LOCUS       DAOQWP010000001        94417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_203_length_94417_cov_7.291219, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000001 DAOQWP010000000
VERSION     DAOQWP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 94417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 94417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94417
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_94417_cov_7.291219"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKI16_00005"
     CDS             <1..192
                     /locus_tag="PKI16_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52865.1"
                     /translation="ATPVQYSSEYNISTPGLAGGVFDDNATVDITYFGKDYVCGTVTS
                     DDGDSSIKGEFIAKYVDKL"
     gene            205..699
                     /locus_tag="PKI16_00010"
     CDS             205..699
                     /locus_tag="PKI16_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52866.1"
                     /translation="MAKPTKDAPLICTICTILALIGIVAGIVSHNPLFTIFFLLPTAI
                     YEVYRTEGESTRASSIILLLVLIAEIFLLVFNINFNLAEYLGTTEKTIGGYLVPLGDI
                     KIVGPTVMAVLAVILFLRTYGKFTKWLAVVIFVTAFAIIYTIDPVIFKDLFKYAVQQG
                     VDSL"
     gene            741..1124
                     /locus_tag="PKI16_00015"
     CDS             741..1124
                     /locus_tag="PKI16_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019234161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNR52867.1"
                     /translation="MGYIDNIEKETLENSNFRKVLFTGGHMQLVVMSLKPGEDIGEEV
                     HNTVDQFFRFESGEGKVVMNGEESIVGAEMVAIVPAGTQHNIINTSETEDLKLYTIYA
                     PANHPEGTVHATKEEAMAAEEEHHQ"
     gene            1277..2449
                     /locus_tag="PKI16_00020"
     CDS             1277..2449
                     /locus_tag="PKI16_00020"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNR52868.1"
                     /translation="MEYYSRNRNRKRRFQPHNNRGRGTNINVNRFIAKAVESEMPNIY
                     VDGHNFSEFDVVGPLRKNIQYKKYDVPTKIQHEAIPLILSGRDILGIASTGSGKTAAF
                     LIPLINKMIKDSNQRCLVVEPTRELAMQIQDEFMALARDTGIRSALIIGGSSMYAQIN
                     ILRRDPRFVVCTPGRMKDLSDRRVIDLRKINNVVLDEVDRMLDMGFIDDIKYITSRLN
                     EKRQSLFFSATMNRKSEDIANTLLKNPARVEIETQQAGKNVDQDIVRVRSREEKVALL
                     TEYLKRDEFEKVLIFTRTKREADRLSDKLFEAGIKTGALHGNKGQGQRSRIIESFKRG
                     NLAILIATDIASRGLDVNNISHVINYDVPENFDDYIHRIGRTGRAGKKGIAITFVG"
     gene            complement(2830..3711)
                     /locus_tag="PKI16_00025"
     CDS             complement(2830..3711)
                     /locus_tag="PKI16_00025"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNR52869.1"
                     /translation="MINRTVLAIGINIPAQEKVAIQKKLKLKDITLDIASIKDIIFTI
                     IDGEIEVEIKDFDLKKYDYIWIQSGWNTTHMAYLLHLYLKSKKIPHNKTNAHNTKLSD
                     IFSLASKNILVPNTFFHNGLKVNNENISDIEKICKLPCIYKTSLGSLGSHVYLIDKKT
                     DIEQTIKENGKFNRYIFQEFIPNTFDYRVVISNGKSESVCKRTRKEDKYRNNVALGAS
                     EDFINVKNVSKDILELAIQSAKALKLNWAGVDVVTDTNTGKNYVLEVNRRPGLTVRSS
                     EISAACKYINDLVNIKL"
     gene            4027..4785
                     /locus_tag="PKI16_00030"
     CDS             4027..4785
                     /locus_tag="PKI16_00030"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNR52870.1"
                     /translation="MIYTFIAIFVLQIIFFVYAALKKTDKVTDLSYGLTFIIASIFAY
                     VLNIEYASFFKIILVLLVSIWGIRLSVYLFVRILKTGKDKRFDGVREDLKRFASFWLL
                     QAISIFVILLPTTYILTSDMDMSLNWISLIGLILAISGILIEGIADSQKFVFKKKVEN
                     KGKWIDTGLWKYSRHPNYLGEIMMWVGVFIYCFVYINGIGLIAILSPIYISVLLLWVS
                     GIPTLEREYDKRYVNNKEYQAYKKDTGVLFPKLF"
     gene            4935..5582
                     /locus_tag="PKI16_00035"
     CDS             4935..5582
                     /locus_tag="PKI16_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52871.1"
                     /translation="MVNKKSSIPPKNIPLSEIGGMIKIAFKISSTGVEITYDKNLDIQ
                     KMFFILLYLYTSILIRAINNLGSASAQSSFISLDYNPRNKRISYKQGSIGEKHFTGTF
                     EISDNAKNLLQLNLEGFKFFSSDASTELIMSLKKLQEFIIYTYKDEKKIHYAFRVIYK
                     LLIRDNDNFEVTSKASSMEQMQIFDEIALQIHYFLEDTEKWFDTYGEEILQDEPL"
     gene            5706..6332
                     /locus_tag="PKI16_00040"
     CDS             5706..6332
                     /locus_tag="PKI16_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52872.1"
                     /translation="MEISEYIKKKYGNREHSLIVTLKRYFSEGSIDVVKPLKKKWQNI
                     LKTEDFLEKGFDELYSSLIETNTREKPGGLDEFIIKKVYLAITIGFLLAIINNADKKK
                     FRELATSGTLLLYLQDIKLPIFDEYMQEYIVPFFDEKAEKGLHSIILLLVDELIAKDW
                     LESLRNCYSSVNLSIEKDQLSEELIKYYFIKKDELSVETDNYIEKLYK"
     gene            complement(6400..6672)
                     /locus_tag="PKI16_00045"
     CDS             complement(6400..6672)
                     /locus_tag="PKI16_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52873.1"
                     /translation="MNIIALLILSVFITITISWVVMMFVMKSTTGELKHFITPNNLVK
                     ILAIIVVSLGVIVLASEKLVSGESVITILAGIIGYTLGTSFEKKDN"
     gene            6790..7623
                     /locus_tag="PKI16_00050"
     CDS             6790..7623
                     /locus_tag="PKI16_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52874.1"
                     /translation="MPKLTQVYKCEDKYDQILINKCLFLVMDNNTEQAIKIYQKEIGN
                     KNINANKYVENIFNTIDSIVKAKHQWKMYQKLEKKVIKKIKKEIGKSKTVCDQGFAEP
                     EAISHFDAYLTQLKTALDSLALAIGSLYGFHPDGWHKGKNEKGERVSGLRLINALENL
                     GIKQQRSVELILNYIKEQIPTINKLVFLRDGSVHKKGDRKREITDFVYYQNNEVIQSP
                     LLITDDGNLFLSKVMERSIDYFCNFTLTVIILSLSNLTPLMKPVIDEKNEKVFWFFNF
                     K"
     gene            7726..9477
                     /locus_tag="PKI16_00055"
     CDS             7726..9477
                     /locus_tag="PKI16_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HNR52875.1"
                     /translation="MSKTIFKTIQYPIQTLIDDIDHGKIALPDIQRPFVWNTTKVRDL
                     FDSIYRGYPVGYLLFWENITDKNRKDIGIDTKESNAKNLIIDGQQRLTALFSTIKGIP
                     IIDENYKEKRIKIAFNPLKEKFEVSNVATDKDVEYISDISILFKSDFSEYRFINEYIG
                     KLKENQEITPTIEEMVSENITHLRSILGYDFTILEIAENVDEETVANIFVRVNSKGVV
                     LKQSDFVLTLLSVFWEEGRKKIEEFSMQSTNSNLKDLKFSSFNHLIDVKAGEILRAIV
                     GYGFHRGRMSDVYSLLRGRDFETREYNEELKKKRFVDLEGFVSNGLDNTTWHDYINII
                     QSLGFKHKDLITSRTNFFYSYAFYLIGKYKYNIEFHKLDRIVSRWFLMSALKSRYSGS
                     SESVFESDLALVRNIETGDEFISLLNERIDSEITNDFWEITMPTLLTSSYAKNPQWLI
                     FVASQIRNNVELLFSTKKISDLFDPIITPKKARLDRHHIFPKNYLKNIDVVLQTEQNQ
                     IANYVYLDYMTNIKISDDSPTNYFKGFKKYSKEDINIALKNHALPTNFYKMQYSDFLV
                     ERRKLMAEYIRKYFESI"
     gene            9480..12563
                     /locus_tag="PKI16_00060"
     CDS             9480..12563
                     /locus_tag="PKI16_00060"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR52876.1"
                     /translation="MDITQKDTKEICNQYLKEVSLKYSDINTSEYGYRTPFENFLKEI
                     FSEIKVTNIDHDGKSVGGNKPDFVLSEGSAPLLYIEVKDIGVSLDKIEKSEQLARYFG
                     YDNLVLTDYLEFRFYRNGLKYVEPISIASYDKNERTISYNQENYELLRKTLIQFTQSH
                     KEPIKSGSHLAKIMGGKAYRIRENAREMLNSPGKEKRSIYKVYETMKIQLLHDMSSDD
                     FADMYAQTLVYGLFVARFHDKTPDTFTRSEARDLVPASNPLLRHFFDHIAGADFDDRL
                     DSIVDELCRVFSHSDISKLISNYYGTEKGSKDPVIHFYEDFLQEYDSKKRKEFGAYYT
                     PLPVVKFIVKAVDYILEKDFKLVGGLANSSKNEKGHIVQVLDPATGTGTFLTEVIKII
                     HERREKQAGNWGSYVLHEVLPRLFGFEIMIAPYTIAHLKMSMLLKETGFKYFNNRRLG
                     IYLTNSLQEGNTIENMFGGFGLADSISQESKEASVIKNEKPIMVVLGNPPYSGVSSNE
                     TPYANSLVNKYKKEPKGGKLQERKHWLNDDYVKFIAFAENLIEKNETGVLAFINNHSF
                     IDNPTFRGMRYHLLKTFDDIYILDLHGNSKKKETSPDGSKDENVFDIQQGVSINLFVK
                     TGKKKPEELGSIWHSDIYGDRITKFNILEKSQLKDINFEKVSNREPFYFFKPINQEGR
                     EEYDRGFSVKDLFNKYTTGIVTAIDRLSIFQSEKELREKTNEILNSSNPFQEFDIKDQ
                     RKHTKEERIEELRKASENLPTKILYRPFDIRYMFYTKTSECWINSPRYEIMQHLIGGD
                     NLALIIPKQTKGKIGASITRYISGHKTFSSYDINTLFPLYLYDVEGKKTLNLNKSILN
                     EFEDSIGQLEPEKLLDYVYAILHHPSFREKYRKFIRVDYPKIPYPKDRKTFDKLAKLG
                     EQLVKYHLLEHENIDPYQISYIGEGENIVEKPEYKDGKIYINPTQYFGNVSEIAWDFY
                     IGGYQPAQKWLIDRKGKELTPDDIEHYSKIVLVLKETDRLMKEIDKVNWSKDNEK"
     gene            12553..13671
                     /locus_tag="PKI16_00065"
     CDS             12553..13671
                     /locus_tag="PKI16_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52877.1"
                     /translation="MKNNVKTKKRTTPQFSKVVNTSLTFDLTSYKRVVTQIDSILESQ
                     KNLFSSIDNKLDVYKNILDSELFKPSNSLCKVLEEATKPSKALQMLVESMNYPSNALV
                     KTIQEMTKPQKLLVNSIEESLKSITEPFSKIDFTSYLSSISEIELSLATNLVVKTELL
                     KPFEGISNGVSTNTAIIDKRLINNGEIGLRVQKDTATISTQIQYQKFEMIDTKLSIIQ
                     TKVDNFEQDITKELKERFVTLDRIIEEVENDPFKFFKVKSFEFIKEGSTFVINNVRKI
                     SLVSGTIQDYICQILFSGIKELGEEWYWEDIFDEMVAKYRYEGKCEMTWKSMQDAIGQ
                     LNIKIATETTIKDAFLTPRSEIIQLNPLYFSSDLVRKQ"
     gene            13957..14151
                     /locus_tag="PKI16_00070"
     CDS             13957..14151
                     /locus_tag="PKI16_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52878.1"
                     /translation="MVSEEGLIKFKKIYEENYEIKLSRKDLIARANKLLNLYKAVYGQ
                     RTNIKIDIKNEKEIQLEKTE"
     gene            14117..14557
                     /locus_tag="PKI16_00075"
     CDS             14117..14557
                     /locus_tag="PKI16_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52879.1"
                     /translation="MKKKYSLRKLNKHKSYDSSELQEILGVHPQTVKTWKKEGLNPIN
                     GGSSPFLYSGEELITFLLKRTESQKVKLKEDEIYCLKCRRAVKPIDTEVVRRGVILGS
                     GNESTILKSNCPICKSKIQRFSSIKREVDCEVETNEKNQLDLFR"
     gene            14641..15690
                     /locus_tag="PKI16_00080"
     CDS             14641..15690
                     /locus_tag="PKI16_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNR52880.1"
                     /translation="MGNKLYQNEKMKRKYFDFCRNAKGFSIKTMKKLEGAVYKYDEYS
                     KNIDYKQFNSEIAKGFKKWLASHKNNKKQLLALSTQFHILRHLKGFLTWLSQQSGYKS
