttggtcgtgt
     1021 ctttggagat ggtgtagtcc aattcggtga agtcattctt ttcggcagta gcgctggggc
     1081 taatcgtgcc gatcaccgtg ccgccatcgt caaccacata ggcagtcgcg ggaatctgtg
     1141 acgcaccaaa gtcaacatcc aaaatctcgt caatgatcaa atcgtccttg ctggccttca
     1201 aatcaaaggt catcaatgtc gcaccgctga tttcctgatt ggaatcggcg acaatattcc
     1261 gcttcttggg gctgttggca tttaagctca acgccaaagt cgcgttcgcg gcctgcgatt
     1321 cgttgacagt gaaactgcga tttacatcac catattccac gccgccgtat tggttcaaac
     1381 cggcctggtc aaccgcgcgc accgcagttg cggaaggcat gccgatgttg atcgtttggc
     1441 tggtattagt ggtttggaac aaaccgctgg aaataccggg caagacatcc aactttaccg
     1501 tcaacacctt gtcggtgttc accgggacaa tcactttgct ggtgaaaccc gccaaagtga
     1561 tttgatagtt ggtgctggta accttggaaa ccgtggttga attcaacgca ctggacgcaa
     1621 cctgcgtatc gccgtcatac aaatacacgt tggtaaagta tgaataaggc tgagcgccaa
     1681 attgcaaagt cacgcgctga acgtcaacat cggaaccggt ggctttcact ttcagaccca
     1741 tcaccgccac tttttcgtca ccttcgtagg tcttggtacc gttggccggg ctgggattaa
     1801 tggaaaccgt gatggaacct tcctgaccgg tcatggtcaa gccagtgccg gtggaagtgc
     1861 tcgtggacga gcaggactca cccgtaaccg ggctgtaacc cgcggtggaa gtgcaacctt
     1921 caggcaacgt ggtgccggtg gtaccggtgg tgccagtttc gcccgtggtg ccagtcgtgc
     1981 cgcccaccaa catcgcgttc aatttcgcgc gagtggaagc acccacaaaa ccggtgcccg
     2041 cggtcaaacc gaccggagcc aaaatttccg tggcgtattt gttttgaaac gccaccaccg
     2101 cggctttggt caaaccgcca aaatactgag attcattgcc cgccgaaccc acgccggaag
     2161 ccgccacttg cgtcgccgga tcctgattca acagagcttg caaacactgc acatcggttc
     2221 cctgcgaacc caatgtcaaa ttcctcgtga agctgatgcc cgcgcacgcc gccggagcgg
     2281 ttgaggaagt cgtgcccgtg gtagtcgtgg tgcctcccaa ttgtcccatc aaggacgcca
     2341 attgcgcctg aagctgagca atttgagccg ccagttcttc ggcagtcgcc gcttgtcccg
     2401 cggacggaac aatcgcggcc gccatcgcca acgcaaccgc gccggcgaat aatttcttgc
     2461 tattcattgt ttattttttt gttttacaca ggttatgagt caatgccata accttcttaa
     2521 ctttttgtcg cgccgacctt ttttccgcga aaccgcgaaa ttcctccccg ccaaagcttt
     2581 aaagcggtga agatcgcgac tgcttattct ccttttgccn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnt ttgaatagtc gcccgcggaa tataatttcc gcacttcgtt
     2761 gagatccact atctcctctt gggaaagcat ttcctcggca ttcaaataga gaatcagcga
     2821 ttcaataatc tgcttgtcca aaaccacctc ggcgcgcaaa tcttcttccc cggcgcccgg
     2881 ttccgcgccc agcacggtat aaacctgctt ttcccggtcg ttaatttctt tcatcgcccg
     2941 cgccgcctgc ttggcaattt cgccggcttt ttggggattc tttttcacca gcgcggccac
     3001 ttcttttttg gcttcggtct tggccgcctg atactcatcc atcgccgccg gcaagttctg
     3061 tgccagattc cggcgggaga taatgtcaat ctcttccaga cgcttggcgg ccaaccgcag
     3121 attcgccgcg gcagattcgc cgcgcctaac ctgtcccatc accatcgcgc ttcctttttc
     3181 ggtggctttt ttcaatccgt agagcggttc tcccggcaag gcattctgcg ccgaaaccaa
     3241 caatcccccg cccgcgcccg ccgccagaat caatccggcc aatgccgcgc ggtattgaaa
     3301 aacaaagcgg cgcaaaaaga tcatcggcga gctttttgcc gccatcgctt ccggacccat
     3361 aatgcgcgtt ttgcacgaaa ccacccaatc attgtcaggc gcaatttccc gtaaagtcct
     3421 tattcggttg atcaatgctt tttcggagtt catatttttt gggctcgcac tgctgatgac
     3481 gcaaaacccg cgaaaatgtt acacttcccc caaattctcc aaaagggtcg gaccctttta
     3541 acttctttgg ccggtatttt aggagagata ggtttttagt tctttcatcg cgcggtggag
     3601 caaaacccgc gtcgcctctt cggaacggtc caggagttgg gacacttctt tgattgatag
     3661 gtcatcaagg taacgccaga taatggcatt ctgataatcg tcgttaagat gggacatcgc
     3721 ctgccggaca gtgttcattt cagatgccaa catcgctttc ttttccaaat caatttttgg
     3781 atccggcaaa taattttcca ccgtttcggg cggcacagtc ctgatttttc ccgcggcacg
     3841 gtaatggtca accatcagat ttcgggcggt tttatacaaa aacgcgcgcg gattttggac
     3901 ggcgccggga tcctttttga atttttccca gcaccgcgca aacgcttccg cggtcaaatc
     3961 ttgcgccact tccttggagt tcacccgcaa aaaaacgaag cggtagactt ttttggcgca
     4021 atcatcatag agacggctga atttttcttg gtttttatcc atgccaaaca tcttgacatc
     4081 ttatcacatt tttgttctaa aatcaaccaa ttccacggaa aatttgcggg aaaattccgc
     4141 acgagcaacg cttaagacag attgattttc aatttgtagg cggtagtatt gcgctcgccg
     4201 gttctttcaa tcaatccctg ttccagcatt gaacgaaaat cccgccggat tgtgcgcttg
     4261 ctcacgcggg gaaaaatgtt ttgtatctcc caaacctgcg cccgcccttt ttcctttaaa
     4321 aattccgcga tgcggttttg ccgcgcgatc tgcgcggcgg tcaggctgat ttgatcggac
     4381 tcgcggtttt cgctctcttt ttctccggca gtggcaggtt cccccgccgg cgccagatcg
     4441 cgcggcaagt tcggaatcag agacgcggga gattcagacg gcaccaaaac aaagttcggg
     4501 ccggcaatgg gcgcgtcatt ttgcggcgcc gccggcgctt cacccaaaca ggacttatcg
     4561 gcatatttct cttccgcgga cgccggaatc gtttcttttt gcgcgataaa ttcccggctc
     4621 atttgctcaa ggcaatcccg caaagacgcg attctctccg gcgaaaccca attctggccg
     4681 gcggcaattt caagttgaat atccgccaat tcaaaaaggc ggcaaagagt ttcggcataa
     4741 tcgttttttt tcatcaccaa ctcggcaacc gcctcatcgg cggtttggcg caggtaatgg
     4801 cgcaatggct cttttttggg aaaaagaagc gtgatttcgt aaatattttt tgcaaattga
     4861 accagcgtgt ttttgtccat aatcgttttg tcctcccgac agcaaagcgg caccatcaat
     4921 tatctttata cgctttttta gccgaattgt ccaaaacttt accgacaacc actcaccagg
     4981 tctaaaatta aaagggtcta tccctcgtta ctgcgaggga tagtcccttt taattttgtg
     5041 ctcaaaattc ttttgccagg gtctaaccgc tcgcgattat ccctcggatt acctcgg
//
LOCUS       DAOQWE010000054         5046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_16870_length_5046_cov_5.128632, whole genome shotgun sequence.
ACCESSION   DAOQWE010000054 DAOQWE010000000
VERSION     DAOQWE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16870_length_5046_cov_5.128632"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2065
                     /locus_tag="PKK37_02620"
     CDS             <1..2065
                     /locus_tag="PKK37_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNR81304.1"
                     /translation="DNLTLTTRANEQAGDFGTAATTTATTLTDSGKAWTADEWIGGTI
                     TIVAGAGKGQTQTITDNDATVITVADWDATLGDPSANSVYRLSYAPAGDVLANIANAN
                     AIFGGFKVLGNDNGGYAFRVAPDGSVHIGGEGAGEASLFVKQNITAYGGILSLRQLDI
                     SSAGTPVATTATSGGSCADSTAYYYKVTAINGNGETTGTAQFTVTTGASGTNINLNTI
                     AWSAVTGATGYKIYRSTENNDWDGDANDQWVDGKVVAAPAITYDDDCAGDTASLVPPT
                     ANTTGGKLAINTAGVSPTRRFEVLENTGAAPQMKIISDSSNYSEFYVDATGDLSLKLT
                     GSGGDDLILLDENLKICAGGDFGAVSCPTAGFSLSGTGNLIVENKVIADKFEQICPTG
                     YVWVPGSAKHGTLPGFCVMKYEAKDAGGGVAESKAADAPWTSVTQENARAYCQALGEG
                     YHLVSEAEWMTIAENIAATPINDIDAVAGLQLATGHTDNDPANSLAAGTDPVVSGCNL
                     RLPLSDAANAFDADCQLRDNGGEVYGYSGTGGNFWNDIGYLAGGNNKSQLRVHALSNG
                     NTVWDIAGNVYEWTDAIISQVDQPGISNDGGAGVANWGEWNVSYYLVGAAANSRPPDD
                     GWTGANGIGRLYTYGRDETATTTYRAFQRGGNWAGTSYAGVFALALNDSPAYSNGNIG
                     FRCAR"
     gene            2338..2553
                     /locus_tag="PKK37_02625"
     CDS             2338..2553
                     /locus_tag="PKK37_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNR81305.1"
                     /translation="MRNVVSISLPKQLDEQIEKEVKTGRFASKSEFIRSLIRLWQEEK
                     LFRELEQGRKEIEAGLGKELKSLKDLR"
     gene            2580..2840
                     /locus_tag="PKK37_02630"
     CDS             2580..2840
                     /locus_tag="PKK37_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HNR81306.1"
                     /translation="MIIIYSPHFERLYRRLPGDVKDNAELAEEIFRNNPQDARLRVHK
                     LSGRLSGYWAFSVNFEYRIIFYYGNSKEEIFFDSIGNHDIYR"
     gene            3125..3433
                     /locus_tag="PKK37_02635"
     CDS             3125..3433
                     /locus_tag="PKK37_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR81307.1"
                     /translation="MSTIEAKKIVKKYAQRLRKNQIPFSAIYLFGSFAKGQERETSDI
                     DVAIVSKRKETDRFIVEVGRPIWDVDTRIEPHIISEEDFKEINTSIVEEIIKTGIKVE
                     "
     gene            3639..4877
                     /locus_tag="PKK37_02640"
     CDS             3639..4877
                     /locus_tag="PKK37_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81308.1"
                     /translation="MISINNLEEIARDQEEKFRQRDRGIARDIDFEKYLKTAQIVVIS
                     GARRCGKSTLLRQFYDNFGSCHYLNFDDERLLDFEVADFQNLMLAFGKINDSKTILLD
                     EIQNIGSWERFARRIHDEGYKIFITGSNSKLLSSELATHLTGRYFGLELFPFSFKEFL
                     NFKKIDWQVLNSRKRIEILSALDDYLQNGGFPEFLKGQDVEYLKKIYEDILYKDIIAR
                     FKIREVKSFRELANYVFSNFAQETSYNGLKNVLGFKSLTTVKNYIHYLQESYLAFEVF
                     KYDFSLKKQYVSNKKIFVVDNGLRNSVAFGFSEDRGRLLENLIFLELKRRGKEIYYYK
                     DKKECDFLIKEKNKITGACQATKELSPANEKRELGGLREAMDNFGLDEGLIITEGHEE
                     ERKIDGKKIRVVSLCKWLLE"
BASE COUNT         1390 a         1165 c         1267 g         1224 t
ORIGIN      
        1 cgacaatttg acgctcacca cccgcgccaa cgagcaggcc ggcgatttcg gcaccgccgc
       61 gaccaccacg gcgacaacct tgaccgattc gggcaaggcg tggacggccg acgagtggat
      121 cggcggcacg atcacgatcg tggccggcgc gggcaagggg caaacgcaaa ctatcaccga
      181 caacgacgcg actgtcatca cggtggcgga ctgggatgcc acgctgggcg atccatccgc
      241 caactcggtc taccgcttga gctacgcgcc ggcgggcgat gtgctggcaa acattgccaa
      301 cgccaacgcg attttcggcg gcttcaaggt gctgggcaat gacaacggcg gctacgcgtt
      361 ccgcgtggcg cccgatggca gtgtgcacat cggcggcgag ggcgcggggg aagccagtct
      421 gtttgtcaag caaaacatca ccgcctacgg cggcatcttg tctttgcgcc agctggatat
      481 ttcttcggcc ggcacgccgg tcgcaaccac tgccaccagc ggcggttcgt gcgcggattc
      541 cacggcttat tactacaagg tcaccgcgat caacggcaat ggtgaaacca ccggcacggc
      601 gcaatttacc gtgaccaccg gcgcgtcggg caccaacatc aatctcaaca ccatcgcctg
      661 gtcggcggtc accggcgcca ccggttacaa gatttaccgc tccaccgaaa acaacgactg
      721 ggacggcgac gccaacgatc agtgggtgga cggcaaagtc gtggccgcgc ccgcgatcac
      781 ctatgacgac gattgcgccg gcgacaccgc ctcgcttgtc ccgcccactg ccaacaccac
      841 cggcggcaag ctggcgatca acaccgccgg agtttctccc acccgaagat ttgaagtttt
      901 ggaaaacacc ggcgccgcgc cgcaaatgaa gattatttcc gattcttcca attattccga
      961 gttctatgtt gacgccaccg gcgatttgag cttgaagctc accggttccg gcggcgacga
     1021 tttgattctg cttgacgaaa acctgaaaat ttgcgccggc ggcgactttg gcgcggtttc
     1081 ctgtcccacg gccggattct cgctttccgg cacgggcaat ctgatcgtgg aaaacaaagt
     1141 catcgccgac aagtttgaac aaatttgccc caccggctat gtctgggtgc cgggttcggc
     1201 caaacacggc accctgcccg gcttttgcgt gatgaaatac gaagccaaag acgcgggcgg
     1261 cggcgtggcc gaaagcaaag ccgccgacgc gccgtggaca agcgtgacgc aggaaaacgc
     1321 ccgcgcctat tgccaggcgc tgggcgaagg ctatcatctg gtttccgaag ccgaatggat
     1381 gaccattgcc gaaaacatcg ccgccacgcc catcaacgac attgacgccg tcgccggcct
     1441 gcagttggcc accggccaca ccgacaacga tccggccaat tcgctggccg ccggcaccga
     1501 tccggtggtt tcggggtgca acctgcgcct gccgctttcg gacgccgcca acgcatttga
     1561 tgccgattgc caattgcgcg ataatggcgg ggaagtctat ggctattcgg gcaccggtgg
     1621 taatttttgg aacgatatcg gctatcttgc cgggggcaac aacaaatccc aattgcgtgt
     1681 ccacgccctg tccaacggca acactgtctg ggacatcgcc ggcaatgttt atgaatggac
     1741 cgacgccatc atttcgcaag tggatcagcc cggcatcagc aacgacggcg gcgcgggggt
     1801 cgccaactgg ggcgagtgga atgtctccta ttatcttgtc ggcgcggcgg caaactcaag
     1861 accgcccgac gacggctgga ccggtgccaa cggcatcggc cggctataca cctatggccg
     1921 cgacgaaacc gccaccacga cttaccgggc ctttcaacgc ggcggtaatt gggccggcac
     1981 ttcctacgcc ggcgtcttcg ctctggccct gaacgattcg cccgcgtatt ccaacggcaa
     2041 cattggtttc cgctgtgccc gctgacgggg tttatgtatt tatctaaaat ctaatgtctg
     2101 aaaatctaaa atcttgaatt ggcatgttaa aataagttca aatgacaaaa accaaatgtc
     2161 aaatcaatgt caaaaatcaa atgataaagt aaatgcggta tttgttttga gtttttagtt
     2221 ttggatttga ttggacattt ggattttgag ctttggcatt atgcgtatcg gaggagcggt
     2281 ttgacaagcg atttgggata tgtaatactt tgtattacga ttaacaaatt taaatctatg
     2341 cgcaatgttg taagcatctc gcttcccaaa caattggacg agcaaataga aaaagaagta
     2401 aaaaccggca ggtttgcctc caagagcgaa ttcatccgca gcctgattag gctatggcag
     2461 gaagaaaaat tatttcgcga actggaacag ggaagaaaag aaatagaggc aggcttggga
     2521 aaagaattaa aatcccttaa agatttgcgg taagcaataa ttaatttcat tcaattgaaa
     2581 tgataattat ttactcgccg cattttgaac ggctttacag aagacttccc ggcgatgtaa
     2641 aagataatgc cgaacttgcc gaagaaatat tcaggaacaa tcctcaagat gcccgtttgc
     2701 gcgtccataa actcagcggc cgattgtccg gctattgggc gttttcggtt aactttgaat
     2761 atcgcattat tttttattac ggcaacagca aggaagaaat attttttgat tctatcggca
     2821 atcacgatat ctacagataa agggcaattt ataacagcca tttaaaagat tttatcaatt
     2881 ttttcagagg gtctaaccct ctgaagatcc tttaaaaaat gggaaaaatc aatgatcaat
     2941 aatccggcaa tcttttttta aaagggtcgg acccttttaa agccgcgccg gcggaaaaga
     3001 ttccggcaag aatgattttt tgctatcatc aagacagaag cttgtcgcgt tagttggcgc
     3061 gaaggttaga ccttcgcatt gatcaaaaaa atttagataa aattgaaact atttataaaa
     3121 atttatgtca acaattgaag caaaaaaaat cgtaaaaaaa tacgcgcaaa gattgcggaa
     3181 aaaccaaata cctttctcgg ccatctattt gttcggttct ttcgcgaaag gccaggaacg
     3241 ggaaaccagc gatattgatg tggcgattgt atcaaaacgc aaagaaactg atagatttat
     3301 agttgaagtt ggacggccga tttgggatgt tgatacaagg attgagccgc atataatctc
     3361 ggaggaagat tttaaggaaa taaacacttc tatcgttgag gaaatcatta aaaccggcat
     3421 taaggtagaa taatagcgta ttcgctctta gtcatctaat ttgtgagatc agcaggttgg
     3481 ataaaaaaca aagaatccgc gttttcgcgc ggattcttac ggagtggtaa cactccggtg
     3541 agcggttact ctttgaaaac ttgaccggcg attctaatta tatatactac taatatagat
     3601 aatatcaatt tatctatatt ggtaatgtag ataatctaat gatttcaata aacaatttgg
     3661 aagaaatcgc cagagatcaa gaggaaaaat tccgtcaacg agaccggggg atcgcgcggg
     3721 atattgattt tgaaaaatat ttaaagaccg cgcaaatagt tgttatttcg ggcgcgcggc
     3781 gctgcggaaa gtcaacgctc ttgcggcaat tttatgataa tttcggcagt tgccattacc
     3841 ttaattttga cgatgagcgg cttttggatt ttgaagtcgc cgatttccaa aatttaatgc
     3901 tggcgttcgg caaaattaac gattcaaaaa cgattctgct ggatgaaatt cagaacatcg
     3961 gcagttggga gaggttcgcg cggcggattc atgatgaagg atataaaata tttataaccg
     4021 gatcaaattc caagctgttg agttcggaat tggcaaccca tttgaccggg cgctatttcg
     4081 gacttgagct ttttccgttt tctttcaaag aatttttgaa tttcaaaaaa attgattggc
     4141 aagttttgaa ttcgcggaaa agaattgaaa ttctgtcggc gctggacgat tatttgcaaa
     4201 acggcgggtt tcccgaattt ttgaaaggcc aagacgtgga atatctcaaa aagatatatg
     4261 aagatatttt atataaagat attatcgcgc ggtttaaaat tcgcgaggtg aaatcatttc
     4321 gcgagcttgc caattatgtg ttttcaaact ttgcccaaga aacgagctat aacggattaa
     4381 aaaatgtttt ggggttcaaa agcttgacaa cggttaaaaa ttatattcat tatttgcagg
     4441 aaagctatct ggcgtttgag gtttttaaat atgatttttc tttgaaaaag caatatgttt
     4501 ccaataaaaa aatttttgtc gttgataacg gcttgcgcaa ttccgtggcg tttggatttt
     4561 ccgaagaccg gggccgcctg ctggagaatt tgatattttt ggaactaaaa aggcgcggca
     4621 aggaaattta ttattacaag gataaaaaag aatgcgattt tttgatcaag gaaaaaaaca
     4681 aaatcaccgg cgcgtgccaa gcgaccaaag aattgtcgcc ggccaacgaa aaacgggaac
     4741 tgggcggcct gcgggaggcg atggacaatt tcgggcttga cgaaggcctc attatcaccg
     4801 aggggcacga ggaagaaagg aagattgacg gcaagaaaat ccgcgtggtt tcgctgtgta
     4861 aatggcttct ggaatgattc gccgagattt tcaaacccat tttccaaaag ggtcggaccc
     4921 ttttggaaag ggtccgaccc tttcaaacct tggcgcgctt ccggcaagaa tgattttttg
     4981 ctatcattaa gatatgaaaa cggcgcaaaa caattatgcg tttattgacg cggcaaatct
     5041 ttttta
//
LOCUS       DAOQWE010000055         4978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_17174_length_4978_cov_6.616291, whole genome shotgun sequence.
ACCESSION   DAOQWE010000055 DAOQWE010000000
VERSION     DAOQWE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17174_length_4978_cov_6.616291"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PKK37_02645"
     CDS             <1..1372
                     /locus_tag="PKK37_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNR81309.1"
                     /translation="NIKTINAKTAPPPANLLREGLLLGFNQYRGTKTDVYLGPDDRRR
                     HLYVIGQTGTGKSAFLSNLIEQDIIAGRGVGILDPHGDLIDDIMGKIPNDRVDDVVVF
                     DPSSREYSIGLNMLEYDPEFPEQKTFIVNEFMKIFDKLYDLKATGGPMFEQYARNALQ
                     LLMDDPKETYTLMEVPKVLADKEFRHYLLSKCRNIMVREFWEKQAEAAGGEASLKNMV
                     PYITSKFDTFISNDYMRPIIGQVKSTFKFREILDQKKIFLVNLSKGRLGEINSSLLGL
                     IITGKITIAAFSRVDVPESERRDFHLYIDEFQNFATDSISTILSEARKYKLCLTVSHQ
                     FIGQLEEKIRDSVFGNVGTISSFRVGAEDSEFLAKQFAPVFNASDLSRLENYNAYVRI
                     LINGQVSLPFSIKTYPPSCANRSRSADIREYYLVKEGRPREMVEREIEARRRQMGAPA
                     GKNANN"
     gene            1381..1776
                     /locus_tag="PKK37_02650"
     CDS             1381..1776
                     /locus_tag="PKK37_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81310.1"
                     /translation="MPDQKMPSPETPAIKKIEQLEKEAEFERENEGLEVIEPGAKTAE
                     ALRARVEKSFPEASEKEPDKKIIGKDIEKKIETIVNNFVKSAAAAEFDEGKVDQIIGK
                     ARMYIAKGYPQIADEVHDRILEAKNKGMH"
     gene            complement(1883..2341)
                     /locus_tag="PKK37_02655"
     CDS             complement(1883..2341)
                     /locus_tag="PKK37_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81311.1"
                     /translation="MKTFSSFLNNMNVCAVCFDGENCQSLKLAAIFCRLRRNNEPKSA
                     IFCRDPRRIQPATDLHSSKTGRNFYSSFPLVSIMAANSVHVKIIKLLTGGVAVFDTAT
                     IPIFNISEFREIMYFWHNRNRPLLIFFAIAKNIKFKKIIGYIKKEKVINL"
     gene            2444..2689
                     /locus_tag="PKK37_02660"
     CDS             2444..2689
                     /locus_tag="PKK37_02660"
                     /inference="COORDINATES: protein motif:HMM:NF039814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81312.1"
                     /translation="MGKNFIKKNIKLSLEFDRYLAKNPATFEKIPNNACLVLTVEGDD
                     AFNQESKAMVRIAKSQRQKCVEARKEGERWVLGPLAV"
     gene            2860..4467
                     /locus_tag="PKK37_02665"
     CDS             2860..4467
                     /locus_tag="PKK37_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNR81313.1"
                     /translation="MKLNKRFWIIAIAAAVAGLLLWNIFKPKEADYKTEAVVIGSITQ
                     EVAETGSVRKGEAINLNFKNSGIIEKISVSQGEEVSAGQVLAELDNRQAQIQMTQAKA
                     NYEATLFQLEKLKRGAGGEDVAIVESQVLAARAALDAARRAQEDARAVADQGLSAVYK
                     TAADSLASAYIRAYNAYNFSDLLQREYFSPRDEDSIAVWEMLQKMKLAVDKIKIYSQT
                     AQADGKDAGLDADFAAAKTELAAVEARLREIRVMCEKIPWRDAVSLTHKENLDLHIGY
                     VSAARAAFHSAMEGVASQKASGDLAVNSAAAALEGAQAAEKTASEQYAKVLAPPRAED
                     VGMLEAQTAQAQAQIALLQLQIDDGKLTAPADGRIAEINGRAGETVSPLAGEPLMVLF
                     PADPYQIVIDIYEEDAVKVSIGDLCKISVAALGDQTFSGRVAFISPAAKIVNGVVYYE
                     TKIAFDQSPEGLMPEMTADVEIITAQKENVLLVSEAALRKREDGWKVEVVGDDATREV
                     GVQIGIRAKGIAEIISGIAEGDLVVIP"
BASE COUNT         1382 a         1055 c         1320 g         1221 t
ORIGIN      
        1 caatatcaaa acgatcaacg ccaaaaccgc gccgccgccg gcaaacttgt tgcgcgaggg
       61 cctgcttttg ggatttaacc aatatcgcgg aacaaaaacg gatgtttatc tgggccctga
      121 cgatcgccgc cgccatttgt atgttatcgg acaaaccggc accggcaaat ccgcgttttt
      181 gtccaacctg attgagcagg atataatcgc cggacgcggc gtggggattt tggatccgca
      241 cggcgatctg attgacgaca ttatgggtaa aattcccaac gaccgcgtgg atgacgtggt
      301 ggtctttgat ccttcaagcc gcgaatattc catcggcctc aatatgttgg aatatgatcc
      361 cgaatttccc gaacagaaaa ctttcatcgt taatgagttt atgaagatct ttgacaagct
      421 ctatgatctg aaagccaccg gcgggccgat gtttgagcaa tacgcgcgca acgccctgca
      481 actgctgatg gacgatccca aagaaaccta cacgctgatg gaagtgccca aggttttggc
      541 cgataaggaa ttccgccatt atctgttgtc caaatgccgc aatattatgg tgagggaatt
      601 ttgggaaaag caggctgaag ccgcgggcgg agaggcgtcg ctgaaaaata tggtgcccta
      661 tatcacttcc aaatttgaca ctttcatttc caatgattat atgcgtccca tcatcggaca
      721 ggtgaagagc actttcaaat tccgcgaaat tttggaccaa aaaaagattt ttctggtcaa
      781 tctgagcaag gggcgtttgg gcgagatcaa cagttcgctt ttggggttga ttatcaccgg
      841 caagatcacc attgccgcgt tttccagagt ggatgttccc gaaagcgagc gccgtgattt
      901 ccatctctat attgacgagt ttcaaaattt tgccaccgac agcatttcca ccattttgtc
      961 cgaagcgcga aaatacaagc tgtgcctcac cgtttcccat cagttcatcg gccaactgga
     1021 agagaagatt cgcgacagcg ttttcggcaa tgtgggaacg atttcgtctt tccgcgtcgg
     1081 cgcggaagac tcggagtttt tggccaaaca attcgcgccg gttttcaacg cgtcggattt
     1141 gagccgcctt gaaaattata acgcttatgt aagaatattg attaacggtc aggtatcgtt
     1201 gccgtttagc attaagacct atccgccgtc ttgcgccaac cggtcccggt cggcggatat
     1261 ccgcgaatat tatctggtca aggaaggccg tccgcgcgag atggtggagc gcgagattga
     1321 agcgcgccgc cgccaaatgg gcgcgcccgc cgggaaaaat gccaataatt gataaaaatt
     1381 atgccggatc aaaaaatgcc gtcgccggaa acgccggcaa tcaaaaaaat tgaacaattg
     1441 gaaaaggaag cggaatttga gagggaaaac gaaggattgg aagtgattga gccgggggcc
     1501 aaaaccgccg aggctttgcg tgcgcgggtg gaaaaatctt tccccgaagc ttcggagaaa
     1561 gagccggata aaaaaatcat cgggaaggac attgagaaaa agatagaaac cattgtcaat
     1621 aattttgtga aatcggcggc ggcggctgaa tttgatgagg gaaaagttga tcagattata
     1681 ggtaaggcaa gaatgtatat cgccaaaggc tacccccaaa tcgccgacga agtccacgac
     1741 cgcatcttgg aggcgaaaaa caaaggaatg cattgatttt taacttttgg cataaatgtc
     1801 gcaaggttga accttgctgt gtttgggttc agcgtccttg atttttggcg ccgattcgct
     1861 gaatctctaa atgtccagaa tattaaaggt taatcacctt ttcttttttt atatacccga
     1921 tgattttttt aaatttgata ttttttgcta tggcaaaaaa tatcaaaagt gggcgattcc
     1981 ggttgtgcca aaaatacata atttctctga attccgaaat attaaatatt ggtatcgtag
     2041 cggtatcaaa taccgctact cccccggtaa gtaatttgat tatcttgaca tggacggagt
     2101 ttgcggccat tatggaaaca aggggaaatg aagaataaaa attacgtcca gtttttgacg
     2161 aatgtagatc ggttgcaggt tgaattcgtc gaggatcgcg gcaaaatatt gcgcttttcg
     2221 gttcattatt gcgccttaat cggcaaaaaa tggcggccaa ttttaaggat tgacaatttt
     2281 caccatcaaa acaaaccgca cagacattca tattatttaa aaaagaagag aatgttttca
     2341 tattggggcg gcgatgccaa tgaggcgttt accgaagcca aaaaatatat cgttaaaaat
     2401 tacgccaaaa tcaaagaaaa ttatttttta actgcttgaa tttatgggta aaaattttat
     2461 caaaaaaaat atcaaacttt cattagagtt tgaccgttat ttggccaaaa atccggcaac
     2521 gtttgaaaaa ataccgaata atgcgtgctt ggtgctgacg gtggagggcg atgatgcttt
     2581 taaccaagaa agcaaggcaa tggttaggat tgccaaaagc caaaggcaga aatgcgtgga
     2641 ggcgcgcaaa gaaggtgagc ggtgggtttt ggggccgctg gcggtttgat ttttcgccaa
     2701 gcgcgccggt ttgaaaattt taacggaaag ccgcggcata ggccgcggct tttttgttgt
     2761 gtcatttttt gttgtgttat tttgttgtgt tcaaaacgga aaaaatgagg tagaatggaa
     2821 caatacattt aagtgtgtgc gcgataaaca tagaatcata tgaaattaaa taaaaggttt
     2881 tggataattg cgattgcggc cgcggtcgcc ggacttttgc tgtggaacat attcaagccc
     2941 aaggaggcag actacaagac cgaggcggtt gtcattggat caatcacgca ggaggtggcc
     3001 gaaacgggga gcgtgagaaa gggagaggcg atcaatctga atttcaagaa cagcggcatc
     3061 attgagaaaa tcagcgtgtc ccagggggaa gaagtgtccg cgggacaagt gctggcggag
     3121 ttggacaatc gtcaggcgca aattcaaatg acgcaggcaa aagcgaatta cgaagcgacg
     3181 cttttccagt tggaaaaatt aaaacggggc gccggcggtg aagatgttgc tatcgttgaa
     3241 agccaggttc ttgccgcccg cgccgcgctg gacgccgccc gccgggcgca agaagatgcg
     3301 cgcgcggtgg ccgatcaggg gttgagcgct gtttataaaa cggcggccga ttcgctggca
     3361 tcggcttata tccgggcata taacgcctat aatttttccg atcttctcca gcgcgaatat
     3421 ttttcgccgc gcgatgaaga ttccatcgcg gtttgggaaa tgttgcaaaa aatgaaattg
     3481 gcggttgaca agattaaaat ttattcgcaa accgcgcaag cggacggcaa agacgccggt
     3541 ctggacgcgg attttgccgc ggccaagacc gaattggccg cggttgaagc gcgtttgcgc
     3601 gaaattcgcg tgatgtgcga aaagattccc tggcgcgatg ccgtaagttt gacccataag
     3661 gagaatttgg atttgcatat cggctatgtt tcagccgcgc gcgcggcatt ccattccgcg
     3721 atggagggcg tggcgtcgca aaaggcgtcc ggcgatctgg cggtgaattc cgccgccgcc
     3781 gcgctggaag gcgcgcaggc ggcggaaaag accgccagcg agcaatacgc gaaagttttg
     3841 gcgccgccgc gcgcggaaga tgtgggaatg ctggaagccc agaccgcgca ggcgcaggcg
     3901 cagatcgctt tactgcaact gcaaattgac gacggcaaac tgaccgcgcc ggcggacggt
     3961 cggatcgcgg agattaacgg gcgcgccgga gaaacggtat cgccgctggc cggcgagccg
     4021 ctaatggtgc ttttccccgc cgatccttat cagatcgtca ttgacatata cgaagaggat
     4081 gcggtcaaag tcagtatcgg tgatttgtgc aaaatttccg tggccgcgct gggtgatcaa
     4141 actttttccg ggcgcgtggc gttcatttct ccggcggcca aaatcgtgaa tggcgtggtc
     4201 tattatgaaa ccaagattgc ttttgaccag tctcctgaag gattgatgcc ggaaatgacc
     4261 gcggacgtgg aaatcatcac cgcccaaaag gaaaacgttt tgctggtttc cgaggccgct
     4321 ttgcgcaagc gggaggatgg ctggaaggtg gaagttgtcg gcgacgacgc gacgcgggaa
     4381 gttggcgttc agattggcat ccgcgccaag ggaattgccg aaatcatctc gggcatcgcc
     4441 gaaggcgatt tggtggtcat tccgtaattt tttaataaaa aaagatttat tactccgttc
     4501 gcaaattttt gcgcaaatta cgggggaaaa taaatcttac tacggtcaat gattcgccac
     4561 ggaagatata aataaagctg agaaatataa aacttccgta ggcggagatt aaagccacga
     4621 ccgcccacac cccaattaaa taaaaagcta gatccgttgc gggggtgcgg agggcatgga
     4681 taaatattgc cacagtgcta gaaatgaata ggaaggggct aaccaacaac caaacaaata
     4741 ttatgcgtct gtttatcatt caatcacctt aagccagagt atagtaattg attttgtttg
     4801 tcaatagtaa ttatttttta ttgggattat ttgtttgtgc gtatcggtat tttaaaacga
     4861 tagaaataac cctgctaaaa tcaataaatt tttcaaaata accctgctgt tttttcatta
     4921 tgtcaacact gatcaaattg gaagaagttt ggaaaattta tcatctgggg aagacgga
//
LOCUS       DAOQWE010000056         4930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_17396_length_4930_cov_4.817641, whole genome shotgun sequence.
ACCESSION   DAOQWE010000056 DAOQWE010000000
VERSION     DAOQWE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17396_length_4930_cov_4.817641"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PKK37_02670"
     CDS             <1..300
                     /locus_tag="PKK37_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81314.1"
                     /translation="KEVLTKKWTDWIDYWSVDFEFESKKEIVRVVDEKTGEEKEEWTG
                     SYIFENEWQSFRTKKDRSLELTSAFKDAQKGKMKIAIKVIDIFGNDTTKVIEVNI"
     gene            305..>1609
                     /locus_tag="PKK37_02675"
     CDS             305..>1609
                     /locus_tag="PKK37_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81315.1"
                     /translation="MALHKNFPKDKFQILDPSIRWFPADEDLRKEGYEKLLPPFVPEL
                     RERVAEWRTNNYEGASETSKSLLNWWFKEPHTIFAKDGTAIPFQYYYAQREAVETIIW
                     LYDVEKVVGKYDLIKFDKLGRVSPNMFTEDWLRFVIKMATGSGKTKVMSLLLAWSYFH
                     KLYEENSELAKNFLLITPNIIVLDRIKTDFEGLKIFYEDPILPDNGYGGQNWQDDFQI
                     KLHLQDEVGTISKSGNIFLTNIHRVYEGSIDEASFEDEDTSDYFLGSKVVGKTNDSKI
                     DLGEIVRDVDELMVINDEAHHIHDPKMAWFKSIEDIHNQLLQKGKKLALQIDVTATPK
                     NNRGEIFVQTVSDYPLVEAIHQGVVKNPVLPDSASRAKLQEKQSSIFSEKYEDYIRLG
                     VEEWEKTYKELEPTGKKSILFIMTDDTKNCDDVAEFLEKSYPQ"
     assembly_gap    1610..1709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1710..2834
                     /locus_tag="PKK37_02680"
     CDS             <1710..2834
                     /locus_tag="PKK37_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HNR81316.1"
                     /translation="GRGLRRMFFGQDVEEYVSVIGTPAFMEFVESIKAEGVELEKRKM
                     DRSGTPITPTVIEVDTQNVKKDIENLDIELPVLTPRIQREYKNLGLLNVAEFKHERIS
                     IKEFSEEEKKEIVFKEVIDDKLHHTTILNSTVEPNYQSVVGFFVQRIMKELRLFGCYD
                     ILFGKVKEFIGDELFSRQVDLNDANILRNLSELEAVKTIVETFKKEINNLTVQDVGDT
                     EIKNYLKISSARPFVVTDKKYLLPKKSVFNKIVGDSDFELEFADFLEGADDVLSFAKN
                     YYEIHFKIDYKNASGAISSYYPDFFVKTDNKTIYIVETKGREDLDDVEKIKRLEQWCE
                     DASERQKKIAYKMLYVKQEEWDKYKPTSFRQLAEVFGNKA"
     gene            2837..4741
                     /locus_tag="PKK37_02685"
     CDS             2837..4741
                     /locus_tag="PKK37_02685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNR81317.1"
                     /translation="MDDIKTTIHGDATSDQIKAIDHVGSHARLLAGPGTGKTKTLTRR
                     VLSLILQHNADPETILLLTFTRLAAAQLKDEIKKVLEPLGKATPQVSTLHSFALRQIL
                     FNSSRVDALPRPIRIADDWEERHIIQEDLKRILNLKEIADVQKLINQLSTDWETLRVD
                     EIGWQQQFPNPAFLGAWQGHKNQYGETLRAELVYQLKRQLNQNRDFQLDKEYKHILID
                     EYQDLNACDLAVVNELAKRGAELFVAGDDDQSIYGFRFATPDGIRNFDQFYQNAQKLA
                     LEICFRCDKNILHSAEFVASLDPRRLPKPTRTKDEADDGEVLIIGFRDQVHEAESIAK
                     KIKSLIDAGTNPDQITILLRSDRNGALSKPIIEMLKKQNVDVSLSTDSELDNNNEYRA
                     VLSIIRLLVDQNDCLAWRTLLQIGKNGLGDECIKNIENYAQSKDIRFSAAIQKIAENQ
                     NELRKFGQKISAFVSGTKTIIEELKAVENPIEVINSLVDKFMTASEIKEQVKKYFIGI
                     LENQEKTDLENLIKAVSISSEFIEQETKENAVNILTMHQAKGLTFDVCFIVGAEDEFI
                     PGRNTGDKEGDERRLLYVSMTRARHKLFISYCNKRTGQQRHSGSNSGTEKRTLTRFLR
                     DSKIPIKQVK"
BASE COUNT         1679 a          837 c         1072 g         1242 t
ORIGIN      
        1 aaagaagttt tgacgaaaaa atggacggat tggattgatt attggtcggt tgattttgaa
       61 tttgaaagca agaaagaaat cgtccgcgtg gttgatgaaa aaaccggcga agaaaaagaa
      121 gaatggacgg gaagctatat ttttgaaaac gaatggcaat ctttcagaac caaaaaagac
      181 cggtcgcttg agttgacttc ggctttcaaa gacgcgcaaa aaggcaaaat gaaaatcgcg
      241 attaaagtga ttgatatttt cggcaacgac acgaccaaag ttattgaggt taatatttaa
      301 tactatggct ttacacaaaa attttcccaa agataaattc caaatacttg atccgtcaat
      361 caggtggttt ccggctgacg aagatttgcg caaagagggc tacgaaaaac ttttgccgcc
      421 ttttgtgccg gaactgcggg agcgtgttgc cgaatggcga acaaataatt atgaaggggc
      481 gagcgaaact tcaaaatccc ttttgaattg gtggtttaaa gaaccgcaca cgatttttgc
      541 caaagacggc acggcaattc cgtttcagta ttactacgct caaagagaag cggtagaaac
      601 gattatttgg ctttacgatg ttgaaaaagt cgttggtaaa tacgatttga tcaaatttga
      661 taaattgggt cgggtaagcc cgaatatgtt taccgaggat tggctccgct ttgtgattaa
      721 aatggcaacc ggtagcggaa aaacaaaagt gatgagtttg cttttggctt ggtcgtattt
      781 tcacaagctc tacgaggaaa attcggaact ggccaaaaac tttttactta ttactccaaa
      841 cattattgtt ttggatagaa ttaaaaccga ttttgaagga ttgaaaatat tttacgaaga
      901 cccgattttg ccggacaacg gctatggcgg tcaaaattgg caagacgatt ttcaaatcaa
      961 actacatttg caggacgaag tcggcacaat ttcaaaatcg ggaaatattt ttcttaccaa
     1021 catccacaga gtttatgaag gcagtatcga cgaagcgagt tttgaagatg aagacacatc
     1081 ggattatttt ttgggaagta aagttgtcgg caaaaccaac gattcaaaaa ttgatttagg
     1141 cgaaatagtc cgcgatgttg acgaactaat ggttatcaac gacgaagctc accatatcca
     1201 cgatccgaaa atggcgtggt ttaaatctat tgaagatatt cataatcaat tactgcaaaa
     1261 aggcaaaaaa ttggctctgc aaattgatgt tacggcaacg ccaaaaaata atcgcgggga
     1321 aatttttgtg caaaccgttt ctgattatcc tttggttgaa gcgatacatc aaggcgtggt
     1381 aaaaaatccg gttttacccg attccgccag ccgagcgaaa ctacaagaaa aacaaagctc
     1441 catattttcc gaaaaatacg aagattatat ccgattagga gttgaagaat gggaaaagac
     1501 atacaaggaa cttgaaccga ccggcaaaaa atcgattctt ttcataatga cggacgacac
     1561 gaaaaattgc gacgatgttg cggaattttt ggaaaaaagc tatccgcaan nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtcgcggtct tcgccgaatg ttttttggtc
     1741 aagatgttga ggaatatgta agcgttatcg gcacgcccgc ttttatggaa tttgttgaat
     1801 ctatcaaagc cgaaggcgtt gagttggaaa aacgaaaaat ggatcgttcc ggcacgccaa
     1861 ttacgccgac agttattgag gttgacaccc aaaatgtaaa aaaagatatt gagaatttgg
     1921 atattgaatt gccagtttta acaccgagaa tacagagaga atataaaaat cttggtttgc
     1981 tgaatgttgc ggaatttaag cacgaaagaa tatcaattaa agaatttagc gaggaagaaa
     2041 agaaagaaat tgttttcaag gaagtgattg acgataaact tcatcacaca acgattttga
     2101 atagcactgt tgagccaaac taccaaagcg tcgtcggatt tttcgtgcaa agaataatga
     2161 aagaactgcg cttgttcggt tgctacgaca tactttttgg caaggttaag gaatttatcg
     2221 gcgatgaatt atttagccgc caagttgacc tgaacgacgc aaatattttg agaaatttat
     2281 ccgagttgga agcggtgaaa actatcgtgg aaacattcaa aaaggaaata aacaatttga
     2341 ccgtgcagga tgttggcgat acggaaataa aaaattatct caaaataagc agtgcaaggc
     2401 cgtttgttgt taccgataaa aaatatttgc tacccaaaaa gagcgtattc aataaaattg
     2461 tcggcgatag cgatttcgaa cttgaatttg ccgacttttt agaaggtgcg gacgatgtgc
     2521 tttcctttgc caaaaactat tatgagattc acttcaaaat tgattacaaa aacgcaagcg
     2581 gagcgatttc cagttattat cccgactttt tcgtaaagac ggacaataaa acgatttata
     2641 tcgtggaaac aaaaggacga gaagatttgg acgatgttga aaagataaaa agattggagc
     2701 aatggtgcga ggacgcaagc gaaagacaga aaaaaatcgc ctacaaaatg ctttatgtaa
     2761 agcaggaaga atgggataaa tataagccga cgagctttag gcagttggca gaagtgtttg
     2821 gtaataaggc ctaattatgg acgatataaa aactacaatt cacggggacg caacaagcga
     2881 ccaaattaaa gcaattgatc atgtcggtag ccatgctcga ttactcgcag gtcccggaac
     2941 tggcaaaaca aagacgctca cgcgccgtgt actttcttta atccttcaac acaatgccga
     3001 ccccgaaacg attcttttgc tgacatttac tcgtttggcg gctgctcaac tgaaagatga
     3061 gattaaaaag gtactggaac cgttaggcaa ggcaacgcca caagtttcaa cacttcattc
     3121 atttgcgtta aggcagattc tttttaattc aagcagggtt gacgctttgc cccgaccaat
     3181 aagaattgcc gatgattggg aagagcggca tattattcaa gaagatttga aaagaatatt
     3241 gaatcttaaa gaaattgccg atgttcaaaa actaataaac caactttcaa ccgattggga
     3301 aactttgaga gttgatgaaa ttggctggca acaacaattt ccaaaccccg cctttcttgg
     3361 agcgtggcaa ggccataaaa atcaatatgg cgaaacattg agagcagaac ttgtctatca
     3421 gcttaagagg caattaaatc aaaatagaga tttccagttg gacaaagaat ataaacacat
     3481 tttaatcgat gaatatcaag atttaaacgc ttgtgatttg gcagtcgtta atgaattggc
     3541 aaaacgagga gcagaattat ttgtcgctgg ggacgacgac caaagcattt atggcttccg
     3601 tttcgctact cctgacggta tcagaaattt tgatcagttt tatcaaaacg cccaaaagct
     3661 ggcgctagag atttgctttc gttgcgacaa aaatattctg cattcggctg aatttgttgc
     3721 cagtcttgat ccgcgccgtt tgccaaaacc gacacggaca aaagacgaag ccgacgacgg
     3781 tgaggtttta attatcggat ttagagatca ggtgcacgaa gcagaatcaa tagcaaagaa
     3841 aataaaaagt ttgattgacg ctggaacaaa tccagatcaa attacaatct tgcttagaag
     3901 cgaccgaaat ggcgcattat caaaaccgat tatagaaatg ttgaaaaaac aaaatgtcga
     3961 tgtttcgttg tcgaccgatt ccgagttaga taataacaat gagtatcgtg cggttttatc
     4021 aataattcgt ttgttggttg atcaaaatga ctgtttggct tggcgaactt tactacaaat
     4081 cggaaaaaat ggtttaggtg atgagtgtat aaaaaatata gaaaattacg ctcaaagcaa
     4141 ggatataaga tttagcgcag ctattcaaaa aattgccgaa aatcaaaatg aattgcgaaa
     4201 atttggtcag aaaatatcag cttttgtttc tggaacaaag acaattattg aagaattgaa
     4261 ggcggtcgaa aatccgattg aagtaatcaa tagtttggtt gataaattta tgaccgcaag
     4321 tgagataaaa gagcaggtga aaaaatattt tatcggaatt ttggagaatc aggaaaaaac
     4381 ggatttagaa aacttgatca aagcggtgag tatatcttct gaatttatcg aacaagaaac
     4441 caaagaaaat gccgtcaata ttttaacaat gcaccaagca aaaggcttaa cttttgatgt
     4501 ttgctttatc gtaggtgcag aagatgaatt tattcccgga cgaaacaccg gcgacaaaga
     4561 aggagatgaa agacggcttt tgtatgtatc aatgacgaga gcgcgccata aactttttat
     4621 cagttattgc aataagagaa ccggacagca gcgacattct ggaagtaata gcggaacgga
     4681 aaaaaggact ttgactagat ttcttaggga ttccaaaatt ccgataaaac aggtaaaata
     4741 aaaacaaata gggcggcgcg ccaacgaagt tggcacgacc tccagcgcca gcgaaaaaag
     4801 cgaaagcgga aaagagaggg cggggcggaa ttccccccag accccccttc cgccccgccc
     4861 gaaaaataaa attggcgtag cgaagcgttt ccgccgtctt ggtatcaaga catcaaagca
     4921 aaacaatttt
//
LOCUS       DAOQWE010000057         4876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_17653_length_4876_cov_8.582659, whole genome shotgun sequence.
ACCESSION   DAOQWE010000057 DAOQWE010000000
VERSION     DAOQWE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17653_length_4876_cov_8.582659"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="PKK37_02690"
     CDS             complement(<1..1700)
                     /locus_tag="PKK37_02690"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNR81318.1"
                     /translation="MDAKEIREKYLSYMKKRGHAIVPSCGLVPDNDPTTLFTSAGMQA
                     MIPYLSGQKHPLGSRIANSQKCLRTQDIEEAGDNRHTTFFEMLGNWSLNDYFKKEQLS
                     WMFEFLAKEVGLDPKNIYVTAFRGLPEAGIPKDEETANIWKELFVAAGVDAAAVDFAE
                     IGGMQNGRIFYYDEKKNWWSRSGTPDKMPEGELGGPDSEIFYDFGADLKLHENSPWKD
                     LPCHPNCDCGRFLEIGNNVFMEYVKRNGAVVKMENRNVDFGGGLERIAAASINDSDVF
                     KIDLFWPAIEKLQELSGKKYKDGTKTTAAMRVIADHIRAAVFLIGDGVFPSNKAQGYF
                     ARRLVRRAIVKGHELGIENNFLNEIASIYSNIYLNIYKLPAEQIKSELEKEETKFRKT
                     LKQGLKEMERRIAENASDGKQLFNLYQSYGFPLELSLEIAEEKNVSIAKSAIDDFNAE
                     LKKHQELSRTASAGMFKGGLADAGEQTTKLHTAAHLLLAALRRVLGDHVVQRGSNITA
                     ERLRFDFSHPRKMTLEQIKQVEDSVNEQIQKNIPVVRKEMPLSEAQQSGAMGVFGSKY
                     G"
     gene            complement(1711..2295)
                     /locus_tag="PKK37_02695"
     CDS             complement(1711..2295)
                     /locus_tag="PKK37_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR81319.1"
                     /translation="MPVLKLFSRSFGLVGGTAIALQIGHRRSIDFDMFSLDKFNNTRI
                     KNKILESTKIDEIFVNRLDELTFIARGVKFTFFRYIYEIPYSIKFGSGTRMPDLLTLA
                     AMKAFALGQRSKWKDYVDLYFIMRDFCGINEIVKKGEELFGGEFNDKLFAMQVGYFDD
                     IRYNEAVEFMPGFEVPEEEIKNKLVEYSMEWLKK"
     gene            complement(2327..2593)
                     /locus_tag="PKK37_02700"
     CDS             complement(2327..2593)
                     /locus_tag="PKK37_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81320.1"
                     /translation="MAEINEFIKKRPGLVWSTTQYDKLSEEVIVEAVLNYGNFKDVNE
                     IIRILGIKRVAAIFWEEASKKRSNLRPEVKHYFNLFFKKYVGNP"
     gene            complement(2658..3164)
                     /locus_tag="PKK37_02705"
     CDS             complement(2658..3164)
                     /locus_tag="PKK37_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81321.1"
                     /translation="MKKKIAVNDKMQRRYEYFLTEAAGENFAPDFLPELMPKQMLAMG
                     IFGGKYMTDCQNEFPADWYQDAKLNPNFHDPKLNYFGVNASQPLSVWRQKGWIHPADP
                     RGWFQWYCRYYIGRRMPEYDQWQIKRWKAMARHIAQIKKNCAPRDLSCRRRQRQAVLH
                     WAYDARTI"
     gene            complement(3161..4015)
                     /locus_tag="PKK37_02710"
     CDS             complement(3161..4015)
                     /locus_tag="PKK37_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81322.1"
                     /translation="MENIIIQNQEKFDEKLKAIVAGGIDKLHVRMDFDGTITKAIVDG
                     KIVPSLISVLRDEDFLGADYSQKAHALYNKYHAIEIDPVVSVADKKIAMRKWWETHFD
                     LLIEKGFSKCHIQRALQSARIRLRDGFGEFAAMLVKNKIPLVIMSCSGMGVDMIKMVL
                     EKNNVLFDNMRIVTNQYEWNDDGVAIAVKEPIVHTLNKDEESIRRIPDAYKTIKNRPN
                     VLLLTDSVADITMADGAKIGTLLSFGFLNSNIDKQLGAFEKNFDAVLLNDAPMNFPKD
                     ILKKVCGK"
     gene            complement(4028..>4876)
                     /locus_tag="PKK37_02715"
     CDS             complement(4028..>4876)
                     /locus_tag="PKK37_02715"
                     /inference="COORDINATES: protein motif:HMM:NF020127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1749 domain-containing protein"
                     /protein_id="HNR81323.1"
                     /translation="RLDGLLFAPEGEARAAVLHIAGRAGNFYANGFLDAMVENYTNAG
                     LALLSVNTRGHDHIADFRVGKTEKIVRMGQAFDIFEECVSDIATWVEFLKNKGFKKII
                     LQGHSQGAAKLVYFLSKESWPEIAAMVLMSPADAPGLLRKYQPEAFAQNLVLAKEMIR
                     SGRGEDLLPDKIRGWYYISAKTFVNEMSGKEADIFPIFDDGDFGKLENIKVPALAFYG
                     ENENTLIYSAQKDLEIIAPHLKNPRSKTFVINGADHTYLGKEEIAARMIVDWMENILF
                     EICKRN"
BASE COUNT         1278 a         1287 c          870 g         1441 t
ORIGIN      
        1 ccgtattttg atccaaagac ccccatcgca cccgattgct gtgcctcgga caatggcatt
       61 tcttttctaa ccaccggaat atttttttga atctgttcat tgactgaatc ctcaacctgc
      121 ttgatttgtt cgagcgtcat ttttcgcgga tgggaaaaat caaaccgcaa acgctcggcg
      181 gtaatgttgg agccgcgctg gaccacgtga tcgcccaaaa cccgccgcaa ggccgccagc
      241 agcaaatgcg ccgccgtgtg caatttggta gtttgttccc cggcgtccgc caaccccccc
      301 ttaaacattc ctgcgctggc ggtgcgggac aattcctgat gttttttaag ttccgcgttg
      361 aaatcatcta ttgcgctttt ggcaattgat acattctttt cttcggcaat ctccaagctt
      421 aattccaatg gaaatccata actttggtac aaattaaaca attgctttcc atccgatgcg
      481 ttttctgcaa tccgtctttc catttctttt aacccttgct ttaaagtttt cctgaatttg
      541 gtttcttctt tttccaattc acttttaatt tgttctgccg gcagtttgta tatatttaaa
      601 tatatatttg aatatatgct tgcaatctca ttcaaaaaat tattttcaat tcccaattca
      661 tggcctttga cgatcgcacg gcgcaccaaa cggcgcgcaa aatatccttg cgctttgttg
      721 gaaggaaaca cgccatcccc gatcaaaaac accgcggcgc ggatatgatc ggcgatcacg
      781 cgcatcgccg cggttgtctt tgtgccgtct ttgtattttt tgccggacaa ttcctgcaat
      841 ttttcgattg ccggccaaaa taaatctatt ttaaaaacat ccgagtcgtt gatactggcc
      901 gccgcgatgc gctccaaacc gccgccgaaa tcaacattgc ggttttccat tttaaccacg
      961 gcgccgttgc gttttacgta ttccataaac acgttattgc cgatttccaa aaaccggccg
     1021 caatcgcaat tgggatggca cggcaaatct ttccacggcg aattttcgtg caatttcaag
     1081 tctgcgccaa agtcataaaa gatctccgaa tccggtccgc ccaattcgcc ttcgggcatt
     1141 ttgtccggag taccggaccg cgaccaccaa ttcttttttt cgtcataata aaaaatcctt
     1201 ccgttttgca ttccgccgat ttccgcaaaa tcaaccgcgg cggcgtccac tcccgcggcg
     1261 acaaacagtt ccttccagat gttggcggtt tcttcatctt tgggaatccc ggcttcgggc
     1321 aaaccgcgaa acgcggtaac atagatattt ttcggatcca gaccaacctc tttggccaaa
     1381 aattcaaaca tccatgaaag ctgttctttt ttgaaataat cattcaacga ccagttgccc
     1441 agcatttcaa aaaaagtggt gtgccgattg tcccccgctt cctcaatatc ttgcgtgcgc
     1501 aagcattttt gcgaattggc aatgcgcgat cccagcggat gcttttgccc cgaaagataa
     1561 gggatcattg cctgcatccc cgcgctggtg aacaaagtcg tcggatcatt gtcgggcact
     1621 aaaccgcaag aaggcacgat cgcgtgcccc cgctttttca tataactcaa atatttctcc
     1681 cgaatttctt ttgcgtccat aatcatctct ttattttttt agccattcca tactatattc
     1741 aaccaattta ttttttattt cttcttcagg tacttcaaac cccggcataa attcaaccgc
     1801 ctcattgtat cttatgtcat caaaataacc aacctgcatt gcaaataact tgtcattaaa
     1861 ttcaccacca aaaagctctt cccccttttt aacaatttcg ttgatgccac aaaaatccct
     1921 cataataaaa tacaaatcga cataatcttt ccattttgat ctttggccta atgcaaacgc
     1981 tttcattgcc gccaaagtaa gtaaatccgg cattcttgtc cctgatccaa atttaatgga
     2041 ataaggaatt tcataaatat atctgaaaaa agtgaatttt actcctcttg cgataaaagt
     2101 gagctcatcc aaacgattta cgaaaatttc atctattttg gtactttcca aaattttgtt
     2161 tttgattcgg gtattgttga acttgtccaa agagaacata tcaaaatcaa tcgatctgcg
     2221 atggccaatt tgcaaagcaa ttgccgtgcc tcccaccagc ccaaaactcc gcgaaaataa
     2281 tttcaacacc ggcaaaagat tagtttgatt ttccgtgaga atttctttat ggattgccga
     2341 catatttttt gaaaaacaaa ttaaaataat gcttcacctc tggccgcaaa ttgcttcttt
     2401 ttttacttgc ttcttcccaa aaaattgccg ctactctctt gattcccaaa attcttataa
     2461 tttcgttcac atccttgaaa ttgccgtaat ttaaaaccgc ctccacgatc acttcctcgg
     2521 aaagtttatc atactgcgtg gtcgaccaca ccaatcccgg ccgttttttg atgaattcat
     2581 tgatttccgc catcaaattt tcgtaattta tggtctgatt ttaccacaaa tccgccccaa
     2641 aatccatttc tcacatatca gattgtgcgc gcatcatagg cccagtgcaa aacggcttgg
     2701 cgctggcggc ggcgacacga aagatcgcgc ggcgcgcaat tctttttgat ttgggcgata
     2761 tggcgggcca tcgctttcca gcgtttgatc tgccactggt catattcggg catgcgccgg
     2821 ccaatgtaat agcggcaata ccactgaaac cacccccgag gatccgccgg atgaatccat
     2881 cccttttggc gccagaccga cagtggctga gacgcgttaa ccccgaaata atttaatttg
     2941 ggatcgtgaa aattcggatt taatttggcg tcttgatacc aatccgccgg aaattcattt
     3001 tggcaatcgg tcatatactt gccgccgaat attcccatcg ccagcatctg ctttggcatc
     3061 aattccggca aaaaatccgg cgcgaaattc tcccccgccg cctcagtcaa aaaatactca
     3121 tacctcctct gcatcttatc attaacggca attttctttt tcatttgcca caaacctttt
     3181 ttaaaatatc ctttggaaaa ttcatgggcg cgtcgttcag taaaaccgcg tcaaagtttt
     3241 tttcaaacgc tcccaattgt ttatcaatgt tggaattcaa aaatccgaac gacaaaaggg
     3301 tgccaatttt tgcgccatca gccatggtaa tgtcggcaac cgaatcggtt aacagcaaaa
     3361 catttgggcg gttttttatc gtcttatatg catctggtat cctcctgatt gattcctcat
     3421 ctttattaag cgtatgaaca atcggctctt taaccgcgat tgccacacca tcatcgttcc
     3481 actcgtattg attggtgaca atacgcatgt tatcaaacaa aacattgttt ttctccaaaa
     3541 ccatcttgat catatccacc cccattccgc tgcacgacat tatcaccaac ggaattttat
     3601 tttttaccag cattgccgca aattcgccaa acccgtcacg caatcggatg cgcgcgcttt
     3661 gcagagcccg ctggatatgg cacttggaaa atcctttctc aatcaacaaa tcaaaatgtg
     3721 tttcccacca tttgcgcatc gcaatttttt tgtctgcgac agaaacgact ggatcaatct
     3781 caatcgcgtg atatttgtta taaagcgcgt gggctttttg cgaataatcc gcacccaaaa
     3841 aatcctcgtc acgcaaaacc gaaatcagcg acggcacaat tttgccatca acaatcgcct
     3901 tggtaattgt cccgtcaaaa tccatccgca catgcaattt atcaatcccg ccggcaacaa
     3961 tcgcttttaa tttttcatca aatttttctt ggttttgaat aataatattt tccataaaat
     4021 taaaaaatca atttcgtttg catatttcaa ataaaatatt ttccatccaa tcaacgatca
     4081 tccgcgcggc aatctcttcc ttacccaaat aggtgtggtc ggcgccattg atgacgaatg
     4141 tttttgatct tgggtttttc aaatgcggcg cgatgatttc caaatctttc tgcgccgaat
     4201 atattaaggt attctcgttc tctccataaa aggccaacgc cggaactttg atgttttcca
     4261 gcttgccgaa atcgccatcg tcaaaaatcg gaaaaatatc agcctctttc ccgctcatct
     4321 cattaacaaa agtcttggcc gagatataat accagccgcg gattttatcg ggcaaaagat
     4381 cttcccctct tcccgaccga atcatttctt ttgccaatac caagttttgt gcaaacgcct
     4441 caggttgata tttgcgcaac aaccccggcg cgtccgccgg cgacattaac accatggccg
     4501 caatttccgg ccaagactct ttactcaaaa aatagaccag cttggccgcg ccctggctgt
     4561 gtccctgcaa aattattttt ttgaatcctt tattttttaa aaattcaacc catgtggcga
     4621 tgtccgaaac acattcctca aaaatatcaa acgcttggcc catcctgacg attttctcgg
     4681 ttttgccaac ccgaaaatcg gcgatatgat catgcccgcg cgtgttcacc gacaagagcg
     4741 ccaagccggc atttgtgtaa ttttcaacca tggcgtccaa aaacccgttg gcgtaaaaat
     4801 tgccggcgcg tcccgcaata tgcaaaaccg cggcgcgtgc ctccccttcc ggcgcaaaca
     4861 gcaatccgtc cagccg
//
LOCUS       DAOQWE010000058         4843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_17787_length_4843_cov_8.319758, whole genome shotgun sequence.
ACCESSION   DAOQWE010000058 DAOQWE010000000
VERSION     DAOQWE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17787_length_4843_cov_8.319758"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKK37_02720"
     CDS             <1..308
                     /locus_tag="PKK37_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81324.1"
                     /translation="YLINKTEVAIPGKPLLAEEGIIGNLKKFTKKIEVVDANGICQKE
                     LGTAAVSGVYLLCLASFKNLIPLSPDSMREAIKSSVAAKHLEVNMKAWELAKKNAQA"
     gene            complement(451..1713)
                     /gene="murA"
                     /locus_tag="PKK37_02725"
     CDS             complement(451..1713)
                     /gene="murA"
                     /locus_tag="PKK37_02725"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNR81325.1"
                     /translation="MEKFIIKGGHPLKGQVQISGYKNSAGPILAAALLSDKPSVISNL
                     PQVSDVANLIEIIAQIGGEIEWLDRHTVKIGAKNLNPQKIPANIFQKMRISVLLIGPL
                     LARFRSFRVPHPGGDKIGLRPISTHLDAFEQFGVKIWRENGFYCFEAPKDIKPAHIVL
                     KEFSVTATENAMMLAAGIEGKSVIEIAAAEPQVQDLIMMLEKMGAKIIWTHPHTLEIQ
                     GTKNLGGANHEICPDPLETGTFMVAMAITGGQGKITGINPRHLTFFLEKMKEIGVDFA
                     IGENSITVNPSKNFKAARVQVMLYPGFPTDLQPQISVLLTQAAGKTLIHEPLYENRLR
                     HFEELRKMGADIEITDPHRALIFGKTELSGTTVDASDIRSGTALTLAALIAQGQTIVE
                     NISNIERGSEKFDEKLRRLGADIEKIGE"
     gene            1782..2771
                     /locus_tag="PKK37_02730"
     CDS             1782..2771
                     /locus_tag="PKK37_02730"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNR81326.1"
                     /translation="MTGLKNAKKKKIVCIGGGTQIPPLLLDNLRKRPVEIAGITSMVD
                     CGGSGGYFRREFGVLPASDIRRHILGLSNAPKWKKDLWQFRFGEEIFEDGHKGQVFAN
                     ALMAGLENSFKNYRKVLQYVFEFMELGKNRALPATIAQTHIVAELENGEIVKGEAEID
                     VPRRHDSKLKIKKVYLEPKAVAFPEAKKAILEADAIIIGPGDLYSSIVPCFLPAGISA
                     ALGKTRAKKILISNSLAKMGETQGFSVADFSAEIERYMRTKLDYVLYHNKPLDKALLA
                     AFQKECPQISDALKINGDLPPDKFVGRDIYQKDKIAFDSKKVVREIFKLINYK"
     gene            2789..4069
                     /locus_tag="PKK37_02735"
     CDS             2789..4069
                     /locus_tag="PKK37_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNR81327.1"
                     /translation="MTDKNNQLQAVILAAGESSRFWPLNSTHKSLLRIMGKPLIWYTI
                     DALRRIGVKDIVVVQGASRNVEEGLKEFNLEDIKYAIQQEPKGMGDAVMSARDHIIGQ
                     FFVLNAGRIDCQEIAAEMLDKSRQTGAKMVLAGQETDKTWLYGIARLEGDKVLEVMEK
                     PAEGNEPSKIKVTGVYLLDNKIFGYIGKVLGSANQNEEFEVAMSHYAKENDSRIIILD
                     KDYSSVSLKYPWHLFDAQKYLFDKFLTKKQIAKSAQIAKNATIEGNVHIGENCKIYEG
                     AVIKGPCYVGDNSVIGNNSVVRDNCNLEAGAMVGALCEAARTIFQPNVHIHSGYIGDS
                     ILDRGVRVGAGAITANVRLDRGQISARVKKEKDGIKVLSKVDTGLKSLGMIVGENTYL
                     GARVTILPARFIGKDCQIAAGAAVARNLDDNQKI"
     gene            4417..>4843
                     /locus_tag="PKK37_02740"
     CDS             4417..>4843
                     /locus_tag="PKK37_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNR81328.1"
                     /translation="MVKDKKLKINDFVIGVDGGGTKTAAALADLDGKIVARAVSGGSS
                     VRNFGIKLAAENVAKAIYDLIKRRKNIKITSVFIGLPAMEEEYRDKKPAIICELKKHK
                     KIAKIFEGKIAIGSDQLVAFRAGSYAKDGIVAICGTGSAV"
BASE COUNT         1323 a          983 c         1326 g         1211 t
ORIGIN      
        1 agtacctcat aaataagact gaagtggcta tcccgggcaa gccgttgctg gccgaagagg
       61 gcataatcgg caatttgaaa aagttcacca aaaaaattga agtggttgac gccaacggca
      121 tttgccaaaa agaattggga acggccgcgg tatcgggagt ttatttgctg tgtttggcgt
      181 cattcaaaaa tttgattccg ctgtcgccgg attcaatgcg ggaagccatc aaatcatcgg
      241 tggcggcaaa gcatttggaa gttaatatga aggcgtggga attagcgaaa aaaaacgcgc
      301 aagcgtgatt ttttgcattt gtaggggcga cctttatggt cgccctttgc aataaggaat
      361 gcgggcgggc acaaggccgc gcccctacaa acgcaaatgc aaaatccggc gcttgccgga
      421 tttttgatct taaatcttaa atcttggaat ttattcgccg atcttttcaa tgtcggcgcc
      481 cagcctacgg agtttttcgt caaacttttc cgaaccgcgt tcaatgtttg agatgttttc
      541 cacgatggtt tggccctggg cgatcagcgc cgccagggtg agcgcggtgc cggagcggat
      601 gtcggaagcg tccacggtgg tgccggacaa ttcggttttg ccgaagatca gcgcgcggtg
      661 cggatcggtg atttcaatgt cggcgcccat tttgcgcagt tcttcaaagt ggcgcaggcg
      721 attttcatag agcggttcgt ggatcaaagt ttttccggcg gcttgggtga gcaagaccga
      781 aatttgcggt tgcaggtcgg tggggaatcc cggatagagc atcacctgta ctcgcgccgc
      841 tttgaagttt ttggacggat taaccgtgat ggaattttcg ccgatggcaa aatcaacgcc
      901 aatctccttc attttttcca gaaaaaatgt cagatggcgc ggatttatgc cggtgatttt
      961 tccctggccg ccggtaatcg ccattgcgac cataaacgtg ccggtttcca gcgggtcggg
     1021 gcaaatttca tgattggcgc cgccaagatt ttttgttccc tggatttcaa gagtgtgcgg
     1081 gtgcgtccaa atgattttgg cgcccatctt ttccagcatc attatcaaat cctgcacttg
     1141 cggctccgcg gcggcgatct caatgaccga tttaccctca atgcccgccg ccagcatcat
     1201 cgcgttttcg gtggcggtga cggaaaattc tttcaaaacg atgtgcgcgg gtttgatatc
     1261 tttgggcgct tcaaagcaat agaatccgtt ttcgcgccaa attttgacgc cgaattgctc
     1321 aaacgcgtca agatgggtgg aaatggggcg caagccgatt ttgtcgccgc ccggatgagg
     1381 gactcggaaa ctccggaagc gcgccagcag agggccgatg agcagaaccg aaattcgcat
     1441 tttttggaaa atattggccg gaattttctg cggattgaga tttttggcgc cgatcttgac
     1501 ggtgtggcgg tcaagccatt caatttcgcc gccgatttgg gcgatgatct caatcaaatt
     1561 ggcgacatcg ctcacttgcg gcaaattgga aataacggac ggtttgtcgg aaagcaaagc
     1621 ggcggccaaa atcgggccgg cgctgttttt gtatcctgaa atttgcacct gccctttgag
     1681 cggatggccg cctttgataa taaatttttc cataaaagat tgaaattaag ctatttttgg
     1741 agtatcatac attctaagga ataaacggca ttttgtccat tatgaccgga ttgaagaacg
     1801 caaagaagaa aaaaatcgtt tgcatcggcg ggggaacgca gattccgccg ttgcttttgg
     1861 ataatttaag aaaacggccg gtggaaattg ccggcatcac atcaatggtt gattgcggcg
     1921 gttcgggcgg ttattttcgc cgcgagttcg gggttttgcc ggccagcgat atccggcgcc
     1981 atattttggg gctgtccaat gcgcccaaat ggaaaaagga tttgtggcaa ttccgttttg
     2041 gcgaagaaat atttgaagac ggccacaagg ggcaagtgtt tgccaacgcg ctaatggccg
     2101 gtttggaaaa ctcgtttaaa aattatcgca aggtcctgca atatgttttt gagtttatgg
     2161 agctggggaa aaaccgcgcg ttaccggcga ccatcgcgca aacgcatatt gtcgccgaat
     2221 tggaaaacgg cgagattgtg aagggtgagg ccgaaatcga tgtgccgcgc cgccatgatt
     2281 caaaattgaa aattaaaaaa gtttaccttg aaccgaaagc ggtggcgttt cccgaagcga
     2341 aaaaagcgat cttggaagcg gacgcgatta ttatcggccc gggagatttg tattccagca
     2401 tcgtgccatg ctttttgccg gccggaattt cggcggcatt gggaaaaacg cgagctaaaa
     2461 aaatattaat ttccaattcg ctggcaaaaa tgggagaaac ccaaggtttt tccgtggcgg
     2521 atttttctgc ggagattgaa agatatatgc gaacaaagtt ggattatgtt ctataccaca
     2581 ataagccgct tgataaagct ttgttggccg cgttccaaaa agaatgtccg caaatttccg
     2641 atgcgctgaa gattaatggc gatttgccgc cggataagtt tgtcgggcgc gatatttatc
     2701 aaaaagataa aatcgctttt gattccaaaa aagtcgtccg ggaaattttt aaactcatca
     2761 actataagtg ataagtgata aataataaat gacagataaa aacaatcaat tgcaggcagt
     2821 gatattggcc gcgggggagt cttcgcggtt ctggccgctt aattcaacgc acaaaagttt
     2881 gttgcggatt atgggtaagc cgctgatttg gtatacgatt gacgctttgc gtcggatcgg
     2941 cgtgaaagat attgtggtgg tgcagggggc gagccggaat gttgaagagg gattgaagga
     3001 atttaatcta gaagacatca aatacgcgat ccagcaagaa cccaaaggca tgggcgacgc
     3061 ggtaatgagc gcgcgcgatc atattattgg ccaatttttt gtgttgaacg ccggtcggat
     3121 tgattgccaa gaaatcgcgg ccgaaatgct ggacaaaagc cggcaaaccg gcgccaaaat
     3181 ggtgctggcc ggacaagaga ccgataaaac ctggctttat ggcatcgcgc gcttggaggg
     3241 cgacaaggtt ttggaagtga tggaaaaacc cgccgaaggc aatgaaccgt caaaaattaa
     3301 agtgacggga gtttatttgc tggacaataa aatttttgga tatattggaa aagttttggg
     3361 aagcgccaac caaaacgaag aatttgaagt ggcaatgtcg cattatgcca aagaaaatga
     3421 ttcgcgaatt ataattttag ataaagatta ttccagtgtt tcattgaaat acccgtggca
     3481 tttgtttgat gcgcaaaaat atctttttga caaattttta acaaaaaaac aaattgccaa
     3541 atcggcgcag attgccaaaa acgcgaccat tgagggtaat gtccatattg gcgaaaattg
     3601 caaaatttac gaaggcgcgg tgataaaggg cccgtgctat gtcggcgaca attccgttat
     3661 cggcaacaac agcgtggtgc gcgacaattg caatttggaa gcgggcgcga tggtaggcgc
     3721 gctgtgcgaa gcggcgcgca cgattttcca gcccaatgtc catatccatt cgggatatat
     3781 cggcgattca atcctggatc ggggagtgcg ggtcggcgcg ggcgcgatca cggccaatgt
     3841 gcggttggat cgggggcaaa tatccgcgcg ggtgaaaaaa gaaaaagacg gcatcaaagt
     3901 gctgtccaaa gttgataccg gtttgaaatc gctggggatg atcgtgggcg agaacacgta
     3961 tcttggcgcc cgcgtcacca ttctgccggc ccgtttcatc ggcaaagact gccaaatcgc
     4021 cgccggcgcg gcggtggcgc gcaaccttga cgacaaccaa aaaatttgac ttttgggaca
     4081 gaatatgcta cgcagatagt gtaaaacaaa atcaaaccac ggagataatc cgtgagttgt
     4141 gtttttgttt tggcagagga gatttaaatg gcactattaa aaataacatc ggaacaattg
     4201 gagagcttaa agaaagtttt aaatgagcaa aaaatacctt tcaaaattat tggaagcaaa
     4261 gggttgcatt taataattca aatttgaata aagtatttaa tgctccatag cctggcgcgt
     4321 cgcgcttggc tcttttttat atagtgaggc gtggtgtggt agaatgatag taaattcaat
     4381 tatcaatgat aaattatcaa ttataaaaat gataaaatgg ttaaagataa aaaattaaag
     4441 attaatgatt ttgtcatcgg agtggacggg ggaggaacca aaactgccgc ggctttggcg
     4501 gatttggacg gcaagattgt ggcgcgggcg gtttcgggcg ggtcaagcgt gcgcaatttt
     4561 ggcataaaat tggcggcgga aaatgttgcc aaggcgatat acgatctgat aaagcggcga
     4621 aaaaatatta aaatcacctc cgtttttatc ggcttgccag cgatggaaga agaatatagg
     4681 gataaaaagc cggcgataat ttgcgagctg aaaaaacaca aaaagattgc caaaattttt
     4741 gagggcaaga ttgccatcgg gtcggaccag ttggtggcgt ttcgcgcggg cagttatgcc
     4801 aaagacggta tcgtggccat ttgcggcacc gggagcgccg ttc
//
LOCUS       DAOQWE010000059         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_18772_length_4656_cov_6.540317, whole genome shotgun sequence.
ACCESSION   DAOQWE010000059 DAOQWE010000000
VERSION     DAOQWE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18772_length_4656_cov_6.540317"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1355)
                     /gene="murC"
                     /locus_tag="PKK37_02745"
     CDS             complement(24..1355)
                     /gene="murC"
                     /locus_tag="PKK37_02745"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNR81329.1"
                     /translation="MKKVFFIGIGGIGVSALARYYLSQGWEVFGSDLARSEITDDLKK
                     IGARIFVKKNINLKKTAPDLAIYSLAAKNNPELRRVQDMGVKIMSYPQALGELTKKYR
                     TVAVAGAHGKSTVTAMIGMMLAAAKFDPTVVVGTKVKSFKNTNFRSGKSDLLVIEACE
                     YDRAFWNYFPDIAVVTNIEMDHIECYENEANLRAAFEKFAGNLKPGGKLLVCADDAGA
                     KKLGESMKKKGIEVLNYSRTMPEAKFFSEILRVPGEHNVTNALAALAVGRMLEIDDET
                     IFKSLAKYRGSWRRFDRRRGEIFGKKITVVFDYGHHPTQIKLTMAAARQLWPKKRIIC
                     VFQPHQAWRTSLLFDDFAKVFRESPLDKTFITDIYRVAGRENEKISKDINSEKLADAV
                     GSKKTEYLAKKQIAVKLKKEINKGDILLVLGAGDIYDYMQKILRNQSCDCA"
     gene            complement(1472..2089)
                     /gene="thpR"
                     /locus_tag="PKK37_02750"
     CDS             complement(1472..2089)
                     /gene="thpR"
                     /locus_tag="PKK37_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNR81330.1"
                     /translation="MRNLHRIFIAVNLPENLRRYLAEFSRKWPELPAKWAKPENLHVT
                     LSFLGNTNDEELSEICQSAIKVAQRHDPFVLGLDCIVYGPQNEFPRRQKEGDISRYPR
                     MVWAAGEKSQEIGNLQKDLESSLFEFSGARYRKDDSDYAFRPHVTLARLIQTDLRRMD
                     PEEIPTIEEKIAKTFTAESIEVMESELRRGGPIYTVLESAKLGQQ"
     gene            2366..2677
                     /locus_tag="PKK37_02755"
     CDS             2366..2677
                     /locus_tag="PKK37_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81331.1"
                     /translation="MKEHINGKTRDTVYNLLRDLGYHFQGQDEKTKELGFARPKTGYP
                     RLHLFIKIENDGLTLSLHLDQKKPVYDGATAHAGDYDGPVVEREMARIKQYIESQTDK
                     S"
     gene            2681..3271
                     /locus_tag="PKK37_02760"
     CDS             2681..3271
                     /locus_tag="PKK37_02760"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNR81332.1"
                     /translation="MEVMLLTKDNFQAAVEAAATVIYRGGTVIAPTDTVYGLLANAAN
                     EKAIEKIFAAKLRPKNKPLPIFVKNMAMAKKLAKISPAQEKFLEEKWPGKFTFRLNRK
                     KEVKLFGIEENTVALRIPFHSFVNSLLEATNSPLCGTSANISGRPASGKIDAVLAQFR
                     GNAVFPDLVINAGDLDKPSPSTIIDLTQIPPQTIRP"
     gene            3285..4004
                     /locus_tag="PKK37_02765"
     CDS             3285..4004
                     /locus_tag="PKK37_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNR81333.1"
                     /translation="MEKKAKKIKPTINKKQELELLREIIETISYNLDLKEVLGRIVKI
                     VSGVTKADSCFLYLLDGNNLVLRASLNPKPMEIGNIKLKTGEGITGWVARNKQTAAIA
                     KEAYNDERFKTVNSLPEDKFEGFLSVPIVYHDKVLGVINVQHSKPRAYSKTEIALFES
                     VAKAVGGAIENASLFSEKEALKEALETRKIIEKAKGILMKEFAVGEDDAYKLLHKKAM
                     DKRLPMKEIANAIIISSEFKK"
     gene            complement(4067..>4656)
                     /locus_tag="PKK37_02770"
     CDS             complement(4067..>4656)
                     /locus_tag="PKK37_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81334.1"
                     /translation="VGNWWPWEKVSDINFNALRSQVNSPYKAYNLNNSAALGKCVPEG
                     QTKTFTFDVKAPATPGTYNFQWQMVQDGVEWLGDKTPNVVVAVVSSAGCTPKTCAALG
                     NYQCGSWSDGCGAMVSCGTCTSGKTCNASGRCVSQTAGGPVISDSDPQTLTETPQKMT
                     RAEVLQKIAEVKQLLIQLIIQLIAELQKQLAAMPK"
BASE COUNT         1306 a         1134 c          992 g         1224 t
ORIGIN      
        1 atttccgccg gggcaaattt ctttcacgcg caatcgcacg attgattgcg taaaattttc
       61 tgcatatagt cataaatatc gccggcgccc aacaccagca aaatgtcccc cttgttaatt
      121 tcttttttca gcttgaccgc gatttgtttt ttggcaagat attccgtctt ttttgaacca
      181 acggcatcgg ccaatttctc actgtttatg tctttggaaa ttttttcgtt ttcccggccg
      241 gcaacccggt aaatatcggt gataaacgtt ttatccagcg gcgattcgcg gaaaactttg
      301 gcaaaatcat caaaaagcaa actcgtgcgc cacgcttggt gcggctgaaa aacgcaaatg
      361 atcctttttt tgggccacaa ttgacgcgcc gccgccatcg tgagttttat ttgcgtggga
      421 tggtggccgt agtcaaacac cacggtaatt tttttgccga aaatttctcc ccggcggcga
      481 tcaaagcggc gccacgaacc acggtatttt gccaaagatt taaaaatcgt ttcgtcatca
      541 atttccaaca ttcgtccaac ggcgagcgcc gccagcgcat tggtgacgtt atgctctccc
      601 ggaacacgca aaatttccga aaaaaatttc gcctcgggca ttgttcgcga ataatttaaa
      661 acttcaatcc cctttttctt cattgattcg cctaactttt tggcaccggc atcgtcggcg
      721 caaaccagca atttcccgcc gggcttcaag ttgccggcaa atttttcaaa cgccgcgcgt
      781 aaattcgctt cgttttcgta gcactcaatg tgatccattt cgatgttggt caccacggca
      841 atatcgggga aataattcca aaacgcccgg tcatattcgc aagcttcaat caccaacaga
      901 tcggattttc cggatctgaa attggtattt ttaaaacttt taactttcgt gcccaccacc
      961 accgtgggat caaattttgc cgcggccaac atcataccga tcatcgccgt caccgtgctt
     1021 ttcccgtgcg caccggcgac agcaaccgtc cggtattttt ttgttaactc tcccaatgct
     1081 tgcggataac tcattatttt aacccccata tcttgcaccc gtcttaactc cggattgttt
     1141 ttcgccgcta aactataaat tgccaaatcc ggcgcggttt tttttaaatt gatatttttt
     1201 tttacaaaaa tacgcgcacc gatttttttc aaatcatcgg tgatttcgga tcttgccaaa
     1261 tcggaaccga aaacttccca cccttgcgac agataatatc gcgccagcgc cgaaactccg
     1321 attccgccaa taccgatgaa aaaaactttt ttcataaatt ccaaatatca agcattaaat
     1381 cacaaagtaa attccaatgt caaaaattta aattttaaat attgagactt gaatattgct
     1441 tggagcttgg gatttggttc ttgtaaatta attattgttg gccgagcttt gcgctttcca
     1501 gcacggtgta aatcgggccg ccgcggcgca attcgctttc cattacctca attgattcgg
     1561 cggtaaatgt tttggcaatt ttttcttcaa ttgttggaat ttcctcggga tccatgcggc
     1621 gcaaatcggt ttgaatcagc cgcgccaaag tcacatgggg acgaaaagca taatcggaat
     1681 cgtcctttcg gtaacgggcg ccggaaaact caaacaagga gctttccaga tctttttgga
     1741 ggttgccgat ttcttgcgac ttctctcccg ccgcccaaac catacgcgga tatcgtgaaa
     1801 tgtccccttc tttttgccgg cggggaaatt cgttttgcgg cccgtagaca atgcaatcca
     1861 atcccaaaac aaacggatcg tggcgctgtg cgaccttaat tgcagattgg caaatttcgc
     1921 ttaattcttc gtcgttagta tttcccaaaa agctcaaagt gacgtgcaaa ttttctggct
     1981 tggcccattt tgccggcaat tccggccatt tccgggaaaa ctccgccaaa taacggcgca
     2041 aattttccgg caagttaacg gcaataaaaa ttcggtgcaa attgcgcata ataagattga
     2101 atcacaacct cattttaaac catttggcaa aaaaagaaag atggagtaaa atcccgatta
     2161 gattccccta aattacaaaa caaatatcgc cagagaagta tcgctatttg atatcgctaa
     2221 attttagcga tatcaaatag cgatatcaaa taccgctatg atatatttga ggattgattt
     2281 gatttgttga ttttcggtga tgaacatatc aacgaatatg cctgcgaata ctattcgctt
     2341 atcaattatt taataacgaa tatatatgaa ggagcacatt aacggaaaaa ccagggatac
     2401 cgtctataac ttactgcgcg atttgggata tcattttcag ggacaagacg aaaaaaccaa
     2461 agaattgggt tttgcgcgtc ccaaaaccgg ctatccccgc ttgcatttgt tcataaaaat
     2521 agaaaatgac ggattgactc tcagtttgca tctggatcaa aagaaaccgg tttatgatgg
     2581 cgccaccgct catgccggag attacgacgg tccggtggtg gaacgagaaa tggccagaat
     2641 aaaacaatac attgaaagcc aaaccgataa aagttaatct atggaagtaa tgttgctgac
     2701 aaaagacaat tttcaggcgg cagtggaggc ggcggcaacg gtaatctatc gtggcggcac
     2761 agtgattgcg cccaccgaca cggtctacgg cttgctggcc aacgccgcaa acgaaaaagc
     2821 gattgaaaaa atttttgccg cgaaattaag gccaaaaaac aaacctctgc cgatatttgt
     2881 gaagaatatg gcaatggcca aaaaattggc aaaaatttct ccggcacagg aaaaattttt
     2941 agaagaaaaa tggccgggaa aattcacctt ccggctgaat cgcaaaaaag aagtgaagct
     3001 tttcggcatt gaagaaaaca ctgtcgccct ccgcatcccc ttccattcat tcgttaattc
     3061 ccttttggag gccacaaatt cgcccctctg cggcacttcg gccaatattt ccggccggcc
     3121 ggcatccggg aaaattgacg cggttctggc tcaatttcgc ggcaacgcag tttttccgga
     3181 tctggtcatc aacgccggcg atctggacaa accaagtccc tcaaccatca ttgacttgac
     3241 ccaaatccca ccgcaaacta tccgtcccta aaaaaaatac gcttatggag aaaaaagcaa
     3301 aaaaaataaa accgaccatc aacaaaaaac aagagttgga gcttttgcgc gaaatcattg
     3361 agaccatcag ctacaatttg gatttaaaag aagttttggg ccgcatcgtc aaaatcgtga
     3421 gcggcgtcac caaggcggat tcttgttttt tgtatttgct ggacggcaac aatctggttt
     3481 tgcgcgcgtc gttgaatccc aaacccatgg aaatcggcaa cataaaattg aaaaccggtg
     3541 aaggaatcac cggctgggtg gcgcgcaaca aacagaccgc ggccatcgcc aaggaagctt
     3601 ataacgacga aaggtttaaa accgtcaaca gcctgcccga agataaattt gagggatttc
     3661 tttccgtgcc gattgtttat catgacaaag tgctgggggt gattaatgtc cagcactcaa
     3721 aaccgcgggc atactccaaa accgagattg cactgtttga atcggtcgcc aaagccgtcg
     3781 gcggcgcgat tgaaaacgcg tccctctttt cagaaaagga ggcgttaaaa gaggcactgg
     3841 aaaccagaaa aatcattgaa aaagcgaagg gtattttgat gaaagaattc gccgtcggcg
     3901 aagacgacgc ctataagctt ctgcacaaaa aagcgatgga caaacgcctg ccgatgaaag
     3961 aaatcgccaa cgccattatc atctcctcgg aattcaaaaa atagtttgca accattctat
     4021 aaaaaatccg ccaaaaagcg gatttttgtt cccgataagt ccttggttat tttggcattg
     4081 ccgccagctg tttttgcagt tcggcgatga gctggatgat gagctgaatt aacagttgtt
     4141 tgacttcggc gatcttttgc agtacctcgg cgcgagtcat tttctgcggc gtttcggtca
     4201 gggtctgcgg atcggaatcg gaaattaccg gcccgccagc cgtctggctc acgcaccgcc
     4261 cggaagcatt gcaagttttg ccgctggtac aagtgccgca actgaccatt gctccacacc
     4321 catcgctcca cgatccgcac tggtagtttc ccagcgcggc acaagtttta ggagtgcaac
     4381 ctgccgaaga gacaactgcc accaccacat tcggagtttt gtcgccaagc cactccacgc
     4441 cgtcctgcac catctgccat tggaaattgt aagtgccggg agttgccggc gccttcacgt
     4501 caaaagtgaa ggtttttgtc tggccttccg ggacacattt accaagtgcg gccgaattat
     4561 ttaaattata agctttataa ggagaattga cttgcgatct aagtgcattg aaattaatat
     4621 cacttacttt ttcccaaggc caccaattgc caacct
//
LOCUS       DAOQWE010000060         4609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_19029_length_4609_cov_11.149539, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000060 DAOQWE010000000
VERSION     DAOQWE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19029_length_4609_cov_11.149539"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..438)
                     /locus_tag="PKK37_02775"
     CDS             complement(55..438)
                     /locus_tag="PKK37_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNR81335.1"
                     /translation="MEFSFTGGGKEYQIKIEDCGERGVRVAVNGKEFCFGADAAVEAA
                     PVAQAAMPKRSLGAKEVAAPLAGTISEIAVGAGDIVSAGQKLLTLSAMKMENEILSEI
                     DGKIKEIKIAQGQKVAEGDILIILV"
     gene            complement(579..2123)
                     /locus_tag="PKK37_02780"
     CDS             complement(579..2123)
                     /locus_tag="PKK37_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNR81336.1"
                     /translation="MVEIKKILIANRGEIALRIIRTCREMGIKTVALCPRPGMEKNFL
                     ETSLADEFYYLENDGPAGYLDKEKIIALAKGAKADAIHPGYGFLAENWRFSLMCEKSR
                     IKFIGPNWRTLRAFEDKIEAKKIAKKIGAPTVPASDSSIKSKKDLIKWAEKIKPPFII
                     KAQRGGGGMGIRVVNGQMNYEEMMALSLGAQRQIAGAFDDVDFFLEKYLPRVKHIEFQ
                     ILGDGKKAVHLGERECSIQRRFQKLVEESPSSALDADLRAKMGAWAVKIAEHLRYEGA
                     ATVEFLLDDDKNFYFMEVNPRIQVEHPVTEAVTGIDIVEQQIKIAQGEKLSFGQDSVY
                     QNGWAIEVRVNAEDPHKNFVPAPGTVAKYIPPQGQGVFVHSFLQDGQEIYPFFDSLLA
                     KVIAYGKDRPAAITKLKRALSETVIDGVATTIPFFQALLEKEEFARGDFYTNYIEKSG
                     IIREMMLKPYLKKKIVCEPKDFGEEAVAQIAHKIYQEMKNVAGGTQNGNLSRWVLADR
                     ASEIEN"
     gene            complement(2229..2897)
                     /locus_tag="PKK37_02785"
     CDS             complement(2229..2897)
                     /locus_tag="PKK37_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR81337.1"
                     /translation="MNSFVSTISKMILGKPIEERMIKETSGKENIPTGGFILASNHLS
                     HADWFVSGYLCAPKKFVFIGQTDQYNTGVNAIWRRLIYWWGSTIPIDRKSDESKKRAI
                     AKAVAMLKKGYRVIIYPEGGRAYDGIMREFKPGVGMLHLESGIPVLPAAFSGTREILP
                     PHGKIKIKKIVRINIGRPLDFSKERAIAAKLDKHSRAYHGLCADVSKKIEEEVRRLAG
                     QIKN"
     gene            complement(3013..3675)
                     /locus_tag="PKK37_02790"
     CDS             complement(3013..3675)
                     /locus_tag="PKK37_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNR81338.1"
                     /translation="MTRPVSFISWILMGWFFELFLIKEIRGKENFSKKRNYILASNHV
                     SHLDWLIGCYLCVPRKYTFIGQVDKIKGIAGALRDLMYWTGETIRVNRNDPASRVRAA
                     AKAVEFVKKGYNLFMFPEGTRSRDGLLKTFKRGVGKIYLQTGAPILPVAMVGTYEMMP
                     PGEKMKLNRSARIIIGKPREFPKELAAAKNMNPESEAYLRLCETIAKKAEEEVRELLA
                     QN"
     gene            complement(3678..>4609)
                     /locus_tag="PKK37_02795"
     CDS             complement(3678..>4609)
                     /locus_tag="PKK37_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNR81339.1"
                     /translation="NKLTKLLNIKYPIIQGGMAGVSEAVLTAAVSNAGGMGVIGSGFA
                     SSEWLAEEIKKTKALTDKPFGVNLLLQNPGAPELVKVIIKEKVAAVWTGGGNPLPVLP
                     HFKRAGVKVIPVVPYVRLALKMEQAGADAVAVEGLESGGHIGESTTMCLVPQAAAAVK
                     NIPVIAAGGICDGRTMAAALILGASGVQIGTRFLAATECQIHESYKEAVIKATDEDIA
                     VVARFTGHPVRMIKNKFVESVRKIEAKNPFPEEIKSERFAGNKGGANADLSALLCGTC
                     AGSVKEIKTCQEIIDEIVVGAKEILDNAAALLQ"
BASE COUNT         1063 a         1287 c         1022 g         1237 t
ORIGIN      
        1 tatttttatt ttaaaacctc acttaaacag tgagctacgg atattgacaa aatgctatac
       61 caaaataata aggatgtcgc cttcggcgac tttttggcct tgggcgattt tgatttcttt
      121 gattttgccg tcaatttcgg aaaggatttc gttttccatt ttcatcgccg acagagtcaa
      181 aagtttttgc cccgccgaaa cgatgtcgcc ggcgccaacg gcgatttccg aaatggtgcc
      241 ggccagcggc gcggcgactt ctttggcgcc cagcgaacgc ttgggcatcg ccgcctgcgc
      301 cacgggcgcg gcctcaaccg cggcatccgc gccaaaacaa aactccttgc cgttgacggc
      361 aacccgcact ccccgctcgc cgcaatcctc aattttgatt tgatactcct tgcctccgcc
      421 ggtaaatgaa aattccatgt tgaaaattca aaattaaaaa atcaaaaata aaaatgacaa
      481 tataaaatta aaaattatgc gaccagggcg cacccgcctt gagcggtata aatcaaaatt
      541 ccgttgggaa ttttacctta atttttcatt ttgatttttc aattttcaat ttcgcttgct
      601 cggtccgcca gcacccaccg ggaaaggttg ccgttttgcg tcccgccggc gacatttttc
      661 atttcttgat aaattttgtg ggcaatttgc gccacggctt cttcgccgaa atctttgggc
      721 tcgcaaacaa tcttcttttt cagatagggc tttaacatca tttcgcggat gatgccgctc
      781 ttttcaatgt aattggtgta aaaatcgccg cgggcaaatt cttctttttc cagcaacgct
      841 tgaaaaaacg gaatcgtggt cgccacgccg tcaatcacgg tttcggaaag cgcgcgcttg
      901 agtttggtga tcgccgccgg ccggtccttg ccataggcaa tcactttggc cagcaaagag
      961 tcaaaaaacg gatagatttc ctggccgtcc tgcaaaaaac tgtgcacaaa caccccctgc
     1021 ccctgcggcg gaatgtattt tgccaccgtt cccggcgccg gcacaaaatt tttgtgcgga
     1081 tcctcagcgt tgacgcgcac ttcaatcgcc cagccgttct ggtaaacgct gtcttgccca
     1141 aacgataatt tttccccctg ggcgatcttg atctgctgtt ccacgatatc aatgccggtg
     1201 accgcttcgg taaccggatg ctccacctga atgcggggat tgacttccat aaaatagaaa
     1261 tttttgtcgt cgtccagcaa aaattccacc gttgccgcgc cctcatagcg cagatgctcg
     1321 gcgatcttga ccgcccacgc gcccattttg gcgcgcaaat cggcatccag cgccgaagag
     1381 ggcgattctt ccaccagttt ttggaaacgg cgctggatgg aacattcgcg ctcccccaaa
     1441 tgcaccgcct ttttgccgtc gcccaaaatt tggaattcaa tgtgctttac ccgcggcaga
     1501 tatttttcca aaaagaaatc aacatcgtca aacgcgccgg cgatctgccg ctgggcgccc
     1561 aaagacagcg ccatcatttc ttcatagttc atctgtccgt tgaccacgcg gatgcccatt
     1621 ccgccgccgc cgcgctgagc cttgatgata aacggcggtt ttattttctc cgcccatttg
     1681 atcaaatctt ttttgctttt gatggaacta tccgacgccg gcaccgtggg cgcgccgatc
     1741 tttttggcga tttttttggc ctcaatcttg tcttcaaacg cgcgcaatgt ccgccaattc
     1801 ggcccgataa acttaatccg gcttttttca cacatcaaag aaaaccgcca attttcggcc
     1861 aaaaaaccgt aacccggatg gatcgcgtcg gctttcgctc ccttggcgag agcgatgatt
     1921 ttttctttgt ccaaataccc cgccggcccg tcattttcca aataataaaa ctcgtcggca
     1981 agggacgttt ccaaaaagtt tttttccatt cccgggcgcg ggcacagcgc cacggttttg
     2041 atgcccattt cgcggcaagt gcggataatc cgcagagcga tctctccccg gttggcaatc
     2101 aatatcttct taatttccac cataaaattt aaaaactaag aatgaaaaat taaaaacgaa
     2161 agttaaaagt ttaaaattat cgttttttcg gtttttaatt ttagattgtc gtttttactt
     2221 tttagatctc aatttttaat ttggccggcg aggcggcgaa cttcttcttc tatctttttt
     2281 gacacgtcgg cgcaaagccc gtgataggcc cgggaatgct tatccaattt ggcggcaatt
     2341 gcacgctctt tggaaaaatc cagcggccgg ccgatattga tgcggacgat ttttttgatc
     2401 tttattttcc cgtgcggcgg caaaatttcg cgcgtgcccg aaaacgccgc cggcagaacg
     2461 ggaatcccgc tttccaaatg caacatcccc acgccgggct tgaattcgcg cattatgccg
     2521 tcataggcgc gaccgccctc gggatagata atcacgcgat agcctttttt gagcatcgcc
     2581 accgccttgg caatcgcccg tttttttgat tcgtcgcttt tgcggtcaat gggaatggtg
     2641 ctgccccacc aataaatcaa ccggcgccaa atcgcattga cgccagtgtt gtattgatcg
     2701 gtctgcccta tgaaaacaaa tttcttgggc gcgcaaagat aaccgctcac gaaccagtcg
     2761 gcatggctca aatgattgga cgccaaaatg aacccgcccg ttggaatgtt ctctttcccg
     2821 gaggtttctt taatcattcg ttcctcaatc ggcttgccca aaatcatttt tgaaattgtg
     2881 gaaacaaaag aattcatttt tcaatttaaa aactaagaat gaaaaattaa aaacgaaagt
     2941 taaaagttta aaattatcgt tttttcggtt tttaatttta gattgtcgtt tttacttttt
     3001 agatctcaat ttttaatttt gcgcgagcaa ctcgcggact tcttcttccg cttttttggc
     3061 gatggtttca caaaggcgca aatacgcttc ggattcggga ttcatatttt tcgccgccgc
     3121 cagttctttg ggaaattccc gcggtttgcc gatgatgatt ctggccgaac ggttcaattt
     3181 cattttttcc cccggcggca tcatttcata ggtgccgacc atcgccaccg gcaagattgg
     3241 cgcgccggtt tgcagataaa tcttacccac gccccgcttg aaagttttca gcaagccgtc
     3301 ccgcgaacgc gtcccctcgg gaaacataaa caaattatag ccttttttga caaattcaac
     3361 cgctttggcc gcggcgcgga cgcgcgaggc gggatcgttg cgattaacgc ggatcgtttc
     3421 ccccgtccag tacatcaaat cgcgcaacgc gccggcaatc cccttgatct tgtccacctg
     3481 accgataaaa gtgtattttc ggggaacgca cagataacag ccgatcagcc aatccaaatg
     3541 actcacatgg tttgacgcca aaatgtaatt cctttttttg gaaaaattct ctttgccccg
     3601 gatttcttta atcaaaaaca gctcaaagaa ccatcccatc aatatccaag aaataaacga
     3661 caccgggcgc gtcatagtta ttgcagtaac gccgcggcat tatccaaaat ttctttggcg
     3721 ccgacgacga tttcgtcaat gatctcttgg caggttttga tttctttgac gctccccgcg
     3781 caggtaccgc acagcaacgc cgacagatcg gcgttggcgc cgcctttgtt gccggcaaaa
     3841 cgctccgatt tgatctcttc gggaaaagga tttttggctt caatcttgcg caccgattcc
     3901 acgaatttgt ttttgatcat ccgcaccgga tggccggtga agcgcgccac caccgcgata
     3961 tcctcatcgg tcgccttgat caccgcctcc ttgtagcttt cgtgtatctg gcattcggtg
     4021 gcggctaaaa atcgcgtgcc gatctgcacg ccggaagcgc ccaaaatcag cgcggcggcc
     4081 attgtccggc catcgcaaat gccgccggcg gcaatcactg ggatgttttt gaccgccgcg
     4141 gccgcttgcg gcaccaggca cattgtggtg ctctccccga tgtgtcctcc cgactcaagc
     4201 ccctccaccg ccaccgcatc ggcgccggct tgctccattt tcaacgccaa gcgcacgtaa
     4261 gggaccaccg ggatcacctt cacgcccgcc cgcttaaaat gcggcagtac cggcagagga
     4321 ttgccgccgc cggtccacac cgccgcaact ttttctttga taatcacttt taccaattcc
     4381 ggcgcccccg gattttgcaa tagaagattc acgccaaacg gcttgtccgt gagcgctttg
     4441 gttttcttga tttcttcggc cagccattcc gaagacgcga atcccgaacc gatcacgccc
     4501 atcccgccgg cattggaaac cgccgcagtc aacaccgctt ccgacactcc ggccatcccg
     4561 ccctggatga tgggatactt gatatttaaa agtttggtga gtttgttat
//
LOCUS       DAOQWE010000061         4602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_19067_length_4602_cov_7.644381, whole genome shotgun sequence.
ACCESSION   DAOQWE010000061 DAOQWE010000000
VERSION     DAOQWE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19067_length_4602_cov_7.644381"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..2778
                     /gene="leuS"
                     /locus_tag="PKK37_02800"
     CDS             238..2778
                     /gene="leuS"
                     /locus_tag="PKK37_02800"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNR81340.1"
                     /translation="MENQKNKKEYQPQMIEPKWQKIWEESNLYRAQDFSDKPKAYLLV
                     EFPYPSGEGLHVGHVRSYSAMDCVARKRRMEGENVLFPIGWDAFGLPAENYAIKTGIH
                     PSITTQKNIANYTRQLKSMGLSFDWSRQVDTTDSGYYKWTQWIFLKLYERGMAYQAEI
                     PVNWCPKCKTGLANEEVIAGNCERCGESVTRKTLKQWMLAITKYADRLIDDLSRVDFW
                     EKIKIQQKEWIGKSEGATIDFAIADSAERVSIFTTRTDTIFGVTALVLAPEHELVAKL
                     KGQIKNWNEVCAYIGATNKKSEFERLNLEKKRTGICLEGVLAINPANNEKIPVWLGDY
                     VIAAYGGGAVMMVPAHDARDYDFARRYQIKSILVVQPRSKNDITTYNTHPSFAPEILE
                     GESVDDATERNRMQEYNLVLNGKRCYTGGGLLVNSGEFNGLGSETAIEKICEWLQEKG
                     AAKKTVHYKLRDWIFSRQHYWGEPIPIIHCEKCGIVPVPENELPVKLPHIEKYQPTGT
                     GESPLAVIDEWVNVKCPKCGGAAKRETDTMPNWAGSNWYYVRYCDPYNERQIGAEDKL
                     DYWLPVDWYNGGMEHTTLHLLYSRFIYKFLFDIGAVPTAEPYQKRTSQGMVLAANNQK
                     MSKSRGNVVNPDDIVKEYGADTLRIYELFMGPFDQAIAWSADAVRGSRKFLGRVWNLV
                     FECANNENSGEDVLRRVHKLNQKISGDIEQLKFNTAIAAFMEFLNFAEDNKSDVGKNV
                     LQRLVMLLAPFAPHIGEELWQKLDNNESVFKQPWPQPDKNLVKDETIELLVQVNGRLR
                     DKIAVPAESGQAAAVELAKASKIVQQWIDNKPIKKTVYVEGRLVNLVV"
     gene            2900..>4602
                     /gene="glmS"
                     /locus_tag="PKK37_02805"
     CDS             2900..>4602
                     /gene="glmS"
                     /locus_tag="PKK37_02805"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNR81341.1"
                     /translation="MCGIIGYIGKGEAFPVLISGLKRMEYRGYDSFGFCIQGRDKNFS
                     SFKKVGKISGADGDLAKVSAPGNVGHAHTRWATHGGVTEVNAHPHLDCQGAISVVHNG
                     IIENYKELKKELEAEGHTFISQTDTEVLAHLIEKYFDGNLERAVKKSVARVRGTYGIV
                     VIAKNDPEKIVAARSGSPLLIGIGEDEFLVASDPAAVVANTKKVVYLNEGEIATLTPE
                     GYSIYAQREPQEIEWDLRDVEKGDYPHFMLKEIMEQPESLANSLRGRLLPEDGNAKLG
                     GLMPIEDRLRDIKKLHIVACGTARYAGLIAELMLEEYAGVPTDVDAASEFRYRKNILE
                     KDSAVLAISQSGETADTLAAIREAKSKGILTIGVTNAVESTQARETDAGIYNHAGPEI
                     GVASSKAFTSQLTVLALLTLYLGRQRDMALVMGQRIVNELARIPELAQKTIENCAGLK
                     EVAQTYKKYPSFFFMGRKYNFPIAMEGALKLKELAYVHAEGVASGELKHGMIALIEND
                     YPVIVICPSDSVYEKNISNIMELKARGARILAVATEGNEEIRDIADDVIYIPKTLEML
                     TP"
BASE COUNT         1343 a          858 c         1253 g         1148 t
ORIGIN      
        1 gggatgcggc gaagatatca ttaaagaatt tgaagaattg gcggagaaat attatcaaaa
       61 ataattttca gttttcaatt gccagttttc aatgaattat catttgttca atgatccaat
      121 ttacaaataa aatgcaagtc cgtatttggt ttggcccatt ggtgcattga aaattcaatg
      181 aaaattgaaa actggcaatt gaaaactgaa agataagcga agataaggta tttcaacatg
      241 gaaaatcaaa aaaacaaaaa agaatatcaa ccgcaaatga ttgagcccaa gtggcagaag
      301 atttgggagg aaagtaattt ataccgggcg caggattttt ccgataaacc caaggcatat
      361 cttttggtgg aatttcccta tccttccggc gagggattgc atgtggggca tgtgcgatca
      421 tattcggcaa tggattgcgt ggcgcgcaag cgccgaatgg aaggggagaa tgttttattt
      481 cctatcggct gggacgcgtt tggcttgccg gcggaaaact acgcgattaa aaccggtatt
      541 catccgagca ttacgacaca aaaaaatatt gccaattaca cgcgccagtt aaaaagtatg
      601 gggctttcct ttgattggtc gcgccaagtg gataccactg attctggcta ttataaatgg
      661 acgcagtgga tatttttaaa actttatgaa agagggatgg cttatcaggc cgagattccg
      721 gtaaattggt gcccgaaatg caaaaccgga ttggccaacg aagaagttat tgccggaaat
      781 tgcgaacgat gcggagaatc ggtaacgcgc aaaacattaa agcagtggat gctagccatc
      841 accaaatatg ccgatcggtt gatagatgat ttaagcagag ttgatttttg ggaaaaaata
      901 aaaattcagc agaaagaatg gattgggaaa agcgaaggag cgacaattga ctttgcgatt
      961 gccgattctg ccgaaagggt gagtattttt actacgcgca cggacacgat atttggcgtt
     1021 acggctttgg ttttggcgcc agagcacgaa ttggtggcta aattaaaagg ccaaattaaa
     1081 aattggaatg aggtttgcgc ttatattgga gcaacgaata aaaaatcgga atttgaaaga
     1141 ttgaatttgg aaaaaaagag aaccggtatt tgtttggagg gggtgcttgc gataaatccg
     1201 gcaaataatg aaaaaattcc ggtgtggctg ggagactatg tgattgcggc ctatggcggt
     1261 ggcgcggtga tgatggtgcc ggcgcacgac gcccgcgatt atgattttgc caggcgttat
     1321 caaataaaat caattttagt tgttcagcct cgttcaaaga atgatattac gacatataat
     1381 acccatccct cgtttgcgcc tgagattctg gaaggagaat ctgttgatga tgcgacagag
     1441 cgtaatagaa tgcaagaata caatttagtt ttaaatggaa aaagatgtta tacgggggga
     1501 ggtttgcttg taaattccgg agagtttaat ggtttaggtt ctgaaactgc cattgaaaaa
     1561 atttgtgagt ggttgcaaga gaagggagcg gccaaaaaaa ccgtgcatta caaattgcgc
     1621 gattggattt tttcgcgcca gcattattgg ggcgagccga ttccgattat tcattgcgaa
     1681 aaatgcggca ttgttccggt gccggaaaac gaattaccgg tcaaattgcc tcatattgag
     1741 aaataccagc ctacgggaac gggagagtcg ccgctggccg taattgatga atgggtaaat
     1801 gtcaaatgtc cgaaatgcgg cggcgcggca aaaagagaaa ccgataccat gcccaattgg
     1861 gcgggttcaa actggtatta tgtgcgttat tgcgacccgt ataatgaacg gcagattggc
     1921 gccgaagata agttggatta ttggctaccg gtggattggt ataatggagg aatggagcac
     1981 accactttgc atttgttata ttcgcggttt atttataaat tcttgtttga tatcggcgcg
     2041 gtgccgacgg ctgaacccta tcaaaaacgc acttcgcagg ggatggtgct ggcggcaaat
     2101 aatcagaaaa tgtccaagtc gcgcggcaat gtggtgaatc ccgatgatat cgtcaaagaa
     2161 tacggcgcgg atactttgcg gatttacgag ttgtttatgg ggccgtttga ccaggcgatt
     2221 gcgtggagtg cggatgcggt gcggggaagc cgcaagtttt tgggacgggt gtggaatttg
     2281 gtttttgaat gcgcgaacaa tgaaaatagc ggcgaggatg ttttgcgccg cgttcacaaa
     2341 ttaaatcaaa aaatcagcgg tgatattgag caattgaaat ttaatacggc gattgcggcg
     2401 tttatggaat tcttgaattt tgccgaagac aataaaagtg atgtcggcaa aaatgttttg
     2461 caaagattgg tgatgctgtt ggcgccgttc gcgccgcata tcggcgaaga gttatggcaa
     2521 aaactggaca ataatgaaag cgtttttaaa cagccatggc cgcaacctga caaaaatttg
     2581 gtcaaagacg aaacgattga actgttggtg caagtcaacg ggcggttgcg cgacaaaatc
     2641 gcggttccgg ccgaatccgg ccaggccgcg gccgttgaac ttgccaaagc gtcaaagata
     2701 gtgcaacaat ggatagacaa taaaccgatc aaaaagacgg tttatgttga aggcaggctg
     2761 gtcaatcttg ttgtctagaa ttaaaaatgt aaaaatcaaa aatcaaaatg acaatgcaaa
     2821 atgtaaaatt ttggatttga taatttttaa tttttcattt gaatttttaa ttttgatatt
     2881 taaatttttc atttggaaca tgtgcggaat aatcggctac attggcaagg gggaggcgtt
     2941 tccggtgctc atttccgggc ttaagcggat ggaataccgc ggctatgaca gctttggttt
     3001 ttgcatccaa ggccgggata aaaacttttc ctcgttcaag aaagtgggca agatttcggg
     3061 cgcggacggg gatttggcca aggtaagcgc gccgggcaat gtgggccacg cgcacacccg
     3121 ctgggccacg cacggggggg tgaccgaggt taacgcccat ccgcatttgg actgccaagg
     3181 cgcgatctcg gtggtgcaca acggcatcat tgaaaattac aaagagctta aaaaagaatt
     3241 ggaggccgaa ggccacacct tcatttccca aaccgacacc gaagtgctgg cacacttgat
     3301 tgaaaaatat tttgacggca atttggagcg ggcggtgaaa aaatcggtgg cgcgcgtgcg
     3361 gggaacctat ggcatcgtgg tgatcgccaa aaacgatccg gaaaaaatcg tcgcggcccg
     3421 gtcgggcagt ccgttgttga tcggcatcgg cgaggatgaa tttttggtgg cgtcggatcc
     3481 ggcggcggtg gtggccaaca ccaaaaaggt ggtctatctc aacgagggcg agattgccac
     3541 cctcaccccc gagggttatt caatctacgc ccagcgcgag ccgcaagaaa ttgagtggga
     3601 tttaagagat gtggaaaaag gcgactatcc gcattttatg ctcaaagaga ttatggaaca
     3661 gcccgaaagt ttggccaact cattgcgcgg ccggctgttg cccgaagacg gcaatgccaa
     3721 attgggcggc ctgatgccca ttgaagaccg tctgcgcgat atcaaaaaac ttcatattgt
     3781 ggcgtgcggc acggcgcgat atgccggatt gattgcggaa ttgatgctgg aagaatatgc
     3841 cggcgttccc acggatgtgg acgcggcttc ggagtttcgt tatcgtaaaa atatcttgga
     3901 aaaagattcg gcggtgttgg cgatttccca atcaggcgag accgcggata ctttggccgc
     3961 gattcgcgaa gctaaatcaa aaggaatttt gactattggc gttaccaatg cggtggaatc
     4021 aacccaggcg cgcgaaaccg atgccggaat ttataatcat gccggaccgg aaataggagt
     4081 ggcgtcgtca aaagcattta cttcgcaact aaccgttttg gcattattaa cattatactt
     4141 ggggcgtcaa agagatatgg cgctggtaat ggggcaaagg attgtcaacg aattggcgcg
     4201 gattcctgaa ttggctcaaa aaacaattga aaattgcgcc ggattaaaag aggttgcgca
     4261 aacttacaaa aagtatccca gtttcttttt tatgggccgc aaatataatt ttccgattgc
     4321 catggaaggg gcgcttaaat taaaagaatt ggcttatgtt cacgccgaag gagtggcgtc
     4381 gggagagttg aaacatggaa tgatcgcatt gatagaaaac gattatccgg tgatagtaat
     4441 ttgtcccagc gattcggttt atgaaaaaaa catttccaat attatggaat tgaaagctcg
     4501 cggggcgcgg atattggcgg tggccaccga gggcaatgaa gaaatccgcg acattgccga
     4561 tgacgtgatt tatatcccga aaactttgga aatgctcacg cc
//
LOCUS       DAOQWE010000062         4548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_19377_length_4548_cov_6.385266, whole genome shotgun sequence.
ACCESSION   DAOQWE010000062 DAOQWE010000000
VERSION     DAOQWE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19377_length_4548_cov_6.385266"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="PKK37_02810"
     CDS             <1..116
                     /locus_tag="PKK37_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006790628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81342.1"
                     /translation="DIVDFGVPLLSMHAPFEIASKADIYSAYLAYKAFFEN"
     gene            126..533
                     /locus_tag="PKK37_02815"
     CDS             126..533
                     /locus_tag="PKK37_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81343.1"
                     /translation="MNTKIIMGWVLIVAGLAVIGAAINASYRYFTAKAEFPAVFEISA
                     KNPAGDLPSQAGIENLSVEEAQNYLQNQIQYSVNRAVVDMLPDDAVAKMLNILSWSIF
                     GTFLIFAGGKISWLGVQLLSVRNESAQKTNQPS"
     gene            complement(553..1188)
                     /gene="pcm"
                     /locus_tag="PKK37_02820"
     CDS             complement(553..1188)
                     /gene="pcm"
                     /locus_tag="PKK37_02820"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNR81344.1"
                     /translation="MALIDDLINAGYLKTPRIIAAFRAIKRKDFLPPDLPHRCIAAEM
                     DEAVAIGFAQTISQPATVAFMLELLQPRPGDKIMDIGYGSGWTGALLAHIVSQNGARG
                     KVVAIERIKPLAEFGKENIAKYGFVKNRTVQCVIGDGSKGYLPQAPYDRILASAAAPS
                     VPPAWEAQLKTGGTIVAPVQNALAKIEKSKNNTFSETRYEGFAFVPLVQGS"
     assembly_gap    1339..1438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1439..2485)
                     /locus_tag="PKK37_02825"
     CDS             complement(<1439..2485)
                     /locus_tag="PKK37_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNR81345.1"
                     /translation="MKKNQKGSAILAILIVLILAIAAAIGAGWYAYRFYSAPPENIDM
                     PEINFSASPELLDENPPNENEGDGETIPEAEGSVASPETFVEYETGQDSSPIVNAANQ
                     FALELYGEYKNTNDNLFFSPHSISSALQMTYEGARGKTAAEMRSVFHFPEDNAARIGS
                     FAKLYEQINPQNANYRLSTANALWAQTDYPFDQNYLKTAEIYYHGKAANLDFAGDTEN
                     SRKTINEWVSGKTAQKIPELFAAGTLNPLTRLVLTNAVYFKGKWATPFEEMLTQEKDF
                     TASTGAKIKCQMMNIKDDFGYAQTADYQALELPYEDSDLSMIVVLPENGKIEATEKEL
                     AAAGLGNVKKSLKNE"
     gene            complement(2552..4282)
                     /locus_tag="PKK37_02830"
     CDS             complement(2552..4282)
                     /locus_tag="PKK37_02830"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNR81346.1"
                     /translation="MIKTIKILILVAVLAQTAIAPSALARDTNQITDWYIKDFQSTIT
                     VNTDSSLLVEEKIIADCGDLPDKHGIFRVVPLETKTEKGTVKTPVELISIADFDDRPI
                     KYSETKDNFNHTVAWKIGDADKNVTGENYYKIIYSVKNAVRAGNADFDELYWNLLGNF
                     WDLEIENFRADIIFPEGISQNNTDIYLYAGQLGAEDNRLTKREWIDSGALRITSTSAL
                     SPRQGVTVSATFPKGIIAPYQPGFFELYGDYLWFLIPILTFIICFAVWNKYGKDPRVD
                     KTVIPEFEIPENLTPMQMGLLFKNGSFGDSLISATIIDLAVKKYIIIEEIPKQGIFGQ
                     QNFRLKRIEGSGYPSPNETEELLLKKLFGPSQEVLLSKLKNEFHKELPEIKKTAINNL
                     KQKEFIHPSGLTFKRIFLALAVILIFAAFAGAGTENLFAIIAFGISAAVVLIFGLLMP
                     KRTPKGAELNWRIKGFKLYMETAEKYRTRFHEKENIFEKFLPYAIVFGIAKLWVKKME
                     QIYGKEYFAAYHPAWYAGNFASGFNADSFAAQINSISSGIAANIGTTSGAHGAGGAGG
                     GGGGGGGGGW"
BASE COUNT         1068 a         1061 c         1048 g         1271 t
ORIGIN      
        1 tggatattgt tgattttggc gttcccttgc tgtcaatgca cgcgccgttt gagattgcgt
       61 caaaggccga tatatattcg gcgtatttgg cgtataaagc gttttttgag aactaaatct
      121 aaagtatgaa cacaaaaatt atcatgggtt gggttttgat tgttgcggga ttggcggtga
      181 ttggtgccgc gatcaacgca tcataccgct atttcacggc caaagccgaa tttccggcgg
      241 tgtttgaaat ctcggcaaaa aatccggcgg gcgatttgcc gtcgcaagcc ggaattgaaa
      301 atctttcggt ggaagaagcg cagaattatt tgcaaaacca aatacaatat tcggttaacc
      361 gtgccgtggt tgacatgctt cccgacgacg cggtcgccaa aatgctcaac attctttcgt
      421 ggtcaatctt tggcacattt ttgattttcg cgggcggaaa aatttcctgg ctgggcgtgc
      481 agttattgtc cgtgagaaat gaaagcgcgc aaaaaacgaa tcaaccaagc tagcggtggg
      541 gcgggcgaaa tatcagcttc cctgcaccag cggcacgaat gcgaatcctt catagcgggt
      601 ttcgctgaaa gtgttgtttt tgcttttttc aatctttgcc agcgcgtttt gcaccggcgc
      661 cacgatcgtg ccgccggttt tcagttgcgc ttcccacgcc ggcggaacgc tcggtgcggc
      721 cgcggatgcc aaaatccggt cgtaaggcgc ttgcggcaaa tacccttttg atccgtcgcc
      781 gatcacgcat tggacggtgc ggtttttaac gaaaccgtat ttggcgatgt tttctttgcc
      841 gaattcggcc agcggcttta tcctttcaat cgccaccact tttccccgcg cgccgttttg
      901 cgaaacgatg tgcgccaaaa gcgcgccggt ccatcccgat ccgtagccga tgtccataat
      961 tttatcgccc ggacggggtt gcagaagctc cagcataaac gccacggttg ccggctggga
     1021 aatggtttgc gcgaatccga tagcaacggc ctcatccatt tcggccgcga tgcaccggtg
     1081 cggcaaatcc gggggcaaaa agtcttttct cttgatcgcg cgaaatgccg cgataatccg
     1141 cggagttttc aaatagcctg cattgatcaa atcgtcaatt aatgccataa attttgccaa
     1201 aaagcgcccg aaaggcgcct tttgcggtta aacgattatg tccggatgtt tttatgggca
     1261 taatgccata tattattttg tcgggtcgtt gacttttccc ataaacaaga tcgcgccggt
     1321 ttggttgtgg acgatggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     1441 cgttttttaa agatttttta acattaccca gaccggcggc cgccaattct ttttccgtgg
     1501 cctcaatttt accgttttcg ggcaaaacga cgatcattga caaatcgctg tcttcatagg
     1561 gcaattccag cgcttgatag tcggcggttt gggcatatcc gaaatcatct ttgatgttca
     1621 tcatctggca tttgattttt gcgccggttg atgcggtgaa atctttttcc tgagtcagca
     1681 tttcttcaaa cggggtcgcc cacttgcctt tgaaataaac cgcgttggtc aaaaccaatc
     1741 ttgtcaacgg attgagcgtt cccgccgcga acaattccgg aattttttgc gcggttttgc
     1801 cgctcaccca ttcattgatg gttttgcggg aattttccgt gtcgccggca aaatccaaat
     1861 tggcggcttt gccgtgataa tagatttccg cggtttttaa ataattttgg tcaaacggat
     1921 aatccgtttg cgcccaaagc gcgttggcgg tggaaagccg gtagttcgcg ttttgcgggt
     1981 ttatctgttc ataaagcttg gcaaacgatc cgatgcgcgc ggcgttgtct tcgggaaaat
     2041 gaaacaccga tcgcatttct gcggcggttt ttccgcgcgc gccctcataa gtcatttgca
     2101 gagccgaaga aatgctgtgg ggcgagaaaa agagattatc gtttgtgttt ttatattcgc
     2161 cgtatagttc aagcgcgaat tgattggcgg cgtttacgat cggggatgaa tcctgtccgg
     2221 tctcgtattc aacaaatgtc tccggcgaag caacggaccc ttccgcttcc gggattgttt
     2281 ccccgtcccc ttcattttcg ttcggcggat tttcgtccaa aagttcggga ctggcggaaa
     2341 aattaatttc cggcatatca atattttccg gcggcgcgga ataaaaacga taggcatacc
     2401 agccggcgcc gatcgccgcg gcgatggcca gtatcaacac gatcaatatg gccaatattg
     2461 cgcttccttt ttgatttttc ttcataattt tagattgtct ggattaaaat ggtttgctcc
     2521 tgtcttaaga ataacataat cggcaaaaaa atcaccaacc gcctccccct ccgccgcctc
     2581 cccctccgcc cgcgccgccg gcgccgtggg cgcccgaagt tgtgccgatg ttggcggcga
     2641 tgccggatga gattgaattt atttgtgccg caaaactgtc ggcgttgaat ccggaagcga
     2701 aattacccgc gtaccaggcc ggatgatagg cggcaaaata ttccttgccg tagatttgtt
     2761 ccattttttt gacccaaagt ttggcgatgc cgaaaacgat cgcatagggc aagaattttt
     2821 caaaaatatt ttctttttca tggaatcgcg tccggtattt ttcggcggtt tccatataga
     2881 gcttgaaacc tttgatgcgc caattcagct ccgcgccttt tggcgtgcgc ttgggcatca
     2941 ataagccgaa aatcaacacc acggcggccg aaattccaaa agcgatgatg gcaaataaat
     3001 tttccgtgcc ggcgccggca aaggcggcga agattaaaat aaccgccaaa gccagaaata
     3061 tccttttaaa cgttaagccg cttggatgaa taaattcctt ttgtttcaga ttgtttattg
     3121 cggttttttt gatctcgggc aattctttgt gaaattcgtt ttttaatttt gacagcaaaa
     3181 cctcctgact ggggccgaat aattttttaa gcaacaattc ttcggtttca ttcggcgaag
     3241 gatagccact gccctcaatt cgtttcaggc gaaaattctg ctggccgaat atgccttgtt
     3301 tgggaatttc ttcaatgatg atatattttt taactgccag atcaatgatc gttgccgata
     3361 tcaatgaatc gccaaagctt ccgtttttaa acaacaaacc catttgcatc ggcgtcaggt
     3421 tctccggaat ttcaaattcc ggaatcacgg ttttgtcaac gcgcggatct ttgccgtatt
     3481 tgttccaaac cgcaaaacaa attatgaaag tcaaaatcgg gatcagaaac cacaaataat
     3541 cgccgtaaag ttcaaaaaat ccaggctggt atggcgcgat aatacccttg gggaaagttg
     3601 ccgataccgt gactccctgc cgcgggctta atgccgacgt tgacgttatc cgcaaagcgc
     3661 ccgaatcaat ccattcgcgc ttggttaaac ggttgtcttc ggcgcccaat tgaccggcat
     3721 acagataaat gtcggtgttg ttttgactga tgccttcggg aaaaataatg tcggcgcgaa
     3781 aattttcaat ttccagatcc caaaaatttc ccagcaaatt ccagtagagt tcatcaaaat
     3841 ctgcgttgcc ggcgcgaacc gcgtttttaa ccgaataaat tattttgtaa tagttttcgc
     3901 ccgtaacgtt tttatcggca tcgccgattt tccaagcgac ggtatggttg aaattgtctt
     3961 tggtctcgga atattttatc ggccgatcgt caaaatccgc gattgaaatc aattccacgg
     4021 gagttttaac ggttcccttt tcggtttttg tttcaagcgg caccacgcgg aaaattccgt
     4081 gcttgtcggg taaatcgccg caatcggcaa tgatcttttc ttccaccaat aaagatgaat
     4141 cggtattgac cgttatcgtt gactgaaaat ccttaatgta ccaatcagta atctgatttg
     4201 tatcccttgc cagcgcggat ggcgcgattg cggtttgggc caaaaccgca accaaaatta
     4261 aaattttaat tgtcttaatc ataaaatacc ttgaattaaa aagcttatta aatagacctg
     4321 ctgatcaaag atttcctact gcaatttcca tatttcggct acgccgaatt catcgttcgt
     4381 cggcagactt tagcttcaag tatgcctcct cgctcttcat tcggctcgct caaaatctga
     4441 aaatttcgcc acggaaatct ttgatcagca ggtctaatga tcattcaatg aagccggaag
     4501 attgcaagcg ccagagtttg cggtagacgc cgtccctttt gcggatca
//
LOCUS       DAOQWE010000063         4517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_19568_length_4517_cov_7.449126, whole genome shotgun sequence.
ACCESSION   DAOQWE010000063 DAOQWE010000000
VERSION     DAOQWE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19568_length_4517_cov_7.449126"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..445
                     /locus_tag="PKK37_02835"
     CDS             17..445
                     /locus_tag="PKK37_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81347.1"
                     /translation="MFDSNKTTKLLFACLDWRLHPAIENYFATKDVACDMCITAGSLK
                     GLIDPATQDFFLKQIETSRNLHHCREAILTMHMDCGAYGGSAAFSGPEEEIACYKEEL
                     RRAKEIIGKKFPELAVKTWLIGLDGKNGEWEISPKEINLD"
     gene            478..1608
                     /locus_tag="PKK37_02840"
     CDS             478..1608
                     /locus_tag="PKK37_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HNR81348.1"
                     /translation="MAKFNNLKTFLYSWGVLTNTIIGIGIFALPYAALKVGLPLALLY
                     LAGLGAVVGLIHSMFGELALKTPDYKRLVGFAKIHLGRWAQAVAVAISIIGFFGSLLA
                     YIIIGGGFLYQFCAPFLGGSETLYGVVYFALGALFVYLGIAFISRFEFWGLVLFFMIL
                     LLLAVQGMPLISWENFTARTGGAADIFFPYGPILFAFWATSSIPEIEEMLGRNGGKKM
                     LGKVISASMATVFFTYLLFIFFIVGIGGAQTAESALIGLQDALGANVVAILFFLGFIT
                     TFSSFIITGLSLKKILAYDLKINKNLAWLITSAVPITLFLSGLRDFMMIFSLIGGVFL
                     AIDGILIILMYQKIRPDRKMICWPLLLILAGGIIYELVYYFV"
     gene            1627..2589
                     /locus_tag="PKK37_02845"
     CDS             1627..2589
                     /locus_tag="PKK37_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNR81349.1"
                     /translation="MALSYVIIFLLCCAALYFAGNWVIAGTARLAKFFGLKEFVVAFF
                     IMALAATLPNLFLAIMAVANGIPELSLGDILGGNVVDMTLAVALAAFFSKKGIDAKGQ
                     TIQASLMFTFAAAVLPLALLLDNHLSRIDGILLLALFFVYVYWLLSKKERFKTIYDGH
                     QVSIGKQIKHFWQDIARVAGGVAGMIIVAQIIILAANRFSSELAVALPLVGILIVGLG
                     NSFPEIYFGIAAAKSGNTKMILGDLMGAVIMSGTMVLGLTALASPIKINDLEMFGAAR
                     YFLFGAAVFFYICARSGQKISKKEGLLLLGIYIAFLITEVFARH"
     gene            complement(2560..4514)
                     /gene="uvrB"
                     /locus_tag="PKK37_02850"
                     /pseudo
     CDS             complement(2560..4514)
                     /gene="uvrB"
                     /locus_tag="PKK37_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
BASE COUNT          955 a         1046 c         1177 g         1339 t
ORIGIN      
        1 ttagatttta atttttatgt ttgattccaa caaaaccaca aagctgcttt ttgcctgtct
       61 ggattggaga ttgcatccgg cgattgaaaa ttatttcgcg accaaagatg tcgcgtgcga
      121 tatgtgcatt accgccggat cgttaaaggg attgattgat cccgcgacgc aggatttttt
      181 tctcaaacag attgaaactt cccgaaattt gcaccactgc cgggaagcaa tcctcacgat
      241 gcatatggat tgcggcgcct atggcggatc ggcggcgttt tccggccccg aagaagaaat
      301 cgcttgctat aaagaagaat tgcgccgggc caaagaaatc atcggcaaga aatttcccga
      361 attggcggta aaaacttggc tgatcgggct tgacggaaaa aatggcgaat gggaaatatc
      421 gcccaaagaa ataaatcttg attgaaaggg tccaaccctt ttgaagttgt gcgcgatatg
      481 gctaaattta acaatcttaa aacttttctc tattcatggg gcgtgctcac caacacgatc
      541 atcggcatcg ggatttttgc cctaccctac gcggcgctga aagtcggcct gccgctggcc
      601 ctgctctatt tggccggatt gggcgcggtg gtggggctga ttcattcaat gtttggcgag
      661 ttggcgctaa aaacccccga ctacaaacga ttggtggggt tcgcaaaaat tcatttgggc
      721 cggtgggcgc aagcggtggc ggtggcaatc agtatcatcg gatttttcgg ttcgcttttg
      781 gcatatatta tcatcggcgg gggatttctt tatcaatttt gcgccccgtt tttgggcgga
      841 tcggaaaccc tttacggcgt tgtttatttc gcgctgggag cattgtttgt ttatttgggg
      901 attgctttta tttcacggtt tgaattttgg ggactggtgc tgttttttat gattctgctt
      961 ttgctggcgg tgcaaggaat gccgctgatc tcctgggaaa atttcactgc gcgcacgggc
     1021 ggcgcggcgg atattttctt cccctacgga ccgatattgt ttgccttttg ggccacttct
     1081 tccattccgg aaattgaaga aatgctgggg cgcaacggcg gcaaaaagat gctgggcaaa
     1141 gtcatttcgg cgtcaatggc caccgtgttt ttcacttatc ttttatttat ttttttcatc
     1201 gtgggtattg gcggcgcgca aaccgccgaa tccgcgctga ttggattgca agatgcgctg
     1261 ggcgcgaatg tcgttgccat attgtttttt ttgggattta tcaccacttt cagttcgttt
     1321 atcatcaccg gcctatcgtt gaaaaaaatt ctggcctatg atttgaagat caacaagaat
     1381 ttggcctggc tgattacttc ggcggtgccg atcacgcttt ttttaagcgg tttgcgcgat
     1441 tttatgatga tcttctcgct gattggcggc gtttttctgg caattgacgg cattttgatt
     1501 attttgatgt atcaaaaaat ccgcccggac aggaaaatga tttgctggcc gttgctttta
     1561 attctggccg gcggtataat ctatgaattg gtctattatt ttgtctaata taaatttgtt
     1621 ttaacgatgg cgctctcata cgtcattatt tttcttctgt gttgcgccgc gctttatttc
     1681 gcgggcaact gggtaatcgc cggcacggca cgtttggcca agtttttcgg gctgaaagaa
     1741 ttcgtcgtgg cgttttttat tatggcgctg gcggccactc tgccgaatct gtttttggcg
     1801 ataatggccg tcgccaacgg cattcccgaa ctttcgctgg gcgacatttt gggcggcaac
     1861 gtggtggata tgacattggc cgtggcgctg gcggcgttct tttccaaaaa ggggattgat
     1921 gccaaggggc aaacgattca ggccagcttg atgttcactt tcgccgcggc ggttctgccg
     1981 ctggcgcttt tgctggacaa ccatttatca aggattgacg gcatattgct tttggcgctg
     2041 tttttcgttt atgtctattg gctgttgtcc aaaaaagaaa gattcaagac gatttatgac
     2101 ggccatcagg tttccatcgg caagcaaatc aagcattttt ggcaggatat cgcccgggtg
     2161 gcgggggggg ttgcgggaat gatcattgtg gcgcaaatca ttattcttgc ggccaatcga
     2221 ttttcttcgg aattggcggt tgccctgccg ctggtcggca ttttgatcgt cggtttggga
     2281 aattcgtttc cggaaatata ctttggcatt gccgcggcca aaagcggcaa cacgaaaatg
     2341 atcttgggcg atttaatggg cgcggtgatt atgtcgggaa cgatggtgtt gggattgacg
     2401 gcgctggcct cgccgataaa gatcaacgat ttggaaatgt ttggcgcggc gcggtatttt
     2461 cttttcggcg ccgcggtttt cttctacatt tgcgcccgga gcggccagaa aatcagcaaa
     2521 aaggaagggt tactgctctt ggggatttat atcgcttttt taatcaccga ggttttcgcg
     2581 cggcattgat ttttctttga cacgggcgat ttcggcttgg atttgcgcgg ccttttcaaa
     2641 ttgcagattg cggcgtgcca gatccagttt ggcgcccagc tctttttgat attctctgat
     2701 aaattcgtcg tcgggcaatc tttcttcttt tttgtcgcgc gccgacaagg tttctttaat
     2761 ctctttataa atcacttccg gcgaaatgcc atgtttttta ttatatgctt cttgaacctt
     2821 gcgccgccgg ccggtttctt caatcgcgcg cgccattgat ccggtggtgt tgtcggcata
     2881 cataatcacc aatccttgcg gatggcggct ggcgcgtccc attatctgca ccagcgaagt
     2941 ttcgtcgcgc aaaaaacctt ccttgtcggc gtcaaaaatt ataatcaaag aaacttccgg
     3001 cagatcaagt ccctcgcgca aaagattgat ccccaccaaa acatcaaatt ttcctttgcg
     3061 caaatccgcc agaattttgg gccgcgccaa agttttaatt tccgaatgca gatactcggc
     3121 gcttattttt ttacttttaa ggtggtcagc catcgcttca gccaattttt tggtgatagt
     3181 caggcataat gtgcgctggc cgattttttt gcgcttttca atttctttga ttgcgtcggc
     3241 gatttgattt tccgtggggc ggatgctgat atcgggatcc agcagatagg tggggcgcac
     3301 gatttgttcg gcaatttgcg cggaaacttt cttttcataa tcgccgggcg tggccgacac
     3361 gaacaaaact tgattgattt tttttaggaa ctcgttatat ttcagcggcc ggttgtcaat
     3421 cgccgacggc aggcggaaac catagttgat cagcggctgt ttgcgggcga aatcgccggc
     3481 ctgcatgccg tgaatttgcg gcacggcaat gtgcgattcg tcaatcatca ccaagtaatc
     3541 ccgggggaaa taatccagca gggtataggg cgccgaatcg ggttttctga aatccagatg
     3601 ccgcgaatag ttttcaatgc cgcggcaact gccggtttcg cgaatcagcg aaatgtcata
     3661 ttcggtgcgc ggcggatccg ttcggcttcc atcaattttt cccgtttttt gaaaaattcc
     3721 gcttgttctt ccatttcggc gcggatattt ttgatggaaa tttcgatctt ctcctggtcg
     3781 gccatccaaa attttgccgg ccaaaaatcg gcttcgccgg cgagttcaaa tttgccgaac
     3841 ggcgcggggc tttgggcgat gcttttaatc gcgccgccgg caatttccac gcggcaaatg
     3901 gtttgcgcgc cggccagcca cacatcaaca aaattcatcc gcttgcggaa gcgcccgggc
     3961 gccagttcgt aatcatttct ttcatattgc aaatccagca ggcggccgaa aaattgttca
     4021 aaggttgccg gatcgccggg cttggcaatg aaccgctcgc gcaagtaggt ttgcggcgat
     4081 cccaaattgt aaatgcagga aaccgacgcc accacgatcg cgtcgcgccg ggaaagcacc
     4141 atctgcaccg cttcgtggcg caggcgatca atttcctcgt tgattttaga gtctttttca
     4201 atgtaggtgt cggtggcggg aatataggct tcgggctggt aataatcata atacgacaca
     4261 aaatagccca ccgcgttgtt gggaaaaaat cctttgaact cttcgtataa ttgcgccgct
     4321 aatgtcttgt tgggtgaaat gaccaaagtc ggccggccgg ttttttcaat taccttggcc
     4381 atggtataag tcttccccga cccggtcacg cccaaaagcg tctggaattg atttccggcg
     4441 ccaatccctg cggccagctt gtcaatcgcc tgcctctgat ctccccgcgg ctcaaacttt
     4501 gattcaattt tcagttt
//
LOCUS       DAOQWE010000064         4506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_19624_length_4506_cov_5.521231, whole genome shotgun sequence.
ACCESSION   DAOQWE010000064 DAOQWE010000000
VERSION     DAOQWE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19624_length_4506_cov_5.521231"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1605)
                     /locus_tag="PKK37_02855"
     CDS             complement(235..1605)
                     /locus_tag="PKK37_02855"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HNR81350.1"
                     /translation="MSASYFSDNYISGIYPLGTCRSCSAQGKNCGIASDQCGGYIYCG
                     ACASGQVCSEAGQCVAQGVCTAGEVSGCKVCKTDGLGWADDSSKCADGKKCSLGQCVS
                     IPSDSDCTAKTCSSAGYECGAWNDACGGIFDCGACAAGKSCSAAGKCVDGAGTPQTDI
                     KINGDDGPVSAKPNTLITLSWISNNTTACTASGNWSGSKALSGYESIVMSSENMVFSI
                     SCTGDSGTVSDSITVNAAAFGISLAESIEVSSGATADLNAKITGTAPSSPVYQWSCTG
                     GSLSDSSILAPVYTAPAVTSNEQYVCTLTVRDGNSAGISKKININVKTVPQNVAELEK
                     QIAEIKALIAALQKQLAALAGLPATESSTGNAKYSCAQLTKYLIYNTQNDPEVKCLQE
                     FLIAQGFSGVAAIGNYDAATRDAVKTFQEKYAAEILAPYGLTYGSGNVGNDTRRKINA
                     LIAAGQ"
     gene            complement(1617..2024)
                     /locus_tag="PKK37_02860"
     CDS             complement(1617..2024)
                     /locus_tag="PKK37_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81351.1"
                     /translation="MERGERNYMVMDAIFMDAIVVSAMPRAMIFIIAVISVPLTQELR
                     KVALTAKSALQVRFAIPQSAVLRVMAVMAADHLPNSVTLSGRSGKIFGRNTHGTVRIG
                     RCLMPGAGDVMRMALAQRELLANLLTRSQNGTA"
     gene            complement(2156..2473)
                     /locus_tag="PKK37_02865"
     CDS             complement(2156..2473)
                     /locus_tag="PKK37_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81352.1"
                     /translation="MITKKALEKARILAFWEKHGLKATIDHSGKAKRTLQLACIFVFV
                     LKLKKPQVCEIIKLKKYEISFGCRALRVIVSVFFRRYWRCFRRGKFGAMHPRQRLRFR
                     TLQ"
     gene            complement(2667..>4506)
                     /gene="ligA"
                     /locus_tag="PKK37_02870"
     CDS             complement(2667..>4506)
                     /gene="ligA"
                     /locus_tag="PKK37_02870"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNR81353.1"
                     /translation="SPMLSLNDAFSKQDMLDWQERIGRLLSDAERARIDFYCEPKLDG
                     LAIELIYEDGILAAGATRGDGRVGENVTNNLKTIEAIPLRLRAAEEIAADLRAAGLKD
                     MAAAVTRGGWRHLAVRGEVVVSRRDFDAINDNQRKKGLPEFANPRNLAAGSIRQLDPK
                     IAASRRLDSNCYKLVTDLGQKTHEEEHRFLEIAGFKTNNKFSRFCAKMQEVFVFYRHW
                     RDHRGRLPYEIDGIVVAVNRNDLFEKLGIAGKSPRAAIAYKFPLKQATTVVEDIVVQV
                     GRTGALTPVAILRPVKVGGVMISRATLHNEDEIRRLGVKIGDTVIVGRAGDVIPGIIA
                     VLPELRTGDETAFKMPEICPMCGGKTEKKPGEAVLRCINRDCFAVQKRSLRHFVSRGA
                     FNIDGLGEKIIEQLIDQGLVRAPADIFGLKEGDLAPLARFGEKSARNLVESVDAKKTI
                     GLPQFIYALGIRNAGEQTARALAEKFGNIESIRSASFEDLQTVADIGPVVAESVCRWF
                     ADRKNTAVIEGLLSAGVRILPHQKTAGALAGKTFVITGTLEKMSRQAAKDAVRAAAGN
                     VSEAVSRNTDYLVAGANPGSKLEKARQSGVAVIGEEDFLRLITDNQ"
BASE COUNT         1034 a         1290 c         1113 g         1069 t
ORIGIN      
        1 tactgcaatg tcctgaatcg caatcgctgt cttgggtgca tggcgccaaa tttgcggccg
       61 ccgcacccga aaattcgtgc gtgtgatgat acaccgccaa gaacaccgcc gccaacgtgg
      121 cggcaaaccc cgcaataacg atttttttca ttattttggc ttatttgcgg cgcgcaatgt
      181 aagcgccggc ggatggcaag aatcgttacc atctcgccgg ccaacgccac ataattattg
      241 cccggcggca atcagcgcgt tgatcttccg gcgcgtgtca ttgcccacgt tacccgaacc
      301 ataggttaga ccatagggcg ccaaaatctc ggcggcatat ttttcttgga aagttttaac
      361 tgcatcgcgt gtcgccgcat cataattccc gatggccgcc acgccggaaa atccctgtgc
      421 aatcaaaaat tcctgcaaac atttgacctc cggatcgttt tgcgtattat agatgagata
      481 tttcgtcaat tgggcgcaag aatattttgc atttcccgtt gatgattccg tggccggcaa
      541 gccggccaac gccgccaact gtttctgcaa ggccgcaatc aacgctttga tctcggcaat
      601 ctgtttttcc aattcggcaa cattttgcgg caccgtttta acgttgatat tgattttttt
      661 ggaaatgccg gcgctattgc cgtcgcgaac cgtgagagtg caaacatact gttcgtttga
      721 agttacggca ggcgcggtat aaaccggagc caaaattgaa ctgtccgaca aactgccgcc
      781 ggtgcaactc cattgataaa ctggcgaaga aggcgccgtg cccgtaattt tcgcgttcaa
      841 atcggcggtc gcgccggaag aaacttcaat tgactccgcc aaactgattc cgaacgccgc
      901 cgcattcacc gtgatgctgt cgcttacggt tcccgaatcg cccgtgcatg agatggaaaa
      961 aaccatattt tcggacgaca taacgatgct ttcatatccg gaaagagctt tgctgccgga
     1021 ccaattcccg gatgccgtgc aggcagtggt attgttggaa atccacgata aagtgattaa
     1081 ggtgttcggc ttggccgata ccgggccgtc atcgccgttg attttaatgt ccgtttgcgg
     1141 cgtgccggcg ccgtcaacgc atttgcccgc cgcggaacac gatttgcccg ccgcgcacgc
     1201 gccgcaatca aaaattccgc cgcaggcgtc attccacgcg ccgcattcat atccggccga
     1261 agaacaggtt ttggcggtgc aatcggaatc cgaggggatg gaaacacatt gtcccagcga
     1321 acactttttc ccgtcagcgc attttgaact gtcatcggcc catcccaatc cgtcggtttt
     1381 gcaaactttg catccggaaa cttctccggc ggtgcacact ccttgcgcca cgcactggcc
     1441 cgcttcgcta caaacttggc ccgaggcgca agcgccgcaa taaatatacc cgccgcactg
     1501 atcagacgca atgccacagt ttttgccctg cgccgaacaa ctgcgacaag ttcccaatgg
     1561 atatattccg gaaatataat tatcgctaaa gtaagacgcg gacatcggga gagagcttac
     1621 gcagtaccat tctgcgatct cgtcaataaa ttggcaagta gttcccgctg ggcaagcgcc
     1681 atgcgcatga catccccagc tcccggcatc aagcatctgc caattcttac cgttccatgt
     1741 gtgttgcgcc caaatatttt gccggatcgc cccgacagcg tgacactgtt tggcaaatga
     1801 tccgccgcca taaccgccat aacccggagg acagcagatt gtggtattgc aaaccgtacc
     1861 tgcagggcag acttcgccgt tagcgcaact ttgcgcaact cctgggtcaa cggcactgaa
     1921 atcacggcaa taataaaaat catagccctc ggcatagcac ttaccactat cgcatccata
     1981 aagatcgcat ccataaccat atagttgcgt tctccacgtt ccattgcggc aaatcagttt
     2041 agccctgtga tttcctatcg tttgaccatc ggccacgcat gtgcgagcaa gcgatccctc
     2101 gccccagccg caagtgccag agggacagca aatcccctga gcggcgcccg catcattact
     2161 gcaatgtcct gaatcgcaat cgctgtcttg ggtgcatggc gccaaatttg ccgcggcgga
     2221 aacagcgcca ataccggcga aaaaaaacac tgacgataac gcgcaaagcg cggcaaccaa
     2281 acgaaatttc atattttttt aattttatta tttcgcagac ctgcggcttt tttaatttta
     2341 acacaaaaac aaaaatacaa gcaagttgca aggtgcgctt ggctttgccc gaatggtcaa
     2401 tggtagcttt gaggccatgc ttttcccaga atgccagaat acgagctttc tccaatgctt
     2461 ttttagtgat catataccga taccttaatg catcagtata taccgttaaa aagcctttga
     2521 ttccagtata tttgcagtga atatgattgt aaaattccat gaagctacgg cttcaaggag
     2581 tgcaatatgt atgtgagctt agacagcaac gcccggcgca acagtcattt aggcagttta
     2641 aatatttatc gtttatcgtt tattggttat tggttatcgg ttatcaatct caaaaaatct
     2701 tcttcgccga tgacagccac ccccgactgc cgcgcttttt ccagcttgga gccgggattg
     2761 gcgccggcga ccaaataatc ggtgttccgg gaaaccgctt cggaaacatt acccgcggcg
     2821 gcgcggaccg catctttggc ggcctggcgg ctcatttttt ccagcgtgcc ggtgatcaca
     2881 aaagtttttc cggccagcgc cccggcggtt ttttgatggg gcaaaatcct gacgccagcg
     2941 gaaagcaggc cttcaatcac cgccgtgttt ttgcggtcgg caaaccagcg gcaaaccgat
     3001 tcggcgacca ccggaccgat atcggccacc gtctgcaaat cttcaaaaga cgcgctccgg
     3061 atactttcaa tgttcccgaa tttttccgcc aacgcccgcg cggtctgttc gccggcattg
     3121 cggattccca aagcatagat gaattgcggc aggccgatcg tttttttggc gtcaaccgat
     3181 tccaccagat tccgggcgga tttttcgccg aatcttgcca gcggcgccaa atcgccttct
     3241 ttgaggccga aaatatcggc gggggcgcgc accaatcctt ggtcaatcaa ttgctcaata
     3301 atcttttctc ccagcccgtc aatgttgaac gcgccgcggg aaacaaaatg ccgcaaagat
     3361 cttttttgca ccgcaaaaca atcccgattg atacagcgca gaaccgcttc gcccggtttt
     3421 ttttcggttt tcccgccgca catcgggcaa atttcgggca tcttaaacgc tgtttcgtcg
     3481 ccggtgcgca attccggcag gacggcgata attccgggaa tcacgtcgcc ggcgcggccg
     3541 acaatcaccg tgtcgccgat tttcaccccc agacggcgga tttcatcttc gttgtgcagg
     3601 gtcgcgcggg aaatcatcac gccgccgact tttaccgggc gcaaaatcgc caccggcgtg
     3661 agcgcgccgg tgcgccccac ctgcacgaca atgtcttcca cgacggtggt cgcttgtttt
     3721 aaaggaaact tgtaagcgat ggcggcgcgg ggagatttgc cggcgatccc cagtttctca
     3781 aacagatcgt ttcggttcac cgccaccacg atgccgtcaa tctcataggg caggcggccg
     3841 cgatgatcgc gccagtgccg gtaaaagacg aaaacctctt gcatctttgc gcaaaaacgg
     3901 ctgaacttgt tattggtttt gaagcccgca atctccaaaa atcggtgctc ttcctcgtgg
     3961 gttttttgcc ccaaatcggt tactaacttg tagcaatttg aatccaatcg gcgggaggcc
     4021 gcgatcttgg gatccaactg gcggattgat ccggcggcca gattgcgggg gttggcaaat
     4081 tccggcaggc ctttttttct ttgattgtcg ttgatggcgt caaaatcgcg gcgcgacacc
     4141 accacttcgc cgcgcaccgc caagtgccgc catccgccgc gggtaactgc cgcggccata
     4201 tccttcagac cggcggcgcg caaatccgcg gcaatttcct ccgcggcgcg caggcgcagg
     4261 ggaatcgcct caatggtttt caaattgttg gtaacgtttt cgccgacccg gccgtcgccg
     4321 cgcgtggcgc ccgccgccaa aattccgtcc tcgtagatca gttcaatggc caatccgtca
     4381 agcttgggtt cgcaataaaa atcaatccgg gcgcgttcgg cgtcggaaag caagcggccg
     4441 atccgttctt gccagtcaag catatcctgt tttgaaaacg cgtcgtttaa agaaagcatc
     4501 ggcgac
//
LOCUS       DAOQWE010000065         4485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_19743_length_4485_cov_7.415801, whole genome shotgun sequence.
ACCESSION   DAOQWE010000065 DAOQWE010000000
VERSION     DAOQWE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19743_length_4485_cov_7.415801"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="PKK37_02875"
     CDS             <1..808
                     /locus_tag="PKK37_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNR81354.1"
                     /translation="NFLMFNGKKIGLALSGGGALGAAHIGIIEEIEKAGVKPDCVCGV
                     SSGAIIGLIYAAGGTKALHDFFDELAGSKIFQKKEYLLIGGPRRIRELVESLLEKRIA
                     GRSFADLEMNFAGVATNIATGRKEMISRGDPVVAVMASAAYPALSPIQKIGSNYYFDG
                     GVTCNLPAEDARRMGADFVIGSSIYSISEIDRKEVAKMGKLAILWRTFDIFDKQLNRY
                     EARSCDFLFKPKIDSFKIFDFTRLREIEKMGRAHAKRNIKTLVNLLKNEK"
     gene            798..1526
                     /locus_tag="PKK37_02880"
     CDS             798..1526
                     /locus_tag="PKK37_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNR81355.1"
                     /translation="MKNKNPTALGNLREDLRQAANPQKAKILQRFFKTGPGEYAEGDI
                     FLGVAVPQTRAIIKKYWREISIKEAEILLHDKIHEERLAALLILVEKFEKGDEPSRKE
                     IFNLYLANAKFVNNWDLVDLSAPKIVGAYLEDKDKNILLDLAESKNLWERRIAMLATF
                     RFIKNGDAAMALKIAEILINEKEDLIHKAVGWMLREIGKNCGQKIEEDFLRRHYRIMS
                     RTTLRYAIERFDEDTRQTYLTGRI"
     gene            1735..2139
                     /locus_tag="PKK37_02885"
     CDS             1735..2139
                     /locus_tag="PKK37_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNR81356.1"
                     /translation="MKISKKVQYGLRAMAYLAQNSKDNRAVSVREIAIRGKMPNDYLE
                     KVFAKLLKANLVAAKKGVRGGYVMGRTADKIRISEIFNAVENPATKVKCIDAKCPREG
                     ECLAKSLWVKMQAAVNNFLESVSLDDLINKKI"
     gene            2161..3372
                     /locus_tag="PKK37_02890"
     CDS             2161..3372
                     /locus_tag="PKK37_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNR81357.1"
                     /translation="MAKIIVSKKKKVLRNLGPRRIYMDYGAATPVDGRVAKKIAAVLP
                     EIFANSMSFHSFGRQAREVLEQSRAEIASVINAKPEEIIFTGSATESNNLAIKGVMFA
                     NRQKGKHLIISPIEHACVMESAAWLERQGFEVTRLEVDKYGLVNPGDVAAAIRSDTVL
                     VSVMHANNEIGTIEAIEEIGKICRQRGVWFHTDAAQSFGKIPIDVVAMNVDLLTASSQ
                     KIYGPKGAACLYVRQGVKIEPILHGGGQESGMRSSTVNVPAIYGFAQAGLIAKKEMAK
                     EAARQTKMRDKLIAQILGTIPHSYLNGHPQKRLPNNANVRFDFVEGESMIVQLDVNGI
                     AASTGSACSSAKLTASHVLTAIGMAPQEAHGSLRLTIGRGTKESDINYVLKILPGIIQ
                     RLRELSPFKIN"
     gene            3389..3967
                     /locus_tag="PKK37_02895"
     CDS             3389..3967
                     /locus_tag="PKK37_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNR81358.1"
                     /translation="MLKRPKKTITCQPKCGVCPKGRTAASGPYTAGVMEHFSKPHNYG
                     KIKNPDGVGKVGNLICGDVMWLYIKVAKGRAGKEIIKDIKFETFGCVAAIATSSAITD
                     LAKGKTLEEAIAIDKREIVQSLGGLPPIKLHCSVLAADALAEAIYDYYTKQKRPIPAA
                     LEEKHQRIKKEKDIVEEKYKDWVKKEEEIHNQ"
     gene            4093..>4485
                     /locus_tag="PKK37_02900"
     CDS             4093..>4485
                     /locus_tag="PKK37_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNR81359.1"
                     /translation="MVLDLTKLNKEQRQAVEHKDGPLLIVAGAGTGKTRAITYRIAHL
                     IESGDAKPDEILALTFTDKAAGEMEDRVDELLSLAYADAWILTFHAFCERILRRHALD
                     IGVPGNFKVADQTAAWLLARKNLDRFKLK"
BASE COUNT         1269 a          982 c         1217 g         1017 t
ORIGIN      
        1 taatttcctt atgtttaacg gaaaaaaaat tggtttggcg ctttccgggg gcggtgcgct
       61 gggcgcggcg cacatcggca tcattgagga aattgaaaaa gcgggagtga agcccgactg
      121 tgtttgcggc gtgtcgtcgg gtgcgattat cggtttgata tatgccgccg gcggcacgaa
      181 agcgttgcac gatttttttg acgaactcgc cggttccaaa atttttcaaa aaaaagaata
      241 tctgctcatc ggcggcccgc gccggattcg cgagctggtg gaatcattgc tggaaaaaag
      301 gatcgccggc cgcagttttg ccgatttgga aatgaatttt gccggcgttg cgaccaatat
      361 cgccaccggc cggaaagaaa tgatttcccg gggagatccg gtggtcgcgg tgatggcgtc
      421 ggcggcctat ccggcgctgt cgccgataca aaagatcggc agtaactatt attttgacgg
      481 cggcgtgact tgcaatctgc ccgccgaaga cgcccgccgg atgggggcgg attttgtcat
      541 cggctcctca atttattcca tcagcgaaat tgatcgcaaa gaagtcgcca agatggggaa
      601 gctggcgatt ctgtggcgta cgtttgatat ttttgacaag caattgaacc gttatgaagc
      661 ccggagttgc gattttctgt tcaaacccaa gattgattct ttcaagatat ttgatttcac
      721 ccgcctgcgc gagattgaaa aaatgggccg cgcccacgcg aaaagaaaca tcaaaacttt
      781 ggttaatctt ttgaaaaatg aaaaataaaa acccaaccgc gttgggaaat ttgcgcgagg
      841 atttgcgcca agcggccaat ccccaaaaag cgaaaatact gcaaaggttt ttcaagactg
      901 ggccgggcga atatgccgaa ggcgacatat ttttgggagt ggccgtgccg caaacaaggg
      961 cgataatcaa aaaatattgg cgcgaaattt caatcaagga agccgaaatt cttttgcatg
     1021 acaaaatcca cgaagaacgg ttggcggcgt tgttgatatt ggtggaaaaa tttgaaaagg
     1081 gcgatgagcc atcgcgcaag gagattttta atctttattt ggccaatgcg aaattcgtca
     1141 acaattggga tttggtggac ctgtccgcgc caaaaattgt cggcgcgtac ttggaggata
     1201 aagacaaaaa tattttgctt gatttggccg aatcaaaaaa cttgtgggag cggcgcatcg
     1261 cgatgctggc aacattccga tttatcaaaa acggcgatgc cgcaatggcg cttaaaattg
     1321 ccgaaatttt aatcaacgaa aaagaagatt tgattcacaa ggccgtcggg tggatgttgc
     1381 gggagatcgg caaaaattgc ggccaaaaaa ttgaagaaga ttttttgcgc cggcactatc
     1441 ggattatgtc tcgcaccacc ctgcgctatg ccattgaacg ctttgacgag gacactcggc
     1501 aaacctactt gacggggaga atataaattt gctaatataa agccgacact agcagtcggt
     1561 tttataatag acctactgat taaatatttt tgtaacgaaa ttttcagatt tcgcgcgagc
     1621 cgaatgagga agaaggaggc atacttggct taaagtctgc cgacgactga cgagtttggc
     1681 gtagccgaaa tatggaaatt gcagtaggaa atatttaatc agtgggtcta atatatgaaa
     1741 atctccaaga aagtgcaata tggtttgcgg gcgatggctt atttggcgca aaattcaaaa
     1801 gataaccgcg ccgtttcggt gcgcgagatt gccatcaggg ggaaaatgcc caatgattat
     1861 ttggaaaaag tttttgctaa actcttgaaa gcaaatttgg tggcggcaaa aaaaggcgtg
     1921 cgcggcggat atgttatggg ccgcaccgcg gataaaatcc ggattagcga aatttttaat
     1981 gcggttgaaa atccggccac aaaagtgaaa tgcattgacg ccaaatgccc gcgcgaaggc
     2041 gaatgtctgg ccaaatccct ttgggtcaaa atgcaagcgg ccgtcaacaa cttccttgaa
     2101 tccgtgagtc tggatgattt gattaacaaa aaaatttaaa attttacatt tttaaaagtt
     2161 atggccaaaa tcatcgtttc aaaaaagaaa aaagttttgc gcaatcttgg gcctcggcgg
     2221 atatatatgg actatggcgc ggctacgccg gtggacgggc gcgttgccaa aaaaatcgcc
     2281 gctgtgttgc cggaaatttt tgccaattca atgtcgtttc attcattcgg gcggcaggcg
     2341 cgcgaggttt tggaacagag ccgcgcggag attgcgtcgg tgatcaatgc caagcccgaa
     2401 gaaataattt tcaccggctc ggccacggaa agcaataatc tggcgatcaa gggcgtgatg
     2461 tttgccaatc gccaaaaagg aaaacatttg atcatttcgc cgattgagca cgcgtgcgtg
     2521 atggaaagcg cggcgtggct ggagcggcaa gggtttgaag tgacgcgcct tgaagttgac
     2581 aaatacggat tggtaaatcc cggggatgtc gccgcggcca ttcgttccga cacggtgctg
     2641 gtgtcggtga tgcacgccaa caacgaaatc ggcacgattg aggcgattga agaaatcggc
     2701 aagatttgcc gccaaagagg agtttggttc cacaccgacg cggcacaatc attcggcaaa
     2761 attcccattg atgttgtcgc gatgaacgtt gatcttttga ccgccagttc ccaaaaaatt
     2821 tacggtccca aaggagcggc gtgcctgtat gtccgccaag gcgtgaagat tgagccgatt
     2881 ttgcacggcg ggggacagga atcggggatg cgatcgtcca ctgtcaatgt gccggcaatc
     2941 tacggctttg cccaggcggg gctgatcgcc aaaaaggaaa tggccaagga agcggcgcgg
     3001 cagacaaaaa tgcgcgacaa attgatcgcg caaattctgg gaaccattcc ccattcgtat
     3061 ttaaacggcc acccgcaaaa acgactgccc aacaacgcca atgtccgttt tgattttgtg
     3121 gaaggcgaat caatgatcgt ccaattggat gtcaacggca ttgccgcgtc caccggttcg
     3181 gcttgttcat cggccaaatt gaccgccagc cacgtgctca ccgccatcgg catggcgccc
     3241 caagaagccc atggatcatt gcgtctgacc atcgggcgcg ggacaaaaga aagcgacata
     3301 aattacgtgt tgaaaattct gcccggaatc attcagcgac tgcgcgaatt gtctcccttt
     3361 aaaatcaatt gaaatcaaaa caataattat gctcaagaga ccaaaaaaaa cgataacttg
     3421 ccaaccaaaa tgcggcgtgt gtcccaaagg caggaccgcc gccagcggcc cttataccgc
     3481 cggcgtgatg gagcattttt ccaaaccgca taattacggc aaaatcaaaa accccgacgg
     3541 cgtcggcaaa gtcggtaact taatatgcgg tgacgtaatg tggctgtata tcaaagttgc
     3601 caaaggcagg gccggaaaag aaatcatcaa agacattaag tttgaaactt tcggctgtgt
     3661 ggcggcgatc gccacttcca gcgcgatcac ggatttggcg aaaggcaaaa ctctggaaga
     3721 ggcgatcgcc attgacaagc gggagatcgt gcaaagtttg ggcggcttgc cgccgatcaa
     3781 acttcattgc tcggtgctgg cggccgatgc gcttgccgaa gcgatttacg actattacac
     3841 gaagcaaaaa cggccaattc cggcggcgct ggaagaaaaa caccaacgga taaaaaaaga
     3901 aaaagacatc gtggaagaaa aatacaagga ttgggtgaaa aaggaagagg aaatccataa
     3961 tcaatgaaca tcaaacattg aactttgaac tttgacaaat gcaaaaaaaa acaaaaacgt
     4021 tccgcgcgca atcggagcgt ttttgttttt ggcgattttg cggtaaaatg cggaattggg
     4081 attaaaaata agatggtgct ggatttgaca aaactgaata aggagcaacg gcaggcggtg
     4141 gagcataaag acgggccgtt gttgatcgtg gcgggggcgg gcaccggaaa gacgcgagcg
     4201 atcacttatc gcatcgcgca tttgattgaa agcggagatg ccaaacccga cgagattttg
     4261 gcgctaacgt tcaccgacaa agccgccggc gaaatggagg accgcgtgga tgagttgttg
     4321 tcgcttgcct atgccgacgc atggattttg acgtttcacg cgttttgcga gcggattttg
     4381 cgccgccacg cgctggatat tggcgttccc gggaatttca aagtggcgga tcaaaccgcg
     4441 gcgtggctgt tggcgcgcaa aaatttggac cgcttcaagt taaaa
//
LOCUS       DAOQWE010000066         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_19939_length_4457_cov_5.695593, whole genome shotgun sequence.
ACCESSION   DAOQWE010000066 DAOQWE010000000
VERSION     DAOQWE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19939_length_4457_cov_5.695593"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1065
                     /locus_tag="PKK37_02905"
     CDS             70..1065
                     /locus_tag="PKK37_02905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR81360.1"
                     /translation="MDKTNLSIVIVNHNARQLLRQCLESIKNANIKSSHEIIVVDNNS
                     RDGSQKMLKEEFGGVIRILNNENLFFGKANNQGARISRGKYILFLNPDTIVMPDSIDT
                     MVKFLENNPKVWGVGPKLCNFDGTVQYGFYRKFPSIISLLLFYTPLMAISKKSKFLRR
                     NFFEDMDFSKAKQVQQPPGAALLVRREIIDKIGGYFDEDFHIWYEDVDLAFRVKKAGG
                     RLYVVPEAEIRHVGGHSFTPFKMLDRRLQFLSSLKLFLKKHRGALVGKMFFLFVYFGA
                     LVLASYHIITAPFVLGFAFNDFSNKKRLQFEKINDLIRFLFSWQKEWNKENKWLK"
     gene            1053..2957
                     /locus_tag="PKK37_02910"
     CDS             1053..2957
                     /locus_tag="PKK37_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR81361.1"
                     /translation="MAEIIYHKEKLVFALFSLLLFLPALFSGGKDIKAQVIFFILLFP
                     LAFFVWKMNDRFEVSAAKLTFALAAAFVLLAGISAIYSPQHYQALLGFFNLLACLILA
                     LLVFNFADSPLKLKYFGYIILALGAVLSLIGIYDFVLSQNYQSLRLVSTFYSQIPFSE
                     FLIYPFFIALCLLFLSRPAKKERNILIVLNILFGVTLFFTHCRGAGVSFALVLLFLVV
                     FFKKEIINKKSLAFLAAIVIPGVFSIAVIMQVKSWQAHRDSAPAAVYNGPETIQENAV
                     SARLLFWQRAWDIFKDRPLTGGGLASYPELHKQYLQPPFYYSADPHNLYLKILAELGI
                     VAFLVFIGFIASFLRRFLTFTNHLRKTGVAGKDKSSVISLAIMAGIVSALIGNGVGFG
                     WDFPADMIIFFFSLGLALKACAIYSGAVPANPERKIGLIVFPGAAFLFFFAPIIFFAD
                     NNFQDYQYYLQEKDENNALAVLRQARQINPFNPQYSALLASFDLSSGRLDQAQAEISH
                     ALTWSDTNPNLLLQGRIYLAQNRPDLAQAKFKEAIEKYPSGLAAYTVLSVFYQDRNRF
                     SEIPPLLAEVLPLYKKEYILSPLYIASDKNSILNQISALYSINANAYAKLGNDGEAQI
                     QLNAAAEYAQ"
     gene            2947..3795
                     /locus_tag="PKK37_02915"
     CDS             2947..3795
                     /locus_tag="PKK37_02915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR81362.1"
                     /translation="MPSELSIIVNHCRTPELLRNCLESVKTNVRQIDYELIVADSFTQ
                     GQTAVLVKKIIPEAVYLPFFKNVGFGRSVNEAVKKSSGEYLLILNADTALRDKNAVSS
                     MIDCLRADPAIGLLGPRLVNSDGSVQESAFRFYDISALVCRRTVLGKTAFGKKILARF
                     TLKDVLTPANIAKGRPVSVGWLMGSVLLARRSAVAKVGLLDERFFMYFEDVDWARRFW
                     ESGFKVAYFPAASVFHLHLRASRKSRGIFSLFLNRYARIHLSSAVKYLLKYAFRKPPI
                     SIRVCP"
     gene            complement(3773..>4457)
                     /locus_tag="PKK37_02920"
     CDS             complement(3773..>4457)
                     /locus_tag="PKK37_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNR81363.1"
                     /translation="FQTFFTNINAETVAEFPILEFRRTPLEGWGGFVKRLTDAVISGV
                     GIVFLLPVGVIVGLAIKHDSPGPVIVKLKRVSQGKEFYLYKFRSMKNNSHKLKKELME
                     YNQRKDGPLFKMENDPRVTRIGKFLRRTWLDEFPQLFNVFAGEMSLVGPRPHEPEETN
                     RYLNHHKRTFFVKAGMTGLAQINGGCNLNFEEEVKLDLYYIKNWSLARDIKILAKTMI
                     LFFKDKPGC"
BASE COUNT         1063 a         1095 c          918 g         1381 t
ORIGIN      
        1 cgtagccgaa atatggaaat tgcagtagga aatatttacg cagtaggtct aataaactat
       61 ggatggaata tggataaaac caatctttca attgtaatag tcaatcataa tgcgcggcaa
      121 ctgttgcgcc agtgtttgga atcaataaaa aacgccaata taaaatcgtc tcacgaaatt
      181 atcgtcgttg acaacaattc ccgcgatggc agccaaaaaa tgttaaaaga ggaattcggc
      241 ggcgttatcc gtattctgaa caatgaaaat cttttctttg gaaaagccaa caaccagggc
      301 gcgcgaatca gccgggggaa atatattctg tttctgaatc ccgacacgat tgtaatgccc
      361 gattccattg acacaatggt taagtttctt gaaaataatc ccaaagtatg gggggttggc
      421 cccaaacttt gcaattttga cggcaccgtc caatacggtt tttaccgcaa gtttccttca
      481 atcatttcat tgttgctttt ttacactccg ttgatggcaa tttccaagaa atcaaaattc
      541 ttgcgccgca atttctttga agatatggat ttttcaaaag cgaaacaagt tcagcaaccg
      601 cccggcgccg ctctcttggt aaggcgggaa ataattgata aaatcggcgg ctattttgat
      661 gaagatttcc acatttggta tgaagacgtt gatctggctt ttcgcgtcaa aaaagccggc
      721 ggccggcttt atgttgttcc cgaagccgaa atccggcatg ttggcggcca cagtttcacg
      781 ccttttaaaa tgcttgaccg ccgtttgcaa tttttatcaa gtttgaagct atttctgaaa
      841 aaacacagag gcgcgctggt cggcaagatg ttttttttgt ttgtttactt tggcgctttg
      901 gttcttgctt cttaccatat catcaccgct cccttcgtgt tgggatttgc gtttaacgat
      961 ttttcaaata aaaaaagatt gcaatttgaa aaaatcaacg atttaatccg atttttgttt
     1021 tcctggcaaa aagagtggaa taaagaaaac aaatggctga aataatttac cacaaagaaa
     1081 aactcgtttt tgcgcttttt tctctgttgt tgttcttgcc cgctctgttc tcgggcggca
     1141 aagacataaa agcgcaagtt attttcttca tcttattgtt tcccttggcc ttctttgttt
     1201 ggaagatgaa cgataggttt gaagtttccg ccgccaaatt gacttttgcg cttgcggcgg
     1261 cctttgtttt gctggcggga atttcggcca tttattcgcc ccagcattat caagcgcttt
     1321 tgggattttt taatttgctt gcctgcctta tccttgcgct gttggttttt aactttgccg
     1381 attcgccgtt aaaattaaaa tattttggct acatcatcct tgccttgggc gcagttcttt
     1441 ctttgatcgg catctatgat ttcgttctgt ctcaaaatta tcaatcgttg cggctggttt
     1501 ccacttttta cagccagatt cctttcagcg aatttttgat ttatccgttt ttcatcgctc
     1561 tctgcctgct tttcttgtct cgtcccgcga aaaaagaaag aaatatcctg attgtgttga
     1621 atattttgtt tggcgtcacc ctttttttta cgcattgccg cggcgccggg gtaagtttcg
     1681 cgctggtttt gctgtttttg gttgtttttt ttaaaaaaga aatcatcaac aaaaaatctc
     1741 tcgcgtttct ggcggcaatc gttatccccg gcgttttttc catcgccgtt attatgcagg
     1801 taaaatcctg gcaagctcat cgcgattccg ctcccgccgc cgtctacaac ggccccgaaa
     1861 ccatccaaga aaatgccgtt tccgctcgtc tccttttttg gcaaagagcc tgggatattt
     1921 tcaaagaccg tcctttaacc ggcggcggtc tggcttctta tcccgaattg cacaaacaat
     1981 atctccagcc accgttttac tacagcgccg atccgcacaa tttgtatctc aaaattttag
     2041 ccgaactcgg gattgtcgcg tttctggttt tcatcggctt cattgccagc tttctccgtc
     2101 gttttttaac ttttaccaac catttgcgaa aaaccggcgt tgccggcaag gataaatcgt
     2161 ccgtcatttc tctggcgata atggccggca ttgtcagcgc gcttatcggc aacggcgtcg
     2221 gtttcggctg ggattttccg gccgatatga taatattttt cttttctctg gggcttgcgt
     2281 taaaagcttg cgccatctat tccggcgccg tccccgccaa tccggaacga aaaattggcc
     2341 ttatcgtttt ccccggcgcc gcttttctct tttttttcgc gcctatcatt ttttttgctg
     2401 ataataattt tcaagactat caatattatc ttcaagaaaa agacgaaaat aacgcccttg
     2461 ccgttttgcg gcaagcccgt caaattaatc cgttcaatcc tcaatattcc gctttgctgg
     2521 catcttttga tctgtcatcc ggccggctgg atcaagctca agccgaaatt tcccacgcct
     2581 taacttggtc ggatacgaat ccgaatttgc ttctccaagg ccgaatatat ttagcccaaa
     2641 accggcccga ccttgcccaa gccaaattta aagaagccat tgaaaaatac ccctccggct
     2701 tggccgctta caccgtgctg tccgtttttt atcaagaccg gaaccgcttc tccgaaattc
     2761 cgcctctctt ggccgaagtc ttgcctctct ataaaaaaga atatattctc tcgccccttt
     2821 acattgcctc cgataaaaat tcaattctca accaaatttc cgctttgtac tccatcaacg
     2881 ccaatgccta tgccaagctt ggcaacgacg gggaagcgca aattcagtta aacgccgccg
     2941 ccgaatatgc ccagtgaact ttcaatcatc gtgaatcatt gtcggacccc ggaattgttg
     3001 cgaaactgcc ttgaatccgt caaaaccaac gtgcggcaaa ttgattatga attgattgtc
     3061 gccgacagtt ttacccaagg ccaaaccgcg gttttggtta aaaaaataat tccggaagcg
     3121 gtttatctgc cgttttttaa aaatgtcggt tttggccggt cggtgaacga agccgtcaaa
     3181 aaatcaagcg gagaatatct tttgattctc aatgccgaca ccgctctgcg cgataaaaac
     3241 gccgtttcat caatgattga ttgcttgcgc gccgatcccg ccatcggatt gttggggccg
     3301 cgcttggtga attcggacgg ctccgttcag gaatccgcat ttcgtttcta tgatatttcc
     3361 gcccttgttt gccgccgtac cgttcttggc aaaaccgctt tcggtaaaaa aattcttgcc
     3421 agattcactt taaaagacgt tttgactccc gccaatattg caaaaggccg gccggtctcc
     3481 gttggctggc tgatgggatc ggtgcttttg gcccgccgtt cggccgttgc caaagtcggg
     3541 cttctggatg aaagattttt tatgtatttt gaggacgttg attgggcgcg ccgcttttgg
     3601 gaaagcggtt ttaaagttgc atattttccc gcggcgagcg tttttcatct gcatcttcgg
     3661 gccagccgca aatcccgcgg cattttttca ttattcttaa accgttacgc ccgaattcat
     3721 ctttccagcg ccgttaaata tctgttaaaa tacgcctttc ggaaaccccc gatcagcatc
     3781 cgggtttgtc cttaaaaaat aatatcatcg tcttcgccaa aatcttgatg tcccgggcca
     3841 gcgaccagtt ttttatgtaa taaagatcaa gtttgacttc ttcttcaaaa ttcaaattac
     3901 agccgccgtt tatttgcgcc aatcccgtca tgcccgcttt cacgaaaaat gtccgcttgt
     3961 gatggtttag atatctgttg gtttcttccg gttcgtgcgg ccttggcccc accaggctca
     4021 tttcgccggc gaaaacatta aacaattgcg gaaattcgtc cagccatgtc cgccgcaaaa
     4081 atttgccaat ccgcgtcacc cgcggatcgt tttccatttt aaaaagcggc ccgtcttttc
     4141 tttgattgta ttccatcagc tcttttttca atttgtggga attgtttttc attgagcgga
     4201 atttatacaa ataaaattct tttccctggc tcaccctttt taatttcaca atcaccggcc
     4261 ccgggctgtc gtgttttatg gccaggccga cgataactcc gacggggagt aagaaaacaa
     4321 ttcctacccc cgaaatcacc gcatccgtta accttttgac gaagccgccc cagccttcca
     4381 gcggcgtacg cctgaattcc aaaatcggaa attccgccac cgtctcggcg ttgatattgg
     4441 tgaaaaaagt ctggaaa
//
LOCUS       DAOQWE010000067         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_20644_length_4345_cov_8.167133, whole genome shotgun sequence.
ACCESSION   DAOQWE010000067 DAOQWE010000000
VERSION     DAOQWE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20644_length_4345_cov_8.167133"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKK37_02925"
     CDS             <1..528
                     /locus_tag="PKK37_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR81364.1"
                     /translation="SYSILGKHLLVLFQWFHVLSKAIHWKEKRGLAPDYFPKRLPDKL
                     AVLALNQFKKLEKFNEHRRKIAELYYNKLKGGDCVLPARPSGRKAVYLRFAIRRDCAA
                     KMIKKAWQKNLLLGDWYRTPVDPFDTLPETMGYKKGSCLNAEAFAGKIINLPTHINIG
                     ESEARKILTFIKQCK"
     gene            528..1589
                     /locus_tag="PKK37_02930"
     CDS             528..1589
                     /locus_tag="PKK37_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNR81365.1"
                     /translation="MDIESRKEWEGILEKCEDKTFLSSWEWGEFWAGQGNKIKRLGVG
                     DKSGLKAIVLAVKIKARRGTYILVQHGPNIIETNPEEIKKILKELFFGLANWGKSEGA
                     SFIRCAPMLARTEENLTLLKNLRFPEFRFLLFQSRFLISPMHANAYEATWKLDIMPPE
                     EDLMKAMRKTTRYLIRQVQKNPEVKIEMSDKPEDIEIYQKLNEAVAKRQKFSAFGRDY
                     IKREFETFAKNGRARWFVGKYKNKPVCAALVIFWSGIGFYHQAASLGEYSKYSIPYLL
                     QWEAIKEAKRRGCGLYDFWGYTDPKKYPKHPWAGPTLFKMGFGGEKYEYVKTMDLPLS
                     WKYWPTRLFEKVRKLKRGL"
     gene            1719..2549
                     /locus_tag="PKK37_02935"
     CDS             1719..2549
                     /locus_tag="PKK37_02935"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNR81366.1"
                     /translation="MVRRTYKKPHRARRKKPLWVKAGFWRAIFLTGAGGFTIWLVCFS
                     PVFEVKEIEIAGAQKIEEREFVDVIEKSVNKRIGLFDSKSILLFNLDQTKRELLARLP
                     QAQEIKIEREFPSKIIASVQERRAAAVFESQGKLYSLDEAGVAFEECASAAGLIKILD
                     GSMAVEIGAKAIEKEVLNGILRITSELRASTDIACEEAIIASPERINLPVKEGWQIYF
                     NPRKDAESQIIRLLAVIKEDSFREKRNNLEYLDVRFTRVYLKEKNTDPAEEKAEETVD
                     "
     gene            2578..3309
                     /locus_tag="PKK37_02940"
     CDS             2578..3309
                     /locus_tag="PKK37_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNR81367.1"
                     /translation="MGFYKRQPYIPGAKAPFALSRSKVDMFLKCPRCFYLDVRLGIKT
                     PSMPAFTLNSAVDHLLKKEFDIRRANGAIHPLMAAYGIDAVPAQHDELEIWRENFKGI
                     RVLHPKTNFELFGAIDDLWINPKGEYHIVDYKATSTVKEIDLNDHWKKWYKIQAEFYQ
                     WLLRVRNFTVSDTAYFVFANAGKDREAFDGKLEFQVTIVPYEGNDAWVEETLAKMKEC
                     LDADNPPAPTPDCEHCQYIEKAKAI"
     gene            3368..4345
                     /gene="mltG"
                     /locus_tag="PKK37_02945"
     CDS             3368..4345
                     /gene="mltG"
                     /locus_tag="PKK37_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNR81368.1"
                     /translation="MIKQALYVFLAAILLAAATGAVFELEGKSRQPQETITILRGQNA
                     PQIATDLKSEGYIKSKLRFLFYLARDGHWRNLKSGEYDLAGLDDRQIIEKMVSGQTAP
                     KIATIIPGWTVADIQKSAGIAKVLPDADFGAVDVEAFKGKFDFLREIPAGGDLEGYLF
                     PDTYQLPENASIDDLITPALENFSAKLDLLGREKIAAKNLSVRDTVIMASILEKEVRT
                     LEDKKIAAGILWKRLGAGMPLQTDATLLYYKAGSPDHINKDLDSPYNTYEHTGLPPGP
                     ICNPGFDSLEAAIEPVQTDYWYYLSAADGATVFSKTYDEHLQNIARYLE"
BASE COUNT         1244 a          898 c         1158 g         1045 t
ORIGIN      
        1 tcatattcaa ttttgggaaa gcatctgctt gttctttttc aatggtttca tgtcctgtca
       61 aaagccattc attggaaaga aaagcggggg ttggcaccgg attatttccc caagcggttg
      121 ccggacaaac tggcggtttt ggcgctcaat caattcaaaa aactggaaaa attcaacgag
      181 caccggcgca aaattgcgga attgtattat aataaattga agggcggcga ttgcgtttta
      241 ccggcgcggc caagcggcag aaaagcggtt tatttgcggt tcgcgatccg gcgcgattgc
      301 gcggcaaaaa tgataaaaaa agcgtggcaa aaaaacctgc ttttgggcga ctggtatcgg
      361 acgccggtgg atccgtttga caccttgcct gaaaccatgg gttataaaaa aggcagttgt
      421 ctcaacgccg aggcatttgc cggcaaaata atcaatctgc cgacccatat caatatcggg
      481 gaaagcgaag caagaaaaat tctaaccttt atcaagcaat gcaaataatg gatattgaaa
      541 gcaggaagga gtgggagggg attttggaaa aatgcgaaga taaaacgttt ctctcctcat
      601 gggaatgggg ggagttttgg gcggggcaag ggaacaaaat caagcggctg ggggttgggg
      661 ataagtcggg gttgaaagcg attgtgctgg cggttaaaat aaaagcgaga cgaggcactt
      721 atatcctggt acagcatgga ccgaacataa ttgaaacaaa tccggaggaa ataaaaaaga
      781 ttctcaagga attatttttt gggttggcaa attggggtaa aagcgagggc gcgagcttca
      841 ttcgttgcgc tccaatgctg gcgcgaaccg aagagaatct gacattgctt aaaaacttgc
      901 ggtttcccga attccggttt ttgctttttc aatctcggtt tttaatttct ccgatgcatg
      961 ccaatgctta cgaagcgaca tggaaactgg acattatgcc ccccgaagaa gacttgatga
     1021 aggcgatgcg caaaacgaca agatatttaa tccggcaggt gcagaaaaat ccggaagtta
     1081 aaattgaaat gagcgacaag cccgaagata ttgagattta tcaaaaatta aacgaggcgg
     1141 tagccaagcg gcagaagttc tcggcgttcg ggcgtgatta tatcaagcgc gaatttgaaa
     1201 ctttcgccaa aaacggccga gcgcggtggt ttgtcggaaa atataaaaac aaaccggttt
     1261 gcgcggcgtt ggtaatattt tggtcgggaa tcggttttta tcatcaggcc gcgtcgttgg
     1321 gcgaatattc caaatattcc ataccttatc ttttgcagtg ggaagcgatc aaagaggcca
     1381 agcgccgcgg atgcggatta tatgattttt ggggctatac cgatccgaaa aaatatccga
     1441 agcatccctg ggcgggccca acgctgttta aaatgggttt cggcggagaa aaatacgagt
     1501 atgttaaaac gatggatttg ccgctgtcgt ggaaatattg gcccacccgc ttatttgaga
     1561 aagtcaggaa attgaaaaga ggcctgtagt tataaattta aaatgtaaaa atgaaaaagt
     1621 aaaaactaaa attaaaaaat taaaattaaa tgcgtgttat catattttaa attttagatt
     1681 gccgttttta aattttagtt cttacttttt aatttgaaat ggttcggaga acctataaaa
     1741 aaccgcatcg ggcgagaaga aaaaaaccgc tttgggtgaa agccggtttt tggcgtgcga
     1801 tttttctgac gggcgccggg ggattcacaa tctggctggt ttgtttttcg ccggtgtttg
     1861 aagtgaagga aattgaaatt gccggggcgc aaaaaataga agaacgggaa tttgtggatg
     1921 tcattgaaaa gagcgtgaac aagcgaatcg gcctttttga ttccaagagc attttgcttt
     1981 tcaatcttga tcaaacaaaa cgggaattgc tggcgcgcct gccgcaggcg caggaaatca
     2041 aaattgaaag agagtttccg tcaaaaatta tcgcgtcggt gcaggaacgc cgcgcggcgg
     2101 cggtgtttga aagccaggga aaactttatt ctttggatga ggcgggagtg gcatttgaag
     2161 aatgcgcctc tgccgccggt ttgataaaaa tccttgacgg ttcaatggcg gtggagattg
     2221 gcgccaaagc gattgaaaaa gaagttttaa atggaattct gcgcattacc agcgaattgc
     2281 gcgcttccac cgacatcgcg tgcgaagagg cgattatcgc cagtccggaa agaatcaatc
     2341 tgccggtcaa agagggctgg caaatctatt tcaatccccg caaagacgcg gagagccaaa
     2401 ttatcaggct gttggcggtg atcaaagaag actctttccg ggaaaaaaga aacaatctgg
     2461 aatatcttga cgtgcgcttt acccgcgtct acttgaaaga aaaaaacacc gatccggcgg
     2521 aggaaaaggc ggaagaaaca gttgactgaa cccgccgcgg cggaattacc aaaaattatg
     2581 ggattttaca aaagacaacc ctatattccc ggcgccaaag cgccgtttgc gctgagccgt
     2641 tccaaagtgg atatgttttt gaaatgtccg cgctgttttt atttggatgt gcgtttgggc
     2701 atcaaaacgc cgtcaatgcc ggcgttcacg ttaaacagcg ccgtggatca tttgctcaag
     2761 aaagaatttg acattcgccg cgcgaacggc gcgattcatc cgttgatggc ggcctatggc
     2821 attgacgcgg tgccggcgca acacgacgaa ctggaaatct ggcgcgagaa tttcaagggg
     2881 atccgggttt tgcatccgaa aaccaatttt gaacttttcg gcgcgattga tgatttatgg
     2941 ataaacccaa aaggggaata tcatatcgtt gattacaagg ccaccagcac cgtcaaagaa
     3001 attgatttaa acgaccactg gaaaaaatgg tataaaatcc aggcggaatt ctatcaatgg
     3061 ctcctgcgcg tgcggaattt caccgtcagc gacaccgcct attttgtgtt tgccaatgcc
     3121 ggcaaagacc gagaggcgtt tgacggcaaa ttggaatttc aggtaacaat cgtgccctat
     3181 gagggcaatg atgcatgggt ggaagaaacg ctggcaaaaa tgaaggaatg tcttgatgcg
     3241 gataacccgc ccgcgccgac accggactgc gaacactgcc aatatattga gaaagccaaa
     3301 gcgatttaga cttgtccata gaatattccg ggccagagtc cgggctattc cggcggatga
     3361 ataaaaaatg atcaagcagg cgttatatgt ttttttggcg gcgatattgc tggcggcggc
     3421 aaccggagcg gtttttgagt tggagggaaa atcgcgtcag ccgcaagaga cgatcacgat
     3481 tttacgcggc caaaacgcac cgcagatcgc cacggacttg aagtccgagg gctacatcaa
     3541 gagcaagctg agatttttgt tttatctggc gcgggacggc cactggcgca atttaaaatc
     3601 gggagaatat gatctggccg gattggatga tcgtcaaatc attgaaaaaa tggtttccgg
     3661 gcaaaccgcg ccgaaaatcg ccacgattat tcccggatgg accgtggccg acattcaaaa
     3721 aagcgccggc attgccaagg ttttgccgga cgctgatttt ggcgccgttg acgttgaggc
     3781 gttcaaaggc aaatttgatt ttttgcggga gattcccgcc ggcggagatt tggagggata
     3841 cctttttccc gacacctatc aattgccgga aaacgcctca attgacgatc tgataacgcc
     3901 ggcgctggaa aatttttccg cgaagttgga tttgctgggc cgggaaaaga tcgccgcgaa
     3961 aaatctgtcc gtgcgcgata cggtgattat ggcctcaatc ctggaaaaag aggtgagaac
     4021 cttggaagac aaaaaaatcg ccgccggcat tttgtggaag cggttgggcg cggggatgcc
     4081 tctgcaaacc gatgcgactc tgctttatta caaggccggc agtccggacc atattaacaa
     4141 agatttggat tcgccctata atacatacga gcataccggt ttgccgccgg ggccgatttg
     4201 caatcccggg tttgactccc ttgaagccgc gattgaaccg gttcagaccg attattggta
     4261 ttatctttcc gccgccgatg gtgccaccgt tttttccaaa acctatgacg aacatttgca
     4321 aaacatcgcc cgctaccttg aataa
//
LOCUS       DAOQWE010000068         4048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_22677_length_4048_cov_3.449036, whole genome shotgun sequence.
ACCESSION   DAOQWE010000068 DAOQWE010000000
VERSION     DAOQWE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22677_length_4048_cov_3.449036"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="PKK37_02950"
     CDS             <1..1672
                     /locus_tag="PKK37_02950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81369.1"
                     /translation="AQIRKLKKNNLGLVFEDKEENVVEQSKVKVPVLKEATKNRIASN
                     NQTPNNLMIEGDNYLALSVLNYTHKKNIDLIYIDPPFNTGARDWKYNNDYVDSEDTYR
                     HSKWLSFMNHRLKLAKDLLKDTGFIACAIDHYELFTLGVLMDKIFGEQNRIGVVTVVH
                     KPEGRQFANFLSPSNEFMLIYAKNRQVASFRDVVLDKEKEKEFNLSDEKGNYKLKNFI
                     RLTDGKYSLRENKPEGFYPIYVSKDLKQFSLEEKTGFEKSLPITDKGAERVWKTFGDT
                     FMEKVKDDAIVAKRENGKIVLYEKLRPSQVFVTHWIKKDYHGYHFGTKVLEDILGSAK
                     QFDFPKSLNLIIDIVKLMAKKDAVVLDFFAGSGTTGHAVLEMNKIDGGNRKFILCTNN
                     ENNNGGNGGIAESVCYPRVKAVINGYKNKKGEKVTGIPSNLFYYKTDLVDIEQIHKVS
                     DDAKIRITYEAGEMIAVREDTLNEIEKNDWWQIFEGNGKLTAIYFKEDKAKLNELIAK
                     LEKKNLPVALYIFSWGKNEYKGEYSSANIRVEDIPEPIIEVYKELNRL"
     gene            1677..3968
                     /locus_tag="PKK37_02955"
     CDS             1677..3968
                     /locus_tag="PKK37_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81370.1"
                     /translation="MFALKPFQETAISQLKDQFLSLWKLANHNIPLVFKSPTGSGKTI
                     MLAQFLRDIVSDPRFAGNDVAFVWFSFSEDSYEQSKKKLFDYYGGASELDLIDLNDLS
                     REKLHQNDVFFINWQKIKGKSKDSRKLRRENEQGLTFDNFINETHEAGRKIVVIIDEE
                     HIGSDTDLAMEVVDGLIKPKITLRVSATPKYIPTRAETAGYVEVKRDDVVEAGLIKEK
                     IVFQTEEDLNQKAVKKLDQDEMLLELAYSKRQELIDLYKKLNVEVNPLVLIQLPNDDQ
                     ASKETSDTTKQTIVLEYLKHKGIKSDEIAVWLSKEKENLEDLEKHTSPVSFLLFKQAA
                     ATGWDCPRASVLVMFREIKNPTFAIQTVGRILRMPFGIHFANPELNLGYLYTNYKRNE
                     VLAEYAKSKTENRPAINGSFRKKNVEPIKLESVFMTRADYNDLGDTFQITFKQVADKK
                     LDTKKLNLKPKVTNGLIVGVEIDDYDNFTKELFEEGGSYDEEMSRHDLERLYNLLCFK
                     LIAKQTDENRKFAPERSWGKLKTALNIYLMEKLKLSRADAYKIIVNDLVGEAGILAPI
                     IGDALLAYRPIREQEVNRKAARAKRVEHIEIPREALFFTDQYEKLAVKKSAMEPFWFE
                     KTQSGLLGGNDNEKKFIDFLESPQNKNVVWWHKNGDNGSEHFSISYYNPDENKEKLFY
                     PDWIIKTKNGVLIVDTKAGITAESNDTRYKAEALQAWLKGRKEFDGGIAVQDGPNGWK
                     INRNAKYSYDSSMKGWEVLDLKN"
BASE COUNT         1437 a          698 c          870 g         1043 t
ORIGIN      
        1 agcccaaatc aggaaactca agaaaaacaa tcttgggttg gtttttgagg acaaagaaga
       61 aaatgttgtt gagcaatcca aagttaaagt gcctgttcta aaagaagcga caaaaaatcg
      121 tatcgcctca aataatcaaa caccaaacaa cctaatgatt gaaggcgaca actatcttgc
      181 gctttcggtt cttaactaca cacataaaaa gaacattgac cttatttata ttgatccacc
      241 attcaataca ggcgcaaggg attggaaata taacaatgac tatgtagata gcgaagacac
      301 atataggcat agcaaatggc tatctttcat gaatcacagg ttaaaactag caaaagattt
      361 gttgaaagat acaggattta ttgcgtgtgc cattgaccac tacgaattat ttaccctggg
      421 tgttttaatg gataaaattt ttggtgaaca aaatcgaatc ggagttgtaa cggttgttca
      481 taaaccagaa ggaaggcagt ttgcaaactt tctcagtccg tccaacgaat ttatgctcat
      541 atacgcaaaa aatcgacaag ttgcttcttt tcgtgatgtt gttttagata aagaaaaaga
      601 aaaggaattt aatctaagcg acgaaaaagg aaattacaag cttaaaaatt ttatccgtct
      661 aacagatggg aaatatagct tgagggaaaa taagccagag ggtttttatc caatttatgt
      721 cagtaaggat ttaaagcaat tcagcttaga agaaaaaact ggttttgaga aatctttgcc
      781 aatcacagat aagggagcgg aaagagtatg gaaaactttt ggcgatactt tcatggagaa
      841 agtaaaagat gatgctattg tggcaaaaag agaaaatggg aaaattgtat tgtatgaaaa
      901 attaagacca agccaagttt ttgtcactca ttggataaag aaggattatc atggatatca
      961 tttcggaaca aaagttttag aagatatttt aggatcagca aaacaatttg atttcccaaa
     1021 atcccttaat ttaataattg atattgttaa gctaatggcg aaaaaagacg cggttgtttt
     1081 agactttttc gctggttcgg gaacaacggg acacgccgtt ttggaaatga ataaaattga
     1141 cggcggaaat cgcaaattta ttctttgtac taacaacgaa aataataatg gcgggaacgg
     1201 cggaattgcc gaatcagttt gctatccgcg cgtcaaagcg gttatcaatg gctacaaaaa
     1261 taagaaaggc gaaaaagtca ctggtattcc tagcaatctt ttctattaca aaaccgacct
     1321 tgtagatatt gagcaaatcc acaaagtatc cgatgacgca aaaatccgca taacctatga
     1381 agcaggcgaa atgatcgccg tgcgcgagga tacgctcaat gaaattgaaa aaaatgattg
     1441 gtggcagatt tttgaaggta acggaaaatt gacggcgatt tacttcaagg aagataaggc
     1501 aaaacttaat gagcttatcg ccaaactgga aaagaaaaat ctgccagtcg ccttgtatat
     1561 tttcagctgg ggcaagaacg aatacaaagg tgagtattcg tccgcgaata tcagagtcga
     1621 agatattccc gaaccgatta tcgaagttta taaagaattg aatcgtttat aaaattatgt
     1681 ttgcactaaa accatttcaa gaaacagcaa tatcacagct caaagatcaa tttttgagtt
     1741 tatggaaatt ggcgaatcac aatatcccgc tcgttttcaa atcgccgacc ggaagcggta
     1801 aaacgataat gctcgcgcag tttttgcgcg atattgtgag cgatccgcgc ttcgcgggca
     1861 acgatgttgc ttttgtttgg ttttcattta gcgaggattc atacgaacaa agcaaaaaga
     1921 aactttttga ttattacggc ggagccagcg agcttgattt aattgacctc aatgaccttt
     1981 cacgagagaa acttcaccag aacgatgtct ttttcataaa ttggcagaaa attaaaggta
     2041 aaagtaaaga tagccgaaaa ctgcgccgtg aaaacgagca gggattaact tttgataatt
     2101 ttatcaacga aacacacgaa gcgggacgca aaatcgtcgt gattattgat gaagagcata
     2161 tcgggagcga cacagatttg gcaatggaag tggtggacgg acttataaag ccaaaaatta
     2221 cactccgtgt ttcggctacg ccgaaatata tccccacccg agccgaaact gcgggatatg
     2281 ttgaagtaaa acgagatgat gtcgttgagg ctggattgat aaaggaaaaa attgtattcc
     2341 aaacagaaga agatttgaat caaaaagcag tcaaaaaact agatcaagat gagatgcttt
     2401 tggaattagc atattcaaaa cgacaggaat tgattgacct gtataaaaag ctcaatgttg
     2461 aagtgaatcc tcttgttttg attcagctac ctaatgatga tcaagcgagc aaagaaacaa
     2521 gtgacacgac caaacaaacg attgtattgg aatatctaaa acacaaaggg ataaaaagcg
     2581 atgaaatcgc tgtttggctt tctaaagaaa aagaaaattt ggaagatctg gaaaaacata
     2641 cttcgccagt atctttcttg ttgttcaaac aagccgccgc gactggctgg gattgtccgc
     2701 gcgcaagcgt gttggtgatg ttccgcgaaa tcaaaaatcc gacttttgca atccaaaccg
     2761 tcgggcgcat tttgcgtatg ccgtttggca tacactttgc caatccagaa cttaatcttg
     2821 gttatcttta cacaaactat aaacgcaatg aagttttggc ggaatacgca aaatcaaaaa
     2881 cagaaaatcg accagcaata aatggaagtt tcagaaagaa aaatgttgag ccgataaaat
     2941 tggaatctgt ttttatgacg cgcgcggact ataacgactt gggcgataca tttcaaatta
     3001 cattcaaaca agtagcggat aaaaaacttg atacaaaaaa actcaatcta aaaccgaaag
     3061 taaccaacgg cttgattgtc ggtgtggaaa ttgacgatta tgacaatttt acaaaagagc
     3121 tttttgaaga aggtggaagt tatgatgagg aaatgtctcg ccatgatctt gaaagattat
     3181 acaatttgct ttgcttcaaa ttgattgcaa agcaaacaga cgagaatcga aaatttgcgc
     3241 cagaacgctc atggggaaaa ctcaagaccg cgctcaatat ctacctaatg gagaaattaa
     3301 aactttctcg agctgacgct tacaaaatta ttgtgaatga tttagtgggc gaagcgggca
     3361 tactggcgcc aattatcggc gacgcgttgt tggcttatcg tccaattcgt gagcaagaag
     3421 tgaacagaaa agcggcgcgc gcaaagcgtg ttgaacatat tgaaatcccg cgcgaagcgt
     3481 tatttttcac tgaccaatac gaaaaattgg cagttaagaa atccgcaatg gagccgtttt
     3541 ggtttgaaaa aacccaatca ggattattgg gtggcaatga taacgagaaa aaatttattg
     3601 attttcttga atcgccacag aataaaaatg ttgtgtggtg gcataaaaac ggcgataatg
     3661 gtagcgagca tttttcaatt tcctactata atcctgacga gaacaaagaa aaattatttt
     3721 atcctgattg gataatcaaa accaaaaacg gcgttttgat tgttgataca aaagcaggca
     3781 ttaccgccga gagcaatgat acaagataca aagcggaagc tctgcaagcg tggctgaaag
     3841 gtagaaaaga atttgacggc ggtattgccg ttcaagacgg accaaatggc tggaaaatca
     3901 accgcaatgc taaatattca tacgatagtt cgatgaaagg ttgggaagtt cttgatttga
     3961 aaaattaagg tggcgcgcca gttttcaact ggcacgaaat tcggaagcga aaaaggaaag
     4021 gttcaaaatc ccgcccgcat tcctcccc
//
LOCUS       DAOQWE010000069         4028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_22816_length_4028_cov_8.683111, whole genome shotgun sequence.
ACCESSION   DAOQWE010000069 DAOQWE010000000
VERSION     DAOQWE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22816_length_4028_cov_8.683111"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..745
                     /locus_tag="PKK37_02960"
     CDS             104..745
                     /locus_tag="PKK37_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81371.1"
                     /translation="MEEAKEILEKLNGLTKAYAKTDEKIDRLAAQTDEKIGGLARLVQ
                     QTDEKIDRLASQTDAKIAEMGKQTDAKIVEMGKQTDAKIAEMGKQTDAKIVEMGKQTD
                     AKIAVFTDEIKRHFGVLVENLDDQIKLIAEGHMDTNRRLDGIENRLDVVEEKVDFISE
                     KTVANSQDLEIVKKDLMFIKQDLKRKVDYDEFAALEKRVLALESRAQKERVIA"
     gene            942..2402
                     /gene="nusA"
                     /locus_tag="PKK37_02965"
     CDS             942..2402
                     /gene="nusA"
                     /locus_tag="PKK37_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNR81372.1"
                     /translation="MDLKSFASAVNQLAQEKGLAPEKIIESIETALAAAYKKEYGEKS
                     QVIKAKLDPKTGQADFWQVKTAMDDSMIFSEEELEAMKNAEALPQKDEKPAAAPEGAP
                     SSESQTAIPEKTRFNPEKHIMIEDAKKDYPDILPGDEIKIELEQKQDFGRIAAQTAKQ
                     VIIQKIREAEKETIFADFKLKEGEIISAVVQRIEGRAAYMDIGKTTGILNREEQVPGE
                     FYRPGQRLKAFVLKVEQTSKGPIIFLSRAYPKFVSKLFELEVPEISTGQVVVKSIARE
                     PGSRTKIAVASMMSEIDPIGAAVGQRGTRVSAVINELGGEKIDIVEYSDETQKYITNA
                     LSPAKIMEVKVLPKNRALAVVPDDQLSLAIGKDGQNVRLAAKLTGWKIDVRSPENLGE
                     ALAQIERENETGNALEEVAPAGDAAGEEEIPQTVSAEPESETCQDEESAPAPEELQGD
                     PEKEETAGKKSKTAKKSVVKTKKTTAAKTKKNKKDE"
     gene            2395..3282
                     /locus_tag="PKK37_02970"
     CDS             2395..3282
                     /locus_tag="PKK37_02970"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNR81373.1"
                     /translation="MNKYTVKKLPKSQVEILAEIPADGFAEFAQRALDEIAKSAEIPG
                     FRKGTAPKEMVKAKIGEQKILDRAAVLAIDDSFPKAAAENNIEPLGYPQISILKLAPG
                     NPFEYKAIAAVYPQTKLPDYKRIAADLEFKAPQVSAEDIKRLQMEKERHARQHWRDDL
                     LEKLVKESELEIPDVLAAGETQKAMEDFKDRVPKMTGMDFAEYLKKIGKTEDQVQEDI
                     AKDSETRIKKFLILREIIKAEKIEVGDEEIAAAIAKSRGEDGENAGDAENEEQEKAYW
                     RQSLQSEKAFEFLEASSKK"
     gene            3427..4011
                     /gene="clpP"
                     /locus_tag="PKK37_02975"
     CDS             3427..4011
                     /gene="clpP"
                     /locus_tag="PKK37_02975"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNR81374.1"
                     /translation="MNLIPTVIEKTQYGERAYDIYSRLLKERIVFLGGPVNDVIANSI
                     IAQFLFLASKDDKKDIQLYINSPGGSVTAGLAIYDTMQYIKCPVSTVCVGMAASMGAV
                     LLASGAKGKRFALPNAEIMLHQVAGGAQGVATDIEINARQIIRIKESLNNIIAKHTGQ
                     KIEKITVDTDRDFYLTAESAKEYGIIDEVIKAKG"
BASE COUNT         1248 a          869 c         1091 g          820 t
ORIGIN      
        1 ttgtcttgcg ttttccttca gacattcggg aaaagtattt tgggaaaagt ttttttgaca
       61 agatttcggc ggaaatggta gactcaagtt attagacaaa attatggaag aggcaaagga
      121 aattttggaa aaattaaacg ggttgacgaa ggcgtatgcc aaaaccgacg agaagattga
      181 tcgtctggcg gcgcagaccg acgaaaagat cggcgggttg gcaaggttgg tgcaacaaac
      241 cgacgagaag attgatcgtt tggcatcaca aacggatgcc aagattgctg aaatgggcaa
      301 gcagactgac gccaagattg tcgagatggg caaacaaacg gatgccaaga ttgccgagat
      361 gggcaaacaa acggatgcca agattgtcga gatgggcaaa caaacggatg ccaagatcgc
      421 ggttttcacc gatgaaatca agcgtcattt cggcgtgctg gttgaaaatt tggatgatca
      481 aatcaaattg attgccgaag ggcatatgga caccaaccgg cgactggacg gcattgaaaa
      541 tcggctggat gtcgtggaag aaaaggttga ttttatcagc gaaaaaacag tggccaattc
      601 gcaggatttg gaaattgtca aaaaagatct gatgtttatc aagcaagatc tcaagcgcaa
      661 ggtggattat gatgaatttg cggcgctgga aaagcgggtt ttggcgctgg aaagccgcgc
      721 tcaaaaagag cgggtgatcg cgtgatttga cacataaagg aggcggtgtt atgattggga
      781 cagtccgatg aaaatcggac attattttta aatttaaaat gtaaatatca aaaatcaaaa
      841 tgacaattta aaatgtaaaa ttacgcagtt tctgaatttg ctaattttta attttgcatt
      901 gtaattttta attttgattt ttaaatttta catttactat cgtggatctc aaatcattcg
      961 cgtcggcggt taaccagctg gcgcaagaaa aagggttggc gccggaaaaa atcattgaaa
     1021 gcattgaaac cgcgctggcg gccgcctaca aaaaagaata tggcgaaaaa agccaagtca
     1081 tcaaggccaa acttgatccc aagaccgggc aggccgattt ttggcaggtc aaaaccgcga
     1141 tggacgattc aatgattttt tccgaggaag aattggaagc gatgaagaac gcggaagcat
     1201 tgccgcaaaa agacgaaaaa ccggcggcgg cgccggaagg cgcaccgtct tcggaaagcc
     1261 agactgcgat tccggagaaa acccgattta atccggaaaa gcacattatg attgaagatg
     1321 ccaaaaaaga ttatcccgac attctgcccg gagacgagat taaaattgag ctggagcaaa
     1381 aacaggactt tggccgcatt gccgcgcaaa ccgccaagca ggtgatcatc caaaaaatcc
     1441 gcgaagcgga aaaggaaacg atctttgcgg atttcaaact caaggaaggg gaaatcattt
     1501 cggcggtggt gcaaagaatt gaggggcgcg cggcctatat ggatatcggc aagaccaccg
     1561 gtattttgaa ccgcgaagaa caggtgcccg gcgaatttta ccgtccgggc cagcggctga
     1621 aagcgtttgt gcttaaagtg gaacaaactt ccaaggggcc gattatcttt ttatcgcgag
     1681 catatcccaa attcgtgtcc aaattgtttg aactggaagt gccggagatc tccacgggac
     1741 aggtggtggt gaagtccatt gcccgcgagc cgggttcgcg cactaaaatc gcggtggcgt
     1801 ccatgatgtc cgagattgat ccgatcggtg cggccgtggg acagcggggg acgcgcgtga
     1861 gcgcggtgat caacgagctg ggcggagaga agattgatat cgtggaatat tccgacgaaa
     1921 cgcaaaaata catcaccaac gcgctgtcgc cggcaaaaat catggaagta aaagttttgc
     1981 ccaagaaccg cgccttggct gtggtgcccg acgatcagct gtcgctggca atcggcaagg
     2041 acggtcaaaa cgtgcggctg gccgccaagc tcaccggatg gaagattgat gtgcgcagtc
     2101 cggaaaattt gggcgaagcg ctggcgcaga ttgaacggga aaacgaaacc ggcaatgctt
     2161 tggaagaagt ggcgccggcc ggtgatgccg ccggcgaaga agaaatcccg caaacggttt
     2221 cggccgaacc agaaagcgaa acctgccaag acgaagaatc ggcgccggcg ccggaggaac
     2281 tccaaggcga tcccgaaaaa gaagaaacgg cgggcaagaa aagcaaaacc gccaaaaaga
     2341 gcgtcgtcaa gaccaagaaa acgacagcgg caaaaaccaa gaaaaacaaa aaagatgaat
     2401 aaatataccg tcaaaaaact tcccaagtcg caagttgaga ttctggcgga gattcccgcc
     2461 gacggctttg cggaatttgc gcaaagggcg ctggatgaaa tcgccaagtc tgccgaaatt
     2521 cccgggttcc gcaagggcac tgcgcccaaa gaaatggtga aagccaagat tggcgaacaa
     2581 aaaattttgg accgcgccgc ggtgctggcc attgacgatt cgtttcccaa agcggcggcc
     2641 gaaaacaaca ttgagcctct cgggtatccg cagatcagca ttttaaagct ggcgccgggc
     2701 aatccttttg aatataaagc aatcgcggcg gtttatccgc aaacaaaatt gcccgactac
     2761 aaaaggattg ccgcggatct tgaattcaaa gcgccgcaag tttccgccga ggacatcaag
     2821 cgcctccaaa tggagaaaga gcgacatgcc cgccaacatt ggcgcgacga cttgttggaa
     2881 aaactggtca aagagtccga gctggagatt cccgacgttc tggccgcggg agaaacgcaa
     2941 aaagccatgg aagatttcaa agatcgggtg cccaaaatga ccggaatgga ttttgccgaa
     3001 tacttgaaaa aaatcggcaa gaccgaagat caggttcaag aagatatcgc caaagacagc
     3061 gaaacaagaa tcaagaagtt tctaattttg cgggaaatta ttaaagcgga aaaaattgag
     3121 gtcggcgacg aagagattgc cgcggccatt gccaagtcgc gcggcgaaga cggcgaaaac
     3181 gccggggacg ccgaaaacga ggagcaagaa aaagcgtact ggcggcaaag cttgcaatca
     3241 gaaaaagcct ttgaatttct tgaagcaagt tccaaaaaat aattttcaat taacattttt
     3301 cagttttcat tgaattttca attttcaatt gccaattgcc aaacgaacgc aatgcatttg
     3361 tttgtatcat tgaaaattga aacatttatt gaaaactggt cattgaaaat tgaaaattat
     3421 attcaaatga atttaattcc cacggtaatt gaaaaaacgc aatacggcga acgggcctat
     3481 gacatttact cgcgcctgct caaggagcgg atcgtgtttt tgggcgggcc ggtcaacgac
     3541 gttattgcca attccatcat tgcccagttt ctttttctgg cgtcaaaaga cgataaaaaa
     3601 gacattcagc tttatatcaa cagtccgggc ggatcggtga cggcggggtt ggcaatctac
     3661 gacacgatgc aatatatcaa gtgcccggtg tccacggtgt gcgtgggcat ggccgcgtcc
     3721 atgggcgcgg tattgctggc gtcgggggca aaaggcaagc ggtttgccct gcccaatgcc
     3781 gaaattatgc ttcaccaggt ggcgggcggc gcgcaggggg tggcgacgga tattgagatc
     3841 aacgcccggc agatcatccg catcaaagaa tcgctcaaca acatcatcgc caagcacacc
     3901 ggccaaaaga ttgaaaaaat caccgttgac accgaccgcg atttctatct gaccgccgaa
     3961 agcgccaaag aatacggcat cattgacgaa gtgatcaaag ccaagggttg agagcataaa
     4021 gcgcaaag
//
LOCUS       DAOQWE010000070         4013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_22951_length_4013_cov_9.823396, whole genome shotgun sequence.
ACCESSION   DAOQWE010000070 DAOQWE010000000
VERSION     DAOQWE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22951_length_4013_cov_9.823396"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..227
                     /locus_tag="PKK37_02980"
     CDS             18..227
                     /locus_tag="PKK37_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81375.1"
                     /translation="MSNEKSTIGENIKKYRNKLGISQDVLSKKANLAFHTIAKIEAGA
                     TPNPTIDTVKKIADALGVSLDVLMK"
     gene            230..1018
                     /locus_tag="PKK37_02985"
     CDS             230..1018
                     /locus_tag="PKK37_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNR81376.1"
                     /translation="MTKIIKSNSALDEPLEKRELEKAISEKEKKLNDLIEEIEQLKID
                     LTAVKQEYDVKVGRLYLKLDELDLEILKFKKIEDLINKGFSFEEAQKIVEETLKKRRE
                     QIEEEYKKLNEEEKEIETRKGISAEDKEELKKLWRKLAHKYHPDKVGGNEEMMKKINK
                     AYAEGDLETLRAIDQSGTGNGIEALTIEALKNKLAGLELSIKKIHQEYELLKKSEWFI
                     LRENIEKAKKHNRDILSELTDKVLSDIAKKENQVDELKKKYGQR"
     gene            1005..1925
                     /locus_tag="PKK37_02990"
     CDS             1005..1925
                     /locus_tag="PKK37_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81377.1"
                     /translation="MDKDKQIQKIAKYSIDKIAAKSTLATRGLRELGLLKDKKHFTII
                     YTCQFCGRLINYSSTPCAFCCNFPKTKREVIVAQALSSESMEIDSLLAVSKAVKDGED
                     LEIVIANLRDIIDDVLENPSKFSNYPALFKLTEFYVNNPNTVTRAEEINNKIKIFCNH
                     CNNEIILADKPCFYCTVGKNENIENNLMPLQKNIVALNGFLLFAENYLDFSNDKDSMT
                     ELIFVSVYLLNRLLEKDELPDKDLKKYWRELLLRSEYFGSEKTLGAAIEIDANAKPSM
                     VLESAQDKVSSAALNEMIRLIENIIYLLKI"
     gene            1928..2587
                     /locus_tag="PKK37_02995"
     CDS             1928..2587
                     /locus_tag="PKK37_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR81378.1"
                     /translation="MDNNKQIQKVSKLSLEKLSEDSKLVKRGLRDLSIWPKIEELFAK
                     LKAAYENGQIDECMKIAEEILQTDSNHFFTLCYYGRCLYQKGEYEKSLEYLTRCLDEE
                     ENYFFLWKFRGDCYYKMKDYKKAITDYKKSLELDLTNGATLDDIAQCAFLLGDFKTAH
                     DFIDEAIKIESESDMPMIRKAQFFEFQHKNTEAFNQYKRTLEKFPKSEYAQKKVVELS
                     T"
     gene            2592..3602
                     /locus_tag="PKK37_03000"
     CDS             2592..3602
                     /locus_tag="PKK37_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81379.1"
                     /translation="MSISEKLQTSAVATITVLTVPIMLLNLLGGIGSGIWLAILGEWG
                     EIFRGILFMVVAGFAISVALMPSLLFAAPAAMAMERGKKLIGALLGSLSVLYTVALIT
                     IWCIWVMWLFVSSASESSLVPLLVWSYGVALGPWMWLAQKDQQGGGNEFSTLTTFFAQ
                     AAYILGMIMFFFGATLGAIAILFGIIMFAGAILQMVIAFGGEMKKSFSKKEVKEAFEI
                     LEEAGKKFAFASYGFDLVKAHAEKSILSEAKQLTKSVRGGKVTVKKYVYSLITYISGN
                     LISSGQYHIYRGVLNPMGPGEDLLKILDSGLDELVAMGDMSEEEAMEQKEVVRKNIEE
                     MG"
     gene            3611..3931
                     /locus_tag="PKK37_03005"
     CDS             3611..3931
                     /locus_tag="PKK37_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81380.1"
                     /translation="MNKKKNIALIIAATFLFVALFDGWPYGFFTLLRFVVFASSAYVA
                     YMAYEVQKEKWVWIFGFLAILFNPFIVIHLNREIWSVLDLIVGIFMIISVFVLKLEHK
                     PNTN"
BASE COUNT         1414 a          602 c          857 g         1140 t
ORIGIN      
        1 agtaaaacta tacgaatatg tcaaacgaaa aatccacaat cggcgagaat ataaagaaat
       61 accgaaacaa gctcggtatt tcgcaagatg tcttatcaaa aaaggcaaat ttggctttcc
      121 atactatcgc caaaattgaa gcaggggcga cgccaaatcc gactattgat actgtcaaaa
      181 aaattgcgga cgcattgggc gtttcgcttg atgttttaat gaagtaacta tgactaaaat
      241 tataaaatca aattccgcgc ttgacgaacc gctagaaaaa cgcgagcttg aaaaagccat
      301 ttcggaaaaa gagaaaaaac ttaatgattt aattgaggaa attgagcagc tcaaaattga
      361 tttaactgcc gtaaaacaag aatacgacgt gaaagtcggg cgactttatt tgaagttgga
      421 cgagcttgat ttagaaattt tgaaatttaa gaaaattgaa gatttgataa ataagggttt
      481 ttcatttgaa gaagctcaaa aaatagttga ggaaacatta aagaaaagac gagagcaaat
      541 tgaggaagaa tataaaaaat taaatgagga agaaaaagaa attgaaacac gcaaaggtat
      601 ttccgcggaa gataaggaag aattaaaaaa actatggcgc aaactcgcac ataaatatca
      661 ccccgataaa gttggcggca acgaagaaat gatgaaaaaa ataaataaag cgtatgcaga
      721 gggcgattta gaaacactgc gggccattga tcaatctgga accggaaacg gcatagaagc
      781 actgactatt gaagcattaa agaataaact ggcggggctt gaattatcaa tcaaaaaaat
      841 tcatcaagaa tatgaattac tcaaaaaatc agagtggttc atattgaggg aaaacataga
      901 aaaagcgaag aaacacaacc gcgatatttt gagcgagctt actgataaag ttttgtctga
      961 tattgccaaa aaggaaaatc aagttgacga attaaagaaa aaatatggac aaagataaac
     1021 aaattcaaaa aatagcgaaa tattctattg ataaaatcgc cgcaaaatca accttagcaa
     1081 cacgcgggtt gcgagaactt ggactattga aagacaagaa gcattttacc attatttata
     1141 cttgccaatt ttgcggaaga ttaatcaatt attcatcaac gccttgtgct ttttgctgca
     1201 attttccaaa aacaaaaaga gaggtaattg ttgcacaagc cctctcaagc gagagtatgg
     1261 agattgattc gttgttggca gtttcaaagg cagttaagga cggagaagat ttagaaatcg
     1321 tcatagcgaa tttaagggat ataatagacg atgttttgga aaatccttca aaatttagta
     1381 actatccggc attatttaaa ctcacagaat tttatgtgaa taatccgaat actgttacta
     1441 gagcggaaga aataaacaat aaaattaaaa tattttgtaa tcattgcaac aatgagatta
     1501 ttttggcaga taagccatgt ttctattgca cggttggaaa gaatgaaaat atagagaata
     1561 atcttatgcc gttgcaaaaa aatatagttg ccctgaatgg ttttttattg ttcgcagaaa
     1621 attatttaga tttttcaaat gataaagact ccatgacaga attgattttt gtatctgtct
     1681 atctattgaa tcgcctattg gaaaaagatg aattgccaga caaagatttg aaaaagtatt
     1741 ggcgcgaact tttattacga agcgaatact tcggaagtga aaaaacatta ggtgctgcaa
     1801 tcgagataga tgctaacgcc aaaccctcaa tggttttgga atcagcacag gataaagtga
     1861 gcagtgcagc tttaaatgaa atgataagat tgatagaaaa tatcatctat cttttgaaaa
     1921 tttaagtatg gacaataata aacaaattca aaaagtttct aaattatcgc ttgagaagtt
     1981 aagcgaagat tcaaaactcg taaaacgggg tttgagggat ttaagtatat ggcccaaaat
     2041 tgaggaactt ttcgcgaaat taaaagcggc atatgaaaat ggacagatag atgaatgcat
     2101 gaaaattgcg gaggaaatat tgcaaaccga ctcaaaccat tttttcacac tttgttatta
     2161 cggcagatgt ttatatcaaa aaggcgaata tgaaaaatca ttagagtatt taacccgatg
     2221 tcttgatgaa gaagaaaatt attttttctt atggaaattt aggggtgact gttattacaa
     2281 aatgaaagat tataaaaagg caatcacaga ttataaaaaa tctcttgaat tagatcttac
     2341 aaatggcgcg acattggacg atatagctca atgtgcgttt ttgcttggcg acttcaaaac
     2401 cgctcatgat tttattgatg aggcgataaa aattgagagt gaaagtgata tgccgatgat
     2461 aagaaaagca caatttttcg agtttcaaca taaaaataca gaggccttca atcaatacaa
     2521 gaggacactt gaaaaatttc cgaaaagtga gtatgcgcag aaaaaagtag tagaattatc
     2581 aacataaaat tatgtcaatt tcagaaaaat tacaaactag tgcagtcgct acaattacag
     2641 ttctcactgt tccgataatg ttactgaatt tgctcggcgg gattgggtct ggtatttggc
     2701 tggcaatttt aggcgaatgg ggagaaattt ttagaggtat tttatttatg gtagtagctg
     2761 gttttgctat ttctgtagca ttgatgcctt ctttgctttt tgccgcgcct gcggcaatgg
     2821 ctatggaacg cggcaaaaag ctcattggag cattgctcgg ctcgcttagc gtactttata
     2881 ccgttgctct gattacgata tggtgcattt gggtgatgtg gctttttgtc agttctgcca
     2941 gcgaaagctc gcttgttcct ctacttgttt ggtcgtatgg cgttgccctc ggaccgtgga
     3001 tgtggctcgc acaaaaagat cagcaaggtg gaggaaacga attttcaaca ctcacgacat
     3061 tttttgcaca ggcggcatat atcttgggaa tgataatgtt tttctttggc gcaacgctcg
     3121 gagcaatagc aattctcttt ggaataatta tgtttgcggg tgcaattctg caaatggtaa
     3181 ttgcttttgg cggtgaaatg aaaaaatcat ttagtaaaaa agaagttaaa gaagcatttg
     3241 aaatacttga agaagctggc aagaaattcg catttgcaag ttacggattt gatttggtaa
     3301 aagcacacgc tgaaaaatcg attctttctg aagcgaagca acttactaaa tctgtccgag
     3361 gtggaaaagt cacagttaaa aaatatgtct actctttaat cacttatata tctggcaact
     3421 taatttctag tgggcagtat catatctatc gtggcgtgtt aaaccctatg gggcccggcg
     3481 aggatttatt gaaaatttta gattcaggat tagatgaact tgttgcaatg ggcgacatga
     3541 gcgaagaaga agccatggag caaaaagagg ttgttcgcaa aaatattgag gaaatgggct
     3601 aaaaatggag atgaataaaa agaaaaacat tgctttaatt atcgctgcca ccttcttgtt
     3661 cgtggctttg tttgacggtt ggccttacgg attttttaca cttctacgat ttgtagtgtt
     3721 tgcaagttcg gcatatgttg catacatggc atatgaggtt caaaaagaaa aatgggtttg
     3781 gatttttgga tttcttgcga ttcttttcaa tccgtttatt gttatccacc tcaatcgcga
     3841 aatttggagt gttcttgatt tgattgttgg gatatttatg ataatttcgg tattcgtgct
     3901 aaagttagaa cataaaccaa acacgaattg aaatttttag gcggcgcgcc agctaacgct
     3961 ggcacaaatt ccgtcggcgt ccaaagcgag ggcgaggcgg aaggggggtg tgg
//
LOCUS       DAOQWE010000071         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_23334_length_3965_cov_3.619949, whole genome shotgun sequence.
ACCESSION   DAOQWE010000071 DAOQWE010000000
VERSION     DAOQWE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23334_length_3965_cov_3.619949"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3174)
                     /gene="cas12a"
                     /locus_tag="PKK37_03010"
     CDS             complement(<1..3174)
                     /gene="cas12a"
                     /locus_tag="PKK37_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HNR81381.1"
                     /translation="MEKEKTKSIFDNFTRQYSLSKTLRFELKPIGKTRENMNANLEYD
                     ENLRTFLADQKIEDAYQILKPVFDKIHEEFITKSLESAAAKEIDFSEYFNLYKKQKNE
                     QNKDERKKLDKLIQNEEKKLRKEFEKFYLIAGEEFKIIAGKDSNGKDILKENGYKVLT
                     EAGILAYIKNKLLANPAWLDDLKIPEVKVLVENKEKNKEKVEKALEQMKRFFTYFGGF
                     NQNRENYYDVSANKNGEGKSTAVATRVIEENLPKFTDNLLSYEKNKDEYDKVFLKLQN
                     DNKELRLRNPRWDGIDKNTEFIPAYDIKNDGTSFAIGNFVNCFSQSEIDAYNAKVANV
                     NYLVNLYYQQTKNKLPKFKILFKQIGCGEKKDFIKLIKNNAQLKAVLEKAKLAGEKYL
                     INDKDKDAVGAFMDYLLNLDDFKNIYWSDKAMNTISGKYFGNWGNLKDKLLKAKVFNK
                     DKNSGEAKIPQAVSLSDLFEVLDAEKDWQGKGILFRENFKENNEEKQDIIKNSRTPHD
                     ALLKMICKDIENLAQKFVDGADEILKIKEGDYQKDESKIAIKTWLDSALFVGQILKYW
                     RVKARFSVDSNFTDILDKVKVSETVQDYDVVRNYLTQKPQNKLGRLKLNFENSTLAGG
                     WDVNKEKDNACLILQDKKGKQYLVVTECSGAGIFEQTAQNSLYAPDASGWTKMNYKLI
                     PGPNKMLPKVLFFSSKWIKANPTPDNIKTIYEKGTFKKGDNFSQNDLHALIDFYKDQL
                     KKYPLPEESWNRIFDFDFSDTKKYESIDQFYTEVERQGYKIEFVPINKGKIDELVEGG
                     RIYLFEIKSKDSNLKNGKEKTSAKDLQTIYWNEIFGDAENKPKLNGGAEIFYRSALEE
                     ADLKKKNWKNKEIIENFRFSKEKFILHCPITLNPCLKNNRINDLVNKNFAKSKNQLFL
                     GIDRGEKHLAYYSLVNRKGQILEQGSFNTINGQDYWDKLDKKQGDRDEARKNWTTIGN
                     IKDLKDGYISQVVRKIVDLAVYNEGDRKKGFRETPAFIVLEDLNIGFKRGRQKIEKQV
                     YQKLELALAKKLNFLVDGRMVKGDIRER"
     gene            complement(3179..3469)
                     /locus_tag="PKK37_03015"
     CDS             complement(3179..3469)
                     /locus_tag="PKK37_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013671666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81382.1"
                     /translation="MEKEVILKLHKNFNDCVHFENGIEFWYARELQLLLGYDKWSNFE
                     KVIDKARIACDVAGEVIHNHFADVGKMVDKRKIRSETKKLPKESKKLTNNKK"
     gene            complement(3782..>3965)
                     /locus_tag="PKK37_03020"
     CDS             complement(3782..>3965)
                     /locus_tag="PKK37_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81383.1"
                     /translation="AAQSGDLAVGQSVTAAGTAGTDGSIVANNIQIGAPARPAGTPPA
                     APADENQEEPAVQPVM"
BASE COUNT         1055 a          838 c          604 g         1468 t
ORIGIN      
        1 cctttcgcga atatcgcctt tgaccatccg gccatcaaca agaaaattca acttcttggc
       61 caacgccaat tcaagttttt gataaacttg tttttcaatt ttttgccgac cgcgcttaaa
      121 gccgatgttt agatcttcca acacaataaa tgcgggcgtt tcgcgaaaac cttttttcct
      181 atccccctcg ttataaaccg ccaaatcaac aatttttctc acgacttgcg agatatatcc
      241 gtcttttaag tcttttatat tgccgattgt cgtccaattt ttgcgcgcct catcacgatc
      301 accttgtttt ttatccaatt tatcccaata atcttgacca tttatcgtat taaaacttcc
      361 ctgctctaaa atttgacctt ttcgattcac gagagagtaa tatgcaagat gtttttcgcc
      421 gcggtcaatc ccaagaaaca attgattttt gctttttgca aaattcttat ttaccaaatc
      481 attgattctg ttatttttta agcaaggatt taaagtgatc ggacagtgta aaataaattt
      541 ttctttgctg aagcgaaaat tctcaataat ttccttgttt ttccaatttt tcttttttaa
      601 atccgcttct tccagcgcgg accgataaaa aatttccgcg ccgccgttta gtttcggctt
      661 attttcagca tctccaaaaa tctcattcca ataaattgtt tgcagatctt ttgccgatgt
      721 tttttcttta ccgtttttca aattactatc tttgctttta atttcaaaaa gataaattct
      781 cccaccctca accaattcgt caatcttccc tttgttgatt ggcacaaatt caattttata
      841 gccttgtctt tcaacttcgg tataaaactg atcaatgctt tcatattttt ttgtgtcaga
      901 aaaatcaaaa tcaaaaatcc gattccaact ttcctccggc aagggatatt tttttaattg
      961 atctttataa aaatcaatca gcgcgtgcaa atcattctgg ctgaaattat ctcccttctt
     1021 aaacgttcct ttttcgtaaa ttgttttaat gttgtcggga gttggattgg ctttaatcca
     1081 tttcgatgaa aaaaataaaa ctttgggcaa catcttattc ggaccgggta tgagcttata
     1141 attcattttt gtccatcctg aagcgtcagg tgcataaagt gaattctggg cggtttgctc
     1201 aaaaatacca gcaccactgc attccgttac cacaagatac tgctttcctt tcttatcctg
     1261 caaaatcaaa caggcgttat ctttttcttt gttcacatcc cacccgccag ccagagtgga
     1321 attttcaaaa ttcaatttta atcttccaag tttgttctgc ggcttttgag ttaaatagtt
     1381 tctaacaaca tcataatctt ggacagtttc cgaaacttta actttgtcaa gaatatcagt
     1441 aaaattactg tcaacggaaa acctcgcttt caccctccag tattttaaaa tttgtccgac
     1501 aaaaagcgca ctgtcaagcc aagttttaat tgcgatcttg ctttcatctt tttgataatc
     1561 cccttcttta atcttcaaaa tttcatccgc cccgtcaaca aatttttgcg ccaaattttc
     1621 aatatctttg caaatcattt tcaaaagcgc atcgtgcgga gttcgggaat ttttgataat
     1681 atcctgtttt tcttcattat tttccttaaa attttccctg aataaaatgc ctttcccttg
     1741 ccaatccttt tccgcatcca aaacttcaaa caaatcggaa agcgaaacag cttgcgggat
     1801 ttttgcctcg ccgctgtttt tgtctttatt gaatactttt gctttcagta atttgtcttt
     1861 cagatttccc caattcccaa aatattttcc ggaaatggta ttcattgctt tgtccgacca
     1921 ataaatattt ttaaaatcat ccaaattcaa aagatagtcc atgaaagcgc ccacggcatc
     1981 tttatcctta tcatttataa gatatttttc gccggccaat ttcgcttttt caagcacagc
     2041 ttttaattgt gcgttatttt ttatcaattt tatgaaatct ttcttctcgc cacagccaat
     2101 ttgcttgaat aaaattttga atttgggaag tttatttttg gtctgttgat aatacaaatt
     2161 gaccagataa ttcacattgg caacttttgc attataggca tcaatttctg attgcgagaa
     2221 gcagttcaca aaattaccaa tcgcaaaact tgttccgtca tttttaatat cataagcagg
     2281 aataaattcg gtgtttttat ctattccatc ccatcttggg tttcttaatc ttaattcttt
     2341 attgtcgttt tgaagtttca aaaaaacttt atcatattca tctttatttt tctcgtaaga
     2401 aagtaagttg tcggtaaatt tcggcaaatt ttcttctatc actctggtcg caactgccgt
     2461 tgatttacct tcgccgtttt tatttgcgga aacatcataa taattctcgc ggttttggtt
     2521 aaatccgccg aaataggtga aaaatctctt catttgctct aacgcctttt caactttttc
     2581 cttatttttt tctttatttt caactaaaac tttcacttcc ggtattttta aatcatcaag
     2641 ccaagcagga ttagctaaaa gtttattttt gatatatgca agtatcccgg cttctgtcaa
     2701 aactttatag ccattttctt ttaaaatatc cttgccatta gagtctttgc cggcaataat
     2761 tttaaattct tctcccgcaa tcaaataaaa tttttcgaat tcttttcgta gcttcttttc
     2821 ctcgttttgg atcagtttat ctaatttttt tcgctcgtct ttgttctgtt catttttttg
     2881 ttttttatac aaattaaaat attcggaaaa atcaatctct tttgccgcgg cgctttcaag
     2941 ggatttggtg ataaattcct cgtggatttt gtcaaaaacc ggctttaaga tttgataagc
     3001 gtcttctatt ttttggtctg ctaaaaaagt ccgtaaattt tcatcatatt ccagatttgc
     3061 attcatattt tcccgtgttt ttccaatcgg ctttaactca aaccgcaagg tttttgacag
     3121 cgaatactga cgagtgaaat tgtcaaaaat tgattttgtt ttttcttttt ccatattttt
     3181 attttttatt attggttaat tttttagatt ctttgggaag ttttttggtt tcggatctta
     3241 tttttctttt atcaaccatc ttgccaacgt cggcaaaatg gttgtggata acttcgccgg
     3301 caacatcaca agcaattctc gccttgtcaa tcactttttc aaaattactc cacttatcat
     3361 agccaagcaa taattgcagt tctcgcgcat accaaaactc aatcccattt tcaaaatgca
     3421 cgcaatcatt aaaattttta tggagtttaa gaatgacttc tttttccata gtttattttc
     3481 ttgaattaca aaactcttgc tacaagtgat ttgtaatcca aagttattta tttttgcaaa
     3541 aagggacgac ggttgtcgcc caatagtaac gcttaccaaa cggttaagca aattggaacc
     3601 gtcgcccctt tttatttaaa accaagcttt gcggttttat aaaaaataag gccaaccgtt
     3661 attgataaac gatactattg tgcgactggt ttattatcgc acaagccgca gaaaagtcaa
     3721 attttttgac acaaaagcgc ggtacaaatg ccgcgctttg gggaggtttt tggctgaatt
     3781 tttacattac gggctggacg gcgggttcct cctgattttc atcggccggg gcggccggcg
     3841 gggttccggc aggacgcgcc ggcgcgccga tttggatgtt gttggcgaca atactgccat
     3901 ccgtgccggc ggttcccgcc gccgtcaccg actggccaac cgccagatcg ccggattgcg
     3961 ccgcg
//
LOCUS       DAOQWE010000072         3887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_24004_length_3887_cov_7.321242, whole genome shotgun sequence.
ACCESSION   DAOQWE010000072 DAOQWE010000000
VERSION     DAOQWE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24004_length_3887_cov_7.321242"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PKK37_03025"
     CDS             complement(<1..849)
                     /locus_tag="PKK37_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNR81384.1"
                     /translation="MFDVITFGSAAWDVFMDLPPKFIKKSAHFTPAKDMVFDLGSKID
                     VPGTRFAFGGGGINAARTFAAQGLSTAYCGMAGDDIPGREAVAYLNELGIDTGFLSKT
                     AKKATNNSVVLNAPGTDRVILVYRGASELLAAENIDWQKLNAKLFYLAPLSGKLCRLT
                     ERITLHAKKRGAMVAANLGNSQLAMGRACIKSLLARIDVLILNREEAAMMTGVDYADE
                     AGIIKKIVHMHSGISVITKGEDGAAVVAGGKIYEAKLKKFKIANITGAGDAFGSGFMS
                     GMLASGN"
     gene            complement(896..1177)
                     /locus_tag="PKK37_03030"
     CDS             complement(896..1177)
                     /locus_tag="PKK37_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81385.1"
                     /translation="MGYEPIYSIKFKKNVRDLAGKDGKIKDGLTKAIPFILEKPYYKS
                     EKIKGEDGNLMRKHICANNFRIFYYVDEKNKKVTFAMLRPKNKNTYKNL"
     gene            complement(1192..1398)
                     /locus_tag="PKK37_03035"
     CDS             complement(1192..1398)
                     /locus_tag="PKK37_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81386.1"
                     /translation="METVFQTGCKNTRHLSKMTNRRDFNYYWKEIISGELSFEDKFEL
                     EQAKDDIRNKRFATHEEIMDILRK"
     gene            complement(1414..1647)
                     /locus_tag="PKK37_03040"
     CDS             complement(1414..1647)
                     /locus_tag="PKK37_03040"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNR81387.1"
                     /translation="MEEAKKEVKINIPDNLKNGVYSNIANINVSKSEVLMDFIFLAPN
                     EANLVSRIIVSLEHARSIEKALSKLIAEIDNKK"
     gene            complement(1713..>3887)
                     /gene="ppsA"
                     /locus_tag="PKK37_03045"
     CDS             complement(1713..>3887)
                     /gene="ppsA"
                     /locus_tag="PKK37_03045"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNR81388.1"
                     /translation="DIFNGLDVNNLKQLKKASITARNLVLKGEFPPDFKNEILAAYKE
                     LSAYYKTKNLNVAVRSSATAEDLAGASFAGAHETYLNIDTPAELLDAVKKCISSLYLD
                     RAIVYRAEKGFDTLKVALSVGVMKMVRSDMASAGIMFTLDTETGFRNAVLINSIYGAG
                     EMIVKGHVVADQFYVFKPSLKSGKSAIISKTLGNKDIKYIFDKKVGLKEVKVLPKDQA
                     RFSLTDKEAEQLAKWAVIIEEHYQGPQDIEWAKDGKTGQLFIVQARPETIYAGTEIRN
                     TYEEYEVKTSAKPLVTGIAVGNKIGEGKIHIIPNVKQIGQFKKGEILVTTMTDPDWVP
                     IMRIASAIVTDEGTKTCHAAIVSRELGTPCIVGARNATKVLKNGIIATADCTQGLEGR
                     VYAGKIPYTKKEYDLGTIPELPTKIMLNVGTPEGAFKASFLPNDGVGLARVEFIFADK
                     VKVHPLALINFARLKDKKVKAQIEKITTGYKDKKEFFVDKLAEGVATIAAAFHPKHVI
                     VRFSDFKTNEYRELIGGKDYEPLESNPMIGWRGASRYYDPRFAPAFAMECAAIKKVRE
                     TMGLDNVWVMVPFCRTVEEGKKVIEAMKNNGLQSGVNGLKIMVMCEIPSNVILADRFL
                     EIFDGMSIGSNDLTQLALGLDRDAGQLSYIANENNEAVKMLVAQAIKACKARGKYIGI
                     CGQAPSDYPEFAKFIMREGIETMSLNPDSVMKTIIKLGGLQK"
BASE COUNT          926 a         1020 c          789 g         1152 t
ORIGIN      
        1 gttgcccgac gccagcattc ccgacataaa ccccgaaccg aaagcgtctc ccgcgccggt
       61 gatgttggca attttgaatt tcttcaattt cgcttcgtag attttgccgc cggcaaccac
      121 cgccgcgccg tcttcgcctt tggtaatcac gctgatcccc gaatgcatat gaacgatttt
      181 tttgatgatg ccggcttcat cggcataatc aacgccggtc atcatcgccg cctcttcccg
      241 gttgagaatc aagacgtcaa tgcgcgccag caggcttttg atgcatgcgc gccccatcgc
      301 cagctgggaa ttgcccaaat ttgccgccac catcgcgccc cgctttttgg catggagggt
      361 gatgcgctcc gtcaggcggc acagcttccc cgatagcggc gccagataga acaatttggc
      421 attcagcttt tgccaatcaa tgttttccgc cgccaaaagc tcggatgcgc cgcgatagac
      481 caaaatcacc cggtccgttc cgggagcgtt caagaccacg gaattattgg tggccttttt
      541 cgccgttttg gaaagaaatc cggtatcaat ccccaattcg ttcaaatagg cgaccgcttc
      601 gcgtccgggg atatcatcgc cggccatgcc gcaataggcc gtggaaagac cctgcgccgc
      661 aaacgtccgg gcggcgttga ttccgccgcc gccgaaagca aaccgcgtgc cgggaacgtc
      721 aatttttgat cccagatcaa aaaccatgtc cttggccgga gtgaagtgtg cgcttttctt
      781 gatgaatttc ggcggcagat ccatgaacac atcccatgcc gcgctcccga aagtgatcac
      841 atcaaacata aatttggata atttccgatg tcaagcaaaa attaaaatca aaaaattaca
      901 gatttttgta agtattttta tttttcggcc ttaacatggc gaaagtaact tttttatttt
      961 tttcatcaac ataataaaaa atccggaagt tattggcgca gatatgcttg cgcatcaaat
     1021 tgccatcctc gccttttatt ttttctgatt tataataagg cttttccaaa ataaacggga
     1081 tggcttttgt caaaccatcc tttatcttgc catccttccc cgccaaatct ctaacatttt
     1141 tcttaaattt aattgaatat atcggctcgt agcccacaat ttatttaaat attattttct
     1201 tagaatgtcc ataatctctt catgcgttgc aaatctttta ttcctaatat catctttggc
     1261 ttgttctaat tcaaatttat cctcaaatga tagctcaccg cttattatct ctttccaata
     1321 ataattaaag tcgcgacgat ttgtcatttt acttaaatgt cttgtgtttt tacaaccggt
     1381 ttgaaacaca gtttccataa atttatagtg ttattatttt ttattgtcaa tttcagcaat
     1441 aagctttgat aatgcttttt ctatggatct ggcatgctcc aacgaaacga taatccggct
     1501 aactaaattt gcttcattgg gtgcgagaaa aataaaatcc atcaacactt cgctcttaga
     1561 gacattgata ttggcaatat ttgagtaaac tccgtttttc aaattatcgg gaatattaat
     1621 tttaacttct ttttttgctt cttccatatt ttttaatgat actttttatc tatcacataa
     1681 aatagtttaa ggcaataata gaaaatcaca catcacttct gcaaaccgcc taatttgatg
     1741 atggttttca tcacactgtc gggattcaaa gacatggttt caatgccttc gcgcataata
     1801 aatttggcaa attccgggta gtcgctgggg gcctggccgc agatgccgat atattttccg
     1861 cgcgccttgc aggccttaat cgcctgtgcc accagcattt ttaccgcctc gttgttctcg
     1921 ttggcgatat acgataactg tcccgcatcc cggtccaatc ccagcgccaa ttgcgtcaaa
     1981 tcgttggagc cgattgacat cccgtcaaaa atctccagga atctgtcggc aagaataaca
     2041 tttgacggaa tctcgcacat caccattatt tttaatccgt tgacacccga ttgaagaccg
     2101 ttatttttca tggcttcaat caccttttta ccttcctcga ccgtgcggca aaacggtacc
     2161 atcacccaaa cattgtccaa ccccatcgtt tcgcggactt ttttaatcgc cgcgcattcc
     2221 atcgcaaacg ccggggcaaa ccgcggatcg taatatcttg acgcgccgcg ccagccgatc
     2281 atcgggtttg attccaatgg ttcatagtcc ttgccgccga tcaactcgcg gtattcgttg
     2341 gttttgaaat ccgaaaagcg cacgatcaca tgcttgggat gaaacgccgc cgcgatcgtg
     2401 gccacccctt cggccagctt atctacaaaa aattcttttt tatctttata gccggtggta
     2461 atcttttcaa tctgcgcctt tacttttttg tccttcaacc gggcaaaatt aatcagcgcc
     2521 agcgggtgca ccttgacttt gtcggcaaaa ataaattcca cccgcgccag ccccacgccg
     2581 tcattaggca gaaaagacgc tttgaacgcg ccttccggcg tgcccacatt cagcataatt
     2641 tttgtcggca attccggaat tgtccccaga tcatattctt tcttggtata gggaattttt
     2701 cccgcgtata cccggccttc caaaccttgg gtgcaatcgg cggtggcaat gattccgttc
     2761 ttcaaaacct tggtggcgtt tctcgcgccc acgatgcagg gcgtgcccaa ttcgcgcgaa
     2821 acaatggccg cgtggcaggt tttggtgcct tcatcggtca cgatcgccga cgcgatgcgc
     2881 ataatcggca cccaatccgg atcggtcatt gtcgtcacca gaatctcgcc tttcttgaat
     2941 tggccgattt gtttgacatt gggaatgata tggatttttc cctctccgat cttgtttccc
     3001 acggcaatgc cggtcaccag cggcttggcg ctggttttca cctcgtattc ttcataggta
     3061 ttcctgattt ccgttccggc atagatggtt tccggccgtg cctgaacgat aaataactgg
     3121 ccggtcttgc cgtctttggc ccattcaata tcttgcggcc cttggtagtg ttcctcaatg
     3181 atcaccgccc atttggccag ctgttcggct tctttatcgg tgagtgaaaa tctggcttgg
     3241 tctttgggaa gaactttcac ttcttttaag cccacttttt tgtcaaaaat atattttatg
     3301 tctttattgc ccaaagtttt ggaaattatc gcggattttc cggacttcag cgacggcttg
     3361 aaaacataga attggtcggc taccacatgg cccttcacaa tcatttcacc cgcgccatag
     3421 attgaattta tcaacaccgc gttgcggaat ccggtttccg tgtccaaggt gaacataatt
     3481 cccgcggagg ccatatccga gcgcaccatc ttcatcacgc ccaccgacag cgccacttta
     3541 agcgtgtcaa atcctttttc cgcgcgataa acgattgccc gatccaaata cagcgacgaa
     3601 atgcattttt tcaccgcatc cagcagttcc gccggcgtgt caatattcaa ataggtttcg
     3661 tgggcgccgg caaacgatgc tccggccaag tcttccgcgg tcgcggatga ccgcaccgcc
     3721 acattcaaat ttttggtttt ataatatgcc gacaattctt tgtaggccgc cagaatttca
     3781 ttcttaaaat ccggcggaaa ttcgcccttg agaaccaaat tccgcgcggt aatgctggct
     3841 tttttgagtt gttttaaatt attgacatcc aagccattga atatatc
//
LOCUS       DAOQWE010000073         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_24502_length_3834_cov_7.215136, whole genome shotgun sequence.
ACCESSION   DAOQWE010000073 DAOQWE010000000
VERSION     DAOQWE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24502_length_3834_cov_7.215136"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PKK37_03050"
     CDS             <1..524
                     /locus_tag="PKK37_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNR81389.1"
                     /translation="KELAKIWQLPVKNNSESQDVCFISGQPADFFKKHLGGNPGDIVD
                     ADAKKIIGRHRGLWFYTVGQRKGIGLPGGPFYVIGKNLAKNRLLVSKKMPAASSVKLS
                     NINWIAGAVPDLPRKINIKIRYRAKEAAAVLDKIGKDYVLRFAKPQFAPTPGQSAVFY
                     VRGELIGGGIIKP"
     gene            625..2256
                     /locus_tag="PKK37_03055"
     CDS             625..2256
                     /locus_tag="PKK37_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNR81390.1"
                     /translation="MKNVAKKKAALFKTKIKNKSKRPVPLPSGKAKPLIKIAEKIRGK
                     VLNIKAVETAKIAPPPAKKTVKNAAGESEFEIKKTKIRVVGIGGGGGNIVSEIADRVR
                     RPSFTIANTDAAALRRASKNVSVFQFGENFTRGLGTGMDPRVAEEAAVQEKEKIKKIM
                     GGQDLVIIVASLGGGAGSGAAPVFAQISKNLGNLTYGIFTLPFEFEGEKKKEIAKAAL
                     EKSKPYLNALTILPNERVFEVVPKATPFSKTLSFINDSLSDSLEGLIETIYSTGLINI
                     DFADLRAILVGSGKMAFLNTVALKKGEETGVDVFEKVFSSPLYPYGIEKSRGLLLNIV
                     AQKDLKLAEVNKILTGAAKRINKDARIIFGVGHEGARESSIKVTILATGCVYAGAGNG
                     NGDDGNGKEVPVAPPVPRVPRKAAPKKTAALPPKPAKPVKSAKPVKPAKPVKSATALR
                     KSKSAPVKLKKVAVKIKSLPPAAKEPQSAPEKQIQDGAGRQSGQGIDAQFTAPERKNA
                     LQVKKEIEMEENAMLEQEKVWEVPAFLRKQKKNQN"
     gene            2309..3250
                     /locus_tag="PKK37_03060"
     CDS             2309..3250
                     /locus_tag="PKK37_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR81391.1"
                     /translation="MYDLIIIGAGPAGMTAGIYAARAKLETLVIAGEFGGWIARENVE
                     IENYPGFEKVPGKELIDRFKSHLESFGVRTKMAEVFELKKQGGIFEVSAGEEKFSAKA
                     LIVATGASPRPAGIPGEKDFLGKGVCYCATCDGPLFAKKDVAVIGGGNAAFDAARFLA
                     KFAAKVYVLEKNKEVSADAASREALERTGKARIITNARITKISGDNFVKGVAYYDAAD
                     PEKREIALPVAGVFVKAGNSPAAEFLGNLVDLDEKGQIKINSQNNQTKTEGLFAAGDV
                     TDAKYKQVVIAAGQGAVAALSAGLYLQNSKFQGSNSK"
     gene            3348..>3834
                     /locus_tag="PKK37_03065"
     CDS             3348..>3834
                     /locus_tag="PKK37_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81392.1"
                     /translation="MKIDFKAKKRDLGQFFTTNADYILQGFEKFVEGKTISDPFAGNR
                     ELLNWCQRNGAKTAIGFDCDKNYVDDKDVFFNDSINLPKKYDFVCTNPPYLHKNKASV
                     SVKNKFFTGENSIFEDLYQVSIKALLNCNEGITIVPLNFLCAQNSQRIRNLFFEKFEI
                     VK"
BASE COUNT         1100 a          799 c         1045 g          890 t
ORIGIN      
        1 tcaaagaatt ggcaaaaatc tggcaattgc cggtaaaaaa caattcggag tcgcaggacg
       61 tttgcttcat ttccggccaa cccgcggatt tttttaaaaa acatttgggc ggaaatccgg
      121 gggatattgt tgatgcggac gcaaaaaaaa ttatcgggcg gcatcgcggc ctgtggtttt
      181 acaccgttgg ccagaggaaa ggcatcgggc ttccgggcgg cccgttttat gtcattggta
      241 aaaatcttgc caaaaaccgg ttgctggttt caaaaaaaat gccggcggcg agttcggtta
      301 aactgtcaaa tatcaattgg attgccggcg cggttcccga tttaccgcga aaaatcaaca
      361 taaaaatcag ataccgggcc aaagaagccg cggcggtttt ggataaaatc ggcaaagatt
      421 atgtgttgcg gttcgcaaaa ccgcaatttg cgccgactcc cgggcagagc gcggtttttt
      481 acgtccgcgg cgagttgatc ggcggcggta ttatcaagcc gtgatccttt gttgcggctg
      541 tgtttctttt gcgtttgggg cggttttcgt gctaatctgg aagaataatt gagtataaca
      601 aaggaaaatc ctttttaaac cggtatgaaa aatgttgcca aaaagaaagc cgctcttttt
      661 aaaaccaaaa tcaaaaataa atccaaaaga ccagtaccgt tgccgtccgg gaaagcaaaa
      721 ccgttgataa aaattgccga aaaaatccgc ggaaaggtgc tcaatatcaa ggcggtggaa
      781 acggctaaaa tcgccccgcc tccggccaaa aaaacggtca aaaacgcggc tggagaatcg
      841 gaatttgaga tcaagaaaac caaaatccgc gtggtgggga ttggcggcgg cggcggaaat
      901 atcgtttcgg aaatcgccga ccgggtgagg cggccttcct ttactattgc caacaccgat
      961 gcggcggcgc tgcgccgcgc gtcaaagaat gtgtcggtgt ttcagttcgg agagaatttt
     1021 acgcgcgggc tgggtacggg gatggatccg cgcgtggccg aagaggccgc cgtgcaggaa
     1081 aaagagaaga tcaagaaaat tatggggggg caggatctgg tgattatcgt ggcttcttta
     1141 ggcggtggcg ccggatcggg cgccgcgccg gtgttcgcgc aaatttcaaa aaacttgggc
     1201 aacctgacct atggaatttt tactttgccg tttgaatttg aaggcgagaa aaagaaggag
     1261 atcgccaagg cggcgctgga aaaatccaaa ccctatttga acgccttgac cattttgccc
     1321 aacgagcggg tgtttgaagt ggtgcccaaa gcaacgccat tttccaagac gctttcattt
     1381 atcaacgatt cgctttccga cagtttggag ggattgattg aaaccattta cagcaccggg
     1441 ttgatcaata ttgattttgc ggatctgcgg gcgattctgg tggggagcgg gaaaatggcg
     1501 tttttaaaca cggtggcgct gaaaaagggc gaagaaacgg gcgtggatgt ttttgaaaag
     1561 gttttttctt ctccgctgta cccttatggg attgaaaaat cgcgcgggtt attgctgaat
     1621 atcgtggcgc aaaaagattt gaaacttgcc gaagtgaaca agattctcac cggtgccgcc
     1681 aaaaggatta ataaggatgc tcggattatt ttcggcgttg gccatgaagg tgcgcgcgaa
     1741 agttcaatca aagtgacgat tttggccacc ggatgcgttt acgccggcgc cggaaacgga
     1801 aacggcgatg atggtaatgg caaggaggtt ccggttgcgc cgccggtacc gcgggtgccg
     1861 agaaaagcgg cgccgaaaaa aacggcggct ttgccgccaa aaccggccaa gcccgtgaaa
     1921 tctgccaaac cggtaaaacc ggccaagccc gtaaaatccg ccacggcttt acggaagagc
     1981 aaatccgcac cggtaaaatt gaaaaaagtg gcggtgaaaa tcaagtcgtt gccgcccgcc
     2041 gcaaaggagc cgcaatccgc accggaaaaa caaattcaag acggggccgg acggcagagc
     2101 gggcaaggga ttgacgctca atttaccgca ccggaaagga aaaacgctct gcaggtgaaa
     2161 aaagagattg agatggagga aaatgcgatg ctggaacagg aaaaagtttg ggaggtgccg
     2221 gcgttcttgc gaaaacaaaa gaaaaatcaa aattgaaaaa tcaaaatgta aaattaaggg
     2281 aaaattcctt gcggaatttt atatttttat gtacgactta atcattattg gtgccggacc
     2341 ggccggaatg actgccggaa tctacgcggc gcgcgccaag ttggaaacgc tggtgatcgc
     2401 cggcgagttc ggcgggtgga ttgcgcgcga aaacgtggag attgaaaatt atcccggatt
     2461 tgaaaaagtt ccgggcaaag aactgattga ccgcttcaaa agccatctgg aatcttttgg
     2521 cgtccgaaca aagatggccg aagtttttga gttaaaaaag caaggcggca tttttgaagt
     2581 ttcggccggc gaagaaaagt tttcggccaa agcgctgatt gttgccaccg gcgccagccc
     2641 gcggccggcg ggcattccgg gagaaaaaga ttttttgggc aagggtgttt gctattgcgc
     2701 aacatgcgac ggaccgctgt ttgccaaaaa agacgtggcg gtgatcggcg gcggcaacgc
     2761 ggcgtttgac gcggcgcggt tcttggcaaa attcgccgcc aaagtttatg ttctggaaaa
     2821 aaacaaagaa gtttcggccg atgcggccag ccgggaggcg ttggagcgaa ccggcaaagc
     2881 ccggataatc accaatgcgc ggatcacgaa gattagcggc gacaatttcg taaagggagt
     2941 tgcctactat gatgccgccg atccggaaaa aagggagatt gctttgccgg tggccggagt
     3001 ttttgtgaaa gccggcaatt cgccggccgc ggaatttttg ggcaatttgg ttgatttgga
     3061 tgaaaagggc cagataaaaa tcaattccca aaataaccaa accaaaaccg aaggtttgtt
     3121 tgccgccggc gacgtaaccg atgccaaata caagcaagtg gtgatcgccg ccggccaggg
     3181 cgctgttgct gcgctctccg ccggtttgta tctccaaaac tccaaattcc agggttcaaa
     3241 ttccaaataa atacgatttg aatacgattt aaatacgatt cagatacaat ttaaatcccg
     3301 gcaatcgcgg gattttgatt tcttcctgct tgcggtataa tagtaatatg aaaattgatt
     3361 ttaaggcaaa aaaacgggat ttggggcaat ttttcacgac caatgccgat tatattctgc
     3421 aaggatttga gaaatttgtt gaaggcaaaa caattagcga tccgttcgcc ggaaatcggg
     3481 aattgcttaa ttggtgccaa agaaacggcg caaaaaccgc cataggtttt gattgcgata
     3541 aaaattatgt cgatgataaa gatgtctttt ttaacgattc gataaatttg cccaaaaaat
     3601 acgattttgt ttgcaccaat ccgccctatc tgcataaaaa taaggcttcg gtttcggtta
     3661 aaaacaaatt ttttaccggt gaaaattcaa tttttgaaga tttgtatcaa gtatcaatta
     3721 aggcattgct gaattgcaac gaaggaataa ctattgtgcc attgaatttt ttgtgcgcgc
     3781 aaaattcgca acgaataaga aatttatttt tcgaaaaatt tgaaattgtt aaat
//
LOCUS       DAOQWE010000074         3811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_24743_length_3811_cov_6.960330, whole genome shotgun sequence.
ACCESSION   DAOQWE010000074 DAOQWE010000000
VERSION     DAOQWE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24743_length_3811_cov_6.960330"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="pilM"
                     /locus_tag="PKK37_03070"
     CDS             complement(<1..490)
                     /gene="pilM"
                     /locus_tag="PKK37_03070"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNR81393.1"
                     /translation="MLDFLSLSKEIYGLEIADTYARMMRLAHRNGGLVVVAAGFLRLP
                     EGAVQSGEIRDEKSLAAAISELAGKTGAAGTGRAAVVSLPEDKAFWQIIKMPRLDDDE
                     LRGAVIFEAENYIPLPLDKVYLDFEVVPSSLIENRCEVAVAALPKEIVDSRVRVCDAA
                     KLR"
     gene            complement(605..>1806)
                     /locus_tag="PKK37_03075"
     CDS             complement(605..>1806)
                     /locus_tag="PKK37_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81394.1"
                     /translation="SSGWAKDYPAVKRTVKAVYGRESVAKYSYVLNANVWIGDDHQIN
                     GPYHSNGGIRMDGGNQSLMTSAAIIDDEGKWVCTESFGCCQWRYFWYPYYKNGYDCAL
                     DCPSACTVNVTGTKLVDGRPVKDECVCPGIFSTTANPSPDLFKFPVPPFDFNGVTVDL
                     GQIKERAVNGGGIYFPPAADRKPGSKGYHVKLREDGKFEVYFIMALQQVSGYSEEEGD
                     VKDWFIINNEQADANNPYTIPSDCAVLYFEDNLWIEGTVRGKITVASANLISTGVETS
                     VVLPGNIQYVDYSGANGLALIGQKNVLIGPQSPEDMVLDAVIVAQKGRFGRNHYEDNF
                     RDSLTIRGSVISNGRVGTQWTSGGQMVSGYAQRYTYSDQGQVYNPPPFVMSMGSDYKI
                     VSWEEME"
     assembly_gap    1807..1906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1907..2511)
                     /locus_tag="PKK37_03080"
     CDS             complement(<1907..2511)
                     /locus_tag="PKK37_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR81395.1"
                     /translation="MNKTKKNLLSDRQNIFGCCPGRGFTIIEALTAVAVFSLLMAAAG
                     GLVAIVYKIHDFAFEQTIAADEARRGADVMIKEIRQARYGDNGAYPIEKAADKELIFY
                     ADIDGDGTTERVRYFLGTVNSGQITKECHSFSQGGSCSADIGGFLSGNLKFAQVKVYT
                     EGYYGTSSRYAEFFIDGIKIGNLCQGSCLQCAAAWQGAETY"
     gene            complement(2504..>3811)
                     /locus_tag="PKK37_03085"
     CDS             complement(2504..>3811)
                     /locus_tag="PKK37_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNR81396.1"
                     /translation="SKVQEAQSCQSQPGGVLTVTVFDDGGSLVPSPEIKIVDLSTGIQ
                     TASATPETGRHSFPLSPQSYRVEVSKTDYSAARTYGSDEVAVPESPHPTVLQDSETPM
                     SLSIDRTASISVEAIAPVGQGNFSDNFDDESLLAETSNVAIAAGNATLVGPPYSSGYA
                     VSQSIAPGDLAAWNEIQFSDQRPPGTEIRYQLLYYDGVDWMLIPDADLGQNSAGLTVS
                     PINLMGLDKGIYPAIRIRAQLSTSDPEATPEILNWQVFWSNSAGTPVPDAVIHLQGSK
                     IIGEDENGEKVYKYSQEHNLGGAGRADISGIDGDSYYFSVVPGQGLALIGSDPPTMPV
                     QAPSGGAAAVKLYLQAPNGLLISVFDAVTLAPVFSATVKVKNETLGYEKTQITDSGGE
                     TYFTFLQDGEYEISVSALGYQEFIQSKTIAGDTSEAISIVQNE"
BASE COUNT          914 a         1080 c          843 g          874 t
ORIGIN      
        1 gccgcaactt cgcggcatcg caaacgcgga cgcgggaatc cacaatttct ttgggcaaag
       61 ccgccaccgc aacctcgcaa cggttttcaa tcagcgacga aggcaccacc tcaaaatcaa
      121 gataaacttt atccagcggc aggggaatgt agttctccgc ctcaaaaatc acggcaccgc
      181 gcaattcatc gtcatcaagg cgcggcattt tgattatttg ccaaaacgcc ttgtcttccg
      241 gcaaagacac cactgccgcg cgcccggtgc cggcggcgcc ggtttttccc gccaattccg
      301 aaatcgcggc cgccaaactt ttttcgtccc ggatttctcc gctttgcacc gcgccctccg
      361 gcaggcgcaa aaatccggcc gcgacaacga ccaatccgcc gttgcgatgc gccaaacgca
      421 tcattctggc ataggtatcg gcaatctcca gtccgtaaat ttcttttgat aaactcaaaa
      481 aatccagcat ataatctgat aaaaaagtcc ggccgccatt tgtccgcagg gaggaacaac
      541 acggcatttc cgcggaaaaa tagggccgaa ggggcgaccg cgtttctttc tcccttcttt
      601 cattttattc catttcttcc caactgacaa ttttataatc cgatcccatg ctcatcacga
      661 acggcggcgg gttataaacc tgtccctgat ccgaataggt atatctttgc gcgtatccgg
      721 aaaccatttg cccgccgctt gtccattgcg ttcccacccg cccgttgctg atgacggaac
      781 cgcggatcgt caaagaatcc ctgaaattat cctcgtaatg gtttcggccg aaccgtcctt
      841 tttgcgccac aatgaccgca tccaaaacca tatcttcggg agattgaggg ccaatcaaaa
      901 catttttctg gccgatcagc gccaacccgt tggccccgct gtaatcgaca tattggatat
      961 tgccgggaag gacaacgcta gtttccacgc ccgttgagat taaatttgcc gatgccaccg
     1021 taattttgcc gcgcaccgtg ccttcaatcc aaagattatc ctcaaaataa agcaccgcgc
     1081 aatcggaagg aatggtatag ggattgttgg catccgcctg ctcattattg ataataaacc
     1141 agtcttttac atccccttcc tcttcactat atccgctcac ttgctgtagc gccattatga
     1201 aataaacttc aaacttgccg tcttcccgca atttcacgtg ataccctttg cttccgggtt
     1261 tccgatcggc ggcgggcgga aaataaatgc cgccgccgtt taccgcccgc tccttgatct
     1321 gccccaagtc caccgtgaca ccgttgaaat caaacggcgg caccggaaat ttaaacaaat
     1381 ccggagacgg attggcggtg gtggaaaaaa tgcccggaca gacgcattca tccttgaccg
     1441 gacggccgtc caccaattta gtccccgtga cattcaccgt gcaggccgag ggacaatcca
     1501 gggcgcaatc ataaccgttc ttataatagg gataccaaaa atatctccac tggcaacaac
     1561 cgaacgattc cgtgcaaacc cattttccct cgtcatcaat aatcgccgcg ctggtcatca
     1621 atgattggtt gccgccgtcc atacgaatgc cgccgtttga atgataaggg ccgttaattt
     1681 gatggtcgtc gccaatccaa acattggcat tcaaaacata ggaatattta gccaccgatt
     1741 cccggccgta aacggctttt accgttcttt tgaccgccgg ataatccttg gcccatcccg
     1801 acgaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcat aggtttcggc
     1921 cccctgccag gcggcggcgc attgcaggca actgccttgg cacaaattac cgatcttaat
     1981 gccgtcaata aaaaattccg cgtagcgcga ggacgtcccg taataaccct cggtataaac
     2041 tttcacttgg gcgaacttga gatttccgga caaaaacccg ccaatatcgg cactgcaaga
     2101 tcccccctgg gaaaaagaat ggcattcttt cgttatctgt ccggaattaa ccgttcccaa
     2161 aaaataacgg acgcgttcgg tcgtgccgtc gccatcaata tccgcgtaaa aaataagttc
     2221 tttgtcggcg gctttttcaa tcggataagc gccgttgtca ccgtagcgag cctggcggat
     2281 ttctttgatc ataacatcgg cgccccgccg cgcctcatcg gcggcaatcg tttgttcaaa
     2341 agcgaaatca tggattttat agactatcgc caccaaaccg cccgcggcgg ccatcagcaa
     2401 agaaaaaacc gcgaccgccg tcaaggcctc aataatggta aatccccggc cgggacaaca
     2461 tccgaaaata ttctgccggt ccgacagcaa gttttttttc gttttattca ttttgaacaa
     2521 tgcttatcgc ttcgctggta tcgccggcaa tcgtttttga ttggataaat tcctgatacc
     2581 ccaatgccga cactgaaatt tcatactcgc cgtcctgcaa aaaagtgaaa taggtttccc
     2641 cgccgctgtc cgtaatctgg gttttttcat atcccaaagt ttcgtttttg accttgaccg
     2701 tggcggaaaa aaccggcgcc aatgtcacgg catcaaacac acttatcaga aggccgttgg
     2761 gggcctgcaa atacaacttg accgcggcgg caccccccga aggcgcttga accggcattg
     2821 tcggcggatc ggagccaatc aaggcaagcc cctgtcccgg aacaacggaa aaataataac
     2881 tgtcgccgtc aatgccggaa atatcggcgc ggcccgcgcc tcccagatta tgttcctggg
     2941 aatatttata cactttttct ccgttttcgt cctcgccgat gattttactg ccttgcagat
     3001 ggatcacggc gtcgggaacc ggcgttcccg cggaattact ccaaaacact tgccaattca
     3061 aaatttcggg agtcgcttcc ggatccgatg ttgacaactg tgccctaatc ctgatcgccg
     3121 gataaatgcc tttgtccagc cccatcaaat tgatcggcga caccgtcaat cccgcggaat
     3181 tctgccccag atcagcatcg ggaatcaaca tccaatccac accgtcatag tataaaagct
     3241 gatatcggat ttcggtaccc ggcggccgtt ggtcggagaa ttgaatttca ttccatgccg
     3301 ccaaatcgcc gggggcgatt gactgtgaaa cggcgtatcc gcttgaataa ggcggaccaa
     3361 ccaatgtggc attgcccgcc gctatcgcaa cgttgctcgt ttccgccagc aaagattcat
     3421 cgtcaaaatt atcggaaaaa ttaccctgtc cgaccggtgc aatcgcctca acagaaattg
     3481 aagcggttct gtcaattgaa agactcatcg gcgtttcact gtcctgcaat accgtcggat
     3541 gggggctttc cggaacggca acctcatcgg aaccgtaggt ccgcgcggcg ctgtaatcgg
     3601 tttttgaaac ttcaacccgg tagctttggg gagaaagcgg aaaagaatgg cggccggttt
     3661 ccggtgtcgc ggaagcggtt tgaatgccgg tggacagatc aacaatcttg atttcgggag
     3721 acggcaccag actcccgcca tcgtcaaaaa ccgtgaccgt caacactcct cccggctgac
     3781 tttggcacga ttgagcctcc tgaaccttgc t
//
LOCUS       DAOQWE010000075         3800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_24828_length_3800_cov_6.825901, whole genome shotgun sequence.
ACCESSION   DAOQWE010000075 DAOQWE010000000
VERSION     DAOQWE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24828_length_3800_cov_6.825901"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..629)
                     /locus_tag="PKK37_03090"
     CDS             complement(132..629)
                     /locus_tag="PKK37_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF359 domain-containing protein"
                     /protein_id="HNR81397.1"
                     /translation="MPKTYILPEKNRLRMQKVWGKALFGSENEIKQKYAQTLARKKYK
                     TVITVGDYCSHHLQSDIKIFDKKVQRRDFDQKHACSINVKNPAGTIQKESWDAVREAV
                     KKTANLCVDGEEDLLVIPAVILAKPKTLVVYGFPNKGICLLEANLKNKTIFRLVLKRY
                     FTPAN"
     gene            818..>1845
                     /locus_tag="PKK37_03095"
     CDS             818..>1845
                     /locus_tag="PKK37_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNR81398.1"
                     /translation="MFYECQKCKRRWSHPLSECPYCLIVLDKMPESGAKVRGAAKVSI
                     LTLFHPNKPYDVLVLEDGAGNRWGHKSEKEYQIGEEFAIEGNNNPDAVAVWRVKYDQK
                     EAIAKALDLIGGIDAGANAKIAVLPTLEKASHSYFRDNASPEFLAAVLGLLLEKGAKA
                     ENIAVAGQSFGDMPVGAMAQKSGLVAVCQKFGIVPTDLAAGEFIQSGQFEISKTILEA
                     DIFINLSMEKMGRAAACENMFRVLKKESYEAQAYLSSKTAIAAGLAPLLKNMIVIGEA
                     EFVPRSNRLAAFLGLALASRSAANLDRVFGEIAQSFKMPEIIKDIPMETIPLAGRTIK
                     EVQYQAEI"
     assembly_gap    1846..1945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1946..2033
                     /locus_tag="PKK37_03100"
     CDS             <1946..2033
                     /locus_tag="PKK37_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNR81399.1"
                     /translation="AAAIAFLASDQAGFITGAVLNVDGGLWL"
     gene            2113..3618
                     /locus_tag="PKK37_03105"
     CDS             2113..3618
                     /locus_tag="PKK37_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNR81400.1"
                     /translation="MLYNKEQIKEIIPYGEPFLWVDEIEKIEGDTIVGYKQTSPRDDF
                     FAGHFVDFPIMPGVLVVEGIAQTGTLLLRQKIGGSHKNKHLLAYQVRGAQFLAPIMPG
                     DRIKYRVQLLGFYDEKIANFAGEASVDGVLKCEVRFSVAVMDKSGMKEKFLKQGTAAK
                     PTARKECFSLPPLAIGGKTAKYPIIQGGMAVRVSLHNLAGNVAKNGGVGIVAISGMMG
                     GEEVKSEIRQARAIAGPDGIIGVNIMGVIGRFEELLTAALEEGVDLVIQGAGFRLDTF
                     EMAKKYNTPMFSIASSVKVAQKAKDAGAAAIVVEGADAGGHLGFPHGKPFRKTIDILK
                     EIKESGVNLPLIAAGGIFTGADIVEMLRAGAAGVQMATRFVSTEECDANIKFKQAHVN
                     ATKDDVVIIGSPVGLPGRAVRTPFVDKVVAGTAPKALPEECKGCIGPVCNKSYCILKA
                     LEAARKGDLDNGLVFAGANVWRVKEITTVKKLIKELVEEANAILAKEPLII"
BASE COUNT          947 a          788 c         1037 g          928 t
ORIGIN      
        1 tcaatcaata ccaaaagcgg ctgtttcaat ctcaaattga aacagcgcag attgcccaac
       61 agaacctgct ttgattgttt tgttgcggta tcggtcggca taccctgata ctaccaaaac
      121 caatttttcc gtcaattggc gggggtgaag tatcttttca ggacgaggcg gaagatggtt
      181 ttgtttttca ggttggcttc caaaaggcag atgcctttgt tgggaaagcc gtagaccacc
      241 agtgttttgg gctttgccag aatcactgcc gggatcacca gcaggtcttc ctcgccgtca
      301 acgcaaagat tggcggtttt tttgacagcc tcgcgcacgg catcccaact ttctttctgg
      361 atggtgcccg ccgggttttt gacatttatg gagcaggcgt gcttttggtc aaagtcgcgg
      421 cgctggactt ttttgtcaaa tattttaata tcggattgca gatggtgcga gcaataatcg
      481 cccacggtga tcacggtttt gtattttttt cgggccagag tttgggcata cttttgtttt
      541 atttcgtttt ccgagccgaa aagcgccttg ccccagacct tttgcatccg caggcgattt
      601 ttttctggca aaatataggt ttttggcata tactaaacga taaattgcaa gaaccaaatc
      661 ccaagctctc ccgctttgcg ggatctcgcg aagcgagaca aataatattc aatattcaag
      721 tttcaatatt taaaatttga gttttggaaa ttgaaaatta ctttgtgatt tggtgcttga
      781 taattggagc ttaaccaatt tattttacca caagattatg ttttatgaat gccaaaaatg
      841 caagcggcgg tggagtcatc ccttgtccga atgtccatat tgcttgattg tgttggataa
      901 aatgccggaa agcggcgcca aggtgcgcgg tgcggccaaa gtttccattt tgacgctttt
      961 ccatcccaat aagccctatg atgttttggt tttggaggac ggtgccggaa atcgttgggg
     1021 ccacaagtcg gagaaagaat atcaaatcgg cgaagaattt gccattgagg gcaataacaa
     1081 tccggacgcg gtggcggtct ggcgcgttaa atacgaccaa aaagaagcaa tcgccaaggc
     1141 gctggattta atcggcggca ttgatgccgg cgcgaatgcc aaaattgccg ttttgccaac
     1201 gctggagaaa gccagccatt cctatttccg cgacaatgcc tcgccggaat tcttggcggc
     1261 ggttttggga ttactattgg agaagggcgc gaaggcggaa aatatcgcgg tggcggggca
     1321 gagtttcggg gatatgccgg tcggcgcgat ggcgcaaaaa tcgggattgg tcgccgtttg
     1381 ccaaaaattc ggcatcgtgc cgacggattt ggcggcgggc gagttcattc aaagcggcca
     1441 gtttgaaatt tccaaaacaa ttcttgaagc ggatattttt atcaatttgt caatggaaaa
     1501 aatggggcgc gccgcggcat gcgagaatat gtttcgagtt ttaaaaaagg aaagttatga
     1561 ggcgcaagca tatttgtctt caaaaaccgc cattgccgcc ggattggcgc cattgctgaa
     1621 aaatatgatc gtgatcggcg aggcggagtt cgtcccgcgg tcaaaccggc tggcggcgtt
     1681 tttgggatta gcgctggcgt ccagatcagc ggccaatttg gatcgggttt ttggcgaaat
     1741 cgcgcaatcg tttaaaatgc ccgaaattat caaagatatt ccgatggaga ccattccgct
     1801 ggccggccgg acgattaaag aagttcagta tcaagcggaa atcttnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnggctg ccgcgatcgc atttttggcg tcggatcagg
     1981 cgggctttat caccggcgcg gtgttgaatg ttgacggcgg cctgtggctg taagagtgga
     2041 gaaatggctt gccgcaattg cacccggatt gttttcatgg taaaagtatg attcaacgat
     2101 taaattcaat ttatgcttta caacaaagag caaataaagg agattatccc ttacggagag
     2161 ccgtttttgt gggtggatga aattgaaaaa attgaaggcg acacgatcgt gggctataaa
     2221 caaacctcgc cgcgggacga tttttttgcc gggcattttg ttgattttcc gattatgccg
     2281 ggagttttgg tggtagaggg aatcgcccag accggcactt tgctgttgcg ccaaaaaatc
     2341 ggcgggagcc acaaaaacaa acatctttta gcctaccaag tgcggggggc gcaatttttg
     2401 gcgccgatta tgccgggaga cagaatcaaa taccgggtcc agctgttggg cttttacgat
     2461 gaaaaaatcg ccaatttcgc cggcgaagcg tcggtggacg gagtgttaaa atgcgaggtt
     2521 cgtttttcgg tggcggtgat ggataaatcg ggaatgaaag aaaaattttt aaaacaagga
     2581 acggcggcaa aaccgacggc gcgaaaagaa tgtttttctt tgccgccgct ggcgattggc
     2641 ggcaaaaccg ccaaatatcc catcatccaa ggcgggatgg cggtgcgcgt gtcgttgcac
     2701 aatctggcgg gcaatgtcgc caaaaacggc ggcgtgggga ttgtggcaat ctcggggatg
     2761 atgggcggcg aagaagtcaa aagcgaaatt cgccaagccc gcgcgattgc cgggccggac
     2821 ggaataatcg gcgtcaatat tatgggggtg atcggccggt ttgaagaact gctcaccgcg
     2881 gcgctggagg aaggcgttga tctggtgatt cagggcgcgg ggtttcgcct ggatactttt
     2941 gagatggcga aaaaatacaa cacgccgatg ttttccatcg cttcttcggt gaaagtggcg
     3001 caaaaagcca aagatgccgg cgcggcggca attgtggtgg agggggcgga cgccggcggc
     3061 catctgggct ttccgcacgg caaaccgttc cgcaaaacga ttgatattct caaagaaatc
     3121 aaggagtcgg gcgtcaactt gccgttaatc gccgccggcg gaatatttac cggcgcggat
     3181 attgttgaaa tgttgcgcgc gggcgcggcg ggagtgcaga tggcaacgcg ttttgtttcc
     3241 actgaagagt gcgatgccaa cattaaattc aagcaggcgc acgtcaacgc gacaaaagat
     3301 gacgtagtga ttatcggttc gcccgtggga ctgcccggcc gggcggtgcg cacgccgttc
     3361 gtggacaagg tggtggccgg caccgcgccc aaagcgcttc ccgaggaatg caagggatgt
     3421 atcgggccgg tgtgcaataa gagctattgc attttaaagg ccttggaagc ggcgcgcaag
     3481 ggcgatttgg acaacgggct ggtgtttgcc ggcgccaatg tgtggcgggt caaagaaatc
     3541 accaccgtca aaaaattaat caaagaactg gtcgaagaag ccaacgcgat tctggcgaaa
     3601 gagccattga ttatttaaaa ttaaaaaatc aaaatgaaaa atgacaatgc aaaatcgcaa
     3661 aatttgcaaa acgcgacatt ttaacatttt aaattgtcat tttgattttt gatatttaca
     3721 tttttaattt ggaatatgtt cgcaaataac aaactcacca aacttttaaa tatcaagtat
     3781 cccatcatcc agggcgggat
//
LOCUS       DAOQWE010000076         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_25938_length_3690_cov_8.041541, whole genome shotgun sequence.
ACCESSION   DAOQWE010000076 DAOQWE010000000
VERSION     DAOQWE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25938_length_3690_cov_8.041541"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..92
                     /locus_tag="PKK37_03110"
     tRNA            7..92
                     /locus_tag="PKK37_03110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Ser,seq:gct)
     gene            272..621
                     /locus_tag="PKK37_03115"
                     /pseudo
     CDS             272..621
                     /locus_tag="PKK37_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            800..1750
                     /locus_tag="PKK37_03120"
     CDS             800..1750
                     /locus_tag="PKK37_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81401.1"
                     /translation="MSIIQKVIMGAAAVALAVGGYFAANYFSDANQKNTLDTPERIAA
                     DYQNLLDEAKRLEAAAGADCGNKKDINRQIEALENKLADLDARKKQWLDSVPELPDVE
                     LEIRDQNDPNRPGSQVPELSSDVPPLPDVNVEVIPGSEVPELTSDVPPLPEINIELIE
                     SADYIPEMPEIPEINPGRPGSEVPELTSDVPPLPEIDEADIINPDEPIFQMEDAGQKI
                     NNILQRLKALCQEDAPVKKPVSDKCSDACQRHKDCAAFTEDATPADLNDAYSTCMEEC
                     VAWPVEMVKCINAIDINVPNDCVGFLNCQLPQYYEEKYLK"
     gene            1824..>3690
                     /locus_tag="PKK37_03125"
     CDS             1824..>3690
                     /locus_tag="PKK37_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR81402.1"
                     /translation="MDGEIIKGLSSGEAAVRLKSGGYNQLPQQRRQSFWRIFWEVIKE
                     PMLAILVLAGIVYFFLGAPEDALLLSVFIAFTVGITFYQQRKTERALEALRNLSSPRA
                     LVIRDGRRRRIAGREVVVGDVIILNEGDRVPADGLVLAATNLMADESFLTGEAAPVSK
                     SVWNGKAKMGDDRPGGDNRPFIYSGALITRGHGLARTAAIGAATEIGKIGKSLDNIKD
                     GQTVLSREVHGLAKIFGVIGLTLCVMAVGFYWFLHGRFLEGFLYGLTIGMSMLPEEFP
                     VVMVVFLALGAWRISKRKVLTRNPSAIESLGAVEVLCTDKTGTLTINQMRLRSLASPD
                     GIADLADYTGIPSPALSAAHKNILGYALLASQTNPFDPMEREIKNASQTFLFSQRWPA
                     QKWQLVKEYPFSGNLLTMSHVWDTGAGDHYVVAAKGAPESIMELCRLSEDEKKKIGMQ
                     MNILLARGLRLIAAAFCEIPKGRAEVEHQREFDFDFLGFLGFEDPLRPSAAPAVAECR
                     RAGIRVCMITGDYPATACHVARRAGLENPDEFLTGNDLANFSAGELEEKIKNVNVFAR
                     ILPEQKMLLVDAFKESRKLIAMTGDGVNDAPALKAANVGIAMAERGTDVAREAADL"
BASE COUNT          931 a          833 c         1070 g          856 t
ORIGIN      
        1 gccaatggag gaatcgcata gtggcattgc aacgggttgc taacccgtga cggtgttaaa
       61 gccgtccgtg ggttcgaatc ccacttcctc cgccttcggt acgccgtagc tttagcgaag
      121 gcgactttcg gtgcgttgaa attgatgagc ccccgccgtc gggggcgttt tgtttgcatt
      181 cgcttttggg gtaaaatcag ggtaaacatc ccgtaaaagg ctaaaaagaa agttaaaagg
      241 gccggaccct tttaggggat gcccaaacaa tatgaatatt aaacccataa aaaccaaaaa
      301 aaattatcag gaagctctca aaagaattga gtggatttgg ggtgccttgc ctggaacgcc
      361 ggaaagcggc gagctggata ttccggcgac gctggttgaa aaatatgaag aagccaatta
      421 tgcgattgtt gcgccgaatc cggttgaagc gataaagttc cgtatggaac aaatgggaat
      481 ggacaaaaag gatttgacca agattattgg tgccaaccgg gtttcggaat tgttttcaaa
      541 aaaagatcgc tatcgctgaa aatgatccgg agtttgcgcg aaaatcttgg cattcctgct
      601 gattccctga ttggcgtgta aaatggggaa aattatgcaa aacaaagcaa ttcgcaaact
      661 gacccgcatc ggcaaaagat cctataaagc ctagtccttg tattttgtgc gatttggtaa
      721 tttgatacga aaataaacta cgccggggca gagccctgac gtattctggc gggtaaataa
      781 aataacgaaa accaattata tgagcataat tcaaaaggtg ataatggggg cggcggcagt
      841 ggctttggcg gtcggcggtt attttgcggc aaattatttt tccgatgcaa atcaaaaaaa
      901 tactttggac actccggaac gcattgccgc ggattaccaa aatttgctgg acgaagcaaa
      961 gcggctggaa gcggccgccg gcgccgattg cggcaacaaa aaagacatta accggcagat
     1021 tgaggcgttg gaaaacaaac tggcggattt ggatgccaga aaaaagcaat ggctggacag
     1081 cgtgccggaa ttgccggatg tggagctgga aatacgcgat caaaacgatc caaaccgccc
     1141 gggttcccaa gttccggagt taagttccga tgtgccgcca ttacccgatg tcaatgtgga
     1201 agtaattccc gggtcggaag ttccggaact cacatccgat gtgccgccgc ttcccgagat
     1261 aaatatagaa ttgattgaat cagcggatta tattcccgaa atgcccgaaa tacccgaaat
     1321 caatcccggc cggccgggtt cggaagtgcc ggaattaact tccgatgtgc cgccgttgcc
     1381 ggaaattgac gaagccgaca ttatcaaccc cgatgagcca attttccaaa tggaggatgc
     1441 tgggcaaaaa ataaacaata ttttgcaacg gttgaaagca ttatgccaag aagacgcgcc
     1501 ggtaaaaaaa ccggtttccg acaagtgcag tgatgcctgc caaaggcata aagactgcgc
     1561 cgctttcact gaagacgcca ctccggccga tttaaacgat gcttacagta cctgtatgga
     1621 agaatgtgtc gcttggccgg tggaaatggt taaatgcatc aatgccattg acatcaatgt
     1681 tcccaatgat tgcgtcggat ttctgaattg ccaactgcca caatattacg aggaaaaata
     1741 tctcaagtaa ttgaaattcg tattcatggc ggaatttggg cgatggatta aggcggccgg
     1801 atttgataaa ataagagatg ggaatggatg gcgaaattat caaggggttg tcatcgggag
     1861 aggccgcggt gcggctgaaa tccggcggct acaatcaatt gccgcaacag cgccgccaaa
     1921 gtttttggcg tattttttgg gaggtgatca aagagccgat gctggcgatt ttggttttgg
     1981 ccggtatcgt ttatttcttt ttgggcgcgc cggaagacgc gcttttgttg tcggttttca
     2041 tcgcttttac ggtgggtatc actttttatc aacagcgcaa aaccgaacgt gcgctggaag
     2101 ccttgcgaaa tttgtccagt ccgcgggcgt tggtgattcg cgacggccgg cgccggcgta
     2161 tcgccgggcg cgaagtggtg gtcggagatg ttatcattct aaatgaaggg gaccgggtgc
     2221 ccgccgacgg cttggtattg gcggcgacaa acttgatggc cgacgaatcg tttttgaccg
     2281 gggaggcggc gccggtgtca aaatcggttt ggaacggcaa ggcgaaaatg ggagacgacc
     2341 ggcccggcgg cgacaaccgg ccgtttattt attccggggc gctgatcacg cgcggccatg
     2401 ggttggcacg gacggcggca atcggcgcgg ccacggaaat cggaaaaatc ggcaaatcgt
     2461 tggacaatat caaagacggg caaacggttt tgagccgcga agtccacggc ttggcgaaaa
     2521 ttttcggcgt catcggtttg acgctttgcg ttatggcggt gggattttat tggttccttc
     2581 acggccgttt tttggaagga tttttgtatg gcttgacgat cggcatgtca atgcttcccg
     2641 aggaatttcc cgtggtgatg gtggtgtttt tggcattggg cgcgtggcgg atttccaaac
     2701 ggaaagtgct cacgcgcaat ccctctgcga ttgaatcgct gggagcggtg gaggttcttt
     2761 gcaccgataa gaccggcacg ctcacgatca atcaaatgcg cctgcgatcg ttggcgtcgc
     2821 cggacggcat tgcggatttg gccgactata ccggcattcc atcgcccgcg ttgagcgcgg
     2881 cgcacaaaaa tattttggga tatgctctgc tggccagcca gacgaatccg tttgatccga
     2941 tggaaaggga gattaaaaac gcgtcgcaaa cctttttgtt ttcacaacgc tggccggcgc
     3001 aaaaatggca attggtcaaa gaatatccgt tttccggcaa tcttttgacg atgtcgcatg
     3061 tttgggacac cggcgcgggc gatcattacg tggtggcggc aaagggtgcg ccggaatcaa
     3121 tcatggaact ctgccgtttg tccgaagacg aaaagaaaaa aattggaatg cagatgaaca
     3181 ttttgctggc gcgcggcttg cgcttgattg ccgcggcgtt ttgcgaaatt cccaaaggaa
     3241 gagccgaggt cgaacatcag cgcgaatttg attttgactt tttgggattt ctggggtttg
     3301 aagatccttt gcgtccgtcg gcggcgccgg cggtggccga atgccgcagg gcgggcatcc
     3361 gcgtgtgtat gatcaccggc gattatccgg caaccgcgtg ccatgtcgcc cgccgcgccg
     3421 gattggagaa ccccgacgaa tttctcaccg gcaacgatct ggcgaatttt tccgccgggg
     3481 aattggaaga gaaaatcaaa aatgtaaatg tcttcgcccg gattctgccc gaacaaaaaa
     3541 tgcttttggt ggacgctttc aaggagagcc ggaaattgat tgcgatgacc ggcgacggcg
     3601 tcaacgacgc cccggcgttg aaggccgcca atgttggcat tgccatggcc gagcggggca
     3661 ccgatgtggc gcgcgaagcc gccgatctgg
//
LOCUS       DAOQWE010000077         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_27163_length_3573_cov_4.156623, whole genome shotgun sequence.
ACCESSION   DAOQWE010000077 DAOQWE010000000
VERSION     DAOQWE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27163_length_3573_cov_4.156623"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKK37_03130"
     CDS             complement(<1..969)
                     /locus_tag="PKK37_03130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNR81403.1"
                     /translation="MANNDIQNKLWSACNKMRQDPGTTGALQYIEQFSWLLFLKVYEE
                     IENEYEAKAAFEGKTYIRNIDNEFRWSVWTGKDSYITGPDLLEFVQTKLFPYLKNLSG
                     NRTKEIISEIFNNTQNRMEDGYLLREVVDIMAGVDFFGDEDSFAVSSIYEGLFSRMKA
                     SDIKPLAEFHTPRVIARFMTEMVAPKIGQTIYDPCNGPSGFLTEAYHFMRPQEKSTKD
                     KEVLQKETFYGKELKSLPFILGTMNMMLNGIATPNIRKMNTLSINLFNIAEKYDVILT
                     NPPFSGTVRGIESNFPYPSAQTEILFLQHCLRSLKEGGKCAIIFPEG"
     gene            complement(982..3474)
                     /locus_tag="PKK37_03135"
     CDS             complement(982..3474)
                     /locus_tag="PKK37_03135"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81404.1"
                     /translation="MAYNEADTRAKIIDPKIKQSGWTEDFIAREFYFTDGKIFLVGDE
                     AKRKEPKKADYILYYNDALPLAVVEAKEEGKPAVSGMQQAKEYAERLDILFAYSTNGH
                     QIEEFDFVLNTQKTIEAFPTRDELYQRYLTAKSLNNESVAPLATTYNKQTFNANPRYY
                     QQVAIRRALEAITKGQKRILLNLATGTGKTTIAFQIAWKLKQAKQIRRVLFLADRNVL
                     RDQAFNTFEPFGNARGYIEEGQAPTSRDIYFSIYQAMWSEKDGKRIFEQYPKDFFDLI
                     IIDECHRSGFSTWNEILKHFSTATQIGMTATPKQDENINTYKYFGDPQKEYQAVYEYS
                     MAQGIDDGFLANFLLHKVKLNIDKEGVKIEDAVKSGAKIEVPDEAEPNDFYKMEQFEK
                     NIILPDRTEKMCEHLAGLIKTYGEMQKTIVFCVTMEHAGEVAKHLQNHFAHLGFPNYA
                     VRIVSEETDSMALLKTFQDQAQNVPVVATTVDLLSTGFDAPTVQNIVFMKPIRSPIVF
                     KQIMGRGSRISKEKGKFWYRVIDYTNATRLIDEWIEGKPEIKPETPRTNYLNGIVVEE
                     ETLMPIENALMTIQLSANEQTQARTNASGEFSFTNVPEGKIKLYVSANGYKHREITVE
                     TQTSKDDQIQIELTLQKKPVPPIKVHGLTVFVEDEMMFEVDVLGKRLSLKEYVAYSKE
                     EIAKAVKNANDLKAEWTKQDTRDALVAELENQGINIKMLAKITNNTEADEYDLLANLG
                     FGENLHSRQERAEAVLTREQEFLNALNDEQKKIINGLLLKYKENGVMEITKANVFDVY
                     PMPGFMYSQKAFGNPQALRQNVDRLQEKIYAN"
BASE COUNT          904 a          755 c          685 g         1229 t
ORIGIN      
        1 gccctcgggg aaaataatcg cgcattttcc gccttctttg agcgagcgca aacaatgttg
       61 caagaaaagg atttcggttt gcgctgaagg gtacggaaaa tttgattcaa tacctcgcac
      121 cgtgccggaa aatggcggat ttgtgagaat aacatcgtat ttttccgcaa tgttgaaaag
      181 attgatagac aaagtattca ttttgcggat atttggggta gcgatgccgt ttagcatcat
      241 attcattgtt cccaaaataa acggcaagga tttcagctcc ttcccgtaaa atgtttcttt
      301 ttgtaaaact tctttgtctt ttgtgctttt ttcttgtgga cgcataaaat gatacgcctc
      361 ggtcaagaaa ccggacggcc cattgcatgg gtcataaata gtttgtccaa tttttggggc
      421 gaccatttct gtcataaagc gagcaataac gcgcggagtg tggaattccg cgagtggctt
      481 aatatccgac gctttcattc ttgagaaaag cccttcatag atactggaca ctgcaaaaga
      541 atcttcgtcg ccaaagaaat ctactccggc cataatatcc accacttcgc ggagcaaata
      601 gccatcttcc attcggtttt gcgtgttgtt gaaaatttcg gaaataattt ctttggtgcg
      661 attaccagaa agatttttca aatatggaaa aagctttgtt tgcacaaact ctaacaaatc
      721 cggtccggtg atataggaat ctttgcctgt ccaaacgctc cagcgaaact cattatcaat
      781 gttgcggatg taggttttac cctcaaaagc cgcttttgct tcatattcat tttcaatttc
      841 ctcataaacc ttcaagaaca agagccatga aaattgctca atatattgca aagcgccggt
      901 tgtccccgga tcttggcgca ttttattgca cgcgctccat aatttatttt gtatgtcgtt
      961 atttgccata attattttcc gttaatttgc ataaattttt tcctgcaagc gatcaacatt
     1021 ctgccgtaac gcttgcgggt tgccgaacgc tttttgcgaa tacataaagc cgggcattgg
     1081 atatacatca aatacattgg cttttgtgat ttccataacg ccgttttctt tgtatttcaa
     1141 aagtaagccg ttgattattt ttttctgctc atcattcaag gcgttcaaaa attcctgctc
     1201 gcgagtaagc acagcttcag ccctttcttg acgggaatga agattttcac caaaaccaag
     1261 atttgccaag agatcatatt cgtcggcttc ggtattgtta gtgattttcg ccagcatctt
     1321 gatgtttata ccttgatttt ccaattcggc aacaagcgca tctcttgtgt cttgtttagt
     1381 ccattccgcc ttcagatcat ttgcattttt aactgctttg gcaatttctt ctttggagta
     1441 agcaacatat tccttaagcg ataatctttt gcctaaaaca tcaacctcaa acatcatctc
     1501 gtcttcaaca aaaactgtca gcccatgcac tttgattggt ggaacaggtt ttttctgtaa
     1561 agtaagctct atttgaattt ggtcatcttt gctggtttgt gtttcaactg ttatctcgcg
     1621 gtgtttgtag ccgtttgcgg aaacatacaa tttaattttt ccttccggta cattcgtgaa
     1681 agaaaattct ccgcttgcgt ttgttctggc ttgcgtctgt tcgtttgcgg atagttgaat
     1741 tgtcatcaag gcgttttcta tcggcatgag cgtttcttct tcaacgacaa ttccattgag
     1801 ataatttgtt cgcggagttt cgggtttaat ttccggcttg ccctctatcc attcgtcaat
     1861 caatcttgtg gcgttggtgt aatcaattac cctataccaa aatttgcctt tttctttcga
     1921 aatacgactg cctcgcccca taatctgctt gaaaactatc ggggagcgaa ttggcttcat
     1981 aaagacaatg ttttgcactg tcggcgcgtc aaaaccggtg gaaagcaaat cgactgttgt
     2041 tgctacaacc ggaacatttt gtgcttgatc ttgaaaagtt ttgagaagcg ccattgaatc
     2101 cgtttcttct gacacaattc gcactgcata gtttggaaag ccgaggtgcg caaaatgatt
     2161 ttgcaaatgc ttggcgacct cacccgcgtg ttccattgtg acgcaaaaaa caattgtctt
     2221 ttgcatttcg ccataagttt ttataagtcc ggcaaggtgt tcgcacattt tttcagttct
     2281 atcgggcaga atgatattct tttcaaactg ctccattttg taaaaatcat tcggctccgc
     2341 ttcgtctggc acttcaatct ttgctccact ttttacggca tcctcaattt ttacgccttc
     2401 tttatcaata ttgagtttga ccttgtgaag taaaaagttg gcgagaaaac cgtcatcaat
     2461 tccttgcgcc atgctgtatt cataaaccgc ttgatattct ttctgcggat cgccaaaata
     2521 tttatatgtg tttatatttt catcttgttt tggcgttgca gtcattccaa tttgtgtagc
     2581 agttgaaaag tgcttcaaaa tttcattcca tgtgctgaaa ccgcttctgt gacattcatc
     2641 tataataatc aaatcaaaaa aatcttttgg atattgttca aaaattcttt tgccgtcttt
     2701 ttcgctccac attgcctgat agatactgaa ataaatatct cttgaagtcg gcgcttgtcc
     2761 ttcctcaatg tatcctcgtg catttccaaa gggttcaaaa gtgttaaacg cctgatccct
     2821 taaaacattt ctgtcagcca agaaaagcac acgacgaatt tgttttgcct gtttcagttt
     2881 ccatgcaatt tgaaaagcga ttgttgtttt tcctgttccg gtggcaagat taagcaggat
     2941 tcttttctgt ccttttgtga ttgcttccaa cgcgcgacgg atagcaacct gttggtaata
     3001 acgaggatta gcgttgaaag tctgtttgtt gtaagtagtc gccaatggag caaccgattc
     3061 gttgtttaga gattttgcag tcaaatatct ttgatatagc tcatcccgcg tcggaaaagc
     3121 ctcaatcgtc ttttgagtgt tcaagacaaa atcaaattcc tcaatttgat gcccgtttgt
     3181 tgagtaagca aaaagaatat ctaatctttc ggcatattct ttagcttgtt gcattccgga
     3241 aactgccggt tttccttctt cttttgcttc tacaactgcc aaaggtaggg catcattgta
     3301 atataaaata tagtcagctt ttttgggttc ttttcttttt gcctcatcgc caacaaggaa
     3361 tatttttccg tcggtgaaat aaaattcacg cgcaataaaa tcctcagtcc agccggattg
     3421 ctttattttt ggatcgatta tttttgctct tgtatctgct tcgttgtatg ccatattttt
     3481 atttgattaa atcatcaacg ccaacaccaa gcgctttagc gattttggat agcgtttcta
     3541 tggttgggtt gatattcgct cccgattcaa tct
//
LOCUS       DAOQWE010000078         3552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_27388_length_3552_cov_6.678010, whole genome shotgun sequence.
ACCESSION   DAOQWE010000078 DAOQWE010000000
VERSION     DAOQWE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27388_length_3552_cov_6.678010"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PKK37_03140"
     CDS             <1..1055
                     /locus_tag="PKK37_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNR81405.1"
                     /translation="GLLSIYSNLSRAGDFSAFWRQAAFLGIGVAAMLAASFFDWRIFK
                     ENSAAVLMFYAASIIALAGLFFFGSSVRGVRTWYDLGIFTLDPIELLKPALLALLAKY
                     FSRRHETMYQIRHLALTGLYAALPAALIFFQPNLGPCLVIIPLWIGLLLMAGAKKTHL
                     LFLLAGFLALFVLGWMFLFKDYQKDRIADFFAPKDPLGVSWSQNQARIAVGTGGILGK
                     GFGQGSQAQYGFLSEPQTDFIFAAIIEEFGLLAGAGALFLLFMIVWRLTRAARRARDN
                     FSKFFLAGLAMLLFAEAAVHVGVNIGFLPIIGLPLPLVSYGGSNLISTLALLGLAQGM
                     ISGRGILPSENERQTD"
     gene            1159..3450
                     /locus_tag="PKK37_03145"
     CDS             1159..3450
                     /locus_tag="PKK37_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR81406.1"
                     /translation="MSNNFSKFTVYAALFLVPLFTLPFTANVLDFPKQFLLLFLTIIG
                     FVFWLWAAVSERKFHINLNPINLFPLGLIAAVFVSGVFSLYRYGSFWGWPLPVAESFA
                     AVASFGLLYFLVVNNFRKSDLPDLIGVLGISAAIAAVYGVLQAFGIYLAPFLSYAKDN
                     PAFNTVGSTGGFLVFLAIVLAMILPLAFAAKNKWAIPMKICAAILFATLVFFNGALTI
                     YLPSKAGAAGYDFSLAPWIALGTGMLSLAIFAINNQKFSERNRGMKNTASILFFCAVL
                     FSVFNIFARPIVLQISENLGKNIGGQIGSEIALRQTNSAGVAVETLKQSPREFFLGSG
                     PGTFGYDYVKFRPKQIAQDNLGWNLTFFSGSSEFLNRVATTGVLGGLFFLLLILAWII
                     EGFRVLVREEEKIFLPLAIFSGWLAIAVAAFYYPFNLALMMLFWFLLAAVVAINQEKT
                     VLFPLDTIKKNYAASMIFVVLLIGAIALMVQSAKNYYAEVAYQEAVEAFAKKDIAAAI
                     QKLNIAADATNRLQDNYLTGLAQAYWAQAEQEIEQKKDQEAQVALQAAAPYLQEAVKN
                     AVQSTEIANPNNSFNWAFRAQIYRKLIGVDEGFDDWALNMYEKAIKLETSNPTLYNEV
                     GQVYLIKNDLEKARIAFEKAVELMPQYIDAHYYLALIADKKGDRTAAIGHLEVVGQLL
                     PADDAASRENIEKAIDTLRRGGSISSPTAESAPAVPPASGDELSPASGSENAAEGAPA
                     SGLENAESAPEDVLPPEPAAVND"
BASE COUNT          715 a          863 c         1023 g          951 t
ORIGIN      
        1 tggggttgct ttccatttac agcaatttgt cgcgcgcggg cgacttttcc gctttttggc
       61 gccaggcggc gtttttggga attggcgttg ccgcgatgct ggcggcgagt ttttttgatt
      121 ggcggatctt caaggaaaat tcggcggcgg tgctgatgtt ctatgcggcg tcaatcatcg
      181 cgctggcggg attgtttttt ttcggctctt ccgttcgcgg cgtgcggaca tggtatgatc
      241 tgggcatttt cactcttgat ccgattgaat tgctcaagcc cgcgctgttg gcgctgttgg
      301 cgaaatattt ttcccggcgg cacgaaacga tgtatcagat ccggcacttg gcgctcaccg
      361 ggctctatgc ggctctgccc gccgcgctga tttttttcca gccgaatttg ggaccgtgtc
      421 tggtgatcat ccctctctgg atcggcctgc tgttgatggc gggcgcgaaa aaaacgcacc
      481 tgcttttcct gctggcggga tttttggcgc tttttgttct gggatggatg tttttgttca
      541 aagactacca aaaagaccgg attgcggatt ttttcgcgcc caaagatccg ctgggggttt
      601 cgtggagcca aaatcaggcg cgcatcgccg tgggcaccgg cggcattttg ggcaagggtt
      661 tcgggcaggg atcgcaggcg caatacggtt ttctgtccga accgcaaacc gattttattt
      721 tcgcggcgat cattgaagaa ttcgggcttt tggccggcgc cggggcgctg tttctgttgt
      781 ttatgattgt ctggcgcttg acgcgggccg cgcggcgcgc tcgcgataat ttttcaaaat
      841 tcttcttggc gggattggca atgctgttgt tcgcggaggc ggccgtccac gtcggagtga
      901 acatcggctt tttgcccatt atcggcctgc cattgccgct ggtgagctat ggcggatcaa
      961 acttgatttc aacgctggca ctgctgggct tggcccaggg aatgatttcc ggcagaggca
     1021 ttttgccgtc cgaaaacgaa cgccaaaccg attgatttct tttgccgttt tggtttataa
     1081 taaaatcaat gatcaattat cacttatcat tgatcggtaa tcaattacgt aattcaaaaa
     1141 aataataaaa gcaaaaaaat gtccaacaat ttttccaaat tcacggtcta cgcggcgctc
     1201 tttctggttc ccttgttcac cttgccgttc acggccaacg ttctggattt tcccaaacaa
     1261 tttttgcttt tgtttctcac gatcatcggg tttgttttct ggctctgggc ggcggtgagc
     1321 gaacgaaaat ttcacatcaa tctcaacccg atcaatctct ttcctctcgg cctaatcgcc
     1381 gcggttttcg tttccggcgt tttttctctt tatcgctacg gcagtttttg gggctggccc
     1441 ttgccggtgg cggaaagctt tgccgcggtg gcgagtttcg gcctgcttta ttttttggtg
     1501 gtcaataatt tcagaaaatc cgatctgccc gatttgatcg gcgttctggg aatttcggcg
     1561 gcgattgccg cagtctatgg cgttttgcag gcttttggca tctatctggc gccctttttg
     1621 tcctatgcaa aagacaaccc ggcgtttaac accgtgggat caaccggcgg gtttttggtg
     1681 tttctggcga tagtgctggc gatgattttg ccgctggcgt ttgccgcgaa aaacaaatgg
     1741 gcgattccga tgaagatatg cgccgcgatc ctgtttgcca cgttggtttt tttcaacggc
     1801 gcgctcacca tttacctgcc atcaaaggcg ggtgccgcgg gatatgattt ttccctggcg
     1861 ccgtggatcg ctctgggaac gggaatgctt tctctggcga tctttgccat caacaaccaa
     1921 aagttttcgg agagaaaccg gggaatgaag aacaccgcca gcattttgtt cttttgcgcg
     1981 gtgctgtttt ccgttttcaa catttttgcc cggccgatcg ttttgcagat ttcggaaaat
     2041 ttgggcaaaa atatcggcgg gcagatcggt tcggagatcg ctctgcggca gacgaattcg
     2101 gcgggagtgg cggtggaaac tttaaaacag tctccgcggg aatttttcct cggctccggc
     2161 ccgggcactt tcggctatga ctacgtgaaa ttcagaccca aacagatcgc gcaggacaat
     2221 ttgggatgga acttgacgtt cttttccggg tcctcggaat ttctcaaccg cgttgccacc
     2281 accggcgttt tgggcggctt gttcttcctt ctgctgatac tggcgtggat tattgagggg
     2341 tttcgcgttc tggtgcggga agaggaaaag atattcttgc cgctggcgat tttctccggc
     2401 tggctggcga ttgccgttgc cgcgttttac tatccgttca atctggcgct gatgatgctt
     2461 ttctggttcc tgctggcggc ggtggtggcg ataaatcagg agaagacggt gttgttcccg
     2521 ttagacacca tcaagaagaa ttacgccgcg tcaatgatct tcgtggtttt gctgatcggc
     2581 gcgattgcgc tgatggtgca aagcgccaaa aattattacg cggaagtcgc ctatcaagag
     2641 gcggtggagg cgttcgcgaa aaaggatatt gccgcggcga tccaaaaact caacattgcc
     2701 gccgacgcca caaaccgttt gcaggacaac tatttgacgg ggctggcgca ggcctattgg
     2761 gcgcaggccg agcaggagat tgaacaaaaa aaggatcagg aggcgcaagt cgccctgcag
     2821 gccgccgcgc cctatttgca ggaggcggtg aaaaatgccg tccagagcac ggagatcgcc
     2881 aatcccaaca attctttcaa ctgggcgttc cgggcgcaga tctatcgcaa attgatcggc
     2941 gttgacgaag ggtttgacga ctgggcgctg aatatgtatg aaaaagcgat caagctggaa
     3001 acttccaatc cgacgcttta caacgaagtg gggcaggtct atctgatcaa aaatgatttg
     3061 gaaaaagcca ggattgcgtt tgaaaaagcg gtggaattga tgccgcaata cattgatgcc
     3121 cattattatc tggcgctgat tgctgacaag aaaggagatc ggacggcggc aatcgggcat
     3181 ttggaggtcg tcggccagtt actgccggcc gatgacgccg cttcccggga aaacattgaa
     3241 aaggcgattg acactttgcg ccgcggcgga tcaatctcgt cgccaaccgc ggaatcggcg
     3301 ccggccgtcc cgccggcaag cggggacgaa ctttcgccgg caagcggttc ggaaaatgcc
     3361 gcggaaggcg cgccggcaag cggtttggaa aacgcggaga gcgcaccgga ggatgttttg
     3421 ccgccggaac ccgccgccgt aaacgattga cgcgccgcgc gagtttagcg tgattggcgg
     3481 attgccgttc ttttgaaagc gctttaaaga gaaacgatga gcattggaaa atgccaacaa
     3541 tttttgcgat tt
//
LOCUS       DAOQWE010000079         3519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_27749_length_3519_cov_3.664838, whole genome shotgun sequence.
ACCESSION   DAOQWE010000079 DAOQWE010000000
VERSION     DAOQWE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27749_length_3519_cov_3.664838"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKK37_03150"
     CDS             <1..314
                     /locus_tag="PKK37_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81407.1"
                     /translation="WGPDFLPYDFSDLFLDYIRKEINESYNNGQLLAVIVLSRKLLEM
                     IVIRIFEVVFPKISNGNHNESNHELWYDKSRGRHQGFEILLKNLKLKSNDFQEDKELV
                     V"
     gene            712..3393
                     /locus_tag="PKK37_03155"
     CDS             712..3393
                     /locus_tag="PKK37_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81408.1"
                     /translation="MALHKDFPISPYEILDPAIRWFPADENLRDKGYEKLLPPLVAEL
                     RKKVAAWRAAGYEGASETSKALLAWWFLTDHPVENKDGELSLFRYYFAQREAVETVVW
                     LYEVAKSRDKYDLIRYDSSGAVSAGMFDEDWTRYVIKMATGAGKTKVLSLMLAWSYFH
                     KLYEADSNLARNFLIITPNIIVLDRIRADFDGLKIFFQDPILPDNGYCGQNWRDDFQL
                     TLHIQDEIGTVRKTGNIFLTNIHRVYEGATEAAGFDDQDTADYFLGKKPVGATNASKL
                     DLGDIVRNVDELVILNDEAHHIHDPRLAWFKSVGDIHNRLKMKGGALSLQIDVTATPK
                     HNNGSIFVQTVSDYPLVEAIHQNVVKHPVLPDSASRAKLQEHKSSNYTEKYKDYIHLG
                     YLEWQKVYEEHKKLGKKAVLFLMTDDTKNCDDVAQYLENTYQDLKGAVLVIHTKNNGE
                     ISESASGKKEEELKELRQAANAIDSMGSPHKVIVSVLMLKEGWDVRNVTTIVGLRAYA
                     AQSNILPEQTLGRGLRRMYRDPDLPEFVSVVGTDAFMDFVESIKSEGVELEHRKMGEG
                     SGPKSPIVVEIDRENMKKDIEQLDIEIPVLTPRIYREYKNLSGLVCANFDYRKIELKE
                     FSEEEKREIVFRDIASGEITHKTELDSNFIPNYQSAIGYFAQVIMKELRLVSGYDILY
                     GKVKEFIQSYLFNNPVALDNLNSLRNLSEIEASRTVIETFKKEINKLTVLDKGEAEIR
                     DYIKISKSRPFVVKDQGYIVPKKSIFNKVVGDSHFELEFAGFLENCDDIISYAKNYFA
                     VHFKIDYKNADGNISEYYPDFIVKVSPKEYYIVETKGREDLDDIEKIKRLEQWCVDVN
                     SSQKNAKYQMIYIKQEDWEAQGQKPRNFQAVIRGWSK"
BASE COUNT         1100 a          629 c          818 g          972 t
ORIGIN      
        1 tttgggggcc tgattttctt ccatatgatt tttccgattt atttcttgat tatatccgca
       61 aagaaataaa cgaatcctat aataatggcc aattgttggc tgttattgtc cttagtcgaa
      121 aattgttaga aatgatcgtt attcgtattt tcgaagttgt ttttccgaaa atatccaatg
      181 gaaatcataa tgaaagcaat catgaacttt ggtatgataa aagcaggggt cgccatcaag
      241 gattcgaaat tttattaaag aatttaaaat taaaatcaaa tgattttcaa gaagacaagg
      301 aattggttgt gtaaacatcc gatgcaatgg atgcaataag aaatcaagca aataaatgcg
      361 ttcatcgcga ttataaaatt ccagatgaaa attatttgaa aaatcttaag atagaagctg
      421 cagtggtcgc aatacgtaaa atttacaaaa aatattgcaa tccataaaaa ttgacgaaag
      481 aagtttattt gcacagggca aaacaaataa cggtaaaaat gattagttta agcgcaaaat
      541 aagaagttta tttgcgttga gggggcgttt tgccgataac aaaacagccc tcctcgcgga
      601 aggttgttct tggatgtaat atccttccag tttatggaaa ctgaaagaca attattatat
      661 atcacataat tacggatcca aaacaagatt tttacacaaa ctaattcaac tatggcactg
      721 cataaagatt ttccaatttc gccttacgaa attcttgatc cggctatccg gtggtttccg
      781 gcggatgaaa acttgcgcga taagggttat gaaaaacttt tgccgccgct tgtcgccgaa
      841 cttcgcaaaa aagtcgcggc ttggcgcgcg gcgggctatg aaggcgccag cgaaaccagc
      901 aaagcattgc ttgcttggtg gtttttaacc gatcatccgg ttgaaaataa agatggcgaa
      961 ttatcgcttt ttagatatta tttcgcccag cgcgaagcgg tggaaaccgt ggtttggctt
     1021 tacgaggttg ccaaatccag ggataaatat gatttaatcc gatatgacag ttccggcgcg
     1081 gtttcggcgg gaatgtttga tgaggattgg acgcgctatg tgattaaaat ggcgaccggc
     1141 gcgggcaaaa ccaaggtttt gagcttgatg ctggcgtgga gctattttca taaactatat
     1201 gaagcggatt ccaatttggc ccggaacttt ttaattatta cgccgaatat tatcgtgctt
     1261 gatcgtattc gcgccgattt tgacggattg aaaatttttt ttcaggaccc gatattgccg
     1321 gacaatggat attgcggcca aaactggcgc gatgattttc aattaacatt acatatccaa
     1381 gatgagattg gtacggtaag aaaaaccgga aacatttttt taacaaacat acaccgcgtc
     1441 tatgaaggcg caaccgaagc cgcaggcttt gacgaccaag atactgccga ctattttctg
     1501 ggcaagaaac cggtaggcgc gaccaatgca tcaaagcttg atctgggtga tattgtccgc
     1561 aatgttgatg agttggtaat tttaaatgat gaggcgcatc acattcacga tccgcgtctg
     1621 gcgtggttta aatctgtggg tgatattcac aatcggctga aaatgaaagg cggcgcgttg
     1681 tcgttgcaga tagatgttac cgctacgccc aaacacaaca acgggtcaat ttttgtgcag
     1741 accgtaagcg attatccgct ggttgaggcg attcatcaaa atgtggttaa acatccggtt
     1801 ttgcccgatt ccgccagccg cgccaagctt caagagcata aatcatcgaa ctataccgaa
     1861 aaatacaaag attatatcca tcttggatat ctggaatggc agaaggttta tgaggaacac
     1921 aaaaagcttg gcaaaaaagc ggtgcttttc ttgatgaccg atgacacgaa aaattgcgac
     1981 gatgtggctc aataccttga aaatacttat caagatttga aaggcgcggt tttggtgatt
     2041 cacacgaaaa acaacggcga aatttccgaa agcgcgagcg gtaaaaaaga agaagaatta
     2101 aaagaattgc gccaagccgc caacgcgatt gactcaatgg gaagtccgca caaagttatt
     2161 gtgtcagtgt tgatgctcaa ggagggttgg gatgttcgca atgtaacgac gattgtcggt
     2221 cttcgcgcat atgcggctca aagcaatatt ttgccggagc aaacgcttgg gcgcgggttg
     2281 cgccgaatgt atcgcgatcc ggacttgccg gaatttgtca gcgtcgttgg aacggatgcg
     2341 tttatggatt ttgtggaatc aattaaaagc gagggcgttg agcttgagca tcgcaagatg
     2401 ggggaaggct ccggaccaaa atcgcccatt gtggttgaaa ttgatcggga aaatatgaaa
     2461 aaagacattg agcaattaga catagaaatt ccggttttaa ccccgcgtat ttatcgggaa
     2521 tataaaaacc tttcgggttt agtttgtgcg aattttgatt ataggaaaat cgagcttaaa
     2581 gaatttagcg aagaagaaaa gcgtgaaatt gttttccgcg atattgccag tggcgagatc
     2641 acgcataaaa ccgaattgga cagcaatttt atcccaaatt atcaaagcgc aatcggttat
     2701 ttcgcgcagg tgattatgaa agagcttcgc ttggtcagcg gctatgatat tctttatggc
     2761 aaagttaaag aatttatcca gagctatttg tttaataacc cagtggcgtt ggataatctt
     2821 aattcattgc gcaatctttc cgaaatcgag gcttcaagaa ccgttattga aacattcaaa
     2881 aaagaaatca ataaattaac ggtgcttgat aagggcgagg ctgaaatccg cgattatatc
     2941 aaaataagca aaagccgccc atttgtggtt aaagaccagg gctatatcgt ccccaaaaag
     3001 agcattttca ataaagttgt cggtgacagc cattttgagc ttgaatttgc cggctttctt
     3061 gaaaattgcg atgacataat ctcatatgcc aaaaactatt ttgcagttca ttttaaaatt
     3121 gattataaaa atgccgatgg caatatttcc gaatattacc cggattttat cgtcaaagta
     3181 tcacccaaag aatattacat tgtggagacc aagggtcggg aggatttaga cgacattgaa
     3241 aaaataaagc gtcttgagca atggtgtgtt gatgtaaact ctagtcagaa aaacgccaag
     3301 tatcaaatga tttatataaa gcaagaagat tgggaagcgc aaggccaaaa accaagaaat
     3361 tttcaagcag taattcgggg ctggagcaaa taagcggatt acaattttat gtctgaatca
     3421 gagggttaga ccctctgatt ttgttttttt ggtttgctcg gtatataaaa ttgggggggt
     3481 atcggtggct ttgggggctt gatgcgggtc aagccccca
//
LOCUS       DAOQWE010000080         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_27873_length_3508_cov_8.287286, whole genome shotgun sequence.
ACCESSION   DAOQWE010000080 DAOQWE010000000
VERSION     DAOQWE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27873_length_3508_cov_8.287286"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3484)
                     /locus_tag="PKK37_03160"
     CDS             complement(<1..3484)
                     /locus_tag="PKK37_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR81409.1"
                     /translation="MAKNNMEQNYKEKEGKIIKFWKDNLIFEKSVKREPKTRKDFVFY
                     EGPPTANAKPGIHHVETRVFKDMICRYKTMRGFRVRRRAGWDTHGLPVELQIEKKLGL
                     KNKKEIEQYGIGKFNRECRNSVFEQIGEWRGLTERIGYWLDMDNPYITYDSRYMESVW
                     FVLKRAWEKKLLFQDYKVVPYCARCGTALSSHEVAQGYQKVKDLSVYVKFPVVDPEFK
                     NTALLVWTTTPWTLPANVAAAVNPELEYVKAAPKAGPGEALILAKNRFAIIQDDYAIA
                     EEMSGRNLIGLRYQPPFPIKDEKAPAAYQTIGADFVSAEDGTGIVHIAPAFGAEDMET
                     IKKENRCRTKAHQPEFPVVLNVGEDGGFLPEVSQWAGMFVKDADPLIVKDLEGRRLLL
                     KTELYEHDYPFCWRCKTPLLYYAKKSWFIKMTSLRQDLLKGNEKINWVPAHLKEGRFG
                     EWLREVKDWAISRDRYWGTPLPVWQCDCGHREVFGSAGDLLGQKYSNNRYFVFRHGHS
                     QRQVKKVASCWPEPAPLPLTAKGEKEVRVSAKKLAQEKIDLIVASDLLRTKETARIIA
                     ETTGAKIIHDKRLREANVGIFNGRDPKNFWDYLAEQKNRFTAKVPKGESLQNIRRRTY
                     ELIAEIDKKYEGKNIVLVSHELPLTILEHTLKGVPAPKILDGRHRGKIKKIETGKFRE
                     IEFKQLPFGDDMETDLHRPYIDNIRPLCPNCGQRMARVPEVLDCWLDSGSMPFAQAGW
                     PWLGKAALREAVASAKAPKLFPADFICEAIDQTRGWFYTLMAVSASLGFGAPYKNVVS
                     MGHVLDEKGEKMSKSKGNVINPWEMADKYGADAVRWYFYTVNDPGDAKLYAEKDLELA
                     RKKFLLTLWNCHLFLKTYAPKAKPSAAVSKNVLDRWILSRLNRTIGEISVQLENYGVV
                     AAARKIEDFAINDFSLWYVRRSRRRLQHPQSAAELKAAAGVFAAVLENLARMCAPFVP
                     FLSEEIYQDLSGAGFGKGKSVHLEAWPAPLKKFIDDDLEAGMEEARRLVAWALAERAK
                     RGVKVRQPLAKLVLREKNLPGGLRDLVKEEINVKEIVFDAGLPVFAALDWEITPELEE
                     EGAVREIIRQIQELRKSARFTPADKIAVYVQSDGELGETAAKNQLIIAKETKSAGLKF
                     QRPAKTAAEAEIKIG"
BASE COUNT          779 a         1019 c          797 g          913 t
ORIGIN      
        1 cgccgatctt aatctccgct tccgccgcgg ttttggccgg cctctggaat ttcagccccg
       61 cggatttggt ttctttggcg attatcagtt ggttcttggc cgccgtttcg cccaactccc
      121 cgtcggattg gacataaacg gcgatcttgt ccgccggggt aaaccgggcg cttttgcgca
      181 actcttgtat ttggcggata atctcgcgca ccgcgccctc ttcctccaat tccggagtga
      241 tttcccagtc aagcgcggca aaaaccggca aaccggcgtc aaaaacaatt tctttcacat
      301 tgatttcttc tttcaccaga tcgcgcaagc cgcccggcaa attcttttcc cgcaaaacca
      361 attttgccag cggctggcgc accttaacgc cccgcttggc gcgctcggcc agcgcccacg
      421 ccaccaggcg gcgcgcttct tccattcccg cctcaagatc atcgtcaatg aattttttca
      481 aaggtgcggg ccacgcttcc aaatgcacgg acttgccttt gccaaacccg gcgccggaca
      541 aatcctggta gatctcttcg cttaaaaacg gaacgaaagg cgcgcacatt ctggccaaat
      601 tttccagcac ggcggcaaaa actcccgccg ccgctttcaa ttcggcggcg ctttgcgggt
      661 gctgcaagcg gcggcgggac cggcgcacat accacaggga aaaatcattg atcgcaaaat
      721 cttcaatttt tcgggccgcc gcgacaacac cgtaattttc caactgaacg gaaatttcgc
      781 caatggttcg gttcagccgc gagagtatcc accgatccaa aacattcttg gaaacggcgg
      841 cggacggttt cgctttcggc gcgtaggttt tcaaaaacaa atggcagttc caaagagtca
      901 gcaaaaattt tttgcgcgcc aactccaaat ccttttccgc gtaaagcttg gcgtcgccgg
      961 ggtcgttgac ggtgtagaaa taccagcgca ccgcgtcggc gccgtattta tcggccattt
     1021 cccagggatt gatcacattg ccttttgact tggacatttt ttcacccttt tcgtccaaaa
     1081 cgtgtcccat tgacaccaca tttttgtagg gcgcgccgaa tcccaacgac gcggacaccg
     1141 ccattaaagt gtaaaaccag ccgcgcgttt gatcaatcgc ctcgcagatg aaatccgcgg
     1201 gaaataactt tggggccttc gctgaagcta cggcctcccg aagggcggcc ttaccaagcc
     1261 acggccagcc cgcttgcgcg aagggcattg atcccgaatc cagccagcaa tccaaaactt
     1321 ccggcacgcg cgccattctt tgtccgcaat tcgggcaaag cggcctgata ttatcaatat
     1381 acggccggtg caggtcggtt tccatgtcat cgccaaacgg caattgtttg aattcaatct
     1441 cgcgaaactt gccggtctca attttcttga tttttccccg atggcggccg tccaaaatct
     1501 tcggcgccgg cacgcctttt aatgtgtgct ccaaaattgt aagcggcaac tcgtgcgata
     1561 ccaaaacaat gtttttgcct tcatattttt tgtcaatttc ggcaatcaac tcatatgttc
     1621 gccggcggat attttgcaaa ctctctccct taggcacttt ggcggtaaag cgattttttt
     1681 gttccgccag ataatcccaa aaatttttgg ggtcgcggcc gttgaaaatg ccgacattgg
     1741 cttcgcgcaa ccttttgtca tggatgattt tggcgccggt ggtttcggca attatccgcg
     1801 cggtttcttt cgtccgcaac aagtccgaag cgacaatcaa atctattttt tcttgggcca
     1861 gtttttttgc cgaaactcgg acttcttttt cgcctttcgc ggtgagcggc aagggcgccg
     1921 gctccggcca acaggaagcg acttttttaa cttgccgttg agaatggcca tggcggaaaa
     1981 caaaataacg gttgttggaa tatttttgcc cgagcagatc gcccgcggat ccaaaaactt
     2041 cccgatggcc gcaatcgcat tgccacaccg gcaggggcgt gccccaatag cggtcgcgcg
     2101 aaatcgccca atccttcacc tcgcgcaacc actccccaaa ccgcccttct ttcaagtgcg
     2161 ccggcaccca attgattttt tcgttgcctt tcaacaaatc ttgccgcaac gacgtcattt
     2221 tgatgaacca gctttttttg gcatagtata aaagcggcgt tttgcaccgc cagcaaaacg
     2281 gataatcatg ttcatagagt tcggtcttga gcaatagccg cctcccttcc aaatctttaa
     2341 cgattagcgg atccgcgtct ttaacaaaca ttcccgccca ttgggaaact tcgggcagaa
     2401 acccgccgtc ttcgccaacg tttaaaacca cgggaaattc cggctggtgg gcttttgtcc
     2461 ggcagcggtt ttccttttta atcgtttcca tatcttcagc gccgaacgcc ggcgcgatat
     2521 ggacgatccc cgtgccgtct tccgcggaaa caaaatccgc accgatcgtt tgataggcgg
     2581 caggcgcttt ttcatctttt atcggaaacg gcggctgata gcgcaatccg atcaaattcc
     2641 gaccgctcat ttcttccgcg atcgcataat catcttgaat gatcgcaaaa cggtttttgg
     2701 ccaaaatcaa cgcctctccc ggtccggcct tgggcgccgc ttttacatat tccagctccg
     2761 gatttaccgc cgcggccacg ttggccggca atgtccaggg agtggtggtc caaaccagca
     2821 acgcggtatt tttaaactcc ggatcaacaa cgggaaattt aacatagacc gaaagatctt
     2881 tcactttttg ataaccttgc gccacttcgt ggctggagag cgccgtgccg caacgagcgc
     2941 aatagggcac caccttatag tcttggaaca aaagcttctt ttcccacgcg cgcttcaaaa
     3001 caaaccagac cgattccata tagcgcgaat cataagtgat atagggattg tccatatcca
     3061 gccaatagcc gatgcgttcg gtgagtccgc gccattcgcc gatttgttca aacaccgaat
     3121 ttctgcattc gcggttgaat tttccaatac cgtattgctc aatctctttc ttgtttttca
     3181 gccccaattt tttttcaatc tgcaattcca ccggcaggcc gtgcgtgtcc cagccggcgc
     3241 gccggcgcac ccgaaaaccg cgcattgttt tgtagcggca aatcatatcc ttaaacaccc
     3301 gcgtctccac atgatggatt cccggtttgg cattggccgt gggcggcccc tcatagaaca
     3361 caaaatcttt ccgtgttttg ggttcgcgct tgaccgattt ttcaaaaatc aaattatctt
     3421 tccaaaattt gataattttc ccttcctttt ctttataatt ttgctccata ttgttttttg
     3481 ccacaacttc aatgtagcaa atctggcc
//
LOCUS       DAOQWE010000081         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_27874_length_3508_cov_8.276861, whole genome shotgun sequence.
ACCESSION   DAOQWE010000081 DAOQWE010000000
VERSION     DAOQWE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27874_length_3508_cov_8.276861"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="PKK37_03165"
     CDS             <1..486
                     /locus_tag="PKK37_03165"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81410.1"
                     /translation="PAKVVCLVDAPIGDSTHEERMQICLTAKNNGADELDVVASLRDI
                     KHERWEQVYKDLAEICKILPTKVIIGSGYLTDKEIAMVSRLVKQAGAICVKTATSKDP
                     LEERELKEKACHLEIMKKNAPGLLIKASGNVKTAKDARAYVKAGADIIGTSNGVAIAR
                     G"
     gene            626..2134
                     /gene="gatB"
                     /locus_tag="PKK37_03170"
     CDS             626..2134
                     /gene="gatB"
                     /locus_tag="PKK37_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNR81411.1"
                     /translation="MEYVPTIGLEIHCELKTASKMFCSCPNRPDTGAPNTNVCPVCLG
                     HPGTLPVANREAIRLTRLVGLALDSRILEHSKFDRKNYFYPDLPKGYQISQYDQPLCS
                     GGYLEISDGGKPKKIGITRVHLEEDTGKLMHSDDKDHSLVDFNRGGVPLMELVTEPDI
                     ENADQAREFVKELQLILRHLGASDADMEKGQLRVEVNISLGRIAPDGKKKLGTKVEIK
                     NLNSLKAVEGSVAFEIGRQTARIKNGEKVVQETRGWNEFTGQTFSQREKEEAFDYRYF
                     PEPDIPPLAISPGEIEKLKAAVGELPAKKRFRLAEQYGLGAAIVEIFVVNRDLGEYFE
                     KIASELEEWVNVKEGDCENSDVQNHQLARLCANYLTTDVLGALAGKSFCEKEFPVSPE
                     NFAEFICLINKGEITSKIAKQVLAEMMKTGNDPSAIITQKGLSQIRDEKQIAAMIDEV
                     LVKNQKAATDYRAGKQNAFQFLVGQTLAAAQGRANPDLLKKILLEKLAEVRE"
     gene            complement(2221..3111)
                     /gene="miaA"
                     /locus_tag="PKK37_03175"
     CDS             complement(2221..3111)
                     /gene="miaA"
                     /locus_tag="PKK37_03175"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNR81412.1"
                     /translation="MKNKLIVIVGPNASGKSEIAVMIAKKFNGEVVSADSRQVYKGMD
                     IGSGKIKKSEMKGVPHHLLDVASPKSVFTVTRYRQLALKAVEKIRRAGKIPTLCGGTG
                     FYIQAVIDGITIPQVKPDWKLRQRLEKLPAKKLYSILRKLDPARAKSIEAKNPRRLIR
                     AIEIVKTTGKPVPALKKQPLDYEILFLGIKKNEKELKRLIERRADARIRKGMVAEVRK
                     LKNSGLSWNKLESFGLEYRAVSQFLQNKINRQKMVDTIKKEDWQYAKRQMTWFGRDKR
                     VNWIKTKSQAVKLAQKYLSK"
     gene            complement(3101..>3508)
                     /locus_tag="PKK37_03180"
     CDS             complement(3101..>3508)
                     /locus_tag="PKK37_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNR81413.1"
                     /translation="GFCGETEKQFQNTARIFKEIKYDMAYISRYSKRPGTAAALLDDN
                     VSDREKKRREEILNDIVKITALAHNKKWCGKTVDVLVDRVKNGFGFGKDRHYKTVKFR
                     IEKNDIKPGDIVNAKIIKAQSFGLMGALVKNEK"
BASE COUNT          907 a          771 c          907 g          923 t
ORIGIN      
        1 cccgccaaag tggtttgtct ggtggacgca ccgattggcg acagcacgca cgaagaacga
       61 atgcaaattt gtttgacggc gaaaaataac ggcgccgatg agctggatgt ggtggcgtca
      121 ctgcgcgata tcaagcatga aagatgggag caggtgtata aagatttggc cgaaatttgc
      181 aaaatcttgc cgaccaaagt gattatcggt tcgggttatt tgacggacaa ggagatcgct
      241 atggtgtcgc ggctggtcaa gcaggcgggc gcaatttgcg tgaagaccgc cacctcaaaa
      301 gatcccctgg aagagcgcga attaaaagaa aaggcctgcc atttggaaat tatgaaaaaa
      361 aacgcgccgg gattgttgat caaagcttcg ggaaacgtca agaccgccaa ggacgcgcgg
      421 gcgtatgtta aagcgggagc cgacattatc ggcacatcaa acggcgtggc cattgcacga
      481 ggataaataa gagttaatgt aaaattaaaa aatcaaaatg aaaaatgaca atgataaaat
      541 tcaaaattat caaacgttaa aatgaagcga tagcggaatt ttaactcctt aattttgaat
      601 tttgattttt ttattttaaa tttttatgga atacgttccc acaatcgggc tggagattca
      661 ctgcgaattg aaaaccgcgt caaagatgtt ttgttcgtgc cccaaccgtc cggataccgg
      721 tgcgcccaat accaatgttt gcccggtttg tttggggcat ccgggaaccc tgccggtggc
      781 gaatcgcgaa gcgatccgct tgacgcggct ggtcggtttg gcgctggatt cccggatttt
      841 ggaacattcc aaatttgacc gcaagaatta tttctatccg gatttaccca aagggtatca
      901 aatttcgcaa tacgatcagc cgctttgttc gggcggatat ttggagatta gcgacggcgg
      961 aaaacccaaa aaaatcggta ttacccgcgt tcatttggaa gaagacaccg gaaaattaat
     1021 gcattccgac gataaagacc attcgctggt ggattttaac cgcggcggcg tgccattgat
     1081 ggaactggtt accgaaccgg acattgagaa cgccgatcag gcgcgcgagt ttgtcaagga
     1141 actgcagttg attttgcgcc atttgggcgc atcggatgcg gatatggaaa aagggcagtt
     1201 gcgggtggaa gtcaatatca gtctgggccg gattgctccg gacggcaaaa aaaagctggg
     1261 cacgaaggtg gagatcaaga atttaaattc tctcaaagcg gtggagggca gtgtggcctt
     1321 tgaaatcggc cgccagacgg cgcggattaa gaatggggaa aaggtggtcc aggaaacgcg
     1381 gggatggaac gaattcaccg gccaaacttt ttctcagcgc gagaaagagg aggcgtttga
     1441 ttatcgctat tttcccgaac ccgacattcc gccgctggca atttcgcccg gagagatcga
     1501 aaagctaaaa gcggcggtgg gggaattgcc ggcaaaaaaa agatttcgcc tggccgaaca
     1561 atacggcctt ggcgcggcta tcgttgaaat tttcgtcgtt aaccgcgatt tgggcgaata
     1621 ttttgaaaaa atcgccagcg agctggaaga gtgggtcaat gtcaaagaag gcgattgcga
     1681 aaacagcgac gtccaaaacc atcagctggc gcggctttgt gccaactatc tcaccaccga
     1741 tgttttgggg gcgctggcgg gaaaatcgtt ttgcgaaaaa gaattcccgg tgtctccgga
     1801 aaattttgcc gaatttatct gtttaattaa caagggcgag attacttcca aaatcgccaa
     1861 acaagtgctg gcggaaatga tgaaaaccgg caatgatccc agcgcgatta tcacccaaaa
     1921 aggattgagt cagattcgcg acgaaaaaca aatcgccgcg atgattgacg aggtgctggt
     1981 caaaaatcaa aaagccgcga ccgattatcg cgccggcaaa caaaacgcgt ttcaattttt
     2041 ggtggggcaa acgctggcgg ccgcgcaagg ccgcgccaat ccggatttgt taaaaaagat
     2101 cctcctggaa aaattagccg aagtaaggga atagcgagtg taggggcagg tcataaagcc
     2161 gcgcccctac tttttttgat acgtaaaatt ggtcaaggtt tcaccttgac caattttgaa
     2221 ttatttggag aggtattttt gggctagttt aacggcttga gatttggttt ttatccagtt
     2281 aactcttttg tcgcgcccaa accaggtcat ctgccgcttg gcatattgcc aatcctcttt
     2341 ttttattgtg tcaaccattt tttggcggtt gattttgttt tgcaaaaatt gcgaaaccgc
     2401 acgatattcc agtccgaaag attccaattt attccaagac aggccggaat ttttgagctt
     2461 tctgacttcg gcgaccatgc cttttctgat tcgcgcgtcg gcgcgccgtt caatgagccg
     2521 ctttaattcc ttttcgtttt ttttgattcc cagaaacaaa atttcataat caagcggttg
     2581 ttttttcaac gccggcacgg gtttgccggt ggttttgacg atctcaatgg cgcggatgag
     2641 ccgccgcgga tttttggctt caatgctttt ggcgcgggcc gggtcaagtt tgcgcaatat
     2701 ggaatataat tttttcgccg gtagtttttc caatcgctgg cgcaatttcc aatccggctt
     2761 gacctgcgga atggtgatgc cgtcaatcac cgcctgaatg taaaaaccgg tgccgccgca
     2821 caaggtcgga atttttccgg cccgccggat tttttcaacg gctttcaatg ccagttggcg
     2881 gtagcgagtg acggtgaaga ccgatttcgg gctggcaacg tccaaaaggt gatggggaac
     2941 gcccttcatt tccgattttt tgattttgcc cgaaccgatg tccattccct tgtagacttg
     3001 gcgcgaatcc gcggaaacca cttcgccgtt gaatttttta gcgatcatta cggcgatttc
     3061 cgattttccc gaagcattcg gtccgacgat gacaatcagt ttatttttca tttttaacta
     3121 gtgcgcccat aagtccgaat gactgcgctt tgattatttt ggcgttaacg atgtcacccg
     3181 gcttaatgtc gtttttttcg attcggaatt tcacggtttt gtagtggcgg tctttgccga
     3241 agccaaaacc gttttttacg cgatcaacca aaacatcaac ggttttgccg caccattttt
     3301 tattatgggc gagcgcggtg atttttacaa tatcgtttaa aatttcttcg cgccgtttct
     3361 tttcccgatc gcttacatta tcatccagaa gtgccgcggc agtgcccggg cgcttggaat
     3421 agcgggaaat ataggccata tcatatttta tttctttgaa aatccgggcg gtattttgaa
     3481 attgcttttc cgtttcgccg caaaagcc
//
LOCUS       DAOQWE010000082         3484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_28160_length_3484_cov_4.446486, whole genome shotgun sequence.
ACCESSION   DAOQWE010000082 DAOQWE010000000
VERSION     DAOQWE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28160_length_3484_cov_4.446486"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2255
                     /locus_tag="PKK37_03185"
     CDS             <1..2255
                     /locus_tag="PKK37_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81414.1"
                     /translation="RCVECSIDPGVPQGCANGEACPSGTVCSNTVCCPAGTCGWGEGQ
                     WFTRHCAKNGDYRENRWARLVCRNGVWKTELNGWGCNAFTCDQGTCQDNLYCAYSLPL
                     NPSSQMHETGAYCAGTCKSCSAQGKNCGIVSDQCGGYIYCGACASGQTCNASGQCVSS
                     CTPATCLSLGKLCGVWNDNCGGTVNCGSCLADEFCNASGQCVASCVPATCSSLGKLCG
                     VWNDNCGGTVNCGSCLAGQSCNISGQCVADDSGDVDCTPNFSKKCADGHLYWYDSCGA
                     KEGMAQNCGTDSATQNFQCRGNWVQQEIITRDCANGACTENKSWSDSVNCALSGKICA
                     AGVCVANDITPPSLFGLSPSGTIYSANVALTVSTNEVSECRYGLQDMSFTSMALKFGS
                     SNKTYHSASIILPSPGNYNYFVRCADTAGNAATASAKISFVYALPAQSPAVTETPPSS
                     PVLPPVSDKTPPVISDALPAGDVFEGKVQISVTTDEKSSCKYDIAATDYDSMENFLES
                     DGTGTFHSKEISISAAGNYAYYVNCKDDAGNEAGQSTAIKFNYVQGESGPAISEIAPE
                     GAVYQGEIMLTAVTDVPAVCRYSGEDREFEEMTDLFSASDDGLRQSAMITLGDYGNYN
                     YHIRCRDEKGNTQGSYAAVSFEYKDPAAPVSGEAPVSLECYQVADGDRDGECDHTLDC
                     VCDPDCPVDGEDADPDCANVAVQESGTNRALFIGIGLLLLLVLIVIFAIIIMHRKKSG
                     QENQDENEFA"
     gene            2440..>3484
                     /locus_tag="PKK37_03190"
     CDS             2440..>3484
                     /locus_tag="PKK37_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81415.1"
                     /translation="MNKKLLLVFFSVFVFGLCRIHSSVDAAGEAGYWGFDENGGYVAS
                     DTSGNFNHGVLTNDVSWTPGKSGSALSFGGSGYVSIPMTSGLQPSGAVTVAAWIKVSG
                     IQDYYAIVSSYGSPNPPGASCNGGTAYTIRFSPDGKPSFGVTTGNNNLCAEDYVLSPD
                     NISFDAWHHIAGVYNGSQIVIYVDGAPKGYFSKTGTLIPQDSLEIGASKVYGQQYNFM
                     RGIIDEAKVYTRALSASEISQLASQFSCTPNCSGKVCGSDACGGSCGTCASGQTCSEA
                     GQCVAQGVCTPGEVSVCKVCKSDGLGWADDGSKCATGQKCSLGQCVSVPSDSDCTAKT
                     CSSAGYECGAWNDA"
BASE COUNT          795 a          874 c          955 g          860 t
ORIGIN      
        1 accgatgcgt tgagtgctct attgatccgg gggtgcccca agggtgcgcg aacggtgagg
       61 cttgcccgtc gggaacggtt tgcagtaata ctgtctgctg tcccgctggc acctgcggct
      121 ggggcgaggg ccagtggttt acacgtcatt gtgcaaaaaa cggcgattac cgcgaaaatc
      181 gctgggcgcg tttggtctgc cgcaacggag tttggaaaac tgagttaaac ggctggggat
      241 gcaatgcatt tacttgcgat caaggaacct gccaagacaa tctttattgc gcttattctc
      301 tgccgctcaa tccttcttcg caaatgcatg aaaccggagc ctattgtgcc gggacttgca
      361 aaagttgttc ggcgcaggga aaaaactgtg gtattgtatc tgaccaatgc ggtggctaca
      421 tttattgcgg cgcctgcgct tcggggcaaa cctgcaatgc ttccggccag tgcgtttctt
      481 cgtgcacgcc ggcaacatgt ttgtcattgg gcaaactatg cggtgtctgg aacgacaatt
      541 gcggcggcac ggttaactgc ggttcttgct tggccgatga gttttgcaac gcctccggcc
      601 agtgtgttgc ttcgtgcgtg ccggcaacgt gttcgtcgct gggcaaacta tgcggtgtct
      661 ggaacgataa ttgcggcggc acggtaaatt gcggttcttg cttggccggc caatcttgca
      721 atatttccgg tcagtgcgtc gccgatgatt ccggcgatgt cgattgcacg ccgaattttt
      781 ccaaaaagtg cgccgacggc catttgtatt ggtacgattc ttgcggcgcc aaggagggga
      841 tggcgcaaaa ttgcggcacc gattccgcga cgcaaaattt ccagtgccgc ggcaattggg
      901 ttcagcaaga gataatcacc cgggattgcg ccaacggcgc gtgcacggaa aacaaaagct
      961 ggagcgacag cgtcaactgt gcgctttccg gcaaaatttg cgccgccggc gtctgtgttg
     1021 ccaatgatat tacgccgccg tcgcttttcg gcctgtctcc ctcggggacg atttacagtg
     1081 cgaatgtggc gctcaccgtt tccaccaatg aggtttccga atgccgttac ggccttcagg
     1141 atatgtcgtt cacgtcaatg gccttgaaat tcggcagttc aaacaaaact taccattccg
     1201 cgtcaataat cctgccctct ccgggaaact acaattattt cgtccggtgt gccgataccg
     1261 ccggcaacgc cgccacggcc tccgccaaaa tatccttcgt ctatgccctg ccggcccagt
     1321 ctcccgcagt cacggaaacg cctccgagtt ccccggtctt gccgccggtt tcggataaaa
     1381 cgccgccggt gatttccgat gcccttcccg ccggcgatgt ttttgaggga aaagtgcaaa
     1441 tctcggtaac gaccgatgaa aaatcgtcgt gcaaatacga tattgccgcc acggattacg
     1501 attccatgga aaattttctg gaaagcgacg gcacgggcac tttccatagt aaagaaatat
     1561 caatttccgc ggcgggcaac tatgcctatt atgtcaattg caaagacgat gcgggcaacg
     1621 aggccggaca atcaaccgca ataaaattca actatgtcca gggcgaatcc gggccggcaa
     1681 tttcggagat cgccccggaa ggcgcggtct atcaggggga aattatgctc acggccgtta
     1741 ccgatgttcc cgcggtctgc cgttattccg gggaagaccg cgaatttgaa gaaatgaccg
     1801 acttgttttc cgcaagcgac gacggcttgc gccaatcggc gatgattaca ttaggcgatt
     1861 acggaaatta caattatcac atccgttgcc gggacgaaaa aggcaacacc caagggagtt
     1921 acgccgcggt ttcctttgaa tataaggacc cggccgctcc cgtttccggc gaagcgccgg
     1981 tgtcgcttga gtgctaccaa gttgcggacg gcgatcggga cggcgaatgc gaccatacct
     2041 tggactgcgt ttgcgatccg gattgcccgg ttgacggcga ggatgccgac cctgattgcg
     2101 ccaatgttgc ggttcaagaa tcggggacca accgggcgct gttcattggc atcggcttgt
     2161 tattgttgtt ggttttaatt gttatttttg ctattattat aatgcaccgg aaaaagtccg
     2221 gccaagaaaa tcaagacgaa aacgaatttg cttgaaacaa aatcgggctt ccaatggcga
     2281 ttcatttaaa ataagcgaaa aaatttaatc gttatgccat tcttgcgata aatataaaaa
     2341 taagaacatt gaatagttga ttttgccaag tattttaaac ttggcaaatt cgcgaaaaca
     2401 ataaaattcg gtaattatcc aacgttctaa aaaataaaaa tgaataaaaa attattgctg
     2461 gtgttttttt cagtttttgt ttttggatta tgccggatcc attcttctgt tgatgcggcc
     2521 ggcgaagccg gttattgggg ttttgatgaa aacggcggct atgttgccag cgacacctcg
     2581 ggcaatttca atcacggcgt gttgacgaat gatgtgtcat ggacgccggg aaaatccgga
     2641 tccgcgctgt ctttcggcgg tagcggatat gtgagcattc caatgacttc cggtttacag
     2701 ccgtccggcg cggttacggt cgcggcctgg atcaaggtgt ccggcatcca agattattac
     2761 gcgattgtca gctcttacgg ttcgcccaat ccgcccggcg ccagttgcaa tggcgggacg
     2821 gcttacacca tacggttttc ccccgacggc aaaccgtcgt ttggcgtaac caccggcaac
     2881 aacaatcttt gcgccgaaga ttatgttttg agccccgaca atataagttt tgatgcctgg
     2941 catcatattg ccggagtgta taacggatcg caaattgtta tctatgttga cggcgcgccc
     3001 aagggttatt tttcaaaaac cggcacgctc attccccaag attctttgga gattggcgcc
     3061 agcaaggtct acggccagca atataatttt atgaggggta ttattgatga agcgaaagtc
     3121 tacacccggg cgttatccgc atcggaaatc agccagttgg caagtcaatt cagttgcacg
     3181 cccaattgtt ccggcaaggt ctgcggttcc gatgcctgcg gcggttcctg cggtacttgc
     3241 gcttcggggc aaacctgcag tgaagcgggg caatgcgtgg cgcagggggt gtgcacgccc
     3301 ggcgaagttt ccgtctgtaa agtttgcaaa agcgacgggt tgggatgggc cgatgacggt
     3361 tcaaaatgcg ctaccggaca aaaatgttcg ctgggccagt gcgtttccgt tccttcggat
     3421 tccgattgta ccgccaaaac ctgttcttcg gccggatatg aatgcggcgc gtggaatgac
     3481 gcct
//
LOCUS       DAOQWE010000083         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_28334_length_3469_cov_7.182484, whole genome shotgun sequence.