                     KISFDDIEYLSLSRAESRMATASKQPSYPSREYILKITSFKINNDIDLRDRALISFTA
                     LSGMRDLAIITLPFGAYDKENQIVNQDPSLGVKTKFSKTISTKLIIFDAKLLEYFHLW
                     VERLKNKYIFSINDPLFPATEVGLKSDVEQSFIAIGISKSSWNDAGAMREIFKKRAMQ
                     MDLDYFSPHKFRHFNTSEALRYATNAEQIKAISQNLGHERVSTTLFSYGAIDNFRLPK
                     VIEEINEGVKNGNNL"
     gene            complement(17337..17413)
                     /locus_tag="PKI16_00085"
     tRNA            complement(17337..17413)
                     /locus_tag="PKI16_00085"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17377..17379),aa:His,seq:gtg)
     gene            complement(17439..17720)
                     /locus_tag="PKI16_00090"
     CDS             complement(17439..17720)
                     /locus_tag="PKI16_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52881.1"
                     /translation="MPEYVYSIILVVVIVVIMVWWVSKKKGEIWKGTFEKKNYVSGDE
                     DSSDSYYIVFKTDEGKKKRFTTRDMGYYNSWNVGDRCEKRKGDFFPVKV"
     gene            complement(17742..18290)
                     /locus_tag="PKI16_00095"
     CDS             complement(17742..18290)
                     /locus_tag="PKI16_00095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNR52882.1"
                     /translation="MRNTTILFILFGVVIVGALIFTFYFLPKLDASSNILNLKMALPV
                     ERLDMRYIEKGITPFCEKKSHGIVIETKERAQVMAPVDGTIDSIEEDVVYLRSRDNVL
                     IYFSSLTNIRVSVGDYMTSGDILGYVDGNMFEFGIDNSMDSRYECPFLYLDDKGQEII
                     SNGLKEGIEVKSRACECSFVSY"
     gene            complement(18308..18985)
                     /locus_tag="PKI16_00100"
     CDS             complement(18308..18985)
                     /locus_tag="PKI16_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52883.1"
                     /translation="MEDTTVKNPPIEDSKPKKKANGCLIAFLIVFLLFGLLIGGAYFG
                     YKKIMKGLNTPSDLGVTYSMADLEESFANAGFTGDMCLDCTTPIYSKPHEVEVTFTSS
                     QATAWLNYANHDLPYGELDDLQIKFSDGKAEISALFEYEGKVYPVYVSGTGEKASSNS
                     ITGQLDSLKVAGVSLPVYLVPVVQSALLELANEKLANMGDTLRIDTLEITETGLNFEG
                     LAPSVMK"
     gene            complement(19050..19733)
                     /locus_tag="PKI16_00105"
     CDS             complement(19050..19733)
                     /locus_tag="PKI16_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52884.1"
                     /translation="MEDTLQSELEITQPVETAVEIKPKKKVSGCLVTFLVFIGMFLLL
                     IVGAYIGYRKISSDLKTQVDLNVRYTDEDVIDLTEIVKITPENYMDIKDGVDISFSSA
                     QITAMLNAQSVSPTFSNTQIKFNKDNVQISTLAKFEFSEGEPFNLPLYVSANSEEFTR
                     ENFELDIQEIKIGLLRLPDWVQEKIAKTEEEWIAGVILEGLSSFEFESIRFTKDHVDI
                     QDLLFGALK"
     gene            complement(19771..21540)
                     /gene="glmS"
                     /locus_tag="PKI16_00110"
     CDS             complement(19771..21540)
                     /gene="glmS"
                     /locus_tag="PKI16_00110"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNR52885.1"
                     /translation="MCGVFGYTGKQIANDLVFQGLKRLEYRGYDSWGIAVNDKQHIQV
                     FKTVGEITERQELKKLPSVNTAIGHTRWATHGGVTTNNAHPHNSENKFFTLVQNGIVE
                     NYSELKKSLEQKGYVFKTQTDTEVIVKLIEDEQKTKKPFKTCITKAFKLLKGRNTIVI
                     LTKDNDIYAIRNGSPLVIGKSKDGIYLASDALSFSPHTNKVVLLDDHDFVHISNGKIE
                     ITNILSKKKNVKIHVLKNKYVDINKGKYAHYMLKEIVEQQNTIISATQYTKKELKPLV
                     EDIQKAHRVYVVGAGTAGFAAGQIAYFLRNISGIDAQDVKSYEFKSYINILSSKDLVI
                     AVSQSGETADTIEVLEIAKKKRCKIASIVNMLGTTISRMSNHQYFTNAGPEICVASTK
                     VFTAQCSWGYLLAKTIANEYDQAQKDVANLSVKLGKFLHIDTYKNVKKIVKKIKNSEH
                     LFVLGKGSNANISLEGALKIKEISYKHIEGFMAGELKHGVIALVEKGTPVFGIISDDQ
                     DSKDMISSMEQVKARGALTIGVGDKKFSNTLFDFFIPTPQVNGLSGLSNVIAFQLVSY
                     FLSLELGNNIDKPRNLAKSVTVK"
     gene            21769..22476
                     /locus_tag="PKI16_00115"
     CDS             21769..22476
                     /locus_tag="PKI16_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNR52886.1"
                     /translation="MLKEKILTELKSVSKKEKEEIFQRFFKTGKGEYGEGDIFAGITV
                     PEIRSISKRYYKEMTLEEVEYFVQHKIHEYRLFGLLTLTYMWKKADEVKKKTVFNLYI
                     KNLKYINNWDLVDLSAPSIVGEYLKEKDRTILYDLAKSNDLWKQRVAILSTFSFLREK
                     DFVDTLHISEILLHHKHDLIHKAVGWMLREIGKRDQDVEEEFLKKYYKEMPRTMLGYA
                     IERFEEEKRQKYLKGLV"
     gene            complement(22460..22972)
                     /locus_tag="PKI16_00120"
     CDS             complement(22460..22972)
                     /locus_tag="PKI16_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52887.1"
                     /translation="MILLNKQLEILKSEKIQLLITYSLAILLPLLIPKPQILLGTLIN
                     ALFIFSVTRFGLRKTLPLLLIPSLLSYTQGLLFGSSTIFLLYLIPFIALSNFALVYLY
                     SKIKLPYVNILISASIKALILYSAVLLLMRLVNMPGAFLQAMGMIQFITAVLGTTFVT
                     LLLRKFKPNL"
     gene            23061..23912
                     /locus_tag="PKI16_00125"
     CDS             23061..23912
                     /locus_tag="PKI16_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNR52888.1"
                     /translation="MEFQSGSKYLCLLAPSFVSEFEYPEVIYRLRMLGFDKVLELTFG
                     AKMVNHAYYEILKESLEKGEKKTWISTPCPTLVNFIRVKYPELVDNLVPVLSPMGAMA
                     LICDKFYPGYKKVFIGPCVTKKLEAGEIGTVDEVLTFKELELLFKEKNIPEKVTDSKY
                     CVTFDKFYNDYTKIYPISGGLSSTLNYSDILKKKDILVMEGLNDIMKILGGFKDGMYK
                     NYKFLDLLNCKGGCINGPGMIGDRSVKERRKRVIQYRDYAQRYEKDLGKAGIKVDAEG
                     IEFGRKF"
     gene            complement(23921..26029)
                     /locus_tag="PKI16_00130"
     CDS             complement(23921..26029)
                     /locus_tag="PKI16_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNR52889.1"
                     /translation="MGNSTLDPKSVAIVGASTDSEKIASVILRNVIEGGYNGKIYPIN
                     PKYEEIQGRKAYPTILEVPDSIDLVCVAIPYQFVDEIVDQCIEKKVKTIVIISAGFKE
                     TGEEGKKLEESITQKLKNAGIRLLGPNCLGFINNRAKLNLSFARENPGEGNIAFISQS
                     GAFCTAILDMACEKGLGFSHVISLGNKADIHENELMAQLFADSNVKALALYLEEFKDG
                     KEFVSLSQKAKKPILLIAPGSSEEAKLAISSHTGALASSYDTTIAAVRKGNIILAENS
                     EELFKLISLININAIPKGNKIAIITNAGGPAIIATDMVEKEGLELTEIGEKTKQNLLK
                     ALPRESSIKNPIDILGDAKSDRYELAIKTVLEEKDADSILVILTPQLITEIEGTAQKI
                     VEISKTESKPIYSCFLGGKDIKAGFRILELNNAPYFNDIQEAVHLIKKLSVFEEEKDN
                     KKIVEANSLKKRGKYTKQIKKLLKENTAVIVPDELAISIMREFKIDIPNELVTGSIEE
                     AINFAAINFPVAIKATAEEFEHKTDFKAIYLDIRTITEFEEKFEELKTTIEKVTGKQS
                     PSILVQEMIDGKLEFFIGTNREGNSNIYEKDGLGFGHLLAIGQGGIYTEVYKDIKHIL
                     LPESLENMDSILNETKVSTIIDGYRGKPKLAREKLLDLMMKLQKMLISYPQIVSMDIN
                     PVMLTEKRAVVVDIKLYIKE"
     gene            complement(26033..26860)
                     /locus_tag="PKI16_00135"
     CDS             complement(26033..26860)
                     /locus_tag="PKI16_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNR52890.1"
                     /translation="MKNSFDSEKLPTWCPGCGNFTIHASLKMALESLNIKPSQTFISF
                     DIGCNGNGADVINTYAFKGLHGRSIPLAVGAHLANRKYTVIADIGDGGCLHEGLDHLL
                     HAIRSNYDITILIHNNQNFALTTGQATATTQKDKSVFGFPNGKPENSINIGQLCLSLE
                     ASFFARGYSADPQQLTEIIKEGVKHKGCSVIEILQICPTYNPQSSPQWYNERVKKVET
                     VSDIQKARELVEITDQNIATGVIYKNSKLPTYYDSLPNRKERKTELVDEVREYNIKI"
     gene            complement(26850..28502)
                     /locus_tag="PKI16_00140"
     CDS             complement(26850..28502)
                     /locus_tag="PKI16_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNR52891.1"
                     /translation="MSLTRYSVKVAGQSGQGINTVGAIISKILNSVGYITFSYREYPS
                     VIKGGVASYQIDFSDKQINCSSRYCNILCSLDKNALEEYLPTLQKNGIVIHNLKDYTK
                     EIFIDSRQIISELKAPEIMENTIMIAFICKILNIKKNIVEKHILEYFKEKKVDSDLEK
                     KCIEKGYSIEYVKKIELPTVPFRITSKKILNGNEALALGAISCGVRAYYAYPMTPITA
                     IFKHMQEYGTEKGVLLKQAENEITAILMALGSMHMGTRALVATSGGGFDLMTETISCS
                     GMTETPLVVILGQRAGASTGVPTWSGSSDLNIAVKAGHGEYPKAVLAVSDIESSYTLI
                     QKAFNLAEKYQIPVILLTEKQITESLFTVNKLPNNLPVRRYLSNMTFRYNITDSGISP
                     RRIPTFDEQPYLSSSDEHNEKGESIENSKEIKQMTEKRMRKVKSLEKEFPQPLLFNPH
                     KSKTILVGWGSVKNTVLDLISQGEDIGYLHYEYLFPLKTELLNTLIKRGKKIIVIENN
                     QTGQLAEMLKEKTGYSFKKRLLKYDARPFFVEDILEYLKNEK"
     gene            complement(28517..29440)
                     /locus_tag="PKI16_00145"
     CDS             complement(28517..29440)
                     /locus_tag="PKI16_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52892.1"
                     /translation="MKNIDNRYNAQALAIAMVVLVVSSIIGLAIYSRAAKDKLLSVGE
                     RASAEALEVSDLILNQLTTVPINTITQTIAGEGNTFDYENGVTLTESNGQISDLFTAL
                     GIEEIFSTLNFCPIRTGGNEYYLKIKEASPDTYFEIRAGQVMSFPIKGVPVGAGCRAS
                     VSFATRGDSQVGFSVEKIYGKNYTNGIAQKYKPYAIDDVKQYCFSCNETKFLGDEWIP
                     IADGGTLGFNLNELSGTYALDEIRVKAIGGTLGVGYALTTECTQGFRIVDVQASANCD
                     GTYRGKQILVPERKWSSPVFDYSLFNGQGSL"
     gene            complement(29443..32070)
                     /locus_tag="PKI16_00150"
     CDS             complement(29443..32070)
                     /locus_tag="PKI16_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52893.1"
                     /translation="MIKRYLSILFLVAIIGLLQINKVSAELVCGGTWCSIGTISCPCP
                     DGTCYMHCTRACRTCTETSIPETKTSCSSIDGTYPNGSSVECCVRDCDSTCTLPACPR
                     GYTDNTSLTSCTTVYTPKPSCDIGGEDCGTDCGSRTRNCYLLETNVAPSMPTSVKIVV
                     DNVTYNLSTNSNTPTRIKLPSTASNVQVKEPTTTFPSTSNGGGYAFKGFNDGVNDEWA
                     AITTCTPTSCAGVAGEDFFIKDTVNIQDFDPTTKTILQVLKENADGKIAGMYYTINKC
                     NTTKQYSLPIEGYYEVDNIPDSNITIDITIDKTAKNCTSTTYTGLDINNPLKFNASVT
                     DADSNDEIEALIIWFSKDETVPPLVNITGTYTQSNTNDFGIMIRKNAGSWNTPLIYST
                     NTDNTWRLLTAADKLAVQGLTITQGASLDISFGLEFKPTANNPSGLYNVYGTAVDSYM
                     INNNIVDQSRIQDLFNWGIDLANPTVNDITQTVKDVNSVYLDWSANDNESSIMRTVIN
                     GYRTGGSITNDLEMFLPPDYTTSKGTITLTQIPEETLIGMFDDTNAWRFTNTSSQVDL
                     INVGENEGGMISTYVTAYDMGCNTNAQYKDINLNPWITSKGGFVYSSSGITNAAKDVA
                     GLPALDGVFVKLTSAELDTGTELISARNDILPELLHPELKAVRALNVYDSNDRKTYWF
                     DHFKEKLATEKVPGTTKIATPTSSCASGICYVYSTNNITLNTGFNCTSKILVMSEGNI
                     TINPDITSSSTNTGCIFLAKGNIYIGAGTYKTGVNTNVHYDYLDAYLMAEGQIIFNLV