ACCESSION   DAOQWE010000083 DAOQWE010000000
VERSION     DAOQWE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28334_length_3469_cov_7.182484"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..872
                     /locus_tag="PKK37_03195"
     CDS             144..872
                     /locus_tag="PKK37_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81416.1"
                     /translation="MRIKTKIVSLAAATFAGLLLGGNAFAGGAEQNADYQTISAQIQE
                     VQKKVSQLMTETQSGEKAAPVVNMELTPEKLDNIEREVTRIAKETERLKIEVAIYLTL
                     EDIRRKTMELATQITAGLNVSASMIAAAPAAPIVAALPADLPASKPQANEEIEAQIAI
                     VRRQIDELTEEMVAQTAAEKEIPAEERTVAGTEDEGAKGNVAASEEKDASLTVEKDGG
                     QDSAATKGGLWQAVGDFFKKLFAF"
     gene            999..1958
                     /locus_tag="PKK37_03200"
     CDS             999..1958
                     /locus_tag="PKK37_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNR81417.1"
                     /translation="MDKIGFSKLHMKNLKNNLRQFFLPCEENGGRARIFSGNFLLFFL
                     IGIAVLKIGFALFLHSFSGNPFYADITKTALIDLANYERSRSNLPPLAENPVLNQAAY
                     MKALDMAQGGYFNHVSPSGVNPWHWFSKAGYNYRYAGENLAIGFLDSGEVQKAWTASP
                     THHANIINNKYQEIGIAVLKANFQGNPATIVVQMFGARSGRKEAAPAGTALSQAPTAP
                     EESEVAARGVLGAQTAAPDQNSAGFKIAQFLARDFYFVMQWTVYLSLVAVILLLLVNF
                     TLKADLAHGDLLAKAVGFIAVMIVFALLDYGLIAALVPRNFVV"
     gene            1973..2692
                     /locus_tag="PKK37_03205"
     CDS             1973..2692
                     /locus_tag="PKK37_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNR81418.1"
                     /translation="MANAKKRKKTRRRFWNSPFLPLALLLAFLAFIALFWGNISWVVN
                     ANIWRQILSDAFPQIFPRPYVLVVKNPTLISGTPEVDNKPAIKSPGGGNSPASAQKPR
                     RDMLTIPGINITAPIITAKTGDAEVISGLLDSGVVLYPGSAPFGGSGQTVILGHSAPA
                     GWPKIKYEWVFSKINELEKGDMVVVTYDNITRYYKVAGSQIVNPKDGVPAPTVEGNSL
                     MLISCWPPGKDTKRIAVEAEL"
     gene            2902..>3469
                     /locus_tag="PKK37_03210"
     CDS             2902..>3469
                     /locus_tag="PKK37_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81419.1"
                     /translation="MNKILIVFFGFAVFLATAFVVVPVNAEYCNQGITAKLVNLGGGG
                     KPATVSITNTADCAAQATAASYKVYRQPYVESGWLDTQTLLDYETVILSPGETRTISL
                     DTADCMTQVDVFRGGVVYKLYDYLYDYYTTIVPLGELTHDPVCAMCQNQCVLNAHECS
                     GSSGRTCVQSGQCTAWGTWQTCDSRCYSC"
BASE COUNT          874 a          785 c          931 g          879 t
ORIGIN      
        1 ggtattgcat aaatcgggta aaatctgcta tcatctatta acgccgcggc cggagttttt
       61 tgcggtcgcg ggcgaggtcg acaaattcag caatgccaag cgcaatgcca ataaagttga
      121 ggacttttaa agtaaaagaa aatatgcgaa tcaaaaccaa gattgtgtcg ctggccgcgg
      181 caacttttgc cggtttgctc ttgggaggaa atgcttttgc cggcggcgcg gagcaaaatg
      241 ccgattatca aacgatatcg gcgcaaatcc aggaagtgca gaaaaaggtt tcgcaattga
      301 tgacggaaac gcagagcggg gaaaaggccg ctccggtcgt gaacatggaa ttgactccgg
      361 aaaaacttga taatattgaa agggaagtga cgcgaatcgc caaagaaacc gagcgattga
      421 aaatcgaagt cgcgatatat ctcacgctgg aagatattcg ccgaaaaacc atggagttgg
      481 ctacgcagat caccgccggt ttaaatgttt ccgcgtcaat gattgcggcg gcgccggccg
      541 cgccgattgt tgcggccttg cccgcggatt tgccggcctc aaaaccgcaa gcaaacgaag
      601 agattgaggc gcaaatcgcg attgttcgcc gccagattga tgagttaacc gaagaaatgg
      661 tggcgcaaac cgcggcggag aaggagattc cggccgaaga aagaaccgtg gccgggaccg
      721 aagatgaagg cgcaaagggg aatgttgcgg cctccgaaga aaaagacgct tctttgaccg
      781 tggaaaaaga cggcggccaa gatagcgcgg caacgaaggg aggcttgtgg caagccgtgg
      841 gagatttttt taaaaaactt tttgctttct aattttgatt gccggggcgc cggtgcctgt
      901 ccggcggcaa acaattgaac gcggcggcag accgcgtttt gttgtcttca aacgccataa
      961 aggttataat agacagtgat aagtccgacg atttaatttt ggataagatt ggattttcca
     1021 aattacatat gaaaaacctc aaaaataatt tgcggcagtt ttttttgccg tgcgaagaaa
     1081 atggcggccg cgcccggatt ttttcaggta attttttatt gttttttttg atcggtatcg
     1141 cggttttgaa aatcggattt gcgctgtttc tgcattcgtt ttcgggaaat cctttttacg
     1201 ctgacattac caaaaccgcg ctgattgatc tggcgaatta tgaacgttcc cgaagcaatt
     1261 tgccgccgct ggcggaaaat ccggtcttga atcaggcggc atatatgaag gcgctggata
     1321 tggcgcaggg cggctatttc aaccacgtga gcccttcggg ggttaatcct tggcactggt
     1381 tttccaaggc cggatataat tatcgttatg ccggtgaaaa tttagccatc ggatttttgg
     1441 attccgggga ggttcaaaaa gcgtggactg cgtcgccgac ccatcatgcc aacattatta
     1501 acaacaaata tcaggagatc ggtattgcgg ttttaaaagc taactttcag ggaaaccccg
     1561 ccacgatcgt ggtgcagatg ttcggcgccc ggtccggccg caaggaagcc gcgccggcgg
     1621 gcaccgcatt aagccaggcg ccaacggcac cggaggaaag cgaagtagcc gcccgcggcg
     1681 ttttgggcgc gcagaccgcc gcgccggatc agaattcggc gggcttcaag attgcgcaat
     1741 ttttggcgcg ggatttttat tttgtaatgc agtggacggt ttatttgtct ttggtcgcgg
     1801 tgatattgct tttgttggtc aatttcactc ttaaagcgga tttggcgcac ggagatcttt
     1861 tggcgaaagc cgtcggattt atcgccgtga tgatcgtttt cgcgcttctg gactacgggt
     1921 tgattgcggc cctggttccc cgaaattttg ttgtttaatc cctccttgtc ccatggcgaa
     1981 tgcaaaaaag agaaaaaaaa ctcgccggag gttttggaac agtccgtttt taccgctggc
     2041 gcttttgttg gcgtttttgg cgtttatcgc gctgttttgg ggcaacattt cctgggtcgt
     2101 gaacgccaat atctggcggc aaattctctc ggatgcgttc ccgcaaattt ttccgcggcc
     2161 gtatgttttg gtcgtcaaga atcctacttt gatttccggc acgccggaag ttgacaataa
     2221 accggcgatc aagtcgccgg gggggggaaa ttctcctgct tcggcgcaaa aaccccggcg
     2281 cgatatgctc accattcccg gaatcaacat taccgcgccg attattaccg ccaagaccgg
     2341 cgacgcggaa gttatttccg gcttgctgga ttcgggtgtc gtgctctatc cgggttcggc
     2401 gccattcggc gggagcggac aaaccgtcat cctgggacac agcgcgccgg ccggctggcc
     2461 gaaaatcaaa tatgaatggg tgttttccaa gattaacgag ctggaaaaag gcgatatggt
     2521 ggtggtaacc tatgacaaca tcactcgcta ttataaggtg gccgggtccc aaattgtgaa
     2581 tcctaaagac ggcgtgcccg caccaacggt ggaaggcaat tccctgatgc tcatcagttg
     2641 ctggccgccg ggcaaggaca ctaaacgtat cgccgtggaa gccgaattat aaaatatagg
     2701 cataaaatta aagcggtcca tccctcacta ctgcaagggt cagaccgctt taattttagg
     2761 cccagtgagt ggttaatgtg tcctaccttg acaaatttct cattttttgc tatactacaa
     2821 aacgaagagt aataataccc tattcttttt atcggaaatt tcaatagaat atcattaatt
     2881 aataaacaat cagaagaaaa tatgaacaaa atattaattg tattttttgg atttgcggtt
     2941 tttttggcaa cggcttttgt tgttgtgcct gttaatgccg aatactgcaa tcagggaatt
     3001 acggctaaat tggtcaatct tggaggcggg ggaaaaccgg caaccgtgtc cataacaaac
     3061 actgctgatt gcgccgccca agcaactgcg gcgtcctata aagtgtaccg gcaaccatac
     3121 gttgagtccg gttggcttga tacgcaaacg ttgcttgatt atgaaacggt gatattgagc
     3181 ccgggtgaaa ctcggacaat ttcccttgac accgcagatt gcatgaccca agttgatgta
     3241 tttcgtggcg gcgtggttta taaattatac gattatttat acgattatta caccacaatt
     3301 gttcctttgg gcgagcttac acacgatccg gtttgcgcaa tgtgccaaaa tcaatgcgtc
     3361 ttaaacgctc atgaatgttc cgggtcttcg ggaagaactt gcgtccagag cggccaatgc
     3421 accgcctggg gaacatggca aacctgcgat tcgcggtgct attcctgcg
//
LOCUS       DAOQWE010000084         3417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_28973_length_3417_cov_8.834622, whole genome shotgun sequence.
ACCESSION   DAOQWE010000084 DAOQWE010000000
VERSION     DAOQWE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28973_length_3417_cov_8.834622"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PKK37_03215"
     CDS             complement(<1..1262)
                     /locus_tag="PKK37_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNR81420.1"
                     /translation="MMIKDNIFRGYDIRGTYPDELNENAVYWTTRAFCELYPASRRVV
                     LARDPRPSSPALAEAARRAFLEAGRVVIDLGIAPDPLWYFAIMHYGYDGGLMISGSHN
                     PSHHNGLTYHGRAGAGAESEELMGESLQRLKRAAQALEVKNAAPGAPAGKLAESDPSR
                     EYIDYVASKIKFNRPLKIVLDTGNGACGYLPERLFKKLGCKVETMFGDFDGTFPNHMP
                     DPYVAANRAALEARVKETGADMGFIYDTDGDRVGIIDNRGRAANGDDTLLVLARQALA
                     QKTGPVVHCMRASKAFIDDVRTRGGEPMFSVSHHNAIRDNVKKHNAVFGGEITFHYAF
                     PLDYYLVDDAVFASAKLAQAASEQNDFAAYVDSLPRYFASPELNVDCADDVKFGVIEK
                     LQKYLRDNKYNFIDVDGARINFADGWALA"
     gene            1690..2235
                     /gene="rpsF"
                     /locus_tag="PKK37_03220"
     CDS             1690..2235
                     /gene="rpsF"
                     /locus_tag="PKK37_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNR81421.1"
                     /translation="MMKHYEYTYLTRQDMSEDDAKNLQDKIGSFVTGKGGAIVDAPKA
                     RKRRLAYAIKKQNAAYLNTILFSFDPQNLADLKKDIAGEVEIMRGLILAYDPKKMEKE
                     VRRGRPAIAAAEAPAETKPKSEKTSPKEKMPAEESEENKKDAPAEPAPAAAEAPAKPK
                     RRTKIKAELRDIEEKLDEILK"
     gene            2377..2883
                     /locus_tag="PKK37_03225"
     CDS             2377..2883
                     /locus_tag="PKK37_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNR81422.1"
                     /translation="MNLNKVFLIGRLTRDPEIRVMPSGQQVANFGMATDSFYTDKNSG
                     QRQQKTEFHNIVMFARLAEIAGQYLKKGSLIMIEGRLQTRDWTDQAGNKRYRTEIIAE
                     NMQMGPRQDGGGGQSSAENYAAPAAAKVSEDRPAVKSAKPAPAGSAEPEIPIIEEDGD
                     INVKDIPF"
     gene            3011..3196
                     /gene="rpsR"
                     /locus_tag="PKK37_03230"
     CDS             3011..3196
                     /gene="rpsR"
                     /locus_tag="PKK37_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNR81423.1"
                     /translation="MACYFCQKNIQTIDFKDTEILRHFTSGTGKIRPKKKTGACATHQ
                     RQIAQAVKRARFLALMP"
BASE COUNT          975 a          872 c          788 g          782 t
ORIGIN      
        1 gccaacgccc agccgtcggc aaaatttatc cgcgcgccgt caacatcaat gaaattatat
       61 ttattatcgc gcaaatattt ttgcaatttt tcaatcacgc cgaatttaac atcatcggca
      121 cagtcaacat tcagttccgg actggcaaaa tagcgcggca aagaatcaac ataggcggca
      181 aaatcattct gttcggacgc cgcctgcgcc aattttgccg aggcgaacac cgcgtcgtcc
      241 accagataat aatccaaggg aaacgcgtaa tggaaagtga tctcgccgcc gaaaaccgcg
      301 ttatgttttt taacattatc ccgaatcgca ttgtggtgcg acacggaaaa catcggctcg
      361 cctccccgag tgcgcacatc gtcaataaat gccttggaag cgcgcataca atgcaccacc
      421 ggcccggttt tttgcgccag cgcctggcgc gccagcacca gcaaagtgtc gtcgccgttt
      481 gccgcgcggc cgcgattgtc aatgatgccc acgcgatcgc cgtcggtatc atagataaac
      541 cccatatcgg ccccggtttc tttcactcgc gcttccagcg ccgcgcggtt ggcggcaaca
      601 taaggatcgg gcatatggtt gggaaaagtg ccgtcaaaat cgccaaacat cgtttcaacc
      661 ttgcatccca attttttaaa cagtctttcg ggcaaatagc cgcacgcgcc gttgccggtg
      721 tccaaaacaa ttttcagcgg ccggttgaat ttgatttttg acgcgacata atcaatgtat
      781 tcccgcgacg ggtcggattc ggcaagcttg cccgccggcg cgccgggagc ggcatttttt
      841 acttccaacg cttgcgccgc gcgctttagc ctctgcaacg actcgcccat cagctcttcg
      901 ctttccgcac cggcaccggc gcggccgtga taggtgaggc cattgtgatg ggacggattg
      961 tgcgatccgg agatcatcag tccgccgtca tatccgtagt gcataatggc aaaataccac
     1021 aacggatccg gcgcgatgcc caaatcaatc acaacacgcc cggcctccaa aaacgcccgg
     1081 cgggcggcct cggccagcgc cggtgaactg ggacggggat cccgcgccaa aaccacccgg
     1141 cgcgatgccg gataaagctc gcagaacgcc ctcgtggtcc aataaaccgc gttttcgttc
     1201 aattcgtcgg gataagtgcc gcgaatatca tatccgcgga aaatgttgtc tttaatcatc
     1261 atcaatcaat agacctactg cgtaaatatt tcctactgca atttccatat ttcggctacg
     1321 ccgaattcgt caatcgtcgg catatttaag ccaatatgcc tcctcactcc tcattcggct
     1381 cgcgcgaaat ctgaaaattt cgtcgcgtaa atatttacgc agtaggtcta atttttaatt
     1441 tataattttt aatttaaaaa caatttttaa tttaaaaatt ttaaaattga attttgttta
     1501 aaaatttcaa atttcaaatt aaaaattttc ttttattata tcacaagacc cgcctattcc
     1561 aatgattgcc caatgatgtc aaatgattga cgagagtcgt ttttaatgct aatatgcgcc
     1621 tataatccaa gccatctccc ggccgaaaaa tgcggacggg acccgttaac agatggtcgg
     1681 aaaataaata tgatgaagca ctacgaatac acttatctga cgcggcagga tatgtccgag
     1741 gacgacgcca aaaatttgca ggacaagatc ggatcgtttg tcaccggcaa gggcggcgca
     1801 atcgttgacg cgcccaaagc ccgcaaacgg cggctggcct acgcgatcaa aaagcaaaac
     1861 gccgcgtatt tgaacacgat tttgttttct tttgaccccc aaaatctggc ggatctcaaa
     1921 aaggacatcg ccggcgaagt tgaaattatg cggggattga tcttggctta cgatcccaag
     1981 aaaatggaaa aggaagtccg ccgcggccgc ccggcgattg ccgccgcgga agcgccggcc
     2041 gagacgaagc cgaaatcgga aaaaacttct ccgaaagaaa aaatgcccgc cgaagagagt
     2101 gaagaaaaca aaaaagacgc gccggcagaa cccgcgccgg ccgccgcgga agcgccggcc
     2161 aagccgaagc gccggacaaa gatcaaggcc gaattgcgcg acattgaaga aaagctggac
     2221 gaaatcctaa aataattcag caaaaccaaa tgtcaaaatc caaatgtcaa ataaaatcct
     2281 aaatccaaat gtcaaaagga aatacgaatt tcttttgaac tttggacttt gatcttaatt
     2341 tgacatttga gcttttgatt ttggatttaa attctaatga atttgaataa ggtattctta
     2401 atcggacggt tgacgcgcga tccggaaatc cgggtgatgc caagcggcca gcaagtggcc
     2461 aatttcggca tggcaaccga cagtttttac accgacaaga attccggcca gcgccagcaa
     2521 aaaacggaat ttcacaacat cgtgatgttc gcgcgattgg cggaaatcgc cggccagtat
     2581 ctgaaaaaag gatcgctgat aatgattgag ggccgcttgc agacgcgcga ctggaccgat
     2641 caggcgggca acaagcgcta ccgcaccgaa atcatcgccg aaaatatgca aatggggccg
     2701 cggcaagacg gcggcggcgg gcaatcatcg gccgaaaatt acgcggcgcc ggccgccgca
     2761 aaagtttcgg aggaccggcc ggcggtaaag tccgccaagc ccgctcccgc cggaagcgcc
     2821 gagccggaaa ttcccatcat tgaggaagac ggcgacatta acgtgaaaga catccccttc
     2881 taaaccaata aattcccaat tttcaatacc caatttccaa tgaattttca atgattcaat
     2941 gaccaaacaa aatgcggtat tatcgtttga aaattgaaaa ttgaaaattg aaaattgcga
     3001 agcgaatatt atggcttgct atttctgtca aaaaaacatc caaacgattg attttaagga
     3061 caccgagatt ctccggcatt tcacttccgg aaccggtaaa atccgcccca aaaagaaaac
     3121 cggcgcttgc gccacgcacc agcgccaaat cgcgcaagcg gtcaagcgcg cccgcttttt
     3181 ggcactgatg ccgtagggaa tcgcaaaaaa ttgataatca acaaaaaatc cgccggaagc
     3241 ggattttttg ttgattgatg aatgattatt ggcgattggc aatcaacaat aattgataat
     3301 tgataagtga taagtgataa gtgattattt attatttatt aatgatcaag gattactctt
     3361 tggaatcgtc aaaagtggcg tcaattttca ctttcagttc ggattttatt ttttcca
//
LOCUS       DAOQWE010000085         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_29096_length_3407_cov_7.986575, whole genome shotgun sequence.
ACCESSION   DAOQWE010000085 DAOQWE010000000
VERSION     DAOQWE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29096_length_3407_cov_7.986575"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PKK37_03235"
     CDS             complement(<1..740)
                     /locus_tag="PKK37_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNR81424.1"
                     /translation="MDFVHLHTHTHYSLLDGLAQIDPLLDKVQSLGMSACAITDHGNM
                     YGAVEFYKKAKKRGIKPIIGCEVYVALESRFSRRPQIDDARHHLVLLVKNEAGYRNLV
                     KLVTKAHLEGFYYKPRVDEELLAEHQEGLIAMTACIQGKIPRLAIAKKMDEALECARR
                     YQKMFGKENFFLEIQHHPNLPDQSPANEGLIEIARKTGIGLVATNDIHYLDADDMEAQ
                     DILMLINTGADINNPERLTMKGEDFSMR"
     gene            complement(921..1616)
                     /locus_tag="PKK37_03240"
     CDS             complement(921..1616)
                     /locus_tag="PKK37_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81425.1"
                     /translation="MEEFVIAGKPVLIWSKKDIDTEMEQASLDRRDANFAGKTLKSFS
                     LMEKDFDYGINFQNTLIIGNVFFADASVNGSVNLANAAIDGSFFFNRGKLIGDLLMEK
                     AWVGQTINLSGINITGSVLMPQTRINGFVSLAKAVALGNIDLRGAEVQNYLQGELKVN
                     GDVLCENAQINGFLDLSGATVAGDVNLKSALIRQRLAARDLKLEGNFSLKDCIYNKTL
                     VDLGGLPEEKIIR"
     gene            1775..2395
                     /locus_tag="PKK37_03245"
     CDS             1775..2395
                     /locus_tag="PKK37_03245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNR81426.1"
                     /translation="MIGVDEAGRGPLAGPVMAAAVAVVLPTNRCDFAGLLGTVRDSKE
                     MAAAAREAVYRRIICHKNLVCASSAVGEKIIDKINILEASKLAMRRAVKRAIAQMKDK
                     ISAGKIVCLIDGNFSIEIPYEQKSIIGGDAKIFCIAAASIAAKVRRDRLMEKIGKLYP
                     EYGFARHKGYGTKEHLAAIEKHGLCSVHRRTFAPCASAARKGIMKK"
     gene            2423..2965
                     /locus_tag="PKK37_03250"
     CDS             2423..2965
                     /locus_tag="PKK37_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81427.1"
                     /translation="MSPAKKRNKLDRAAAIGFAVNFCAMFSLIFTVGLLAVAGIVGGN
                     SSGAASLSQGMKQAVCSCPPAEECSVAPLAPAVLFPVSAQQPMETALEISVDEGGFSS
                     REINLSNSSSRTLKIVNKGVRVHSFAITETGMNSGAIEPGRFVTVALDEPPAGGGKAE
                     FYSAEPEDSGENFRGTIVFE"
     gene            3176..3394
                     /gene="rpmF"
                     /locus_tag="PKK37_03255"
     CDS             3176..3394
                     /gene="rpmF"
                     /locus_tag="PKK37_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNR81428.1"
                     /translation="MHLFLKNPSFSQCAKCGKPVKSHHACPFCGHYRGKEAVDVFSRL
                     DRKQRRDRESEIAAQGAAKETKTSETAG"
BASE COUNT          797 a          856 c          883 g          871 t
ORIGIN      
        1 cttctcattg aaaaatcttc gcctttcatc gtgaggcgtt caggattatt gatgtcggcg
       61 ccggtgttga tgagcatcaa aatatcctgc gcttccatat cgtcggcatc caaataatga
      121 atgtcgttgg tggcgaccaa cccgatgccg gtctttcggg cgatttcaat caatccttcg
      181 ttggccggcg attggtcggg aagattcgga tggtgctgaa tttccaaaaa gaaattttct
      241 ttgccgaaca ttttttgata gcggcgcgcg cattcaagcg cctcgtccat tttcttggcg
      301 atggccaggc gggggatttt tccctggatg caggcggtca tggcgatcaa tccctcttgg
      361 tgttcggcca gaagttcttc gtcaacacgc ggcttgtaat agaacccttc caaatgcgct
      421 ttggtgacca atttcaccaa attgcggtag cccgcttcat ttttcaccag cagaaccaga
      481 tggtggcgcg cgtcgtcaat ctgcggccgg cgcgaaaagc ggctttccag cgcgacatag
      541 acttcacagc cgatgatcgg cttgatgccg cgttttttgg cttttttata gaattccacg
      601 gcgccgtaca tattgccgtg gtcggtgatc gcgcaagcgc tcattcccag ggattggacc
      661 ttatccaaaa gcggatcgat ttgcgccaat ccatccagca aactgtaatg ggtgtgagtg
      721 tgtagatgga cgaaatccat ggtaaaaata caaaattaaa aaatcaaaat gaaaaattaa
      781 gttaatatgc tttgcgccaa ttatttttgc ccgacacgga tatttttctg ctgaaaaatt
      841 cctcgtgttc gcgccgtcgc gctcgcaggg tcgggcaaaa ataattggcg caaagcatgg
      901 attttggttt ggtaagcttc ctatcggatg attttttcct ccggcaggcc gccgagatca
      961 acgagtgttt tattataaat gcaatctttg agggaaaaat ttccttcaag ttttaaatcg
     1021 cgcgccgcca gccgctgtct tatgagcgcg cttttcagat tcacgtcgcc ggcgacggtg
     1081 gcgccgctca aatccagaaa accgttgatt tgcgcgttct cgcacagcac gtcgccgttg
     1141 actttcagtt ctccttgaag gtaattctgc acctccgcgc cgcgcagatc aatatttccc
     1201 agcgcgaccg ctttagccag gctcacaaac ccgttgattc ttgtttgcgg catcaaaacc
     1261 gatccggtga tgtttatccc ggacaaattt atcgtttgcc cgacccacgc cttttccatc
     1321 aacagatcgc cgatgagctt cccccgattg aaaaaaaacg agccgtctat cgccgcgttt
     1381 gcgaggttga cgctgccgtt aacgctggcg tccgcaaaaa acacgttgcc gataatcaaa
     1441 gtgttttgaa aattaattcc gtaatcaaaa tccttttcca tcaacgagaa agacttcaac
     1501 gtctttccgg cgaaattcgc gtcgcggcgg tcaagcgagg cctgttccat ttcggtgtca
     1561 atgtcttttt tgctccagat gagcaccggt ttcccggcga tgacaaattc ctccattgat
     1621 tttaccgcaa aagaattaag gttataatct acttggggcg ctctttggga aacgtccctg
     1681 ccgccattat aactgaaatc aacaaaaatt gaaatctgcc cgttgtgcca atttcgtttg
     1741 cgaatcccgt tgtttccggc gcggattcaa actggtgatc ggcgttgacg aagccggccg
     1801 cggcccgctg gccggaccgg tgatggccgc ggcggtagcc gtggttttac cgaccaaccg
     1861 gtgcgatttt gccggtctgc taggcactgt gcgcgactcc aaagaaatgg cggccgcggc
     1921 acgcgaagcg gtctatcgcc ggattatctg tcacaaaaac cttgtttgcg cgtcgtcggc
     1981 ggtcggggaa aaaatcattg acaagatcaa tattttggaa gcgtcaaaac tggcgatgcg
     2041 ccgtgccgtc aagagagcga ttgctcaaat gaaagataaa atttccgccg gcaaaatcgt
     2101 ttgtctgatt gacggcaatt tttccattga aattccttac gaacagaaat caatcattgg
     2161 cggcgatgcc aaaatttttt gcatcgccgc ggcgagcatt gccgccaaag tccggcgcga
     2221 ccgtctgatg gaaaagatcg gcaaacttta tcccgaatac ggatttgccc ggcacaaagg
     2281 ctatggcacc aaggagcatt tggcggcaat tgaaaagcac ggcctctgtt ccgtccatcg
     2341 ccgcaccttt gccccttgcg cttccgccgc ccgcaagggt ataatgaaaa aatgaaaggt
     2401 aataaggtaa taatataaaa aaatgagtcc tgcaaaaaaa cgcaacaagc ttgatcgcgc
     2461 ggcggccatc ggttttgccg tgaatttttg cgcgatgttt tccttgattt ttaccgtcgg
     2521 ccttttggcg gtggcgggca ttgtcggcgg caactcaagt ggcgcggcgt cgttgtcgca
     2581 aggaatgaaa caagccgtct gttcctgccc gccagcggag gaatgttcgg tggcgcctct
     2641 ggcgccggcg gtattgttcc cggtttccgc gcaacagccg atggaaaccg cgctggagat
     2701 tagcgttgac gaagggggat tctcctcgcg ggaaatcaat ctttcaaatt ccagttcccg
     2761 gacgctcaag atcgtcaata aaggcgtccg cgtccattct tttgcaataa cggaaactgg
     2821 gatgaattcc ggcgcgattg aaccggggcg gtttgtgacg gtggcgctgg acgagccgcc
     2881 cgccggcggc ggcaaggcgg aattttattc ggcggaaccc gaggatagcg gcgaaaattt
     2941 tcgcggcacg atcgtttttg aatgatagat aatgcacctc tcttcggcga ggcacgcgga
     3001 gaaaaatttg attttctgtg gaatttgtgg taaaaggcag tcgttgttta attttgagtc
     3061 cggttgattg aattctgatt ttttccatta ctcatcaact cattactcat taactatttt
     3121 tatgggtggc gtaccaaaaa aaagacatac caaaggaagc cggaaccagc gccggatgca
     3181 cttgtttctg aagaatcctt ctttctctca gtgcgcgaaa tgcggcaaac cggtcaaatc
     3241 ccatcatgcc tgcccgtttt gcggccatta tcgcggcaag gaggcggtgg atgtgttttc
     3301 ccgtcttgat cgcaaacagc ggcgcgaccg ggaatcggag atcgcggccc agggtgcggc
     3361 gaaagaaaca aaaacttccg aaacggccgg ttaggccggc ggttgtt
//
LOCUS       DAOQWE010000086         3355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_29769_length_3355_cov_9.800909, whole genome shotgun sequence.
ACCESSION   DAOQWE010000086 DAOQWE010000000
VERSION     DAOQWE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29769_length_3355_cov_9.800909"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1342
                     /locus_tag="PKK37_03260"
     CDS             83..1342
                     /locus_tag="PKK37_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81429.1"
                     /translation="MGYGHQRTAHVLRSLSGGEVINANDYPGIPPRDRKIWQESRRFY
                     EAISKFKRIPMVGHAVFAAYDRLFQRIVDFYPMKDLSRAPFLTNRTYGLIKKGWGRHF
                     VAQLEKDPRPLVTTFFITAFMAEEFNYSEEIYLVVCDADISRQWAAKNPISSRINYCA
                     PTPRVVERLKLYGVPKKRIFLTGYPLPEENIGDEGGILKEDLAQRLANLDPNNVYLSK
                     YCQLVNNSLCAPTLKTRHPLTIMFSAGGAGAQGEIGLALARQLKDRIVAGKLKLILSA
                     GINERVKYFYERGVLAAGLDAEETPGLEILFETTIAKYFDRFNKTLRATDILWTKPSE
                     LSFYAALGIPIVIAPPIGSQEDFNKSWLLKSGFGLEQEDLRCIGQWLDDWRENGYLAE
                     AAMEGFIEGERMGAANIRNLVFGESSN"
     gene            1460..2374
                     /locus_tag="PKK37_03265"
     CDS             1460..2374
                     /locus_tag="PKK37_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81430.1"
                     /translation="MAWLFVALAAFSLFAAMALIDKFLLSEKIGDPRIYAFYVGILSS
                     AALALLPFGFWSHPAPAVFAWAFLAGAAQIYGNFFYFSALKRMEAARAVPVVGSLAPI
                     AAFFLTAAVSGGAAVLSQKEMAGFFLLIAGGWMIMARGISIKKEAILFVLAAPLFFAA
                     NAVAAKLVYLQLPFLNGFLLMAFGSVAAAITFLASKSVRRTVFSFGRGTERAKGKPGG
                     LFFLGQALGASAFLLQSFAISLAPQASVPAINAMAGVQYGLIFVFSLVLARRWPAIFG
                     EKISFAGLAQKIAAILLIAAGLAVFASN"
     gene            2381..>3355
                     /locus_tag="PKK37_03270"
     CDS             2381..>3355
                     /locus_tag="PKK37_03270"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNR81431.1"
                     /translation="MRKKIVISGVLILLLAGALGFFCFWRSPEAENVVWGVSFSQKQA
                     AALGLDWQAAYSALLDDLDVKKIRIAAHWDLLAPRPGEYDFSDLDWQMDEAARAGAKT
                     VLAIGMKTPRWPECHLPEWAKTLDKERQQAAVLALLEAAAIRYRDHPALESWQVENEP
                     LFPFGECPWRDEKFLQKEIELVKSLDGNKRPVVISDSGEGSWWLNAARAGDIAGITMY
                     RRVYFSELGIYVDYLLPPAFYAFKAELVGLLFKKKVICVELQAEPWAIGQKYDGGAND
                     AETMDIGRFRKNVAFARSTGLDEFYLWGGEWWYWMKTKKNRPEFWEEAK"
BASE COUNT          715 a          767 c         1024 g          849 t
ORIGIN      
        1 aaaaagattt tctttgaaga tgaaaacgga aacggaaacc aaaaatcgcc gcgcgcggaa
       61 ggcgtgggtg gtgggcgtga atatgggtta tggccaccag cgcaccgcgc acgtgctccg
      121 gtcgctgtcc ggcggcgaag tgatcaacgc caacgattac ccgggaattc cgccgcgcga
      181 ccggaaaatc tggcaggaaa gccgccgctt ttacgaagcg atctcaaagt tcaaacgcat
      241 tccgatggtc ggccatgcgg tgtttgcggc ctatgaccgg ctttttcagc ggatcgttga
      301 tttctatccg atgaaagatt tgtcgcgggc gccgtttttg acgaaccgca cttacggact
      361 aatcaaaaaa ggctggggaa ggcatttcgt ggcgcagttg gaaaaagacc cgcggccgct
      421 ggtgaccact tttttcatca cggcgtttat ggccgaagaa ttcaattatt ccgaagaaat
      481 ttatctggtg gtttgcgatg ccgatatcag ccgccaatgg gcggccaaaa atccgatttc
      541 cagccgcatc aattattgcg cgcccacgcc gcgcgtggtg gaacggttaa agctctacgg
      601 ggtgcccaaa aaaaggattt ttctgaccgg ctatcctttg cccgaggaaa acatcgggga
      661 cgagggcggt attcttaaag aagatttggc gcaacggctg gcgaatttgg atcccaacaa
      721 tgtctatctt tcaaaatatt gccagctggt gaacaattct ttgtgtgcgc cgactttaaa
      781 aacgcggcat ccgctcacaa taatgttttc cgccggcggc gccggcgcgc agggcgaaat
      841 cggattggcg ctggcgcgcc agctgaaaga ccggattgtt gccggcaaat tgaaattgat
      901 tttgtcggcg ggcattaacg aaagagtcaa gtatttttat gagcggggcg tgctggccgc
      961 gggactggat gcggaggaaa ccccgggatt ggaaattctg tttgaaacga cgatcgcgaa
     1021 atattttgac cgcttcaaca aaacgttgcg cgccaccgac attctttgga ctaagcccag
     1081 cgaactttcg ttctatgccg cactgggaat tcccattgtg atcgcgccgc ccatcggttc
     1141 gcaggaagat ttcaacaaaa gctggctgtt gaaatccggg ttcggtttgg agcaggaaga
     1201 cttgcgctgt atcggccagt ggcttgacga ctggcgggaa aacggttatc tggccgaggc
     1261 ggcgatggaa ggcttcattg aaggggagag gatgggcgcg gctaatatcc gaaaccttgt
     1321 tttcggcgaa agctctaatt gaaaatgtaa aaatcaaaat gtaaaattaa ggtaaaattc
     1381 ctgacggaat tttgatttaa tattttttaa ttttacattg tcatttttca ttttgatttt
     1441 taaattttaa atttgaaaca tggcgtggct tttcgtggcg ctggccgctt tttctttgtt
     1501 cgcggcgatg gcgctgattg acaaattctt gctttccgaa aaaatcggcg atccgcggat
     1561 atacgctttt tatgtgggaa ttttgagttc ggcggcgctg gcgcttttgc cgttcggatt
     1621 ttggagccat cccgccccgg cggttttcgc gtgggcgttt ttggccggcg cggcgcaaat
     1681 ttacggcaat tttttctatt tttcggcgct caagcggatg gaagccgccc gcgcggtgcc
     1741 cgtggtcggc agtctggcgc cgattgccgc gtttttcctg acggccgcgg tttccggcgg
     1801 cgcggcggtt ttgagccaaa aggaaatggc ggggtttttt ttgctgatcg cgggcggctg
     1861 gatgataatg gcgcgcggaa tttcaattaa gaaagaagcg attttgtttg ttctggccgc
     1921 gccgctattt tttgccgcca atgccgtggc ggcaaagctg gtttatttgc agttgccttt
     1981 tttgaacgga tttttgttga tggcgttcgg gtccgttgcc gcggcgatca cttttttggc
     2041 gtcaaaatcg gtgcgccgga cggtgttttc gttcgggcgc ggaaccgagc gcgcgaaagg
     2101 gaaacccggg ggtttgtttt tcctggggca ggccttgggc gcgagcgcgt ttttactgca
     2161 aagctttgcg atatcgctgg cgccgcaggc aagcgtgccg gcgatcaacg ccatggccgg
     2221 cgtgcaatac gggttgattt tcgtcttttc gctggttctg gcgcgccgct ggccggcaat
     2281 tttcggagag aaaatttctt ttgccggcct ggcgcaaaag attgccgcaa ttttgctgat
     2341 tgccgccggc ctggccgttt ttgcgtcaaa ttaaatgact atgcgaaaga aaattgtcat
     2401 ttcgggggta ttaattttat tgctggcggg cgcgttgggg tttttctgtt tctggcggtc
     2461 gccggaagcg gagaatgtcg tttggggcgt ttcgttctcg caaaaacaag ccgcggcgtt
     2521 ggggcttgac tggcaagccg cgtattcggc gctgttggat gatctggatg tcaaaaaaat
     2581 tcggatcgcc gcgcactggg atttgctggc gccccgtccg ggggagtatg atttttccga
     2641 tctggattgg caaatggacg aggcggcgcg cgcgggcgcc aaaacggtgc tggcaatcgg
     2701 gatgaaaaca ccgcgctggc ccgagtgcca tcttccggaa tgggcgaaga ctctggacaa
     2761 agaacgccag caagcggcgg ttttggcact gctggaagcc gcggcgatcc gctatcgcga
     2821 ccatccggcg ttggagtctt ggcaggtgga gaacgaaccg ctgtttcctt ttggcgaatg
     2881 tccttggcgg gacgaaaaat tcctgcaaaa ggagattgag ctggtcaaat cccttgacgg
     2941 aaacaaaagg cctgttgtaa tatcggatag cggggaggga tcgtggtggc ttaacgccgc
     3001 gcgtgccggc gatatcgcgg gcatcacaat gtaccgccgg gtttattttt ccgaattggg
     3061 gatctatgtt gattatcttc tgccgccggc gttttacgcg tttaaggcgg aattggtcgg
     3121 cttgttattc aaaaagaagg tgatttgcgt tgaattgcag gccgagccgt gggcgatcgg
     3181 ccaaaaatac gatggcggcg ccaatgatgc cgaaacgatg gacatcggcc ggtttcgcaa
     3241 gaatgtcgcg tttgcccgga gcaccggact ggatgaattt tatctgtggg gcggcgaatg
     3301 gtggtattgg atgaaaacaa aaaagaaccg cccggaattt tgggaagaag ccaaa
//
LOCUS       DAOQWE010000087         3279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_30811_length_3279_cov_2.561104, whole genome shotgun sequence.
ACCESSION   DAOQWE010000087 DAOQWE010000000
VERSION     DAOQWE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30811_length_3279_cov_2.561104"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PKK37_03275"
     CDS             <1..737
                     /locus_tag="PKK37_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNR81432.1"
                     /translation="GGVGKTTAAVNIAAGMSLIGRRVLLVDMDPQAHATQSLGISEEG
                     EKRKTVFEVLDETAAAADVILDRGGLAVLPSGLTLANAELYFLGTPGREMMLSNALKA
                     IRADFEYIFIDCPPSLGVLTMNALAAATGVYVPIKTEFLPMQGITQLIKTVDLIRQRI
                     NAALKITGVFGTFYDNRRTLNREVMAAVREIFGAAVFETTIRNNVALAEAPGAGKTIF
                     EHAPGSHGAEDFSALCREIISREDGQ"
     gene            737..1642
                     /locus_tag="PKK37_03280"
     CDS             737..1642
                     /locus_tag="PKK37_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNR81433.1"
                     /translation="MAKTANVANSAIAKELKAAKEAADRGELSNELTHVLAADRPAPE
                     GVKLEAVKMHAVQNLTEHPLNREYFQPLPADEYEKLKEDIQRRGVLVPVIATAAGVIL
                     SGHRRAQITRELGRTFIPVQFIAGKLTEAQEREFLIKDNLLRRQLGPEEKKALIVAVY
                     GEEIDRDRRGGDRRSAAAKSKVQSELLIHGEPSATLPERIERETGGAITAGTGRRLIA
                     EIKRDRQRAAAGEPATAPEAKPAADRLKAVRANLGKIVRLLNDADAGTVDAAIIEIVG
                     VLDQIGGIDPRYSSEVKRIIENMRA"
     gene            1645..1866
                     /locus_tag="PKK37_03285"
     CDS             1645..1866
                     /locus_tag="PKK37_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR81434.1"
                     /translation="MAIKIGPKTFYTPGEVGEILGIHAVTVRNLMRAGRLPAHKFGGR
                     WYISEDKLSAAFSGDWQPERPDEPAGEGE"
     gene            1907..2296
                     /locus_tag="PKK37_03290"
     CDS             1907..2296
                     /locus_tag="PKK37_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81435.1"
                     /translation="MIELEPETMTAAPRTRADFSENGQMSEIAKTFRRQPNPPAPVLI
                     ETPETVFSAAVRSINKRFQLGTLEYIEDHAHELAEDIRRAFDRLNQVWAGWIPGADLS
                     PFKLALRTYYNLIIRAVDIFEGATNGK"
     gene            2286..>3279
                     /locus_tag="PKK37_03295"
     CDS             2286..>3279
                     /locus_tag="PKK37_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81436.1"
                     /translation="MANKRKKVYTNGEHPNIEGVLTPDGYKSQALDVIAKRQEDGPAG
                     RIMAPPDYIFPAEDSNLQIITARIGGNNVVSQVIQIDNRVFRVLWSRYQEETAEINKE
                     QKTELQAALDWQLEKIKPQIRDDGSAGSLTRIGAFFLILGELIDGRALPARATVTDFL
                     ESAVSFLREKENIWDWLTRWQDIARGIKPKSNILDKSVLLDLTTHKNASTWIGVRGAF
                     TDVQRYRWDEDIIESEEKALGRKLTKEERINVLRKEKEYLMKSSVTPGEIFVISDRWG
                     DFSISMSVPQLFAGIKNEERRRELEGRLKNPKFFDTPEFVAGLNAELSKIILYQN"
BASE COUNT          837 a          847 c          977 g          618 t
ORIGIN      
        1 agggcggcgt tggcaaaaca accgcggccg ttaatatcgc cgccggaatg tcgctgatcg
       61 gccgccgggt tctgttggtc gatatggacc cgcaagcgca cgccacgcaa tcgctgggaa
      121 tatcggagga gggcgaaaag agaaagacgg tttttgaggt tctggatgaa accgcggccg
      181 cggcggatgt tatcctggac cgcggcgggc tggcggtcct cccgtccggg ttgacgctgg
      241 cgaacgctga actttatttc ctggggacgc cgggccgtga aatgatgtta tcgaacgcac
      301 taaaagctat ccgcgcagac tttgaatata tttttattga ttgtcctccc tccctgggcg
      361 ttctgacaat gaacgctttg gccgccgcaa ccggcgttta tgtcccaatc aaaaccgaat
      421 ttctcccaat gcagggaata acgcagctga taaaaacggt cgatctgatc cgccagcgaa
      481 taaacgcggc gctgaaaata accggcgttt tcgggacgtt ttacgacaac cgccggacgc
      541 tgaaccggga ggtaatggcg gcggtccggg agatcttcgg cgcggccgtc tttgaaacga
      601 caatccggaa taacgttgcg ctggcggagg cgccgggagc gggaaagaca atatttgaac
      661 acgcgccagg atcccacggc gcagaggact ttagcgcact ttgccgcgaa atcatttcca
      721 gggaggacgg tcaataatgg caaaaacggc gaacgttgca aactccgcta tcgctaaaga
      781 actgaaagcg gcgaaagagg cggcggaccg cggcgaactt tcaaacgaat taacccacgt
      841 tctcgcagcg gatcggccgg cgccggaggg cgtcaagctg gaggcggtca agatgcacgc
      901 ggtccagaac ttgaccgaac acccgttaaa ccgcgaatat tttcagccgt tgccggcgga
      961 cgaatacgaa aagctgaaag aggatatcca gcggcgcggc gtcctggttc cggttatcgc
     1021 aaccgcggcc ggcgtcattc tatccggtca ccggcgggcg cagataaccc gcgaacttgg
     1081 gcggacgttt atcccggtcc agtttatcgc cggcaagctg acggaggcgc aagagcgcga
     1141 atttctgata aaagataatc tgttacggcg ccagcttgga ccggaggaaa agaaagcgct
     1201 gattgtcgcc gtatacgggg aggagataga ccgggaccgg cgcggcgggg atcggaggag
     1261 cgcggccgca aaatcaaaag ttcaatctga acttttgatc cacggcgaac cctccgcaac
     1321 cctcccggaa cggatcgaac gggaaaccgg cggcgctatc acggccggga ccgggcgccg
     1381 gctgattgcg gaaatcaaga gggaccgcca gcgcgcggcg gccggcgaac ccgccacggc
     1441 gccggaggcg aaaccggctg cggaccggct gaaagcggtt cgcgcgaact tgggaaaaat
     1501 tgtccggctg ttgaatgacg cggacgccgg gaccgttgac gcggcgataa ttgagattgt
     1561 cggcgtcctg gaccagatcg gcgggatcga tccccgttat tccagcgaag taaaacgaat
     1621 cattgaaaat atgagggcgt aaaaatggct atcaagattg ggccgaaaac attttacacg
     1681 ccgggcgaag tgggcgaaat cctggggatc cacgccgtca ccgtccgaaa tcttatgagg
     1741 gccggccgcc tcccggcgca caaattcggc ggccgttggt acatttcgga ggacaagctt
     1801 tccgcggcgt tttctggcga ttggcaaccg gagagaccgg acgaacccgc cggcgagggc
     1861 gaataacttt gaatgtccgt aaatattaca ttgcagacaa tgaaaaatga ttgaacttga
     1921 acccgaaacc atgaccgcgg cgccgcgaac ccgcgcggac tttagcgaaa acggacaaat
     1981 gtccgaaatc gctaaaactt tccgccggca accgaacccg ccggcgccgg tcctgatcga
     2041 aacgccggaa acggttttct ccgcggcggt ccggtcgatc aataagcgct ttcagctggg
     2101 gacgctggaa tatatagagg accacgcgca cgaactggcg gaggatatcc ggcgggcgtt
     2161 tgaccggtta aatcaagttt gggccggctg gatccccggc gccgatctat cgccgttcaa
     2221 gctggcgttg cggacttatt acaatttgat tatccgggcg gttgatatat tcgagggcgc
     2281 gacaaatggc aaataagcgg aaaaaagttt acacgaacgg cgaacacccg aatatagagg
     2341 gcgttctcac cccggacggc tacaaatcgc aagcgctgga cgttatcgca aaacgccagg
     2401 aggacggacc ggccgggcgg attatggcgc cgccggacta tattttcccg gccgaagatt
     2461 caaacctcca gattataacc gcgcggatcg gcgggaataa tgtcgtttcg caagtaattc
     2521 agatcgataa ccgggttttc cgggttcttt ggtcacgcta tcaagaggaa accgcggaaa
     2581 tcaacaaaga acaaaaaacc gaactccagg cggcgcttga ttggcagctg gaaaagataa
     2641 aaccacaaat tcgggatgac ggcagcgccg gctctttaac gcggatcggc gcttttttcc
     2701 tgatcctggg tgaactgata gacgggcggg cgctcccggc gcgggccacg gtcaccgatt
     2761 ttctggaaag cgccgtttcg ttcctccgtg aaaaagaaaa tatctgggat tggttgaccc
     2821 gctggcaaga tatcgccaga gggataaaac cgaaaagcaa tattctggat aaatctgttt
     2881 tattggatct aacgacacat aaaaacgcaa gtacgtggat cggcgttcgc ggcgctttca
     2941 cggacgttca aagatacaga tgggacgagg atataattga aagcgaagaa aaagcgctgg
     3001 ggcggaaatt aaccaaagag gaacgaataa acgtcctccg gaaagaaaaa gaatatttaa
     3061 tgaaatcctc cgttacaccc ggcgaaatct ttgttataag tgaccgttgg ggagattttt
     3121 ctatttcaat gtccgttccg caattattcg ccgggatcaa aaacgaagag cggcgccggg
     3181 agctggaggg gcggctcaaa aatccgaaat ttttcgacac gccggaattt gtcgccgggc
     3241 tgaacgccga actttcaaag attattcttt atcaaaacg
//
LOCUS       DAOQWE010000088         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_31017_length_3263_cov_7.797070, whole genome shotgun sequence.
ACCESSION   DAOQWE010000088 DAOQWE010000000
VERSION     DAOQWE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31017_length_3263_cov_7.797070"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKK37_03300"
     CDS             complement(<1..751)
                     /locus_tag="PKK37_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81437.1"
                     /translation="MREICKMPDNDTESIREIPGFAVLKGPLLDNDIELLEYEGSKMA
                     NFDLNLDIPGWPPRNKEDERQLAGFLGLNGAQDIIIPPAWNRGGIIQTDKGDIVIKGF
                     CISPDFKRKQLKGKYDEFIEENFKRYDEQHFGKLKMASALTEVRRALNAIKLGYKQPE
                     PISIFKVIDDRSNAENPNVVYARLMQNGIRVADLMMLKEIGGSEATEKLLDFSIKKLP
                     QEFKITNKDEYINHIIDELTGELTKRNENKFI"
     gene            complement(1243..1884)
                     /locus_tag="PKK37_03305"
     CDS             complement(1243..1884)
                     /locus_tag="PKK37_03305"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNR81438.1"
                     /translation="MASKNEKYSKMEANDAIIAAGIGGGVAVYFYYILGQSSGGLIPE
                     SFVRWRWLLLIVFPLLAALGLQIAFLIGKKFLAIYQLAKFLLVGAMAAIVDLTLLNVF
                     MILSGAASGAGFNVAKGISFAAATSLKYFPDKLWAFKNKNRENLKREFGKFFAITLAG
                     LALNIFIADIIVNQIGPQWGIPAELWANLGGIGAIMAVFIFNFFGYKILVFKK"
     gene            2179..3114
                     /locus_tag="PKK37_03310"
     CDS             2179..3114
                     /locus_tag="PKK37_03310"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNR81439.1"
                     /translation="MKILETIGNTPLVEIEKLNPNPKVKIYAKLEGANPTGSIKDRIA
                     LAMIEHAEKTGALKKGKTIIEATSGNTGIGIAMVAAIKGYKTMIVLPESMSVERQKMI
                     LAFGAELILVKPEMWRDGAVAMIKEMAAKDKKLVLLNQFDNEQNCLAHYRTTGKEIVR
                     QVPGKIDYLIAGLGTGGTITGIARRLREKHPAMRVIGIQPYLGEKIEGLRSVKSGAIP
                     KILEPCLPCGDHRKSLIDIVIEVHEKDAVNTVRGLAAKEGIFVGQSSGAAMFAAAKYA
                     KKAGKGTIVTVFPDRGEKYLSTGVFSSSKNNRPTA"
BASE COUNT          904 a          700 c          743 g          916 t
ORIGIN      
        1 atatgaattt attttcattt cttttggtaa gctcgccggt caactcatca attatatggt
       61 taatgtattc atctttattg gtaattttga attcttgcgg cagttttttg attgaaaaat
      121 ccaataattt ctcggtagct tcgcttcctc ctatttcttt taacatcatg agatcggcga
      181 ctctgattcc gttttgcatc aatcttgcgt agactacatt tggattttcc gcgtttgacc
      241 ggtcatcaat aactttaaaa atagatatgg gttccggttg cttgtagcca agtttgattg
      301 cattgagcgc ccgccggacc tccgtgagcg cgctggccat ttttaatttt ccaaaatgct
      361 gctcatcgta ccttttgaaa ttttcttcta tgaattcatc gtatttacct ttaagttgtt
      421 tccttttaaa atcgggactt atgcaaaacc ctttaataac tatatctcct ttatccgttt
      481 gtattattcc gccccggttc catgcgggcg gaataataat gtcttgggca ccattcaatc
      541 ccaaaaaacc ggccaattgt ctttcatctt ctttattcct tggcggccac cccgggatat
      601 ctaagtttag atcaaaattg gccatttttg acccttcata ttccaataat tctatatcat
      661 tatccaaaag cggtcctttt aaaactgcga atccggggat ttccctgatg gattccgtat
      721 cattatcagg cattttgcat atttcgcgca ttgcattttt ataattagtt tacacaaggg
      781 ttaaaatctg acctgatttc ccgctgaaaa acttgacaaa attaaatata tatatattat
      841 ataattatca aggcgattga attgcgaaaa aagttgatgt ggaggcgggg aagcttatcg
      901 gtggttttgg gtattacctt ggtggttttg tgtattacct tggtagtttg gagtgttgca
      961 atcttggtgg gctttcctga tgttactttc ggcgagcgat ttatgggggg ttgtgctttt
     1021 attgtgggct gtttcttcat gtatgagata ttattcagtg attagttaca tgctaaaagc
     1081 cttccgtgaa agatttcata cccccctttt ttttatatat tcgctattaa attagttggg
     1141 attattttaa tttattgacg tctaacgatg tcgccgaagg tttgcggccg gtatttgcga
     1201 tataaaacta aattttccat aaaatatgtt aaagctatat tattattttt tgaagacgag
     1261 gattttgtag ccgaaaaagt tgaagatgaa aaccgccatt attgcgccga taccgcccag
     1321 gtttgcccaa agttcggcgg gaattcccca ttgcggcccg atttgattga cgatgatgtc
     1381 ggcgataaaa atgttgagcg ccaacccggc cagagtgatg gcaaaaaatt tgccgaattc
     1441 gcgttttaga ttttcccggt ttttgttttt aaacgcccac agtttgtcgg gaaaatattt
     1501 cagcgatgtg gcggcggcaa aagatattcc cttggccaca ttgaagccgg cgccggacgc
     1561 cgcgccgctc aagatcataa agacgttgag cagagtcaga tcaacgatcg cggccattgc
     1621 tcccaccagc aaaaatttgg ccaactggta aattgccaga aactttttgc cgatcagaaa
     1681 cgcaatttgc aggccgagag ccgcaagcaa aggaaaaacg atcagcaaaa gccatcgcca
     1741 acggacgaag ctttccggta tcaaaccgcc cgaagattgt cccaaaatat aataaaaata
     1801 aaccgccact ccgccgccga tgcccgcggc aatgattgcg tcatttgcct ccatttttga
     1861 atatttttcg tttttggatg ccattgccga ttttaccaca gattggcttt cgcggacata
     1921 ttgacaagga tgggttggtc ttgttatgct ggaagagcaa gattatgaac aagaaacttc
     1981 gttcaaagaa atcggtccgc ttgtgtatga tcctctcgta aagagcggcc ggttttggtg
     2041 aatttgtttg cgacaatgta tgctaccgcg cctctcttta cgggaggcgc gtttcaattt
     2101 gaaaattaaa aaagatacgg gaagtgctac gggcaatcat aaaggttgcc cctacaaaaa
     2161 aaatacaaaa ttaaaattat gaaaatattg gaaacaatcg gcaatactcc gttggtggaa
     2221 attgaaaaac tcaatcccaa tccgaaagtg aaaatctatg ccaaattgga aggcgccaat
     2281 cccaccgggt caatcaaaga ccgcatcgcc ttggcgatga ttgaacatgc cgaaaagacc
     2341 ggcgcactaa aaaagggaaa aacaatcatt gaggcgacca gcggcaacac cggcatcggg
     2401 atcgccatgg tggcggccat caaaggctac aagacgatga tcgttctgcc cgaatcaatg
     2461 agcgtggagc ggcaaaaaat gatactggct ttcggcgccg aactgatatt ggtcaagccg
     2521 gaaatgtggc gcgacggcgc ggtggcaatg atcaaagaaa tggcggccaa agacaaaaaa
     2581 ctggttctgc tcaaccagtt tgacaacgag caaaattgtt tggcgcatta tcgcaccacc
     2641 ggcaaggaaa ttgtccggca agttccggga aagattgact atttgatcgc cggactgggc
     2701 accggcggca cgatcaccgg catcgcgcgg cggctgaggg aaaaacatcc cgcaatgcgc
     2761 gtcatcggca tccagcccta tctgggcgaa aaaatagaag gattgcgctc ggtcaaatcc
     2821 ggcgcgattc ccaaaattct ggagccttgc ctgccctgcg gcgaccatcg caaaagcctg
     2881 attgatatcg tgattgaagt tcatgaaaag gacgccgtta acaccgtccg cggtctggcg
     2941 gcaaaagaag gaatttttgt cgggcaaagc tccggcgcgg cgatgtttgc ggccgccaag
     3001 tatgctaaaa aagcgggaaa gggaacaatc gtcaccgtct tccccgaccg cggcgaaaaa
     3061 tatttgagca ccggagtttt ttcttcatca aaaaacaata gacctactgc gtaaatattt
     3121 acgcaacgaa attttcagat ttcgcgcgag ccgaatgagg aatgaggagg catattggct
     3181 taaatatgcc gacgattgac gaattcggcg tagccgaaat atggaaattg cagtaggaaa
     3241 tatttacgca gtaggtctaa taa
//
LOCUS       DAOQWE010000089         3206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_31811_length_3206_cov_9.308474, whole genome shotgun sequence.
ACCESSION   DAOQWE010000089 DAOQWE010000000
VERSION     DAOQWE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31811_length_3206_cov_9.308474"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PKK37_03315"
     CDS             complement(<1..1332)
                     /locus_tag="PKK37_03315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNR81440.1"
                     /translation="MDYLQNIISLAKRRGFVFPSSEIYGGFAAVYDYGHYGTLLKNNI
                     RDAWWRHMVQLRNDIVGLDSAIFMHPVTWKASGHVDSFDDPQVDCKKCKSRWRADHIL
                     EQFGVSADKQPLEFINAELDKLRESKKLKCANCGSADLTPAKRFSLMVKSNLGSPTDA
                     LSEENVVYLRPETCGGIYLEYKNTLDTARVKLPFGIAQVGKAFRNEIVARQFIFRTRE
                     FEQMEMQYFLHPEQMKEKYEEWKKVRWEWYLEYGMGESDIRWYQHEKLAHYASEAYDI
                     EYNFKALGGYKEVEGIHARGDWDLSQHSKFSGQDLTYADPQTGEKFIPHIMETSVGLN
                     RLFFMFMDKAYAEETVNGETRTVLKFDKRLSPIKAAIFPLLKNKPELVAKAREIYDAL
                     KPEFMCEFDDSGNIGKRYRRQDEIGTPYCITVDFDSLENGDVTVRDRDTMAQ"
     gene            complement(1505..3184)
                     /locus_tag="PKK37_03320"
     CDS             complement(1505..3184)
                     /locus_tag="PKK37_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81441.1"
                     /translation="MSKFFKVLIVFALVLAAVGYGFWRKNIYSKEELKLEIFGPREAA
                     LGQEVEYVVKYKNNGDFRLENPSLVFTAPDFSIKDDKIFAKEVLDAEKLGGAIYPGEE
                     KSYTFKFRVAGKAGEAKIAKASVSYQPKNLSARYESSTTFTTIMKEAPLDLEMDLSST
                     VEADKSFHLKINYFSNVDWLLTDLRVSVDYPAGYSFSQSSPKSFDKNEWVIPVLNKNQ
                     SGTIDIVGQLSGSLDEGKVFRARIGMWKDGQYMQLREIEKGARIVKPTVALRQTINGD
                     ANYTAKPGEWLHYEVYYKNVSDKELYNLVLVDSLEGDLFDLGTIKSETGSFRPGDNSL
                     VFDWKQNSQLAYLPMANEGKVEFWIKLKDDLSRLNRPELANKVMVGPAREDFVTKISS
                     KLELAQKAFYSDEIFGNTGPLPPQAGTATTYTVTWQAKNYYSNVKEVAVRATLPPEAQ
                     FVPGKIFPEIQTAGLTYNEATREIVWNIGDMAAGQGLVSNPLNVSFQIAVSPMSSQIG
                     GTAGIIGQAVIKGGDEWTEASLESVSPALDTASLSDPILIENSGIVQPKPN"
BASE COUNT          751 a          846 c          753 g          856 t
ORIGIN      
        1 ttgcgccatg gtgtcgcggt cgcgcacggt gacatcgccg ttttccagcg aatcaaaatc
       61 cacggtgatg caatagggcg tgccgatctc gtcctggcgg cgatagcgct tgccgatgtt
      121 gcccgaatcg tcaaattcgc acataaattc gggttttaac gcatcataaa tctcgcgcgc
      181 cttggcgaca agttcaggct tgtttttcaa caaggggaag atcgcggctt taatcgggct
      241 cagccgctta tcaaatttaa gcaccgtgcg cgtttcgccg ttgacggttt cttcggcata
      301 ggccttgtcc ataaacataa aaaacaatcg attcaagccc acggaggttt ccataatatg
      361 gggaataaat ttctcgccgg tttgcggatc ggcatacgtt aaatcctggc cggaaaattt
      421 ggagtgctgg ctcaaatccc aatcgccgcg cgcgtggata ccctccactt ctttgtagcc
      481 gcccagcgcc ttgaaattat attcaatatc ataggcctcg gaagcgtagt gcgccaattt
      541 ttcgtgttgg taccagcgga tgtcgctttc acccatacca tactccaaat accattccca
      601 gcggactttt ttccattctt catatttttc tttcatctgc tctgggtgca ggaaatattg
      661 catttccatt tgttcaaatt cgcgcgtgcg gaaaatgaat tgccgcgcga cgatctcgtt
      721 gcgaaacgcc ttgcccactt gcgcgatgcc gaacggcaat ttcacccgcg cggtatccag
      781 cgtgttttta tattccaagt aaataccgcc gcaagtttcg gggcgcagat agaccacgtt
      841 ctcttcgctt aacgcgtcgg tgggcgaacc cagatttgat ttcaccatca gggaaaatct
      901 tttggccggc gtcagatccg ccgacccgca attggcgcac tttaattttt tggattctct
      961 caatttatca agttcggcat tgataaattc cagcggctgt ttgtccgccg acacgccgaa
     1021 ttgttccaga atatgatccg cgcgccagcg ggatttgcat tttttgcagt ccacctgcgg
     1081 atcgtcaaag ctgtccacat ggccggacgc tttccaagtt acggggtgca taaaaatcgc
     1141 gctgtccaga ccgacaatgt cgttgcgcaa ctgcaccata tgccgccacc acgcgtcacg
     1201 gatgttgttt ttcagcagtg ttccgtaatg gccgtaatcg tagaccgcgg caaaaccgcc
     1261 gtaaatctcc gatgacggaa aaacaaaccc ccgcctcttg gccaacgaaa tgatattttg
     1321 caagtaatcc acgtttcaaa tgaaaattta aaatatcaaa atgaaaaatt aaggtaattt
     1381 taaaaataga cttgcacgcg cgattacacg agggatgaac ccttgccttg cgcctatttt
     1441 ggattaagcg tgtgagccaa ttttaaattt taaattgtca ttttgatttt tgatttttca
     1501 atttttaatt tggtttgggt tgcacgatgc cgctgttttc aatcaatatc gggtcggaaa
     1561 gcgacgcggt gtccaacgcc ggcgacaccg attccagcga ggcctccgtc cattcatcgc
     1621 cgcccttgat caccgcctgg ccaatgattc cggccgtccc gccgatttgc gaactcatcg
     1681 gcgacaccgc gatttggaaa gaaacgttca gcggattgga aaccagcccc tgcccggcgg
     1741 ccatatcgcc gatattccaa acaatttcac gggtggcttc gttataagtc aagccggcgg
     1801 tttgtatctc ggggaaaatc ttgccgggaa caaattgcgc ctccggcggc aaagtcgcgc
     1861 ggacggccac ctctttaacg tttgaataat aatttttggc ctgccaggtg acggtgtagg
     1921 tggtcgccgt tcccgcttgc ggcggcaaag gcccggtatt gccgaaaatt tcatcgctgt
     1981 aaaaagcttt ctgcgccagc tccaattttg aactgatctt ggtgacaaaa tcttcccgcg
     2041 ccggcccaac catcactttg ttggccagct ccggacgatt caagcggctc aagtcgtctt
     2101 tcagcttgat ccagaattca accttgcctt cgttggccat cggcaaatag gccagctggg
     2161 aattctgttt ccagtcaaac accagcgaat tgtctcccgg acggaaactg ccggtttcgg
     2221 acttgatggt gcccaggtca aacaagtcgc cttcaaggct gtccaccaaa accagattgt
     2281 agagctcttt gtcgctgacg tttttgtaat aaacttcata gtgcaaccat tcgccgggtt
     2341 tggcggtgta attggcatcg ccgttgatcg tttggcgcag ggccaccgtg ggtttgacga
     2401 tccgggctcc tttttcaatt tcccgcaact gcatatactg gccatctttc cacattccga
     2461 tgcgggcgcg gaaaaccttg ccttcgtcca gcgacccgga aagctgtccg acaatgtcaa
     2521 tcgtgccgga ctggtttttg ttcaaaaccg gaatcaccca ttcgttcttg tcaaaagact
     2581 tcggggaaga ctgcgaaaag gaatagccgg cgggataatc cacgctcacg cgcaaatcgg
     2641 tgagcaacca gtcaacgttg gaaaaataat ttatctttaa atgaaaactc ttgtccgctt
     2701 ccacggtgga ggaaagatcc atttccagat ccagcggcgc ttctttcata atcgtggtaa
     2761 aagtggtgga cgattcatag cgcgcgctca aatttttggg ctgataggaa accgacgcct
     2821 tggcgatttt ggcttcgccg gcttttcccg ccacgcggaa tttgaaggta tagctttttt
     2881 cttccccggg atagatcgcc ccgcccaatt tttcggcgtc aagcacttct ttggcgaaaa
     2941 ttttgtcgtc tttgatggaa aaatccggcg ccgtgaacac caaactcggg ttttccaaac
     3001 ggaaatcgcc gttgtttttg tatttgacca catattccac ttcctgcccc aaggccgctt
     3061 cgcgcggtcc gaaaatttcc aatttcaatt cttccttgga gtagatgttt ttgcgccaaa
     3121 agccgtaacc cacggccgcc agcaccaacg cgaaaacgat taaaacttta aaaaatttgc
     3181 tcattggaaa atgataaaat tatttt
//
LOCUS       DAOQWE010000090         3202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_31882_length_3202_cov_6.992374, whole genome shotgun sequence.
ACCESSION   DAOQWE010000090 DAOQWE010000000
VERSION     DAOQWE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31882_length_3202_cov_6.992374"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1333
                     /locus_tag="PKK37_03325"
     CDS             227..1333
                     /locus_tag="PKK37_03325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNR81442.1"
                     /translation="MKIQNLKIMIVAIICLALAAVLGLAARDGNIKNTGVPTMETAPD
                     GASAVAGGALAAENQPAGAGKTAEAPMEILAFGDLLLDRYIKLYIDRHGADYPFENIK
                     EIFAGNDIVMANLEGSFTDFAPRPLDPDNTSFTFNPELAAMLKRHRFNVVNLANNHVQ
                     DFGKAGFSQSGNYLTQNGIDYFGDYYNESPALIKEINGQKIAFVGYHSLADPATEGVV
                     EKIKQAHEIADYIIVYTHWGAEYQNDFSKSQQAAGREFIDAGADAVLGSHPHVIQPIE
                     VYNNKPIFYSLGNFLFDQIFSFDVRHGLGVKIIFASNEIEYELIPTQMKNFQVVPPDE
                     AAKTLILQNLSAKSVASDDIKAQISSGKFTLSDN"
     gene            complement(1414..>1689)
                     /locus_tag="PKK37_03330"
     CDS             complement(1414..>1689)
                     /locus_tag="PKK37_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HNR81443.1"
                     /translation="QIEVVDLSEARARQNEIKSEMECIRAKEGLEFIALMATDIINCG
                     SEIFVAGNPDYIQKAFGKIPANGNLYIKGMMSRKKDLLPPLLKTLEK"
     assembly_gap    1690..1789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1790..2377)
                     /locus_tag="PKK37_03335"
     CDS             complement(<1790..2377)
                     /locus_tag="PKK37_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNR81444.1"
                     /translation="MAKDTVYVIGHKSPDTDTVCSAIAYAEYLKKKQKIGTVAAVCGD
                     LNPETKFVLDYFKIKKPAKLNSAAGKTLVLVDHNERSQMPDGADSAKIIEVVDHHKIC
                     FEYPEPIIFRAEPLGSTATLTAKIMIAGKFKIAKPMAGILLAAILSDTVVFRSPTCTA
                     EDVKIAKLLAKTAGVKDIKKFGVEIKKQKASLKKMT"
     gene            2455..>3202
                     /locus_tag="PKK37_03340"
     CDS             2455..>3202
                     /locus_tag="PKK37_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNR81445.1"
                     /translation="MDTVNYLILGLLQGVFEWIPISSEGVVALAARYLGAEQNPVDLA
                     LFLHAGTLLAALIYYRRDWMRILTLKDWPLIRFLAIATAVSLPLGFAVSRIAAQAVMG
                     AGLLALTGVGLLFTAYFQAKKINLKLGKNKLAGIAGVLQGLAALPGFSRSGSTIFGLS
                     LGNLPPAQVLRLSYLMSVPAVAASTAYLALFEAEQFTLTVWPALAASFAVGILTLDLL
                     TKFAEKINFAKFALAFALLCFAGAALELAMR"
BASE COUNT          711 a          655 c          866 g          870 t
ORIGIN      
        1 cggagctttt gagtttgcgt gcccgcgggc gctgggtatt gcattttgca tctattcctc
       61 ctttggaaaa ggaggtgccc cgccgcggcg cggcggagga tttttaaaat ccagaagtta
      121 tcaggggtcc aacccctgta aatttaacag gggttgaacc cctggaggct ggggttgcaa
      181 aacgggggag gcagagtagg ataaaattaa aaatgaaaaa ttaaaaatga agattcaaaa
      241 tttaaaaata atgattgttg cgataatttg tttggcgctg gcggcggttt tggggttggc
      301 ggccagagac ggaaatatta aaaataccgg cgtgccaacg atggaaacgg cgccggacgg
      361 ggcatcggcg gttgcggggg gagcgctggc ggcggaaaat caacccgcgg gggcgggaaa
      421 aacggcggaa gcgccaatgg aaattttggc gttcggcgat ttgctgttgg atcgctatat
      481 caagctttat attgaccgcc atggtgcgga ttatcccttt gaaaacatca aagaaatttt
      541 tgcgggcaac gatattgtga tggcgaattt ggaaggatcg ttcacggatt ttgcgccgcg
      601 gccgcttgat cccgacaaca cgagttttac ttttaatccc gaattggcgg cgatgctcaa
      661 acggcaccgc ttcaatgttg taaatctggc caataaccac gtgcaagatt ttggcaaggc
      721 gggtttctcg caatcaggaa attatttgac tcaaaacggt attgattatt ttggcgatta
      781 ttacaatgaa agtccggcgc tgataaaaga aattaacggc caaaaaatcg cgtttgtcgg
      841 ttatcattcg ctggccgatc cggcaacgga gggcgtggtt gagaaaataa aacaggcgca
      901 cgagatcgcc gattatatca ttgtctacac tcactggggc gcggaatatc aaaacgattt
      961 ttcaaaaagc cagcaggcgg ccggccgcga attcattgac gcgggcgcgg atgcggtttt
     1021 gggaagccat ccgcatgtga tccaaccgat tgaggtctat aacaacaagc cgatattcta
     1081 ctcgctgggc aattttttgt ttgaccagat tttttcgttt gacgtgcgcc acggtttggg
     1141 cgtgaaaatc atttttgcgt ccaatgagat tgaatatgag ctgattccca ctcaaatgaa
     1201 aaattttcag gttgtcccac ccgacgaagc ggcaaaaacc ttgattttgc aaaatctgtc
     1261 ggcaaaatcc gtcgcttccg acgacatcaa agcgcaaatc tcctccggca aattcacgtt
     1321 gtctgataat taaactttgt gcaaccccgg ttaacaaaca ttccaacatt cttgagaatg
     1381 ttggaatgtt tgttaaccgg tcttattcgg cgtttatttt tccaaagttt tcagcagggg
     1441 cgggaggagg tcttttttgc gcgacatcat tcctttgata tagagattgc cgttagcggg
     1501 aattttgccg aaagcttttt gaatatagtc ggggttgcca gcgacaaaaa tttccgaacc
     1561 gcagttgatg atgtcggtgg ccatcagggc gatgaattcc agtccttcct tggcgcggat
     1621 gcattccatt tcgcttttta tttcattttg gcgggcgcgg gcttctgaca gatccacaac
     1681 ttcaatttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtcatttttt
     1801 tcagcgacgc tttttgcttt ttgatctcaa cgccgaattt tttaatgtct ttgacgccgg
     1861 cggtttttgc cagcagtttg gcgatcttga cgtcttcggc ggtgcaggtg ggggaacgaa
     1921 acacgaccgt atcggacaat attgccgcca gcaaaatgcc ggccatgggt ttggcgattt
     1981 tgaatttgcc ggcgatcatt attttggcgg tcagcgttgc cgtggacccc agcggttcgg
     2041 cgcggaaaat tatgggttcg ggatattcaa aacaaatttt gtgatggtca acgacttcaa
     2101 tgattttggc ggagtcggcg ccatcgggca tttgcgatct ttcgttgtga tccactaaaa
     2161 cgagcgtttt gcctgccgcg gaattgagtt ttgccggttt tttaatttta aaataatcaa
     2221 gaacaaattt ggtttcggga ttaagatcgc cgcaaaccgc cgcgacggtg ccgatctttt
     2281 gttttttctt caaatattcg gcataggcga tggccgagca aacggtgtcg gtgtcgggcg
     2341 acttgtggcc aatcacataa acggtatctt ttgccatagg gaggttttaa ttaggatggt
     2401 tatatattag aataaacaag tgcggttgta aaattttcaa aatttgaatt tatcatggat
     2461 actgttaatt atcttattct gggtctactg cagggggtgt ttgaatggat tcccatttct
     2521 tcggaggggg tggtggcgct ggcggcgcgc tatttgggcg ccgaacaaaa tccggtggat
     2581 ctggcgttgt ttttgcacgc gggaacattg ctggcggcgc tgatctatta tcgtcgcgat
     2641 tggatgcgga ttttaacgtt gaaagattgg ccgttaattc gatttttggc catcgcgacc
     2701 gcggtttcat tgccgctggg atttgcggta agccgcatag cggcgcaggc ggtgatgggc
     2761 gcgggattgt tggcgctcac cggtgtcgga ttgcttttta ccgcgtattt tcaagccaaa
     2821 aagatcaatt tgaaattggg caagaacaaa ttggccggca ttgccggagt tttgcaggga
     2881 ttggcggcat tgccgggatt ttcccgttcg gggtccacga tattcggctt gtcgctggga
     2941 aatctgccgc cggcgcaagt gttgcgctta tcctatctga tgtcggtgcc ggcggtggcg
     3001 gcctccaccg cgtatctggc gctgtttgaa gcggagcaat tcactctgac agtctggccg
     3061 gcgctggcgg ccagttttgc cgtcggcatt ttgacgcttg atttgctgac aaaatttgcc
     3121 gagaaaatca atttcgccaa attcgcactg gcgtttgcgc tgttgtgttt tgccggcgcg
     3181 gcgctggaat tggcgatgcg at
//
LOCUS       DAOQWE010000091         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_32200_length_3179_cov_6.772087, whole genome shotgun sequence.
ACCESSION   DAOQWE010000091 DAOQWE010000000
VERSION     DAOQWE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32200_length_3179_cov_6.772087"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PKK37_03345"
     CDS             complement(<1..798)
                     /locus_tag="PKK37_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNR81446.1"
                     /translation="MKIAINGLGRIGRHVFKIIIDKHPELEIAAINDLTDAQTIAHLL
                     RFDSVYGRWEKEIETAKKELIVKTKKSVRRIAIFSETDPANLPWKKLGVEVILECTGR
                     FVKYDDAAKHLIAGAKKVIISAPSKDADKVPSYVLGVNADQYNPAKFDVMDMGSCTTN
                     CLAPVAKTLLDNFGIVKGFMTTVHAYTNDQKILDVAHKDLRRARAAGLNIIPTSTGAA
                     KAIGKVIPELKGKLDGVALRVPVATGSMVDLVCELERETTAEEINSIL"
     gene            complement(853..1083)
                     /locus_tag="PKK37_03350"
     CDS             complement(853..1083)
                     /locus_tag="PKK37_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNR81447.1"
                     /translation="MIKVYSTPFCPYCVTLKDYLKEKKVAFSEVDVSENEKERDEMVE
                     LSGQIGVPVINIDGKIIVGFDKTEIDATLGLK"
     gene            complement(1096..2130)
                     /locus_tag="PKK37_03355"
     CDS             complement(1096..2130)
                     /locus_tag="PKK37_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81448.1"
                     /translation="MAIKISKSVLYPVLSLCGFAVISLLAYFQPWKDMGMIGKTIKPE
                     AAAQRAIDYINENMLSPEIRAEYADVKEENGMYSFKLVIQGQNYASYVTKDGALLFAE
                     GVPQAIILDKKLEEEKAAAEIDKSDRPEVKLFVMSYCPYGLQAQKMYLPVYELLKDKA
                     DMGIYFVNYAMHGKAEIDENLRQYCIQKEQPEKVAAYLKCFAADTKYAECSTEDCTAD
                     YGKCLAEAGIDQNQLSSCMAATDKEFNITADYNDKSTWVSGVYPKFAVNDDLNTQYEV
                     QGSPTIIINGKDASGAISSRSPEKFKQAVCAAFNEQPEECNTVLSEEQPSSGFGAAVS
                     ASDSAAGSCN"
     gene            complement(2229..2450)
                     /locus_tag="PKK37_03360"
     CDS             complement(2229..2450)
                     /locus_tag="PKK37_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81449.1"
                     /translation="MMDFNEDTTLKEVLEFSKGEDVLLKYNVPCLGCPMAQMEMADLT
                     LKDICSAYRIDLRNLLIDLNLATQNAIEK"
     gene            complement(2512..3006)
                     /locus_tag="PKK37_03365"
     CDS             complement(2512..3006)
                     /locus_tag="PKK37_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81450.1"
                     /translation="MSKLSIIAIRKDLVITAALTGAAAMAPLFGQQLVTGTIVNAALF
                     LAVLLSGFRAAAAVAVVPSLIAVVAGTLPAAMAVMVPHIMASNIALAGIFTLGRRFGY
                     WLAAPIAVLAKFAILTASATVVLSAITHGNIGVALASMMGWPQLITGLLGAGLVYALF
                     KREK"
BASE COUNT          798 a          837 c          642 g          902 t
ORIGIN      
        1 caaaattgaa ttgatttctt cagcggtggt ttcgcgctcc aactcgcata ccaaatccac
       61 cattgatccc gtggccaccg gcacgcgcaa ggcgacgccg tccaatttac ctttcaactc
      121 gggaatcacc ttgccgatag ctttggccgc gccggtgcta gtaggaatga tgttcagtcc
      181 cgcggcacgc gcccgtctca aatccttgtg tgccacgtcc aaaattttct gatcgttggt
      241 ataggcatgg accgtggtca taaaaccttt gacgatgcca aaattgtcca gcaaggtttt
      301 tgccaccggc gccaggcaat tggtggtgca tgagcccata tccatcacat caaatttcgc
      361 cggattgtat tgatcggcgt taacgcccaa aacatacgac ggcactttgt cggcgtcttt
      421 tgagggcgcc gaaatgatca ccttttttgc gccggcgatc aggtgcttgg ccgcgtcatc
      481 gtatttgaca aaccggccgg tgcactccaa aatcacctcc acgcccagtt ttttccacgg
      541 caaattcgcc ggatcggttt cggaaaaaat cgcgatgcgc cggacgcttt tttttgtttt
      601 gacgatcaat tcttttttcg cggtttcaat ttctttttcc caacggccgt agacggaatc
      661 aaaccgcaaa agatgggcga ttgtttgtgc gtcagtcaaa tcattgatcg ccgcaatttc
      721 caattccgga tgcttatcaa tgataatttt aaaaacatgg cgaccaatcc ttccaagccc
      781 attaatggcg attttcataa attttaaatt aataaacaag ccgacacgca ttcgcgcatc
      841 ggcttgtttt ggttatttca atccgagagt ggcatcaatc tccgtcttat caaaaccgac
      901 gattattttg ccgtcaatgt taatcaccgg cacgccgatc tggccggata gttcaaccat
      961 ttcgtcgcgc tctttttcat tttccgaaac gtcaacttcc gaaaacgcga cttttttctc
     1021 tttcaaataa tctttcaggg tgacgcaata cgggcaaaac ggagtggaat agaccttgat
     1081 cattttgatt ttttgttaat tgcaactgcc ggcggcgctg tcggaggcgg aaaccgccgc
     1141 gccgaatccg gaagacggct gttcttcgga caaaacggtg ttgcactctt ccggctgttc
     1201 attgaaagcc gcacaaaccg cttgcttgaa tttttccggc gaccgcgaag aaatcgcgcc
     1261 ggacgcgtcc tttccgttaa taattatcgt cggcgatccc tgaacctcgt attgagtgtt
     1321 taaatcgtcg ttgacggcaa acttcggata aacgccgctt acccacgttg atttgtcgtt
     1381 gtaatcggca gtgatgttga attccttgtc ggtcgccgcc atgcacgagc tcaattgatt
     1441 ttggtcaatc cccgcttcag ccagacattt gccgtaatca gccgtgcagt cttcggtgga
     1501 acattcggca tatttggtat ccgcggcaaa acatttcaag taggccgcca ccttttccgg
     1561 ttgttctttt tgaatgcagt attggcgcag attttcgtca atttccgcct tgccatgcat
     1621 cgcatagttc acaaaataga ttcccatatc cgccttatct ttgagaagct cataaaccgg
     1681 cagatacatt ttctgcgcct gcaacccgta agggcaatag ctcatcacga acaatttcac
     1741 ctccggccgg tcgcttttgt caatttcggc cgcggctttt tcttcttcca gcttcttgtc
     1801 taaaatgatc gcctgcggca cgccctcggc aaacaacaac gccccgtcct tggtaacata
     1861 ggaagcgtaa ttctggccct gaatcaccag cttgaacgaa tacattccgt tttcttcctt
     1921 cacatcggca tattcggccc gaatctccgg cgacagcata ttttcattaa tgtaatcaat
     1981 cgctctttgc gccgccgcct ccggcttgat agttttgccg atcataccca tatctttcca
     2041 tggctggaaa taagccagca aggaaatcac cgcaaacccg cacagggaca gcaccggata
     2101 cagcacgctt tttgaaattt taattgccat attttttatt tatatttaat tattaacgtt
     2161 atcaggttac attattcctg attattttgc aacacctttc ctgaattcaa tcttgaaacg
     2221 attgtttgct atttttcaat ggcattttgc gtggccaaat tcagatcaat cagcaaattt
     2281 ctcagatcaa tccggtaggc ggaacaaata tctttaaggg taagatctgc catctccatt
     2341 tgcgccatcg ggcatcccag gcacggaaca ttgtatttca acaacacgtc ttcccctttg
     2401 gaaaactcca aaacttcttt taatgttgta tcttcgttga aatccatcat aaaaatgaaa
     2461 attaaaaaat gtgttttaaa tttttagttt ttagttttcg tttttacttt ttcatttttc
     2521 ccttttgaat agagcataaa ccaatccggc acccaacaaa cccgtaataa gttgcggcca
     2581 gcccatcatt gacgccagcg ccacgccgat attgccatgg gttatcgccg acaagacaac
     2641 ggttgcgctc gccgtcaaaa tcgcaaactt ggccaaaacg gcaatcggcg cggccagcca
     2701 ataaccgaac cgccgcccca aggtgaaaat ccccgccagc gcgatgtttg acgccataat
     2761 atggggcacc atcaccgcca ttgccgcagg caaagtccct gccaccaccg cgatcaagct
     2821 cggcaccacc gcaaccgccg cggcggcacg aaatcccgaa agcaaaaccg ccaaaaacag
     2881 cgcggcgtta acgatcgtcc cggtcaccaa ttgctggccg aacagcggcg ccatcgccgc
     2941 cgccccggtt aacgccgcag ttatcaccaa atcttttcta atcgcaatga ttgataattt
     3001 agacataata atcaaattaa aaacagaaat ttaaaaatta aaaactaaaa ctaaaaatta
     3061 aaaaccgtga acgcgcgctt taattaattt ttaattttaa attgttgttt ttaatttttc
     3121 atttttattt tttaattatt tttgatttcg cttttgcctt tttgcgcgta atgcgtgtg
//
LOCUS       DAOQWE010000092         3165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_32426_length_3165_cov_6.736656, whole genome shotgun sequence.
ACCESSION   DAOQWE010000092 DAOQWE010000000
VERSION     DAOQWE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32426_length_3165_cov_6.736656"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKK37_03370"
     CDS             <1..383
                     /locus_tag="PKK37_03370"
                     /inference="COORDINATES: protein motif:HMM:NF039287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR81451.1"
                     /translation="GIGNLAARVTTVAKKIGAAANKEYANADLKKEAGSARAARDESL
                     AQYKFNEAIAAVWKLVNSGDRYIEQTQPWKTKDAAAAGDLLLLLADFADLIEPFVPQT
                     SAKIKSLLAGENRGILFPRLDGEQ"
     gene            638..1069
                     /locus_tag="PKK37_03375"
     CDS             638..1069
                     /locus_tag="PKK37_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81452.1"
                     /translation="MVKAGAGLIEPSWKRMDVDTIFIRGIHNPGGIITLEWDRDIQGA
                     VSQLGLNANSARFQVEYLDSPMPQRDGYYRLVAIKIMVGGSGSSVMDFLEQFAMNEWR
                     RLGINFENINVIERPNSDSRVGFIICRKNDLLLVASTWVPA"
     gene            1790..3073
                     /gene="nadE"
                     /locus_tag="PKK37_03380"
     CDS             1790..3073
                     /gene="nadE"
                     /locus_tag="PKK37_03380"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HNR81453.1"
                     /translation="METTDYKIPKGKEVVFVGRFQPLHRGHLEAIKWILVQSKRLKIV
                     IGSMQEFGQADNPLEFADRKRILELALAEAGVKNCEIFGLPDFYNDAAWTQKMLEITG
                     LKAGETVLVSLNDWAKSCAKKIGIEAADQPVFCGNLSATQVRQTIFAGANWKNLVPPA
                     VFDCLQNEGLAQKIADCQIPPEDKIVEFVKRQMEAAGLERAVLGVSGGIDSAVMAAIL
                     GRALGKKATFVWMPFVRNCPFGKNVAKLESAFKFKAQKIYLDKVLQNFAKALPRGGNL
                     VYGNLKPRLRMAALYYFANLKKAMVIGTTNRTEFEIGYFTKYGDGAADMEPIADLFKS
                     EIYAMAERLKIPDDIMEVAPTAALWPGQTDEKELGLDYFRLDTALKLLAQGILPGEVC
                     SLANIDPKKMAKILARKEKNAHKLALPPILKLKQK"
BASE COUNT          890 a          644 c          839 g          792 t
ORIGIN      
        1 agggcatcgg caatctggcg gcgcgggtga ccaccgtggc aaaaaagatc ggcgcggcgg
       61 cgaacaaaga atatgcgaat gccgatttga aaaaagaagc cggaagcgcg cgggccgcca
      121 gagatgaatc tttggcgcaa tataaattta acgaggcgat tgccgcggtt tggaaattgg
      181 ttaattcggg cgacaggtat attgaacaaa cccagccgtg gaaaaccaaa gatgccgccg
      241 ccgcgggcga tttgctgttg ctgcttgccg actttgcgga tttgattgag cctttcgttc
      301 cgcaaacctc cgcaaagata aaaagcttgc ttgccggcga gaaccgcggc atccttttcc
      361 cgcggcttga cggcgaacaa tgaattttgt atatatttga atatggccga ggttatattc
      421 aaatataact ccttgaagat gagcaattta atcgcgttaa acaattttaa ctttttcttt
      481 agcccctctc gaagttcggg aattttttgt ttgcgcgatg aataaataaa atcatttatc
      541 aatccaaagg caagcggcaa attctcggac aacgagattt tttgttttta taacgcccgt
      601 tgtcccacag ttcgtttaaa attcggagga gataaaattg gtaaaagcag gagcaggatt
      661 aatagagcca tcttggaaga gaatggatgt cgacacaatt tttatcaggg gtattcataa
      721 tcccggaggt atcattacct tggaatggga cagggatatc caaggggcgg tatcgcaatt
      781 agggcttaat gccaattcgg ccagatttca agttgaatat ctggattcgc cgatgccaca
      841 acgggacgga tattatcgtc tggtggccat aaaaattatg gttggcggta gcggttccag
      901 cgtcatggat ttcttagaac aatttgccat gaatgaatgg cggcggttgg gcattaattt
      961 tgaaaacatt aacgttatcg agcggccgaa cagtgatagc cgtgtcgggt ttattatttg
     1021 ccggaaaaac gatttattgt tggtggccag cacatgggta ccagcctaat gggttgaaat
     1081 ctttcagccc ttttattttg attttggggc ttgacggatc tttatggatt tgatagacaa
     1141 taatcattaa gagattttat gaatcaaatc gcgcgggaaa ataattttgt attgttgccg
     1201 gtcctgggcc ttattattga ggagggcttt ttgtttggtg caagttaaag aaaataaaat
     1261 ttgttccaaa taaaaagccc ttccgcaaga agggttttta gttgggcggg ctaaccccaa
     1321 cggaaaatct gtctaggcag ggcttgcgag ttggcagacc gcttgagaaa acacaatact
     1381 caagcagagg ctcgttctcc aaagggacga aaggccaact cgcgtcataa tagttctttg
     1441 cgagggaata tgatggagga taaccgggaa aatatttttg gatatggagg gtaccaaccc
     1501 cctcctcaaa gaaataatga attgatggac aaagggcggg attctgatcg gcagcgcaaa
     1561 ttccccaatt aggtctgtga ggcaaaactt tcattgaaag tcttgcctca cagacatttt
     1621 ttttacgaag cgggcgtttt gttttgaaaa attgaaaaat ggaggggaat gggataaaat
     1681 caaaaaatac agataaagat aaagggtaaa attttacagg ggttcaaccc ctgttaaatt
     1741 atcaggggtt aaacccctgt aatttacccc tgcaatttgt tggaaaaata tggaaactac
     1801 tgactacaaa attcccaaag gcaaggaggt ggtgtttgtg gggcggttcc agccgcttca
     1861 tcgcgggcac ttggaagcga tcaaatggat tttggtgcaa tcaaagcgat tgaaaatcgt
     1921 tatcggcagt atgcaggaat tcgggcaggc cgacaacccg ctggaatttg ccgaccgcaa
     1981 aagaattttg gagctggcgc tggccgaagc gggggtgaaa aactgcgaga ttttcggatt
     2041 gccggatttt tacaatgatg cggcgtggac gcaaaaaatg ctggaaatca ccggtttgaa
     2101 ggccggcgaa acggtgctgg tgtcgttaaa cgattgggct aaatcgtgcg ccaaaaaaat
     2161 cggcattgag gccgccgatc agccggtgtt ttgcggcaac ctgtcggcca cgcaagtgcg
     2221 gcaaacgatc tttgccggcg cgaattggaa aaatttggtg ccgccggcag tgtttgactg
     2281 cttgcaaaac gaaggattgg cgcaaaaaat cgccgattgc caaattccgc cggaagacaa
     2341 gatcgttgaa tttgtgaaac gccaaatgga agccgccgga ttggaacgcg cggtgctggg
     2401 agtttcgggc ggcattgatt cggcggtgat ggccgcgatt ttgggccgcg cgctgggcaa
     2461 aaaggcaacc ttcgtttgga tgccgttcgt gcggaattgc ccgttcggaa aaaatgtcgc
     2521 caaactggag tcggcgttca aattcaaagc gcagaagatt tatttggata aagttttgca
     2581 aaacttcgcc aaggctcttc cccgtggcgg caatttggtt tatggcaatc taaagccgcg
     2641 tttgcgcatg gccgcgctct attattttgc caatctcaaa aaagcgatgg tgatcggcac
     2701 caccaaccgc acggaatttg aaatcggcta tttcaccaaa tacggcgacg gcgccgccga
     2761 catggagccg atcgcggatt tgttcaagtc cgaaatctat gcgatggccg aacggctcaa
     2821 aattcccgat gatattatgg aggtggcgcc cacggcggcg ctgtggcccg gccagaccga
     2881 cgaaaaagag ctgggcttgg attattttcg gctggacacg gcgctaaaat tgctggcgca
     2941 gggaatttta cccggcgagg tttgttctct tgccaacatt gatcccaaaa aaatggcaaa
     3001 gattttggcg cgcaaagaaa aaaacgccca caagcttgct ctgccgccga ttctaaaact
     3061 aaaacaaaag taaaccaaaa ttaaaatctt gaaactttgc atcaaatatt ttaaatgaag
     3121 caaaggtaat ccaccgcttt tagcggtgag accagaatag gttta
//
LOCUS       DAOQWE010000093         3157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_32561_length_3157_cov_4.186654, whole genome shotgun sequence.
ACCESSION   DAOQWE010000093 DAOQWE010000000
VERSION     DAOQWE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32561_length_3157_cov_4.186654"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PKK37_03385"
     CDS             complement(<1..382)
                     /locus_tag="PKK37_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR81454.1"
                     /translation="MKYIIVGSGFIFRRHLLSIKETGGEILDVVNETHGRGDEWKKAI
                     TNPDADCVVILAPNYLHYEIAKAANEAGKTVLCEKPLALKSEHVKDLADRKNIFVVQQ
                     LHYHEGVIKMRREISKDKHYDIHID"
     gene            complement(391..1194)
                     /locus_tag="PKK37_03390"
     CDS             complement(391..1194)
                     /locus_tag="PKK37_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81455.1"
                     /translation="MDKKIGIVGVGVVGNAVASVIPGVLLYDKYKSIGSVEEINEADI
                     IFVCVNTPYNPEKGCDISAVDEVISQIKGNKIIVIKSTVTPGTTERMQAKYPHHSVLF
                     NPEFLRQAHAADDMKNPDEQVVGYTAKSKQYAQEILDILPKSEHNFIVPSKDAEMAKY
                     FSNTFLALKVIFANQIYDVCQKMGIDYDAVKEVASVSPRFAFSHFDVNFDGYRGYSGA
                     CLPKDTKALIRFGDEIGVDLSLLKEADTINKKLLFSNKDISGDLLKLYQ"
     gene            complement(1209..2966)
                     /locus_tag="PKK37_03395"
     CDS             complement(1209..2966)
                     /locus_tag="PKK37_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNR81456.1"
                     /translation="MSNKKFFTGIILSSLLFFGLPFFVAADVVGEKRIFNVQSNLDAG
                     GRSQVAASLVHSGGKIYFYADDSWWEALNETEKNEKIAILKGIDAEFSNNVFPKLTQT
                     FGFTSNSGSLTVLFHEMIKNQRGYIRTGDNYSKDRVPSSNEREMIYLNQENLELSLPS
                     AALAHEYVHWITFNQKNLLRNVNDDVWSDEMRAVYSSTFLGYDVPFEGSVLEGELYVF
                     NNDSSESLVEWQNYLAQYAIAHLLAQYIVDHYGINVLADSMKTEKTGIASVDYGLANN
                     GFNIAFTQIFRDWLIALAVNNCSLGSKYCYKYQDLQKFFIEPQVSYFSGASRTMSFNT
                     SYFTPGWQKIIASEKNMRLEFSGDPQAKFIVPYLACDPANVCVAGELPVAADGSASLD
                     LPGLGGQYLSLTLMPFASGKTSGFRTEDGNGTNISSLSYSIKTSISTSVANGNGNGAE
                     IEPESEFQDVRIREMLAQIQLLKDEIIKIQTILAQKIGSAPAPASEYSCGAITENLYF
                     GCDNYAQVACLQEFLKNQGEAIYPQGLVTRRFSLDTKAAVIRFQEKYAAEVLWPLGLK
                     SGTGYVGAATRAKINQLMK"
BASE COUNT          856 a          749 c          660 g          892 t
ORIGIN      
        1 cgtcaatgtg aatatcataa tgtttatctt ttgatatttc acgcctcatt tttatcactc
       61 cctcgtgata atgcaactgc tggaccacaa aaatattttt tcggtcagcc aaatctttca
      121 cgtgttcgga ttttaacgcc agcggctttt cgcacaaaac ggtcttcccc gcttcattgg
      181 cggccttggc gatttcataa tgtaaataat tcggcgcaag aataaccacg caatccgcgt
      241 cgggattggt aatcgctttt ttccattcgt cgccccgacc gtgcgtttcg ttaacaacat
      301 ccaatatttc gccgccggtt tcctttattg aaagaaggtg ccgcctgaaa ataaaaccgg
      361 agcccacaat tatgtatttc atatatttcg ttattgatat aatttcagaa ggtcgccgga
      421 aatatcttta ttggaaaaca aaagtttttt attgatcgta tcagcttctt taagcaggga
      481 gaggtcaacc ccgatttcgt cgccaaatct aattaaagct ttggtatctt taggcaagca
      541 ggcgccgcta taacctctgt agccgtcaaa atttacgtca aaatgagaaa acgcgaatct
      601 cggactgacc gatgccactt ctttcaccgc atcataatca atccccattt tctggcatac
      661 atcataaatc tggttggcaa aaataacttt aagcgctaaa aaggtgtttg aaaaatattt
      721 tgccatttcc gcgtctttgg acggaacaat aaagttatgc tccgatttgg gcagaatatc
      781 caaaatttct tgcgcgtatt gcttgctttt tgcggtatac ccgacaactt gctcatcggg
      841 atttttcatg tcatcagcag catgcgcttg gcgcaaaaat tcaggattga acaatacgga
      901 gtggtgtgga tactttgctt gcattctttc ggttgttccg ggagtgacgg ttgattttat
      961 gactatgatt ttgtttcctt tgatttgaga aataacctca tccaccgcgg agatatcgca
     1021 tcctttttcg gggttataag gagtattaac gcacacgaaa ataatatcgg cttcattaat
     1081 ttcctcaaca gaaccgatgc ttttgtattt atcatagagc aacacgcccg gaataacact
     1141 ggcaaccgca ttaccaacaa cacccactcc gacaatgccg atttttttat ccataatact
     1201 tttaattgtt atttcattag ctggttgatt ttggcgcggg tggcggcgcc gacgtagccg
     1261 gtgccggatt tcaaccccag cggccaaaga acttcggcgg catatttttc ctggaagcgg
     1321 atcaccgcgg ctttcgtatc cagggaaaaa cggcgggtaa ccaatccctg cgggtagatg
     1381 gcttcaccct gatttttcaa aaattcctgc aagcacgcaa cttgcgcata attgtcgcaa
     1441 ccaaaatata aattttcggt aatcgcgccg caagaatatt cagaagccgg cgcgggcgca
     1501 ctgccgattt tttgcgccaa tattgtttga attttgataa tttcatcttt gagcagttga
     1561 atttgcgcca gcatttcccg aatccttacg tcttgaaatt cgctttccgg ctcaatttcc
     1621 gcgccgttgc cgttgccatt ggcaacggac gttgaaattg aagttttaat ggaatatgaa
     1681 agcgaactga tattggtgcc gttgccgtct tcggttctaa accccgatgt ttttccggaa
     1741 gcaaacggca tcagagtaag cgacaaatat tgcccgccga gccccggcaa atccaaactc
     1801 gcgctcccat cagcggccac cggcaattct ccggcgacgc agacattcgc cgggtcgcag
     1861 gccaaataag gcacgatgaa tttcgcttgc ggatctcccg aaaactccag ccgcatattt
     1921 ttttccgatg cgataatctt ttgccatccg ggagtgaaat atgaggtgtt aaaagacatt
     1981 gtccggcttg ctcccgaaaa ataagaaact tgcggttcaa taaagaattt ttgcaaatct
     2041 tgatatttat aacagtattt tgaacccagc gaacaattat taacggccag cgcaatcaac
     2101 cagtcacgaa aaatctgcgt aaaagcgata ttgaagccgt tgttcgccaa tccgtaatca
     2161 acggaggcaa tgccggtctt ttcggttttc atggaatcag ccaaaacgtt aatgccgtag
     2221 tgatcaacaa tgtattgcgc caacagatgt gcgattgcgt attgcgccaa ataattttgc
     2281 cattccacca agctctccga agagtcattg ttgaaaacat aaagttctcc ttccagcaca
     2341 ctgccttcaa acggaacatc atatcccaaa aaagttgacg agtaaaccgc gcgcatttcg
     2401 tcgctccaaa catcatcgtt gacattacgc aaaagatttt tctggttaaa ggttatccaa
     2461 tgcacatatt catgggctaa agccgccgaa ggcaacgata attcaaggtt ttcttgattc
     2521 aaatagatca tttccctctc attggacgaa ggaactcgat cttttgagta gttatccccg
     2581 gtccggatat agccacgctg gtttttgatc atttcatgaa acagcacagt taaactgccg
     2641 gaatttgaag taaacccgaa agtttgcgtt aatttgggaa agacattgtt tgaaaactcg
     2701 gcgtcaatac cttttaaaat ggcgatcttt tcgttttttt ccgtttcgtt tagtgcttcc
     2761 caccacgaat cgtcggcata aaaataaatc ttgccgcccg aatggaccaa ggacgccgcc
     2821 acctggcttc gcccgccggc atcaagattg gactggacat tgaaaattct tttttctccg
     2881 acaacatcag ccgcgacaaa aaacggtaac ccaaaaaaaa ggaggctgga taatatgata
     2941 ccggtaaaaa attttttgtt ggacatatcc gtttttatgg ttcaaaaaaa catcccctat
     3001 tacatcaagg cattgatttt ggcgcgggtg gcggcgccga cgtagccggt gccggatttc
     3061 aaccccagcg gccaaagaac ttcggcggca tatttttcct gaaagcggat caccgcggcg
     3121 cgggtggcgg cgtcaaaatt gccgctcaaa agtccgc
//
LOCUS       DAOQWE010000094         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_32570_length_3156_cov_7.138665, whole genome shotgun sequence.
ACCESSION   DAOQWE010000094 DAOQWE010000000
VERSION     DAOQWE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32570_length_3156_cov_7.138665"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="PKK37_03400"
     CDS             complement(<1..1802)
                     /locus_tag="PKK37_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNR81457.1"
                     /translation="MINQPPNFTHKAQEAILYAQDLGREKGQQQVDALHLLAALLSQE
                     KSLILNLLERLSVDVQNLRKRTMGALSRLPVIAAPQAFGQFYLTQDMAKVLERAREEA
                     SKFGDEFISVEHLFLALAGTATKAREVLDKVEFIRADGAFAAPEIGVLDYETVLKELA
                     KVRGGQRVTDPEPESKYQVIEKYTRNLTTMARQGKLDPVIGRENEIRRLMQVLSRRTK
                     NNPVLIGEAGVGKTAIVEGLVQRIASGNAPESLRDKEIISLDLGALIAGTKYRGEFED
                     RIKALLREVERAAGRYIFFIDELHTLVGAGAADGTIDASNLLKPVLARGERKAIGATT
                     LKEYQKYIEKDPALERRFQPVFVAEPSMEDAKMILLGLKEKYEMHHGVKIDERGLAAC
                     VELASRYIPDRFLPDKAVDLMDEAMSALRLDIESEPEEVEKLRKELQHLQVEYEAIKK
                     DRGQEIILRRKAIERQMADLGEKLQEIEAKWRSEKETIGKIKNLKKELEASRFEVEKE
                     EAQANLQRVAELKYGRIPQLIKELKSAEKKLARFQKSRPILKEQITEEDVANVVSRWT
                     GIPVTRLMEEEARKLQNLEEILKRRVIGQEAAIGA"
     assembly_gap    1823..1922
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1923..>3156)
                     /locus_tag="PKK37_03405"
     CDS             complement(<1923..>3156)
                     /locus_tag="PKK37_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR81458.1"
                     /translation="MSMYDLTSKEESKSNFLGMGKDRKKNIWPVMLALAAGFALGALA
                     FSQDFDSLAERLNLPAMKFAPQSVETPSADVPAEYVPQTSQEEAVVKAVKDYSSAVVS
                     IIITKDMPVYERYYTRGTDPFDFWDFQIPQLRQRGTQERQIGAGTGFIVDEDGTILTN
                     KHVVSDDQAQYTVVMSDGERYSAKVTAADPVNDIAVVKISDGGPRRFPAAVLGDSDNL
                     QIGQSVIAIGNTLGEFDNTVSVGVISGMQRSITAYGAGEDEVLEDLIQTDAAINEGNS
                     GGPLLNLKGEVIGINTAIASGAQNVGFVIPINLAKRGLAQAKTSGKIVYPYLGVYYTM
                     IDARLQKENSLPADYGAWITADGGSGAAVIAGSPAQAAGLKDGDVILEISGQKLDQKN
                     SLARVIAKYNPGDKVKLKV"
BASE COUNT          619 a          944 c          725 g          768 t
ORIGIN      
        1 gcgccaatcg ccgcctcctg cccgatcacc cggcgcttta aaatctcttc caaattttgc
       61 agtttgcgcg cctcttcttc catcagccgc gtcaccggaa tgccggtcca gcgcgacacc
      121 acgttggcaa cgtcttcttc ggtaatctgc tccttgagaa tcgggcggct tttttgaaac
      181 cgcgccaatt ttttttccgc ggacttgagc tctttgatca gttggggaat tcggccgtat
      241 ttcaattcgg ccacccgctg aagattcgct tgcgcctctt ccttttccac ctcaaaacgg
      301 ctcgcttcaa gctctttttt cagattcttg attttgccga tcgtttcttt ttcggaacgc
      361 catttggcct caatttcctg caatttttcg cccagatcgg ccatttgccg ttcaatggct
      421 ttgcgccgga ggataatttc ctggccgcgg tcttttttaa tcgcttcgta ttccacctgc
      481 aaatgctgga gttctttgcg taatttttcc acttcctccg gctcggattc aatgtccagc
      541 cgcagggcgc tcatcgcttc gtccatcaag tccaccgcct tgtcgggcag gaatcggtcg
      601 ggaatgtagc gcgacgccaa ttccacgcat gccgccagtc cccgctcgtc aattttgacg
      661 ccgtgatgca tttcatattt ttctttcagt cccagcagga tcattttggc atcttccatt
      721 gacggctcgg ccacaaacac cggctggaag cgccgttcca gcgcggggtc tttttcaatg
      781 tatttttgat actctttcaa cgtggtggcg ccgatcgctt tcctttcgcc gcgcgccagc
      841 acgggtttga gcagattgga cgcatcaatc gtgccatcgg cggcaccggc gcccaccaac
      901 gtgtgcaatt cgtcaataaa aaagatatac cggccggcgg cgcgctccac ttcgcgcaat
      961 aacgccttga tgcggtcctc aaattcgccg cggtatttcg tgccggcgat cagcgcgccc
     1021 agatcaagag aaataatttc tttgtcgcgc aggctttcgg gcgcattgcc cgaggcgatg
     1081 cgctggacca aaccctccac gatcgcggtc ttgcccacgc ccgcctcgcc gatcaacacc
     1141 ggattgttct tggtgcggcg gctcaaaacc tgcatcagcc ggcgaatttc gttttcccgg
     1201 ccgattaccg gatccaattt tccctgtcgg gccatcgtgg tcaaattgcg ggtgtatttt
     1261 tcaatcactt gatatttgga ttccggttcc ggatcggtca cgcgctggcc gccgcgaact
     1321 ttggccaatt ccttcaaaac ggtttcataa tcaagcacgc cgatctcggg cgcggcaaaa
     1381 gcgccatccg cgcggatgaa ttccactttg tccaatactt cccgcgcttt ggtggcggtg
     1441 ccggccagcg ccaaaaacag atgctccacc gaaatgaact catcgccgaa tttggacgct
     1501 tcttcccgcg cccgctccag cactttggcc atatcctgtg tcagatagaa ctggccgaac
     1561 gcctgcggcg cggcgatcac cggcaaacgg gaaagcgctc ccattgtccg cttgcgcaaa
     1621 ttttgcacat caacgctcaa gcgttccagt aaattcaaaa tcaggctctt ttcctgggac
     1681 aagagcgccg ccagcaagtg cagggcgtcc acctgttgct ggcccttttc ccgccccaga
     1741 tcctgggcgt agagaatcgc ttcctgcgcc ttatgggtaa agtttggtgg ttgattgatc
     1801 ataataaatt taaaattcaa aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnggactttc agtttcacct tatcacccgg attatatttt gcaataaccc gcgccaagga
     1981 atttttttgg tccagctttt ggccgctaat ctccaagatg acatccccgt ctttcaatcc
     2041 ggcggcttgc gcgggagaac cggcgatcac cgccgcgccg gagccgccgt cagcggtaat
     2101 ccacgcgccg taatccgccg gcagactgtt ttctttttgg aggcgcgcgt caatcatcgt
     2161 gtaataaaca cccaagtaag gatagacgat ctttcccgaa gttttggcct gagccaatcc
     2221 gcgcttggcc aaattgatcg gaatcacaaa tcccacattt tgagcgccgc tggcaatcgc
     2281 ggtgttaatg ccgatcacct cgcctttcag atttaacagc gggccgccgg aatttccctc
     2341 gttgatggcc gcgtcggttt ggattaaatc ttccaaaact tcgtcctcgc ccgcgccgta
     2401 ggcggtaatt gacctctgca tccccgaaat cacgcccacc gacaccgtat tgtcaaattc
     2461 tcccaaggta ttgccgatgg caatcacgct ttgcccaatc tgcaaattgt cggaatctcc
     2521 cagaaccgcc gcgggaaacc gccgcgggcc gccatcggaa atcttcacca ccgcaatgtc
     2581 attgaccgga tcggccgccg tcactttggc ggaataacgc tcgccatcgg acatcaccac
     2641 ggtatattgc gcctgatcat cgctcaccac gtgtttgttg gtcaggatgg tgccgtcttc
     2701 gtccacaatg aagcccgtgc cggcgccaat ctgccgttcc tgcgtgcccc gctggcgcaa
     2761 ttgcggaatc tgaaaatccc aaaagtcaaa aggatcggtc ccgcgcgtat agtatctttc
     2821 ataaaccggc atatccttgg tgataatgat ggacaccacc gccgaagaat aatccttgac
     2881 cgccttgacc accgcttctt cctgcgaagt ctgcggcaca tattccgccg gcacgtccgc
     2941 ggacggcgtc tcaacacttt gcggcgcgaa tttcatcgcc ggcaagttaa gccgctcggc
     3001 caaagaatca aaatcttgcg aaaacgccag cgcgcccaga gcaaaccccg cggccagcgc
     3061 caacatcacc ggccaaatgt tttttttgcg atccttgccc attcccaaaa aattgctttt
     3121 tgactcctct tttgaggtga gatcatacat tgacat
//
LOCUS       DAOQWE010000095         3147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_32704_length_3147_cov_9.087969, whole genome shotgun sequence.
ACCESSION   DAOQWE010000095 DAOQWE010000000
VERSION     DAOQWE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32704_length_3147_cov_9.087969"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKK37_03410"
     CDS             complement(<1..192)
                     /locus_tag="PKK37_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81459.1"
                     /translation="MDKKIADKLLAQTRENYDDFAASFSQTRSYIPENIAALLKDFAR
                     QGDRVLDIGCGNGRLCPFFE"
     gene            complement(224..796)
                     /gene="idi"
                     /locus_tag="PKK37_03415"
     CDS             complement(224..796)
                     /gene="idi"
                     /locus_tag="PKK37_03415"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNR81460.1"
                     /translation="MEDDILLVDEDNNPVGAGEKMDVHRRGVLHRAFSILVYNSKKEM
                     LLQRRAAAKYHCPGQWSNACCSHPAPGDHLLLAARRRLKEEMGISIPVKETGVEFIYK
                     VKVGDLTEYEYDHLLYGQFDGEPKINPQEADDWKWMAFADLRKDMKRNPEFYTPWFRL
                     IASQGDNVVEQIPVARLEKYEIKRLATYSN"
     gene            complement(932..1153)
                     /locus_tag="PKK37_03420"
     CDS             complement(932..1153)
                     /locus_tag="PKK37_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNR81461.1"
                     /translation="MLIKVHVYPCSKEEQVGQIGADAFKVNVAAKPENGQANARAREL
                     LAHYFAVPAGKVHLIKGAREPHKIFEIIE"
     gene            complement(1207..2226)
                     /gene="rlmN"
                     /locus_tag="PKK37_03425"
     CDS             complement(1207..2226)
                     /gene="rlmN"
                     /locus_tag="PKK37_03425"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNR81462.1"
                     /translation="MDLSKLNEILADQPAYRAGQIKEAVYKLSVEDWDEAIGLPRGLR
                     QQLKEKCPLNINAEFFVSGGGNSQKAVVRLTDGLKIEAVLMCHDNRNTVCVSSQAGCP
                     MACSFCATGKMGFKRNLAVGEIVEQVLLFVRALKKEGQRVTNVVFMGMGEPFLNYDNV
                     MAAIKVLNDQKGLNIGGRHISISTCGVIEGIEKLIDEPLQVNLAVSLHAPNNRLRSRL
                     MPVNNKYPINDIMKSVLRYVKNTNRRVMFEYIMIDGINDSEDCARELAELIRSRLPLK
                     LAFVNLIYYNPTGIYNTSLPKRVKMFKTILMQAGVEATERYRFGREINAACGQLATEA
                     KSEKE"
     gene            complement(2217..2744)
                     /locus_tag="PKK37_03430"
     CDS             complement(2217..2744)
                     /locus_tag="PKK37_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR81463.1"
                     /translation="MKIEKIVVGMLRANCYLLVDGGEAAVIDPGDEPQKIIDAIEKSG
                     AKLKFIANTHRHFDHIGANAAVAEKFGVEVLANLNDGDILAIGKSKLKVVKTPGHTPD
                     GVCFFGDGFVVSGDTLFEDGIGRTDLEGGSDEQMTASLKKLDALIPDSAIIYPGHGNA
                     FTYKKGIALEYLKWI"
     gene            complement(2881..>3147)
                     /locus_tag="PKK37_03435"
     CDS             complement(2881..>3147)
                     /locus_tag="PKK37_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNR81464.1"
                     /translation="NMFEKLKQIKKLQEIQGALKNEKVENEKNGVKILMNGKLEVEDV
                     QLNPDLSLAEQEKAVRDCINDAMKKVQMVVARKMQSMGMGGFGM"
BASE COUNT          799 a          846 c          617 g          885 t
ORIGIN      
        1 ctcaaaaaac ggacacaggc ggccattgcc gcaaccgatg tccagcaccc tgtccccctg
       61 ccgcgcgaag tctttcaata atgcggcgat attttcggga atgtagctgc gggtttgcga
      121 aaaagacgcg gcaaaatcat cataattttc gcgggtttgc gccaacaatt tatcggcaat
      181 ttttttatcc acgattaaaa atcaaaaacc gaaactctac cttttaattt gaataggttg
      241 ccaatctctt gatttcgtat ttttccagcc gcgccaccgg aatttgctca accacattat
      301 ccccttggct cgcaatcagg cgaaaccagg gagtgtagaa ttccggattt cgtttcatat
      361 ctttgcgcaa atcggcaaac gccatccatt tccaatcatc ggcctcttgc ggattgatct
      421 tgggctcgcc gtcaaattgt ccatataaca gatggtcata ttcgtattcg gtcagatcgc
      481 ccactttcac tttatagata aattccacgc cggtttcttt gacggggatg gaaatcccca
      541 tctcttcttt cagacgccgc cgcgccgcca acagcagatg atcgcccggc gccggatgcg
      601 aacaacaggc attgctccac tggccggggc aatggtattt ggccgcggcg cggcgctgta
      661 gcaacatttc ttttttggaa ttatagacca agattgaaaa cgcgcggtgc aaaaccccgc
      721 gccggtggac atccatcttt tcaccggcgc cgacaggatt gttgtcttcg tcaaccaaga
      781 gaatatcatc ttccataatt tcaaatatca aagctcaaat gccaaacaaa atccaaagct
      841 caaatatcaa agcaggcgct acttcgctct ttgccgtttg gttttcgggc tttgagcttt
      901 gtttgacatt tattatttga actttgatat tttattcaat gatttcaaat atcttgtgcg
      961 gttcgcgcgc acctttgatc aagtgcactt tacccgccgg cacggcaaaa taatgcgcca
     1021 acaactcccg ggcgcgcgca ttggcttggc cgttttccgg cttagccgcc acattcactt
     1081 taaacgcatc cgctccgatt tggcccactt gctcttcttt ggagcacgga taaacatgaa
     1141 ccttaattaa catattaaaa gtttaaagct aaaagtgaag atttaaaagt cagcctaatc
     1201 ccggaattat tccttttcgc ttttcgcttc cgtggccagc tgtccgcagg ccgcattaat
     1261 ttcccggcca aaacgatagc gttcggtcgc ttccactccg gcttgcatca aaatcgtttt
     1321 aaacatcttg acgcgcttcg gcaaagacgt attgtagatt ccggtcggat tgtaataaat
     1381 aagattaaca aacgccagct tgagcggcaa acgcgacctt atcaattccg ccaactcgcg
     1441 ggcgcagtct tcggaatcgt tgatgccgtc aatcataata tattcaaaca ttacccgccg
     1501 gttggtattt tttacataac gtaaaaccga tttcattata tcattgatcg ggtatttgtt
     1561 gttcaccggc ataagtctgg atcgcaatct attatttggc gcgtgcaatg acaccgccaa
     1621 attaacttga agcggttcat caatcaactt ctcaattcct tcaataacac cacaggtgga
     1681 tattgaaata tggcggccgc cgatattcaa gcccttttga tcgtttagaa cctttatcgc
     1741 cgccatcaca ttatcataat tcagaaacgg ttctcccatc cccatgaaca ccacattagt
     1801 cacccgctgg ccttcttttt tcaaggcgcg gacaaacaat aacacctgct ccacaatctc
     1861 gcccaccgca agatttcttt taaatcccat ttttccggta gcacaaaatg aacaagccat
     1921 tgggcaaccg gcctgagaag atacgcaaac cgtattgcga ttatcgtggc acatcaaaac
     1981 cgcttctatt tttaatccgt cagttagtct cacgacagct ttttgagaat tgccgccacc
     2041 ggaaacaaaa aactcggcat tgatattaag cggacatttt tcttttaatt gttggcgcaa
     2101 tccccgcggc aatccgatag cctcgtccca atcctccacc gataacttat aaaccgcttc
     2161 tttgatctgt ccggcccgat aagccggctg atcggccaaa atttcattca atttacttaa
     2221 atccatttca aatattcaag ggctatgccc tttttatagg tgaaggcatt gccgtggccg
     2281 ggatagatta tggcgctgtc gggaattaat gcgtcaagtt ttttcagcga cgcggtcatc
     2341 tgttcgtcgg atccgccttc aagatcggtg cgcccaatgc cgtcttcaaa aagagtgtcg
     2401 cccgaaacca caaaaccatc gccaaaaaag cagacgccgt cgggcgtatg cccgggggtt
     2461 ttaacaactt ttaattttga tttgccgatt gccaaaatat ctccatcatt cagatttgcc
     2521 aaaacctcaa cgccgaattt ttcggcaacc gcggcattgg cgccgatatg gtcaaaatgg
     2581 cgatgagtgt tggcaataaa tttcaatttg gcaccgcttt tttcaatcgc atcaataatt
     2641 ttttgcggtt cgtcgcccgg atcaatcacc gccgcctctc ccccgtcaac cagcagataa
     2701 caattcgctc gcaacatccc gaccacgatt ttttctatct tcataaaaaa taaaattgca
     2761 aaccgcgttg aggcaagcat tttttgcgat gccgcaccgg cgacggccgc gcgaaagcaa
     2821 tttgccagca ggcaaatatg cgcgtcgcaa aaaatgcttg ccgaataagc gttgctaatt
     2881 ttacatccca aaccctccca ttcccattga ctgcattttt ctggccacca ccatctgcac
     2941 ttttttcatc gcatcattga tgcaatcccg aaccgccttt tcctgctccg ccagggaaag
     3001 atcggggttc agctggacat cttccacttc caatttgccg ttcatcagga ttttaacgcc
     3061 gtttttttcg ttttccactt tttcgttttt cagcgcgccc tgtatctcct gcaacttttt
     3121 gatctgtttc aatttctcaa acatatt
//
LOCUS       DAOQWE010000096         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_32729_length_3146_cov_7.063410, whole genome shotgun sequence.
ACCESSION   DAOQWE010000096 DAOQWE010000000
VERSION     DAOQWE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32729_length_3146_cov_7.063410"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1031)
                     /locus_tag="PKK37_03440"
     CDS             complement(138..1031)
                     /locus_tag="PKK37_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNR81465.1"
                     /translation="MVERLNDRKKEERLMESLQGLNRDMYRPLKIINKGKTKEIWATN
                     VPGIVILRATDDITAGDGAKHDTFESKARYANQTTCNVFDLLKTCNVPLAYIGQYDET
                     SFAAEQCDMIPLEVVVRREAYGSYLKRNPEVGKGTIFFENPVVEFYLKTTERKWQGKT
                     IPVDDPFIVFGRNKMLLYRPDQPIERQEPFLALEDYPLCGWPEAYDRIARIAIGVFII
                     LEKVWGPHYRMVDYKVEFGINKHEEIVLADVIDNDSWRVLDEFGEHMDKQIYREGGDL
                     ASVADRYRKVAELTDKFPQLR"
     gene            complement(1053..2444)
                     /locus_tag="PKK37_03445"
     CDS             complement(1053..2444)
                     /locus_tag="PKK37_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNR81466.1"
                     /translation="MEIAAVLAEFGKDDERVKRLIEALKNSPPNEERRKEIERVTDHD
                     LMAYVMQRWEILSPELQAIFHKAKTSFDIQDPADQLALITALDVVIDDLRSLMEITRN
                     QGLKYWYSPLLLRTHGQGAHVGTFGKRLSSYFADLEFAQNYLLYARKMISFGKMSGLV
                     GDYQNASYQIEYQALKMLELKPYYGASQILPRSMHAILASSLTVLCGVLAKIAEDFQL
                     MARNPRPLIQEPFKSGQTGSSASPAKKNSIKSENTEGMFRLALGDLVALLQNLITKEE
                     RDIAQSSVERVALIDLFHISLNALKNVSYVMENMAVYPVHMLDEILEMCGTEAAEAAK
                     EFLVENGIPRETAYAIVKQASHNAFNFAKIDCLDIPTSPQEAELFYRDYAQKLHEKPD
                     NIRDIIAQGRLFYLPTVNVGREAIKDWNTLLRGIFADQNVYAKWEKVFDIEHIFASQE
                     VLLEKMREGAIKV"
     gene            complement(2744..3010)
                     /locus_tag="PKK37_03450"
     CDS             complement(2744..3010)
                     /locus_tag="PKK37_03450"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNR81467.1"
                     /translation="MKTKPSKKSFRVKVFEIVKKIPKGKTLTYKTVAEKAGSPKAYRA
                     VGNVLHTNYDSAIPCHRVVRSDGNPGGWNRGKQKKAELLKKEKL"
BASE COUNT          811 a          798 c          564 g          973 t
ORIGIN      
        1 acccccccga gtgataacgc gctttgaagc gaccgcccag cgagatgtgg ctttaattaa
       61 acttttattt tatttttccc ttaatttatc gcaaatataa attaagattg tataaaaaaa
      121 caggatcatt gaccctgtca tcgcaattgc gggaatttat ccgttaattc tgcaactttt
      181 cggtatctat ctgcgacgct cgccaaatca cctccttcgc ggtagatctg cttgtccatg
      241 tgttctccaa actcgtccaa aacgcgccat gaatcattgt caataacatc agcaaggaca
      301 atctcctcat gtttattgat tccgaattca actttgtaat caaccatccg atagtgaggc
      361 ccccatactt tctccaaaat tatgaaaact ccaatagcaa ttcgtgctat cctatcatat
      421 gcctcaggcc acccgcacag tggatagtct tcaagagcca aaaatggttc ttgcctttct
      481 atcggctgat ccggacgata tagtagcatt ttatttcttc cgaaaactat aaacggatcg
      541 tccaccggaa tagtttttcc ttgccatttt ctctccgtgg tcttgagata aaactccacc
      601 acaggatttt caaagaaaat agttcccttt cctacttccg ggtttctttt cagatatgat
      661 ccgtaagcct ccctcctgac caccacttcc agaggaatca tgtcgcattg ttcagccgca
      721 aaacttgttt catcatattg gccaatatat gccagcggaa cattgcacgt tttcagtaaa
      781 tcaaacacat tgcaagttgt ttgattcgca taccgcgcct tgctctcaaa agtatcgtgc
      841 ttagcgccat cgccagccgt gatatcatct gtcgctctca gaatcactat cccaggaaca
      901 tttgttgccc aaatctcctt agttttccct ttgttaataa ttttaagggg tctatacata
      961 tctctattca aaccttgtaa agattccatt aatctctcct ccttttttct atcgttcaac
     1021 ctctcaacca aaaatttagc aaggtttatg cgctaaactt ttattgctcc ttctctcatt
     1081 ttttccaaca atacctcctg gcttgcgaat atatgctcaa tatcaaacac tttttcccat
     1141 ttggcatata cattctggtc agcgaatatc cctcgcaaca gagtattcca atccttgatt
     1201 gcttccctgc caacattaac agttggcaaa tagaacaatc tgccttgtgc aatgatatct
     1261 ctgatgttat cgggcttctc gtgcagcttc tgcgcataat cgcgatagaa taattccgct
     1321 tcttgaggac ttgttggaat gtccaaacaa tcaattttgg caaaattgaa tgcgttatgc
     1381 gacgcttgtt tgacaatcgc ataagcggtt tctcgcggaa ttccgttttc cactaaaaac
     1441 tcttttgccg cctccgccgc ttccgtgcca cacatttcca gaatttcatc cagcatatgg
     1501 acaggataca ctgccatatt ctccataaca taggacacat tctttaatgc gttcaatgat
     1561 atatggaaca agtctatcag cgccacccgc tccaccgacg attgggcaat atcgcgttct
     1621 tctttagtga taaggttttg caacaatgcc acaagatcgc caagtgcaag acgaaacatt
     1681 ccttccgtgt tttcactttt gatggaattc ttcttggccg gacttgcact tgatccggtt
     1741 tgtccactct tgaagggctc ttgaatcagc ggcctgggat ttctggccat cagctgaaaa
     1801 tcttccgcta tcttagcaag tactccgcaa agcacagtaa gtgaagaggc taaaatcgcg
     1861 tgcattgacc ttggcaatat ttggctcgct ccgtaatatg gcttcaattc caacattttc
     1921 aatgcctgat actctatctg atatgacgca ttttggtaat cacccacaag tcccgacatt
     1981 ttgccaaaac tgatcatttt gcgcgcatat aaaagataat tttgcgcaaa ctccagatcg
     2041 gcaaaatatg acgacaacct cttgccaaaa gtaccgacat gggctccttg cccatgagtt
     2101 cttagcaata agggagaata ccaatatttt agcccctgat tacgggttat ctccatcagg
     2161 cttcgcaaat cgtctatcac cacatcaagc gcggtgataa gcgccaattg gtcggcggga
     2221 tcttgaatgt caaagctcgt tttagctttg tgaaatatag cctgcaattc tggcgacaat
     2281 atctcccagc gttgcattac ataggccatt agatcatgat ctgtcaccct ttctatttcc
     2341 ttgcgcctct cctcattggg cggactattt ttcaaagcct cgataagtct cttgacgcgc
     2401 tcatcatctt tgccaaattc ggccagaact gccgcaattt ccacttggtc aaacctggac
     2461 agcctttgtt gcggagaaaa aatctcttct atcctcggat ccctatatct tgatatcgta
     2521 tttaccaccc catcttcgtt ttctctcttg ctcacccatc gcgccttgcc aaagtttcat
     2581 attcagttga caaggtgcga attgagcttg cgacacaaaa aatcgcgcaa aaagctcgtt
     2641 ttgagattaa aatggatttc cctttccgtc aagcgtttca acagtaatta cggacattcc
     2701 aacattcttg agaatgtgag aatgtccgat aatagtttgg aaattaaagt ttttcttttt
     2761 tcagcaattc cgctttcttt tgcttccctc ggttccatcc tcccggattt ccgtccgagc
     2821 gcacgacgcg atggcaaggt atggcggaat cgtaattggt gtgcaacacg ttgcccaccg
     2881 cgcgataagc tttcgggctt ccggcttttt cggccaccgt tttataagtt aaagtttttc
     2941 ctttgggaat ttttttgacg atttcaaaaa ctttgacgcg aaaagatttt ttggagggtt
     3001 tcgttttcat atgttatggt atcattccaa aattcccggc gcaatcggaa ccgccgcgga
     3061 aaatgcaatc ataagtggca tcgccaaaca acccaaaaag ctcccttggc gggagctttt
     3121 tgatttcaat caaccggatt tctaca
//
LOCUS       DAOQWE010000097         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_32886_length_3135_cov_8.094481, whole genome shotgun sequence.
ACCESSION   DAOQWE010000097 DAOQWE010000000
VERSION     DAOQWE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32886_length_3135_cov_8.094481"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKK37_03455"
     CDS             <1..381
                     /locus_tag="PKK37_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81468.1"
                     /translation="LAPKIFNEFIARKNIKLTELRCAIIPQGLVLPKNEKITLCGDAS
                     GLTKPWSGGGVIWNLTQAGILLKNFPDFLQYRKDATRFFNLRIMAGKAAKFCAYTAGF
                     HFPWIIPQNINLDGDYLLKFNRKI"
     gene            390..1076
                     /gene="uppS"
                     /locus_tag="PKK37_03460"
     CDS             390..1076
                     /gene="uppS"
                     /locus_tag="PKK37_03460"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNR81469.1"
                     /translation="MENQKNLPRHLGIILDGNRRWAKERGLPTLEGHHKGFENVKLII
                     QAAQDRGIETLTLFCFSTENWHRSKEEVDYLMDIFRKSLSDYFGKFNEKNVKINVLGQ
                     KEKFAGDIKEQIEKIESETKNNTGMTLNLALSYGGRAELVDAIKKIVKKGIDPDKITE
                     DTVKDNLWTSDADLIIRTGGEQRLSGFLLWQAAYSEFLFVKKYWPDFGENDLDAALAE
                     YASRQRRFGK"
     gene            1143..1925
                     /locus_tag="PKK37_03465"
     CDS             1143..1925
                     /locus_tag="PKK37_03465"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNR81470.1"
                     /translation="MSLTIIKIGGSVITKKESKKPKINAGNINKLGSLLKGFKESYVL
                     VHGVGSFGHPLVKQTGIDKSVISEKQILAFAKTQKLQNDLNCMVCNRLIKKGVPAFPA
                     QASSHAVLERGRLVSMATEAMGGLLRLKMVPVAYGVPAFDKAWGSAILSGDQITPYIA
                     KKMGAEKIIEVSDVEGIFTANPKINKNARLIPEINRKNYQDIQKCLSGSTAADVTGGM
                     KQKYLELLDAAKTGIICQIVDLKHFKNALEGKPAGTVINLQQ"
     gene            1925..2266
                     /locus_tag="PKK37_03470"
     CDS             1925..2266
                     /locus_tag="PKK37_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006869741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR81471.1"
                     /translation="MKIVKKDQRKEFKNSDCCLAYEYPMDDCDINGAVIKLSGRYPDK
                     GWAANEVCKEMGYIISGSGKLVVEDKEYELTAGDLVLINPGEKFYWQGNMEIFMPCAP
                     AWSPDQYKTVK"
     gene            2385..2840
                     /locus_tag="PKK37_03475"
     CDS             2385..2840
                     /locus_tag="PKK37_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81472.1"
                     /translation="MQIQDAKEKIREAVREFKALDRAALLKVNVYEPAISHRIAVYLG
                     NLFRELNVDCEYNRNLLTAKKNTNGDKIRPDIIIHRRLSPNNCIIFEIKKGGKDSQKA
                     ITDIRKLEDAVAGNLGYDLGVFIGILKRRIDICWIEKINSTLFKTCETI"
     gene            2941..>3135
                     /locus_tag="PKK37_03480"
     CDS             2941..>3135
                     /locus_tag="PKK37_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81473.1"
                     /translation="MNLSGILILIALVFGAYAALILLRKKRLSDLLQKLAKYDRDFKV
                     AIIDFHKLFQNNWYISDWQYQ"
BASE COUNT         1017 a          628 c          698 g          792 t
ORIGIN      
        1 ttggcgccga aaatttttaa tgaatttatc gcacgaaaaa atatcaaatt aacggaattg
       61 cgttgcgcga tcattccaca aggattggtt ttgccgaaaa acgaaaaaat aactttgtgt
      121 ggcgatgcca gcggactgac caagccgtgg tcggggggcg gcgtgatttg gaatttgacg
      181 caagccggca tcctcttgaa aaatttcccc gatttcctac aatacaggaa agacgctacg
      241 cgctttttta atttgcggat aatggcaggg aaagcggcca aattttgcgc ctataccgcc
      301 ggatttcatt ttccatggat tattccgcaa aacatcaatc ttgacggcga ttatctgctg
      361 aaattcaatc gaaaaattta attttgataa tggaaaacca aaaaaatctg ccccgtcatt
      421 tgggaattat ccttgacggc aaccgccgct gggccaagga gcgcggcctg ccaacactgg
      481 aaggccacca caaaggcttt gaaaatgtta aactaatcat acaagcggcc caagacagag
      541 gaattgaaac actcacgctt ttttgttttt caactgaaaa ttggcatcgt tccaaggagg
      601 aagttgatta tttgatggat attttcagaa aatcattatc ggattatttt ggtaaattca
      661 atgaaaaaaa cgttaaaatc aatgtccttg gccagaaaga aaaattcgcc ggcgatatca
      721 aagagcaaat agaaaaaata gaatcggaaa caaaaaacaa caccggcatg acattgaact
      781 tagctctcag ttacggcggg cgggcggaat tggtggacgc aatcaaaaaa atcgttaaaa
      841 agggaattga tccggataaa attaccgagg acaccgttaa agacaacctt tggacttcgg
      901 acgcggattt aatcatccgc accggcgggg aacagcggct atccggattt ttattgtggc
      961 aagcggccta ttcggaattt ctgtttgtga aaaaatattg gccggatttt ggcgaaaatg
     1021 atcttgacgc ggcgctggcg gaatacgcgt cgcgccagcg gcgcttcggc aaataatagc
     1081 attgttaacc gttaattttt aacaatggct ttgatttttg attttttaat tttgatttta
     1141 ttatgtctct taccatcatc aaaatcggcg gctcggtaat caccaaaaaa gaaagcaaaa
     1201 aaccaaaaat aaatgccgga aacatcaata agctcggcag tttattgaaa ggcttcaaag
     1261 agtcttatgt tttggtgcat ggcgtgggat ctttcggcca tccgttggtg aaacaaaccg
     1321 gcattgacaa aagcgtcatc agcgaaaaac agattctggc atttgctaaa actcaaaaat
     1381 tgcaaaacga tttgaattgc atggtttgca accgactgat aaaaaaaggc gtgcccgcgt
     1441 ttccggcgca agcttcaagc cacgcggtac tggaacgagg acgattggta tcaatggcca
     1501 cagaagctat gggagggctt ttgcgcctga aaatggtgcc ggtcgcctac ggcgtaccgg
     1561 cgtttgacaa agcttgggga tcggcaattc tctcgggaga tcaaatcacg ccctatattg
     1621 ccaaaaagat gggtgccgaa aagataattg aggtgagcga cgtggaagga attttcaccg
     1681 ccaaccccaa aatcaataaa aacgcgcggc tgattcccga aattaaccgc aaaaattatc
     1741 aggatattca aaaatgcctc tcgggaagca ccgccgccga tgtcaccggc ggaatgaaac
     1801 aaaaatacct tgaactgctg gacgcggcca aaaccggcat catctgccaa attgtagact
     1861 taaaacattt taaaaacgcc ctggaaggca aacccgccgg caccgtcatt aatttgcaac
     1921 aataatgaaa attgtcaaaa aagatcaaag aaaagaattt aaaaatagcg attgttgtct
     1981 tgcttatgag tatccaatgg atgattgcga tatcaacggc gcggtgatca aactttcggg
     2041 acgctatccc gacaaaggct gggcggccaa tgaagtttgc aaggaaatgg gctatatcat
     2101 ttcgggatcg gggaaattgg ttgtggagga taaagaatac gaattaaccg ccggcgattt
     2161 ggtcttgata aatcccggcg aaaaatttta ttggcagggc aatatggaaa tctttatgcc
     2221 ctgcgccccc gcctggtcgc ccgaccaata caaaaccgtc aaataattaa aaaaaacaaa
     2281 aaacaggggt acaacctctg ttttttaatt tttcccattg aattcttggt tggcacgcgc
     2341 cataatcaag aataggcgaa tcatttttca aatcaaaaac aattatgcaa attcaagacg
     2401 ccaaggagaa aatcagggag gcggtgcggg aattcaaggc gcttgaccgt gccgctcttt
     2461 tgaaagttaa tgtctatgaa ccggcaatca gccatcggat cgcggtatat ttgggaaatt
     2521 tgttccgcga actgaatgtt gattgcgaat ataaccgcaa cttattaacc gccaaaaaga
     2581 ataccaacgg ggacaaaatc cggcccgata ttatcattca cagaagatta agtccgaaca
     2641 attgcataat ttttgaaatc aaaaaaggcg gcaaagacag ccaaaaagcc attactgaca
     2701 tcaggaagtt agaggatgcc gtcgccggta atctcggtta tgatttggga gttttcatcg
     2761 gcattttgaa acgccgcatt gacatctgct ggattgaaaa aatcaacagc accttattca
     2821 aaacctgcga gaccatttag caataatcaa aaatttctgg cgggaaaagc aataaatttt
     2881 tgcaaccgaa agataaaaat tgtagctaag cggtaatttt ccgtaccgtt atttttaatt
     2941 atgaatctgt caggaattct aatattaata gcattggtat ttggcgctta tgcggcgttg
     3001 attctattgc gaaaaaaacg cttatcggat cttctgcaaa aattagctaa atatgatagg
     3061 gattttaaag tcgctattat agattttcac aaactttttc aaaataattg gtatataagc
     3121 gattggcaat atcaa
//
LOCUS       DAOQWE010000098         3082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_33757_length_3082_cov_3.920714, whole genome shotgun sequence.
ACCESSION   DAOQWE010000098 DAOQWE010000000
VERSION     DAOQWE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33757_length_3082_cov_3.920714"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKK37_03485"
     CDS             complement(<1..299)
                     /locus_tag="PKK37_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HNR81474.1"
                     /translation="MSQEIPTNKLSVYLIKEEYSEHKDILKKIDTLQSKNVNGVGVLY
                     FGESHTFKPSWLDKFFGSSLGDDIEKLFNASSKAILLAKVKVATNKERMFAIPFG"
     gene            complement(296..2524)
                     /locus_tag="PKK37_03490"
     CDS             complement(296..2524)
                     /locus_tag="PKK37_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81475.1"
                     /translation="MWIEFKNYQEKAIVKLKQEVNDLLDAEGNKICIFKSPTGSGKTL
                     MMAEFLKRLIDSRIDGKKFSFIWIAVNKLHDQSRNSLKKYYDPLGIGLKCSYFEDLDD
                     RKIGENEILFLNWASINKKGNIYVRENERDNNLSNIVARTKDEGRIIILVIDESHRNA
                     ETDKSKELIEDIGPKITIEVSATPQLKGVFRGVEVELKDVKDEGMIKKEIVINPGFEN
                     FKLDKKLADKTADEIVVEAGLKKRAELAKLYEVAGSNVNPLMLIQIPDSRKGLIDKKD
                     SVVQLLGKYGITTENGRLAIYLSDKDSKINLENIEKDENEVEVMIFKQAIAVGWDCPR
                     AAILVLFREWKSMIFSIQTIGRIMRMPEHYHYKNSELNLGYVYTSLSNIGIAEDIAKE
                     YITVFESKRRDDYKNIDLTSYHSKRFREETRLSSDFVPIFFKAADELKLKDKISLKHS
                     IVDTKLIASGKIYDVDKQTKSIEKKGTLDIPKNEVELQMAFDYFARENLAPFHPELRS
                     IKRINDSIYGFFGASRDEDRWPKIQAIVLAEENRQAVIDTINRAKELYQEVVGKGKHE
                     VIQNDEPWNVPEIINYNLTFVKKDYKRSIIQPYYAKTKGTDNLSLFEEDSDIEVSFIE
                     YLEKAKQVKWWFKNGKQDGSYFAVPYFENGQEKPFYIDFIVMLNDGRLGLFDTKGGIY
                     AKTAKERAEGLAEYIAAENKSDKKLFGGIVLKDKNSWRYNDSKKYSYDPNDLKDWKFL
                     DL"
     gene            complement(2530..>3082)
                     /locus_tag="PKK37_03495"
     CDS             complement(2530..>3082)
                     /locus_tag="PKK37_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81476.1"
                     /translation="FAGSGTTGHATLELNKEDGGNRQFILCTNNENNICTDVCYPRLK
                     KVIKGYKNGKDENVAGLGGNLKYYTCDFVEAEPTDRNKRKLVNESTEMLCIRENAFEL
                     VQDESDFKIFKNSDKYLGIVFYEEAIDDFKKAIKKIKGHFNTYVFSLGDDPHEKQFAD
                     VKSKVTLCAIPEVILKVYREIFK"
BASE COUNT          805 a          687 c          540 g         1050 t
ORIGIN      
        1 tagccaaagg gaattgcaaa cattctttct ttattcgttg caactttaac ttttgccaat
       61 aaaattgctt tagaggaagc attaaataat ttttctatat catcgcccaa agaggaaccg
      121 aaaaacttat ctaaccacga cggcttgaaa gtgtgagatt cgccaaaata aagaacgcca
      181 actccattaa cattttttga ttgaagcgta tcaatctttt tcaaaatatc tttatgctcg
      241 gagtattcct ctttgataag ataaaccgat aacttgtttg ttggtatttc ttggctcata
      301 aatctaaaaa tttccaatcc ttcaaatcgt ttggatcgta agaatatttt ttgctgtcgt
      361 tatatcgcca gctattttta tctttcaaaa ctattccgcc gaatagtttt ttgtcgcttt
      421 tgttttccgc cgcgatgtat tccgccaatc cttcggctcg ttctttcgct gttttggcgt
      481 aaattccgcc ctttgtatca aacaagccaa gccgtccgtc gttgagcata actataaaat
      541 caatataaaa cggcttttcc tgtccgtttt caaaataagg cacggcaaaa taagagccgt
      601 cttgtttgcc attcttaaac caccatttca cctgttttgc cttttccaga tactcaataa
      661 aggaaacttc aatgtcgctg tcttcttcaa atagcgagag attatccgtg ccttttgttt
      721 tggcgtaata cggctgaata attgaccgct tataatcttt ttttacaaaa gtcagattgt
      781 aattgattat ttccggcaca ttccacggct cgtcattttg gatgacttca tgtttgcctt
      841 tgccaactac ttcttgatat agctctttgg cccgattgat tgtgtcaatc acggcttgac
      901 gattttcttc ggccaaaacg atagcttgga ttttaggcca tctatcttca tctctgcttg
      961 caccaaaaaa tccgtaaatt gaatcgttaa tgcgttttat tgagcgaagt tcagggtgaa
     1021 aaggcgcaag attttcacgc gcgaaataat caaaagccat ttgtaattca acttcgtttt
     1081 taggaatatc cagcgtgcct tttttctcaa tactctttgt ctgcttgtcc acatcgtaaa
     1141 tcttcccgct cgcgataagt ttcgtgtcca caatgctatg tttgagcgaa attttgtctt
     1201 tcagtttcaa ttcgtccgcc gctttgaaaa aaatcggcac aaaatcggac gacaaacggg
     1261 tttcctctcg gaaacgcttg gagtgatagg aagtcaaatc aatattttta taatcatcgc
     1321 gccgcttact ttcaaaaacc gtaatgtatt ctttggcaat atcttcggca attccaatat
     1381 tgcttaaact ggtataaaca tagccgagat taagttctga atttttgtaa tgatagtgtt
     1441 cgggcatacg cataatgcgc ccgattgtct gaatggaaaa tatcatactt ttccactcgc
     1501 ggaacaaaac caaaattgcg gcgcgcggac aatcccagcc gaccgctatg gcttgtttga
     1561 aaatcatcac ttcaacttcg ttttcatctt tctctatatt ttcaagattg atcttactgt
     1621 ccttgtcgga aagataaatc gccagccgtc cgttttccgt agtgataccg tattttccga
     1681 gcaactgcac aactgaatct tttttatcaa tcagcccttt acggctgtcc ggaatttgaa
     1741 tcagcataag cggattaaca tttgatcctg ccacttcgta tagtttggca agttcggcgc
     1801 gtttcttcag tcctgcctca acgacaatct catcagccgt tttgtccgca agttttttgt
     1861 ccagcttaaa attttcaaaa cccggattga tgacgatttc tttctttatc atcccctcgt
     1921 cttttacatc tttaagttcc acttccacgc cgcgaaatac gcctttgagt tgcggcgttg
     1981 ccgaaacctc aatcgttatt tttgggccaa tgtcttcaat gagttctttt gatttgtccg
     2041 tttccgcgtt gcggtggctt tcatcaatga caaggataat aatccgccct tcgtcttttg
     2101 tgcgggcgac tatgtttgaa agattattat cccgctcgtt ctcacgaaca taaatattgc
     2161 ctttcttgtt aatgctcgcc cagtttaaaa acaaaatctc attttcgccg attttcctat
     2221 cgtccaaatc ttcaaagtaa gaacatttaa gaccgattcc gagagggtcg taatactttt
     2281 tcaagctgtt gcggctttgg tcgtgcagtt tattgacggc aatccaaata aaagaaaact
     2341 ttttgccgtc aatgcgtgaa tcaatgagcc gcttcaaaaa ttcggccatc atcagcgttt
     2401 tgccggaacc tgtcggcgac ttaaaaatgc agattttgtt tccttccgca tccaaaagat
     2461 cgttgacttc ctgcttcaac tttacgattg ctttttcttg ataattttta aattcaatcc
     2521 acatagtatt tatttaaaaa tctccctata tactttcaag attacttcgg gaatagcgca
     2581 aagcgtaacc ttgcttttga catcggcaaa ttgtttttcg tgcgggtcgt cgccaagcga
     2641 aaaaacataa gtgttaaaat gccctttgat ttttttaatc gctttcttga agtcgtctat
     2701 cgcttcctcg taaaaaacaa tgccgagata tttatcgctg tttttgaaaa tcttaaagtc
     2761 gctttcgtcc tgcaccaatt caaaggcatt ttcacggata caaagcattt ccgttgattc
     2821 attaacaagt tttcgcttgt ttctgtcggt cggctcggct tccacaaagt cgcaagtgta
     2881 atacttcaga tttccgccaa gtccggcgac attttcatct ttgccgttct tatacccttt
     2941 gataactttt ttcaaacgcg gatagcaaac atcggtgcaa atattatttt cattgttggt
     3001 acaaagtata aattgccgat ttccgccgtc ttctttgttt aattccaaag tggcgtggcc
     3061 agttgtacca gagccagcaa aa
//
LOCUS       DAOQWE010000099         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_34183_length_3055_cov_5.623333, whole genome shotgun sequence.
ACCESSION   DAOQWE010000099 DAOQWE010000000
VERSION     DAOQWE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34183_length_3055_cov_5.623333"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..701
                     /locus_tag="PKK37_03500"
     CDS             30..701
                     /locus_tag="PKK37_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNR81477.1"
                     /translation="MLTIDAKIRELKGRKTEELRREGMIPAVLYGPGIENKDLTVTGK
                     EFANLFRQAGKSSLVGLRVEDEAKNFMVLINDLSVDPVSGKTIHIDFYQPDLKKEIEA
                     DVPLVFVGESPAVKDLGGTLIKNFDEIAVKALPADLPREIKVDISVLKNFDDAIAVKD
                     LTVAGKVELLKNPEEIVALAAEAQKIDEELEKPIEEDVDSVAKVVKEKKEEVSEEEAA
                     PSGKK"
     gene            790..>1730
                     /locus_tag="PKK37_03505"
     CDS             790..>1730
                     /locus_tag="PKK37_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81478.1"
                     /translation="MKIFRFNIAALIMAAMFLCGSIAAAQTNGNGETGECTTKESCQA
                     LLGKYEALLKQYDSDITKTAAEKKTLQNQIAALKKKADSLAVQIKQSNAMIGEITLQI
                     ADTEASVDTTETKIQKTQKQLAAVLRAIDQADKVSLIENLIAGNNFSDFFDNLVYLEN
                     LDRKSNQVLQEIKGLKQSLEDQKLGLEGEKTDLEKTTQMKLLQKQEHEKAQAEYNSTL
                     KVKDAELAQTTQAKKLTAQVIQQIKQRLFSLAGVADTDAPNFEQAYQIAKSAGAAVGV
                     RPAFLLAILTQESSLGKNVGGCYMTNDATGAGVKISTG"
     assembly_gap    1731..1830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2042..2215
                     /locus_tag="PKK37_03510"
     CDS             2042..2215
                     /locus_tag="PKK37_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81479.1"
                     /translation="MDRKNSEKNIRKLTRAGKVSLSLTIPRDLVVELGWRDNQKVVVK
                     KRGQGLAVEDWKE"
     gene            2324..>3055
                     /locus_tag="PKK37_03515"
     CDS             2324..>3055
                     /locus_tag="PKK37_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81480.1"
                     /translation="MANSKEAKARIKINKLLEYSGWRFEDDKNGKANIRLEPGVKYTD
                     LGDDFEHEVHGFIDFLLLDKDGRALVVVEAKRESIDPLSAKEQARNYARNSGARFVIL
                     SNGNLHYFWDTKHGNPDAISRFPTQDSITQYEKYTPNPVELAQTFVDENYIVESQMPG
                     YAGDPLFRDEARRSEFLKNNNLKQLRPYQAQAIKSLQEAAMAGKQRFLFEMATGTGKT
                     LISAAAIKLFLKSGNARRVLFLVDRL"
BASE COUNT          860 a          648 c          797 g          650 t
ORIGIN      
        1 tctttctgcg aagaagataa taaatcaata tgttgacaat tgatgcgaaa atcagggaat
       61 tgaaggggcg caagaccgaa gaattgcgcc gtgaaggaat gattccggcg gtgctttacg
      121 gtccgggaat tgaaaacaag gatttgacgg tgacgggcaa ggagtttgcc aatctcttcc
      181 gacaggccgg caaaagttct ttggtggggt tgcgcgtgga agacgaagcc aaaaatttta
      241 tggtcttgat caacgatttg agcgtggatc ccgtgagcgg taaaacgatc cacattgatt
      301 tctatcagcc tgatctgaaa aaggaaattg aggccgatgt gccgctggtt tttgtcggcg
      361 aatcgccggc ggtaaaggat ttgggcggca ccctgatcaa gaattttgac gagatcgccg
      421 tgaaagcttt gccggccgat ttgccgcgcg aaatcaaggt ggatatctcc gttcttaaaa
      481 attttgatga tgcgatcgcc gtcaaagatt tgacggtggc cggaaaggtg gaactgctca
      541 aaaatccgga agaaatcgtg gcgctggcgg cggaagcgca aaagattgac gaggagctgg
      601 aaaagccgat tgaagaggat gtggacagcg ttgccaaagt ggtgaaggaa aagaaggagg
      661 aagtgagtga agaggaggcg gccccttcgg gaaagaaata atgccgatgt tttgcgcgcg
      721 cggttgccaa accgcgcgcg cttttgttat aatcaagtgt gtttgggtct gtctaacgca
      781 aataccatta tgaaaatttt ccgatttaat atcgccgcgc tgattatggc ggcaatgttt
      841 ctttgcggga gcattgcggc ggcgcaaacc aacggcaacg gcgaaaccgg cgagtgcacc
      901 accaaggaat cttgtcaggc gcttttgggc aaatacgaag cgcttttgaa acaatatgat
      961 tccgatatca ccaaaaccgc ggcggaaaag aaaaccctgc aaaaccagat cgcggcgttg
     1021 aaaaagaaag ccgattcgct ggcggtgcag atcaagcaga gtaacgcgat gatcggcgaa
     1081 ataacgcttc aaatcgccga caccgaggct tccgtggaca ccaccgaaac aaaaatccaa
     1141 aaaactcaaa aacaactggc ggcggtcttg cgggcgattg accaagccga caaggtttct
     1201 ttgattgaaa atttgattgc cggcaataat ttttcggatt tttttgacaa tttggtttat
     1261 ctggagaacc ttgaccgcaa atcaaaccag gttttgcagg agattaaggg cttaaagcaa
     1321 tctctggaag atcagaaatt gggtttggaa ggcgaaaaaa ccgatttgga aaagaccacg
     1381 cagatgaaac tcctgcaaaa acaagagcac gaaaaagcgc aagccgaata taacagcact
     1441 ctgaaagtga aagacgccga attggcgcaa accacacagg ccaaaaagct cacggcgcag
     1501 gtgatccagc agatcaagca aagattgttt tctttggccg gcgtcgccga caccgacgcg
     1561 ccgaattttg agcaggccta tcagatcgcc aaaagcgcgg gagccgcggt cggcgtgcgg
     1621 ccggcatttt tgttggcgat tttaacgcag gaatcgtcgc tgggaaagaa tgtgggcgga
     1681 tgctatatga ccaacgatgc caccggcgcg ggagtgaaaa tcagcaccgg nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgtatgtttc cgactatggc gccacttcgc
     1861 agacctatga cggcgaatgg cgcgcggcaa tgatctattt tgccggcacg gtgaacacca
     1921 aataccgctt ctacggcgat aatgtgatga agatcaccca aggctacgcc gccgacattg
     1981 cggccattga aggccgctaa gtcgtattta aatacgattc aaacacgatt tcaaaaacaa
     2041 tatggaccgc aaaaattccg agaaaaacat ccgcaaactc actcgcgccg gcaaagtgtc
     2101 gttgtcgctt acgataccgc gcgatttggt ggtggagctt ggctggcgcg ataaccagaa
     2161 agttgtcgtc aaaaaacgcg gccaaggcct tgcggttgaa gattggaagg aatgaaacca
     2221 attccttaac ttgtttgacc gttgcaaaaa cgcggcggaa cgccgcgttt ttaacataaa
     2281 taaatcattg atttttaagc caaaaggggg taatttgaag aatatggcaa attcaaaaga
     2341 agcgaaagca cgaataaaaa taaacaaact gcttgaatat tccgggtggc gttttgaaga
     2401 cgacaaaaac ggcaaagcga atatccggct tgaacccgga gttaaatata ctgaccttgg
     2461 cgatgatttt gaacatgaag ttcacggatt tattgatttt cttttgctgg acaaggatgg
     2521 ccgggcgctg gttgttgtgg aggcgaagcg cgaatcaatt gatccgcttt ccgccaaaga
     2581 gcaagcgcga aattacgcgc gcaacagcgg cgcgcggttt gtgatccttt ccaatggcaa
     2641 tctgcattat ttttgggaca ccaagcacgg caatcccgac gcgatatccc gctttccgac
     2701 gcaggattct attacccaat acgaaaaata cacacccaat cccgttgaac tggcgcaaac
     2761 ttttgttgac gaaaattata tcgtggaatc gcaaatgccc ggatatgccg gtgatccttt
     2821 gtttcgagat gaagcaagac gatccgaatt tttgaaaaat aacaatctta aacagctccg
     2881 cccgtatcaa gcacaggcga taaaatcgct gcaggaagcg gcaatggctg gcaagcagag
     2941 atttttgttt gaaatggcca caggcaccgg caaaacgcta atttcggcgg cggcgatcaa
     3001 gctttttttg aaatcgggca acgcgcggcg cgtgctgttc ttggttgacc gcttg
//
LOCUS       DAOQWE010000100         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_34192_length_3055_cov_2.539333, whole genome shotgun sequence.
ACCESSION   DAOQWE010000100 DAOQWE010000000
VERSION     DAOQWE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34192_length_3055_cov_2.539333"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKK37_03520"
     CDS             complement(<1..865)
                     /locus_tag="PKK37_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNR81481.1"
                     /translation="MSKIRTTTWPIEPHTEAKHAILKKYLNAWVPIMARYNGRVLYID
                     GFAGPGEYEGGEPGSPIIAINAVLGQRISIKSEIIMFFIEENEDRCAHLNQKISTFNI
                     PQNINLKPKVVCGKFDETLSNIFKFLEEQKKSIAPSFIFIDPFGFNMPFSVIKKIMSN
                     GKCEVLINFMYEKINRFIGDKKLEKHYIELFGTDKWKTATTENDPKKRIEILHSVYLE
                     QLKNEAGIKFVHSFKMINKVNKTYYFLFFGTNNLLGIEKMKEAMWSVDKTGNFQFSDA
                     THNCSQMTLFDN"
     gene            complement(889..1413)
                     /locus_tag="PKK37_03525"
     CDS             complement(889..1413)
                     /locus_tag="PKK37_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81482.1"
                     /translation="MVLLEAIYSLILAVIPVISDNWISFCNNLTKWVLAIAGYQYSGW
                     IFSGILITIFVVFLWRMKKKNKGCAITFISAPPSQEHFRYGKPELFNVSWDLWSGSDS
                     LFDSGPEHERVWVDGPYCIENDCGYELDREGDKWVCAKCGKNYKIPKHIQNDTRDKVI
                     KIFESEIKKIPGKA"
     gene            complement(1489..>3055)
                     /locus_tag="PKK37_03530"
     CDS             complement(1489..>3055)
                     /locus_tag="PKK37_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNR81483.1"
                     /translation="IASGEFIEEQIYNWTYSDEQKAKGSWEEKNGPNPYAPLPRMVMM
                     TYQLPDSIIDIARQGEFNEFDLNEFFSADGEGAEAKFKYEDEVQKWLDLIRGSFLETT
                     IDNLKLGAKKPPLPYSHAELLNLLNHTLWFLPSVASCAAMRNLLAKKQNIFYKDYKVV
                     VAAGMGAGIGIKALPPVLEAMDDPLESKTITLTCGKLLTGVTVKPWTGIFMLRSCSTP
                     ETYFQAAFRVQSPWTVKNPDNISPNKEEIIKRECYVFDFAPNRALRQIAEYSCRLNVN
                     ETNPEAKVAEFISFLPVLAYDGSAMKQIDAAGVLDMAMSGTTATLLARRWESALLVNV
                     DNGTLKRLMDNDAAMDALMSIEGFRSLNQDIETIINKSESVEKIKKEANDKELSEKKK
                     KELTEEEKEYKSVRKQIQVKLIKFATRIPVFMYLTDYRERALEDVITKLEPGLFKKVT
                     GLTQKDFELLVSLGVFNKALMNDAVYKFKRYEDASLAYAGVNKHEGENIGLFDTVITP
                     KDYGQTFENEPAG"
BASE COUNT          819 a          692 c          552 g          992 t
ORIGIN      
        1 tattatcgaa taaagtcatt tgcgaacaat tatgtgttgc atcagaaaac tgaaaattac
       61 cagttttatc aacgctccac atcgcttctt tcattttctc tattcccaac aaattatttg
      121 taccaaaaaa taaaaaataa taagttttat ttaccttatt gatcatttta aatgaatgaa
      181 caaattttat gccagcttca ttttttaatt gttccaaata gacagaatgg agtatttcaa
      241 tccttttttt agggtcattc tctgttgtcg cagttttcca tttgtcagtg ccaaacaatt
      301 cgatataatg cttttcgagt tttttatcgc cgataaaacg atttatcttc tcatacataa
      361 aattgattaa tacttcgcat ttgccattac tcattatttt ctttatcacc gaaaacggca
      421 tattgaaccc aaatggatct atgaaaataa aagagggggc aatacttttt ttctgttctt
      481 ctaaaaattt aaaaatatta ctcaatgttt catcaaattt gccgcaaaca acttttggtt
      541 taaggttaat attttgagga atattaaaag tactaatttt ttgattaagg tgagcgcacc
      601 tatcttcatt ttcttcaata aaaaacataa ttatttccga ttttatcgaa attctttgtc
      661 ctaacaccgc gttaatggct ataattggag aacctggctc tcctccttca tattcaccgg
      721 ggccagcaaa gccatcgata taaagcacgc gcccgttata tcttgccatt atgggtaccc
      781 aagcatttaa atattttttc aaaattgcat gttttgcttc ggtatgcggc tcaattggcc
      841 aagttgttgt tctgatttta gacatacatt taattctgat gcgattcttt atgcttttcc
      901 tggtattttc ttaatttctg attcaaatat cttgattacc ttatctctcg tatcattttg
      961 aatatgtttt ggaattttat aattcttgcc acatttagcg catacccatt tatctccttc
     1021 tctatctaat tcatatccgc aatcattttc tatacaatat ggaccatcta cccataccct
     1081 ttcatgctct gggccggaat caaaaagaga atctgacccg ctccatagat cccaggaaac
     1141 attaaacagc tctggcttcc cataacgaaa atgctcttga cttggcggtg ctgaaataaa
     1201 tgtaatggcg catcctttat tttttttctt cattctccac aaaaaaacca caaaaatagt
     1261 aatcaagatg ccggaaaaaa tccatccact atattgatag ccagcgatag ctaaaaccca
     1321 tttcgttaaa ttattacaaa atgaaatcca attgtcgctt ataactggta taacggccaa
     1381 tattaaactg tatattgctt caagtaacac catcctgtta atttacatta gttcggtttt
     1441 cattgataaa attccgaatg tgaagatacc ggtgtataga tatatttatt atccggcagg
     1501 ctcattttca aatgtttggc cgtagtcttt gggggtaatg acggtgtcaa aaaggccgat
     1561 gttttcgcct tcgtgcttgt tgacgcctgc gtaggccaag ctggcgtcct cgtagcgttt
     1621 gaatttatag accgcgtcgt tcatcaaggc cttgttgaaa acgcccaagc tcacaagcaa
     1681 ttcaaaatct ttttgagtca gcccggtaac ttttttgaac aaacccggct ccagcttggt
     1741 gattacatcc tccaacgccc tttcgcggta atccgtgaga tacataaaaa cgggaatccg
     1801 ggtcgcgaac tttatcaatt tcacttgtat ttgttttctg acgcttttat attccttttc
     1861 ttcttcggtg agctcttttt tctttttctc ggatagctct ttgtcgtttg cttccttttt
     1921 tattttctcc acggattcgg atttgttgat aatggtttca atgtcttggt ttaaacttcg
     1981 gaaaccttca atgctcataa gagcgtccat cgccgcgtca ttatccatca atcgcttgag
     2041 cgtgccgtta tccacattca caagcaacgc gctttcccat cgtcgcgcga gcaaggtagc
     2101 ggttgtgccg ctcattgcca tatccaaaac cccggcggcg tctatttgtt tcatcgcgct
     2161 gccatcgtag gcaagcaccg gcagaaagct aataaattcc gcgactttgg cttccggatt
     2221 ggtttcgttc acattgaggc gacagctgta ttccgcgatt tgccgcaacg cccgattggg
     2281 cgcgaaatca aagacataac attccctctt gataatctct tccttgttgg gcgagatatt
     2341 gtcgggattc ttaaccgtcc acggcgattg gacgcggaaa gcggcttgaa aataggtttc
     2401 gggcgtggaa caactgcgaa gcataaaaat tcccgtccac ggcttgaccg tcacgccggt
     2461 taaaagcttg ccgcaagtta aagtgatggt tttggactct aatggatcgt ccattgcttc
     2521 cagaaccggc ggcaaggcct ttatgccgat accggcgccc atccccgccg cgacgaccac
     2581 tttgtaatct ttataaaaaa tgttttgctt tttcgccagc aaattgcgca tcgccgcgca
     2641 cgaagccacg gacggcaaaa accaaagcgt atgattgagc aaattcaaca gttcggcgtg
     2701 ggaataaggc aaaggcggct tcttggcgcc cagcttcaaa ttatcaattg tcgtttccaa
     2761 aaaagacccg cggatcaaat caagccattt ttgcacctcg tcttcatatt tgaatttcgc
     2821 ctccgcgccc tcgccatcgg cggaaaaaaa ttcattcaaa tcaaattcgt taaactcgcc
     2881 ttgccgggca atgtcaatga ttgaatcggg aagctgataa gtcatcatca ccatgcgcgg
     2941 aagcggcgcg tatggattcg ggccgttctt ctcttcccat gaaccttttg ctttttgctc
     3001 gtcagaatat gtccagttgt atatctgctc ttcaataaat tccccggagg caatc
//
LOCUS       DAOQWE010000101         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_34239_length_3052_cov_8.047047, whole genome shotgun sequence.
ACCESSION   DAOQWE010000101 DAOQWE010000000
VERSION     DAOQWE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34239_length_3052_cov_8.047047"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PKK37_03535"
     CDS             <1..343
                     /locus_tag="PKK37_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81484.1"
                     /translation="KIWLAVFLAMFLVPEILWSPIGNFYYLIFFAEEWNEQTAQFFNM
                     ASLPDSGIIRLIIGIVEIIGLLLFVGTILFFYRGRHKFIKIFAIILMLILLLLLFYVF
                     IFSLNFNPRVG"
     gene            469..612
                     /locus_tag="PKK37_03540"
     CDS             469..612
                     /locus_tag="PKK37_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81485.1"
                     /translation="MEINFTEGYYGVLTLPVGRYWLWSSSGDVVVYSCDEGGVSDPKP
                     VWR"
     gene            802..1329
                     /locus_tag="PKK37_03545"
     CDS             802..1329
                     /locus_tag="PKK37_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81486.1"
                     /translation="MVKKTKTILIILVAFVIAPVLLGIIYLAFIAINFYQINHNPKYK
                     FNSLPCSSVSRVELGSPQLYDYKKSPAAEFSTNGADIYISAQTSDDRDSPLDFFPSAT
                     IYIGDISILPEHGSQNEINIDNVIKINYYGGYYKYSENISLPAGRYRLWIASGREVIL
                     YSCEEGGVSDPKPVR"
     gene            1413..>3052
                     /locus_tag="PKK37_03550"
     CDS             1413..>3052
                     /locus_tag="PKK37_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81487.1"
                     /translation="MIGGRKAKKVLWCGLAAVAMIALLPFFARADNYGDQAAFNIDKQ
                     YDSAGRSQLSASLVWAGEKIYFYADNAWWNDLDPLERQRYGAVLSGLDAEFSRHIQPK
                     LTELFGASAGSGPSRNGKLTVLIHQMAKGTGGYINTADGYSKYQSPGSNEREMVYFNT
                     SFIDAPLAKTYLAHEYTHLITFNQKERLRHVIEEVWLNEVRAEYSSTILGYDASFGGS
                     NFQQRVKSFLNDPGKSLTEWENVPANYGAAHLWAQYVVDHYGVAILADSMKSDQTGIA
                     SIDYALKKNGFDVDFAQVFRDWIITLAANDCSIGPKYCYKYPDLREFVVSPRINYLPA
                     SDQVSLSVTYSTPYFAGNWQKITGGSGNLRLDFAAAAEHKFAVPYLTCDAAGKCSAGE
                     LETAADGNAMLKIEDFGRRYSSLVLMPFGSGKNFGFGSGTGTMLPYSFKISIVPTEVA
                     PSGGAENEKLQIEALQKQVDALKAEIARVSAILAARVSAAPKFSCGVINADLYSGAAN
                     RAQIVCLQEFLRSQGSLIYPGGLLSGNFDAATRAAVIR"
BASE COUNT          782 a          625 c          759 g          886 t
ORIGIN      
        1 aaaaatatgg ctggcggttt ttttggcgat gtttctggtg ccggagattt tgtggagccc
       61 gataggtaat ttttactatt taatcttttt tgctgaagaa tggaatgaac aaactgccca
      121 attttttaat atggcgtcac ttcccgactc cggcattatt cgtttaatca ttggtattgt
      181 tgaaattatt gggttattat tgttcgttgg taccattttg tttttttatc gcggccggca
      241 taagtttatt aaaatttttg ccattatttt aatgttaatc ttgcttttgt tattgtttta
      301 tgtttttatt ttctcattaa attttaaccc tcgtgttggc taaaatataa aattatattt
      361 gccgatgttt tccgctgatt attatcgtgg caagagtcaa aaagatgaaa tgaacaaaaa
      421 attaaagaga attttgataa tcaaagaaat ataatcagca atgtttctat ggaaattaat
      481 ttcaccgaag ggtattacgg cgtgctaact ttgccggtgg ggaggtattg gctttggtca
      541 agctccggag acgttgttgt ttacagctgc gatgagggcg gggtgagcga tcccaaaccg
      601 gtgtggcggt gaagcggact ggatcaaatt tttcagaggg ttagaccctc tgaatttccc
      661 aaaaaccagg atcgcggaat acaaaaaccg aaaactcaat ctgaaaaccc attccgggtt
      721 aaaactgctc aagtttaata ccaacaatgg caaactgaac tggagctatt aagccgtgtt
      781 aaaaatattt atatctatcc tatggtcaaa aaaacaaaaa cgatcttgat tatccttgtc
      841 gcattcgtta ttgcacctgt attattggga attatttatt tagcatttat tgccattaac
      901 ttttatcaaa taaatcataa tcccaaatat aaattcaatt ctttgccatg tagctctgtt
      961 agccgagttg agttgggttc tccacagtta tatgattaca agaaaagccc agcggccgaa
     1021 ttttcaacta acggcgccga tatctatata tcggcacaaa cttctgacga tagagattct
     1081 cctcttgatt ttttcccttc agcaactatt tatatcggag atatttctat tcttccagag
     1141 cacggatcgc aaaatgaaat taatattgat aatgttataa aaataaatta ttacggagga
     1201 tattataaat atagcgaaaa tatatctttg ccagccgggc gttatcgttt atggatagca
     1261 tcaggaagag aggtcatcct ctacagctgt gaggagggcg gggtgagcga tcccaagccg
     1321 gtgcggtgat gtaatcttgc gtttgtcggc tgaatttggc tttttggcgc ttttgaagta
     1381 tatttaaaat gtgtttaatg aggcgtaatg aaatgattgg agggcggaaa gccaaaaaag
     1441 ttttatggtg cgggttggcg gcggtagcga tgattgcgct gttgccgttt tttgcgcggg
     1501 cggataacta tggcgatcag gcggcgttca acattgacaa gcaatacgat tcggcgggaa
     1561 gaagccagct ttcggcgtcg ctggtctggg cgggggagaa gatatatttt tacgccgaca
     1621 atgcctggtg gaatgatttg gatccattgg agcgccagcg gtatggcgcc gttctttcgg
     1681 gtttggacgc ggaattttca cggcacatcc aacccaagct cacggaactt ttcggcgcca
     1741 gcgccggttc cggcccctcc cgcaacggca aactcacggt tctgattcac caaatggcca
     1801 aaggcaccgg cggctacatt aacacggcgg atggctattc caaataccaa tctcccggat
     1861 caaacgaaag agagatggtt tatttcaaca cctcttttat tgatgcgccg ctggcaaaaa
     1921 cttatctggc gcatgaatat acgcatttga tcactttcaa tcagaaggag cgattgcgtc
     1981 acgtgattga agaagtctgg ctcaacgaag tccgcgccga atattcttcc acgattttgg
     2041 gttatgacgc gtcgttcggg gggagcaatt tccagcaacg ggtgaaatcc tttttaaacg
     2101 accccggtaa aagcctgacg gaatgggaaa acgtgccggc aaactacggc gcggcgcatt
     2161 tgtgggcgca atatgtcgtt gaccattatg gcgtcgcgat attggccgat tccatgaaaa
     2221 gcgatcagac gggaatcgct tccattgatt acgctctgaa aaaaaacggc tttgacgttg
     2281 attttgccca agtctttcgc gattggatca tcacgctggc ggccaatgat tgttcgatcg
     2341 gcccgaaata ttgttataaa tatccggatt tgcgggagtt tgttgtttct ccccggataa
     2401 attatctgcc ggcttcagat caagtgtctt tgtcggtgac ctacagtacg ccctattttg
     2461 ccggtaattg gcaaaagatc acgggtggaa gcggcaattt gcgccttgat tttgccgctg
     2521 cggccgagca caaatttgcg gtgccttatc tgacctgcga tgccgccggg aagtgctccg
     2581 ccggcgaatt ggaaaccgcc gcggacggca atgcgatgct gaaaattgaa gatttcggcc
     2641 ggcgttattc ttcgctggtt ctgatgccgt tcggctcggg aaaaaatttc ggctttggtt
     2701 cggggacggg aacaatgttg ccttattcgt ttaaaatttc aatcgtgccg actgaagtgg
     2761 caccatccgg aggggcggaa aacgaaaaat tgcaaattga ggcgttgcaa aaacaggttg
     2821 atgcgttaaa agccgagatt gcccgcgtga gcgcgatttt ggcggcgcga gtttcggccg
     2881 cgccgaaatt ttcctgcggc gtgattaacg ccgacttgta ttccggcgcg gcaaaccgcg
     2941 cgcagatcgt ttgcctgcaa gaatttttga gatcgcaagg cagtttgatt tatccgggcg
     3001 gacttttgag cggcaatttt gacgccgcca cccgcgccgc ggtgatccgc tt
//
LOCUS       DAOQWE010000102         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_34603_length_3029_cov_5.261937, whole genome shotgun sequence.
ACCESSION   DAOQWE010000102 DAOQWE010000000
VERSION     DAOQWE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34603_length_3029_cov_5.261937"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1794
                     /locus_tag="PKK37_03555"
     CDS             <1..>1794
                     /locus_tag="PKK37_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81488.1"
                     /translation="NNTSQYCAAPDNPPTADGWLRTPGENCPKDGSGCNRDTGLCEGG
                     KTADCSNTPKGCLMAADDCEDCGAGWTCKLRSEVLNATDYKAWGLNATKTQISAVSGG
                     DGICVKGAGSGTEEEPEKTGKCDFKINALKNACSTSNSKCRSGVYVPFTITETSGNCD
                     DLSCTVTNDFDNTSVDATKASAGWWACSYPQSKSSGGDSEGTKGSDPSNLKFTIDCDG
                     KKATMTCPFPTADSNKFCEEEEEAQEEEKAEEGEKCKKDEDCGEGLVCLDGECVTEED
                     AEEKEEQEKQDKTAPKVTISSPSGTINTKTAELSVSTDIEAECKYMLSRDGSFNDFSG
                     GMFMSGGGGKSHTATLSNLTGVTAADCKYNHTVKVMCKNSKASQDAKAAVGSAQNNFS
                     VDLSQDSANAPAVSSAPMETKYTVANPVLKVTTDRPADCEYKKDGDFTFGGGTKFDTT
                     GNYTHNTQLNGLAAGDHTYYVVCKDKETCAVSKPGLPVKFAVDLSQDPANAPNIANTT
                     PETQTVANPTLSVSTDRPATCQYKKDATFIYGGGTQFANDGEYAHSVVLTDLPDGKYT
                     FYVACKDKATGAVKTLENPIITTLDRGQGKP"
     assembly_gap    1795..1894
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1895..>3029
                     /locus_tag="PKK37_03560"
     CDS             <1895..>3029
                     /locus_tag="PKK37_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81489.1"
                     /translation="GAIPTISNNTPNNQTVISPIISIITVAAANCQYNKDTDFTFGQG
                     TPFSTTGGTNHSATLPNMANGTYTYYVICKDAASGQTNTSGTQVIFTVNTAANAPVIS
                     NTTPASQSTSNPTLSVLTDRAAVCQYNKDADFAYGAGTQFTIDGGTGHSAVLSNIADG
                     THTYYVVCADSATQASNSPGAQIMFSVSTAAEVCADISSNDKQSDIDRDYGDDADYDS
                     AYIWRSSPAGVLEMFEKVDWYAGYQFTPEKNGSIAELCGYFDSGSRNRVSLYDGAYKE
                     IAGVEIDGQGGWSCAAISPAAVKADRRYYVIARVQDDAIYFAYDSGMLPRDTEGAVVD
                     SGVRQLASAGDFGESLKKYDYMVFGLVDAKIRFVDENSQGPEIS"
BASE COUNT          762 a          752 c          848 g          567 t
ORIGIN      
        1 aaacaatacg tcgcaatatt gcgcggcgcc ggataatcct ccgacagccg acggatggtt
       61 aagaacgccg ggggaaaatt gccccaaaga cggcagtggt tgcaatagag acaccggtct
      121 ttgcgagggg ggcaaaacgg cggattgttc aaacacgccg aaaggttgtt tgatggcggc
      181 cgatgattgc gaagattgcg gcgcgggatg gacgtgcaaa ctgcgcagtg aagttctcaa
      241 tgcgactgac tataaagctt gggggcttaa tgccaccaag acgcaaatca gcgccgtaag
      301 cggtggcgat gggatttgcg tgaaaggcgc cgggagcggg acggaggaag agccggaaaa
      361 aacgggcaag tgcgatttta aaattaatgc gctgaaaaac gcctgcagta ccagtaacag
      421 caagtgccgc agtggagtct atgtgccgtt caccatcacc gaaacaagcg gcaattgcga
      481 tgatttgagt tgcacggtga ccaacgattt tgacaacact tcagtggatg ccaccaaagc
      541 ctcagcggga tggtgggcgt gttcctatcc gcagtcaaaa agttccggcg gcgattccga
      601 agggacgaag gggagcgatc cgagtaattt gaagttcacg attgattgcg acggcaaaaa
      661 agcaacgatg acctgtcctt tcccgaccgc ggacagtaat aaattctgcg aagaggagga
      721 agaggcgcag gaagaagaga aggccgaaga gggagaaaaa tgcaaaaagg acgaggattg
      781 cggcgagggg ttggtttgtc tggacggcga gtgcgtgacc gaggaggatg ccgaggaaaa
      841 agaagagcag gaaaagcagg acaaaaccgc accgaaggtg acgatttcct ccccttccgg
      901 cacaatcaac accaaaaccg cggagctgtc ggtatcaacg gacattgagg cggagtgcaa
      961 atatatgctg tcgcgcgacg gttctttcaa tgatttttcc ggcggaatgt ttatgagcgg
     1021 cggcggcgga aagagccaca ccgccacctt gagcaatctc accggcgtga cggcggcgga
     1081 ttgtaaatat aaccacactg tgaaggtgat gtgcaaaaat tccaaggcgt cccaagacgc
     1141 gaaggcggcc gttggttcgg cgcagaacaa tttttcggtg gatctttcgc aagattcggc
     1201 caacgcgccg gcggtatcct ccgcgccgat ggagaccaaa tataccgttg ccaatccggt
     1261 gttgaaagtg accaccgacc gaccggcgga ttgcgaatac aaaaaagacg gcgatttcac
     1321 tttcggcggc ggcaccaaat ttgacaccac gggaaattac actcacaaca ctcaattgaa
     1381 cggtcttgct gccggcgatc atacctacta tgtggtttgc aaagataaag aaacttgcgc
     1441 ggtgagcaaa cccggcttgc cggtgaaatt cgcggtggat ctttcgcagg acccggccaa
     1501 cgcgcccaat atcgccaaca ccactcccga aacgcagacg gtagccaatc ccaccctttc
     1561 ggtgtccacc gaccgcccgg caacttgcca atacaaaaaa gatgccacgt tcatttacgg
     1621 cggcggcacg caatttgcca atgacggcga atacgcgcat tcggtggtgt taacggatct
     1681 gcccgatggt aaatatactt tttacgttgc ctgtaaggac aaagccaccg gcgcggtcaa
     1741 gactttggaa aatccgatca tcaccaccct tgatcgcgga cagggaaaac ccaannnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccggcg cgatcccgac gatttccaat
     1921 aacacgccca acaaccaaac cgttatcagt ccgattatat ccataatcac tgtggcggcg
     1981 gcaaattgcc aatataacaa agacaccgat ttcacctttg gccaagggac gccgttttcc
     2041 acaacgggtg gcacgaatca cagcgccacg cttcccaata tggccaacgg cacttacact
     2101 tattatgtga tctgcaagga tgccgcttcc gggcagacga acacttccgg cacacaggtg
     2161 atattcacgg ttaacaccgc cgcgaacgcg ccggtgattt ccaacaccac gcccgccagc
     2221 caaagcacca gcaatccgac actctcggtg cttaccgatc gggcggcagt ttgccaatac
     2281 aacaaggacg cggattttgc ctatggcgcg ggaacgcaat ttacgattga cggcggcacg
     2341 ggccacagcg cggtgctttc caatatcgcc gacggcacgc acacctacta tgtggtttgc
     2401 gcggattcgg ccactcaggc gtcaaacagt ccgggagcgc aaattatgtt ttcggtgagt
     2461 accgccgcgg aagtttgcgc cgacatttct tccaacgaca aacaaagcga cattgaccgt
     2521 gattacggcg atgacgcgga ttacgactcg gcctatatct ggcggtcttc gcctgccggc
     2581 gtgttggaga tgtttgagaa agtggactgg tatgccggct atcaattcac gcccgaaaag
     2641 aacggcagta tcgccgaatt gtgcggctat tttgactcgg gatcgcgcaa tcgcgtgagt
     2701 ctgtatgacg gcgcttacaa agaaatcgcg ggcgtggaaa ttgacgggca gggaggctgg
     2761 agctgtgcgg ccatcagtcc ggcggcggtg aaagctgaca ggcgctacta tgtgattgcc
     2821 cgggtgcagg acgatgccat atattttgcc tatgactccg ggatgttgcc gcgcgacacg
     2881 gaaggcgcgg tggttgattc cggcgttcgc caattggcgt cggcggggga tttcggcgag
     2941 agcttgaaaa aatacgacta tatggtcttc gggctggtgg atgccaaaat cagatttgtt
     3001 gacgagaatt cgcagggtcc ggaaatatc
//
LOCUS       DAOQWE010000103         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_34656_length_3026_cov_8.492763, whole genome shotgun sequence.