                     DTDKSVRDGIEINGGMVAFGNGVTGGSAINVLRNLKLSNISNPTVVINYDYKYPNIAT
                     QFFGVEAPIYKQEIGFKSF"
     gene            complement(32067..33236)
                     /locus_tag="PKI16_00155"
     CDS             complement(32067..33236)
                     /locus_tag="PKI16_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52894.1"
                     /translation="MLKLSKKFALPLIAVLIIGTGVGAYFLFRNFEIPEYFVDRYPEL
                     LEYCDVEESEDSLKVSCKALILEILPNAESPSLVSLIITKDEELKEYTITEKEEVFGF
                     TNDILQFKKLKPIVINIQYTKINPIKYGVSSITFEDIPETYIQEIVNRDIEEIIGMDK
                     NSTTILNSFDFCPRPETLPDYVIKKDEYAKYWEENILSEEEYKNGYNYSLDDTTIRLL
                     FACDSARNVGYTDVCNTNTIPNQSSISINNVIPFITIDFEKKQSETDTLSLLKEISFL
                     YDYEYLIEKYVQLSKQNVLENLNNAITRSNISESVFCNMYKIYSEIENESSKTYIKET
                     LLSNITKTRTGECEKLLIENNLEKEGVYLKFFLENSDPLFKIMNRCINLNLFYKK"
     gene            complement(33243..33986)
                     /locus_tag="PKI16_00160"
     CDS             complement(33243..33986)
                     /locus_tag="PKI16_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNR52895.1"
                     /translation="MEKPFLYFDTETTDANIKDLLQLAFVTDSDVQLNLFFKPTQEIS
                     FSAMAVNHITPEMVEDKKAFEQTELPKENIDPEFKGNSLIEYLNFLAEKYIWVAHNID
                     FDKEVLERKGIKLPHTICTLKLARNMFTEGDNDLESYSLQYLRYYLGLYKKENGNHNT
                     AHDALSDVHFLRDLFHYMQSNSKLTPESMLAISKEPQYMRQMSFGKYGGKTLEDIARI
                     DRSYLEWIRDSMYDKPDLQWNANRVLSVT"
     gene            complement(34002..35447)
                     /locus_tag="PKI16_00165"
     CDS             complement(34002..35447)
                     /locus_tag="PKI16_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HNR52896.1"
                     /translation="MLHSIVNIAKILKGVLLVLFLSLFLLPSNIQAQEIIQLKSVTYF
                     TGIGCPHCAKVTPVLHEKVNTDGNFLLIEYEIYQQSANAKYLNTYGSRYGSGLGIPLV
                     LYDEPNYDLGDVNILKNFDEKLANIPVNTFELTNGYMDTLTAELNKFEGKPKIYGKDR
                     VAYQDTNKDLSPEENSIVRDFLLAQDLNAYVKELEGKKTNNLTIKISGAEEVYDYGIS
                     VGSWNILWRGDAPQGTSTGSNTSVDEPQTATDSNISILKVIGLALTDSINPCALSVLL
                     MMLIAVTTYHPKDRKQILWSGLAFIAAVFITYFIYGLLIVKAFEVLQSFSNIRLYLYK
                     GLAIGAIVLGLLDLKDFFFYKPGGVGTEMPLFLRPKVQKILAKVTSPLGAFGLGMFVT
                     LFLLPCTIGPYIILGGMLSFGEFFQSVPILSLYNLIFVLPMIVIVFLIFFGSKDIKQI
                     SSWKEKNIRFIHLVIGLVFVLLGTAMLLGWI"
     gene            35529..36644
                     /locus_tag="PKI16_00170"
     CDS             35529..36644
                     /locus_tag="PKI16_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR52897.1"
                     /translation="MQEWYRNPKLQYLTFGGYAGTGKTTLLGYLGLKIHDENKKIRIA
                     YCSFTGKASRVLDRKLKDVKALTKGDYTGTIHRLIYKAIVDDNDNIIGWEKKPKEEFE
                     YDLIVVDEASMVTKDIWNDLLSFGVPVLAVGDHGQLPPIESSFNLMERPVLKLEEIFR
                     QEKDNPIIQVSEMARKYGGIPKMAFSDTVRKLSKQDEDTQEFVTDIFSSFNDQTMVLV
                     GYNNTRINLNKGIRQLLEFENQAPTYGDRVICLKNNANAGIFNGMMGTVVSMSKSDIK
                     GCLDAEIALDGEDECFVGLISEKQFNNSLVDGKTLMREINLFDFGYALTVHKAQGSQA
                     RRVVVFEERFARMTDDLWRRWLYTAVTRAEKELYIIG"
     gene            36661..37425
                     /gene="map"
                     /locus_tag="PKI16_00175"
     CDS             36661..37425
                     /gene="map"
                     /locus_tag="PKI16_00175"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNR52898.1"
                     /translation="MIIKKPKEEKILREAGVISVEILSKLKDTIKEGVTTLEIDEHAG
                     ELCKEYGVKPAFKEVDNYYFNTCISINDIAVHGLPSKYVLKKGDLVSIDFGIVYKGYM
                     TDHCWTWCVGKPDERKKKLLESGRRAVENAVEKAIAGNHTGDLGFEMESEAKKNGFNI
                     LKVFVGHGIGKGLHEYPDIPAFGKKGTGDLLVDGMVICVECQVVDDTGKISVDDDGWS
                     ERTERGGDSVMFEYMVIVKKNSPVILTDTRDWGVVV"
     gene            37583..38107
                     /locus_tag="PKI16_00180"
     CDS             37583..38107
                     /locus_tag="PKI16_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52899.1"
                     /translation="MKKLIVILVVLFLAIPSGKASADFVCDSACVERILSGGISSEEI
                     SYYGTGNLLRVLTSYYGGDTWMALRMLNLASNTYTAVNTVNYVYGTQFKADTIGFYQC
                     QSKYLYCEEGQFLGYFQEDDKIYRVICTPGEICQEKIILEDEPLNLLNEVFFQSCYYG
                     GPGEWGISCEEVPR"
     gene            39357..39707
                     /locus_tag="PKI16_00185"
     CDS             39357..39707
                     /locus_tag="PKI16_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52900.1"
                     /translation="MEKKWSLIIILIVIVSLFLYFTGFASADSWCQPDDVVCLEKETN
                     PKYIASFQVMDSNGRIVDSGQWKNSFDLFFNNPGCIYLQPRTQTTFYSDWDRNWFWEK
                     KTDLFGNVCYKLKK"
     gene            39844..40785
                     /locus_tag="PKI16_00190"
     CDS             39844..40785
                     /locus_tag="PKI16_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR52901.1"
                     /translation="MPENNIYDIAIVGSGPAGYSASIYASRYKLTNIVFGKLIGGTIT
                     EAHKVCNYPGLPDISGIDLGYKFHEQAKEQGAQHSTESIVDIKKEGNIFKLFTDLNKE
                     YLAKTVLLATGTERNKLVIPKEEEYVGKGLSYCATCDAMFYKDKVVAVVGGSNAATMA
                     ATMLSDIAKQVYIIYRGTELRGEPAWVEQVSQKKNIQTIFQTLVVGLEGEPRLQRVKL
                     SKPFNDSEYLEVDGVFVEIGAEPNITLPVKLGLELDIQNYIKVDEGQATSIEGIWAAG
                     DCTTNSNKFKQLVTAVSEGGIAANSIYCYLKQASCSH"
     gene            complement(40769..41260)
                     /locus_tag="PKI16_00195"
     CDS             complement(40769..41260)
                     /locus_tag="PKI16_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNR52902.1"
                     /translation="MILDIGEYPKFRDENLKGKIVVVTSGFFDPLHPGHVSLFEESKK
                     LGEVLVVLLNGDMQAVAKKGKAFMPAKDRAYIINAFKCVDHVVIYDAEGRTDSNEAIA
                     IMKPEIFAKGGDRDMKNNVPEVDTVESYGGKVIYNVGNPKIWSSSNYLEEWVEFKNAS
                     ENN"
     gene            41458..41907
                     /gene="nrdR"
                     /locus_tag="PKI16_00200"
     CDS             41458..41907
                     /gene="nrdR"
                     /locus_tag="PKI16_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNR52903.1"
                     /translation="MRCPFCSCDTTKVVDKRDNREDSSTRRRRQCLRCGKRFTTYERI
                     EKVDISIVKKDGSLEKFDVEKIIKGIKRAVNQHSISDSEIYNFAEEVEKYVFNSEDSV
                     TSRDIGLMILNWLREKDPLAYIRFASVYKDFKDLNDIKDEIANLEKS"
     gene            41925..46151
                     /gene="nrdD"
                     /locus_tag="PKI16_00205"
     CDS             41925..46151
                     /gene="nrdD"
                     /locus_tag="PKI16_00205"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF026240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNR52904.1"
                     /translation="MAAQKKKITLKVRKRDGRIVKFERERIETGIFKAAENVGGKDRQ
                     RAVEITDEVVKRIQEKYKGKKVIKTEAIAEIIKQTLIDQGHGKTSISFGLFVDLKNQI
                     RNIKSLIDANTLVKEYIDNEDWQVNENSNMTYSWQGLNNFISSTVQANYWLHSIYPKD
                     IANANIDKVIHIHDLGMLAVYCCGWSLEDLLLKGFTGVPGKISSSPAKHFAVALGQCV
                     NFFYTLQHEAAGAQAFSSFDTYLAPFVRYDKLSYKEVKQKMQEFIYNMNVPTRVGCQC
                     VSEDTQILTPDGWKGYKDISKGTVIKTYNLETGKIENQEATSVFKSEYKGIMYNLKNR
                     IQDQLISPNHRVVRKLFNSDKYTLEPIEKVMELKSPFIVPVSGENSNKAGRITDEQIR
                     LMAWVISEGSIEKEGSKRISIYQSKIKNPKKYKEIIDLLSYFKFTFTEVERTGFGKPV
                     KRLRLDAKGSKTIMKWFGAESNIKFLPKSLFNLNAAQSRLFLDTYLKGDGFEECKITT
                     SEQEILDGLQVLAVNAGYGFTVLERKPTIGKKNLFVLRLIKHKETYIKEAKKVNYEGV
                     IWCPHTVNETIIAKRNGKVFITGNTPFTNITMDLIPSGTLAEQNVIIGGKPQKEKYKD
                     FRKEMGMINKAFCEIMMEGDSQGRLFSYPIPTYNLTTDFDWDSPDYTPIWDMTAKYGI
                     PYFSNFINSDMKPDDARSMCLDSKEEVLIRDAGNIKKRSIKDIVERYMDGNFDKDGWA
                     QCKKSERLEALSLNPETGKTEWTKVTKFLKVSDDLSVKIIMQDGKEMVTSSGHLVPVL
                     TKDGLINKLAKDVKKNDYLLSLKSSSELSSKYENLGNNLILDDNLAKILGYFVADGNF
                     LKESRKNMQRYGQPRGLQFTFNAKTKEHLHEIKDLLNNHLKLDTKEKKDPRYNSYYLY
                     VYNVDIAQTIYNAGFKKYGKLPDILFNSPKSVIENFLTFHFKGDGYEKRSEIHINDKD
                     LAKDLVMLYSLINKPVIYKERKNSQTIYIQHQRNKLNSNGFLSAPILAERVPGFLAKS
                     TYLVPGLNKSRMVGFNSLEKYSAQTLESKLIEKGDYYIVRVKDISLNKLRDSQDFFDL
                     ELEKNHLFVHSLGTITHNCCRLRIDNRELKKKGGGFFGANPLTGSIGVVTINLPRIGI
                     ETKTKEEYFNHLDRAMDLAKRSLVIKRTFIEKYMEKGLYPYSKFYLSDIKKRFGEYFK
                     NHFNTIGILGMNESLINFFGDMEKGITSKEGREFALEIMDYMREKLKDYQKETNQLFN
                     LEATPGEGATYKFAKADKKKFGDLAITAADIAEKKGMIPYYTNSSQLPVGLTDDIFEA
                     LDLQDEFQCKYTGGTVMHLYLGERVSSADATKNLVKKVAENYRLPYFSITPTFSVCPK
                     HGYITGEHKYCPKCDIEVGYKDGMKFSEKF"
     gene            46175..46306
                     /gene="nrdD"
                     /locus_tag="PKI16_00210"
     CDS             46175..46306
                     /gene="nrdD"
                     /locus_tag="PKI16_00210"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNR52905.1"
                     /translation="MKKVTRQRCEVYSRVVGYIRPITQWNIGKQAEWSDRKFFKVTE"
     gene            46318..47013
                     /locus_tag="PKI16_00215"
     CDS             46318..47013
                     /locus_tag="PKI16_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNR52906.1"
                     /translation="MVQIGGLEKTSLLDYPGKISAVIFTYGCNLRCSYCHNPELVIEE
                     FDKEHSISEEELLRFLEGKKGKLDAVVITGGEPLLHPDLEELIVKIKEMGFLIKLDTN
                     GLLPSKLENLIKKGLLDYIAMDVKYPAEDYPIMTGIPDVIHKIKKSIKIIMNSGVDYE
                     FRTTYVKGIHTFRSAKYIGQLIHGAKLYYIQNFRDGKTITKGFDSSNSFTDKELKRIK
                     REAGLYVKKTIIR"
     gene            complement(47006..47656)
                     /gene="lexA"
                     /locus_tag="PKI16_00220"
     CDS             complement(47006..47656)
                     /gene="lexA"
                     /locus_tag="PKI16_00220"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNR52907.1"
                     /translation="MDSILTAKQDLVLRVIRDYFLKNGYAPSLSELQVLLDIATKRGV
                     VNHLIALEKKGYILRTSEPRGIRMLEENEEPVYEYLIGVPILGYANAGTPLVGAEEEN
                     LGVLRVDKNILGKKKSLFSLIVKGDSMDREMIGGKAITDGNYLVVDKEAEIFDGDVVV
                     AIVENCATVKRFKKDQDMVVLYPNSSNPIHQPIYLDKNTESMLNGKVIKVLEKPII"
     gene            47799..48134
                     /locus_tag="PKI16_00225"
     CDS             47799..48134