ACCESSION   DAOQWE010000103 DAOQWE010000000
VERSION     DAOQWE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34656_length_3026_cov_8.492763"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /locus_tag="PKK37_03565"
     CDS             <1..2229
                     /locus_tag="PKK37_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81490.1"
                     /translation="RALQNYAKINKNKFHPELNDEDVVLIDLTCSGAKIETGPRPPVV
                     GDVASNQIAPNSQLQQALNKLGEAGLTPADVDLVTVGMGGNDAGFAEIIQACLLPNIL
                     RRVLEEYPNPPGEIKILVNMFASCKNIDSTFFNSDEAINALYGKEVWSQNKILETFTN
                     ARVIQANYPDILPRESKSDRYCGGLREKDINYARKKVEAINDRINQAQIAVAKNNSRL
                     ELADLQNSLGDNALCPGNSNDILANGTDEANLDAEIRRLLNLDGNGDAQSRALIDDLA
                     AAYNSWKQCLGVHFNVFDNGADCDTGEAMEQLNYYWQNVEQYLKDRLNNKLLPNLIKP
                     EQASSESNEIRYDRSKGLFHPNANGHEIIACNILAAHNKAGAQNCLLPAGQAVMDAVN
                     GASINNVPINISIAQTISLYLGGFTPGSIVPITILSSSKSLGSAVADENGIIDTQIVL
                     PEMSPGVHEIVLESQNGAGVGIGKRFWVEYPGKPRAGESYGIYLKNIATGEGEEINIV
                     YGDVDFANLTSDDDGGVFVELPVFEGQESFEVTAKNQTTGQSSQTKTEVEKTRQPLKS
                     FAVKTAFVKWQKAKNKNQEFTIAGQFELPQDQSKTALEEKLKLKLTFPDGKTATADLN
                     FKENNIFWKYSSLAENQNLKISRAVVYWSPEKKFCSNSKICAVALKNKNWFYLQGELE
                     NLGLEIGKTDKMEIEILLTAKTVDLVGGQKLPMGKVGNQRIYHSFGFWNFWPQNLESF
                     YK"
     gene            2235..2750
                     /locus_tag="PKK37_03570"
     CDS             2235..2750
                     /locus_tag="PKK37_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81491.1"
                     /translation="MNKKIKRVLTIIGLILIIVIIGIAIAMFMMFKELKGVVTSWSPS
                     GLDALPCEPKSQVELGSPYRINGQVAEFTTNGGKLYITAKRFPHSGFSGSSLGSTAIF
                     MGNSSALPEYDSQRDITTNTVKNISLSETKYVEFELPARRYWLWSSSGGDIVIYSCEE
                     GGVSDPKPVWR"
BASE COUNT          887 a          610 c          786 g          743 t
ORIGIN      
        1 cgcgctctgc aaaactatgc caagatcaac aaaaacaaat tccatcccga attgaatgat
       61 gaagatgttg ttttgattga ccttacctgc agtggcgcca agattgaaac cggcccacgc
      121 ccgccggtgg tgggcgatgt ggcaagcaac caaatcgcgc ccaactcaca gttgcaacag
      181 gcgctgaaca agctgggcga agccggcctg actcccgccg atgttgatct ggtcactgtc
      241 ggtatgggcg gcaacgacgc cgggtttgcg gaaattattc aagcctgtct tttgcccaac
      301 attttgcgcc gggttttgga agaatatcct aatcctcccg gagaaataaa aatattggtc
      361 aatatgttcg cttcttgcaa aaatattgac agcacctttt ttaacagcga tgaagcgatt
      421 aatgcgctct atggtaagga agtttggtcg caaaacaaaa ttttggaaac ttttaccaac
      481 gcccgcgtaa tccaagcaaa ttatcctgac attttgcctc gggaaagcaa gtcggatcgt
      541 tattgcggcg gcttgcggga aaaggatatt aactacgcga ggaaaaaagt ggaagcgatt
      601 aatgatagaa tcaaccaagc ccaaattgcg gtggcaaaaa acaattcgcg gctggaactg
      661 gcggatttgc aaaattcttt gggcgacaat gctctttgcc cgggaaatag taacgacata
      721 ctggccaatg gcactgatga agccaacctt gacgccgaga tccgccgttt gctcaatttg
      781 gatggcaatg gtgatgccca atctcgtgca ctgattgatg atttggcggc ggcgtataat
      841 tcctggaagc aatgtttggg tgttcatttt aatgtgtttg ataatggcgc ggattgtgat
      901 acgggagaag cgatggagca gttaaattat tattggcaaa atgtagagca gtatttaaaa
      961 gatagactta ataataaatt attgcctaat cttattaagc cagagcaagc tagcagtgag
     1021 agcaatgaaa tacgctacga tcgctccaaa ggtctgttcc atcccaatgc caatggccat
     1081 gaaattatcg cctgcaacat tctggccgcg cacaacaaag ccggcgcgca aaattgcttg
     1141 ctacccgcgg ggcaagcagt gatggatgcg gtcaatgggg cttctatcaa taatgtgccg
     1201 attaatattt ccatcgcgca aacaataagt ctttatctgg gtggctttac tccgggatca
     1261 atcgtgccga taacgatttt atcgtcgtcc aaaagtttgg gatcggcggt tgccgatgaa
     1321 aacggcatta ttgatacgca gattgttttg cctgaaatga gtccgggcgt gcacgaaatt
     1381 gttctggaga gccaaaatgg cgccggcgtt ggtatcggca agcggttttg ggttgaatat
     1441 cccggaaagc cgcgcgccgg agagagctac ggcatctatt taaaaaatat tgccaccggt
     1501 gagggagagg aaatcaacat tgtctacggg gatgtggatt ttgcgaattt aacttccgat
     1561 gatgacggcg gcgtgtttgt ggagttgccg gtctttgagg ggcaggaatc gtttgaagtg
     1621 acggcgaaaa atcagaccac gggccaatcc tcgcaaacaa aaaccgaggt tgaaaaaaca
     1681 cggcaaccgc taaagtcatt tgccgtgaaa accgcgtttg tgaaatggca aaaagcgaaa
     1741 aataaaaatc aagaatttac cattgccgga caatttgagt tgccgcaaga tcaatcaaaa
     1801 acggcgctgg aagaaaagct aaagctaaag ttgacgtttc ccgacggcaa aaccgctacc
     1861 gcggatttga acttcaagga aaacaatata ttttggaaat acagcagttt ggcggaaaat
     1921 caaaatctga aaatcagccg cgcggttgtc tattggtcgc ccgagaaaaa gttttgcagc
     1981 aacagcaaaa tttgcgctgt cgcgctcaaa aacaaaaact ggttctactt gcaaggcgaa
     2041 ctggaaaacc ttggcctgga aatcggcaaa accgacaaaa tggaaattga gattctttta
     2101 acggcaaaga ccgttgattt ggttggcggg caaaagttgc caatgggaaa agtcggcaat
     2161 caaaggattt atcattcttt cggtttttgg aatttttggc cgcaaaattt ggaaagcttc
     2221 tataaatgaa aaatatgaac aaaaaaatca aaagagttct gacaatcatc ggtttgattc
     2281 ttatcattgt cataatcggg atagcaattg caatgtttat gatgtttaaa gaattaaaag
     2341 gggtggttac ttcgtggtca ccgtcgggat tggacgctct gccgtgcgag ccaaaaagcc
     2401 aggttgagct ggggtcgccg tatcgcatta atggtcaagt tgctgaattt accacaaatg
     2461 gaggcaaatt atatatcact gccaagcggt ttccgcacag cggattttca ggatcgtcgc
     2521 ttggctccac ggctattttt atgggcaata gctccgcttt gcccgaatat gattcgcaga
     2581 gagatattac gacgaatacg gtaaaaaata tcagtctttc tgaaacgaaa tacgttgagt
     2641 ttgaattgcc ggcgaggagg tattggcttt ggtcaagttc cggcggggac attgtgattt
     2701 acagctgtga ggagggcggg gtgagcgatc ccaagccggt gtggcggtga agcggactgg
     2761 atcaaatttt tcagagggtt agaccctctg aatttttcaa cggcgggaag gctgatcaac
     2821 cggcagatag atctgctggc caaagaaatc caactgatgg ccaaacgggg aaccgtcggc
     2881 aaagaagttg cccaagcgtt attgagcgga ttggagcggg tgaggccaaa gtgaaaatca
     2941 aaaactaaca aaacaatatg acaaaaaagc aaaaaatatg gctggcggtt tttttggcga
     3001 tgtttctggt gccggagatt ttgtgg
//
LOCUS       DAOQWE010000104         2972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_35529_length_2972_cov_7.931436, whole genome shotgun sequence.
ACCESSION   DAOQWE010000104 DAOQWE010000000
VERSION     DAOQWE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35529_length_2972_cov_7.931436"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1001)
                     /gene="trmD"
                     /locus_tag="PKK37_03575"
     CDS             complement(264..1001)
                     /gene="trmD"
                     /locus_tag="PKK37_03575"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNR81492.1"
                     /translation="MIHFDFITIFPEIIRPYFEESLFKKAREKKLIKIRVHNLRDFAP
                     DKHHTVDDKPFGGGFGMVMKVDVWHQAIAKITKLKIAGKKLKPANKDTAVIMLTPRGK
                     TFNQKMAARLAKFRQIVFVCGRYEGVDERVAKNIATHQISVGDYDLMGGELPAMAIAE
                     AVSRLVPGVIGKPDFLAARSGKDGFFESAQYTRPEIYSPAKGINWKTPQELLSGDPKK
                     INAWRQKHGKSIGRFTKKQKSPGVEPL"
     gene            complement(1193..1513)
                     /locus_tag="PKK37_03580"
     CDS             complement(1193..1513)
                     /locus_tag="PKK37_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNR81493.1"
                     /translation="MENKNDQEFLEFLIKSLVDHPEDVKVSRTVDEMGVLLSLKVNAE
                     DMGQIIGRSGSTARAIRNLVRIIGLKNHARVNLKIEEPEGGRRAPRAEEKPSRDDDED
                     DLKF"
     gene            complement(1741..1977)
                     /gene="rpsP"
                     /locus_tag="PKK37_03585"
                     /pseudo
     CDS             complement(1741..1977)
                     /gene="rpsP"
                     /locus_tag="PKK37_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(2155..2847)
                     /gene="rnc"
                     /locus_tag="PKK37_03590"
     CDS             complement(2155..2847)
                     /gene="rnc"
                     /locus_tag="PKK37_03590"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNR81494.1"
                     /translation="MKDFSKLEKKLGVVFKNRDLLTQAFIHRSYLNENPQINLGNNER
                     LEFLGDAVLELAVTEHLYLAYPDKSEGELTSWRAALVNTKMISSAAGRMGFGDFLLLS
                     RGEAKEEGKSRQYILANTFEAFIGALYLDRGNEACRSFIKKNLLGELPEIIAKKLYKD
                     AKSHFQEMAQEVVKITPVYRVLDERGPDHAKYFTVGVYLGNELVAQAGGYSKQEAEEE
                     TAKAALAAKNWQ"
BASE COUNT          710 a          779 c          628 g          855 t
ORIGIN      
        1 tattgggtgg taagttcttg tacaatttaa cggcgtgcgc tttttcttct tgcgctaatc
       61 cttccatatt ctctttaatt tccgatagca tgtccattat ttcttttttt tcaccaccct
      121 ctattgattc taacatgttt attttgccaa aagttattgg ctttcggcaa ttgattattt
      181 tgctttatga tatgaaataa tatcgcactt tgcccctctc cgcaagattg gaacagctat
      241 caggggttca acccctgaca aatttacaga ggttcaaccc ctggagattt ttgcttcttg
      301 gtaaatctgc cgattgattt gccgtgtttt tggcgccagg cgttgatttt tttgggatcg
      361 ccggaaagca attcttgggg ggttttccaa ttgattccct tggcaggcga atagatttcc
      421 gggcgggtat attgcgcgga ttcaaaaaag ccgtccttgc cgctgcgcgc cgccaaaaaa
      481 tcgggcttgc cgatcacgcc gggaacgagg cggctcaccg cttcggcaat cgccatcgcc
      541 ggcaattctc cgcccatcag atcatagtcg ccgaccgaaa tttggtgcgt ggcgatattt
      601 ttcgccaccc gttcgtccac cccctcgtag cggccgcaga caaaaacgat ctgccggaat
      661 tttgccagcc tcgccgccat tttttgatta aacgtttttc cgcgcggcgt gagcataatt
      721 acggccgtgt ccttgtttgc cggtttcagt tttttgccgg cgattttcag ttttgtgatt
      781 ttggcaatcg cttggtgcca gacatcaact ttcatcacca tgccaaagcc gccgccaaac
      841 ggcttgtcgt caacggtatg gtgcttatcc ggcgcgaaat cgcgcaaatt gtgaacccgg
      901 atttttatca atttcttttc ccgcgccttt ttgaaaagcg attcctcaaa atagggccgg
      961 atgatttccg gaaaaatggt gatgaagtca aaatgaatca tacaatcatt taatcataaa
     1021 aacataaaat aacaaactaa aagaaaacaa cttgcattca atcttttgcc aaggtctaac
     1081 cctcgcattg gcgagggata gacccttaaa acgcagggat acgcttccca aataagtgac
     1141 tgcaaaagaa agaccccggc gaatgcgggg tctttctttt ttgagtttaa aactaaaact
     1201 tgagatcgtc ttcatcgtcg tcgcgggacg gcttctcttc ggcgcgcggc gcgcggcggc
     1261 cgccttccgg ctcttcaatc ttcaaattga cgcgcgcgtg gttcttcaag ccgataatgc
     1321 gcaccagatt gcgaatcgcc cgcgcggtgc taccgctgcg gccgatgatc tggcccatat
     1381 cttccgcgtt gactttcagc gagagcagaa cgcccatctc gtcaacagtg cggctcacct
     1441 tcacgtcttc gggatgatcc accagcgact tgatcaagaa ctcaagaaat tcctggtcgt
     1501 ttttattttc catagatatc gcgtatcatt cattcaaaca aaattcttcg acctttcagg
     1561 catcattctc aacgcctatt cataaatgtg atacccgtat accgccatta tattccaaat
     1621 caaaaatcgt tgtcaaaccg ccggcgcggc ggccggagac gcttccttta ccgaagccgc
     1681 cggttcggcg gcgccttctt tcttcttgga tttggcgtga accggaattt tctttccctc
     1741 catcaccttc tcgccgatga gaatgtttct gaccgtcggc gacggctggg cgcctttctt
     1801 catccaatcg gagatctttt cccggtcaag cttgatctct ttggtaacgc gattgacaaa
     1861 accgacttcc tcgcaaaact tgcccgcgga cgccgaaacc cgcttatcgg ccaccaccac
     1921 ccgaaacgac ggctggttct ttctgccgat acggcgcaat ctgatagtta acataatttg
     1981 gtctatggtt agtttaagtt tgttttaccc ttaatgtata acacattttt tggcatattg
     2041 caatcgccgc gaaggcaacc actcgctagg cctaaaattc ttttactagg gtctaaccct
     2101 cgcattagcg agggatagac ccttaaaaat acatacctct tctcttgaaa ttggctattg
     2161 ccagtttttc gccgccaacg cggccttggc ggtttcttct tcggcctcct gctttgaata
     2221 cccgcccgct tgcgccacca attcgtttcc caaataaacc ccgacggtga aatatttggc
     2281 atgatccggc ccgcgttcgt ccaaaacgcg ataaaccggc gtgatcttaa cgacctcctg
     2341 cgccatttcc tgaaaatgcg atttggcgtc cttgtataat tttttggcga taatttccgg
     2401 cagttcccca agaagatttt ttttgatgaa actgcggcac gcttcattgc cgcgatccag
     2461 ataaagcgcg ccgataaagg cttcaaaggt gttggccaaa atatattgcc gcgactttcc
     2521 ttcttctttg gcctccccgc gcgaaagcaa aagaaaatcg ccgaatccca tccgcccggc
     2581 cgccgaagag atcattttcg tgttcaccag cgccgcccgc caactggtga gctctccttc
     2641 ggacttgtcc ggataggcaa gataaagatg ctcggtcacc gccagctcca gcaccgcatc
     2701 gcccaaaaat tccaatcgtt cgttgttccc caaattgatt tgagggttct cgttcagata
     2761 ggaacgatgg ataaacgcct gcgtgagcaa atcccgattt ttgaaaacaa cgcccagttt
     2821 tttttcaagc ttggaaaaat ccttcatcgt tacaaattga aaatgtaaaa atcaaaaatc
     2881 aaaatgacaa ttaaaaatga taaaaattat ggaattaaaa tttttaattt taagttgtca
     2941 ttttcaattt tgatttttta attttgaatt at
//
LOCUS       DAOQWE010000105         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_35674_length_2964_cov_2.443795, whole genome shotgun sequence.
ACCESSION   DAOQWE010000105 DAOQWE010000000
VERSION     DAOQWE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35674_length_2964_cov_2.443795"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="PKK37_03595"
     CDS             <1..1849
                     /locus_tag="PKK37_03595"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81495.1"
                     /translation="FLVDRLELEDQAQKAFTQYFGKDFIVATYKKTRDSWANASVVVS
                     TIQTFLAGDRYKKEFSPIDFELVISDEAHRSIGGNSRAVFEYFVGYKLGLTATPKNYL
                     KGIGSGESDNQRDYERRLLLDTYKTFGCEPGNPTFSYDLIQGAKDGYLVQPILIDART
                     EITAELLSKNGYAVHRIAEDGQNIDEVFDERHYERKFFNEETNIAMCKAFIDNGLFDP
                     VTEKCGTPLFGKSIVFCVSQKHATRITNILNKLAFNRWPELYGESDFAMQITSQVKNA
                     QQMTVNFANNRLGGRSKKPENYDTSKTRAAVTVGMMSTGYDCQDLLNIALMRPVFSPT
                     DFVQIKGRGTRKYLFELENCGDNGKIAVSKNKFKFFDFFATCEYFENEFPYDEKIKLP
                     RIKLIADDGIVSDKKTANLEYEDENGQKIFEGPIDLAASDELESLIETPVGDQGMRID
                     REGFKRAVDEDVLGNNTLKNLWENGDTEQAEEYAQKHIFDKPKYFLNLEKIRKIFGVD
                     RRISVKEFLQFVFGDKKEFESKDALLESEWEKFIEINPVDQKHYEVVKMFFKAYITDP
                     VVREIITSRQTQRFNFESSFNFEEYQQLNGYKTIVPQYIRDYAYQLTNL"
     gene            1851..>2964
                     /locus_tag="PKK37_03600"
     CDS             1851..>2964
                     /locus_tag="PKK37_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR81496.1"
                     /translation="MNTEIKRHIDGARQVLVGVVPNPTSQIEQITNALIYKFMDDMDQ
                     AAIRQGDRPAFFTKDLERYAWTRLMDARIGNQERMNLYSEALVKFSQAAQLPDLFRSI
                     FKSAFLPYRSPETLGLFLKEINFFDYSHPEELGNAYEYLLSIMSSQGDAGQFRTPRHI
                     IDFIVDVVNPTKNDKVLDPACGTGGFLVKSYQHILELLNKKDTNGNNPKPLTPDERKK
                     LMNNFEGYDIDPAMVRISQVNMYLHQFKNPKIYQYDTLSNEDRRSNKFDVILANPPFM
                     SPRGGIKPHSKFSVQSSRSEVLFVDYIMNHLRPKGRAGIIVPEGIIFQSGASYKQLRK
                     NLVEDGLYCVASLPSGVFAPYSGVKTSILFFDNEIAK"
BASE COUNT          842 a          677 c          696 g          749 t
ORIGIN      
        1 gttcttggtt gaccgcttgg agcttgaaga ccaggcgcaa aaagctttca cgcaatattt
       61 cggcaaggac tttatcgttg cgacctataa aaaaacgcgc gattcctggg ccaatgccag
      121 tgtggttgtt tccacgatcc aaacattttt ggcgggtgat cgttataaaa aagaattttc
      181 acctatcgat tttgaacttg tcatttccga tgaagcgcat cgttccattg gcggcaacag
      241 ccgtgcggtg tttgaatatt ttgtcgggta taaactcgga ttgaccgcga cgcccaaaaa
      301 ttatcttaaa ggcatcggtt ccggcgaatc ggacaaccag cgcgattacg agcgccgctt
      361 gcttcttgat acttacaaga catttggttg cgagccgggc aatccgactt tttcctacga
      421 tctgatacaa ggagccaaag acggttattt ggtccagccg attcttattg acgcgcgcac
      481 cgaaatcacc gccgaacttc tttcgaaaaa cggttatgcc gtgcatagaa ttgccgaaga
      541 cggccaaaat attgacgagg tttttgacga gcggcactac gagcggaagt ttttcaacga
      601 ggaaacgaat atcgcgatgt gcaaagcgtt cattgacaac gggctttttg atcccgtaac
      661 tgaaaagtgc ggcacgccgc tttttggcaa atcaattgtt ttttgcgttt cgcaaaagca
      721 tgccacgcga ataaccaaca ttctaaacaa gcttgctttc aacaggtggc cggaattgta
      781 cggcgaatcg gattttgcga tgcaaattac atcgcaagtt aaaaacgccc agcagatgac
      841 ggtgaatttc gccaataacc gtttgggcgg ccggtcaaaa aagcccgaaa attatgatac
      901 aagcaaaacc cgggcggcgg taactgtggg gatgatgtcc accggctacg attgccagga
      961 cttgcttaat atcgcgctga tgcggccggt tttcagcccg accgattttg tgcaaatcaa
     1021 gggccgcggc acgagaaaat atctgtttga attggaaaat tgcggcgata atggaaaaat
     1081 cgcggtttcc aagaataaat tcaagttttt cgatttcttc gcgacttgcg aatattttga
     1141 gaacgaattt ccttacgacg aaaaaataaa actgccgcgg ataaaattga ttgccgacga
     1201 tggaattgtt tccgataaga aaaccgccaa tcttgaatac gaagatgaaa acggccaaaa
     1261 gatttttgaa ggcccgattg atcttgccgc gtccgatgaa ttggaatcgc ttattgaaac
     1321 tccggtgggc gatcagggaa tgcgcatcga tcgggaagga ttcaagcgtg ctgtggatga
     1381 ggatgtatta ggtaataata cgctcaagaa cttatgggaa aacggtgata cggaacaggc
     1441 tgaagaatac gcgcaaaagc atatttttga taagccaaaa tatttcctta atctggaaaa
     1501 aatccgtaaa atttttggcg ttgatcgccg gatttccgtt aaagaatttt tgcaatttgt
     1561 tttcggcgac aaaaaagaat ttgaatcaaa agatgcgctt ttagaatcgg aatgggaaaa
     1621 attcattgag ataaacccgg ttgaccagaa gcattacgaa gtggttaaaa tgttttttaa
     1681 agcctacata accgacccgg tagtacgcga aataatcacc agccgccaaa cccagcggtt
     1741 caattttgaa tcatcgttta attttgaaga atatcaacag ttaaatggct ataaaaccat
     1801 tgtgcctcaa tacatccgcg attacgccta ccagttaact aacttataaa atgaacacgg
     1861 aaataaaacg ccatattgac ggggcgcggc aggtgttggt aggagtggtg cccaatccca
     1921 cttcccagat tgagcaaatc accaatgccc tcatttacaa gtttatggac gatatggacc
     1981 aggccgcgat caggcaaggc gaccggcccg cgtttttcac caaagacctg gaacgctacg
     2041 cgtggactcg gctgatggac gctcgcatcg gcaaccagga acgaatgaat ttgtattccg
     2101 aagcgctagt taagttttcc caggccgcac aactgcccga ccttttccgc agcatattca
     2161 aatccgcgtt tttgccgtat cgttcgcccg aaactttggg cttgtttttg aaagaaataa
     2221 atttttttga ttattcccac cccgaagaat tgggcaatgc ttacgaatac ttgctttcaa
     2281 taatgtcgtc ccagggcgac gcgggccaat tccgcacccc gcgccatata attgatttca
     2341 tcgttgatgt cgtgaatccc accaaaaacg acaaagtgct tgatccggcc tgcggcacgg
     2401 gcggattttt ggtgaaatcc taccagcaca ttttggaact gctcaataaa aaagacacta
     2461 acggtaataa tcccaagccg ctcactcccg acgaacgcaa aaagctgatg aacaattttg
     2521 agggttacga tatcgacccg gcgatggtgc gcatttcaca ggttaatatg tatctgcacc
     2581 agttcaagaa tcccaaaatt taccaatacg acacgctttc caatgaagac cgccggagta
     2641 ataagtttga cgtgattctg gccaatccgc cgtttatgtc gccgcggggc ggcattaagc
     2701 cgcacagcaa attcagcgtg caatccagcc gctcggaggt tttgttcgtt gactacataa
     2761 tgaaccactt gcggccaaaa ggccgggccg gcatcatcgt gcccgaaggc attattttcc
     2821 aatccggcgc cagctataaa cagttgcgca aaaatctggt ggaagacggc ctttattgcg
     2881 tggccagttt gcccagcggc gtgttcgcgc cttatagcgg tgttaaaacc agcattttgt
     2941 tttttgacaa tgaaattgcc aaac
//
LOCUS       DAOQWE010000106         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_35921_length_2949_cov_8.658950, whole genome shotgun sequence.
ACCESSION   DAOQWE010000106 DAOQWE010000000
VERSION     DAOQWE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35921_length_2949_cov_8.658950"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKK37_03605"
     CDS             <1..185
                     /locus_tag="PKK37_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81497.1"
                     /translation="FPPLAGMRAWRNWQTRTVQGRVSNIMEVQPLLPAPINCNFVINY
                     HRLPATAGAVLKINAQ"
     gene            175..2115
                     /locus_tag="PKK37_03610"
     CDS             175..2115
                     /locus_tag="PKK37_03610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNR81498.1"
                     /translation="MHNNPINPGGGRAKKPAKVPAQSFDGALEAILGALPRPKKTQDN
                     SGADSAPAARQNRRPRGRGGKRFRTAPRQNQVQSQNHPQPQSRQKDDGRLRVIPLGGL
                     GEVGRNMTLLEYQNSILIMDMGFRMPGEDMPGIDYIIPNIDYLKNKVKNIVGAVFTHG
                     HYDHIGAVPYIIEKIWHPGFRMIASPLTRAIILKRQDDFPSCPKLDIESADDGGRFQL
                     GPFEVEFFRMNHNIPDNFGIFVKTPVGSVINTSDFKFDDHPINDKPVDFVRLRAIGDR
                     KILLLLCDSTGAEEPGHSLSEQEIFENLEKIFRDAKGRIIAATFGSLLNRVQQMIHLS
                     EKYGRKVAIDGYSLKNNVEVCRQMKYIRAEKGTFVQLGEIAGLPDEQTTVICTGAQGE
                     ADAALMKIANREHRFLRFKKNDSVIFSSSVIPGNERTVQNLKDNILRQGVKVYHYKMM
                     DIHAGGHAKQEELKEMTRLMHPKFFMPIHGQYSMLAANAELAVIAGVPEQNILVAENG
                     QIVSLSPDDMVLEKETAPASFVMVDGLGVGDIGEVVLRDRQVLSHDGMFVIVAVVDRL
                     TGKVKGSPDIISRGFVYLRESKDLLKETRTRTVEIIGRAVSNNGAVNWNHVKEEIRGK
                     IGDFLFAKTERKPMILPVIIEI"
     gene            2234..>2949
                     /gene="trpS"
                     /locus_tag="PKK37_03615"
     CDS             2234..>2949
                     /gene="trpS"
                     /locus_tag="PKK37_03615"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNR81499.1"
                     /translation="MKVFSGIRPTGDIHLGNYFGAIKQWIDLQNQNDCVFCVVDLHAI
                     TTPYNPKKMPAQIIGLAADYLAAGLDPDKCVLFVQSHVKEHAELAWLLGTITPMGELK
                     RMTQFKDKSKQHPEYVNAGLFNYPVLMAADILLYKAGGVPIGKDQQQHVELTRAICRY
                     FNGKFGNILKEPKEIFSEGQKIMSLAEPAKKMSKTGNSKGCIGLFEDPESIRKKIMAA
                     VTDTQKTIKYDPKKKPGIANL"
BASE COUNT          807 a          785 c          723 g          634 t
ORIGIN      
        1 atttcccgcc tttggcgggc atgcgggcat ggcggaattg gcagacgcgc acggttcagg
       61 gccgtgtgag taacatcatg gaggttcaac ccctcctgcc cgcaccaata aattgtaact
      121 tcgtaataaa ttatcaccgc ttgccggcaa cggcgggcgc ggttctgaaa ataaatgcac
      181 aataatccga taaatcccgg tggcggacgg gcaaaaaaac ccgccaaagt tccggcgcaa
      241 tcttttgacg gcgcgctgga ggcgattttg ggcgcactgc cccggccaaa aaaaacccaa
      301 gacaactccg gcgccgattc cgcaccggcg gcgcggcaaa accgccggcc gcgcggccgc
      361 ggcggcaagc gttttcgcac cgctccgcgc caaaaccaag ttcaaagcca aaaccacccc
      421 cagcctcaaa gccgccaaaa ggacgacggg cgcttgcgcg tcattccttt gggcggcctg
      481 ggagaagtgg gccggaatat gacgcttttg gaatatcaaa attccatctt gattatggat
      541 atgggttttc gtatgcccgg tgaagatatg cccggcattg actacatcat tcccaatatt
      601 gattatttga aaaacaaggt caaaaatatc gtcggcgcgg ttttcaccca cggacactat
      661 gaccacatcg gggctgttcc ctatatcatt gaaaaaatct ggcatccggg attccggatg
      721 atcgcctcgc cgctcacgcg cgccatcatc ctcaagcggc aagatgattt tccttcctgc
      781 ccgaagctgg acattgaatc cgccgacgac ggcgggcgct tccaactggg cccctttgaa
      841 gtggaatttt tccgcatgaa ccacaacatt cccgacaatt tcggcatttt cgttaagacg
      901 ccggtcggta gtgtgatcaa cacttcggat tttaaatttg acgaccaccc catcaacgac
      961 aagccggtgg atttcgtccg cctgcgcgcc atcggcgacc gcaagatatt gctccttctt
     1021 tgcgactcca ccggcgccga agaaccgggc cattcccttt ccgaacagga aatctttgag
     1081 aatctggaaa aaatctttcg ggatgccaag gggcgcatca tcgcggccac tttcggatca
     1141 ttgctaaatc gcgtccagca aatgattcac ctttcggaaa aatacggccg gaaagtcgcc
     1201 attgacggct attcgctgaa aaacaacgtt gaagtttgcc ggcagatgaa atacatccgc
     1261 gccgaaaaag gcacctttgt tcagcttgga gaaatcgccg gcttgcccga cgaacagaca
     1321 actgtcatct gcaccggcgc gcagggcgaa gccgacgcgg cactgatgaa aatcgccaat
     1381 cgcgagcacc gctttttgcg cttcaagaaa aacgactcgg tgattttttc ctcatcggtg
     1441 atcccgggca atgaacgcac cgtgcaaaat ttaaaagaca acatcctgcg ccagggagtg
     1501 aaagtctatc actataaaat gatggatatt catgccggcg ggcacgccaa gcaagaggaa
     1561 ttgaaagaaa tgacgcgctt gatgcatccg aaattcttta tgcccattca cggacagtac
     1621 tcaatgctgg cggccaacgc cgagctggcg gtcatcgcgg gagttcccga acaaaacatt
     1681 cttgtggcgg aaaacggaca gatcgtctct ctgagccccg atgacatggt ccttgaaaaa
     1741 gaaaccgcgc cggcaagctt cgtgatggtg gacggattgg gggtgggcga catcggcgaa
     1801 gtggttttgc gcgaccggca agttttgtcg cacgacggta tgtttgtgat cgtcgcggtg
     1861 gttgaccgcc tcaccggcaa agtgaagggg tctcccgaca tcatctcgcg gggattcgtc
     1921 tacctgcggg aatccaaaga cctgctcaaa gaaacgcgca cccgcaccgt ggagattatc
     1981 ggccgcgcgg tttcaaacaa cggcgcggtc aattggaacc acgtcaagga agaaatccgc
     2041 ggcaaaatcg gcgattttct tttcgccaaa accgaaagaa agccgatgat attacccgtc
     2101 atcatagaaa tctaaaaaat tgaaagtgta aaaatcaaaa atcaaaatta aggaataatt
     2161 tatatatcgt tgaaattttt tgcaaaaatt tcaactccaa aatttttcat tttgattttt
     2221 caattttaaa tttatgaaag tattctccgg aattcgcccc acgggcgaca ttcacttggg
     2281 aaattatttc ggcgcgatca agcaatggat tgatttgcaa aaccaaaacg actgcgtttt
     2341 ttgcgtggtt gatttgcacg cgatcaccac gccctacaat cccaagaaaa tgccggcgca
     2401 aattatcgga ttggccgcgg attatcttgc cgccggcctt gatccggaca aatgcgtttt
     2461 atttgtgcaa tcgcacgtaa aagaacatgc cgaactggcc tggcttttgg gcaccatcac
     2521 gcccatgggc gaattgaagc gaatgacgca attcaaggac aaatcaaaac agcatcccga
     2581 atatgtcaac gccgggctgt tcaattatcc ggtgctgatg gctgctgata ttttgctgta
     2641 taaagccggc ggcgtgccca tcggcaaaga ccagcaacaa cacgtggaac ttacgcgcgc
     2701 gatctgccgg tatttcaacg gcaaattcgg caatatcctg aaagaaccga aagaaatatt
     2761 ttccgaaggc caaaaaataa tgtcgctggc cgaaccggca aaaaagatgt ccaaaaccgg
     2821 caattccaaa ggatgcatcg gcctttttga agatcccgaa tcaatccgca aaaaaataat
     2881 ggccgcggtc accgacaccc aaaaaacaat caaatacgac cccaagaaaa aaccgggcat
     2941 cgccaatct
//
LOCUS       DAOQWE010000107         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_36021_length_2943_cov_8.255886, whole genome shotgun sequence.
ACCESSION   DAOQWE010000107 DAOQWE010000000
VERSION     DAOQWE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36021_length_2943_cov_8.255886"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1275)
                     /locus_tag="PKK37_03620"
     CDS             complement(97..1275)
                     /locus_tag="PKK37_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HNR81500.1"
                     /translation="MRTGIATVPLDWGRCPRWLFERMTRLGRGICVAIVEEFGPEELL
                     RRLSDPAWFQSLGCVMGFDWNSSGLTVTTMGALKEAVRGMETDLGFYVCGGKRVSRKT
                     PEETAAWGQKLGWTEEKTGTLVFASRMAAKVDSALVQDGFSIYAHNLLFTNSGKWAVI
                     QQGMNTDLQKARRYHWLSDGVNDFCENPHSGIASDARLKPLNLTAEESRENKEISAQM
                     VKLEPAAVLKNLQTAAEKVSKNMPAVANPGGSSLGKQSRLPGFSEMELNDVEFHWHPV
                     IAEKFDMKRLKKTIETAHFFAPTEFAQLLAGEGVGPKTIRALSLVAEIIYGAKPSYED
                     PARYTFAVGGKDGTPFPVDRATYDKTLAAIERGIIASRSGAKEIDAAKRRLETVTYPF
                     "
     gene            complement(1645..>2943)
                     /locus_tag="PKK37_03625"
     CDS             complement(1645..>2943)
                     /locus_tag="PKK37_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR81501.1"
                     /translation="IPLALYFYVLLTVQSFENAAMNEIEAHDFTASDALLAMVERDFP
                     AKDTANLEALQKTIDRIVANDTRLKNIRVIVKDGRDYKAVAAQNRDQVGMSIDVTPSL
                     NGESLIAYSWVNASEDVSSEIEIAGEKLRRVIKVFVGEDGEVYALAAADFSINRTLDM
                     IAAGVYQSYLIMGVIMILSLFLIVQHARLFSYVGLSKDLQQKNTAKDNFIRMATHELR
                     APVTVLTGYTDLLKEDLAGTVDADQQKYIDRMVLSVKNLSDLMADILEVSHLEQGRTD
                     FEPEIIAPDIVVKEIVDGLAAKAEQKGLKLSFDAKGFSHKIRVNPVCFKRIVTNLVEN
                     SIKYTPAGKVAVTVAAQTAKKRCAITIQDTGFGISAEGQAHLFEQFYRVKTQENAEIP
                     GTGLGLWMSREMARKMGGDIMLESIEKMGSKFFIFFPLAK"
BASE COUNT          705 a          832 c          680 g          726 t
ORIGIN      
        1 gtcagttttt caaattcatt taattccata gcaaattgat cagagtttta gagttttaga
       61 tttacatagg tgttacctat gtaaatctat gtaaatttaa aacggatagg tgacggtttc
      121 cagacggcgt ttggcggcgt caatttcttt ggcgccggac cggctggcaa tgatgccgcg
      181 ctcaatcgcg gccagggttt tgtcgtaggt tgcgcgatcc accggaaacg gcgtgccgtc
      241 tttgccgccc acggcaaaag tgtaacgcgc cgggtcttca taagacggct tggcgccgta
      301 aataatctcg gccaccagcg acagcgcccg gatggttttg ggaccaacgc cttcgccggc
      361 cagcaactgc gcaaactccg tcggcgcgaa aaaatgcgcc gtctcaatcg tctttttcaa
      421 ccgcttcata tcaaactttt ccgcgatgac cggatgccaa tgaaattcaa catcgtttag
      481 ctccatctcg gaaaatccgg gcagacggct ctgttttccc aagcttgaac cgccgggatt
      541 agcaaccgcc ggcatatttt ttgaaacttt ttcggccgcg gtttgtaaat ttttcagcac
      601 ggccgcgggc tcaagcttca ccatttgcgc ggaaatctct ttgttttccc ggctctcctc
      661 ggcagtcaaa ttcagcggct ttagccgcgc gtcgctggca atgcccgaat gcggattttc
      721 gcagaaatca ttcacgccgt ccgacagcca gtgatagcgc cgcgcttttt gcaaatcagt
      781 gttcatcccc tgctggatca ccgcccattt gccggaattg gtgaacaaca aattgtgcgc
      841 gtaaatggaa aatccgtcct gcaccagcgc cgaatccact ttggccgcca ttctgctggc
      901 aaagaccagc gtgccggttt tttcttcggt ccagccgagt ttttgccccc acgccgccgt
      961 ttcttcggga gttttgcgcg acacgcgctt gccgccgcaa acataaaatc ccagatcggt
     1021 ttccatgccc cgcaccgctt cttttaacgc gcccatcgtg gtgacggtga gtcccgacga
     1081 attccagtca aaccccatca cgcatcccag cgactggaac catgccggat cggaaagccg
     1141 ccgcaacaac tcttcggggc cgaattcctc cacgatggca acgcaaatcc cccgccccag
     1201 gcgggtcatc ctctcaaaca gccagcgggg gcagcgcccc caatccagcg gcacggtggc
     1261 aattccggtt cgcatatgag tccactctaa cgcaaaactg acttggtagc cagttttgcg
     1321 ttagagcaat tttcaatttt cagttttcaa ttttcaaaca aatgccaaat tgcaaaacca
     1381 gagcacggaa ttataaaaaa cggtgctatc taccagagcc atggcaaaaa ttaccaaaac
     1441 ggcagtagcg tatttttgca ttttaagggt ctatcccccg tgcctgtccc gaggtaatcc
     1501 gagggataat cgcgggggtt agaccctggc aaagaattgc aagcacaaaa ttaaaagggt
     1561 ctaaccctcg cagtaacgag ggatagaccc ttttaatttt agacccagtg aaaattggaa
     1621 attgataatt gaaaattatt ttgattactt ggccagcggg aaaaagatga aaaatttgga
     1681 gcccattttt tcaatgcttt ccagcataat gtcgccgccc attttgcgcg ccatttcgcg
     1741 cgacatccac aaccccaatc ccgtgccggg aatttcggcg ttttcttggg ttttcacgcg
     1801 ataaaattgt tcaaacaaat gcgcctgccc ttcggccgaa atgccaaaac cggtgtcctg
     1861 aatggtgatg gcgcaccgtt ttttggcggt ttgcgccgcc accgtcaccg ccacttttcc
     1921 cgccggcgtg tatttgatgg aattttccac caaattggtg acaatccgtt tgaaacacac
     1981 cggattgacg cggatcttgt gggaaaagcc tttcgcgtca aaagaaagct tcagtccttt
     2041 ttgttcggct ttggccgcca atccgtcaac aatttccttg accacaatat cgggcgcgat
     2101 gatctcgggt tcaaaatcgg tccgcccctg ctctaagtgc gacacttcca aaatgtcggc
     2161 catcagatcg ctcaggtttt tgaccgataa taccatccgg tcaatatatt tctgttggtc
     2221 cgcgtccacc gtgccggcca aatcttcttt gagcaagtcc gtgtaaccgg taagcaccgt
     2281 caccggcgcg cgcaattcat gggtcgccat acggataaaa ttatctttgg cggtattttt
     2341 ttgttgcaga tccttagaca agccgacata ggaaaacagc cgcgcgtgct gaacgatcaa
     2401 gaacaaactc aaaatcataa taactcccat aatcaaataa ctttggtaga cgccggcggc
     2461 gatcatatcc agcgtccgat tgatggaaaa gtcggccgcc gccaaagcgt agacctcgcc
     2521 gtcttcgccg acaaacacct taatcacccg ccgcaatttt tccccggcaa tctcaatttc
     2581 cgacgacaca tcctcgctgg cattaaccca gctgtatgcg atcagcgact cgccgttcag
     2641 agacggagta acgtcaatgg acatccccac ttgatcgcgg ttctgcgcgg cgactgcttt
     2701 atagtcccgg ccgtccttga caatcacgcg gatatttttc agccgcgtat cgttggcaac
     2761 gatccgatca atcgtttttt gcaacgcttc aagattcgcc gtgtccttgg ccggaaaatc
     2821 acgctccacc atcgccaaaa gcgcgtcgct cgcggtgaag tcatgcgctt caatttcatt
     2881 cattgccgca ttctcaaacg attgcaccgt caagagaacg taaaaataaa gcgccagcgg
     2941 aat
//
LOCUS       DAOQWE010000108         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_36372_length_2926_cov_3.889586, whole genome shotgun sequence.
ACCESSION   DAOQWE010000108 DAOQWE010000000
VERSION     DAOQWE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36372_length_2926_cov_3.889586"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKK37_03630"
     CDS             <1..508
                     /locus_tag="PKK37_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81502.1"
                     /translation="ECEPGLSEANCNEFCKIVVEKKPEIQDRCGTADGGKDLTDPGVS
                     FDIVRAPYLIERRDSNGSRFFSSYYTCDEVCAARGQKCIGVGLTNVAVNRCVSVVHDI
                     GLNCELSGNLQGGSGCSDTYIKQAETCLEYSSPCKPEWEPCRKDSTTAKNEEYPAFSV
                     GETACYCQ"
     gene            535..1290
                     /locus_tag="PKK37_03635"
     CDS             535..1290
                     /locus_tag="PKK37_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81503.1"
                     /translation="MEQEYINYEEGGKEGKPSFGVDWVRLALFAVAGVLIIAIITLLI
                     FAKTGGGKAPAEKNQPAGDENVVSENQSEAGFAAPAKEYNYQAPSQESCRGLPSEEEI
                     QLCLDNVAADKAIQGKNLSACYEIKNESLRFACVSRVARGVQDADLCLETSDSLAQYR
                     CVAMIASEKNDSALCDKLSSSEDRQNCQDQVAAFSIAQDGDKDSLYKCRKLKSQEYID
                     LCFFHSFVNKFGGDCGAVPQNYRQECLDVFAKE"
     gene            1305..2309
                     /locus_tag="PKK37_03640"
     CDS             1305..2309
                     /locus_tag="PKK37_03640"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81504.1"
                     /translation="MAENSSNDPQKTDQSKKRRRIIIFFAALLALLAVVWGIVAWIQN
                     DAARNKPPQRVYNYIAPSREDCLKMKNSEDADFCLDRVIAAEAVNDGDFYRCAEIKDE
                     WVRDDCRLAVARNLGDEKLCEIMGSKFGKTRCLSIVGIVKKDTAVCDLFGDGEPYEKQ
                     ECVDRVMSFIIAEDGDKETISKCADLKTLEYEKLCLMNSYENKFGGDCAAVPQAFRQY
                     CLAQAIISGDPNFDDCNEIDMKDDRDTNNYRNFCYKVAQMGHIEAAKVDSDGDGVNDA
                     NELFMNLDPYNADVDGDGLTDGEEWIIYGTNPASRDSDGDGVGDYEQAMSEKLPDGGE
                     "
     gene            2359..>2926
                     /locus_tag="PKK37_03645"
     CDS             2359..>2926
                     /locus_tag="PKK37_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNR81505.1"
                     /translation="MDKINSLLQRHNEWRSSCINLIASENVMSPACKKLYVSDLMHRY
                     AEGTPRKRYYRGLEYIDQIEELVQGEFKKHFGAGFCDARPISGTLANYAAFAAISSRG
                     DKILSLGVEHGSHVSHEKAGAAGTLGLEVGWLEYNDDCTMNAEKSCAKILEYKPRFIV
                     IGGSVILFPQPIKELREACDKVGAKIVYD"
BASE COUNT          782 a          610 c          841 g          693 t
ORIGIN      
        1 agaatgcgag cccggacttt cggaggcaaa ctgcaacgaa ttctgcaaga ttgtcgttga
       61 gaagaaaccg gagatccagg atcgttgcgg caccgccgac ggcggcaagg acctcaccga
      121 tccgggcgtg agttttgata ttgtgcgggc gccatactta attgaacgaa gagacagcaa
      181 cggaagccgg tttttttcca gctattatac ttgcgacgaa gtttgcgccg cacgagggca
      241 aaagtgcatc ggcgtggggc tgaccaatgt ggccgtgaac cgttgcgttt cggtggtgca
      301 cgacatcggc ctcaattgcg aactgtcggg gaatttgcag gggggtagcg gatgttccga
      361 cacatatata aagcaggcag agacttgcct ggaatattcc agtccctgca agccggagtg
      421 ggagccgtgc agaaaggatt ctacaaccgc taaaaatgaa gagtatccgg cgttttccgt
      481 gggcgaaact gcttgctatt gccaataatt taaatcgcga aagcgctcta ttttatggaa
      541 caagaatata taaattatga agagggtgga aaagaaggca aaccatcgtt cggcgtggat
      601 tgggtcaggc tggccctttt cgccgttgcc ggcgttttga taattgcgat aatcacattg
      661 ctaatttttg caaagaccgg cggcggcaag gcaccggcgg aaaaaaatca gccggcaggc
      721 gatgaaaatg tggtttcaga aaatcaatcg gaggcgggct ttgcggcgcc ggccaaagag
      781 tataattatc aagcgccgtc tcaagaaagc tgtcgtggtc ttccttccga agaggagatt
      841 caattatgcc tggacaatgt tgccgcggac aaggcgattc agggcaaaaa tttaagcgct
      901 tgttacgaaa taaaaaacga atctttgcgc tttgcctgcg tttcaagggt tgctcggggc
      961 gttcaggatg cggacctgtg ccttgagacc agcgatagcc ttgcccaata ccgttgcgtg
     1021 gcaatgatcg cttcggaaaa aaatgactcc gcgttgtgcg acaaattgag ttcttcggag
     1081 gaccggcaaa attgccaaga tcaagtggcg gcgttttcca tcgcgcaaga cggagacaag
     1141 gatagcttgt ataaatgtcg caagctgaaa agccaggaat atattgatct gtgttttttt
     1201 cattcctttg tcaataaatt cggcggggat tgtggcgcgg tgccgcaaaa ttaccgccag
     1261 gaatgtcttg atgtgtttgc gaaagaataa taatcaatta aaatatggca gaaaattcat
     1321 cgaatgatcc gcaaaaaacg gatcaaagca aaaaacgccg ccggataatt atcttttttg
     1381 ccgcgctttt ggcgttgctg gcggtggttt ggggaatcgt ggcgtggatc caaaacgatg
     1441 cggcgcggaa caaaccgccg cagagagtct ataattatat cgcgccgtcc cgggaggact
     1501 gcctgaaaat gaaaaattcc gaagatgccg atttttgcct ggacagggtg attgccgccg
     1561 aggcggtgaa tgacggggat ttttaccggt gtgccgaaat caaggatgaa tgggtgcgtg
     1621 atgattgccg ccttgccgtg gcgcgtaatc ttggtgacga gaagctgtgc gaaattatgg
     1681 gatcaaaatt cggcaaaacg cgctgtttga gcattgtggg aatcgtcaaa aaagacaccg
     1741 ccgtttgcga tcttttcggc gacggcgaac cttatgaaaa acaggagtgc gttgaccggg
     1801 tgatgtcatt tatcatcgcc gaagacggcg acaaagaaac catttccaag tgcgcggatc
     1861 tgaaaaccct ggaatatgaa aaattatgcc tgatgaattc atatgaaaac aaatttggtg
     1921 gcgattgcgc cgcggttccc caggcgtttc ggcaatattg cctggcgcag gcgatcattt
     1981 ccggcgatcc gaattttgac gattgcaatg aaattgatat gaaagatgat cgggacacca
     2041 acaattaccg caatttttgt tacaaggtgg cgcaaatggg acatattgaa gcggctaaag
     2101 ttgattcgga cggcgacggg gtgaacgatg ccaacgaatt gtttatgaat ttggatcctt
     2161 acaatgcgga tgttgacggc gacggattga ctgacggcga agaatggatt atttatggca
     2221 ccaatccggc gtcaagagac agcgacggcg acggggttgg cgattatgaa caagcaatgt
     2281 ccgaaaaatt gcccgatggc ggggaataaa ttgttccgcc ggccgggata ttccgacaga
     2341 taaacagaaa acacatttat ggataaaata aattcgttat tgcaacggca caatgaatgg
     2401 agaagttctt gtattaattt gatcgcttcg gagaacgtga tgtcgccggc gtgcaaaaag
     2461 ctttacgtgt ccgatttgat gcatcgttat gcggaaggca cgccgcgcaa gcgttattac
     2521 cgcggtttgg agtatattga ccaaattgaa gagctggtcc aaggggaatt caaaaagcat
     2581 tttggcgccg gtttttgcga tgcccggccg atttcgggga cgctggccaa ctatgcggcg
     2641 tttgccgcaa tctcttcgcg gggtgacaaa attttgtcgc tgggggtgga acatggctcc
     2701 catgtttccc atgaaaaggc gggcgcggcg ggaacacttg gattggaggt gggctggttg
     2761 gaatataacg atgattgcac gatgaacgcg gaaaaatcct gcgccaaaat tttggaatat
     2821 aaaccgcggt ttatcgttat cggcggatcg gtgattttgt ttccccagcc aatcaaagaa
     2881 ttgcgcgagg cgtgcgacaa ggtcggcgcc aaaatcgttt atgacg
//
LOCUS       DAOQWE010000109         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_37170_length_2883_cov_9.886846, whole genome shotgun sequence.
ACCESSION   DAOQWE010000109 DAOQWE010000000
VERSION     DAOQWE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37170_length_2883_cov_9.886846"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PKK37_03650"
     CDS             <1..1143
                     /locus_tag="PKK37_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNR81506.1"
                     /translation="ISHFLEHLQFKGTAKRPSEMAIFSEIDDLGGVANAFTSQEMTGY
                     YLKVQSSKFAAAFDLVADIFLNSVLPVQEIEKERGTIIEELKMFYDHPMRYIWTVWNQ
                     ALYGDQPAGWDIAGTIETVKGISRGRLLEYRDKNYAAQNTVVCISGNFDEKEGEKLAK
                     KYFGRFAAKDPAKKSAVAEKQATPQVLIYKKDTNQTQIALGVRAFNYNYPRRRALELL
                     ETVLGGMFSSRLMEEVRFKRGLVYDVHTELSTDPDTGSLAATANLDSARLDEGIKVIL
                     GEFKRIRDEKVSPRELRKAKDHYIGKNSIVLESSHAKGLFYGEQELLEERILEPEEIY
                     EEIGRVSAEDIQAAARDIFSPEKLNLAVIGPDDNRQRFEKLLENAL"
     gene            1178..2215
                     /locus_tag="PKK37_03655"
     CDS             1178..2215
                     /locus_tag="PKK37_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR81507.1"
                     /translation="MFPIKRKTVFDVSWGSLAKIGVALALGWGVYLSRDVLVMAFSGL
                     IISALFNPAVDFLERAKISRPVAVFLVYFSIFGSLGAGIYLVAPFFIAETQQLGQLFP
                     MYFEKLAPFLSGLGFAVFQSMDAFVAAVRDWLVSASSSIVGSVAAVFDGIFTMVIVIT
                     AAVFFSLENRGIKKCIVMLAAEKHEKTVLEWWQKAQAKISGWFAVRLAGMAAVGFLTW
                     LMCWALDVRYPIFLGFFAGIADIVPFIGPIATGAVIALVALIDSWQKAVLLVAIFTVI
                     QQLENNLVLPLLSQKFIAFPAILVLISLLVGEALWGLAGAILAIPMFGIAYDFVGDYL
                     IKHRRALKCES"
     gene            2217..>2883
                     /gene="rsmI"
                     /locus_tag="PKK37_03660"
     CDS             2217..>2883
                     /gene="rsmI"
                     /locus_tag="PKK37_03660"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNR81508.1"
                     /translation="MAGTLHIVATPIGNLDDITARAIKILGAADLILCEDTRVAQKML
                     ARFGIQKPRESYHQHSTLRKADYILDLLREGKNLALVSDAGTPGICDPGNQLVSAAVE
                     KFGDEIKIIPVPGASAVAAALSVCGFCADKFVFLGFPPAKNKRKKYFEELLANEYTAV
                     FYESNFRILKTLAEIDELNHDKDRRIVVCRELTKAFETIYRGLIAEVAAKIKKDPLKG
                     EFIA"
BASE COUNT          674 a          640 c          826 g          743 t
ORIGIN      
        1 atttcccatt ttttggaaca tttgcaattc aaaggaacgg ccaaacgccc gtcggaaatg
       61 gcgatttttt cggaaattga cgatctgggc ggcgttgcca atgccttcac ttcgcaggaa
      121 atgaccggct attatctcaa agtgcagtcc tcaaaatttg ccgcggcgtt tgatctggtc
      181 gccgacattt ttttgaattc cgttctgccg gtccaggaaa ttgaaaaaga acggggaaca
      241 atcattgaag aattgaagat gttctatgac catcccatgc gctatatctg gacggtttgg
      301 aaccaggcgc tctacggcga ccagccggca gggtgggata ttgccggcac gattgaaacg
      361 gtgaaaggta tttcccgcgg ccggctgttg gaatatcggg acaaaaatta cgcggcgcaa
      421 aacacggtgg tttgcatttc cggcaatttt gatgaaaagg agggcgaaaa gctggcgaaa
      481 aaatatttcg ggcggtttgc cgccaaagat ccggcaaaaa aatccgccgt ggcggaaaag
      541 caggcaacgc cgcaagtttt gatctataaa aaagacacca accaaacgca gatcgcgctg
      601 ggagtgcggg cgtttaatta caattatccg cgccgccggg cgctggaatt gctggaaacc
      661 gttctgggcg ggatgttttc ttcccgtctg atggaagagg tgagattcaa gcgcgggttg
      721 gtctatgacg tgcacaccga attgtcaacg gacccggaca ccggttcgct ggcggcaacg
      781 gcgaatttgg acagcgcccg ccttgatgaa ggaatcaaag tgattttggg cgaattcaaa
      841 agaatccgcg atgagaaagt ttcgccgcgg gaattgcgca aggcaaaaga tcattacatc
      901 ggcaaaaatt ccattgtttt ggaatcgtct cacgccaaag gattgtttta cggcgagcag
      961 gaattgctgg aggaacggat attggaaccc gaggaaattt atgaagagat cggccgggtg
     1021 tccgccgaag acatacaagc ggcggcaaga gatatctttt cccccgaaaa actcaatttg
     1081 gcggttatcg gtcccgatga caaccgccaa cgctttgaaa aattactgga aaacgcgcta
     1141 taatttgttc agaggcaaga attaaaatca aaacaaaatg tttccaataa aacgaaaaac
     1201 agtttttgac gtttcgtggg gttcgctggc gaaaatcggc gtggcgctgg cgcttggctg
     1261 gggagtttat ctgtcgcggg acgtgctggt gatggcgttt tccggattaa tcatttccgc
     1321 gcttttcaat ccggcggttg attttttgga aagggccaaa atttcccgtc cggtggcggt
     1381 ttttctggtc tatttttcaa tcttcggttc tctgggcgcc ggcatttatc tggtggcgcc
     1441 gtttttcatc gctgaaaccc agcaattggg acagcttttc ccgatgtatt ttgaaaaact
     1501 ggcgccgttt ttgtccgggc tggggtttgc cgtttttcag agtatggacg cgttcgtggc
     1561 ggcggtgcgc gattggctgg tgagtgcgtc ctcttccatc gtcggttcgg tcgccgcggt
     1621 ctttgacggt atttttacaa tggtcatcgt gatcaccgcg gcggttttct tttcgctgga
     1681 aaaccggggc attaaaaaat gtatcgtgat gctggcggcc gaaaagcacg aaaaaaccgt
     1741 gcttgaatgg tggcaaaaag ctcaagcgaa aatttcgggc tggtttgcgg tgcgtttggc
     1801 gggaatggcg gcggtgggat ttttaacttg gctgatgtgt tgggcgcttg acgtgcgcta
     1861 tccgatattt ttgggatttt tcgcgggcat cgccgatatt gtgccgttta tcggcccgat
     1921 tgcgacgggg gcggtaatcg cgctggtggc gttgattgat tcctggcaaa aggcggtttt
     1981 gctggtggca attttcacgg tgattcagca gttggaaaac aatttggtcc tgcccctgct
     2041 gtcgcaaaaa ttcatcgcgt ttccagcgat cttggtgctg atttccttgc tggtcggcga
     2101 agcgctgtgg ggcttggcgg gcgcaatcct ggcgattccg atgtttggca tcgcttatga
     2161 ttttgtcgga gattatctga tcaagcaccg gcgcgccctg aaatgcgaaa gctgatatgg
     2221 cgggaacttt gcacattgtt gccaccccca tcggcaatct ggacgacatc accgcgcgcg
     2281 cgattaaaat tctgggcgcc gcggatttga ttttgtgcga agacacgcgc gtggcgcaaa
     2341 agatgctggc gcgctttggc attcaaaaac cacgggaaag ctatcaccag cattccactt
     2401 tgcgaaaagc ggattatatc cttgatttgc tgagggaagg caaaaatctg gcgttggttt
     2461 ccgatgccgg cactcccggg atttgtgatc cggggaatca gttggtttct gcggcggtgg
     2521 aaaaattcgg cgatgaaata aaaatcatcc ccgtccccgg agccagcgcc gttgccgcgg
     2581 cgttaagcgt ttgcgggttt tgcgccgaca agtttgtttt tttgggattt ccgccggcga
     2641 aaaacaaacg caaaaagtat tttgaggaat tgttggcgaa tgaatatacc gcggtgttct
     2701 atgaatcaaa tttccggatt ttgaaaactc ttgccgaaat tgacgaattg aatcatgata
     2761 aagaccgccg cattgtcgtt tgccgcgagc ttaccaaggc gtttgagacc atctatcgcg
     2821 gtttgattgc cgaggtggcc gccaaaataa aaaaagaccc cctgaaaggc gaatttatcg
     2881 cgg
//
LOCUS       DAOQWE010000110         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_37843_length_2850_cov_10.561002, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000110 DAOQWE010000000
VERSION     DAOQWE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37843_length_2850_cov_10.561002"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKK37_03665"
     CDS             <1..248
                     /locus_tag="PKK37_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81509.1"
                     /translation="LLLYPVKNGIKTLLPEQFVFCYFPILHFVSGFKIVISDFPPPKA
                     EGWRGVPNGKVPLSKSGGRKPMRVRLSPPPPEIVISR"
     gene            296..688
                     /locus_tag="PKK37_03670"
     CDS             296..688
                     /locus_tag="PKK37_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HNR81510.1"
                     /translation="MAFFSKKQNKEEPQNIDEILAEFKKVKDKCQKLAAKVDRLEAKN
                     RQNIGKVGIVRFNPFEGFGGNQSFSLAMLDENDCGVAITSLFSRDGNRVYAKPIENGE
                     SQYALSKEEKEAIALAQNNLNLKNQNEK"
     gene            780..2291
                     /gene="infB"
                     /locus_tag="PKK37_03675"
     CDS             780..2291
                     /gene="infB"
                     /locus_tag="PKK37_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNR81511.1"
                     /translation="MAGKSDKKSISRPPVVVIMGHVDHGKTSILDYIRKTHVADRESG
                     GITQHIGASVAEFQGKKITFIDTPGHEAFSQMRRRGAKIADIAVLVVAAEEGCKPQTK
                     EAAAAIKKAGLPVIVAMNKTDLPQANVEKVKRDLEKEGLLVEDWGGNVPAIGISAKTG
                     QGINELLEMILLVAEMENLQANPDAAAAGIIIESSLDHRRGPSAAILLQEGNLSAGKV
                     IATASALGKIKFLEDSRGNKIERLLPSQPAVAIGLDAVPVTGEVFQEFESLDAAREFV
                     IGRQKEIKSGAAAAETVELQEGQKNLNLILKADVLGSLEVLKETLLQIPQNEVVLNVL
                     DGGAGEISLNDIKTARAAQAAGTTAIIGFRVKINPDAKAMAEREKIRVMNFEVIYEAI
                     EWTRGYMGRIRTPKMERKDLGKLKVLLSFWADKGRQIVGGKVIDGEVKKGVKIEVFRN
                     ENPVGQGRLINLQKNKKDIPDARKGDEVGILYEGQEKIQEGDHLIFYVQEKNI"
     gene            2269..2631
                     /gene="rbfA"
                     /locus_tag="PKK37_03680"
     CDS             2269..2631
                     /gene="rbfA"
                     /locus_tag="PKK37_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNR81512.1"
                     /translation="MFKKKTFNMSEHRIEKIESLIHQVLGEMIQREFTPPEGALVSLT
                     RIAVSGNLQEAKVYISVIPDRLGNEVVGNLQKNAWRFQEELNKKLKMRPVPKIIFAAD
                     RQAAQAQAVETIMEQLKK"
BASE COUNT          853 a          657 c          720 g          620 t
ORIGIN      
        1 ttttgttgct ttatcctgtt aaaaatggta taaaaacgct gttgcccgaa cagtttgttt
       61 tttgttattt tcccattttg catttcgttt cgggttttaa gattgtaatt tcggatttcc
      121 ctccgccaaa ggcggagggc tggaggggtg ttccgaatgg caaggtaccg ctctcgaaaa
      181 gcggcgggcg caagcccatg cgagttcgac tctcgccccc tccgcccgaa atagtgataa
      241 gcagatgaac aaaacgcaca tcttctcatc actcattaac taatttactg gtccgatggc
      301 ttttttttca aaaaaacaaa acaaagagga accgcaaaat attgatgaaa ttctggccga
      361 atttaaaaag gtgaaagaca agtgccaaaa actggccgcc aaagttgacc gtttggaagc
      421 aaaaaaccgg caaaacatcg gcaaagtcgg cattgtccgg ttcaacccgt ttgaaggatt
      481 cggcggcaat caaagttttt ccctggcaat gctggatgaa aacgattgcg gcgtggcaat
      541 caccagcttg ttctcccgcg acggcaaccg cgtctatgcc aaaccgattg aaaacggcga
      601 gtcgcaatat gccctgtcaa aagaagaaaa ggaagccatt gcgcttgccc aaaataattt
      661 aaatttgaaa aatcaaaatg aaaaatgaca atgtaaaatg aaaaatcatc aaatcaaaat
      721 tccgtaagga attttacctt aattttacat tttgattttt acattttcaa ttatcttcaa
      781 tggctggcaa atccgataaa aaatcaatct cccggccgcc ggtggtggtg ataatggggc
      841 acgtggacca cggaaaaacg tcaattttgg actatatccg caagacccat gtggccgacc
      901 gcgaatccgg cggcatcacc cagcacatcg gcgcatccgt cgcggaattt caagggaaaa
      961 aaattacttt cattgacacc cccgggcacg aggcgttttc gcagatgcgc cggcgcggcg
     1021 cgaaaatcgc cgatatcgcg gtgctggtgg tggccgccga agaaggttgc aaaccgcaaa
     1081 ccaaggaagc cgccgcggcc atcaaaaaag ccggcctgcc ggtgatcgtg gcaatgaata
     1141 aaaccgattt gccgcaagcc aatgttgaaa aggtgaaacg tgatctggaa aaagagggac
     1201 tgctggtgga ggattggggc ggtaacgtgc cggcgatcgg catttcggca aaaaccggcc
     1261 agggcattaa cgaactgttg gaaatgattc tgctggtggc ggagatggaa aatttgcaag
     1321 ccaatccgga cgccgcggct gccggtatca ttattgaatc ttctctggac caccggcgcg
     1381 gcccaagcgc cgcgatcttg ctccaagagg gaaatctgtc cgcgggaaaa gtcatcgcca
     1441 ccgcttccgc actggggaaa ataaaatttc tggaagactc gcgcggcaac aaaattgaga
     1501 gattgctccc ctcccagccg gccgtcgcga tcggattgga tgccgtgccg gtgaccggcg
     1561 aagtttttca agaatttgaa agcctggacg ccgcgcggga atttgtgatc ggccggcaaa
     1621 aagaaatcaa aagcggtgcg gcggcggcgg aaaccgttga acttcaagaa ggccaaaaaa
     1681 atttgaatct gattttgaaa gccgatgttt tgggatcgct ggaagtcctc aaagaaacct
     1741 tgcttcaaat cccccaaaat gaagtcgtct taaacgtttt ggacggcggc gccggagaaa
     1801 tcagcctcaa tgacatcaag accgcgcgcg cggcgcaagc cgcgggcacc accgccatca
     1861 tcggtttccg ggtgaaaata aatcccgatg ccaaggcaat ggccgagcgg gaaaaaattc
     1921 gcgtgatgaa ttttgaagtg atttacgaag caattgaatg gacgcgcggt tatatgggaa
     1981 ggatccgcac tccgaaaatg gaacgcaaag acctgggcaa attgaaagtt ttgctcagtt
     2041 tctgggccga taagggccgc cagatagtgg gcgggaaagt cattgacggc gaagtcaaaa
     2101 aaggcgttaa gattgaagtc ttccgcaatg aaaatccggt cggacaagga cggctgatca
     2161 atctgcaaaa aaataaaaaa gacatccccg atgcccgcaa gggagacgaa gtgggcattc
     2221 tttacgaagg gcaggaaaag atacaggagg gcgaccacct gatattctat gttcaagaaa
     2281 aaaacattta atatgtcgga gcaccgcatt gaaaaaatag aatcactgat ccaccaagtt
     2341 ctgggagaga tgatccagcg ggaatttaca ccgccggagg gcgcgctggt atcgctcaca
     2401 aggatcgccg tcagcggcaa tctccaagaa gccaaagttt atatcagtgt catccccgac
     2461 cgcctcggca acgaagtcgt cggcaatctg cagaaaaacg cgtggcggtt ccaggaagaa
     2521 ctgaacaaaa agttaaaaat gcggccggtg ccgaaaatca tttttgccgc cgaccggcaa
     2581 gccgcgcaag cccaagcggt ggaaaccatt atggaacaat tgaaaaaatg atgattttgt
     2641 attaggcttt atccatcagc ccaccactca tttaaccatt gacaatccgc ggccggttag
     2701 ccaagtagta aggcgacagt ctgcaaaact gttatacatg agtgcgattc tcataccggc
     2761 ctccaacaaa attgaaaatt gaaaaattga aatttaaaat taaggtaaaa ttccttacgg
     2821 aattttgatt ataattttta attttaaatt
//
LOCUS       DAOQWE010000111         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_38164_length_2834_cov_7.335732, whole genome shotgun sequence.
ACCESSION   DAOQWE010000111 DAOQWE010000000
VERSION     DAOQWE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38164_length_2834_cov_7.335732"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKK37_03685"
     CDS             complement(<1..387)
                     /locus_tag="PKK37_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81513.1"
                     /translation="MQFKIPKFGFKFKKRGNAAGFFGAKLSIISNHLSIINDKLSKKR
                     INYKLSIFGKKIFINYKLLIAVGVLALAATGGTLALNGFFGGGAAIPEDTMTRGLVGY
                     WSFDEGDGTVARDASGNNNHGTLTNGP"
     gene            complement(559..1059)
                     /locus_tag="PKK37_03690"
     CDS             complement(559..1059)
                     /locus_tag="PKK37_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR81514.1"
                     /translation="MGDEYENYRTAAGTIDWGKYYSEKAANDRLSIINNQSKKSKPLE
                     ELSIYRLADDFSDQVWDLIVKWNFFEKKTLGDQFVRAADSIAANIAEGYGRYFFGEYR
                     VFLYYARGSLYECKLWLEKTRKRSLVSDDLYQEMQKMLEELAVEINKVIKIVKGEQAK
                     WKGRSW"
     gene            complement(1158..2123)
                     /locus_tag="PKK37_03695"
     CDS             complement(1158..2123)
                     /locus_tag="PKK37_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81515.1"
                     /translation="MSSETINKIAHFAKYHNGFVVGLMLAFFGGAAIFAASPAAREAV
                     LGEEIITESGVDNSAILAADLDNFDFEIKINDVAEDENNYYVDYSFRTLEIQDNIWQE
                     AQRRSSMAIDKLSLAGKDLGLYVQEQLVQIAQNEQNYLKQVQAAETKKGPTQIARAAE
                     YTGLIGLVLDAKNAILPGYDPVVKPAPAELTVASPDAPEEIADEGNNNSSLINNSGNQ
                     PSNTGTGGGGGNNNLSLINEPNNQPSSGNGTTTEEAITPEPEPTETPETPEIPETPET
                     SESPESSESPATEPSEPEPEPEPDSAPEPEIEPEPEPEPEPEPEN"
     gene            complement(2170..>2834)
                     /locus_tag="PKK37_03700"
     CDS             complement(2170..>2834)
                     /locus_tag="PKK37_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR81516.1"
                     /translation="MKTAQNNYAFIDAANLFYGGEKSLGWKIDYQKLLRYLKDKYKIS
                     RALYFGGVEIHRFKFDYMDNDAVSLIELEKYSSELIKKDGGKLSEAELILLGRHLQRI
                     RFYLKLDKFGYEMHLKPVKLYDQDDGSTKKKANCDVDMAFWLMKERDNFDRALILSGD
                     GDFLPVLKYLRNHNKEILILSRGPRTAREIRQFAGAHFRDFEYLKYLLKMPKKIQRNG
                     LI"
BASE COUNT          685 a          655 c          592 g          902 t
ORIGIN      
        1 ggggccgttg gtgagggtgc cgtggttgtt gttgccgctg gcgtcgcggg cgacggtgcc
       61 atcgccttcg tcaaagctcc aatagcccac cagcccccgc gtcatcgtgt cctcgggaat
      121 cgccgccccg cccccgaaaa acccattgag cgccagcgtc ccgccggtgg ccgccaacgc
      181 caagacgccg acggcaatca ataatttata attgataaat atttttttgc cgaagattga
      241 taatttataa ttgattcgtt ttttggataa cttatcattg ataattgata aatgattact
      301 gataattgat aatttggcgc caaaaaaacc ggcggcgttg ccgcgttttt tgaatttaaa
      361 cccgaatttt ggaattttga attgcatttt ccccttgccg attatcacca atcattgata
      421 attaataatt aataatttat caatgataat ttatcattta tcactcatca gttatcactt
      481 attatttatc gcttatcaat tattatttat caattatcat ttattattta ttaattatta
      541 tttattaatt atcgattatc accaactcct gcccttccac ttcgcctgct cgcctttgac
      601 gattttgatc accttgttga tttccaccgc cagctcttcc agcatttttt gcatttcttg
      661 gtataaatca tcgctgacca gtgatcgctt gcgcgttttc tccagccaca gcttgcattc
      721 atacagggag ccgcgcgcat aatacagaaa cacgcgatat tcgccgaaaa aataccggcc
      781 gtagccctcg gcgatgttgg cggcaatgga atcggctgcg cgcacgaatt ggtcgcccag
      841 tgttttcttt tcaaaaaaat tccatttgac aatcaaatcc caaacttgat cggaaaaatc
      901 atcggcaagg cggtagattg acagctcttc cagcggtttt gatttttttg actgattgtt
      961 gataattgat aaccgatcat tggcggcttt ctcgctataa tatttccccc agtctatcgt
     1021 gccggcggcg gtgcggtaat tttcatattc gtcgcccata atttataact tataattgat
     1081 aattttatca ttaataattg attatttata aatgataatt tatcatttat cacttatcat
     1141 ttatcaatta tcgcttatca gttttccggc tctggctcgg gttctggttc tggttctggt
     1201 tcaatttccg gttcgggcgc agagtcgggt tcaggttcag gttcaggttc agagggttca
     1261 gttgcagggg attcagaaga ttcaggagat tcagatgttt cgggggtttc agggatttca
     1321 ggagtttcag gggtctccgt gggttcgggt tccggagtaa tcgcctcttc ggtggtggtg
     1381 ccgttgccgg atgacggctg gttattgggc tcatttatca atgataagtt attattcccg
     1441 cctccgccgc cggtgccggt gtttgacggt tgattgcccg aattatttat caatgatgaa
     1501 ttattattcc cttcgtccgc gatttcttcc ggcgcgtcgg gagatgcaac ggttaattcc
     1561 gccggcgcgg gtttgaccac cgggtcatag ccgggcaata tcgcgttctt ggcatccaaa
     1621 accaaaccga tcaaaccggt atattccgcg gcgcgggcga tttgcgtggg cccttttttt
     1681 gtttcggcgg cttgaacttg ttttaaatag ttttgctcgt tttgggcgat ctgcaccagc
     1741 tgttcctgaa catagaggcc gagatctttg ccggccagcg acaatttgtc aatcgccatt
     1801 gacgaccggc gctgggcctc ttgccagatg ttgtcttgga tttccagcgt tcggaaagaa
     1861 taatccacat agtagttgtt ttcgtcttcg gcaacatcat tgattttaat ctcaaaatca
     1921 aaattatcca gatcggcggc cagaattgca gaattgtcaa cgccggattc ggtgataatc
     1981 tcttcgccca ggaccgcttc gcgcgccgcc ggcgatgcgg caaaaatggc cgcgccgccg
     2041 aaaaacgcca gcatcaagcc aaccacgaac ccgttgtggt atttggcaaa atgcgcgatt
     2101 ttattgattg tttcacttga catatttttt taagatgata aaatttaacc actaaacgtg
     2161 aagcggattc tatatgagtc cgtttcgttg tatttttttc ggcattttca gtaaatattt
     2221 gagatattca aaatcccgaa aatgagcgcc ggcaaattgc cggatttctc tggcagttct
     2281 cggcccgcgg gataaaatta gaatttcctt gttgtgattt ctcaaatact ttagaaccgg
     2341 caaaaaatcg ccgtcaccgg acaaaatcaa cgcccggtca aaattatccc tctctttcat
     2401 cagccaaaaa gccatatcca catcgcaatt ggcttttttc tttgttgaac cgtcatcctg
     2461 gtcatataat ttgaccggtt taagatgcat ttcgtatccg aatttatcca atttcagata
     2521 aaaacggatt ctttgcaaat gccggcccaa taatatcaat tccgcttcgc ttaatttccc
     2581 gccgtccttt tttatcaatt cagacgaata tttttccaat tcaatcaagg aaacggcatc
     2641 attgtccata taatcaaatt tgaatcggtg gatctccact ccgccaaaat acagagctct
     2701 ggatattttg tatttgtcct ttaagtaacg caaaagcttt tggtagtcta ttttccagcc
     2761 caaacttttc tctccgccgt aaaaaagatt tgccgcgtca ataaacgcat aattgttttg
     2821 cgccgttttc atat
//
LOCUS       DAOQWE010000112         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_40470_length_2729_cov_2.915108, whole genome shotgun sequence.
ACCESSION   DAOQWE010000112 DAOQWE010000000
VERSION     DAOQWE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40470_length_2729_cov_2.915108"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1254)
                     /locus_tag="PKK37_03705"
     CDS             complement(133..1254)
                     /locus_tag="PKK37_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81517.1"
                     /translation="MKFKRRQTIENNLTPGRALVIYGPRRIGKTTLLKRYLATQTGKK
                     IFYSTGDDIALREVFRSQSLTKIIDFARPYDIIALDEAQYIPFIGQGAKMMIDAFGEK
                     NIILTGSSSLSIAGEIGEPLTGRHFSMLLLPLALAEMDASQFELGVELEKLLIYGAYP
                     EVLNESDLAKKAKILNELVSSYLFKDALALEAVRSPDTLLDVVKCLAFQIGGEVSVNE
                     IAQTAKADNKTVSRYLDLLEKMFVIKKVRGFSCNLRSEISQKAKYYFLDNGIRNAVIS
                     QFNGLASRDDIGRLWENFIFMEMQKQTNIANKLENYYFWRTHSGQEVDIVKESSGKLA
                     AFECKWQNRGQSAPELWRKNYPQSSFDLIGKDNYLDFLL"
     gene            complement(1448..2578)
                     /locus_tag="PKK37_03710"
     CDS             complement(1448..2578)
                     /locus_tag="PKK37_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR81518.1"
                     /translation="MINKIISKFKPKALDNFFAKLGFFKSKAADIAKHYVSGLMNGFK
                     RFNLQALHLATEESSYSYRQLRYFIGDANWDENQLNEKRIQFMENDVRTKTKENGCIS
                     FDDTSVVKSGKHTDGVAKQYAPGAGQIANCKVVVTAHYSDDMKDFPLDAEGYYHGEES
                     KLDLVCSLMDKAIERNLRFKWFNFDSWYCTDQVVKKAVELEKYFVSYLKSNRIVIWKN
                     KRTSVSDLVKIVSAENQKDAVIDLGKIYIKSLGNYRLIVKNTTCFVTNNFVETPVAII
                     AAYSMRWNIDEFYHRAKDDLSFDQFQVRNGLTIMRHWMMVFLAYTFWMHCKLKGVFSK
                     ICQTAIGSLADFAKLMQNLSFVRMAKKSTNVILAELKLNSLN"
BASE COUNT          752 a          632 c          533 g          812 t
ORIGIN      
        1 tataattttg ctccatattg ttttttgcca caacttcaat gtagcaaatc tggccgatat
       61 ttaaagggtc tgttgccaaa ttggcttcag ggcgaaatta acaaaaatta caggggctgg
      121 ccccctgtaa tttcagagca gaaaatcaag atagttgtct ttgccgatca aatcaaatga
      181 tgattgcgga tagtttttac gccacaattc cggcgcggat tgcccgcggt tttgccattt
      241 gcattcaaac gccgcaagct ttccggaaga ttctttcacg atatcaacct cttgtccgct
      301 atgagtgcgc caaaaataat aattttccag cttgttggcg atattggttt gtttttgcat
      361 ttccataaaa ataaaatttt cccatagccg gccgatatca tcgcgcgacg ccaatccgtt
      421 gaattgcgaa atcaccgcgt tgcggatgcc gttatccaaa aagtagtatt tggccttttg
      481 cgatatctca ctgcgcaaat tgcaggaaaa gccgcgcact tttttgatga caaacatttt
      541 ttccagcaaa tccaagtagc gcgacacggt tttgttgtcg gctttggcgg tttgcgcgat
      601 ttcattgacg gaaacttcgc cgccgatttg aaacgccaaa catttgacca catccagcaa
      661 tgtgtcggga gaccgcaccg cttccagcgc cagcgcgtcc ttgaacagat atgaagaaac
      721 cagctcattg agaattttgg cttttttcgc caaatcggat tcgttcaaaa cttcgggata
      781 agccccatag atcagcaatt tttccaactc aacccccagc tcaaattgac tggcgtccat
      841 ttccgccagc gccagcggca aaagaagcat ggaaaaatgc cggccggtga gcggttcgcc
      901 gatttctccg gcgatggaaa gagatgacga ccctgtcaga atgatgtttt tttcgccgaa
      961 cgcgtcaatc atcattttgg cgccctgccc gataaacgga atgtattgcg cttcatccag
     1021 cgcgataatg tcatatggtc gggcaaaatc aatgatttta gtcaaacttt gcgaacgaaa
     1081 aacttcccgc aatgcaatgt catcgccagt ggaataaaaa atcttcttgc cggtttgagt
     1141 cgccaaatat cgtttgagca aagtggtctt gccgatacgc cgcgggccgt aaatcaccag
     1201 cgcccgtccg ggcgtcaaat tattttcaat agtttgccgc cgcttaaatt tcataatttt
     1261 atggatgata atacctcatt tttatacaaa tatatagtat cacaccccct cgctttctgt
     1321 caaatccata ccctccgcct aaccaaaaaa ttacagaggc tcaacccctg caatttttaa
     1381 aacctatgta aaatcaattt atttactctt cttcaatact ttgacaaaat ccgagccaaa
     1441 tcagccctta attgagcgaa tttagcttta attctgccaa aatcacgtta gtcgattttt
     1501 ttgccatccg aacgaagctc aaattttgca tcagtttggc gaaatccgcc aaactgccga
     1561 ttgcagtctg gcaaatcttt gaaaacacgc ctttcagttt gcagtgcatc caaaaggtgt
     1621 aagccagaaa caccatcatc cagtgcctca taatcgttaa gccgtttctg acttgaaatt
     1681 ggtcaaacga caaatcatct ttggcgcggt ggtagaattc atcaatgttc cagcgcattg
     1741 aatatgccgc gattatcgct acgggagttt caacaaaatt attggtgaca aagcaggtcg
     1801 tgtttttgac aatcaaacga taatttccca aacttttaat atagattttt ccaagatcga
     1861 taaccgcatc tttttgattt tcggcagaga caatttttac caaatcgctt accgaagttc
     1921 tcttgttttt ccaaattacg atgcggttgg atttcaaata tgaaacaaaa tatttttcca
     1981 gctccaccgc ttttttgacc acttgatccg tgcagtacca gctatcgaaa ttgaaccatt
     2041 taaagcgcaa attccgctca atcgccttgt ccatcaacga acaaaccaga tcgagttttg
     2101 attcttcgcc gtgataatat ccttccgcgt ccaagggaaa atctttcatg tcatcggaat
     2161 aatgcgcggt aaccacaact ttgcaattgg caatttgtcc ggcgccgggc gcgtattgtt
     2221 tggcaacacc atcggtatgc tttccggact ttaccaccga ggtatcgtca aaagaaatgc
     2281 aaccgttttc cttggtcttg gtgcgtacat cattttccat gaattgaatt cttttttcat
     2341 tcaattgatt ttcatcccaa ttcgcatcac cgatgaaata ccgcaattgg cggtaagaat
     2401 atgaactttc ttcagtggcc aaatgcagag cttggaggtt gaatcttttg aagccgttca
     2461 tcaatcccga aacgtaatgc ttggcgatat ccgcggcctt gcttttaaaa aatcccagtt
     2521 tggcaaaaaa attatccagc gctttgggct tgaattttga aattattttg ttgatcatgg
     2581 aagtaaaatt gggttaaaat ggggttgcaa cgtgttttag cttcatttta ctctcattta
     2641 gcttaaaatt gtagtcgatt ttgtcaaagt attgattatc ggttgatacc aatcccaatt
     2701 ttcagccgta agagttaata gagcaccag
//
LOCUS       DAOQWE010000113         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_40664_length_2721_cov_3.975994, whole genome shotgun sequence.
ACCESSION   DAOQWE010000113 DAOQWE010000000
VERSION     DAOQWE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40664_length_2721_cov_3.975994"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..>2721
                     /locus_tag="PKK37_03715"
     CDS             120..>2721
                     /locus_tag="PKK37_03715"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNR81519.1"
                     /translation="MTKIFESTIEEFVIEVLQNQGWQYLGPEDQELERPNLCEVVLKN
                     RLEKAIDDLNPDISQEAKEQALRAVLNLPSQNLIDNNEAFHRLLTEGVEVEVMGAEGI
                     KGDKVKLVDFVNPGNNEFVVCNQFTVVENNIIKRPDVVLFVNGLPLAVIELKNPVDEN
                     ATVAKAFTQLQNYKNAIPSLFFYNAVLVASDGLDARAGTISADMSRFMAWKSVDGIKE
                     DATTTPQIETLTRGMFKKNVLLDLVLHFIVFEKNKKEDSKTGLISVVATKKIAAYHQY
                     YAVNKAVESTLRATAETQMMVREKPESYGLAGVSLQPKGDHKAGVVWHTQGSGKSLSM
                     VFYTGKTVLKLNNPTVVVMTDRNDLDDQLFDTFAACKQLLRQDPIQAESRDNLKKLLR
                     VAGGGIIFTTIQKFFPDNGAQNFDLLSERKNIIVIADEAHRSQYGFGAKTSEKDGEVF
                     TKYGFAKYLRDALPNASFIGFTGTPIEKEDASTPAVFGNYIDVYDIAQAVEDGATVRI
                     YYESRLAKVHLKPEEAAKLDEEVESITEGEESTATQKAKTKWAQLEAIVGHKDRLRVI
                     AADIVDHFEKRREAFEGKGMIVCMSRRVAVELYEEIIKIRPSWHDSDLKKGAIKVVMT
                     SSSSDPGNWQIHHTTKQDRKAVGERFKDENDNLKLVIVRDMWLTGFDVPCLHTMYVDK
                     PMRGHNLMQAIARVNRVFKDKPGGLIVDYIGIASDLKTALATYTASGGKGAPAFDQSE
                     AIATMLEKFEIVEQMFVNFNYKDYFSADTRQKLTIILEAQEHILGLEDGKNRYTKQVA
                     FLSQAFALSVPSLKAMEIKNEVGFFQAVKARLTKFEPAGSGKSDAEIETAIRQIVDKA
                     VVSEGIVDVFD"
BASE COUNT          805 a          508 c          691 g          717 t
ORIGIN      
        1 gaaagagtat ggcgcaaaag aaagtttcgg aataatggcg cacatgggcg gaattaattt
       61 tgaaaacgat attttaacgg tacccttggg tgtgtttttg ttgatttctt gaaataatca
      121 tgactaaaat ttttgaatcg acaattgagg agtttgttat tgaggttttg caaaatcagg
      181 gttggcaata tttaggaccc gaggatcaag agttagagcg tccaaattta tgcgaagtgg
      241 ttttgaaaaa tcgtttggaa aaggcgattg atgatttgaa tcccgatatt tcccaagaag
      301 ccaaagagca agcattgcgc gcggttttga atttgccgag ccagaatttg attgataaca
      361 acgaagcttt tcatcgttta ctcaccgaag gggtagaggt tgaagtaatg ggcgcggagg
      421 gaatcaaggg cgacaaggta aaactcgttg attttgttaa tccgggaaat aatgagtttg
      481 tggtttgcaa tcaatttaca gtggttgaaa ataacatcat taaacggccc gatgtggttt
      541 tattcgtaaa tggcttgccg ttggccgtga tagaactcaa aaatccggtt gatgagaatg
      601 ccactgtcgc caaggctttt acgcagctcc aaaactataa aaatgccatt ccttcgcttt
      661 tcttttataa cgccgtattg gttgcttccg atggcttgga tgcgcgcgcg ggaacaattt
      721 cggcggatat gtcgcgtttt atggcgtgga aaagcgttga tggtataaaa gaagatgcta
      781 cgaccacgcc gcaaattgaa actttgacca gggggatgtt caagaaaaat gttttgcttg
      841 atttggtttt gcactttatc gtttttgaaa aaaacaagaa agaagattcg aaaaccggtt
      901 tgatttccgt tgttgcgaca aaaaagattg ccgcgtatca ccaatactac gccgttaata
      961 aggcggtaga gtccacattg cgcgccacgg cggaaacgca aatgatggtg cgcgaaaagc
     1021 cggaaagtta tggcttggcc ggcgttagct tacagccgaa aggcgatcat aaggcgggcg
     1081 tggtttggca tacacaagga agcgggaaat cgctttccat ggttttttat accggcaaaa
     1141 cggttttaaa acttaacaat cccacggttg tcgttatgac ggacagaaac gatcttgacg
     1201 accagctttt tgataccttt gccgcttgca aacaattatt gcgtcaggat ccgatccagg
     1261 cggaaagccg cgataattta aaaaaacttt tgcgggttgc cggcggcgga attattttta
     1321 ccaccattca aaaattcttt cccgacaatg gagcccaaaa tttcgattta ttgtctgaaa
     1381 gaaaaaacat catcgtgatc gccgacgaag cgcacagaag ccaatatggt tttggcgcaa
     1441 agaccagcga aaaagacggc gaggttttca ctaaatacgg atttgcgaaa tatttgcgtg
     1501 acgcgctgcc gaacgcttca ttcatcggat ttaccggtac gccgatagag aaagaggacg
     1561 cttcaactcc ggcggttttt ggcaattata ttgatgtcta tgatattgct caagcggttg
     1621 aagatggcgc gactgtgcga atttattacg aatcgcgtct tgctaaggtt catttaaaac
     1681 ccgaagaagc cgccaaactg gacgaagagg tcgaatcaat caccgaggga gaagaatcaa
     1741 ccgcaaccca gaaggcgaaa acaaaatggg ctcaattaga ggcaatcgtt gggcataaag
     1801 acagattgcg cgttattgcc gcggatattg ttgatcattt tgaaaaacgg cgcgaagcgt
     1861 tcgagggaaa aggaatgatt gtttgtatga gccgaagggt tgccgtggaa ttatacgaag
     1921 aaattataaa gatccgcccg tcttggcacg atagcgattt gaaaaaaggc gcgattaaag
     1981 tggtaatgac ttcgtcgtct tccgacccgg gtaactggca aatacatcac accacaaaac
     2041 aggacaggaa agctgttggc gagagattta aagatgaaaa cgacaatctt aaattggtaa
     2101 ttgtgcgcga catgtggctt accggttttg atgtgccttg tttgcatacg atgtatgttg
     2161 ataaaccgat gcgcgggcat aaccttatgc aggccatcgc ccgagtgaat cgggtgttca
     2221 aagataaacc gggaggattg attgttgact atatcggcat tgcgtcggat ttgaaaaccg
     2281 cgcttgcgac ttataccgcc agcggcggaa agggtgctcc cgcttttgat caaagcgaag
     2341 caatcgcgac tatgttggaa aagttcgaaa ttgtggaaca aatgtttgtg aattttaatt
     2401 ataaggatta tttttccgcc gatacccggc aaaaacttac cattattctt gaagcacaag
     2461 aacacattct tggattggaa gacggtaaaa acagatacac aaaacaagtg gcttttttgt
     2521 cgcaggcgtt cgccctgtcc gttccaagct tgaaagcgat ggaaattaaa aatgaagttg
     2581 gattttttca agcggttaag gcacgattga ccaaatttga accggccgga agcggaaaat
     2641 cggatgccga aattgaaacg gcaattcgcc aaattgttga taaggccgtt gtttccgaag
     2701 ggattgttga tgtttttgac g
//
LOCUS       DAOQWE010000114         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_41008_length_2707_cov_6.210784, whole genome shotgun sequence.
ACCESSION   DAOQWE010000114 DAOQWE010000000
VERSION     DAOQWE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41008_length_2707_cov_6.210784"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1786
                     /locus_tag="PKK37_03720"
     CDS             38..1786
                     /locus_tag="PKK37_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81520.1"
                     /translation="MFKIFRKREAPIPQSKLPITKRAKFTSLLFIAVSIFVGSAIIWA
                     ADMYYDIDAQKVVINEVQRVVKSGATALEILGVAKFGDGGLADYLQIDQTGDLNFFGA
                     ADTVSKSDGALTIQTAGQNLNLQTTGSGTLLVSSAGALNFSANATSTWNLGANTLAVS
                     SQNFNVSADGNITAQAGYGLDTIAEGALNLGNNTATSVNIGNTAATSLGMGAGGSLER
                     TINIGTGTGADIINIGTGGGADVITLGGNSGTLAIDTGNWDISATGEMTGISGITTNG
                     GYTQTGSGANTFSGAASFSGAASFTAAGTGLAVTNNATVGGTLGVTGLTTLGGNLTFS
                     GTTARTITGPATGGMTLTVAGGPMGLTTTGSGAITLNSAGQLLLQFGAAGGFTLGDGT
                     TNYFQIAADGTLTLATGAGDEIVLNPAGAAAGVVRLGPGDVILTDGGGAAMRTSGEEI
                     MRGMAPIFGFDMPARTASTGYIAVSRELEDYPFPAAMTGTTRVHKFVIRYADTVNTQT
                     TWRVYNTTDSETTATFTVPASASSNLEKGEVYTTDAITVPDDTDDWRLDVSVADGSGG
                     TIQIYQIFLAAYDRVN"
     gene            1819..2187
                     /locus_tag="PKK37_03725"
     CDS             1819..2187
                     /locus_tag="PKK37_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR81521.1"
                     /translation="MTNQIQNPNVKNKYNLEERTAVFGESIIGFAKTLPKDAIVFPLI
                     SQLVRSSTSVGANYMEADCAESKKDFRHKIGICKKEAKETTHWLRMMAKATPDKKNEC
                     REFWREAHQLVLIFSAILRK"
     gene            2299..>2707
                     /locus_tag="PKK37_03730"
     CDS             2299..>2707
                     /locus_tag="PKK37_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81522.1"
                     /translation="MKKFIPPTKNKITALAFAILPVLITGLLAYGANIYYDLDAAKIV
                     NGENNELTQNLTVAGSVGIGTTSPAGALHVMGQCVTGDTKLRRRRKKSKSQGSNSKDE
                     YEYEDVEIKDIQAGDEILTLDENTGRLTVSRVNA"
BASE COUNT          685 a          696 c          762 g          564 t
ORIGIN      
        1 ttatcagtta tcaatgatta tttattaacc aaaaaatatg ttcaaaattt tcagaaaacg
       61 ggaggcgccg ataccgcagt caaaattgcc gattaccaaa agagcgaaat ttacctctct
      121 gctttttatc gccgtgtcaa tttttgtcgg ttcggcgatt atttgggcgg cggatatgta
      181 ttatgacatt gacgcgcaaa aagtggtgat caacgaggtt caacgcgtgg tcaagtccgg
      241 cgccaccgcg ctggaaattt tgggcgtcgc caaattcggt gatggtggct tggctgatta
      301 tttgcaaatt gatcaaaccg gcgatttgaa tttttttggc gccgccgaca cggtttcaaa
      361 atcggacggc gcgctcacga ttcagaccgc ggggcagaat ttaaatttgc aaaccaccgg
      421 ctcgggaaca ctgctggtgt catcggcggg cgcgttgaat ttctcggcaa acgcgacatc
      481 aacatggaat ttgggcgcca acacgctggc ggtgtcgtcg caaaacttta atgtttccgc
      541 cgacggcaac atcaccgcgc aagccggtta cgggctggac acgattgccg agggcgcgct
      601 gaatttgggc aacaacacgg cgacatcggt gaatatcggc aacaccgcgg cgacatcgct
      661 tggaatgggc gcgggcggga gcttggagcg cacgattaac atcggcactg gcacgggggc
      721 agacatcatc aacatcggca ccggcggcgg cgcggatgta atcacgcttg gcggcaattc
      781 ggggacgctg gcaattgata ccggcaattg ggatatttcg gccaccggcg agatgacggg
      841 aatttccgga atcaccacca acggcggcta cacgcagacc ggtagcggcg ccaacacttt
      901 ctccggcgcc gcctcatttt ccggcgcggc gtcattcacc gcggccggca ccggactggc
      961 ggtaaccaac aacgccacgg tcggcggcac gctgggcgtg accggtttga cgaccctggg
     1021 cggtaatttg acattttcgg gcactactgc gcgcaccatc accgggccgg cgaccggcgg
     1081 catgacgttg acggtggccg gcgggccgat gggcttgacg accaccggtt ccggggcgat
     1141 cacgttaaat tccgcggggc aattgctgtt gcagtttggc gccgccgggg gattcacctt
     1201 gggggacggc acgaccaatt atttccagat tgccgcggac ggcactttga ctctggccac
     1261 gggcgcgggc gatgaaatcg ttttgaatcc cgcgggcgcg gcggcgggag tggtgcggct
     1321 tggtcccggc gacgtgattt tgacggacgg cggcggcgcg gcaatgagaa cttcggggga
     1381 agaaattatg cgcggaatgg cgccgatctt cgggtttgat atgcccgcgc gcaccgcgtc
     1441 aacgggctac atcgccgtgt cgcgggagtt ggaagattat ccgtttcccg cggcgatgac
     1501 gggcaccacg cgggtgcaca aatttgtgat ccgctatgcc gacacggtga acactcagac
     1561 cacctggcgg gtctacaaca ccaccgacag tgagacaacc gcgacattca ccgtacccgc
     1621 gtcggcgtca agcaacttgg aaaaagggga agtctacacc accgacgcaa tcaccgtgcc
     1681 ggatgacacc gatgactggc ggctggacgt gagcgtcgcc gacggctccg gcggcacgat
     1741 acaaatctac cagatatttt tggcggccta cgaccgggtg aactaatcca atgcaaaaat
     1801 ccaaatgtca aaatccaaat gacaaatcaa atccaaaatc caaatgtcaa aaataaatac
     1861 aatttggagg aaaggactgc tgtttttggc gaaagcataa ttggttttgc aaaaactttg
     1921 ccaaaagatg caattgtttt tccgctgatt agccaattgg tgcgttcttc aacaagcgtg
     1981 ggcgcaaact atatggaagc cgattgcgcc gaatcaaaaa aagattttcg ccataaaatc
     2041 gggatttgta aaaaggaggc gaaagaaacg acccattggt tgcgaatgat ggcaaaagca
     2101 acgccggata aaaagaatga atgccgggaa ttttggcggg aggcgcacca attagtgctg
     2161 atcttttccg caattttgcg aaaatgaaac gagagatatt ttaattttga aattgggatt
     2221 tgatttgaca tttggatttt gacatttggg tttgcaaaga ctcatatatc caaataccaa
     2281 accaaaccct aagtccaaat gaaaaaattt atcccgccaa ctaaaaacaa aatcaccgcc
     2341 ttggcgtttg cgatattacc ggtcttgatc accgggcttt tggcctacgg cgccaacatc
     2401 tattatgatc tggacgcggc caaaatcgtc aacggcgaga acaacgaact cacccaaaac
     2461 ctcaccgttg ccggtagtgt gggcatcggc accacctctc ccgccggcgc cctccacgtg
     2521 atggggcagt gcgttaccgg cgacaccaaa ttgcgccgcc ggcgcaaaaa atccaaatcc
     2581 caaggctcaa attccaaaga tgaatacgaa tacgaggatg tggaaattaa agacattcaa
     2641 gcgggggatg aaattttgac gttggacgaa aacaccggcc ggctgacggt gtcgcgcgtc
     2701 aacgcgc
//
LOCUS       DAOQWE010000115         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_42359_length_2649_cov_7.005783, whole genome shotgun sequence.
ACCESSION   DAOQWE010000115 DAOQWE010000000
VERSION     DAOQWE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42359_length_2649_cov_7.005783"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..164
                     /locus_tag="PKK37_03735"
     tRNA            92..164
                     /locus_tag="PKK37_03735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:125..127,aa:Ala,seq:tgc)
     gene            278..754
                     /locus_tag="PKK37_03740"
     CDS             278..754
                     /locus_tag="PKK37_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR81523.1"
                     /translation="MAIIKKNKSDCGGFTLIELLVVIAIIGTLSGMVLVATQGMRESA
                     RIAKARSEVSTIYRAIMMMEARTGKYPVPSNINTAANFRTYLADYLPGIGDDPWGHAY
                     YYDGCPEPCSSCATSCEAGIWNTSVCSGGPNGSISSHNRSPISDDICVYFVGGKSW"
     gene            893..1579
                     /locus_tag="PKK37_03745"
     CDS             893..1579
                     /locus_tag="PKK37_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81524.1"
                     /translation="MTITNEWSAITLQALQNVWEGALLFLPKLAIGLIVFGIGWIIAS
                     GMGKITANILKKINFDSLFSKSGWKAALENAEIRVHPSEFLGAILKWILVIVFLQIFV
                     EILGLSAFAVFLTRVLEWLPNVIVAAAIFVVSIIVADILEKVIRASVKRLEIKYSNLL
                     GAIVRTAIYTFAVMAILLQLGVAETIISTVITGFVGMFALAFGLAFGLGGKDAAAEAI
                     RGLRDKLSEK"
     gene            complement(1671..2576)
                     /locus_tag="PKK37_03750"
     CDS             complement(1671..2576)
                     /locus_tag="PKK37_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HNR81525.1"
                     /translation="MDNILDTIGRTPLVEIKKLNPNPAVKIFAKLEGFNPGGSMKDRV
                     ARALIEDAERSGALKKGKTIIEATNGNTGIGIAMVAAIKGYKTIIVSPENANRERKRI
                     IESFGAQVIEVKPELWRQGALDLVEEMAVKDSSLVVLDKFSAKQNCAVHFNTTGKEIV
                     KQISGQIDYFISCIGTGGAISGIARQLRQNWPQVRVIGIQPKIWGADSSIETGIFPPI
                     LNSNLPCGDHDKVMVDIIVEIGQADAFQMAERIIREEGIFAGASSGAAMFTAAEYAKK
                     MEKGTIVTVFPDRGEHYLSTEMFRN"
BASE COUNT          638 a          614 c          654 g          743 t
ORIGIN      
        1 gagcggtttt cttgtaaatt aatgcctttt tgttattgta tagttgtgtt tgcgtttcta
       61 ttgattcatc gctcgtttca tcgctatttt cgggccttta gctcagttgg cagagcgcct
      121 catttgcaat gaggaggtcg ccggttcaaa tccggcaagg tccactgaaa tcatcagacg
      181 agagacgggg ataatccatt tgacagttga ataaaatact tgccgattat cggaaaggtt
      241 attttaccgt cttttgaagt cttagttttg gggaataatg gcaatcatca aaaaaaacaa
      301 aagcgattgc gggggattta cattaattga attgttagta gtaatcgcga ttatcggcac
      361 gctgtcagga atggttttgg tggccacgca aggtatgagg gaaagcgcaa ggattgccaa
      421 agcccgttcc gaagtcagca cgatatatcg ggcaattatg atgatggagg cgagaaccgg
      481 aaaatatccc gttccgtcaa acataaatac cgcggcgaat tttagaactt atctggcgga
      541 ttatcttccc ggaatcggag atgatccttg gggccatgct tattattacg acggttgtcc
      601 cgaaccttgt tcaagctgtg cgacgtcttg tgaggcggga atatggaaca cctcggtatg
      661 ttccggaggc cccaacggat caatatcgtc ccacaaccgt tctcccatca gcgatgatat
      721 ttgcgtctat tttgtcggcg gcaaatcatg gtaaaaattt ccgtcagcct tgacaaacaa
      781 taaatatcta tattataata taggcataaa accgggcctt ttccgggccc ggcgttgcgc
      841 cgcagtcggg cgcgaggtat aatgtcaaaa aataagttta aaacaaaaaa atatgacaat
      901 caccaatgaa tggtcggcaa tcactctgca ggccctgcaa aatgtctggg aaggcgctct
      961 gctgtttttg cccaaattgg caatcggttt gatcgttttc ggcatcggct ggattatcgc
     1021 ttccggaatg ggcaagatca ccgccaacat tctcaagaag atcaattttg attcgctgtt
     1081 ttcaaaaagc ggttggaagg ccgctctgga aaacgccgaa atcagagtgc atccttccga
     1141 atttttgggt gcgattttaa aatggatttt ggtgatcgtg ttcttgcaga tcttcgtgga
     1201 aattttggga ttgagcgcat ttgcggtgtt cttgacccgc gtgcttgagt ggttgcccaa
     1261 tgtgattgtg gccgcggcaa ttttcgtggt atcaatcatc gtggccgaca ttctggagaa
     1321 ggtcatcaga gcatcggtga aaagattgga gatcaaatac agcaaccttt tgggagcgat
     1381 tgttcgcacg gcgatctaca ctttcgcggt gatggcaatc ctgctccaat tgggcgtggc
     1441 agaaaccatt atcagcaccg tgatcaccgg atttgtggga atgtttgcgc tggcattcgg
     1501 tttggcattc ggcttgggcg gcaaagatgc cgccgcggaa gccattcgcg gattgcgcga
     1561 caaactctcc gaaaaataat ttaaaaacta tccccaaaac ccccgcaccg cgggggtttt
     1621 ggcttgcaag aacaagaaaa agttgcatag gtgtcaccta tgcaactttt ttaatttcga
     1681 aacatttccg tggacaagta gtgttcgccg cgatcgggga agacggtgac aatcgttccc
     1741 ttttccattt ttttggcata ttcggcggca gtaaacattg ccgcgccgga gctggcgccg
     1801 gcaaaaattc cttcttcccg gatgattctt tcggccattt gaaaggcatc ggcttgcccg
     1861 atttccacga taatgtccac catcactttg tcatggtcgc cgcagggcag gtttgaattc
     1921 agaatgggcg ggaatatgcc ggtttcaatg gaagaatccg cgccccaaat cttgggctgg
     1981 atgccgatca ccctcacttg cggccaattt tgccgcagtt ggcgggcaat gcccgaaatc
     2041 gcgccgccgg tgccgatgca ggaaataaaa taatcaattt gcccgctgat ttgcttgaca
     2101 atttccttgc cggtggtgtt gaaatgcacc gcgcaatttt gtttggcaga aaatttatcc
     2161 aaaaccacca atgaagagtc cttgaccgcc atttcctcca ccagatccag cgcgccttgc
     2221 cgccagagtt cgggcttcac ttcaatcact tgcgcgccaa agctctcaat gattcttttt
     2281 ctttcccgat tggcgttttc cggcgacacg atgatggttt tatagccttt gatggccgct
     2341 accatggcaa tgccgatgcc ggtgttacca ttggtcgcct caatgatcgt ttttcccttt
     2401 ttcagcgcgc cggatctttc ggcatcctca atcaacgccc gcgccacgcg atctttcatt
     2461 gatccgcccg ggttaaaacc ttccagcttg gcaaagattt taacggcggg attgggattt
     2521 aattttttga tttccaccag cggcgttctg ccgatggtgt caagaatgtt gtccatagaa
     2581 tttgttaagg cttattatta gacctactgc gtaaatattt cctactgcaa tttccatatt
     2641 tcggctacg
//
LOCUS       DAOQWE010000116         2643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_42515_length_2643_cov_4.163060, whole genome shotgun sequence.
ACCESSION   DAOQWE010000116 DAOQWE010000000
VERSION     DAOQWE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42515_length_2643_cov_4.163060"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PKK37_03755"
     CDS             complement(<1..115)
                     /locus_tag="PKK37_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR81526.1"
                     /translation="MINDKSGITGFTLIELLVVIAIIGTLSGMVLVAMTGAR"
     gene            complement(147..389)
                     /locus_tag="PKK37_03760"
     CDS             complement(147..389)
                     /locus_tag="PKK37_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81527.1"
                     /translation="METDQNKDIDLSDYSAKTPPASGIVAPPDFGKQERKKKIQIIIV
                     IAGFILTAAFWGYYFYGQNAKNQPSNYDISGEMIPE"
     gene            complement(401..847)
                     /locus_tag="PKK37_03765"
     CDS             complement(401..847)
                     /locus_tag="PKK37_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81528.1"
                     /translation="MSCLVVIAIIGTLSGMVLVAMTGVREKARDIIRKQHLDAIQAAL
                     ELYYQEYEHYPPEGQTYDTSIGAGNSLPNPPQGSWSSGSDLQDLVTTGIIKTVPIDPI
                     NNATYYYYYEPDAINQNGCKVNSCRWVLRCRLEATGAYYYLKSAEQ"
     gene            complement(1098..1934)
                     /locus_tag="PKK37_03770"
     CDS             complement(1098..1934)
                     /locus_tag="PKK37_03770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81529.1"
                     /translation="MKTDITNKIFNEDALLGIDKIADSSVDLIVADPPYCLGKDYGND
                     SDKLSSSDYLEWMFRWFDLVYGKLKENGSLYIFLSWQYTPEIFSYFKKKMRMVNEIIW
                     DRHVPSMGGSTRKYSSVYDSIGFFVKNDDYYFDLDSIRVPYDEATKKARTRSIFVGKK
                     WLEMGYNPKDLWSYSRIHKQDPERRNHPTQKPLFIIERIIKASCPIGGLVLDPFMGSG
                     TTAVASVRNNRNFVGFEINENYYKDSLDRVEIEKQKGKQLGLLLEASSASFDKEKTII
                     KA"
     gene            complement(1931..2527)
                     /locus_tag="PKK37_03775"
     CDS             complement(1931..2527)
                     /locus_tag="PKK37_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81530.1"
                     /translation="MEETDKILVKMDFLRTIFTKIITLLPKFDIADKTVLPYGLKPHT
                     RSVSWIVEQVIVQQAKFRKDELGLDEVSFDMPDTCLHDCVILKGGQKYFVNVKIHNID
                     GKENKNDISAVEKLYFQYSANPDYDLVFACVGVSFKENSIFFDQNYLEIFSAQFLPIY
                     VNPRNDKIQAKYNHKMEIRSRKDFLKLLKKNSKSIILK"
     gene            complement(2532..>2643)
                     /locus_tag="PKK37_03780"
     CDS             complement(2532..>2643)
                     /locus_tag="PKK37_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004806255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNR81531.1"
                     /translation="DSLENGDVTVRDRDTMAQIRIKIADLENYLRDNLGN"
BASE COUNT          743 a          628 c          438 g          834 t
ORIGIN      
        1 cccgcgcccc cgtcatcgcc accagcacca tccccgacag cgtgccgatg atggcaatca
       61 ccaccagcag ttcaatcaga gtgaagccgg ttatgcctga tttatcattt atcattgata
      121 attgataatt atcatttatc tcaaaatcat tccgggatca tttctccgga aatatcataa
      181 ttgctcggtt ggttcttggc gttttggcca tagaaataat atccccagaa cgccgcggtc
      241 aatataaacc ccgcgataac gatgattatc tggatttttt tctttctctc ctgcttgccg
      301 aaatccggcg gcgcaacaat gccggaagcc ggcggagttt tggccgaata atcactgagg
      361 tcaatgtctt tgttttgatc cgtctccata ttttttaaaa ttattgctct gcacttttaa
      421 gatagtaata agcccccgta gcttctaaac ggcaacgaag aacccaccta cagctattta
      481 ctttgcaacc gttttgatta atagcatcag gctcatagta gtaatagtag gtagcgttat
      541 tgatagggtc tataggaact gtttttataa ttcctgtagt aaccaaatct tggagatcgc
      601 tacccgaaga ccaactgcct tgagggggat tagggagact atttccggct ccaatactag
      661 tatcgtaagt ctgtccttcc ggcgggtaat gctcatattc ttggtagtat aattccaaag
      721 cggcttgaat ggcatccaaa tgctgttttc ggataatatc tcgggctttt tcccgtaccc
      781 ccgtcattgc caccagcacc atccccgaca gcgtgccgat gatggcgatc accaccaaac
      841 agctcaatca gagtgaagcc gcccttgtcc gattttaggt tggcaaaaaa ctcaaacatc
      901 cgccttcgcc ccgcggtcac gcgaaatccg aacctgccga atccgatttc ccgcggcaca
      961 aattaaaatt ttctcctgcc cgcaaaggtc tgaccctcac attgacacca ctattcccga
     1021 caacgtgttt gtgttttgca catacatcca ttggcatagc ccgtttccca atgtgatttt
     1081 ctgatacatc ttttttatta cgccttaatt atcgtttttt ctttatcaaa acttgcgcta
     1141 gacgcttcaa gtaagagccc aagttgtttt cctttttgtt tttcaatttc aacccgatcc
     1201 aaagaatcct tataataatt ttcattaatc tcaaaaccga caaaatttct gttatttcta
     1261 acacttgcca ccgcagtcgt tccactcccc ataaatggat ccagcaccaa tccgccaatg
     1321 ggacagctgg ccttgattat tctttcaatt ataaaaagtg gtttttgcgt cggatgattg
     1381 cgcctttccg gatcttgctt gtgtatccgg ctataactcc ataagtcctt tggattatat
     1441 cccatttcaa gccatttttt tcccacaaat atgctgcgag ttctcgcttt ttttgttgcc
     1501 tcgtcatagg gaactctgat tgaatccaaa tcaaaataat aatcgtcgtt tttcacaaaa
     1561 aacccaatac tgtcataaac cgaagaatat tttctcgtgg aaccgcccat actcgggaca
     1621 tggcgatccc aaataatttc atttaccatc cgcatttttt tcttgaaata agaaaaaatt
     1681 tccggcgtgt attgccagct taaaaaaata tacaagctac cattttcttt taatttgcca
     1741 taaaccaaat caaaccagcg aaacatccat tccaaataat ccgatgaaga caacttatcg
     1801 gaatcgttgc catagtcctt tcccaagcaa tatggcggat cagcgacaat taaatcgaca
     1861 gagctgtcgg cgattttatc tattcccaaa agagcatcct cattaaaaat tttattagta
     1921 atatcggttt tcattttaga ataatgcttt ttgagttttt ctttaataat ttcaagaaat
     1981 cttttcggct ccttatttcc attttgtgat tatacttcgc ctgtatttta tcattgcgag
     2041 gattaacata aatcggcaaa aattgcgcgg aaaaaatttc caaataattt tgatcaaaga
     2101 atattgaatt ttctttaaaa ctgacgccga cgcacgcaaa caccaaatca taatcaggat
     2161 ttgccgaata ttgaaaatat aacttttcta ccgcggaaat atcattttta ttttccttgc
     2221 cgtcaatatt atgaatttta acattaacaa aatatttttg cccgcctttt aatatcacgc
     2281 aatcatgcag acaagtatcc ggcatatcaa agctgacttc gtccagaccc aattcatctt
     2341 ttctaaactt cgcctgttga acaatcactt gttctacaat ccaactcact gaacgggtgt
     2401 gcggttttaa cccatacggc agaacggttt tatcggcaat atcaaatttg ggaagcaatg
     2461 taattatttt tgtgaaaatc gtccgcaaaa aatccatttt gactaaaatt ttatctgtct
     2521 cttccataaa attaatttcc caaattatca cgcaaataat tttccaaatc agcgattttt
     2581 attctaattt gcgccatggt gtcgcggtcg cgcacggtga catcgccgtt ttccagcgaa
     2641 tca
//
LOCUS       DAOQWE010000117         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_43690_length_2597_cov_2.454367, whole genome shotgun sequence.
ACCESSION   DAOQWE010000117 DAOQWE010000000
VERSION     DAOQWE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43690_length_2597_cov_2.454367"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKK37_03785"
     CDS             complement(<1..315)
                     /locus_tag="PKK37_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81532.1"
                     /translation="MQTLLSALRKDHLGREEFLKEFERVLAFVKQLKNTNKQVISGAY
                     ERVNLLALRLKENNEQDIEKAKREMAGLIEDYLSKVETATNQKMREVDIKIKELKNGI
                     DGK"
     gene            complement(460..2211)
                     /locus_tag="PKK37_03790"
     CDS             complement(460..2211)
                     /locus_tag="PKK37_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81533.1"
                     /translation="MDNIIDQMPTPVKRKVNAVDWLIRTPEKVLRKIGLGKEMDNLRK
                     GYDAYLKELPKNIDKITEWTKQVPTKENNQNIFLYLDGQDVKLNPTELKVAGEIKTWL
                     SEWADRLKLPKDNRITHYITHLFDEQLIKKEFDEDLAKIISQKIPGSVYDPFLIKRLG
                     AKGYKVDTWAALDAYVKRGTRKVNLDPALEKIDEIAPSLEQSQWDYLKEYIDRVNLRP
                     TKIDTSIDNTIKSVVGYKFGQRPTAMISRVIRQAGYRGGLWFNVSSTLRNLSQGINTY
                     AVLGEKYTALGYAKLFSKANRQEIIDQGVLNAGFIDDRILSATQKLWQKADNVGFAMF
                     QAVEGINRGSAYLGAKAKALSMGKNEAQAIEYGKKISRETQFTFGSIDTPLAFSNDIV
                     KTLMQFQTYTTKQVEFLSELAKDKNFGALARYTAAGLLYVATIGQAFGMDWKELIPAW
                     RLPAPPAGKFLATTAGAILNAPDKYGNQRDFPKKLKDIAATAWGIVPGGSQAKKTIQG
                     YQSIQEGGSYDAAGRLQFKQDQSTAGQLQSLLFGKYAGQNAQNYFDKKGTKTKESGLP
                     ALPKLPKLPTLPKLPQL"
     gene            2261..2509
                     /locus_tag="PKK37_03795"
     CDS             2261..2509
                     /locus_tag="PKK37_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81534.1"
                     /translation="MDIASAGVFSVLFVLGAFASMGVASPRGVGWVVNSCGFFIAGVV
                     CFNDKSFLVGNLGNAGNPPLVGGFLLALFQMSMNWDFG"
BASE COUNT          617 a          562 c          515 g          903 t
ORIGIN      
        1 cttgccgtca atgccgtttt tcagttcttt gatttttatg tccacctcgc gcattttttg
       61 atttgtcgct gtttcaactt tggataaata atcttcaatc aatccagcca tttcccgctt
      121 tgctttttca atatcttgct cgttgttctc ttttaggcgc aacgccaaaa gattgacgcg
      181 ctcatacgcg ccggatatta cttgcttgtt ggtatttttt aattgtttga caaacgccaa
      241 aacacgttca aattctttta aaaattcttc gcgtccgaga tggtctttgc gtaaggcaga
      301 taataatgtt tgcaaatttt gtaaatcttc ttgcatataa aaaaaacaac gcccgcccaa
      361 ctcaatggga cagacgctgt cgttttatgg ataagcgttc tgacgcttct attttaccac
      421 tttgtttgga aatcagtcaa gtgaggtttg taggtaaaat tataattgag gtaatttagg
      481 aagtgtgggg agtttaggta attttgggag agcgggcaaa ccgctttctt ttgtttttgt
      541 tcctttcttg tcaaaataat tttgtgcgtt ctgtccggcg tatttgccaa acaaaagcga
      601 ctgcaactgc cctgcggttg attggtcttg cttgaattgc aagcgaccgg cggcgtcata
      661 cgaaccgccc tcttgaattg attggtagcc ttgtattgtt tttttggctt gcgaaccgcc
      721 cggaactatt ccccaagcag tcgcggcaat atcttttaat ttctttggaa aatctctttg
      781 gttgccgtat ttatccggcg cgtttaaaat cgctcctgcg gttgttgcta agaactttcc
      841 cgccggtggc gcgggcaatc tccaagcagg tatcaattct ttccaatcca taccaaacgc
      901 ttgcccgatt gtggcgacat acaataatcc agcggcggta tatcttgcca gcgcgccaaa
      961 gtttttgtct ttggcaagct cgcttaaaaa ctcaacctgt ttagtggtgt atgtctgaaa
     1021 ctgcatcaat gtttttacaa tatcgtttga aaaagcaagc ggggtatcaa tagagccaaa
     1081 agtaaattgc gtttctcggg aaatcttttt gccatattca atcgcttgtg cttcgttctt
     1141 tcccatactc aatgcttttg cttttgcgcc aagatatgcc gaacctctgt ttattccttc
     1201 aactgcttga aacatcgcaa aaccaacatt atcggctttt tgccataact tttgagtggc
     1261 agataaaatc cggtcatcaa taaatccagc gttcaacact ccttggtcaa taatctcttg
     1321 gcggttggct tttgaaaata atttggcata tcccaaagcg gtgtatttct cgcccaaaac
     1381 cgcataggta tttattccct gtgataagtt gcgcaatgtt gaagaaacat tgaaccaaag
     1441 ccctcccctg tatccggctt gacgtattac gcgcgaaatc atcgcggttg gtctttgccc
     1501 gaatttataa ccgacaacag attttattgt gttatcaata cttgtatcaa tttttgtcgg
     1561 tcgtaaatta actcggtcaa tgtattcttt taagtaatcc cattgtgatt gttccaaaga
     1621 cggcgcaatt tcatcaatct tttctagcgc tgggtcaaga ttaactttcc tcgtgcctcg
     1681 cttgacatac gcgtccaacg ccgcccaagt atccaccttg tatcctttcg cgccaagacg
     1741 cttgattaag aacgggtcat agacgcttcc aggtatcttt tgggaaataa tctttgccaa
     1801 atcctcgtca aattcttttt tgataagttg ttcgtcaaac aagtgagtaa tgtagtgagt
     1861 aatgcgatta tctttcggaa gtttcaacct gtccgcccac tcgctcaacc aagttttaat
     1921 ttcaccggca acttttaatt cggtaggatt aagttttaca tcttgtccgt caagatataa
     1981 aaagatattt tgattgtttt cttttgtcgg gacttgtttt gtccattcag ttattttatc
     2041 aatgtttttc ggcaattctt tcaaatacgc gtcatatcct ttccgcaaat tatccatctc
     2101 tttaccaagc ccgattttgc gcaaaacctt ttccggtgtt ctaataagcc aatcaaccgc
     2161 attaaccttt ctcttgacag gagtaggcat ttggtctata atgttatcca atgaaacatc
     2221 aaccttttgc cctgcgtaat ctaatatctg cgactccgaa ttggatattg cttccgctgg
     2281 cgtgtttagt gtgctttttg ttctgggcgc ctttgcttct atgggcgtgg cttcaccacg
     2341 aggggtgggc tgggtcgtca attcttgcgg tttttttatt gctggcgtgg tttgttttaa
     2401 tgataaatct tttttagtag gcaatttggg caacgccggc aaccctcctt tggtgggggg
     2461 ttttttgttg gctttattcc agatgtcgat gaattgggat tttggataat aaccaaattc
     2521 gttaatatca tctcctgcaa agagaatatc ttttgctatt actttaaacg agtgaacttt
     2581 ttctgttcct tcaattg
//
LOCUS       DAOQWE010000118         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_44819_length_2554_cov_6.111645, whole genome shotgun sequence.
ACCESSION   DAOQWE010000118 DAOQWE010000000
VERSION     DAOQWE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44819_length_2554_cov_6.111645"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..1057)
                     /locus_tag="PKK37_03800"
     CDS             complement(215..1057)
                     /locus_tag="PKK37_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR81535.1"
                     /translation="MKFTGERMIPEFNKEDLIYAEHMARYFFAGQFVAAKDVLDVASG
                     SGYGTRYLLNKGAAKVVGIDNSREAIKYSQEKYQTSGIEFILADAAKLPFENDIFDIV
                     VSFETIEHLDDQEKFLQEIKRVLRKDGLLIISTPNVLVFPKGNTFHKKELTPFEFISL
                     LSKNFRHHSINYQHSILSSYVFGEKNLACDHIPARFENVKLSPIPPDKNMYLVAIASD
                     GCLPEPISDLVVLHNDDEIASLNRQIEYFRNTGFGKIAGFIHRFRKRMPIIKNLPGYF
                     SKHK"
     gene            complement(1058..1462)
                     /locus_tag="PKK37_03805"
     CDS             complement(1058..1462)
                     /locus_tag="PKK37_03805"
                     /inference="COORDINATES: protein motif:HMM:NF017345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HNR81536.1"
                     /translation="MAQSLKYNLQNLAIHRDARGWLVEMLKQNEVGQKIAQIYVASLC
                     PGAIRGNHWHSKRIEWFFVIGGDVQVHLQDTKTKKTKIIDIRSDNPSRISIFPPVAHA
                     VVNKGENVAYLISAQNNIYDPGDSDTFEYKIL"
     gene            complement(1447..2436)
                     /locus_tag="PKK37_03810"
     CDS             complement(1447..2436)
                     /locus_tag="PKK37_03810"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNR81537.1"
                     /translation="MKKILITGGAGFVGHHCVEHFLKNTDWKVVVLDSLNYAGNLNRI
                     TDSEYFDAKRIGFIWHDLCSPISKTTRDMIGDLDYMIHFAAESHVDKSLEDSVPFVKS
                     NVLGTANLMEYLKNFQPHCRTVVFSTDEVFGPAPQGVYYKEDDRFKPSNPYSAAKAGE
                     EMIAYSFAHAFGLPVFVVRSMNIIGERQHPEKFLPKVIRSIIDDKKITLHGLGEADLS
                     SRCWIHARNVADGIVFLLDKGQSGEFYHIVGEERSVLEIANWVSQAVRGKNLDESRIE
                     WLDYHKARPGHDKRYALSGQKMAAMGWEPPVNLEESVKKTVDWTLKNPVWLNL"
BASE COUNT          713 a          546 c          578 g          717 t
ORIGIN      
        1 gccagaaatg atctttttgg aaattggaaa ttgaaacatt gaaatttttt aataaataga
       61 cctactgcgt aaatatttcc tactgcaatt tccatatttc ggctgcgccg aattcgtcaa
      121 tcgtcggcat atttaagcca atatgcctcc tcattcctca ttcggctcgc gcgaaatctg
      181 aaaatttcgt cgcgtaaata tttacgcagt aggtctattt atgttttgaa aaatatccgg
      241 gcagattctt gataatcggc attctttttc tgaaacgatg aataaagccg gcaatttttc
      301 caaatcccgt attgcgaaaa tattcaattt ggcggtttaa agacgcgatc tcgtcatcat
      361 tatgtaaaac aaccaaatcg gaaattggtt cgggcagaca gccgtcactg gcaatggcaa
      421 ccaaatacat atttttgtcg ggaggaatgg gcgaaagctt aacgttctca aaccgggcag
      481 gtatgtgatc gcaagcaaga tttttttcgc caaatacata actgctcaaa atgctgtgtt
      541 gataattgat tgaatggtga cggaaatttt ttgacaataa agatataaac tcaaaaggag
      601 tcagttcttt tttatggaag gtgttgcctt tggggaaaac cagcacattg ggggtggaga
      661 taatcaacaa gccgtctttt ctaagaactc ttttgatctc ttgcaaaaat ttttcctggt
      721 cgtccaaatg ttcaatggtt tcaaaagaga caacaatgtc aaaaatatca ttttcaaagg
      781 gaagtttggc ggcatcggca aggataaatt caatgccgga tgtttgatat ttttcttgtg
      841 aatatttgat agcttcgcgg gaattgtcaa tgccgacgac ttttgccgct cctttgttta
      901 aaaggtagcg cgtgccgtag ccggaaccgc tggcaacgtc caaaacgtct ttggcggcca
      961 caaattggcc cgcgaaaaaa tagcgggcca tatgctcggc ataaatcaaa tcctctttat
     1021 taaattcggg gatcatgcgt tcgccggtaa atttcattta caagatttta tactcaaagg
     1081 tatcggaatc gccgggatca tagatgttgt tttgggcgct tatcaaatag gcaacatttt
     1141 cacccttatt tacgaccgcg tgggcaacgg gcggaaaaat cgaaatccgc gaaggattgt
     1201 cggaccggat gtcaatgatt tttgtttttt tggtttttgt gtcctgaaga tggacctgga
     1261 catcgccgcc gatgacaaaa aaccattcaa tgcgttttga gtgccagtga ttgccgcgaa
     1321 tggcgccggg acaaagcgaa gcaacatata tttgcgcgat tttttggccg acttcatttt
     1381 gcttgagcat ctcaaccagc cagccgcgcg cgtcgcgatg aatcgccaaa ttttgaagat
     1441 tgtattttaa agattgagcc atacgggatt tttcaaagtc caatcaacgg tttttttaac
     1501 ggattcttcc agattgaccg gcggctccca acccatcgcg gccatttttt gcccggagag
     1561 cgcatatctt ttgtcatggc cgggacgggc tttatggtaa tcaagccatt caatccggga
     1621 ttcatccaaa ttcttgcccc ggaccgcctg ggaaacccag ttggcgattt ccaaaaccga
     1681 acgctcttcg ccgacaatgt gataaaactc gccgctttgt cctttgtcaa gcaaaaaaac
     1741 gattccgtcg gcgacattgc gggcgtgaat ccagcaacgc gacgaaaggt cggcttcgcc
     1801 caagccgtga agcgttattt ttttatcatc aataattgag cggatgactt tgggcaaaaa
     1861 tttttccgga tgctggcgtt cgccgatgat attcattgaa cggaccacaa aaaccggcaa
     1921 accaaaagca tgggcaaaag aataagcgat catttcctct ccggctttgg cggccgaata
     1981 aggattggag ggcttgaaac gatcgtcttc tttataataa accccctgcg gcgcggggcc
     2041 gaaaacctcg tcggtggaaa aaacgacggt gcggcaatgc ggttgaaaat ttttcaaata
     2101 ttccattaaa ttggcggtgc ccaaaacatt ggacttaaca aagggaacgc tgtcttccag
     2161 gcttttatca acgtgcgatt cggcggcaaa atgaatcatg tagtccagat cgccgatcat
     2221 atcgcgggtg gttttggaga tcggcgaaca caggtcatgc cagataaacc caatccgttt
     2281 agcatcaaaa tattcgctgt cggtgatgcg attcaagttc ccggcgtaat tcagggagtc
     2341 caaaacaaca accttccaat cggtattctt gagaaaatgt tcaacgcagt ggtggccgac
     2401 aaaacccgcg ccgccggtga ttaaaatttt tttcatattt tttgattagg cattgtaaag
     2461 aaataagttc ctaagattgg ttgtcagatt tgccccagat ttcgcttttc cgaccaagca
     2521 atatttgaag tgtagcaata gctacattga aaat
//
LOCUS       DAOQWE010000119         2528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_45580_length_2528_cov_3.935301, whole genome shotgun sequence.
ACCESSION   DAOQWE010000119 DAOQWE010000000
VERSION     DAOQWE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45580_length_2528_cov_3.935301"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1104)
                     /gene="wecB"
                     /locus_tag="PKK37_03815"
     CDS             complement(19..1104)
                     /gene="wecB"
                     /locus_tag="PKK37_03815"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNR81538.1"
                     /translation="MTKKIKIMVVVGARPNFIKIAPLFWEFRKRPEFAPILIHTGQHY
                     DYEMSRIFFKDLDIPAPDINLGIGSGTHAWQTGQSMIELEKIMAEKKPGAVAVIGDVN
                     ATLAGSLAAAKLHIPVAHIEAGLRSFNKSMPEEINRVLTDHISDFLFCPTRNAIANLK
                     REGIIKGVFNTGDIMLDVLNAKLKKCHPAILEKLGIEPKKYLLLTVHRAGNVDNAENF
                     KKIIDAVSRIGEKVVFPTHPRTMAQLNNLDLKNFFAPQGNVIGIDPVGYMEMLSLEKN
                     AKMILTDSGGVQKEAFWLKIPCVTLRNETEWVETVAIGANVLVGTDKEKIIRLAHHFV
                     FPKTEKNYYGIGVAARKIADKLLDVLQ"
     gene            complement(1097..2212)
                     /locus_tag="PKK37_03820"
     CDS             complement(1097..2212)
                     /locus_tag="PKK37_03820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR81539.1"
                     /translation="MEFFDLKKQYQDLKSEIDAAVLDVLEDGVFIGGPAVEKFEKEVA
                     DFLGVKYAVSLNSGTDALYLSLRALGIGAGDEVITTAFTFFATAEVVAAAGAKPVFVD
                     IDAKTFNIDADQIEAAITEKTKAIIPVHLFGQMADMAPIKKIAQKHNLKIIEDCAQAI
                     GATQIIDGKEAKAGNIGDCGCFSFFPTKNLGAYGDGGMATTNNDEIAVKIRMLKVHGS
                     SPENKYKNLEIGVNSRLDAIQAAILSVKIKYLDQWNDQRAAIAKRYGEAFEGVGDIVA
                     PFAAPNNRHIYHQYTIRTAKRDQLVQFLKNAEIPAMIYFPFPMHLEPALKYLGYKMGD
                     FPEAERAAAEVLSLPIYPELSSKEQAVIIAATKKFYD"
     gene            complement(2220..>2528)
                     /locus_tag="PKK37_03825"
     CDS             complement(2220..>2528)
                     /locus_tag="PKK37_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNR81540.1"
                     /translation="KYPKKDFSQYGQWLSTLIKEGATIGANATIVCGTVVGKWAFIAA
                     GAVVKGEVPDYALMAGVPAKQIGWMCECASKLVFKNGMAACKICGRKYIKSGNVINES
                     "
BASE COUNT          642 a          639 c          509 g          738 t
ORIGIN      
        1 taaaaaaagg tttaatggtc attgaagaac atcaagcaat ttatcggcaa ttttccgggc
       61 cgcaactccg atcccgtaat agtttttttc tgtttttgga aaaacgaagt gatgtgccag
      121 acggattatc ttttctttgt ctgtgccgac cagaacgttt gcgccaatgg caacagtttc
      181 aacccattcg gtttcattgc gcaaagtaac gcacggtatt ttcagccaaa acgcttcctt
      241 ctgaaccccg ccactgtcag tcaaaatcat tttagcgttc ttctccaaag acagcatttc
      301 catatatccc accggatcaa tcccgatgac attcccttgg ggagcaaaaa aatttttcaa
      361 gtcaagatta tttaattgcg ccatcgtgcg cggatgtgtc ggaaaaacaa ccttttcccc
      421 gatgcgagag accgcgtcaa tgattttttt aaaattttcc gcgttatcaa catttcccgc
      481 gcgatgaacc gtcagcaata aatatttttt tggttcaatt cccaattttt ccaagattgc
      541 cggatggcat tttttcaatt ttgcatttaa aacgtccagc ataatatcgc cggtgttaaa
      601 cacgccctta ataattcctt cccgctttaa attggcaatc gcgttccgcg tcggacaaaa
      661 caaaaaatcg gaaatatgat ccgtcaacac ccggttaatt tcttcgggca ttgatttgtt
      721 gaaactcctc aaccctgcct caatatgcgc caccggtata tgcaactttg ccgcggccag
      781 agaccccgca agcgttgcat taacgtcgcc aatcaccgca accgcgccgg gttttttttc
      841 cgccatgatt ttttccaatt cgatcatgga ttggccggtt tgccaagcat gggttccgga
      901 accgattccc aaattgatat cgggcgcggg tatatccaga tctttgaaaa atattcgcga
      961 catttcataa tcataatgtt gccccgtgtg tatcaaaatt ggtgcgaatt ccggccgttt
     1021 tctaaattcc caaaacagcg gcgcaatttt gataaaattg ggccttgctc cgaccacaac
     1081 catgattttt atttttttag tcataaaatt ttttggttgc ggcgataatt accgcttgtt
     1141 ctttgctgga caattccggg tagatcggca ggctcaaaac ttcggcggcg gcgcgttcgg
     1201 cttcgggaaa atcgcccatc ttatagccca aatacttcaa tgcgggctcc aagtgcatcg
     1261 gaaaaggaaa gtatatcatt gcgggaattt ccgcattttt caaaaattgc accagttgat
     1321 cgcgttttgc ggttcttatt gtgtattgat gatagatgtg gcgattgttc ggcgcggcaa
     1381 acggcgcaac aatgtcgcca acgccttcaa atgcctcgcc atagcgtttg gcgattgccg
     1441 cgcgctggtc attccattga tcaagatatt tgatcttaac gctcagaatg gcggcctgaa
     1501 tcgcgtccaa acgagaattg acgccaattt ccaaattttt gtatttattt tcgggagatg
     1561 atccatgaac ttttaacatt ctgattttta ccgcaatttc gtcgttattg gtcgtcgcca
     1621 ttcccccgtc accgtaagca cccaagtttt tggtggggaa aaaagaaaaa cagccgcaat
     1681 cgccaatatt gccggccttg gcttccttgc catcaataat ttgtgtcgcg ccgatggctt
     1741 gcgcgcaatc ttcaataatt ttcagattat gtttttgcgc gatcttttta attggcgcca
     1801 tatctgccat ttggccgaaa agatgcaccg gtatgatcgc ttttgtcttt tccgtaatcg
     1861 ccgcttcaat ttggtcggcg tcaatgttaa aagttttcgc atcaatatca acgaatacgg
     1921 gtttggcgcc ggcggccgcg actacttcgg ccgtggcaaa aaaagtgaat gccgtggtaa
     1981 tcacctcatc tcccgcgcca atccccaacg cccttagcga caaataaagc gcatcagtgc
     2041 cggaattcag gctgacggca tattttaccc ccaaaaaatc ggcaacctct ttttcaaatt
     2101 tttccaccgc cgggccgccg ataaacactc cgtcttcaag cacatccaaa accgccgcgt
     2161 caatctcgga ttttagatcc tgatattgct ttttcaaatc aaaaaattcc atatttgcat
     2221 tacgattcat ttataacatt gcccgatttt atatatttcc gaccacagat tttgcatgcc
     2281 gccataccgt tcttaaaaac aagtttgctg gcgcattcgc acatccagcc aatttgcttg
     2341 gcgggaaccc cggccatcaa cgcataatcg ggaacttccc ccttgacaac cgctccggcg
     2401 gcaataaatg cccatttgcc aacgacagtc ccacaaacga ttgtggcatt ggcgccgata
     2461 gtcgctccct ctttgattaa agttgaaagc cactggccat attgcgaaaa atcttttttg
     2521 ggatattt
//
LOCUS       DAOQWE010000120         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_47933_length_2448_cov_3.829085, whole genome shotgun sequence.
ACCESSION   DAOQWE010000120 DAOQWE010000000
VERSION     DAOQWE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47933_length_2448_cov_3.829085"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1865
                     /locus_tag="PKK37_03830"
     CDS             318..1865
                     /locus_tag="PKK37_03830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNR81541.1"
                     /translation="MAKNTNGKTEPFEKTLFKAADKLRKNMDAAEYKHIVLGLIFLKY
                     ISDSFEELFAKIKEGKGDFSGANPEDPDEYRAENVFFVPRVARWSYLHSRAKLPSIGK
                     DVDDAMEAIEKENQTLKGILPKVYARPNLDKAALGGLIDLIGNIALGDAAAKSKDLLG
                     RVYEYFLGEFANAEGKNAGQFYTPKSIVRLMVEMIEPYKGRVFDPACGSGGMFIMSEK
                     FVQEHRGNVDDISIYGQESNQTTYRLCRMNLAIRGIDGSQVKWNTEGSFLNDLHKDLK
                     ADFVLANPPFNDSDWSGQLLQKDARWQYGMPPASNANYAWLSHMIYHLSPKGVMACVL
                     ANGSLSSQTNNEGEIRKSLVENDLVDCIVALPKQLFYNTGIPACIWFISRKRTGNGDR
                     KRTGEILFIDASEIGFMADRTHREFTEKDVAKVSETYHEWRKQNGKYEDIKGFCKSAN
                     LEEIAKHNFVLTPGRYIELKDEEDDGIPFNEKITGLTRKLAEQMGEEKKLDEEIKKQL
                     KNIGYDL"
     gene            1855..2109
                     /locus_tag="PKK37_03835"
     CDS             1855..2109
                     /locus_tag="PKK37_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81542.1"
                     /translation="MICNLLINLNFWSALSGLIGTILIFFFGLPPKVDPDGHIHLICE
                     QEDEKEKIKGKKYKAISYIGLVLIGLSFLFQLLNTILTIN"
     gene            2112..>2448
                     /locus_tag="PKK37_03840"
     CDS             2112..>2448
                     /locus_tag="PKK37_03840"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNR81543.1"
                     /translation="MADQTTTIIKGWKMTPLGEMGVAITGKTPSKDNPSDWGDFLDFI
                     TPTDIVSDSKYLNNVARKLSEDGANRFKKMIIPPKSVVVTCIGSDMGKVVMNRNEALT
                     NQQINSIKIN"
BASE COUNT          797 a          439 c          544 g          668 t
ORIGIN      
        1 gattgatatt actccaattt cctctgacgg ccatttcgca ttcaagaaaa taaaagtgtt
       61 atatttatgc tatatttaaa cataacattt tcgcgaatca ggaaactacg cccgcacaac
      121 gcattttagt ggtagcgacc gagggaagtc ccgcgtattc ccgcgggagt gggggtaaag
      181 agaaagtatt ttgcataaaa aatatataat attgaagcca gcaggcttcg tgattttttt
      241 aaaaaaattg aattgaaaaa aagaagctaa aagagttatt cttatcaaga aagaggctta
      301 tttcataaat agtttttatg gctaaaaata ctaacggaaa aaccgaacca tttgaaaaaa
      361 cactttttaa ggcggcggac aagcttcgta aaaatatgga tgccgccgaa tataagcaca
      421 tcgttcttgg gcttatattc ttgaaatata tctctgattc ctttgaggaa ctttttgcga
      481 aaataaaaga aggtaagggt gatttttccg gcgcaaatcc cgaagatccc gacgagtatc
      541 gggccgaaaa tgttttcttt gttccgcgag tcgcccgctg gtcatattta cactcgcgcg
      601 ccaaacttcc gtcaattggt aaagatgttg atgacgcaat ggaagcgatt gagaaggaaa
      661 atcaaacact caagggcatt ctgcccaaag tgtacgcgcg tccaaattta gataaagcgg
      721 cacttggcgg cttaatcgat ttaattggaa atatcgcgct gggcgatgcc gccgccaaaa
      781 gcaaggattt gctcgggcgc gtttatgaat attttctcgg tgaatttgct aatgcggaag
      841 gaaagaacgc cggccaattt tatacgccca aatccattgt tcgcttgatg gtggaaatga
      901 tcgagccata taaaggcaga gtttttgatc cggcttgcgg ttctggcggg atgtttataa
      961 tgagcgaaaa attcgtccag gagcatagag gtaatgttga cgatatttca atttatggac
     1021 aagaaagcaa ccaaacaact tatcgtttgt gccgaatgaa tttggcaatc cgcggaattg
     1081 atggctcgca ggtgaaatgg aataccgaag gatcattttt aaatgattta cataaagatc
     1141 tcaaagcaga tttcgtttta gccaatccac cgttcaacga tagcgactgg tcgggtcagc
     1201 ttttgcaaaa agacgcgcgc tggcaatatg gcatgccgcc cgcgtcaaac gcgaattatg
     1261 cgtggctttc gcatatgatt taccacttat cgcccaaagg ggtgatggcc tgcgttttgg
     1321 cgaacgggtc gctttccagc caaaccaaca acgaaggaga aatcagaaaa tcgttagtgg
     1381 aaaatgattt ggtggattgt atcgtcgcgc ttcccaaaca gcttttttat aataccggta
     1441 ttccggcgtg catttggttt atttcgcgca aacgaacggg caacggcgac cgcaaacgca
     1501 ccggtgagat tttgtttatt gatgcctccg aaatcggttt tatggcggat cgaacccatc
     1561 gcgaattcac cgaaaaagat gtcgcaaaag tttccgaaac ttaccacgaa tggcggaaac
     1621 agaacgggaa atatgaagac ataaaaggct tttgtaaatc agcgaatttg gaagaaattg
     1681 ccaaacataa tttcgtgctt actcccggac gatatattga acttaaagac gaagaggatg
     1741 acggtattcc atttaatgaa aaaattacgg gattaactag aaaacttgcc gaacaaatgg
     1801 gcgaagaaaa gaaactggac gaagaaataa aaaaacaatt gaaaaatatt ggttatgatt
     1861 tgtaatttat tgataaatct gaatttttgg agcgcgttat cgggcttgat aggaactatt
     1921 ttaattttct ttttcggttt gccgcccaag gttgatccag acggtcacat tcatttaatt
     1981 tgcgagcaag aagatgaaaa agaaaaaata aagggtaaaa aatataaagc gataagctat
     2041 attggcttag ttttaattgg tttgagtttt ttgtttcagc ttttaaatac gattttaact
     2101 attaattgat aatggcggat caaacaacaa caattataaa aggctggaaa atgacgccgc
     2161 tgggcgagat gggcgtcgct attactggta aaaccccctc aaaagataac cctagcgatt
     2221 ggggtgattt cttagatttc attactccga cagatattgt ttccgactca aaatatttaa
     2281 ataatgttgc gagaaaatta tcggaagatg gggccaatag attcaaaaaa atgattatcc
     2341 caccaaaatc agtcgtagta acttgtattg gttccgatat gggaaaagta gtaatgaaca
     2401 ggaatgaagc acttacaaat cagcagataa actctatcaa aattaatt
//
LOCUS       DAOQWE010000121         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_49647_length_2394_cov_4.148354, whole genome shotgun sequence.
ACCESSION   DAOQWE010000121 DAOQWE010000000
VERSION     DAOQWE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49647_length_2394_cov_4.148354"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..2121)
                     /locus_tag="PKK37_03845"
     CDS             complement(253..2121)
                     /locus_tag="PKK37_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81544.1"
                     /translation="MNLKSKILAAGLSAIIAGGGIFAANMNTAQAQPAESTDVAQLEA
                     QVLQLQQQIAQISALIVQLKSQETCGNGICRFGETTASCPADCNTTSIKVSANSLLSN
                     YVYAGDKKVLIYSLNILANGSDMDVQRVTLQFSGAPYKYLSNIYLYNGNTLLTSSPLN
                     NTTVSTVSNFDHEITLSNFSNKFVIPKGTAGRLDIKVDVISPLAGVGAIAKMYVITAP
                     HGIRAVDQSNVNYYGSESSETMGFMMIISNKPACGNNVCEAETGESTINCPADCSGTA
                     PGLLPNASKCTLNSECASGSCSYGTCCTAAQCGWGDDGAGGPRMCVDSKDEKTNQWLQ
                     GICINGTWETVLNGIGCNNFACLSGTCINNKCTCTTADCGKRTCYGEGEGEIGLTAGT
                     AISCCSGLTAQPRTTTSPNGTQAVTEGFVCRKPVVASCKKEGELFGYLEGECCEGLLK
                     VYPDGAKTYSAGESQNFQVTCKKPASYITNAPAKCVAVNIKALANNKYVAAENGGASP
                     LIANHDTAGPWELFYILPAADGSINIKAGFNGKYVTAENGGASPLIANRDTAGPWEKF
                     VLAPISNTNEVAIMASINNRYVTAENGGAQALVANRDEIGPWEKFTITKVSDCQIQ"
BASE COUNT          531 a          599 c          565 g          699 t
ORIGIN      
        1 ttaattttta cttataatat tttttgttaa caaacgattg cgtcgaccat tatcggtgga
       61 aatcgcaaaa acaccgcttg cgtcattccg tttttataat accataaatt gccgcaacaa
      121 gcacaatatc cgctccgtaa actcaccggg aaagggtcta tccctcgtca atacgaggaa
      181 tggacccttt aaattcattc tttgcgcctc ctttggccat actgcctttg gttattcatt
      241 cgccagaata gtttattgga tttggcagtc ggacactttg gtgatcgtga atttttccca
      301 cgggccgatt tcgtcgcgat tggcgaccag tgcctgcgcg ccgccatttt cggcggtgac
      361 gtagcgatta tttatggagg ccatgatcgc aacttcattg gtgttggaaa tcggggcgag
      421 gacgaatttt tcccacgggc cggcggtatc gcggttggcg atcagcgggc tggcgccgcc
      481 gttctccgcg gtcacatatt tgccgttaaa cccggccttt atgttgatac tgccatcggc
      541 cgccggtaaa atataaaaca attcccacgg accggcggta tcgtggttgg cgatcagcgg
      601 gctggcgccg ccgttctctg cggccacgta tttgttgttt gcaagagctt taatgttgac
      661 cgcgacacat ttagccgggg cgttcgtgat ataacttgcc ggcttcttgc aagtgacttg
      721 aaagttttgg ctttctccgg ccgaataggt tttggcgccg tcgggataaa ctttcagcaa
      781 gccctcgcaa cattcaccct ccaaataacc gaacaattct ccttcttttt tgcaacttgc
      841 aacaacgggt ttccggcaaa caaaaccttc cgtgaccgct tgcgtgccgt tcggagaggt
      901 tgttgttctt ggttgcgcgg tcaacccgga gcaacaagat attgccgtcc ctgcggtcaa
      961 tccgatctcg ccttcccctt cgccgtagca agtccttttg ccgcaatccg cggtggtgca
     1021 ggtacatttg ttgttaatgc aagtcccgct taggcacgca aagttattgc aaccaatgcc
     1081 gtttaaaacg gtttcccatg ttccatttat gcaaattcct tgcaaccatt gatttgtttt
     1141 ttcgtctttg ctgtcaacac acatccgcgg tccgcccgcg ccgtcatcgc cccagccgca
     1201 ctgagcggcg gtgcaacaag tgccgtagga gcaacttccg gaagcgcatt ccgaattcaa
     1261 ggtgcatttt gaggcgtttg gcaataaccc tggggctgta ccggagcaat ccgcggggca
     1321 attaattgtc gattcgccgg tttcagcttc acacacatta ttgccgcaag ctggtttgtt
     1381 gctgataatc atcataaacc ccatagtctc ggaagattcg ctaccataat aattcacatt
     1441 tgattggtca actgcgcgta tgccgtgggg agctgtgatc acatacattt tggcaatcgc
     1501 tccaacccct gcaagcggag aaattacatc cacttttata tccaaccggc cggctgtacc
     1561 tttaggaatc acgaatttgt tcgaaaaatt gcttaaggta atttcatgat cgaagtttga
     1621 taccgttgaa accgtggtgt tatttaatgg tgaggatgtc aacagagtgt ttccattata
     1681 cagatagatg ttgctaagat acttgtatgg cgcgccgctg aattgcagag ttacgcgctg
     1741 aacatccata tccgagccgt tggcaaggat attcaaactg taaatcaaca ctttcttatc
     1801 tccggcatac acataattgg acagcagtga atttgcgcta actttgatgg atgtcgtatt
     1861 gcaatccgcg ggacagctgg ccgtggtttc gccgaagcgg cagatgccgt tgccgcaggt
     1921 ttcttgggat ttgagttgca cgatcaaagc gcttatttgc gcgatttgtt gctgtagttg
     1981 cagaacttgc gcttccaatt gcgcgacatc ggtagattcc gcgggttgcg cttgcgcggt
     2041 gttcatattc gccgcgaaga ttccgccgcc cgcgataatc gccgacagtc cggcggccag
     2101 gattttgctt ttgaggttca tattttagat aaattattaa aatttttatt gctacttaca
     2161 atgttactta cattgatacc tacgcattgc ccgattgttg catcggtaac gcacaaggtg
     2221 ttttactttc gacctttttt aaattgcgac tgcaatttca ttgtaaaatt atttcatttt
     2281 ttatgccagc aaggtttctt cccgcgaaac ttcttccacg ttgtcggcca cgattttggt
     2341 ttcgccgtcg cgcagatcaa tgcggccata gacgtaaacc accttgctct cgca
//
LOCUS       DAOQWE010000122         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_49748_length_2390_cov_8.531049, whole genome shotgun sequence.
ACCESSION   DAOQWE010000122 DAOQWE010000000
VERSION     DAOQWE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49748_length_2390_cov_8.531049"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="PKK37_03850"
     CDS             <1..1850
                     /locus_tag="PKK37_03850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNR81545.1"
                     /translation="ASETRCAGEIDHLHFKTAEQQTQGVANKIIEIAKNNPEAEFGNF
                     AILARANESAALFARACQRSGIPARFAALKGLYSKPAVLDVISYLQFLNNYHDDLALW
                     RILNFPFLMIPGGDVAKISQHSYKKSCSIYEALFNQEMRSQLTASGRAGAEKLAALLE
                     SHGKIARQKNVSEITLAFLEDSGYLEWLARRDAREEIDFIGQFFARIKDFEAANLDAG
                     LKNFIDQITMEIESGEEGKLNFDPIRMPDAVQIMTAHAAKGLEFDYVFIVDLVDRKFP
                     TIERGDEIEIPAALEKDAPAANNAHLEEERRLFYVAMTRAKKGLYFASAGDYGGARPK
                     KISRFLHEMGYNGDVCPEEVKMVRVAGNGRRQPRGNLPGHFSFTQLATFEKCPLQYKF
                     AHVLRVPSRGKSFFSYGKTMHNTLYEFLRETGGKKGNFAALAEVYKRNWIDEWYDGKD
                     EKETYYRQGLASLKNFWRDFSARRPQIARIGGELALERDFNLKINGYAITGKIDRIDE
                     SEKGVEIIDYKTGTAKDRLRPEDKMQLMIYQIAAKEVFDLDSEKLTYWYLNEARPLSF
                     SPGGPEIVEQRDKIAGIIEKIVKSDFRAKPGWACAWCEYKSICDFAKKR"
     gene            1891..2139
                     /locus_tag="PKK37_03855"
     CDS             1891..2139
                     /locus_tag="PKK37_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81546.1"
                     /translation="MNTFVKFVFTILGGVLIGLGLGIMAHKNSLLSAGQFEAAMILSL
                     IAGGFFLALGLPGKRIADSEESSQNAAARESDRQYPSA"
BASE COUNT          614 a          559 c          650 g          567 t
ORIGIN      
        1 tggcaagcga aacgcgatgc gcgggagaga ttgaccatct tcatttcaaa accgccgaac
       61 agcaaacgca gggagtcgcc aacaaaatca ttgaaattgc caaaaacaat cccgaagcgg
      121 agttcggcaa ttttgcgatt ttggcgcgtg ccaacgaaag tgccgcgctt ttcgcgcggg
      181 cgtgccaaag atcgggaatt ccggcgcgct tcgccgctct caaaggactc tattccaagc
      241 cggcggtgtt ggacgtgatt tcctatttgc aatttttaaa caattatcat gatgatttgg
      301 ccttgtggcg cattctcaat tttccgtttt tgatgattcc gggcggagac gtcgccaaaa
      361 tcagccagca cagctataaa aaatcatgct caatttacga agcgttgttt aatcaggaaa
      421 tgagatcgca attgaccgca tccgggcggg cgggcgcgga aaagctggcg gcactgctgg
      481 aatcgcacgg caaaatcgcc cggcaaaaaa atgtttccga aatcacgctg gcgtttttgg
      541 aggattcggg ctatctggag tggctggcgc ggcgggacgc gcgcgaagag attgatttta
      601 tcggccaatt tttcgcccgt atcaaagatt ttgaagccgc caaccttgac gcggggctga
      661 aaaattttat tgatcagata acgatggaaa ttgaatcggg cgaggagggt aaattgaatt
      721 ttgatcccat caggatgccc gacgcggtgc aaataatgac cgcgcacgcc gccaaagggc
      781 tggaatttga ttatgtgttt atcgtggatt tggtggatcg caaatttccc accattgagc
      841 gcggcgatga aattgaaatt cccgcggcgc tggaaaaaga cgccccggcg gcaaacaacg
      901 cccatttgga agaagagcgg cggctttttt acgtggcgat gactcgggcg aaaaagggac
      961 tctatttcgc ctcggcgggc gattatggcg gggcgcgccc aaaaaaaatc tcgcgatttc
     1021 tgcatgaaat gggctataac ggagatgttt gtcccgaaga agtgaaaatg gtgcgtgtgg
     1081 cgggcaacgg ccggcgccag ccgcgcggca atttgccggg gcatttttcc ttcactcaat
     1141 tggcgacgtt tgaaaaatgt cctctgcaat acaaatttgc ccatgttttg cgggtgccgt
     1201 cgcgcggcaa gtcatttttt tcctatggca aaacaatgca caacacgctc tatgaatttt
     1261 tgcgggaaac cggcggtaaa aaaggaaatt tcgccgcgct cgcggaagtt tataaaagaa
     1321 attggattga cgagtggtat gacggcaaag atgaaaaaga aacctattat cggcagggat
     1381 tggcgtcatt gaagaatttt tggcgcgatt tttccgcgcg gcgtccccaa atcgcccgca
     1441 tcggcggcga actggcgctg gaacgggatt tcaacctaaa gatcaacggc tatgccatta
     1501 ccggcaaaat tgaccgcatt gacgagagtg aaaaaggagt ggaaatcatt gactataaaa
     1561 ccggcaccgc caaagaccgc ttgcgtccgg aggacaaaat gcaattgatg atctatcaaa
     1621 tcgccgccaa ggaagttttt gatttggatt ccgaaaaact cacctattgg tatctaaacg
     1681 aagcccgccc cttgtcgttt tcgcccggcg ggccggaaat cgtggaacag cgcgacaaga
     1741 ttgccggcat cattgaaaaa atcgtgaaga gcgattttcg cgccaaaccc ggatgggcgt
     1801 gcgcgtggtg cgaatacaaa agtatttgcg actttgccaa aaaacgctga tgcagttttc
     1861 aatttttggt tttcaattta caatgaattt atgaatactt ttgttaaatt tgttttcacg
     1921 attttgggcg gcgtgctgat cggtttggga ttgggaataa tggcgcataa aaacagcctg
     1981 ttgtccgccg gccagtttga agcggcgatg attttgagtt tgattgccgg cggatttttt
     2041 ttggcgctgg gactgcccgg caagagaatc gccgacagcg aggaatcttc tcaaaacgcc
     2101 gcggcgcggg aaagcgaccg ccaatatccg tccgcgtgaa aaccgcctcg cctcgcggtg
     2161 ctctctggca aaaaaggggt ctatccctcg ttactgcgag ggttagaccc ctttaatttc
     2221 gcgcttaaaa ttcttttgcc agggtctaac cgctcgcgag tatccctcgg attacctcgg
     2281 gacaagcgcg agggatagac cctcaaaaga caatccccgg tgagtggttc gggctaggta
     2341 actgctcaca attttgagca tcaatcttaa aaccgcctat tgcccgcata
//
LOCUS       DAOQWE010000123         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_50701_length_2361_cov_10.986990, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000123 DAOQWE010000000
VERSION     DAOQWE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50701_length_2361_cov_10.986990"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..994)
                     /locus_tag="PKK37_03860"
     CDS             complement(422..994)
                     /locus_tag="PKK37_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81547.1"
                     /translation="MSIRLKLSGFGRNLLVFILGIVYGLSLTPSFGGVEAQEETAPES
                     ADIRELVVYGDAAEFKFCGKISRAVFIEPKTPTDICSKAEAASASKAAARRMKVVLTA
                     YSSTVEQTDSTPFITANGTYVRDGIVANNGLPFGTEIRIPELFGEKVFSVQDRMHWRK
                     GGYHFDIWFPTYEQAKNFGVKYAYVEVLEK"
     gene            complement(1252..>2361)
                     /locus_tag="PKK37_03865"
     CDS             complement(1252..>2361)
                     /locus_tag="PKK37_03865"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNR81548.1"
                     /translation="TAMMIATILSRAGFKTAVSSSIEFRIGDRVWKNRSRMTMPGRFA
                     LQKFLRRAADEDCRYAVVEVSSEGIAQYRHRFIRFDAAVLTNLTPEHIESHGGFENYR
                     AAKGKFFAACRNIHVINGDDPNCEFFAAFPAACRYVYGICHSFSGRISAKKITAAKVD
                     FSLGGSSFESEGLAFKLCLPGEFNVYNALAAIAVVRGQGVPPETCRAALESIATIPGR
                     MEKVFAGDFEVLIDYAVTPDSLEKAYQTVRRGLAGKLICVLGACGGGRDRWKRPVMGH
                     LAQKYCDRVFVTSEDPYDENPERIIQEIAAAASNACRITDRKEAIHRSLKAAERGDRV
                     IITGKGCEDSMCLAGGHKIPWSDKDIVLEYLEKSR"
BASE COUNT          603 a          658 c          535 g          565 t
ORIGIN      
        1 gcggaaaggc cgcttgcgcg gccggcgcgg ataatatact taaaaatgcc gcggcacaaa
       61 ccgccgccaa cgaacgcgca aaaacatttt tttctgtttt catattttta aatttatttt
      121 taatttttca aaatgcgaac gcgccaaaaa caaaaccggc aatcaaaaag ccggcaatcc
      181 ttatggcgat attttaattg aaactgatcg caaacggcaa actttatttt ccggcggttt
      241 ttctcctccc atagagaaac aaagtatttt tccggcgcca acgcgcgccg gatgttttaa
      301 ttatatcacc ccttgatttt cccgcaatcc cgccccgccc acgcaaaaaa tcacgatcac
      361 aattcaaaca aaaaaaccgc ccttgattaa acggggcggt ttttttgttt gatattaaat
      421 tttacttttc cagaacttcc acgtaggcgt atttaacgcc gaaattcttg gcttgttcgt
      481 aggtgggaaa ccagatgtca aagtggtatc cccctttgcg ccagtgcatc cggtcctgca
      541 ccgaaaacac tttttcgccg aacagttccg gaatgcggat ctcggtgccg aacggcaggc
      601 cattgttggc cacaatgcca tcgcggacat aagtgccatt ggcagtgata aacggggtgc
      661 tgtcggtctg ctccacggtg ctggaatagg cggtgagaac cactttcatc cgccgcgccg
      721 ccgccttgga agcgcttgcg gcctcggcct tggaacaaat gtccgtcggc gttttgggtt
      781 caataaaaac cgcgcgggaa atcttgccgc aaaacttgaa ttcggcggca tcgccgtaaa
      841 ccaccagctc tcggatatcg gcgctttccg gcgccgtttc ctcctgcgcc tcaactccgc
      901 cgaaagatgg cgttagagac aagccataaa cgatccccag aatgaaaacg agcaaatttc
      961 ggccaaaacc ggacaacttc aagcgaatac tcatatggaa tttgatcgga catagatgcc
     1021 tttctatacc gcagttaggg tttttcgctc gctaaacacc caaatccctt tttgagtgcc
     1081 aaacccgcct tattcataat gacgccctct attctattcc aaaagtaaat tcctgtcaag
     1141 ggtcaaacaa gattaaaatg ccaataatta ttgacatttt taatttgaca aaaaagtaaa
     1201 attgacgtaa aaaacgcgct ctgatggcgc gtttttggct atcgtcgtct tttagcgcga
     1261 tttttccaga tattccaaaa caatgtcttt gtcgctccaa ggaattttgt gcccgccggc
     1321 cagacacatt gaatcttcgc accctttgcc ggtgataatc acccggtcgc ccctctccgc
     1381 agccttcaat gaccgatgaa ttgcctcctt gcgatccgtg atccggcagg cgttgctggc
     1441 ggcggcggcg atttcttgga taatccgttc tggattttcg tcataagggt cctcgctagt
     1501 gacaaaaacc cggtcgcaat atttttgcgc caaatgtccc atcaccggcc gtttccaccg
     1561 atccctcccc ccgccgcacg ctcccaacac gcaaatcaat ttacccgcca acccccgacg
     1621 aacggtttga taggcctttt caagcgagtc gggcgtaacg gcataatcaa tcagaacttc
     1681 aaaatcgccg gcgaaaacct tctccatccg gccgggaatg gtggcaatac tttccaatgc
     1741 cgcacggcag gtttccggcg gcactccctg cccgcggaca acggcaatcg cggccaaggc
     1801 gttataaaca ttaaattcgc cgggcaagca caatttaaag gccagcccct ccgattcaaa
     1861 cgacgacccg ccaagagaaa aatccacttt ggcggcggta atttttttcg cgctaatccg
     1921 gccggaaaaa ctgtggcaaa ttccgtaaac ataccggcaa gcggccggaa atgcggcgaa
     1981 aaattcgcag ttgggatcat cgccgttgat aacgtggata ttacggcaag cggcgaaaaa
     2041 ttttcctttg gcggcacggt aattttcaaa tccgccgtgc gattcaatgt gctcgggcgt
     2101 gaggttggtc agcacggccg catcaaaacg gataaagcgg tggcgatatt gcgcaatccc
     2161 ctcggaactc acttccacca ccgcgtaacg gcagtcttca tcggcggcgc ggcgcaaaaa
     2221 tttttgaaga gcgaatcttc cgggcatcgt catccgggag cggtttttcc aaacgcggtc
     2281 cccgattctg aattctattg acgaagaaac cgcagtcttg aaccccgcgc gcgacaaaat
     2341 cgtagcgatc atcatcgccg t
//
LOCUS       DAOQWE010000124         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_51004_length_2352_cov_6.740966, whole genome shotgun sequence.
ACCESSION   DAOQWE010000124 DAOQWE010000000
VERSION     DAOQWE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51004_length_2352_cov_6.740966"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKK37_03870"
     CDS             <1..711
                     /locus_tag="PKK37_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81549.1"
                     /translation="FISLEIGVLQDNSCLNLDPHNISLTGEITDIDHQETINEENEHN
                     PYSRKKQAAQYASFLGNLIFLADGFNLDKTKVQETFNIKLLSQSKSYEFFDLKRTNEI
                     IDLLEKSKVVVGLKSSKGYDIEENGSEIFSESIKQWQDYKDKLLILEETKKEIELLVD
                     KFQNEIELTSEEKAEETLRGLTKTELINLSKYFPEFINEIVELIKNNSQIERRKMIDL
                     LRKNYEKRIYQKIILLDL"
     gene            716..1834
                     /gene="dnaX"
                     /locus_tag="PKK37_03875"
     CDS             716..1834
                     /gene="dnaX"
                     /locus_tag="PKK37_03875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNR81550.1"
                     /translation="MENLVLYRKYRPQTFADIIGQEHVTKTIQNEIKSGHAAHAYLFS
                     GPRGCGKTTTARVIAKAVNCLNRAANSGEPCNRCANCVEIQSGRAIDLIEIDAASHRG
                     VDDIRELREGIKFAPAKLKYKVFIIDECHQLSKDAANALLKTLEEPPAHAIFVLATTE
                     LHKILPTIMSRCQRFDFRKLSLPELVGRLQKLADNEKIKVEKAALELIALSAGGSVRD
                     AESLLGQILTLAAQKKSAIAAGDIREFLGMTETKSAAELTRFIAEKNAAAAVSHLNEI
                     IEKGYDVAEYGKSLLNYLRHALVLAIVGPKDAKNDNIMIAGLTDEEFEVLRTQTLAMG
                     EEKIKNIIDIFLEAQGKIKYSPIPQLPLEIALVESCSR"
     gene            2209..2282
                     /locus_tag="PKK37_03880"
     tRNA            2209..2282
                     /locus_tag="PKK37_03880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2241..2243,aa:Gln,seq:ttg)
BASE COUNT          727 a          521 c          529 g          575 t
ORIGIN      
        1 ttcatatctt tggaaattgg agtcttgcag gataattctt gtttgaattt ggatccgcac
       61 aatatatctt tgaccggaga aatcaccgac attgaccacc aagaaacgat taatgaagaa
      121 aacgagcata atccatattc caggaaaaaa caagccgcgc aatacgcttc atttctggga
      181 aatttaattt ttctcgccga tggattcaat cttgataaaa caaaagtcca agaaacattc
      241 aatataaaat tattgagcca gtcaaaatct tacgaatttt ttgatttaaa aagaaccaat
      301 gaaattattg atttgttgga aaaatcaaaa gtcgttgttg ggcttaaatc atccaaaggc
      361 tatgatattg aagaaaatgg aagtgaaatt tttagcgagt caatcaagca atggcaagat
      421 tacaaggata aattattaat attggaagaa acaaaaaaag agattgaact attggtagat
      481 aagtttcaga atgaaataga attaacttcg gaagaaaaag cggaagaaac attacgagga
      541 ttgactaaaa cggaattaat caatttgtca aaatattttc ctgaatttat taacgaaatt
      601 gtggaattaa taaaaaataa ttcgcaaatc gaacgccgga aaatgattga tttattgaga
      661 aaaaattatg agaagcgaat atatcaaaaa attattttat tagatttata aaattatgga
      721 aaatttagtt ttatatcgca aataccggcc gcagacgttt gccgacatca tcggccagga
      781 gcacgtcacc aaaaccattc aaaacgagat taaaagcggg catgccgcgc acgcctattt
      841 gttttccggt ccgcgcggtt gcggcaaaac caccaccgcg cgcgtaattg ccaaagcggt
      901 caattgcctg aatcgcgcgg caaacagcgg cgagccttgc aaccggtgtg ccaattgcgt
      961 ggaaatccaa agcgggcggg caatagactt gattgaaatt gacgcggcgt cgcaccgcgg
     1021 cgttgacgat atccgcgaac tgcgcgaggg catcaaattc gcgccggcaa aactcaaata
     1081 taaagtgttt attattgatg aatgccacca gctttccaaa gatgcggcca acgccctgct
     1141 caaaaccttg gaagaaccgc cggcacacgc gattttcgtt ttggccacca ccgaattgca
     1201 caaaattctg cccacgatta tgtcgcgttg ccagcggttt gattttcgca aattatccct
     1261 gcccgaactg gtcgggcgcc tgcaaaaact ggccgacaac gaaaaaatca aggttgagaa
     1321 agccgcgctg gaattgatcg cgctttccgc cggcggctcg gtgcgcgacg ccgaaagcct
     1381 gttgggacag attttgacgc tggcggcgca aaaaaaatcc gccatcgccg cgggcgacat
     1441 ccgcgaattt ttgggaatga ccgaaaccaa atccgccgcg gagctcacgc ggtttatcgc
     1501 cgaaaaaaac gccgccgccg cggtgagcca cttaaacgaa atcattgaaa aaggctatga
     1561 tgtcgccgaa tacggcaaaa gcttgctgaa ttatctgcgc cacgcgctcg ttctggcgat
     1621 cgtcggcccc aaagacgcga aaaacgacaa cataatgatt gccggcctga ccgacgaaga
     1681 atttgaagtc ctgcggacgc aaaccctggc gatgggcgaa gaaaaaatca aaaacatcat
     1741 tgatattttt ctggaagcgc aaggcaagat caaatattct cccatcccgc aactgccctt
     1801 ggaaatcgcc ctcgttgaat cctgctcccg ataatcttgc aatgcaccga aaaaatactg
     1861 tttaaaaaaa atcaggggtt gaacccctga tttttagttt gcaccccaaa aagcatttcg
     1921 ccgttaataa actacgccgg ggcagagccc cgacgtattc cggcgctccg cttcgctacg
     1981 cgatcggaaa agtttgtttc cccgccattc atccccgggg caaagccccg aggtattctg
     2041 gcgggtaaat aaacattcca acattctcaa gaatgttgga atgtccgcta tccgggaaaa
     2101 cgtcaatgag attgatcgtt aaagttcgga agccaaactc ctgatttttt gttgttggtt
     2161 tattgatttt tattgttttt gcgctataat aaacccgttt tgtgaaattg ccggatcgtc
     2221 taatggtagg acaacgccct ttggaggcgt ctatcttggt tcgaatccag gtccggcagc
     2281 cagccgcagt aaaaatgatt aagctcgtcc atttagggcg agcttttcga tgtacttgcg
     2341 gctggtggtg ag
//
LOCUS       DAOQWE010000125         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_51377_length_2341_cov_3.033246, whole genome shotgun sequence.
ACCESSION   DAOQWE010000125 DAOQWE010000000
VERSION     DAOQWE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51377_length_2341_cov_3.033246"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PKK37_03885"
     CDS             complement(<1..938)
                     /locus_tag="PKK37_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81551.1"
                     /translation="MATPAQQYEILGATSKVVSETQTLKIGEGKLQKIITTATTAGTL
                     KVIDGTEASTVATGKLTSGGACAPAVYATQKITSSGACAPADYAKQTLTSSGACAPAA
                     HAKQTLTSNGTNVTDGKTVTINTTTYRFKKTPEQAYDVRIGSTAADTLANLKAAINLS
                     GTMGVEYYVGTAAHTTVIATTLTATTLVVVARTIGTAANSYNTTDNDASLSWGAATMA
                     GGVATTAATITINTTTYTAVKELTESFGLTAVANQVLWKTSEAIFLDNLKKAINGTGT
                     AGSEYSTGTTAHTTVIATTNTDTTQVIVARTIGTAA"
     gene            complement(941..>2341)
                     /locus_tag="PKK37_03890"
     CDS             complement(941..>2341)
                     /locus_tag="PKK37_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81552.1"
                     /translation="MVVPWNRIYCDELDFDGNPKIEIIELTPGQLKQRQGYDQEMVEA
                     LIAAQQSRTDADGRQKDQKNNYIKLYEIHGNLPLSFLTGEEKDQNKYTQQMHVISFQE
                     KKEKGEYDDFTLYKGKEKKDVYMLTWLIPSEDGSISLWGSVKNLFEAQWMTNHTQKSV
                     KDYLDLASKMIWQTSDGNLQGQNALQTIEHGDFIIHQANQPLTLVPTNPQNTIVFENY
                     GRQWEELGNKINGISESMLGSNPPSGTAWRQTEALLTEGHSLFDLMAENKGLAIEKML
                     REFIIPHLKKQFDDKDEIVADLDDYGVDRIDEMFISSQAVKRANEKVVQMVLDGETDI
                     DQQAILGQEKQALQEMLGAMGGKRFYKPDDIDQKTWKEILKDLEWEIEIDITGENKDK
                     QTAMATLNTVFQVIGRNPAVLQDENAKMLFNKILNLTGTISPLEIKEAQINNQQPSAP
                     ASIPDIREAQPQTVNQ"
BASE COUNT          493 a          585 c          504 g          759 t
ORIGIN      
        1 ttagccgccg tgccgattgt ccgggcaacg ataacctgcg tggtatcagt gttggttgtg
       61 gcaatgacgg tcgtatgcgc cgttgtgccg gtgctatact cacttcccgc cgtgccggtg
      121 ccgttaatgg cctttttaag gttatccaaa aatattgcct cactggtttt ccacaaaacc
      181 tgattcgcaa ccgccgtcaa cccgaaagat tcggtcaact cttttaccgc cgtataggtt
      241 gtggtgttaa tcgttatcgt tgccgcggtt gtggcaacgc ctccggccat tgttgccgct
      301 ccccaagaca acgatgcatc gttatccgtg gtattgtatg agtttgccgc cgtgccgatt
      361 gtcctggcca ctaccaccag ggttgtcgcc gtcaaagtcg tggcaataac ggtggtatgg
      421 gcggccgtcc cgacataata ctcaaccccc atcgttccgc tcaaattgat tgcggctttc
      481 aaatttgcca gtgtatcggc ggcggtagac ccaatccgca catcataagc ctgctcggga
      541 gtttttttga aacgataagt ggtcgtgttg atggttaccg tttttccatc agtaacattc
      601 gtgccgttgc tggtaagcgt ttgtttcgcg tgagcggccg gtgcgcaagc accgctactg
      661 gtaagcgttt gttttgcgta atctgccggg gcgcaagctc ctgatgatgt tattttttgg
      721 gttgcatata ctgctggggc gcaagcccca ccagaagtaa gcttgccagt agccacagtg
      781 ctggcttccg tgccgtcaat cacttttaat gtccccgcgg tggtcgcggt agtaataatt
      841 ttttgaagtt ttccctcgcc gattttaagc gtctgtgttt cgctgacaac ttttgaggtt
      901 gcccccaaga tctcgtattg ttgtgccggt gttgccatag ttattgatta accgtctgcg
      961 gttgcgcctc ccgaatatcg ggaatagacg ccggcgcgga tggttgttga ttattgattt
     1021 gcgcttcttt aatttccaac gggctgattg ttcccgtaag atttaaaatt ttattaaaaa
     1081 gcatttttgc gttttcatcc tgcaaaaccg cgggatttct tccgataacc tgaaacacgg
     1141 tgttaagcgt ggccatcgcg gtttgtttgt ctttgttttc cccggtaatg tcaatctcaa
     1201 tttcccattc caaatctttc aaaatctctt tccaagtttt ttggtcaata tcatccggct
     1261 tataaaatct cttgccgccc atcgctccca acatctcttg caatgcttgt ttttcttgcc
     1321 caagaattgc ctgctggtca atgtctgttt cgccgtccaa taccatctgc acaacttttt
     1381 cgtttgctct tttaactgcc tgcgaactaa taaacatttc atcaattctg tcaactccat
     1441 aatcatccaa atccgccacg atctcgtctt tgtcatcaaa ttgttttttc aggtgcggaa
     1501 taataaattc acgcaacatt ttttcaattg ccagcccttt gttttcggcc atcaaatcaa
     1561 ataaagaatg tccctcggta agcaatgctt ctgtttgtcg ccacgccgtg cctgatggcg
     1621 gattgcttcc caacatactc tcggaaatcc cgttaatttt attgcccaac tcttcccatt
     1681 gccgtccgta attttcaaag acaatcgtgt tctgcggatt ggtcgggacc aacgtgagcg
     1741 gctggttcgc ctgatggatg ataaagtcgc cgtgttctat ggtctgcaat gcgttctggc
     1801 cttgaagatt gccatcgctg gtctgccata tcatcttgct cgccaaatca aggtaatcct
     1861 taactgattt ttgggtatga ttagtcatcc attgcgcctc aaacagattt ttcacgcttc
     1921 cccataacga gattgaaccg tcttcactgg ggataagcca agtaagcata tagacatcct
     1981 ttttttcctt gcctttgtaa agagtaaaat cgtcatattc gcctttttct tttttttctt
     2041 gaaaagaaat cacgtgcatc tgttgcgtgt atttgttttg gtctttttct tctccggtca
     2101 aaaaagatag cggcaaattc ccgtgaattt cataaagttt gatatagtta tttttttggt
     2161 ctttttgccg accatcggca tccgtgcgcg attgttgcgc ggcaattaac gcttccacca
     2221 tttcttggtc atagccctgg cgttgtttta attgtccggg cgtcaactca ataatttcaa
     2281 tctttggatt accatcaaaa tccaattcat cgcaatatat cctgttccac ggcaccacca
     2341 t
//
LOCUS       DAOQWE010000126         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_51650_length_2333_cov_3.450834, whole genome shotgun sequence.
ACCESSION   DAOQWE010000126 DAOQWE010000000
VERSION     DAOQWE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51650_length_2333_cov_3.450834"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..391)
                     /locus_tag="PKK37_03895"
     CDS             complement(161..391)
                     /locus_tag="PKK37_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81553.1"
                     /translation="MLEETIILAIVVGLTEVAKRVGLVGRIVPVFAVVAGIGVAFIAN
                     LGGAWDVALQGLIVGLTAIGAYEVGKTTVLGK"
     gene            complement(429..968)
                     /locus_tag="PKK37_03900"
     CDS             complement(429..968)
                     /locus_tag="PKK37_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNR81554.1"
                     /translation="MKKLSVPYPGCKREHITQGFHSGHQALDFAFAGCYGAFLTAPAR
                     CEVEKIITDNSFDGEFYAQFQKGYGILLRDLENPRRQYLYWHCLQIFPVMVGQEIQEG
                     QVIAQIGNSGLCYSGGVLVPLKNRGTKGSHLHFEVLFDGKYVNPLDYIDFASHPKLDK
                     IKTVQQFLIFMTNFIKGRK"
     gene            complement(1093..1347)
                     /locus_tag="PKK37_03905"
     CDS             complement(1093..1347)
                     /locus_tag="PKK37_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNR81555.1"
                     /translation="MEVGFTSREAWELMKRMYDYTCPACGRREPDIELTRDHIVAVSE
                     GGTSFVENLQPLCKHCNQEKGRQNIYYPPKITMENRAQYA"
     gene            complement(1402..1548)
                     /locus_tag="PKK37_03910"
     CDS             complement(1402..1548)
                     /locus_tag="PKK37_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81556.1"
                     /translation="MMKIPWCRANAMAIPDWMAEDCNQKQCAYIVYVCERDVVDNYKK
                     QGGI"
     gene            complement(1651..1959)
                     /locus_tag="PKK37_03915"
     CDS             complement(1651..1959)
                     /locus_tag="PKK37_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81557.1"
                     /translation="MEQPAFITNERLNEFCKHQQAIEDKLSEVADDLKELKDAFNDQA
                     KEYKNICALIGEVPQGKSVMQCVRENANRTEKMWWAMIVVLGVIEFLANFPKLAGLFV
                     "
     gene            complement(1961..2176)
                     /locus_tag="PKK37_03920"
     CDS             complement(1961..2176)
                     /locus_tag="PKK37_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81558.1"
                     /translation="MQKNKIAIIVLSVLLVCLAGYVAYGEGVKLYNKQINQAYTKGVV
                     DVFGVVKDKGQISIQLDDGTITLIQKQ"
BASE COUNT          571 a          620 c          501 g          641 t
ORIGIN      
        1 aaacgggaag ctcgcgccaa aagtaaatcc tgctttggtc agtcgccgct tggctcgtat
       61 ttaccgccaa catcaacccg gcgaacgcca aagctaaata aaagacaatt tgtatttatt
      121 cataggtgta aaataggtat aaaataggta taattatacc ttatttcccc aagacggtgg
      181 tctttcccac ctcatacgcc ccgatggccg tcagacccac aatcaagccc tgcaaggcca
      241 catcccacgc cccgccaagg ttggcgataa aggcaacgcc aatgcccgct acaacggcaa
      301 agacaggcac aatacgcccc accagaccga ctcttttggc gacttcggtc aacccgacaa
      361 caatcgccaa gattattgtt tcttccaaca ttgtttttcg cgcttccgtt tttagcggat
      421 ggcgcggatt attttcttcc tttaatgaaa ttggtcataa aaatcaagaa ttgttgcact
      481 gtcttgattt tgtccagttt tggatggctg gcaaaatcaa tatagtccag cgggttgaca
      541 tatttcccgt caaacagaac ttcaaaatgc aagtggcttc ctttcgtgcc tctgtttttc
      601 agtggcacca gcacgccgcc ggaatagcac aacccgctgt tcccgatttg agcaataact
      661 tgcccctctt gtatctcttg ccctaccatc acaggaaaga tttgcaaaca atgccaataa
      721 agatattgtc ggcgcggatt ttcaaggtcg cgcaataaaa ttccatagcc tttttgaaat
      781 tgagcataaa attctccatc aaaagaattg tcggtgatta tcttttccac ctcgcaccgc
      841 gcgggtgcgg tcaaaaacgc gccataacat ccggcaaagg caaaatcaag tgcctgatgg
      901 ccggagtgaa atccttgcgt gatatgttct cgcttgcacc ccggataagg aacagatagt
      961 tttttcatat tttacttgtg ttaaagggat aagcaagaca gcgccggcta tgcaccttgc
     1021 ggccaatcgc cgggcctccc gcttttcccg ccggccgggg tcgactccgg caagcgggaa
     1081 aaacggggaa aatcaagcat attgcgcccg attttccatt gttattttcg gcggataata
     1141 gatattctgt cgtccttttt cttgattgca gtgcttgcac aacggttgca gattttccac
     1201 gaatgaagtc ccgccctcgc tcacggccac aatatggtcg cgcgtgagtt ctatgtccgg
     1261 ctccctgcgg ccgcacgcgg ggcaagtgta gtcatacatc cgtttcatca gctcccaggc
     1321 ttcccgggag gtgaaaccca cctccacgcg ccggtgccgc tttttgggtt tgccatcctc
     1381 tctggttttt ttgcttctga ttcatatccc tccttgtttt ttgtagttgt ccacaacatc
     1441 gcgttcgcac acatagacaa tgtatgcgca ttgcttctgg ttgcaatcct cggccatcca
     1501 gtcggggatc gccatcgcgt tggctctaca ccacggtatc ttcatcatag tccgcctcct
     1561 tttgataagg tgctactccc cggcgcggga aaatatgagt gccccaaaaa tgaattaaaa
     1621 aggcaaaaac ccgcgcggga gagaaacacc ctaaacaaac agtccggcca gtttcgggaa
     1681 attggccaaa aactcaatca cgcccaagac cacaatcatc gcccaccaca tcttctccgt
     1741 ccggttggcg ttctcgcgga cgcactgcat aacgctcttg ccttgcggga cttccccaat
     1801 caaagcacag atatttttgt attctttggc ctggtcgtta aacgcgtctt ttaattcttt
     1861 gaggtcgtcg gccacctcgc ttaatttgtc ctcaatcgct tgctggtgtt tgcaaaactc
     1921 attgagccgc tcgttggtga tgaatgccgg ttgttccata ttattgtttt tggattaaag
     1981 taattgttcc atcgtccaac tgaatggaga tttggccttt atcttttacc acgccaaaca
     2041 catcaaccac gcccttggta taggcttgat tgatttgttt gttgtagagt ttcaccccct
     2101 cgccgtaggc gacatagccc gcaaggcaga ccagcaaaac actcaaaacg ataattgcga
     2161 ttttgttttt ttgcatatta ttgtgtgatt attattcggc gagaagtttc ccctcccccc
     2221 gccgtgtagg tggcgtagat ggagaatgat ttatttgaat aatcaaagcc atctgttgct
     2281 ccatcagggg ttgaataact atcattaaat acatttccag tttgaccaga aac
//
LOCUS       DAOQWE010000127         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_51682_length_2332_cov_3.754941, whole genome shotgun sequence.
ACCESSION   DAOQWE010000127 DAOQWE010000000
VERSION     DAOQWE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51682_length_2332_cov_3.754941"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKK37_03925"
     CDS             complement(<1..561)
                     /locus_tag="PKK37_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013825361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81559.1"
                     /translation="MNLSDNEKRDIAKFIEAGKPLPDKYRFLLFEDKREVELVWNGKT
                     NEVTNVVLPFQTIEQVDEPRSEKEIKMQRSLFDIDSRGRQLKGWTNKLIWGDNKLILS
                     SLKNGSMREEIEKQGGIKLIYIDPPFDVGADFSMNIEIGNPENNGASEIFTKRPNVLE
                     ELAYRDTWGKGADSFIAMIYERLSLMR"
     gene            complement(779..1015)
                     /locus_tag="PKK37_03930"
     CDS             complement(779..1015)
                     /locus_tag="PKK37_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81560.1"
                     /translation="MSQQELAKKAQTTQKRISNIENADVDLGMSAIYRISQALCFDSK
                     TLADIFGCDFNPLQAQSPEIESRQFQESVFCGKS"
     gene            complement(1012..1152)
                     /locus_tag="PKK37_03935"
     CDS             complement(1012..1152)
                     /locus_tag="PKK37_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81561.1"
                     /translation="MKITNKKNIDPIAEARKNKHFKRYSDEADVKIRFAVAVYDAELK
                     SG"
     gene            complement(1136..1450)
                     /locus_tag="PKK37_03940"
     CDS             complement(1136..1450)
                     /locus_tag="PKK37_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNR81562.1"
                     /translation="MALIAAIDSKIIFLSRQKSHPHPPISKSLHNYNFFEIRHRKNRN
                     ILIRVIYFCCEGKMILLNAFEKPDNYDSQKIKKDIEKIFDTTAMYRQKYLKNKNLYED
                     YK"
     gene            complement(1660..2145)
                     /locus_tag="PKK37_03945"
     CDS             complement(1660..2145)
                     /locus_tag="PKK37_03945"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNR81563.1"
                     /translation="MSKNIFVVSLVVVAVGGGAFFGGMKYQESKSASAFPAARNFQGM
                     PGAAAGDSGARANRNGGGGMVVGEITAVDGDSVTVKSADGASKIVFFSEKTSIGNFTA
                     AQSGDLAVGQSVTAAGTAGTDGSIVAGNIQIGAPARSAGTPPAAPADENQEEPAVQPV
                     M"
BASE COUNT          598 a          549 c          436 g          749 t
ORIGIN      
        1 gcgcatcagc gaaaggcgtt cgtaaatcat ggcgataaaa ctgtccgcgc ctttgcccca
       61 ggtgtcgcga taggccaatt cttccaaaac attcggtctt ttggtgaata tctcgcttgc
      121 cccgttattt tcggggttgc cgatttcaat gttcattgaa aaatccgcgc ccacatcaaa
      181 cggcggatca atataaatca atttaatgcc gccttgtttt tcaatttcct cgcgcattga
      241 gccatttttc aaactggaaa ggattaactt gttatcgccc cagatcagtt tattcgtcca
      301 gcccttcaat tgccgcccgc gcgaatctat atcgaaaagc gatctttgca ttttaatttc
      361 cttttcactg cgcggctcgt ccacctgctc aattgtttga aacggcaaaa ccacattggt
      421 aacttcgttg gtttttccgt tccacaccaa ttccacttcg cgcttatctt caaacaacaa
      481 aaaccggtat ttatccggca acggctttcc cgcctcaata aatttagcta tatcccgttt
      541 ttcgttatcc gaaagattca tgcgctttat atctctattt tacatataca ttacctcttt
      601 tgcgatcatt tttcaatttt taaccaaaca atgccatttg actgataatt tatgcaaaag
      661 cgcggcgttt atgccgcgcc caacgcttaa ttaccgatta ggccattcca aacgcaaatt
      721 atgatgcttt atcaattcta aatttgcaac gaccagcttc gcgaaaattt cgtttttctt
      781 aacttttgcc gcaaaacacg gattcttgaa attgccggct ttcaatctcg ggagattggg
      841 cttgcagagg attaaagtcg catccgaaaa tatcggctaa cgtttttgag tcaaaacaaa
      901 gcgcttgcga tattctgtaa atcgcactca tacccaaatc aacatccgca ttttctatgt
      961 ttgatattct tttttgtgtg gtttgcgctt ttttggccaa ttcctgttga ctcatccgct
     1021 ttttagttcg gcatcataaa ccgcaaccgc aaatctgatt ttcacatccg cttcgtcgga
     1081 atatctttta aaatgcttgt ttttcctggc ttcggcgatg gggtcaatgt tttttttatt
     1141 tgtaatcttc ataaagattt ttatttttta aatatttttg acgatacatt gcggttgtat
     1201 caaaaatttt ttcaatatct ttctttattt tttgcgaatc ataattatcc ggtttctcaa
     1261 aagcatttaa taaaatcatt ttaccttcac aacaaaaata aattactcta ataaggatat
     1321 ttctgttttt tctgtgccga atttcaaaaa aattataatt atgcaatgat tttgatatcg
     1381 gcggatgcgg atggcttttt tggcgactca aaaaaatgat tttgctatca atggccgcaa
     1441 ttaatgccaa atttttggca acgccaagat caaattgatt taaatatcgc tttaccggac
     1501 aggattttaa atccggatca taataataaa caatattcat aatttacgcc gcctcagtta
     1561 atacattata tcttattgga tataattgtc aatatcaaac cggatttgca aaaaacgcgg
     1621 catttatgcc gcgctttttt tgggagttcg ggcgaagttt tacattacgg gctggacggc
     1681 gggttcctcc tgattttcat cggccggagc ggccggcggg gttccggcgg aacgcgccgg
     1741 cgcgccgatt tggatgttgc cggcgacgat actgccgtcc gtgccggcgg ttcccgccgc
     1801 cgtcaccgac tggccgaccg ccagatcgcc cgattgcgcc gcggtaaagt tgccgattga
     1861 ggttttttcg gaaaagaaaa cgatttttga cgcgccatcc gcggatttga cggtaacgct
     1921 gtcgccgtca acggcggtga tttcgccgac aaccattccc ccgccgccat tgcgattggc
     1981 gcgcgcgccg ctatcccccg ccgcggcgcc gggcattccc tgaaaattcc ttgccgccgg
     2041 aaacgcagac gcgcttttgc tctcttgata cttcattccg ccaaaaaacg cccctccccc
     2101 aaccgctaca accaccaatg aaacaacgaa tatatttttg ctcatgtttt aaattaaaaa
     2161 ttgaaaatga aaaggtaaaa ataaatttta aattgaaata atttttaatt ttacattttc
     2221 gtttttcatt tttaggtttt aatttttaat ttttgtcatt catatctcag tgccgtgatc
     2281 ggatccattt tggccgcgcg cgcggcggga taataaccga aaacgatacc aa
//
LOCUS       DAOQWE010000128         2329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_51778_length_2329_cov_6.307828, whole genome shotgun sequence.
ACCESSION   DAOQWE010000128 DAOQWE010000000
VERSION     DAOQWE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51778_length_2329_cov_6.307828"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKK37_03950"
     CDS             complement(<1..751)
                     /locus_tag="PKK37_03950"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR81564.1"
                     /translation="MLFLTQQLIDAGVVTAEQAAKVEAELAKDSGRTEEEVIIMQGFV
                     EEKKLFELKSRWLGISLIVDPPEDLSLEILETIPEETAKFYRMIPLGKKKSEFMVGMV
                     CPEDLKAQEALKFLARKNSFIYRVFLITPTILTGVLAKYRDLKKEVGRALEEFQEEES
                     QNKVSRQKQQEETISGVESAPIAKIVNVILRNAIDGNASDIHIEPSRTKLRVRFRVMG
                     DLFSSIFLPLNIANAVLARIKIMSNMKIDENR"
     gene            complement(835..1137)
                     /locus_tag="PKK37_03955"
     CDS             complement(835..1137)
                     /locus_tag="PKK37_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81565.1"
                     /translation="MAITFQEKSNLGKKIAVLLVLFLAFVAIVFFGWRFIEQSSPVPS
                     ALPASVPALNIEALQDPRLAEMEMFPRISPPEEEIARGNPFAEVSATTTADTAGED"
     gene            complement(1181..1783)
                     /gene="pilO"
                     /locus_tag="PKK37_03960"
     CDS             complement(1181..1783)
                     /gene="pilO"
                     /locus_tag="PKK37_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNR81566.1"
                     /translation="MSRNYLEAFAILIIAFALGFFLLLPKTQELRDLQARVQSKIGEL
                     KERGEYYAALRAMVDDLSYHREGLEKIGTALPEGADAAAVMDFAQTAAMHSGLSVKDV
                     EYSGTAAGTDTGADPTSPEASLKNYTISLKLSGSYGNFKSFLAVIENSSRLISVESLD
                     IDSKSEIERKGESPEEKSAPLAAEGEKILDYDVKLSTNYY"
     gene            complement(1829..>2329)
                     /locus_tag="PKK37_03965"
     CDS             complement(1829..>2329)
                     /locus_tag="PKK37_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81567.1"
                     /translation="GLFFENVVFYFSFILLALAAGAFFYVRHLASSAAAELSNLEVQM
                     AEMKTEEQRKLENDVNAARRRLDDFSRIISARNLSRDLFAKLENLIVPGVHLSKMSAD
                     FAEMSAELSGHGNNFSEMERQAMKFRNSPEILKSANLEKISTVETGGVDFGATAVFNS
                     ETEKTE"
BASE COUNT          601 a          638 c          507 g          583 t
ORIGIN      
        1 cgcgattctc gtcaatcttc atatttgaca ttattttgat gcgcgccagc accgcgttgg
       61 caatgttaag cggcaaaaaa atcgacgaaa agagatcgcc catcacgcgg aagcggaccc
      121 tcaatttcgt tcggctgggc tcaatgtgaa tgtcggaagc attgccgtca atcgcgttgc
      181 gcaaaatcac gttaacaatc ttggcgatcg gcgcgctttc cactccggaa attgtttctt
      241 cctgctgttt ttgccgcgaa actttgtttt gcgactcttc ttcttggaat tcttccagcg
      301 cccgccccac ttcctttttt aaatcgcggt atttagccaa aacgccggtg aggattgtcg
      361 gcgtgattaa aaacacccgg tagataaagc tgtttttccg cgccagaaac ttaagcgcct
      421 cctgcgcctt cagatcttca gggcaaacca tccccaccat aaattcgctc tttttctttc
      481 ccagcggaat catccggtaa aatttggcgg tttcttcggg gattgtctcc aaaatttcca
      541 gagaaaggtc ttcgggagga tcaacgatca acgaaatgcc cagccatcgg ctttttagtt
      601 caaaaagctt cttctcttca acgaagcctt gcatgatgat tacctcttcc tccgttctcc
      661 ccgaatcttt cgccaactcg gcctccacct tggcggcttg ctcggcggta acgacaccgg
      721 catcaataag ttgttgcgtt aaaaacagca ttggaaattt aaaacatctt taggcgccac
      781 taaaaaccgc cggaggcaac gaggcggcca gaccgtgcat ctgccctttc aacgctaatc
      841 ctcgccggcg gtatcggcgg tcgtggtcgc cgaaacctcg gcaaaaggat ttccccgcgc
      901 aatttcttcc tccggcggcg aaatgcgggg aaacatttcc atctccgcca gccgcgggtc
      961 ttgcaacgcc tcaatattga gcgcgggcac ggaagcgggc aaagcggaag gaacggggga
     1021 actttgctca ataaaccgcc aaccgaaaaa aacgattgca acgaacgcca aaaacaaaac
     1081 aagcaaaacc gcgatctttt ttcccaaatt gcttttttcc tgaaaagtta ttgccatatt
     1141 aaaagattag acctactgcg taaatattta cgcgacaagc ctaataataa ttcgttgata
     1201 atttaacgtc gtaatccaga atcttttccc cctccgccgc caacggcgcg gatttttctt
     1261 ccggcgattc gcccttcctt tcaatttccg attttgaatc aatgtccaga gattccacgg
     1321 agatcaagcg gctggaattt tcaatgaccg ccaaaaaact tttgaagtta ccgtaactgc
     1381 cgctcaattt gagcgaaatg gtatagttct tcaaagatgc ttccggcgat gtcggatcgg
     1441 cgccggtatc ggtgccagcg gcagtaccgg aatattcaac atccttcacc gataaacccg
     1501 aatgcatcgc cgcggtttgc gcaaaatcca tcaccgccgc ggcatcggcg ccttcgggaa
     1561 gggcggtgcc gatcttttcc aaaccttcgc ggtgataact caaatcgtcc accatcgccc
     1621 tgagggcggc ataatactct ccgcgctctt taagttcgcc gattttgctt tgaacccggg
     1681 cttgcaagtc ccgcagttcc tgggttttgg gaagcaggag aaaaaaaccc aaggcaaaag
     1741 cgataatgag aatcgcaaac gcctccaaat agtttcttga catagacgtt attattttgt
     1801 tagaaatcaa aatttgaaaa cgcgaagatt actccgtttt ttcggtttcg gaattaaaaa
     1861 ccgcggtggc gccaaaatca acgccgccgg tttccacggt ggaaattttt tccaaattgg
     1921 ccgatttcaa aatttccggc gaatttctga atttcatcgc ctgccgctcc atttccgaga
     1981 aattattccc atgcccggaa agctccgccg acatctccgc aaaatcggcg ctcatttttg
     2041 acaagtgaac gccgggaaca atcaaatttt ccagcttcgc gaaaagatcg cgcgacaaat
     2101 tgcgggcgga aattatcctt gaaaaatcat ccaaccgccg gcgcgccgcg ttaacgtcat
     2161 tttccaattt tctttgttct tcggttttca tttccgccat ttgcacttcc aaattggaca
     2221 attccgcggc cgctgacgac gccagatgcc ggacatagaa aaacgcgccc gccgccagcg
     2281 ccagcaaaat gaaggaaaag taaaagacca cattctcaaa aaaaagccc
//
LOCUS       DAOQWE010000129         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_52965_length_2295_cov_7.564286, whole genome shotgun sequence.
ACCESSION   DAOQWE010000129 DAOQWE010000000
VERSION     DAOQWE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52965_length_2295_cov_7.564286"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..701)
                     /locus_tag="PKK37_03970"
     CDS             complement(78..701)
                     /locus_tag="PKK37_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81568.1"
                     /translation="MKNKLNRKNIVAAAVMVFCAAVAVWGGAAYADEIAIKENPMSGL
                     AAVIASKFNLSESAVQAVFDEQMQARRERMEANRKEMETKRREQFEDRLNKLVSEGKL
                     TEAQAAAIKNKKAEMKAQMTAQAGAKFDLKNMTVEERKAAMEKQKTEMEARRAALEQW
                     AADNGIAEEYLPMVGAGFGGGGHGRGGGFGGPGFGGQGPCSDNVPES"
     gene            896..2287
                     /locus_tag="PKK37_03975"
     CDS             896..2287
                     /locus_tag="PKK37_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81569.1"
                     /translation="MTKGFRTFMFAFFLLGFAVAAPAIALYAQGYRLNWPIEPGKKLI
                     VKTGGFFAKAYPKQIDVYVNGRAEKQTDFFFDSALVTNLLPRQHLFEIKKTGYQTWAK
                     NLEVREKEVVEAKNIFLFPELPQFKTLEENIADFSVSGDRQKIALRHEDGTSWNIKLY
                     DIGRAVTAKLAGQSNFALKDPEFSGWTWIDKNTIEISAKSKDASETYLIAIDKNPPQL
                     VKKIPPEEEPAGKEEPEEISAVSVAPAAGENAAEEKPGAPRELARGQNGGADYYLADD
                     GFIYNQENGGEPALAAAGRIDVAAEAGYKLEIFGDYFFVAAEDRLMAIRKDGKEFEKI
                     LDSAPRGTKMAPNGKRVVYWSNSEIWIFHLEKKTDPPAAEAGEKTFIARFSEKITDCD
                     WFNSDYLIFTAGDRVRTAEIDNRDKINFAEIAEFSKIGGENSSEGKMFMDQDKKILYL
                     LAGKTLRQTNLYE"
BASE COUNT          584 a          607 c          542 g          562 t
ORIGIN      
        1 caaacaaaaa cccgccgggt tttgccggca gagttttcgc tctttgtttt gcgctttaat
       61 ttgcgccgtc ggttggatta tgattccggg acattatcgg agcaaggccc ctggccgcca
      121 aatcccggac cgccgaatcc tcccccgcga ccatgcccgc cgccgccaaa tcccgcgccg
      181 accataggca aatattcttc ggcaatgcca ttgtccgccg cccattgctc cagcgcggct
      241 ctccgcgcct ccatctcggt tttttgtttc tccatggccg ccttgcgttc ctcaacggtc
      301 atatttttaa gatcaaattt cgcgccggct tgagccgtca tttgcgcttt catctccgct
      361 tttttattct tgatcgccgc cgcctgcgcc tcggtcaatt ttccttcgga aaccagcttg
      421 ttcaaccgat cttcaaattg ttcccgccgt tttgtttcca tttccttgcg attggcttcc
      481 attctttcgc gccgcgcctg catttgctcg tcaaacaccg cttgcacggc actttcgctc
      541 agattaaatt ttgaagcgat aaccgcggcc aatccgctca ttggattttc tttgatggca
      601 atctcatcgg cataggccgc gccaccccaa accgcaacgg ccgcgcaaaa caccatcacc
      661 gccgcggcga caatattttt tctgtttaat ttgtttttca taattttttg tttttagatt
      721 ttagatttgc cgtataatta cggcaatttt catttttctt tgcgttgtcc ggaccggcaa
      781 taaattatta caaccgcgcc ggggtaatat ttcattggaa aacggcgggc atcatgagaa
      841 cccgttgaaa tatcggcgca ataagctatg atgaagacat tgaaataccg ttaaaatgac
      901 caaaggtttc cgaacattta tgtttgcgtt ctttttgctg ggattcgccg tcgcggcacc
      961 ggcaatcgcg ctctacgcgc aaggataccg cctgaattgg ccgattgaac cgggcaaaaa
     1021 attgattgtt aaaaccggcg gttttttcgc aaaagcctat cccaagcaaa ttgacgtcta
     1081 tgtcaacggg cgcgcggaaa aacaaaccga ttttttcttt gattcggcac tggtcaccaa
     1141 tcttttgccg cgccagcatt tgtttgagat caaaaaaacc ggctaccaaa cctgggcgaa
     1201 gaacttggaa gttcgcgaga aagaagtggt ggaggcaaaa aacatttttt tgtttcccga
     1261 attgccgcag ttcaaaacat tggaagaaaa catcgccgat ttttcggttt ccggcgatcg
     1321 ccaaaaaatc gctctgcgcc acgaagacgg gaccagctgg aacatcaagc tctatgacat
     1381 tggccgcgcg gtaacggcaa aacttgccgg acaaagcaat tttgctttaa aagaccccga
     1441 attttccggc tggacgtgga ttgacaaaaa cacgattgaa atttcggcaa aatcaaagga
     1501 cgcttcggaa acctatttga tcgccattga caaaaatccg ccccagcttg tcaaaaaaat
     1561 cccgccggaa gaagaacccg ccggcaaaga agaaccggaa gaaatttcgg cggtgtcggt
     1621 ggcgccggca gccggcgaaa acgccgcgga agaaaaaccc ggcgcgcccc gggaattggc
     1681 acgcgggcaa aacggcggcg cggactatta tttggcggac gacgggttta tttataacca
     1741 ggaaaacggc ggcgaaccgg ccttggccgc cgccggccga atcgacgtcg ccgcggaggc
     1801 cggatacaag cttgaaattt ttggcgatta tttttttgtc gccgccgagg atcgcctgat
     1861 ggcgatccgg aaggacggca aagaatttga aaaaattctt gacagtgcgc cgcggggaac
     1921 aaaaatggcg cccaacggaa aaagagttgt ttattggtca aattctgaaa tctggatttt
     1981 ccatttggag aaaaaaaccg atccgccggc ggcggaagcc ggcgaaaaaa cgttcatcgc
     2041 gcgcttttcc gaaaagatca ccgactgcga ctggttcaat tccgactatt tgatttttac
     2101 tgccggcgac cgcgtcagaa ccgccgaaat tgacaaccgc gacaaaatca attttgccga
     2161 aatcgccgaa ttttcaaaaa tcggcggaga aaattccagc gagggaaaaa tgtttatgga
     2221 ccaagacaaa aaaattctct atctgctggc cggaaaaact cttcgccaaa ccaatcttta
     2281 cgaatgaaac ggggc
//
LOCUS       DAOQWE010000130         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_53577_length_2278_cov_9.417004, whole genome shotgun sequence.
ACCESSION   DAOQWE010000130 DAOQWE010000000
VERSION     DAOQWE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53577_length_2278_cov_9.417004"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /gene="cysS"
                     /locus_tag="PKK37_03980"
     CDS             complement(<1..1308)
                     /gene="cysS"
                     /locus_tag="PKK37_03980"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNR81570.1"
                     /translation="MNKIKIYSTLSKSIEDLKPMEEGKIKFFVCGPTVYDFPHLGHAK
                     TYTQFDFIVKYLRWRGFEVNYLQNITDLDDKIIQRAAERGISWRQLADDYEKIYKDDM
                     LALHNTAVSKFARATDYIENIVSQVKRMIDKNLAYQAPDGSWYFEIAKFKDYGKLSGR
                     TELKETDGVSRIDESDFKRGWNDFCLWKSSKEGEPSWETELGKGRPGWHIEDTAITEV
                     EFGPQYDIHGGAIDLIFPHHEAEITQIESITGKVPFVRYWMHAGFLNINAQKMSKSLG
                     NFKTIRDVLKQYDYRVLRYFFISNHYRAAVDFSDAGLDQAKNSLARIDEFVFNKVDAA
                     YDDNADEAVVNETKEKFCAALDNDLNTPQAFAALFDFIRAQNQKEKSGKRTFEFLNQV
                     NTFLGFMQFDQAKSDEFIESLVKERDAARAAKDFAKSDQIRDRL"
     gene            complement(1326..>2278)
                     /locus_tag="PKK37_03985"
     CDS             complement(1326..>2278)
                     /locus_tag="PKK37_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR81571.1"
                     /translation="TETMAYLNSQLQKRIDEKFYRLQGLRPLPVDKVKKLREQFAVEM
                     TYNSNAIEGNSLTLKETFFVISEGITIKGKPLKDHLEAKDHQEALGYLYDLADQKKQS
                     DILEMTIRNLQKLVVKETASDIAGSYRTGNVIIGGASHCPPDAFEVPNLMRNAVNWIN
                     ANEGKMHPVEFAALAHHKIVYIHPFFDGNGRTARLLMDLLLMRAGFPLAVVLKNDRKK
                     YYQTLEQADKEKFSPFVDFIARAVERSLDIYLNVLLPVTSKNQKYFSLSEIAKSTPYS
                     EKYLNLLARQGKIDAHKDGRNWRVSLGSIKNYIKNRQRQR"
BASE COUNT          573 a          566 c          458 g          681 t
ORIGIN      
        1 caggcggtcg cgtatctggt cggacttggc gaaatctttc gccgcgcggg cggcgtcgcg
       61 ctccttgacc agcgattcaa taaattcgtc gctttttgcc tgatcaaatt gcataaatcc
      121 caaaaaggtg ttgacttggt ttaaaaactc aaatgtccgt ttgccggatt tttctttttg
      181 gttttgcgca cggatgaaat caaacagcgc ggcgaacgcc tgcggagtgt tcaaatcgtt
      241 gtccagcgcg gcgcagaatt tttcttttgt ctcgttgaca accgcttcat cggcgttatc
      301 gtcataagcc gcgtcaactt tgttgaaaac aaattcatca atgcgggcca aagaattttt
      361 tgcctgatcc aacccggcat cggaaaaatc caccgccgcg cggtaatgat tggaaataaa
      421 gaaataacga aggactcggt agtcatattg tttcagaaca tcacggatgg ttttgaaatt
      481 tcccagcgat ttggacattt tttgcgcgtt gatgtttaaa aatccggcgt gcatccagta
      541 gcgcacaaac ggcaccttgc cggtgattga ttctatttgc gtgatctcgg cttcatgatg
      601 ggggaaaatc agatcaatcg cgccgccgtg aatgtcatat tgcggaccga attcaacctc
      661 ggtgatggcg gtgtcttcaa tgtgccagcc gggccggccc ttgcccaact cggtttccca
      721 tgacggctcc ccttctttgg atgatttcca caaacaaaaa tcgttccacc cgcgcttgaa
      781 atcgctttcg tcaatgcgcg acacgccgtc ggtttccttc aattcggtgc gtcccgacag
      841 tttgccataa tctttgaatt tggcaatctc aaaataccaa ctgccgtcgg gcgcctgata
      901 ggccaaattt ttgtcaatca ttcgcttcac ctggctcacg atattttcaa tgtagtccgt
      961 ggcccgcgcg aatttgctca ccgccgtgtt gtgcagtgcc agcatatcgt ctttgtaaat
     1021 tttttcataa tcatcggcca gttggcgcca gctgattccc ctttccgcgg cccgttgaat
     1081 gattttgtca tcaagatcgg tgatgttttg caaataattc acctcaaacc cgcgccagcg
     1141 caaatatttg acgataaaat caaattgcgt gtaggttttg gcatgcccca aatgcggaaa
     1201 atcataaacc gtcggcccgc aaacgaaaaa tttgatcttc ccctcctcca tcggctttaa
     1261 atcttcaatg gacttggaaa gcgtgctgta tatttttatc ttattcatat tatttttgtt
     1321 aaatattacc tttgacgctg tcgattttta atgtaatttt tgattgaccc taaagaaacc
     1381 cgccaattgc gtccgtcttt atgcgcgtca attttcccct gccgcgccaa taaattcaaa
     1441 tacttttccg aatatggtgt tgacttggca atctcggaaa gtgaaaaata cttctggttt
     1501 tttgaagtta ccggcaacaa cacatttaaa taaatgtcta aagaccgctc cacggcgcgg
     1561 gcgataaaat caacaaatgg agaaaatttc tctttatcgg cttgttccaa tgtctgataa
     1621 tatttttttc ggtcattttt taaaaccacc gccaaaggaa accccgccct catcaacaac
     1681 aaatccatta acagtcgcgc cgtccggccg ttgccatcaa aaaacggatg gatataaacg
     1741 attttatgat gcgccaacgc cgcaaactca accggatgca ttttaccctc gtttgcatta
     1801 atccaattaa ctgcgttgcg cattaaattc ggcacctcaa acgcatccgg cggacaatga
     1861 ctcgcgccgc caataatcac attgccggtg cgatatgaac cggcgatatc cgacgccgtt
     1921 tctttaacca ccaatttttg caaatttctg attgtcattt ccaaaatatc gctttgcttt
     1981 ttttgatccg ccaaatcata cagatagccc aacgcctctt gatgatcttt ggcttccaag
     2041 tgatctttta gtggttttcc tttaattgtg atcccttcgc taataacaaa gaatgtttct
     2101 ttcagtgtca gagaattgcc ttcgatcgca ttggaattgt aagtcatttc aaccgcaaac
     2161 tgctctctca attttttcac tttatcaacc ggaagcggcc gcaacccctg caagcggtaa
     2221 aatttttcat caattctttt ttgtaactgc gaattcaaat atgccatagt ttcagtat
//
LOCUS       DAOQWE010000131         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_53789_length_2272_cov_11.905277, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000131 DAOQWE010000000
VERSION     DAOQWE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53789_length_2272_cov_11.905277"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..177
                     /locus_tag="PKK37_03990"
     tRNA            91..177
                     /locus_tag="PKK37_03990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:125..127,aa:Leu,seq:taa)
     gene            187..882
                     /locus_tag="PKK37_03995"
     CDS             187..882
                     /locus_tag="PKK37_03995"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI family restriction endonuclease"
                     /protein_id="HNR81572.1"
                     /translation="MPLKKFSDFLESIDLKFYRDKYRLIKIVEMDLPKEIQAIDLLYK
                     IYWNQKEFLSFEKFYKEYLKEYNRNVKNFQEKTGMCEKCFYKGLPARIYRTWASIITQ
                     IHAGYVAESVFGKNTVTMSCELDHKGADFQVRYKNKILNYQVKKKSLGREVRQEKPKS
                     KNQLPGEFIDIRYEVPSSDYFENPKRKDGKYKLPYERFQNNKELKRLLNGFVVFTPYA
                     FEEKKRELDKTSK"
     gene            complement(874..2043)
                     /locus_tag="PKK37_04000"
     CDS             complement(874..2043)
                     /locus_tag="PKK37_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR81573.1"
                     /translation="MLTRRQKEVLDFVENYLTKKGYAPSFDEIRRRLKVASVSTVDFH
                     ISKLEEGGYLEKTKNKARTINIVKKPILEYPLLVKEGCAAVEEFIITEEIKKERKLPL
                     NQVILGDAIQELRKLPDESCDIVVADPPYNIGKDFGNNIDKREIGEYVAWCKIWINES
                     IRALKPNGTMFIYGFSEILAFLSIEIPIQKRWLIWHYTNKNVASLNFWQRSHEAIICA
                     WKNKPIFNRDEVREPYTESFLNGAAGKVRKGTLGRFSGKGMETVYNAHKGGALPRDVI
                     KIPALAGGAGMVERWFLCKTCDGVYEPRQLKNHEGHEIMKHPTQKPYELSKKLIKSAM
                     PKKNGVVLVPFVGTGSECAAAKELGQSYIGFEINPDYVRLAEKMISDKKPIAKLF"
BASE COUNT          705 a          499 c          382 g          686 t
ORIGIN      
        1 aaaattaagg taaaattcct tacggaattt tgattataat ttttaatttt aaattgtcat
       61 tttgattttt tcaaatttta atttttatac gcccgggtgg tggaatcggc atacacgagg
      121 gacttaaaat cccttgggag caatctcgtg tgggttcaag tcccaccccg ggcaccaaat
      181 aaaaatatgc cgttaaaaaa attcagcgac tttttagaat cgattgatct caaattctat
      241 cgcgacaaat acagacttat taaaattgta gaaatggatt tgccaaaaga aattcaagcc
      301 attgatttgc tttataaaat atattggaac caaaaagaat ttttaagttt tgaaaaattt
      361 tataaagaat acctgaaaga atataaccgg aatgtcaaaa atttccaaga aaaaaccgga
      421 atgtgcgaaa aatgctttta caaaggatta ccggcaagaa tttacagaac gtgggcaagc
      481 attatcactc aaatacacgc tggttatgta gccgaatctg tttttggcaa aaacacagtc
      541 acaatgtcgt gcgagctcga tcataaggga gcggactttc aagtaagata taaaaacaaa
      601 attttaaatt accaagttaa gaaaaaatct cttggcaggg aggttcgtca agaaaaaccg
      661 aagtcaaaaa accaattgcc gggagaattt atagacatca gatatgaagt cccaagtagc
      721 gattattttg aaaatccaaa aagaaaggat ggtaaatata agctccccta tgaaagattc
      781 caaaataaca aagaattgaa aagactgctg aatggtttcg ttgtttttac accctacgct
      841 ttcgaagaaa agaaaaggga attggataaa acttcaaaat aattttgcga ttggcttttt
      901 atcgcttatc attttttccg ccaatctcac ataatccggg tttatttcaa aaccaatata
      961 cgattggcca agttcttttg ccgccgcgca ttccgatccg gtgccgacaa aaggcactaa
     1021 tacaacgccg tttttcttcg gcattgccga ttttatcaat tttttggaca actcataagg
     1081 tttttgcgtg gggtgtttca ttatttcatg cccctcatgg ttttttaatt gtcgcggctc
     1141 ataaacgcca tcacaggtct tacataaaaa ccatcgttca accattccgg cgccaccggc
     1201 aagcgcggga attttgatta catctcgcgg taaagcccct cccttgtgcg cattataaac
     1261 tgtttccatc cccttgccgc taaaccgccc caaggttccc tttcgcactt tcccggcggc
     1321 tccgttcaaa aaactctccg tatacggctc cctcacttca tcacgattaa aaatcggctt
     1381 gtttttccat gcacaaatta tcgcctcgtg acttctttgc caaaaattta acgacgcgac
     1441 attcttattt gtatagtgcc aaatcaacca tcgcttttgt atcggaattt caatggaaag
     1501 aaaggccaaa atctcgctga aaccataaat gaacattgtt ccgttcggct ttaacgctct
     1561 tatgctttca tttatccaaa tcttgcacca cgccacatat tccccaattt ctcgtttatc
     1621 aatattgttg ccaaaatcct tgccgatatt ataaggcgga tcggctacaa caatgtcgca
     1681 actttcatcg ggcaattttc ttaattcttg aatagcatca cccaaaatca cttgatttaa
     1741 cggtaatttc ctctcttttt tgatctcttc ggttattata aattcttcaa ctgccgcgca
     1801 cccctctttt accaaaagcg gatattccaa tatcggtttt ttgacaatat tgatcgttcg
     1861 agccttattt tttgtctttt ccaaataacc accctcttca agttttgaga tgtgaaaatc
     1921 aacagtcgaa acagaagcga ctttgagccg cctcctgatc tcgtcaaagg agggcgcata
     1981 ccctttcttt gtcaaataat tttccacaaa atcaagaacc tctttttgtc gccttgtgag
     2041 cataattttt aaaatatttt tatcacttcc cgtatgttta ttatactaga acaaaaatag
     2101 aaaatcaaag cccggttatc cacagccggt acgcaacaaa aaaccggctc ttgaaaattc
     2161 aggtcggctt tgttttggga aattcaaagg gtctaaccct ctgaaaaccc tccgcaaaac
     2221 taccgcaccg gcttgggatc gctcaccccg ccctcttcgc aactgtaaat ga
//
LOCUS       DAOQWE010000132         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_55373_length_2231_cov_4.113051, whole genome shotgun sequence.
ACCESSION   DAOQWE010000132 DAOQWE010000000
VERSION     DAOQWE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55373_length_2231_cov_4.113051"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..>2231)
                     /locus_tag="PKK37_04005"
     CDS             complement(341..>2231)
                     /locus_tag="PKK37_04005"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNR81574.1"
                     /translation="FKKNIMALYYNLPIYKASYKLVTMLFANSSGFAREYRYTVGQDM
                     KNEGTSLIKNIYRANKAADKIADLSQARENLETIRLLTRLMQDFNQLSLKKFVEINQA
                     IEEVSKQLASWEKYSKSNTKPPESSVASAQASVRSKSNNPLAASKEFCPAFGHIAPLH
                     QKNGAINSEVIIPLAGNRNRASASLNNRGSNGNYWSGSPSGTNAANLNFNSSAVNPAN
                     TNNRANGFSVRCVKDLQKEIVKKQNNRQLLLDLFRAYFDARRNKRSTASALAFEAGYE
                     GKLFNLYEEIINREYKIGRSICFIVNKPVKREIFAADFRDRVVHHLIFNYINPIFEKH
                     FIKDSYSCRIGKGTAMGIRRADHFIRSGSENYQKDCWILKLDIKGYFMAMDRNILYQK
                     IEQRLKAAKNAEFDVDLALYLIREIVFHAPTKNFHIKGKREDWIGLPKSKSLFFAKNG
                     KGFPIGNLTSQLFGNIYLDELDHLVREKFGMKHYGRYVDDMILVHSDKEFLKGAVLKI
                     KDYLEKERGLKLHPRKTYLQHFKKGVCFLGTFVKPHRIYIGTPTKKNFYAMADEWNLT
                     ARKNRGLGDEETKKFISGVNSYLGLMKHYNTFKLREKTAQSFNEEIFKSIVFSEKFEK
                     AIVAA"
BASE COUNT          531 a          514 c          481 g          705 t
ORIGIN      
        1 caatgccgga ggccgaggtg ccggacaata aatcggcgga aacggcgacg gcctgaggga
       61 cataagtcgc ggcgaaagtt tggtatggca aaaacgccgc catcacggtc attgctaacg
      121 cgattaaatt atatttgcct cttatcttca tagcctttct aaaaatactt gctacctccg
      181 gataaccact ttatatatta tggcacattg ccataatatt tatttaattt tcaataaaat
      241 ttaaccttgt aatcttttcc atccgaagca gttgttaaga aattcttaat aactgaattt
      301 tcatctttct tattttttca tcgtgctcaa tttttattca tcaggcggcc acgattgcct
      361 tttcaaattt ttccgaaaaa acaatacttt taaaaatctc ttcattgaag ctttgcgccg
      421 ttttttccct caatttgaaa gtgttgtaat gtttcatcag ccccagatag gaattgacgc
      481 cggaaataaa tttcttggtt tcctcatcgc ccagcccccg gtttttccga gcggttaaat
      541 tccattcgtc ggccatggca taaaaatttt tctttgttgg cgtgccgata tagatccgat
      601 ggggcttgac gaatgtcccc agaaaacaaa cgcctttctt gaaatgctgt aagtatgttt
      661 ttcggggatg aagtttaagc ccccgctctt tttccagata atctttgatt tttaaaaccg
      721 cgcctttcaa aaattccttg tcggaatgaa ccaaaatcat atcgtcaaca taccggccgt
      781 aatgtttcat tccgaatttt tcgcgcacca gatgatccag ttcgtccaga tagatattgc
      841 cgaaaagctg gctggtcaga ttcccgatgg gaaagccttt tccgtttttc gcaaaaaaca
      901 gactttttga ttttggcagg ccgatccaat cctcccgctt gccttttatg tgaaagtttt
      961 tggtcggcgc atggaacacg atctcgcgaa ttaagtaaag cgccagatca acatcaaatt
     1021 ccgcgttttt ggccgctttc agtctttgct ctattttttg gtaaagaatg tttctgtcca
     1081 tcgccataaa atatcctttt atgtctaact tcaaaatcca gcagtctttt tggtaatttt
     1141 ccgagccgga gcggatgaaa tgatctgccc gccggattcc cattgccgtc cccttgccga
     1201 tccggcaact gtagctatct ttaatgaagt gtttttcaaa aatcgggttg atatagttga
     1261 aaatcaaatg gtgcacgacg cgatcgcgaa aatccgcggc gaatatctcc cgcttaaccg
     1321 gtttgttgac gataaagcaa atgcttcggc cgattttgta ttcgcggttt atgatttctt
     1381 cgtaaagatt gaataatttc ccctcgtaac cggcctcaaa agccagggcg ctggcggttg
     1441 atcgtttgtt tcttcgcgcg tcaaaatacg cgcggaacaa atcaagaagc aactgccggt
     1501 tgttttgttt tttgacaatc tctttttgca agtccttgac acagcggacc gagaagccgt
     1561 tggcccgatt attcgtgttc gccggattca ccgcggacga gttgaagttc aagttcgccg
     1621 cattcgtccc gctgggactg cccgaccagt agttgccgtt actgccccga ttgttaagcg
     1681 aggcgctggc gcgattgcgg ttgccggcta gcgggatgat aacctctgaa tttatcgcgc
     1741 catttttttg atgaagcggc gcaatatgcc cgaacgccgg gcaaaattcc ttgcttgcgg
     1801 caagggggtt attggatttg ctccgcacgc tcgcctgcgc gctggcaaca gacgattccg
     1861 gcggtttggt atttgacttg ctgtattttt cccagctggc aagttgtttg gaaacttctt
     1921 caatggcttg gtttatttca acgaattttt tgaggctcag ttggttgaaa tcctgcatca
     1981 ggcgcgtgag caagcggatt gtttccaaat tttctctcgc ttggctaagg tcggcaatct
     2041 tgtctgccgc tttgttggcg cgatagatat tttttatcag cgaggttcct tcgtttttca
     2101 tatcctgccc gaccgtgtat ctgtattcgc gcgcaaaacc gcttgaattc gcaaacagca
     2161 tcgtcaccaa tttgtaactc gctttatata ttggaagatt gtaatataga gccataatat
     2221 tttttttgaa a
//
LOCUS       DAOQWE010000133         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_55859_length_2218_cov_3.673601, whole genome shotgun sequence.
ACCESSION   DAOQWE010000133 DAOQWE010000000
VERSION     DAOQWE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55859_length_2218_cov_3.673601"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1882)
                     /locus_tag="PKK37_04010"
     CDS             complement(<1..>1882)
                     /locus_tag="PKK37_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81575.1"
                     /translation="LSGTVQTVAGIDLVGVSHADGAQSGALAFSTRNAGSWSERLRID
                     SSGNVGIGTATPGQKLDIQGNPANGEFIGSKILDTGLNRGLLMGVYKSTNGAYDLAGL
                     WPAAVTPNNTNYIFGTTGADADTYFGAPSGRNMHFQVAASTKMYIDSTGNVGIGTTGP
                     AVNLDVRSSATNVVAMSSVGNSDASHFLTYYGGTSGDNPAFFWKSGDSLRLGPASANN
                     GTGFAERLRIDSTGNVGIGTTSPAEALDVNGRVRLAQTTAPDTVADKLYNVSGNLLWN
                     GVNLTAAGALPVGTEGQMLYNNAGAWSAFDGLHWDDATSRLGIGTTSPIYKFNLAGAN
                     ASTDVGDASLLGIVNTDTTANNTIGLAFGQANLSGTVQTVAGIDLVGVSHADGAQSGA
                     LAFSTRNAGSWGERLRIDASGNVGIGTAAPNSYPDAPLSLLHVNGQNQVTDSWGTALL
                     TSNGAMGINKGGSLTMGGSYFGTTLAGFGAIAGRKENSTSGNPAGYLQLSYGNSSGSL
                     VEGMRIDSTGNVGIGTTSPGYKLTVQSEASETTLGDNLLTNGDFTNDFTGWTAGANWS
                     AATGAAVHTAGSVEAISQDITVTTGQYYQIDYTETGDTAGSLTFAIGSESVTRAYWEA
                     TSV"
     assembly_gap    1883..1982
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1983..>2218)
                     /locus_tag="PKK37_04015"
     CDS             complement(<1983..>2218)
                     /locus_tag="PKK37_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81576.1"
                     /translation="QTTAPATVTDKLYNVSGNLLWNGINLTSGGALPVGTEGQMLYNN
                     AGAWTAFNGLYWDDATSRLGIGTTSPIYKFNLAG"
BASE COUNT          480 a          670 c          501 g          467 t
ORIGIN      
        1 accgaagtgg cttcccaata tgctctcgtc acgctctccg acccaatagc aaaggtgagc
       61 gaacccgccg tgtcgccggt ttcggtgtaa tctatctgat agtattgccc ggtggtaacg
      121 gtgatgtcct gcgagatggc ctccaccgaa cccgcggtat gcaccgccgc gcccgttgcc
      181 gccgaccaat tcgccccggc agtccagccg gtgaagtcat tggtaaagtc cccattagtc
      241 aaaagattat cccccaaagt cgtctccgaa gcctccgact gcaccgtcaa cttatacccc
      301 ggactcgtcg tcccgatccc gacattgccg gtgctatcaa ttctcattcc ctccaccaaa
      361 cttccagaag aattgccata agataattgg agataacctg ccggattgcc ggatgtggag
      421 ttttccttgc ggcccgcaat cgcgccgaaa ccggccagag tagttccaaa ataagatcca
      481 cccatagtta acgaaccgcc tttattgata cccatggcgc cgttggaagt taacaacgcg
      541 gtcccccatg aatcggtaac ttggttttgg ccgttaacat gcaacaggct taggggtgcg
      601 tccggatagc tgttcggcgc ggccgtgcca atgcccacat tgccggaggc gtcaatgcgc
      661 aggcgttcgc cccagcttcc tgcatttctt gttgaaaacg ccagcgcgcc gctttgcgcg
      721 ccatccgcat ggctcacgcc caccagatca atcccggcca cggtttgcac cgtgccgctc
      781 aaattcgcct ggccgaaagc aaggccaatg gtgttgttgg cagtggtatc ggtattcacg
      841 atccccagca aactcgcatc gcccacatcc gtgctggcat tcgccccggc gagattaaac
      901 ttgtaaatcg gcgaagtcgt gccaatcccc aggcgcgaag tggcgtcgtc ccaatgcaga
      961 ccgtcaaagg ccgaccacgc gcccgcattg ttgtagagca tctgcccttc ggtgcccacc
     1021 ggcaaggcgc cggcggcggt gaggttaacc ccgttccaca gcaaattgcc cgacacattg
     1081 tagagcttat ctgccactgt gtccggcgcg gtagtctgcg ccaaccgcac ccgcccattg
     1141 acatccagcg cctccgccgg actggtcgtg ccgatcccga cgttgcctgt actatcaatc
     1201 cgtaaccttt cggcaaaacc agttccatta ttagcacttg cggggccgag cctaagagaa
     1261 tcgccacttt tccagaaaaa agccgggtta tcaccactcg taccgccata ataagttaag
     1321 aaatgtgacg catcggaatt accaaccgac gacatcgcca ctacatttgt agcactggaa
     1381 cgcacatcca aattcactgc tggtcccgtc gtcccgatgc cgacattgcc ggtactatca
     1441 atatacattt tggtggaagc ggcaacttga aaatgcatat ttctccctga tggcgcacca
     1501 aaataagtat ctgcatcagc tccagtggta ccaaaaatat aatttgtatt atttggcgta
     1561 accgctgcgg gccaaagtcc tgccaaatca taagcaccat tagtagattt ataaacaccc
     1621 atcaacaatc ctctattcaa ccccgtatct aaaatttttg aaccgataaa ttcaccattt
     1681 gcaggattac cttgtatatc caacttttgc cccggcgttg ccgtaccaat tcccacattg
     1741 ccggagctat caatgcgcaa acgctcgctc caactgcccg cgtttcttgt tgaaaacgcc
     1801 agcgcgccgc tctgcgcgcc atccgcgtgg ctcacgccca ccagatcaat cccggccacg
     1861 gtttgcaccg tgccgctcaa atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnccccggcg agattaaact tgtaaatcgg cgaagtcgtg ccaatcccca ggcgcgaggt
     2041 ggcgtcgtcc caatataatc cgttaaacgc agtccacgcg ccggcattgt tgtagagcat
     2101 ttgcccttcg gtgcccaccg gcagagcgcc gccggaggtg aggttaatcc cgttccacaa
     2161 cagattgccc gacacattgt agagcttgtc cgtcactgtc gccggcgcag tggtctgc
//
LOCUS       DAOQWE010000134         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_56422_length_2204_cov_6.757562, whole genome shotgun sequence.
ACCESSION   DAOQWE010000134 DAOQWE010000000
VERSION     DAOQWE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56422_length_2204_cov_6.757562"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PKK37_04020"
     CDS             complement(<1..1054)
                     /locus_tag="PKK37_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNR81577.1"
                     /translation="MDFEIFKTKIEGKDQKFNLTDAKERRDYFLAKAGEEIEKLKSYV
                     KDNSFIVYLLGKKNSGKGTYSKMLAELLGPGSLEHFSVGDMIRAVSRDIQDPAKKLEL
                     ELYLKKNYRGWTPLDDLIAAIEGRNTKTLLPTELILALVKREIAKRPHKTLFIDGFPR
                     DMDQVSYSLFFRDLVGFRDDADVMAFIDVPTAVIEERIKYRRICPICNTSRNTKLFPT
                     ARVDYDEKLEEFHLICDNPDCSGNGRQRMVGKEGDNLGIEPIRERLEIDQKLMEKAMT
                     LHGLPKIFLRNSVPVDAAKNYVDDYELTMAYDYEWDAAAKKINIIEKPWVVNDDQGVP
                     SYSLQAPAVAVSLIKQL"
     gene            complement(1182..2060)
                     /locus_tag="PKK37_04025"
     CDS             complement(1182..2060)
                     /locus_tag="PKK37_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNR81578.1"
                     /translation="MDGGEKKHRFAKWAILALAVADLFVWLALAESGRQNFTEVRFFD
                     VGQGDAAFVQTARGTQIIIDGGPGNSVLEKLGRAMPFYDRQIDWMILSHPDGDHLSGL
                     LAVLENYRVDNIVWSGIGKDSAECRQWEEMIAREGARIFEVSARDKFTLDGGAVVVEI
                     LSPETESAGAKESSNNLSVAARIIHKNRAFLFTGDIDAKKEELIFRANPDLRADVLKI
                     AHHGSKYSANEEFTAGSAVAAAVVSCGKNNRYGHPHPETLDLLEKYDIKTLRTDEDGN
                     IVFRTDGERIFVNTQK"
BASE COUNT          533 a          619 c          481 g          571 t
ORIGIN      
        1 ccaattgctt gatcaaggac acggccaccg ccggcgcttg caggctgtat gacggcacgc
       61 cttgatcgtc gttgaccacc cacggttttt caatgatatt gatctttttg gccgccgcgt
      121 cccactcata atcataggcc atcgtcaatt cgtaatcgtc aacataattt ttggccgcat
      181 caaccggcac ggaattgcgc aaaaatatct tgggaagtcc gtgcagtgtc attgcttttt
      241 ccatcaattt ttggtcaatc tccagccgct cgcggatcgg ctcaataccc aaattatccc
      301 cctccttacc caccattctc tgccggccgt tccccgaaca atccgggtta tcgcaaatca
      361 gatggaattc ttccaatttt tcgtcataat caacccgcgc cgtcggaaaa agcttagtat
      421 tgcgcgaggt attgcagatc ggacatatcc ggcggtattt gatgcgctct tcaatcacgg
      481 ccgtgggaac gtcaatgaac gccatcacat cggcgtcatc gcgaaaccca accagatcgc
      541 ggaagaacag cgagtaggac acctgatcca tatcccgcgg aaatccgtca ataaacagcg
      601 tcttgtgcgg ccgtttggca atctcgcgct tcaccagcgc caagattaac tcggtgggca
      661 aaagcgtttt ggtgttgcgg ccctcaatgg cggcgatcaa atcgtcaagc ggcgtccagc
      721 cgcgatagtt ttttttcaaa taaagctcca attccagctt tttggccgga tcctggatat
      781 cgcgcgacac cgcgcggatc atatcaccca cggaaaaatg ctccagcgat ccgggaccca
      841 gcaattcggc cagcatcttg gaataggtcc ccttgccgga attcttcttg cccaaaaggt
      901 agacaatgaa gctgttgtcc ttgacgtagc ttttgagttt ttcaatctcc tcccccgcct
      961 tggcaagaaa ataatcccgc cgctccttgg cgtcggtaag attgaatttt tgatccttgc
     1021 cttcaatttt ggttttaaaa atttcaaaat ccatattttt atttaaaatt taaaaatgaa
     1081 aagctaaaaa cgaaaactaa aagctaaaaa ccgagtgagt tgtatttttt aaatttttaa
     1141 ttttaaattt tcgtttttac tttttcgttc ttaattttta attatttttg tgtattcaca
     1201 aaaatgcgct ccccgtccgt gcgaaaaaca atgtttccgt cctcgtcggt gcgcaatgtt
     1261 ttaatatcat atttttccaa caaatccaag gtttccggat gcggatggcc gtagcggttg
     1321 ttctttccgc acgaaaccac cgcggcggcg accgccgatc cggccgtgaa ttcctcattg
     1381 gccgaatatt tactgccgtg atgggcgatt ttcaaaacat cggcccgcaa atcaggattg
     1441 gcccgaaaaa tcagttcctc cttcttcgcg tcaatgtcgc cggtgaaaag aaacgcgcgg
     1501 tttttgtgga tgattcttgc cgccaccgaa aggttgttgg aactttcttt cgcgccggca
     1561 gattcggttt cgggcgacag aatttccaca acgacggcgc cgccgtcaag agtgaactta
     1621 tcccgcgccg aaacttcaaa gatgcgggcg ccctcccggg caatcatctc ttcccattgc
     1681 cggcattcgg cggagtcttt gccgattccg gaccaaacga tattgtccac ccggtaattt
     1741 tcaagcaccg ccaggagtcc ggaaagatga tcgccgtcgg gatgcgacaa aatcatccag
     1801 tcaatttgcc ggtcatagaa cggcattgcg cgtcccagtt tttccagcac tgaattcccg
     1861 gggccgccgt caataatgat ttgcgtcccc cgcgcggttt gcacaaacgc cgcatcaccc
     1921 tgccccacat caaaaaaacg gacttcggta aaattttgcc ggccgctttc ggccagcgcc
     1981 agccaaacga aaagatcggc aacggccaac gccaaaatcg cccatttcgc gaaacgatgt
     2041 ttcttttcac cgccgtccat atcccgattt taacacaaaa gacgcggaac cgcgcccgcg
     2101 ccctgccaac aacttttaca aaactattct tccttgaaat ccttgggaag aattttatac
     2161 atcttcgtgc cgcacaccgc gcaatcgccg gtggccgcct tgcg
//
LOCUS       DAOQWE010000135         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_57392_length_2180_cov_6.948706, whole genome shotgun sequence.
ACCESSION   DAOQWE010000135 DAOQWE010000000
VERSION     DAOQWE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57392_length_2180_cov_6.948706"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..361
                     /locus_tag="PKK37_04030"
     CDS             80..361
                     /locus_tag="PKK37_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNR81579.1"
                     /translation="MIDAKTKKIIVEILKKHGVKRAAVFGSFARGEQKKRSDIDILVK
                     TPSNMSLLGFIGMENELKDALKRKIDLVEYGYVHPLLKKQIMSEQVPIL"
     gene            371..733
                     /locus_tag="PKK37_04035"
     CDS             371..733
                     /locus_tag="PKK37_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNR81580.1"
                     /translation="MKRTQKIYIYDIAECVEYVENYMKGVLEGNFLKDHQKQDAVFRR
                     LEIIGEAAKYISTDFWKKYPEVAWRDICGLRDKIVHEYFGIDPRKIWHIVKDDLPVLK
                     KQIAGILESMNNNQKKLL"
     gene            730..1872
                     /locus_tag="PKK37_04040"
     CDS             730..1872
                     /locus_tag="PKK37_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNR81581.1"
                     /translation="MNPKMYLKGVGMTKFDYAQRHWWQFAFDAALEALKNGKTNMGAI
                     DAIVFSGVSSAAGGEHQTHKVSFLSGLFKTNVPVIETPAVCGGGGLAFWTALRLLRDE
                     EFKNILVLAGEKLVDNRSEVTTDWILSAAERVMEQPEGLLFPAQNALVYQQYCQKYGD
                     NRETLELIAFKNHQNAEKNPKAYYYRRPVSLEKIKQSPEVASPLRLMDCSLSVNGGAA
                     AVVSKEESEIEIVGSGFATDYILTFERKDLTHFAATQIAAKKAFAMAGLSPQDINVAE
                     IHDAFTSVEMLSYEDLGFIGFGKAGELIRSGKINLDGEMPVNPSGGLKAKGHPISATG
                     LAQIYEIAEQLRGNCGERQAKNAKIGLTHNIGGAGGSVTVHIFRHT"
BASE COUNT          621 a          428 c          595 g          536 t
ORIGIN      
        1 cgggttcacg ataacataaa atcggggatt tgccaatttt ggcgtttttg gataggatgg
       61 gggcagacaa tatgccaaaa tgattgacgc caaaacaaaa aaaataatcg ttgaaattct
      121 caaaaaacac ggagtgaagc gcgccgcggt ttttgggtcg tttgcgcgcg gggagcagaa
      181 aaagagaagc gatattgata ttttggtcaa aacaccttcc aatatgagtc ttttggggtt
      241 tatcggaatg gaaaacgaat tgaaggatgc cttaaaaagg aaaattgatc tggtggaata
      301 tggatatgtg catcctttgc tgaaaaaaca aattatgtct gagcaagttc ctattttgta
      361 atttttaaaa atgaaaagaa cgcaaaaaat ttacatttat gatatcgccg agtgtgttga
      421 atacgttgag aattatatga aaggggttct tgaggggaat tttttaaaag atcatcaaaa
      481 acaagacgcg gtgtttcgaa gattggaaat tatcggtgaa gcggcgaaat atatttcaac
      541 ggatttctgg aaaaaatatc ccgaagttgc ttggagagat atttgcggat tgcgcgacaa
      601 aatagttcat gaatattttg gaattgaccc aagaaaaatt tggcacatag ttaaagatga
      661 tttgccggtt ttaaaaaagc agattgccgg gattttggag tcaatgaata ataatcagaa
      721 aaaactttta tgaatccgaa aatgtatctc aagggggttg gaatgaccaa gtttgattac
      781 gcgcaaaggc attggtggca gtttgcgttt gacgcggcgc tggaagcgtt aaaaaacggc
      841 aaaacgaata tgggcgcgat tgacgcaatc gtgttttccg gggtgtccag cgccgcgggc
      901 ggggagcatc agacgcacaa ggtttcgttc ttgtcgggat tgttcaaaac caatgttccc
      961 gtcattgaaa cgccggcggt ttgcggcggc ggcggattgg cgttttggac ggccttgcgg
     1021 cttctgcgcg acgaggagtt caaaaacatt ttggtgctgg ccggagaaaa attggtggac
     1081 aaccgttccg aggtgaccac cgattggatc ttgtcggcgg ccgagcgggt gatggaacag
     1141 cccgaaggat tgctgttccc ggcgcaaaac gcgctggtct accagcaata ttgccaaaaa
     1201 tacggcgaca accgggaaac tctggaattg atcgccttca aaaaccatca aaacgcggaa
     1261 aaaaatccca aggcctatta ctaccggcgg ccggtgtcgt tggaaaagat caagcagtcg
     1321 ccggaagtgg cgtcgccctt gcgtctgatg gattgctcgc tttccgttaa cggcggcgcg
     1381 gcggcggttg tttccaaaga agagagcgaa attgaaattg tcggctcggg atttgccacc
     1441 gattatatct tgacttttga aaggaaggat ttgacgcatt ttgccgccac gcaaattgcg
     1501 gcgaaaaaag cgtttgcgat ggcgggattg tcgccgcaag acatcaatgt cgccgaaatt
     1561 cacgacgcgt tcacttcggt ggagatgctg tcatatgaag atttgggatt tatcggattc
     1621 ggcaaagcag gggagctgat ccgttcgggc aaaatcaatc tggacggaga aatgcccgtc
     1681 aatccttccg gcgggctgaa agccaagggc catccgatct cggccacggg tctggcgcaa
     1741 atctatgaaa tcgccgagca gttgcgcggc aattgcggcg agcgtcaagc caaaaacgcg
     1801 aaaatcggcc tgacgcacaa catcggcggg gcaggcggga gcgtaacggt gcatatcttt
     1861 cgccatactt gatatatcgg ccgccttcgc caaggctacg gcgagctaaa agcggcaccg
     1921 gcggttcggt taccgtgcat atttttaaaa gatcgtagtt tctattgaca ctatataact
     1981 attgtatata ctatagttat taggttaaac tattcaaaac tatgaaaact ttgatcaata
     2041 tcaaaaccga ccgcgaagtg aaaatcagcg cccaaaaact ggccaaagaa ttggggttgt
     2101 ctttgagtgc ggtggtcaat gcttatttga agcagttcgt gcggtcgcaa gccgtggatt
     2161 tttcggttgc gccgcaaatg
//
LOCUS       DAOQWE010000136         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_60406_length_2112_cov_9.280019, whole genome shotgun sequence.
ACCESSION   DAOQWE010000136 DAOQWE010000000
VERSION     DAOQWE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60406_length_2112_cov_9.280019"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKK37_04045"
     CDS             <1..699
                     /locus_tag="PKK37_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNR81582.1"
                     /translation="GKNSIEELAAAAMAMGYEYLGVADHTRALKVAGGLDEDRLRQRN
                     AEIDALNEKIRSQGKIFAVLKGCEANIDDDGGIDLDKEILAELDFVIAGVHMNFKMDA
                     KKMTRRLVGAAKNPDIDIISHPTGRLLKERPAYELEIEKVFEVAKEHGAILEINAQPR
                     RLDLKPLHVRGAIANGIKLAINTDAHRPAELEFARFGIAQARRGWATAGDIANTLPWP
                     ELKKILKRNQKRVN"
     gene            750..1196
                     /locus_tag="PKK37_04050"
     CDS             750..1196
                     /locus_tag="PKK37_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR81583.1"
                     /translation="MKFEKSAGAIVFRRGRGGETYFLLLHYPSATHRSHKDYWDFVKG
                     HVEKGEGEIATVRREAAEETGLTDLEFTAGFRVPMRYFFRHRGGLIFKTAVFYLAETK
                     NENITLSGEHDDFIWLSHPKAREMLSFKNAKKIFGEAGKFLNIAGK"
     gene            1193..>2112
                     /locus_tag="PKK37_04055"
     CDS             1193..>2112
                     /locus_tag="PKK37_04055"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HNR81584.1"
                     /translation="MSRRRKQKTAALVLFSGGLDSILAVKILRAQDIDATPVCFESYF
                     FSADKAQSAAAASGLDLRVEDISKSHLSIVKNPCHGRGAAMNPCIDCHLLMIKIAGGI
                     MAREGFDFIATGEVLGQRPMSQHRQSLAIIDRQSGLAGKILRPLSAKLLAPTEAEING
                     LVDRDGLYDLSGRSRRGQLALAKKFGIKNIPQPGGGCILTDAEYARRLEELMRRQPDF
                     DGDDARLLRHGRAVWQKDLLAVIARNQAECQNLKIMARAGDWLFEPQNFPGPTVLVRS
                     FGKKNGQGETLPDSGEIKKMGQDFVRRYSK"
BASE COUNT          535 a          489 c          604 g          484 t
ORIGIN      
        1 ggcaaaaatt caattgagga attggcggcc gcggccatgg cgatgggtta tgaatatttg
       61 ggcgttgccg atcatacgcg tgcgttgaaa gtggccggcg ggttggatga agatcgattg
      121 cgccaacgca atgccgaaat tgacgcgctc aacgaaaaaa taagatccca aggcaaaata
      181 tttgccgttc tcaaagggtg cgaggccaat attgacgatg acggcgggat tgatttggac
      241 aaagaaattt tggccgaatt ggactttgtg attgccggcg tgcatatgaa ttttaagatg
      301 gacgccaaaa aaatgacgcg ccgcttggtt ggtgcggcca aaaatcccga tattgacatc
      361 atttcgcatc ccaccggccg tttgctcaaa gagcggccgg cttatgaatt ggaaattgaa
      421 aaagtgtttg aggttgccaa agagcatggc gcgattttgg aaatcaacgc ccagccccgg
      481 cgcttggatt taaagccgtt gcacgttcgc ggggcgatcg ccaacggcat taaactggcg
      541 atcaataccg acgcgcaccg tccggccgaa ttggagttcg cgcgattcgg cattgcgcag
      601 gcgcggcgcg gctgggcgac ggctggcgat atcgccaaca ccttgccgtg gccggaattg
      661 aaaaaaatct tgaaaagaaa ccaaaaaaga gtaaattaaa gaaggattgt tccatcatta
      721 accttgcaat ctaaaccaat caaaaccata tgaaatttga aaaatccgcg ggcgcgatcg
      781 tttttcgccg cggccgcggc ggagaaactt attttctgtt gttgcattat ccttccgcga
      841 cgcatcggtc gcacaaagac tattgggatt tcgtcaaggg gcacgtggaa aaaggagagg
      901 gggaaattgc caccgtgcgc cgcgaagccg ccgaagaaac cggtctgacc gatctggaat
      961 ttactgccgg ctttcgcgtc ccgatgagat attttttccg gcaccgcggc ggcttgatat
     1021 tcaaaacggc ggttttttat ctggcggaaa caaaaaacga gaatatcact ttgtcggggg
     1081 aacacgatga tttcatttgg ctgtcccatc ccaaggcgcg ggaaatgctt tcgttcaaaa
     1141 atgcgaaaaa aatttttggg gaagccggca aatttttaaa cattgcggga aaatgagtcg
     1201 ccggcgcaaa caaaaaaccg ccgcgcttgt tttgttttcc gggggattgg attcaatttt
     1261 ggcggtgaaa attttgcggg cgcaagacat tgacgcgacg cccgtttgtt ttgagagtta
     1321 ttttttttcc gccgacaagg cgcaatccgc ggccgccgcg tccggattgg atttgcgggt
     1381 ggaagatatt tcaaagtccc atctgtcaat tgtgaaaaat ccgtgccatg gccgcggtgc
     1441 ggcaatgaat ccctgcattg actgccatct gttgatgatt aaaatcgccg gcggaataat
     1501 ggcgcgggaa ggatttgatt ttattgccac cggcgaagtg ttgggacagc ggccgatgtc
     1561 gcaacaccgc caatcgctgg caatcattga ccggcagagc ggtctggccg gtaaaattct
     1621 ccggccgctg tcggcgaaat tgttggcgcc gaccgaagcg gagatcaacg gattggttga
     1681 tcgggatgga ttatatgacc tatccgggcg gtcgcgccgg ggccagctgg cactggcaaa
     1741 aaaattcggt atcaaaaata ttcctcagcc gggcggcgga tgcattttga ccgatgccga
     1801 atatgcccga cggctggaag aactgatgcg ccgccagccg gattttgacg gcgatgacgc
     1861 gcggcttttg cgtcatggcc gcgcggtttg gcaaaaggat ctgctggcgg tgattgcccg
     1921 caatcaggcg gaatgccaaa atctcaaaat tatggcgcgt gccggcgatt ggctttttga
     1981 gccgcaaaat tttcccggcc ccactgttct tgtccgcagt tttggcaaaa aaaatggtca
     2041 aggtgaaacc ttgcccgact ccggcgagat aaaaaagatg ggacaagatt ttgtccggcg
     2101 atattcaaaa aa
//
LOCUS       DAOQWE010000137         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_60923_length_2101_cov_4.785435, whole genome shotgun sequence.
ACCESSION   DAOQWE010000137 DAOQWE010000000
VERSION     DAOQWE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60923_length_2101_cov_4.785435"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PKK37_04060"
     CDS             <1..565
                     /locus_tag="PKK37_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNR81585.1"
                     /translation="ITETDLESYCQPDSKFIGLAEPIIPDIPAAGGSMGFGLPFGVGF
                     ALAKKTKKEEGKIFVVMSDGEMQIGTTWESAMIAAHHKLDNLLVFVDCNGLQAMGNVG
                     EILKVEPLAEKWKAFGWNVCEIDGHDFDEIEKIAGSPVFGKPTAVIARTIKGKGVSFM
                     EGQNLYHYKAPSEEEYQAALNELKING"
     gene            558..1064
                     /locus_tag="PKK37_04065"
     CDS             558..1064
                     /locus_tag="PKK37_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81586.1"
                     /translation="MAEIIQQRDTRREFIDTLIDLAQKDEKIVLVIPDVGFNYIEEFQ
                     KRFPDRFFNLGVTEQSAMTIAAGMALEGLKPYIYSMINFVAFRPYEAVRNAVCLHRAN
                     VKIMGVKGSEKYKFLGFSHNLIVDDEEFRIFSHLPDFKTYLAQKPEDVKSIIMESYAS
                     PNPCYIRL"
     gene            1215..>2101
                     /locus_tag="PKK37_04070"
     CDS             1215..>2101
                     /locus_tag="PKK37_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81587.1"
                     /translation="MIIQREIQPQVEDALFKGKIVVIYGPRQVGKTTLAKLILQNRQE
                     IKSAYFNCDEPDICQALTDKTSTELKLFLGDSKLVVLDEAQRVKNIGLTLKLLADNYP
                     KMQIIATGSSSFDLSNQIFEPLTGRKKEFYLYPFSLAELKNYYSEIEFNRILENRMVF
                     GMYPGVIFESDKDKSESLRELAKSYVYKDALEYQGIKNSEVVEKLLQALALQVGNEVS
                     YNEISSLIEVNKITVANYIQILEKAFIVFRLPSFSRNLRNEIKSNRKIYFFDNGIRNA
                     LINNLNPVDLRQDAGALWE"
BASE COUNT          638 a          382 c          511 g          570 t
ORIGIN      
        1 gatcaccgaa acggatttgg aaagttattg ccagccggac agtaagttta tcgggctggc
       61 cgagccgata attcccgata ttcccgcggc cggtggctca atgggatttg gtctgccgtt
      121 tggagtcggg tttgcgttgg ccaaaaaaac caagaaggaa gaggggaaaa tttttgtggt
      181 aatgtcggac ggcgaaatgc aaatcggcac cacctgggaa tccgcaatga ttgccgctca
      241 tcacaaattg gacaatcttt tggtgttcgt ggactgcaat ggtttgcaag cgatggggaa
      301 tgtcggcgag attctgaagg tggagccgct ggcggaaaaa tggaaggcgt tcggctggaa
      361 tgtttgcgaa attgacggcc atgattttga tgaaatagaa aaaattgccg gcagtccggt
      421 ttttggcaag ccgacggcgg tgattgccag aacgatcaag ggcaagggag tgagctttat
      481 ggaggggcaa aatttatatc attacaaagc accgtccgag gaggaatacc aagcggcgct
      541 taatgaatta aaaatcaatg gctgaaataa tccaacaacg agacacgcgc cgggaattta
      601 ttgacacact gattgatttg gcccaaaagg atgaaaaaat cgtcttggtg attcccgatg
      661 tgggttttaa ctatattgaa gaatttcaaa aaagatttcc cgatcggttt ttcaatttgg
      721 gcgttaccga gcaaagcgcg atgacgattg ccgcggggat ggcgttggag ggtttgaaac
      781 cctatattta cagtatgatc aactttgtcg ctttccggcc ctatgaagcg gtgcgcaacg
      841 cggtgtgctt gcatcgcgcc aatgtgaaga ttatgggcgt gaaggggagc gaaaaataca
      901 aatttttggg atttagccac aacctcatcg ttgatgacga agaatttcgg atattcagcc
      961 atttacccga ttttaaaact tatctggccc aaaaacccga agacgtgaaa agtatcatca
     1021 tggaatccta cgcctcgcca aatccctgct atatcaggtt ataaggagaa gccatgaatt
     1081 ggatcataat tttcgatagc cggattatgt ataaatttgg acattaaaat ggccaaatta
     1141 gtatataaat cggttagggg attgacaaat gcccaaatta atatataatt tagtcagtgt
     1201 aatgacgaaa ttatatgata attcaaagag aaatccaacc gcaagtggag gatgcccttt
     1261 ttaaaggtaa aattgttgtg atctatgggc cgcggcaagt gggcaaaacc actttggcca
     1321 aacttatttt gcaaaaccgg caagaaataa aaagcgcgta tttcaattgc gacgagccgg
     1381 atatttgtca agcgttaacc gataaaacct caaccgaatt gaaattattt ttaggcgaca
     1441 gcaagctcgt ggttttggac gaagcccaac gggtgaaaaa tatcggtttg actttaaaac
     1501 ttttggctga taattatccg aaaatgcaaa ttattgccac cggctcatcg tcgtttgatt
     1561 tgtcaaacca aatttttgag cccttgaccg gccgcaaaaa agaattttat ttatatcctt
     1621 tttcgctggc ggaattgaaa aattattatt cggaaattga attcaatcga atcttggaaa
     1681 acagaatggt ttttggtatg tatccggggg ttatttttga aagcgataaa gataaatccg
     1741 aatcgttgcg ggagttggcg aaaagttatg tttacaaaga cgcgctagaa tatcaaggta
     1801 ttaaaaattc ggaagtcgtg gaaaaattat tgcaagcgct ggcgcttcag gttggcaatg
     1861 aggtttcata taacgaaatc tcgtcattga ttgaagtcaa taaaataacc gtggccaatt
     1921 atattcaaat tcttgaaaag gcctttattg tttttcgttt gccttctttt tccaggaatt
     1981 tgcgcaacga aatcaaaagc aatcgcaaaa tttatttttt tgacaacggc atccgaaacg
     2041 cgttgatcaa caatttaaat cccgttgatt tgcgccaaga cgcgggcgcg ctttgggaaa
     2101 a
//
LOCUS       DAOQWE010000138         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_63921_length_2039_cov_3.500504, whole genome shotgun sequence.
ACCESSION   DAOQWE010000138 DAOQWE010000000
VERSION     DAOQWE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63921_length_2039_cov_3.500504"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..350)
                     /locus_tag="PKK37_04075"
     CDS             complement(63..350)
                     /locus_tag="PKK37_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81588.1"
                     /translation="MEKKGFWQVVWYWYYKNWQWIFKILFWVIMLVAFAILLSVPQDK
                     YNIYVGFTIVGSAVIFVMAIGCIMSWLAERYLRNYDADYRWHLKYNRGERL"
     gene            complement(517..1278)
                     /locus_tag="PKK37_04080"
     CDS             complement(517..1278)
                     /locus_tag="PKK37_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81589.1"
                     /translation="MVNTTVFAPRFSITTENFGFAWLKALSNALKGKDIVFGSQKEPK
                     YARDTVHEIVLTGGAIEEILNGELHPKFPFKSIDQYKREFQPDFLAEYDEWPPEKKFT
                     YLYVDRILRWKSPELKVAVNQLEDLRLQLEAQISSNIASNRSQVITWVPPIDRVSDAS
                     PCLQRIWQRPYFDKEEDVWLIDVQNDWRSRDLFNAWQPNLIALIGMLDEQIYEPLGCR
                     IGRVIDKINSLHIYRGDIEIAEQIVKEYLPPSMRI"
     gene            1368..2036
                     /locus_tag="PKK37_04085"
     CDS             1368..2036
                     /locus_tag="PKK37_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNR81590.1"
                     /translation="MKIAIVGAGVCGLYLAKNLAQRKNEVFVFEKKKTIGKQCCSGLF
                     SARLFDFFPEARQLETNQIGSCIINFPKRTIRIKFRPRFSVIDHAQLDLLAGALATRA
                     GANIRTNQNIDFAKLDELSAEYDRLIGCDGALSPTRNYLTFKKGPTLLKKGSDPFCLS
                     FPLGIQGFEQKRDNSDFVETWATKNGFLWRIPRGKDVEWGIMEKPDWRRKFLMNLSHE
                     KISN"
BASE COUNT          626 a          441 c          368 g          604 t
ORIGIN      
        1 aaagttataa gatttacacg caaataatag cgcaaatctt gccttttgtc aaaaaaagag
       61 ggctacagcc tttcgccacg attatattta agatgccatc gataatcagc gtcataattg
      121 cgcaagtagc gctccgccaa ccacgacata atacatccaa tcgccattac gaaaataact
      181 gctgacccaa caatagtaaa cccaacatag atattatatt tatcttgcgg cacagaaagc
      241 agaatagcaa acgccacaag cattattacc caaaataaaa ttttaaatat ccattgccag
      301 tttttataat accaatacca taccacttgc caaaatcctt tcttttccat taaaatcaca
      361 ctccttgtta aatgactatt aacacgatgg tatcaaaata taaaataaag tcaataaaat
      421 ttgaagatcg cgatcatcat ggccggggtc tgcgggctac cgttattgtc aaacccttgc
      481 cttttgtcaa aaaaaataag aatggcattc cattctttat atccgcatag acggaggtag
      541 atattctttt actatctgtt cggctatttc tatatctcct cgatatatgt gcaaagagtt
      601 gattttgtca atcacgcgcc caatacgaca accaagcggt tcataaattt gctcatcaag
      661 catcccgata agcgcaatta aatttggttg ccatgcgtta aaaagatcac gcgacctcca
      721 gtcattttgc acgtcgatta gccaaacatc ctcttcctta tcaaaatatg gtctttgcca
      781 tattctttgc aagcaaggag aagcatcgct taccctgtct atcggcggaa cccatgttat
      841 gacttgcgat ctattggaag caatatttga tgaaatttgc gcttccaatt gcaatcgcaa
      901 atcctcaagt tggtttacag ctacttttaa ttccggagat ttccatctca aaatacgatc
      961 aacataaaga taagtaaatt ttttttccgg tggccattca tcgtattccg ccaaaaaatc
     1021 cggctgaaac tctcgtttat attgatcgat agatttgaaa ggaaacttcg gatgcaattc
     1081 tccattcaat atttcttcaa tcgctccacc agttaaaaca atttcatgca cggtatctct
     1141 tgcgtatttt ggctcttttt gagagccaaa aacaatatcc tttcctttca aagcattact
     1201 tagcgctttc agccacgcaa aaccaaaatt ttccgttgtg attgaaaatc ttggcgcaaa
     1261 cactgtcgta ttaaccacct ctttagtttt taatcaagtt tttaatcttg ctagctaaaa
     1321 tgatactaac aaagtataat gttaaattca atagttttta tttttttatg aaaattgcga
     1381 ttgtcggggc cggggtctgc gggctctatt tggcaaaaaa tttggcgcaa aggaagaatg
     1441 aggtttttgt ttttgaaaag aaaaaaacca tcggcaagca atgctgttcg gggctgtttt
     1501 cggcgcggct ttttgatttt tttcccgaag cgcgacaact tgaaaccaat caaatcggtt
     1561 cttgcatcat caattttccc aaaagaacga tacggataaa atttcgcccg cgattttccg
     1621 tcattgatca cgcgcagttg gatttgcttg ccggcgcttt ggcaacgcga gcgggagcca
     1681 acatccgcac caatcaaaac attgattttg ccaaattgga cgagttatcc gccgaatatg
     1741 atcgcctcat cggttgcgac ggcgccttgt cgcccacgcg aaactatctt acttttaaaa
     1801 agggtccgac ccttttaaaa aaagggtccg accctttttg tttgtcattc ccactgggaa
     1861 tacagggatt tgaacaaaag cgggataatt cggattttgt ggagacttgg gcgacaaaaa
     1921 acggatttct gtggcgaatc ccgcgcggca aagatgttga atgggggata atggaaaaac
     1981 ctgattggcg ccgaaaattt ttaatgaatt tatcgcacga aaaaatatca aattaacgg
//
LOCUS       DAOQWE010000139         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_65388_length_2011_cov_3.038855, whole genome shotgun sequence.
ACCESSION   DAOQWE010000139 DAOQWE010000000
VERSION     DAOQWE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65388_length_2011_cov_3.038855"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PKK37_04090"
     CDS             complement(<1..1077)
                     /locus_tag="PKK37_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81591.1"
                     /translation="MAFQLKKYQQAALERLRDYLLEMKKYQTDKTAGVAFMTLRTDAA
                     DGFDKNYHWIPELGHSPFVCVKIPTGGGKTLIAAHALGAIFADYLGQRNDKGLAMWFV
                     PSDAIKGQTLINLRDRNHPYRETLDRRFGNAVKIFDLAEAKAVKPDDLANNLCVVIST
                     LSAFRRTNREWLKVYQDNGALLPHFEEVNGVDFDFLEKDSQGEIIYSLANVIKLCHPP
                     VIVDEGHNAQTNLSFEMLKTLNPSFILEFTATPRSQSNVLVNVSAKELKAEKMIKMPI
                     YLANKTPWQETICDGIAKLAELEKIAKKESGGHIRPIMLLQAEQEKESKTKIFVDQIY
                     KFLTEERKIPAEQIAIQTGSKKDLP"
     gene            complement(1079..1873)
                     /locus_tag="PKK37_04095"
     CDS             complement(1079..1873)
                     /locus_tag="PKK37_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR81592.1"
                     /translation="MRRVKRNGGFVYDVFFKKYLKDELKSKLSNIWAESDFRTEIGTK
                     ELKEIFNGKKIFAYPKPMVTIRQVIQSCTLNNDVILDSFAGSGTTGHAVLDLNKEDGG
                     NRKFILVEMEKEIAQKITAERVRRVARGYAHKNAKGETIKIAGLGGGFEYVQLGAPLF
                     DKNGAINPEVGFEDMARYIFFTETRTNLEKKNIAGNFIGEFGGGKYYLLFDGIGKNIL
                     DMRFAQKLKKIDGQKIVYADKCLVSDEFLEECGAVFKQIPYEIKIY"
BASE COUNT          516 a          513 c          348 g          634 t
ORIGIN      
        1 cggcagatct tttttgcttc cggtttgaat cgcgatttgc tcggcgggaa tttttctttc
       61 ttcggtcaaa aatttataaa tttgatcaac aaaaattttt gttttgcttt ctttttcctg
      121 ctcggcttgc aaaagcataa tcggacgaat gtggccgccg ctttcttttt tggcaatctt
      181 ttccaattcc gccaatttgg cgattccgtc gcaaatcgtt tcctgccaag gagttttgtt
      241 tgccaaataa atcggcatct taatcatttt ttctgctttc aattcctttg ccgaaacatt
      301 gaccagcaca ttgctttgac ttcgcggcgt ggcggtaaat tccaaaataa aacttggatt
      361 aagagttttc agcatttcaa aagacagatt ggtttgcgcg ttatggcctt cgtcaacaat
      421 aaccggcgga tggcaaagtt taatcacatt tgccagcgaa taaataattt cgccctggct
      481 atctttttcc aaaaaatcaa aatcaacacc gttaacttcc tcaaaatggg gcaaaagcgc
      541 cccgttgtct tgataaacct tcagccattc tcgattggtg cgccgaaacg cgctcaatgt
      601 tgaaatcacg acgcaaagat tgttcgccag atcatcgggc ttgacggctt tggcttcagc
      661 caagtcaaaa atcttcaccg cgttgccaaa ccgccggtca agcgtttctc gatagggatg
      721 gttgcggtcg cgcaaattta tcaatgtttg acctttgatg gcatccgacg gcacaaacca
      781 catcgccaaa cccttgtcgt ttctttgccc caaatagtcg gcaaaaatcg cgcccagcgc
      841 gtgcgccgca atcaaagttt tgccgccgcc ggtggggatt ttcacgcaca caaacggact
      901 gtgccccaat tccggaatcc aatgataatt tttatcaaac ccgtcggcgg catcggtgcg
      961 caaagtcata aatgccacgc cggcggtttt atcggtttga tattttttca tttccaacaa
     1021 ataatcccgc aatcgctcca gcgccgcttg ctggtatttt tttaattgaa acgccatatc
     1081 aatagatttt tatttcatag ggaatttgct tgaaaaccgc gccgcactcc tccaaaaatt
     1141 catcgctcac caaacacttg tcggcataga cgattttttg accgtcaatc ttttttaatt
     1201 tttgcgcgaa cctcatatcc aaaatatttt tgccaatgcc gtcaaacaaa agataatatt
     1261 tcccgccgcc gaactccccg ataaaattgc cggcgatatt tttcttttcc aaattggtgc
     1321 gcgtttcggt gaaaaatatg tagcgcgcca tatcctcaaa accgacctcg ggattgatcg
     1381 cgccgttctt gtcaaaaagc ggcgcgccca actgcacata ttcaaaaccg ccgcccaaac
     1441 cggcgatttt tatcgtttcg ccttttgcgt ttttatgcgc atagccccgc gccacccgcc
     1501 gcacccgctc agccgtaatt ttttgcgcga tttccttttc catctccacc aaaataaatt
     1561 tccgatttcc gccatcctct ttgttcaaat ccaaaaccgc gtgccccgtc gtccccgacc
     1621 ccgcaaacga atcaagaata acatcgttat tcaaagtaca agattgtata acttgcctaa
     1681 tcgtcaccat tggctttgga taagcaaaaa tctttttacc attaaaaatt tccttaagtt
     1741 cttttgtgcc aatttctgtt ctgaaatcac tctctgccca tatatttgac aattttgatt
     1801 ttaattcatc ttttaaatat tttttaaaaa aaacatcata aacgaaacca ccgtttcttt
     1861 taacccttct cacttcaatt aaatttttgt cattttcaat ttttggtttt ccccatatcc
     1921 accgaccatc gccaccctct ggaagtatcg gaaaaatttc aatatcataa ttaatttttt
     1981 ttacagttga tatcttgttt tgtttttcgt t
//
LOCUS       DAOQWE010000140         1997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_66123_length_1997_cov_5.123069, whole genome shotgun sequence.
ACCESSION   DAOQWE010000140 DAOQWE010000000
VERSION     DAOQWE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66123_length_1997_cov_5.123069"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKK37_04100"
     CDS             <1..432
                     /locus_tag="PKK37_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81593.1"
                     /translation="RNEAIDKELFIKIKNEILPSEIIRISMKEHDFGDKFLCENHDVL
                     RKFNDDYGKRPDFEFFDPELEKLLIELKNKIANFIALSGRYLFVIDKNYYGFRREFRD
                     NFPERYGKARKSLNESASAIWEIYETFIKLSRKKLLVSFEE"
     gene            585..>1997
                     /locus_tag="PKK37_04105"
     CDS             585..>1997
                     /locus_tag="PKK37_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81594.1"
                     /translation="MAKNKRKNGILLDASIIARTLAAYDPWWSEAGFAWNNPAFKRDV
                     FLQAISHIESGHGMALLINGPRRVGKTTIMRQLIEHLLKVKKIAPAEILFFSLDDPLV
                     QQLPQENQGEVFERIMEEWEAKLGKKIAQLPLPVYCFLDEVQRLPHWELYIKRYVDLK
                     YPIRFVISGSASHTIFRKSLESLLGRLVDISLPPFSFREWIRFHCGKYAVVCDELAGA
                     KIDIADKKIFSRTILEITKKLGAEKTKKLNELAGDYARDGGFPQLWEMKDLVERAQFI
                     DLQFVKRVTLEDLRLVREVRRPEIFHQFLRYVFARTGEEYNLEELAGKIHTTRPTLTD
                     ALPLLLQTELIRKVERFANKPVRLRSTHAKLYAADTVLYEAITKMPADLNGEDKGRLA
                     ETLAFNMLWRLPGISDICYFRTPDGKHEVDFILKIGKLFIPVEIKSGRVNINESEHIR
                     FFLKEYGAKESFGIMAHMGGI"
BASE COUNT          630 a          333 c          488 g          546 t
ORIGIN      
        1 agaaatgaag cgattgataa agaattattt atcaaaataa aaaatgaaat tttgccaagt
       61 gaaattataa gaatatcaat gaaagagcat gattttggtg ataaatttct ttgcgaaaat
      121 cacgatgtat tgcggaaatt taacgacgat tacggcaaaa gaccggattt cgaatttttt
      181 gatcctgaat tagaaaaatt attgatagaa ttaaaaaata aaatagctaa ttttatagct
      241 ttaagcgggc gatatttatt cgtgattgat aaaaattatt atggttttcg aagagaattt
      301 agggacaact tcccagagag atacggcaaa gcaagaaaaa gtttgaatga atcagcttct
      361 gcaatttggg aaatatacga aacttttatt aagttgagca gaaagaaatt gttggtcagt
      421 ttcgaggaat aagtatcgct tccgacaaaa tggaaacaaa tccctcaacg caagtttata
      481 aattggtgga gcgattgaat aagctgaaaa tcagttgaac aggggctttt atttgtttaa
      541 aattttatgt atagtatagt atacataaaa taaaattggt ttttatggcg aaaaataagc
      601 gcaaaaacgg aattttgctt gacgcaagta taattgcgag aaccttggcc gcttatgatc
      661 cttggtggag cgaggccggt tttgcgtgga acaatccggc gttcaagcgg gatgtttttt
      721 tgcaggcgat cagccacatt gaatccgggc atggaatggc gttgctcatc aacggtccgc
      781 ggcgcgtggg caaaaccacg attatgcgcc aactgattga gcatttgtta aaagtcaaaa
      841 aaatcgctcc tgccgaaatt ttgttttttt cgctggacga tccgcttgtc cagcaacttc
      901 cccaggaaaa ccaaggcgaa gtgtttgagc ggataatgga ggaatgggaa gccaagctgg
      961 gcaaaaaaat cgcgcaatta ccgttgccgg tgtattgttt tttggatgag gtgcagagat
     1021 tgccgcactg ggagctttat atcaagagat atgttgattt aaaatatccg attcgttttg
     1081 tgatttccgg ttcggcctcg cacacgatat ttcgcaaatc gctggaaagt ttgttggggc
     1141 gattggttga tatttcttta ccgccgtttt catttcggga atggatcaga ttccattgcg
     1201 ggaaatacgc cgttgtttgc gatgaattgg ccggcgcgaa aattgatatc gcggacaaaa
     1261 aaatcttttc acggacaatt ttggagatca cgaaaaagct tggcgcggaa aaaacaaaaa
     1321 aattgaatga attggccggc gattacgccc gcgatggagg gtttccccag ttgtgggaaa
     1381 tgaaagattt ggtggagcgg gcacagttca tagatttgca attcgtgaaa agagtgacat
     1441 tggaggattt gagattggtt cgcgaggtgc gccgaccgga aattttccac caattccttc
     1501 gttatgtgtt cgctcgcacc ggcgaagaat acaatttgga agaattggcc ggaaaaatac
     1561 ataccacaag accaacccta accgatgcgc tgccgttgct tctgcaaacg gaattgataa
     1621 gaaaagtgga aagattcgcc aataagccgg tgcgcttgcg aagcactcat gccaagttat
     1681 atgccgcgga tacggttctt tatgaggcga taaccaaaat gcccgccgat ttgaacggcg
     1741 aagacaaagg aagattggcc gaaaccttgg cgtttaatat gttgtggcga cttccgggaa
     1801 tatcggatat ttgttatttc agaacgcccg atggcaaaca cgaagttgat tttattttaa
     1861 aaatcggaaa attgttcatt cccgttgaaa taaaaagcgg gcgggtaaat ataaacgaaa
     1921 gcgagcatat aagatttttc ttgaaagagt atggcgcaaa agaaagtttc ggaataatgg
     1981 cgcacatggg cggaatt
//
LOCUS       DAOQWE010000141         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_67011_length_1981_cov_6.722741, whole genome shotgun sequence.
ACCESSION   DAOQWE010000141 DAOQWE010000000
VERSION     DAOQWE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67011_length_1981_cov_6.722741"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..>1981
                     /locus_tag="PKK37_04110"
     CDS             318..>1981
                     /locus_tag="PKK37_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR81595.1"
                     /translation="MNKLLKNKKGEKVMFLGNEAIIRGALESGVQFVSTYPGTPASEI
                     GNNFFALQKELKDDPKFLFEFSTNEKTAMEAAIGANFSGLKTLVAMKNFGLNVALEPL
                     LPLAYTGTRAPMVIFVADDPGCHSSAQSEENTRPFSVLGHLPMLEPSDAQECKDFTIE
                     AFAISEKFGTPVIVRITTRTAHQRALVALGELAPDRKVNLKGEFIKDKRKYVTMMPRL
                     HGMKKEMLAREEKLAQYFEKSKLNRVEGVIKGKNQPLGIITSGIAYLHAQEAMEELGV
                     GLPVLKLGMFHPLPEKKIRDFIKGLKKVLVLEEMEPYLEKRIQRLAKDANCKLEIRGV
                     DWRPQVGEMKPEIAAEMIAKFTGKKYTLPKFAPKFELPNRPPQLCPGCPYWLAFAAVK
                     KAVDANKVVFGGDIGCYMIGALPPHELYDYMFCMGSSIGIAHGIKKAAADQKVIAFIG
                     DATFFHSGIEGLLNAVYNGSNPLIIIMNNDITAMTGHQPHPDSVGAVHPIPIENMVLA
                     CGVKPENMKIIDQGQSEEFIAAVREFVDKPEVSVIIARRPCKFVEK"
BASE COUNT          531 a          443 c          539 g          468 t
ORIGIN      
        1 gcgacattgc cgatgacgtg atttatatcc cgaaaacttt ggaaatgctc acgccgattc
       61 tttccgcgat cacaatgcag ttattctctt attatgtggc attggcgcgg ggttgcgaca
      121 ttgacaagcc gcgcaacttg gcaaaatccg ttacggtgga gtgataaaaa ttaaaaatta
      181 aaaaatcaaa atggaaaatg gcagttcaaa atgttaaaat tggattgcct tttagtttat
      241 gaaagtaggc gggggttggc aaatactaaa gtatatttta gtatttgttt atgaaacact
      301 aatatatttt tatatctatg aataaattat tgaaaaataa aaaaggggag aaggtgatgt
      361 ttttggggaa tgaggcgatc atccgcgggg cgctggaatc cggggtgcaa ttcgtttcca
      421 cctatccggg aacgcccgct tcggagatcg gcaacaattt ttttgccttg caaaaagaat
      481 tgaaagacga tcccaaattt ttgtttgaat tttccaccaa cgaaaagacg gcgatggagg
      541 cggccatcgg cgccaatttt tcggggctca aaaccttggt ggcgatgaaa aatttcggct
      601 tgaatgtcgc cctggaaccg ctcttgccgc tggcctatac cggcacgcgc gcgccgatgg
      661 tgatttttgt ggccgatgac ccggggtgcc acagctcggc gcaaagcgag gaaaacactc
      721 ggccgttttc ggtgttgggg catctgccga tgctggagcc cagcgacgcg caggagtgca
      781 aagatttcac gattgaagcg tttgccattt cggaaaaatt cgggacgccg gtgatcgtgc
      841 gcatcaccac gcgcaccgcg caccagcggg cgctggtggc tttgggcgaa ttggctcccg
      901 accgcaaagt caacctgaaa ggcgaattta tcaaagacaa gcgcaaatat gtgaccatga
      961 tgccgcgcct gcacggaatg aaaaaagaaa tgctggcccg agaagaaaaa ttggcgcagt
     1021 attttgaaaa aagcaaatta aaccgcgttg aaggcgttat taaaggtaaa aatcagccgt
     1081 tgggaataat cacttccggt atcgcttatt tgcacgcgca ggaggcgatg gaagaattgg
     1141 gcgtcggttt gccggtgctg aaattgggga tgtttcatcc tcttccggaa aaaaagatcc
     1201 gtgatttcat caagggattg aaaaaggttt tggtgctgga agaaatggaa ccctatttgg
     1261 aaaagaggat ccagaggctg gccaaggatg ccaattgcaa attggaaatc cgcggggttg
     1321 attggcgccc gcaggtcggc gaaatgaagc cggagatcgc ggccgaaatg attgccaaat
     1381 tcacgggcaa aaaatacact ctgcccaaat tcgcgcccaa atttgaactg cccaaccggc
     1441 cgccgcaatt gtgccccgga tgtccctatt ggctggcatt tgccgcggtg aaaaaagccg
     1501 tggatgccaa caaggtggtt tttggcggcg atatcggctg ttatatgatc ggcgctctgc
     1561 cgccgcatga gctttacgat tatatgtttt gcatggggtc gtcaatcggc attgcccacg
     1621 ggattaaaaa agccgccgcg gatcaaaaag tgattgcctt catcggcgat gccacatttt
     1681 tccatagcgg cattgaagga ttgctcaacg cggtttataa cggctccaac cccttgatca
     1741 ttattatgaa caatgacatc accgcgatga ccggccatca gccgcatccg gactccgtcg
     1801 gcgcggttca tccgattccg attgagaata tggtgctggc ctgcggggtc aagccggaaa
     1861 atatgaaaat cattgaccag ggccaatccg aagagttcat cgccgcggtg cgcgaatttg
     1921 ttgacaagcc cgaagtgagc gtgatcatcg cccgccgccc gtgcaagttt gtggaaaaga
     1981 a
//
LOCUS       DAOQWE010000142         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_67125_length_1979_cov_6.937110, whole genome shotgun sequence.
ACCESSION   DAOQWE010000142 DAOQWE010000000
VERSION     DAOQWE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67125_length_1979_cov_6.937110"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..655
                     /locus_tag="PKK37_04115"
     CDS             128..655
                     /locus_tag="PKK37_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HNR81596.1"
                     /translation="MEEYNSDNIFRNGNFFNPTKGNLSFDDAIGEIFAYMAEEPQREY
                     EIVVGCDSASLEQPHFPATIVVRRMGAGGRFFLKKISYGGKKFYNFKQRILQEVLLSC
                     ELALVLRETLEMRKLILPDNLKWNFSYIHADVGERGATREMVREVTGLIRGNGFEPMI
                     KPFSFAASAIADKYT"
     gene            797..>1979
                     /locus_tag="PKK37_04120"
     CDS             797..>1979
                     /locus_tag="PKK37_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81597.1"
                     /translation="MAKKVIDIFPPPRRAADTIDAEEIKMPLPADLPEAGFPAGSDSV
                     FNATVREDPKPKITFNRVFSAPDRRKDIFTNDDGPGFLKKWGWRIAAAALAVMAGMYL
                     WDANFARAAVKIWPQTESFAREMHVLVDSAIGEIDTVRAAVPGFVISAERTVSADSPA
                     TGTKSAQGKANGSVKIFNDYGNTQTLIKNTRLMAPVEKFQPPLEGAESPWFLIVETVT
                     LEAKSSAVVEVVAAAPGEKYNIEPSTFSIPGLAGTPQYAFITAQSFERFTGGSNEIVP
                     QVTKEDLENAKIAIQDSAKEEIKKELIAKAREQGLEITDENEIKFDIGAPEISAKAGD
                     NAARIGGQAAAKAEAIAYRKADLETLGRKFVSDQIPEGMICEESTFSVQYQSAGIDKE
                     QK"
BASE COUNT          551 a          424 c          563 g          441 t
ORIGIN      
        1 cattttttta tccaaagggc aatcaggtca ttgatatttt ggggcgaggt ggtagaatac
       61 agaggtgtga agcgaaaagg gaaatgttaa atacaagcac aagaagaaag agttaagcgg
      121 aaaaagaatg gaagaatata attccgacaa tattttcagg aacggaaatt ttttcaatcc
      181 cacgaagggg aatttgagct ttgacgatgc catcggcgag atttttgcgt atatggccga
      241 agaaccgcaa cgggaatatg agatcgtggt gggatgcgat tcggcgtcgc tggaacagcc
      301 gcattttccg gccacgattg tggtgcggcg catgggcgcg ggcgggcggt tttttttgaa
      361 aaaaatcagt tatggcggca aaaagtttta taatttcaaa cagagaattc tgcaggaagt
      421 tttgctttcc tgcgagctgg cgctggtgtt gcgcgaaact ttggaaatgc gcaaactgat
      481 tttgcccgac aatttaaaat ggaatttttc ctacattcac gccgatgtgg gcgaacgcgg
      541 cgccacgcgg gagatggtgc gcgaggttac ggggttgatc cgcggcaacg ggtttgaacc
      601 gatgatcaag ccgttttctt ttgccgcgtc ggcgatcgcg gacaaataca cataaaaatt
      661 taaaaatcaa aaatcaaaat tgaacgcaaa atacggatac aaaaattaaa agataagaat
      721 gttgaatttt caaaccgaac ggtttttgtt ataatcaagc tgtcggagcc gatagttcaa
      781 taatttcaat caatttatgg cgaaaaaggt aattgatatt ttcccgccgc cgcggcgtgc
      841 cgcggacaca attgatgccg aagaaataaa aatgcctctg ccggcggatt tgccggaggc
      901 gggttttccc gccggtagcg acagcgtttt taatgctacc gtccgcgaag atccgaagcc
      961 gaaaataacg tttaaccggg tattttcggc gccggaccgc cggaaagata tttttaccaa
     1021 cgacgatggc ccgggatttt tgaaaaaatg gggatggaga attgccgccg ccgcgctcgc
     1081 ggtgatggcg ggaatgtatc tttgggatgc caattttgcc cgcgcggcag tgaaaatatg
     1141 gccgcaaacc gaaagcttcg cgcgggaaat gcacgttttg gtggattcgg ccatcggcga
     1201 gattgatacc gtgcgggcgg cggtgccggg atttgtcatt tcggcggaaa ggaccgtttc
     1261 ggcggattct ccggccaccg gcacgaaaag cgcccagggg aaggctaatg gttcggtcaa
     1321 gattttcaac gattacggca atacccagac tctcatcaaa aacacccgtc ttatggcgcc
     1381 ggtggaaaaa ttccagccgc cgctggaagg cgcggaatct ccctggtttt taatcgtgga
     1441 aacggtaacg ctggaagcga agtcgtcggc ggttgttgaa gtggtggccg ccgcgccggg
     1501 cgaaaaatac aatattgaac cgtcaacatt ttccattccc ggattggcgg ggacgccgca
     1561 atacgctttc atcaccgcac aatcgtttga gagattcacc ggcggctcca atgaaatcgt
     1621 tcctcaggtg accaaggaag acttggaaaa tgccaaaatc gcgatccagg attcggccaa
     1681 ggaggaaatc aagaaagagt taattgccaa agcgcgggaa cagggattgg aaatcaccga
     1741 tgaaaatgaa attaaatttg atattggcgc gccggagatt tcggctaagg ccggcgataa
     1801 cgcggcgcga atcggcgggc aggcggcggc aaaagcggaa gcgatcgcct accggaaggc
     1861 cgatcttgag acattgggca ggaaattcgt ttccgaccag attcccgaag ggatgatttg
     1921 cgaggaaagc actttcagtg tccaatatca aagcgccggc attgacaaag aacaaaaac
//
LOCUS       DAOQWE010000143         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_68531_length_1955_cov_3.188421, whole genome shotgun sequence.
ACCESSION   DAOQWE010000143 DAOQWE010000000
VERSION     DAOQWE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68531_length_1955_cov_3.188421"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKK37_04125"
     CDS             complement(<1..478)
                     /locus_tag="PKK37_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81598.1"
                     /translation="MNKMKKRYLLILTVAIAALIFAGIWESRRFSKLPPAALENENPT
                     ATTTAENGQNATSTIATFEECVSAGKEVAGEKPNRRCIVSNDLVFVEIETCAAPNGKT
                     MNIYEARHLFGAGKCAWEGSAKDEHSCNETGGAWEIGILAYRKNCAAVCVIDIATKE"
     gene            complement(475..1569)
                     /locus_tag="PKK37_04130"
     CDS             complement(475..1569)
                     /locus_tag="PKK37_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNR81599.1"
                     /translation="MASLERIKIIQKAIKNEELDAFIVNGNANNYYLTGVVDNLIKNT
                     FYLVILKNNFYFIGTNFHLKQIRKKIPQRNIRLADKSAGFGKIIVDILKNLEKIGFES
                     HQFSYAKYENFKKLLAGKKFMPFADIVEQTRSIKSAGEIALIKKAAVITDKTFAELLK
                     LIKPGVAEICLKQKTIEIMNGFGATGIAFDPIVASGKNSADPHYEGSNKKIKAGEMVV
                     IDIGARYKNYDADMTRMVFVGKAPAKYKSLYSLVLQTQQKAIAECKAGALPVQIFENA
                     VNNFAAAGEDEFFTHGLGHGVGIDIHELPNLSPKGQGMLENNMVFTVEPGLYHPGWGG
                     IRIEDLCRMKNGKAEILSRTPKNLIEIQVK"
     gene            1634..>1955
                     /locus_tag="PKK37_04135"
     CDS             1634..>1955
                     /locus_tag="PKK37_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the removal of amino acids from the N
                     termini of peptides; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNR81600.1"
                     /translation="MDKNEQNEPNLMFEKKFAFDIWDDATTKKCFDFCEGYKEFLSAC
                     KTERQTVEAVKNIAAAAGFASLEHIDSGSNDTIAAHKVYSINRGKSIVLARRGKKPIK
                     NGARI"
BASE COUNT          494 a          439 c          415 g          607 t
ORIGIN      
        1 attctttggt ggcgatgtca atcacgcaaa cggcggcgca atttttgcgg taggcaagga
       61 tgccgatttc ccacgcgccg ccggtttcgt tgcaggaatg ttcgtctttg gcgctgcctt
      121 cccaggcgca tttgccggcg ccaaaaagat ggcgggcttc atagatattc attgttttgc
      181 cgttcggcgc ggcgcaggtt tcaatttcaa caaacaccaa atcattactc acgatacagc
      241 ggcggttggg tttttcgccg gccacttctt tgccggcaga aacgcattct tcaaaagtgg
      301 caatggtgga agtggcgttt tggccgtttt cggcggtggt tgtcgccgtt ggattttcgt
      361 tttccaacgc ggcgggcggc aattttgaaa aacggcgcga ttcccaaatc ccggcaaaaa
      421 tcagcgcggc aatcgcaacg gttaaaatca acaaatatct ttttttcatt ttattcattt
      481 tacttgaatt tcaattaaat tcttcggcgt tcggctcaaa atttccgcct tgccgttttt
      541 catccggcac aaatcttcaa tgcggattcc gccccagccg gggtgatata atccgggttc
      601 aacggtgaaa accatattgt tctccagcat cccttggcct tttggggaaa gattgggcag
      661 ttcgtggatg tcaatgccca caccgtgccc caagccatgg gtgaaaaatt catcttctcc
      721 ggcggcggca aaattattga cggcgttttc aaaaatctgc acgggtaatg cgcctgcctt
      781 gcattcggca atcgcttttt gctgggtttg taaaacgagg ctgtaaagac tcttgtattt
      841 tgccggtgcc ttgcccacaa aaaccatccg ggtcatatcc gcgtcataat ttttatatcg
      901 cgccccgata tcaattacca ccatctcccc cgcctttatc ttcttgtttg acccttcgta
      961 gtgcggatcc gccgagttct tcccgctggc cacgatcgga tcaaacgcga ttcccgtggc
     1021 cccaaatccg ttcattattt caattgtttt ttgctttaaa caaatttcgg caactccggg
     1081 tttgatcaat ttcagcaatt cggcaaatgt tttgtcggta atcaccgcgg ctttttttat
     1141 taaagcgatt tccccggcgc tttttatgct acgtgtttgc tctactatat ccgcgaaagg
     1201 cataaatttt tttcccgcga gtaatttttt aaagttttca tatttggcat aactgaattg
     1261 gtggctttca aagccgattt tttctaaatt ttttaaaata tcaacaatta tttttccaaa
     1321 accggcgctt ttatccgcca ggcgaatatt tctttgcggt atttttttcc gaatttgttt
     1381 taaatggaaa tttgttccga taaaatagaa attatttttt aggattacca gataaaaagt
     1441 attttttatt aagttatcaa ccacgccggt caggtaataa ttgttcgcgt taccatttac
     1501 gataaaagca tccaattctt cgtttttgat ggctttttgt attattttta ttctttcgag
     1561 cgatgccata aatttacttt ttgcttaaaa tgagttaatt tcaagaataa cacgattaaa
     1621 tactaccaca actatggata aaaacgaaca aaacgagccg aatttgatgt ttgaaaagaa
     1681 attcgcgttt gatatttggg acgatgccac gaccaaaaaa tgttttgatt tttgcgaggg
     1741 ttacaaagaa tttttaagcg cttgcaaaac cgaaaggcaa acagtggaag ccgtaaaaaa
     1801 cattgccgcc gccgccggat ttgcttcctt ggaacatatt gattccggaa gtaacgatac
     1861 gatcgcggcg cacaaggtat attcaatcaa tcgcggcaag tcaatcgttt tggcaaggcg
     1921 cggcaaaaaa ccgataaaga atggcgcgcg gataa
//
LOCUS       DAOQWE010000144         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_68611_length_1953_cov_3.945205, whole genome shotgun sequence.
ACCESSION   DAOQWE010000144 DAOQWE010000000
VERSION     DAOQWE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68611_length_1953_cov_3.945205"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PKK37_04140"
     CDS             complement(<1..645)
                     /locus_tag="PKK37_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNR81601.1"
                     /translation="MNKNNHMKYAVIGTGFIFRKHLQAINQTGGKIVDVCDITHGRGE
                     AWKNIVKNSEADCTVILAPNYLHYEIAKAANEAGKIVLCEKPLALKSEHIKDLAGREN
                     IFVVQQLRYHPEVKRLEKQIDKNKKYDISIDVSVFRDRHYFDGWKGDFEKSGGPLFTL
                     GVHYFELLLHLFGPALEAKMNKFDGKTGEGIIKGANYDCRWRVSVGARIDESCKR"
     gene            complement(676..>1953)
                     /locus_tag="PKK37_04145"
     CDS             complement(676..>1953)
                     /locus_tag="PKK37_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNR81602.1"
                     /translation="LPHAAEIAGAGMEVIGLDVQEKRVAMLNHGESYISDVSKDALEK
                     IIAAGKFKASADFSLLARADVIIICVPTPLDKYKIPDISYITAAANEVKKYLRRGQLV
                     ILESTTYPGTTEEVLLPLFEETGLKCGKDFYLAFAPERIDPGNKMPLAEIPKVVGGIT
                     PECTNLAATFYSQFLKHIHSVSSAKAAEMTKILENVYRLINISAINELAMLCGRMGLD
                     IWEVIDAAKTKPYGFQAFYPSPRVGGHCIPLDPFYLSWKAKEYNFWPRFIELAGDIND
                     QMPHYMVSFANSVLNRRGKPIKNSKILVFGAAYKKDIGDPREGAVYDVIPNLMRKGAQ
                     VDFYDPFVKELTIGKNNYLINNPAPIKMASIDYAPEILKNYDLTLILTDHSNIDYDQL
                     AQNANLVADTRNAIKSRGHKNVFRLGVPAPKDE"
BASE COUNT          477 a          497 c          431 g          548 t
ORIGIN      
        1 gcgcttacat gattcgtcaa tccttgctcc caccgacacg cgccatcggc aatcataatt
       61 ggctcctttg ataattcctt cgccggtttt tccgtcaaat ttattcattt tcgcctccaa
      121 ggccggaccg aacaaatgca acaataattc aaaatagtga acgcccagag tgaacagcgg
      181 cccgccggat ttttcaaaat cacctttcca accgtcaaaa taatgccggt cgcggaacac
      241 ggaaacatca attgaaatat cgtatttttt atttttatca atttgttttt ccaagcgctt
      301 cacttcggga tggtagcgca gctgctgaac aacgaaaata ttttcccggc cggccagatc
      361 ttttatgtgt tcggatttta acgccagcgg tttttcgcat aaaacgatct tccccgcctc
      421 attggcggcc ttggcgattt cataatgcaa atagttgggc gccaaaatca ccgtacagtc
      481 cgcctcggaa tttttaacga tatttttcca tgcctctccc cgtccgtgcg tgatatcgca
      541 aacatcaaca atcttgccgc cggtttgatt gatcgcctgc aaatgtttgc ggaaaataaa
      601 tccggtgccg ataacggcat atttcatatg attgttttta ttcataattc ataatttata
      661 attcatggtt cccggttatt cgtcttttgg cgcgggaacg cccagccgga aaacattttt
      721 atggccgcgg gatttgatcg cattgcgcgt atcggccacc aaattcgcgt tttgcgccaa
      781 ttggtcataa tcaatgtttg aatgatcggt taaaatcagc gtcaaatcgt aatttttcaa
      841 aatttcggga gcataatcga ttgacgccat ttttatcggc gcgggattgt ttatcaggta
      901 attgttcttg ccgatggtca gttccttgac aaacggatcg taaaaatcaa cctgcgcccc
      961 tttgcgcatc aaattgggaa tgacatcata taccgcccct tcgcgcggat cgccgatatc
     1021 ttttttatag gccgcgccga aaaccaaaat ttttgaattt ttaatcggct tgccgcggcg
     1081 gttcagcacc gaattggcaa acgacaccat atagtgcggc atttgatcgt tgatgtcgcc
     1141 ggctaattca ataaaacgcg gccaaaaatt atattctttg gctttccacg aaagatagaa
     1201 cggatccagc ggaatgcaat ggccgcccac gcgcggcgag ggataaaacg cctgaaagcc
     1261 atagggtttg gttttggccg catcaatcac ctcccaaata tcaagcccca tgcggccgca
     1321 cagcatcgcc agttcgttga tcgccgaaat gttgatcagg cggtaaacat tttccagaat
     1381 cttggtcatt tccgcggctt tggccgatga aaccgaatgg atatgtttta aaaattgtga
     1441 gtaaaacgtt gccgccaaat tggtgcattc cggcgttatc ccgcccacca ctttgggaat
     1501 ttcggctaac ggcattttgt tccccggatc aatccgttcg ggcgcaaacg ccagataaaa
     1561 atcctttccg cattttaacc ccgtttcttc aaacagcggc agtagcactt cttcggtggt
     1621 tcccggatag gtggtgcttt ccaaaatcac cagctggccg cggcgcaaat attttttcac
     1681 ctcgttggcc gcggcggtga tgtaggaaat gtcgggaatc ttgtatttgt ccagcggcgt
     1741 gggcacgcaa ataataatca cgtcggcccg cgccagcaaa gaaaaatcgg ccgacgcttt
     1801 gaattttccg gcggcaatga ttttttccag cgcgtctttt gaaacgtcgc tgatatagga
     1861 ttcgccgtga ttgagcattg ccactctttt ttcttgcaca tccaaaccga ttacttccat
     1921 cccggcgccg gcaatttccg ccgcatgggg cag
//
LOCUS       DAOQWE010000145         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_71232_length_1909_cov_7.723301, whole genome shotgun sequence.
ACCESSION   DAOQWE010000145 DAOQWE010000000
VERSION     DAOQWE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71232_length_1909_cov_7.723301"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..687
                     /locus_tag="PKK37_04150"
     CDS             16..687
                     /locus_tag="PKK37_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014798775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR81603.1"
                     /translation="MLKGVSFSVDDGEFVAIIGPSGSGKSTLMHILGMLDTPTSGDYF
                     LDGKNVAGYSDDDLAEIRKNKIGFVFQSFNLLPRATVLRNVCLPLVYAGVEKTAREER
                     ARAALARAGLGTEHHQHRTNQLSGGQMQRVAIARALVNNPSIILADEPTGNLDTKTGE
                     IVMETFQSLNETQGHTIILITHEPDIAEYADRIIAVRDGMIVDDRKNQNKKIGIPGVA
                     DRKQI"
     gene            708..>1909
                     /locus_tag="PKK37_04155"
     CDS             708..>1909
                     /locus_tag="PKK37_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR81604.1"
                     /translation="MKISDLLEQTFFSLLANKARSGLTVLGIVIGIGSVIAMVSIGQG
                     ASGTITSSIESLGSNLLMVSPGMQRGVGQQVSAGRGGAQTLTLDDAEAIAEQIEGVKT
                     VAPQYSTRLQITAKGTNTNTTVYGVTDTYGEVRNVEIGSGSFITPAHNNAKSKVAVLG
                     PTTAEDLFGEGIDPIGQTVRFSNMDFKVVGVTAAKGGSGMSNADDAIYIPLSTAQQYI
                     SGNNRVGMINIQGESQEILEQMEADITALLMTRHKITDEASIDFQIMNQADIVESASS
                     IAGTLTILLASIAGISLLVGGIGIMNMMLTTVTERTREIGLRKAVGAKRGDIAVQFLA
                     EAVTLTFVGGVVGTGAGGLASWAVEKFGNTATEISLASVLLAFGVSAVIGIVFGYYPA
                     ARAAKMDP"
BASE COUNT          459 a          470 c          566 g          414 t
ORIGIN      
        1 agtgaagccg ccaaagtgtt gaagggcgtg tcgttttctg ttgatgacgg agaatttgtg
       61 gcgataatcg gaccgtcggg atcgggcaaa tccacgctga tgcatatttt gggaatgttg
      121 gacactccga catcgggaga ttattttttg gacgggaaaa atgttgccgg atattccgat
      181 gacgatctgg ctgaaatcag aaaaaacaaa atcggtttcg tgttccaatc attcaatctt
      241 ttgccgcgcg ccacggttct gcgcaatgtt tgtttgccgc tggtctacgc gggcgttgaa
      301 aaaaccgcgc gcgaagagag ggcgcgcgcc gcgctggcgc gcgcggggct gggaacggaa
      361 catcatcagc accgcaccaa ccaactttcc ggcggccaga tgcaaagagt ggcgattgcc
      421 cgcgccttgg tgaataatcc gtcaatcatt ctggccgacg agcccaccgg caatttggac
      481 accaagaccg gcgaaatcgt gatggagacc tttcagtcgt taaacgaaac gcaggggcac
      541 acgatcattt taatcactca cgaaccggac attgccgaat atgccgatcg cattatcgcc
      601 gtccgcgacg gaatgattgt cgacgaccgc aaaaaccaaa acaagaagat cggcattccc
      661 ggcgttgccg accggaaaca aatttaactc taaaaacgca tttatttatg aaaatttccg
      721 atttattgga acaaactttt ttttcccttc ttgccaataa agcccgttcg ggattgacgg
      781 ttttgggcat tgtcatcggc atcggttcgg tgatcgcgat ggtttccatc gggcagggtg
      841 catcggggac catcacttcc agcattgaat cgctgggatc aaacttgctg atggtgtcgc
      901 ccggaatgca gcgcggcgtg ggccagcagg tgagcgccgg cagaggcggc gcgcaaacac
      961 tgacgctgga cgacgcggag gcgattgccg aacagattga gggagtgaag acggtggcgc
     1021 cgcaatattc cacgcgattg cagatcaccg ccaaggggac caataccaac acgacggtct
     1081 atggagttac cgacacttac ggagaggtgc gcaatgttga aattggaagc ggttcgttca
     1141 tcacgccggc gcacaataac gccaaatcaa aagtggcggt gctggggccg acgaccgcgg
     1201 aagatttatt cggcgagggg attgatccga tcggccagac ggttcgtttc agcaatatgg
     1261 attttaaagt tgtcggagtt accgccgcca agggcgggag cggaatgagt aatgccgacg
     1321 acgcaattta tattccgcta tcaaccgccc aacaatatat ttcgggcaac aaccgggtgg
     1381 gaatgatcaa cattcagggg gaaagccagg aaattttgga gcaaatggag gccgacatca
     1441 cggcgctgtt gatgacgcgg cataaaatca ccgacgaggc cagcattgat ttccaaatta
     1501 tgaatcaagc cgatatcgtg gaaagcgcgt cgtcaattgc cggcacatta acgattttgc
     1561 tggcgtcaat cgccggcatt tcgctgttgg tgggcggcat cggcattatg aatatgatgc
     1621 tcaccaccgt taccgagcgc acgcgcgaga tcggtttgcg caaggcggtg ggcgccaaaa
     1681 gaggcgatat cgcggtgcaa tttcttgccg aagcggtaac gctcactttt gtgggcggcg
     1741 tcgtcggcac gggcgcgggc ggactagcca gttgggcggt ggaaaaattc ggcaataccg
     1801 ccacggagat ttccttggcg tcggtcttgc tggcgttcgg cgtgtcggca gtgattggta
     1861 tcgttttcgg ttattatccc gccgcgcgcg cggccaaaat ggatccgat
//
LOCUS       DAOQWE010000146         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_71798_length_1900_cov_5.101355, whole genome shotgun sequence.
ACCESSION   DAOQWE010000146 DAOQWE010000000
VERSION     DAOQWE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71798_length_1900_cov_5.101355"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="PKK37_04160"
     CDS             <1..835
                     /locus_tag="PKK37_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNR81605.1"
                     /translation="VGWLEYNDDCTMNAEKSCAKILEYRPRFIVIGGSVILFPQPIKE
                     LREACDKVGARIIYDAAHVLGLIAGGMFQDPLREGADIITTSTHKTFPGPQGGMILGN
                     VGEETQKKIQTAVFPQFSSNHHLHRMPALYGALREMQTFGKDYAGQIVKNAQALAREL
                     DSLGFNVLAKERGFTRSHQVLVNCGQPGSGGVAAAALERANIILNKNIIPGDGVDPRN
                     PRGIRIGVQEMTRFGMKEDAMLQIAEFIKRVVLDKENPETVGKEVAAFRAGFQGVKYC
                     F"
     gene            854..1768
                     /locus_tag="PKK37_04165"
     CDS             854..1768
                     /locus_tag="PKK37_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81606.1"
                     /translation="MKKITIAATTLFALSCARTAAAQLYTCTGQNDACTLCDFFAMMV
                     KVIGITLKFVVPSAIILAVIAAGVMMMLKQEDPDVWARAKKIMLTAIIGGLVAYGGWL
                     LVSVSLAGMNSFNKDTWWKFELVCTHCGDGVLQAGEECEPGISEVPCDEFCKIIVEQK
                     PVVQDRCGTADGGKSLTEPGVSFDIVRPPRSPSKDWRAWGYGDTYTCDEVCAARGQKC
                     IGVGLTNVAVNACVSVVHHIGVNCELSENLQQGSGCSDTYRVLIDATCLEYSNPCKPE
                     WEPCGAAGGDDHEEHPAFFVGETACYCQ"
BASE COUNT          452 a          449 c          540 g          459 t
ORIGIN      
        1 agtgggatgg ttggaatata acgatgattg cacaatgaac gcggaaaaat cctgtgccaa
       61 aattttggaa tataggccgc ggtttatcgt tatcggcgga tcggtgattc tttttcctca
      121 gccgatcaaa gaattgcgcg aggcatgcga caaagtcggc gccagaatca tctatgacgc
      181 ggcgcatgtt ttgggattga tcgccggtgg gatgtttcag gatccgttgc gagagggcgc
      241 cgatattatc accacctcca ctcataaaac ttttcccggt ccgcagggag ggatgatttt
      301 gggaaatgtg ggcgaagaaa cgcaaaagaa aattcaaacg gcggtattcc cgcagttttc
      361 ctccaaccat catttgcacc gtatgcccgc gctttacggg gcgctgcgcg aaatgcagac
      421 attcggcaaa gattatgccg gacagatcgt taaaaacgcg caggcattgg cgcgggaatt
      481 ggacagtttg ggctttaatg ttttggctaa ggagcgcggc tttacccggt cgcatcaggt
      541 gctggtgaat tgcggccagc cgggttcggg gggtgttgcc gccgccgcgc tggagcgcgc
      601 caatataatt ttgaacaaaa acatcattcc cggagacggc gttgatccgc gcaatccgcg
      661 cggcattcgc atcggcgtgc aggaaatgac aagatttgga atgaaagaag acgcaatgtt
      721 gcagattgcc gaatttatca agcgcgtggt gttggacaaa gaaaatcccg aaaccgtcgg
      781 caaagaagtt gccgcattcc gggccggttt tcaaggagta aaatattgtt tttagcgttt
      841 ttgataccga tcaatgaaaa aaataaccat cgccgccaca acgctctttg ctttgagttg
      901 cgcccggacc gccgcggccc aactctatac ctgcaccggc cagaatgatg cctgcactct
      961 gtgcgatttt ttcgcgatga tggtgaaggt tatcggtatc acgctcaaat tcgtcgtgcc
     1021 gtcggcgata attctggcgg tcatcgccgc gggcgtgatg atgatgctca aacaggagga
     1081 tcccgacgtc tgggcccggg ccaaaaaaat aatgcttacc gccatcatcg gcggtttggt
     1141 tgcctacggc ggctggcttt tggtgagcgt gtctctggcg gggatgaatt cttttaacaa
     1201 agacacttgg tggaaatttg aactggtctg cactcattgc ggcgacggcg ttctgcaagc
     1261 gggggaggaa tgcgaaccgg gaatttcgga agttccctgc gacgaattct gcaaaatcat
     1321 cgttgaacaa aagccggttg tccaagatcg ttgcggcacc gccgatggcg gcaagagtct
     1381 caccgagcca ggggtgagtt ttgacattgt gcgaccgcca cgttccccat ctaaagattg
     1441 gagggcatgg ggttatggtg acacttatac ttgcgatgaa gtctgcgccg cgcgcggcca
     1501 gaagtgcatt ggcgtgggct tgaccaatgt cgccgttaat gcttgcgttt cggtggtgca
     1561 tcatattggc gttaattgcg agttgtcgga aaatttacag caaggtagcg gttgttccga
     1621 tacctatagg gtgttaatcg acgcgacatg tcttgaatat tccaacccct gcaaaccgga
     1681 atgggaaccg tgtggtgccg ctggtggtga tgaccacgaa gaacatccgg catttttcgt
     1741 gggtgaaacc gcgtgctatt gccagtgaaa tctaaaattg acatcatatt ttcaaccaat
     1801 gagaaaaata gttatcatta ctacaacctt tcttgtgttg agctacgccc ggaccgccgc
     1861 ggcccaactc tatacctgca ccggccagaa tgatgcctgc
//
LOCUS       DAOQWE010000147         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_72799_length_1884_cov_9.529798, whole genome shotgun sequence.
ACCESSION   DAOQWE010000147 DAOQWE010000000
VERSION     DAOQWE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72799_length_1884_cov_9.529798"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..111
                     /locus_tag="PKK37_04170"
     tRNA            41..111
                     /locus_tag="PKK37_04170"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:73..75,aa:Ala,seq:cgc)
     gene            314..955
                     /locus_tag="PKK37_04175"
     CDS             314..955
                     /locus_tag="PKK37_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNR81607.1"
                     /translation="MISLFPKDGAFYQLFEKQAEKIGEAARLLSVTVKDPQKIKEVAA
                     KLKVLEQSADEIGHELIENLRRNFITPLDGEDIDLLRQNLDDIIDFIERAVNRISIYH
                     IVPPFPEEMENYVRIIIAAGREIKDGVREIKNVKKFRESLHHRCRRLNDLENQGDEIN
                     RRALGGLMAARDVSCGKLIEIIKLKEIYETLENAIDCCENVGNMFESILIKNQ"
     gene            980..>1884
                     /locus_tag="PKK37_04180"
     CDS             980..>1884
                     /locus_tag="PKK37_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNR81608.1"
                     /translation="MTPDLIFIIFIIFTALVFDFTNGMHDAANSISTVVSTRVLAPKY
                     AVLWAAFFNFIAFMIFGTAVAKTIGAGMVDIKTITPVVIFCGLLGAIGWNFFTWCKGL
                     PTSSSHALIGGYAGAAVANAGSGVILWAGWTKTLLFIVVAPTIGLGLGYLFKVASAWV
                     TYKRSPAVVNRWSRILQLFSAAFYSLGHGGNDAQKTMGIITGLLFAGGLIGAFEVPLW
                     VVLSAHTAIALGTLSGGWRIVRTMGQKITKLKPIDGFCAETASAISIFWATHLGVPVS
                     TTHVITGAISGVGAARRLSAVRWNMT"
BASE COUNT          425 a          427 c          544 g          488 t
ORIGIN      
        1 ttgaaaattg attattgaaa attgcgcgtt atcgcgcctt gggcctgtag tcaagtggta
       61 agacgccgca ttcgcattgc ggaagcgcga gtccgattct cgccaggtcc accggcaaat
      121 cttgttgaat gttttgccaa aggggttgcg ttcgcaagtg cgttgttaga tggtattatg
      181 actgaaattt tgaaaaatcg tcgcgactcc ggcaaatcgt ttaccgataa acgcattgcc
      241 ggtttggctt ttggttttta aatttgtttg aaagtcagcc cgggcggctg attttttggc
      301 atttgaacac aatatgatca gtttatttcc caaagatggt gctttttatc agctttttga
      361 aaaacaagcc gagaaaatcg gcgaagccgc gcggcttttg tcggtgaccg tcaaagatcc
      421 gcaaaaaatc aaagaggtcg cggcaaaact gaaagtgctg gaacagagcg ccgatgaaat
      481 cggccatgaa ttgattgaaa atctgcgccg caatttcatc acgccgctgg acggggaaga
      541 tattgattta ctgcgccaga atcttgatga tatcattgat ttcattgaaa gagcggtcaa
      601 tcggatttca atatatcaca ttgtgccgcc gtttcccgaa gagatggaga attatgtccg
      661 gataattatt gccgccggcc gcgagatcaa ggacggcgtg cgggagataa aaaacgtgaa
      721 aaaattccgg gaaagcctgc accatcgttg ccgccggctg aatgatctgg aaaatcaggg
      781 ggatgaaatc aaccgccggg cgctgggcgg attgatggcg gcgcgggacg tgtcctgcgg
      841 caaactgatt gagatcatca agctcaagga aatttacgaa accttggaaa atgcgattga
      901 ctgctgtgaa aacgtgggca atatgtttga gtcaatattg atcaagaacc aataaccgcc
      961 gccggccggc aaccaagcaa tgactccgga tttgattttt atcattttta tcattttcac
     1021 ggcattggtg tttgatttca ccaacgggat gcatgatgcg gccaattcaa tctccactgt
     1081 ggtttccaca agagtgctgg cgccgaaata cgcagttttg tgggcggcgt ttttcaactt
     1141 tatcgcgttt atgatttttg gcaccgcggt ggccaagacc atcggcgcgg gaatggttga
     1201 tatcaagacg attaccccgg tcgtgatctt ttgcggcctg ctgggcgcca tcggctggaa
     1261 ttttttcacc tggtgcaagg gattgccgac cagttcgtct catgcgctga tcggcggtta
     1321 tgccggcgcc gcggtggcca atgccggcag tggcgtgatt ttgtgggccg gctggacgaa
     1381 aacccttctt ttcatcgttg tcgcgccaac gatcggtttg ggtctggggt atttgtttaa
     1441 agtcgcctcc gcgtgggtta cctataagcg ttcgccggcg gtcgtcaacc ggtggtcccg
     1501 gattttgcaa ttgttttcgg cggcattcta cagcttgggt cacgggggca atgacgcgca
     1561 aaagaccatg ggaatcatca ccggcttgct ttttgccggc ggcttgatcg gtgcgtttga
     1621 ggtgccgttg tgggtggtgt tgagcgcgca taccgccatc gctctgggaa cgttgagcgg
     1681 cggttggcgg attgtccgca ccatgggcca aaaaatcacg aagttgaaac ccattgatgg
     1741 attttgcgcc gaaaccgcca gcgcgatcag tattttttgg gcgacgcatt tgggcgtgcc
     1801 ggtgagcacg acacacgtaa tcaccggggc gatttccggc gtgggcgcgg cgcgccggct
     1861 ttcggcggta cgctggaata tgac
//
LOCUS       DAOQWE010000148         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_74269_length_1862_cov_6.441616, whole genome shotgun sequence.
ACCESSION   DAOQWE010000148 DAOQWE010000000
VERSION     DAOQWE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74269_length_1862_cov_6.441616"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKK37_04185"
     CDS             <1..184
                     /locus_tag="PKK37_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81609.1"
                     /translation="YYQQNCLLSLAASLARDFFLIATAQTPLYSLPLNMKIGQKASAR
                     VLTQTAVDIQCGYNVD"
     gene            205..498
                     /locus_tag="PKK37_04190"
     CDS             205..498
                     /locus_tag="PKK37_04190"
                     /inference="COORDINATES: protein motif:HMM:NF016136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNR81610.1"
                     /translation="MSEVIHFRTEKKAKDEAQAIAKKMGLKLSDVLNALLYAFIRTRE
                     LNIRLEEPSDYFIECLEQAREDLKNGDISPAFDNAEDAIKWLHDKNRKYANQI"
     gene            482..742
                     /locus_tag="PKK37_04195"
     CDS             482..742
                     /locus_tag="PKK37_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HNR81611.1"
                     /translation="MQIKFSKFFLKQFAKAPELIVAAFDQKMQIFVLDKYNPILNNHQ
                     LKGKYEGCRSININGDWRAIFEELGNGDIYFEFIGTHSQLYK"
     gene            756..1538
                     /locus_tag="PKK37_04200"
     CDS             756..1538
                     /locus_tag="PKK37_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HNR81612.1"
                     /translation="MVVIRKVTKYGNINELVDFLALGVKEIFGQNLVGFYLTGSLTYG
                     DFEPGRSDIDLAAVLETPASPEQINKIGKLHLEAEQKFRQWTNRVECSYIPRLMLAGI
                     LPPKEPRPYFGAGIFYPAADYGNEWLINRHFLYQNGVVVVGPDFKTLAAPVETKNLKE
                     ACIRDLRQEWAPKINDENFLANSHYQSYVVLNLCRILYMIVNDEAGSKKVAANWAREK
                     YPQFANLIETAQAWHYGIEMNCLAQTIEFIKFAVTSTENIKD"
     gene            complement(1652..>1862)
                     /locus_tag="PKK37_04205"
     CDS             complement(1652..>1862)
                     /locus_tag="PKK37_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81613.1"
                     /translation="LAELSGVNHLAKIFIFLGDIFNIARKSFHIFIFYYLFNLIPDLY
                     LTLPSSAIIVQYKTIYSLSKSASFI"
BASE COUNT          583 a          329 c          429 g          521 t
ORIGIN      
        1 ttattaccaa caaaattgtc tcctttccct cgccgcttcg ctcgctcggg acttctttct
       61 gatcgcaacc gctcagacac ccctctattc gcttccatta aatatgaaaa tcggccaaaa
      121 agcaagtgcg cgcgtcttga cacaaactgc ggttgatata caatgtggat acaatgtaga
      181 ttaattttaa ataatcataa aattatgagc gaagttattc atttcaggac cgaaaaaaaa
      241 gccaaggacg aagcgcaggc aatcgccaaa aaaatgggtt tgaaattgag tgatgtttta
      301 aacgcgcttt tgtatgcctt tatccgcacc agagaattga atattcggtt ggaagagccg
      361 tcggattatt ttattgagtg cttggaacaa gccagagagg atttaaaaaa tggcgatatt
      421 tctcccgctt ttgataatgc cgaggatgcg attaagtggt tacatgataa aaatcgcaaa
      481 tatgcaaatc aaatttagca aatttttttt gaaacaattc gccaaagcgc ctgaattgat
      541 tgttgcggcg tttgaccaaa aaatgcaaat ttttgtgttg gataaatata atccgattct
      601 taataatcat caattgaaag gtaaatatga aggttgccga agtataaata taaatggaga
      661 ttggcgggcg atttttgaag aattgggaaa cggcgatatt tactttgaat ttatcggcac
      721 tcacagccaa ttgtacaaat aatttctaga attttgtggt ggttatcaga aaagttacaa
      781 aatacggcaa tattaatgaa ctggtagatt tcttggcgtt gggggtgaaa gagatttttg
      841 gccaaaatct ggtcgggttt tatttgaccg ggtcgcttac ctatggcgat tttgaaccgg
      901 ggcgcagtga tattgatctg gctgcggttt tggaaacgcc ggcaagcccg gagcaaatca
      961 ataagatcgg caaattacat ttggaagccg aacaaaaatt caggcaatgg acaaaccggg
     1021 ttgaatgttc ttatatcccg cggttaatgc tggccgggat tttgccgccc aaagagcctc
     1081 ggccttattt tggcgccggg attttttatc cggccgcgga ttatggcaat gaatggctga
     1141 tcaaccggca ttttctttat caaaacgggg tcgtggttgt cggcccggat tttaaaactt
     1201 tggccgcgcc ggtggagacc aaaaatctaa aagaggcgtg tatccgtgat ctgcgccaag
     1261 aatgggcgcc caaaattaac gatgagaatt ttttggccaa cagccattac caatcgtatg
     1321 tggttttgaa tttgtgccgg attctttata tgattgtcaa cgacgaggcg ggctccaaga
     1381 aagttgccgc gaattgggca agggaaaaat atccgcaatt tgcaaactta atcgaaaccg
     1441 cccaagcatg gcattatggc attgaaatga attgtcttgc tcaaacgatt gaattcatta
     1501 aatttgcggt tacatcaacc gaaaacatta aggattagat tataccaggc taatcttgcc
     1561 ccggtcgcga aagagagtgc gtgattgttc ggcttaatcg aggccgattg ctgaaaaatt
     1621 gaaccgattt gtcgtggagg taaagtcaaa actatataaa acttgcggat tttgacaaac
     1681 tatatatagt tttgtattgg acaattatag cggaagaggg tagggttaaa taaaggtcag
     1741 gaattaaatt aaacaaataa taaaaaataa aaatatgaaa ggatttgcgg gcaatattga
     1801 agatatcacc aaggaaaatg aaaattttcg caaggtgatt tacaccggaa agttcagcca
     1861 gc
//
LOCUS       DAOQWE010000149         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_74730_length_1855_cov_6.050556, whole genome shotgun sequence.
ACCESSION   DAOQWE010000149 DAOQWE010000000
VERSION     DAOQWE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74730_length_1855_cov_6.050556"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PKK37_04210"
     CDS             complement(<1..772)
                     /locus_tag="PKK37_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNR81614.1"
                     /translation="MTNPFRPISISLSPNVQSDDVKLAFSLLFQRVRPSENSEGLTLQ
                     TEFAKYLGAKHAYAFNSGRSALMAILAAMELENGSEVLLQAFTCNAAVNPIRWAGLEP
                     VFVDCDQNYNLSAEDLKKKITEKSRVVMVQHTFGLPADMDEIVEICRRNNLILIEDCA
                     HSLGAEYCGRKVGTFGQAAFFSFGRDKMISCVYGGIAVTNDERLGQKIAAYQEKIGYP
                     AKDWTRQQLWHPVLMDKIILPSYSILGKHLLVLFQWFHV"
     gene            complement(778..1749)
                     /gene="rhuM"
                     /locus_tag="PKK37_04215"
     CDS             complement(778..1749)
                     /gene="rhuM"
                     /locus_tag="PKK37_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNR81615.1"
                     /translation="MKKELNREEIAIYKTAKGPQIKANIKKESVWLDAHQIAAVFGID
                     RTVIVKHINNIYLTKELFRQSTCAKIAQVAADGKIRKMNIYNLDMIISVGYRVNSKKA
                     TQFRIWATKTLKDYLVRGYAINEARILQAKEQYFQLRKTIEFLQKKARTKLLAGKENE
                     ILELLGDYAKTLQILEQYDNDAVTLKKGAAGKFSLNYDETIKIIGEIKNGRGNLFGVE
                     YEGKFKGIIGAINQTFGGKELYLSIEEKAAHLLYFTIKDHPFADGNKRIASFLFVYFL
                     DKNDYLLRTNGERKINDNALAALALLIAISDPREKDKMVGIITNLLS"
BASE COUNT          483 a          444 c          353 g          575 t
ORIGIN      
        1 ggacatgaaa ccattgaaaa agaacaagca gatgctttcc caaaattgaa tatgagggca
       61 aaatgatttt atccattaaa acagggtgcc acaattgctg gcgcgtccaa tcctttgccg
      121 ggtagccgat tttctcttga taggcggcga ttttttggcc aagccgctca tcgttggtca
      181 cggctatgcc gccataaacg caggagatca ttttgtcccg gccgaaagaa aaaaacgcgg
      241 cctggccaaa ggtgccaacc ttgcggccgc agtattcggc gcccaaagaa tgagcgcaat
      301 cctctatcaa tatcaaatta ttccggcggc aaatttcaac gatctcatcc atatctgccg
      361 gtaaaccaaa tgtatgctgt accattacca cgcgggattt ttccgtgatt tttttcttca
      421 aatcttctgc cgacaaattg taattttggt cacaatccac aaacaccggt tccaaccccg
      481 cccagcggat gggattgacc gccgcattgc acgtgaatgc ctgcaaaagc acctctgatc
      541 cgttttccag ctccatcgcc gccagtatcg ccattaacgc gcttcgcccg ctgttgaacg
      601 cgtacgcgtg ttttgcgccc aggtatttgg caaactctgt ttggagggtt agaccctctg
      661 aattttcaga gggtctaacc ctctgaaata gcaacgaaaa cgccaatttc acgtcatcgg
      721 actggacatt gggagacaaa gatattgata tgggcctgaa cggatttgtc ataggtttta
      781 agacaataaa ttggtaataa ttccgaccat cttgtctttc tcgcggggat cgctgatggc
      841 aattaataat gccaaagccg ccaacgcgtt atcgttaatc tttctttcac cgtttgtgcg
      901 tagtaaataa tcattcttat ccagaaaata aacgaataag aaggaggcaa tccgcttatt
      961 cccgtcggca aacggatggt ctttaattgt aaaatataat aaatgcgcgg ctttctcctc
     1021 aatggaaagg tatagttctt ttccgccaaa tgtttggttt atcgcgccga taatcccctt
     1081 gaatttgcct tcatactcaa ccccgaaaag attgccccga ccattcttta tctcaccaat
     1141 tatctttatc gtttcgtcgt aatttaacga aaattttcca gccgcgcctt tttttaaggt
     1201 taccgcgtca ttatcgtatt gttccaaaat ctgcaatgtt ttagcatagt cgccaagcag
     1261 ctccaaaatt tcattttctt tgcccgcaag cagttttgtt ctggcttttt tttgcagaaa
     1321 ttcaatggtt ttccgcaatt ggaaatattg ctctttcgcc tgtaaaatcc gggcttcatt
     1381 tatcgcgtac cctcttacca aataatcttt aagtgtcttt gtcgcccata tccggaattg
     1441 cgtcgctttc ttggaattca cccggtaacc cacggaaatt atcatatcca aattgtagat
     1501 attcattttc cgaatctttc catccgcggc aacctgtgca atttttgcac aggttgattg
     1561 ccgaaacaat tcttttgtta gataaatatt atttatatgc ttaacaataa ccgtgcgatc
     1621 aataccaaaa accgccgcaa tttggtgcgc gtcaagccaa acagattctt ttttgatatt
     1681 tgccttgatc tgcggccctt tcgcagtttt atagatcgcg atttcttctc tatttaattc
     1741 ttttttcaag tttttattca ttgggcaacg gtcatatgga tattggccga aacggatttg
     1801 ccataatcgt ttattttagc cgtattttta tcttatctca accattgaca aaacc
//
LOCUS       DAOQWE010000150         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_75099_length_1849_cov_20.118729, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000150 DAOQWE010000000
VERSION     DAOQWE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75099_length_1849_cov_20.118729"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1849)
                     /locus_tag="PKK37_04220"
     CDS             complement(<1..>1849)
                     /locus_tag="PKK37_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81616.1"
                     /translation="KILSRTPVLFVPGLLGTEIKRGDELLWVDPLALTASISDEFLDE
                     IQFNSNLFAVNQELSLAGVIKKKNIPLYWDYDYTQGLMYEFAAQGYDVNEGSENQTFY
                     TFPYDWRYGVSGVYPKPKGTGQMDVTNSVLLGRKIEELAKISPTGKVDVIAHSLGGLI
                     AKKYVLENGNPRIGKLVFLGVPNLGAPLAGRGLIAGTDFGVFGLNPQELKKISQNMPA
                     AYDLLPAKNYFSAKGSWVRILKETDRWGVNETQNLDWTQTRIYLAGAGASNTALTNAA
                     NLHSDEFDADNVYEIMANKSIDSYNIAGCRSATFSTLLDMQNKTGVHQYYDYFEFANG
                     DDTVPFESANRKLAKDENTFYVRDAKHGQMPSAAGIRQKIAGIISQNNISLPNNKIIT
                     RAQLLENERLCRLLGIALRIDSPLAIKVTDQGGNIIEDVAGVGPKNEIPGAMFEINNG
                     KKFVYLPQNENQQYQISLQGEGDGFFTLTARAVEDDLLQEPRVFSLLPVSKNLSGGIE
                     LNGRETIIKIDNDGDGEIDQTISQDETFTINELMADFNRYVAAGLIKNPQRTVILAQL
                     KLLQKEFAALEKLQADNRLPQKAKTAAIAAAGRLINRQIDLLAKEIQLMA"
BASE COUNT          410 a          454 c          462 g          523 t
ORIGIN      
        1 tggccatcag ttggatttct ttggccagca gatctatctg ccggttgatc agcctccccg
       61 ccgcggcaat cgccgcggtt ttggcttttt gcggcaagcg gttgtcggct tgcaattttt
      121 ccaacgcggc aaattctttt tgcaatagtt tcaactgcgc cagaataacc gtgcgttgcg
      181 ggtttttaat cagccccgcg gcaacatagc ggttaaagtc ggccatcaat tcattgatgg
      241 tgaaagtttc atcctgggaa atggtttggt caatttcgcc gtcgccgtcg ttgtcaattt
      301 tgatgattgt ttcccgtccg ttcaattcaa tcccgccgga aagatttttt gacaccggca
      361 agagagagaa aacccgcggc tcttggagaa gatcgtcttc caccgcgcgg gcggtgagag
      421 tgaaaaaacc gtcgccttcg ccttgcaggc tgatttggta ttgctgattt tcattttggg
      481 gcaggtagac gaattttttg ccgttgttga tttcaaacat cgccccgggg atttcatttt
      541 tgggaccgac gccggcaaca tcttcaatga tgttgccgcc ctgatcggtt accttgatcg
      601 ccagcgggct gtcaatgcgg agcgcgatcc ccagaaggcg gcagaggcgt tcattttcca
      661 gcaattgggc gcgggtgatg attttattgt tcggcaaaga aatgttgttt tgtgaaatga
      721 taccggcgat tttttggcgg atgccggcgg cgctgggcat ttgcccgtgc ttggcgtcgc
      781 ggacgtagaa agtgttttcg tcttttgcca gtttgcggtt ggccgattca aacggcacgg
      841 tatcgtcgcc gttggcgaat tcaaaataat cgtagtattg atgaacgccg gttttgttct
      901 gcatatccag cagggtggaa aaggttgccg aacggcaacc ggcgatattg taactgtcaa
      961 tgctcttgtt tgccataatc tcatagacat tgtcggcgtc aaattcgtcg gaatggagat
     1021 tggcggcatt ggtcagggcg gtattgctgg cgcccgcgcc ggcaagatag attcgggttt
     1081 gcgtccagtc aagattttga gtctcgttga ctccccaacg atcggtttct ttgagaattc
     1141 tcacccagct tcctttggcg gaaaaataat tcttggcggg aagcaaatca taggcggccg
     1201 gcatattttg agagattttt tttaattcct gaggattcaa tccgaatacg ccgaaatccg
     1261 tgccggcgat cagccctctt cccgccagcg gcgcgcccaa attcggcacg cccagaaaca
     1321 ccagcttgcc gattcgggga ttgccgtttt ccagaacata ctttttggcg atcaatccgc
     1381 ccaaactgtg ggcgatcacg tccaccttgc ccgtgggaga aatttttgcc agctcttcaa
     1441 tttttcgtcc caacaaaaca ctgttagtga catccatttg gccggttccc ttcggtttgg
     1501 gataaactcc gctgacgcca tagcgccaat cgtaagggaa ggtgtagaaa gtctggttct
     1561 cactcccctc gttgacatca tatccctgcg cagcaaactc atacatcaga ccttgggtat
     1621 agtcatagtc ccaataaagt ggaatatttt tcttttttat tactcccgct aaagatagct
     1681 cttgattgac tgcaaataaa ttactattga attgtatctc gtcaagaaat tcatcactga
     1741 tagacgctgt taaagcaagc ggatctaccc acaataattc atctcccctt ttaatctccg
     1801 ttcccaataa tcccggcaca aaaagcaccg gcgtgcggga taaaatttt
//
LOCUS       DAOQWE010000151         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_75981_length_1836_cov_8.080853, whole genome shotgun sequence.
ACCESSION   DAOQWE010000151 DAOQWE010000000
VERSION     DAOQWE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75981_length_1836_cov_8.080853"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /gene="greA"
                     /locus_tag="PKK37_04225"
     CDS             complement(<1..399)
                     /gene="greA"
                     /locus_tag="PKK37_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNR81617.1"
                     /translation="MENYLTPEGRKKLKAELDTLENVTRRELAEKLNYAISFGDLREN
                     AAYHQAKEQQAFVEGRILELKSIIANAKIIEKSGNDRVRLGSSVILSCGGAKEKYVIV
                     GPDEADIFAGKVSYKSPLGEAVLGKKPGDKV"
     gene            complement(512..1777)
                     /locus_tag="PKK37_04230"
     CDS             complement(512..1777)
                     /locus_tag="PKK37_04230"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNR81618.1"
                     /translation="MKNDKFNCEVAVIGGGPAGMMAAIAAAQRGAKTILLEKNAALGK
                     KLLLTGGGRGNFCNAESDLRKLAANYGQNGPFLFHSFSQFGPKEAIEFFNGLGVKTKI
                     ENNNRVFPKSDRAADILNALIAGLNGLNVEIVCGARITGIEKENNSINKIVLDNRDDI
                     VAKNYIIATGGKSYPQTGSAGDGYLWAQALGHKIDPLSPALVPVGIKEEWIKDLAGVA
                     LENPKISAFQNGKKLFSARGEMLFAHFGVSGPAILNIGGAIAGLAGNGAVFIHLDFLP
                     EIINGELQKKILENFQKNPNKTVKNCLADFVPAALAPAICRVAAVDAEKTANNVEKKE
                     RRRLVETMKNFKLEAGKTFPVESGMITGGGVNVAEIDHKTMRSKIIRNLFFAGEIINV
                     HGPTGGFNLLQCWSTGRLAGREASLAHKK"
BASE COUNT          440 a          517 c          385 g          494 t
ORIGIN      
        1 aactttgtcg ccgggctttt tgccaagaac cgcttcccca agcggcgatt tgtaggaaac
       61 ctttcccgcg aagatgtcgg cttcatccgg gccgacaatg acatattttt cttttgcgcc
      121 tccgcaagac aagatgacgg acgaaccgag gcgcactctg tcgttgccgc tcttttcaat
      181 aattttcgca ttggcgataa ttgatttcaa ttccaaaatc cgtccttcca caaacgcctg
      241 ctgttctttg gcttgatggt aggccgcatt ttcgcgcaaa tcgccaaagg agatggcata
      301 attcaacttc tccgccaact cccgccgcgt cacattttcc aaagtatcca gttccgcctt
      361 caattttttc cgcccctcgg gcgtcaaata attctccata tattaaaatt ttcagtttac
      421 aattttcaat ttacaataaa ttttcaatta atcaatgatt gaaatttgca tgattgaaat
      481 ttgcggcgga agcgccggcc aaatggcccg ctcatttttt atgagcgagg ctcgcctcgc
      541 ggcccgcgag gcggccggtg gaccagcact gcaaaagatt gaagccgccg gtcggcccgt
      601 ggacattgat aatctccccg gcaaaaaaca aattgcggat aattttggaa cgcatggttt
      661 tgtggtcaat ttcggcaaca ttcacgccgc cgccggtaat catccccgat tcaaccggaa
      721 aagttttacc ggcttccaac ttgaaatttt tcatcgtctc taccagccgc cggcgctctt
      781 tcttctcaac attattggcg gttttttcgg catcaaccgc ggcgacacgg caaatcgccg
      841 gcgccaatgc cgcgggaaca aaatccgcca ggcaattttt gacggttttg ttgggatttt
      901 tttgaaagtt ttccaaaatc tttttttgca attcgccgtt tataatttca ggcaaaaaat
      961 caagatggat gaaaaccgcg ccgttcccgg ccaatccggc aatcgcgccg ccaatgttta
     1021 atatcgccgg accgcttaca ccaaaatgag cgaacagcat ttcgccgcgc gccgaaaaaa
     1081 gttttttgcc gttttgaaac gccgaaattt tgggattctc caaggcaacg cccgccaaat
     1141 cttttatcca ctcctctttt atgcccaccg gcaccagcgc cggcgacaac ggatcaattt
     1201 tgtgccccag cgcttgcgcc cacaaataac cgtccccggc ggatccggtt tgcggatagg
     1261 atttgccgcc agtggcgata atataatttt tggcaacaat atcatctctg ttgtctaaaa
     1321 ctattttatt gatagaatta ttttctttct caatgccggt aatcctggcg ccgcaaacaa
     1381 tctccacgtt caaaccgttc aatccggcaa tcagcgcgtt caaaatatcg gcggcgcgat
     1441 cgcttttcgg aaatacccgg ttattgttct caattttcgt tttcaccccc aaaccattaa
     1501 aaaattctat cgcctccttc ggcccaaatt gagaaaacga atgaaataaa aacggcccgt
     1561 tttggccata attggcggcc agcttccgca agtcggactc ggcattgcaa aaattgcccc
     1621 gcccgccgcc ggtgagcaaa agtttttttc caagcgccgc gtttttttcc agcagtatcg
     1681 ttttcgctcc gcgttgagcc gcggcaatcg ccgccatcat tcccgccggc ccgccgccga
     1741 tcacggccac ctcgcaattg aatttatcat ttttcatatt ttccatattg cgcccatatt
     1801 aacaaaaaac cgccccccgt gaaagggcgg gggcgg
//
LOCUS       DAOQWE010000152         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_78638_length_1798_cov_5.573723, whole genome shotgun sequence.
ACCESSION   DAOQWE010000152 DAOQWE010000000
VERSION     DAOQWE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78638_length_1798_cov_5.573723"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKK37_04235"
     CDS             <1..461
                     /locus_tag="PKK37_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR81619.1"
                     /translation="PDVIMIGEMRDAETISTALTAAETGHLVFATLHTNSAPQSIHRI
                     VDSFPAEQQSQVRAQLSASLLGIVSQRLILSNKGKMIPACEVLFNNFAIGNLIRENKI
                     HEIPLVIDTSFKDGMISLNKSLANLVKAGEIDLQKALSYSLNPQELEALI"
     gene            587..1792
                     /locus_tag="PKK37_04240"
     CDS             587..1792
                     /locus_tag="PKK37_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR81620.1"
                     /translation="MKFVYQARTKSGEVKAGSVNAESVSSAAEILRQQGLFITFLKKE
                     DRGVFGSIRFFEGVSGKDVAIFSRQLAVMFAANVSLVESLRTVGGQLSNQSFREKILK
                     IAQIVEAGSPLSDALAKYPDVFSSFFVSMVKAGEVSGNLSEQFNYMADYLEKQYYLAG
                     KVKAAMVYPAVIVSVMVGVLFLLSYFVMPNLTSMLQESETELPAITKAVIATTDFIRG
                     IGGIIVLLCLIAIAVAVFRISRTKAGKRFFDNLSLRVPVFKNLLKMLYVSRFADNLSV
                     LISGGIPITRALEITRDIVGNDLYRDAIRKTAEGVRRGETVSSVLFNYPDLFPSMFSQ
                     MILVGEQTGSMDKSLLVMVNFYEKEADRAIDSMLVLIEPALIIILGLLVGGVVGSVMI
                     PLYNSMNAV"
BASE COUNT          416 a          403 c          475 g          504 t
ORIGIN      
        1 atccggacgt gattatgatc ggggaaatgc gcgatgccga aaccatcagc accgcactca
       61 ccgcggcgga aaccggccat ttggtctttg ccactttgca caccaattcc gcgccgcaga
      121 gcatccaccg catcgttgat tccttccctg ccgaacagca atcgcaggtc cgcgcccaat
      181 tgtccgcgtc tctgctgggc atcgtttccc agcgtttgat tttgtcaaac aaggggaaaa
      241 tgattcctgc ttgcgaggtg ctttttaaca actttgccat tggcaattta atccgcgaga
      301 acaaaattca cgaaattccc ttggtgattg acacttcttt caaggatgga atgatttctt
      361 tgaacaaatc actggccaat ttggtgaagg ccggcgagat tgatctgcaa aaagcgcttt
      421 cctattcgtt gaatccgcag gagttggaag cccttatcta aaaaattcaa aatgtaaaaa
      481 tcaaaaatca aaattaagga atttttatat ataacgttga aatttttttg aaaaaaaatt
      541 tcaactccaa aatttttcat tttgattttt taatttttaa tttgttatga agtttgttta
      601 tcaggccaga accaaaagcg gcgaagttaa ggccggcagt gttaacgccg agtcggtgtc
      661 gtcggcggcg gaaattttgc gccagcaggg gcttttcatc acttttttaa aaaaagaaga
      721 ccggggcgtt tttggcagta tccgcttttt tgaaggagtc tcgggcaagg atgtggcgat
      781 attttcgcgc cagctggcgg tgatgtttgc cgccaacgtg tcgttggtgg aatccttgcg
      841 caccgttggc ggccagcttt caaatcaaag tttccgcgaa aaaatcctca agattgccca
      901 aattgtggaa gccggatcgc cgctatcgga cgcgctggcg aaatatcccg atgttttttc
      961 gtcatttttc gtttcaatgg tgaaggccgg cgaggtttcg ggaaatcttt ccgagcaatt
     1021 caactatatg gccgattatc tggaaaaaca atattatctt gccggaaaag tgaaggcggc
     1081 catggtctat ccggcggtga tcgtttcggt gatggtgggg gtgctgtttt tgttgagcta
     1141 ttttgtgatg ccgaatttga cttcaatgtt gcaagagtcg gaaacggaat tgccggcgat
     1201 caccaaagcg gtgatcgcca ccaccgattt cattcgcggc atcggcggca ttatcgtgtt
     1261 gttatgtctc atcgcgattg cggtggcggt gtttcggatt tcgcgcacca aagcgggaaa
     1321 gcggtttttt gacaatctgt ccttgcgcgt gccggttttc aaaaatcttt tgaagatgct
     1381 ctatgtgagc cgatttgccg acaatctgtc ggtgttgatt tccggcggca ttcccatcac
     1441 ccgcgcgctg gagatcacgc gcgacatcgt gggcaatgat ctctaccgcg acgcgatccg
     1501 caagaccgcc gaaggcgtgc gccgggggga aacggtatcg tcggtgcttt tcaattatcc
     1561 ggatttgttc ccgtcaatgt tttcgcagat gattctggtg ggcgagcaga ccggctcaat
     1621 ggacaaaagt ttgctggtga tggttaattt ttacgaaaaa gaggctgacc gggcgattga
     1681 ttcaatgctg gtgctgattg aaccggcgct gatcatcatt ttgggcctgc tggtcggcgg
     1741 cgtggtcggg tcggtgatga tcccgcttta caattcaatg aacgcggttt gacggcgg
//
LOCUS       DAOQWE010000153         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_78771_length_1796_cov_6.624928, whole genome shotgun sequence.
ACCESSION   DAOQWE010000153 DAOQWE010000000
VERSION     DAOQWE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78771_length_1796_cov_6.624928"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1626)
                     /locus_tag="PKK37_04245"
     CDS             complement(358..1626)
                     /locus_tag="PKK37_04245"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HNR81621.1"
                     /translation="MTQRELTRYRVIKNLIAKLINGTDAAKQLSLSVRQVKRLKARVI
                     IGGAKGIPHKSRNRESNHRTDPELWQTAKDLIEAKYPDFGPTFAHEKLTEIHGLKIGE
                     AAVRNLMIAEKIWTPEKRKKNKQYRCQRERKSSFGEMIQFDGCYHLWLEDRGKEYCLL
                     SGIDDATGKPVKLEFADSESVLDVFAYWKEYIKQYGKPVAIYLDKYSTYKINHKSAKD
                     NHELLTQFQRACKELDIGLITAHSPQAKGRVERPFFTFQDRLVKDMRLQNISTVEDAN
                     KFLKDFWTEDYGKRFGVAPKSKTDLHRKLSGKELANLDAIFSVQSIRQVKNDFTVQFK
                     NCWYQLEKEQPVCVRRQEKILIEERLDGSLAIRLRGKYLNFKQLPAKPEKIKNAPFAL
                     PAKSKAHAPAANHPWRGKFAKLERCEKVFS"
BASE COUNT          390 a          437 c          393 g          576 t
ORIGIN      
        1 gccgcgccga ttccacatat tccctgatta tcatgtccaa tatcgttttt tgccgttcgc
       61 taatccgcat aaattttcag ttttcatttt tcaacttttg cgccagaggc gcatccgcct
      121 ccggcggaca ttgaattttc aatgtttcaa ttgccaaaca aaattcggca tttccgtttg
      181 aacaattggc aaaatgatca tttattgaaa attgaaaact gtttgtgttc tcattctatc
      241 atttccaaat tatcagtcaa cccccccgag tgataatgtc aaatgataat agaaatgtca
      301 taccccggat ttttttagaa atgtcatact tgaaaaaatt ttagcggctt ttgcttttta
      361 ggaaaagacc ttctcgcacc tctccaactt ggcaaattta ccgcgccagg gatgatttgc
      421 cgccggcgca tgcgctttgc ttttagccgg cagggcaaac ggagcgtttt tgattttttc
      481 cggcttggcg ggcaattgct tgaagttcag atacttgccc cgcaatcgaa tcgccagcga
      541 gccgtcaagc cgctcctcaa tgagtatttt ctcttgccgg cgcacgcata ccggctgttc
      601 tttttccagc tgataccagc agttcttgaa ttggacggta aagtcatttt tgacctgcct
      661 gattgattgg accgagaata tggcatccag attcgccaac tctttgccgc ttaattttcg
      721 gtgcaaatcg gtcttgcttt tgggcgcgac gccaaatctt ttgccgtagt cttcggtcca
      781 aaaatctttc aggaatttgt tagcgtcttc aacggtggaa atattctgca ggcgcatatc
      841 ctttaccagg cggtcttgga aggtaaaaaa cgggcgctcc accctgccct tggcttgcgg
      901 gctgtgggcg gtgattaaac caatatccag ttccttgcag gctctctgaa attgggttaa
      961 aagctcatgg ttgtctttgg cgctcttgtg gttgattttg taggtgctgt atttgtccag
     1021 atagatggca accggtttgc cgtattgctt gatatattcc ttccagtagg caaagacatc
     1081 taaaacgctt tcgctgtcgg caaattccag cttgaccggt ttgccggtgg catcgtcaat
     1141 tcccgacagt aaacagtatt cttttccgcg atcttccagc cagagatgat agcaaccgtc
     1201 aaattggatc atttcgccga agcttgattt tctttccctc tggcaacggt attgcttgtt
     1261 ctttttccgt ttctcgggcg tccagatttt ttcggcaatc attaaattcc tgaccgccgc
     1321 ttcgccgatt ttcaatccat ggatctccgt gagcttttca tgggcgaatg tcggaccgaa
     1381 atccgggtat ttggcttcta tcaaatcctt ggcggtttgc cataattccg gatcggtgcg
     1441 atgattgctt tccctgtttc gcgatttgtg cgggatgcct ttggcgccgc cgataatcac
     1501 tctggccttc aaacgcttta cctgtctcac cgacaaggat aattgcttgg ccgcgtccgt
     1561 gccattgatt agtttggcaa tcaggttttt gatcacccga tagcgggtta attctctttg
     1621 tgtcataccg ataatttctt tttccatggt tgatgtgatt agtgttttgc caatcacatc
     1681 ataccgcggg gtatgacatt tctattatca ccgcactatg acattactat tattaaatga
     1741 cacccccccg agtgataacg cgctttgaag cgaccgccca gcgagatgtg gcttta
//
LOCUS       DAOQWE010000154         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_79731_length_1783_cov_7.234375, whole genome shotgun sequence.
ACCESSION   DAOQWE010000154 DAOQWE010000000
VERSION     DAOQWE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79731_length_1783_cov_7.234375"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..437
                     /locus_tag="PKK37_04250"
     CDS             78..437
                     /locus_tag="PKK37_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81622.1"
                     /translation="MSKRQKVWLGIFGAMFLVPEILWGGIRWVVFNIFLTSDFFYNWE
                     IGIISGFKNNILGVIMALVQFTGLIALIYFLMRLKLKINLAIKIIIILVLSFLILSTG
                     ILAWMTLYYLINTPQIG"
     gene            442..756
                     /locus_tag="PKK37_04255"
     CDS             442..756
                     /locus_tag="PKK37_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81623.1"
                     /translation="MKRYLPMFLLLPVSIFLFAYSWAQELPEIIIEDADATTTIDLAT
                     PPADFFVVNEPPFLLPDEVAINKQIDLLIKEIQIAQRQKKITAAAAQSLIEKLGYIKI
                     QL"
     gene            753..1295
                     /locus_tag="PKK37_04260"
     CDS             753..1295
                     /locus_tag="PKK37_04260"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNR81624.1"
                     /translation="MKNFNIIELGEAGSTNVEAKRLAAGGAAPWTVVWARRQAGGYGK
                     GGVPWASPEGGLYFSVILPPGDIADLQIATILAAFCVADAVKENFAVEPMIKLPNDVY
                     LNGKKLCGILTENAVCGEVKSSVIGIGVNTNVESFGRGMEKIATSVKIETGREVDNRV
                     LLRQIVSQMVNIFKKLTVKI"
     gene            1435..>1783
                     /locus_tag="PKK37_04265"
     CDS             1435..>1783
                     /locus_tag="PKK37_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNR81625.1"
                     /translation="MNFEKEIKELEKKLGGLEDSAAAEKQHQKGKLTARERIAALLDA
                     ETFTELDSLAETRFDRLGLDKKKVPGDAVIAGFGKIGGRPVCVYSQDFTKMGGSLGEA
                     HGKKIVKVIEMARQ"
BASE COUNT          499 a          310 c          467 g          507 t
ORIGIN      
        1 gcattttgtc aatatccgta gctcactgtt taagtgaggt tttaaaataa aaatagtcaa
       61 tcaaaaaata aaaaataatg agcaaaaggc aaaaagtttg gttggggatt tttggggcga
      121 tgtttctggt cccggagatt ttgtggggag ggataagatg ggttgttttt aatatttttt
      181 taacatcaga tttcttttat aattgggaaa ttggaataat ttctggattt aaaaataata
      241 ttttgggagt tatcatggcg ctagttcaat ttacaggatt aatcgccctt atttattttt
      301 tgatgcggct taaattaaaa ataaatttag cgattaaaat tattattatc ttggtattat
      361 cgttcttaat tttatctacc gggatacttg cgtggatgac attatattat ttaattaata
      421 cccctcaaat tggttaaaca tatgaagcga tatttgccga tgtttttgct tctgccggtc
      481 tcaatatttt tgtttgccta ttcttgggcg caggaattgc ccgagattat cattgaagat
      541 gcggatgcga cgaccacaat tgatctggca actccgcccg cggatttctt tgttgtcaat
      601 gagcctccgt ttcttttacc cgatgaggtg gcgatcaata agcagattga tttactgata
      661 aaagaaatcc aaattgcgca aaggcagaaa aaaatcactg ccgccgccgc gcaatcgttg
      721 attgaaaaat tgggctatat taaaattcag ttatgaaaaa tttcaatatc attgagttgg
      781 gggaggcggg atccacgaat gttgaggcga aaaggttggc ggccgggggc gcggcgccgt
      841 ggacggttgt ttgggcgcgg cggcaagcgg gtggctacgg gaaggggggc gtgccgtggg
      901 cgtctccgga aggcgggctt tatttttcag tgattttgcc gccgggcgat atcgcggatt
      961 tgcaaattgc caccatttta gcggcgtttt gcgtcgctga tgcagttaaa gagaattttg
     1021 ccgttgagcc gatgatcaaa ctgcccaacg atgtttattt aaacggcaaa aagttgtgcg
     1081 gcatattgac ggaaaatgcc gtttgcgggg aggtgaaatc ttcggtaatc ggcatcggcg
     1141 tcaacaccaa tgttgaaagt ttcggccggg ggatggaaaa aatcgccaca tcggttaaaa
     1201 ttgaaacagg ccgggaagtt gacaatcgcg tccttttgcg ccaaattgta agccagatgg
     1261 tgaatatctt caaaaaatta accgttaaga tttaaaattg aaaaatcaaa aatcaaaatg
     1321 ataatgtaaa atgaaaaatt gggtaatttt aaatttttaa tctgtcattt tggtttttcc
     1381 cgccagaggc gggtgcgcct tgggcgcgga tgtttgcatt tttaatttac tgctatgaat
     1441 tttgaaaaag aaatcaagga attggagaaa aaattgggag gtttggaaga ttcggccgcg
     1501 gcggaaaaac agcaccagaa aggcaagctc accgcccgcg agcggattgc cgcgcttttg
     1561 gacgcggaaa ctttcacgga gcttgattcg ctggcggaaa cccgttttga ccggctgggg
     1621 ctggacaaga aaaaagttcc cggcgacgcg gtgatcgcgg gattcggcaa gatcggcggc
     1681 cggccggttt gcgtttacag ccaggatttc accaagatgg gcggatcact gggagaggcg
     1741 cacggcaaaa aaattgtcaa agtaattgaa atggcgcgcc aga
//
LOCUS       DAOQWE010000155         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_80133_length_1778_cov_3.293093, whole genome shotgun sequence.