                     /locus_tag="PKI16_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52908.1"
                     /translation="MAEEKKAKKIYTLEEIKFNEANKAMSILACIPIVGLILLFVEKE
                     DKFVRYMGAQFTLVGVVQLFTWVPIVGWILGPLALVLVIIGMIKASQGQRFDVPVLSG
                     LGLKLMAAI"
     gene            48221..48297
                     /locus_tag="PKI16_00230"
     tRNA            48221..48297
                     /locus_tag="PKI16_00230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48255..48257,aa:Pro,seq:tgg)
     gene            48361..48909
                     /locus_tag="PKI16_00235"
     CDS             48361..48909
                     /locus_tag="PKI16_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNR52909.1"
                     /translation="MLLYIILGVLAVLIIAVIGIYNRLAKQKVMISEASADIETFQKQ
                     RYDMIPNLVEIVKGYAKHEQETFTKVTEMRTKAMGATSFDEKAKLEDQLSNMISKIFA
                     VAENYPELKANTNFLGLQTNLKDLEDNIQKSRRYYNGAVRDFNNMIVVFPTNIIAQVL
                     GFKAEPFFEASEDEKKNVEIKF"
     gene            48918..50615
                     /locus_tag="PKI16_00240"
     CDS             48918..50615
                     /locus_tag="PKI16_00240"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNR52910.1"
                     /translation="MKKLLGYLLFVFVLFSCFATNALAQSTDVISEFKSNITLEQNTD
                     IKVEEEITYFFSYPGHGIYRDIPTKYSVQLTLARPIAIKLDELYYYKKDEPSIKYSAY
                     EKSLENGYTRFRIGEADTSIEGEYVYVIKYTMKNAVNYFDDHDELYINIAGNYWNVPI
                     QKLSTSIKVPGKIINKICYTGVSGSKESNCTFKDISETETSITLADTLDIGEDLTVAI
                     SMPKGTLKDVRGAQTVAFLLSNIGLLLPIPTLIVGLILIKKNGKDRKLTTIPHYEPPK
                     DMFPMLAGAIYNKALTPKHITAQIVQMAIDGYIKIKQEGKRNYVLEKDQIEKNIPEDT
                     VRALYADLFKGKNEVNINNIPSDFYTTVNSLKKSIDTKLYEEKYFSKESKKLWNKFLL
                     IGFFGIFVTFMAATPLASIAATGWTIGIAISSIILVILTSRIELRDTLGNKVFAELEG
                     LRMYINTAEKHRIEFHNDPEKYLGVFEKLLPYAIIFGLEKKWAKEFEDLYKEPPSWYA
                     GDISTFNTLILANSISSVSRGIQRNSTPPSSHSSFGGSGFSGGSSGGGFGGGGGGRW"
     gene            complement(50616..51677)
                     /locus_tag="PKI16_00245"
     CDS             complement(50616..51677)
                     /locus_tag="PKI16_00245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR52911.1"
                     /translation="MLDLKFLEIENDSQWDSLVLSLDNYSFLLSSFRYKYNKEINEKS
                     FRFAIYNGDMFQGVMCGSFGKTKLFGKYLEFKHSPMLKDNSEEIWRAVLEKCKNIAQE
                     NGCFMFRISPLYVDNVVLEGLYRKLGMRKSPIQDIDAMISQYVDLSKTEEELRHDMSD
                     STRNNINKLTKNTDISVKIFKDNSQFGIFADFHNQTMQKKGYTDRSTKSLLRELQLQV
                     DAGSCYMVVGYYQNKPISIWQATVYGKYMHIYQAGSDTLFRDKNIRITYLLYWECVKL
                     AKKLGCKTLDLFGGMVPEGYDGKKHPWAGVSAFKRSLGGKRVTYMHSRDYAINKAMYN
                     LYYMYSYLRTVLKGYPVSW"
     gene            complement(51688..52947)
                     /locus_tag="PKI16_00250"
     CDS             complement(51688..52947)
                     /locus_tag="PKI16_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNR52912.1"
                     /translation="MSVFISSIPNAQEDDLLLAKKITKGELDISKERMVLEDLLSKVF
                     KDSKIFLFNRGRDALYVALRAVGVSPKDEVILQAFTCIAVPAPILWLGATPRYTDIDP
                     KSFNIDLDKLQSNINDFTKAIIIQHTFGNILDLKRVRELVDEVNKERREKEKIYIIED
                     CAHHSLLSNKDVLKYSDIALFSFAQDKSISSTQGGLIVVKNQNISKKVSILYDAIPEQ
                     TEREAKYDAEYICRWGEIKKEYFKSIFTFYERLTLGKIKIILYRFLGKIKKQASADIG
                     SKKDIRRYSNEQASLLLNQLPKIKKFDEHRRIITKIYDETLKTEMKFDNHSGCLLRYP
                     VLVSNPAEVLAALAKEKIIGGRWYSSVVFPLFERFERVGYIKASCMKAELCSKYIINL
                     PTNIEVTEEIAKDIARIVNKVGISIKI"
     gene            complement(52947..53657)
                     /locus_tag="PKI16_00255"
     CDS             complement(52947..53657)
                     /locus_tag="PKI16_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNR52913.1"
                     /translation="MQKGILYLIPTPITDVPMKDILLLPYLDIVKGLEIFVVENAKTA
                     RAHLSSIELNKKIQEIEMYELSEHTKVYEIEAMIKPLIEGKNVGLMSEAGVPSIADPG
                     YRLVIFAQKNDIKVVPFVGPSSIFLALMASGLSGQNFAFNGYLPQENRDLRKAIKSIE
                     VFAKGTGQTQIFMEAPYRNQKIFENILSVCEDDTYLCVAQNIMGENEFIKTESIAKWR
                     KNKIRLDDVPCLFLINKI"
     gene            complement(53659..55419)
                     /gene="mutL"
                     /locus_tag="PKI16_00260"
     CDS             complement(53659..55419)
                     /gene="mutL"
                     /locus_tag="PKI16_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNR52914.1"
                     /translation="MVKPVLNKLSQDVVNQIAAGEVVERPASVVKELVDNAIDAKATE
                     IEIKIKDGGIELIEVSDNGIGIPKRNLPEIFLPHTTSKISRIEDLNTLLSMGFRGEAL
                     STITSVSKVHVISKFEDEGIGNQVVLNEKGQSDVKSAAKEQGTTVRVENLFYNIPARR
                     KYLKSASTEYRKIYELLNRYFIVFPNISFRIQKDGKEVLSLSALKNCKAGDICKERIE
                     EIYGHEELVEIKYEGNGMKIKGYSGHPSSHKSKNTRQFVFVNDRPVIDRGVMRAIYEG
                     YARYLPFGEKVNFYINIKINPELVDVNVHPRKEEVRFENPFRVYSAIEEAVKHALNKE
                     LSFQIDSTPSPIAQRRESFNTNSQSKDYSTREIRFDKQYSSVRDSLLFSKEALTVTEG
                     EQEDIRNIFQIFDKYIVIEFTDERLWIIDQHAAAERINFEKIQKREEKALDIQNLLVP
                     TSIIFSKEERLFLEEFQDFFKDLGINFEIKEYGIDILSLPSMLPAGNTEALFKEIFEI
                     SDNLDTLRKDFNKKKEDILATVACHISIRSGQKLSYEEMKNLFEELSSCENPYSCPHG
                     RPAIWKLTREQIDTNFERTY"
     gene            55462..57426
                     /gene="uvrB"
                     /locus_tag="PKI16_00265"
     CDS             55462..57426
                     /gene="uvrB"
                     /locus_tag="PKI16_00265"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNR52915.1"
                     /translation="MATFELESKYLASKDQEKTIFSLLEGIQEGNEKQTLLGVTGSGK
                     TFVMANIIKELGRPALILSHNKTLAAQLYEEFKEFFPNNSVKYFVSYYDYYQPEAYIP
                     SRDLYIEKESEVNKQIENLRFGAMNSALTRRDTIVVASVSCIYNIGNPKNYENKSLSF
                     FVGENIDLSEISRTLVDMRYSRDSFDFTTGAFRINGDILEIYPPYEEYSIRIELFGNT
                     VENISKIEPISKHKISDLKEILIFPATSLVYSKDVIQNAIEMIQNDLEKRTSFLKNIG
                     KTIEAYRLEQKTNYDIEMLREVGYCKSMENYSIYFDGRKTGEPPYTLLDYFPEDYLMF
                     VDESHITIPQVGGMYNGDKARKDNLIEYGFRLPSARDNRPLNFSEFEKKQKTTIYVSA
                     TPNEYEIQKSNNHVVELLTRPTGLLDPMIDVRKTEGQIDDVISEISKNVAKGQRVLVT
                     TLTKRMAEDLTSYLKDLDIKVQYLHSDIDTIERVDILRDLRLGEYDVLVGINLLREGI
                     DLPEVSLILILDADKEGFLRSKTSLIQTIGRAARHVEGRVIMYADKITESMKYAIDET
                     NRRRKYQEEYNKEFGIIPTSISKEIRGRLVEEDQKIVDNDEFVEKLDAKQKRLLIKDL
                     EQKMLLAADSLNFEKAADLRDRIKEIKANL"
     gene            complement(57416..57541)
                     /locus_tag="PKI16_00270"
     CDS             complement(57416..57541)
                     /locus_tag="PKI16_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52916.1"
                     /translation="MSRNFLFAKQTYKKAINDAAIKARRLKNLKPTIFPKSDFTS"
     gene            complement(57531..57601)
                     /locus_tag="PKI16_00275"
     tRNA            complement(57531..57601)
                     /locus_tag="PKI16_00275"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57567..57569),aa:Gln,seq:ttg)
     gene            57712..58755
                     /locus_tag="PKI16_00280"
     CDS             57712..58755
                     /locus_tag="PKI16_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52917.1"
                     /translation="MPKKHIERGIIKKRRRKFTLFKKNRSTGGKTSKTLSKGFFTVLL
                     VFVFIGIAYLSVLYINKLRSTDTNNEFDNVMIIGINDVPVFPESTFIFQNTIDQPSVS
                     KFLSEGNSAYRLPPNTSYSEVAQFYNKKLPEYGWEFVLSVSVGSEDMRYGEYWVKEDR
                     GLRIYSKFNDIWYESITVEEARTGLAQEVKDEIARNLLLADSDAQDLLPDFPWIIEIP
                     KEYVISYFVSPYKNESGVIFKKLGSSETISLTPIAASSSKAFDYMLNDYVKDLNKKNE
                     EKWGVKNTFIISTNTGNGLRGSIGMGDELREVAVIQNPYNDIAYVLDSNIYKSPFFEY
                     VLNNLKPQSKIKY"
     gene            complement(58745..60181)
                     /locus_tag="PKI16_00285"
     CDS             complement(58745..60181)
                     /locus_tag="PKI16_00285"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNR52918.1"
                     /translation="MAKEILNKPGVYKFLDKNKKVLYIGKAVNLKQRVSSYFNGNLED
                     RPWVAKMIPLVRDIETIVTENELEALVLESALIKKYKPKYNSDLKDDKSYSWIHISTN
                     EEFPKLKVVRNKDLNTIKRGRLFGPYPSGSSVKRVFTYLRKIYPFCTCTKAKKECLYF
                     YLGLCPGPYQGHISKEDYRKNIDGIIKFLTGKRTSFIKKLDKDMKEYSSKKQYEKAAE
                     LRDKIADLKYLGSSLTGDFFEDEKSYELRKESLYKKVFSELRKELKLTKLERIECYDI
                     SNIQGQEAYGSMVVNENGEIKNNEYRIFRIKESNTPNDPVMLKEVLTRRFKNEKMSKH
                     PDVVLIDGGKGQLSVVASAVPRGVLLLGISKGKRLKRKGFKPKDEFWVHREGETSRIY
                     IRNEKLLINLRDEAHRFAITYHKKARIKKGLRSQLLKIEGIGESRAKKLLMKFKSMEN
                     IKNAKLEEIDSVIKNKKVSEEVIKSLST"
     gene            complement(60163..63552)
                     /gene="dnaE"
                     /locus_tag="PKI16_00290"
     CDS             complement(60163..63552)
                     /gene="dnaE"
                     /locus_tag="PKI16_00290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNR52919.1"
                     /translation="MFTHLHLHTEYSLLDATIRITDLVEKVKSSGMTSCAITDHGSMY
                     GVFKFYTAMKEAGLKPIIGCEIYVAPRGMNQKEFGIDNKYFHLILLAKNLKGYKNLMK
                     IVSVAHMDGFYYRPRIDFETLSKYTEGIISLSACLAGPLSTALLQDNYDLALQRAKSY
                     AKIFKDNFYIEIQRNGMDEQEIANKGLMKIAKELKLPIVATCDSHYLNKEDAEIQEIL
                     WCISDGNTMDDPNRRTMPTNEFYVKTPEEMKILFKDLPEAITNTQKIVALIEDYDITF
                     GRVEPHFLDLPEGKTQKEYLKELTYDGLKDKYTKIDKELTDRVDYELKIIDDKGYNDY
                     FLVVRDFVAFCRKNGIVVGMRGSGCGSVVAYAIEITDIEPIGWELYFERFLNPERPSP
                     PDFDIDIADKRRDEVIQYAIEKYGEENVKQIGTFSKLRTRQAIRDVSRVTGVDLEIAD
                     QLSKMVEILFGKAKDIDYMIEHNPEFAEIINSSEKTRHLAEVVRKVSGLCRGVSTHAC
                     GVIITPDPVVNYAPIQRDAHGGGLGMTQYEMFDVEPIGLMKFDFLGLRNLNIIGSTIK
                     KIERSKGETIDLMKLDLHDKKTFELIQSGHTIGVFQLESEGMKRTIRSLKPETLEDLC
                     YIIAAYRPGPMQFISEYADVKEGKKKPEYIFDELEPVLKVTNGVITYQEQVIKIAQVV
                     AGYSLGKADVLRRAMGKKKIEVMEAEKPKFVEGAKERGFDAKKVELLWEKLLLFANYG
                     FNKAHSASYATIAYRTAYLKAHFPIQFMASLLEGDLDNFDRVIKDLNECERLGIDVLP
                     PTINKSGYYFEVEDDVNIRFGLGGIKNVGEDIIKNIVNERKANGNFEHLDDFVHRMSD
                     KITKRAIEYLVMSGTMDEFGDRNALLNIVPTVFEKFKSASRSIAAGQIDIFSLDESKD