ACCESSION   DAOQWE010000155 DAOQWE010000000
VERSION     DAOQWE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80133_length_1778_cov_3.293093"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..585)
                     /gene="cas2"
                     /locus_tag="PKK37_04270"
     CDS             complement(307..585)
                     /gene="cas2"
                     /locus_tag="PKK37_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNR81626.1"
                     /translation="MFIVSYDFEDTRRRTKFSTFLKKYGRRLQYSVFELKNSQRVLEN
                     IQKEVDMKYKPHFTKNDSIVIISVCEACKAKIKRYGYSANEESQVVFF"
     gene            complement(587..1537)
                     /gene="cas1"
                     /locus_tag="PKK37_04275"
     CDS             complement(587..1537)
                     /gene="cas1"
                     /locus_tag="PKK37_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated endonuclease Cas1"
                     /protein_id="HNR81627.1"
                     /translation="MLSLPDFKEKQILFVSSKSAEIEKVRIVNDNICLSKNGKIENRI
                     SCHKIFALFVVGDCSLTSVLVRKCADYGVSIFLLKDNFLNYARILSQAEGNYLLREKQ
                     YLLDEVQELAGAKILVANKISNQIHLLEKIDRKEVAQCLEEIKNNAVKAQNAKDLLGF
                     EGAAGKFFFQEYFKDFDWKRRLPQAKTDIANYLMDIGYTMMFNFTEAMLGIYGFDSYK
                     GIYHRLFFQRKSLACDLMEPFRCVIDKQIAKSYNLGQIKTNDFKMRKGGLSLGFEEQK
                     KYVGIFADAIMDRKEDIFCYVRDYYYWMMNGSNFPEFKLK"
     gene            complement(1542..>1778)
                     /gene="cas4"
                     /locus_tag="PKK37_04280"
     CDS             complement(1542..>1778)
                     /gene="cas4"
                     /locus_tag="PKK37_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas4"
                     /protein_id="HNR81628.1"
                     /translation="RYQLYAQMFCLREMGYVVKSLAIHSLSDNKRYKISLPDGAETLE
                     FEKLIEDMQNYKAEPPLHKNPIKCAQCVYKPLCG"
BASE COUNT          542 a          400 c          287 g          549 t
ORIGIN      
        1 gccattgccg ccccagtcca atatctacaa aagtagaaat tggatatgtc tattagagtt
       61 gaaaaaggtc ttgaaatttt aaaatctaca aaagtagaaa ttggatatgt ctattagaga
      121 gctggattag ctttaatcgg cttaaaagat gcgattaaca gataaatttt gattaacgac
      181 ttaaagccga ttaaagcaac aaaatccata aagaccgagg ttttataacc tcaaaaagtt
      241 agatttctaa tagatacact gcaatttcta ccaatttttc acttttgttt ttgtatctat
      301 tcaattttag aagaacacga cttggctttc ttcattggcg gaatagccat atctcttgat
      361 cttggctttg cacgcctcgc acacgcttat tataacaatg ctgtcgtttt ttgtaaaatg
      421 cggcttatac ttcatatcaa cctccttttg aatgttttcc aacacccgct ggctgttttt
      481 taactcaaac accgaatatt gcaaacggcg gccgtatttt ttcaaaaatg tggaaaattt
      541 cgtgcggcgg cgggtgtcct caaaatcata ggaaacgata aacattttat ttcaatttaa
      601 actccggaaa atttgatccg ttcatcatcc aataatagta atcgcgaaca taacaaaaaa
      661 tatcctcttt gcgatccata atggcatcgg caaaaatacc gacatatttt ttctgctctt
      721 caaatcccaa acttaatccc cctttgcgca ttttaaaatc attcgttttt atttgtccca
      781 aattataaga tttggcaatt tgcttatcaa tgacacaccg aaacggctcc atcaaatcgc
      841 acgccaaaga ttttctttgg aaaaacaatc tgtggtaaat tcccttataa gagtcaaatc
      901 cataaatacc aagcattgct tcggtgaaat taaacatcat cgtgtagccg atgtccatca
      961 aataattggc aatatcggtt tttgcctgcg gcaatctccg cttccagtca aaatccttaa
     1021 aatactcttg aaagaagaat tttcccgccg caccctcaaa ccccagcaga tctttcgcat
     1081 tttgcgcctt gacggcattg tttttaatct cttcaaggca ctgcgcgact tctttgcgat
     1141 caattttttc cagcaaatgg atttgattgg aaattttatt tgccaccaaa atttttgccc
     1201 ctgccaattc ttgaacctca tccagcaaat attgcttttc cctcaacaaa taatttcctt
     1261 cggcctgcga caaaatccgg gcataattca aaaaattatc tttcaaaagg aatattgaca
     1321 cgccgtaatc cgcgcatttg cgcaccaaaa cgctggtcaa cgaacaatca ccgacaacaa
     1381 acagcgcgaa tatcttgtgg caagaaatcc ggttctcaat ttttccgttt tttgacagac
     1441 agatattgtc gttgacaatt ctcacttttt caatctcggc agacttggaa ctcacaaaca
     1501 atatctgctt ttctttgaag tcgggcaagc tcaacatatt tttaaccgca aagcggctta
     1561 taaacgcact gggcgcattt tatgggattt ttatgcaacg gcggttcggc cttgtaattc
     1621 tgcatatcct caatcaattt ctcaaattcc aaagtttcgg ctccatcagg caaagaaatt
     1681 ttgtagcgct tattgtccga aagcgaatga atcgccagcg acttcacaac ataacccatt
     1741 tcccgcaaac aaaacatttg cgcgtagagc tggtagcg
//
LOCUS       DAOQWE010000156         1764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_81139_length_1764_cov_7.299590, whole genome shotgun sequence.
ACCESSION   DAOQWE010000156 DAOQWE010000000
VERSION     DAOQWE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81139_length_1764_cov_7.299590"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PKK37_04285"
     CDS             complement(<1..274)
                     /locus_tag="PKK37_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR81629.1"
                     /translation="MKTENQNINDRNVVWFKEVSKKDVELVGGKNGSLGEMFSQLGEK
                     GVPIPNGFITTSTAFWRYLEFNGIDKKLGDIFNGLDVNNLKQLKKAS"
     gene            complement(460..714)
                     /locus_tag="PKK37_04290"
     CDS             complement(460..714)
                     /locus_tag="PKK37_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81630.1"
                     /translation="MAEKDMRKMIIIISLLLLIMIIYLFVINFLKLPDDAQKGLWSGL
                     LIMPLIIWKIITADPIIFSISVLFVVLMILMKIYKHKYKK"
     gene            complement(722..1396)
                     /locus_tag="PKK37_04295"
     CDS             complement(722..1396)
                     /locus_tag="PKK37_04295"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR81631.1"
                     /translation="METASGKLLNFGIIPAQTKKAFDFLSSQSWLKDSGWYLAGGTAL
                     AMQAEHRKSYDLDFFSTERKFDTKQVLGKLLGDSGWETNVDEENTIYGVLFGAKVSFI
                     AYPFFVPDAEFIKYGAINVLAARDVAVMKIIAISQRGRKRDFFDLYWCAKNIEPLEDI
                     IKRLKKQYPGVAHDYHHILNSMDYFEDAKDDPEPELNFKANWSEVKNYFQTEIPAITK
                     KIMDLK"
     gene            complement(1369..1650)
                     /locus_tag="PKK37_04300"
     CDS             complement(1369..1650)
                     /locus_tag="PKK37_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81632.1"
                     /translation="MDMVKYKNMKDDANMVKTIKFRQSLFWDVDPKKIHPEKNAQYVV
                     ERILDFGNDLEVKWMWDYYDKALIKKIVDKSRCLRSRTKNAWKLLLENY"
BASE COUNT          518 a          400 c          256 g          590 t
ORIGIN      
        1 tgctggcttt tttgagttgt tttaaattat tgacatccaa gccattgaat atatccccta
       61 attttttgtc aatgccgttg aattccaaat agcgccaaaa cgccgtgctg gtggtaatga
      121 acccgttcgg aatcggtacg cccttctcgc ccagttgcga aaacatctcg cccagcgacc
      181 cgtttttccc gccgaccagc tccacatctt tcttggatac ctccttgaac cacaccacat
      241 tccgatcgtt tatattttga ttttctgttt tcatagtatt gacaatttat tttcaatccg
      301 cgatcatttt tttaatcgcc cgccggttcc aaaaccggat aagtcaattt taccacatca
      361 aaaccccaaa tcaaaatcac ccttataatc aaaacatcct tacatacttg aggatgtaag
      421 gatgttcgca accaatatcg gtaaacatta aaaataattt tactttttgt atttatgctt
      481 gtaaattttc attaaaatca tcaaaaccac aaacaaaact gaaattgaaa aaataatcgg
      541 atccgctgtt attattttcc atattataag cggcataatg agcaatccgc tccataatcc
      601 cttttgcgca tcatcgggta attttagaaa attaataaca aaaaggtaaa taatcattat
      661 cagtaaaagc aagctaataa ttattatcat tttgcgcata tccttttccg ccatataaat
      721 tttattttaa atccattatt tttttcgtga tcgcgggaat ttcggtttga aaatagtttt
      781 tcacttcact ccaattcgcc ttgaaattca attccggttc gggatcatct tttgcgtcct
      841 caaaataatc catactgttt aaaatatgat gataatcatg tgccacaccg ggatattgct
      901 tttttaaccg cttgataata tcttccagcg gctcaatgtt tttcgcgcac caatacaaat
      961 caaaaaaatc acgttttcgg cctctttgag agatcgcgat aattttcatc accgccacat
     1021 cgcgcgcggc caaaacattt atcgcgccgt attttataaa ttcggcgtcc ggaacaaaaa
     1081 agggataggc gataaaacta actttggcgc caaataacac gccataaatc gtattttcct
     1141 catcaacgtt ggtttcccag ccgctgtcgc ccaacaattt tcccaaaact tgttttgtgt
     1201 caaattttcg ttccgtgctg aaaaaatcca aatcatatga tttgcgatgt tcggcttgca
     1261 ttgccagcgc cgtgccgccg gccaaatacc agccgctatc ttttaaccaa gattgggacg
     1321 acaaaaaatc aaacgccttt tttgtttgtg cgggtattat gccgaaattt aataattttc
     1381 cagaagcagt ttccatgcgt tttttgtcct agaccgcaga cagcgtgact tatcaacgat
     1441 cttttttatc agtgctttgt cgtaataatc ccacatccat tttacctcca aatcatttcc
     1501 aaaatccaaa atccgctcaa ccacatattg ggcatttttt tctggatgaa tttttttcgg
     1561 atcaacatcc caaaacaaac tctgccggaa tttgattgtt tttaccatat tcgcatcgtc
     1621 tttcatattc ttatatttta ccatatccat tgctttaatc aaacccaaaa ttcaccaaaa
     1681 aacatcctta catacttgag tatgtaagaa tgtttggtga tatatatatt caactggata
     1741 ttatgtcact ttattatttt tttt
//
LOCUS       DAOQWE010000157         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_81593_length_1758_cov_8.114504, whole genome shotgun sequence.
ACCESSION   DAOQWE010000157 DAOQWE010000000
VERSION     DAOQWE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81593_length_1758_cov_8.114504"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1758
                     /locus_tag="PKK37_04305"
     CDS             <1..>1758
                     /locus_tag="PKK37_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81633.1"
                     /translation="RIKTAAEWQIAQTGYDHGFFKCEKECLDSPKNPQYYDDPANKTR
                     QADAQSINLPVVLAWDDISGWQNLGEFKTEFYENAPAAIDYGPKSYRVEIQYEEYSEK
                     DANLHIDKNKNPQGIQTLANGQKIFYKIIPESGLEGFNSRDDGGACFFQTNTTYKWRV
                     RPCCDAAGTQCKTYRDDEGWWTFSTSPASELLGLTDKDKYTRGATDGIAQDPDWNGPA
                     AMEGVDFCSAKLFWCKAKLADSSTDYHRFDKVYNENQPYAFNYQMRVMSSENTSLGAL
                     IEKIEKIPILGGILGFIKNSGVISGIKSRWDEWKGEWSGAASECNKWMESDLEKAAKS
                     ESAVGEYESEACHYLQRNADGTCAKPETLSFSSYFDKITNDASFMPFFSAAEKTSSDR
                     NLFTGDWDGKLKYSWQIKTCFNTSPNTKDEKFPHCNGNIEEDYGQKWQLIGKPMSSGT
                     VGKPTATAPADNRDWADENNLVGIGGTLNWTAPCGANSFLYDIQDETGKSIFDGDQIC
                     GADYKKDSGRRIGKSQIAITLTEQEPEQDQDPGAVQLKIDTKYKWRVKSCWPSIPVGE
                     VKNICSGQWSDYWSFRTTG"
BASE COUNT          510 a          456 c          448 g          344 t
ORIGIN      
        1 gcgcatcaag accgccgcgg agtggcaaat cgcgcaaacc ggctatgacc acggcttttt
       61 caaatgcgaa aaagaatgcc ttgattcgcc gaaaaatccg caatattacg acgatccggc
      121 caacaaaacc cgacaggccg acgcgcaatc aatcaatctg ccggtggtgc tggcatggga
      181 tgacatctcc ggatggcaaa atttgggcga atttaaaacg gagttttacg agaacgcgcc
      241 ggcggccatt gattacgggc cgaaaagcta ccgggtggaa atacaatatg aagaatattc
      301 cgaaaaagac gcgaacctgc acattgataa aaacaaaaat ccgcaaggca tccaaacgct
      361 ggccaacggc caaaaaattt tttacaagat catccccgaa agcgggcttg aaggattcaa
      421 ttcccgcgac gacggcggcg cgtgtttctt ccagaccaac acaacctaca aatggcgcgt
      481 gcgcccctgt tgcgatgccg cgggaacgca atgcaaaacc taccgcgatg atgaaggctg
      541 gtggacattt tccacgtcgc cggcaagcga gcttttgggt ctcaccgaca aggacaaata
      601 tacgcgcggt gccaccgacg gcatcgccca ggatccggat tggaacggtc cggcggcgat
      661 ggaaggcgtg gatttttgca gtgccaagct tttttggtgc aaggccaaat tggcggattc
      721 ttcaaccgat taccaccgct ttgacaaagt ctacaacgaa aaccaaccct atgccttcaa
      781 ttaccaaatg cgcgtgatgt ccagcgaaaa cacttctctg ggagcgttga ttgaaaaaat
      841 tgagaagatc cccattttgg ggggaattct cggttttata aaaaacagcg gcgttatcag
      901 cggcatcaaa tcgcgctggg acgaatggaa gggcgagtgg agcggcgccg ccagcgagtg
      961 caacaaatgg atggaatccg atttggaaaa agccgcgaaa agcgaatccg ccgtcgggga
     1021 atacgaatcc gaagcttgcc attatttgca aagaaacgcc gacggcactt gcgccaagcc
     1081 ggaaacgctc tctttttcgt cttattttga caaaattacc aacgacgctt cgtttatgcc
     1141 gtttttctcc gccgcggaaa aaaccagcag tgaccgcaat cttttcaccg gcgactggga
     1201 cggtaagtta aagtattcat ggcaaattaa aacctgcttt aacaccagtc cgaataccaa
     1261 agatgaaaaa tttccccact gcaacggcaa cattgaagaa gattacgggc aaaagtggca
     1321 gttaatcggc aaaccgatga gcagtggcac cgtcggcaaa ccaaccgcca ccgcgccggc
     1381 ggacaaccgg gattgggccg acgaaaataa tttggtcggt atcggcggca ctcttaattg
     1441 gaccgccccc tgcggcgcca attccttttt atatgacatc caggacgaaa ccggcaaaag
     1501 tatttttgac ggcgaccaaa tttgcggcgc ggattacaaa aaagattcgg gaaggcggat
     1561 cggcaaatcc cagattgcaa tcaccctcac cgaacaagaa cccgagcaag atcaagatcc
     1621 cggcgccgtg caattgaaaa ttgacaccaa atacaagtgg cgagtgaaat cctgctggcc
     1681 gtccatcccg gtgggagaag tcaaaaacat ttgcagcggc caatggtccg attattggtc
     1741 ttttcgcacc accggaag
//
LOCUS       DAOQWE010000158         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_82067_length_1752_cov_6.718326, whole genome shotgun sequence.
ACCESSION   DAOQWE010000158 DAOQWE010000000
VERSION     DAOQWE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82067_length_1752_cov_6.718326"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..844)
                     /locus_tag="PKK37_04310"
     CDS             complement(119..844)
                     /locus_tag="PKK37_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR81634.1"
                     /translation="MEIISFQNISKRFKLPHEKKNTLKEYLFFVAKSKSYEELWALRQ
                     IDFSVKKGEFISFIGANGSGKSTLLKLISKVYLPTAGKIRVGGTIAPFLELGVGFQYD
                     LSVKDNVYLYGATLGLSRDKINGIFDTIVDFARLGKYIDQKVKNLSTGMEVRLAFSIA
                     AHADADILLVDEVLAVGDQIFQQKCFDLFTRFKKEGKTIIFVSHDLGIVEKFSDKVGW
                     INNGGLEIMDQPEKVIRIYAKAL"
     gene            complement(852..1613)
                     /locus_tag="PKK37_04315"
     CDS             complement(852..1613)
                     /locus_tag="PKK37_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR81635.1"
                     /translation="MNFHQSANLAAELIRTDFKLRYHGSYLGYLWSFLKPLMMFGVLY
                     VVFSKFLRIDYPDYPFHLFLGIILWNFFAEATTGAMSSIIEKGGLIKKIYFPREIIVF
                     SSVAVSFVVLISNLAVFGIFYLFANLAFDLNMLLFLLYLLQLCIVTLGVAFFLSALYP
                     KLRDLFHIWQVLLQVGFWATPITYILQIVPQKYTTLILANPLAGIINNSRLALINHQL
                     PDVGSFLYTLAAGVILLASGYFLFFRRAPFFAEEV"
BASE COUNT          566 a          358 c          409 g          419 t
ORIGIN      
        1 tttcgctttt ccgaccaagc aatatttgaa gtgtagcaat agctacattg aaaatattgc
       61 gaccgagaaa aagcgaaaga tggggcgaag gtacaagcaa gattgagaag ttatttcttt
      121 acaatgcctt agcgtatatc cggattactt tttcgggttg gtccatgatt tcaagaccgc
      181 cgttatttat ccagccgact ttatcggaaa atttttccac gatgccaaga tcgtgactga
      241 caaaaataat cgttttgcct tcctttttaa aacgggtaaa aagatcaaag catttttgct
      301 ggaaaatttg atcccccacc gccaaaactt catcaacaag caggatgtcg gcatcggcat
      361 gggcggcgat ggagaaagca agccggacct ccatccccgt ggacaaattt ttcacttttt
      421 ggtcaatata ttttccaagc cgggcgaaat caacgatcgt gtcaaaaata ccgttaattt
      481 tatcgcggga caggccgaga gtggcgccat aaagatagac gttgtccttg acgcttaaat
      541 catactgaaa accgacgcca agctcaagga agggagcaat ggtgccgcca acccgaattt
      601 tgccggcggt gggcaaataa acttttgaaa tcagcttcag caaagtgctt ttgccgctgc
      661 cgtttgcacc gataaaactt ataaattcgc cttttttaac ggaaaaatca atttggcgga
      721 gcgcccacaa ttcttcgtag cttttgctct tggcaacaaa aaaaagatac tctttgaggg
      781 tgtttttctt ttcatgggga agcttgaatc tctttgaaat gttttgaaag ctgataattt
      841 ccatataata atcagacttc ttcggcaaaa aacggcgccc gccggaaaaa taagaaataa
      901 ccggacgcga gaagaataac tcccgcggcc aaagtgtata aaaaggaacc gacgtcggga
      961 agctggtggt taatcagcgc gagccgggag ttgttgataa tgccggcaag ggggtttgcc
     1021 aagataagcg tggtatattt ctggggaacg atttgcaaaa tataggttat cggggtcgcc
     1081 caaaaaccga cttggagcaa tacctgccaa atatggaaaa gatcgcggag cttgggatag
     1141 agcgcgctca ggaaaaaagc gacgcccagg gttacaatac acagttgcaa caaatataac
     1201 aaaaaaagga gcatattcaa atcaaaggca agattggcga aaagataaaa aataccgaat
     1261 acggcgagat tggatatgag aacaacaaag gaaaccgcca cggaagaaaa aacgatgatt
     1321 tcgcgcggga aatagatttt cttaatcaag ccgccttttt caataattga ggacattgcg
     1381 cccgtggtgg cttcggcaaa aaaattccat aaaataattc ccaaaaacag atggaacggg
     1441 taatcaggat agtcaatgcg caaaaatttg gaaaaaacca cataaagcac gccaaacatc
     1501 atcaggggct tgagaaacga ccacaagtat cccaaataag atccgtggta gcgaagttta
     1561 aaatcggtgc gaatgagttc ggcggcaaga ttggcgcttt ggtgaaaatt catggcaacg
     1621 ggaccgttaa gaaggctaac gtcagtataa catattcggc cgatgtgatc ctcaaatatc
     1681 ttcggtttta tgattggcga tgaaatagta tttggcggct tcaatcaacc ccacgttggc
     1741 aatggccagt cc
//
LOCUS       DAOQWE010000159         1745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_82605_length_1745_cov_3.569822, whole genome shotgun sequence.
ACCESSION   DAOQWE010000159 DAOQWE010000000
VERSION     DAOQWE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82605_length_1745_cov_3.569822"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PKK37_04320"
     CDS             complement(<1..428)
                     /locus_tag="PKK37_04320"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HNR81636.1"
                     /translation="MTNNGAKIKYLLDTNVLIEFSVWKPISLNLNKDFWTGFETALRK
                     NDWILLDVVAGEVKYDNDLIGWINNQKKNGLVEKIKTDDRNRAAEINNSYKMIDEITF
                     KSTVDTYLIAYAEANNLGIFTRENHRKNMAELYKIPDVCD"
     gene            complement(425..1549)
                     /locus_tag="PKK37_04325"
     CDS             complement(425..1549)
                     /locus_tag="PKK37_04325"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNR81637.1"
                     /translation="MERVKFINRENLRIARENVGLDTLSAARKISQSKSDIVFAFESG
                     DLLPTWPQISKLAKIYEVPELVFFSNEKIEKNKVVPDYRIGQSAENDEKIKKLINLVI
                     KRQKWLKRHIGTETKKNNLQGCGKNIENPEQLAGLIKKNLDIDLDEIKKFSGWNSRKK
                     ALDYLMHKAEDRGIFVGKTISYHMISVRDMRGLYISDDCCPFIIINRKDAVSAQIFSF
                     VHELAHLFRKTESVSNSLEFRQIDENESNEEIFCNKTAVELLLPAADFSKVFYDLADI
                     DSVAEIYKVSKLAIFYRLKSLNKIHCGQASDIEKQIKEELKKNLEIKNSRKSKGGSYI
                     NSMRDSNGNLFNNIVASLYYENKIGYAEASRALNFSVEKI"
BASE COUNT          463 a          404 c          278 g          600 t
ORIGIN      
        1 aaatcgcata catcgggaat tttatataat tcagccatat tttttctatg gttttcccgc
       61 gtgaaaatac ccaaattgtt ggcttcggca taagctatta aatatgtgtc aaccgttgat
      121 ttgaatgtaa tctcgtcaat cattttatag ctgttattga tttccgccgc ccgatttcta
      181 tcgtctgttt taattttttc aaccaatcca tttttctttt ggttgttgat ccatccaatc
      241 aaatcattgt catatttcac ttccccggca acgacatcca gaaggatcca atcgtttttt
      301 cgcagtgccg tttcaaagcc agtccaaaaa tctttgttta gattcagtga aattggtttc
      361 caaaccgaaa attcgattaa aacattggta tccagtaaat attttatttt tgcgccattg
      421 tttgtcatat cttttcaacc gaaaaattaa gcgcgcgcga tgcttcggca tagcctatct
      481 tgttttcata ataaagactg gcaacgatat tattgaaaag atttccgttg gaatctctca
      541 tgctgttaat atagcttccg cccttgctct tcctgctgtt ttttatttca agatttttct
      601 tcaattcttc tttgatttgc ttttcaatat cgctggcttg gccgcaatgt attttgttca
      661 agcttttgag ccggtaaaaa atcgccagtt tactgacctt ataaatttca gccacactat
      721 caatatcggc taaatcgtaa aataccttac taaaatcagc ggcaggcaac aacaactcaa
      781 ctgcggtttt attgcaaaat atttcttcat tgctctcgtt ttcatctatt tgccgaaatt
      841 ccaggctgtt tgacacggat tccgtctttc tgaataaatg agcaagctca tggacaaacg
      901 aaaaaatctg cgccgagacc gcgtctttcc tgttaatgat aataaacggg caacaatcat
      961 ccgaaatata gagccccctc atatcccgca cgcttatcat atgataagat attgttttcc
     1021 cgacaaatat acctctgtct tccgccttat gcatgagata atccaacgct ttttttcgcg
     1081 aattccaccc ggaaaatttt tttatttcat caagatcaat atccagattt tttttgatta
     1141 aaccggccaa ttgttcggga ttttcaatat tttttccgca accttgcaaa ttgttttttt
     1201 ttgtttccgt gccaatatgc cgcttgagcc acttttgcct cttgataaca agattgatca
     1261 attttttgat tttttcgtca ttttccgcgg attggccgat acgatagtcg ggaacgactt
     1321 tgttcttttc aattttttca tttgaaaaaa acaccaattc cggcacttcg taaattttgg
     1381 ccagtttgga aatctgcggc caagtgggaa gcaaatcgcc gctttcaaac gcaaaaacaa
     1441 tatcgctttt tgactgcgaa attttgcgcg cggccgacag cgtatccagt ccgacatttt
     1501 cgcgggcgat cctcaaattc tcccgattga tgaatttaac cctttccata attttcaaca
     1561 gccgttaata ttattatttt atcgtcattt ttaaaccacc gcaacctcaa ccaccgcaac
     1621 ctcaaccccg aaatcatcgc ataagacggt ttttatcccc cgccccctca tccgcactcc
     1681 gcggccgggt atcccaaatt attaaaaaat gggctgaatg agcaagtcat ccagctttat
     1741 ttgtc
//
LOCUS       DAOQWE010000160         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_82748_length_1743_cov_7.480450, whole genome shotgun sequence.
ACCESSION   DAOQWE010000160 DAOQWE010000000
VERSION     DAOQWE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82748_length_1743_cov_7.480450"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PKK37_04330"
     CDS             complement(<1..917)
                     /locus_tag="PKK37_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNR81638.1"
                     /translation="MDELAARPDFWGVPEQAAAISEELADLKKNIGELEDLETELDLL
                     EEAGGEEDIDLAAEKIEKKEELAFLSGKYDRQNAILTIHSGAGGQDAQDWGAMLLRMY
                     SRYCQRQGFKAAVIDESFGDGINDGRIGYKEVSLHIRGPRAYGYLKKENGVHRLVRKS
                     PFNAKNLRQTSFALVEVFPEFSKNDLSGIILRPEDIRIDFYRASGPGGQYVNKRESAV
                     RVTHIPTGVSASCQTERLQGQNKDQAMEILKMKLFHLMQESRENELAKIKGDRVSASW
                     GNQIRNYVLDPYRLVKDTRTGVETSQTDKV"
     gene            complement(1192..1264)
                     /locus_tag="PKK37_04335"
     tRNA            complement(1192..1264)
                     /locus_tag="PKK37_04335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1229..1231),aa:Thr,seq:cgt)
     gene            complement(1465..>1743)
                     /locus_tag="PKK37_04340"
     CDS             complement(1465..>1743)
                     /locus_tag="PKK37_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81639.1"
                     /translation="CKDKGTFQAWQNDEEERVTDCAAAGFRDLFLGLEPPTRSVGLSP
                     VSISTNIAPDAAHRLHHFAGLFKSENSSGGLGTVLNFYNSLKDFPNRR"
BASE COUNT          431 a          477 c          399 g          436 t
ORIGIN      
        1 actttgtccg tttgcgaagt ctccactccg gtgcgcgtgt ccttcaccag ccggtaggga
       61 tcaagcacgt aattgcggat ttgatttccc cagctggccg aaacccggtc gccctttatt
      121 ttcgccaatt cgttttcgcg cgattcctgc atcaaatgaa aaagtttcat cttgagaatt
      181 tccatcgcct gatccttgtt ttgcccttgc aagcgttcgg tttggcagga agcggaaaca
      241 ccggtgggaa tgtgcgtgac gcgcaccgcc gattcccgct tgttcacata ctggccccca
      301 gggccggagg cgcggtagaa atcaatcctg atgtcttcgg ggcgcaggat aattccgctc
      361 aaatcgtttt tggaaaattc cggaaacacc tccaccagcg cgaacgaagt ctgccgcaaa
      421 tttttggcgt tgaaaggaga tttgcgcacc aaacgatgaa cgccgttttc ttttttcaaa
      481 taaccgtatg cccgcgggcc gcgaatatgc agggaaactt ccttgtaacc gatccgcccg
      541 tcgttgatgc catcgccgaa agattcgtca atcaccgcgg ccttgaatcc ctgccgctgg
      601 caatagcgcg aatacatccg cagaagcatc gcgccccaat cttgcgcgtc ctgcccgccc
      661 gcgcccgaat ggatggtcag aatggcattc tgccggtcgt atttgcccga cagaaaggcc
      721 aattcctctt tcttttcaat cttttcggcc gccaagtcaa tatcctcttc gccgccggcc
      781 tcttccagca gatccagctc cgtttccaga tcctccaact ctccgatatt ctttttcaag
      841 tccgccaatt cttcggaaat cgccgcggct tgttcgggaa ctccccaaaa atccggccgc
      901 gcggcaagct catccaactc ctttattttc tcttttttgg cggcaatgtc aaaggcggtc
      961 catcaaaaga tggagcttat cccgcaatcc ccggattttt tcgtttgaat cggccatatt
     1021 cacacttact gtttaccaca taaatttcca aaagtgaagc cgcattgttt ggtcattgat
     1081 atattgataa ctgataattt attatttatc attgccgatt tgccgaaaac tggaaaccga
     1141 ccggccaaaa gccgagaaaa caacccaaag ggttgttttt gcgcatcaat gagccgagaa
     1201 gaggcttcga acctccgacc tgcggtttac gataccgccg ctctgccaac tgagctatct
     1261 cggcgaagaa tagtttgtga gccaatgaaa aaacacggac acaaattcat taacgcaagg
     1321 gaattttagc acaaaatata aaaaagaaaa gaggcgccgg gcggcggcgt ttaaaaaaac
     1381 aggccctgaa aatggcgcgt tgagaggaaa gagtttttaa aaacaacgcg ccattttcgg
     1441 agcctgattg ccttgccata tgtttcagcg gcggtttggg aagtccttca atgaattata
     1501 aaaattaagg actgtgccca aaccgccgct ggaattttcg cttttgaaaa gcccggcaaa
     1561 atgatgaaga cgatgggcgg catccggcgc gatattggta gatatactca ccgggcttaa
     1621 cccgacggac cgcgttggtg gctccagccc aaggaataaa tcccgaaagc cagcggccgc
     1681 gcaatccgta actctttcct cttcatcatt ctgccaagct tgaaatgtac ctttatcctt
     1741 aca
//
LOCUS       DAOQWE010000161         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_82927_length_1741_cov_4.556346, whole genome shotgun sequence.
ACCESSION   DAOQWE010000161 DAOQWE010000000
VERSION     DAOQWE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82927_length_1741_cov_4.556346"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKK37_04345"
     CDS             complement(<1..456)
                     /locus_tag="PKK37_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81640.1"
                     /translation="MKFLAFRQTKLGSSFDITRARVGGASVIPLLSFLLLSVPPSRRK
                     VLGAKPKQAGKKEGGWGEGIFARLLPFPFRLRKEKAEGGFGGNSAFSFRNQIADSPAT
                     CLNTWKAEHRRKILFSLKEEKIGRAQNEKSKEYFSVVWRACRAVAGLASL"
     gene            521..730
                     /locus_tag="PKK37_04350"
     CDS             521..730
                     /locus_tag="PKK37_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81641.1"
                     /translation="MSNEKSTIGDNIKKYRNKLGISQDQLSKKADLAFHTIAKIEAGS
                     TPNPTIDTVKKIADALGVSLDDLMK"
     gene            735..>1741
                     /locus_tag="PKK37_04355"
     CDS             735..>1741
                     /locus_tag="PKK37_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81642.1"
                     /translation="MANTKEAKARIKINKLLEQAGWRFFDNEKGQANISLEPNVKLTQ
                     KDIDAFGEDFESTKNGFIDFLLLDEKGFPLVILEAKKEDKNPLDGKEQARKYAHTENA
                     RFVILSNGNLHYFWDLERGDPEIITEFPTQESLKHRQDFKPDNQRLADEEVNDDYIAL
                     TQNPNFKEDPRYQKEETREQYLWDENLRILRPYQLKAIHALQKSAEAGNDRFLFEMAT
                     GTGKTLISAAVIKLFLRTGNAKRILFLVDRLELEDQAHKNFVRYLSNDFSSVIYKQNR
                     DDWKKAEIVISTVQSLSAQDKYKKLFSPTDFDLIIADESHRAIGGNSRAVFEYFIGYK
                     LG"
BASE COUNT          565 a          383 c          343 g          450 t
ORIGIN      
        1 taaggaagcc agccccgcca ctgctcgaca agctcgccac accacagaaa aatactcttt
       61 gctcttttca ttttgcgcgc gcccgatttt ttcttctttt aatgaaaaga gtatttttct
      121 gcggtgttct gccttccaag tattcaggca ggtggcgggg ctgtccgcaa tttgatttcg
      181 aaaagaaaaa gcggaattcc ccccaaaccc cccttccgct ttttcttttc tcaaacggaa
      241 cgggaacgga agcaggcggg caaaaattcc ttccccccaa cccccttcct ttttgcccgc
      301 ctgcttgggc ttcgccccca aaacttttcg gcgggacggc gggacagaaa gcaggagaaa
      361 ggacaaaaga ggaataacgc ttgccccgcc cacccgtgcg cgcgttatgt caaaggaact
      421 ccctaatttt gtttgacgaa atgctaaaaa tttcatatac taaccaatga taagtaatga
      481 ttttgataat atataactat catagtaaaa ctatacgaat atgtcaaacg aaaaatccac
      541 aatcggggac aatataaaga agtaccgaaa caaactcggt atctcgcaag atcagctttc
      601 aaaaaaggcg gatttggctt ttcatacgat tgcaaaaatc gaggcaggct caacccctaa
      661 cccgacaatt gatactgtca aaaaaattgc ggacgctctc ggcgtttcgc ttgatgattt
      721 aatgaaataa aactatggca aatactaaag aagcaaaagc gcgaataaaa ataaacaaac
      781 tgcttgaaca agcgggctgg cgattttttg ataatgaaaa aggacaagca aatatttcgc
      841 ttgaaccgaa tgtaaaactt acccaaaaag acattgacgc ttttggcgaa gattttgaat
      901 ctacaaaaaa cggctttatt gattttttat tgcttgatga aaaaggtttc ccgctcgtta
      961 ttttggaagc caaaaaagaa gacaaaaatc cgctagacgg aaaagaacaa gcgcgaaaat
     1021 acgcacacac cgaaaatgct cgttttgtaa ttctttcaaa cggcaatctt cattattttt
     1081 gggatttgga acgaggcgat cctgaaatta tcacagaatt tccaacgcaa gaatctttga
     1141 aacacagaca ggatttcaaa ccagacaatc agcgtttggc ggacgaagaa gttaatgatg
     1201 attatatcgc cctcacgcaa aacccgaatt tcaaagaaga tcctcgttat cagaaagaag
     1261 aaactcgcga acaatatctt tgggacgaaa atttgcgaat tttgcgccct tatcagctca
     1321 aagcaattca cgctttgcaa aaatccgccg aagctggaaa tgatcgcttc ctttttgaaa
     1381 tggcgacagg aacaggaaaa actcttattt ccgccgccgt catcaagttg tttttacgaa
     1441 caggcaacgc gaaaagaatt ttgtttttgg ttgatcgttt ggaattggaa gatcaggcac
     1501 acaaaaactt tgtccgctat ttgagcaacg atttttcttc tgttatctac aaacaaaacc
     1561 gcgatgattg gaaaaaagca gaaattgtca tctcaacagt tcaaagtctt tcggcgcaag
     1621 ataaatacaa aaaacttttt tcaccgactg attttgattt gattatcgcc gatgaatcgc
     1681 accgcgcaat cggcggaaac tcgcgcgccg tttttgaata ttttatcggc tacaaattgg
     1741 g
//
LOCUS       DAOQWE010000162         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_83913_length_1729_cov_7.438471, whole genome shotgun sequence.
ACCESSION   DAOQWE010000162 DAOQWE010000000
VERSION     DAOQWE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83913_length_1729_cov_7.438471"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1729)
                     /gene="dnaE"
                     /locus_tag="PKK37_04360"
     CDS             complement(<1..>1729)
                     /gene="dnaE"
                     /locus_tag="PKK37_04360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR81643.1"
                     /translation="DEALAKADDLKTLVQNDEKARVLMGFAKKLEGCARHASTHACGV
                     VISPVPLDEIVPVQHPTADDNGLVSQYELHAIEDMGLLKMDFLGLKNLTIIEDTLARI
                     YLVQNQSIELKAIPMDDKEAYALMARGDTVGVFQLESAGMQRYLKQLQPTQFEDIIAM
                     VALYRPGPMQFIPDFIDGKSGLKIPQYLHPKLEPILGPTYGIILYQEQTMKIAQELAG
                     FSMAEADTLRKAIGKKIKDLMMASKQKFIDGCQKNGIAKKIAVEIWSWIEPSADYSFN
                     KSHAACYAMIAYQTAWLKAHYPAEFMAALITSEKNDVERVGYLIGECKNMGLEVLAPD
                     INESYHGFTVIPESNKIRFGLAAVKNVGENLVNEVVSQRKSGGKFRDIFDFTERINPK
                     VINRKSLEALIKAGVFDAMAERNTLLASVEKLLEIGRENERNKSNGQQGLFAALPAAK
                     INGHSLSSAHPAGKAEKLMWEKELLGLFVTSHPLEEYKDEMEKKCLKISRVKESQAAR
                     MVKIGGMISTVKKIITKSGKPMLFMSLEDLTDKIEVVVFASTMEKNPVVFCESKVVYV
                     YGRIDLRDGE"
BASE COUNT          356 a          475 c          416 g          482 t
ORIGIN      
        1 tttcgccgtc gcgcagatca atgcggccat agacgtaaac caccttgctc tcgcaaaaca
       61 ccaccggatt tttttccatc gtcgacgcga acacgaccac ttcaatttta tcggtcagat
      121 cttccaggct cataaacagc atcggcttgc cgcttttggt gataatcttt ttgacggtgg
      181 aaatcattcc gccgatcttc accattcgcg cggcctggct ttctttcacg cgggaaattt
      241 tcaggcactt tttttccatt tcgtccttgt attcctccag cggatgggaa gtgacaaaca
      301 gccccaaaag ctctttttcc cacatcagtt tttccgcctt gccggcaggg tgcgcggagg
      361 acagcgaatg gccgttgatc ttggccgccg gcagggcggc gaaaagcccc tgctggccgt
      421 tggatttgtt tctttcattt tcccggccga tttccaaaag tttttcaacg ctggccagca
      481 gggtgtttct ttcggccatt gcgtcaaaaa cgccggcctt gatcagcgct tccagagatt
      541 tgcggttaat cactttggga ttgattcttt ccgtaaaatc aaaaatatcc cggaacttac
      601 cgcccgattt tcgttgcgaa accacctcat tgaccaagtt ttcgccgaca tttttcaccg
      661 ccgccagacc gaagcggatt ttgttgcttt cgggaatcac ggtgaacccg tgatagcttt
      721 cattaatgtc gggtgccagc acttccagtc ccatattttt gcactcgccg atcagatagc
      781 ccacccgttc cacgtcgttt ttttcggaag tgatcagcgc ggccataaat tccgccggat
      841 aatgcgcttt caaccaggcg gtttggtagg caatcatcgc gtagcaggcc gcgtgcgact
      901 tattgaaaga atagtccgcg ctgggttcaa tccagctcca aatttcaacg gcgatctttt
      961 tggcgatgcc gtttttttgg caaccgtcaa tgaatttctg ctttgacgcc atcatcaaat
     1021 ctttgatctt tttgccgatg gctttgcgca gggtatcggc ctcggccatg gaaaatccgg
     1081 ccagttcctg cgcgattttc attgtttgtt cttgatacaa aataatgccg taggtcggtc
     1141 ccaggatcgg ctccagcttg ggatgcaaat actgcgggat tttcaagccg cttttgccgt
     1201 caatgaaatc cggaatgaat tgcatcggac cggggcgata gagggcgacc atggcgatga
     1261 tatcttcaaa ttgcgtgggc tgtaattgtt tcaaatatct ttgcatcccc gcgctttcca
     1321 actgaaacac tcccacggtg tcgccgcgcg ccatcagcgc ataagcctct ttgtcgtcca
     1381 tcggaatggc tttaagctca atgctttggt tttgcaccaa atagatccgc gccagcgtgt
     1441 cttcaatgat ggtcaagttt ttcaatccca ggaaatccat cttcaacagc cccatatctt
     1501 caatggcatg cagttcatat tgggaaacca gtccgttgtc gtcggcggtg ggatgttgca
     1561 ccgggacgat ctcgtccagc gggacgggtg agatcaccac gccgcaggcg tgcgtggacg
     1621 cgtggcgggc gcaaccctcc agttttttgg caaagcccat cagcacccgc gctttttcat
     1681 cgttttggac caaagttttc agatcgtcgg cttttgccag cgcctcgtc
//
LOCUS       DAOQWE010000163         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_84037_length_1728_cov_2.472803, whole genome shotgun sequence.
ACCESSION   DAOQWE010000163 DAOQWE010000000
VERSION     DAOQWE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84037_length_1728_cov_2.472803"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PKK37_04365"
     CDS             <1..307
                     /locus_tag="PKK37_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNR81644.1"
                     /translation="IGPSDWVPFLKDNKICFIRMEGRKFGDIPVEIELRLSVEDSPNS
                     AGVVIDALRMVKLAKDRSIGGPLTSPSSYFMKHPPVQFTDSQAYEMVEEFLAGKRDR"
     gene            438..1031
                     /locus_tag="PKK37_04370"
     CDS             438..1031
                     /locus_tag="PKK37_04370"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNR81645.1"
                     /translation="MLDLHRNWFGGIENKTANVFAALPFTPNQYTSLSIASALAMLYP
                     IVAGHYVAALALFIVAAGLDFIDGAVARKRGLATKRGAYWDTIADRYVEAMLLFGLLF
                     CRLPDFYLPVTAWIFLALFGSTMTTYAKAAAKEKELREAELKGGLMSRGERLIAYFLI
                     LAAMIWGNNYLAAAITAILAVLTNITAGQRISAALRR"
     gene            complement(1105..>1728)
                     /locus_tag="PKK37_04375"
     CDS             complement(1105..>1728)
                     /locus_tag="PKK37_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81646.1"
                     /translation="FVTSVTYDDYLVHTPDLQSTMVNADNACQARAAAAGLTAKGEIY
                     KALMYETACKQGTSNCNLINIARRPHDILPAAALWNGERIGTTANCEWHLVATDPGDM
                     FSADASGNYLQNPIEFNELGQPTNVQVFTNFQPTGSGSYNTANTCFASNTCHYYWDGC
                     YYGQSTSKNQNWAGTYSGHWNPGEVGQPSSCPAVCDTVKRALYCVEQ"
BASE COUNT          391 a          406 c          448 g          483 t
ORIGIN      
        1 catcgggccg tcggattggg tgccgttttt aaaggacaat aagatttgct tcatcagaat
       61 ggagggccgg aaattcggcg acattccggt ggaaattgag ttgcgcctgt cggtggagga
      121 ttctccgaat tccgccggcg tggtgattga cgctctccgg atggtcaaat tggccaaaga
      181 tcgcagtatc ggcggtccgc tcaccagtcc gtcgtcctat tttatgaagc atccgccggt
      241 gcagttcacc gattcccaag cctatgaaat ggtggaagaa tttctcgccg gcaaacgcga
      301 ccgctgatta aaaaattttc aattttcaat tttcaatttc caataaatta ttaaatttcc
      361 aatttaaaaa tggacgcgcg atgtcccatt ggaaatcgtt ttggatgctg gaggtttgag
      421 aattgagatt atctgttgtg ttggatttac atcgaaactg gtttggaggc attgaaaaca
      481 aaaccgcgaa tgttttcgcg gctttgccgt tcactcccaa ccaatacact tcgctttcaa
      541 ttgcgtccgc gttagcgatg ctttatccga ttgtcgccgg ccattatgtt gccgccctgg
      601 cgctgtttat tgtggcggcc ggcttggact tcattgacgg cgcggtggcg cgcaaaaggg
      661 gcctggccac gaagcgggga gcctactggg acacgattgc cgaccgctac gtggaagcga
      721 tgttactttt tggattgctg ttttgccgat tgccggactt ttatctgcca gtcaccgcat
      781 ggatatttct ggcgcttttc ggttcaacga tgaccaccta cgccaaagcc gccgccaaag
      841 agaaggaatt gcgcgaggcc gaattaaagg ggggattgat gtcccgcggc gaaaggttga
      901 ttgcttattt tttaatcttg gcggcaatga tttggggcaa taattatttg gccgccgcaa
      961 tcaccgcgat attggctgtg ctgaccaaca tcaccgccgg acaaagaatc tcggcggccc
     1021 ttcgccgata gctttaaccg agattttcaa aaaaagaacc ccacggggtt cttttttttg
     1081 gattcaactg cgaaagcaaa gccattattg ctccacgcaa tagagtgctc tttttactgt
     1141 atcgcagact gcggggcaac tactaggttg accgacttcc ccggggttcc aatggccaga
     1201 gtatgttccg gcccaatttt gatttttgct ggtgctttgt ccgtagtaac atccgtccca
     1261 gtaataatgg caagtattgg atgcaaagca agtattggca gtgttatagg agccgctacc
     1321 ggtgggctga aaattagtga atacttgcac attggttggt tggccgagtt cgttaaattc
     1381 tatcggattt tgcaaataat tgccgctggc atcagccgag aacatatcgc cgggatcggt
     1441 tgctaccaaa tgccattcgc aattcgcggt tgtgccgatt ctttctccgt tccacaatgc
     1501 cgccgctggc agaatatcgt gaggtcggcg cgcaatattt attaggttgc aattacttgt
     1561 tccttgttta caggcagttt catacatcag cgctttgtat atttctccct ttgcggtgag
     1621 tccggccgcc gccgcgcgcg cttggcaggc attatcggca ttaaccattg ttgactggag
     1681 atccggggta tgtacaagat aatcatcata agtcacactg gtcacgaa
//
LOCUS       DAOQWE010000164         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_84224_length_1725_cov_7.000599, whole genome shotgun sequence.
ACCESSION   DAOQWE010000164 DAOQWE010000000
VERSION     DAOQWE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84224_length_1725_cov_7.000599"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..1604)
                     /locus_tag="PKK37_04380"
     CDS             complement(147..1604)
                     /locus_tag="PKK37_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNR81647.1"
                     /translation="MPYPQVFLSGCLAMMAGAGAASMISISRFLFWVLAAVAVFLFFA
                     GLAAKNHKIAVAGILAAAFVLGFWRFDAAWQKTVDNDLAKLNGQTVEVAGTVVRDPVL
                     KDSSQQIILRPDGIEGNIMVLARRYPEYVYGDKIRFIARLEAPQNFSDFDYKNYLAKD
                     GIYAMARWPEIEPVSENNGNPIYAGLLRIKHHLKRGAGESLPAPQNLLLIAILLGDQS
                     GLGEELKEKLNVSGLRHLSAVSGAHVAIMAQVIVPFLIALGWWRQKALWATIVFVWLF
                     VAMIGLPASAVRAAVMGSLAMFAKIIGRPGDTLRIIAIAGAAMVWQNPLILRFDVGFQ
                     LSFLAVLGMVFFARPVEKKIGFVRPKFARQALSVALAAQIFTLPLVVYNFGYVSSYGL
                     LANVLVEPLVSFITIYGFVLAICGAISPVLGWILFFPIWLALSYLLAVNDFFAGLPGA
                     KMEFEVGFFWLALAYAVLAAIAHRIKEREKLAFLQ"
BASE COUNT          487 a          537 c          351 g          350 t
ORIGIN      
        1 cgcgcggcga tttttggttt ccgtttccgt tttcatcttc aaagaaaatc tttttcaaaa
       61 aattttcccg ctacgattac atcataccaa aaaaaatccc gcgcaacaaa aaatctaaaa
      121 acgcaatccg attattgcct gctgatctat tgcaggaaag caagtttttc gcgttccttg
      181 attctgtggg cgattgccgc caaaacggca taggcaagcg ccagccagaa aaaaccgact
      241 tcaaattcca ttttggcgcc gggcaacccg gcgaagaaat cattcaccgc cagcaaatag
      301 gacagtgcca gccaaatggg aaaaaacaga atccatccca gcaccgggct gatcgccccg
      361 caaatcgcca aaacaaatcc gtaaatcgtg ataaacgaca ccagcggctc cactaaaacg
      421 ttggcgagca acccgtaaga cgaaacataa ccgaaattat aaaccaccag cggcagagtg
      481 aaaatctgcg ccgccagcgc caccgataac gcttggcgcg cgaatttcgg acggacaaaa
      541 ccgattttct tctcaaccgg ccgcgcgaaa aacaccattc ccagcaccgc caaaaacgac
      601 aattggaacc cgacatcaaa acgcaagatg agcggatttt gccacaccat cgcggcgccc
      661 gcaatggcga taatccgcaa cgtatcgccg gggcgcccga tgattttggc aaacatcgcc
      721 aaactgccca ttaccgcggc gcgcaccgcc gacgcgggca ggccgatcat cgccacaaac
      781 agccacacaa aaacgattgt cgcccaaagc gccttctgcc gccaccatcc cagcgcgatc
      841 aaaaacggca cgattacctg cgccataatc gccacgtggg cgccggaaac cgccgacaaa
      901 tggcgcaatc cggaaacgtt cagcttctct tttaattctt cccccaatcc gctttgatct
      961 cccagcaaaa tcgcaatcaa cagcaaattt tgcggcgccg gcaggctttc gcccgctccc
     1021 cgcttaagat gatgttttat tcgcaataat cccgcgtaaa tcggattgcc gttgttctcc
     1081 gacaccggct caatctccgg ccagcgcgcc atcgcgtaga ttccgtcctt ggccagataa
     1141 tttttataat caaaatccga aaaattttgc ggcgcctcca atcgggcaat aaatcggatt
     1201 ttatcgccgt aaacatattc cggataacgg cgcgccaata ccataatgtt gccttcaatt
     1261 ccgtcagggc gcaagataat ctgctggctt gaatctttca aaaccggatc gcggacaacc
     1321 gttccggcaa cttcaaccgt ttggccgttt agttttgcca aatcgttgtc aaccgttttt
     1381 tgccacgccg catcaaaccg ccaaaagccc aaaacaaacg ccgccgccaa aattcccgct
     1441 accgcgattt tatgattttt cgccgccaat cccgcaaaaa acaaaaaaac ggcgaccgcc
     1501 gccaaaaccc aaaacaaaaa gcgcgaaatt gaaatcattg acgccgcccc cgccccggcc
     1561 atcatcgcca aacacccgga taaaaacacc tgcggataag gcatattgat aattatcctt
     1621 ttgttttgcc gcagaatagc gcttcgtttt tttgttcttt gtccccgtcg gacagcggcg
     1681 cgccccgcaa aaccgcccaa atcgccagcg ccaccatatg tttgc
//
LOCUS       DAOQWE010000165         1722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_84428_length_1722_cov_6.790642, whole genome shotgun sequence.
ACCESSION   DAOQWE010000165 DAOQWE010000000
VERSION     DAOQWE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84428_length_1722_cov_6.790642"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1722)
                     /locus_tag="PKK37_04385"
     CDS             complement(<1..>1722)
                     /locus_tag="PKK37_04385"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81648.1"
                     /translation="LRVQDSAEADKFVVTNAGRVGIGTTSPAYTLDVNGGFRAGNATS
                     TNALVLDPNTGNVGVGTASPVQKLHVEGHCITGDSELAIVSPPNQEEQIRKIQIKDVQ
                     PGTQVLTLNEQTNRLEARAINKLLDMGVQAVFRLTTSSGKTIRTTGNHPYLAKISSAE
                     QQQRISLREIGAMEREYGIDKNGLSVDREFSQTKTIWAGESEIAATKKPACADSWTSS
                     DSVQISGLSPNAIIADAPILSMAAASRRDFRVVEVGGIEPPNPWVTIQSSHRATPTTS
                     ELYHENDDGAKWTKVIYLSVGDEIATVSLDHRLSNSEPEVSWEKISSIEYVGQEQVYD
                     IEVAGTHNFVANEILAHNTYISGNVGIGTASPAEALDVNGRVRLAQTTAPATVTDKLY
                     NVSGNLLWNGIDLTSGGALPVGTEGQMLYNNAGAWTAFDGLYWDDTTSRLGIGTTAPI
                     YKFNVAGANAGTDVGDASLLGIVNTDTTANNTIGFAFGQANLSGTIQTVAGIDLVGVS
                     HADGAQSGALAFSTRNAGSWSERLRIDNTGNVGIGTTAPSGLLHVAGGQCVTGDTLLR
                     VRRRRKR"
BASE COUNT          348 a          523 c          440 g          411 t
ORIGIN      
        1 tgccgcttgc gccgccgccg cacgcgcaaa agcgtgtcgc cggtaacgca ctgcccgccg
       61 gccacatgca acaaccccga cggcgcggtg gtcccaattc ccacattacc agtgttatca
      121 atgcgcaaac gctcgctcca actgcccgcg ttcctcgttg aaaacgccag cgcgccgctc
      181 tgcgcgccat cggcatggct cacacccacc aaatcaatcc cggccacggt ttgaatcgtg
      241 ccgctcaaat tcgcctgccc gaaagcaaaa ccgatggtgt tgttggcggt ggtgtcggtg
      301 ttcacgattc ccagcaaact cgcatccccc acatccgtgc cggcattcgc ccccgcaaca
      361 ttaaacttgt aaatcggtgc agtcgtccca atccccaagc gcgaggtggt gtcgtcccag
      421 tataatccat caaacgcggt ccacgcgccg gcgttgttgt agagcatttg gccttcggtg
      481 cccaccggca gagcgccgcc ggaggtgagg tcaatgccat tccacaacaa attgcccgac
      541 acattgtaga gcttgtccgt cactgtcgcc ggcgcagtgg tctgcgccag ccgcacccgc
      601 ccgttgacat ccagcgcctc cgccggactc gccgtcccga tgccaacatt gccggagatg
      661 taagtgttgt gggctaaaat ttcattggcg acaaagttgt gggtgccggc cacttcaatg
      721 tcatagactt gttcttggcc aacatattca atggatgaaa ttttttccca cgatacttcc
      781 ggttcggaat ttgaaagtct gtgatccaaa gaaacagtcg caatctcatc gccgaccgac
      841 aaataaatca ctttggtcca ttttgcgcca tcatcattct cgtggtataa ttcggatgtc
      901 gtgggggtag ctcggtgcga gctctggatt gtcacccaag ggttcggggg ttcaattccc
      961 cctacctcca cgacacgaaa atcacgccgg cttgccgccg ccattgacaa aatcggagca
     1021 tccgctataa tggcattggg tgacaatcca gaaatttgta ccgagtcaga actcgtccaa
     1081 gaatccgcgc aagcgggttt tttggttgcc gcaatctccg attcgccggc ccatattgtt
     1141 tttgtttggg aaaactcacg gtcaaccgat aaaccattct tgtcaatgcc atattctctt
     1201 tccatggcgc cgatttcccg cagtgaaatt ctttgttgct gttccgcgga tgatattttt
     1261 gccaaatagg gatgattgcc ggtggtgcgg atggttttgc cgcttgaagt ggtgagccga
     1321 aacaccgcct gcacccccat atccagcagt ttgttaatgg cgcgcgcttc caatcggttg
     1381 gtttgctcgt tcaaggttaa aacttgcgtt ccgggttgaa catccttgat ttgtattttc
     1441 cggatttgct cttcttgatt tggagggctc acaatcgcca actccgaatc gccggtgatg
     1501 caatgcccct ccacatgaag cttctgcacc ggcgacgccg tccccacccc cacattgccg
     1561 gtgttcgggt caaggacgag ggcattcgtt gaagtcgcat tgcccgcccg gaacccgccg
     1621 ttcacatcca gcgtataggc cgggctcgtt gtcccgatgc cgacgcgccc ggcgttggtg
     1681 acgacgaatt tgtcggcttc tgcggagtct tgcacgcgca gg
//
LOCUS       DAOQWE010000166         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_84710_length_1719_cov_2.493990, whole genome shotgun sequence.
ACCESSION   DAOQWE010000166 DAOQWE010000000
VERSION     DAOQWE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84710_length_1719_cov_2.493990"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..>1719
                     /locus_tag="PKK37_04390"
     CDS             216..>1719
                     /locus_tag="PKK37_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNR81649.1"
                     /translation="MKTTKLFKVLFITFFSIYFLKYAPVYADTNIKTLDLTAKTTVKD
                     PYCKGVVYSFNLPAGGVNFRGMSGYVSMTPTEPIFTQTLIWAANLPNGDCPPSGSLLN
                     REDGSTIYPQTDGAPWFAAYILKNSENKYVRLNTEFMLPKPIPMKGCVIVIVGGCGAV
                     ATEITSESHMKMLYDTEPFDKLSKPSLVPLSYEYGYGGGGGASGGANCSGSQCAFASV
                     WKADKDYDLHFFHGGVAGSGFSPVADPPQGNWDADFDYYLYKNCDNLKQGINGPSDYY
                     REIPADATPLFNLRQNVINNAPQTSIKDKISGFGGTGDLPVVGDWNGDGKTEIGNYKG
                     NSVWVLDYDGSYVWDGASVDKIFSFGSSTDKPITGDWNGDGKTEIGNYKGNGMFALDQ
                     NGNGTWDGASVDKVFNFAIANPSSSDLPVVGDWNGDGKDEIGIYQPITKKWYLDYNSN
                     GVWDAGNDKTYQFGGQGDLPVVGDWNGDGKTEIGNYKKNGNWALDDNGNGN"
BASE COUNT          483 a          355 c          438 g          443 t
ORIGIN      
        1 tccgcaaatt gacgcgcgcg ggcgccgcgc gatcgcgcaa gtcccgaacg gtccgggcgg
       61 tgttttcttt ttgataaatg gttataatgc aataattagt tcaattaatc gccgaactgc
      121 aaaaacagtt ggcggcaatg aagtgatttt tttgcgcgcg taatgattat aattttgggg
      181 cgcaataaaa taataaaaat aatcgtaatt aaaaaatgaa aacaacgaaa ttattcaaag
      241 ttttatttat aacatttttt tcaatttatt ttttgaaata tgcgccggtt tatgccgata
      301 ccaatataaa aacattggat ttgaccgcca agaccacagt taaagatcct tactgtaagg
      361 gagtggttta ttcgtttaat ttgcccgcgg gcggcgtaaa tttccgcggg atgtcgggct
      421 atgtatcgat gacgcccacg gaaccgatat ttacccaaac attgatttgg gcggcaaatc
      481 ttcccaatgg ggattgccct cccagtgggt cgctccttaa tcgcgaggat ggatcgacaa
      541 tttacccgca aacagatgga gcgccatggt ttgcggcata tattcttaaa aattctgaaa
      601 ataaatatgt ccggcttaat acggagttta tgcttccgaa gccgattccg atgaagggct
      661 gcgttattgt tatcgtcggc ggttgtggtg cagttgcaac cgaaattaca tcggaaagcc
      721 atatgaaaat gctgtatgac accgaaccgt ttgataaact aagcaaacct tcgcttgttc
      781 cgttaagcta tgaatacggt tatggcggtg gaggcggcgc gtccggaggg gcaaattgct
      841 cgggaagcca atgtgctttt gccagcgttt ggaaagcgga taaggattat gatttgcatt
      901 tttttcatgg cggtgttgcc ggcagcggtt ttagtcccgt tgcggatccg ccgcagggga
      961 attgggatgc tgattttgat tactatcttt ataaaaattg cgataattta aaacaaggca
     1021 tcaacggacc gtccgattat taccgcgaaa ttccggcaga tgccacgcca ctttttaacc
     1081 ttcgccaaaa tgtaatcaat aatgcacctc aaacatcgat aaaagataaa atatccggct
     1141 ttggcggcac gggcgacttg cccgttgtgg gcgattggaa cggcgacggc aagactgaaa
     1201 tcggcaacta caaaggcaac agcgtttggg tattggatta tgatggtagt tatgtttggg
     1261 acggcgcatc tgttgataaa atttttagtt ttggatcgtc aaccgataag cccatcaccg
     1321 gagattggaa cggcgacggc aagactgaga tcggcaacta caaaggcaac gggatgttcg
     1381 ctttggatca aaacggcaat ggcacatggg acggcgcatc tgttgacaaa gtttttaatt
     1441 tcgcaatcgc caatccatct tccagcgatc tgcccgttgt gggcgattgg aacggcgacg
     1501 gcaaagatga aatcggcatc taccagccca taaccaaaaa atggtatctg gattacaaca
     1561 gcaacggcgt ttgggatgcg ggaaacgaca aaacatacca attcggcggc cagggcgatc
     1621 tgcccgttgt gggcgattgg aacggcgacg gcaagaccga gatcggcaac tacaaaaaaa
     1681 acggtaactg ggcattggat gataatggca acggcaatt
//
LOCUS       DAOQWE010000167         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_85227_length_1712_cov_3.647556, whole genome shotgun sequence.
ACCESSION   DAOQWE010000167 DAOQWE010000000
VERSION     DAOQWE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85227_length_1712_cov_3.647556"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKK37_04395"
     CDS             <1..329
                     /locus_tag="PKK37_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81650.1"
                     /translation="AIPEISRGGMALSDPIMIWEPVGCSKCKRQGFSGRVGIFETLEM
                     TAELGEIVLKNLSDSALGAEARRQGLITMRQDGVLKALKGMTTVEEVLKATEEDISAA
                     VSGKKP"
     gene            518..955
                     /locus_tag="PKK37_04400"
     CDS             518..955
                     /locus_tag="PKK37_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR81651.1"
                     /translation="MVTTEKSTAAGGKKILVVEDDPFLSDIYHTKLKQAGFAVDLAMT
                     GEECMEKLLGGEYDLMVLDIVLPQLDGWEILARVGKMRREKPDSYINRLKIIILSNLG
                     QKEEIKKGLDLGADGFMIKAHFTPSEVAAEINKALETPKAMNG"
     gene            1057..>1712
                     /locus_tag="PKK37_04405"
     CDS             1057..>1712
                     /locus_tag="PKK37_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR81652.1"
                     /translation="MDYSESLKKYLATTVEKNASDLHLTAGYPPIIRINGELWAIPKE
                     APLSARDSQGLAFALMTPEQQNQFLKFKEIDFSYAFEGKERFRTNVFFQRGCVSCALR
                     LIPTKIRTVSELNLPPIMQKMIDSEQGFVLITGPTSHGKSTTLAALVDAINHTRCDHI
                     VTIEDPIEYLFTPDCSVIEQRELNSDTYSFSMALRSVFRQDPDVIMIGEMRDAETIST
                     A"
BASE COUNT          469 a          372 c          461 g          410 t
ORIGIN      
        1 acgcgattcc cgaaatttcg cgaggaggaa tggcgctttc ggatccgata atgatttggg
       61 agccggtcgg ctgttcaaag tgcaaacggc aggggttttc cggccgggtg gggatttttg
      121 aaacgctgga aatgacggcg gaactgggag agattgtttt gaaaaatctt tccgactccg
      181 ccttgggggc cgaggcgcgg cggcaagggt tgatcacgat gcgccaggac ggggttttga
      241 aagcgttaaa aggaatgacc acggtggaag aagttttgaa agccactgaa gaggatattt
      301 ccgcggcggt gagcggcaaa aaaccgtaag ggtctatccc tcgctaatgc gagggttaga
      361 ccctggcaaa agaattttgg gcgcaaaatt aaaggggact aacccccgca gtagcgaggg
      421 atattcccct ttaatttacc cccagtgagt ggttgcacga cgtcgccttt gcttgacaaa
      481 aaaaataggt tataatgact caataacatt actgattatg gtaacaactg aaaaaagcac
      541 tgccgccggc gggaaaaaaa ttctggtggt ggaagacgat ccgttcttgt ccgacattta
      601 tcataccaaa ttgaagcagg cgggttttgc cgttgatctg gcgatgaccg gggaagagtg
      661 catggaaaag ttgttgggcg gcgaatatga cctgatggtg ctggatattg ttttgcccca
      721 attggacggg tgggaaatat tggcgcgcgt cggcaaaatg cgccgcgaaa aaccggattc
      781 atacatcaat cgcttgaaaa tcatcatcct ttccaatctg gggcaaaaag aagagataaa
      841 aaaggggctt gatttgggtg ccgacgggtt tatgatcaag gcgcatttca ctcccagcga
      901 ggtggcggcg gaaattaata aggcgctgga aactccaaaa gcaatgaacg gttagtttaa
      961 ttaaaataaa aattcaaaat tgaaaattgt gaaatgcggc gtttgcgcgt ttcaaaccgg
     1021 ttttaaattt ttcaatttta attttcaatt tgaaatatgg attattcaga atccttaaaa
     1081 aaatatttgg cgaccaccgt ggaaaaaaac gcctccgatc tccatctgac ggccggctat
     1141 ccgccgatca tccgcatcaa cggtgagttg tgggcgattc ccaaagaggc gccgctttcg
     1201 gcgcgggaca gccaggggtt ggcgttcgcc ttgatgactc ccgaacaaca gaaccagttt
     1261 ttgaaattca aagaaattga tttttcttac gcgtttgaag gcaaggaaag attccggacc
     1321 aacgtttttt tccagcgggg gtgcgtttcc tgcgccttgc ggctgattcc caccaaaatc
     1381 agaacggtaa gcgagctcaa tttgccgccg ataatgcaaa aaatgattga ttccgagcag
     1441 gggtttgtgt taatcaccgg accgacatcg cacggaaaat caaccacgct ggccgcgttg
     1501 gtggacgcaa tcaaccacac ccgttgcgat cacatcgtta ccattgaaga cccgattgaa
     1561 tatctgttca cacccgactg ttcggtgatt gaacaaagag aattgaattc cgacacctat
     1621 tccttttcca tggcgttgcg atcggttttc cgccaggatc cggacgtgat tatgatcggg
     1681 gaaatgcgcg atgccgaaac catcagcacc gc
//
LOCUS       DAOQWE010000168         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_86665_length_1694_cov_3.726052, whole genome shotgun sequence.
ACCESSION   DAOQWE010000168 DAOQWE010000000
VERSION     DAOQWE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86665_length_1694_cov_3.726052"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1694)
                     /locus_tag="PKK37_04410"
     CDS             complement(54..>1694)
                     /locus_tag="PKK37_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81653.1"
                     /translation="EITDDEADSQEVVLQTAYKKRLELKKLFEKENANINPLVLVQIP
                     TAEAGEDKIKAVRKFLADKGITERKDGTGNGKLAIWLSEQKSETLDWVSEPDNEIEFL
                     IFKQAIDTGWDCPRAHILVKFRESHSETFEIQTVGRILRMPEQKHYASEDLNRGYIYT
                     NVQSIIVKKEEYNPNIIKHLKAIRKEVYKPIKLTSYYKSRADYGDITSSFTPVFEKTA
                     CEHFGLKGDHTMFAQNVAKVEKEGVILDVKKYQQDIIANAKIEGMTFDEIEGKIDSAA
                     RARLTIAGNDLQALFEQIIKERLGSFKNVKRSVPAVKTAIYTWFRKYLGSKAWPEEMI
                     LVQMVLAHNGNRKKFEEILASAIEAYKAVREKEILKRVEESEQFYDFEIAKESFFNQH
                     TDERVEHEKFVYEPCYLSASRLNPEKNFEKFLAENSDKIVWWWKNGENKQDYFGVKYE
                     YPAGVIHTFYPDYLVQLADGRIGIIETKDMGDRDGGNYTKAKAEKLQEYIKEQKGKKL
                     FGGIAIEKSDGWKINQKSVYDWSKCEKNDWSEWEKLKF"
BASE COUNT          416 a          412 c          280 g          586 t
ORIGIN      
        1 acctttcctt tttcgcttcc gaatttcgtg ccagttgaaa actggcgcgc cacctagaat
       61 tttaattttt cccactcact ccaatcgttc ttttcgcact tgctccaatc atacaccgat
      121 ttttgattta ttttccagcc gtcgcttttt tcaatcgcaa taccgccgaa aagttttttg
      181 cctttttgct ccttgatata ttcttgcaat ttttccgctt tggcttttgt atagtttccg
      241 ccgtcgcgat ctcccatatc tttcgtttca ataattccaa ttcgtccgtc agctaattgc
      301 acgagataat cggggtagaa agtatgaatc acgccggctg gatattcgta tttaacacca
      361 aaataatcct gtttgttttc gccgtttttc caccaccaaa ctattttgtc gctattttcc
      421 gccaaaaact tttcaaaatt cttttcggga ttaaggcgag aagcggacaa atagcacggc
      481 tcataaacaa atttttcatg ctccacgcgc tcatcggtgt gttgattgaa aaagctctct
      541 ttggcgattt caaaatcata aaattgctca ctttcttcca cgcgtttcaa aatttctttt
      601 tcgcgaaccg ccttgtatgc ttcaatcgcg ctcgccaaaa tttcttcaaa ctttttgcga
      661 tttccgttgt gcgccaaaac catttgcacg agtatcattt cctctggcca tgcttttgat
      721 ccaagatatt ttctaaacca agtataaatt gccgtcttca ccgcaggcac agaccttttg
      781 acattcttaa atgagccgag gcgctctttg ataatctgtt caaacaatgc ttgcaaatcg
      841 ttacccgcaa tcgtcaaacg agcgcgcgcg gcggaatcaa ttttgccttc aatctcgtca
      901 aaagtcatgc cttcaatttt ggcgttggcg attatatctt gctgatattt ttttacatct
      961 aaaatcacgc cttccttttc cactttcgcc acattttggg caaacattgt atggtcgcct
     1021 ttcaatccaa aatgttcgca cgctgttttt tcaaacacag gcgtaaaact ggaagtaatg
     1081 tcgccataat ccgcacgcga tttatagtat gaagtcagtt taatcggttt atacacttct
     1141 ttgcggatag ctttcaaatg tttgataatg ttcggattgt attcttcttt tttgacaata
     1201 atgctttgga cattggtata aatatatccg cgattcaagt cctcgctcgc ataatgtttt
     1261 tgttcaggca tacgcaaaat acgcccgaca gtttggattt caaaagtttc gctatgcgat
     1321 tcgcggaatt taaccaaaat atgtgcgcgc ggacaatccc aaccagtatc aatcgcttgc
     1381 ttgaaaatca aaaattcaat ttcattatct ggctcggaca cccaatccaa agtttccgat
     1441 ttctgttccg ataaccaaat ggcgagttta ccgtttccag ttccgtcttt tcgttcggtt
     1501 atgcctttat ccgctaaaaa ctttctcacc gcttttattt tatcctcgcc agcttcggcg
     1561 gtcggaatct gcaccaaaac aagcggattg atattcgcat tttctttttc aaaaagtttt
     1621 ttcaattcca agcgtttttt atacgccgtt tgcaaaacta cttcttgcga atctgcctcg
     1681 tcatcagtta tctc
//
LOCUS       DAOQWE010000169         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_87847_length_1681_cov_2.154367, whole genome shotgun sequence.
ACCESSION   DAOQWE010000169 DAOQWE010000000
VERSION     DAOQWE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87847_length_1681_cov_2.154367"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKK37_04415"
     CDS             <1..479
                     /locus_tag="PKK37_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81654.1"
                     /translation="LMELDASDPEFQSKLARYTSQIQDDSAVLDRQYKQAQIAKIYGD
                     MAGTPERGSSNDDYSRALQFVNDNPDATPQELEQNIRANTNLSEADIKSLVKTKSTLT
                     RETISKMFKIEDNDNKSGFLGLWGETNKEKLDNIMNAITQYQNVGYNDQEILKMMQ"
     gene            481..>1681
                     /locus_tag="PKK37_04420"
     CDS             481..>1681
                     /locus_tag="PKK37_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81655.1"
                     /translation="MALNLSAFQKNIQPKKKTNLNLSAFGITQEKTQQPAQPQSIWRN
                     IPTATDWRKTLPMSIAKPQEQPKQNLLQSAVSGIENIGKGVSEFKSSFGEGWNRGVLS
                     TGSVIERVASLGARNVGLQDISQKLDTLAEKDKELATYGIDVVDTRKFTEKIKDPNFI
                     AKGIGQNLPNMLVAMGAAAPAAIAGAPTAVVGGIGFLTSGMLEGGFAYEDALSMGVDK
                     KKAEKIAGVVGVINGLLETLPITKLLTRSKAGTNIKRSLIREITKRTVAQASMESGTE
                     SIQEIVSNSLATIYDKNRDILSGVPESALFGGIMGGGMSTAMDVAGRVKNVGLTIEDV
                     SGQESEAEARKFPTAEEFLKAQKETIIVHSEEKMGGKLEYKDGLLKYSIDENSGNLII
                     DSIEVKNK"
BASE COUNT          564 a          287 c          422 g          408 t
ORIGIN      
        1 tgttgatgga gctggacgcc agcgatcccg agtttcaatc caagctggca cgatatactt
       61 cccaaataca ggatgatagc gccgtgctgg ataggcaata caagcaggca cagattgcca
      121 agatttacgg ggatatggcg ggaacacccg aacgaggtag ttcaaacgat gattattcaa
      181 gggcattaca atttgttaat gataacccag acgctacccc gcaagaattg gagcagaata
      241 taagagcgaa caccaatttg agcgaagcgg atattaagtc gttggtaaaa acaaaatcaa
      301 ctctaacaag agaaacaatc tcaaaaatgt ttaaaattga agataatgac aataaaagtg
      361 gatttcttgg attatgggga gaaactaaca aagaaaaact tgacaatata atgaatgcga
      421 ttactcaata ccaaaatgtt ggatacaacg accaagaaat tttaaaaatg atgcaatagt
      481 atggcattaa atttatccgc ttttcaaaaa aacatacaac ccaaaaaaaa gacaaatctt
      541 aatttgtccg cttttggtat tacgcaagaa aaaacccaac aacccgcaca acctcaatct
      601 atttggcgca acattcccac tgctaccgat tggagaaaaa ccttgccgat gagtatcgcc
      661 aaaccgcagg aacagccgaa gcaaaaccta ttgcaatcgg ctgtttcggg tattgaaaac
      721 attggcaagg gagtatcaga atttaaaagc agttttgggg aaggatggaa taggggcgtg
      781 ttatctaccg gctctgtaat tgaaagagtt gcgtctttgg gcgcaaggaa tgttggattg
      841 caagacatat cccaaaaact tgacactctt gccgagaaag ataaagagtt ggcaacctat
      901 ggaattgatg ttgttgatac aagaaaattc actgaaaaga taaaagaccc taattttatt
      961 gccaaaggaa tagggcagaa tttaccgaat atgcttgttg ctatgggagc ggccgcgccg
     1021 gcggcgattg ctggcgcgcc tactgccgtt gtgggcggaa ttggcttttt gacttcgggg
     1081 atgctggaag gcgggtttgc ttatgaggac gcgttgagta tgggagttga taaaaagaaa
     1141 gcggaaaaga ttgccggagt tgtgggagtt atcaatggac tgcttgaaac actgccaatt
     1201 accaaattgt tgaccagaag caaggcagga acgaatatca agcgttcgct tattcgcgag
     1261 attacaaaaa ggacggtggc gcaagcgtca atggagtccg gcacggaaag cattcaagaa
     1321 attgtcagca attctttggc aacgatttat gataaaaaca gggatatatt gtccggtgtt
     1381 cccgagtcgg ctttgtttgg tgggattatg ggcggaggta tgagcacggc gatggatgtt
     1441 gccggtagag ttaagaatgt tggtttgact attgaggatg tgagcgggca agaatcggaa
     1501 gccgaagccc gcaaattccc aacggcggag gagtttttga aggcgcaaaa agaaactatt
     1561 attgtccatt cggaagaaaa aatgggtggt aaattggagt ataaagatgg tcttttaaaa
     1621 tatagtattg atgaaaatag tggtaattta attattgata gcattgaggt taaaaataaa
     1681 a
//
LOCUS       DAOQWE010000170         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_88210_length_1676_cov_4.027144, whole genome shotgun sequence.
ACCESSION   DAOQWE010000170 DAOQWE010000000
VERSION     DAOQWE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88210_length_1676_cov_4.027144"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1202
                     /locus_tag="PKK37_04425"
     CDS             114..1202
                     /locus_tag="PKK37_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNR81656.1"
                     /translation="MNARLGKLQKFIAEQELDAIIITSPANIFYLTGIDNFDSEKGFL
                     LIVYKDKWRLVSSIFYKSRIENAIAAKNQLWLKRGDSIAAFAAKQLANCRAIGFERED
                     LSFARYEIFKKSLRGKKLIPISGAAEQFRQIKDESEIKLIKKAARITDKNFAALLKLI
                     KPGITELFLKRKILEMMQDMGAQKCSFDPIVASGKNSADPHYEGSNKKIKAGEMVVID
                     IGARYKNYDADMTRMVFVGKAPTKYKNLYSLVLQTQQKAIAECKAGALPVQIFENAVN
                     NFAAAGEDEFFTHGLGHGVGIDIHELPNLSPAGIGKFDNGMVFTVEPGLYHPGWGGIR
                     IEDLCRMKNGKAEILSRTPKNLIEIQVK"
     gene            1199..>1676
                     /locus_tag="PKK37_04430"
     CDS             1199..>1676
                     /locus_tag="PKK37_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81657.1"
                     /translation="MNKIKKTHLLILIVAITGLIVAGIWESQRFSKLPPAAQENENPA
                     ATTTAENGQNATSSIATFEECVSAGKEVVGEKPNRRCIVRDDLVYIEIETCAAPDGKI
                     MSIFEALNLFNNGKCAWEGSAKDEHFCNETAGAWEIGILAYRKNCAAVCVIDIATKE"
BASE COUNT          493 a          367 c          396 g          420 t
ORIGIN      
        1 aaacagcaaa ttattttctt ttttgccttt ttcttcttcc ttatccattc tttttgtgtt
       61 agtatttttt tgttcgttgc ctttaatcta atctattttt atcaaaaagt aaaatgaatg
      121 ctcgtcttgg aaaactgcaa aaatttatcg ccgagcaaga gctggacgcg ataattatca
      181 ccagcccggc caatatcttt tatttgaccg gcattgacaa ttttgattcc gaaaaaggat
      241 ttttgctgat tgtttataaa gacaaatggc ggctggtttc atcaattttt tacaaaagcc
      301 ggattgaaaa cgcgattgcc gcgaaaaatc agctttggtt gaagcgcggc gattcaatcg
      361 cggcatttgc cgccaaacaa ctggcaaatt gccgcgccat cgggtttgaa cgggaagatt
      421 tgtcgtttgc ccgttatgaa attttcaaaa aatcactgcg tggcaaaaaa ttaatcccga
      481 tatcaggcgc ggcggaacaa ttccgccaaa tcaaagatga gtcggaaata aagctcatca
      541 agaaagccgc gcggatcacc gacaaaaatt tcgccgcgtt gctaaaattg atcaagcccg
      601 gaataacgga attgtttttg aagcgaaaaa ttttggaaat gatgcaagat atgggcgcgc
      661 agaaatgttc ttttgacccg attgtcgctt cgggaaaaaa ttccgccgat ccgcactacg
      721 aggggtcaaa caagaagata aaggcagggg agatggtagt gattgatatc ggggcacggt
      781 ataaaaatta tgacgcggat atgacccgaa tggtttttgt gggcaaggcg ccgacaaaat
      841 acaagaatct ttacagcctc gttttacaaa cccagcaaaa agcgattgcc gaatgcaagg
      901 caggcgcatt acccgtgcag atttttgaaa acgccgtcaa taattttgcc gccgccggag
      961 aagatgaatt tttcacccat ggcttgggcc atggcgtggg aattgatatc cacgaattgc
     1021 ccaatttgag tcccgcggga atcggaaaat ttgataatgg tatggtattt actgtggagc
     1081 ccggacttta ccaccccggc tggggcggga tccgcattga agacttgtgc cggatgaaaa
     1141 acggcaaggc ggaaattctg agccgaacgc cgaagaattt aattgaaatt caagtaaaat
     1201 gaacaaaatt aaaaaaacgc atttgctgat tttaatcgtc gcaattaccg gactgattgt
     1261 tgccgggatt tgggaatcgc aacggttttc aaaattaccg cccgccgcac aggaaaacga
     1321 aaatccggcg gcgacaacca ccgccgaaaa cggccaaaac gccacctcgt ccattgccac
     1381 ctttgaagaa tgcgtttctg ccggcaagga agtggtcggc gaaaaaccca atcgccgctg
     1441 tatcgtccgc gatgatttgg tgtatattga aattgaaacc tgcgccgcgc cggacggcaa
     1501 aataatgagc atctttgaag ccctcaatct tttcaacaat ggcaaatgcg cttgggaagg
     1561 cagtgccaag gacgaacatt tctgcaatga aaccgccggt gcgtgggaaa tcggcattct
     1621 agcctaccgc aaaaattgcg ccgccgtttg cgtgattgac atcgccacca aagaat
//
LOCUS       DAOQWE010000171         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_88681_length_1671_cov_6.079208, whole genome shotgun sequence.
ACCESSION   DAOQWE010000171 DAOQWE010000000
VERSION     DAOQWE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88681_length_1671_cov_6.079208"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1671
                     /locus_tag="PKK37_04435"
     CDS             <1..>1671
                     /locus_tag="PKK37_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR81658.1"
                     /translation="QLAKVYFGSEKNMIRLNMSEYQTIESMEKLIGGAEGPGVLTTAV
                     RDNPFSMVLIDEIEKAHSGLLNIFLNIFDEGEMSDGMGRTIDFKHTIIIATSNAGADY
                     IKEAVDRGSSLANFKDTFVDNILRKNLFKPEFLNRFDALVLYRPLDAAEMRQVAGLML
                     KDIQSGLREKRIELEITDELMEKIGRLGFDPSFGGRAMRRAVQDKIENPLAKALLSGK
                     IKSGDTCEFDCDTWEILTGNERTAYHERKKNHPVKEDEEDPLAPLLMNLEERIHEGLI
                     NQKEAVAELANALRRAHANLKESKRTIGNFLFLGPTGVGKTETAKQLAKVYFGSEKNM
                     IRLNMAEYQNVNSLDKLIGNLQSPGLLTTQVVERPQSLILIDEIEKASPQILNLFLSV
                     FDDGQLADGNGRMVDFRSTINIATSNAGAGIIKEAIEKEVPLDGEFKKSFIDRLLQGG
                     IFTPEFINRFDAVALYRSLNAEENKQVAGLMLKDIQKGLAAKGIVFQITDDLIGALVE
                     LGFDPVFGGRAMRRAVQDKVENAIAKALLSREIRRGDTIRIDPKTWETA"
BASE COUNT          452 a          348 c          490 g          381 t
ORIGIN      
        1 acagctggcc aaggtctatt tcggttcgga aaagaatatg atccgcttga atatgtccga
       61 ataccagacg atagagtcaa tggaaaaatt gatcggcggc gcggaaggac cgggggtttt
      121 gaccaccgcg gtgcgcgaca atcctttctc catggttttg attgatgaga ttgaaaaagc
      181 ccactccgga ttgctgaaca tatttttgaa tatttttgac gaaggggaaa tgagcgacgg
      241 catgggaaga acgatagatt tcaaacacac gatcattatc gccacttcca acgccggcgc
      301 cgattacatc aaagaagcgg tggatcgcgg aagcagtttg gcgaatttca aggatacttt
      361 tgttgacaat attctgcgga aaaatctttt taaaccggaa tttttaaacc ggtttgacgc
      421 actggtgctt tatcgcccgc ttgacgcggc ggaaatgcgg caagtggcgg gcttgatgct
      481 caaagacatt caaagcggat tgcgggaaaa gaggattgaa ttggaaatta ccgacgaatt
      541 gatggaaaag atcggtcggc ttggttttga tccgtcattt ggcggtcggg cgatgcgccg
      601 cgcggtgcag gacaaaatag aaaatccgct ggcaaaggcg ttgttgtcgg gaaaaatcaa
      661 gtcgggggac acttgcgaat ttgattgcga cacttgggag atattaacgg gaaacgaacg
      721 gacggcttat catgagcgca agaagaatca tccggtgaaa gaagacgaag aagatccgct
      781 ggcgccgctg ttgatgaatt tggaagagcg cattcatgaa ggattgatca atcagaaaga
      841 agcggtggcc gagttggcca acgccttgcg ccgggcgcac gcaaatttga aggagagcaa
      901 gcgcaccatc ggcaatttcc tgttcctggg ccccacgggg gtgggcaaga ccgaaaccgc
      961 caaacagctg gccaaggtct atttcggttc ggaaaagaat atgatccgct tgaatatggc
     1021 cgaataccaa aatgtcaatt ctttggacaa actgatcggc aatttgcagt cgccgggatt
     1081 actgaccacg caagttgttg aacggccgca atcgctgatt ttgattgacg aaatagaaaa
     1141 agccagcccg cagatattga atttgttttt aagcgtgttt gacgatggcc agctggccga
     1201 cggcaacggc cggatggttg atttcagaag cacgattaac attgccactt ccaacgccgg
     1261 cgccggaatc attaaagagg cgattgaaaa agaggtgccg ctggacggtg aattcaagaa
     1321 gtcatttatt gaccgccttt tgcagggggg gatatttacc cctgaattta tcaaccggtt
     1381 tgacgcggtc gcgctctatc gctcgctgaa tgccgaggag aacaagcagg tggcagggtt
     1441 gatgcttaaa gacatccaaa aagggttggc ggcaaaaggg attgtttttc aaattaccga
     1501 tgatctgatc ggtgcgttgg ttgaactcgg ttttgatccg gtgtttggcg gtcgggcgat
     1561 gcgccgcgcg gtgcaggata aggtggaaaa cgcgattgcc aaagcgttgt tgtcgcggga
     1621 gattcggcgc ggcgacacga tccgaattga tccgaaaaca tgggaaacgg c
//
LOCUS       DAOQWE010000172         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_89358_length_1663_cov_7.484453, whole genome shotgun sequence.
ACCESSION   DAOQWE010000172 DAOQWE010000000
VERSION     DAOQWE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89358_length_1663_cov_7.484453"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..330
                     /locus_tag="PKK37_04440"
     CDS             46..330
                     /locus_tag="PKK37_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81659.1"
                     /translation="MAETIGEQLENLSEKETKIMALLDEIETNAKSLSQIEKNRAEEM
                     FDDLKFDNEDNVAGWPEELKERYGEVEKIIGDLEENDRINQKEAMGEAAA"
     gene            483..>1663
                     /locus_tag="PKK37_04445"
     CDS             483..>1663
                     /locus_tag="PKK37_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81660.1"
                     /translation="MEEFANNDFWRLLDESYYGLGRAAAPLEKTATLPAFLPWAILGG
                     VLLAGLLAGAWFILKKRNREKFRLSMDMILLSVRISPKNAEEIQQSGRQEKDWIRVME
                     DFYANLVSLSPRGFLAPKPWVALEVAKVKGEICFYAAAPRRYVSFLEKRINSIYPDAQ
                     IEKSNDFNIFGPKESVFCGFAKTIRPVFLPLKTYESIGTDPLSAITNTLTKLEENEEA
                     AVQIVLQNVSEMWRVRGREILDKIKKGKKFSAAMEETGLMGAARGGAKENEPQSFMGN
                     QGDEELVKAMESKLNKRNLAANIRIVVSIKDEFRSEQIFDQLAGSLDQFSSPGLNGFR
                     VVKKKGKSAAAILNDYIFRNFNPANKSILSAEELAGIFHFPTPYLKTPNIKTINAKTA
                     PP"
BASE COUNT          495 a          371 c          438 g          359 t
ORIGIN      
        1 aaaataatca attgccgaaa gccaataact tttggcaaaa atcacatggc ggaaacaata
       61 ggtgaacagc tggaaaattt atccgaaaaa gaaacaaaaa ttatggcgtt gttggatgaa
      121 atagagacca atgcaaaaag cttgtcccaa atagaaaaaa acagagccga agaaatgttt
      181 gatgatttaa agtttgacaa cgaggacaat gtggcgggtt ggccggaaga actaaaagaa
      241 cgctatggag aagttgaaaa gataatcggg gatttggaag agaatgatcg gatcaaccaa
      301 aaagaagcaa tgggagaagc cgcggcttga ttttgaagca atactgcaaa caaaataaat
      361 cgctgccctt ctcaaaaggg tcggaccctt tcaaaaaggg tccgaccctt ttaaattacg
      421 agagggataa aatggatgaa tggaaatttg gatttttggg gcgagggagg taaaatggaa
      481 taatggaaga atttgcaaac aatgatttct ggcgtcttct tgacgaaagt tattacgggc
      541 tggggcgcgc cgccgcgccg ctggagaaaa ccgctaccct gccggcgttt ttgccgtggg
      601 cgattctggg cggcgttctg ctggcgggat tgctggcggg cgcgtggttt attctcaaaa
      661 agcgaaaccg ggaaaaattc cgcttgtcaa tggacatgat cttgctgtcg gtacgaattt
      721 cgcccaaaaa cgccgaagag atccagcaat ccggacggca ggaaaaagat tggattcgcg
      781 tgatggaaga tttctatgcc aatttggtgt cgctgtcgcc gcgcggtttt ttggcgccca
      841 agccgtgggt ggcgctggaa gttgccaaag tgaaagggga gatatgtttt tatgccgccg
      901 cgccgcgccg ctatgtcagc tttttggaaa aacggatcaa cagcatctat ccggacgcgc
      961 aaattgaaaa aagcaacgat ttcaacattt tcggccccaa agaatcggtt ttttgcggct
     1021 ttgccaaaac catccggccg gtatttttgc cgttgaaaac ctatgaatcc atcggcaccg
     1081 atccgctttc ggcgatcacc aacaccctca ccaagctgga agaaaacgaa gaggccgcgg
     1141 tgcaaattgt tttgcaaaac gtttcggaaa tgtggcgtgt ccgcgggcgc gaaattctgg
     1201 acaaaatcaa aaaaggcaag aaattttccg cggcaatgga ggaaaccggc ctgatgggcg
     1261 cggcccgcgg cggcgccaaa gaaaacgaac cccaatcgtt tatgggaaat cagggcgacg
     1321 aagagctggt caaggccatg gaaagcaagc tcaacaagcg caatctggcg gccaatatcc
     1381 gcattgtcgt ttccatcaaa gacgaattcc gcagcgagca gatatttgac cagctggccg
     1441 ggtcgctgga tcagttttct tccccggggt tgaacgggtt ccgcgtggtg aaaaagaaag
     1501 gcaagtccgc cgccgcgatt ttaaacgatt acattttccg caatttcaat cccgccaaca
     1561 aatcaatttt gagcgccgaa gaattggccg gcatttttca ttttcccaca ccctatctga
     1621 aaactcccaa tatcaaaacg atcaacgcca aaaccgcgcc gcc
//
LOCUS       DAOQWE010000173         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_89902_length_1657_cov_2.246567, whole genome shotgun sequence.
ACCESSION   DAOQWE010000173 DAOQWE010000000
VERSION     DAOQWE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89902_length_1657_cov_2.246567"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PKK37_04450"
     CDS             complement(<1..878)
                     /locus_tag="PKK37_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNR81661.1"
                     /translation="MTIINNDINDGETIKTRLSNGISLARIFAMLWIFSINLLPFAFG
                     MYYDKPYEYALENNGNFIELANSNTWWADRAYFFFAFSGWTGVILFVFLSGFSMWLSI
                     LSSRKFDLKSFFLKRFNSVYVPYFFAAMAAFFVVVVFLRRMPGDYDFVSLLMGASKLV
                     PRVQAYNSPLWFITLIFSCYLLFPIIPALYSKFRFKGIMLVFATSSAVWLYAWGGSHY
                     LAQAFKVSMYPIVPFFVIMCLGVIAAHSIHNYFGSDFRKKFKKYENNFIKTAIVFYSI
                     FLYVFFGEISFDPEDQ"
     gene            complement(882..>1657)
                     /locus_tag="PKK37_04455"
     CDS             complement(882..>1657)
                     /locus_tag="PKK37_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR81662.1"
                     /translation="AKGDFIIFMGAHAEYALDYVSRCVTAAEKSNADNVGGIAKISSC
                     QGLPAKAIAGVLSSPFGAGDARYKTLLPKEPIETDTVFGGCYKKEVFSKIGLFDERMI
                     RSQDMEFNMRLKKAGGKIMLFPDIVVNYYPKSNFREFFVHNFWDGVWAIWPLRIMKTT
                     FKLRHYLPLFFVLSFLGLGILSFIWQPFLFLLFFEAGLYFVASIYFSAKISFNEKEWR
                     LLFFLPIAFAARHFGYGVGSVFGLIKLLSKDNGAFSKKS"
BASE COUNT          612 a          372 c          269 g          404 t
ORIGIN      
        1 cattggtctt ctggatcaaa agatatttcg ccaaaaaata catacaaaaa aatggaataa
       61 aaaacaatag cggtttttat gaaattattt tcatattttt taaatttttt cctaaaatcc
      121 gaaccaaaat aattatggat tgaatgggcc gcaataaccc caagacacat tatcacaaaa
      181 aagggaacaa taggatacat gctcaccttg aatgcttgag ccaaataatg agaccccccc
      241 caagcataaa gccaaaccgc cgaagaagtc gcaaacacga gcataatccc cttaaaacga
      301 aacttgctgt atagagcagg aataatggga aagagcaaat agcaagaaaa aattaaagtg
      361 atgaaccaaa gcggcgaatt atacgcttga acgcgcggca ccaattttga tgcccccatt
      421 aataatgaaa caaaatcgta atctcccggc atccgacgca aaaaaacaac tacaacaaaa
      481 aatgccgcca ttgcggcaaa aaaatatgga acataaacgc tgttaaatct ttttaagaaa
      541 aaacttttca aatcaaattt acgcgaactc aatattgata gccacattga aaatccgctc
      601 aaaaaaacga aaagaataac ccctgtccag ccggaaaaag cgaaaaagaa atatgcccta
      661 tccgcccacc aagtgttgga atttgccaat tcaatgaaat taccgttatt ttcaagcgcg
      721 tattcatatg gcttatcata atacattccg aaagcaaacg gcaaaagatt aatggaaaat
      781 atccaaagca tcgcaaaaat tcttgccaaa gaaatgccat tggacaatct cgtttttatg
      841 gtttcgccgt cattaatgtc gttatttatt atcgtcatat tctaagattt tttactgaaa
      901 gcaccgttat cctttgaaag caatttgatt aatccaaaaa cagaaccaac tccatagcca
      961 aaatgccttg ccgcaaaagc tatcggcaag aaaaataata agcgccattc tttttcatta
     1021 aacgaaattt tagcggaaaa ataaatgctt gcgacaaaat ataggcccgc ctcaaaaaac
     1081 agtaagaata aaaacggctg ccagataaaa gataaaatcc ccaaccccaa aaaacttaaa
     1141 acaaagaaca agggcagata atgacgcaat ttaaaggttg ttttcattat tctcaacggc
     1201 catatcgccc aaactccatc ccaaaaatta tgaacgaaaa actcacgaaa atttgatttg
     1261 ggataataat taacaacaat gtcgggaaaa agcataattt tgccccccgc ttttttcaag
     1321 cgcatattaa actccatatc ctggctacga atcatccgct cgtcaaacag accaatcttg
     1381 ctaaatacct cttttttgta gcacccgcca aataccgtgt ctgtttcaat cggctctttg
     1441 ggcaaaagcg ttttatagcg cgcgtcaccc gcgccaaaag gcgatgataa cacgccggca
     1501 attgcttttg ccggcaatcc ttggcaagat gatattttgg caattccgcc cacattgtcc
     1561 gcattggatt tttccgccgc ggtcacacac cgcgaaacat agtccagcgc gtattcagcg
     1621 tgcgcgccca tgaaaatgat aaaatcgcct tttgcat
//
LOCUS       DAOQWE010000174         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_90684_length_1648_cov_2.619586, whole genome shotgun sequence.
ACCESSION   DAOQWE010000174 DAOQWE010000000
VERSION     DAOQWE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90684_length_1648_cov_2.619586"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKK37_04460"
     CDS             <1..193
                     /locus_tag="PKK37_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81663.1"
                     /translation="VLYYSGIVLQWIEENHPEFILLLEEMSGRRQIELLGGGYYSPLF
                     PILQLSDKVGTGRWAEVNV"
     gene            267..>1648
                     /locus_tag="PKK37_04465"
     CDS             267..>1648
                     /locus_tag="PKK37_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81664.1"
                     /translation="MNGLIGALLIGAGLFLSRVTWIDIVQTPESDAFRLIQFHFILTG
                     ALSLWSSSTDKRGSILASLSLLNLGANGWLAFLAIVARDPTLFWPMLFVAASGINFLY
                     LLAADLNDETKASDIADDDGDDDLVPSQLGAVSAGSTLGGIAPRVGAIVLYIAALFAF
                     LFFGTIYAQLLKATIIDRSDISFEAVIANIRNGAANLALVQFAATAIVVAVLYGAIFL
                     VEALINISRRNSQKSALADFNRDLDQRERFFIRDCLASLLAYLDHRSFDPKWGRLYAI
                     GVVGVIASFIAVPWAAIMLEEIFGVLLEAQRIVGSDPVFYSGPLYIGAAIGGFFAGAG
                     LFWSLYQYLGARFPAFGEYLFARAGWNSLEGRPRTPSELLQVIVRQVRSASLNPNETF
                     DPFAFLTSAFRESEGLVYKATAWSVVATVILVAADIARFTVVDDAGVHYSKYLQLASH
                     RVPFEEIDRI"
BASE COUNT          298 a          450 c          459 g          441 t
ORIGIN      
        1 ggtcctctac tactcgggga tagttcttca gtggatcgag gaaaatcacc ctgaattcat
       61 cctgcttttg gaggaaatgt ccggacgaag gcagatagaa ctcctggggg ggggctacta
      121 cagcccgctc ttcccgattc tgcagctttc ggacaaggtg ggaacggggc ggtgggcgga
      181 agttaatgtc taggattgcg ctgaattgtc gtaaagctac acttgcggca acaaacgggg
      241 gatgcttgga tacgctcatt agatatgtga acggcctgat cggcgcactt ctgatcggcg
      301 ccgggctgtt cctgtcgcgc gtcacctgga tcgatatcgt tcagacgccg gagtcggacg
      361 cattccggct cattcagttt catttcattc tgaccggcgc gctctcgctt tggtcgtcgt
      421 caacggacaa gcgggggtca attctggcga gccttagctt gctcaatctt ggcgccaatg
      481 gttggctcgc atttttggcg attgttgcgc gcgatccgac cttgttttgg ccaatgctct
      541 tcgtcgcggc atcggggata aactttcttt acctgctcgc ggcggacctt aacgatgaga
      601 ctaaagcttc ggacattgcg gatgacgacg gcgatgacga tcttgtcccg tcgcaattag
      661 gcgccgtcag cgcaggttcc acactcggcg gcatcgcgcc gcgggtcggc gcgattgttc
      721 tttatatcgc cgcattattc gccttcctgt tttttggaac gatctacgcc cagcttctga
      781 aagctacgat catcgatcgc agcgacatat cctttgaagc tgtcatcgcg aacatccgca
      841 atggcgcggc aaatctcgct ctggtccaat tcgccgccac ggccatcgtc gtcgccgttt
      901 tgtacggcgc gatttttctg gtagaagcgc tgatcaatat ttcccgaaga aacagccaaa
      961 aatccgctct tgccgatttc aatcgcgatc tcgatcaacg cgaacgcttc tttatccgtg
     1021 attgccttgc ttcattgctc gcctatctcg atcaccgcag ttttgaccct aagtggggcc
     1081 gtctttatgc gatcggcgtt gtcggcgtca ttgcgtcttt tatcgccgtc ccgtgggcgg
     1141 ctataatgtt ggaagagata tttggtgtgt tgctagaagc tcaacggatc gttggaagcg
     1201 atcctgtatt ctattccggc ccactatata tcggggctgc gatcggcggc ttcttcgcgg
     1261 gcgcgggttt gttctggtcg ctctatcagt atctcggcgc ccgcttcccc gcgtttggcg
     1321 aatatctgtt cgcgcgcgcc ggctggaaca gtctggaggg cagaccgcgc acgccgagcg
     1381 aattgcttca ggttattgtg cggcaggtcc gctccgccag ccttaatcct aatgagacgt
     1441 ttgatccttt cgcgtttctg acatccgcgt tccgtgaatc tgaaggactt gtttacaagg
     1501 ccacagcgtg gagtgtcgtc gcgacggtca ttcttgtcgc cgccgatatt gcgcggttta
     1561 cggttgtgga cgacgccggc gttcactatt cgaaatacct tcaattagcc tcgcaccgcg
     1621 tcccctttga agagattgat cgcattga
//
LOCUS       DAOQWE010000175         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_93021_length_1623_cov_2.371811, whole genome shotgun sequence.
ACCESSION   DAOQWE010000175 DAOQWE010000000
VERSION     DAOQWE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93021_length_1623_cov_2.371811"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..316)
                     /locus_tag="PKK37_04470"
     CDS             complement(119..316)
                     /locus_tag="PKK37_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81665.1"
                     /translation="MENQEMNWDDLTDGQRELAGYLAGINEDQLAALRDLLTATIKRN
                     AVERDAFNFNSDGLRFNVNMN"
     gene            complement(333..1286)
                     /locus_tag="PKK37_04475"
     CDS             complement(333..1286)
                     /locus_tag="PKK37_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR81666.1"
                     /translation="MNELVSIDGPAGAVTTPAAVADFGGLVDLFLRSQDIRDASKRTY
                     RRALKQFGRWIEAAPRGQFTRETVLQFKTDLIGRGLSAFTVSCYLTAVKRFFTWAEGA
                     GYFPNIAKDIKLMKRGRAFQKDAFTPDQARELLAAIKTRTLRGKRDFAIVNLMIRTGL
                     RTVEIIRADAGDIANQGGKTVLYIQGKGRDGKSDFVVLTPATLTPINSYLKTRGAVDP
                     GAPLFAAVSNRNHGGRLTTRSVSRLTKSAIERIAPGRDRLTAHSCRHSAVTFALLGGA
                     TVQEARALARHADINTTLIYAHNIDRLAAAPEIKIDTVLNG"
BASE COUNT          335 a          464 c          423 g          401 t
ORIGIN      
        1 gaggataacc agggaggaaa cggcgttcaa tgatccggaa agcagcgttt cgcccttttt
       61 ggtttttgat ttccaaagtc cggttaactt gactaaatcg ctcattttgt ggccctcttt
      121 aattcatgtt tacgttgaat cgcaatccat cgctattaaa gttgaaagcg tcccgttcaa
      181 ccgcgttccg tttgattgtc gcggtcaaca gatcccgaag cgccgccagc tggtcctcat
      241 taattcccgc cagatatccg gcaagttcgc gctgtccgtc cgtcagatca tcccagttca
      301 tttcttgatt ttccattctc ccgcctccct ggttaaccgt tcagaaccgt atcgatcttg
      361 atttccggcg ccgccgccag ccggtctata ttgtgcgcgt aaatcagggt tgtattaata
      421 tcggcgtggc gcgccagcgc ccgcgcctct tggaccgtgg cgccgcccaa aagcgcaaac
      481 gtgacggccg aatggcggca gctgtgcgcc gtcagccggt cccggccggg agcgattctt
      541 tcaatagcgg atttcgtcag ccgcgaaacg ctccgggttg tcagccggcc gccgtggttg
      601 cggtttgaaa cagccgcgaa caatggcgcg ccgggatcaa ccgcgccgcg ggttttcaga
      661 tagctattta tcggcgtcag ggttgccggc gtcaatacca caaaatcgct tttcccgtcc
      721 cggcccttgc cctggatata aagaacggtt ttccctccct ggttcgcaat atcgccggcg
      781 tccgcgcgga taatctccac ggtccgaagt ccggtccgga tcatcaagtt tacaatggca
      841 aaatcccgct ttccgcggag ggttcgcgtt ttgatagcgg ccaacagttc gcgcgcctgg
      901 tccggcgtga aagcgtcttt ctggaacgcc cggccgcgct tcattaactt gatatctttt
      961 gcaatattcg ggaaatatcc ggcgccctcc gcccacgtga aaaagcgctt gaccgcggtc
     1021 agatagcagg aaacagtaaa cgccgacaac ccgcggccga tcagatccgt tttaaactgg
     1081 aggacggttt cccgcgtgaa ttgaccgcgc ggcgccgctt cgatccagcg gccaaactgt
     1141 ttcagcgccc gccgatacgt ccgtttgctg gcgtcccgga tatcctggga acgtagaaac
     1201 agatcgacca aaccgccaaa gtccgcaacg gccgccggcg tggtgaccgc tcccgccggt
     1261 ccgtctatgg aaacaagttc gttcatggtt cgcgcctccg cttgattggg gttccgtccc
     1321 tggtcccaat atagcgatta ttcgttttat tgtcaagtga atttatattc tttaatattg
     1381 tattgaaagc gttccgttct attatatttc gtttctgcca tattgtcata cgccccttaa
     1441 ggcgctaact tattgataaa taacaaccgg tgtgtgcata atacagatta tgttaacata
     1501 gaaccgcggc gacgggtttg ccggtccgct ggcgccggcg tctatatctt gcggcggccg
     1561 tcagctggaa aacgtcaata tatagaaata aaaaagcccc cagggggtgg gggcgaatga
     1621 atc
//
LOCUS       DAOQWE010000176         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_93567_length_1617_cov_4.850832, whole genome shotgun sequence.
ACCESSION   DAOQWE010000176 DAOQWE010000000
VERSION     DAOQWE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93567_length_1617_cov_4.850832"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKK37_04480"
     CDS             <1..314
                     /locus_tag="PKK37_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81667.1"
                     /translation="AVCPRRFGQIGKKTWIKSMEIEYKPTFIKQYACFENSLKEEIKE
                     KIELFRDEKNHKRLKVHKLKGKLSKYYNFSVNYKYRIVFIYQSRSRAALLAIGDHAVY
                     K"
     gene            413..1450
                     /locus_tag="PKK37_04485"
     CDS             413..1450
                     /locus_tag="PKK37_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR81668.1"
                     /translation="MQQLNQRQQKVMDFISKNKAAGNSQIAAFLGNEVTRFTVLRDLE
                     LLLGAGLIKKEGKGRAVRYLISASGDINAFFDPELYFAADQDRRNIKPVFEPKIIDEL
                     PLLFSEREINELKKINSKYLEKIKKADALFIKKEVERLTIDLSWKSSRLEGNTYSLFD
                     TEILIKERIEARGHQKEEAIMILNHKNAIDHIFEKKNEFKKIDLFQVEKIHELLTRGL
                     GVRSGIRNIPVRIIGTKYVPLSGKAKIAKMMDLATIKINSLADPFSKALAAALMISYI
                     QPFEDGNKRTARIMANAVLLADNVCPISYRSVNESDYKKAVVLFYEQNSARFFKDLFV
                     EQFKFAVDSYF"
BASE COUNT          545 a          268 c          366 g          438 t
ORIGIN      
        1 gagccgtctg tccgcggcga tttggacaaa ttggcaaaaa aacttggata aaatctatgg
       61 agattgaata caagccgact tttataaaac aatatgcttg ttttgaaaat tcattgaaag
      121 aggaaataaa agaaaaaata gagttgttta gagatgaaaa gaatcataaa cgattgaagg
      181 tccataaatt gaaaggcaaa ttatcaaaat attacaattt ttcggtgaat tacaaatacc
      241 ggattgtttt tatctatcaa tcaagatcgc gcgcggcgct tcttgcaatc ggcgatcacg
      301 ctgtatacaa ataaccaatt aagcaggggc ggagagcgtt tgccggacgg accttacgaa
      361 ggtcaataat tgttgcataa tcgtatcaaa atgctacaat tagggtaaaa ttatgcaaca
      421 attaaatcaa agacagcaaa aggtaatgga ttttatttcc aagaataaag ccgctggcaa
      481 ttcccagatt gcggcttttt tgggaaacga ggtgacgcgc tttacggttt tgcgcgattt
      541 ggaattgttg cttggcgcag gattgatcaa aaaggaaggc aaaggaaggg cggttcgtta
      601 tttgataagc gccagtggcg atattaacgc cttttttgat ccggagttat attttgccgc
      661 cgatcaagat cgaagaaata taaaaccggt tttcgaacca aaaatcattg atgaactgcc
      721 gcttttgttt tcggaacggg aaattaacga attgaaaaaa ataaactcta aatatcttga
      781 aaaaataaaa aaagcggatg cattgtttat caaaaaagaa gtggagagat tgaccattga
      841 tttaagctgg aaatcgtcgc ggcttgaagg caacacttat tcgctgtttg acactgaaat
      901 tttgatcaaa gaaaggattg aggccagggg ccatcaaaag gaggaggcga ttatgatttt
      961 gaatcacaaa aacgcgattg atcatatttt tgagaaaaaa aacgaattca aaaaaataga
     1021 tcttttccaa gttgaaaaaa tccacgaatt gctaacaagg gggttgggtg ttcgttccgg
     1081 cataagaaat attcccgtga gaattatcgg aacaaaatat gtgccgcttt ccggcaaggc
     1141 aaaaattgcc aaaatgatgg atttggcgac tattaagata aattcgctgg ccgatccttt
     1201 ttcaaaggca ttggccgcgg cgttgatgat ctcatatatc cagccgtttg aagacggcaa
     1261 caagcggacg gccagaatta tggccaatgc cgtgcttttg gccgataacg tctgtccgat
     1321 ttcttatcgg agcgttaatg aatccgatta taaaaaagcc gtggttttat tttacgaaca
     1381 gaacagcgcc cggtttttca aggatttatt tgtggaacaa ttcaaatttg ccgtggatag
     1441 ttatttttag cggttgattt gcggaaggca aaacaagggt gaagattgat cgtcagacgg
     1501 ctctgtcaat cactgtcaaa aaaattgcgc cagaagtttg aattcagcat gcaaaaacgg
     1561 gccttgataa tatttcctcc ggcgtttaaa atgaattaaa aggcaaataa aacgctg
//
LOCUS       DAOQWE010000177         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_94967_length_1603_cov_3.452196, whole genome shotgun sequence.
ACCESSION   DAOQWE010000177 DAOQWE010000000
VERSION     DAOQWE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94967_length_1603_cov_3.452196"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..212
                     /locus_tag="PKK37_04490"
     tRNA            140..212
                     /locus_tag="PKK37_04490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:173..175,aa:Arg,seq:gcg)
     gene            379..1335
                     /locus_tag="PKK37_04495"
     CDS             379..1335
                     /locus_tag="PKK37_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81669.1"
                     /translation="MENFESKSIEKHSLKENLARAEMLPPVKNSSILKEQAAYCLAWK
                     DIINPESKQGETIMYNAMGPDFLTAFLVSNAEIIYGIDSNLPKYEKLIECFTDWDSID
                     VVTSSVKPKEHSHDFIDKQSHQKSLQKTIESRAKQGFWDVPGISAQSIESCIIIEMKK
                     MGIDKTSVKIEKTNGQIKINFDWAYPEEETRRRSVAYIARNINDIINDPNRCGITSID
                     GYYEKSMNEPRFSFLSTASNDLPFISQLINPNGFALIGRSQFGLFNMENEERRLEQVT
                     RDQFGPTYDAVTIDPNYRTMMEGVFGKKDYNWSLFGMVKRDQ"
BASE COUNT          545 a          296 c          319 g          443 t
ORIGIN      
        1 ctatccaaaa acgccaaaat tggcaaatcc ccgattttat gttatcgtga acccgtcata
       61 aattgttgca gttttggaat ttttctataa actattaact gacaactata aactatcccc
      121 cgccaaaggc gggggatccg tccccgtagt ttaatggata aaatacaagg ttgcggacct
      181 tgagactaca ggttcgattc ctgtcgggga cacaaaaaaa tctacggcaa acaccgtaga
      241 tttttcgttt atagtttata gctaatagtt tttagaaaac tgcattgaac ccccaatgca
      301 gtttttgtaa atttttcaat tttaggtaat atcaaataaa atctcggaaa aaatttgaaa
      361 aacctaatta gaattgatat ggaaaatttt gaatcaaagt caatcgaaaa acatagctta
      421 aaggagaatc tcgcccgggc cgagatgctt cctcccgtga aaaatagttc tatactaaaa
      481 gagcaagcag cttattgttt ggcttggaaa gatataatca acccagaatc aaaacaaggc
      541 gaaactatta tgtacaatgc gatgggaccg gattttttga cagctttttt ggtgtcgaac
      601 gcggaaataa tttatggcat tgattctaat ttgccgaaat acgaaaagct gattgaatgt
      661 tttacagatt gggatagcat tgatgtcgtt acaagctcag tcaaaccaaa agaacacagc
      721 cacgatttta ttgataaaca aagccatcaa aaatcattac aaaaaacaat cgaatcgcgc
      781 gccaaacaag gattttggga cgtgccagga ataagcgcgc agtcaattga gtcttgcatc
      841 attatcgaaa tgaaaaaaat gggaatagat aaaacttcag tgaagatcga aaagaccaat
      901 ggacagatta aaattaattt tgattgggcg tatccagagg aagaaacgcg gcgccgatca
      961 gttgcataca tcgcccgaaa catcaatgat atcatcaacg atccaaatcg ttgtggcatc
     1021 accagtattg acggctatta cgagaaatca atgaacgaac ctcgttttag ttttttgagt
     1081 accgcaagca acgatttgcc attcatatca caattaatta atccgaacgg ctttgcctta
     1141 atcggacgat cgcaattcgg ccttttcaat atggaaaacg aagaaagaag attggaacaa
     1201 gtgactcggg atcaatttgg gccaacttac gatgccgtta ccattgaccc aaattacagg
     1261 actatgatgg aaggtgtttt cggaaaaaaa gattacaatt ggtctctgtt cggaatggtc
     1321 aaaagggacc agtgattttg catccgctta ttaccgtgtc ttacgaaatt agcttttact
     1381 gatgttccat aattagcttg ctatgttttt agcctcaaaa tgatgcaagt taattgtgaa
     1441 tcgtcagtag tgtatgtata cggtaatccc accagttttt atatataaag atttgtatgt
     1501 aatcaattgc gtaattgatt gcgtaatccg cggcgcggga tgcaaggata tatgcagtaa
     1561 tacacataaa aaaatagcaa aaaggcgcgg atgccgcgcc cca
//
LOCUS       DAOQWE010000178         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_95419_length_1599_cov_1.906088, whole genome shotgun sequence.
ACCESSION   DAOQWE010000178 DAOQWE010000000
VERSION     DAOQWE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95419_length_1599_cov_1.906088"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..995
                     /locus_tag="PKK37_04500"
     CDS             60..995
                     /locus_tag="PKK37_04500"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNR81670.1"
                     /translation="MKISFDIIIEKLNGIKYEIKGEPIDGLTGFVALNEAGKKSVSFC
                     KYRGDASRTALAGTKASLVFCWPDAILPDDNQRTYILIDNPRMHFFRILASFLSDRVD
                     YSVADSAKIGKNCELGKISIGANVVIGNNVVIGDGTIITANVVIGDGTSIGRDVLIKS
                     NTVIGQKGFGFEFDENKTPFAIPHIGGVRIGDNVEIGSNCTVVRGVLGDTIVSDFTKV
                     DDHVHIGHNVEIGAKTMITACAEISGSVKIGNNCWLGPNCSLMNQIRIGNNVLIGLGA
                     VVTKSLPDNAVAVGNPARIVRIKEEKESPWKGISR"
     gene            974..>1599
                     /locus_tag="PKK37_04505"
     CDS             974..>1599
                     /locus_tag="PKK37_04505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR81671.1"
                     /translation="MERNLPLVSVVVPCRNEEKFIGQCLESIIRQDYPKEKLEVLVVD
                     GMSNDKTRGVVARYVGEHPFIKLLDNFEIFTPFALNIGIKKSQGEIIIRMDAHAKYSQ
                     DYIVECVKNLQKYNADNVGGSLVVVASKNGSLFSRAIAWALSSVFCAGNAHYKTIHSG
                     SPRFVDTVFGGCYKRELFDRMGLFNEKLPRCQDIDFNMRLTSIGGKIL"
BASE COUNT          461 a          260 c          401 g          477 t
ORIGIN      
        1 gattttgccg aagcctttga gggggtaagg ttcttttgtt aggttattga taccaaaata
       61 tgaagatatc gtttgacata attattgaaa aacttaatgg gattaaatat gaaatcaagg
      121 gtgagccgat tgatggattg actggttttg tcgccctaaa tgaagcggga aagaaatcag
      181 tatctttctg taaatatcga ggagacgcat cccgaacggc attggccggg acaaaagcct
      241 ctttggtttt ttgttggccc gatgcaattt tgcctgacga taatcaaaga acctatattc
      301 ttattgataa tccgcggatg catttcttca gaatattagc cagtttctta tccgatagag
      361 ttgattattc ggttgccgat agtgccaaaa tagggaaaaa ttgtgaattg ggcaaaattt
      421 caatcggggc gaatgttgtg attggcaata atgtggttat cggtgatggg acaataataa
      481 cggccaatgt ggttatcggc gatggaacat cgatcggccg cgatgtttta attaagtcaa
      541 atacggttat cggacaaaaa ggttttgggt ttgagtttga cgaaaacaaa actccgtttg
      601 caattcccca tatcgggggt gtccgaattg gtgataatgt cgaaattggt tccaattgca
      661 cggtcgtaag gggagtattg ggcgatacga tcgtttccga ttttactaag gttgatgatc
      721 atgtgcatat cggccataat gtagaaatcg gcgcgaagac gatgataact gcgtgcgctg
      781 aaataagcgg aagcgtaaaa attggcaaca attgttggct cgggccgaat tgttcgctaa
      841 tgaatcaaat ccgaatcggg aacaatgttt tgatcggttt gggggcggta gtcactaaat
      901 ctttacccga taatgccgtg gcggtgggca atcccgcccg gatagtcaga attaaagaag
      961 aaaaagaatc accatggaaa ggaatctccc gttagtttcc gttgtcgttc cttgccggaa
     1021 cgaagaaaaa tttattggtc aatgtttgga atcaattatc aggcaagatt atccaaagga
     1081 aaaattggaa gttttggtgg tggatggaat gagtaatgat aaaactagag gggttgttgc
     1141 tcgatatgtc ggtgagcatc cctttatcaa acttctggat aattttgaga tatttacgcc
     1201 gtttgccttg aatatcggaa tcaaaaaatc tcaaggcgaa ataattatca ggatggatgc
     1261 acatgcaaaa tattcccagg attatatcgt tgaatgtgtg aaaaatttgc agaaatacaa
     1321 tgctgataat gtcgggggat cgctggttgt agttgcatca aaaaatggaa gtttgttttc
     1381 tcgggcgatt gcttgggcgc tgtcttctgt tttttgcgcg ggcaatgctc attataaaac
     1441 cattcattcc ggatcacccc gttttgttga tactgttttt ggcggttgct acaagagaga
     1501 gttgtttgac agaatgggac tttttaacga aaaactgccc cgttgccaag atattgattt
     1561 taacatgcga ttgacatcaa tcggcggcaa gatactttt
//
LOCUS       DAOQWE010000179         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_96963_length_1583_cov_2.750654, whole genome shotgun sequence.
ACCESSION   DAOQWE010000179 DAOQWE010000000
VERSION     DAOQWE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96963_length_1583_cov_2.750654"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKK37_04510"
     CDS             complement(<1..343)
                     /locus_tag="PKK37_04510"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNR81672.1"
                     /translation="MGYSFCKRIFDLCGSFLGMVFSAPFFVIVSLAILIDSGGPVFFK
                     QGRVGKNGKIFKIYKFRTMVKKAPALGQNFTTPANDARITKIGHFLRRHNIDELPQLI
                     NVLKGEMSLVGP"
     gene            complement(345..1505)
                     /locus_tag="PKK37_04515"
     CDS             complement(345..1505)
                     /locus_tag="PKK37_04515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR81673.1"
                     /translation="MRKDFLPFHKASIGEKEIGEAVDSLMSGWITTGPKTKKFEEEFT
                     RRVGAEYGVAVNSCTAAMHLALAAIGIKEGDEVVVPVMTFTATAEVIAYFKATPIFVD
                     CDPDTLLMDVGSLEKKITPKTKAIMPVHYGGQSCDVDAILEIARRHNLKVIWDAAHAF
                     PTFYRGKNIGSFPDIVCFSFYATKTLATGEGGMAVTNNKEYADKMRVLSLHGMNRGAW
                     NRYSKEGSWHYEIVAPGYKYNLTDIASSLGLVQLSRADELLEKRCRVAQCYSGAFGGR
                     KEISPLAIMPYGQTAWHLFVVKLNLGKLIIDRARFIDELKKRNIGSSVHFIPLHLHPY
                     WKETYGLKENDFPVASAVYRQIVSLPIFPDMSDKDVDDVITAVKEIIRLYGK"
BASE COUNT          454 a          408 c          304 g          417 t
ORIGIN      
        1 gcggtccgac caaactcatt tctcctttca agacattaat caactgcggc aattcatcaa
       61 tgttgtggcg acgcaaaaaa tggccgattt tcgtaatcct tgcgtcattt gccggcgttg
      121 taaaattttg ccccagcgcc ggcgcttttt taaccatagt ccgaaatttg taaattttga
      181 aaatttttcc gtttttccca accctgcctt gtttaaagaa aaccggcccg ccggagtcaa
      241 ttaaaattgc caatgaaaca atcacaaaga acggcgccga aaaaaccatc cccaaaaacg
      301 aaccgcacaa atcgaaaatc ctcttgcaaa aagaataccc catattattt tccataaagg
      361 cgaattattt cctttaccgc ggttatcacg tcatcaacat ccttatcgga catatccgga
      421 aatatcggca acgaaacgat ctgccgataa accgcggacg ccaccggaaa atcattttct
      481 ttaaggccat acgtctcttt ccaataagga tgcaaatgaa gcgggataaa atgaacgctg
      541 gaaccgatat ttcttttttt taattcatca ataaaccggg cgcggtcaat aatgagtttt
      601 cccaaattaa gtttaaccac aaacaaatgc caggcagttt ggccataggg cattatggca
      661 agcggcgaaa tttctttccg gccgccaaac gcgcccgaat aacattgcgc aacccggcat
      721 cttttctcca aaagttcatc ggcgcgggac aattgcacca agcccagcga agaagcgata
      781 tcggtaagat tatacttata gcccggagcc acaatctcat aatgccaaga gccttccttt
      841 gaatagcgat tccaagcgcc acggttcatt ccgtggagac tcaatactct cattttatcg
      901 gcatattctt tattatttgt gaccgccatt cctccttctc cggtagccag agtttttgtc
      961 gcgtaaaaag aaaaacatac aatatcggga aaagagccga tatttttacc ccgataaaaa
     1021 gtcggaaaag cgtgggcggc atcccatata accttcaaat tatgccgccg ggcaatttct
     1081 aaaatggcat caacgtcaca agactggcct ccataatgaa cgggcattat cgctttggtt
     1141 ttaggcgtta tttttttctc caacgagcca acatccataa gaagagtgtc gggatcgcaa
     1201 tcaacaaaaa tcggggttgc cttgaaatat gcgatcactt cggcggtggc ggtaaaagtc
     1261 attaccggca cgaccacttc gtccccttct tttatcccga ttgccgccag cgccagatgc
     1321 atcgccgcgg tgcaagaatt gaccgccacc ccatattccg cgccaacgcg acgcgtaaat
     1381 tcctcctcaa attttttggt tttcggcccc gttgtaatcc agcccgacat taaagaatca
     1441 acggcttcgc cgatctcttt ctccccgatt gatgctttgt ggaatggtaa aaaatctttc
     1501 ctcattttga ttgtttcttt ttaatgacaa ctaaatagtg cgttttcaaa aaaggaatct
     1561 tggcaagcaa ctcgcacgcc agc
//
LOCUS       DAOQWE010000180         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_97238_length_1580_cov_3.976393, whole genome shotgun sequence.
ACCESSION   DAOQWE010000180 DAOQWE010000000
VERSION     DAOQWE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97238_length_1580_cov_3.976393"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKK37_04520"
     CDS             complement(<1..399)
                     /locus_tag="PKK37_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81674.1"
                     /translation="MKQIRSLLWQEICGERETVCPDPKFKTNPMKKAIVAAVVPVLLG
                     VAQTAAAQLYTCTGQNNACTLCDFFDMMVRVIGITLKFVVPSAIILAVIAAGVMMMLK
                     QEDPDVWARAKKIMLTAIIGGLIAYGGWLLV"
     gene            complement(726..1340)
                     /locus_tag="PKK37_04525"
     CDS             complement(726..1340)
                     /locus_tag="PKK37_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR81675.1"
                     /translation="MKFFFLNFFKIIGAFFPARKLNKSLRVLAMVNTAMVFIVAMFSP
                     FYAVYVQDKIGGSLAVAGFSWAVYPIVAGILIFLFSQLGMKVKEQELLLAVGYFIRGL
                     VFLSYAFMGNVAQLIFTQVLWGIGSALGVPAFDATYGKHTSSEKSISEWGQWEGMAYI
                     ATGAAALISGLMIQLVGYFWLFCLMSAASIFLGIYIWLLPRDLL"
     gene            complement(1384..>1580)
                     /locus_tag="PKK37_04530"
     CDS             complement(1384..>1580)
                     /locus_tag="PKK37_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81676.1"
                     /translation="KQNRKTASVFAAALLLIFFRRRQRALRNFLEPAAGFLTAKTIAR
                     ANFFKDFNFCGSLDISAIWR"
BASE COUNT          464 a          452 c          341 g          323 t
ORIGIN      
        1 caccaacagc cagccgccgt aggcgatcag gccgccgatg attgcggtga gcatgatttt
       61 tttggcgcgg gcccagacgt cgggatcctc ctgtttcaac atcatcatca cgcccgcggc
      121 gataaccgcc agaattatcg ccgacggcac cacgaatttg agcgtgatgc cgatcaccct
      181 caccatcatg tcaaaaaaat cgcagagggt acaggcattg ttttggccgg tgcaggtata
      241 gagttgggcc gcggcggtct gggcaacgcc cagcaaaacc ggcacgaccg cggcaacaat
      301 ggcttttttc atcggatttg ttttaaactt cggatccgga cagaccgttt cccgctcccc
      361 gcaaatttct tgccaaagca aagaccggat ttgtttcatt ataattcatt cgccccaaaa
      421 aatacaaccc ccgctaccgc cgtttcaatt aaaaagcgcc cgaaccgtgt cggacggcaa
      481 acccaaaacc ttcaacactc aagcgaatgc cggcaaataa tctttttgtc ccgtccaaaa
      541 agaaagaaat tttgtcgcgc ggccgaaaaa ttcggaaaac tctgacaaaa atcgggcgca
      601 gaacctgatt acctgatttc gccgaaacga aatcattgct tcattctatc cgttgcgaaa
      661 aaaaattcaa gacttgcgcg gataaaaatt cgtggtttaa aaaatttttt caaaaattcc
      721 cggcatcaca gcagatcgcg cggtaaaagc caaatataaa tccccaaaaa aattgatgcg
      781 gccgacatca ggcaaaacag ccaaaaatat cccaccagct ggatcatcag cccgctgatc
      841 aacgccgccg cgccggtggc gatatacgcc atcccttccc attgtcccca ctcggagatg
      901 gatttttcac tgctggtgtg cttgccgtag gtcgcgtcaa acgccggcac tcccagcgcc
      961 gatccgatgc cccacaaaac ttgcgtgaag atcagttggg cgacatttcc cataaacgca
     1021 taactcaaaa aaaccaatcc gcggataaaa tatcccaccg ccagcaaaag ctcctgctct
     1081 ttgaccttca tccccaactg gctgaaaaga aagatcaaaa tccccgccac gatgggataa
     1141 accgcccaag agaacccggc gaccgccaaa ctgccgccga tcttgtcctg cacataaacc
     1201 gcgtagaacg gcgagaacat cgccacgatg aacaccatcg cggtgttgac catcgccagc
     1261 acccgcaagc ttttgttcaa tttcctcgcc ggaaaaaacg cgccgataat cttgaaaaaa
     1321 ttcaaaaaga aaaatttcat aaaaacgcga aaatggcaat ttcgccgcga ttatactata
     1381 atgttagcgc caaatcgccg atatgtccaa actgccgcaa aaattgaaat ctttgaaaaa
     1441 attcgcgcgc gcgatcgtct ttgcggtcaa aaaaccggcg gcaggttcca aaaaattcct
     1501 caaagcccgc tggcggcggc gaaaaaagat cagcaaaagc gcggcagcga aaacgctggc
     1561 ggtttttctg ttctgcttgg
//
LOCUS       DAOQWE010000181         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_98069_length_1572_cov_4.563612, whole genome shotgun sequence.
ACCESSION   DAOQWE010000181 DAOQWE010000000
VERSION     DAOQWE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98069_length_1572_cov_4.563612"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1572)
                     /locus_tag="PKK37_04535"
     CDS             complement(<1..>1572)
                     /locus_tag="PKK37_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81677.1"
                     /translation="QLGNQTQPETVYLGNQTKPETVQLGNQDQPETVYLGNQTQPETV
                     QLGNQTQPETVELGNQTPPEVVYLGNQTQPDTILLGDQDQPDTIVLGNTTQPETVPVG
                     DQTQPETIPVSNQTQPETVELDNETPPPAVCAGNGCGAAVVAQNQNNDSVYLPPTAED
                     DGPEDVSVVPSAGDGVVPESSESLPPSLPESAPGASSDETIPSASNAVVGEGGAVEDN
                     LASVASATTYATVVDVADADTDAINTDIISAGQGNEQVSDAVSRLIVLSAADAKLTVF
                     VREAAFDLRLETVDARAAAFYSRKGITAAPLYLGAGKQISAGVWELSIDLNANPLPNG
                     NYYIFAQIDRGDINVYHSTDVYIAINVVSADNREEREALEQTVAENSESVGQNEEKIQ
                     EVVRRVADNDLSQKLNLEEKTAELANLVRIIRRLENLIEEQTAEKEAIEAQIKRLNLE
                     IANLPPDVLSVIQRDKAAAREYLKNQRDVLERKIAVAVAARVDALGKKEALIAEIIGL
                     AGSENEKNTLKENIAA"
BASE COUNT          338 a          436 c          386 g          412 t
ORIGIN      
        1 cgccgcaatg ttttctttga gcgtgttttt ttcattctca ctgccggcca aaccgataat
       61 ttcggcaatc aacgcttctt ttttccccaa cgcgtcgacc cgggcggcca ccgccacggc
      121 aatttttcgt tccaaaacat ctctttggtt tttgaggtat tcgcgggcag cggccttgtc
      181 gcgctggata acggacagca catcgggagg caaattggca atctccaagt tcaatctttt
      241 tatttgcgcc tcaatcgcct ccttttcggc agtttgttct tcaattaaat tttcaaggcg
      301 gcggatgata cgaaccagat tcgccagttc ggccgttttt tcttccagat ttaatttttg
      361 agacagatcg ttatcggcaa cgcggcggac aacttcctgg attttttctt cattttgtcc
      421 gaccgattcg ctattttccg caaccgtttg ttccaatgct tccctttctt cccggttgtc
      481 agcagaaaca acattgatcg caatatagac atcggtgctg tgataaacat ttatgtcacc
      541 gcggtcaatc tgcgcgaaaa tataataatt gccgttgggc aacggattgg cgttcaaatc
      601 aattgaaagc tcccaaacgc cggcagaaat ttgtttgccc gcgcccaaat agagcggcgc
      661 ggcggtaatc cctttgcgcg aataaaacgc cgcggcgcgg gcatcaacag tttccaggcg
      721 caaatcaaat gccgcctcgc ggacaaaaac cgtcaatttc gcgtccgcgg cggaaagcac
      781 gatcaatcgc gaaaccgcgt cggaaacttg ctcattgcct tgaccggcgg aaataatatc
      841 agtattgatg gcatcagtat cggcatcggc gacatccacc accgtcgcgt atgtcgtcgc
      901 cgatgccacc gatgcaagat tatcctccac cgcgccgcct tcaccgacaa ccgcattgct
      961 ggcggaagga atcgtctcgt cggaagatgc accgggcgcc gattccggca aagagggcgg
     1021 cagtgattcc gatgattctg ggacaacccc gtctccggcg gacggaacaa cggaaacatc
     1081 ttccggacca tcgtcttcgg cggtcggcgg caaataaacg gaatcattgt tttggttttg
     1141 ggcaacaacc gcggcaccgc atccattgcc cgcgcaaacc gccggcggcg gagtttcgtt
     1201 gtccaattcc accgtttccg gctgggtttg gttacttact ggaatggttt cgggttgagt
     1261 ttgatcgccc accggaacag tttccggttg agtggtattg cccagcacaa tcgtgtccgg
     1321 ctgatcctga tcacccagca aaatcgtatc cggctgagtc tggtttccca aataaaccac
     1381 ctccggcgga gtttgattgc ccaactccac cgtttcgggt tgagtctgat tgcctaactg
     1441 gaccgtctcg ggctgagtct ggtttcccaa ataaaccgtt tcgggctgat cttgatttcc
     1501 taactggacc gtctcgggtt tggtttgatt acccagataa actgtttccg gttgagtctg
     1561 attgcccaac tg
//
LOCUS       DAOQWE010000182         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_98678_length_1566_cov_11.003309, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000182 DAOQWE010000000
VERSION     DAOQWE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98678_length_1566_cov_11.003309"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1415
                     /locus_tag="PKK37_04540"
     CDS             138..1415
                     /locus_tag="PKK37_04540"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HNR81678.1"
                     /translation="MEKELITMTQKELDKYETIKRLIAEEISVGDAAKQLNRSTRQVK
                     RLRGKVRREGAKGIVHGLRGKVGNRRTDPEIWNKAKKTILAKYPDFGPTFAYEKLIEK
                     EGVKIGEGTVRNLMIAENIWKPKRRKQNQQYRRQRERKECFGEMEQFDGCYHIWFGGF
                     ETCLLASIDDATGRIVRAVFTDSESVINVFKFWKGYIEAPGKPVAIYLDKFSTYKINH
                     KNAKDNSEMITQFQRACKELDIALICANSPQAKGRIERLFKTLQDRLVKELRLRGIGD
                     IAVANKFLEVEFIPDFNQRFAVVAKGKNDLHRKPDGAQLANLDAIFSIQSQRKVQNDF
                     TVRFKNQWIQIAKEQIVTVCRKDSVLIEERLDGALAIRLRNKYLNFKILPEKPQKSKQ
                     IITAIPAKRIVNCPPANHPWRKQIAAEIAKIAC"
BASE COUNT          514 a          346 c          358 g          348 t
ORIGIN      
        1 ggaaagatag cataaatttg ctttgtgtcc aatagttttt ccccaagtgt catttaaaaa
       61 tagtaatgtc atagtacagc tcaaatagaa atgtcatacc tggtatgatt gatgggtgta
      121 aattaacccc catcaacatg gaaaaagaat taatcacgat gacgcaaaaa gagctggata
      181 agtatgaaac aatcaaaaga ttaattgccg aggaaatcag cgttggcgac gcggccaagc
      241 aattgaaccg ctcgacaagg caagtaaagc ggctgcgcgg caaagtgcgc cgggaaggcg
      301 ccaaaggcat cgttcacgga ttgcgaggca aggttggcaa tcgcaggaca gatccggaaa
      361 tctggaacaa agccaagaaa acaatcctgg caaaatatcc tgatttcggg cctacgttcg
      421 catatgaaaa attgattgaa aaagaaggag tgaaaatcgg agaaggcacg gtgcgcaact
      481 tgatgatcgc cgaaaacatc tggaaaccga aacggcgaaa acaaaatcaa caataccgcc
      541 gccagcggga gcgcaaggaa tgtttcggcg agatggaaca attcgacggt tgttaccata
      601 tctggtttgg cggctttgaa acttgcctgc tggcgtcaat tgacgatgcc accggacgca
      661 tcgtcagggc agtgttcacc gacagcgaaa gcgtcatcaa tgtgttcaaa ttctggaaag
      721 gctacattga agcgccaggc aagccggtag ccatttactt ggacaaattc tccacctaca
      781 agatcaatca caagaatgcc aaagataaca gcgaaatgat cacccagttt cagagagcct
      841 gcaaagaact tgatattgcc ctgatttgcg ccaacagtcc ccaagccaag ggaaggatcg
      901 agcggctgtt taaaaccctg caagaccggc tggtcaagga actgcgcctg cgcggcatcg
      961 gcgacattgc cgttgccaac aaatttttag aagtagagtt cattcccgat ttcaatcaaa
     1021 gatttgccgt cgttgccaaa ggcaagaacg atctgcaccg caaaccggat ggcgcccaat
     1081 tggcaaatct tgacgcgata ttttcaatcc aatcgcaaag gaaagtgcag aatgatttta
     1141 ccgtgagatt caaaaaccaa tggatacaaa tcgcaaaaga gcaaatcgtc accgtgtgcc
     1201 gcaaagattc cgtattgatt gaggagcggc ttgacggcgc gctggcgatc cgtttgcgaa
     1261 acaaatattt aaactttaaa atcttgccgg aaaagccgca aaagagtaaa caaataatta
     1321 ccgccatacc ggcgaaaaga atcgtcaact gcccgcccgc caatcatccg tggcgcaaac
     1381 aaattgccgc cgaaattgca aaaatcgctt gttagaaatt ttctcaaaaa ataaaaacac
     1441 taattctaca aaagtcaagt atgacatttc tatttaatct ccaggtatga catttctatt
     1501 ttcatttgac aaaatcgtgt ttgggcttga cttaatgttt tagctcattt actctgtatt
     1561 aaggaa
//
LOCUS       DAOQWE010000183         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_99219_length_1561_cov_7.809429, whole genome shotgun sequence.
ACCESSION   DAOQWE010000183 DAOQWE010000000
VERSION     DAOQWE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99219_length_1561_cov_7.809429"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKK37_04545"
     CDS             complement(<1..548)
                     /locus_tag="PKK37_04545"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81679.1"
                     /translation="MQYKIAVSGAAEIGHCCDSIVLLSKEIGREIARHHCVLISGATT
                     GVPYFSAQGCKQAGGFNVGFSPASSEIEHLRRYRLPLDVFDVMIYTGADYSGRDIIMT
                     KSADAIIIACGRMGTLHEFTTAVECGKIIGVLEGSGGTADKIRGLVKGTYRGIKRIIY
                     DSDPASLVRKVAERVDAEKKKI"
     gene            complement(762..1037)
                     /locus_tag="PKK37_04550"
     CDS             complement(762..1037)
                     /locus_tag="PKK37_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HNR81680.1"
                     /translation="MNNLKQIPKFKSQEEERKFWAKNDSADYIDWSKAKVNPKFPELK
                     SSVCSISIRLPENMLYRLKELANKQDVPYQSFIKMILAERIKKESCH"
     gene            complement(1030..1338)
                     /locus_tag="PKK37_04555"
     CDS             complement(1030..1338)
                     /locus_tag="PKK37_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNR81681.1"
                     /translation="MEDIDFSKIFGFDWDSGNIDKNWEKHGVMFWESEEIFSNEPILI
                     QSEDKHSNPAEIRYYALGMTNQERLLFISFTIRKDRIRIISARTMSRKEKNIYQKQYE
                     "
BASE COUNT          405 a          377 c          315 g          464 t
ORIGIN      
        1 tttattttct ttttctcggc gtcaactctt tccgcgactt ttcgcaccag cgacgccgga
       61 tcggaatcgt aaatgattct tttgataccg cgataggttc ccttgaccaa gccgcggatt
      121 ttgtccgccg tgccgcccga tccttccaaa acgccgatga ttttgccgca ttccaccgcc
      181 gtggtgaatt catgcagagt tcccatccgg ccgcaagcga tgatgatcgc gtccgcggat
      241 ttggtcataa tgatatctcg ccccgaataa tccgcgccgg tatagatcat cacgtcaaaa
      301 acgtccagcg gcaagcgata gcggcgaaga tgttcaattt cggagcttgc cggagaaaaa
      361 ccaacgttaa agccgccggc ctgtttgcat ccctgcgccg aaaaataagg cacgcccgtg
      421 gtcgcgccgg aaatcaaaac gcaatgatgg cgcgcgattt cccggccgat ttctttggaa
      481 agcaaaacga tgctgtcgca acaatggccg atttcggccg ctcccgatac cgcgatttta
      541 tattgcatag atcagataac ttttaatttt caattttcaa tttttgcgcc agaggcgcat
      601 ctgcctccgg cggacattga attttcaatg gaacaatctt taaacggaat tcaaaattag
      661 cttaattgat tcattttacc aaacggaaca gataaagtga agaaaatact aaaagacacc
      721 gatgcatcgg tgtttttcgg aggcaaaacc tctttgaagg cttaatggca tgattctttc
      781 tttatccttt cagccagaat cattttaata aatgattggt aaggcacatc ttgtttattg
      841 gcaagctctt tcaaccggta aagcatattt tccggcaacc tgatggaaat actgcaaacc
      901 gacgatttca attcgggaaa tttcgggtta accttggctt tactccagtc aatataatcg
      961 gcactgtcat tttttgccca gaatttgcgt tcttcctctt gggatttgaa ttttggaatt
     1021 tgttttaaat tattcatatt gtttttgata aatatttttc tcctttcggc tcattgtccg
     1081 cgccgaaatg atgcgaattc tatcttttct tatcgtaaat gagataaata acagcctctc
     1141 ttggttggtc atgcccagcg cgtagtagcg gatttcggca ggattcgaat gtttatcctc
     1201 ggattggatt aaaatcggtt cattgctgaa aatttcttcg ctttcccaaa acataacccc
     1261 gtgtttttcc caatttttat caatgttgcc gctgtcccag tcaaatccaa agattttact
     1321 aaaatcaatg tcttccatta atttttatgt atatttccat aatatacaaa ctaaaacgct
     1381 tgtcaaggcc gcggactaaa aaatcccgcc atggcgggac cttttagcga ggggaataac
     1441 caatcgcgaa atcagtcaag ctacatagtg caatctggca gagactgcat ggcccaactc
     1501 aacctccgtc gctgaccggc tttctgcctg tattgggtaa aaaccctcat cccctcgcct
     1561 t
//
LOCUS       DAOQWE010000184         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_99549_length_1558_cov_4.072522, whole genome shotgun sequence.
ACCESSION   DAOQWE010000184 DAOQWE010000000
VERSION     DAOQWE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99549_length_1558_cov_4.072522"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1358)
                     /locus_tag="PKK37_04560"
     CDS             complement(111..1358)
                     /locus_tag="PKK37_04560"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81682.1"
                     /translation="MYIRRELETKIKPFLNRKEALAITGPRQAGKTTFIGHLQKELEA
                     AGKKVKFITFEKKGDLDLFSNSIEDFKTLAQNYDCVIIDEFQYAKDAGQKLKYLYDTI
                     KTKFIVSGSSSLELTFQTGKYMVGRLLDFELMPFSFGEYLPVVDADLAALRAKKTRSK
                     SVFDFEVTEGFGEEITRRLADALEKYAVWGGYPACVLSKTDLEKQKILEGIVEKYLLQ
                     DIKDLLGLATGDELMKIARFLALQTGNLVNFSELGEASGLAHKEVLKHINILEKTFIL
                     NLIRPFFTNRRTELVKNPKSYFVDSGLRNFLISDFRELQNRNDAGAVMENHARNLLAA
                     EGFKTNYWRTKSKAEVDFVVEDKGSVCPVEVKYGSKKIIGKSFYGFLERFNPKTGVIL
                     TRDYLGEEIIGKTKVKFIPLCYF"
BASE COUNT          419 a          387 c          294 g          458 t
ORIGIN      
        1 aatcgccgga ttctatttcc agattcaccc cccgcagaga gttaacttcc gtctttccca
       61 gatgataaat tttccaaact tcttccaatt tgatcagtgt tgacatagtg ttaaaaataa
      121 cacaggggga taaatttcac tttggttttg ccgatgattt cttcgcccaa ataatcacgg
      181 gtgagaataa cgccggtttt gggattgaat ctttccaaaa agccgtaaaa actcttgccg
      241 ataatttttt ttgaaccgta tttgacttca acggggcaaa cactaccctt gtcttccacg
      301 acaaaatcaa cttcggcctt gcttttggtg cgccaataat tggttttgaa tccttcggcg
      361 gccagcagat tacgggcgtg gttctccatt actgcgccgg cgtcattgcg attctgcaac
      421 tcccggaaat cggaaattaa aaaattacgc aatcccgaat caacaaaata acttttggga
      481 tttttcacca actccgtgcg ccggttggtg aaaaacgggc ggatcaaatt taagataaat
      541 gttttttcca gaatatttat gtgctttaaa acttccttat gcgccaaccc cgaagcttcg
      601 cccagttccg aaaagttcac cagattgccg gtttgcaacg ccaaaaaccg cgcgattttc
      661 atcagttcgt cgccggtggc aagccccagc aaatctttaa tgtcctgcaa aagatatttt
      721 tccacgattc cttccaaaat tttttgcttt tccaaatcgg tttttgacaa aacgcatgcc
      781 ggataaccac cccaaaccgc atatttttcc aaagcatccg ccagacgccg cgtaatttct
      841 tcaccgaatc cctcggtgac ttcaaagtca aaaacggatt ttgatcttgt tttttttgcg
      901 cgcaaagccg ccaaatccgc gtccaccacc ggcaaatatt cgccaaaaga aaatggcatc
      961 aattcaaaat caagcaagcg tcccaccata tacttgccag tctgaaaagt gagttccagc
     1021 gaggatgagc cagaaacgat aaattttgtt tttatcgtgt catatagata tttcaatttc
     1081 tgtcccgcat ctttggcata ttggaattca tcgataatca cgcaatcata gttttgcgcc
     1141 agcgttttga aatcttcaat tgaattagaa aacaaatcca aatcgccttt tttttcaaag
     1201 gtaatgaatt tcactttttt gcccgccgct tccaactcct tttgcaaatg gccgataaaa
     1261 gtggttttgc cggcctggcg cggcccggta atcgccaatg cctctttgcg attcaagaaa
     1321 ggtttgattt ttgtttccag ctcccgccga atatacataa tagcagggtt attttattaa
     1381 tttatttaat tttagcaggg ttattttctc tgtcaatccc cgccccaccg acaaaaattt
     1441 ggaaattacg ggatgatcac caagtcgcct tcggcgatgc ccgagatgat ttcgacgatg
     1501 cctttggcgc ggatgccaat ctgaacgcca acttcccgcg tcgcgtcgtc gccgacaa
//
LOCUS       DAOQWE010000185         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_101051_length_1544_cov_2.914036, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000185 DAOQWE010000000
VERSION     DAOQWE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101051_length_1544_cov_2.914036"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PKK37_04565"
     CDS             <1..727
                     /locus_tag="PKK37_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81683.1"
                     /translation="AAAAGTVEMFEKVDWHAGYQFTPEEDGYATQLCGYFDSGAKNRV
                     SLYDGNYKELAGVEVEGRGGWNCANIAPVKVKADRRYYVITRVEDDAIYFAYKSGLLP
                     REAENTVVEYGVRQLGAAGDFGEDLKKYDYMVFGLVDAKIKFVEDNSRGPQVSSPLPD
                     DDDEKSDLILSVQTNEDAACKFDREDLEYKEMEYTFGATGQKLHQQKICNLDDGPFTW
                     YVRCKGATDTNDGSTAIQFEVDD"
     gene            898..>1544
                     /locus_tag="PKK37_04570"
     CDS             898..>1544
                     /locus_tag="PKK37_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81684.1"
                     /translation="MVLSQKTRKFLLSIILSAGIFFCGAKLTNAVFNTGGASGQAYWN
                     ANFSAPGWGNFGSGGEEGGAGVFSEGQMKLIIEALIDPLFQEGKVNFMESLVKSAAGL
                     VVYDKMVNNTNPNYAAYYQEFINYVNTDKGRKSALENIGNLTKNTSTNSQSRTGSASG
                     PDYAGAEEYLNSIRGKGLTANQVSQGLAGFGFTCANGICKEAGTDKVATGDGSLS"
BASE COUNT          432 a          296 c          444 g          372 t
ORIGIN      
        1 ggcggcggcg gccggcaccg tggagatgtt tgagaaagtt gattggcacg cgggttatca
       61 attcacgccg gaagaagacg gctatgccac tcagctttgc ggctattttg actcgggcgc
      121 caaaaatcgg gtgagtttgt atgacggcaa ctacaaagaa ctggcgggag ttgaggttga
      181 gggccggggg ggatggaatt gtgccaatat cgcgccggtc aaagtgaagg ccgaccggcg
      241 gtattatgtg atcacccgcg ttgaagacga tgcgatttac tttgcctata aatcggggct
      301 tttgccccgc gaggcggaaa acacggtggt tgagtatggc gtccgccagc tgggcgcggc
      361 gggagacttc ggcgaagatt tgaagaaata cgactatatg gtgtttggat tggtggatgc
      421 caagatcaaa ttcgtggaag acaattcgcg cgggccgcaa gtgtcatcgc ccctgcccga
      481 cgacgatgat gaaaagagcg atttgatctt gtcggtgcaa accaacgaag acgcggcctg
      541 caagtttgat cgggaagatt tggaatacaa ggaaatggaa tatactttcg gcgccacggg
      601 gcaaaaactg caccagcaga aaatctgcaa tttggacgac gggccgttca cctggtatgt
      661 gcggtgtaag ggcgcgaccg acaccaacga cggatcaacc gcgatccagt ttgaagttga
      721 cgattgacgc ggcaaaaatc tgggtctggc ccttgcttgt tgcaatggcc agacctctga
      781 gcaaaaatga ttgataaatg ataaatgata aatagcggat agtatttttg tcagttatta
      841 tttgtcagtt attatttgcc aagtattgct tttaaaaatc aataattcaa caaaaatatg
      901 gttttgagcc aaaaaacaag gaaattttta ttgtcaataa ttttatcggc cgggatattt
      961 ttttgcggag caaaattaac caatgcagtt tttaatacgg gtggcgcttc aggacaagct
     1021 tattggaatg caaatttttc tgctcccgga tggggtaatt ttggcagtgg aggagaggaa
     1081 ggcggcgccg gagttttttc tgaaggacaa atgaagttga ttatagaagc gttgatagat
     1141 cctcttttcc aagaaggaaa agttaatttt atggaatcgt tggtaaaaag cgcggcgggg
     1201 ctagtggttt acgataaaat ggtaaataat accaatccca attatgccgc ctattatcag
     1261 gaatttatta attatgtgaa cacagacaag ggcaggaaat cggctttaga aaacatagga
     1321 aatttaacga aaaacacatc aacgaatagc caatcgcgga cgggttccgc gagcggtccg
     1381 gattatgccg gtgcggaaga atatctgaat tcaataaggg gcaagggatt gacggcgaat
     1441 caggtttccc aagggcttgc ggggtttggt ttcacttgcg ccaacggaat ctgcaaggaa
     1501 gccggcaccg acaaggtggc gaccggagat ggctccctga gcag
//
LOCUS       DAOQWE010000186         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_102706_length_1529_cov_2.322931, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000186 DAOQWE010000000
VERSION     DAOQWE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102706_length_1529_cov_2.322931"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKK37_04575"
     CDS             complement(<1..833)
                     /locus_tag="PKK37_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81685.1"
                     /translation="MFKLSLDAAYFFVADRFADYIFFSETNFNLIKYCESWMMLFLIF
                     SLMPRKKIVSSAIIWLLLAVSYVPMLTLYGFLDKPRIYIWAVTLFWLLVFFLLAIMPR
                     PKLKFLFPRQSVLVCGFIFAAMSFLGMSLLFLGIKFSINFNLVEVYRVREAFAAAKIF
                     LGGYLFNWLAYVANSFFAVWAVKNKKWWLLLSVILSQSIIFFATGNKIFLFSLFLVLG
                     LYFIARRKNVISLMFGGFSLVVFLGIAVYYVMGSEWVYFLFTRRFMMVPFAAGIQQAS
                     PT"
     gene            complement(906..>1529)
                     /locus_tag="PKK37_04580"
     CDS             complement(906..>1529)
                     /locus_tag="PKK37_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81686.1"
                     /translation="KKNEIADGVNLLFAAIFHIGIFISFQAMIFADVAVKIWLGAEFL
                     PAVPIVRILFFSAMFYCFYAASVSILNASKNKPIDTTNLFSAIVFFIFLAGAALFILP
                     IFPPTVSLACVFSATIIFLGILAYRSVREIYPQKITRDLFCVAVALAVNLPLAIISLS
                     IKPFIAENLFLTILFGVILVVAYSVLFWFLNIRRVGQLSVKNNQFYR"
BASE COUNT          618 a          325 c          266 g          320 t
ORIGIN      
        1 gtgggggatg cctgttgaat gcctgcggcg aacggcacca tcataaaccg gcgcgtaaaa
       61 agaaaataca cccactcgct tcccattaca taataaacgg caattcccaa aaaaacaaca
      121 agactaaatc ctccgaacat caaagaaatc acgttctttc ttcgcgcaat aaaatataac
      181 cccaaaacta aaaaaaggga gaacaaaaaa attttattgc ccgtggcaaa aaaaatgatg
      241 ctttgagaaa gaatcaccga taaaagtagc caccattttt tgtttttaac cgcccaaacc
      301 gcaaaaaaag aattggcgac ataggcaagc caattgaaca gatacccgcc caaaaaaatt
      361 ttagcggcgg caaacgcttc cctgacgcga taaacttcaa ccagattaaa attaatggaa
      421 aattttatac caagaaacag aagcgacatt cccaaaaacg acattgcggc aaaaataaaa
      481 ccgcacacaa gcaccgattg tcttggaaat aaaaatttta attttggccg gggcataatg
      541 gcaagcaaga aaaaaaccaa aagccaaaaa agagtgaccg cccaaatata aatccgcggc
      601 ttatccaaga agccgtaaag cgtcaacatt ggaacataag aaacggcaag caaaagccaa
      661 attatcgcgc tggaaacaat ttttttccga ggcatcaagg aaaatatcaa aaataacatc
      721 atccatgatt cgcaatattt gatcaaattg aaattcgttt cggaaaaaaa gatataatcg
      781 gcgaagcgat cggccacgaa aaaataagcg gcgtcaagcg acaacttgaa cataacccat
      841 gcgaaaaaat caaaaaaatt tttattttgg cgaagcattt tctctaaaat attaaaaact
      901 gcaatttaac gatagaattg attatttttc acggataatt gaccgacccg gcggatattc
      961 aaaaaccaaa aaagaaccga atatgccaca actaaaataa cgccaaacaa tatcgtcaaa
     1021 aataaatttt cggcaataaa gggcttaatt gataacgaaa taatcgccaa cggcaaatta
     1081 accgccaatg ctacggcgac acaaaataaa tctcgggtaa ttttttgagg atagatttct
     1141 cttaccgaac gataagccaa aattcccaaa aaaattatcg tcgcggaaaa aacacacgcc
     1201 agcgacacag tgggagggaa gatcggcaag ataaaaagcg ccgcgcccgc taaaaagatg
     1261 aaaaaaacaa ttgccgaaaa aagattggtc gtatctattg gtttgttttt ggaagcgttc
     1321 aaaatgctga ctgacgccgc gtaaaaacaa tagaacatcg ccgaaaaaaa taaaatacga
     1381 acaatcggta ccgccggcaa aaattctgcg ccaagccata tcttgaccgc cacatcggca
     1441 aaaatcattg cttgaaacga aataaatatt cctatatgaa aaatcgcggc aaacaataaa
     1501 ttgacgccat cggcaatttc attcttttt
//
LOCUS       DAOQWE010000187         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_103212_length_1524_cov_6.842069, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000187 DAOQWE010000000
VERSION     DAOQWE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103212_length_1524_cov_6.842069"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..560
                     /locus_tag="PKK37_04585"
     CDS             264..560
                     /locus_tag="PKK37_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNR81687.1"
                     /translation="MEQNQQPNQPQLQIKVRDEELKGAYSNLMQILHTKEEFVLDFFL
                     NAPPQGILTSRMIVSPGHAKRIAAALAENIKKYEEKFGKIEGSEAPEGKIGFIK"
     gene            740..1180
                     /locus_tag="PKK37_04590"
     CDS             740..1180
                     /locus_tag="PKK37_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNR81688.1"
                     /translation="MALIPYRGGWDIDRWFDDDDWMNWPSFQLMKMPEAPKMDVYEKD
                     GKVVVKAELPGFKREQIDAQIKDGVLTIEAKSEEKKEEDNKKKGYWRKEIRHGYVKRA
                     VALPEIDESGVEAEFENGVLKITAPKAAPQVEEAKAKKLEIKGK"
BASE COUNT          553 a          263 c          359 g          349 t
ORIGIN      
        1 caaggcgaga agtttaatcc cgaacttcac gaagcggcgc aaatggagga gtgcccaggc
       61 gaagaatcgg gaacgatcgt tgaagaaata caaaaaggat atatgattga cggatgcctg
      121 ttgcgcccgg caaaagtgaa agtggcaaag taaaaaaatt taaaattaaa aaatcaaaat
      181 taaaaatgac aatgtaaaat taaaaatgaa aaatacaaca atattttaca ttttgatttt
      241 tatatcttca atttttaaat attatggaac aaaatcaaca accgaatcaa ccgcagttgc
      301 agataaaggt gcgcgacgag gaattgaagg gcgcctattc caatttgatg cagattttgc
      361 acaccaagga ggagttcgtg ctggatttct ttttgaacgc gccgccgcag ggaattttga
      421 ccagccggat gatcgtgagt ccggggcatg ccaagcgaat cgcggccgcg ctggccgaaa
      481 acatcaagaa atatgaagaa aaattcggca aaattgaagg aagcgaagcg ccggaaggga
      541 aaataggatt cataaaatag ttttcaatta ccagttttca atttccaatg aatgatctat
      601 tcattgaaaa ctcaatgaaa actgaaaatt gaaaactgaa aaatttgctt ttataacaac
      661 gcttttgcca agtttgcggg caatccaaaa caggttatgt taaattaaaa attgcggacg
      721 aaacaaaagc gaaaaattta tggcattgat accttatcgc ggcggatggg atattgatcg
      781 ctggtttgac gacgatgact ggatgaattg gccgtcgttc cagctgatga agatgcccga
      841 agcgcccaaa atggatgtct atgaaaagga cggcaaagtg gtggtgaagg ccgaattgcc
      901 gggcttcaaa cgggaacaga ttgacgctca gatcaaagac ggcgtgctca ccattgaggc
      961 caaatccgaa gaaaagaaag aggaggacaa caaaaagaag ggctattggc gcaaggaaat
     1021 ccgccacggc tatgtcaaac gggcggttgc cttgcccgaa attgacgaaa gcggagtcga
     1081 ggcggaattt gaaaacggcg tgttgaaaat caccgcgcct aaagctgcgc cgcaagttga
     1141 agaagcgaaa gccaaaaagc tggaaatcaa agggaaatag aatcgtagaa tataatgcct
     1201 gttttggaaa acggcggggc gcaagccccg cctccgcctg aacggcggaa attcaaaatt
     1261 cgaagcacga aattcaaaag aaatccgaat tttcaaatgt ccaaattttt taaacaggat
     1321 ttcgttttaa aaatttgaat tttgaaaatt tgagaattgt ttcgatattc ggatttcgat
     1381 atttgaattt aaaggtaaaa taatagttgg agaaaatata aagcaaattc ttgagattga
     1441 catttaaaat aaacaattaa taaaaaaatt atggcaagaa caatcggaat tgatctggga
     1501 accacaatgt cggaaatggc cgcc
//
LOCUS       DAOQWE010000188         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_103692_length_1520_cov_2.985666, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000188 DAOQWE010000000
VERSION     DAOQWE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103692_length_1520_cov_2.985666"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PKK37_04595"
     CDS             <1..656
                     /locus_tag="PKK37_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR81689.1"
                     /translation="SNPDIRDELILKLTYHSNRIEGSTLTKDETAAILFNNATVPNKT
                     LVEHLEAKNHKTAVEYMFGHVAQKNPIDGEFIVKLHGILMNSIRDDAGIWRRHGVRIV
                     GANVPTANCIKIPELMAELVGKMDSQAKDIVGLAAKIHVEFEKIHPFSDGNGRVGRLI
                     LHAMLLRRNYAPAVILQENKRQYNLYLNKAQTGGDQSLLEDLISDAVLAGFAILERK"
     gene            734..874
                     /locus_tag="PKK37_04600"
     CDS             734..874
                     /locus_tag="PKK37_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81690.1"
                     /translation="MDKISRIKKWAMEKIDRDYRKKIFFPFAKKLVVKRYDALRELLG
                     LK"
     gene            904..1344
                     /locus_tag="PKK37_04605"
     CDS             904..1344
                     /locus_tag="PKK37_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNR81691.1"
                     /translation="MERFKIVPACYLMLVKDGKMLLSRRFNTGYEDGNYSFVAGHLNG
                     SETFRQAMAREAKEEAGIVIDPEKLKIIHVMHRAQEDSTGERMDILMTIAEWVGEIKN
                     NEPEKCDDLSWFLIDEIPDNVVPFLKQAINCVNKGIFYSEFGFV"
BASE COUNT          472 a          252 c          378 g          418 t
ORIGIN      
        1 tgagcaatcc cgatatccgc gacgaattga ttttgaaact tacctatcat tcaaatcgca
       61 ttgaaggcag tactttgacg aaagatgaaa ccgcggcaat tttatttaac aatgccacgg
      121 ttccgaacaa aactttggtt gaacatttgg aagcaaaaaa tcataaaacc gcggtggaat
      181 atatgtttgg ccatgtggcg caaaaaaatc cgattgacgg ggaatttatt gtaaaattgc
      241 acggaatttt gatgaacagc atccgtgatg acgcgggaat ttggcggcgc cacggcgtgc
      301 gcatcgtggg cgccaacgtt ccaacggcaa attgcatcaa gattcccgaa ttgatggccg
      361 aattggtggg caaaatggat tctcaagcca aagatattgt tggcttggcg gcaaaaatcc
      421 atgttgaatt tgaaaaaatc catccgtttt ccgacggcaa cggccgagtg gggcggttga
      481 ttttgcatgc aatgcttttg cgccgtaatt acgctccggc ggtgattttg caggaaaaca
      541 agcggcaata taatctttat ctaaacaaag cgcaaaccgg gggcgatcaa agtttgctgg
      601 aagatcttat cagcgacgcg gttttagccg gttttgccat tcttgaaaga aaataatcgc
      661 agttgctatg taattaatct acaggggttc aacccctgta gatttatcag gggttgaacc
      721 cctgataaac tttatggata aaatcagccg gatcaaaaaa tgggcaatgg aaaaaatcga
      781 tcgcgattac cgcaaaaaga tatttttccc cttcgccaaa aaacttgtcg ttaaacgtta
      841 tgatgctttg cgagaattgt tggggttaaa atagttttgg aaatataaga ttttaaatca
      901 attatggaaa ggtttaaaat tgtgccggcg tgctatttga tgctggtaaa agacgggaaa
      961 atgttgctgt cgcggcggtt caataccggt tacgaagatg gtaattacag ttttgtcgct
     1021 ggccatttga acggcagtga aacttttcgg caggcgatgg cccgcgaggc aaaagaggaa
     1081 gccggtatcg tcattgatcc ggagaaattg aaaataattc atgtgatgca tcgcgctcag
     1141 gaagattcaa ccggcgaaag aatggatatt ttgatgacca ttgccgaatg ggttggggaa
     1201 ataaaaaaca atgaaccgga aaaatgcgat gatttatctt ggtttttgat tgatgaaatc
     1261 cccgataatg tcgtgccttt tttgaagcag gcgattaatt gtgtcaataa aggaattttt
     1321 tacagcgaat tcggttttgt ttaaaattat gccgattaaa attttgttat catggaaata
     1381 atcttacggt ggagtttatt gagatgatgg gagctaataa tgaggtggaa tggccattgg
     1441 acaagttgcg gataacgggg tagggttaaa taaaggtcag agtttgagat aattaaaatt
     1501 aaaaataaaa atatgaaggg
//
LOCUS       DAOQWE010000189         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_105386_length_1505_cov_15.023448, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000189 DAOQWE010000000
VERSION     DAOQWE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105386_length_1505_cov_15.023448"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="PKK37_04610"
     CDS             <1..1455
                     /locus_tag="PKK37_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81692.1"
                     /translation="CPQDCGYNTPTVNLDYSGQVKCGQNATLHWTSNNATNCQAGGSW
                     SGSKATSGSEQVGNFTGSRTYTITCTGSGGSASDSVSITGTADDLEADAGPDKYINDN
                     ENSVRLDGSVDGNYDSLSWSCTGGSLSSSSSLRPTFYRPDYNYNYNDNSWDYDKTYTC
                     TLTARNECGADSDTMKVRVERDDDDQDFNVSLIARPKSDCAPLENVDLIATVSNYGSS
                     NYDRDFTYYFDCENDGHWDKTVTTDNTSYTAVNLCDYRNVGSYTARVRVERQNRTATD
                     TEIVRAEQCGSTATGDLSITKTVSNLSLGTGYQGTVSANPLNTVAYRIVVAGIGGTAN
                     NVTLTDAVPAGITNIRDLRVDGIAANGSLSSGISLGTIVSGQTKTIAYTATVANEASF
                     VYGQTTLTNVATVTADGKSANSSAAVAVNRQAVMGATTISTGFDNQTLAGLGLGLAAA
                     ILCLAWVTKTQIIDPKRDPRAILRDKIAAIRKGM"
BASE COUNT          344 a          499 c          404 g          258 t
ORIGIN      
        1 tgcccgcaag actgcggcta caacacgccc acggtgaatc ttgattattc cggccaggtc
       61 aaatgcggcc aaaatgccac cctgcactgg acttccaaca atgccaccaa ttgccaggcc
      121 ggcggttcgt ggtcggggag caaagccact tccggttccg agcaagtggg caacttcacc
      181 ggttccagaa cctacaccat cacctgcacc ggctcgggcg gcagtgcttc cgacagcgtg
      241 agcatcaccg gcaccgccga tgatttggaa gcggacgccg gtccggacaa atacatcaac
      301 gacaacgaaa attccgtgcg tctggacggt tccgtggacg gaaactacga ttcgctttcc
      361 tggtcctgca ccggcggcag tctgtcgtct tcatccagcc tgcgcccgac tttctaccgc
      421 ccggattata attacaacta caacgacaac agctgggatt acgacaaaac ctacacctgc
      481 accctcaccg cccgcaacga atgcggtgcg gattccgaca cgatgaaggt gcgcgtggag
      541 cgcgacgacg acgatcagga ttttaacgtc tcgttgatcg cgcgccccaa gtccgactgc
      601 gccccgctgg aaaacgttga tctgatcgcc acggtgtcca actatggcag ctcaaactac
      661 gaccgcgatt tcacctatta ctttgactgt gaaaacgacg gccactggga caaaaccgtg
      721 accaccgaca acacttccta caccgcggtc aatctctgcg attaccgcaa cgtcggcagt
      781 tacaccgcgc gggtgcgcgt ggagcggcaa aaccgcaccg ccaccgacac ggaaatcgtc
      841 cgcgccgaac aatgcggcag taccgccacc ggcgatttga gcatcaccaa gaccgtgagc
      901 aacctctcgc tgggcacggg ctaccagggc acggtgagcg ccaatccgtt gaacaccgtg
      961 gcttaccgca tcgtcgttgc cggcatcggc ggcaccgcca ataatgtgac gctcaccgac
     1021 gcggtacccg ccggcatcac caacatccgc gacctgcggg tggacggcat cgccgccaac
     1081 ggcagtttgt cctcgggcat cagtttgggc acgatcgttt ccgggcaaac caaaacgatc
     1141 gcctataccg ctaccgtggc caacgaagcc agcttcgtct acggccaaac cacgttaacc
     1201 aacgtggcca cggttaccgc cgacggcaag tccgccaact ccagcgccgc ggtggcggtc
     1261 aaccgccagg cggtgatggg cgcgaccacc atttccaccg ggtttgacaa tcaaaccctg
     1321 gcgggactgg gcctcggcct ggccgcggcg attctctgcc tggcctgggt gacaaagacc
     1381 caaatcattg atcccaaacg cgatccgcgc gcaatcctgc gcgacaagat cgcggccatc
     1441 cggaaaggaa tgtagaaatc cggttgattg aaatcaaaaa gctcccgcca agggagcttt
     1501 ttggg
//
LOCUS       DAOQWE010000190         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_105986_length_1500_cov_6.120415, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000190 DAOQWE010000000
VERSION     DAOQWE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105986_length_1500_cov_6.120415"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PKK37_04615"
     CDS             complement(<1..673)
                     /locus_tag="PKK37_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNR81693.1"
                     /translation="MKIAIIGAGVCGLYLAKNLAQKGNEVFVFEKKKTIGKQCCSGLF
                     SARLFDFFPEARQLETNQIGSCIINFPKRTIRIKFRPRFSVIDHAQLDLLAGALATQA
                     GANIRTGQNIDFSELDELSAGYDRVIGADGALSPTRNYLAFKKGRTLFKKGSDPFGLS
                     FPLGIQGFEQKRDNSDFVETWATKNGFLWRIPRGKDVEWGIMEKPDLAPKIFNEFIAR
                     KNIKLT"
     gene            complement(1099..1455)
                     /locus_tag="PKK37_04620"
     CDS             complement(1099..1455)
                     /locus_tag="PKK37_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81694.1"
                     /translation="MSKIAIEINQGDDCLIWKFSTLRFLIELSPWEREVMDIIAMGGK
                     CSHDYIRKKAGYAPDGELADFLEACEIHGLILRVPDCTDCFYCLTKAETNITLAQYGR
                     WFIDKGHEIKRRRARI"
BASE COUNT          395 a          329 c          305 g          471 t
ORIGIN      
        1 ccgttaattt gatatttttt cgtgcgataa attcattaaa aattttcggc gccaaatcag
       61 gtttttccat tattccccat tcaacatctt taccacgcgg gattcgccac agaaatccgt
      121 tttttgttgc ccaggtttcc acgaaatccg aattatcccg cttttgttca aatccctgta
      181 ttcccagcgg gaatgacaaa ccaaaagggt cggacccttt tttaaaaagg gtccgaccct
      241 ttttaaaagc aaggtagttt cgcgtgggcg aaagggcgcc atcggcgccg atgacgcgat
      301 catatccggc ggataactcg tccaattcgg aaaaatcaat gttttggccg gtgcggatgt
      361 tggcgcccgc ttgcgttgcc agtgcgccgg caagcaaatc caactgcgcg tggtcaatga
      421 cggaaaatcg cgggcgaaat tttatccgta tcgttctttt gggaaaattg atgatgcaag
      481 aaccgatttg attggtttca agttgtcgcg cttcgggaaa aaaatcaaaa agccgcgccg
      541 aaaacagccc cgaacagcat tgcttgccga tggttttttt cttttcaaaa acaaaaacct
      601 cattgccctt ttgcgccaaa ttttttgcca gatagagccc gcaaaccccg gccccgatga
      661 tcgcaatctt catattttat tgactttatt ttatgttttg ataccatcgc attagtagcc
      721 atctagttaa ggattaagga gcgtggtttt tgatggtaaa aggaatttgg tattggtatt
      781 acaggcattg gcctctaatg tttaaaggta tatatacgat tatatcgatc attgtccttt
      841 ggttgatatt caattcgcca acctttattg caatgttgat tatggcgggg gcaatttgca
      901 tattgatcct aatcagtcat ttgatgtctt ggttgggaga tcaataccta gttcgttacg
      961 atgatggtta ccgctggatt cgcggattgc cgccaaggag aaaaaggaga aaaacgaaac
     1021 cctaaaattc tcttgcggca tttttttatc cattaatggt attgggattt tgattttaca
     1081 ataataaaac tgcgagcatt atattcttgc tcgccgccgc ttgatttcat ggcccttatc
     1141 aatgaaccat ctgccatact gtgccaaagt tatatttgtt tcggcctttg taaggcagta
     1201 aaaacaatcc gtgcaatcgg gaacgcgcaa aatcaatccg tggatttcgc acgcttctag
     1261 aaaatcagcc agttcgccgt cgggagcata tcccgctttt tttcttatat aatcatggct
     1321 acattttcct cccatggcga tgatatccat cacctctctt tcccatgggc ttaattctat
     1381 caaaaatctc aaggtactaa atttccatat caagcaatcg tccccctgat ttatttctat
     1441 cgcaattttt gacaaaaggc aagatttgcg ctattatttg cgtgtaaatc ttataacttt
//
LOCUS       DAOQWE010000191         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_108135_length_1482_cov_8.642607, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000191 DAOQWE010000000
VERSION     DAOQWE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108135_length_1482_cov_8.642607"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..>1482
                     /locus_tag="PKK37_04625"
     CDS             71..>1482
                     /locus_tag="PKK37_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNR81695.1"
                     /translation="MKVEPQQLKTFLIDAGIITDGQFEVCATQARGKGMRVEDILVAR
                     KFIDDEQLLKLKAYILGIPFVNLEKENIRAEVLELIPEPIARANNIVAFRRTGDFLEV
                     AMLDPEDLGIIESIKKTTGLSILPRLTDPQGIKSAILRYQKTLMAEVGDLGVLPEDSG
                     GAMRVSEAPAPAEGNNSGELKKAAQDVPVIKIVDTLIKHAILERASDIHIEPTEKEVV
                     VRYRVDGVLRDAMILPKSAAAGIVARIKVLSNLKLDECRLPQDGRFKAESDQWRYSLR
                     VSTLPVFYGEKIVMRLLSESAKAYPLEKLGFCGEGLEKIKNALHKTMGMILMTGPTGS
                     GKTTTLYSMVDILNEPGVNISTVEDPIEYQMPRVNQTQVNAKIGLTFASGLRTLLRQD
                     PNIIMVGEIRDNETAALAINAALTGHLVLSTLHTNTAAGAIPRLIDMKAEPFLIASTL
                     NVIIAQRLIRCLAAGKEEYRL"
BASE COUNT          376 a          381 c          406 g          319 t
ORIGIN      
        1 tgagagtcat tgaccaaacg ctgggggctt cccgcggcga aaccgccggc caagacgatt
       61 aatccgcaaa atgaaagttg agccacaaca actgaaaact tttttgattg atgccggcat
      121 aatcacggac ggccagtttg aagtttgcgc cacgcaggcc cggggaaaag gaatgcgcgt
      181 tgaagatatt ctggtcgcgc gaaaattcat tgatgatgaa cagctgttga aactgaaagc
      241 ctatattctg ggaattccgt tcgtcaatct ggaaaaagag aatatccgcg ccgaagtttt
      301 ggagctgatt cccgaaccga ttgcccgcgc caacaacatc gtcgctttcc gccggaccgg
      361 cgattttttg gaagtggcga tgcttgatcc cgaagatttg ggaatcattg aatcaatcaa
      421 aaaaaccacc ggactttcaa tattgccgcg gctcacggat ccgcaaggaa tcaagagcgc
      481 gattttgcgc taccaaaaaa ctttaatggc cgaagtgggc gatttggggg ttttgccgga
      541 agattccggc ggcgcgatgc gggtgtcgga ggcgccggcg ccggcggaag gaaataacag
      601 cggagagtta aagaaagccg cccaagacgt gccggtgatc aaaattgtgg ataccttgat
      661 taagcacgcg atcttggagc gggcttcgga catccatatt gaacccaccg aaaaggaagt
      721 ggtggtgcgc tatcgcgtgg acggcgttct gcgcgacgca atgatcctgc ccaaatccgc
      781 cgccgcgggg atcgtggcgc gcatcaaagt gctttcaaat ctaaaactgg acgaatgccg
      841 cctgccccag gacggccgct tcaaagcgga aagcgaccaa tggagatatt cgttgcgcgt
      901 ttccactctg ccggtttttt acggagaaaa aatcgtgatg agattgcttt cggagagcgc
      961 caaggcctat cctctggaaa agcttggttt ttgcggcgag gggctggaaa agataaaaaa
     1021 cgctctgcac aaaacgatgg gaatgatctt gatgaccggt cccaccggtt cgggaaaaac
     1081 cactacgctt tattcaatgg ttgacattct aaacgaaccg ggcgttaaca tttccaccgt
     1141 tgaagatccg attgaatacc agatgccgcg agtcaatcaa acccaggtca atgccaaaat
     1201 cggcctcact tttgcttcgg gcctgcgcac tcttttgcgc caggatccca acatcattat
     1261 ggtgggggag atccgcgata atgaaacggc cgcgctggcg atcaacgccg cgctcaccgg
     1321 ccatctggtg ctttccactt tgcataccaa taccgccgcg ggcgcgattc cccgcttgat
     1381 tgatatgaag gccgagccgt tcctgatcgc gtccacactc aatgtcatta tcgcccagcg
     1441 gctcattcgc tgtttggccg ccgggaaaga agaataccgg ct
//
LOCUS       DAOQWE010000192         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_108556_length_1479_cov_2.900281, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000192 DAOQWE010000000
VERSION     DAOQWE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108556_length_1479_cov_2.900281"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="PKK37_04630"
     CDS             <1..143
                     /locus_tag="PKK37_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81696.1"
                     /translation="EKVARLADLSLNTIVKVENGVNKNPTIETLTKIAKALEVGVDDL
                     IK"
     gene            148..>1479
                     /locus_tag="PKK37_04635"
     CDS             148..>1479
                     /locus_tag="PKK37_04635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81697.1"
                     /translation="MPRKINYDNWSKEELIKELRRIKETKYGLVWHRDLPEEKIDTLI
                     NPDARTPNEMFPNEMAGKPFPILKEVKGKELNNDKDKRVNLLIEGDNYHSLAVLNFTH
                     YEAVDFIYIDPPYNTGARDWMYNNNYVDITDPFRHSKWLSLMYKRLKLAKNLLKEEGV
                     ICVTIDDNELAPLWMLMNEIFDEFSHLGTVVIRNNPKGRMTARKVSLIHEYALFFGKS
                     HLATIKKLPVAPEEKTHNYKKDKDGSWYLPVNLRKQGVDSTATNRKGKLSERYYPIYF
                     DPKTEQISTKKKLKVEILPIDQRGEKRIWRRSKDVIDEMYEKGDLWVQKTNGGYQVYF
                     KFRGGLEGQMPKSIWYDAKYSASEYGTQILDSIMGAREMFQYPKSPYAVKDCILAGTD
                     KKDAIILDFFAGSGTTGHATLELNKEDGGNRQFILCTNNENNICTDVCYPRL"
BASE COUNT          522 a          251 c          305 g          401 t
ORIGIN      
        1 ttgaaaaggt agctcggctt gcggatttat cgcttaatac catagttaaa gttgagaatg
       61 gagtaaataa aaacccgact attgaaactt taaccaagat cgccaaagcg cttgaggtcg
      121 gcgttgatga tttaattaaa taatcctatg ccaagaaaaa taaactacga caattggtca
      181 aaagaagaat tgataaaaga gcttcgccgt atcaaagaga cgaaatacgg ccttgtttgg
      241 catagggact tgccggaaga aaaaattgat acccttatca atccagacgc gcggacacca
      301 aacgaaatgt ttcccaatga aatggcggga aaaccgtttc cgattttgaa agaagttaaa
      361 ggcaaagaac taaacaacga caaagacaag cgggttaatt tactgattga aggcgataat
      421 tatcattcgc ttgcggtttt gaattttacc cactatgaag ctgttgattt tatttatatt
      481 gacccacctt acaatacagg tgcgcgcgat tggatgtata acaataatta tgttgatatt
      541 accgaccctt ttcgtcatag caaatggctt tcgttaatgt ataaacggtt aaagttagca
      601 aaaaatttac ttaaagaaga gggtgttata tgcgtaacaa ttgacgataa tgaattagcc
      661 ccgctttgga tgttaatgaa cgaaatcttt gacgagttta gtcatcttgg cacagtcgta
      721 attcgtaata atcccaaagg tagaatgact gctcgtaagg tttcactcat ccacgaatac
      781 gcacttttct ttgggaaatc acacctcgct actattaaga aattgccggt tgcccccgaa
      841 gaaaaaactc ataactataa aaaagataaa gacggttcgt ggtatttacc tgttaatttg
      901 aggaagcaag gagttgatag cactgccaca aatcgaaaag ggaaattatc cgaaagatat
      961 tatccaattt attttgatcc caaaaccgaa caaataagca caaaaaagaa attaaaagtt
     1021 gaaattttgc cgattgatca aagaggcgaa aaaagaattt ggcgacgctc aaaagatgtt
     1081 attgatgaaa tgtatgaaaa aggcgatctt tgggttcaaa aaacaaatgg cggttatcag
     1141 gtttatttca aatttagagg gggattagaa gggcaaatgc caaaaagtat ttggtatgac
     1201 gcaaaatatt cagcaagcga atatggcact caaatattgg attctataat gggcgcaagg
     1261 gaaatgtttc aatatccaaa gtcgccatat gccgttaaag attgtatttt ggcgggaact
     1321 gacaaaaaag acgctataat tttagatttt tttgctggct ctggtacaac tggccacgcc
     1381 actttggaat taaacaaaga agatggcgga aatcggcaat ttatactctg caccaacaac
     1441 gagaataata tttgcaccga tgtttgctac ccgcgtttg
//
LOCUS       DAOQWE010000193         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_109827_length_1468_cov_5.399151, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000193 DAOQWE010000000
VERSION     DAOQWE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109827_length_1468_cov_5.399151"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1368)
                     /locus_tag="PKK37_04640"
     CDS             complement(58..1368)
                     /locus_tag="PKK37_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HNR81698.1"
                     /translation="MAGNFFKNETGNSLINKARNAIFVDMQRMVQKALNPENHHLSPI
                     AKKRLNWMYELYHRQNGNISKAHRKLGISRQWLSAIKNEFEQSGKDPRSLEPDSRAPH
                     DTSKRNRISKETENLILKVRKDSRNAWGKVKIARVLQRDYGVKVHPNTANNYLRLHKL
                     INPKISLKNSNAWKAKKEREQNPQSASAKWRPPKAIKDYAPGALVERDIKFIPIPGKQ
                     LGPGLARDDFWSQHTFIDSFTRIRTLELTAGHTATDAVSCRRKIQARLPFQIASENSD
                     NGSENSGAYHQELRDTDTFHFYSNAATPTDNPRVERSHLTDDLEFYQKGGLRNTFEEQ
                     QAAIADWEYFYDWVRPHQALAYLTPMEFYQLWKRDPEAATEITQKWQNYLAKQRKRLA
                     LSRKIKRKEEIQNLMKFIDTKLDKKSSEKSIKLTLRNCQLCSMA"
BASE COUNT          304 a          324 c          384 g          456 t
ORIGIN      
        1 gtatcaacgc ggccgttttt tgtctttgcc agcggacgcc aatcggacag ggtatgttca
       61 agccatggaa cataattgac agttccgaag cgttaatttg attgattttt ccgacgattt
      121 tttgtccaat tttgtatcaa tgaatttcat taggttttga atttcttctt ttcttttgat
      181 ttttcttgaa agagcaagcc gttttctttg cttggccaga tagttttgcc atttttgggt
      241 aatttccgtt gccgcctcgg gatcgcgttt ccaaagctgg tagaattcca tcggcgtgag
      301 gtaggccaac gcttggtgcg gcctgaccca atcgtagaaa tattcccagt cggcgatcgc
      361 ggcctgttgt tcttcaaatg tgtttctcaa tccgcccttt tggtagaatt ccaaatcatc
      421 ggttaaatga gatcgctcca cgcgcggatt gtcggtcgga gttgcggcat tggagtagaa
      481 atgaaaagtg tcggtatcgc gcagttcttg atgatacgcg ccgctgtttt cactgccatt
      541 gtcggaattt tcggaagcga tttgaaacgg cagacgggct tgtattttcc ggcggcagga
      601 taccgcgtcg gtagcggtat gtccggcggt taattccaat gtgcggattc gggtaaagga
      661 gtcaataaaa gtgtgctggc tccaaaaatc atcccgggca agaccagggc caagctgttt
      721 ccccggaatc ggaatgaact taatatctct ttccaccagc gcgccgggcg cgtaatcttt
      781 gatggctttg ggaggccgcc atttggcgga tgccgactgc ggattttgct cccgttcctt
      841 tttggctttc cacgcattgg aatttttcag agatattttc gggttgatca atttgtggag
      901 gcgcagataa ttattggcgg tattgggatg gactttaact ccgtaatccc gttgcaggac
      961 gcgcgcgatt ttcactttgc cccaggcgtt gcgcgaatcc ttgcggactt tcagtatcag
     1021 attctcggtt tccttggaaa tccggttgcg cttggatgtg tcatgcgggg cgcgcgaatc
     1081 gggttccagg gagcggggat ctttgcccga ttgctcgaat tcatttttga ttgccgacag
     1141 ccattggcgc gagataccca atttgcgatg agccttggag atattgccgt tttggcgatg
     1201 ataaagctca tacatccaat tcagccgttt cttggcgatg ggcgacagat gatgattctc
     1261 cggatttaac gctttttgga ccattctttg catatccaca aagatagcgt ttcgagcctt
     1321 gttgatcaag ctgtttccgg tttcattttt aaagaaattt ccggccatta ttttttggtt
     1381 cggatactgt caactgtagc ataggccagt gcaggtattg cataaatcgg gtaaaatctg
     1441 ctatcatcta ttaacgccgc ggccggag
//
LOCUS       DAOQWE010000194         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_110824_length_1460_cov_6.080427, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000194 DAOQWE010000000
VERSION     DAOQWE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110824_length_1460_cov_6.080427"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1234
                     /locus_tag="PKK37_04645"
     CDS             179..1234
                     /locus_tag="PKK37_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNR81699.1"
                     /translation="MFSPSYKLTDKMVRMLTEIAESRAIIGRAKILPAQELRLRRQAL
                     IRMTHASTSIEGNMLNLRQVEALCQHKKIDAPARDVYEVQNYLKALRHITKIVEKKHA
                     ISEKVILKIHRLVTDKTLPDEQSGHYRKGMVYVVKRRLGFPDQIIYTAATPQKVPQLV
                     TDLINWIREPQTQEINPVVAAGIVHQEIAAIHPFADGNGRTARVVATLILYQRGYDFR
                     KLFALEDYYNNNRPEYYKAINIGEAYEERRTDFTPWLEYFIKGFKNEIDNTKAQVTAL
                     AHRKIDGKLESQIYLTPEQFKIIDFMEQMGKITVKDVMDILNIPKRTAQLHLQKLKTL
                     KMIAQTGKGPSSAYLLK"
     gene            complement(1275..>1460)
                     /locus_tag="PKK37_04650"
     CDS             complement(1275..>1460)
                     /locus_tag="PKK37_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81700.1"
                     /translation="TSGHEELLLSKHPQGTLIDYTYQVAIHKKWLRAVAAPLLRWYSM
                     KYWEKAVIGRLKKLLEK"
BASE COUNT          451 a          329 c          314 g          366 t
ORIGIN      
        1 aaattttcca tatattccaa atggaatatg ccgccaaaac cggcgcgccg gaatgttatg
       61 tttaaatata gcataaatat aacatttgga attgatattt cttgcgcgat tcttgcgcga
      121 ttcttgcgcg attgttctgg atttgataaa atagaaacat caataccaag aatcaattat
      181 gttcagccct tcttataaac tgaccgacaa aatggtgcgg atgcttactg aaatcgccga
      241 aagccgggcg atcattggcc gggccaagat tttgcccgcc caagagcttc ggttgcgtcg
      301 ccaagcgttg atccgcatga cgcacgcctc aacaagcatt gaaggaaata tgctcaatct
      361 aaggcaagta gaggccttgt gccaacacaa gaaaattgat gcgccggcgc gggatgtcta
      421 tgaagtccag aattatttaa aagcgttgcg gcatataacc aaaatcgttg agaaaaaaca
      481 tgccatttcg gaaaaagtaa tccttaaaat acaccggctg gtcaccgata aaaccttacc
      541 cgatgaacaa tcgggccatt atcgcaaagg catggtttat gtggtgaaaa gaaggttggg
      601 atttcccgac caaataattt acaccgccgc gactccgcaa aaagtgcctc aattggtgac
      661 tgatttgata aattggataa gagaaccgca aacgcaagaa atcaatccgg ttgtggctgc
      721 cgggattgtg caccaggaaa ttgccgcaat tcatcctttt gccgatggca atggccgcac
      781 tgcccgggtc gtggcaactt tgatcttgta tcaacgcggc tatgatttcc gcaaattatt
      841 tgctctggaa gattattaca acaataaccg cccggaatac tataaggcga ttaacattgg
      901 cgaagcttac gaggaacgcc gcaccgattt tactccatgg cttgagtatt ttattaaagg
      961 atttaaaaac gaaatcgata ataccaaggc acaggtaacc gccttggctc atcgaaaaat
     1021 tgatggaaag ttggaatcgc aaatttacct gacgcctgaa caatttaaaa tcatcgattt
     1081 tatggagcaa atgggcaaaa tcaccgtaaa agatgtaatg gatattctca acattccaaa
     1141 aagaaccgcg caactccatt tacaaaaact caagacccta aaaatgatcg cccaaaccgg
     1201 caaaggcccc tcttcggcct atctcctgaa gtaaaatcca aaaatcaggg gttggacccc
     1261 tgtaaatttt gatattattt ctcaaggagt tttttgagcc ggccaatcac cgctttttcc
     1321 cagtatttca ttgaatacca ccgcagcaac ggcgccgcga cggcgcgcag ccatttttta
     1381 tggatggcaa cctgataggt gtagtcaatc aaagttcctt gagggtgttt tgaaagcaac
     1441 agctcttcat gcccggaagt
//
LOCUS       DAOQWE010000195         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_111991_length_1451_cov_5.872493, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000195 DAOQWE010000000
VERSION     DAOQWE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111991_length_1451_cov_5.872493"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKK37_04655"
     CDS             complement(<1..355)
                     /locus_tag="PKK37_04655"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81701.1"
                     /translation="MPKINLSKIIAMAAFSVLALSAGIAGAEETADAPAADDQGSGGP
                     AVLVSPLQGNLTVEDIVGKIAGVIFGLALMICPILIIWGGFEIATAGGDQNKITKGKQ
                     IITFSLVGLAIIALSA"
     gene            complement(361..699)
                     /locus_tag="PKK37_04660"
     CDS             complement(361..699)
                     /locus_tag="PKK37_04660"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81702.1"
                     /translation="MRAISKIFKSIFIFAVLSLPALAGAEKFEFTNPVNKNLDQLISG
                     VMGFLFGVALLVCPILIIWGGFMIATSGGDQEKTKKGKQIITAAVVGLVVIALSNVIK
                     AVIWDVVTAK"
     gene            complement(746..1270)
                     /locus_tag="PKK37_04665"
     CDS             complement(746..1270)
                     /locus_tag="PKK37_04665"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR81703.1"
                     /translation="MASTFINPGLILQQQLQLRRDMIAADFGCGSGEWALALAKILDH
                     GKVWAVDLLEEPLSAARSRAKIAGLQNIEFVKGDIEKLVPRLPADALDLVLMTNLLFQ
                     VGDIGAVFGEAKRVLKPGGKVMVIDWKESARVGPAEKLPPEKVKEVAGHAGFGLTDKF
                     DAGGFHYGMMLEKN"
BASE COUNT          375 a          431 c          300 g          345 t
ORIGIN      
        1 ccgccgacag cgcgatgatc gccaatccga ccagagaaaa cgtgatgatc tgtttgcctt
       61 tggtgatttt gttttgatcc ccgccggcag tggcaatttc aaacccgccc cagatgatca
      121 gaatcgggca aatcatcagc gccagtccga agattacccc cgcaattttg ccgacaatgt
      181 cttccaccgt caagttaccc tgcaacggac tcaccaacac cgccggtccg ccgcttcctt
      241 gatcgtccgc cgccggagca tccgccgttt cttcggctcc ggcaatcccc gccgataacg
      301 ccaaaacaga gaacgccgcc atcgcaataa tttttgataa attgattttt ggcatattat
      361 ttattttgcg gtgacaacat cccaaataac cgccttaatc acattggaaa gcgcgatcac
      421 caccaggccg acgaccgcgg cggtgataat ctgtttgcct tttttggttt tttcctgatc
      481 cccgcccgag gtggcaatca taaacccgcc ccagataatc aggatcgggc aaaccaaaag
      541 cgccacgccg aacaaaaatc ccatcacgcc gctgatcaac tggtcaagat ttttgttgac
      601 gggattggtg aattcaaact tctccgcgcc ggccagcgcc ggcaaagaca gcacggcaaa
      661 gataaaaatg cttttgaata tttttgatat ggcgcgcata ttatgacgaa tttaactcac
      721 cctcaattgg attatcgcaa aataatcaat tcttttcaag catcatcccg tagtgaaaac
      781 cgcccgcgtc aaatttatca gttagtccaa acccggcgtg cccggcaact tccttgactt
      841 tttcgggcgg cagtttttcc gccggcccca cgcgggcgct ttctttccag tcaatcacca
      901 taactttgcc gccgggtttc aacactcttt tggcttcgcc gaacaccgcg ccgatgtcgc
      961 cgacctgaaa cagcagattg gtcatcaaca ccaaatccag cgcatccgcc ggcaaacgcg
     1021 gcaccaattt ttcaatgtcg cccttgacga attcaatgtt ttgcaatccc gcgattttgg
     1081 cgcggcttct cgccgccgac aacggctctt ccagcaaatc aaccgcccaa accttgccat
     1141 gatcaagaat ttttgccagc gccagcgccc attcccccga cccgcaaccg aaatccgcgg
     1201 caatcatatc gcgccttaat tgcaattgct gttgcaaaat cagcccggga tttataaatg
     1261 tagacgccat aaaaatgtaa aattgaaaaa tcaaaaatca attcttcgga aagctcagaa
     1321 ttgatgctga gcaagccgaa gcatcaaggc gataaaacaa gaatcttatt ctccaatttt
     1381 aacctgcggt tgatttagat gcaattggcc gacgcgactt tgtcgccgtc ttccaggcgc
     1441 ataatgcgca c
//
LOCUS       DAOQWE010000196         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_113548_length_1440_cov_2.119134, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000196 DAOQWE010000000
VERSION     DAOQWE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113548_length_1440_cov_2.119134"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1324)
                     /locus_tag="PKK37_04670"
     CDS             complement(188..1324)
                     /locus_tag="PKK37_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81704.1"
                     /translation="MNYIRTIFDDICKNIGKKPILVLYGARQVGKTTLVKSVMAKFKN
                     PLYLQGDDPKDALLIEGRSGRELVDIISGHDLTVIDEAQKVKDIGVVLKLIADNKKDA
                     QIIATGSSSFELANKVSEPLTGRNRKFYLYPLSVKELVQACGARVIKNNLEEYLAFGS
                     YPQIANAKTRQEKISLVKELAGDYLFKDLFLFGGIRNPFTFEKLVKLLALRVGSEISY
                     SELAKEAGISRGTVLNYVTLLEQAFIAFRLRPLYTNKTKEINKSHKIYFYDVGIRNAL
                     IGNTDPIELRPDKGAIFENFFIAEMIKDRAYSGRNSEINFWRDRQGAEIDLVESSNAG
                     KVIAAYECKWKETAAMPAPFKNLYPRATFTAITASNIVDQLALT"
BASE COUNT          374 a          346 c          277 g          443 t
ORIGIN      
        1 ttatctttgt cggccttaac tttttgcccc tcgcttattt ttgttttttt cattgaattt
       61 tgataaacct cgtttgtatt attgattata ccgattattc gcccgttcac caataaccag
      121 tggtacgctt tcgtcggtgt cagccaaaat ctggccgaaa ttgaacgttc tacaggtcca
      181 aaataattta agtgagcgct agctgatcaa caatgtttga cgcggtaatc gcggtaaatg
      241 tcgcccgcgg atataaattt ttaaatggtg cgggcattgc ggcggtttct ttccatttgc
      301 attcataagc ggcaatgacc ttgcccgcat ttgacgattc caccaaatca atttccgcgc
      361 cttgccgatc gcgccaaaaa ttaatctcgc tgttccgtcc ggaataagcg cggtccttta
      421 tcatttcggc gataaaaaaa ttctcaaaaa tcgccccctt gtccgggcgc aattcaatcg
      481 gatcggtgtt cccgataagc gcgttgcgta tgccgacatc ataaaaatat attttgtggc
      541 ttttgttgat ttccttggtt ttgttggtat acagcggccg caagcgaaac gcgataaacg
      601 cttgctcaag aagtgtaaca taattaagaa ccgtgccgcg gctgatgcct gcctctttgg
      661 caagttccga ataagatatt tcactgccaa cccgcaacgc caacaacttg accagctttt
      721 caaaagtgaa cggattgcgg attcccccga acaaaaacaa atctttgaat aaataatctc
      781 cggccaattc tttgaccagc gatatttttt cttggcgggt tttggcattg gcaatttgcg
      841 gatacgaccc aaaagcgaga tactcttcaa gattgttttt tataacgcgc gcgccgcatg
      901 cttgcaccaa ttctttcacg gacaacggat acaaataaaa cttgcgattc ctgccagtga
      961 gcggttcact cactttgttt gccaattcaa aagaagacga tccggtcgca ataatttgag
     1021 cgtctttttt gttgtcggca atgagtttca gaacaacgcc gatatcttta actttctgtg
     1081 cctcgtcaat aactgtcaaa tcatgtccgg aaattatgtc cacaagttcc cgtcccgacc
     1141 ggccttcaat gagaagtgca tctttggggt catcgccttg aagatataat ggatttttga
     1201 attttgccat taccgacttt accagcgtgg ttttgccaac ctgtcgcgcc ccgtataaaa
     1261 ccaatatcgg ttttttgccg atatttttgc aaatatcatc aaatatcgtg cgtatataat
     1321 tcatatcatt attatacaaa atatatactc aaaatgcaat aataatcgca ttttatgaac
     1381 aattcctcaa actttctgcc agccgtcgtc gccttgcccg ccctcaacct cgttgacatc
//
LOCUS       DAOQWE010000197         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_114411_length_1433_cov_4.469521, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000197 DAOQWE010000000
VERSION     DAOQWE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114411_length_1433_cov_4.469521"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PKK37_04675"
     CDS             <1..295
                     /locus_tag="PKK37_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81705.1"
                     /translation="PIAGIMVLAIGYFLPLRCYSLIRRLSRGTFAFFLYHYLIILFVF
                     SYLKLNIVGYHISADFIVLYLIMLVGFSIFQSFFDKIIGKKIKKLSEKEYIIN"
     gene            303..896
                     /locus_tag="PKK37_04680"
     CDS             303..896
                     /locus_tag="PKK37_04680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNR81706.1"
                     /translation="MIKRMIDILGSGFGLVILSPVFLWVALKIKLDDGGPIFYKGRRV
                     GKGGKLFKMYKFRTMVIGADKLGGPSTAADDPRLTKIGLFLKKYQLDELPQLINVFKR
                     EMSLVGPRPEVEMYVDMMTAEQKNAILSVRPGMTDYASLWNFHESEILKGAKDPEIAY
                     QKLIRPKKLALQMRYVKERSLWVDIKIIFLTIFKIFK"
     gene            918..>1433
                     /locus_tag="PKK37_04685"
     CDS             918..>1433
                     /locus_tag="PKK37_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HNR81707.1"
                     /translation="MNKPDYKKIANDARIRVLEMIYKAQTSHIGSNYSCIDLLAVIFE
                     KADLNKDKVVLSKGWAAASLYYFLKEKGRITETDLESYCQTGSKFIGLAEPIIPDIPA
                     AGGSMGFGLPFGVGFALAKKTKKEAGKIFVLMSDGEMQIGTTWESALIAAHHKLDNLL
                     VFVDVNGLQAMG"
BASE COUNT          403 a          236 c          345 g          449 t
ORIGIN      
        1 gcctatcgcg ggaattatgg tgctggcgat cggttatttt ttacctttac ggtgctattc
       61 tttaatcaga cggctttcaa gaggcacttt tgcttttttc ctttatcatt atttaattat
      121 cctttttgtt tttagctatc ttaaattaaa tatagtcggt tatcatatct cggctgattt
      181 tatcgtctta tacctgatta tgttagttgg attttctatc tttcaatctt tttttgacaa
      241 aattattggt aaaaaaatta aaaaattatc cgaaaaagag tacataataa attgataaaa
      301 caatgattaa gaggatgatt gatattttgg gatcggggtt cgggctggta attttgtcgc
      361 cggtgttttt gtgggtggcg ttgaaaatca aattggacga tggcgggccg attttctata
      421 agggtcggcg tgtcggcaaa ggtggcaaat tattcaaaat gtataaattc cgcacaatgg
      481 tgatcggcgc cgataagctt ggcggtcctt ccactgccgc cgatgatccg cggttaacta
      541 aaatcggatt gtttttgaaa aaataccaat tggatgaatt gccgcaatta atcaatgtct
      601 ttaagaggga aatgagtctt gtcgggccgc gtccggaagt tgagatgtat gttgatatga
      661 tgacagccga acaaaaaaat gctattttat cggtgaggcc gggaatgacc gattatgcgt
      721 cgctttggaa ttttcacgag agcgaaattt taaaaggcgc caaggatccc gagattgctt
      781 atcaaaaact gattcgtccc aaaaagttgg ctttgcagat gagatatgtc aaagagcgct
      841 cgctttgggt tgatataaaa attatctttt tgacgatatt taagattttt aaatgaattg
      901 ataaccatat cattttaatg aataaaccag attacaaaaa aatcgccaat gatgcccgaa
      961 tccgggtttt ggagatgatc tataaagcgc aaaccagcca tatcggcagt aattacagtt
     1021 gcattgattt gctggcggtg atttttgaaa aagccgattt aaataaagac aaagtggttt
     1081 taagtaaggg ttgggcggcc gcgtcgcttt attattttct caaagaaaag gggaggatca
     1141 ccgaaacgga tttggaaagt tattgccaaa cggggagtaa gtttatcggg ttggccgagc
     1201 cgataattcc cgatattccc gccgcgggcg gctcaatggg gtttggtttg ccgtttggag
     1261 tcggttttgc cttggccaaa aaaaccaaga aagaggcagg aaaaattttt gtgttaatgt
     1321 cggacggcga aatgcaaatc ggcaccactt gggaatcggc gctgattgcc gctcatcaca
     1381 aattggacaa tcttttggtg ttcgtggatg ttaatggttt gcaggccatg ggc
//
LOCUS       DAOQWE010000198         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_115947_length_1422_cov_7.732992, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000198 DAOQWE010000000
VERSION     DAOQWE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115947_length_1422_cov_7.732992"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..146)
                     /locus_tag="PKK37_04690"
     tRNA            complement(72..146)
                     /locus_tag="PKK37_04690"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Ile,seq:gat)
     gene            complement(167..760)
                     /locus_tag="PKK37_04695"
     CDS             complement(167..760)
                     /locus_tag="PKK37_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNR81708.1"
                     /translation="MTKIKSKANAKTGTGKEIKIPKKVAGFLAKVGAKYELIGHRTVY
                     TAFDKAATLRVKLGAVVKVLTLKIGKELALAVIGGDKNLDIEKLQKLAKNEKIAFAKE
                     KTIAENFKGIDPGAISPFENLWKMKVFCDKKLLESSKIIISAGSYEASIIITPAAFKK
                     LNPQMTIANFSALRPKPKAKAIKPKSANKPALKKKRK"
     gene            complement(764..943)
                     /locus_tag="PKK37_04700"
     CDS             complement(764..943)
                     /locus_tag="PKK37_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNR81709.1"
                     /translation="MEKNGFVFASQNGSHAKYRKNGAPNLTAIVPIHGKEILYGTFRS
                     IMRQSQLSESKFRNS"
     gene            complement(975..1193)
                     /locus_tag="PKK37_04705"
     CDS             complement(975..1193)
                     /locus_tag="PKK37_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNR81710.1"
                     /translation="MKRVNLKAAVWKEGKQYVSQCLNVDVASFGATKKKAMENLKEAL
                     ELYFEDQDISQINKVERPDVFSLTLTNA"
     gene            1224..1358
                     /locus_tag="PKK37_04710"
     CDS             1224..1358
                     /locus_tag="PKK37_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81711.1"
                     /translation="MAPSYQNSPFRQLFVAIICLCYCQCGHPCAKRDYLLDINYSGVW
                     "
     gene            1346..1417
                     /locus_tag="PKK37_04715"
     tRNA            1346..1417
                     /locus_tag="PKK37_04715"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1379..1381,aa:Trp,seq:cca)
BASE COUNT          328 a          322 c          281 g          491 t
ORIGIN      
        1 acgcaaacac aactatacaa taacaaaaag gcattaattt acaagaaaac cgctctttgg
       61 cggtttcctt ttggtgggcg cggaaggaat cggaccttcg gcctctgtct tatcaggaca
      121 gcgttatacc acttaactac gcgcccacaa acatattgtt tataccctat ttcctcttct
      181 ttttcaaggc gggcttattg gcgctcttgg gttttatcgc tttcgctttc ggcttgggtc
      241 gtagcgcgga gaagttggcg atagtcattt ggggatttag ttttttgaag gcggcgggcg
      301 tgataattat tgatgcttca tagcttccgg ccgagataat gatttttgac gattccagca
      361 gttttttatc gcagaaaact ttcattttcc acagattctc aaagggtgaa atcgcgcccg
      421 gatcaatgcc tttaaaattt tcggcaattg tcttttcttt ggcaaaggcg attttctcgt
      481 tttttgcgag tttttgcagt ttttcaatat ccagattctt gtcgccgccg attaccgcta
      541 acgccaattc tttgccgatt ttaagcgtta aaacttttac cactgcgcca agcttcactc
      601 ttaaagtcgc cgccttgtca aaggcggtgt agaccgtgcg atgcccgatc aattcatatt
      661 ttgcgccaac cttcgccaaa aaccccgcca cctttttcgg aatttttatt tcttttcctg
      721 ttcctgtttt tgcattggct tttgatttta tctttgtcat tttttaagaa ttgcgaaatt
      781 tactttcaga caattgcgat tggcgcatta ttgaacgaaa agttccgtaa agaatttcct
      841 tgccgtgaat cggcacgatt gccgtcaaat tcggcgcgcc gtttttgcga tatttggcgt
      901 gactgccgtt ttgagaagca aaaacgaaac catttttctc caaagttttg ataaccaatt
      961 ttgtttgtat cggattaggc attggttaaa gtaagcgaga aaacatccgg ccgttccact
     1021 ttattgattt gggaaatatc ttgatcttca aaatacaact ccagtgcttc tttgaggttt
     1081 tccattgctt tttttttggt cgcaccgaaa ctggccacat caacattgag gcattgcgaa
     1141 acatattgct tgccttcttt ccaaaccgcg gcctttaaat tgaccctttt cattgtttta
     1201 attttataat tgaattttaa cctatggctc catcctacca aaattctccc ttccgtcaat
     1261 tgtttgttgc cattatttgc ctctgttatt gccagtgcgg ccatccttgc gccaagcgtg
     1321 attatttgct agatattaac tattcggggg tgtggtgaaa ttggcatcac aaaggtctcc
     1381 aaaaccttcg tttcaggttc aagtcctggc acccccgctt tc
//
LOCUS       DAOQWE010000199         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_116488_length_1418_cov_4.736610, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000199 DAOQWE010000000
VERSION     DAOQWE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116488_length_1418_cov_4.736610"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1418)
                     /locus_tag="PKK37_04720"
     CDS             complement(<1..>1418)
                     /locus_tag="PKK37_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNR81712.1"
                     /translation="PHNLGEVCDALTYLLDAPKATTEDLFEFIKGPDFPLGGEIFDRK
                     EIVAAYSQGKGPIVCRGKAEIVQNEKTDRYQIVITEIPYQVQKSGLLEQIAKLVSEKK
                     IDGVKDVRDESDKDGLRVAIDLNRDAQPQKILNRLYKFTDLQRTFHLNMLALVDGIQP
                     QVLSLAETLNYFLEHRKQVVARRTQFELDRAKERAHILEGLVRCLADIDEVIRIIRKS
                     ENREDARHNLMKRFKLDEIQANAILETKLAALAKLERQKIDNELKELEAKIAEYLGIL
                     KSPAKIKAVIKKELMKAKETYGDERRTKVCVHKIGEISEADLIPDEEVAITLTNSGYI
                     KRIDPKTYKIQKRGGKGIIGLKTVGDDIVEQSLIANTHDRILFFTDMGRVFQTLAYEI
                     PEGARVAKGRALVNFLDLGAQEKVLYLMSLGSASAKTMAGKNKSKANGNGTAEESKYL
                     LIVTKNGIAKKTATEDFDNIRR"
BASE COUNT          292 a          383 c          342 g          401 t
ORIGIN      
        1 aacggcggat attgtcaaaa tcttccgtcg cggttttttt ggcaatgccg tttttggtca
       61 caatcaacag atatttgctt tcctcggcag tgccgttgcc gttggctttg gatttgtttt
      121 tgcccgccat agttttggcg gaagcggatc ccagcgacat cagataaagc actttttctt
      181 gcgcgcccaa atccaaaaaa ttcaccagcg cccgcccttt ggccacgcgc gctccctccg
      241 gaatttcata ggccaaggtt tgaaaaaccc gccccatatc ggtgaagaac aatatccggt
      301 cgtgcgtatt ggcaatcagc gattgctcca cgatgtcgtc gcccacggtt ttgaggccga
      361 taatgccttt tcccccgcgc ttttgaattt tgtaggtttt gggatcaatg cgcttgatgt
      421 agccggaatt ggtcagcgtg atcgccactt cttcgtcggg aatcaaatcg gcttcggaaa
      481 tttcgccgat cttgtgaacg cagacttttg tgcgccgttc atcgccgtag gtttctttgg
      541 ccttcatcag ttcttttttg atcaccgcct tgatcttggc cggacttttt aaaatgccga
      601 gatattcggc gattttggcc tccaattcct tcaattcatt gtcaattttc tgccgttcca
      661 gtttcgccag cgccgccagt ttggtttcca aaatcgcatt ggcctggatc tcgtcaagct
      721 tgaaccgctt cattaaattg tggcgcgcgt cttcgcggtt ttcggatttt ctgatgatgc
      781 ggatcacctc gtcaatatcg gccaggcatc tcaccaaccc ttccaaaata tgcgcccgct
      841 ctttggcgcg gtcaagctca aattgggtgc ggcgcgccac cacttgcttg cgatgctcca
      901 aaaaataatt cagcgtttcg gcaaggctca aaacctgggg ctggatgccg tccaccagcg
      961 ccagcatatt caaatggaag gtgcgctgga gatccgtgaa tttgtaaagc cggttgagaa
     1021 ttttttgcgg ctgggcatcg cggttaaggt caatcgccac gcgcaggccg tccttgtcgg
     1081 attcgtcgcg gacatctttg acgccgtcaa tctttttttc cgataccaat ttggcgatct
     1141 gctccaacaa tcccgacttt tgcacctgat aaggaatctc ggtgatgacg atctgatagc
     1201 gatcggtctt ttcattttgc acgatctcgg ccttgccgcg gcaaacgatc ggccccttgc
     1261 cttgcgaata ggcggcaaca atctctttgc ggtcaaaaat ttctccaccc aaaggaaaat
     1321 cggggccctt gataaactca aacagatctt cggtggtcgc cttgggggcg tcaagcagat
     1381 atgtgagcgc atcgcaaact tcacccaaat tatgcggc
//
LOCUS       DAOQWE010000200         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_116809_length_1416_cov_2.386481, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000200 DAOQWE010000000
VERSION     DAOQWE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116809_length_1416_cov_2.386481"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PKK37_04725"
     CDS             complement(<1..1160)
                     /locus_tag="PKK37_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81713.1"
                     /translation="MFSFLTIALFLFAYSWAQELPEIIIEDADATTTIDLVSPPTDFF
                     DAGEVAFRLPNQALVCRADEEEVINLTAPPEDFFDASEVDLRLPNEAAVCRADETFTI
                     DLVAPPAEFFNPAPDFVDKFVYVALGDSYQSGEGAGNSISNSADYLSQAYEVGTYTDI
                     LVAGDNSCHRALKNYAKINKNKFRPELSDDDIVLIDFTCSGAKIENGQRPPIVGEMTS
                     NQIAPESQLQQALNKLTELNLTPADVDLVTVGMGGNDAKFTEIIHACVLPSILRRALQ
                     EYPDSPPEIEFLTDQFATCENFDRFGANSQNAINQLYAKEFWAQGKILQTFANARVLQ
                     LNYPNILPEKEKSPAWCSGIRKEDINYARKKVKDINKKIAEAFNNYAASSRF"
BASE COUNT          381 a          346 c          310 g          379 t
ORIGIN      
        1 tcaaagcgcg aactggcggc ataattatta aatgcctcgg caattttttt gttaatatct
       61 ttcacctttt tgcgcgcgta attgatatct tctttgcgaa tgccgctaca ccatgccggt
      121 gatttttctt tctcgggcag aatattggga tagtttaatt gcaaaacacg ggcattggca
      181 aaggtttgca aaatttttcc ctgcgcccaa aactccttgg cataaagttg attgatggca
      241 ttttgactgt ttgcgccaaa acgatcaaag ttttcgcaag tggcaaattg atcggtcaaa
      301 aattcaattt ccggcggcga atccggatat tcttggaggg cgcgccgcaa aatactcggc
      361 agaacacaag catgaatgat ttccgtaaac ttggcgtcgt tgccgcccat gccgacagtg
      421 accagatcaa catcggcggg agtcagatta agttcggtaa gtttgtttag cgcttgttgc
      481 agttgcgatt cgggcgcgat ttggttgctt gtcatttcgc ccacaatcgg cgggcgttgt
      541 ccgttttcaa ttttggcgcc gctgcaagtg aaatcaatta aaacaatatc atcatcgctc
      601 aactcgggac gaaatttgtt tttgttgatt ttggcatagt ttttcagggc gcgatggcaa
      661 gaattgtcgc cggcgaccaa aatgtcagta tatgtgccaa cctcatatgc ctgcgataga
      721 taatcggcag aatttgaaat ggaattgccc gcgccctcgc cggattgata ggaatccccc
      781 agcgccacat aaacaaactt gtcaacaaaa tccggcgcgg gattaaaaaa ttccgcgggc
      841 ggggcaacca aatcaatcgt aaaagtttca tccgcgcggc aaaccgccgc ttcgttgggc
      901 aaacgcagat caacttccga cgcatcaaaa aaatcttccg gcggagcagt taaattgatc
      961 acctcttctt cgtcagcgcg gcaaaccaac gcttgattgg gcaagcgaaa cgctacctcg
     1021 ccggcatcaa aaaaatccgt gggcggagaa accaaatcaa ttgtggtggt tgcgtcggca
     1081 tcctcaatga taatttcggg caattcctgc gcccaagaat aggcaaacaa aaacaacgca
     1141 attgttaaaa atgaaaacat tggcaaatat cgcttcataa attgaaaaat tttaatctgg
     1201 caaagtttga ttttaatctg gtgaggtttg acctcaccac tattacctcg ccgtccgcgt
     1261 caatccgcct aattcattct acccttcggg cgaaccatct catcaatacc aactctattt
     1321 caaacattca gagggtctaa ccctctgatc ttcccgtctc attgaaagtt tttgaaatta
     1381 ccacaccggc ttgggatcgc ttaccccgcc ctcctc
//
LOCUS       DAOQWE010000201         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_117017_length_1414_cov_3.183223, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000201 DAOQWE010000000
VERSION     DAOQWE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117017_length_1414_cov_3.183223"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1158
                     /locus_tag="PKK37_04730"
     CDS             151..1158
                     /locus_tag="PKK37_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR81714.1"
                     /translation="MQTPSEKTEKILLATEEEVFKMIIGAAHPFRKLNELIDFEKLVE
                     PMRATYSTLGANGIDVKKGIKALLIQFWEDYSDREMEQAIKENLAVRWFCGFSLTEAT
                     PDHTYFCKLRKRIGTKRVGQFFKDVNAILDGYGLFGNVFTFIDASTIITKTALWEERD
                     QAIKDGADKLNNSNVKDYAVDKDARWGAKSKNHIWFGYKRNTAVDMRCGLIKKVAITP
                     ANVPDFKAAGNVMPNQGAVFMDKIYDTKKTDALVKAHGCYPATIRKNNNKNKDRDLDS
                     WRSKVRMPFEGTFSKMDKHARYRGQAKNAMQGFLQAIAFNLKKAINILTVLPSSTATA
                     A"
BASE COUNT          458 a          306 c          317 g          333 t
ORIGIN      
        1 tttttcaaga attaaaggaa catttgactc aaaaggaaat ttctttgttg gttggggcct
       61 gttgccgaat ggcgaatatt tgaaggattt tcgagcgtga atatggtaaa attgaattaa
      121 ggcaaagcct taatctctaa ttgaaaaacc atgcaaacgc cctctgaaaa aactgaaaaa
      181 atcttgcttg caaccgaaga agaagttttt aaaatgatca ttggcgcggc gcatccgttc
      241 cgcaaattaa acgaacttat tgattttgaa aaactggttg agccaatgcg cgcaacctat
      301 tcgacgctgg gagccaatgg tatcgatgtc aaaaaaggaa tcaaagcgtt gctgatccaa
      361 ttctgggagg attattccga ccgggaaatg gaacaggcga tcaaagaaaa tctggcggta
      421 cgctggtttt gcggtttttc gcttaccgaa gcaacgcctg accacaccta tttctgcaaa
      481 ttgcggaaac ggatcggaac caaaagagtc ggccaatttt tcaaagatgt gaacgcgatt
      541 ttagacggtt acggcctttt cggcaatgtt ttcactttta tcgacgcgtc cacgatcatt
      601 accaaaaccg ctctctggga agagcgcgac caagcgatta aagacggtgc ggacaaattg
      661 aacaattcca atgtcaaaga ctacgccgtc gacaaagacg cccgttgggg cgcgaaatca
      721 aaaaaccata tctggtttgg ctacaaaaga aacaccgcgg ttgatatgcg ctgcggatta
      781 atcaagaaag tggcaatcac tccggccaac gttccggatt ttaaagccgc cggaaacgtt
      841 atgcccaatc aaggcgcggt atttatggac aaaatctacg acaccaagaa aaccgatgcc
      901 cttgttaaag cgcacggatg ttatccggcc acaatccgca aaaacaacaa caaaaacaaa
      961 gaccgcgatt tggacagctg gcgatcaaaa gtacgtatgc cgtttgaagg tacattttcc
     1021 aagatggaca aacacgcccg ctatcgcggg caagcaaaaa atgcaatgca gggatttttg
     1081 caagccattg ctttcaacct caaaaaagcg atcaatattt taacagtttt gccgtcttcg
     1141 accgccaccg cggcttagca agggcagagt gcgcctgcgg agcgcaaaaa caacaatcag
     1201 atgattgaaa aatcaaaaac agccgcaaac taaaagaaaa agcaaagtta tcaacaagta
     1261 gtttcgttaa tagcttcgct tttcctttta aaggttaatt tagcaacaaa cccggttggt
     1321 ccgcggcaag tcggcaaaac cacattgatg cgcgaattgg tcgcgcatct tgaaagtatc
     1381 ggtaaaaagt atattttttt aagtttggac aatg
//
LOCUS       DAOQWE010000202         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_117948_length_1408_cov_1.591279, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000202 DAOQWE010000000
VERSION     DAOQWE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117948_length_1408_cov_1.591279"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKK37_04735"
     CDS             <1..745
                     /locus_tag="PKK37_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81715.1"
                     /translation="KVFINYADQVFGPYAPDENGGVLIEVSLLDQAGVVKIEAQSETT
                     GKIVEEAIEVADKTPPEIFIISPRKDQDYINNQILLLDFRVIDNQSEELPLKTKIFLN
                     GDAMFQNAIDLSLQKTGNHRLEIVAEDEGGNIGRTELDFANIATVNSIIANISHYYDL
                     KVIKLLSARNTLLAAAKSLKLQLDALGKLKQSALPPATKNYLITLAQNSINRQIDAMI
                     ALIGKLPAISIDASAKELLIDSLRYIKIK"
     gene            757..1275
                     /locus_tag="PKK37_04740"
     CDS             757..1275
                     /locus_tag="PKK37_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81716.1"
                     /translation="MNKKNKILVAVVSILLAVVSLWHFFADDLFIFVLSRAVPPGEPM
                     PKEQLMPCDGNPASFVELGSPYLTNPTAEFATAGGEIYVIARRYEHGILLPNRGGSGI
                     YIGFLNQPPTWNKQTSEITNIYKEIVIKEKDYGTFDLPAGRYWLWSSSGGDVVVYSCE
                     EGGVSDPKPVWR"
BASE COUNT          418 a          271 c          340 g          379 t
ORIGIN      
        1 aaaagtgttt atcaattacg cggatcaagt ttttggtccg tatgcccccg atgaaaatgg
       61 cggcgtgttg attgaagtgt ctttacttga ccaagccggt gtggtgaaaa ttgaagcgca
      121 aagcgaaacc accggcaaaa ttgttgaaga agctattgag gttgccgata aaacgccgcc
      181 ggaaattttt atcatttctc cccgaaaaga ccaagattac attaataacc aaattctgtt
      241 gctggatttt cgcgtcattg acaatcaatc cgaggagctt cccctgaaaa ccaagatttt
      301 cttgaacgga gatgccatgt ttcaaaacgc cattgattta tcgctacaaa aaaccggcaa
      361 ccatcggctt gaaattgttg ccgaggacga aggcggcaat atcggccgca ctgaattgga
      421 ttttgccaac atagcgactg ttaattcaat cattgccaat atcagtcact attacgacct
      481 caaagtaatt aaacttttat cggcgcgcaa cacgctgttg gcggcggcaa aatcattgaa
      541 acttcaactt gacgcgctgg gcaaattaaa acagagcgcc ttgccgccag caacaaaaaa
      601 ttatttaata acattagcgc aaaattcaat taaccgccag attgacgcaa tgattgcttt
      661 gatcggtaaa ttgccggcaa tatctattga cgcgtccgcc aaagaacttt tgattgacag
      721 cttgcgatat attaaaatca aataaattaa aataaaatga acaaaaaaaa taaaattctt
      781 gttgccgttg tttcaatctt gctggcagtt gtttctttgt ggcatttttt tgccgacgat
      841 ttgtttatct ttgttctttc gcgcgccgtg ccgccgggcg agccgatgcc caaagaacag
      901 ttaatgccct gtgacgggaa tcccgccagc ttcgtggagt tgggttcgcc atatttaacc
      961 aatcctaccg cagaattcgc cactgccggc ggagaaattt atgttatcgc acggagatat
     1021 gaacacggaa ttttattgcc taatagaggg ggttccggta tttatattgg ctttttgaat
     1081 caaccgccga catggaataa acaaacaagc gagataacta acatttataa agagattgta
     1141 atcaaagaaa aggattatgg tacatttgat ttaccggcgg ggagatattg gctgtggtca
     1201 agttcgggcg gggatgttgt ggtttatagt tgcgaggaag gcggggtgag tgatcccaag
     1261 ccggtttggc ggtgaagggt ggcggagcag atgaataaaa tgaataaaaa atgagggagg
     1321 ggggattcag agggttagac cctctgaatg tttgaaatag agttggtatt gataggatgg
     1381 ttcgcccgaa gggtggaata aatcggat
//
LOCUS       DAOQWE010000203         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_118220_length_1405_cov_8.511111, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000203 DAOQWE010000000
VERSION     DAOQWE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118220_length_1405_cov_8.511111"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1246
                     /gene="tgt"
                     /locus_tag="PKK37_04745"
     CDS             23..1246
                     /gene="tgt"
                     /locus_tag="PKK37_04745"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNR81717.1"
                     /translation="MADFKFKILAHDENSRARAGVIRTRRGDIDTPHFVPVGTLASVR
                     ALDSADLESLGAQCALVNTYHLHLKPGDKLIKKLGGTHKFMNFGKPLFSDSGGFQAFS
                     LGLAREHNIGKIGVFPAESKNTGVGPLSNLPGSNPCKNLAKVTGAGIEFKSVYDGSRH
                     FFDAKKSMEIQSNLDTDIIMAFDECTSPLSDYGYTQTAMIRTHRWAEQSLKYHNKDQA
                     IYGIVQGGYFNDLRDESTQFINSLPFDGIAVGGSLGKTKADMHEILEWVIPQLDGRPR
                     HLLGIGEVDDIFECVARGIDTFDCVGPTRIARRANLYVLPASGGNGKNKFRINIAAAK
                     YAGDPGPVDANCRCSVCENYSRAYLRHLYEARELAYFRLATIHNLWFILRLMEKIRES
                     IKNGNFAELKKEWLG"
BASE COUNT          349 a          313 c          385 g          358 t
ORIGIN      
        1 aagcttccta agtttgtgaa aaatggccga ttttaaattt aagattttgg cgcacgatga
       61 aaattcgcgg gcgagagcgg gggtgattcg cacccgtcgc ggcgacattg atacaccgca
      121 cttcgtgccg gtggggactt tggcgtcggt gcgggcgttg gattcggcgg atttggaatc
      181 cctgggcgcg caatgcgcgc tggtgaacac gtatcatttg cacttgaagc ccggcgacaa
      241 attgatcaaa aaactgggcg ggacgcacaa atttatgaat ttcggcaagc cgctgttttc
      301 cgattcgggc gggtttcagg cattttcttt gggattggcg cgggagcaca atatcggcaa
      361 gattggcgtt ttccctgccg aatctaaaaa tacaggggtt ggacccctgt caaatttacc
      421 ggggtccaac ccctgcaaaa atttagccaa ggtgaccggc gcgggaattg aatttaagtc
      481 ggtctatgac ggatcgcggc acttttttga tgccaagaag tcaatggaaa tccaatccaa
      541 tctggatacc gacatcatca tggcgtttga cgaatgcact tcgccgcttt ctgactatgg
      601 ctatacccaa accgcgatga tccgcacgca tcggtgggcc gaacaatctt tgaaatacca
      661 caataaagat caggcaatct acggcatcgt ccaaggcggt tatttcaatg atttgcgcga
      721 tgaaagcacg caatttataa attccctgcc gtttgacggc attgccgtcg gcgggtcctt
      781 gggcaaaacc aaagcggata tgcacgaaat tttggaatgg gtgattccgc aattggatgg
      841 ccggccaaga caccttttgg gtatcggcga ggttgacgat atttttgaat gcgtggcgcg
      901 cggcattgat acctttgatt gcgtcggccc gacgcgcatc gctcgccgcg ccaatctgta
      961 tgttctgccc gcctccggcg gcaatgggaa aaataagttc cgcatcaata ttgccgctgc
     1021 caaatacgct ggcgatccgg gtccggttga cgccaattgc cggtgctccg tgtgcgaaaa
     1081 ttattcgcgg gcgtatttgc ggcatctgta tgaagcgcgc gaacttgcct atttccgttt
     1141 ggcgacgata cacaatctgt ggtttatcct gcgcctgatg gaaaaaatac gggaaagcat
     1201 caaaaatggg aatttcgccg agctgaaaaa ggaatggctt ggttaagtgg acaaaagcgg
     1261 gaaattttgc tattaaattg gtgtttggat gaaattggaa atgtaaaatt tagaaattgg
     1321 cggcttctat ttttaattgc aatttttagt ttgaaacgcc gaggtagcca agtcggtcac
     1381 ggcgaatgtc tgaaaaacat tagat
//
LOCUS       DAOQWE010000204         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_120948_length_1386_cov_6.335086, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000204 DAOQWE010000000
VERSION     DAOQWE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120948_length_1386_cov_6.335086"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PKK37_04750"
     CDS             complement(<1..384)
                     /locus_tag="PKK37_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81718.1"
                     /translation="MAVLPVAGGAAGGLISFYQCTQLAPLYLPSMLSSPKIEKSETVI
                     QENKALKNAIAKVKNMAIAVKTGDGRTVSGAAITSDGLAVTLNSAYPAGAAAEVFSAG
                     EKIGFEVVKRDKEANLAILRLSGADL"
     gene            complement(425..>1386)
                     /locus_tag="PKK37_04755"
     CDS             complement(425..>1386)
                     /locus_tag="PKK37_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR81719.1"
                     /translation="LEEILKRRVIGQEAAIGAIANAIRRARAGIAEESRPLGSFMFLG
                     PTGVGKTETAKALAGFLFNDENALVRLDMSEYMEKHTVSRILGSPPGYVGYEEGGQLT
                     EKIRRRPYSVILLDEIEKAHPEVFNILLQILEDGRLTDAKGRTASFKNAILIMTSNIG
                     SDVIAKEAALGFSARPEAENGKEKIQEKVMAVLKESFRPEFLNRVDEIVIFNYLGSAE
                     IKKIVDLELQKVDRRLAAKNIRLAVADAAKELLARRGFDANLGARPLRRVIQKLVLDA
                     LALKIVSGEVREGDCVSVEVKNDEIAVIAPARQTLRRPAKVAV"
BASE COUNT          301 a          431 c          322 g          332 t
ORIGIN      
        1 taaatccgcg ccgcttaaac gcaaaattgc caaattcgcc tccttgtcgc gcttcaccac
       61 ttcaaagccg atcttttcgc cggcggaaaa aacttccgcc gcggcgcccg ccggataggc
      121 actgtttagc gttaccgcca gtccgtcaga ggtgatcgcc gcgccggaaa ccgtgcggcc
      181 atcgccggtc ttgacggcga tggccatatt tttcactttg gcaatggcat tcttcaacgc
      241 tttgttctct tgaatcaccg tttcgctctt ctcaattttt ggcgatgaaa gcattgacgg
      301 caaataaaga ggcgccaact gggtgcattg atagaaagaa atcaatcctc ccgcggcgcc
      361 gccggcgacc ggtaacacgg ccattgcaac gattttcaga atttttttga attttatcat
      421 cggtttaaac cgccactttc gccggccggc gcaaagtttg gcgcgcgggc gcgatcacgg
      481 caatctcgtc gtttttaacc tccaccgaaa cgcaatcccc ttcccggact tcgccggaaa
      541 cgattttgag cgccagcgcg tccaacacca gtttctggat tacccgccgc agaggccgag
      601 cgcccagatt agcgtcaaat ccccgccgcg ccaacaattc tttggccgca tcggccaccg
      661 ccaagcggat attttttgcc gccaaccgcc ggtccacttt ttgcaattcc agatcaacga
      721 tctttttgat ttccgcggat cccaaatagt tgaagattac aatctcatcc acgcgattca
      781 aaaattcggg gcgaaaagat tctttcagca ccgccatcac tttttcctga attttttctt
      841 tgccgttttc cgcttccggg cgcgcggaaa atcccagcgc cgcctccttg gcaatcacgt
      901 ccgaaccgat gttggaagtc ataatcaaaa tcgcgttctt aaacgatgcc gtgcggcctt
      961 tggcgtcggt caaacggccg tcttccaaaa tttgcaacaa aatattgaaa acttccggat
     1021 gggctttttc aatctcgtcc aacaatatca ccgaataggg acggcgccgg attttttccg
     1081 tgagctggcc gccttcttcg tagccgacat agccgggcgg cgaacccaaa atccgcgaca
     1141 cggtgtgctt ttccatatat tcggacatat ccaaccgcac cagcgcgttt tcatcgttaa
     1201 acagaaatcc ggccagcgct ttggcggttt cggtcttgcc cacgccggtg ggccccagga
     1261 acataaacga tcccagcggc cggctttcct cggcaatacc cgcgcgggcg cggcggatcg
     1321 cgttggcaat cgcgccaatc gccgcctcct gcccgatcac ccggcgcttt aaaatctctt
     1381 ccaaat
//
LOCUS       DAOQWE010000205         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_121102_length_1385_cov_4.011278, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000205 DAOQWE010000000
VERSION     DAOQWE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121102_length_1385_cov_4.011278"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PKK37_04760"
     CDS             <1..1283
                     /locus_tag="PKK37_04760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNR81720.1"
                     /translation="NAGLATHLGPSGKTCSEDPAGCMSYGLVGQWDMDEGSGSILNDK
                     SGNNNSCSLVNGPVWSKGKTGSALYFDGENDYADCNTAANDMSSVVGTVELWAKADRG
                     IGFPFHSNANIRTYLYRGASGFSVSKGDPMVSINFPNTPIGEWHHLVLTWDAGVFWGY
                     QDGVLIDSKSFSGTATATIVGIGRHASGGSSDYYDGDVDGVKIYNRALSEEEVRYHYN
                     QGKPVGYWAMDEGEGTRAFDVSGNNNTGVLTNGPTWVEGKHGSALSFDGVDDYVGCGT
                     DSSLNIADSMTASAWIYPKTLGEGSAGHIVGRGGTSGWCLYVYTGNSLKLYAGTIQAV
                     SSANSITLNQWQYATVVYDKQNVRFFVNGVEKGVTAATSSIVNTGSCVIGARTTAGDF
                     DFNGYLDEVKVYNYARTAEQIMQDYNAGVATHLK"
BASE COUNT          361 a          243 c          429 g          352 t
ORIGIN      
        1 ataatgccgg gctggcaacg catttggggc ccagcggcaa gacttgcagt gaggatccgg
       61 ccggctgtat gagctacggg ctggtggggc aatgggatat ggacgaaggg agcggatcaa
      121 ttctaaacga caaatcgggc aacaacaata gctgttcgct ggttaatggt ccggtatggt
      181 caaaaggaaa aaccggcagt gctttatatt ttgatggcga aaatgattac gccgattgca
      241 atacggcggc taatgatatg tcaagcgttg tcggaacggt ggagctttgg gcaaaagcgg
      301 acagaggtat tgggtttcca ttccattcca atgcgaatat aaggacatat ttatatagag
      361 gcgcttctgg attcagtgtg tcaaagggcg accctatggt atcaattaat tttccgaata
      421 ctccaatcgg agagtggcat catttggtgc tgacttggga tgccggcgtt ttttggggat
      481 accaagacgg cgttttgatt gattcaaaaa gtttttctgg aactgcaact gcaacaatag
      541 ttggaattgg gcggcatgcc agtggtggtt caagtgatta ctacgatggt gatgttgatg
      601 gggtgaaaat ctataaccgg gcgttgagcg aggaggaggt gcggtatcat tataatcagg
      661 ggaagccggt ggggtattgg gcgatggatg agggcgaggg gacgcgggcg tttgatgttt
      721 cgggcaacaa caacaccggg gttttgacca acggcccgac ttgggtggaa ggcaagcacg
      781 gcagtgcttt gagctttgac ggtgtggatg attatgtggg gtgcggcacg gattccagct
      841 tgaatatcgc cgattcaatg acggcaagcg cctggatata tccaaaaact ttgggcgaag
      901 gcagtgcggg ccatatcgtt ggccggggcg gtaccagcgg ttggtgtctt tatgtttata
      961 ccggcaatag cttaaaatta tatgccggga cgatccaagc ggtaagtagc gcaaattcaa
     1021 tcacattgaa tcaatggcag tatgcgaccg tggtctatga caagcagaat gttcgctttt
     1081 ttgtgaatgg ggttgaaaag ggtgtgacgg cggcaaccag tagcattgta aataccggaa
     1141 gttgtgtcat cggcgccagg acgacggcgg gtgattttga ttttaacgga taccttgacg
     1201 aggtgaaggt ttataactac gcgcggacgg cggagcagat aatgcaggac tacaacgccg
     1261 gagtggccac gcacttgaag tgataaattg ataattgata aatgataatt gataaataat
     1321 aattgataat tgataattag cggtttgctt ttttggggat cggctgtatt atgcaaatat
     1381 gatca
//
LOCUS       DAOQWE010000206         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_123261_length_1370_cov_7.787833, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000206 DAOQWE010000000
VERSION     DAOQWE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123261_length_1370_cov_7.787833"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1370
                     /locus_tag="PKK37_04765"
     CDS             <1..>1370
                     /locus_tag="PKK37_04765"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNR81721.1"
                     /translation="TVSGTGEVSPARETALKSKTAGKVLEIAVVAGQSVKAQDVIARI
                     DDDDAQDVVYDARVALESAQIALDELYEPADDLALTQAENALVQAKESLASDQDDLAQ
                     SYKQGFTSAASAFSSLSSIISGLQDIVAGASSYVTQSNPTYLDYYADAIKDYDSQASL
                     LTAQSAAAWRQAKDQYDRNFADYKKTSRESSNEEIENLLNQTYETAKLAQEALKNTAT
                     LVQRYKDVMENQNLTAQTFSETHLSSLSSYTTQNNSNLSNLLSAKQAIDNAESAVVNA
                     ERTLDEKTKSLDKLTQAPDESDIKSKTLAVEQKQRALDAAREALADCTIVAPFDGVVA
                     AIDIKAGDEIASGASVATIITANQVASITLNEVDIAKVEAGQKATLSFSAIDGLTITG
                     KVAQVGVMGSVSQGVVSYEVVIALDEANPQVKSGMSVSAAVITQIKQDVLLVPSTAVK
                     TANDGS"
BASE COUNT          364 a          315 c          394 g          297 t
ORIGIN      
        1 aacggtttcg ggcaccgggg aggtgtcgcc ggcgcgggaa acggcgttaa aatcaaaaac
       61 cgccgggaag gttttggaga tcgcggttgt tgccggccaa tccgtaaaag cgcaagacgt
      121 cattgcgcgg attgacgatg atgatgcgca agacgtcgtt tatgacgcgc gcgtggcgtt
      181 ggaaagcgcc caaatcgcgc tggatgaatt atatgagccg gctgatgact tggctcttac
      241 tcaggcggaa aacgcgctgg ttcaagccaa agaatctttg gcaagcgatc aagacgatct
      301 ggcgcaatca tataaacaag gattcacttc ggcggcgagc gcgttttcca gcttgtcgtc
      361 aattatttcc ggattgcagg atattgtcgc cggcgccagt tcctatgtaa cgcaatccaa
      421 tccgacttat cttgattatt atgctgatgc catcaaggat tatgattcac aggcgtcatt
      481 gctgacggcg cagagcgcgg cggcgtggcg gcaagcaaaa gaccagtatg accggaattt
      541 tgccgattat aaaaaaacga gccgggagtc ttcaaacgaa gagatagaaa atttgctcaa
      601 ccagacctat gagaccgcca agctggcgca agaagccttg aaaaacacgg ccactttggt
      661 ccagcgctac aaagacgtga tggagaatca aaatttgacg gcgcaaactt ttagcgaaac
      721 gcatttgtcg tccttgtcaa gctatacgac tcaaaataat tccaatttaa gcaatttgct
      781 ttccgccaaa caggcgattg acaacgccga aagcgcggtg gttaatgccg agcgcactct
      841 tgatgaaaaa accaaatcat tggataaatt gacgcaagcg cccgatgaat ccgacatcaa
      901 gtcaaaaaca ttggcggtgg agcaaaaaca acgggcgctt gacgcggcgc gggaagcgct
      961 ggcggattgc acgattgtcg ctccgtttga tggtgttgtc gctgcgattg atatcaaggc
     1021 gggagacgag attgcttcgg gagcgtccgt ggcgacgatt atcaccgcca atcaagttgc
     1081 ttcaatcacc cttaacgaag tggatatcgc caaggttgaa gccgggcaaa aagccacttt
     1141 atcgttttcg gcgattgacg gacttaccat caccggcaaa gtggcccaag tgggcgtgat
     1201 gggatcggtg agtcagggag tggtgagcta tgaagtggtt atcgctctgg acgaagctaa
     1261 tccgcaggtg aaatcgggga tgagcgtgtc ggcggcggtg attacgcaga ttaaacagga
     1321 tgtccttttg gtgccgtcaa cggcggtgaa aaccgccaat gacggttcgg
//
LOCUS       DAOQWE010000207         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_124156_length_1364_cov_7.170359, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000207 DAOQWE010000000
VERSION     DAOQWE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124156_length_1364_cov_7.170359"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKK37_04770"
     CDS             complement(<1..463)
                     /locus_tag="PKK37_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81722.1"
                     /translation="MPQTSDQNNNPIDLRELANLINDRFDKVDQTYRELAKTSNDRFD
                     KIDDKFDKIDDKFDKIDDKFAKIDDRFAQIDARFAQIDARFNKVDARFAKMDQANCEL
                     LEFLGEQFDKMNAKIDTKADKADTATKEDINLVLNRIAMINTKTDDYRAEQL"
     gene            complement(604..915)
                     /locus_tag="PKK37_04775"
     CDS             complement(604..915)
                     /locus_tag="PKK37_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81723.1"
                     /translation="MKLTISIGRNAFVFFCPYFVDKEDKYIETVRLGDGGEILVFFDE
                     RASHIKETLAPFSPSQVMYGLSSYSPEGLLPMKIELGMWRFEIVGTLTIIADSVSAEI
                     S"
     gene            complement(1203..>1364)
                     /locus_tag="PKK37_04780"
     CDS             complement(1203..>1364)
                     /locus_tag="PKK37_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HNR81724.1"
                     /translation="AVKEITQKGLKEAKDLVDASASAPQVIKEAVEKKEADELKAKLE
                     AAGAKVELK"
BASE COUNT          351 a          323 c          279 g          411 t
ORIGIN      
        1 gtaattgttc cgcgcgatag tcgtcggtct tggtatttat catcgcgatg cgattcaaaa
       61 caagattaat gtcttctttg gtcgcagtat cggctttgtc ggccttggtg tcaattttgg
      121 cgttcatctt gtcaaattgc tcgcccaaaa actcaagcaa ttcgcaattg gcctgatcca
      181 ttttggcgaa ccgggcgtca accttgttga accgggcgtc aatttgggcg aaccgggcgt
      241 caatctgggc aaacctatcg tctatcttgg caaacttgtc gtcaatcttg tcaaacttgt
      301 cgtcaatctt gtcaaacttg tcgtcaatct tgtcaaaccg gtcattggaa gtttttgcca
      361 gctcccgata ggtttgatca actttgtcaa accggtcatt gataagattc gccagttcgc
      421 gcaaatcaat cggattgttg ttttgatctg atgtctgcgg catttttttg agttaaacgc
      481 gattcaaaca cgatttgaat atgatttgaa tatgatttaa gtataaccca aacaaaatct
      541 ccccgcaaac ccatatctaa aaaggcattt tgagcgtttc ggtcaatgaa taaaaaaaag
      601 gcgctaactg atttccgcgg aaacgctgtc cgcaataatt gttagcgtcc cgacaatctc
      661 aaatctccac attcccaatt ctattttcat tggaagtaat ccctccggag aataggagct
      721 caagccatac ataacctggc ttggagaaaa gggcgcgagg gtttccttta tgtggctggc
      781 tctttcatca aaaaaaacca gtatctctcc cccatcacct aatcttacgg tttcaatata
      841 tttgtcctct ttatcaacaa aataaggaca gaaaaaaacg aatgcatttc tcccgatgct
      901 gattgttaat ttcattaaat caccggtatt attgaaacac gctgaattat ctttgtcaaa
      961 tcgccacggg ataactactc accgggttaa cgtatttttg tattttaagg gtctatccct
     1021 cgctaatgcg agggttagac cctggcaaag aattgtaggc acaaaattaa aggggggcta
     1081 accctcgcag taacgaggga tagtccccct ttaattttag atccggtgaa tggttacggc
     1141 gggcgtgggc aaacaaaaaa gggcgaaatg cccttttttg tgatggtgtc ccatcaaaag
     1201 tcctacttta actccacttt cgcgccggcc gcttccaact tggccttcaa ttcgtcggct
     1261 tctttctttt ccaccgcttc tttgatcacc tgcggcgcag acgcggaagc gtccaccaaa
     1321 tctttggctt ctttcaatcc cttttgggtg atttccttca ccgc
//
LOCUS       DAOQWE010000208         1364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_124161_length_1364_cov_5.396486, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000208 DAOQWE010000000
VERSION     DAOQWE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124161_length_1364_cov_5.396486"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..297
                     /locus_tag="PKK37_04785"
     CDS             115..297
                     /locus_tag="PKK37_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81725.1"
                     /translation="MLLGPIFVTGKPKAKLLVDQMVKMRALSDHLLGPALMNVFIPVN
                     FFGSEKNHALMRRNVA"
     gene            294..>1364
                     /locus_tag="PKK37_04790"
     CDS             294..>1364
                     /locus_tag="PKK37_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81726.1"
                     /translation="MIGGNATCVDANYNCSGAPSSSNVLAQQNQDLSFLTSAQNVADG
                     GACPCGLAHTGVCSAGAPSVNGGYCKNGNCVDGKCKLLECAISDSDGKVIARIGEGVK
                     TCTKPSVLSGDGVYVCGEDSQEPQKLYSSDGSLSSEKEPFSGTIVYNLNLEETCYSGC
                     SNGKCNDEAKCENLAKTIQDQFAKSYQPQTLACSNLSGDTQNEEYNKCKDCYDKVKTN
                     TNKALSGFGDIVKVFEKLAYPNGLSCGGVWVISPDPNKSITNSAISSIRTDDILCAKT
                     DTGAGGMTMNTACGKISVTGIQKNKICVGKDGEKFSSFLRGKSFDCKVINGCGVAGWI
                     LGDSPEKCNPGETNAFTLSDYFS"
BASE COUNT          383 a          266 c          360 g          355 t
ORIGIN      
        1 actttttggg aaacctttcg ggcggctcgg gcaaaataaa gatcgcgatc ttcgggcttt
       61 ttgttttaat ggtcgcggtt ttctttgctt tcagtggtat gcttgagcaa aattatgttg
      121 ctggggccaa tatttgtaac agggaaaccc aaggccaaac ttcttgtgga ccaaatggtg
      181 aaaatgagag cgctgtcaga tcatctgctt gggccagcac ttatgaatgt atttattccg
      241 gtgaattttt ttgggtcaga aaaaaatcat gcgctaatga ggagaaatgt tgcgtgatcg
      301 gcggtaatgc gacttgtgtt gatgcaaatt ataattgtag cggcgcgcct tcatcgtcca
      361 acgtactggc gcaacagaat caggacctct cgtttttgac cagcgcccaa aacgtggcgg
      421 atggcggtgc ctgtccttgc gggttggcgc acaccggagt ttgtagcgcg ggcgctcctt
      481 cagtcaatgg gggttattgt aagaatggaa attgcgtgga cggaaagtgc aagcttcttg
      541 aatgtgcgat tagcgatagc gacggcaagg taatcgcgcg gatcggggag ggagttaaaa
      601 cttgcaccaa gcccagcgtt ttgtcggggg atggcgttta tgtttgcggg gaagattctc
      661 aggaacccca aaaattatat tcaagcgacg gatcgttgtc ttccgaaaaa gaaccctttt
      721 ccggcacgat tgtttataac ttgaacctgg aggaaacatg ctattccgga tgcagtaatg
      781 gaaaatgcaa cgatgaggcc aagtgcgaaa atttggcgaa aacaattcaa gatcagtttg
      841 cgaaatcata tcaaccacaa accttggctt gttcaaattt atcgggcgat acccaaaatg
      901 aagaatacaa taaatgcaag gattgttacg ataaggtgaa aacgaatacc aataaggctc
      961 tttcgggatt tggcgatata gtcaaagttt ttgaaaaatt ggcatatccg aacgggttaa
     1021 gttgcggcgg tgtatgggtt attagccccg accccaacaa aagcattacc aattcggcaa
     1081 tttcatcaat tagaactgat gatattcttt gcgccaaaac cgacaccggc gccggcggaa
     1141 tgacgatgaa cacggcgtgc ggcaaaatca gcgtgaccgg aatacaaaaa aacaaaattt
     1201 gcgtcggcaa agacggggag aaattttctt cttttttacg cggcaaaagt tttgattgca
     1261 aagtgatcaa tggttgcggc gtcgctggct ggatattggg tgattcgccg gaaaaatgca
     1321 atccgggaga gacaaacgct ttcacattaa gcgattattt ttca
//
LOCUS       DAOQWE010000209         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_124304_length_1363_cov_6.328746, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000209 DAOQWE010000000
VERSION     DAOQWE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124304_length_1363_cov_6.328746"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..688
                     /locus_tag="PKK37_04795"
     CDS             185..688
                     /locus_tag="PKK37_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81727.1"
                     /translation="MPIHLNYQGTVCQKCGAEFIAHQKNCPCPACGQTTDDFCDFAAE
                     TIATMKDHKQLYGNYFPQSWFSGSIADTVQGIIFELFDNYEAENPEDFTAFVLTQLED
                     AVWEEGQEHLAIQVEGIALEVFNIYRQDPDFKGDALKAKPINPKLKDIQPKPFDAPID
                     IDSAAGF"
     gene            866..>1363
                     /locus_tag="PKK37_04800"
     CDS             866..>1363
                     /locus_tag="PKK37_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81728.1"
                     /translation="MQKAENVIKGKLAEGLVEQMLKASGCRVYRLGAEAALENIAQLE
                     DEFSKDSDLGKKMASIPDFAVMGCRERPVFVEVKFRAEPESLEEELLLEKDAQEGFWK
                     AKIVLVTTKTKPYFRVLTPPYFSKEKREGWPIPVANWKPLAQDEDLGCGEDIIKEFEE
                     LAEKYY"
BASE COUNT          396 a          267 c          361 g          339 t
ORIGIN      
        1 aagattggtg gatggcgatt cccgaaatcg cggccattgc cgaaaaaaat tcgggcaaga
       61 agtgttgtta aattttcgtg cgatagcata atcgataagg ttgcaacaat ataaatgcag
      121 ttttatggag gtcgcacccc tgctacgcta aagcttcgca ggggaacgac ccctcaaaaa
      181 ttcaatgccg attcatctta attatcaggg gacggtttgt caaaagtgcg gcgcggaatt
      241 catcgcgcat cagaaaaatt gcccatgtcc ggcctgcggc caaaccacgg atgatttttg
      301 cgattttgcc gccgaaacga tcgcgacgat gaaagaccac aaacagcttt acgggaatta
      361 ttttccgcaa agctggtttt ccggttcaat cgcggatacc gtgcagggaa taatttttga
      421 attgtttgat aattatgagg ccgaaaatcc cgaagatttc accgcgtttg ttctgacgca
      481 attggaggac gcggtctggg aagaagggca ggaacattta gccatccaag ttgaagggat
      541 cgcgctggaa gtttttaata tctaccgcca agatccggat ttcaaaggcg atgctttaaa
      601 agcaaagccg ataaatccaa aattgaaaga tattcagcca aaaccgttcg atgcaccgat
      661 tgatattgac agcgcggcgg gtttttaaaa attggcatcc ttcatttttt gccttgaaag
      721 aggggctttt tttgtataat atcaaggata agttaagatt acaaaatgca gtttaccgaa
      781 gtcatatctt ggcgattaag ggaagatttt tttggaaggg tccgaccctt ttgattacaa
      841 aagggtcgga cccttgaaat tcaaaatgca aaaagcggaa aatgttatta agggcaagct
      901 ggccgagggg ctggtggagc agatgcttaa ggcaagtggt tgccgggttt atcgtttggg
      961 tgccgaagcg gcgctggaga acatcgctca attggaagac gagttttcca aggacagcga
     1021 tctgggcaaa aagatggcgt caattcccga ttttgccgtg atgggatgcc gcgagaggcc
     1081 ggtgtttgtg gaagttaaat tccgcgccga acccgaatcg ctggaagaag aactgctcct
     1141 ggaaaaagac gcgcaagaag gtttttggaa agcgaagatt gttttggtga ccaccaagac
     1201 aaagccttat tttagagtgc tgacgccgcc ttatttttcc aaagaaaaac gcgaaggatg
     1261 gcccattccg gtggccaact ggaaaccgct cgcccaagac gaggatttgg gatgcggcga
     1321 agatatcatt aaagaatttg aagaattggc ggagaaatat tat
//
LOCUS       DAOQWE010000210         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_125718_length_1354_cov_3.304850, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000210 DAOQWE010000000
VERSION     DAOQWE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125718_length_1354_cov_3.304850"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..668
                     /locus_tag="PKK37_04805"
     CDS             57..668
                     /locus_tag="PKK37_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNR81729.1"
                     /translation="MENIVYVLVNEAMPGYVKIGKTNNLEQRIRSLDTTGVPLPFECF
                     YACAVKDADFVERQILDAFMDHRVRSSREFFEIAPERAVAILKLVEIKNVTPGSDFVE
                     SREDQQAINRAKERRAVFNFKMVDIPAGAELSFSRDENIKSKVIDSRNVEFGGEIMSL
                     SAAADKALRQIGIIWKSVQGPAYWIYDGELLDERRRRYEEAEE"
     gene            678..>1354
                     /locus_tag="PKK37_04810"
     CDS             678..>1354
                     /locus_tag="PKK37_04810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81730.1"
                     /translation="MPSLNWIGKDKITNHDKDVPFRLMKKNQKLSLGKSENLILEGDN
                     LEALKALMPFYYNKVKCVYIDPPYNTGNEKWIYNDKVNSPKIKEWLGKVVGPEGDDLT
                     RHDKWLCMMYPRLKLLRDLLSDDGFIFVSIDDIELTNLRILMNEIFGEENFVGLITIK
                     STPGGRQSNKNLAIMGDYILLYSKTNNAQILGLPLSKKQLKQYKKTDRSGQYLTERLR
                     QHGVAGK"
BASE COUNT          431 a          217 c          335 g          371 t
ORIGIN      
        1 gcttttggtg atttttgaaa gttgaaaaca aggggtgaaa gtgctaaaat catcatatgg
       61 aaaatattgt ttatgttttg gtcaatgagg caatgccggg ctatgttaaa atcggcaaaa
      121 cgaataattt ggaacaaagg attcgttcgc tggacaccac gggcgtgccg ttgccgtttg
      181 aatgttttta tgcttgcgcg gtgaaagacg cggactttgt ggagcggcaa attttggatg
      241 cgtttatgga tcatcgcgtt cgttccagtc gtgaattttt tgaaattgcc cccgaaagag
      301 cggtggcgat tttgaaattg gttgaaatca aaaatgtcac tcccggaagt gattttgttg
      361 aatcgcgcga agaccaacag gcgataaatc gcgccaagga aagaagagcg gtttttaatt
      421 ttaaaatggt ggatattccc gcgggcgcgg aattatcttt cagccgcgac gaaaacataa
      481 aatcaaaagt tattgacagt cgcaatgttg aatttggcgg agaaattatg agcttgtcgg
      541 cggcggctga caaggcgttg cgccaaatcg gaatcatttg gaagtcggtg caagggccgg
      601 cctattggat ttatgacggc gaattgcttg atgagcgccg ccgccgctat gaagaagccg
      661 aagaataatt ttttaatatg ccgtcattaa attggatcgg aaaagacaaa ataacgaatc
      721 acgacaagga tgtgccgttt cgcttgatga aaaaaaatca aaaattatcg ctgggaaaaa
      781 gcgaaaattt gattttggag ggcgataatc tggaggcgtt aaaagccttg atgccttttt
      841 attacaacaa agtgaaatgc gtttatattg atccgcccta taacaccggc aacgaaaaat
      901 ggatttataa cgataaagtc aactctccca aaattaaaga atggctggga aaagtggttg
      961 gtcccgaagg cgacgatttg acgcgccacg acaagtggtt gtgtatgatg tatccgcgct
     1021 tgaaactttt gcgcgacctg ctgtctgatg acggatttat ttttgtcagt atagatgata
     1081 ttgaactgac aaatttaaga attttaatga atgaaatttt tggagaagaa aatttcgttg
     1141 gtttgattac gattaaatca actcctggcg gtagacaatc aaataaaaat cttgcaatta
     1201 tgggggatta tattcttttg tatagcaaaa caaataatgc ccaaatttta ggtttgccgt
     1261 taagcaaaaa acaattaaaa caatataaaa aaacagatag aagcgggcag tatttaaccg
     1321 aaagattgag gcaacatgga gttgcgggga aaag
//
LOCUS       DAOQWE010000211         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_127476_length_1343_cov_4.673913, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000211 DAOQWE010000000
VERSION     DAOQWE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127476_length_1343_cov_4.673913"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1161
                     /gene="nagZ"
                     /locus_tag="PKK37_04815"
     CDS             121..1161
                     /gene="nagZ"
                     /locus_tag="PKK37_04815"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNR81731.1"
                     /translation="MIRPLKEKIGQMLMVGFRGTEIDDDSEIAKTIEELNLGGVILFD
                     YDAPSKSRPRNIVDREQTKKLVADLKGYAKNEPLLVAVDAEGGLINRLKPSMGFSKIS
                     SAQSLAEKSGLAAINEYWTLARQLADLGFNINFAPVVDLNLNPESPAIGALERSYSDN
                     PETVAGYARTLIAAHRAFGVLTALKHFPGHGSADADSHLGLPDITASYEEQELTPYAE
                     LIGSGGADSVMTAHVINRNIDPEYPATLSEKFIKEILRQNLGFDGVVFSDDMQMGAIV
                     DNYGFEDAIVRAINAGCDVLILSNNGAEYDDSVARRARDIILQAVLDGQISESRINES
                     YARIIKLKEKLK"
BASE COUNT          362 a          334 c          347 g          300 t
ORIGIN      
        1 tggcggcggc ggaaaaaaat caacaaaagc gcgacagtga aagcgctggc ggtttttctg
       61 ttttgttcaa cgctggtttt tgaatatttc cacgccgaaa aaattgcccg ggaagaagaa
      121 gtgatccggc cgttgaaaga aaaaatcggc caaatgctga tggtcggctt tcgcggcacc
      181 gagattgatg atgattccga aattgccaag acaatagaag aattgaattt gggcggcgtg
      241 attttgtttg actacgatgc gccgtcaaaa agccggccgc gcaacattgt tgaccgggaa
      301 caaacaaaaa aactggttgc cgatctgaaa ggatatgcca agaacgaacc cctgctggtt
      361 gccgttgacg ccgaaggcgg actgatcaac cggttaaaac cttcaatggg attctccaaa
      421 attagctcgg cgcaatcctt ggcggaaaaa agcggcctgg ccgcgattaa cgaatattgg
      481 acgctggcgc ggcagttggc cgatttggga ttcaacatca atttcgcgcc ggtggtggac
      541 ctgaatctga atcccgaaag cccggcaatc ggcgcgctgg aaagatccta ttcggacaac
      601 cccgaaaccg tcgccggcta cgcccgcacc ctcatcgccg cgcaccgcgc cttcggcgtt
      661 ttgacggcac tcaaacattt tcccggccac ggcagtgcgg acgccgacag ccatttggga
      721 ctgcccgata tcaccgcctc ctatgaagag caggagttga cgccttacgc ggaattgatt
      781 ggaagcggcg gtgcggattc ggtgatgacc gcgcatgtga tcaaccgaaa cattgatccg
      841 gaatatcccg ccacgctttc ggaaaagttc atcaaagaaa ttctgcgcca aaatttggga
      901 tttgacggcg tcgtgttctc cgatgatatg caaatgggcg cgattgttga caattacgga
      961 tttgaggacg ccatcgtccg cgcgatcaat gccggttgcg atgtgctgat tctttccaac
     1021 aacggcgcgg aatacgacga ctccgtcgcc cggcgcgccc gcgatattat tctgcaggcg
     1081 gttcttgacg gccaaatctc cgaatcccgc atcaacgaat cttacgcccg cataataaaa
     1141 ctgaaagaaa agctgaaata aactacgccg gggcaaaacc ccaaggtatt ctggcgggta
     1201 aataaaatac cccggacaga acccgaagta taaagaacat ccttacatac tcaagtatgt
     1261 aaggatgttc ttttatgcaa attgaagcta gaattttaaa agttggcaag taatttgttt
     1321 tttgacgaca tcaatttttt ctt
//
LOCUS       DAOQWE010000212         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_128440_length_1337_cov_4.028861, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000212 DAOQWE010000000
VERSION     DAOQWE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128440_length_1337_cov_4.028861"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            498..>1337
                     /locus_tag="PKK37_04820"
     CDS             498..>1337
                     /locus_tag="PKK37_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81732.1"
                     /translation="MIEKVPFTRENEERKRKEERYKRARAEYVRKTWAYNSAAANSLK
                     LYIDKAAIAEIARKKQAELEKTLRQIEREKDAQKAQADRLRALNLVDVLKTIYSAKEE
                     KDSKPEYSSRKFVLPDGRKIGVTGNLWVYNDGSNRGGKNAINLVMSLDNLDYKGAVRL
                     LADHFEYGVVASDLARSAVEKIEKEVETIAKSEPVPVPIPAESKWGIVKKYLCEIRGL
                     PSKLIDQLHSIGLVFADSNENAVFRRVKGGAFVRGTKGDFKRTIGKADCGPFLIPGKP
                     EKGI"
BASE COUNT          429 a          238 c          343 g          327 t
ORIGIN      
        1 atattgtaga ggcggcaaaa aaggttatcg gggatcaaaa aatacgtaaa aacgcagttt
       61 tgggggtgga agtgctgtta acagcgagtc ccgaatattt tcgccccgaa tgtcccaaaa
      121 aagctggata ttatgacgag gagcggctta aggcgtttcg tgatgttgcc gaaaagttca
      181 tttctgagca ttttccacat gctgttagcg tagttttgca ccttgatgaa tctacgccac
      241 attaccacat aatcgatatt ccgcttgatg aaaaaggcaa gttgaacgcg cgcgaaaaat
      301 acggcggcgc aaaaaaatta tcggaatggc aggataaggt tgccgcggct tttgaaaatg
      361 tggggttgga tcgcggcata aagaaaagca aagcaaaaca tgtaaaaatc aaggaatttt
      421 acgggaatat taacagcaat ttgccggatt taccggaacc ggcgccgaat ccgccgaaac
      481 cgttgccgcc gaaaaaattg attgaaaaag ttccgtttac acgcgaaaat gaagagcgga
      541 agcggaaaga ggaacgatat aaacgtgcgc gcgctgagta tgttcgcaaa acttgggcat
      601 ataattcagc tgcggcaaat agtttaaaac tgtacattga caaggcggca attgccgaga
      661 ttgcgcgaaa gaaacaagcg gaattggaaa aaacattgcg gcaaattgaa cgcgaaaaag
      721 acgcgcaaaa agcgcaagcg gatcgtttgc gcgcattaaa tcttgttgat gttttgaaaa
      781 cgatttacag tgcaaaagaa gaaaaagata gtaaaccgga atattcttcg cgcaaatttg
      841 ttttgcctga tggtagaaaa atcggtgtta ctggtaattt gtgggtttat aatgacggtt
      901 caaatcgtgg tggaaaaaac gcaattaatc tagtaatgtc tcttgacaat cttgattaca
      961 aaggggcggt acggctcctg gcggatcact tcgaatacgg cgtggttgcg tccgatcttg
     1021 cgagatcggc tgtcgaaaaa attgaaaaag aggtggaaac aatcgcaaaa tctgaacctg
     1081 ttcctgtgcc gatacctgcc gaatcaaaat ggggaattgt gaaaaaatat ctttgtgaga
     1141 ttcggggctt gccatcaaaa cttatcgatc aactgcattc gattggtctt gtttttgctg
     1201 atagcaatga aaatgcggtt ttccgtaggg taaaaggtgg cgcgtttgtt cgtggtacaa
     1261 aaggcgattt taagcgaacg atcggcaaag ctgattgtgg accattctta attcccggaa
     1321 aaccggaaaa aggaatt
//
LOCUS       DAOQWE010000213         1335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_128755_length_1335_cov_9.038281, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000213 DAOQWE010000000
VERSION     DAOQWE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128755_length_1335_cov_9.038281"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..517)
                     /locus_tag="PKK37_04825"
     CDS             complement(218..517)
                     /locus_tag="PKK37_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNR81733.1"
                     /translation="MEAKLENKKIKTPKQFERYFKGAANHRRIEIIFIISRQQGITLD
                     GITEILGGSFKTISEHTKKLVQSGLVNKKYCGRQVQHELSPYGMKFLEFMKAFSD"
     gene            complement(633..1271)
                     /locus_tag="PKK37_04830"
     CDS             complement(633..1271)
                     /locus_tag="PKK37_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNR81734.1"
                     /translation="MSVNICVFGDSIAFGKYDPCGGWAARLAEFLEARCLAKKGDEFF
                     VYNLSISGNHTGDILARFETELKPRFSEKREWVVVFAVGLNDSAVVHSKNDNWVGFGE
                     FQENIKKLIGLADGFSDKIVFVGPTSPDYQIVDPMPWDLDKSYREAEVKKYDAALRDI
                     CQKYEVGYIPLCEKFIANDHKKLLHDGAHPNSAGHQLIFETVRDHLLEQKII"
BASE COUNT          390 a          330 c          222 g          393 t
ORIGIN      
        1 tttgccgcca acagcgccgc cggcaccagc cctccccggc taacgccgac cacaaattca
       61 accttatcca aaaaatcttt gttatcgccg gcaaacttac cgattatttt ttcaatctcc
      121 caaaacccaa cttccacctt tcccatatta ttagcagaca ttccgacatt ctgcagaatg
      181 tcggaatatc agtaattgaa ttattaacga catggcttta atcagaaaac gccttcataa
      241 attctaaaaa tttcattcca tatggagata actcatgctg tacttgccgg ccacaatatt
      301 ttttatttac caatccagat tgcaccagct tttttgtatg ctcggaaatt gttttaaaac
      361 tgccacctaa aatttcagta attccatcaa gcgttattcc ctgttgtcgc gatattataa
      421 aaattatttc gatccggcga tgatttgcgg cccccttgaa atatcgttca aattgcttgg
      481 gcgttttgat tttcttattt tctaatttcg cttccatgga ttgcaaatta ttaaaaattg
      541 atacccctca aaataattat ataacaacca caaaaacatt ccaacattct gcagaatgtt
      601 ggaatgttgt aagcacaaaa catagaagga tactaaatga ttttttgttc cagcagatgg
      661 tcgcggacgg tctcaaagat cagttgatgg ccggcggaat tgggatgcgc gccgtcgtgc
      721 agtaattttt tatgatcgtt ggcaataaat ttttcgcaaa gtggaatata accaacttcg
      781 tatttttggc aaatatcgcg caaggcggca tcatattttt tgacctctgc ctcgcgataa
      841 gatttgtcca aatcccaggg catcgggtca acaatttgat aatccggaga agtcggaccg
      901 acaaaaacaa ttttatcgga aaacccgtcc gccaaaccaa tcaatttttt aatattttct
      961 tgaaattcac cgaacccgac ccagttatca tttttgctat gaacaactgc ggaatcattt
     1021 aatccaacag caaaaacgac aacccattct cttttttcgg aaaaccgcgg ctttagttcg
     1081 gtttcaaaac gcgccagaat atcgcccgtg tgattgccgg aaatcgacag attatacaca
     1141 aaaaattcat cgcccttttt ggccaaacat ctcgcttcca aaaattcagc caaccgcgcc
     1201 gcccagcccc cgcacggatc gtatttccca aacgcgatgc tgtcgccgaa aacgcaaata
     1261 ttgacgctca taatcaaagt tttattttct ttttctcggc gtcaactctt tccgcgactt
     1321 ttcgcaccag cgacg
//
LOCUS       DAOQWE010000214         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_129974_length_1328_cov_4.428123, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000214 DAOQWE010000000
VERSION     DAOQWE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129974_length_1328_cov_4.428123"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKK37_04835"
     CDS             complement(<1..748)
                     /locus_tag="PKK37_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR81735.1"
                     /translation="MTLETKNLACGYNGAIAAENISFRIESGQTLCLLGPNGAGKTTL
                     LKTILGFIKPKRGDILIDGKNINCLSAKKISQSIAYVPQIHTPPFPFSVLDVVTMGRN
                     VHLGMFASPSKKDYALAEETLASLGVSFLRDRIYTEISGGERQMVLLARALTQQPQIL
                     AMDEPTLNLDFGNQTRVLNQIRRLAQRGIGIIMISHFPDHAFLLSGKAALMRKNGPFI
                     FGNTDEVITEGNLKSAYGINVKITGINMDGG"
     gene            complement(790..>1328)
                     /locus_tag="PKK37_04840"
     CDS             complement(790..>1328)
                     /locus_tag="PKK37_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR81736.1"
                     /translation="SSLFSSLISIAKYAADTDDKLPAITFWLMGSLSSINAGDLKVMI
                     IPLAAGAALIFLLRWKLNIISFGDEEARNMGVDVKKIRPLIIAAASLLTATAVSAGGM
                     IGWVGLIIPHVARFLVGPNYKILLPVSFLCGSLFLLAIDNIARTAFTVEIPLGILTSL
                     IGAPFFVYLMFRGRKSWV"
BASE COUNT          331 a          321 c          357 g          319 t
ORIGIN      
        1 cgccgccatc catattgatg ccggttattt tcacattgat tccgtaagcc gatttcagat
       61 tgccttcggt aatcacttcg tcggtgtttc cgaaaatgaa cggcccgttt tttcgcatca
      121 gcgcggcttt tcccgagagc aagaacgcat ggtcgggaaa gtgtgaaatc ataatgatac
      181 cgattccgcg ttgcgccaat cgccggattt gattgagcac gcgggtttgg ttgccaaaat
      241 caagattcag cgtcggttcg tccattgcca gaatttgggg ctgttgcgtc agcgcccggg
      301 cgagcaaaac catttgcctt tccccgccgc tgatttcggt atatatcctg tccctcaaaa
      361 atgatactcc cagagacgcc aaggtttctt cggccagagc gtaatctttt tttgacgggg
      421 aagcaaacat tcccagatgg acattgcgtc ccatcgttac cacatccaga accgaaaacg
      481 gaaacggcgg agtgtgtatt tgcggaacat aggcgatgct ttgggaaatt ttttttgcgg
      541 acaggcaatt tatatttttt ccgtcaatca aaatatcgcc ccgtttcggc ttaatgaagc
      601 ccagaatcgt tttcagcaaa gtggttttgc cggcgccgtt gggtcccaga agacagaggg
      661 tttggccgga ttcaatccga aagctgatat tttcggcggc gatggcgccg ttatagccgc
      721 aagcgagatt tttggtttca agcgtcatgg ttttgttgca aaacggtaaa gcgattgtct
      781 gcggcgatgt tatacccacg attttcttcc ccggaacatg agataaacaa agaagggagc
      841 gccgatcagc gaagtgagga ttcccagggg aatttcaacc gtgaaggcgg tgcgcgcgat
      901 attgtcgatt gccaacagaa acaaactccc gcagagaaag gaaacgggca aaaggatttt
      961 gtaattcggg cccaccagaa aacgggcgac atgaggaatt atcaatccca cccagccgat
     1021 catcccgccc gcggaaacgg cggtggcggt gagaaggctg gcggcggcga tgatcagcgg
     1081 ccggattttt ttaacatcca ctcccatatt gcgcgcttct tcgtcgccga aggatattat
     1141 gttcaacttc cagcgcaaaa gaaagatgag ggccgcgccc gccgccaggg ggattatcat
     1201 cactttcaag tcgccggcgt tgattgagga aagacttccc atgagccaaa aggtgatcgc
     1261 cggcaatttg tcatcggtgt cggcggcgta tttggcaatg gaaatcagcg aagagaaaag
     1321 cgaagaaa
//
LOCUS       DAOQWE010000215         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_132156_length_1316_cov_1.978588, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000215 DAOQWE010000000
VERSION     DAOQWE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132156_length_1316_cov_1.978588"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..838)
                     /locus_tag="PKK37_04845"
     CDS             complement(32..838)
                     /locus_tag="PKK37_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HNR81737.1"
                     /translation="MTHEKIKEAFDYFYASNNIAYDARKHQYKLDGITCAGVSTIAEY
                     RPAPFLKFWAAKMVIEHLADKQEIIKNLSPKEYQELLLEAKKAHIRKSDTALEIGTAA
                     HDWADKHIHGEDLPITKEIENCAQAFLDFEANHQTQWICTEKIVVNREHLVAGRLDSL
                     AFVDGALSLVDLKTSSRVSESYYLQTAGYALCLQDMGIPVERRIILRLPKVKDEEFEA
                     VEVDTPLAADTKAFLAQRYAYEWANMIDCKHTETINRGKFKEKKLKLKKL"
     gene            complement(835..1197)
                     /locus_tag="PKK37_04850"
     CDS             complement(835..1197)
                     /locus_tag="PKK37_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81738.1"
                     /translation="MNQFYDEKDINQQIAENQYYDIRDTISKVRNAQAPLKLDGIAAA
                     IADEIDDVQELTKLVAELDWHINQKINRDAVVFGNATKKANEGNSVAMGGIPKDNTAL
                     LKDMNEVFFGGVNKALGV"
BASE COUNT          286 a          329 c          271 g          430 t
ORIGIN      
        1 tggtattgat taaatttcat agtttaattt gttatagttt tttcaatttc aatttttttt
       61 ctttgaattt cccgcgattg attgtttcgg tatgcttgca atcaatcata ttcgcccact
      121 catacgcata ccgctgggca agaaacgcct ttgtgtcggc cgccaaaggc gtgtcaactt
      181 ctaccgcttc aaactcctcg tcttttactt tgggcaaacg caagataatt cttcgctcaa
      241 cggggatacc catatcttgc aggcacagcg catatccggc ggtttgcaga taatagcttt
      301 cactaacccg actgctggtc ttcaaatcta ccagcgataa cgcgccgtcc acaaacgcca
      361 agctgtccaa tctgcccgcc accaaatgct cccgattgac aactattttt tcggtgcaaa
      421 tccactgcgt ctgatgattt gcttcaaagt ccaaaaatgc ttgggcgcaa ttttcaatct
      481 ctttggtaat gggcaaatcc tcgccgtgaa tatgtttgtc tgcccaatcg tgcgccgccg
      541 tgccaatctc caatgccgtg tcgcttttgc gaatatgcgc ttttttggct tccagcaaaa
      601 gctcttgata ttcttttggc gaaagatttt tgattatttc ctgtttatcc gccaaatgct
      661 ctatcaccat ttttgccgcc cagaatttga ggaaaggcgc ggggcgatat tcagcaatcg
      721 ttgatacacc cgcgcaagtt atcccatcca gcttgtattg gtgcttgcgg gcgtcatagg
      781 caatgttatt gctggcgtaa aaataatcaa acgcttcttt aattttttcg tgtgtcatac
      841 gcccaacgct ttatttactc cgccgaagaa aacctcgttc atatctttga gcagggcagt
      901 gttgtctttg ggaatgcccc ccattgccac agaattgccc tcatttgcct ttttcgtggc
      961 gtttccgaac acaacagcgt ctcggtttat tttttggtta atatgccaat ccagctcggc
     1021 gacaagtttg gtgagttctt gcacatcgtc aatctcatcg gcgatggcgg cggcgatgcc
     1081 gtccaacttc aaaggtgctt gcgcgttgcg gactttgctg attgtgtcgc ggatgtcgta
     1141 gtattggttt tccgcgattt gctggttgat gtctttttca tcgtaaaatt ggttcatatt
     1201 tttttagtta ttgattaaat cttcaatgtc aattccattt tttctgtctt ctccagtaat
     1261 ttggcactca tcaaggtcgc cccttacccc cgtaaggtcg ccacgcaccc cagaga
//
LOCUS       DAOQWE010000216         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_132202_length_1315_cov_5.958730, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000216 DAOQWE010000000
VERSION     DAOQWE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132202_length_1315_cov_5.958730"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..>1315)
                     /locus_tag="PKK37_04855"
     CDS             complement(45..>1315)
                     /locus_tag="PKK37_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR81739.1"
                     /translation="ERDVWYERITAGESDECAPALMSAEDPLFILYTSGSTGKPKGAL
                     HTCGGYMVSAYASDKWIFDLKDDDIFWSTADIGWVTGHTYSCYGPLLNGATFVIFEGL
                     ADYPDPGRWGQMIDKYGVTVFYTAPTAIRMFIKNGEEAITPYHFKTLRILGSVGEPIS
                     EKAWLWYFVHAGKKRCPIVDTWWQTETGGIVATSLPGIGPFKPAHTGLPLPGIRLRVL
                     DEKGKLCKPGEEGNVCLLAPFAPGLLRGVWGDKRKFLETYWSEYPRKTIYFSGDLGVY
                     DKDGMLRVVGRADDQIKVAGHRLTTGEMESAACTEAAVAECAVVGIADDIKGEVPAAF
                     VVPKPEKDISELKEKVIAAIRRQIGPIAAPHKVFIVPDLPKTRSGKILRRLLRKIIAG
                     ENPGDLSTLANPESVESIKAAVFVENAEKK"
BASE COUNT          286 a          393 c          325 g          311 t
ORIGIN      
        1 atgtttggtg atatatatat tcaactggat attatgtcac tttattattt tttttcggcg
       61 ttctcgacga ataccgcggc tttgatgctt tccacgcttt cgggattggc caaagtggaa
      121 aggtctcccg ggttttcgcc ggcgataatt ttgcgcaaca gacggcggag gatcttcccc
      181 gaacgggttt tgggcaaatc cggcacgata aaaactttgt gcggcgcggc aatcggcccg
      241 atctgccttc tgatcgcggc gataactttt tccttcaatt ccgaaatgtc cttctccggc
      301 ttgggaacca cgaacgccgc cgggacttcg cctttaatat cgtcggcgat gcccaccacc
      361 gcacactcgg ccaccgccgc ttcggtgcaa gcggcgctct ccatttctcc ggtcgtcaaa
      421 cgatgtccgg cgactttgat ctgatcatcc gcccggccga ccacccgcaa cattccgtct
      481 ttgtcgtaga cacccaaatc accggaaaaa taaatcgttt tccgcggata ttcgctccaa
      541 taggtttcca aaaattttcg tttgtcgccc cacactccgc gcaacagccc gggagcgaac
      601 ggcgccagca agcaaacatt gccctcctcg ccgggtttgc ataacttccc tttttcatcc
      661 agaacccgca ggcggatgcc gggcagaggc aagccggtgt gcgccggctt gaaagggccg
      721 atgccgggca atgacgtggc cacaattccg ccggtttcgg tttgccacca agtgtccacg
      781 atcgggcatc ttttctttcc ggcgtggaca aaataccata accacgcttt ttccgagatc
      841 ggctcgccca ccgaacccag aatccgcaaa gtcttgaaat gatacggagt aatcgcttcc
      901 tcgccgtttt tgatgaacat cctgatcgcc gtcggcgcgg tgtaaaaaac cgtcacgccg
      961 tatttgtcaa tcatttgccc ccaccggccg gggtcgggat aatcggccag tccttcaaat
     1021 atcacgaatg tcgcgccgtt taacaacggc ccgtagcagg aataagtgtg gccggttacc
     1081 cacccaatat cggcggtgga ccaaaaaata tcatcgtctt ttaaatcaaa aatccatttg
     1141 tcggaagcgt aggccgaaac catatatccg ccgcaggtgt gcagggcgcc tttgggcttg
     1201 ccggtggagc cgctggtata aagaatgaac agcgggtctt cggcagacat taacgccggc
     1261 gcgcactcgt cggattcacc cgccgtgatc cgctcatacc aaacatcccg ttcgc
//
LOCUS       DAOQWE010000217         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_133566_length_1308_cov_1.909018, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000217 DAOQWE010000000
VERSION     DAOQWE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133566_length_1308_cov_1.909018"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PKK37_04860"
     CDS             complement(<1..668)
                     /locus_tag="PKK37_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010474104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81740.1"
                     /translation="MKKSQIFYGNSHREEIEQKLKKFLNEQADFLSARTINSPRAVGD
                     AIENVIAENFKAILGDLVGEYSSSFARRAMADLAFTDKDGFYYVVDVKTHRLDTKFNM
                     PNLTSVERLSRFYEEDINYFSLLKIDYKINGTRAEIDKVTFAPIEFFDWDCLTIGALG
                     WGQIQIANANVVKIVPKNSRKKWMLELCDTMLEFYPKEIGKIGERIDHFKKINDTYGH
                     QTGD"
     gene            complement(665..>1308)
                     /locus_tag="PKK37_04865"
     CDS             complement(665..>1308)
                     /locus_tag="PKK37_04865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNR81741.1"
                     /translation="FNVAKKYFKIQNRITFEREKGRGAKSNWKNCSEDIWFCTLSDKY
                     HFDVDAVKMKRKVIAPYKDENGKPKDWEDGDNGKYRITHPSNVWTDITIPFWSMPENT
                     DHPTQKPEKLVAKVILASSKPNDVVFDPFLGSGTTSVVAKKLGRRYVGIEIDEHYCCL
                     AEKRLDITGNEKNIQGYSDGIFWERNTLNEQMNGNGKKNKIELSENLFSKKLI"
BASE COUNT          345 a          291 c          221 g          451 t
ORIGIN      
        1 tcatcgcctg tttgatggcc gtaggtatca ttgatcttct tgaaatgatc aatacgctcg
       61 ccaattttac caatttcttt aggataaaac tcaagcattg tgtcgcataa ttccaacatc
      121 cactttttcc tactattctt tggcactatc ttcaccacat ttgcgtttgc gatttgaatt
      181 tgaccccaac caagcgcgcc aatagttagg caatcccagt caaaaaattc aatcggagca
      241 aaagttacct tgtcaatctc tgcccgcgtg ccgttgattt tataatcaat ctttaggagc
      301 gaaaaataat ttatatcctc ctcgtaaaat cgcgacaacc tttcaaccga tgttaagttt
      361 ggcatattga attttgtatc gagccggtgc gtttttacat caacgacata ataaaatccg
      421 tctttgtcgg taaaagccaa atctgccatg gctcggcgtg caaaagatga cgaatactct
      481 ccaacaagat cgcccagaat ggctttaaag ttttcggcga taacattttc aatagcgtcg
      541 ccaaccgcgc gcgggctatt tattgttcgt gcggaaagaa agtcagcttg ctcattcaaa
      601 aactttttga gcttttgctc aatttcttct ctgtgcgaat tgccgtaaaa aatttgactc
      661 tttttcatat aagtttttta ctaaataaat tttctgatag ttcgatttta tttttctttc
      721 cgtttccgtt catttgctcg ttgagtgtat tgcgctccca gaatatgccg tccgagtatc
      781 cttgtatatt tttttcgttt cctgttatat ccaatcgctt ctcagcaaga caacaataat
      841 gctcatcaat ttctatacca acataccgac gaccaagttt ttttgcgaca accgaagtcg
      901 tacccgatcc aagaaacgga tcaaaaacaa catcatttgg ctttgaactt gccaaaataa
      961 ctttggcgac caatttttct ggtttttgtg ttgggtggtc ggtattctcc ggcattgacc
     1021 aaaacggaat tgtaatgtcg gtccaaacat tcgacgggtg ggtaattcta tatttcccgt
     1081 tgtcgccgtc ttcccaatct tttggctttc cgttttcatc tttgtatggt gctataactt
     1141 tacgcttcat ttttacagcg tcaacatcaa aatgatattt atcggaaagc gtgcaaaacc
     1201 aaatatcctc ggagcaattt ttccaattgc tttttgcacc gcgtcctttt tctcgttcaa
     1261 aagttattcg gttttgtatc ttaaaatact tttttgctac attaaaaa
//
LOCUS       DAOQWE010000218         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_136466_length_1292_cov_1.541633, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000218 DAOQWE010000000
VERSION     DAOQWE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136466_length_1292_cov_1.541633"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKK37_04870"
     CDS             complement(<1..424)
                     /locus_tag="PKK37_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81742.1"
                     /translation="MRPVVSAISSFDGVFVSPEFCRLAEFMAGTVSLGTDLFLSASVL
                     PGGVFMGGASNSFSFSARPLFSAGATFSFGRTFSARKTISGADFFSSETAIGLSASFL
                     AVPLSGAALSVCGRAGFAANLFSLSAFSSGALFSPFIFF"
     gene            446..>1292
                     /locus_tag="PKK37_04875"
     CDS             446..>1292
                     /locus_tag="PKK37_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81743.1"
                     /translation="MEAAPARNIRVFAQENSPKIVPTAEAEREMPNLVKNEPVRPNPS
                     ENEPETDRGNLAPNPENRWRQIKKTTLPGAAAFAPPDVKAEESRAQMATERPAAKSKR
                     NNLLARLLIIFAVIAVLGIILAVILIKNGGKTPAVVKSEPKPTMTTEKNLPEEPAAPA
                     PIVTTLTTVTIYVDSPANIPSSISPYMRKKFDANGYHSLLIVDRGTGKKIGLKQFFSA
                     YKLNAPSIFYNSIEDDCEFFIYANNGKNRIGFAAKITKNEMIEPAMSGWEGSIAKDTE
                     NFFRLL"
BASE COUNT          426 a          343 c          316 g          207 t
ORIGIN      
        1 caaagaagat aaacggagag aataaggcgc cggacgaaaa agcggacaaa gaaaacaaat
       61 tggcggcaaa acccgcacga ccgcaaacgg ataacgccgc gcccgacaac ggcaccgcga
      121 ggaaagaggc ggacaaaccg attgccgttt ccgaagaaaa gaagtcggcg ccggatattg
      181 ttttacgcgc agaaaaagtt cttccgaaag aaaacgtcgc gcccgcgcta aaaagcggcc
      241 ttgcggaaaa agaaaaagaa ttggaagccc ctcccataaa aacacctccc ggcaagactg
      301 aagcggacaa aaataaatcc gttcccaacg ataccgtccc ggccataaac tccgccagcc
      361 ggcaaaattc cggcgaaaca aaaacaccgt caaaagaaga aatcgccgaa acaacaggac
      421 gcaaggcaac atctatcgcg aacaggtgga agccgcgccg gcaaggaata tccgggtttt
      481 cgcccaagaa aattcgccga aaatcgtacc gacggcggaa gcggaaagag aaatgcccaa
      541 cctcgtaaaa aacgaacccg tccggcccaa tccctcggaa aacgaacccg aaaccgaccg
      601 cggcaacctt gcgccgaatc cggaaaatcg atggcggcaa atcaaaaaaa ccaccctgcc
      661 cggcgccgcc gcatttgcgc cgccggacgt caaagcggag gaaagccggg cgcaaatggc
      721 aacggaaaga ccggcggcaa aatccaaaag aaataatctg ttggcaagat tactgatcat
      781 ttttgcggtg attgccgttt tgggaatcat tttggcagtc attctgatca aaaacggcgg
      841 gaaaacgccg gcggtcgtta aaagcgagcc aaaaccgacc atgacgacgg agaaaaatct
      901 gccggaagaa cccgccgcgc cggcgccgat agtaacaaca ctgacaacgg tgacaattta
      961 tgttgacagt cccgccaata tccccagttc aatttctcct tatatgcgga aaaaatttga
     1021 cgctaacggt tatcactcgc tgttaatcgt tgacagaggc accggcaaaa agatcggctt
     1081 gaaacagttt tttagcgcct acaaattgaa cgcgccgtcg attttttata actcgataga
     1141 agatgactgc gaatttttca tttatgccaa caacggcaaa aaccgtatcg gtttcgccgc
     1201 caaaatcacc aaaaatgaaa tgattgagcc cgcaatgtcc ggctgggagg ggagcatcgc
     1261 caaagacacc gaaaatttct ttcggctgtt gg
//
LOCUS       DAOQWE010000219         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_138295_length_1282_cov_1.535452, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000219 DAOQWE010000000
VERSION     DAOQWE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138295_length_1282_cov_1.535452"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKK37_04880"
     CDS             <1..878
                     /locus_tag="PKK37_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81744.1"
                     /translation="ATFHFTTFDNPFIARGELEKTKKEMDADTYRQEVLAEYVDNAGA
                     LFRYDALVDVFSNSISKTAEKYLAVDIAEDGSDKTIFSFWQGLEEYRRERFERMNTET
                     IVSQIREYAAQERIPYSQIVVDAIGVGAGVASNSMLDGIIGYKSSYQAFKTDSDIVKL
                     PNINYTAKAPALISDYKNLRSQCIFTLADLVNNHKISSRTSGRDKEAVIEELANYQDA
                     SKGDGKRMATQKDEVKEIIGRSPDDSDCWIMRMYFELKGKLLPQQSEEVARIISLQKT
                     RFAINREKLKSYSNR"
     gene            878..>1282
                     /locus_tag="PKK37_04885"
     CDS             878..>1282
                     /locus_tag="PKK37_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81745.1"
                     /translation="MAELSIQQNSDIGALVRKTEQEFVSGTTHRSKYVDTSLYNDINT
                     IDAYLNSVHISGKNDNLGREKPFYNIVKMARNVWYRATDIDRKNIIVSPTKTKDTLLS
                     FFATIKLQKWMKTADFGTFLNRWGLVLAAYNSA"
BASE COUNT          405 a          230 c          334 g          313 t
ORIGIN      
        1 ttgcgacatt ccattttaca acttttgata atccgtttat tgctcgcggt gaattggaaa
       61 aaacaaaaaa agaaatggat gccgacacct accggcaaga agtattggcc gagtatgtgg
      121 acaacgcggg tgcgctattt cgttatgacg cgctggtgga tgtattcagc aattcaattt
      181 ccaaaactgc cgaaaagtat ttagcggtgg atattgccga agacggaagc gataaaacga
      241 tattttcttt ttggcaggga ttggaagaat atcggaggga aagatttgaa cggatgaata
      301 ctgaaacgat tgtgtcgcaa atcagagagt atgccgcgca agagcggata ccatacagcc
      361 agattgtagt ggacgcgatt ggagtggggg cgggtgtggc cagcaattca atgcttgatg
      421 ggattatcgg atataagtcc agttatcagg cgttcaagac cgatagcgat attgtgaagt
      481 tgccgaatat caactatacc gccaaagccc ccgcgttgat aagcgattac aagaatttaa
      541 gaagccagtg catttttacc cttgcggatt tggtcaataa ccacaagatt tccagcagga
      601 ctagcgggcg ggacaaagaa gcggtgattg aggagttggc caattatcag gacgcaagca
      661 agggtgatgg caagcgaatg gcgacccaaa aagacgaagt aaaagagatt attggcagaa
      721 gcccggacga cagcgactgt tggataatga gaatgtattt tgagcttaaa ggcaagttgt
      781 tgccgcagca aagtgaagaa gttgcacgaa ttatctctct tcaaaaaaca agatttgcga
      841 taaacagaga aaaattaaaa tcatattcaa atagataatg gcagaattat caatccaaca
      901 gaatagcgac attggcgcgc tggttcgtaa gaccgaacaa gaattcgtta gcggcacgac
      961 gcaccggagc aagtatgtgg acaccagtct ttataatgac atcaacacga ttgacgctta
     1021 tttgaattcc gttcatatca gcggcaagaa tgacaatttg ggaagagaga agccgtttta
     1081 taatattgtg aagatggcgc gcaatgtgtg gtatcgcgcg accgatattg accgaaagaa
     1141 tatcattgtt tctccgacaa aaacaaaaga tactttgttg tcattttttg cgacaatcaa
     1201 attgcagaag tggatgaaga cagcggactt tggcacgttt cttaaccggt gggggttggt
     1261 gttggcagct tataattcgg cg
//
LOCUS       DAOQWE010000220         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_140034_length_1272_cov_4.099425, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000220 DAOQWE010000000
VERSION     DAOQWE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140034_length_1272_cov_4.099425"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PKK37_04890"
     CDS             complement(<1..177)
                     /locus_tag="PKK37_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR81746.1"
                     /translation="MEIIKYGDIDEVVDFLAQGVKEIFGEELVGFYLTGSLSYGDFEP
                     GRSDIDLAAVLEVPA"
     gene            complement(213..620)
                     /locus_tag="PKK37_04895"
     CDS             complement(213..620)
                     /locus_tag="PKK37_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81747.1"
                     /translation="MFEKFKFTAGQIGNYCRSAERDLAIARKSQIPEVIFRFAYDALL
                     KLAIAACAQSGLRAKSRQGHHIELIRKLAEFLDDEDVEILGNQMRSKRNWDLYGGGTI
                     ISAKEAKEYFDWVVKVFAKAQKLFDGPNRRLNL"
     gene            complement(633..1181)
                     /locus_tag="PKK37_04900"
     CDS             complement(633..1181)
                     /locus_tag="PKK37_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNR81748.1"
                     /translation="MISLRSKIAVKVLGYFFLNPESERYINELAKILSLDPGNLSRKL
                     NELEKQGIISSRRFGNRRHYFLNKKYPLLREVKKIFDVSFGLPRLVQNKIKNLKGLDQ
                     AYIFGSFPRGETAGDSDIDLLLVGSHSALEAKKNLLSLEKEIGREINVVDMGQKEFER
                     ARIGGDPFIKNIFSDKIIELAV"
BASE COUNT          357 a          323 c          221 g          371 t
ORIGIN      
        1 tgccggaact tccaaaaccg ccgccagatc gatatcgctg cgtcccggct caaaatcgcc
       61 ataggacaac gacccggtca aataaaaccc gaccaattcc tcgccgaaaa tctcttttac
      121 cccctgcgcc aaaaaatcaa ccacctcatc aatatcccca tattttataa tttccatttt
      181 taccgccata tattttttat attctttcga gactacaaat tcaagcggcg attggggccg
      241 tcaaacaact tttgcgcttt ggcaaacact ttgacgaccc aatcgaaata ttccttggct
      301 tctttggccg aaattattgt gccgccgcca tacaaatccc aattgcgctt tgaccgcatt
      361 tggttgccca aaatctcaac atcctcgtca tccaaaaatt ccgctaattt gcggatcaat
      421 tcaatatgat ggccttgtct tgattttgcc cgcaaaccgc tttgggcgca cgccgcaatt
      481 gccaatttca ataatgcgtc ataggcgaac cgaaaaatta cttccggaat ttgtgatttg
      541 cgggcaatcg ccaagtcccg ttccgccgac cggcaatagt tgccaatttg gcccgcggta
      601 aatttgaatt tttcaaacat tgaagctgtt gctcaaaccg ccaattcaat tatcttgtcc
      661 gaaaaaatat ttttgatgaa tgggtcgccg ccgatccggg cgcgctcaaa ttccttttgc
      721 cccatgtcaa caacattaat ctcgcggcca atttcttttt ccagcgacaa aagatttttt
      781 ttggcttcca gcgcggaatg ggaaccgacc agcaaaagat caatgtcgct gtctccggca
      841 gtctcccccc tggggaaaga accgaaaata taagcctggt ccaatccttt caaatttttg
      901 atcttgtttt gcaccaatcg aggcaaaccg aaactcacat caaaaatttt tttgacttcg
      961 cgcagcaagg gatatttttt attcaaaaaa taatggcgcc ggttgccaaa acgccggctg
     1021 gaaataattc cttgtttttc caattcattc aatttgcgcg aaagattccc cggatccagc
     1081 gacaaaattt tggccaattc gttgatatat ctttcacttt cgggattcaa aaagaaatat
     1141 cccaaaacct tcaccgcgat ttttgatctt aatgagatca tttggttgat attattgtat
     1201 ttacttacaa taattattgt aaattattac aatgatttgt caacctccgg taaaaacttc
     1261 attaaaggat ag
//
LOCUS       DAOQWE010000221         1265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_141374_length_1265_cov_7.710744, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000221 DAOQWE010000000
VERSION     DAOQWE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141374_length_1265_cov_7.710744"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1032
                     /locus_tag="PKK37_04905"
     CDS             124..1032
                     /locus_tag="PKK37_04905"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNR81749.1"
                     /translation="MFTSLRRVIGLGWQNLARDGGIAAANVFIIMIPILLTTAIFSLN
                     AAGDFLIKQLQGRADISVYFNESVAEEDILRVQGEIVRIPGIEKADYVSRERALEDFR
                     LRYESDQTLMESLEEVGANPFPALLHVTADSPAQFEEVALMLERDDYRDMIYKLNYNE
                     RKENIGKIFAFTENAKKIGLWLFVLLASISVLVTFNTVRMAIMSRKREIGIQRLVGAS
                     RWFIRGQFLVEGMIFGVLAAVFSFCVSLLVCHYAGPALAGVMSGMNLWEHYNANIFTI
                     LGMQSGIGVGLGALSSLIAVGRYLKI"
     gene            1147..1217
                     /locus_tag="PKK37_04910"
     tRNA            1147..1217
                     /locus_tag="PKK37_04910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1179..1181,aa:Gln,seq:ctg)
BASE COUNT          280 a          276 c          362 g          347 t
ORIGIN      
        1 cgcgcgaaat ctgaaaattt cgttgcgtaa atatttacgc agtaggtcta attttaaaat
       61 ttgccggttt tatattttga attgtcattt tgaattttga tttttaaatt ttaaatttga
      121 aacatgttca cttctcttcg gcgagttatc ggtttgggtt ggcaaaattt ggcgcgcgac
      181 ggcgggattg ccgcggccaa cgtgttcatc atcatgatcc cgattttgct caccacggcg
      241 attttttcgc tcaacgccgc gggtgatttt ttgatcaaac aactgcaggg gcgggccgat
      301 atttccgttt acttcaatga aagtgttgcc gaagaggata ttttgcgcgt gcagggtgag
      361 atcgttcgga ttccgggcat tgaaaaagcg gattatgttt cgcgcgaacg ggcgttggaa
      421 gatttccgcc tgcgctacga aagcgaccaa accttgatgg aatcattgga agaggtgggc
      481 gccaatccgt ttcccgcgct cttgcacgta accgcggact ctcccgcgca atttgaggaa
      541 gtcgcactga tgctggaaag ggacgattac cgcgatatga tctacaagct caactataac
      601 gaaaggaaag aaaacatcgg gaaaatattc gcattcacgg aaaacgcgaa gaaaatcggg
      661 ctctggctgt ttgttctgct ggcgtcaatc tccgtgctgg taacgttcaa caccgtgcgg
      721 atggcgatta tgagccgcaa acgggagatc ggcatccagc ggctggtggg cgcgtcgcgc
      781 tggttcatcc gcgggcagtt tctggtggaa ggaatgattt tcggggtgtt ggcggcggtg
      841 ttctcgtttt gcgtgtcgct tttggtttgc cattatgccg ggccggcgct ggcgggcgtg
      901 atgtcgggga tgaatctgtg ggagcattat aatgccaaca ttttcacgat tctgggaatg
      961 cagtcgggca tcggcgtcgg attgggcgcc ctttccagtc tcatcgcggt gggccgctac
     1021 ttgaaaattt aacgcaatcc gctagaatgc gaattgccgg atccaagatt caagattcaa
     1081 gattgcaaat cccggttttg ttggtttttg cgctgatttt ttattttgat attcggattt
     1141 aactaatgcg ggatcgtcta acggtaggac gacggactct gaatccgtta atcatggttc
     1201 gaatccatgt cccgcagcaa gcgaccgaag gggagcgcag tgcccagcgc cagcaggcgg
     1261 cgggt
//
LOCUS       DAOQWE010000222         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_141951_length_1262_cov_4.677713, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000222 DAOQWE010000000
VERSION     DAOQWE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141951_length_1262_cov_4.677713"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PKK37_04915"
     CDS             <1..732
                     /locus_tag="PKK37_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81750.1"
                     /translation="PKEIVDSRVRVCDAAKLRLVAMELESQAAVRSARQNANFAWPAI
                     IIKIGDTQSSVIFCGRDSTRAVFSVPISNAYFIEKIRAAAEVDDDGARKLKTDCGIAR
                     AAGFDAPPKGDDNDKREKLFESLLPGLVDFAQQIKKYVRYYQEYEKSFRADKLSFRKV
                     IICGDGSDLKGLDDFLSLKLGVPVERMVLPIGIEPHRFKSVDSPEKSAHGCAVAAGLA
                     ARALAIESARGGGRPPKPRFQKKQK"
     gene            729..1259
                     /locus_tag="PKK37_04920"
     CDS             729..1259
                     /locus_tag="PKK37_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81751.1"
                     /translation="MINLLPPLRQKQIAEEEAAKVAAIVVMAATAALLFFAAALFVVR
                     IFYQYELKTAEIIMEEKKAIAGMRDVSAVESQIALGNALAAEITGFDRRQTKITEVFL
                     RVAECLPAGISLSGFQFSQNRINIEGTSPNRDILALFKKNLESRSDFTKIVFPASNWL
                     SASDIEFSASFEYVKN"
BASE COUNT          308 a          297 c          349 g          308 t
ORIGIN      
        1 cccaaagaaa ttgtggattc ccgcgtccgc gtttgcgatg ccgcgaagtt gcggctcgta
       61 gcaatggaat tggaatcgca ggcggcggta agatcggcgc ggcagaacgc gaatttcgcc
      121 tggccggcaa tcatcatcaa gatcggcgat acgcaatcaa gcgtgatttt ttgcggccgt
      181 gattccacgc gcgcggtgtt ttcggtgccg atttccaatg cttacttcat tgaaaaaatt
      241 cgcgccgccg ccgaagttga cgatgacggc gccagaaaat taaaaaccga ttgcggaatt
      301 gcccgcgcgg cgggttttga tgcgccgccg aagggcgatg ataacgacaa acgggaaaaa
      361 ctttttgaat ctttattgcc cggattggtt gatttcgccc agcaaatcaa aaaatatgtc
      421 cgctattatc aggagtacga aaaatctttc cgcgccgata agctgtcttt ccgaaaagtg
      481 attatctgcg gcgatggttc cgatctgaaa ggtcttgacg attttctgtc tttgaaatta
      541 ggagttcccg tggaaaggat ggtattgccg atcgggattg aaccgcaccg gttcaaaagc
      601 gttgattctc cggaaaagag cgcacatggt tgcgcggtgg ccgccgggtt ggcggcaagg
      661 gcgctggcga ttgaatcggc gcgcggcggc gggcggccgc cgaaaccgcg ttttcaaaaa
      721 aaacaaaaat gatcaatctt ctgccgccgt tgcggcaaaa acaaattgcc gaagaggaag
      781 cggcaaaagt tgccgcgatc gttgtgatgg ccgctactgc cgcgctgttg ttttttgccg
      841 ccgcgctgtt tgtggtccgc attttttacc aatacgaatt aaaaaccgcg gaaattatta
      901 tggaagaaaa aaaggcgatt gccggtatga gagacgtttc cgcggtggag tcgcaaatcg
      961 cgcttggcaa cgccttggcc gctgaaatca ccggttttga ccgccggcaa acaaaaatca
     1021 ccgaagtgtt tttgcgcgtt gccgaatgtt tgcccgcggg gatttccttg tccggttttc
     1081 aattttcgca aaaccggatc aatattgagg ggacttctcc caatcgcgac attttggcgc
     1141 tgttcaaaaa aaatctggaa agccggtccg attttaccaa aattgttttt ccggcgtcca
     1201 attggctgtc ggccagcgac atagagttta gcgcgagctt tgaatatgtt aaaaattaac
     1261 cg
//
LOCUS       DAOQWE010000223         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_142517_length_1259_cov_3.304817, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000223 DAOQWE010000000
VERSION     DAOQWE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142517_length_1259_cov_3.304817"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1259
                     /locus_tag="PKK37_04925"
     CDS             <1..>1259
                     /locus_tag="PKK37_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNR81752.1"
                     /translation="IVCRGKAEIVQNEKTDRYQIIITEIPYQVQKSNLLEQIAKLVSE
                     KKIDGVKDVRDESDKDGLRVAIDLNRDAQPQKILNRLYKFTDLQRTFHLNMLALVDGI
                     QPQVLNLAETLNYFLEHRKQVVARRIQFELDRAKERAHILEGLVKCLADIDEVIRIIR
                     KSENREDARNNLMKRFKLDEIQANAILETKLAALAKLERQKIDNELKELMAKIAEYLG
                     ILKSPAKIKAVVKKELAKAKETYGDERRTKVYAHKIGEISEADLIPDEEVAITLTNSG
                     YIKRIDPKTYKIQKRGGKGIIGLKTVGDDIVEQSLIASTHDRILFFTDMGRVFQTLAY
                     EIPEGARVAKGRALVNFLDLGPQEKVLYLMSLGSASAKAMADKAGKGKVKNNGNGQSE
                     ESKYLLIVTKNGIAKKTATEDFDNIRR"
BASE COUNT          373 a          294 c          336 g          256 t
ORIGIN      
        1 gattgtttgc cgcggcaaag ccgaaatcgt gcaaaacgaa aagaccgatc gctaccaaat
       61 tatcatcacc gagattccct atcaggtgca aaaatccaat ctgctggaac agattgccaa
      121 gttggtgtcg gaaaaaaaga ttgacggcgt caaagatgtc cgtgatgaat ccgacaagga
      181 cggcctgcgc gtggcgattg atctcaaccg cgatgcccag ccgcaaaaaa tcctcaaccg
      241 gctttacaaa ttcacggatc ttcagcgcac cttccatttg aatatgctgg cactggtgga
      301 cggcatccag ccgcaagttt taaaccttgc cgaaacgctg aactattttt tggagcatcg
      361 caagcaagtg gtggcgcgcc gcatccaatt tgaacttgac cgtgccaaag agcgggcgca
      421 tattttggaa gggttggtga aatgtctggc cgatattgac gaagtgatcc gcatcatcag
      481 aaaatccgag aaccgcgaag acgcgcgcaa caatttgatg aagcgattca agcttgacga
      541 gatccaggcc aacgcgattc tggaaaccaa gctggcggcg ctggcgaaac tggaacggca
      601 gaagattgac aatgaattga aggaattgat ggccaagatt gccgaatatc tcgggatatt
      661 aaaaagcccg gccaagatca aggcggtggt caaaaaagaa ctggcgaaag ccaaagaaac
      721 ctatggcgat gagcggcgca cgaaagttta tgctcacaag atcggcgaaa tttccgaagc
      781 cgatttgatt cccgacgaag aagtggcaat cactttgacc aattccggct acatcaagcg
      841 cattgatccc aaaacctaca aaatccaaaa gcgcggagga aaaggcatta tcggcctcaa
      901 aaccgtgggt gacgacatcg tggagcaatc gctgattgcc agcacgcacg accggatatt
      961 gttcttcacc gatatggggc gggttttcca aaccttggcc tatgaaattc cggagggcgc
     1021 gcgcgtggcc aaagggcgcg cgctggtgaa ttttttggat ttgggccctc aggaaaaagt
     1081 gctttatttg atgtctttgg gatccgcctc cgctaaagct atggcggaca aagcggggaa
     1141 aggcaaggtc aagaataatg gtaatgggca gagcgaggaa agcaaatatc tgctgatcgt
     1201 gactaaaaac ggcattgcca aaaaaaccgc gacggaagat tttgacaata tccgccgtt
//
LOCUS       DAOQWE010000224         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_144385_length_1249_cov_5.216918, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000224 DAOQWE010000000
VERSION     DAOQWE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144385_length_1249_cov_5.216918"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="PKK37_04930"
     CDS             complement(<1..1109)
                     /locus_tag="PKK37_04930"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR81753.1"
                     /translation="MLYKREIVDEILKTIDLDEIIVLHGARQVGKTSIMAWLENHLSE
                     SKKKCYYIDLEDSRYKKILDAGTDGFITLSKEEGRFPAHGDRVAVFIDEIQYLQNPSE
                     FLKLVADHHKNIKLIVSGSSSFGIKSKFKDSLAGRTFNFEIFNLSFLEYLEFKNYYYD
                     PAAAATEKKISELKSFYKEYVLFGGYPKITLTDEIDLKEKRLWQIIDTYVKKDIAEIG
                     GIGNVESFNKLLEVLAAQGGQLVNTNELAATCKLARATVEKYLFILEETYIIKLVRPY
                     SGNIRSEISKMPKVFFYDTGIMQMLWLKSLQKEIIGNVFETSVFAELAKARGRQNIYY
                     WRTKDKKEIDFIVKDKNIILPIEAKLNFSRADLTP"
BASE COUNT          341 a          266 c          233 g          409 t
ORIGIN      
        1 attggggtta aatccgcccg gctaaaattg agcttggcct caatgggcaa gattatgttt
       61 ttgtccttga cgataaaatc aatttctttt ttatcttttg tgcgccaata ataaatattt
      121 tgccggccgc gcgctttggc caattcggca aagacgctgg tttcaaaaac attgccgatg
      181 atttcttttt gcaagctttt gagccaaagc atttgcataa ttccggtgtc atagaaaaac
      241 actttgggca ttttggaaat ttcactgcgg atattgccgc tatacggcct gaccaatttg
      301 atgatgtagg tttcttccag aataaacaga tatttctcca cggtcgccct cgccaatttg
      361 caggtcgcgg ccaattcgtt ggtattgacc aattgcccgc cctgcgcggc cagcacttcc
      421 aaaagcttgt tgaagctttc cacgttgccg attccgccga tttcggcaat gtcttttttc
      481 acataagtgt caataatttg ccaaagccgc ttttccttta aatcaatttc atccgtgagc
      541 gtaatcttgg gatatcctcc gaagagaaca tattctttgt aaaatgattt caattcggaa
      601 attttctttt cggtagcggc cgccgctggg tcgtaataat aatttttaaa ctccaaatat
      661 tccaaaaagg acagattgaa aatttcaaaa ttaaacgttc ttccggccaa agagtcttta
      721 aatttgcttt taatgccaaa gctggacgat ccggaaacaa tcaacttgat gtttttgtga
      781 tgatcggcga ccaatttcaa aaactccgaa ggattttgga gatattgaat ttcgtctatg
      841 aaaaccgcca ccctgtcccc atgagcaggg aagcgcccct cctcttttga gagagttata
      901 aatccatcgg tgccggcatc aagaattttc ttatatcttg aatcttcaag atcaatgtag
      961 tagcatttct tttttgattc ggacagatga ttttccagcc acgccataat tgaagttttc
     1021 cccacttggc gggccccgtg caaaacgatt atttcgtcaa gatcaatggt ttttaaaatt
     1081 tcgtcaacaa tttccctttt gtaaagcata ttttttacct ggattttaga tatagtttga
     1141 ttatagttca actaaaatat aaagcaagtt ttgtttttgg ttggacaaaa atctctcatc
     1201 ttgccgtttt taagcaaaaa caccgcaaat tgcaacctga ctttacaat
//
LOCUS       DAOQWE010000225         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_145540_length_1243_cov_13.617845, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000225 DAOQWE010000000
VERSION     DAOQWE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145540_length_1243_cov_13.617845"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKK37_04935"
     CDS             complement(<1..598)
                     /locus_tag="PKK37_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNR81754.1"
                     /translation="MKTKYFLYARKSTEDEERQVMSIEAQLAELADFAKRENIEISEQ
                     FIESKSAKKPGREFFNEMIQKIYESKEPVGILAWHPDRLARNSVDGGQVIYLIDIGKI
                     ASLRFPTFWFEPTPQGLFMLQVAFGQSKYYSDNLSENVKRGIRQKLRRGEWPGLAPFG
                     YVNNPKTRTIEPDQMKARVIKKAFEEYAQGRHTLESLGQ"
BASE COUNT          289 a          311 c          293 g          350 t
ORIGIN      
        1 gttggcccaa actttccaaa gtatggcggc cttgcgcgta ttcctcaaac gctttcttga
       61 taactctagc tttcatttgg tcgggttcta ttgtgcgggt tttgggattg ttcacatagc
      121 caaacggcgc aaggccgggc cattcgccac gcctgagttt ttgtctaatc ccgcgcttaa
      181 cattttcgga caaattgtcc gagtagtatt tactttggcc aaaggcgact tgaagcataa
      241 aaagaccttg cggagttggc tcaaaccaaa aggtgggaaa gcgcaaagaa gcgatcttgc
      301 cgatgtcaat caagtaaatc acttgtccgc cgtcaacgct gtttcttgct aatctatcgg
      361 ggtgccacgc caaaattccg accggctctt tgctctcgta aattttctgt atcatctcgt
      421 taaaaaattc tcggcccggc ttctttgcgc ttttgctttc aataaactgc tcgctgattt
      481 ctatgttttc tcttttggca aagtctgcca actccgcaag ttgcgcttca atactcatca
      541 cttgtctttc ttcatcttcc gttgatttgc gagcgtagag aaaatatttt gtcttcatag
      601 tgttttactc cttaatttta aataagtcgc ctttggcaat tctaccttcg gcggaaaaag
      661 cgcgggatta aaaactggct tcgtggtgtc gcaaagcggc acgcggaaaa aaattggaaa
      721 gcaaaaacat ttcaataaat ttcttatagg cgtgtttttg ctctggcggc ttcgccgccc
      781 cgaaccgccg cctgctggat agaaaactga tttttgattt tgtccgcccg tttgattttg
      841 tgccgaaata tacggaaaaa tgtgagcgaa gccccgccgc aggcggggcg agcgagcaaa
      901 caaactgccc gcaaaattcg caatgtttag attggtcgca attactgaac gaagtgcgaa
      961 cccattttga gcaagaattt ctgccctgac gggcagaaac ccgccccagc ggctcgccgc
     1021 ctgacggcgg acaaagcccg caaaaatttc tttttctttt aatgggcgcg cgcaaaattc
     1081 tttttcaatt aaggaatcaa tttttgcggg ctttgcttcc ttgctgacgc aaggacgagc
     1141 cgcttcccac cccaacgctc gggcggggcg ggaaggaaag ggggttgggg ggaaaggaat
     1201 tcccgccccg ccctcgcttt ttcgccgccg caatttttcg tgc
//
LOCUS       DAOQWE010000226         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_147107_length_1235_cov_4.622034, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000226 DAOQWE010000000
VERSION     DAOQWE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147107_length_1235_cov_4.622034"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKK37_04940"
     CDS             complement(<1..508)
                     /locus_tag="PKK37_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81755.1"
                     /translation="MDCEKESSIGKIKTSLCEVLILTETIKKISNFAVLWLNIGITCN
                     RRMKYFIQIFGCQMNISDAQRIAGLFESRGMKEASTAEAADVFVAVMCSVRQMAADRV
                     FGLEQKIKSVKKKNKKITSVLTGCVVESDKPRFAEIFDHILDIKTLPRWPKILHLARG
                     KSRESLTFA"
     gene            complement(533..1165)
                     /locus_tag="PKK37_04945"
     CDS             complement(533..1165)
                     /locus_tag="PKK37_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81756.1"
                     /translation="MIQKKKSLAFCAAIVAGGAFLLGSGFASAQTTVVNTGTATSTIT
                     GTIDISGLDKQKMLTQIQAAISNIQQTLTTILINYAAIQQQHIQNLTNSAAQQITGSG
                     SATSNPLGGLLSGGTGSGIGSFVTGSGNATTNVTGNAYTNVNGTLTENSGTVSGDTSP
                     GQSTITNSGTISGSTPINTTAIQQQISTIQNQIMSIFSQIGGGSTASTGN"
BASE COUNT          286 a          271 c          302 g          376 t
ORIGIN      
        1 ttgcgaaggt tagactttcg cgggacttcc cgcgcgccag atgcaaaatc tttggccacc
       61 ggggaagggt tttaatgtct aaaatatggt caaaaatttc ggcaaaacgc ggtttgtcgc
      121 tttccactac gcaaccggtt aaaaccgagg taattttttt gtttttcttt ttgacggact
      181 taattttttg ctccaacccg aaaacgcggt cggcggccat ttgccgcacc gaacacatta
      241 ccgccacaaa aacatccgcc gcttccgccg ttgaggcctc tttcattccc cgcgattcaa
      301 aaagtccggc gatgcgctgg gcgtcggaaa tgttcatctg gcaaccgaaa atttggataa
      361 aatacttcat tcgtctatta caggtaatac caatatttaa ccacaaaacg gcaaaattgg
      421 aaattttttt aatcgtttca gttaaaatta aaacttcgca aagcgaagtt ttaattttgc
      481 cgattgagct ttccttttcg caatccatcg ggcgttgttt ttgctcgtca ggctaattgc
      541 cggtggaagc ggtgctgccg ccgccaattt gcgagaaaat gctcattatc tggttttgta
      601 tcgttgaaat ctgttgctgg atggcggttg tatttatcgg ggtggaaccg gaaatcgttc
      661 cgctgttggt gatggttgac tgtccgggag aggtgtcgcc cgagactgtg ccggaatttt
      721 ccgttaacgt tccattaacg ttggtgtagg cgtttccggt aacattcgtg gttgcgttgc
      781 cgctaccggt gacaaaactg ccgatacccg aaccggtccc gccggaaagc agacctccca
      841 aaggatttga ggttgcggag ccgctaccgg ttatttgctg ggcggccgaa tttgtcaaat
      901 tttgaatgtg ctgttgctgt atggcggcat aattaatgag gattgtcgtt aatgtttgct
      961 ggatattgct gatcgcggcc tgtatttggg tgagcatttt ctgcttgtca agtccggaaa
     1021 tgtctattgt gccggtgatc gtgctggttg ccgtgccggt attcacgacc gtggtttggg
     1081 ccgaagcaaa accgcttccc aaaagaaatg cgccgccggc aacaattgcc gcgcaaaaag
     1141 caagagattt ctttttttga atcatatttt attttttaaa ttaattttta cttataatat
     1201 tttttgttaa caaacgattg cgtcgaccat tatcg
//
LOCUS       DAOQWE010000227         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_148754_length_1227_cov_7.345563, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000227 DAOQWE010000000
VERSION     DAOQWE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148754_length_1227_cov_7.345563"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..873
                     /locus_tag="PKK37_04950"
     CDS             247..873
                     /locus_tag="PKK37_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81757.1"
                     /translation="MGKIEKVWILSICIFLILAIWWAITFPATYSIPLDQSADKALAA
                     ADITIFENYKGTQIKINETDVNNTILCSNLNTFIREVGNGTVIKGIAISPASGRLTVT
                     TTYLHPRLLAENYNMYSKPTAVVKYVTAKTIVAPDYYEGASAASVSASEVKIWRNSIS
                     MSIGVMFWTIIFAILSAFLLMTAEWCWDKCKVIKRKATRAIKGLISKK"
     gene            complement(1019..>1227)
                     /locus_tag="PKK37_04955"
     CDS             complement(1019..>1227)
                     /locus_tag="PKK37_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81758.1"
                     /translation="RIFLITLRGKSNPEMKVGAEGPRHGEEPYPAPLIYVLENFIPQG
                     YLDYNQPQNLVNPTYPKLLKQNKP"
BASE COUNT          347 a          222 c          283 g          375 t
ORIGIN      
        1 ccgtcataca gcctgcaagc gccggcggtg gccgtgtcct tgatcaagca attggcgcaa
       61 accctgggta tttgaatttt gtattccaag ggtctaaccc ccgcgtaatc gcgggggtta
      121 gaccctgcca aactgtattt gaatttattt taatcccgcg tttgcgtgat ttttgattat
      181 gttgctacgc tattgacaat ttataaaaat aatgtattat ttttttggaa tggaggtcgg
      241 tttataatgg gaaagataga aaaggtttgg atattgtcaa tttgtatttt tttgattttg
      301 gcgatatggt gggcgataac ttttccggca acatattcaa ttcctttgga tcaaagcgcg
      361 gacaaggcgc ttgccgctgc cgatataacg atctttgaaa attacaaagg cactcaaata
      421 aaaatcaatg agacggatgt caataataca atcctttgtt ccaacttaaa tacttttata
      481 agagaagttg gtaatggaac tgtaattaag ggcattgcga tttcccctgc gtcaggtcgc
      541 ttgacggtta cgacgacata tcttcacccg aggttgttgg cggaaaatta taacatgtat
      601 tcgaagccga cagcagttgt aaaatatgtt acagccaaga cgatagtcgc gcccgattat
      661 tacgaggggg catcggcggc aagcgtgagc gccagcgaag ttaagatttg gcgaaatagc
      721 atctcaatgt caattggcgt aatgttctgg acaataatct tcgctatttt gtcagccttt
      781 ttgctgatga cagcggagtg gtgctgggac aaatgcaaag tgatcaagcg gaaagcaacc
      841 agggccataa agggattaat atccaaaaag taaataaaga ttaacaacct ttatttttta
      901 tttcaaaatt tgcgcggtta ctgccgcatc tgggtaaatt tccccaaatg cttgtttcgc
      961 aattcaaagc aatttaatcg tgaatttatg aaaaaaccgg attttaactt ggtgaaaatc
     1021 agggtttatt ttgttttaat aatttgggat aggtgggatt gacaaggttt tgaggttggt
     1081 tataatcaag ataaccctgt ggtataaagt tttccaacac atatatcagg ggggccggat
     1141 atggttcttc gccgtggcgg ggaccttcgg cacccacttt catttcggga ttactttttc
     1201 ctcgcagggt tatcaaaaaa atcctta
//
LOCUS       DAOQWE010000228         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_149240_length_1225_cov_3.219658, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000228 DAOQWE010000000
VERSION     DAOQWE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149240_length_1225_cov_3.219658"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PKK37_04960"
     CDS             complement(<1..365)
                     /locus_tag="PKK37_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81759.1"
                     /translation="MSDMSNSEKNDNGSACERRAGVAIYIAAASMSIVLAAALGVGLM
                     VAGRIKMLNEAGDSVKAFSLAQIGVEDALFKQIDSAGYSYSWTDGDSRYEVTSYVCGE
                     FVCIQSTGKYRNANRAIRIR"
     gene            complement(386..1015)
                     /locus_tag="PKK37_04965"
     CDS             complement(386..1015)
                     /locus_tag="PKK37_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR81760.1"
                     /translation="MITCNLSSINQMKPGKKLNKIGQCGFTLVEMIVTLAIFSLIMVA
                     VAGLFFHALRAHRTILARSQMLGESSYNLEHIGRGLRMAKKSPDGSCLSSKGLNFEKT
                     ARGGVKFQNLNSSGTTDCVEYYLGHPGGSYGANGALMESRANAERSFDLPLTSPAVNV
                     MSFDVYDYGWAQNDDLQPRTTIHLRMQGRQNQTMDHQITISQRDLDVEE"
BASE COUNT          318 a          325 c          264 g          318 t
ORIGIN      
        1 cgaatcctga tcgcccggtt cgcgttccga tatttacccg tggattgaat gcaaacaaac
       61 tcgccgcaaa cgtatgacgt cacctcatag cgggaatctc cgtccgtcca gctgtaagaa
      121 tagccggccg aatcaatctg cttgaacagg gcgtcctcca cgccgatctg cgccaaagaa
      181 aacgccttaa ccgaatcgcc cgcttcgttt aacattttaa tccggccggc caccatcaat
      241 cccacgccca acgccgccgc cagaacaatt gacattgacg cggcggcgat gtaaatcgcc
      301 acgcccgccc ggcgttcgca ggccgaaccg ttatcgtttt tttcggaatt ggacatatcg
      361 gacatatatt gaatttgcaa aataatcact cttcaacatc aagatcgcgc tgggaaatcg
      421 tgatctgatg atccatggtc tggttctggc gcccttgcat ccggagatga atcgtggtcc
      481 ttggttgcaa gtcatcattt tgcgcccagc catagtcata cacatcaaag gacatcacgt
      541 tcaccgccgg tgaggtgagc ggcagatcaa aagacctttc cgcgttggcg cggctttcca
      601 tcagcgcgcc gttggcaccg tagcttcctc ccggatggcc cagataatat tccacgcaat
      661 cggttgttcc ggaagaattc aaattttgga atttcacgcc gccgcgggcg gttttttcaa
      721 aattaaggcc cttggatgaa agacaactgc cgtccggcga ttttttcgcc atccgcaagc
      781 cccggccgat atgttccaaa ttataggaag attctccgag catctgcgat cttgccagaa
      841 tggtgcgatg agcgcgcaag gcgtgaaaaa aaagcccggc cacggcgacc ataatcaaag
      901 aaaaaatcgc cagcgtcacg atcatttcca ccaaagtgaa cccgcattgc ccgattttgt
      961 ttaatttttt ccccggcttc atttgattga ttgatgataa attacaagtg atcattgata
     1021 atcaataatt gatcatcaat aattgatcat caataattga tcattgatca ttgatcattg
     1081 atcattgata acttatcatt tatcatttat tacttatcaa ttatcaatga ttaatgattg
     1141 atgattaatg atcactgcca ccagttatac aatctctctc tgacggtttc ggtgtgctgg
     1201 ccggccgaat cggaccacga aactt
//
LOCUS       DAOQWE010000229         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_150100_length_1221_cov_2.727273, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000229 DAOQWE010000000
VERSION     DAOQWE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150100_length_1221_cov_2.727273"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..380
                     /locus_tag="PKK37_04970"
     CDS             27..380
                     /locus_tag="PKK37_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNR81761.1"
                     /translation="MELNEFEKLTAMAVDGLPQNIKDALKNVAIVIEDGPAKRNLLGL
                     YEGIPENQWGKSDAIRLPDKITIFKSLIEEEARTPEEIKELVRVVVWHEIAHHFGFDE
                     PQTRRLERRWRARKP"
     gene            complement(600..>1221)
                     /locus_tag="PKK37_04975"
     CDS             complement(600..>1221)
                     /locus_tag="PKK37_04975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR81762.1"
                     /translation="TPPWVRIERIIRDIPSPRITTGTKGISNLRQIIANDMEREHWRC
                     QCIRCREIKGDYNPQEKIILVRRDYQASQGKEIFLSFENEDKTKLFSLLRLRITDKGT
                     KLMIENAGDYMESPLRNTNTIGIIREIHTYGIQTAIGKIATSAQHTGLGKKLIKEAER
                     IAKTEFNIKKIAAISGVGARQYWRKNGYKLQNTYMVKKLRERIRAC"
BASE COUNT          305 a          262 c          311 g          343 t
ORIGIN      
        1 aactctaaaa ctctgatcaa tttgctatgg aattaaatga atttgaaaaa ctgactgcca
       61 tggccgtgga tggcctgccg caaaacatta aagacgcgtt aaaaaacgtg gcgattgtca
      121 ttgaagacgg gcccgcgaaa cgcaatttgc ttgggttata tgaagggatt ccggaaaacc
      181 aatgggggaa aagcgatgcc attcgcctgc cggacaagat cactattttc aaatcattga
      241 ttgaggaaga ggcgcgcacg cccgaagaaa tcaaggagtt ggtaagggtt gtggtctggc
      301 acgaaattgc ccatcatttc ggttttgacg aaccgcaaac ccgccgcctt gaacgccgct
      361 ggcgcgctcg caaaccttga caaaaataaa aaaatataca tcatcaaatc aggtgattaa
      421 atggtgataa taatttccgg catgtcgccg gaggagaggg gtgatttgga aaagtatgga
      481 caaatcatct ctgaaaaaga aaggacagct cgatttatca ttcggtccaa gcttgaaagg
      541 cttggacgga agcatcggaa aaataaaaat aagagacgcc gccgccgggg cggcggccgc
      601 tagcaggcgc ggattcgttc gcgcaatttt tttaccatat aagtattttg cagtttgtag
      661 ccgtttttgc gccagtattg gcgcgcgccc acgccggaaa tcgcggcgat tttttttatg
      721 ttgaattcgg ttttggcaat tcgctcggct tcttttatca gttttttccc caagccggtg
      781 tgctgggcgg aagtcgcgat tttaccgatc gcggtttgga ttccgtatgt atgtatttct
      841 cttatgatcc cgattgtatt tgtatttcgt aggggcgatt ccatgtaatc gcccgcattt
      901 tcgatcatta attttgttcc tttgtcggtt attcttaacc gcaacaagct gaatagcttt
      961 gttttgtctt cattttcaaa actcaaaaaa atttcttttc cttgcgaggc ttgataatcg
     1021 cgccggacaa gaattatttt ttcttgcgga ttgtaatcgc ctttgatctc gcggcagcgg
     1081 atgcactggc aacgccagtg ttcgcgttcc atatcattgg cgatgatttg gcgcagattg
     1141 gaaattcctt tggttcccgt tgttatgcgc ggggagggga tgtcgcggat gatccgctca
     1201 atgcgcaccc acggcggcgt g
//
LOCUS       DAOQWE010000230         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_150882_length_1217_cov_5.472461, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000230 DAOQWE010000000
VERSION     DAOQWE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150882_length_1217_cov_5.472461"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..285)
                     /locus_tag="PKK37_04980"
     CDS             complement(154..285)
                     /locus_tag="PKK37_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81763.1"
                     /translation="MFIVKGKTNWPMIAAVLVIAAITGGGLMLYINDTIAQTHYLGQ"
     gene            523..>1217
                     /locus_tag="PKK37_04985"
     CDS             523..>1217
                     /locus_tag="PKK37_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81764.1"
                     /translation="MKKTELIFNIILVGGDFLMILAAGSGAYLLRTSPWLRDWRGAGG
                     FFDQLPFGDYLPALLVIAAIWVVIFGLAGLYQTKIDRKYFEDLPKVIVACCAGVMAIT
                     MVVFFRKEFFDSRFIVLAGWVFAVILVSMERAAMKFAWNKYNLGGQKTLLAGNKEIAE
                     KLRRELAANREIDIIATGDSLDLDLEAVKNRFNKDKFDEMILCVPERNREYITAFSNF
                     CEERLIDFRFVPDL"
BASE COUNT          320 a          258 c          309 g          330 t
ORIGIN      
        1 cggcattttc atcgccgccg cgaaaatcaa aaacccccgc taccgcaagc gacactgccg
       61 caatcgccaa gactgctccg gccacggaaa aaacaaacaa aaatttgcct ttcatccgcg
      121 tattattttt ggaaacgcca aaccccgcaa aaatcactgc cccaaatagt gggtttgcgc
      181 gatcgtgtcg ttaatgtaga gcatcaaacc gccgccggtg atcgccgcga tcaccagcac
      241 cgccgcgatc atcggccaat tggttttccc cttgacaata aacatatttt gtttgttaga
      301 cgcctccctg ttcatcatat tataacacgc tttgttttcc cggcaataat gcggagtgca
      361 accactcact gggtttaaaa ttaaaggggt ctatccctcg ttactgcgag ggttagtccc
      421 ctttaatttc gcgcccaaaa tataatcccc agtgagtggt tacgacagag ccgcaatacg
      481 agcgttggga gaacatttga tttctggtat aatctaggaa atatgaagaa aacggaactg
      541 atattcaata ttattctggt gggaggggat tttttgatga tactggcggc gggaagcgga
      601 gcttatttat tgagaaccag tccctggctc agagactggc ggggcgccgg aggatttttt
      661 gatcaactgc cttttggaga ttatctgccg gcgcttttgg tgattgccgc aatttgggta
      721 gtaattttcg gattggcggg attatatcag acaaagattg accgtaaata ttttgaagat
      781 ctcccgaaag tgatcgtggc ttgctgtgcc ggagtgatgg caataacgat ggtggtgttt
      841 tttcgcaagg agttttttga ttcgcggttc atcgtgctgg cgggatgggt tttcgcggtg
      901 attttggtaa gcatggaaag agccgcaatg aaattcgcct ggaataaata taatttgggc
      961 gggcaaaaaa cgcttttggc gggaaacaaa gaaattgcgg aaaaattgag acgggagctg
     1021 gcggcaaacc gtgaaattga tattatcgcg accggcgatt ctcttgatct tgatttggaa
     1081 gcggtgaaaa accgattcaa taaagacaaa tttgacgaga tgattctgtg cgtgccggaa
     1141 cgcaaccggg aatatatcac agctttttcc aacttttgtg aagaaaggtt gattgatttc
     1201 cggtttgttc ccgatct
//
LOCUS       DAOQWE010000231         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_151764_length_1213_cov_4.677029, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000231 DAOQWE010000000
VERSION     DAOQWE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151764_length_1213_cov_4.677029"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1061
                     /locus_tag="PKK37_04990"
     CDS             54..1061
                     /locus_tag="PKK37_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR81765.1"
                     /translation="MQTPSEKTEKMLFATEEEVFKTIIGANHPFRKLNELIDFEKLVE
                     PMRATYSTLGANGIDVQKGIKALLIQFWEDYSDREMEQAIKENLAVRWFCGFSIGETT
                     PDYSYFCKLRKRIGTKRVGQFFKDVNAILDSYGLFGNVFTFIDASTIITKTALWEERD
                     QAIKAGADKLNNSNVQKYAADKDARWGAKSKNHIWFGYKRNTAVDMRCGLIKKVAVTP
                     ANVPDFKAAENVMPDQGAVFMDKIYDTKKTNDIVKAHDCYAATIRKNNNKEKNRDLDS
                     WHSKIRMPFEGTFSKMDKHARYRGLAKNAMQGFLQAAAYNLKKAVNILTVLPTAPIAA
                     V"
BASE COUNT          407 a          271 c          267 g          268 t
ORIGIN      
        1 ctgaatatgg taaaattgaa ttaaggcaaa tccttaattt tcaatcaaaa accatgcaaa
       61 caccttctga aaaaactgaa aaaatgttgt tcgcaaccga agaagaagtt ttcaaaacaa
      121 tcattggcgc caatcatccg ttccgcaagt taaacgagct cattgatttt gaaaagctgg
      181 ttgagccaat gcgcgcaact tactcaacat tgggcgccaa tggcattgat gtccaaaaag
      241 gaatcaaagc gttattaatc caattctggg aagattattc cgaccgggaa atggaacaag
      301 cgatcaaaga aaatctggcc gtgcgctggt tctgcggatt ttcaatcgga gagacaacgc
      361 ccgattacag ctatttttgc aaattaagaa agcgcatcgg aaccaaaaga gtcggccaat
      421 ttttcaaaga cgtgaatgcg attctggaca gctacggtct tttcggcaat gtttttactt
      481 tcattgacgc gtccacgatc atcaccaaaa ccgcgctctg ggaagagcgc gaccaagcaa
      541 tcaaggccgg cgcggacaaa ttgaacaact ccaatgttca aaaatatgcc gccgacaaag
      601 atgcccgctg gggagcgaaa tcaaaaaacc atatttggtt tggctacaag agaaataccg
      661 cggttgatat gcgttgcgga ctaatcaaaa aagtggcggt tactccggcc aatgtcccgg
      721 attttaaagc cgccgaaaat gtaatgcccg atcagggggc ggtgttcatg gacaaaatct
      781 acgacaccaa aaagaccaac gatatcgtca aagcccacga ctgctacgcg gccacgatcc
      841 gcaaaaacaa caacaaagaa aagaatcgcg atctggattc ctggcattca aaaatacgaa
      901 tgcctttcga aggaacattt tcaaagatgg acaaacatgc ccgctatcgc ggactggcaa
      961 aaaatgcgat gcagggattt ttgcaggcgg cggcttacaa cctcaaaaaa gcggtgaata
     1021 ttttaaccgt tttgccaacc gcgcccattg ccgccgttta acgaggatgc agtgcgcccg
     1081 caagacgcaa aaatggcgcg agatgattaa aaaatcaaaa acaatggcaa attaaaagaa
     1141 aaagcaaagt tgtcaacagg taattttgtt aatgactttg cttttccttt taaagccgaa
     1201 tttggcaacg gac
//
LOCUS       DAOQWE010000232         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_151979_length_1212_cov_10.500432, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000232 DAOQWE010000000
VERSION     DAOQWE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151979_length_1212_cov_10.500432"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /gene="grpE"
                     /locus_tag="PKK37_04995"
     CDS             complement(<1..376)
                     /gene="grpE"
                     /locus_tag="PKK37_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNR81766.1"
                     /translation="MEKEIEVLQKELDEVKKQAEANLNGWKRAKADLINYKKEESQRM
                     EEFGKYNRQAFVFSLLPIMDSLDLAEKNLSKDLKSDEQVKGLLMIKKQLEDFLKANGV
                     EAIAAQGEKFNPELHEAAQMEEC"
     gene            complement(517..>1212)
                     /locus_tag="PKK37_05000"
     CDS             complement(517..>1212)
                     /locus_tag="PKK37_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81767.1"
                     /translation="RQKTILDMIIREYVESARPVSSQYLGERYDFGICPASIRIEMQK
                     LTDDGFLTQPHTSAGRIPTDKGYRLFVDQLAEGDGLTKISLDFGAGLEQNDNFDDFFG
                     AMRDLAQSAAAVSEALVLGYMKDAGVFFKEGWEEVLRQPEFGRRDLMDNFLDYLDYLE
                     NNIEKMKPADDFEIFIGQENPFPKAKELAVFSMAFEPAPQREVIFSLVGPKRMAYDKN
                     IALTNEILKMFNL"
BASE COUNT          296 a          324 c          218 g          374 t
ORIGIN      
        1 ggcactcctc catttgcgcc gcttcgtgaa gttcgggatt aaacttctcg ccttgggcgg
       61 caatcgcttc caccccgttg gctttcaaaa aatcttccag ctgtttttta atcatcagca
      121 atccctttac ttgttcatcg cttttcaaat cttttgaaag atttttttcg gccaggtcca
      181 ggctgtccat tatcggcaaa agactgaaga caaacgcctg ccggttatat ttcccgaatt
      241 cctccattct ttggctttct tcttttttgt agttgattaa atccgccttg gcccgtttcc
      301 agccgtttaa attcgcctca gcctgttttt tcacctcatc caattctttt tgcaaaacct
      361 caatttcttt ttccatccaa tttaaatcca aatgtcaaaa tccaaatgtc aaatcaaatc
      421 ctaaatctaa atatcaaatt gaatgcgtat ttttgcgtat tcttttaact ttggattttc
      481 aacttgattt gacatttgag cttcggattt tggattttat aaattaaaca ttttcaagat
      541 ctcgttggtc agcgcgatat ttttatcata ggccatgcgt tttggtccca ccagcgaaaa
      601 aatcacttct ctttgcggcg ccggttcaaa cgccatggaa aacaccgcca gttctttggc
      661 cttaggaaac ggattttcct gcccgatgaa aatttcaaaa tcatcggccg gtttcatttt
      721 ctcaatattg ttttccaaat aatccagata gtccaaaaag ttatccatca aatcgcgccg
      781 gccaaattcg ggctggcgca acacttcttc ccagccttct ttgaaaaaaa cgcccgcgtc
      841 tttcatatag cccagcacca gcgcttcgga aaccgccgcc gcgctttgcg ccagatcgcg
      901 catggcgccg aagaaatcat caaaattatc attttgctcc aatccggcgc caaaatccag
      961 gctgattttg gtcaaaccgt cgccctcggc caattgatcc acgaacaacc gataaccctt
     1021 gtcggtggga atccgccccg cggaagtgtg cggctgagtc aaaaatccgt catcggtgag