                     KKSKIGKTPLPNIEKTPVKQALEWEKELLGIYFTSHPLDTLQEFFESKNCISLREALE
                     KKNHELVILGVMVNKIRKITTRKGEVMAFLMIEDKTGSSDAVVFPRIYQEIKENLVEN
                     KPILIAAKINVRDGDKSLVVEKARYVEDDKRTTSFDGVTFRIKPIHKEKDIALLKKYI
                     SSSDGDTAVRIIINDGKSKRAVLLKKKIALNSETKKWLKKF"
     gene            63589..64308
                     /locus_tag="PKI16_00295"
     CDS             63589..64308
                     /locus_tag="PKI16_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52920.1"
                     /translation="MPEEITSKINSLLKVSLHNSNIIRIGVLVIILSLYYILLSPFVE
                     NKLVDLFAGMIREADKSEVNTLTPVFDIHVYAGQYNLKTQLETGNYTGSSKFFTLDPR
                     ILAMNKFLTDYNSPMASSAQAFITEADKYGLDWRLVASISGVESAFGNLIPSNSNNAW
                     GWRGINKNARGWSMFTTWEAGIAEVTRGLAQGYGVTLSPFDIEPVYCPPCGQNPEHAW
                     ANGVTRFMNQLQYYTNNLELF"
     gene            64325..64612
                     /locus_tag="PKI16_00300"
     CDS             64325..64612
                     /locus_tag="PKI16_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52921.1"
                     /translation="MLKLIFRLAYTIILFIEALIMTRIVLILINANTGNPFAGWVKNT
                     SEMFISPFVGITANNLQIDNFVLPLTPIIALVFYIIAAFILSELLKSFSRE"
     gene            64639..65373
                     /locus_tag="PKI16_00305"
     CDS             64639..65373
                     /locus_tag="PKI16_00305"
                     /inference="COORDINATES: protein motif:HMM:NF018288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HNR52922.1"
                     /translation="MAKIDLKKTSGFQLFYDGENLQSKDLSFEGKIEISIADVRPQLL
                     NKELTCPDIFYTKYKNLDQEDLYRSKGIRINFYLLQPNLAGIEFVKTRATRVLKYPRI
                     IDVVYGGATILLQRYRNPKDNRIIKVVAKKEQKVIIPAGYAFVIVNTRQNTNLIFAEY
                     LSTKAISKVVLDEMNGLAYYIIRKNAKQETVRNPYYKIVNEPEKLDWDKILFKYGITP
                     KTPVIKQIMRKYEKFEWLFKEDSVDI"
     gene            65327..67210
                     /locus_tag="PKI16_00310"
     CDS             65327..67210
                     /locus_tag="PKI16_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNR52923.1"
                     /translation="MKNLNGYLKKIPWIFSFVLFLFLFPNSSYAVEFFTTTSSFKHTL
                     TQESVNTELVLDISSKETRVVSFFTASIPIKNLDVKCYNYKTNKKIDCTTYDRGSSTE
                     ILFNLNNAVVRPTVPLQVKVTYSTPSSDSNSYQISSYVSDIKTVKVLVIYPKEKGQPI
                     WSSDPITDIKSVGSNYQIQINNPLYSTLSLLFGNNIIYQFEVHRVFTNSLTDQNQTFE
                     LILPPDTQNQSIIWEKIEPLPNTSQLDVNGNYIFKYIVTPDSTIDATVKGHIYKRDTT
                     ASDSLPATFYTARIGYWNLTNSNEYKRINTFITKKGLSIPEDVTSVSTLENSQRELLY
                     KYLYQYTIAKLDYNRDLQLGITEQTRVGVNALVDNPSDSSPIDYADFLIALYRGYDIP
                     ARMVVGYVSNISGFTSDGFFHYWVEYYDSSLKLWITLDPFLEDYSTKSLYKSNFADHI
                     TILRRGKSPLAPNLTFYEANDFLVTSVPNAQIVPQFKVDAGLTFEKNTVTRPFTKGYI
                     SLSNTGNIAITGYEIKKSNIENITRYIDPINNMSSQIVLPKQNSNIQINLPMQEKDIN
                     FFVNIRFTNQNIFSSEEILEYEMTPTVPILLDIFVKILSTILFILLSFLIYFGIVKLK
                     KHG"
     gene            67203..67619
                     /locus_tag="PKI16_00315"
     CDS             67203..67619
                     /locus_tag="PKI16_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52924.1"
                     /translation="MDRSVIITIVILVALCLLFLLAAINNRKKTFKKRARMLRDLQDL
                     KVGAYSSNPSERITAIIKLDNLLAKALQYRFSNEKNCGENLKLARKLFSRPIYEKIWQ
                     IHKLRNQVVHDDISISEEKMQEAYRIYNLTINKILK"
     gene            67616..68173
                     /locus_tag="PKI16_00320"
     CDS             67616..68173
                     /locus_tag="PKI16_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52925.1"
                     /translation="MKKIFLIIALLFLPFNLFAQEENVSVQQNNQYFNLKLTQVSQSV
                     FDKSIKYSLEVTPLKDSAKTQILWEAPLSFTVTPKHKDFVSMAVGQTYIYEAIVHPTR
                     GGVYEITASVTAWEYDTNYTNTVSQTLTLNDSLVSQPISSDYQVGVILFTVLILLVSG
                     LAIWGAVKLTKIIMVRTKKWLTPPS"
     gene            complement(68170..68373)
                     /locus_tag="PKI16_00325"
     CDS             complement(68170..68373)
                     /locus_tag="PKI16_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNR52926.1"
                     /translation="MNVKLFSKKLRMAREAKGYSQKQLGMRVGLSDKSISMYEKGTVY
                     PPISNLFKISEALGKDICYFLKD"
     gene            complement(68378..68935)
                     /locus_tag="PKI16_00330"
     CDS             complement(68378..68935)
                     /locus_tag="PKI16_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52927.1"
                     /translation="MKNVDVPMWDKGDSTDSYSKGGGSMFSDFESLSDKIPMIEEVYE
                     KVLGRKPSSRELSLYKYSVLKRDEITRKLLNSEEHKEIVEKGRAYSELEERERLAKSN
                     ILKLKHGIEDMVVEHDELKNILEEKNKIIEKLREEKTAPYISGEIPQDRYNTYYNSTE
                     GINKGYAPFKKENFIDKLYNFLKSL"
     gene            complement(68975..69051)
                     /locus_tag="PKI16_00335"
     tRNA            complement(68975..69051)
                     /locus_tag="PKI16_00335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69015..69017),aa:Gly,seq:tcc)
     gene            complement(69072..69482)
                     /gene="rplS"
                     /locus_tag="PKI16_00340"
     CDS             complement(69072..69482)
                     /gene="rplS"
                     /locus_tag="PKI16_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNR52928.1"
                     /translation="MDNNIFKKIEEEQLKKRPDVRVGDTVKLHIKVKEGNKERVQIFE
                     GTVIAIKGDGLNKSLTVRKISYGIGVEKIVQLHSPILSKIEVVKRGSVSKSKLYYLRE
                     RVGKRALKINKIQDMYETDEVEKVKEEVVEEKAE"
     gene            69573..71102
                     /gene="gpmI"
                     /locus_tag="PKI16_00345"
     CDS             69573..71102
                     /gene="gpmI"
                     /locus_tag="PKI16_00345"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNR52929.1"
                     /translation="MNVKQKVLLIVLDGLGAAPASEGNAVVMANPEKLSALWSTYPHT
                     YLLASGEAVGLPQNVRGNSEVGHLNLGAGNTVMQTLPRINKAIEKGLLDSNNTLTEAV
                     IHAKRYESKIHLIGLLSDGSVHSHIDHFKAVIDFLSKTNLENEVYIHAMTDGRDSPPQ
                     SALTYLLDIDKHCIERGIGKIGTLIGRYYAMDRNNKWDRTQDAYFLLQNNTGERFNTY
                     QEAISTYYQQKLTDEFFPATVINDSKIEKSDVVIFLNFRPDRSLQLARAIADDNFVNF
                     QRIKPQNLFFASMVEYYKDFPNKILFPKQYLNLPLGKIVDSFGLRQLRIAESEKFPHV
                     TYFFNGGTSAIYNGEDRIMIPSPNVATYDLKPEMSAPELTDILIKRVSANMYDFILVN
                     YANPDMVGHTGNLAAGIKAVSTVDSLVYELVQNFISTGGAVIITADHGNVEEMINLDT
                     KAIDTEHSLNPVPCIIAGTDITQRNLPYGALKDIAPTILEIMGITKPAEMNGQSLIRK
                     I"
     gene            complement(71099..71719)
                     /locus_tag="PKI16_00350"
     CDS             complement(71099..71719)
                     /locus_tag="PKI16_00350"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNR52930.1"
                     /translation="MVSTIIDWLVNAISTLGYPGVAIAMFLESFFAPIPSEIILPFSG
                     FVCARDGMNIYVTIIVASLAAYLGSLPFYFIGKWGKEGVDRFLRRFGKYMFIPASDLD
                     KGYKAFDRYGNGIVFFGRLIPIVRTVISFSAGVAKMNFGIFSLYTLLGSLLWSSVLAF
                     AGFLLGENWKIVEGYVNKYENVIYVVLGVVVVTYIVRGIYKLVKKN"
     gene            complement(71726..72265)
                     /locus_tag="PKI16_00355"
     CDS             complement(71726..72265)
                     /locus_tag="PKI16_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNR52931.1"
                     /translation="MSNQLKLLLYIIFIAGIFYFLQNKFDLFDISFVEEGKISDIFKS
                     DKGEANIEGTLRDMEISLGDGKTVNLRVEIADSPEERELGLSHRTYLGDYEGMYFVFE
                     QNTNSSFWMKDMFIPLDLIFVDENGFVVDIKEGLQPCTDICPSIFSGTTYRYVLEVNS
                     GFCSTNGVKIGNGVQVNLK"
     gene            72336..74507
                     /locus_tag="PKI16_00360"
     CDS             72336..74507
                     /locus_tag="PKI16_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNR52932.1"
                     /translation="MQTFKKYLNPLYKQLEDIGSFSDLTDLSALEQEEYPTLQEVTKK
                     KIASIRSLLPRLDRLEEELEHQITLIETESDIDERKDAKNVYNQILKQIEALIGRINN
                     ELIALDSPYFGKIVFLPDDSKTGKPLLLYIGKFALVDEKTHVPIITDWRSPIANIYYE
                     NSGPTKNVSFQAPVGKRTGELKQKRQFQIARARIRGIYDAKSGNVAADEFLLAQLNER
                     LGKKLQDIVSTIQAQQNEIIREDINHPSVIQGVAGSGKTTILLHRLAYLFYTYKETIT
                     SENSLIIAPNQMFIDYVSDVLPDLGISKVDTQTYLFWAKSFLTWGDNYRLSNLEEDMK
                     IKEFKGSLEFLDILDAYFDKFEKELLEEMPYSRKDIVANRYFLLKKNYLDIDMAERLE
                     LALEYSFAQKHFRKSLDGYIGDMNDNDFDIKKKVLTYFRNNTSVYRLYRNLFKDTLLN
                     QDVCTYSLNGLVSEGKNNFFRIEDLAPMVYLYQKINGIKNVTRDTVMVDEAQDMSYIQ
                     LATLTKIAKDGNITLAGDLAQSIIPPFYIRSWDGVLDILERYTHKKVSFHQLQRCYRT
                     TVEIIDYANGLFREKFPKSYQLPEAVLRHGDDVEILNIGSTLDSCSEKELKVVVEKIK
                     EQFEKGAVTCSVLCRDKNHSTNIYNRLKSYNEYLDREVINYTENDYKQGLLVMPIENA
                     KGLEFDSVFIADMDSYPDDELSWRLLYVGITRALHRLFIIK"
     gene            74558..75130
                     /locus_tag="PKI16_00365"
     CDS             74558..75130
                     /locus_tag="PKI16_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52933.1"
                     /translation="MPKKKLARIISEMFNGFFTMLLTPILVFVVSDIPTIYKIIFSIL
                     YISAPVGTFFILKKLGKVTDYEFTKREERPLFFIIVTISFLILFIVSTFILKNPTIST
                     VTVASFTASTILTIVSLFWKMSGHMTYSTLLFFTCIFLFPNLSFLWLLFLFTPAIAWS
                     RVELGKHTWMQTVAGVLVSTAISITFFFVL"
     gene            75147..76589
                     /gene="gatA"
                     /locus_tag="PKI16_00370"
     CDS             75147..76589
                     /gene="gatA"
                     /locus_tag="PKI16_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNR52934.1"
                     /translation="MENLSITQLREKLLNKDISATEAVNYYLDRIARLEKTLNAYITV
                     NKEDALKKAKDFDNNFDKLKDKKLSGIPISVKDVFSTKDILTTCASHILDGYKPVYNA
                     TVVQRILDEQAIILGKTNLDQFCHGSGTVTSYYGPTRNPWDKETLPGGSSGGSAAAVA
                     ADMCTASVGTETAGSIRLPASWCGIVGLKPTYSRVSRYGVLAMGSSLDSPGPIVKTVE
                     DAAYLLSIIAGYDKNDFTSSKEPVNDYYKLLDPSKIKGMRLALPKEYLDLDIQDGVRK
                     NFESSISILKDLGAIIEEVSVMDPKFAMAVYTITCRSEVSSNLARYDGTRYGLEGSKN
                     ENVKEYYESTRGEGFGEEPKRRVMTGTFSLSAGYADEYFKKSEQVRQLIKDDLDGVLK
                     KYDAIVSPSTPSVALKDEDADNPLFGEIADVLAEGSSEAGLPGISIPSGLSNRMPTGI
                     QFIGRYFDEQTILNAAYALEKEIGTLKLRL"
     gene            complement(76578..77471)