     1081 cttttgcatt tcaatgcgga ttgatgccgg acagatgccg aaatcatatc tttcccccag
     1141 atactgcgaa gacaccggcc gcgccgattc cacatattcc ctgattatca tgtccaatat
     1201 cgttttttgc cg
//
LOCUS       DAOQWE010000233         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_152195_length_1211_cov_7.451557, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000233 DAOQWE010000000
VERSION     DAOQWE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152195_length_1211_cov_7.451557"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1211
                     /gene="rny"
                     /locus_tag="PKK37_05005"
     CDS             <1..>1211
                     /gene="rny"
                     /locus_tag="PKK37_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNR81768.1"
                     /translation="KVQDEAQAKAQKMLDDSRQEIARRQKEIAKTETLILKRESNLEQ
                     KIAAFETEESELREKVQKLKSLKENLEELHRRTMEGLERVAGLTKEEALRELMEVLEK
                     EHARNLLEKTRRLESEGIDGFRQRAREILTQAIQKFALSQAQEITTSVVSLPSDDIKG
                     RIIGKEGRNIRALERLTGVEIIVDETPETVVISGFDPTRRQIAKIALEKLIQDSRIQP
                     ARIEETVKIAEEEIAAKIKEAGEQACYDTNVVGLNPRLVQLLGRLKYRTSYGQNALLH
                     SVEVSHLAAAIAAEVGANVKVARKAGLLHDIGKSVDQQIEGTHVEIGVKILEKFNVEP
                     EVVAAVRSHHEDFPYEGVEGMIVQAADQISGARPGARKDTVENYLKRLGELEGIAKSF
                     EGVQKAWALQAG"
BASE COUNT          340 a          264 c          386 g          221 t
ORIGIN      
        1 aaagtgcagg acgaagcgca ggcgaaagcg cagaaaatgc tggatgactc gcgccaggag
       61 attgcgcgcc ggcaaaaaga gattgccaag accgagacgc tgatcttgaa acgggaaagc
      121 aatttggagc aaaaaattgc cgcgtttgaa accgaagaga gcgaattgcg cgaaaaagtg
      181 caaaaactga aatccctgaa agaaaatttg gaggaactgc atcgccggac gatggaaggg
      241 ctggagcgcg ttgcgggatt gaccaaagaa gaagcgcttc gggaattgat ggaagttttg
      301 gaaaaagagc atgcccgtaa tttattggaa aaaacgcgcc gtttggaaag cgaaggcatt
      361 gacgggttca gacagcgcgc ccgggagatt ttgacgcagg cgattcaaaa atttgcgctt
      421 tcacaggcgc aggaaattac cacttcggtg gtgagtttgc ccagcgacga tataaagggc
      481 aggattatcg gcaaggaggg gcgcaatatc cgcgcgctgg agcggctcac gggagtggag
      541 atcattgttg atgaaacgcc cgaaaccgtg gtgatctcgg gatttgatcc cacgcggcgg
      601 cagattgcca agatcgcgct ggaaaaattg attcaggatt ctcgtattca gccggcgcgc
      661 attgaagaaa cggtgaaaat tgccgaagaa gaaatcgccg ccaagataaa ggaagccggc
      721 gaacaggcat gttatgacac gaacgtggtg ggattaaatc cccgcctggt ccagcttttg
      781 ggaagattaa aataccgcac cagttacggg cagaacgcgc tcctgcattc ggttgaggtc
      841 agccatttgg ccgcggcgat tgccgccgag gtgggcgcca atgtcaaagt ggcgcgcaaa
      901 gcgggattgc tccacgacat cggcaaatcc gtggatcagc agattgaagg cacgcacgtg
      961 gaaatcggcg tcaagatttt ggaaaaattc aatgttgagc cggaagtggt ggcggcggtc
     1021 cgatcgcatc acgaagactt tccctatgag ggcgtggaag gaatgatcgt gcaagcggcg
     1081 gatcaaattt ccggcgcccg tcccggcgcc cgcaaagaca cggtggaaaa ttatttgaag
     1141 cggctggggg aattggaagg catcgccaaa tcgtttgaag gagtccaaaa ggcttgggca
     1201 ctgcaggcgg g
//
LOCUS       DAOQWE010000234         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_153150_length_1207_cov_2.054688, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000234 DAOQWE010000000
VERSION     DAOQWE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153150_length_1207_cov_2.054688"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PKK37_05010"
     CDS             complement(<1..1166)
                     /locus_tag="PKK37_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNR81769.1"
                     /translation="MLKPKYFLYARKSTEDDDHQIMSIEAQLFELREYARRENVEILA
                     EFTEAKSAKKPGREKFAEMINEIEKSDGVGILSWHPDRLARNSVDGGKIIYLVDTQKI
                     VSLRFPTFWFEPTPQGLFMLQVAFGQSKYYSDNLVENINRGIRQKLRRGEWLTKAPFG
                     YVNNTKTRTIEPHPTLSKVIVRAFEEFATGKHTLGSLAEFLAELGLETKNRTPLAKAS
                     ISRMLTNQAYLGLVKHKGEYHEGRFEPILSTTLFEAVQEVLRRKAKPRKSKQRHDFPF
                     TGLMTCGECGSAITAQFSRGKCGGIYRYYRCTKKKGVCSQKYIQEKDLAAQIKARLQS
                     VALCDEWTSKMLKQVDEWEKEQNHSSQKFVQNLKNNLFETQEKLDKLVSAYIDG"
BASE COUNT          297 a          276 c          244 g          390 t
ORIGIN      
        1 tcgccgtcga tgtaagcgga aaccaatttg tccagtttct cttgcgtttc aaaaaggtta
       61 tttttcagat tttggacaaa cttttgcgac gagtgatttt gctctttttc ccattcatca
      121 acttgcttta acattttaga agtccactcg tcgcaaaggg caacgctttg aagccgtgcc
      181 ttaatttgcg cggcaaggtc tttttcctga atatattttt gtgagcaaac gcctttcttt
      241 ttcgtgcaac gataatagcg atatattccg ccgcacttgc cgcgcgaaaa ttgcgccgtg
      301 atcgccgagc cgcattcacc gcaagtcatt agtccggtaa atggaaaatc atggcgttgt
      361 ttgctttttc gcggtttcgc tttacggcgc aatacttcct gcacggcttc aaaaagcgtt
      421 gtggaaagaa ttggctcaaa gcgtccttcg tgatattcgc ctttatgctt caccaaacct
      481 aaatacgctt gattggtgag cattcttgaa atggacgctt tggcaagtgg cgttcggttt
      541 tttgtttcca agccaagttc agccaaaaac tccgccaaac ttccgagcgt atgttttccg
      601 gtggcgaatt cctcaaaagc gcgaacaata acttttgaaa gcgtcgggtg cggttctatt
      661 gtgcgagttt tagtattgtt cacataaccg aacggcgctt ttgtcagcca ttcgccacgg
      721 cggagttttt ggcgaatacc gcgattgata ttttccacca aattatctga gtagtatttt
      781 gattgaccga acgccacttg caacataaat aacccttgcg gcgtgggctc aaaccaaaat
      841 gttggaaagc gcaaagaaac tattttttgc gtgtccacga gatagataat ttttccgcca
      901 tcaacgctat ttctcgccaa tctatcggga tgccacgata aaattccaac gccgtcagat
      961 ttttctatct cgtttatcat ttccgcaaat ttttctcgcc cgggtttctt tgcgcttttc
     1021 gcttctgtaa attctgcaag aatttccaca ttttctctac gcgcatattc ccgcaattca
     1081 aataattgag cttcaatact cataatttgg tggtcgtcat cttccgttga cttccgagcg
     1141 tagagaaaat attttggttt gagcataatt ttatgaatta ttattttaga tacgaaaacg
     1201 acaaatc
//
LOCUS       DAOQWE010000235         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_155224_length_1197_cov_5.518389, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000235 DAOQWE010000000
VERSION     DAOQWE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155224_length_1197_cov_5.518389"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..380
                     /locus_tag="PKK37_05015"
     CDS             27..380
                     /locus_tag="PKK37_05015"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNR81770.1"
                     /translation="MELNEFEKLTAAAVEELPQNIKDALKNVAIVVEDGPAKRNLLGL
                     YEGIPENQWGKSDAIRLPDKITIFKSLIEEEARTPEEIKELVRVVVWHEIAHHFGFDE
                     PQTRRLERRWRARKP"
     gene            complement(597..>1197)
                     /locus_tag="PKK37_05020"
     CDS             complement(597..>1197)
                     /locus_tag="PKK37_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNR81771.1"
                     /translation="ERIIRDIPSPRITAGTKGISNLRQIIANDMEREGWHCQCIRCRE
                     VKNEYDAGEKLILTRRDYNASSGTEIFLSFENKEKTKLYSLLRLRLPNGKSKMRANNY
                     SPLRNTEYKLPRQDAAIIREIHTYGIQTPIAGKSVSAQHTGLGKKLIKEAERIAKTEF
                     GAKKIAAISGVGVRQYWRKNGYKLQNTYMVKKLRERIRA"
BASE COUNT          303 a          267 c          310 g          317 t
ORIGIN      
        1 aactctaaaa ctctgatcaa tttgctatgg aattaaatga atttgaaaaa ctgacggccg
       61 cggcggtgga ggaattgccg caaaacatca aagacgcgtt aaaaaacgtg gcgattgttg
      121 ttgaagacgg gccggcgaaa cgcaatttgc tgggattgta tgaaggcatt ccggaaaatc
      181 aatgggggaa gagcgatgcc atccgcctgc cggataagat cacgattttc aaatcattga
      241 ttgaagaaga ggcgcgcacg cccgaagaaa ttaaggagct ggtaagggtt gtggtctggc
      301 acgaaattgc ccatcatttt ggttttgacg agccgcaaac ccgccgcctt gaacgccgct
      361 ggcgcgcccg caaaccttga caaaacccaa aagatattca ttataaagtc aggtgatttg
      421 atggtaatga taatatccgg catcctgccg gaagagagag atgatttgga aaagtatgga
      481 caaatcatcc atgagacaga aaggattgcc cggtttgttc ttcggtccaa gtccaaaagg
      541 cttgggcaaa agcatcagaa aaacaaaatt aagaggcgcc gccggcgcgg cggccgctag
      601 gcgcgaatcc gttcgcgcaa tttttttacc atataggtgt tttgcagttt gtagccgttt
      661 ttgcgccagt attggcgcac gcccacgccg gaaatcgcgg cgattttttt tgcaccgaat
      721 tcggttttgg caattcgctc ggcctctttt atcagttttt tccccaatcc ggtatgctga
      781 gcggaaacgc ttttgccggc aatcggtgtt tggatgccat aagtgtgtat ttcccgaata
      841 atcgccgcat cttgccgggg caatttgtat tctgtgttcc gtaggggtga ataattattc
      901 gcccgcattt tggattttcc gttgggcaac cttaaccgca acaatgaata caattttgtt
      961 ttttctttat tttcaaaact caaaaaaatt tctgttccgc tggaagcgtt gtaatcacgc
     1021 cgcgtcaaaa tcaatttttc gccggcatcg tattcgttct tgacttcgcg gcaacggatg
     1081 cattggcaat gccagccctc gcgttccata tcgttggcga tgatctggcg caaattggaa
     1141 atgcctttgg ttccggcggt gatgcgcggg gagggaatgt cgcggatgat ccgctca
//
LOCUS       DAOQWE010000236         1189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_157017_length_1189_cov_5.500882, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000236 DAOQWE010000000
VERSION     DAOQWE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157017_length_1189_cov_5.500882"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..>1189
                     /locus_tag="PKK37_05025"
     CDS             103..>1189
                     /locus_tag="PKK37_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNR81772.1"
                     /translation="MDNLSENTKRGLRQKLRRGELPGYAPLGYLNDLLKHTMYKDPER
                     FRLVRKLFELYATGNYSLKDLRKLVTSAGLLSRKKNMLSVSNIQSILSNPFYYGVFKY
                     NGEMYDGKHEPMIPKKLFEACQKVMADRSRPKKPSQKEYPFRNMLVCGECGCAITSET
                     QKGHNYYRCTKKRDKKCSQKYIREEVLAEEVANELQKVSLSSAWAEWMLTELNKEEAE
                     AAQSGAVFAQNLKDKIKELEGKLDLLLDAHLDNTISREEYTAKKEKIINEKSELSEKS
                     KDFERNGNHWLEPARQFILAAQQAKIIALQENPVAGRDFLKRIGSNRVLLSQKVAIEP
                     KKTWQILYNLPAEARSAEATNSQHTIWL"
BASE COUNT          391 a          266 c          260 g          272 t
ORIGIN      
        1 accggaaaaa taaaatcgct aaaatccccg acattttggt ttgagccgac cccgcaaggc
       61 aaattcatgt tgaatatcgc ttttgggcaa tcaaaatact ttgtggataa tctttcagag
      121 aataccaagc gagggttgcg tcaaaaattg cgccgaggcg aattgcccgg ctatgctccg
      181 cttggatatt tgaacgactt gctaaagcat acaatgtata aagaccccga aagatttcgc
      241 ctcgtccgca aactttttga actttacgca accggaaatt attctctgaa agatttgcgg
      301 aagttggtaa cttccgccgg attgttgagc cgtaagaaaa atatgctttc cgtctctaat
      361 atccaaagca tactttccaa tccgttttat tacggcgtat tcaaatacaa cggcgaaatg
      421 tatgacggaa agcacgaacc aatgattcca aagaaacttt ttgaagcgtg ccaaaaagtg
      481 atggcggatc gttcgcgccc gaaaaagccg agccaaaaag aatatccatt tagaaatatg
      541 ctcgtttgcg gagagtgcgg ttgcgccata acttccgaaa cgcaaaaagg acacaactat
      601 tatcgttgca ccaaaaagcg ggataaaaaa tgttcgcaaa aatatatccg cgaggaagtt
      661 ttggcggaag aagtcgcaaa cgagcttcaa aaagtttctt tgtcttccgc ttgggcggag
      721 tggatgctca ccgaactaaa caaggaagaa gcggaagccg cccaaagcgg agccgttttc
      781 gctcaaaatc tcaaagacaa aataaaagag cttgagggaa agcttgatct acttttagac
      841 gctcatttgg acaacacaat ttcgcgcgag gaatacaccg ccaaaaaaga aaaaatcatc
      901 aacgaaaaat ccgagctttc cgaaaaatca aaggattttg agcgaaacgg caatcattgg
      961 ctcgaaccgg ccagacaatt cattttagcg gctcaacaag ccaaaattat cgctttgcaa
     1021 gaaaatcccg tcgcgggacg ggattttctc aaaaggattg gctcgaaccg cgttttgctt
     1081 tcgcaaaagg tggcgattga acctaaaaaa acttggcaaa tactatacaa tctgcctgcc
     1141 gaggcgcgaa gcgccgaggc aacaaactcg caacatacaa tttggctcc
//
LOCUS       DAOQWE010000237         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_158615_length_1182_cov_8.161491, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000237 DAOQWE010000000
VERSION     DAOQWE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158615_length_1182_cov_8.161491"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..802)
                     /locus_tag="PKK37_05030"
     CDS             complement(86..802)
                     /locus_tag="PKK37_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNR81773.1"
                     /translation="MPFYLSKKQAAVIGALFVGFLAGGTLLWRQHGAGFLSANLGGAW
                     YGSGWTEIKPRTEALPEREDAGTKNAPAKTEKPAPVVWCDFAAATATPQKSVIINEIA
                     WMGTAVNYSDEWIELKNISGQEISLAGWQLQNKNRKIKVVFTGDETLLPQGLYLLERT
                     DDDSAPQAAADKIYKGALGNQNEALYLFDDNCRLADAAMAPAKWPAGDNLSKKTMFRR
                     ADLGWQTSALAGGTPRAENR"
     gene            complement(852..>1182)
                     /locus_tag="PKK37_05035"
     CDS             complement(852..>1182)
                     /locus_tag="PKK37_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81774.1"
                     /translation="KRDKEANLAILRLSGADLFTAGFYQQEDLPLGERIFLAGALPSG
                     EFFANEGTVRAVSGDKIVTNLYEDAASAGAPVFDIEGNFLGIAQVAESGQVAVISVAK
                     IKKAADL"
BASE COUNT          252 a          367 c          293 g          270 t
ORIGIN      
        1 ccgctcgcga ttatccctcg gattacctcg ggacaagcgc gagggataga cccttgaaat
       61 acaaatatcc agcgagtagt tacccttacc ggttttcggc gcggggcgtg ccgccggcaa
      121 gcgccgatgt ttgccagccc aagtccgccc ggcggaacat tgttttcttg gaaagattgt
      181 cgccggccgg ccatttggcc ggcgccatcg ccgcatcggc caagcggcag ttatcgtcaa
      241 acaaatagag cgcttcgttt tggttgccca aagctccttt atagatttta tcggcggcgg
      301 cttgcggcgc ggaatcgtca tcggtgcgct ccaatagata caatccttgc ggcagtaaag
      361 tttcatcgcc ggtgaaaaca actttgatct tccggttttt gttctgcaat tgccagccgg
      421 caagactgat ttcttggccg gaaatatttt tcaattcaat ccattcgtcg ctgtaattga
      481 ccgccgtccc catccaggca atctcattga tgatcaccga tttttgcggc gtcgccgtcg
      541 cggcggcaaa atcgcaccag accaccggcg ccggtttttc cgttttggcc ggcgcgtttt
      601 tcgtccccgc gtcttcccgc tccggcaaag cttccgtgcg cggcttgatt tccgtccagc
      661 cgctcccgta ccaggcgccg cccaagttcg cgctcaaaaa accggcgccg tgttggcgcc
      721 acaacaatgt tcctccggcc aaaaacccaa cgaacagcgc gccgatcacc gccgcttgtt
      781 ttttggacaa ataaaatggc atattaaaac gcaaatattt gcgcggatgg cctgcttccg
      841 gagagcagct tctacaagtc agcggccttt ttgatcttgg ccactgaaat caccgcaact
      901 tgccccgatt cggccacttg ggcgatcccc aaaaaattgc cctcaatatc aaacaccggc
      961 gcgcccgcgg aagcggcgtc ttcataaagg ttggtgacaa tcttatcgcc ggaaaccgcg
     1021 cgcaccgtcc cttcgttggc gaaaaattcg ccggagggca aggcgccggc caaaaaaatc
     1081 cgctctccca gcggcaaatc ctcctgctga taaaatccgg cggtgaataa atccgcgccg
     1141 cttaaacgca aaattgccaa attcgcctcc ttgtcgcgct tc
//
LOCUS       DAOQWE010000238         1182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_158753_length_1182_cov_2.034605, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000238 DAOQWE010000000
VERSION     DAOQWE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158753_length_1182_cov_2.034605"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..>1182)
                     /locus_tag="PKK37_05040"
     CDS             complement(150..>1182)
                     /locus_tag="PKK37_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HNR81775.1"
                     /translation="TVYTDALRYRYMITKKALKKARILAFWEKHGLKAAIDHSGKAKR
                     TLQLWRSQLLKGNGKLESLSEKSTAPKTKRKRLWADQIKVEIKRLREIHPNLGKDKLY
                     PELLAFCAANGLECPSITTIGRLIGDLGGLRTFPQKINHFGRIVKRKPKKVLRKPKHF
                     KALYPGHMVAFDTVELVIFGKKYYIITCEDIYTRMAFAWATASHASLAAKEFFAKVIK
                     LFPFPIANILTDNGSEFKKHFSQALQELCLSHYHTYPKCPKMNSHLERFNRTIQEEFA
                     NYHTGALLFTDNFNRLLIDWLDWYNTRRVHYAFGNKLSPLQFAMSLKVQELTQMPQEC
                     KSGWTHTTY"
BASE COUNT          277 a          279 c          293 g          333 t
ORIGIN      
        1 gtttgatgga cttgagtttt ccctggcggg aacgcaaacg caaataatgc tctgaataag
       61 agcacatctt ggcggcatcg gccagcgaaa tatagttctg attttcgttt tcctccataa
      121 cttcattata tccacccgcc gataatttat caatacgttg tatgcgtcca cccacttttg
      181 cactcttgag gcatttgagt taattcctgg acctttaacg acatcgcgaa ttgcaacggc
      241 gacagcttgt tgccaaaagc ataatgcact cttcgggtgt tgtaccaatc cagccaatcg
      301 ataagcaagc ggttgaagtt gtcggtaaac agcagggcgc cggtatggta attggcaaac
      361 tcttcctgaa tggtgcggtt gaatctttca agatgcgaat tcatcttggg gcacttggga
      421 taggtatggt aatgcgacag gcaaagctct tgcaatgctt gggagaaatg cttcttgaat
      481 tccgaaccgt tgtcggtcag gatattggcg atgggaaacg gaaaaagctt gatgaccttg
      541 gcaaagaatt ccttggccgc cagcgatgcg tggctggccg tggcccaggc aaacgccatt
      601 cgggtgtaga tgtcttcgca agtgataata taatacttct tgccaaagat gaccaattca
      661 accgtatcaa aggctaccat gtggccggga tacaatgctt tgaaatgctt gggcttccgt
      721 aaaaccttct tgggttttcg tttgacaatc cggccgaaat ggttgatctt ttgcgggaag
      781 gtgcgcaatc cgcccagatc gccgatcagc cgtccgatag tggtaatgct tgggcattcc
      841 aaaccattgg cggcgcagaa tgccagcaat tccggataca gcttgtcctt gcccaaattg
      901 ggatggattt cccgcaagcg cttgatctca accttgattt ggtcggccca gaggcgcttg
      961 cgcttggttt tgggcgccgt gcttttctcg ctcaaggatt ccagtttccc gtttcccttt
     1021 aaaagctggc ttctccatag ttgcaaggtg cgcttggctt tgcccgaatg gtcaatggcc
     1081 gctttaaggc catgcttttc ccagaatgcc agaatacgag ctttcttcaa tgctttttta
     1141 gtgatcatat accgatacct taatgcatca gtatataccg tt
//
LOCUS       DAOQWE010000239         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_159792_length_1177_cov_3.054367, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000239 DAOQWE010000000
VERSION     DAOQWE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159792_length_1177_cov_3.054367"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKK37_05045"
     CDS             <1..878
                     /locus_tag="PKK37_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HNR81776.1"
                     /translation="FHNKEYENIKDISVSHLYNLRDTRQYISHSLTVGKTNPTKVLIG
                     QRMKPDNLGIPGFLRVDTVHQGDLDKEKGVYHINIVDEATQWEIIGCVEKISEEYLLP
                     PLAALLEQFPFLIINFHSDNGSEYINKQVAQMLNKMLIKQTKSRARRHNDNALAEGKN
                     NCSIRKNMGYRFIGKEYAPAINAFYKEFFNPYLNYHRPCGYATITTDKKGKEKKKYDT
                     YQMPYEKLKSLPDAEKYLKPGITFSMLDRIAYAQSDNEFGQKMQDKKVELFNGFKTSK
                     LQFPTIFLSRAPVAI"
BASE COUNT          390 a          265 c          226 g          296 t
ORIGIN      
        1 tattccataa taaagaatac gaaaatatca aagatatttc cgtatcgcat ttgtataatt
       61 tgcgcgatac ccggcaatac atttcgcaca gtttgacggt gggaaaaacc aacccgacca
      121 aggttctgat cggccaaaga atgaaacccg acaaccttgg aattcccggc tttttaaggg
      181 tggataccgt tcatcaaggg gatttggaca aggaaaaggg cgtctaccat ataaacatcg
      241 tcgacgaggc aacccaatgg gaaattatcg gttgcgtgga gaaaatctcg gaagaatatc
      301 ttttgccgcc gcttgccgct ttgttggagc aatttccatt cttgatcatc aatttccatt
      361 ccgacaacgg ctcggaatat atcaataagc aagtggctca aatgttgaat aaaatgctga
      421 tcaaacaaac gaaatcgaga gcgcggcggc ataacgacaa cgcgcttgcc gaaggaaaaa
      481 ataactgctc catcagaaag aatatgggct accgattcat cggcaaagaa tacgccccgg
      541 ccatcaatgc tttttacaag gaatttttca acccgtattt gaattaccac cggccttgcg
      601 gctacgccac aatcactacc gacaaaaaag gcaaagaaaa gaaaaaatac gatacctacc
      661 aaatgccata cgaaaaattg aaatcgctgc ccgatgcgga aaagtattta aaacccggaa
      721 tcactttctc aatgctggat aggatcgcat acgcccaaag cgataatgaa ttcggccaaa
      781 aaatgcaaga taaaaaagtc gagctgttta atggttttaa aacctcaaaa ctccaatttc
      841 ccacgatttt tctaagccgc gccccggttg caatttgagg gtcctgcctt gattggaaaa
      901 tactatgaga ttctatcatt acaattattg caaagtaatg cttttttaca aaaaataaat
      961 tgcgcacttc cttggcatca acacaaccaa aaacaaaaaa acggcatttg atgccgttct
     1021 gctgttactt tcttgctgga gcaggataat gaaccaagtt agaacctctt tccaagaaca
     1081 atcagaccaa tctttgtttg aaatagtatc agcttggtag cgattttcac cgcttctcaa
     1141 gctcttttat gcgctcttgg atacttctaa accgaca
//
LOCUS       DAOQWE010000240         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_160078_length_1176_cov_2.169492, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000240 DAOQWE010000000
VERSION     DAOQWE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160078_length_1176_cov_2.169492"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="PKK37_05050"
     CDS             complement(<1..951)
                     /locus_tag="PKK37_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DA1"
                     /protein_id="HNR81777.1"
                     /translation="MEFDKSAVITKINELTKSDNDKTKIGQVEAAKILFAQDLAKIRK
                     NIAKTEFIDPENEININFEKLKKLLAKFGIIYNHPLFIVDRFPKPYDFFDRNGTSGTQ
                     FDDSDNKAFGLEQGIYLKRQRLNPFTSSLLMAHELIHAVIGLRDSNFLARGLEEGFTQ
                     FFGEFYLGGQIFGYQTALNYEINRRLSYPVESQQWEQYTDWLRQGCIFYQKFGIDGMI
                     ELMKQGRGLMKKADESMLQGRLNELKLPKGKWIKELDSAAETILSFNRNLVYSPLAVY
                     LAQNIKLGESVDRLFLRLDIKAPEGQKALKELNERIFSILI"
BASE COUNT          314 a          240 c          205 g          417 t
ORIGIN      
        1 gattaaaatt gaaaaaattc tttcgtttaa ttccttgagc gctttttgtc cttcgggcgc
       61 cttgatatcc aggcgcaaga ataatctgtc aacagattct cccaatttta tattttgcgc
      121 tagataaacg gcaagcggac tgtaaaccaa attgcggtta aagcttaaga tcgtttcggc
      181 ggccgaatcc aattctttta tccatttgcc ttttggcaat ttcaattcat ttagtcgccc
      241 ttgcaacatt gattcgtctg cttttttcat taatcccctt ccttgtttca tcagttcaat
      301 catcccgtca atcccgaatt tttggtaaaa aatgcatcct tgccgcaacc agtcggtata
      361 ttgttcccat tgttggcttt cgacaggata tgagagtctt ctattgattt cataattcaa
      421 tgcggtttga tagccaaaaa tttgtccgcc taaataaaat tccccgaaaa attgagtgaa
      481 tccctcttcc aatccacggg cgagaaaatt ggaatctctc aatccaatca cggcgtggat
      541 aagttcgtgc gccatcaata aacttgaagt aaacggattc aatcgttggc gctttaaata
      601 tattccttgt tccaagccga aagctttatt gtcggaatcg tcaaattgag ttccgctggt
      661 gccgtttctg tcaaaaaaat catatggttt cgggaagcgg tcaactataa aaagcggatg
      721 attgtaaata atcccgaatt ttgccaataa tttttttaat ttttcaaaat taatgttgat
      781 ttcattttcc ggatcaataa attcagtttt tgcgatgttt tttctaattt ttgccaaatc
      841 ctgcgcgaat aatatttttg cggcttcaac ttgtccgatt tttgttttat cgttgtcgga
      901 ttttgttaat tcgttaattt tggtaattac cgcgctttta tcaaattcca atttcgaaaa
      961 atctttttca aagtaaccgc aaaaatcagt tgcgcttgcc caatttcccc ggcgcatgaa
     1021 tactccggtt ttaaatatat taaatccatt cattttatgg aatattttca ttgctatttg
     1081 gttataagtt ttccgacagt tggataaccc gacagtatct gtttgctgtg ttcgtcgtaa
     1141 aattcggcgg tgacatcata tccggccgaa cagccg
//
LOCUS       DAOQWE010000241         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_160849_length_1172_cov_4.832587, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000241 DAOQWE010000000
VERSION     DAOQWE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160849_length_1172_cov_4.832587"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1172
                     /locus_tag="PKK37_05055"
     CDS             259..>1172
                     /locus_tag="PKK37_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81778.1"
                     /translation="MNDGTRTIYVVGAGGRTGMLFCRELQNAARMIGVAFPAEIENIK
                     SGKAKIVRGKNLPEIFTAELVPPEDFGAAIVRNAPDFLWLAARNPVDKITAFYYRHFA
                     NQQNFPALVLSQNGLSAVADAKKGLQEALGQDADKVAIVRVSLINGVDLNTDGDGTLT
                     IGYKLPIKLGFGTVKTYIGDTDVNSSASLGLEEIFKKAGVGAREFRGKDVLAMENAKL
                     FLNLIGMAAATAQGSGEPTAAVGNTDVGAGWRDKEIFKKEMAALREFVLAVNAVRGGF
                     AGKLGGYPVKLLARLALLPVCLLAPWRG"
BASE COUNT          305 a          231 c          361 g          275 t
ORIGIN      
        1 ccttttttgg tgcgttgccg cgcgggcgga agcgattttc ggcgatgatg tccgcgcggc
       61 ggcggcaaag attgcggaat gtttttcgca acaggattcg caatcgggaa agtgaaaaac
      121 aaccgataat cgataaatga taattgataa gcgatcaata ataaatgatc aatgatcaat
      181 gataaataat aaataataaa taataagtga taaatgatta atgattaatg attaattatc
      241 aattatcaat gataaatgat gaatgatggg acaaggacaa tttatgttgt cggcgccggc
      301 ggccggacgg gaatgctgtt ctgccgggaa ttgcaaaacg cggcgcggat gatcggcgtt
      361 gcgtttccgg cggaaattga gaacatcaaa tccggaaagg caaagattgt tcgcggcaaa
      421 aatctgccgg aaatctttac tgccgaactg gtgccgccgg aagattttgg cgcggcgatt
      481 gttcgcaacg cgccggattt tttgtggctg gcggcgcgca atcccgtgga caaaatcacg
      541 gcgttttatt atcgccattt tgccaatcaa caaaattttc ccgcgttggt gctatcgcaa
      601 aacgggcttt cggcggtggc ggatgcgaag aagggattgc aagaagcgct gggacaagac
      661 gcggacaagg tcgcgattgt ccgcgtgtcg ctgatcaacg gcgttgatct gaataccgat
      721 ggcgatggga cattaaccat cggttataaa ttaccaataa aactggggtt tggcacggta
      781 aaaacttaca taggtgacac cgatgtaaat tcttccgcga gtttggggtt ggaggagatt
      841 tttaaaaagg cgggtgtcgg ggcgcgggaa ttccggggga aagatgtttt ggcgatggag
      901 aatgccaaac tgtttttgaa cctaatcggc atggccgccg ccaccgcgca aggctccggc
      961 gagccaacgg cggcagtggg aaatacggat gttggcgcgg gatggcggga taaagaaatt
     1021 ttcaaaaaag agatggcggc actgcgggaa ttcgtgttgg cagtaaacgc ggttcgcggc
     1081 gggtttgccg gcaaattggg cggctatccg gtgaaattgc tggcgcggtt ggcgttgctt
     1141 ccggtttgcc tgctggcgcc gtggcgcgga at
//
LOCUS       DAOQWE010000242         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_163922_length_1159_cov_3.595109, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000242 DAOQWE010000000
VERSION     DAOQWE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163922_length_1159_cov_3.595109"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PKK37_05060"
     CDS             <1..904
                     /locus_tag="PKK37_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNR81779.1"
                     /translation="STPEETQNPMPAEESQTQTPPEAESNLPAEENVIQESEIQGELS
                     TEAPAEEPQSTGKSVIGKSVQSRDITAYHFGGGSEEILFVGGIHGGYAWNTARLAYQL
                     IDYLTAHPEEIPANAKITIIPVLNPDGLAKVVDKTGMFNAADVSTSADTAAARFNANN
                     VDLNRNFDCEWQAKAVWQNKTVSAGSAAFSEPESAAIRDYAQSHSIAGVVAWYGFGGG
                     VYVSSCDSKMLPESEIIMNLYSRLSGYAPNQDFSAYAISGDMTDWFSKNNVPAIGVLL
                     TAANDTEWDKNWAGSKEIIKHFAQ"
     gene            901..>1159
                     /locus_tag="PKK37_05065"
     CDS             901..>1159
                     /locus_tag="PKK37_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81780.1"
                     /translation="MKILGRKLISLVVAGLVLVGAGLLTLSIFNRPKPLAPKPQEIVS
                     PVMEMASTTIAVVGPIHEIIGYSAQKRPIDSYTFGDGKTLLV"
BASE COUNT          309 a          281 c          312 g          257 t
ORIGIN      
        1 atcaacgccc gaagaaactc aaaatccgat gccggccgaa gaatcgcaaa cccaaacgcc
       61 gccagaagcg gaaagcaact tgccggccga ggaaaatgtt attcaagaaa gcgaaatcca
      121 gggagaatta tcaaccgaag cgccggcgga agagccgcaa agcacgggaa agagcgtcat
      181 cggcaaatcg gtgcagagcc gcgatattac cgcttatcat tttggcggcg ggtcggaaga
      241 aattttgttt gtcggcggta ttcacggcgg ctacgcctgg aacaccgcgc gcctggctta
      301 ccaattgatt gactatttaa cggcccaccc cgaggaaatt cccgcaaacg ccaaaattac
      361 cattattccg gtattaaatc ccgatggtct ggccaaagtg gttgataaaa cgggaatgtt
      421 taatgccgcc gatgtttcaa cttccgcgga taccgccgcg gcaagattca acgccaacaa
      481 tgtggatctg aaccgcaact ttgattgtga atggcaggcc aaggcggttt ggcaaaacaa
      541 aaccgtgagc gccggcagtg cggcgttctc cgaaccggaa agcgcggcga ttcgcgatta
      601 cgcgcaaagc cattctattg caggcgtggt ggcgtggtat ggatttggcg gcggagttta
      661 tgtttcaagc tgtgatagta aaatgctacc cgaaagcgag atcatcatga atctttattc
      721 gcgattgtcc ggttatgcgc caaatcagga ttttagcgcc tacgcgattt ccggcgatat
      781 gacggattgg ttctccaaaa ataatgtgcc cgccatcggc gtgttgttga cggccgccaa
      841 cgataccgaa tgggacaaaa attgggcggg aagtaaagag ataatcaagc atttcgctca
      901 atgaaaattt tagggcgaaa attgatatcg cttgttgttg cgggcctcgt gctggttggc
      961 gcggggcttt tgacgctctc aattttcaac cggcccaagc cgttggcgcc aaaaccgcaa
     1021 gaaatcgttt cgccggtcat ggaaatggca agtacgacca ttgccgtggt cgggccaatt
     1081 catgaaatca tcggttattc ggcacaaaaa cgcccgatcg attcctacac ttttggcgat
     1141 ggtaaaacat tattggtct
//
LOCUS       DAOQWE010000243         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_164578_length_1156_cov_6.181653, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000243 DAOQWE010000000
VERSION     DAOQWE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164578_length_1156_cov_6.181653"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKK37_05070"
     CDS             complement(<1..271)
                     /locus_tag="PKK37_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81781.1"
                     /translation="MKPFSNRQLTAAAGICAILLAAILWVLFENTRIKTESVTPAVKK
                     EIEDEEPLPPKELKDTSAPKYEIIGYSAQKRPIDSYTFGDGKTLLV"
     gene            complement(268..>1156)
                     /locus_tag="PKK37_05075"
     CDS             complement(268..>1156)
                     /locus_tag="PKK37_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNR81782.1"
                     /translation="STPEETQNPMPAEESQTQAPPDVENDLPAEDVVAENQDQEVPQN
                     ETYNSPETLIGKSAGGTEIIANHYGNGSKEILFIGGIHGGYSFNTVLLAEKLANNLFS
                     DSSLIPANIKVTIIQNLNPDGSYVFMNNASRFNSLDDSEKLNIRTEGRFNANNVDLNR
                     NFDCNWQPKAVWQNKPVSAGSAAFSEPESRAVRDYAQSHSIAAVVAWYSSGGGVYSSS
                     CNNGILPETQTITDLYAKASGYPAYKNFESYQISGDMTDWFAKNNVPAISVILNTSED
                     EEWDKNWAGIKAILDHYGK"
BASE COUNT          267 a          263 c          270 g          356 t
ORIGIN      
        1 agaccaataa tgttttacca tcgccaaaag tgtaggaatc gatcgggcgt ttttgcgccg
       61 aataaccgat gatttcatat tttggggcgg aggtatcttt gagctccttt ggcggcaacg
      121 gttcttcgtc ttcaatttct ttctttaccg cgggtgttac cgattctgtt ttgattcggg
      181 tattttcaaa caatacccat aaaatcgcgg ccagcaaaat cgcgcaaatt ccggcggcgg
      241 cagtcaattg gcggttggaa aaaggtttca tttgccgtaa tgatcaagaa tcgccttaat
      301 acccgcccaa tttttatccc attcctcgtc ttcggatgta ttcaaaatta cgctgattgc
      361 cggaacatta tttttggcaa accaatccgt catatcgccg gaaatttgat aggattcaaa
      421 attcttataa gccggataac ccgaggcttt ggcataaaga tcggtgatcg tttgcgtttc
      481 cggaagaatg ccattgttac agcttgacga atatactccg ccgccggaac tataccaagc
      541 aactaccgcg gcgattgaat ggctttgcgc gtaatcgcgg acagctctgc tttccggttc
      601 cgaaaatgcc gcactgccgg cactcacggg tttgttttgc caaacggctt tgggttgcca
      661 attgcaatca aagttgcggt tcaggtccac attgttggcg ttgaagcgtc cttcggttct
      721 tatgttcagt ttttcagaat cgtccaagct gttgaaacgg cttgcgttat tcataaaaac
      781 ataagatccg tccggattca aattttggat tatcgttact ttaatgttgg cgggaatcaa
      841 gcttgaatcc gagaataaat tatttgccaa cttttcggcc aaaagcactg tattaaatga
      901 atacccccca tgaatgcccc caatgaaaag aatttccttg gagccgttgc cgtagtgatt
      961 ggcgattatt tcggttccgc ccgccgattt gccgataagg gtttctggag aattatacgt
     1021 ttcgttttgc ggcacttctt ggtcttggtt ttctgcgaca acatcttcgg ccggcaaatc
     1081 gttttcaacg tctggcggcg cttgggtttg cgattcttcg gccggcatcg gattttgagt
     1141 ttcttcgggc gttgat
//
LOCUS       DAOQWE010000244         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_164768_length_1156_cov_1.898274, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000244 DAOQWE010000000
VERSION     DAOQWE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164768_length_1156_cov_1.898274"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKK37_05080"
     CDS             complement(<1..234)
                     /locus_tag="PKK37_05080"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNR81783.1"
                     /translation="MELTEKQELFLQQNSLAVLATADLENQPRAIFVEINSVNKNELV
                     ITDNEMQLTKNNLLENAKVFVLAYGKNYNYCLKI"
     gene            complement(225..>1156)
                     /locus_tag="PKK37_05085"
     CDS             complement(225..>1156)
                     /locus_tag="PKK37_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNR81784.1"
                     /translation="SKTDAKLLPVFPKALRKYKAAADMKVRDSLKYIGIGKVLEIVKN
                     INADIIHNHIGWRLMPFAPLLKPPIVTTLHGPMHTGYQKLVYGNFPDAPVVSISNSQR
                     RPFKKLNYVGTVYNGIDVGKFAYSDKPGDYLAFLGRMSPEKGPLQAIRIAKKCGLKLV
                     MAAKVDSVDKGYFEKEIAPLIDGKQIVFMGEVGHEGKVKLLKNARALLAPIQWEEPFG
                     LFFIEAMACGTPVLAFRRGSVPEVIKNGVTGFAVKTDAEMITAVKMAGTIKRSACRQW
                     VEENFSSAKMARGYEDIYRKVIAQKKIMNRIWN"
BASE COUNT          281 a          302 c          228 g          345 t
ORIGIN      
        1 aatcttcaag caataattat aatttttgcc ataagccaaa acaaaaacct tggcattctc
       61 caacaaatta ttttttgtca attgcatttc attatcggta ataaccaatt catttttgtt
      121 cacactattt atttcaacaa aaatcgcgcg tggctgattt tccaaatccg cggtcgctaa
      181 taccgctaaa ctattttgtt gcaaaaataa ttcttgtttt tcggttaatt ccatattctg
      241 ttcatgattt ttttttgggc gatcactttg cgatagatat cctcgtagcc gcgagccatt
      301 ttggcgcttg agaaattttc ttccacccat tgccggcaag cacttctttt tatcgtcccg
      361 gccatcttta ccgccgtgat catttccgca tcggttttca cggcaaaacc ggtcacgccg
      421 tttttaatca cctcaggcac ggaaccgcga cggaatgcca gcaccggcgt tccgcaagcc
      481 atcgcttcaa taaagaaaag gccgaacggt tcctcccatt ggattggcgc cagaagcgcg
      541 cgcgcatttt taagaagctt caccttcccc tcgtgaccca cctcacccat aaaaactatt
      601 tgcttgccgt caataagagg cgcgatttct ttttcaaagt aacccttatc gaccgagtca
      661 acttttgccg ccatcaccaa ctttaatccg catttcttgg cgatcctgat tgcttgtagc
      721 gggccctttt ccggcgacat tctgcccaaa aacgcgagat aatctcccgg cttgtcggaa
      781 taagcgaatt tgccgacatc tattccgttg tagacggtgc cgacataatt caatttttta
      841 aaaggccggc gctggctgtt tgaaatcgaa accaccggcg catccgggaa attgccataa
      901 acgagctttt ggtagccggt atgcatcgga ccgtgaagcg ttgtgactat cggcggtttc
      961 agcaagggcg caaacggcat gagcctccag ccgatatggt tatggattat atcggcattt
     1021 atgtttttca ctatttccag gactttgccg atcccgatat atttcaaaga atcccggact
     1081 ttcatatccg ccgccgcctt gtattttctc aacgctttcg gaaacaccgg tagcaatttc
     1141 gcgtcggtct tggaat
//
LOCUS       DAOQWE010000245         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_164834_length_1155_cov_5.031818, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000245 DAOQWE010000000
VERSION     DAOQWE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164834_length_1155_cov_5.031818"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1155)
                     /locus_tag="PKK37_05090"
     CDS             complement(<1..>1155)
                     /locus_tag="PKK37_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81785.1"
                     /translation="AVTQSRMEAISSADAYYPVSDFDALAQVLRDLAAGMCGGTITVG
                     KFIGKEGNWVPAGAGWEFNVGGTAAATKVENGGKTDPVSLDAGTYDVTETPQADYTLV
                     NGVCSISNNGGASLGTFDLANNRVKDIDLGVLDIVSCSFYNAANCQKIYGAEVCYDNG
                     WARKSYTWNFPQFCEAAGSEEYEKHPDCDCVESSSKSCTGPRASTTSYTYNFSYCPAK
                     DDLVNDDDADCESTWTCLDWVNAECVSNDEGGKRKQTQSCTDQYGGAKTNEQIVGDAT
                     CGCSQTETGRACFADGMARVEYSLGGQSYCSGTFTAEESDAACACGYSPWQNSGCAGN
                     GTMNQARTQTTQFDYCTALTQTVADASCALPDPVPGCTDPDATNYNSSAT"
BASE COUNT          265 a          382 c          257 g          251 t
ORIGIN      
        1 tgcgtggccg aagaattgta gttggtagcg tcgggatcgg tgcaacccgg aaccggatcc
       61 ggcaaagcgc aagaagcatc ggcaacggtt tgcgtgagcg ccgtgcaata atcaaattgc
      121 gtggtttgcg tccgcgcttg attcatcgtg ccattgcccg cacagccgct gttttgccac
      181 ggcgaataac cgcaagcgca agccgcatcg ctttcctcgg cggtgaaagt tcccgaacaa
      241 taggattgcc cgccgagaga atattccacc cgcgccatcc cgtcggcaaa acaagcccgg
      301 ccggtttcgg tttgcgaaca cccgcaagtt gcatcgccga caatctgctc gttggttttt
      361 gcgccgccat attgatcggt gcaagattgc gtctgcttgc gcttgccccc ctcatcgttg
      421 gaaacacatt cggcattgac ccaatccaag caagtccaag ttgactcgca atccgcgtca
      481 tcatcattga ccaaatcatc cttggccgga caataggaga aattgtaagt gtaggaagtt
      541 gttgaagccc gcggcccggt gcaactcttg cttgaagatt ccacacagtc gcaatccgga
      601 tgcttttcat attcctcgct ccccgccgcc tcgcaaaact gcggaaaatt ccaagtgtag
      661 ctctttctgg cccagccatt atcatagcaa acttccgccc cgtaaatttt ctggcaattc
      721 gccgcattgt agaacgaaca agaaacaatg tccaaaacgc ccagatcaat atccttgacc
      781 cggttgttgg ccaaatcaaa agtccccaaa ctcgccccgc cattgttgga aattgaacac
      841 accccgttaa ccaaagtgta atccgcctgc ggcgtttccg tcacatcata ggttcccgcg
      901 tccagcgaca ccggatccgt tttgccgccg ttttccacct tggtcgccgc cgccgtgcca
      961 cccacattaa actcccagcc cgcacccgcc ggcacccaat tcccctcctt gccaataaac
     1021 tttcccaccg taatcgtccc cccgcacatc cccgccgcca aatcccgcaa aacctgcgcc
     1081 agcgcatcaa aatcactcac cggatagtag gcatccgccg aagaaatcgc ctccattctg
     1141 ctttgcgtca ccgcc
//
LOCUS       DAOQWE010000246         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_168024_length_1143_cov_1.766544, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000246 DAOQWE010000000
VERSION     DAOQWE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168024_length_1143_cov_1.766544"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PKK37_05095"
     CDS             <1..1082
                     /locus_tag="PKK37_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81786.1"
                     /translation="GNAAVAYGLPCHTNSNQAIANISLSDLSPFYLSIFLNSFYGKQQ
                     TARLTISSVQPNLLLTQVKEIKVFIPRKDFQEIIEKIYKKAYQNKNNGKALYAETERL
                     LLDELGVLNWNPGVVLSYVKNFSETQKAERFDAEYFQPKYEEIAAAVKKYPGGFDELG
                     NLAKIKKSVEPGSEAYRESGVPFVRVSNISKFELSDNNQQFISETLYEELKKHQPCVD
                     EILLSKDATPGMAYYLNETPQKMIVSGGILRLKVASPRILPEYLTLVLNSAIVQKQIE
                     RDSGGSIINHWRPDQVKATLIPLLGDEKQNEIKQLVAESFKCRKLSKALLEIAKRGVE
                     IAIETDEVAAQTWITVELEKLKIKL"
BASE COUNT          366 a          194 c          270 g          313 t
ORIGIN      
        1 tagggaatgc cgctgttgct tatggtttgc cttgccatac gaattccaac caagcgattg
       61 cgaatatatc gctcagcgat ttatcgccat tttatttgtc tatattttta aattcttttt
      121 acggaaagca gcaaacggcg agattgacca ttagcagtgt tcaaccgaat ttacttttaa
      181 cgcaagtaaa agaaatcaaa gttttcatac cacgaaaaga ttttcaagag attattgaaa
      241 agatatataa aaaagcttat caaaataaga ataatggcaa ggcattatat gcggaaacgg
      301 aacggttgct tttggacgaa cttggggttt tgaattggaa tccgggagtt gttttgagct
      361 atgtgaaaaa tttttctgag acgcaaaagg cggaacggtt tgatgccgag tattttcagc
      421 cgaaatatga ggaaattgcc gcggcggtga aaaaatatcc gggtgggttt gatgaattgg
      481 ggaatttggc gaagatcaag aaaagcgttg aaccggggag tgaggcctat cgcgaaagcg
      541 gcgtgccgtt tgtgcgcgtg agcaatattt caaagtttga actttccgat aacaaccagc
      601 aattcatttc cgaaactttg tatgaagaac tgaaaaaaca tcagccctgc gttgatgaaa
      661 ttttgctgtc aaaagacgca acgcccggca tggcttatta tttaaacgaa acgccgcaaa
      721 aaatgatagt ttcgggcggt attttgagat tgaaggttgc cagcccgcga attttacccg
      781 aatatttaac gctggttttg aattctgcga ttgttcaaaa acaaattgag cgcgattcgg
      841 gcggctccat tataaatcat tggcggccgg atcaagtgaa agccacgctc attccgcttt
      901 tgggagatga aaaacaaaat gaaataaaac aacttgtcgc cgaatcgttt aaatgccgca
      961 aactttccaa agcgttgctg gaaattgcca aacgcggagt ggagattgcg atagaaacag
     1021 atgaagttgc ggcgcaaact tggattaccg tggaattgga gaaattaaaa atcaaattgt
     1081 aaatttacca tggaacaggt attaaaactt agcattgatt ttggaatgat aattgttaag
     1141 acg
//
LOCUS       DAOQWE010000247         1141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_168385_length_1141_cov_2.953959, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000247 DAOQWE010000000
VERSION     DAOQWE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168385_length_1141_cov_2.953959"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PKK37_05100"
     CDS             complement(<1..1122)
                     /locus_tag="PKK37_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81787.1"
                     /translation="MSIGKCQQFLRFIFGLMIFLLLFSGGLSRPLSAHAAGGFGGIEI
                     AADFNFSPVIAAADGIFSEIFRKAGAGITAVADFCVAAGETFGREIAATGRKIAGGAQ
                     SFSATAGATAGRGAASLKSGISEIKNDLSQGAAAVKDEVFPEFCRRTVAIAADAAGFT
                     VALGEKSEQKITAGVKTAATAGNSAIENAFAGIAIVGDMGQVAGSVAASGIFAANNAG
                     QRAGRAAMAGVFAVGSAGQGAAQTAAAGISAAGNGFSFATDTAAGAIAAGVYNLGDAF
                     GQTLGLAGDILELGRGNVAYGWRVADYDLKIAARAGQASVAAFFDSVKNGTRDRLRRW
                     LGIEDSPGNDQLSMINDSNNQEFSEGLTLLEDQRVRPSEN"
BASE COUNT          245 a          363 c          303 g          230 t
ORIGIN      
        1 attttcagag ggtctaaccc tctgatcttc caagagggtt agaccctctg aaaattcttg
       61 attattggaa tcattgatca ttgataattg atcattgccc ggagaatctt ctattcccag
      121 ccagcggcgc aagcggtcac gggtaccgtt cttgacactg tcaaaaaacg ccgccacact
      181 cgcctgtccg gcgcgcgcgg cgattttgag atcgtaatcg gcaacccgcc atccgtaggc
      241 aacgtttccc cggccaagtt ccaaaatgtc tccggccaat cccagcgtct ggccaaaagc
      301 atctcccaaa ttgtagactc cggccgcgat cgcgccggcg gcggtgtcgg tagcaaacga
      361 aaatccgttc cccgcggccg aaattccggc ggcggccgtc tgcgccgccc cttgccccgc
      421 actgccgacg gcaaaaaccc cagccatagc ggcgcgtccg gcgcgctgcc ccgcgttatt
      481 ggcggcaaaa atcccggacg cggcgacgct ccccgcgact tgccccatat cgccaacgat
      541 ggcaattccc gcaaaggcgt tttctatggc agaattgccg gcggtcgccg ccgttttcac
      601 gccggcggta attttttgct cggatttttc tcccaacgcc acggtaaatc ccgccgcgtc
      661 cgcggcaatg gcaaccgtcc ggcggcaaaa ttcagggaaa acctcgtctt ttaccgccgc
      721 ggcaccttgc gaaagatcgt tttttatttc ggagattccg gatttcaggc tcgccgcgcc
      781 gcgtccggca gtcgcgccgg cggttgccga aaatgactgc gcgccgccgg caattttgcg
      841 gccggtggcg gcaatttccc ggccgaaggt ttctcccgcc gcaacgcaaa aatcggcgac
      901 agcagtgatg ccggcgccgg ctttccggaa aatttcggag aaaattccgt ccgcggcggc
      961 aatgaccggg gaaaaattaa aatcggcggc aatttcaatg ccgccgaaac cgccggcggc
     1021 atgcgccgaa agcggccggc tcaaacctcc cgaaaacagc aggaggaaaa tcatcaggcc
     1081 gaagataaat cgcaaaaatt gttggcattt tccaatgctc atcgtttctc tttaaagcgc
     1141 t
//
LOCUS       DAOQWE010000248         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_169665_length_1136_cov_2.857539, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000248 DAOQWE010000000
VERSION     DAOQWE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169665_length_1136_cov_2.857539"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..132
                     /locus_tag="PKK37_05105"
     CDS             22..132
                     /locus_tag="PKK37_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81788.1"
                     /translation="MSTIGKNIKKLRQEKGVSQDKLSKLADISLNTVVKN"
     gene            119..223
                     /locus_tag="PKK37_05110"
     CDS             119..223
                     /locus_tag="PKK37_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR81789.1"
                     /translation="MLKIELDQSPNPTLETIQKLAKALGVSLDDLVNK"
     gene            228..>1136
                     /locus_tag="PKK37_05115"
     CDS             228..>1136
                     /locus_tag="PKK37_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNR81790.1"
                     /translation="MQKEAKARIKINGLLQNAGWRFFDDENGKANIVLENNVKITKQT
                     IDEFGNDFEKTKNGFIDFLLLDDKGFPLVVLEAKSEDKDPLDGKEQARRYAQSQNARF
                     IILSNGNLHYFWDLERGNPTVITAFPTFESLKHLETFKPSFSTLSEEKVESDYIVQTQ
                     NPNYQKDPRWADETQRADFIKEQDLKFLRPYQLAAIHALQNSAKKGNDRYLFEMATGT
                     GKTLVSAAVIKLFLRTGNAKRILFLVDRLELEDQAHKNFKKILHNDFTSVIYKENRDD
                     WRKAEIVVSTVQSLSFDNKYKRLFSPT"
BASE COUNT          401 a          231 c          210 g          294 t
ORIGIN      
        1 taatacatcc cgcaaacaat tatgtcaaca atcggtaaaa atataaaaaa gttaagacag
       61 gaaaagggtg tttcgcagga taagctctca aaactggcgg atatttcact taatacagtt
      121 gttaaaaatt gagcttgacc aaagcccaaa tcccaccctt gaaacgatcc aaaagctcgc
      181 caaagctctt ggagtttcgc ttgatgattt agtcaacaaa taaaattatg caaaaagaag
      241 ccaaagccag aataaaaatc aatggattgc ttcaaaatgc cggttggaga ttcttcgacg
      301 acgaaaatgg aaaggcaaac attgttttag aaaataatgt aaaaatcaca aagcaaacca
      361 tagatgaatt tggcaacgat tttgaaaaaa ccaaaaatgg atttattgat tttttattgc
      421 ttgacgacaa aggatttccg cttgtagttt tagaggcaaa atcggaagac aaagacccgc
      481 ttgacggcaa agagcaagcc cgccgttacg cccaatctca aaacgctcgc tttatcatac
      541 tttcaaacgg caaccttcat tatttttggg atttggaaag aggcaaccct accgttatta
      601 ccgcctttcc gacttttgaa tccttgaagc atttggaaac tttcaaaccg agcttttcga
      661 ctctttcaga ggaaaaagtt gaaagcgact atattgtcca aacgcaaaac ccaaactatc
      721 agaaagaccc ccgctgggca gacgaaacgc aaagggcgga ttttatcaaa gagcaagatt
      781 tgaaattttt gcgaccctac caacttgccg caattcatgc tttgcaaaat tccgctaaga
      841 aaggaaatga ccgttatctt tttgaaatgg caaccggaac aggtaaaaca ctcgtttccg
      901 ctgctgttat caagttattt ttgcgaacag gcaacgccaa aagaatttta tttttagttg
      961 accgcttaga gctggaagac caagcacaca aaaacttcaa aaaaatcctg cataatgatt
     1021 ttactagtgt gatttacaaa gaaaaccgcg atgactggcg aaaggcggaa attgtcgttt
     1081 ctaccgttca aagtttatcg tttgacaata aatataaaag acttttttcg cccacc
//
LOCUS       DAOQWE010000249         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_169807_length_1136_cov_1.816836, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000249 DAOQWE010000000
VERSION     DAOQWE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_169807_length_1136_cov_1.816836"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..212)
                     /locus_tag="PKK37_05120"
     CDS             complement(69..212)
                     /locus_tag="PKK37_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81791.1"
                     /translation="MVKVSIKTFTGVRAKERKEMLAKLGYDLRKIKGVNEICAVKKNG
                     KIV"
     gene            complement(409..>1136)
                     /locus_tag="PKK37_05125"
     CDS             complement(409..>1136)
                     /locus_tag="PKK37_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81792.1"
                     /translation="VLALDETGKASFNHLSKNFVLSGLILPENFKPEFDKSARKLKKK
                     FFNDEEIVFHCRDMLRKKGPFAILEDKNTEIKFWSEFVVLLNSDKLAPAFVIVDKSKA
                     KKLGWNDIAILRRSYNKILEEFVKKHLGGNNGKIIAESDSHQDKYLIEAHNRLQSTGL
                     PSEGIAGADYRNKITSLSLVNKFNADIDIQTADSLATMADTVYAMKMGGCKKPTKVQT
                     IMKRLINRKITDKNNPGIFEILA"
BASE COUNT          296 a          257 c          181 g          402 t
ORIGIN      
        1 ttttattccc ggcgatatcc gccgcaaacg gttttccggg atcccccgat tcgtcaataa
       61 aaatataatt aaacgatttt gccatttttt ttcaccgcgc aaatttcatt gacacccttg
      121 attttcctca aatcataacc aagcttcgcc agcatctcct tgcgctcctt tgctctaact
      181 ccggtaaatg tttttattga tactttcacc ataaaatttc ttgatttttt aataaaattc
      241 cgacctttct cccttacttc cattataaag attatttcag aggtagtaaa aatttttaag
      301 gttatttttc aacaaatcaa ctcccttgac aaaccgttgt gaaaagctat gattggggta
      361 taaaaagtcg gtagatgtgc ggatagccat ggccgatttt tttgtatttc aagcgagtat
      421 ttcaaaaatt ccgggattgt ttttatcggt tatttttctg tttatcaacc gcttcattat
      481 tgtttgcacc ttggtcggct ttttacagcc gcccatcttc atggcataga cagtatccgc
      541 cattgttgcc agactgtctg ccgtttggat atcaatatcg gcgttgaatt tattcaccaa
      601 actcaaagat gtgattttat ttctataatc cgcgcccgct attccttcgg acggcagtcc
      661 cgttgattgc aatctgttgt gcgcctcaat caaatattta tcttgatgcg aatccgattc
      721 cgcgatgatt ttcccgttat ttccgccaag atgtttttta acgaattctt ccaaaatctt
      781 gttatagctg cgccgcaata tcgcaatgtc gttccagccg agtttttttg ctttgctttt
      841 atcaacaata acgaaagccg gcgccaattt atcggaattc agcaaaacga caaattccga
      901 ccaaaatttg atttccgtgt ttttgtcttc caaaatcgca aacggaccct ttttccgtag
      961 catatcccgg caatgaaata ctatttcttc gtcattgaaa aattttttct tcagctttct
     1021 tgccgattta tcaaattcgg gtttaaaatt ttcgggcaag atcaaacccg ataaaacaaa
     1081 attttttgag agatgattaa aacttgcctt ccctgtttca tccaatgcca acactt
//
LOCUS       DAOQWE010000250         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_170162_length_1134_cov_3.665431, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000250 DAOQWE010000000
VERSION     DAOQWE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170162_length_1134_cov_3.665431"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKK37_05130"
     CDS             complement(<1..753)
                     /locus_tag="PKK37_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HNR81793.1"
                     /translation="MKTTISVIKADLGAIGGHIKPSRALLDAVENHIKSKSKGLAIDW
                     RITFTGDDIAILLTHNKGVENEKIHRLCWDAFIAGMKVAKEQGLYGAGQDLLKLEFKG
                     IVKGLGPAVAEMEFEERDAESFLMIAADKTDPGAFNLPLYLSFCDPMYNSGLLISPKI
                     RQGYVFRVIDMLPSDGERSIELKSPEEIYDLAVLLRDQERFAIASVVSRAAGEPAAAA
                     STTRLHNIAGKYAGKDDPVMIVRTQKDFPGSGE"
     gene            complement(936..>1134)
                     /locus_tag="PKK37_05135"
     CDS             complement(936..>1134)
                     /locus_tag="PKK37_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81794.1"
                     /translation="AGDAFGSGFMSGMLASGNDIEYSMRLALINAKHCLLSRIPEGLP
                     AGRTLRLIEQENIKIKIRPLG"
BASE COUNT          281 a          339 c          240 g          274 t
ORIGIN      
        1 ctctcccgaa ccgggaaaat ctttctgcgt tctgacgatc atcaccggat cgtccttacc
       61 cgcgtattta cccgcgatat tgtgcaggcg cgtggtgctg gccgccgccg ccggctctcc
      121 cgcggcgcga gaaaccaccg acgcaatcgc aaatctttcc tgatcgcgca aaagcaccgc
      181 caaatcatag atttcttcgg gagatttcaa ttcaattgat ctctcgccgt cggacggcaa
      241 catgtcaatc acgcgaaaaa cataaccttg ccggattttc ggcgagatca gcagtccgga
      301 attatacatc ggatcgcaaa acgatagata aagcggcaaa ttaaatgccc ccggatcggt
      361 cttatcggcg gcaatcatca aaaacgactc cgcatcacgc tcttcaaatt ccatttccgc
      421 cacggccggc cccaacccct tcactatgcc cttgaattca agcttcaaca aatcctgccc
      481 cgcgccgtaa agcccctgtt ctttggcgac tttcatcccc gcaataaacg cgtcccagca
      541 caagcggtgg attttttcat tttcaacgcc tttgttgtgc gtcaacaaaa tcgcgatatc
      601 gtcgccggta aaagtgatgc gccagtcaat cgctagtccc ttgctctttg attttatgtg
      661 attttccacc gcatccaaca acgcccgcga tggtttgata tggccgccga tcgcccccag
      721 atccgccttg ataacactga ttgtcgtctt cataattttt ttggcttttt atgattacat
      781 tataaagcaa atcgcgcaaa aaacgcaagc cgcacgcgac ataaccactc atcgggttta
      841 gaattctttt gccggagtct aaccgctcgc gactatccct cgggattgcc tcgaggcaag
      901 cgtgagaaac agatcattaa ttttggaaaa cgccatcaac ccaacggacg gattttaatt
      961 ttaatgtttt cttgttcaat caaacggaga gtccggccgg cgggcaagcc ctcggggatg
     1021 cgcgacaaca agcaatgctt ggcattgatc agcgccagcc tcattgaata ttcaatatcg
     1081 ttgcccgacg ccagcattcc cgacataaac cccgaaccga aagcgtctcc cgcg
//
LOCUS       DAOQWE010000251         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_171781_length_1128_cov_1.853681, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000251 DAOQWE010000000
VERSION     DAOQWE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171781_length_1128_cov_1.853681"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..>1128
                     /locus_tag="PKK37_05140"
     CDS             106..>1128
                     /locus_tag="PKK37_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNR81795.1"
                     /translation="MKTILKTGITVKDICAGFVYNELEGKGLFGLSGKLTIQPEYQRN
                     YIYASNGGKKEAAVIESILKGYPLGLIYFNKVSDDNLEVLDGQQRITSVGRFVNDKFA
                     IKDGNGMEQYFSGMAADKKAKIRETKLLIYECEGRESEIKEWFKTINIAGVPLNEQEL
                     LNAVYSGPFVTLGKAEFSNSQNANIQKWSAYIKGSANRQDFWACALDWVSNGNVGDYM
                     SCHRFDKNIAGLKTYFNSAIDWISGVFIDVESEMCGLEWGRLYEQYHREAYDPKKVSA
                     KLKRLYGDPYVKNRRGIFEFILGGEKDTKLLDVRVFDEAIKKTTYAAQTAIAESKEKS
                     NCPLCAI"
BASE COUNT          353 a          195 c          314 g          266 t
ORIGIN      
        1 tggcgggtag cgccagaaag gatattgaaa aaagaactgg ccgcaaagtt attaccggag
       61 agaatgccgg acatttcaaa aaaataaacg acaaaaacca ataatatgaa aacgatttta
      121 aaaaccggca ttactgttaa agatatttgc gcggggtttg tttacaacga gctggaaggc
      181 aagggattgt tcgggttgtc cggaaaatta accattcagc cggagtacca gcgcaactat
      241 atctatgctt ccaatggcgg caaaaaagaa gcggcggtta ttgaatcaat cctcaagggc
      301 tatccgctgg ggttgattta tttcaacaaa gtgagcgatg ataatttgga ggttttggac
      361 ggacaacagc gcatcaccag cgtggggcgg tttgtcaatg acaaatttgc gattaaagat
      421 ggaaacggca tggagcaata ttttagcggg atggccgccg acaagaaagc aaaaatccgg
      481 gaaacaaaac ttttgattta tgaatgtgaa ggcagggaaa gcgagattaa agaatggttt
      541 aaaacgatca atattgccgg ggtgccgtta aacgaacagg aattgctaaa cgcggtttat
      601 tccgggccgt ttgttacgct gggcaaggcg gagtttagca acagccaaaa tgccaatatc
      661 cagaaatgga gcgcttatat aaaaggcagt gcgaatcggc aggatttttg ggcgtgcgcg
      721 cttgattggg taagcaatgg gaatgtcggc gattatatga gctgtcatcg ctttgacaag
      781 aatattgccg gactgaaaac atattttaac agcgcgattg attggatttc cggcgtgttt
      841 attgatgtgg aaagcgaaat gtgcgggttg gagtgggggc ggttgtacga gcaataccat
      901 agagaggcat atgatccaaa aaaggtgtcc gcaaaattaa aaaggctcta tggtgatccg
      961 tatgtgaaaa accgccgggg catttttgag tttatccttg gcggagagaa ggacacaaaa
     1021 ttgttggatg tccgggtgtt tgatgaagcg attaaaaaaa cgacttacgc ggcgcagacg
     1081 gcgattgccg aatccaagga aaaatcaaac tgcccgcttt gcgcgatt
//
LOCUS       DAOQWE010000252         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_171854_length_1127_cov_6.889925, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000252 DAOQWE010000000
VERSION     DAOQWE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_171854_length_1127_cov_6.889925"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1039)
                     /locus_tag="PKK37_05145"
     CDS             complement(188..1039)
                     /locus_tag="PKK37_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81796.1"
                     /translation="MNKELVDYIKQQSSDGVSKSKVTEVLLEQGWHQAEIDEAFADAQ
                     GVAGGMVQTESSAAVAAGTGGKKNLLIAAVAFLILVLVFAVVVSFMGKGEKEEVSSLP
                     ESQEAPAAEGLQGETATEGEDQIDPAIIAEISQLEKSITPPAGWIVRQGMVNYRPMAI
                     FFKPEAEKDGNGEKIINEYVNVIRDNLLNNANDYVEKAKTAWQAKNVDYSLVSERKVN
                     LSDGAGAVLLTGSLVQNDAEIKNMQLFVGKGDVMYIISGFVLAKNWDAEKDMIGAAVM
                     SFKFPEY"
BASE COUNT          295 a          326 c          222 g          284 t
ORIGIN      
        1 gcggacatca tcgccgaaaa tcgcttccgc ccgcgcggca acgcaccaaa aaaggaacgc
       61 aacggcaaaa accagcgccg ccagccacgc ggaaaatttc gccaggtccg acaaatcgtt
      121 tttgttgggc ataccgcaaa atggcaaaag ctcccgcaaa acgggaactt tgccaaaatc
      181 tcaaaaatca atattccgga aatttaaacg acatcaccgc cgcgccaatc atatcttttt
      241 cggcgtccca atttttcgcc aaaacaaaac cgctaatgat atacatcaca tccccctttc
      301 caacaaagag ttgcatattc ttgatctccg catcgttttg gaccaaagac ccggtaagca
      361 acaccgctcc ggcgccgtcg gaaagattaa ccttgcgctc gctaaccaag ctataatcaa
      421 cattcttggc ctgccaagca gttttcgcct tttcaacata atcattggcg ttattgagta
      481 aattgtcgcg gataacattc acgtattcgt tgatgatttt ttcaccgttc ccgtcttttt
      541 ccgcttccgg cttgaaaaat atcgccatcg gacggtaatt caccatgccc tggcggacaa
      601 tccagcccgc cggcggcgta atgctttttt ccagttggga aatttcggcg atgatcgccg
      661 gatcaatttg atcttcgccc tcggtagcgg tttcaccttg caaaccttcc gccgccggag
      721 cttcctggct ttccggcaaa gacgaaacct cttccttctc accctttccc ataaacgaaa
      781 cgaccacggc gaaaaccaaa accaggatca agaatgcaac ggccgcgatc agcagatttt
      841 ttttgccgcc ggtaccggcg gcaacggcgg cggagctttc cgtttggacc attccgccgg
      901 caacgccttg ggcatccgca aacgcttcgt caatctccgc ttgatgccag ccttgttcaa
      961 gcaaaacctc cgtaactttg ctttttgaaa cgccgtccga cgattgctgt tttatgtagt
     1021 caacaagttc tttgttcata aaaatttgcg ggaattttta atttgctttt gaaaaaatac
     1081 attcccatta tttcattttc gcaaacaaaa aaacgcttgt caaaccc
//
LOCUS       DAOQWE010000253         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_175403_length_1114_cov_2.226629, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000253 DAOQWE010000000
VERSION     DAOQWE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175403_length_1114_cov_2.226629"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKK37_05150"
     CDS             complement(<1..196)
                     /locus_tag="PKK37_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81797.1"
                     /translation="MDLVYVVGKGGLWGHNELKFSIRSAEKFLKFDRLFLVGYKPTFI
                     NSKANHILIEDNQGHKYKNVT"
     gene            complement(201..>1114)
                     /locus_tag="PKK37_05155"
     CDS             complement(201..>1114)
                     /locus_tag="PKK37_05155"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HNR81798.1"
                     /translation="QTPQDYTANLHGKKALRLKLQLKEQFAKEIKKMRQKVKKSLTKE
                     VKDNSLFKNLDKRKEYWEFVVKNIDNKSKRVETLMNKIAAEQMDAFVKKLGKKKPKNK
                     SEVAEMFDIEKQIKEFAKAILPLIVSIFEEAQDDALDLVGEKKITSGIEKKLSPKVMG
                     LLKARAAFFAESVNNTTLDKLALTLSEGIDNGESVPKLKDRVSEVYDEFPKYRANTIA
                     RTETNAVVNEANLNVYDEVKYIEGKEWIATLDDRVRDSHLLLDGEIVAKDKKFSNGLR
                     YPGEAGGNADETINCRCAIAPVWNLKE"
BASE COUNT          254 a          279 c          195 g          386 t
ORIGIN      
        1 gggtaacatt cttgtattta tgtccttgat tatcctcaat caatatatga ttcgccttgc
       61 tatttataaa agttggctta taccccacga ggaataacct gtcaaatttc aagaactttt
      121 ccgcactccg aattgaaaac tttaattcgt tatgccccca aagtccgcct ttgccgacaa
      181 catataccaa atccattgtt ttattctttt aagttccaca cgggagcgat ggcgcaccga
      241 caatttattg tttcatcggc gttgcctccc gcctcgcccg gatacctcaa tccattggaa
      301 aacttcttat ccttggcgac aatctcgccg tccaacaaaa gatggctgtc cctcaccctg
      361 tcgtccaagg tggcaatcca ttccttgccc tcaatgtatt ttacttcgtc ataaacatta
      421 agattggctt cattgacaac ggcgttggtt tccgttctcg caatggtatt tgccctgtat
      481 ttcggaaatt cgtcataaac ttccgacacc ctgtctttta attttggaac gctttcgcca
      541 ttgtcaatcc cctcgcttaa agttaacgcc aacttgtcca aagtggtgtt attgacgctc
      601 tcggcgaaaa aggcggctct ggctttcaaa agccccataa ccttgggcga taattttttc
      661 tcaatcccgc tggttatttt cttttcgccc accaaatcca aagcgtcgtc ttgggcttcc
      721 tcaaaaatgg acacgataag aggcagaatt gctttggcaa attccttgat ttgcttttca
      781 atgtcaaaca tttcggcgac ctcgcttttg tttttgggtt tctttttccc aagtttcttg
      841 acaaaagcgt ccatctgttc tgcggcgatt ttattcatca gggtttccac ccgcttactt
      901 ttattgtcaa tgtttttgac aacaaattcc cagtattcct tgcgcttatc aaggttcttg
      961 aaaaggctgt tatccttgac ttccttcgtc aaagattttt taaccttttg ccgcattttt
     1021 ttaatctctt tcgcaaactg ctctttcaat tgtaatttca atcttaacgc ttttttgccg
     1081 tggaggtttg cggtataatc ctgcggagtt tgcg
//
LOCUS       DAOQWE010000254         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_176093_length_1111_cov_5.931818, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000254 DAOQWE010000000
VERSION     DAOQWE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176093_length_1111_cov_5.931818"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..621)
                     /locus_tag="PKK37_05160"
     CDS             complement(241..621)
                     /locus_tag="PKK37_05160"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNR81799.1"
                     /translation="MVKKNIFSVAALYRKIGGLAVFQLLFFCPQVLLADNDVPYVEVP
                     TNVSITGMLGKIRGYFFGAVIAACVFMVLWGGFDLATSGGDETKVKNARNRIMYASIG
                     LIVAALSMGIVSLIMSVAQVGGGE"
     gene            complement(742..>1111)
                     /locus_tag="PKK37_05165"
     CDS             complement(742..>1111)
                     /locus_tag="PKK37_05165"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81800.1"
                     /translation="AAAMFSLLCLPAGIARADETADAPAADNQGGGSGEAVLVSPLQG
                     NLTVEDIVGKIAGVIFGLALMICPILIIWGGFEIATASGDQNKITKGKQIITFSLVGL
                     AIIALSAAFVEAIKQILGAD"
BASE COUNT          280 a          327 c          233 g          271 t
ORIGIN      
        1 gcccttcggc cgctttagaa tctgcctttg gcataaattc atttggagtt gtttgatttg
       61 gagttatttg acccaaaacc aaaaatggcg caaacattaa cgaaaccaat gccagcgaaa
      121 cgattttttt tgctttgttg accatattcg ttgttttatt attttattgt tttaaatttt
      181 aatgattaca actcccggct ccattatatc ccaaccgcgt ccccggcgtc aaatcaaaac
      241 tcattctccc ccgccgactt gggcaacgct cattatcaaa ctcacgatcc ccattgacag
      301 cgccgccact atcaagccaa tggaagcata catgatgcgg ttcctggcat ttttaacctt
      361 ggtttcgtcg ccgccggaag ttgccagatc aaatccgccc caaaggacca taaacacgca
      421 ggcggcgatc accgcgccga aaaaataacc gcggattttc cccagcattc cggtgatgct
      481 cacgttcgtg ggcacctcaa catagggaac atcgttgtcg gcaagcaaaa cctgcggaca
      541 aaagaacaag agctgaaaca ccgccaaccc gccaattttg cgataaagtg ccgccacact
      601 aaaaatattt tttttaacca tattcttttt gttttggggt ctatccctcg cgtttgtccc
      661 gaggtaatcc aagggataac gcgagcggtt agacccctcc aacgcattcg aatttaaggg
      721 tctcgtaaat tatagttgac ctcaatcggc gcccaaaatt tgcttgatcg cctcaacgaa
      781 cgccgccgac agcgcgatga tcgccaatcc gaccagagaa aacgtgatga tctgtttgcc
      841 cttggtgatt ttgttttgat cgccgctggc ggtggcaatt tcaaacccgc cccagatgat
      901 caaaatcggg caaatcatca gcgccagccc gaagattacc cccgcaattt taccgacaat
      961 gtcttccacc gtcaaatttc cctgcagtgg gctcaccaag accgcctccc ccgacccgcc
     1021 gccttgatta tccgccgccg gagcgtccgc cgtttcatcg gcccgggcga ttcccgccgg
     1081 caggcacaag agcgaaaaca tcgccgcggc c
//
LOCUS       DAOQWE010000255         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_177000_length_1108_cov_2.542260, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000255 DAOQWE010000000
VERSION     DAOQWE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177000_length_1108_cov_2.542260"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKK37_05170"
     CDS             <1..269
                     /locus_tag="PKK37_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81801.1"
                     /translation="EWQTTGQAIGRYQAYAKVMLDDTVIKEENFQFDILPAGLANSLS
                     ADISNSIGGNYNLILSIAGVGLLLLVGLILGKKKFGKKQTITTT"
     gene            315..>1108
                     /locus_tag="PKK37_05175"
     CDS             315..>1108
                     /locus_tag="PKK37_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNR81802.1"
                     /translation="MLQKTIISLYRSNSFFKKAPALLLAFLLVLPQLFLPALIVAETA
                     PVANPILPQNCGLDLVLVVDQSGSIDADEIQQEKDAFVALIDDLTGTPTEFAVISFNT
                     SAVVRQGFTNDADLAKTAVNSLAYAYNTATNWQDALIKAKSVLPNEDCRHNLIVFASD
                     GNPTVNNENFGTGLVTDNNDLENAIIAANDVKQTYNTRIVTLGIGDDVNEVNLRKISG
                     NVAGDHYKVADFEELKIALRDLAAGMCGGTITVGKFIGKEGNWVLAG"
BASE COUNT          288 a          183 c          310 g          327 t
ORIGIN      
        1 ttgagtggca aactaccgga caggccattg gtagatatca ggcatatgca aaagttatgt
       61 tggatgacac cgttattaaa gaagaaaatt ttcaatttga tattttgccg gccggattgg
      121 ccaattcttt atcggctgac atctctaatt ctatcggcgg aaattataat ttaattttat
      181 ccattgcagg agtgggattg cttttgttgg tggggttgat tttgggtaaa aagaaatttg
      241 ggaaaaagca aaccataacc acaacttagt taaatttaaa ttctaattaa aaattctgcg
      301 attttttaaa tcatatgctt caaaaaacta tcatttcgct ttatcggtct aatagttttt
      361 tcaaaaaggc gccagctttg ttgttggcgt ttttgttagt tttaccgcaa ttgtttttgc
      421 cggcattaat tgtggccgaa accgcgccgg ttgccaatcc gattttgccg cagaattgcg
      481 gtttggactt ggtgctggtg gttgaccagt cggggagcat tgatgccgat gaaatccagc
      541 aggaaaagga tgcgttcgtg gcgttgattg atgatttgac gggaactccc acggagtttg
      601 cggtgataag tttcaatacc tcggcggtgg tgcggcaggg atttaccaac gatgccgatt
      661 tagcaaaaac cgcggtgaat tctttggcct atgcttataa cacggcgaca aattggcaag
      721 atgcgctgat caaagccaag agcgttttac ccaacgagga ctgccggcat aatttgatag
      781 tttttgcctc cgatggcaat cccacggtta ataatgaaaa ttttggcacg ggtttggtga
      841 ctgataacaa tgatttggag aacgcgatca tcgcggccaa tgatgtgaag cagacttata
      901 atacgcggat tgtgactttg gggattggcg atgatgtcaa tgaggtgaat ttgcggaaaa
      961 tttcggggaa tgtggcgggc gaccattata aagtggcgga ttttgaggaa ttgaagattg
     1021 cgttgcggga tttggcggcg gggatgtgtg gggggacgat tacggtgggg aagtttatcg
     1081 gcaaggaggg gaattgggtg ctggcggg
//
LOCUS       DAOQWE010000256         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_178075_length_1104_cov_6.197331, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000256 DAOQWE010000000
VERSION     DAOQWE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178075_length_1104_cov_6.197331"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1104)
                     /locus_tag="PKK37_05180"
     CDS             complement(<1..>1104)
                     /locus_tag="PKK37_05180"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HNR81803.1"
                     /translation="ARIFFEIAAILDAGGESFKPQAYRKAARALLELAEDAALIYARG
                     GLKAVEAIPGVGQSIGQKIEEYVLTGKIDYYRQLKRDFPVDLDEVAGVEGLGPKRAKI
                     LYEKLGVKNLRQLEAAARRGKIAPLFGFGPKSQANILQGIEFKKRSGGRFLLSEVFPA
                     AFDAKEKISALPQIARVEIAGSLRRAKETIGDVDLLAAPKLGADAKDAAAIMDFFSTL
                     AGIEKIWAKGPTKTSVRMAAGFDIDLRLVGEQSFGAAWQYFTGSKEHNIALRRLAIQK
                     GFKLSEYGLFENDKMAAGETEEGIYQKLGLDWIAPEMRENAGEIEAAQNGKLPRLLEC
                     GSLKGDLHCHSNWSDGKNSIEELAAAAMAMGYE"
BASE COUNT          242 a          340 c          244 g          278 t
ORIGIN      
        1 attcataacc catcgccatg gccgcggccg ccaattcctc aattgaattt ttgccatccg
       61 accagtttga atggcagtgc aagtcgcctt tcagactgcc acattccaaa agccgcggca
      121 atttgccgtt ttgcgcggct tcaatttcgc cggcattttc gcgcatttcc ggcgcgatcc
      181 aatccagtcc caatttttga taaattccct cttcggtttc gcccgcggcc attttatcgt
      241 tttcaaacaa gccatattca gaaagcttga atcctttttg gatggccaac cggcgcaagg
      301 cgatattatg ctccttggag ccggtgaaat actgccacgc cgcgccgaaa gattgctcac
      361 ccaccaaccg caaatcaatg tcaaagccgg ccgccatccg caccgaagtt ttggtgggcc
      421 ctttggccca tattttttca atcccggcca gcgttgaaaa aaaatccatt atcgcggcgg
      481 cgtcttttgc gtcggcccca agttttggcg cggccagcag atcaacgtcg ccgatggttt
      541 ctttggcgcg gcgcagggaa ccggcaattt ccacccgcgc gatttgcggc aatgccgaaa
      601 ttttttcttt ggcgtcaaac gccgccggaa aaacttcgct caacaaaaac cggccgccgg
      661 atcgtttttt gaattcaatg ccttgcaaaa tattggcctg cgactttggg ccgaatccga
      721 aaagcggcgc gattttgccg cggcgggccg ccgcttcaag ctgtcttaaa tttttaacgc
      781 ccaatttttc atacaatatc ttggcgcgtt tcggccccaa tccttccact ccggccactt
      841 catccaaatc aaccggaaaa tctcttttca attgccgata atagtcaatc ttgccggtca
      901 aaacatattc ttcaattttt tggccgatgc tttggcccac ccccggaatc gcttccacgg
      961 ccttcagccc gccccgggcg taaatcagcg ccgcgtcttc agccaattcc aaaagcgcgc
     1021 gcgccgcctt gcgataggcc tgcggcttaa atgattcgcc cccggcatcc aaaatggcgg
     1081 cgatctcaaa aaatattctg gcca
//
LOCUS       DAOQWE010000257         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_178080_length_1104_cov_5.051478, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000257 DAOQWE010000000
VERSION     DAOQWE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178080_length_1104_cov_5.051478"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1104)
                     /locus_tag="PKK37_05185"
     CDS             complement(<1..>1104)
                     /locus_tag="PKK37_05185"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HNR81804.1"
                     /translation="ARIFFEIAAILDAGGESFKPQAYRKAARALLELAEDAALIYARG
                     GLKTVEAIPGVGQSIGQKIEEYVLTGKIDYYRQLKRDFPVDLDEVAGVEGLGPKRAKI
                     LYEKLGVKNLRQLEAAARRGKIAPLFGFGPKSQANILQGIEFKKRSGGRFLLSEVFPP
                     AIDAKEKISVLPQITRVEIAGSLRRAKETIGDVDLLAALKIGAKEKDATAIMDFFSTL
                     SGIEKIWAKGSTKTSVRMAAGFDIDLRLVPEGSFGAAWQYFTGSKEHNIALRRLAIQK
                     GFKLSEYGLFENDKMAAGKTEEEIYQKLGLDWIAPEMRENAGEIEAAQNGKLPRLLEY
                     GSLKGDLHCHSNWSDGKNSIEELAAAAMAMGYE"
BASE COUNT          250 a          325 c          237 g          292 t
ORIGIN      
        1 attcataacc catcgccatg gccgcggccg ccaattcctc aattgaattt ttgccgtccg
       61 accagtttga atggcagtgc aaatcgcctt tcagactgcc atattccaaa agccgcggca
      121 atttgccgtt ttgcgcggct tcaatttcgc cggcgttttc gcgcatttcc ggcgcgatcc
      181 aatccagtcc caatttttga taaatttcct cttcggtttt gcccgcggcc attttatcat
      241 tttcaaacaa gccgtattcg gaaagcttga atcctttttg gatggccaac cggcgcaagg
      301 cgatattgtg ttccttggag ccggtgaaat actgccacgc cgcgccaaaa gacccttcgg
      361 gcaccaaccg caaatcaatg tcaaaaccgg ccgccatccg cacggaagtt ttggtggatc
      421 ctttggccca tattttttca attccggaca atgttgaaaa aaaatccatt atcgcggtgg
      481 catctttttc tttggcccca atttttaacg cggccaataa atcaacgtcg ccgatggttt
      541 ctttagcgcg gcgcagggaa ccggcgattt ccacccgcgt aatttgcggc aataccgaaa
      601 ttttttcttt ggcatcaatc gccggcggaa aaacttcact cagcaaaaac cggccgccgg
      661 atcgtttttt gaattcaatg ccttgcaaaa tattggcctg cgactttggg ccgaatccga
      721 aaagcggcgc gattttgccg cggcgggccg ccgcttcaag ctgtcttaaa tttttaacgc
      781 ccaatttttc atacaatatc ttagcgcgtt tcggccccaa tccttccacc ccggccactt
      841 cgtccaaatc aaccggaaaa tctcttttca attgccgata ataatcaatc ttgccggtca
      901 aaacatactc ttcaattttt tggccgatgc tttggcccac gcctggaatc gcctccacgg
      961 ttttcagccc gccccgggca taaatcagcg ccgcgtcttc ggccaattcc aaaagcgcgc
     1021 gcgccgcctt gcgataggcc tgcggcttga atgattcgcc cccggcatcc aaaatggcgg
     1081 cgatctcaaa aaatattctg gcca
//
LOCUS       DAOQWE010000258         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_180917_length_1094_cov_2.960539, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000258 DAOQWE010000000
VERSION     DAOQWE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180917_length_1094_cov_2.960539"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKK37_05190"
     CDS             complement(<1..192)
                     /locus_tag="PKK37_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81805.1"
                     /translation="MATKKITEFDTHSIAAADYLLFSNAAGTATYKTTLLGLLSNLTL
                     SKETVGFTIAGGTTPKTLTL"
     gene            complement(197..343)
                     /locus_tag="PKK37_05195"
     CDS             complement(197..343)
                     /locus_tag="PKK37_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81806.1"
                     /translation="MPTKINSFARDVLLVAQESTKQSPIYILSEQEDYILLGENAISN
                     QIKR"
     gene            complement(347..1048)
                     /locus_tag="PKK37_05200"
     CDS             complement(347..1048)
                     /locus_tag="PKK37_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81807.1"
                     /translation="MTFSEIVAKIDRACGTTATSYTLANKATDVNLAFRDLVAIALKN
                     RKIAYDDNNHTANPFIILDLEDGYRDYYLDKDEQSNIILSINKLMVADQSGVFHEMER
                     VNMETDGNTAGFWDGQDIEGQPSKYSQVGMAIFLDPVPSYDATGGIKAYIDREPDFFT
                     SSDTTKVAGIDPLCQDYLWLKPSYEYCRDHDKKNSERLFRDMNLALQRAEARLLGDKT
                     KDIPRRIIPVFSNNR"
BASE COUNT          273 a          259 c          231 g          331 t
ORIGIN      
        1 caaagtcaaa gtctttggcg ttgtcccgcc ggcaatagta aagccgacag tttccttgga
       61 aagcgtcaaa ttactcaaca agcccaacaa agtggtttta taggttgccg ttcccgcggc
      121 attagaaaac aaaagatagt ccgccgccgc gattgaatga gtatcaaatt cggttatttt
      181 ttttgttgcc atagatttat ctttttattt gattgcttat cgcattttcc cccaacaaaa
      241 tataatcttc ctgttcgctt aaaatatata tcggcgattg tttcgtgctt tcttgcgcca
      301 ctaaaagaac atccctcgca aaactattga ttttggtcgg catagtttat cggttattgg
      361 aaaacacggg aattatccgc cgcgggatgt cttttgtttt gtcccccaaa agcctcgcct
      421 cggcgcgttg caatgccaaa ttcatatcgc ggaaaagcct ctcggagttc tttttgtcgt
      481 ggtcgcggca atactcataa gatggtttaa gccacaaata atcttggcaa agcgggtcaa
      541 ttccggccac ttttgtggta tccgatgaag tgaagaagtc cggctcgcgg tcaatgtatg
      601 ccttaatccc tccggtagcg tcatagctcg gcacagggtc aagaaagatt gccatcccca
      661 cttggctgta tttggacggt tgtccttcaa tatcttgccc gtcccaaaat ccggcggtgt
      721 ttccgtccgt ttccatattc acccgttcca tttcgtggaa tactcccgat tggtcagcca
      781 ccatcaattt gttgattgaa aggataatgt tgctctgttc gtctttatcc aaataataat
      841 cacggtatcc atcctccaaa tccaaaatga tgaaaggatt ggcggtatga ttattgtcgt
      901 cataagcaat ctttcggttc ttcaaagcaa tcgccaccaa atcgcgaaaa gcaaggttaa
      961 catccgttgc cttgttcgcc agcgtgtatg atgtcgcggt agtcccgcaa gcgcggtcta
     1021 tctttgctac gatctcgcta aaagtcatat tgattggaat ttaattagct catctttggg
     1081 cgcagaattc aacg
//
LOCUS       DAOQWE010000259         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_181708_length_1091_cov_6.514479, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000259 DAOQWE010000000
VERSION     DAOQWE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181708_length_1091_cov_6.514479"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..957)
                     /locus_tag="PKK37_05205"
     CDS             complement(82..957)
                     /locus_tag="PKK37_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNR81808.1"
                     /translation="MENQNNNVCGIGGCPSGPCRIVVGALAIALAVYVAALAVNAIKS
                     NKYIGRQVANQATISVSGDGEVYKTPDLAVMSFSVVSESKTVAEAMEDNTKKMNAITD
                     VMKSMGVAENDLQTTNFSINPRYDYVRAAVPAPASADGAETAIDGEYYYPSGKRTLSG
                     YDVNQTLTIKIRQENMGKIGQIIQEATASGANQVGSLQFTLDDPDAAQAEARELAIVE
                     AKEKAEILAKQLNVKLVRIISYNDGGYSPTYRLNYDAKEEAYGMGGAASVPAPDIQAG
                     ESKISMSVNITYEIE"
BASE COUNT          223 a          315 c          253 g          300 t
ORIGIN      
        1 tccatattgc gcccatatta acaaaaaacc gccccccgtg aaagggcggg ggcggttttt
       61 gtcgccggtt aaacgtgaag cctattcaat ttcgtaggta atattcacgc tcattgaaat
      121 cttgctttcg cccgcctgga tgtccggcgc gggcaccgac gcggcaccgc ccatgccata
      181 ggcctcttcc ttggcgtcat aattcaagcg gtaagtcggc gaatacccgc cgtcgttata
      241 gctgatgatg cgcaccagct tcacgttcaa ttgcttggca agaatttcgg ctttttcctt
      301 ggcctcaaca atggcaagtt cccgtgcttc ggcttgcgcg gcatcgggat cgtccagcgt
      361 gaattgcaaa ctgccgacct gattggcgcc cgacgccgtg gcttcctgga ttatctgtcc
      421 gattttcccc atattttcct gacggatttt gatcgtcaaa gtttgattaa cgtcataacc
      481 ggaaagcgtc cttttccccg acggataata atattctccg tcaatcgcgg tctcggcgcc
      541 atcggccgac gcgggcgccg gcaccgcggc tctcacgtaa tcatagcgcg gattgatgga
      601 aaaattcgtg gtctgcagat cgttttcggc cacccccatg ctcttcatca catcagtaat
      661 cgcgttcatt tttttggtgt tgtcctccat ggcttccgcc acggttttgg attcgctcac
      721 caccgaaaag ctcatcaccg ccagatccgg cgttttgtag acttcaccat cgccggaaac
      781 cgaaatcgtc gcctggttgg caacctgccg gccgatatat ttgttggact taatggcatt
      841 gaccgccaat gccgccacat aaaccgccag cgcgattgcc agcgccccga caacaatccg
      901 gcacggcccg ctcggacaac cgccgatacc gcaaacatta ttattttgat tctccataat
      961 tttaattttt aattttggtt tgatttttat tgattgattc gcgacctttg ttttgattat
     1021 aacagattcc cgcgcgcaaa caaaaacccg ccgggttttg ccggcagagt tttcgctctt
     1081 tgttttgcgc t
//
LOCUS       DAOQWE010000260         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_182565_length_1088_cov_6.173282, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000260 DAOQWE010000000
VERSION     DAOQWE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182565_length_1088_cov_6.173282"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PKK37_05210"
     CDS             complement(<1..959)
                     /locus_tag="PKK37_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81809.1"
                     /translation="MELFIWLLIFAVSLAALLIGAVWFSAAASRFLNKAAAAGFAAIA
                     VGAALPELALALAAVLSGRPELVLPIVLGSSVANIFLVVGASAAAAKNLPIVRECAEA
                     EAPLLAGSAAILWFSAADGIVFFEEGLLMVVAFLVCVVFLAARGRINRFTPRDIVAPG
                     FLAAGRGLVEIVGARFGRGFDGHAKNTARALLRGMAGAVLLALAAIFAVEALAGAATA
                     MAISPVLLAFSVLAATAALPEIWGSLKIIAKKRYELVMGNVFSGTAVNVLLVTGVAAM
                     FSPLPVGASAAAVGLPFFAAAVALLTVSGFSRKISSGQGWLYL"
BASE COUNT          302 a          356 c          263 g          167 t
ORIGIN      
        1 aacagataca gccagccctg tccggagctt attttgcgcg aaaaccccga gactgtcaac
       61 agcgcgaccg cggcggcgaa gaacggcaaa ccgaccgcgg cggcggaagc gcccaccggc
      121 agaggcgaaa acattgccgc aacaccggtg accagcaaaa cattcaccgc cgtgccggaa
      181 aaaacgtttc ccatcaccaa ttcgtagcgt tttttggcga taattttcaa gcttccccaa
      241 atttccggca atgcggcggt ggcggctaaa accgaaaacg ccaacagcac gggacttatc
      301 gccatcgctg tcgcggcgcc ggccagcgcc tcaacggcga aaatcgccgc cagcgccagc
      361 agaaccgcgc cggccatccc ccgcaaaagc gcccgggcag tgtttttggc gtggccgtca
      421 aaaccccggc caaaccgggc gcccacgatt tcaaccagtc cgcggccggc cgccaaaaat
      481 cccggcgcga cgatatcacg gggagtgaac cggttaatcc gcccgcgcgc cgccaaaaat
      541 accacgcata ccaaaaacgc aaccaccatc aacagcccct cttcaaaaaa aacaatcccg
      601 tcggcggcgg aaaaccataa tatcgcggcg cttcccgcca gcaacggcgc ctcggcctcc
      661 gcgcactcgc ggacaatcgg taaatttttt gccgccgccg cggacgcgcc aaccaccaaa
      721 aaaatattgg cgaccgacga ccccaaaacg attggcagaa ccaattccgg gcggccggac
      781 aaaaccgccg ccagcgccag cgccagttcg ggcaatgccg cacccacggc aatcgcggca
      841 aatcccgcgg cggcggcctt gttcaaaaac cgggaggcgg cggcggaaaa ccacaccgcg
      901 ccgattaaca gcgccgccag cgacacggca aatatcaata gccaaatgaa aagttccacg
      961 gacaaaattg aaaatgtaaa tatcaaaatg aaaaatgaca atgtaaaact gcaaaatgtc
     1021 cggcttccga cattttaaca ttttaatctg tcattttgat ctttgatttt tcaattttac
     1081 atttatta
//
LOCUS       DAOQWE010000261         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_183453_length_1085_cov_8.540777, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000261 DAOQWE010000000
VERSION     DAOQWE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183453_length_1085_cov_8.540777"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1085
                     /gene="metG"
                     /locus_tag="PKK37_05215"
     CDS             <1..>1085
                     /gene="metG"
                     /locus_tag="PKK37_05215"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNR81810.1"
                     /translation="VNAKPHLGFALEIIQADVLARWQRSLGKEVFFNTGTDEHGLKIY
                     QKAAELGKTPQEYCDESAQHFKALKEKLDLSFDNFIRTTDAHHIAAAQEFWKRCDANG
                     DIYKKVYKAKYCVGCELEVTDSQLVNGRCPVHPNKDVETIEEENYFFRFSKYQQPLLD
                     FYNANPDFVIPEGRFNEIKSFVAGGLQDFSISRVKEKMPWGIAVPGDEGQVMYVWFDA
                     LVNYISCLNWPEDEKKFEEFWGTKENRNAIQVAGKDNLRQQTAMWQAMLMSAGLPNSK
                     QIFIHGFITAGGQKMSKSLGNVIDPVRLVDKYGTDAARYFLLREISADEDGDFSYDRF
                     EERYNADLAKGIGNLAARVTTVAKKIGA"
BASE COUNT          289 a          224 c          300 g          272 t
ORIGIN      
        1 gtgaatgcca agccgcatct ggggtttgcg ctggagatta tccaggcgga tgttttggcg
       61 cgctggcaga gatcgcttgg gaaagaagtg ttttttaata cgggcacgga tgaacacggg
      121 ctaaagattt atcaaaaggc ggcggaactg ggtaagacgc cccaagagta ttgtgatgag
      181 tccgcccaac atttcaaagc gttaaaagaa aaattggatt tgtcgtttga taattttatc
      241 cgcacgaccg atgcgcatca tatcgcggcg gcgcaggaat tttggaaacg gtgcgatgcc
      301 aacggcgata tttacaaaaa ggtttacaaa gcgaagtatt gcgttggctg tgaactggaa
      361 gttaccgatt cccagctggt gaatggccgg tgcccggtcc atcccaacaa ggatgttgaa
      421 accattgaag aagaaaatta ttttttccgg ttttccaaat accaacagcc gttgctggat
      481 ttttataacg ccaatcctga ttttgtgatt cccgaggggc ggttcaacga aataaaaagt
      541 tttgttgccg gtggattgca ggatttttcc atttcccgcg tcaaagaaaa aatgccctgg
      601 ggaattgcgg tgcccggcga tgaggggcaa gtgatgtatg tttggtttga tgccttggta
      661 aattatattt cctgtttaaa ctggcctgaa gacgaaaaga aatttgaaga attttgggga
      721 accaaagaaa atcgcaatgc catccaggtc gcgggcaagg ataatttgcg ccagcaaacc
      781 gcgatgtggc aggcgatgtt aatgtcagcc gggttgccca attccaaaca gattttcatt
      841 cacggattta tcaccgccgg cgggcagaaa atgtccaaat ctttgggcaa cgtgattgat
      901 ccggtccggc tggttgataa atacggcacc gatgcggcgc gttattttct gttgcgcgaa
      961 atttccgccg atgaagacgg cgacttttca tatgatagat ttgaagaacg ctacaacgcc
     1021 gatttggcca agggcatcgg caatctggcg gcgcgggtga ccaccgtggc aaaaaagatc
     1081 ggcgc
//
LOCUS       DAOQWE010000262         1069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_188213_length_1069_cov_6.067061, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000262 DAOQWE010000000
VERSION     DAOQWE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188213_length_1069_cov_6.067061"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..518)
                     /locus_tag="PKK37_05220"
     CDS             complement(3..518)
                     /locus_tag="PKK37_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81811.1"
                     /translation="MNRKNKIIVVIVSILLAVVSLWYFFADDLFLFILSRTVPPAEPM
                     PAEQRMPCGGNPASFVELGSPYLTKSTAEFTTVGGEIYVIARRYEHGILLPERGGSGI
                     YVGHLNQLPVWDKQTSKIANVYKEITIKEKDYGALTLPAGRYWLWSSSGGDVVIYSCE
                     EGGVSDPKPVR"
     gene            complement(525..>1069)
                     /locus_tag="PKK37_05225"
     CDS             complement(525..>1069)
                     /locus_tag="PKK37_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81812.1"
                     /translation="QSGRTYANNQILPIDFRVVDNQPANDIVQTEIYLNGAVVSGNSV
                     DLSLQKTGNHRLEINAQDDAGNIARAESDFAVSADIGSIIANISHYYDLKMIKLLSTR
                     NALLVAAKSLKLQLEALDRLKQGALPITTKNSLIKLSQKSINRQIDALVVLVNNLPAK
                     SIDARAKELLADSLQYIKIK"
BASE COUNT          301 a          251 c          223 g          294 t
ORIGIN      
        1 aactaccgca ccggcttggg atcgctcacc ccgccctctt cgcaactgta aatgacaacg
       61 tccccgccgg aacttgacca aagccaatac ctccccgccg gcaaagttag tgcgccgtaa
      121 tctttttctt tgattgtaat ttctttataa acattagcta ttttgcttgt ttgcttatcc
      181 cagaccggta attggtttaa gtgaccaaca taaataccag aaccgcctcg ttctggcaat
      241 aagataccgt gttcatatct tcgtgcaata acatatatct cgccaccaac ggtggtaaac
      301 tctgcggtag atttggttaa atacggcgac cccagttcca caaagctggc gggattgccg
      361 ccgcaaggca tccgttgttc cgccggcatc ggttccgccg gcggaacagt acgcgagaga
      421 ataaaaagaa acaaatcatc agcgaaaaaa taccataaag aaacaaccgc cagcaagatt
      481 gaaacgataa cgacaataat tttatttttt ctgttcattt gaatttattt gattttaatg
      541 tattgcaagc tgtcagccaa aagctctttg gcgcgcgcgt caattgattt cgccggcaga
      601 ttattaacca aaacaaccag cgcgtcaatc tggcggttaa tggatttttg cgataatttg
      661 atcaaagaat tttttgttgt tatcggtaag gcgccctgtt ttaacctgtc cagcgcctcc
      721 aattgaagct ttaacgattt ggccgccacc aacaatgcat tgcgcgttga taaaagttta
      781 atcattttga gatcataata atggctgata ttggcgataa ttgaaccaat atcggccgaa
      841 acggcaaaat ccgattcggc acgggcaata ttgccggcat cgtcttgggc attaatttca
      901 agccgatgat tgccggtttt ttgcaatgac aaatcaacgg aattccccga aacaacagct
      961 ccgttcaaat aaatttcggt ttggacaata tcgtttgcgg gttgattgtc aacaacccga
     1021 aaatcaatcg gcagaatttg attgttggca taagtccggc cggattgcg
//
LOCUS       DAOQWE010000263         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_189840_length_1064_cov_2.549058, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000263 DAOQWE010000000
VERSION     DAOQWE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189840_length_1064_cov_2.549058"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PKK37_05230"
     CDS             <1..618
                     /locus_tag="PKK37_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81813.1"
                     /translation="RFGVTYAIIGLIVMNLLLFSFSTESGLRFLEEMTTPGGIFDVWQ
                     NLVALLVVASLSGAAIVYGWIGKALIKWEAFFATVLFFIFQLLTALPNQSLLIDNHYT
                     SQLTAAGLFWAAIFNFVLLSEILGAIFAGYLRREIWMINFGTVLLIIFTAVKYFDWFF
                     KFMDKSVFFIVAGIMLFAIGWAMERGRRYLAQTIQPPITDNQENQ"
     gene            615..>1064
                     /locus_tag="PKK37_05235"
     CDS             615..>1064
                     /locus_tag="PKK37_05235"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNR81814.1"
                     /translation="MKTNLSPSIKFLIAIFVQLAIVFGLVVFNAAMLARGTEILLPIE
                     PFDPRDPLRGDYAILRYEISNIKEDFFVNKGNELKGNDLIYVSLEKRDNYWIAKGASV
                     QKPKEGLFIRGNAGYHYSWIIGIPVEYGIEEYFIPEGTGAALEKKMRA"
BASE COUNT          264 a          195 c          262 g          343 t
ORIGIN      
        1 cgctttggcg ttacttacgc gattattggt ttaatcgtga tgaatctttt gctcttcagc
       61 ttttccactg aatcgggatt gagatttttg gaagaaatga caacgcccgg cggaattttt
      121 gatgtttggc aaaatctagt tgccctgctg gttgttgcga gcttgtcggg agcggcgatt
      181 gtttatggct ggataggtaa agctctcata aaatgggaag cgttttttgc cactgtctta
      241 ttttttattt ttcagttatt aaccgctttg cccaaccaat cgttattaat cgataaccat
      301 tacaccagcc agcttaccgc cgcgggatta ttttgggcgg cgatatttaa ttttgtgttg
      361 ttgtctgaaa ttttgggggc aatttttgcc ggttatctgc gccgcgaaat ttggatgatt
      421 aatttcggca cggtgttatt gataattttt accgcggtga aatattttga ctggttcttc
      481 aagtttatgg ataagagcgt tttctttatt gtcgccggaa ttatgctgtt tgccatcggt
      541 tgggcgatgg agcggggacg ccgctatctg gcgcaaacca tccagccgcc tataacggat
      601 aatcaagaaa accaatgaaa accaatctgt cgccgtcaat caagttttta atcgcaattt
      661 ttgtccagct ggcaatcgtt ttcggcttgg tggtttttaa tgccgccatg ctggcgcgcg
      721 gaaccgaaat tttgttgccg attgaaccgt ttgatccgcg ggaccccttg cgtggcgatt
      781 acgcgatttt gagatatgag atatcaaaca tcaaagaaga tttttttgtt aacaaaggaa
      841 acgaattaaa aggaaatgat ttgatttatg tttctctgga aaaaagggat aattattgga
      901 tcgctaaagg cgcgagtgtc caaaaaccga aagaagggct gtttatcagg gggaatgccg
      961 gttatcatta ttcttggatt attggaattc ccgttgaata tggtattgaa gaatacttta
     1021 ttcccgaagg aaccggcgcc gcattggaga aaaaaatgcg cgcg
//
LOCUS       DAOQWE010000264         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_190512_length_1062_cov_1.919563, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000264 DAOQWE010000000
VERSION     DAOQWE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_190512_length_1062_cov_1.919563"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKK37_05240"
     CDS             <1..642
                     /locus_tag="PKK37_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81815.1"
                     /translation="SFNTITPEITTVRVVEVKPSLQNATFDVLALSVGGGVNAAVLVQ
                     NATFAVSEVSVGMGADTVAETQNAAFAVQEVEITANWLVTVEPNVLNAEFGVISPTIV
                     GGIEITAQLQNATFDLPAASVLAIQNITIAPEVLAGVLAMPAHSVSAAIGAYVSAGLQ
                     NAAFAILGHTVGLGATLCPNTINTVFYLLRVPTVYTDKYSAKGTIYTDKYSKI"
     gene            639..>1062
                     /locus_tag="PKK37_05245"
     CDS             639..>1062
                     /locus_tag="PKK37_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81816.1"
                     /translation="MSNTLLSALVTKLDNKIAASKTNGFFSTAQKEASLNTAGERVCN
                     YRAWKALEDSKKTTTVADQEYYDEPDEFQADSIYAMKIDGEKYDKKDWMDFQDYVNNE
                     SSDKVFATHDGLYFINPIPDDSNLDLCIYGIKKWVALAS"
BASE COUNT          301 a          214 c          282 g          265 t
ORIGIN      
        1 tcattcaata cgattacccc ggagattacg acggtcagag tggttgaggt aaaacccagt
       61 ttgcaaaatg ccacttttga tgttctggcg ctatcggttg gcgggggagt gaatgcggcg
      121 gttttggtcc aaaacgcgac ttttgcggtc tcggaagttt cggtcggaat gggcgcggat
      181 acggttgcgg aaacacagaa cgcggcgttt gcggtgcagg aggtagaaat taccgccaac
      241 tggttggtaa ctgttgagcc gaatgttttg aatgctgaat ttggcgttat cagtccaact
      301 attgttgggg gaatagaaat taccgcacag ttgcagaatg caacatttga tttaccagcc
      361 gcttcggtat tggcaattca aaatatcacg attgcgccgg aggtattggc cggagttttg
      421 gcgatgccgg cacattcagt ttccgcggca atcggcgcgt atgtttccgc gggtttgcag
      481 aatgcggcgt ttgcgatatt ggggcatacc gttggattgg gagcgacatt gtgcccaaac
      541 acgataaaca ccgtttttta tcttttgcga gtgccgacag tctacaccga taaatattct
      601 gcaaaaggga caatctacac cgataaatat tcaaaaatat gagcaacaca cttttaagcg
      661 cattggttac caaattggac aataagatcg cggcttcaaa aaccaacggg ttttttagca
      721 ccgcgcaaaa agaggcatcg ttgaataccg ccggcgagcg ggtttgcaat tatcgggcat
      781 ggaaggcact ggaggactca aaaaaaacca caacagtcgc agaccaggaa tattatgacg
      841 aaccggacga atttcaggcc gacagcatct atgcgatgaa aattgacggg gaaaaatacg
      901 acaaaaagga ttggatggat tttcaggatt atgtcaataa cgaaagtagc gacaaagtat
      961 tcgcaactca tgacgggctt tattttatta atccgatacc cgacgatagc aatttggacc
     1021 tttgcattta tggaatcaaa aaatgggtag ctttggcaag cg
//
LOCUS       DAOQWE010000265         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_191255_length_1059_cov_8.585657, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000265 DAOQWE010000000
VERSION     DAOQWE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191255_length_1059_cov_8.585657"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1059)
                     /locus_tag="PKK37_05250"
     CDS             complement(<1..>1059)
                     /locus_tag="PKK37_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR81817.1"
                     /translation="VREEAKEAIQTCYRAGMRPIIITGDHGLTAKAIAKKLGIDVGNE
                     NIMEGKDLEKMSEAQFAKKIGDIQIYARVEPKHKLRIIEAWQKRGEVVAMTGDGINDA
                     PALKQANIGVALGSGTEVAKEVADIVLLTDDFSVIVAAVEEGRAILDNIRKIVTYLLS
                     DSFTEIVLIGSSVLLGLPLPVTAVQILWINLIEDGLPNVALAFEPKEKDLMSQKPQGL
                     DIPLLTREMKVIIFIVGLLTDLLLLGLFLWLVGKNYDIKYIQTMIFAGLAIDSIFYVF
                     CCKSLRKNLWQIDIFNNKLLIVAWFVGLAGLLSAIYIPALNQLLGTVPLPASSWLVIV
                     GLAIANVGLIEAAKYYFI"
BASE COUNT          273 a          309 c          217 g          260 t
ORIGIN      
        1 cgatgaaata gtatttggcg gcttcaatca accccacgtt ggcaatggcc agtcccacaa
       61 tcaccaacca ggaagatgcc ggtaaaggca cggtgccgag caattgattc aacgcgggaa
      121 tataaatcgc agaaagcaat cccgctaacc cgacaaacca cgcaacaatc aaaagcttgt
      181 tgttgaagat atcaatctgc cacaaatttt tcctcaaact cttacagcaa aacacataga
      241 aaatggaatc aatggccagt ccggcgaaaa tcatcgtttg aatgtatttt atatcataat
      301 tcttgccgac gagccaaagg aacaatccca aaagcaacag atccgtgagc agtccgacaa
      361 tgaagataat caccttcatt tcccgcgtga gcaagggaat gtccagcccc tgcggtttct
      421 ggctcatcaa atctttttct ttgggctcaa aggccagcgc gacattgggc agtccgtcct
      481 ctatcagatt tatccacaga atttggaccg cggtcaccgg caaaggaagc cccaacaaca
      541 cgcttgatcc gatcaaaact atttcggtga agctgtccga caacagatag gtgacaatct
      601 tgcgaatatt gtccaaaatg gcgcgccctt cttccaccgc cgccacgata acggaaaaat
      661 cgtcggtgag caggacgata tcggccacct ccttggccac ttcggttccc gatcccagcg
      721 ccactccgat gttggcctgt tttaacgcgg gcgcgtcgtt gatcccgtcg ccggtcatgg
      781 ccaccacctc cccccttttt tgccaagctt caataatcct taacttatgc ttcggttcca
      841 cccgggcata gatttgaata tcgccgattt tcttggcaaa ttgcgcctcc gacatttttt
      901 ccaaatcttt gccctccatt atgttttcgt ttccgacatc aatgcccaat ttcttggcga
      961 tcgccttcgc cgtcaagccg tgatcacccg tgataataat cggcctcatt cccgcccgat
     1021 aacaggtttg tatcgcttcc ttggcttcct cgcgcaccg
//
LOCUS       DAOQWE010000266         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_191329_length_1059_cov_2.736056, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000266 DAOQWE010000000
VERSION     DAOQWE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191329_length_1059_cov_2.736056"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKK37_05255"
     CDS             complement(<1..457)
                     /locus_tag="PKK37_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNR81818.1"
                     /translation="MAIGPGFKRAGMPHEKEMEIAALGCMLTGYPEAVSCVCEMLDLS
                     CFYDPQHQAIFKAGASLYQRGAAVDILTVRDELEQIGQLEKLEDPDYLSYLIERTIST
                     ANIEQYCKILRDSAGSRKMLTLFQDAINSILENPQRPDVDEIRQKFADIA"
     gene            complement(458..946)
                     /locus_tag="PKK37_05260"
     CDS             complement(458..946)
                     /locus_tag="PKK37_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81819.1"
                     /translation="MENISKNKRGRPQILRRDTLRASYYDKGRRQQDNMAYTQLFGML
                     VYPDGSAGEPLDPIYLFLWDNKKVKIKATVSAELGRLAVRSGDDVALQAARVICEKKM
                     NTARALAFLRRFRKTKTGAAWSVLGKILRLIDDSLLSADELETLRQNLNDCLSPQNQE
                     GE"
BASE COUNT          234 a          298 c          252 g          275 t
ORIGIN      
        1 cggcgatatc tgcgaatttt tgccggattt cgtccacgtc cggccgctgc ggattctcca
       61 ggattgaatt gatagcgtcc tggaataaag tcagcatttt cctcgatccg gcgctatccc
      121 ggaggatctt gcaatattgt tcgatattcg cggttgaaat ggtccgctca atcaaataac
      181 ttagatagtc cggatcctcc agcttttcaa gctggccgat ctgttccagt tcgtcccgaa
      241 cggtcaagat atcgaccgcc gcgccgcgct gatataggga ggcgccggct ttgaaaatcg
      301 cctggtgttg cggatcatag aaacaagaca gatccaacat ttcacaaaca caagaaacgg
      361 cttcggggta tccggtcaac atacaaccca gcgccgcaat ttccatttct ttttcgtgcg
      421 gcattcccgc ccgcttgaat ccgggtccaa tcgccattta ttcgccctct tgattttggg
      481 gtgataaaca atcattcaag ttttggcgga gggtttccag ttcgtccgcc gataacaagc
      541 tatcatcaat cagccgcaat atttttccca gaacggacca agcggcgccg gttttcgttt
      601 ttctgaatcg ccgcaaaaac gccaaagccc gcgccgtgtt catctttttt tcacagataa
      661 cccgcgccgc ctgtaatgcg acatcatccc cggaccggac cgccagccgt ccaagttcgg
      721 ccgaaacggt tgccttgatc tttacttttt tgttatccca caaaaaaaga taaatcgggt
      781 ccagcggttc gccggcggat ccgtccggat agaccaacat tccgaaaagt tgcgtatatg
      841 ccatattatc ttgctggcgc cgccccttgt cataatatga cgcccggagg gtgtcgcgcc
      901 gcaatatttg cggccggccg cgtttatttt tggaaatgtt ttccattaat aggcgccgcg
      961 gctaaaaatc aagatcatcc tggaccgccg gcgccggttt ggccgcggcc gcgtctttgt
     1021 cgcgcgcttt cagatagacg taaaaatccg gatctttct
//
LOCUS       DAOQWE010000267         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_191347_length_1059_cov_2.581673, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000267 DAOQWE010000000
VERSION     DAOQWE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191347_length_1059_cov_2.581673"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1059
                     /locus_tag="PKK37_05265"
     CDS             60..>1059
                     /locus_tag="PKK37_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR81820.1"
                     /translation="MLANPPFMSPKGGIRPHKQFSVQANRSEVLFVDYILEHLRPNGR
                     AGIIVPEGIIFQSATAYKALRKLLIEDGLLAVVSLPPGVFNPYAGVKTSILLFDNGLA
                     KKTKDILFLKIQNDGFDLGAQRRKIDKNDLPQALEIIKEYKTALKENKKFKLNETQNK
                     IAHLVAKDKIAESDDYNLSGDRYIETINFANQKWPLVELKDVCVVDWGNTELTKKSYI
                     ENGEYLGVSAAGCDGRMKHFEHEVGTVVLSAIGANCGRVFYPNENFTAIKNTITFTPE
                     KKKLYPKFLFYILRDNTFPRRGGGQPFMTKGDVKKYKIPLPPLEVQKEIVEQIEVKQ"
BASE COUNT          356 a          189 c          227 g          287 t
ORIGIN      
        1 ctatttttga atacgacacg ctttccagcg aggaaaaatg ggacgaaaat tttgatgtta
       61 tgcttgccaa tccgccgttt atgtcgccaa agggtggcat tcgcccgcat aaacaatttt
      121 ccgtgcaagc caaccgttcc gaagttttgt ttgttgatta tattttggag catttgcggc
      181 caaacggacg agccggcatt atcgtaccgg aaggcataat ttttcaatct gccacagcgt
      241 ataaagcgct cagaaaatta ttgattgaag acggactttt ggcggttgta tcacttcccc
      301 ccggggtttt caatccttat gccggagtaa aaaccagcat tttgcttttt gacaatggtc
      361 ttgccaagaa aaccaaagat attctgtttt tgaaaattca aaacgacggt tttgatcttg
      421 gcgcacaaag gcggaaaata gataaaaatg atttgccgca ggcgttggaa ataatcaaag
      481 aatataaaac cgccttgaaa gaaaataaaa aattcaaact gaacgagaca caaaacaaaa
      541 tcgcccatct tgtcgccaaa gacaaaatcg ccgaaagcga tgactataat ctttcaggcg
      601 atagatacat agaaacgatt aatttcgcca atcaaaaatg gccattagtt gagctgaaag
      661 atgtttgtgt ggttgattgg ggaaacactg aacttactaa aaaatcttat attgaaaatg
      721 gagaatatct tggggttagt gcggcagggt gcgacggcag aatgaaacac tttgagcatg
      781 aggtaggaac agttgtattg tcagcaatag gcgcaaattg tggacgagtt ttttatccaa
      841 atgaaaattt taccgcgata aaaaatacaa ttacatttac gcccgaaaag aaaaaacttt
      901 atccgaaatt tttgttttat attttgagag ataatacatt cccaaggcgg ggaggtggtc
      961 agccctttat gacgaaaggt gatgttaaaa aatataaaat cccacttccg ccgcttgagg
     1021 ttcaaaaaga aattgttgag cagattgaag ttaaacaaa
//
LOCUS       DAOQWE010000268         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_192640_length_1055_cov_2.778000, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000268 DAOQWE010000000
VERSION     DAOQWE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192640_length_1055_cov_2.778000"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1000
                     /locus_tag="PKK37_05270"
     CDS             182..1000
                     /locus_tag="PKK37_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR81821.1"
                     /translation="MIRAVHRESRRREDAETIASRERILRREAEILAADLRRLDRAKT
                     QFLLSTQHHLRSPLSVINGYLSMIDEGSYGKITKKSKEKIGACLEANKKLIRLVDELL
                     DVAHFQMNKSAAEKEPVDAVALAAQVVADLKPAAEVKNLYLKFKKPEAPIPEIMLNPR
                     GIKEALYNIVDNAIKYTQTGGVVVLISVFGEKLRIEIKDTGIGMNEKDREGLFGRVFE
                     RCERAKEANVEGKGIGLYLAAQIIANNGGEISVESEGWGKGSKFIIDFPMEAQR"
BASE COUNT          277 a          178 c          309 g          291 t
ORIGIN      
        1 aatttttgtt gctattgccc tttttaaata tcggctgttt gaaaccaggg ttatcttaac
       61 ggagtttttg gtcgggatga tgggtttagt tcttattttt ttgccgtttt tgatgccaac
      121 agccggattg aagattttaa cgtctttgat ttttatcctt ttttgttttt ttggatatta
      181 cttgatcagg gcggtgcatc gggagagtcg gcggcgggag gatgccgaaa cgattgcgtc
      241 acgggaaagg attttgcgcc gcgaggcgga gattttggcg gcggatttgc ggcggttgga
      301 tcgggcgaaa acccaatttt tgctttccac ccagcatcac ttgcgcagtc cgctgtcggt
      361 gattaacgga tatttgagca tgattgacga gggaagttat ggcaaaatca ccaaaaaatc
      421 aaaagaaaaa atcggtgcgt gtcttgaggc caacaaaaaa ttaatccgtt tggtggacga
      481 gcttctggat gtggcgcatt tccaaatgaa caaaagcgcg gcagaaaaag agccggtgga
      541 cgcagtggcg ctggcggcgc aggttgtcgc ggatttgaaa ccggcggcgg aagtaaaaaa
      601 tttatatctt aaatttaaaa aaccggaggc accgattccc gaaattatgt tgaatccgcg
      661 agggatcaaa gaggcgcttt acaatattgt ggataatgca attaaatata cgcaaaccgg
      721 cggggtggtt gttttgattt cggtttttgg ggaaaaattg cggatagaaa tcaaggatac
      781 ggggattggt atgaatgaaa aagatcggga aggtcttttc ggccgggtct ttgaacgttg
      841 cgagagggct aaagaggcaa atgttgaggg caagggaatc ggcctttatt tggcggcgca
      901 aatcatcgcc aacaacggcg gcgaaatcag cgtggaatcg gagggatggg gaaagggaag
      961 caaattcatt attgatttcc cgatggaagc ccaaaggtag gagggcttgt tttttgccgg
     1021 ttgcgtgcta gtttatcatt atggaaaatt catct
//
LOCUS       DAOQWE010000269         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_192886_length_1054_cov_14.170170, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000269 DAOQWE010000000
VERSION     DAOQWE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192886_length_1054_cov_14.170170"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>1054)
                     /locus_tag="PKK37_05275"
     CDS             complement(102..>1054)
                     /locus_tag="PKK37_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HNR81822.1"
                     /translation="LGDKVAVAKYDCEVKVLKKAKESFAEENKNRYYTLGKDEASLFE
                     PELILDRIFDYLGVKSREFEKFKELENEIVHFKKVRFSDDEKYEEIKRKIEEVRHYPE
                     RQKELDKQYGKIPRKEFERRMTLFEQAGNFEMKNQKIAIKYLANHYYLPVIVSETEKI
                     DYLNHIINVDSEVKFVEQLEEYLAKPDNVFTQFDWWMFSKLDQTLDEVLIPYYNPKEN
                     NIANFRPDFIFWAQKGKRYLILFVDPKGTEHADGYRKIDGYSRIFEISKKKQSKDFSY
                     NGFTINTKLLLKSRRGIAEVLENYKRYWFDNFVDFADKIS"
BASE COUNT          275 a          231 c          151 g          397 t
ORIGIN      
        1 cccccagacc cccttttctt tttgcccgcc cgcattgccc gcttcgcggg cgagccgccg
       61 atttttatac cagcgaaact ggcacactat caatttcaaa attaacttat tttgtctgca
      121 aaatcaacaa aattatcaaa ccaatatctt ttgtaatttt ccaaaacctc tgctattcct
      181 cgtcttgatt tgagcaatag ctttgtattt attgtaaaac cgttgtatga aaaatctttg
      241 ctttgctttt ttttgctgat ttcaaatatc cttgaatagc cgtcaatttt tctgtatccg
      301 tccgcgtgtt cggtcccttt cggatcaaca aacagaatta aataacgctt gcctttttgc
      361 gcccaaaaaa taaagtctgg tctgaaattg gcgatgttat tttctttagg attgtaataa
      421 ggtatcaaaa cctcatccag cgtttggtca agttttgaaa acatccacca atcaaattgc
      481 gtaaatacat tgtcgggttt ggcaagatat tcttccagtt gctcaacaaa ttttacttcg
      541 ctatcaacat tgataatatg attaaggtag tcaatttttt ctgtctccga aactatcacc
      601 ggaagatagt aatggtttgc taaatacttg atagcgatct tttgattttt catttcaaaa
      661 ttccccgcct gttcaaacaa ggtcattcgt ctttcaaatt cttttctggg aattttgcca
      721 tactgcttat ccagttcttt ttgtctttcg ggatagtgtc tgacttcttc tatctttcgt
      781 ttgatttctt catatttttc gtcatcgcta aatctaacct ttttaaaatg cacaatttcg
      841 ttttccaact ccttaaactt ttcaaattct ctgcttttaa ctccgagata atcaaaaatt
      901 ctgtcaagaa ttaattctgg ctcaaagagg gatgcctcat ctttgccaag tgtatagtat
      961 ctatttttgt tttcttccgc aaaactttct tttgcttttt tcaaaacttt cacttcgcaa
     1021 tcatatttcg ccacagcgac tttgtcgcct aaaa
//
LOCUS       DAOQWE010000270         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_193480_length_1053_cov_1.491984, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000270 DAOQWE010000000
VERSION     DAOQWE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193480_length_1053_cov_1.491984"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PKK37_05280"
     CDS             <1..992
                     /locus_tag="PKK37_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81823.1"
                     /translation="NICSGQWSDYWSFRTTGRKPTFGSDAVKMAAPTATLTWEAVSAA
                     KSYRVKVTGDGAPAEEVLAATNQFDFDYPGAKKNYSWKIKTCADSAGTVCGEWQAGTS
                     FNSEDFPAPANLSPAGNITQLPSALSWNGTGSYFWVTAQVTNGEKSTSCDPEWFNKEY
                     AGKTVSGTDLALKAAAKNPSSYCSGDYLFTVRPCFDEKCAARSFNEARQTFSVAGKNE
                     NSGLMVCGQKSDDPGTPYNEKEPCEIKHIFLTLKVIIDFVTFKLALLLLPVMALVTGG
                     LFYLAPDKANLTARAKDIWKKIGIGYAILFFSWIIVSIIMALAGYGALWNQIL"
BASE COUNT          274 a          260 c          267 g          252 t
ORIGIN      
        1 aaaacatttg cagcggccaa tggtccgatt attggtcttt tcgcaccacc ggaaggaaac
       61 cgaccttcgg aagcgacgcg gtaaaaatgg ccgccccgac cgccactttg acttgggagg
      121 cggtttcggc cgcaaaatcg tatcgcgtta aagttaccgg agacggcgcg ccggcggaag
      181 aagtccttgc cgccacaaat caatttgact ttgactatcc cggcgccaaa aaaaactact
      241 cgtggaaaat taaaacttgc gccgacagcg ccggcacggt ttgcggcgaa tggcaagcgg
      301 ggacatcatt caattctgaa gatttccccg cgccggccaa tttatcgccg gccggcaata
      361 ttacacaact gccatccgct ttgtcatgga acggcaccgg cagctatttc tgggtcacgg
      421 cgcaagtaac caacggcgaa aaaagcacca gttgcgatcc ggaatggttc aataaagaat
      481 atgcgggaaa gacagtctcc ggcaccgatt tggcgctcaa ggccgcggca aaaaatccct
      541 cttcctattg ttccggagat tatttattca ccgtgaggcc ttgttttgac gagaaatgcg
      601 cggcgcgcag ttttaacgaa gcgcggcaaa cattttccgt cgctggcaaa aatgaaaatt
      661 ccggactgat ggtttgtggc caaaaaagcg acgaccccgg caccccgtat aacgaaaagg
      721 agccttgcga gattaaacac atatttttaa ctttaaaagt catcattgat tttgtgacgt
      781 tcaaactggc gctcctgctc ttgccggtga tggcgctggt caccggcggc ctgttctatc
      841 tggcgccgga caaagcgaat ttaaccgcgc gcgccaaaga catctggaaa aaaatcggca
      901 tcggttacgc cattttgttt ttttcgtgga ttattgtttc cataataatg gcattggcgg
      961 gctacggggc gttgtggaac caaattttat gatttataat cagtcaatgg cgggaaaaat
     1021 taattccgtt aaaaattttt tgctggcggt ttt
//
LOCUS       DAOQWE010000271         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_194192_length_1050_cov_7.075377, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000271 DAOQWE010000000
VERSION     DAOQWE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194192_length_1050_cov_7.075377"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..>1050)
                     /locus_tag="PKK37_05285"
     CDS             complement(151..>1050)
                     /locus_tag="PKK37_05285"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNR81824.1"
                     /translation="VLSVIQRDKAAAREYLKGQKDVLEQKIAVAVAALDDTVRKKEAL
                     TAEIIGLAGSENEKNTLKENIAAMEREIAARETEIIAGRKILLADTDGDGLLDGREME
                     LGTDPLNPDTDGDGLLDGDEAAMGYDPLTPSEFAVDSAVDPRPLPPMQFEIYKVEEAV
                     SVKIGEDGRSAIRFSGQGLPLSYVTLFIYSAPVIVAVKTDAHGRWTYTLDKPLEDGQH
                     SVYAARVNAQGRIAARSEVYVFKKTGDQIEKLVAGQEASMSSATGRIIQDFKFVIIII
                     VVITVAVALAAVGYLANRGAKKV"
BASE COUNT          240 a          326 c          241 g          243 t
ORIGIN      
        1 cattctcaaa cgattgcacc gtcaagagaa cgtaaaaata aagcgccagc ggaataacga
       61 cgatcagcgc caaagaaaac aaaatcgccg gattttcgcg cacgaaaaca aacgtatttt
      121 gaaaatatcg cttcatttaa tttttccgcg tcaaactttc ttcgcgccgc ggttcgccag
      181 ataaccaacc gccgccagcg ccaccgccac ggtaatcacc acaatgatga tgatcacaaa
      241 cttgaaatcc tggattattc tgccggtcgc cgatgacatt gatgcttcct gcccggccac
      301 caatttttca atttgatcac cggttttttt gaaaacataa acttcggaac gcgcggcgat
      361 tctcccctga gcgttgaccc tggcggcata gacagagtgc tggccgtcct ccagcggctt
      421 gtccagagtg tatgtccacc ttccgtgcgc gtccgttttc accgccacga tcaccggcgc
      481 ggaatatatg aaaagcgtaa cataggagag cggcaagcct tgccccgaaa aacggattgc
      541 cgaccggcca tcttcgccaa ttttcaccga aaccgcttcc tcaactttgt agatttcaaa
      601 ctgcatcggc ggcaaaggcc gcggatcaac cgccgaatca accgcaaatt cgctgggggt
      661 aagcggatca taacccatcg ccgcctcatc gccgtccagc aaaccgtcgc cgtcggtgtc
      721 gggattcaac gggtctgtcc ccagctccat ttcccggccg tccagcaaac cgtcgccgtc
      781 ggtgtcggcc agcaatattt tccggccggc gatgatttcc gtctcgcgcg cggcaatctc
      841 ccgttccatc gccgcaatgt tttctttgag cgtgtttttt tcattctcac tgccggccaa
      901 accaataatc tcggcggtca acgcttcttt tttccgcacc gtatcgtcca gggcggccac
      961 cgccacggca attttttgct ccaaaacatc tttctggcct ttgaggtatt cgcgggcggc
     1021 ggccttgtcg cgctggataa cagacagcac
//
LOCUS       DAOQWE010000272         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_195194_length_1047_cov_4.097782, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000272 DAOQWE010000000
VERSION     DAOQWE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195194_length_1047_cov_4.097782"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PKK37_05290"
     CDS             <1..277
                     /locus_tag="PKK37_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNR81825.1"
                     /translation="LVCELERETTAEEINSILEKATGKKPLKGIMAYEAAPLVSSDYI
                     ASGYSSIVDAGLTMAKGNLVKIGAWYDNEWGYSTRLAEFAEFIGNKL"
     gene            326..>1047
                     /locus_tag="PKK37_05295"
     CDS             326..>1047
                     /locus_tag="PKK37_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNR81826.1"
                     /translation="MKKVGELYWPEAGGKKRAWLNSKSIYAAAEKNPVKFWGNIAKEL
                     VWRKKWKKIYVDTPPRARWFSGAKLNITENCLDRHLAARKNKAAIIWEADDPARPARI
                     LTYYDLYRRVNKFANALKKLGVKKGDRVGIYLPMIPEVIVAMLACARIGAVHSVVFSA
                     FSAAALEARLRDAGAEILITADGYFRRGKIVELKKQADEAARDAAIKKTVVVRHADNE
                     IPWEGERDVWYERITAGESDECA"
BASE COUNT          270 a          240 c          314 g          223 t
ORIGIN      
        1 tttggtatgc gagttggagc gcgaaaccac cgctgaagaa atcaattcaa ttttggaaaa
       61 agccaccgga aaaaaaccgc tcaaaggtat tatggcctat gaagccgcgc cgttggtttc
      121 cagcgactat atcgccagcg gctattcgtc aatcgtggac gcgggcttga ctatggcaaa
      181 gggtaatctg gtgaaaatcg gcgcatggta tgacaacgaa tggggctatt ccacccgcct
      241 ggccgaattt gccgaattca tcggcaacaa gttataaatt cggatattcc attaagtcaa
      301 attttgaggc aataatcaga aatctatgaa aaaagtcgga gagttgtatt ggccggaggc
      361 gggagggaaa aaacgggcgt ggctcaattc aaaaagtatt tatgccgcgg ccgagaaaaa
      421 tccggtgaaa ttttggggga acatcgccaa ggagctggtt tggcggaaaa aatggaaaaa
      481 aatttatgtt gatacgccgc cgcgcgcgcg ctggttttcg ggcgcaaaat tgaacatcac
      541 cgaaaattgc cttgatcggc atttggcggc gcgcaaaaac aaagccgcga tcatttggga
      601 agctgacgat cccgcccgtc cggcgcggat tttaacctat tatgatcttt accgccgggt
      661 gaacaagttt gccaacgcgc tcaaaaaatt gggcgttaaa aagggcgacc gggtgggaat
      721 ctatctgccg atgatccccg aagtcatcgt ggcgatgctg gcgtgcgccc ggatcggcgc
      781 ggtgcattcg gtggtgttct ccgcgttttc ggcggcggcc ttggaggccc gtcttcggga
      841 cgcgggcgcg gaaattttaa ttaccgccga cggctatttc cgccgcggca agatcgttga
      901 gctgaaaaag caggccgacg aagcggcacg tgacgccgcg attaaaaaaa cggtggttgt
      961 ccggcatgcc gacaacgaaa ttccctggga gggcgaacgg gatgtttggt atgagcggat
     1021 cacggcgggt gaatccgacg agtgcgc
//
LOCUS       DAOQWE010000273         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_195734_length_1046_cov_1.892028, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000273 DAOQWE010000000
VERSION     DAOQWE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195734_length_1046_cov_1.892028"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKK37_05300"
     CDS             complement(<1..262)
                     /locus_tag="PKK37_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81827.1"
                     /translation="MANIAKKRGRPKLIKDELRAVWIDRNKRRQQDNLSYMVYIYSLL
                     YPDFEEKKLESEPAEPDFVFLWDLKSDKMKTTILAELGRLAVR"
     gene            complement(548..721)
                     /locus_tag="PKK37_05305"
     CDS             complement(548..721)
                     /locus_tag="PKK37_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81828.1"
                     /translation="METRKKALTISDAQERELVGMLQDLSENQLAALRDLLKSIINQG
                     EPAPAVEPRIRQL"
     gene            complement(803..>1046)
                     /locus_tag="PKK37_05310"
     CDS             complement(803..>1046)
                     /locus_tag="PKK37_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017304171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNR81829.1"
                     /translation="YEANLEMAWLDGADLMGANFRGAILEQTNLRGVKNIRDAQGFKF
                     VQLKLTNMDRETQEYLATINLTVPRFAPAKKKRALI"
BASE COUNT          219 a          285 c          240 g          302 t
ORIGIN      
        1 aacggaccgc cagccgccca agttcggcca aaatcgtcgt tttcatttta tcggatttga
       61 ggtcccataa gaaaacaaag tccggttctg ctggttcgga ttctaatttt ttttcttcaa
      121 agtccggata taacaagctg taaatataaa ccatatagct caaattatct tgctggcgcc
      181 ttttgtttcg gtcaatccag acggcccgca attcgtcttt aattaatttc ggccgtcccc
      241 gctttttggc aatgtttgcc ataattcgcg ccccggtcaa aagtccagtt catcggccgg
      301 cgccggtttg gccgcggccg ggtccttttc ccgcgctttc agataaatgt aataatccgg
      361 gtcacgttcc gttttcttga atgtattcgg catgataaca aaggacgtga ccgcgttcaa
      421 ggatcccgac agcatggaaa cgcccgcctt tgtctttgat ttccaaagtc cggttaactt
      481 gactaaatcg ctcattttat cgcctcgtta atttagaata acttcgaatt caaaccccgg
      541 cgcccggcta taattgacga atgcggggtt cgacggccgg cgcgggttcg ccctggttaa
      601 taatcgattt caacagatcc cgaagcgccg ccagctggtt ttcgctcaaa tcctgcagca
      661 tcccgacaag ttcgcgctct tgcgcgtccg atatggtcaa agcctttttt ctggtttcca
      721 tgattcccgc cctttccttg aatttcgtta aagtccgccg gcaaatggtc cgccggcggg
      781 ttattagatc ccggccgcgc ggttaaatca atgcccgctt tttcttcgct ggcgcgaacc
      841 gcggcaccgt taaattgata gttgcaaggt attcctgggt ttcgcggtcc atgttggtta
      901 atttcaattg aacgaatttg aacccctgcg cgtcccgaat gtttttgacg cccctcaaat
      961 tagtttgttc aagtattgcg ccgcggaagt tcgcgcccat caggtccgcg ccgtcaagcc
     1021 aggccatttc aaggtttgcc tcgtat
//
LOCUS       DAOQWE010000274         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_196941_length_1042_cov_2.797366, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000274 DAOQWE010000000
VERSION     DAOQWE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196941_length_1042_cov_2.797366"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PKK37_05315"
     CDS             <1..663
                     /locus_tag="PKK37_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81830.1"
                     /translation="VTVLKDGQIATAVKADANANFRVEITDITAGVWTFGLWSEDKNG
                     NRSITFSFTANVNANMTTTINGIFLPPTIQLDKTNVKKGEPLGILGQTAPKSEIQITI
                     NSGEPIVIEKIKPDAQGAWFHVFDTSPLEYGSHTTRAKATANDGAASGYSNMVSFSVG
                     STSFYCSKNPDLNNDKRVNLVDFSILLYDWGTPKNKVADINCDGKTNLTDFSIMMYYW
                     TG"
     gene            722..>1042
                     /locus_tag="PKK37_05320"
     CDS             722..>1042
                     /locus_tag="PKK37_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81831.1"
                     /translation="MKKTRCPLSCILYFLFAVHYLLFAAPATAQEEARLYVEPAQIAA
                     KTEEIFVAKIRLAPGGECVNAAEIILSFDQKSLEIVDFSRGESIISLWTDSPSREKIA
                     LANES"
BASE COUNT          292 a          251 c          234 g          265 t
ORIGIN      
        1 gtcacggttt taaaagacgg ccagatcgcc accgcggtca aagccgacgc caatgccaat
       61 ttccgggtcg agatcactga tattaccgcc ggcgtgtgga ctttcggact ttggtcggaa
      121 gataaaaacg gaaacagatc gataactttc agcttcaccg ccaatgtcaa cgcgaatatg
      181 accaccacca tcaacggcat ttttttgccg cccacaatcc aattggataa aaccaatgtc
      241 aaaaaagggg aaccgctggg tattttggga caaaccgcgc ccaaatccga gatccagata
      301 accatcaact ccggcgaacc gatcgtcatt gaaaaaatca agcccgacgc gcaaggcgct
      361 tggttccatg ttttcgatac ttctccgctg gaatacggct cccacaccac tcgcgccaaa
      421 gccaccgcca atgacggcgc cgccagcggc tattccaata tggtttcttt ttcggtggga
      481 agcaccagtt tttactgttc caaaaacccg gacttgaaca acgacaaaag ggtcaatttg
      541 gtggattttt caattctttt gtacgattgg ggaacgccca aaaataaagt tgccgacatt
      601 aattgcgacg gcaaaaccaa tctgaccgat ttttccatta tgatgtatta ttggacggga
      661 tagaaataat aatcagtaat tagcgattat atatgaaaat gttaaataaa tttttaagca
      721 aatgaaaaag acccgttgtc cgttatcttg tattttatat tttttgtttg ccgttcacta
      781 tttgctgttt gccgctcccg caacggcgca agaagaggcg cggctctatg ttgaaccggc
      841 gcaaatcgct gcgaaaacgg aggaaatttt tgttgccaag atccggttag cccccggcgg
      901 cgaatgcgtc aacgccgcgg agattatttt gtcttttgat caaaaaagtt tggaaatcgt
      961 cgatttttcg cgcggggaat cgataatttc tttatggacg gattcgcctt cacgcgaaaa
     1021 gatcgcgctt gccaacgaaa gc
//
LOCUS       DAOQWE010000275         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_197616_length_1040_cov_2.731980, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000275 DAOQWE010000000
VERSION     DAOQWE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197616_length_1040_cov_2.731980"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1040
                     /locus_tag="PKK37_05325"
     CDS             <1..>1040
                     /locus_tag="PKK37_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNR81832.1"
                     /translation="DAEIARPMVTKMKQILSHLINIGVGYLSLDRPVSTLSGGESQRV
                     KMARQLDCGLVGLMYILDEPSIGLHPRDNHKLIEILKELRDKGNSVFVVEHDPDIIRC
                     ADYIIDVGPRAGANGGEIIYSGPFAGLTQSKGLTAEFLNNRSPVAKARKPWTEYIEIK
                     NASLNNLKNISTKIPKGVLTCVTGVAGSGKSSLINDVFLSQNRGAIVIDQSPIGKSSR
                     SNPATYVGIFDEVRKEIAAKTRASASLFSFNSAGACPKCKGLGTISYEMNFLDEVRVV
                     CDECQGKRYTEKVLAIRYNGKNIDQILHTTIAGLKGFFQDSQINKKLQVLTDVGLDYL
                     ELGQPLSTLSGG"
BASE COUNT          273 a          281 c          262 g          224 t
ORIGIN      
        1 tgatgccgag attgcccgcc cgatggtaac aaaaatgaaa caaatccttt cgcatttgat
       61 caatatcggc gtcggctatt tatcgctgga ccgaccggta tcaacgcttt ccggcggcga
      121 aagccagcgg gtgaaaatgg cgcgccagct tgattgcggc ctggtcggct tgatgtatat
      181 tttggacgaa ccgtccatcg gcctccatcc gcgcgacaat cacaagctga ttgaaatttt
      241 gaaagaactg cgcgacaagg gcaacagcgt ttttgtggtt gagcatgatc ccgacataat
      301 ccgttgcgcc gattacataa ttgatgttgg tccccgcgcc ggcgcaaacg gcggcgagat
      361 catttattcc ggcccgtttg cgggattgac gcaatcaaaa gggctcaccg ccgaattctt
      421 gaacaaccgc tcccccgttg ccaaggcaag aaaaccctgg accgaatata ttgaaataaa
      481 aaacgcctcc cttaacaatt tgaaaaatat atcaaccaag attcccaaag gggtgctgac
      541 ctgcgtcacc ggcgtcgccg gttcgggcaa gagcagtttg atcaacgatg tttttctaag
      601 ccagaaccgg ggcgccatcg tcattgacca gtcgcccatc ggcaaatcgt cgcgctccaa
      661 tccggcaacc tatgtcggca tcttcgacga agtgcgcaag gaaatcgcgg ccaaaacccg
      721 cgccagcgct tccctgttca gttttaattc cgcgggcgcc tgcccgaaat gcaaggggct
      781 gggaactata agttacgaaa tgaacttttt ggacgaggtc cgggtcgtct gcgacgaatg
      841 ccaaggcaaa agatacaccg aaaaggtgct ggccatccga tacaacggca aaaatattga
      901 ccaaatcctc cacaccacca tcgcggggct aaaaggattc tttcaagatt cgcaaatcaa
      961 taaaaagctt caggtattaa ccgatgtcgg cctcgattat ctggaattgg gccagccgtt
     1021 aagcacgctt tcgggcggcg
//
LOCUS       DAOQWE010000276         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_197876_length_1039_cov_8.279472, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000276 DAOQWE010000000
VERSION     DAOQWE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197876_length_1039_cov_8.279472"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..371
                     /locus_tag="PKK37_05330"
     CDS             174..371
                     /locus_tag="PKK37_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNR81833.1"
                     /translation="MNGTIKTLTERGFGFISREGEAKDLFFHSKELRGVEYADLKVGD
                     AVTFEVVDGEKGPAAVNVARA"
     gene            complement(502..738)
                     /locus_tag="PKK37_05335"
     CDS             complement(502..738)
                     /locus_tag="PKK37_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HNR81834.1"
                     /translation="MDNLALFFLFVLIAIIITYFFPAIVAVARRKRNWGAILALNLLA
                     GWTFVGWVAALVWALTHDCGENGKKPAPPAAIDG"
BASE COUNT          286 a          226 c          257 g          270 t
ORIGIN      
        1 gatgaagccg cggttttggc ggttaaagaa ttaaatccgg gcgttgaggt tgcgtgttcc
       61 gacgcccggc ggggactgaa atttggagaa aaaggggtta tggtgtatag tgttaagaca
      121 gttagaaaac ggaaaatggt cggtttcccg ttgtgcgtaa attcaacaaa aaaatgaacg
      181 gaacaatcaa aactctcacg gaaagaggat ttggattcat ttcccgcgaa ggggaagcca
      241 aagacttgtt tttccattcc aaagaattgc gaggcgtgga atatgccgat ctcaaagtcg
      301 gcgatgcggt gacatttgaa gttgttgacg gtgaaaaggg accggcggcg gtcaatgtgg
      361 cgcgcgccta gtccaaaaaa taaaacgtcc cgaaactgtc ggggcgtttt gtttttttct
      421 gatatcgtag cggtatttga taccgctact ctcagggttt tttgtttgat atttgccggc
      481 aatgtcgggc atcacgccaa atcagccgtc aatggcggcc ggcggcgcgg gttttttgcc
      541 gttttccccg caatcgtgcg tcaacgccca aaccagcgcc gcgacccagc cgacaaacgt
      601 ccatcccgcc agcaaattca gagccaaaat cgcgccccaa tttctttttc tgcgggcaac
      661 ggccacaatg gcgggaaaaa aataggtgat gattatcgcg atcaaaacaa aaagaaagaa
      721 aagcgccaga ttgtccataa ttttgatatg ttaatagatt ttgatttcgc tttatgccat
      781 tttatcacat tttgatccgc gatcacataa tctttcagtg gttttggcca acgccgcggc
      841 aaaaatccgt ttttttcgtt tttgtgatat aatgggcgat aaaagaataa tcaaatcaaa
      901 aaatatgaat atgaaaaaga tcgtttatgc cgttgtcgcc gttgtttgtg tcgccgcact
      961 cgttttcttg gcctatcgtt tcgcatcaac gcccgaagaa actcaaaatc cgatgccggc
     1021 cgaagaatcg caaacccaa
//
LOCUS       DAOQWE010000277         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_199196_length_1035_cov_4.820408, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000277 DAOQWE010000000
VERSION     DAOQWE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199196_length_1035_cov_4.820408"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..>1035
                     /locus_tag="PKK37_05340"
     CDS             422..>1035
                     /locus_tag="PKK37_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81835.1"
                     /translation="MKKIKRKSLVFAGVVMLVAGFFSALLAFAGTSDNVGGFAWSENI
                     GWVSFNNKAVDGIAGDGGATDYGVNIATDGTMSGYAWSENIGWISFNGADAAACGGCS
                     GDDCQAKVNQTLNGSGEYEVGGWAKVLANGGGWDGCISLRGSNYGVSIDPATGDFHGW
                     AWSDMVLGWLSFNSVDSGAGGGPYKVHTTAGFGPTAKMECGGAGA"
BASE COUNT          225 a          213 c          330 g          267 t
ORIGIN      
        1 acggcatcat tgacgaagtg atcaaagcca agggttgaga gcataaagcg caaagtaaaa
       61 agcgtaaagg ggcctcgcaa gagcggggct ttttgctatt gcttttggta accactcact
      121 ggggcctgta ttttaagggt ctatccctcg cgcttgtccc gaggtaatcc gagggataat
      181 cgcgagcggt tagaccctgg caaaagaatt ttgggcgcga aattaaaggg gactaaccct
      241 cgcagtaacg agggatagac ccctttaatt ttaaacctgg tgagtggtta ctctttttgg
      301 tattgcgcgc caaaaataat gtgttatgat ggggttgtga gaaagttttt aaggttaaat
      361 cggtaatttt ccgccgccga aagccgtttt ggcaacacgg ccaaggcgcg gttccaatgc
      421 cttgaaaaaa ataaaacgca aatctcttgt ttttgccggc gttgttatgc tggtggcggg
      481 ttttttttcc gcccttttgg catttgcggg aacgagcgac aatgtcggcg ggtttgcctg
      541 gtcggagaac atcggttggg tgagtttcaa taacaaagcg gttgacggta tcgccggcga
      601 cggtggtgcc acggattacg gcgttaatat tgctactgac ggcacaatgt cgggctatgc
      661 ctggtcggag aacatcggct ggatttcatt taatggcgcg gatgcggcgg cctgcggcgg
      721 ctgttccggc gatgattgcc aggccaaggt gaatcaaacg cttaacggtt cgggggagta
      781 tgaagttggc ggttgggcga aagttttggc caatggcggc ggttgggatg gttgcataag
      841 tttgcgcggt agcaattacg gcgtttccat tgatccggcg acgggtgatt ttcacggctg
      901 ggcgtggtcg gatatggttt tgggctggtt gagtttcaac agtgttgatt ccggtgcggg
      961 cggcggaccg tataaagtcc ataccactgc cggttttggt ccgaccgcca aaatggaatg
     1021 cggcggcgcg ggcgc
//
LOCUS       DAOQWE010000278         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_200170_length_1032_cov_7.210850, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000278 DAOQWE010000000
VERSION     DAOQWE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200170_length_1032_cov_7.210850"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1032)
                     /locus_tag="PKK37_05345"
     CDS             complement(<1..>1032)
                     /locus_tag="PKK37_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNR81836.1"
                     /translation="EDGRGKAVITGTGDKTEIGKINCLVRETKEDKTPYQKKLAHFSK
                     IVGSIIGTICIGIFAEGMLTGGEFVEMFTTAVAIAVASIPEGLPVAMTVILALGMQRI
                     FKKGGLVRKLAAAETLGSTSIIATDKTGTLTKGRMNVAGIFTFSLPEEKVLKTAILCS
                     EAFVENFWDPPEKWKVRGMPTERALILEGAKFGLVKNKLENAEPKIGEISFSSASKYS
                     ASLRRPAAAVSRREFLPGGEEKNIIYAKGAPEVILEKSKYLEFNGRRTEMDKEKRELL
                     HKKCEELTGKGYRVLAAAYREVRAAEVGADDLKKSVADLVFIGLVFLEDSVREEAKEA
                     IQTCYRAGM"
BASE COUNT          216 a          314 c          221 g          281 t
ORIGIN      
        1 ctcattcccg cccgataaca ggtttgtatc gcttccttgg cttcctcgcg caccgaatct
       61 tccaaaaaaa ccaacccgat aaaaaccaga tcggcaaccg atttttttaa atcgtcggca
      121 ccaacctccg ccgcgcggac ttcccgatag gccgcggcca aaacccggta tcccttgccg
      181 gtgagctctt cgcatttttt atgcaaaagc tccctttttt ctttgtccat ctccgtccgc
      241 cggccgttga attccagata ttttgatttc tccaaaatca cctcgggggc acctttggcg
      301 taaatgatgt ttttttcttc gccgccgggc aaaaattccc ggcgagaaac cgcggcggcc
      361 ggccgcctca agctggccga atatttggac gcggaagaaa aggaaatctc gccgattttg
      421 ggttcggcgt tttccagttt gtttttaacg aggccgaatt tggcgccttc caaaatcaac
      481 gccctttccg tgggcattcc ccttactttc catttttccg gtggatccca aaaattttca
      541 acaaacgcct cactgcacaa gatggcggtt ttcaaaactt tttcctccgg cagggaaaag
      601 gtgaagattc cggcgacgtt catccggcct ttcgtgagcg tgccggtctt gtcggtggca
      661 atgattgaag tgcttcccaa agtttcagcc gcggccagtt tccgcaccag cccccctttc
      721 ttgaaaatcc tctgcattcc caacgccaaa atcaccgtca tggccaccgg caaaccttcg
      781 ggaatggacg ccaccgcaat cgccaccgcc gtggtgaaca tttccacgaa ttcgccgccg
      841 gtgagcatcc cttccgcaaa tatgccaatg cagatcgttc cgataatact gccgacaatt
      901 tttgagaaat gggccagttt tttttgatag ggagttttgt cttctttggt ttctctcacc
      961 agacaattaa tctttccgat ttcggtttta tctcccgtgc ccgttatcac cgccttgccc
     1021 cgcccgtctt cc
//
LOCUS       DAOQWE010000279         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_200453_length_1032_cov_1.587513, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000279 DAOQWE010000000
VERSION     DAOQWE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200453_length_1032_cov_1.587513"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKK37_05350"
     CDS             <1..344
                     /locus_tag="PKK37_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81837.1"
                     /translation="NKEGDELSPRDIIALTNANIAVDDDIDVVLDYAKFKKISVAEAL
                     KSSVVKATLAENEEHRKTAAATNTGAGRRGVQQKSEDSLLSEFKSGKVPETDDEMDRL
                     ALARMKARAGK"
     gene            397..>1032
                     /locus_tag="PKK37_05355"
     CDS             397..>1032
                     /locus_tag="PKK37_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81838.1"
                     /translation="MGNTKGSQTWREKYRSSSFQTYLKNAIVAPAICQVDTSDNYLIK
                     DPYSATPTVTVQALAGTYSVADWTTTNDYLTVTDEFIVGEHVFDFETLLGQFDMMADR
                     IDRQTYALAAAVDKWVLNNLCEDGDGSYTTPTGGFTTAANVLTILANLNSKWAGYANA
                     FQTPFLVIENTDLVGVQIAAMTNGFSTADAALKNGKITSLAGIDIYVVPSGT"
BASE COUNT          290 a          223 c          271 g          248 t
ORIGIN      
        1 aaaacaaaga aggtgatgag ttatctccac gtgatattat cgcattaacg aacgcgaata
       61 tcgctgttga tgatgatatt gatgttgttc ttgattatgc gaaatttaaa aagatttccg
      121 ttgcggaagc cctaaaatca agcgtggtca aggcaacgct ggctgaaaat gaagagcacc
      181 gcaaaaccgc cgccgcgaca aataccggcg cgggaagacg cggagttcaa cagaaaagcg
      241 aagactctct gttgtcggaa ttcaaatcag ggaaagtccc cgaaacggat gacgaaatgg
      301 atcgtctcgc gctggccaga atgaaagcaa gagcgggtaa atagctgtgg ggtatttacc
      361 aaatctgtgg gtgtaaattg ttaaataaaa cagattatgg gaaatacaaa aggatcgcaa
      421 acttggcgag agaaatatcg ttcttcatcg tttcagactt atttgaagaa cgcgattgtc
      481 gcgccggcta tttgtcaagt ggataccagc gacaattatc taatcaaaga cccttacagc
      541 gccacgccga cggtaacggt tcaggccttg gccgggactt acagcgtcgc tgattggacg
      601 accaccaacg attacctgac ggtgaccgat gaatttattg ttggcgaaca cgtctttgat
      661 tttgaaacgc tgttgggtca gtttgatatg atggctgacc ggattgaccg ccagacttat
      721 gctttggccg cggcagtgga taaatgggtg ttaaacaacc tttgcgaaga cggcgatggt
      781 tcttacacca ccccgaccgg cgggttcacc actgcggcga acgtgttgac cattcttgcc
      841 aatttgaatt ccaaatgggc gggatatgca aacgcattcc aaacaccttt cttggtgatt
      901 gaaaacactg acttggtggg cgtgcagatt gcggcgatga ccaacggctt ttcaaccgcg
      961 gacgcggcat tgaaaaacgg caaaattact tccttggcgg ggattgatat ttatgtcgtg
     1021 ccgtccggaa ct
//
LOCUS       DAOQWE010000280         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_201859_length_1027_cov_7.016461, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000280 DAOQWE010000000
VERSION     DAOQWE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201859_length_1027_cov_7.016461"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1027)
                     /locus_tag="PKK37_05360"
     CDS             complement(<1..>1027)
                     /locus_tag="PKK37_05360"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR81839.1"
                     /translation="QCVTGDTLLRVRRRRKRQNERGEWVEEEYFEDCRIDQIKAGDEI
                     LTLDENTGRLTVSRVNALMDMGVKEIYKLTTATGKTIRTTANHPYLAVDGFDNERAAV
                     FVDSANFEMSCRFCGLEKDYVKMDKFFFGAMKKSAKTFRFYKVDFGGNKQRKFFDFLN
                     IIGAKIVSKPIKVIKQKQKANEHKANFDVELAVDAVELKEKYDTAIIVSGDSDFAYLA
                     EHLQKAGKKTVVISPWRRTGKELRQQADMYFDLRNMPFVGRIKIKRPPVGGINPGFSP
                     ALPILSQVHPFVKGAGRWTKVSQLREGQMIAVAGDNNRPQYERIAKIEKLPAEQVYDI
                     EVEGTHNF"
BASE COUNT          224 a          287 c          231 g          285 t
ORIGIN      
        1 cgaaattgtg ggtgccttcc acttcaatgt catagacctg ctcggcgggc agtttctcaa
       61 ttttggcgat gcgttcatat tgcggccggt tgttgtcgcc ggcaaccgcg atcatctggc
      121 cttcgcgcag ttggctcact ttggtccacc ggccggcgcc cttgacaaac ggatgcacct
      181 gcgataaaat cggaagtgcc ggcgagaagc cggggttgat cccccctacg gggggtcttt
      241 taattttgat gcgcccgaca aaaggcatat tcctcaaatc aaaatacata tccgcctgtt
      301 gtctcaattc tttgccggtc cttctccaag gagaaatcac gaccgttttc tttcccgcct
      361 tttgcaaatg ttctgccaaa tacgcaaaat ccgaatcgcc ggaaacgatt attgcagtat
      421 catatttttc tttcaattca acggcgtcca ccgcgagctc aacatcaaaa ttagccttgt
      481 gttcgttggc cttttgtttt tgtttgatca cttttatcgg cttgctcacg atcttggcgc
      541 cgataatatt caaaaagtca aagaatttgc gttgtttgtt cccgccgaaa tcaaccttgt
      601 aaaaacggaa tgttttggcg ctttttttca ttgcaccaaa gaagaattta tccattttca
      661 catagtcctt ttccaaaccg caaaaacggc agctcatttc aaaatttgcc gaatcaacaa
      721 acaccgccgc gcgctcgttg tcaaacccgt ccaccgccag atagggatgg ttggcggtgg
      781 tgcggatggt tttgccggtg gcggtggtga gcttgtaaat ttctttgacg cccatatcca
      841 tcagcgcgtt gacgcgcgac accgtcagcc ggccggtgtt ttcgtccaac gtcaaaatct
      901 catcgccggc cttgatctga tcaatgcggc aatcttcaaa atattcttcc tccacccatt
      961 cgccccgctc attctgccgc ttgcgccgcc gccgcacgcg caaaagcgtg tcgccggtaa
     1021 cgcactg
//
LOCUS       DAOQWE010000281         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_202130_length_1027_cov_1.548354, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000281 DAOQWE010000000
VERSION     DAOQWE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202130_length_1027_cov_1.548354"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="PKK37_05365"
     CDS             complement(<1..108)
                     /locus_tag="PKK37_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNR81840.1"
                     /translation="MLDLIIIGGGPAGMAAAIYAARQKTDFLLLTKEFGG"
     gene            complement(223..>1027)
                     /locus_tag="PKK37_05370"
     CDS             complement(223..>1027)
                     /locus_tag="PKK37_05370"
                     /inference="COORDINATES: protein motif:HMM:NF023746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81841.1"
                     /translation="INIDFADLRAILVGGGKMAFLNTVSLKKGEEAGAEVFERVFSSP
                     LYPYGIDKARRLLLNIVAQRDLKLAEVNRILTGAARRIHKDARIIFGVGHEGARENAI
                     KVSALATGCVYAGMNNGDDNNGGRQADTPPAPKRPLPPPAPSKAAPKKIVKKAAKQTS
                     KKTEIKKTPAKSKKNIKSAPAKPVKRIAVKIKTPVKAKEAPESLVETKIAPEGYQNEQ
                     SIGAQFEAPERKNALQVKKEIELEEQKMLEQEKAWEVPAFLRKQKKNQN"
BASE COUNT          223 a          262 c          199 g          343 t
ORIGIN      
        1 gccgccaaat tccttggtga gcaataaaaa gtcggtcttt tggcgcgccg cgtaaattgc
       61 cgccgccatt cccgccggtc cgcccccgat aataattaaa tccaacataa aaatttaaaa
      121 ttgaaatatc aaaattaaaa attacaatgc aaaatttaaa attggtaaat tttaaaatta
      181 tttaatttta cattttaaac tttcattttg atttttgatt tttcaatttt gatttttttt
      241 ctgtttgcgt agaaacgcgg gcacttccca tgctttttcc tgttccagca ttttttgttc
      301 ctccaattca atttcttttt tcacctgcaa cgcgtttttt ctttccggcg cttcaaattg
      361 cgcgccgatg ctttgttcgt tttggtatcc ctcgggcgcg atttttgttt caaccaaaga
      421 ttccggcgct tcttttgctt taaccggcgt tttaattttc accgcgattc ttttgactgg
      481 ttttgccggc gcggatttaa tgtttttttt gctttttgcc ggcgtttttt taatttcggt
      541 tttttttgaa gtttgttttg ccgctttttt cacgatcttt tttggcgcag ctttgctcgg
      601 cgcgggcggt ggcaacggcc gcttgggcgc gggcggcgtg tcagcttgac gaccgccgtt
      661 attgtcatcg ccattattca ttcccgcgta aacgcaaccg gttgccagcg ccgaaacttt
      721 aattgcgttc tcgcgcgcgc cttcatgccc cacgccgaaa attatccgcg cgtccttatg
      781 aatgcgccgc gccgcgccgg tcaaaatgcg attgacttcc gccaatttca aatctctttg
      841 cgccacaata ttgagcaaaa gccttctggc cttgtcaatg ccataaggat aaagcggcga
      901 agaaaacact cgttcaaaca cctccgcgcc tgcttcctcg ccttttttca aagacacggt
      961 gtttaaaaac gccatcttgc cgccgcccac caaaatcgcc cgcaaatcgg caaaatcaat
     1021 attaatc
//
LOCUS       DAOQWE010000282         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_204680_length_1019_cov_4.788382, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000282 DAOQWE010000000
VERSION     DAOQWE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204680_length_1019_cov_4.788382"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKK37_05375"
     CDS             <1..990
                     /locus_tag="PKK37_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81842.1"
                     /translation="YPEREIQTKEIQTIHTKEVQIKTETIVVDGGTKDTVEHISRQLD
                     SDRPNYSLGQSFSLPSSLDAKTLRIGDSSGVDFTVDRDGNAGTNNLNVAGDAVIQGNL
                     TVAGAQTYSGAARFEVSTDSPALAAIQNGAGAAFQADDILLKDYKIDFSGLGSLEIDS
                     NGYLILASQSGKIKAADGSVFYTAGGHPINAPGEEILRAATPIFGFDIPARTAAGDFV
                     AISKEMVLSGDSFPAAMEGTARVYKLNIVYADDGAASPSAWRIYNITDSAAGAEFAVP
                     AAQTGGLTESATHITAELTLPAAGKKWRVEAMPPAGKTIQIYQISLEAYDRID"
BASE COUNT          248 a          263 c          290 g          218 t
ORIGIN      
        1 tatccggagc gggaaataca aaccaaagag attcaaacca ttcacaccaa agaagtccaa
       61 atcaaaaccg aaaccattgt cgttgacggg ggcaccaaag acaccgtgga gcatatctcg
      121 cgccagttgg attcggatcg ccccaattat tctttgggac agagcttttc tttgccttcc
      181 agccttgacg ccaaaacttt gcgcatcggg gattcttcgg gcgttgattt caccgtggat
      241 cgggacggca acgccggcac caacaatttg aatgtcgccg gcgacgcagt gatacaagga
      301 aatttgaccg tggcgggcgc gcagacatac agcggcgcgg ccagatttga agtttccacc
      361 gacagcccgg cgctggcggc aattcagaac ggcgcgggcg cggcctttca agccgacgat
      421 attttgctca aggattacaa gattgatttt tccggtttgg gatcactgga aattgacagc
      481 aacggctatt tgattttggc gtctcaaagc ggtaaaataa aagcggcgga cggcagtgtt
      541 ttctacaccg ccggcgggca tccgataaac gctcccggcg aggaaatttt gcgtgcggca
      601 acgccgattt tcgggtttga cattccggcg cgcaccgccg ccggcgattt tgtggcgatc
      661 tccaaggaga tggttttgag cggcgattcg tttcccgcgg cgatggaagg caccgcccgt
      721 gtttataaat taaacatcgt ttatgccgat gatggcgccg cctccccgtc tgcgtggcgg
      781 atttacaaca ttactgacag cgcggcgggc gcggagtttg ccgtgccggc ggcgcaaacg
      841 ggcggattaa cggaaagcgc gacacatatc accgcggaat tgaccttgcc ggccgcgggc
      901 aaaaagtggc gggtggaggc gatgccgccg gcgggcaaaa ccattcaaat ttatcagata
      961 tcccttgaag cctacgaccg gattgactga taaacgataa ataataattg ataattaat
//
LOCUS       DAOQWE010000283         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_204706_length_1019_cov_3.334025, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000283 DAOQWE010000000
VERSION     DAOQWE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204706_length_1019_cov_3.334025"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..>1019)
                     /locus_tag="PKK37_05380"
     CDS             complement(172..>1019)
                     /locus_tag="PKK37_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR81843.1"
                     /translation="LWQGKHEPMITKKLFDKCQKVLSDRARPQKRKIKEFAFRGLLFC
                     GECGCAITSETQKGHNYYRCTKKRNKCSQGYIREEILADQINKQIQKVSLPPSWADKM
                     IMELEKDKEQEAKDGAVFAQNLKSQIKELEDKLDTLLDAHLDGTITKEEYTAKKQKIL
                     NQKIEISEKLKDFEQNGNHWLEPAKKFILDSKQAEIIALQENLFEKKDFLKKIGSNRI
                     LRERELIFEPRGAWQILSKSGLSARRARAEALCAEATNAEHTIWLRILNEIRTFFKKN
                     PSSEF"
BASE COUNT          249 a          216 c          190 g          364 t
ORIGIN      
        1 atttgtatac attttagtat actattttta tactttttat acaaatttat ttattatggc
       61 gcgccagttt tcaactggca cgaaattcgg cggcggcgga aagcgagggc gggcaaaaat
      121 tccttccccc agaccccctt cctttttgcc cgcccgagct ttaaaaattc tttaaaattc
      181 agaacttgga ttttttttga aaaaagttcg gatttcgttc agaatacgga gccaaattgt
      241 atgttcggcg tttgttgcct cggcgcaaag cgcctcggca cgcgccctgc gggcgctaag
      301 gcctgatttt gaaaggattt gccacgctcc gcgtggctca aaaatcaatt cccgttcccg
      361 caaaattcgg ttcgagccaa tcttttttag aaaatctttt ttctcaaaaa gattttcctg
      421 caaagcgata atttcggctt gtttactgtc taaaatgaat tttttggcag gttcgagcca
      481 atgattgccg ttttgctcaa aatccttcaa tttttcagaa atctcaatct tttggttgag
      541 gattttttgt tttttagcgg tatattcttc tttggtaatt gttccgtcca aatgagcgtc
      601 aagcaaagta tccaatttat cctcaagctc ttttatttga gattttagat tttgagcaaa
      661 aacggctccg tctttggctt cttgttcttt atctttttcc aattccataa tcattttatc
      721 agcccaagac ggaggcaaag aaactttttg aatttgcttg tttatctgat ccgccaaaat
      781 ttcttcgcga atatagcctt gcgagcattt gttccgtttt ttggtgcaac gataataatt
      841 gtgtcctttt tgcgtttccg aagttatggc gcaaccacac tcaccgcaaa ataataatcc
      901 gcggaaagca aattccttaa tctttctttt ttgcggtcgg gcgcggtcag ataaaacctt
      961 ttggcattta tcaaaaagtt tctttgtaat cattggctcg tgttttcctt gccacaatt
//
LOCUS       DAOQWE010000284         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_205045_length_1018_cov_3.965732, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000284 DAOQWE010000000
VERSION     DAOQWE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205045_length_1018_cov_3.965732"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKK37_05385"
     CDS             complement(<1..468)
                     /locus_tag="PKK37_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR81844.1"
                     /translation="MKIEIKNLACGYNTGIIAENINLRIESGQILCLLGPNGAGKTTL
                     LKTILGFIKPKRGDILIGGKNINCLSAKKISQSIAYIPQIHTPPFPFSVLDVVTMGRN
                     VHLGMFASPSKKDYALAEETLASLGISFLRDRIYTEISGGERQMVLVARALAQQ"
     gene            complement(480..>1018)
                     /locus_tag="PKK37_05390"
     CDS             complement(480..>1018)
                     /locus_tag="PKK37_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNR81845.1"
                     /translation="SSLFSSLISIAKYAADTDDKLPAITFWLMGSLSSASIGDAKILA
                     LALIIGAIPIFLLRWKLNIMSFGDEEARNMGVDVNRIRPLIIAGASLLTAAAVSTGGM
                     IGWVGLIIPHIARFLVGPNYKILLPVSLICGSLFLLAIDDIARNAFAAEIPLGILTSL
                     IGAPFFIFLLWRGKKSWV"
BASE COUNT          273 a          249 c          255 g          241 t
ORIGIN      
        1 ctgttgcgcc agcgcccggg cgaccaaaac catttgcctt tcgccgccgc tgatttcagt
       61 gtatatcctg tccctcaaaa aagatattcc cagagatgcc aaggtttctt ccgccagagc
      121 gtaatctttc tttgacgggg aagcaaacat tcccagatgg acattgcgcc ccattgtcac
      181 cacgtccaaa accgaaaacg gaaacggcgg agtgtgtatt tgcggaatat aggcgatgct
      241 ttgggaaatt ttttttgcgg acaggcaatt tatatttttg ccgccaatca aaatatctcc
      301 tcgtttcggt ttgatgaacc ccagaatcgt tttcagtaaa gtggttttgc cggcgccatt
      361 ggggcccaaa aggcaaagta tctggccgga ctcaatccga aggttaatat tttcggcgat
      421 gatgccggta ttatagccgc aggcaagatt tttgatctca attttcatat ttcaatatgt
      481 cagacccatg atttttttcc cctccaaaga agaaaaatga aaaacggcgc gccgattaat
      541 gaagtgagta ttcctaaagg gatttcggcc gcaaaagcgt tgcgcgcgat atcgtcaatt
      601 gccagcaaga acaaacttcc gcaaataagg gaaaccggca aaaggatttt gtagtttggc
      661 cccaccagaa accgggcgat gtgaggaatg attagcccca cccagccgat catcccgccg
      721 gttgaaaccg ccgcggcggt gaggaggctg gcgccggcga tgatcagcgg ccggattctg
      781 ttgacatcca cccccatatt gcgggcttct tcgtcgccga aggacattat attgagcttc
      841 cagcgcaaaa ggaaaatcgg aatggcgccg ataatcagtg ccagcgccaa aattttggcg
      901 tcgccgatac tggctgaaga caagcttccc atcaaccaga aagtgattgc cggcaattta
      961 tcgtcggtgt cggcggcgta tttggcaatg gaaatcagcg aagagaaaag cgaagaaa
//
LOCUS       DAOQWE010000285         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_205458_length_1017_cov_2.922037, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000285 DAOQWE010000000
VERSION     DAOQWE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205458_length_1017_cov_2.922037"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1017
                     /locus_tag="PKK37_05395"
     CDS             <1..>1017
                     /locus_tag="PKK37_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81846.1"
                     /translation="PTSDTTTPTNMDANVTFKATATDTNGNDWYLAICKSNAIATSTD
                     GAPTCPGGAWGISNAASSTAEATVAYTITSGEADESYAWYGFACDKTAGNSQCSEMSN
                     TGTSGNNGSPFAVNHRPTFSAVSDEAGNPGASDVTITATTYDDDDDDGSQDTVSLYVC
                     KTAAFNGSSCTGGEWCHSDPAVAPPGALACDLTVPDPQAHGEVNYYPYLVDSHSLQAS
                     GGQHGSTQSYTVNDVVPTISGTPSLNGGGAISLSNEKTTTDIYLTGVVTDDNSCQDIH
                     ASTTADIWLTSVGHTACNQTGEADNNDCYPRVSCSYDAAVEKCTGATDKTGGYKCTIS
                     FQYYA"
BASE COUNT          239 a          335 c          260 g          183 t
ORIGIN      
        1 ccccacttcg gacaccacca cgcccacgaa tatggacgcg aatgtgacct tcaaggccac
       61 cgccaccgat accaacggca acgactggta tttggccatc tgcaaaagca acgcgatcgc
      121 cacttccacc gacggcgcgc ccacctgccc gggcggcgcg tggggaatct ccaacgcggc
      181 cagctccaca gcggaagcca cggttgccta caccataaca tcgggcgagg ccgatgaatc
      241 ctacgcgtgg tatggttttg cttgcgacaa aaccgccggt aattctcaat gttcggaaat
      301 gtccaacacc ggcacttcgg gcaacaacgg ctctcccttc gcggtcaatc accggcccac
      361 ttttagcgcg gtttccgacg aagccggcaa tcccggcgct agcgatgtca ctatcaccgc
      421 cacgacctac gatgacgacg atgacgacgg atcacaagac accgtgagtc tgtatgtttg
      481 caaaaccgcg gccttcaatg gttcatcctg caccggaggc gaatggtgcc atagcgaccc
      541 ggcggtcgcg ccgcccggcg ctttggcttg cgatttgacc gtccccgatc cgcaagcgca
      601 cggggaggtt aattactacc cctatctggt ggacagccat agtttgcaag cttccggcgg
      661 gcaacacggc tcaacgcaaa gctacacggt aaacgatgtg gtgcccacga tttccggcac
      721 gccgtctcta aatggcggcg gggcgatcag tttgagcaat gaaaaaacca ccaccgatat
      781 ttatctcacc ggcgtggtta ccgacgacaa cagttgccaa gacatccacg catcaaccac
      841 cgccgacatc tggctcactt cggtaggcca caccgcttgc aatcaaaccg gcgaagccga
      901 caacaacgat tgctatccgc gcgtgagttg ttcctatgac gccgccgtgg aaaaatgcac
      961 cggcgccacc gataaaaccg gcggctataa gtgcacgatc tccttccaat actatgc
//
LOCUS       DAOQWE010000286         1013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_206835_length_1013_cov_3.147182, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000286 DAOQWE010000000
VERSION     DAOQWE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206835_length_1013_cov_3.147182"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..>1013)
                     /gene="rsgA"
                     /locus_tag="PKK37_05400"
     CDS             complement(51..>1013)
                     /gene="rsgA"
                     /locus_tag="PKK37_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HNR81847.1"
                     /translation="DLGYDEFFEAGRQKMGLGGFEVARVIAEYKELYRVRNAEGEYLA
                     KITGKQIFNAVSREDFPAVGDWVAISKINEEQAIIEGILPRRSMIKRKAAGKNEIQII
                     AANIDVALVTQAVDGDYSLNRFERYFAISRDGGVLPAVVLNKIDLISKEDLDFRIGQI
                     KSRFGDIDIIAASTITEAGLDELRNYIAAGKTYCFLGSSGVGKSTLINKLIGKEAIKT
                     GAVSADTDRGRHTTTGREMYFLGSNSSAPLTMNGIVIDNPGMREVGMADAGAGVENVL
                     AKSPHWPRNANIPTARTPASRGARCWRRLSRASWRLKFTIIMPA"
BASE COUNT          226 a          334 c          186 g          267 t
ORIGIN      
        1 tttttccctt ttttcaaggg cggtcatctc gtaataatca gcctcctttt tcaagccggc
       61 ataattatcg taaatttcag cctccagctt gcccgactta accgccgcca acaccgcgca
      121 ccccggctcg cgggtgtgcg tgcagtcggt atatttgcat tccttggcca atgcggcgat
      181 tttgccaaca cattctcaac ccccgccccc gcatcggcca tgcccacctc tctcatcccc
      241 ggattgtcaa tcacgatacc gttcatagtg agcggagccg aactattgct accaagaaaa
      301 tacatttccc ggccggtggt ggtgtgccga ccccggtccg tatcagcgct caccgcgccg
      361 gtttttatcg cctctttgcc gatcagctta ttgatcaaag tggattttcc cacgcccgag
      421 gatcccaaaa aacaatatgt tttcccggcg gcaatataat tcctcaattc atccagcccc
      481 gcttccgtta tcgtgctggc ggcaataata tcaatatcgc cgaaccgact ttttatttgc
      541 ccgattctga aatccaaatc ttcttttgag atcagatcaa ttttattcaa aacgaccgcc
      601 ggcagaaccc cgccgtcgcg gctgatggca aaataccttt caaaccggtt caaactgtaa
      661 tcgccgtcca ccgcctgcgt caccagcgcg acatcaatat tggccgcgat gatctggatc
      721 tcgtttttcc ccgcggcctt tctcttgatc attgatcttc gcggcaatat cccctcaatt
      781 atcgcctgct cctcattgat tttggaaatc gccacccaat cgcccaccgc cggaaaatct
      841 tcccgggaca ccgcgttaaa aatttgtttg ccagtaattt ttgccaaata ttcaccctcc
      901 gcatttctca cccgatacaa ttctttgtat tcggcgatca cccgcgccac ctcaaaaccg
      961 ccaagcccca tcttctgccg cccagcctca aaaaactcat cataccccaa atc
//
LOCUS       DAOQWE010000287         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_208100_length_1010_cov_2.413613, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000287 DAOQWE010000000
VERSION     DAOQWE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208100_length_1010_cov_2.413613"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..>1010)
                     /locus_tag="PKK37_05405"
     CDS             complement(41..>1010)
                     /locus_tag="PKK37_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR81848.1"
                     /translation="IPIAPGQVYFGQSLKQFVCHSIIILRLSFGQTKGLIKTIADIDV
                     SEGEISSILQEHSKLLKPEFERIKKQIQNEPAVHFDETTYKVFNKNALGNYTWIMASA
                     KTNDAIFACGKNRGQGNAQDLKGSNNPGQIGITDNYAGYKNLFGENHQLCWAHLIRKI
                     RDLAQSDQLPKDKKEYCAGLHQSLKGIFHELNQSLCLPFDLELRQTAKESLLIKLQEF
                     SKPNALDCAKLKTIKETLAANLESYFTCLLYPDVPATNNKAEQLLRHAVIKRKLSFGF
                     KTQKGADAAGVLFSVLLSLWRRSKQNFFAGYRQLLEESRALRALPQ"
BASE COUNT          233 a          211 c          268 g          298 t
ORIGIN      
        1 acattttttg gcatattgca atcgccgcga aggtaaccac tcactggggt agcgcccgca
       61 gggcgcggga ttcttccagc aactgccggt atccggcaaa gaaattctgt ttggagcggc
      121 gccagagcga cagcaatacc gagaacaaaa cgccggccgc gtccgcgcct ttctgggttt
      181 tgaagccaaa ggaaagtttg cgtttgatga cggcgtggcg cagtaattgt tcggccttgt
      241 tgttggtcgc cggaacatcg ggatacaaaa gacaggtaaa atacgattca agattggcgg
      301 ccaaggtttc tttgatggtt ttcagtttgg cgcagtcaag cgcattgggt tttgaaaatt
      361 cctgaagctt gatcagcaaa gattctttgg ccgtctggcg caattccaaa tcgaaaggca
      421 agcaaaggct ttggttcaat tcatggaaaa ttccttttaa tgattgatgc aatccggcgc
      481 agtattcttt tttgtctttt ggcaattgat ccgattgggc cagatcgcgg attttgcgga
      541 tcaaatgcgc ccagcagagc tggtggtttt ctccgaagag attcttgtaa cccgcgtaat
      601 tgtcggtgat gccgatttgg ccggggttgt tgcttccttt caaatcttgg gcgttgcctt
      661 ggccgcgatt tttgccgcaa gcaaatatcg cgtcgttggt tttggcgctg gccatgatcc
      721 aggtatagtt tcccaaagca ttcttattga aaaccttgta ggttgtttca tcaaaatgga
      781 ccgccggttc gttttggatt tgttttttaa tccgttcaaa ttcgggtttc agcaattttg
      841 aatgctcttg caagattgat gaaatttcac cttcggaaac atcaatatcg gcaatggttt
      901 tgattaagcc tttggtttgg ccgaaggata atcgcagaat gatgattgaa tggcaaacga
      961 attgcttgag gctttggccg aaatatactt gcccgggcgc gattggaatc
//
LOCUS       DAOQWE010000288         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_208512_length_1009_cov_2.044025, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000288 DAOQWE010000000
VERSION     DAOQWE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208512_length_1009_cov_2.044025"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..920)
                     /locus_tag="PKK37_05410"
     CDS             complement(159..920)
                     /locus_tag="PKK37_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HNR81849.1"
                     /translation="MNSFKDIAYQILKEAGKPLHSKEITKIALSRGWLKTAGKTPEAT
                     MNAQLVVDINAKKEKSRFIKTAPSTFGLNPEYKVVAETKIEEKIEKIYKVSKDVSTKQ
                     KGDIAEARIAELITLYGDTTLSCYKPISDDEGIDLIVKEKGSLKTMYIQIKSRFGDNP
                     DEIFTATTKASAIADNYSMAVVFCFFDTEEGDLWDYLWFVSAPDLIKLANKLDGGRLL
                     GFVAGRKKKESSKWDNYLIDKRDLANQIIAQMKRI"
BASE COUNT          253 a          223 c          197 g          336 t
ORIGIN      
        1 gcaatttcct tatacaccgc caaattatat caaaagcaca caaaaccact cttatttgta
       61 gtgtttgttg gacgaaatcc gaactttctt tgaacaaaat ccggactgcg aaatttgaac
      121 gccgacggaa tttgtgccag cgttagctgg cgcgccgctc aaattctttt catctgcgca
      181 attatttgat tggctaaatc ccttttatca atcaaataat tgtcccattt gcttgattcc
      241 tttttctttc gtccggcaac aaaaccaagc aaacgcccgc cgtcaagttt attcgcaagt
      301 tttataaggt caggagcgga aacaaaccac aaataatccc aaaggtcgcc ttcctccgta
      361 tcaaaaaagc agaaaacgac agccattgaa tagttatcag ctatggcgga cgcttttgtt
      421 gttgctgtaa aaatttcatc gggattatcg ccaaagcggc ttttgatttg aatatacatt
      481 gttttcaaac tgcctttttc cttcacaatc aagtcaattc cctcatcatc ggaaatcggt
      541 ttatagcagg acaaagtagt gtcgccgtaa agcgtgataa gctcggcgat cctcgcttcg
      601 gcgatgtcgc ctttttgctt ggtggaaaca tcttttgaaa ctttgtatat tttctcaatc
      661 ttctcttcaa ttttcgtttc ggcgacaacc ttatattcag gatttaaccc aaaagttgag
      721 ggggctgttt ttatgaagcg agatttttcc tttttggcat ttatgtcaac gacaagctgg
      781 gcgttcattg tcgcttccgg agttttgccg gcagttttaa gccaaccgcg agaaagcgcg
      841 attttggtaa tctctttgct gtgaagcggt ttgcccgctt ctttcaaaat ttgataggcg
      901 atatccttaa atgagttcat attttacgct tttagcattt tgccgatttc tttttgggtg
      961 gtttccaaaa ttccgcttgc cttgtttttg atttcggcaa tttcggcgc
//
LOCUS       DAOQWE010000289         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_209751_length_1005_cov_3.228421, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000289 DAOQWE010000000
VERSION     DAOQWE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209751_length_1005_cov_3.228421"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKK37_05415"
     CDS             complement(<1..385)
                     /locus_tag="PKK37_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNR81850.1"
                     /translation="MENKTVTPKKRLGQNFLTQAGIARKLIRAAGGGAEETLLEIGPG
                     TGNLTAELLKTGRPVFAVEKDPAMIELLRQRFKDAKKLKLAAGDILEFDETKIPAAYR
                     IVANLPFYAGAPIIRKFLESQNPPRA"
     gene            453..>1005
                     /locus_tag="PKK37_05420"
     CDS             453..>1005
                     /locus_tag="PKK37_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNR81851.1"
                     /translation="MSIPLQLKNFAKLLLAGAVLFAGASALSHQAARSVSAKNQKDAN
                     CVEAHDMFARAAEQARPESPEYLLAGDGCVKAAVPPVAATSRVLAAIVGDFSDSPAPE
                     ETRNAIVEYEVTGDETLAGLAEKFNVNVETIAWANDLSKNASLKTGQKLIIPPVSGVI
                     HYATAGDTLAQIAKKYQAQPEEIV"
BASE COUNT          206 a          267 c          295 g          237 t
ORIGIN      
        1 tcgcgcgggg agggttttgg ctttccaaaa atttgcggat gatgggcgcc ccggcgtaga
       61 acggcagatt ggcaacgatt cggtacgccg ccgggatttt cgtttcgtca aattcaagaa
      121 tgtcgccggc cgccagtttc agttttttgg cgtctttgaa tctttgccgc aaaagctcaa
      181 tcatcgccgg gtccttttcc accgcaaata ccggcctgcc ggttttcagc agttcggcgg
      241 tgaggtttcc cgtgcccggc ccgatctcca acagagtttc ctccgcgccg ccccccgccg
      301 cccggattaa ttttcgggcg atgccggctt gcgtcaaaaa attttgtccc agccgttttt
      361 tcggggttac cgttttgttt tccataattc attgtaaata atcgcggcgt cattgacaaa
      421 tccaagcgat cttatagaat caaaggtgga ttatgagtat ccctcttcaa ctgaaaaatt
      481 ttgccaaact actccttgcg ggagcggttt tgtttgcggg tgcgtccgcg ctttcgcacc
      541 aggcggcgcg ttcggtttcg gccaaaaatc aaaaagacgc gaattgcgtt gaagcgcatg
      601 atatgtttgc ccgcgcggcg gaacaagcgc ggccggagtc gccggaatat cttctggccg
      661 gcgacgggtg cgtgaaggcc gcggtgccgc cggtggcggc cacttcccgg gtgttggcgg
      721 cgattgtcgg cgacttttcg gattcgccgg cgcccgaaga aacgcgcaac gcgattgttg
      781 aatatgaagt caccggcgac gagacgctgg cggggctggc ggaaaaattc aatgttaacg
      841 tggaaactat cgcgtgggcg aatgatttgt ccaaaaatgc gagcctgaaa acggggcaga
      901 aactgattat tcccccggtg agcggcgtga ttcattacgc gaccgccggc gacactttgg
      961 cgcagatcgc caagaaatac caagcccagc cggaggagat tgtcg
//
LOCUS       DAOQWE010000290         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Pacearchaeota archaeon isolate AMR_MDS_1070
            NODE_211145_length_1001_cov_6.110994, whole genome shotgun
            sequence.
ACCESSION   DAOQWE010000290 DAOQWE010000000
VERSION     DAOQWE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852413
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Pacearchaeota archaeon (wastewater metagenome)
  ORGANISM  Candidatus Pacearchaeota archaeon
            Archaea; Candidatus Pacearchaeota.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:18:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,086
            CDSs (total)                      :: 1,060
            Genes (coding)                    :: 1,054
            CDSs (with protein)               :: 1,054
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            tRNAs                             :: 23
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 3 of 6
            Pseudo Genes (incomplete)         :: 1 of 6
            Pseudo Genes (internal stop)      :: 2 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Candidatus Pacearchaeota archaeon"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211145_length_1001_cov_6.110994"
                     /isolate="AMR_MDS_1070"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026773"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PKK37_05425"
     CDS             <1..354
                     /locus_tag="PKK37_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR81852.1"
                     /translation="AIQAFFVQKRFELRSVIATRLNFFQIQRRFVARSPRLRRVSLRK
                     KPCISELNRKVGQIQIQFFGKANGGSACEARNKQSLFPIFSIPPKAEYKASFLIPRFC
                     AVGAMAKGDFINFKE"
     gene            360..>1001
                     /locus_tag="PKK37_05430"
     CDS             360..>1001
                     /locus_tag="PKK37_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNR81853.1"
                     /translation="MKTKFFLYARKSTEDEERQVMSIEAQLAELAEFAKRENIEIAET
                     FIESKSAKKPGREIFNEMMSKVHESKEPIGLIAWHPDRLARNSVDGGQIIYCIDIGKI
                     VSLRFPTFWFEPTPQGLFMLQVAFGQSKYYSDNLSENVKRGIRQKLRRGEWPTLAPFG
                     YVNNPKTRNIEPEPTKSRCVTKAFEEFAQGRHTLESLGQRLSFWGVVSGTGKPL"
BASE COUNT          296 a          213 c          235 g          257 t
ORIGIN      
        1 gctattcaag cattttttgt tcaaaaaagg ttcgagcttc gttcagtaat tgcgaccaga
       61 ttaaactttt ttcaaattca gaggcgattt gttgctcgct cgccccgcct tcggcgggtc
      121 tcgcttcgca aaaagccctg tatttcggaa ttaaatcgta aggtcggaca aattcaaatt
      181 cagtttttcg ggaaagcaaa cggcggttcg gcgtgcgaag cacgcaataa acaaagttta
      241 tttccaattt tttccattcc gccaaaggcg gaatacaaag caagtttttt aatcccgcgc
      301 ttttgcgccg taggcgcaat ggccaaaggc gactttataa attttaagga gtaaacatta
      361 tgaaaacaaa attttttctc tacgcaagaa aatctaccga ggatgaagaa aggcaagtga
      421 tgtctattga agcgcaactc gcggagttgg cggaatttgc caagcgggaa aatatagaaa
      481 ttgccgaaac cttcattgaa agtaaaagcg caaagaaacc cggccgagaa attttcaacg
      541 agatgatgag caaagttcac gagagcaaag agccaattgg cttgattgca tggcacccgg
      601 acagattggc tcgcaattcc gttgacggcg gacaaatcat ttattgcata gatatcggca
      661 agatcgtttc tttgcgcttt cccacatttt ggtttgagcc aactccgcaa ggacttttca
      721 tgcttcaagt cgcctttggc caatcaaaat attattccga caatttgtcg gaaaatgtta
      781 agcgcgggat tagacaaaaa ctcaggcggg gtgaatggcc gacacttgct cctttcggct
      841 atgtgaataa tccgaaaaca agaaacattg aacccgagcc gacaaaatca aggtgcgtta
      901 ccaaagcatt tgaggaattt gcgcaaggtc ggcacacttt ggaatctttg ggccaacgcc
      961 tgagtttttg gggcgtggtg agcggaaccg gaaaaccgct t
//