                     /locus_tag="PKI16_00375"
     CDS             complement(76578..77471)
                     /locus_tag="PKI16_00375"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNR52935.1"
                     /translation="MKRKYIILFIIAILLVILIPLVYIFRDQILEYIPIFNKQGNTQE
                     EDKKIVTKSTKGVEYRMVTPLPNSEVDCTFDIEGEIPGSWFFEGVFPITLVSDAGAEI
                     LTVQAKAVGDSYTDEFVKFTAEVTCTDICNGDAKLVFIKDNPSGEVANDDSFEIPIFF
                     KELCDIDSTMSLLVYFGNTMKDPNSENCEKVYAVSRKVVKTEAVGRAALLELLKGTTS
                     AEEDKGYISSIPAGVTINSLKISKGVAYVDFNEKLGEGVGGACLVERIRGQITQTLKQ
                     FSTVDKVVISINGESEDILQP"
     gene            complement(77528..77601)
                     /locus_tag="PKI16_00380"
     tRNA            complement(77528..77601)
                     /locus_tag="PKI16_00380"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77565..77567),aa:Lys,seq:ttt)
     gene            complement(77657..78223)
                     /locus_tag="PKI16_00385"
     CDS             complement(77657..78223)
                     /locus_tag="PKI16_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52936.1"
                     /translation="MIEDVENKISDGTRLEVARENLEFFGELMPPNSVLTINLADYQD
                     RARFSVNHICNSLKEALEIPSIRSINISKSSIILYDDDGMKGLFLANRVPDKQSRIGL
                     PGFIPDNSRSYYGIIWGKEASPRIYSSFGNELFEKESHIMEEKDVSAVHSLSYTLSDF
                     ALKITTDPVTHKRKHELLGIRTRSIDSY"
     gene            complement(78285..78923)
                     /locus_tag="PKI16_00390"
     CDS             complement(78285..78923)
                     /locus_tag="PKI16_00390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNR52937.1"
                     /translation="MIIYPDTKLEEKLWSQGYQIVAGLDEAGRGPLAGPVVAGCVVIN
                     SLDQVVPFVRDSKKMTKKKREEAFSLITERSFAFGYGIVSAADIDRLGIQKAVLEAMS
                     IALQVVEEKVGKRVDYIIADGMNISSIPNYKMNKITQGDLFHYSISASSVVAKVVRDR
                     IMYDYAKKYPIYGFERHVGYGTKSHMDVLKKHGPCIIHRRSFKPIDRLIYKG"
     gene            78929..79099
                     /locus_tag="PKI16_00395"
     CDS             78929..79099
                     /locus_tag="PKI16_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52938.1"
                     /translation="MIPLSMDEVTSTTTLSEQLQEQPVSSTTSLGSVLIASLSISLFL
                     VIGYLLIKWFRF"
     gene            79132..79500
                     /locus_tag="PKI16_00400"
     CDS             79132..79500
                     /locus_tag="PKI16_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNR52939.1"
                     /translation="MSEDKTFSFLKGILVGAIAGAVTGVLLAPKSGVETREDLKKLAV
                     DFGDKANELYTKASTILKEKLTSLKKAGKQINRESYLELVSQVVDEIKKDGLVTEEVA
                     QKISAQLKTDWALVKEELKK"
     gene            79587..79663
                     /locus_tag="PKI16_00405"
     tRNA            79587..79663
                     /locus_tag="PKI16_00405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:79621..79623,aa:Gly,seq:ccc)
     gene            complement(79670..79921)
                     /locus_tag="PKI16_00410"
     CDS             complement(79670..79921)
                     /locus_tag="PKI16_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNR52940.1"
                     /translation="MEYRFTVSAVRDIKKLPKSIQKRIIIKLDFFCKQEDPLLFAKPL
                     TDYRTGSFRFRVGEYRVIFDVNDDEIKILRIGHRREVYR"
     gene            complement(79927..80202)
                     /locus_tag="PKI16_00415"
     CDS             complement(79927..80202)
                     /locus_tag="PKI16_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52941.1"
                     /translation="MRQLISSEKFTNIQKAQAGLTRLFEDAADTNTFYRVLKNDKALG
                     VLIPNDMWEDIIEDLEAMASPILKERIDKRRKEKTYSANEVKKMLNL"
     gene            80302..80374
                     /locus_tag="PKI16_00420"
     tRNA            80302..80374
                     /locus_tag="PKI16_00420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80335..80337,aa:Thr,seq:cgt)
     gene            80385..81455
                     /locus_tag="PKI16_00425"
     CDS             80385..81455
                     /locus_tag="PKI16_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52942.1"
                     /translation="MKTPTYTITNLILSYIVKYELAIKDITHYPLPEKYLLPLQEKYG
                     AEDLNALGDLVGNTIGYNKSLQVQRGQELASQRKNLQLFINIRNAKDYINSYTTNSSL
                     KPSIELSSHLNKLAMKGLVDDWDLAKLKTFSEKPNEIYDTWFKMRDYYPHIDSASHFN
                     EIFEWIKDGKDSNHKLIKFAALTYEFIDKAPYFVGNQITAILTLEILAKQYGYNPDNL
                     LPFFKSINNISQDLLDAFKISKSKRDLTTFIEAFLYAISLTAIDVSRELKDTYTKKVK
                     KQGALEISLNPRQIKVMDYITLNHKITRSKYTKMMGVSFMTSYRDLQEMVDKGYIKQK
                     GQGRGTFYVLSKEQEDIVLEIE"
     gene            81485..82951
                     /gene="gatB"
                     /locus_tag="PKI16_00430"
     CDS             81485..82951
                     /gene="gatB"
                     /locus_tag="PKI16_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNR52943.1"
                     /translation="MEYDIIVGLEIHVQTKTESKMFCSCPTGYFQKDPNTHVCPVCLG
                     LPGALPVPNRRAIELCILMGLATNCKINQNIYFDRKHYFYPDLPKGYQISQYKDPICS
                     NGKVTLPSGVEIEIERIHQEEDVAKSTHHKEVSTNKDYSLIDYNKSGIPLIEIVTKPC
                     IRSAKDAKDFATQIRQICRYLNISDADMEKGQMRCEPNISIQEKGKWKYEDGKILPVG
                     DYKLNNKVEVKNIGSITAVEKSIEYEVKRLTEEITNGKEIIQQTRGWNADKGITEFQR
                     SKETADDYRYMPEPDIPVIEVSKKDIERISNELVELPHEKITRYTKDYDISTYAAEVI
                     SSTKENASYFESLLAELKPSLDEKTAAKEAANWLMGTVYSIGKDVDIKDLALLIKKFR
                     DGIITKGVAEELLKNCLENNKDISDELEKFESSSKENSEKLEDIVKDIIAKNPKAVAD
                     YKGGKESTIGYLVGQVMQSTKGSANPNEAKNILVKLLK"
     gene            82948..83241
                     /gene="gatC"
                     /locus_tag="PKI16_00435"
     CDS             82948..83241
                     /gene="gatC"
                     /locus_tag="PKI16_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNR52944.1"
                     /translation="MKIDVKHIADLSRIALTQKELDKFTPQMETILQSASELKNVDTD
                     NMAPMKKHRPFSDLRDDIPTPSLTQEEVLKNAKHTEKGYVKVYGKIFGDIEES"
     gene            83270..83358
                     /locus_tag="PKI16_00440"
     tRNA            83270..83358
                     /locus_tag="PKI16_00440"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83304..83306,aa:Leu,seq:tag)
     gene            83380..83619
                     /locus_tag="PKI16_00445"
     CDS             83380..83619
                     /locus_tag="PKI16_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52945.1"
                     /translation="MLKKIIKPFQEVLLERKLCVGCTGQLENSKRLGNLSDNRFMVQC
                     KCKRRYVFDKEMNKYQRATFAEEQQILKQIKKDSE"
     gene            83666..83741
                     /locus_tag="PKI16_00450"
     tRNA            83666..83741
                     /locus_tag="PKI16_00450"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83700..83702,aa:Glu,seq:ttc)
     gene            83764..84372
                     /gene="lepB"
                     /locus_tag="PKI16_00455"
     CDS             83764..84372
                     /gene="lepB"
                     /locus_tag="PKI16_00455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNR52946.1"
                     /translation="MYETETKIKTPTEKIAATFSSIGQFLLSFLETIVVALVISIVLY
                     LFIMTPHEVIGNSMHPTYQNGEYLMANKLLYRFQDPQRGDVIIFKYSDTQDFIKRVIG
                     LPGDKVMLKDGHIYINDQLLDESAYLSNSIYTNGGEFLHEGESITVPDNEYFVCGDNR
                     PHSSDSRTFGPVSKDNIKGKAWLVYFPFSQFRIAQHETYSNL"
     gene            84411..85205
                     /locus_tag="PKI16_00460"
     CDS             84411..85205
                     /locus_tag="PKI16_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR52947.1"
                     /translation="MKTFVIANQKGGVGKTTTAINLGASLALKKKKVLLIDLDPQSNL
                     SSGIGFTQQFDKQNWSSSDQPPYKSIYDVLVQDDPLSSVFVATSTPNLFLVPSHLSLA
                     GAEIEMVNMLSRETLLKKALSKFTEDFDYAIIDCPPSLGLLTINALIAAEKLLIPIQC
                     EYFALEGLGQLLETTKLIKGINPSLTIGGVILTMYDSRTRLSGSVVADVQSFFGDVAF
                     NSIVPRNVRLSEAPSYGQTIFQYDKKSTGALAYKSLASEFIQRFDD"
     gene            85223..86077
                     /locus_tag="PKI16_00465"
     CDS             85223..86077
                     /locus_tag="PKI16_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNR52948.1"
                     /translation="MQNNERLGKGLAALISTNEIDNTNSAYNPNFDINKIEANPYQPR
                     MHIDPEELIDIADSIREHGVIQPLIITKDKDSDKYFLIAGERRLRASQLAGLKTVPVV
                     IQESSPQDMLELAIIENIQRKDLNPLEEAYAFKKMQEEFGIPQRDIARKVGLSRVAIT
                     NKIRLLTIPNEVKEDVLNEKLSEGHARALLGIKDKVSLIAAADLVVKRGMSVRETEAL
                     VRKINFGKGSTTRNWKRSDEETDNYSRLLSKKLGYTAHISKMTKGGKVVIRYNTHKEL
                     KELMSKLL"
     gene            complement(86074..86820)
                     /locus_tag="PKI16_00470"
     CDS             complement(86074..86820)
                     /locus_tag="PKI16_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR52949.1"
                     /translation="MSKTRFIQVESKNAILELLKEGRDFDKLYIAANAFKDEKVREIV
                     EIAGKKNIPVLKVSRKIITRRLRNSNSESILGYMYSQNNWDLEELLDKLSSEGKQPFF
                     LVLDDLKYTQNIAAIMRTAFAVGVNGIIVNPQEKNLITNETIRISMGAAERIPLVETN
                     LFNALKLLTKNNIKIFAVHMDGKVYFETNLKGAVAFVMGAEDVGITAGLLERADEKIS
                     IPMREGIGSLNVSASAAVVMYEKLRQEVTA"
     gene            complement(86834..87100)
                     /locus_tag="PKI16_00475"
     CDS             complement(86834..87100)
                     /locus_tag="PKI16_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNR52950.1"
                     /translation="MSHVVGAASTSNNGHVAGKRLGVKKFAGEVVKNGNIIVKQRGSV
                     YHSGKNTYMSKDYSIHAHKDGVVQFRRMTGFKRGQYYVDVVESV"
     gene            complement(87133..88320)
                     /locus_tag="PKI16_00480"
     CDS             complement(87133..88320)
                     /locus_tag="PKI16_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNR52951.1"
                     /translation="MPKQYYFESGNFQDLSFAELVSVFNTFGLNKDTIHRYSNNILLV
                     KSNDISEEVLKKVFNRLGGFIRFGYIIEDLDTFLNDFSKKEDKVIFGLSILGNPESTD
                     SRFLRKLGKQIKVGLKEVGRPSRFVNPLSRSSALNSAQIVGNSILEKGFELCIIRTEN
                     EEIYGSTISVQDVDGFAKRDMDKPHTDLEMGTLPPKLARIMVNLTGRKDGIVWDPFCG
                     SGTILMEAGILGFDLLGSDIDEKAIEYTEKNIEWLSGIGEIGDVKYKVFQMDVTNPPA
                     RIVSDLKKTEVKCVVCEPYMGPPQRRVLFPNRADVYLERVKALYLGLIDILNRFAHEG
                     FVVVVIIPSYKTHTGWKTFSIRDIFDKKWDVVNGELCKGRDLMWKRKNSIITRNIFVL
                     RRR"
     gene            complement(88320..88718)
                     /gene="rplU"
                     /locus_tag="PKI16_00485"
     CDS             complement(88320..88718)
                     /gene="rplU"
                     /locus_tag="PKI16_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNR52952.1"
                     /translation="MAEIEKEIKKTPKNVTKRVVKEETKKVSKDKFAVIAISGVQLKV
                     YEGKEYEINKLVGNKGDKIEISEVLLIADGEDIKIGNPYIEGSKVSLEISSQKKGEKI
                     NGLKYKAKSRYRKHFGSRPLITKVLVKKIG"
     gene            complement(88761..90086)
                     /locus_tag="PKI16_00490"
     CDS             complement(88761..90086)
                     /locus_tag="PKI16_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNR52953.1"
                     /translation="MPINISKKRTFFGSNIVIVLPLILLSALGVITLLSTRILPDGSL
                     GELSIVWKQVIALGIGWLGFLFFSKVDLTYLKYWQVLALIYGVTVALLVITLFFAPVI
                     NGVRRWLVIGGFQIQPSEIAKLSVILITASIMSMKEKFNEWILFLITLASTLVIFLLI
                     YLEPGGSMSLLIMSIWFLLAFLGLSNPFRNSVLVTIIGSITAGFLVAAITGNWYWYAL
                     VILGIVIAIFSIYSRRMSKILVVISLSVALLLGVLLSVVWDSVLRDYQKDRIEAFFNP
                     AETESNIGFNVNQSRIAIGSGQIFGKGFGNGTQSKREFIPEHETDFIFASYAEEFGLV
                     GSLFLLGLYAAIVLVCFITGMNLVEDRMLSLICIGVGLQILLGVFINVGTNLGAIPAT
                     GIPLPLMSSGGTVTIMTLFSMGIVQNIYMKYLRKTKNKSEDILDVYEIS"
     gene            complement(90103..91335)
                     /gene="ftsZ"
                     /locus_tag="PKI16_00495"
     CDS             complement(90103..91335)
                     /gene="ftsZ"
                     /locus_tag="PKI16_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019391732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNR52954.1"
                     /translation="MLVTTNVDPVAKIKVVGVGGGGCNAINTMMNDYDISGVEFMAFN
                     TDAQALKMSSAPVTLQLGEDLTHGLGVGGNHEMGAQSAEESLDQIQELLEGANMVFIT
                     AGMGGGTGTGAAPIIAGVAKNMGALTVAVVTKPFDFEGNKRKEIAEEGIAALRDKVDT
                     LIVVPNQRLLEIIDESEKLSFIDAMKKVDSVLAEGVRSISSLISETGYINADFNDAKS
                     VMQNAGTALMGIGRASGENRAAEAARKATTSPLLDMSIEGATGVLYNVVGADLTLSEI
                     SQVSELIKEAVDPSANIKFGAQIDPDAGDELTITVVATGFDENKQVYTKLQKTTGQPL
                     RTFGFKEEEKKEDIDDDEDILDNKDEVLETFERDDDEKEEHDDESVKKSEATINIPQE
                     DGEDPLDIPAFMRRQDKD"
     gene            complement(91359..92654)
                     /gene="ftsA"
                     /locus_tag="PKI16_00500"
     CDS             complement(91359..92654)
                     /gene="ftsA"
                     /locus_tag="PKI16_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNR52955.1"
                     /translation="MARKIITAIDVGSSKITTVISSVEDNKTPSVIGVCTYPSKGIKK
                     GVVVNIDDATNAISESVTAAERMAGVTISDVYVSINGELVTSLNNKGVVAVAGSEITI
                     DDTYRAIENARTLSLPDNLNPIHIIPREFVVDNQGGIKYPIGMSGGRLEVETHIITAP
                     NSYWQNIKKCVEQIGLHVYDVIFNGWASSLSVLTDTEKELGVTLMDIGAGTTCIAIFE
                     EGAIIYSSSIPLGGNSITSDIAIGLQVSLEDAEKIKVNMSDILDNKCNVKKGKDMDST
                     PALLRRADEPVEKKTKSDEVDLSDFGVEKSVSKEMIQKIVEARCEEIFEMAKENVSKA
                     GYDIAMPAGVVLTGGSALLRNITKLTQSVFGVAARVGYPSGLSGMTEEISDPSFACVQ
                     GLIKHALDDDVDISGNEKGKVNVNGFFEKVGEWFKSLLP"
     gene            complement(92763..93569)
                     /locus_tag="PKI16_00505"
     CDS             complement(92763..93569)
                     /locus_tag="PKI16_00505"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNR52956.1"
                     /translation="MEITKQPTNHILDKVSRFKPVAFIRKNLFVTIVILLIIVTNLVG
                     IWNIKKYEIYDISGAEIPQNILNDISKYIEENSVGENFFLLSPSELNSQLVSNISYIK
                     SADVKKSLPNKLEIFVEIYQPELLSVLNGDQCYVLSNEGIMLERVCEGETRDCCMNYI
                     ETNKLYLLTSSSVDISKLENGKEKLLVMESISKVTKVTEALGYNIKSVTLSGDILELQ
                     TIDGKILRFSLGEDLDTQLERLVIVAGKIKSDKIKFKSLDVRFERPVIKN"
     gene            93702..94259
                     /locus_tag="PKI16_00510"
     CDS             93702..94259
                     /locus_tag="PKI16_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52957.1"
                     /translation="MNLYTFSCWGYLHPGDFGGWVHGQEIKKGISFCSYYPKDGLAFL
                     SPCGALLLENGEKITTSKVINQAYFSAKEPEDFPTRHICRKHCKKILRTLTKEYADVK
                     NLLVLVTFEDELFLTFEDVLTRLRVKEDSIVNYEANQIVTGNGRSEDYYVRYVIFQMP
                     NKHRTGIISNGKYLYNDNGVLKVLN"
BASE COUNT        31160 a        16374 c        16144 g        30739 t
ORIGIN      
        1 gcaactcctg ttcaatattc ttcagaatat aatatatcta caccaggttt agcaggtgga
       61 gtgtttgatg ataatgcaac tgtagacatc acttactttg gaaaagatta tgtttgtggt
      121 actgtaacaa gtgatgatgg agattctagt atcaaggggg aatttattgc taaatatgta
      181 gacaaacttt aattattatt taatatggca aaaccaacaa aggatgcacc tttaatttgt
      241 acgatttgta caatacttgc actaattgga atagtggctg ggatagtgag tcacaatccc
      301 ctattcacca tattcttctt actaccaact gcgatatatg aggtttacag aacagagggt
      361 gaatctacta gagcctcttc cataatcctt ctactagtat taattgctga gatattcctg
      421 ctagtgttca atattaactt caacttagct gaatatcttg gtacaacaga aaaaacaatt
      481 ggaggatatc ttgttccttt gggagatatt aaaatcgttg gacccactgt tatggctgta
      541 cttgccgtta ttctcttctt aaggacatat ggcaagttta caaaatggtt agctgtagta
      601 atttttgtta ctgcgtttgc aattatatat acaattgatc cagtaatatt taaagatctt
      661 tttaaatatg ctgtacaaca gggtgtagat agtctgtaat atgttacaat ggagatatat
      721 aaattaagta ttttatattt atgggatata tagataacat tgagaaggaa acattagaga
      781 atagtaattt cagaaaagtt ttgtttactg gtgggcatat gcaattagta gtcatgtctc
      841 ttaagccagg tgaagatatt ggagaagagg tacacaatac tgttgaccaa ttctttagat
      901 ttgaaagtgg tgaaggcaag gttgttatga atggagaaga aagtatcgtt ggtgctgaaa
      961 tggttgccat tgtgcctgca ggaactcaac ataacatcat aaatacatct gaaacagaag
     1021 atttaaagct ttacacaata tatgcgccag caaaccatcc tgagggaaca gtccatgcta
     1081 caaaggaaga agctatggcc gcagaggaag agcatcacca gtagtattag gagttttccg
     1141 atattggtga tataatcttc cgtttgatgt agatctaaac gaaataatct gccacatata
     1201 aatatccata aggctaaata tatttttaat ttcggttatc tctgcgtaga cgaaattaaa
     1261 atatataaat aagctaatgg aatactattc cagaaataga aaccgtaaaa gaagatttca
     1321 acctcacaat aacagaggta gaggaaccaa tataaacgta aacagattca tagcgaaagc
     1381 tgtagagagt gaaatgccaa atatctatgt agatggacat aacttctctg aattcgatgt
     1441 tgtaggccct cttagaaaga acattcaata caagaaatat gatgtaccta ccaaaataca
     1501 gcatgaagct atacctctta tactttcagg gagagatatc ttaggtattg caagcacagg
     1561 ttctggtaaa acagctgcat tcttaattcc tttgattaac aaaatgatta aagattcaaa
     1621 ccagagatgt ttggttgttg agccaacaag agaattggct atgcaaatac aggacgagtt
     1681 catggctctt gcaagagata caggaattag gtctgcacta ataattggtg gtagtagcat
     1741 gtatgctcaa atcaatatat taagaagaga tcctcgtttt gtagtatgta cacctggtag
     1801 aatgaaagat ttaagtgata gaagagtaat agatttaaga aaaataaaca atgttgtact
     1861 agatgaagta gatagaatgt tagatatggg attcatagat gatattaaat acataacatc
     1921 taggcttaat gagaagagac agtccctatt cttctctgca acaatgaaca gaaagtctga
     1981 agatattgca aacacattac ttaaaaatcc cgctagagtt gaaattgaga cacagcaagc
     2041 aggaaagaat gttgaccaag atatcgtaag agtaagatca agagaagaga aggtagctct
     2101 gttaacagag tatcttaaaa gagatgaatt cgagaaagta ctaatcttta caagaactaa
     2161 gagggaagca gatagattat ctgacaaact gtttgaagct ggtattaaga ctggtgctct
     2221 tcatggtaac aaaggacagg gtcaaagaag tagaattatt gaaagtttta agagagggaa
     2281 tcttgctata ttaatagcta cagatatcgc ttctagagga ttggatgtaa ataatatctc
     2341 tcatgtaatc aattatgatg tacctgagaa ttttgatgat tatatccatc gtattggaag
     2401 aacaggaaga gctggaaaga aaggcatagc aattacgttt gttggatagt atcctttaaa
     2461 aacgcaaaag gatggagcac caatatacgc catccagagc aggaacgtat gcattacggc
     2521 atactgatta ggccatggta actatataaa tagaaataaa tctctattag tgtcccctgg
     2581 tgttaatgtt cgaatcatca acatatcttt caattcctca gaagtcttct atatagaaaa
     2641 attggttcta tacagggacc ggtaccaacc tgataggatt tgaaagtcct agcattttta
     2701 tagtagtgcg atatgacaaa tatgtcaata gatttcaatt gagaaaatgc tacgggatta
     2761 ataaaacgga aggacagttt tgaagatata ggcgttttaa cgatttaaag atatcgtttt
     2821 gcagaaaaac tatagcttta tatttactaa atcatttata tatttacaag cagcagatat
     2881 ttctgaagat ctaacggtta atcctggtct cctgtttacc tcaagcacat agttttttcc
     2941 agtatttgta tctgtaacaa catctacccc tgcccagtta agtttaagcg cttttgcaga
     3001 ttgtattgct aattccaaaa tgtctttaga aacattcttt acatttatga agtcttcact
     3061 agcacctagg gctacattgt ttctatattt atcttctttt ctagtccttt tgcatacgga
     3121 ctcggatttt ccattagata ttactactct gtagtcgaag gtattgggta tgaattcctg
     3181 gaatatatat ctgttgaatt taccattttc ttttatggtt tgttcaatat ctgttttctt
     3241 atcaattaaa tatacatgag aaccaagaga tcctagggag gttttgtata tacaaggtag
     3301 tttacagatt ttttcaatat cactaatgtt ttcgttattt acctttaatc cattatggaa
     3361 gaaggtatta ggaacaagta tattctttga tgctaaggag aatatgtcgg agagtttagt
     3421 attgtgtgcg tttgttttgt tatgtgggat ctttttagat ttaaggtaaa ggtgtaagag
     3481 atatgccatg tgtgttgtat tccatccaga ttgtatccag atgtaatcat atttttttaa
     3541 gtcaaaatct tttatttcta cctctatctc tccatctatt attgtaaaga tgatgtcttt
     3601 aattgaagct atatctagag tgatgtcttt aagtttgagt ttcttttgta ttgctacttt
     3661 ttcctgcgca gggatattta tgccgatggc aaggacagta cgattaatca tatttgtaca
     3721 agcggaaatt atcccaacat tatagcactt ttagagagtg attgggttct tgtgagaaat
     3781 ggtcgttaaa gcaaaagaag aagcactgta ctaaataaat agttggtttt ggttagaacc
     3841 tggctccttc tttctgttaa ctaccaccgt agtagttagg tatctaaaat atatcatttt
     3901 ggtattagtg tgtcaacagg taaaagatac ctacttacca gcgtttttct aacctatcga
     3961 atatgggatg tctagaatat gaagtatgct ctttaaattt aaccattatt aagataagat
     4021 tgtaatatga tatatacatt tattgctatc tttgttttac agataatttt ctttgtctat
     4081 gctgcattaa agaaaacaga caaagtaaca gatctctcat atggtcttac ctttataatt
     4141 gcctctatct ttgcatatgt attaaatatt gaatatgctt ccttttttaa aataatcctt
     4201 gttctccttg tatccatttg gggtattagg ttatccgttt atctgtttgt taggatatta
     4261 aaaacaggca aagataaaag atttgatgga gtaagagaag atttaaaaag atttgcatcc
     4321 ttttggttac ttcaagctat ctccatattt gttattcttc ttccaactac atacattcta
     4381 acaagtgata tggatatgtc tcttaactgg atatctttaa ttggtttaat cttggctatt
     4441 tctggaatcc taattgaagg gattgcagat tctcagaagt ttgtatttaa gaaaaaggtt
     4501 gagaacaaag gtaagtggat tgatacaggg ttatggaagt attctcgcca tcctaattat
     4561 ctaggagaga taatgatgtg ggttggtgtg tttatatatt gttttgtata tattaatggt
     4621 attggattaa tagcaatact ttctccaata tatatttctg tcctactgtt gtgggtaagt
     4681 ggtattccaa cattagagag ggagtatgac aagaggtacg tgaataataa ggagtatcaa
     4741 gcatataaga aggatacagg tgtattattt cctaagttgt tctaatcttt aggaaatact
     4801 atctggggta gattgtggga agatattaga aacggactgt ttgcagttta tttcaaaatt
     4861 tagcagaaag aaattttcga taacatatag acatctctat gttataattc actgataagc
     4921 tattacttag gtttatggta aataaaaaaa gttctatccc tccgaaaaac attcctcttt
     4981 ctgagattgg tggaatgatt aaaatagctt ttaagattag ttccactgga gttgaaataa
     5041 cttatgacaa gaatcttgat atacagaaaa tgttttttat tcttttatat ctctatacca
     5101 gtatactaat acgagcaatc aataatttag gttctgcatc tgcacaatcg tcatttattt
     5161 ctcttgatta taacccaaga aacaaacgaa tctcctataa acaaggtagt attggggaaa
     5221 aacattttac tggaacattt gaaatttctg ataatgcaaa aaatctacta caacttaatt
     5281 tggaaggttt taagtttttc tcaagcgatg cttctactga gttaattatg tcactgaaaa
     5341 agcttcaaga gtttatcata tatacatata aggatgaaaa gaaaatccat tacgcatttc
     5401 gtgtcatcta caaactgctt attcgggata atgacaattt cgaagttaca agcaaggcgt
     5461 catctatgga gcaaatgcaa atttttgatg aaattgcact ccaaatccat tacttcctag
     5521 aggatactga gaaatggttt gatacctatg gggaggaaat actgcaggac gaaccacttt
     5581 agctaattca gggtaattgt ttagtatttt acacaaggct ttcattattg aaatccttaa
     5641 cttcttgttc aatttggata aagcagtgtg ttataattca tttataatat tgaaagtttt
     5701 ctattgtgga gattagtgaa tatataaaaa agaaatacgg gaatagagaa cactctctta
     5761 ttgttacatt aaaaagatat tttagtgagg gaagtattga tgttgtaaaa ccactaaaaa
     5821 agaaatggca aaatatcttg aagacggaag attttttaga gaaaggtttt gatgagctat
     5881 atagctcgtt aatagagaca aatacaagag agaaacctgg tggattagat gagtttatta
     5941 ttaaaaaggt gtatttagct ataactattg gctttttgtt agcgataatt aataatgctg
     6001 ataaaaagaa gtttagagag cttgccacat cagggacttt acttttgtat ttacaagata
     6061 tcaaattgcc tatctttgat gagtatatgc aagagtatat agttccattc ttcgacgaga
     6121 aagcagaaaa aggactccat agtataatat tgttattagt ggatgaatta atagccaaag
     6181 attggttaga atcattgaga aattgttatt catccgttaa cttaagtata gaaaaagacc
     6241 aattgtcaga agaactgata aagtattact ttatcaaaaa agatgaattg tctgtagaga
     6301 ctgataatta tattgaaaag ctttataagt aaaggggatt tagaatcgga gcctctttct
     6361 gtaaaacata gatttccctc ttaatatttt ttgttctgtt tagttatcct tcttttcaaa
     6421 acttgttcct aatgtatatc ctattattcc agctagtatt gttataacag attctccact
     6481 aaccagtttt tcacttgcta atactataac tcccaggcta acgacaataa tggcaagtat
     6541 ttttacaaga ttatttggtg tgatgaaatg tttaagttcc cctgttgtag atttcattac
     6601 aaacatcatt actacccaag aaatagtgat agtaataaag acggaaagaa ttaacaaagc
     6661 aattatattc ataagctctt ttgtttaaca attaagttat taacaattat atcacctctc
     6721 aataattgga gatatcttcg ttatataaag gtatttatag attgtgagaa aagacttata
     6781 atcattcata tgccgaaact aactcaagtt tacaaatgcg aagacaaata cgaccaaata
     6841 ctaatcaata agtgtctatt ccttgttatg gataacaata cggaacaggc aataaaaatt
     6901 tatcaaaaag agataggtaa taagaatatt aacgccaata aatatgtaga gaacatattt
     6961 aatacaatag attcaattgt gaaagctaag catcaatgga aaatgtatca aaaactagaa
     7021 aaaaaagtaa ttaagaaaat aaaaaaggaa ataggaaaat caaagacggt atgtgaccaa
     7081 ggttttgcag aaccagaagc tatatctcat tttgatgctt atttaaccca gcttaaaaca
     7141 gcattagatt ctttagcttt ggcaattggt tctttatatg gttttcaccc agacggatgg
     7201 cataaaggaa agaatgaaaa aggtgagagg gtaagcggtc ttagactaat caatgctcta
     7261 gagaatttag gtataaagca acaaagaagt gtcgaattga tactaaatta cataaaagaa
     7321 cagattccaa ccattaataa gttggtattt ttaagagacg gctctgttca taaaaaggga
     7381 gatagaaaga gagagattac cgatttcgtt tattatcaga ataacgaagt tatacaatct
     7441 cctcttttaa taactgatga tggtaattta tttctaagta aagttatgga aaggagtata
     7501 gattattttt gtaattttac acttaccgta ataattctaa gtctttcaaa cctaacgcct
     7561 ctaatgaaac ctgttattga tgagaagaac gaaaaagtct tttggttctt taacttcaaa
     7621 taagaagaag agagaacttt taagatggat ataggtttca aaatttattg ggaggaagta
     7681 taattcaaca ttgtaggtag tgtatataaa tataaagata taaaaatgag taaaacaatc
     7741 ttcaagacta ttcagtatcc tatccagact ttaattgatg atattgacca cggaaagatt
     7801 gctttacctg atatccaaag accttttgtt tggaatacaa caaaagtgag agatttgttt
     7861 gattcaatat atagaggata tccagttggg tatttacttt tttgggagaa cataacagac
     7921 aagaatagaa aagatatagg aattgataca aaagaaagca atgctaaaaa tctgattatt
     7981 gatggacaac aaagacttac agcactcttc tccactataa aaggtattcc cataattgat
     8041 gagaactata aagagaaaag aataaaaatt gcctttaatc cattaaaaga gaagttcgaa
     8101 gtatcaaatg ttgctacgga taaagatgtt gaatatatat ctgatataag tattttattc
     8161 aaatctgatt ttagtgagta tcgctttatt aatgaataca taggtaaact aaaggagaat
     8221 caggaaatta cacctactat agaagaaatg gtttcagaga atatcaccca ccttcggtca
     8281 atacttggat acgatttcac tatactcgaa attgctgaaa atgttgatga agagaccgtt
     8341 gcaaatatat ttgtaagagt caacagtaaa ggtgtggtat taaagcaatc agattttgtt
     8401 ctaacacttc tttcagtctt ctgggaagaa ggtagaaaga aaatagaaga attctcaatg
     8461 caaagcacca attctaatct taaagactta aagttttcgt cctttaatca tctaatagat
     8521 gttaaagcag gagagatttt aagagcgata gtaggatatg gttttcatag aggacgaatg
     8581 agcgatgttt actcattatt aagagggaga gactttgaaa cgagagaata taacgaagag
     8641 ctaaagaaaa aaagatttgt tgacctagaa ggttttgttt ccaatggact agataataca
     8701 acttggcacg attatataaa tataatccaa tcattgggtt tcaagcataa agacttaatt
     8761 acatctagaa caaacttctt ttattcttac gctttctact taataggtaa atacaaatac
     8821 aatattgagt tccataaact tgatagaata gtatctagat ggttcttaat gtctgcctta
     8881 aaatcaagat attcaggttc ttcagaaagt gtctttgaaa gtgatttggc tctagttaga
     8941 aatattgaaa caggagatga gtttatttct cttcttaacg agagaataga ctcagaaata
     9001 acaaatgact tctgggaaat tacaatgcca actcttctaa cttcttcgta tgcaaagaat
     9061 cctcaatggt taatctttgt agcatcacag attagaaaca atgttgaact gttattctct
     9121 acaaagaaga tatctgacct ttttgaccct atcattacac ctaagaaggc tcgtcttgat
     9181 agacatcata ttttcccaaa gaattatcta aaaaatattg atgttgtatt gcaaacagag
     9241 caaaatcaaa ttgcaaacta tgtttatctt gattatatga ctaatataaa aatatctgat
     9301 gattctccaa caaactattt caagggtttc aaaaaataca gtaaagaaga tataaatatt
     9361 gcactaaaga atcacgcttt gcctaccaat ttctataaaa tgcaatacag tgattttctt
     9421 gttgaaagaa gaaaacttat ggctgaatat ataaggaaat attttgaaag tatctagata
     9481 tggacataac acaaaaagat acaaaagaga tttgtaatca atatttgaag gaagtctccc
     9541 taaaatattc tgatataaac acctctgagt atggatatag aacccccttc gaaaactttc
     9601 ttaaagaaat attcagtgaa ataaaagtta cgaatatcga ccacgatgga aaatctgttg
     9661 gaggtaataa gccagacttt gtcttatcag aaggaagtgc gcccctgctt tatatagagg
     9721 taaaagatat tggagtctca cttgataaaa ttgaaaaatc tgaacaacta gcaagatatt
     9781 tcggttatga caaccttgtt ttaacagact atctagagtt tagattctat agaaacggac
     9841 ttaaatatgt tgagcctatt tctattgcat catatgacaa aaatgaaaga actatctcat
     9901 ataaccaaga gaactacgag ttattaagaa aaacattaat acaatttaca cagtcccata
     9961 aagaacctat aaaatctggt tcacacttag ccaaaattat gggaggaaag gcataccgta
    10021 ttagggaaaa tgctagagag atgcttaata gtccaggtaa agaaaaaaga agtatttaca
    10081 aggtatatga gactatgaag atacaactcc ttcacgatat gtcttctgat gattttgctg
    10141 atatgtatgc acaaacactt gtatatggat tgtttgtagc tagattccac gacaaaacac
    10201 cagatacttt tactaggtca gaagctagag atttagttcc tgcttcaaat ccgttgctaa
    10261 gacatttctt tgaccatata gcaggagcag actttgatga tagattagac agtattgttg
    10321 atgagctctg tagagtgttt tctcattcag atatatcaaa gctcattagt aactattacg
    10381 gcacggagaa gggctctaaa gaccctgtga tacacttcta cgaggatttc ctacaagaat
    10441 atgatagtaa gaaaagaaag gaatttggag cctactatac cccgcttcct gttgttaaat
    10501 tcattgtaaa agctgttgac tatattctag aaaaagattt taagcttgta ggaggacttg
    10561 caaatagctc aaagaatgaa aaaggacata ttgttcaagt attagaccct gctacaggaa
    10621 caggaacatt cctaacagag gtaatcaaga taatacacga gaggagagag aaacaggctg
    10681 gtaattgggg aagctatgta ttacacgagg ttcttccgag attatttggt tttgaaatta
    10741 tgattgcacc atatacgatt gcccatttga agatgagtat gttgcttaaa gaaacgggct
    10801 tcaaatattt taataataga aggttaggaa tataccttac aaattcttta caggaaggta
    10861 acactattga aaatatgttt ggaggtttcg ggttagcaga cagtatatct caagaatcta
    10921 aagaagcttc agttataaaa aatgagaagc ctattatggt agttttgggg aatcctccat
    10981 attcaggagt ctcatcgaat gaaactcctt atgctaatag tcttgttaat aaatacaaga
    11041 aagaacctaa aggtgggaaa ttacaagaga gaaaacactg gcttaatgat gattatgtga
    11101 aatttatagc ttttgcagaa aacttaattg aaaaaaatga aactggagtc ttagccttta
    11161 taaataatca tagttttatt gataatccta catttagagg tatgagatat catttactga
    11221 aaacatttga tgatatttat atattagatt tacacggtaa ttccaaaaag aaggaaacct
    11281 cccctgatgg ttcaaaggat gagaatgtat ttgatataca acagggtgtt tcaattaatt
    11341 tatttgttaa aactgggaaa aagaaacctg aggaacttgg tagtatttgg catagtgata
    11401 tttatggaga tagaataacg aagtttaata tattagaaaa aagccaacta aaagatatta
    11461 actttgaaaa agtttcaaat agagaaccat tttatttctt caaaccaatt aatcaagaag
    11521 ggcgagagga atacgataga ggatttagtg tgaaagactt gtttaataaa tatacaacag
    11581 gtattgtcac tgcaatagat agactttcta tcttccaatc ggagaaggaa ttacgagaaa
    11641 aaacaaacga aattcttaat tcgagtaatc cgttccaaga gtttgatatt aaagaccaga
    11701 gaaaacatac caaagaagaa cgaattgaag aattacggaa agcatcagaa aatttaccta
    11761 caaaaatctt atatagacct tttgatatta gatatatgtt ttacacaaag acttctgagt
    11821 gctggattaa ctcgcctaga tatgaaatta tgcagcattt aattggtggg gataatttag
    11881 cattaataat accaaaacaa acgaaaggga agataggagc ttcaattact cgatacattt
    11941 ctggacataa aacctttagt tcatacgata taaataccct attcccatta tatctctatg
    12001 atgtagaagg taaaaagaca ttaaatttga ataaaagtat acttaatgag tttgaggatt
    12061 ctattggtca actagagcca gaaaagttat tagattatgt atatgccatt cttcatcacc
    12121 cttcatttag ggaaaaatat cgtaaattta ttcgtgttga ttatcccaaa atcccttatc
    12181 caaaagatag gaagactttt gataaacttg caaaattagg agaacaactt gttaaatatc
    12241 acctactaga acacgagaat attgacccat atcaaatatc atatatagga gaaggagaga
    12301 acattgttga aaagcctgaa tataaggatg ggaaaatata cattaaccct actcaatact
    12361 ttgggaatgt atctgagata gcttgggact tctatattgg aggatatcaa cctgctcaga
    12421 aatggctaat agacaggaag ggaaaagaac tgacaccaga cgatatcgaa cattattcaa
    12481 aaatagttct agtcctaaaa gaaactgaca gacttatgaa agaaattgat aaggtaaatt
    12541 ggtcaaaaga taatgaaaaa taatgttaaa actaaaaaac gaactacccc tcagttctcc
    12601 aaagtggtaa atacatcttt aacttttgac ttaacctcat ataaaagggt tgttactcag
    12661 attgattcaa ttcttgaatc acaaaagaat ctttttagca gcatagataa taaactagat
    12721 gtatataaaa acatcttaga ttctgaatta tttaagcctt ccaattcttt gtgtaaggtg
    12781 ttagaagagg ctacaaagcc ctctaaagcc ttgcaaatgt tagttgagag tatgaactac
    12841 ccttctaacg ctcttgtgaa aactattcaa gaaatgacta aacctcagaa gctattggta
    12901 aactctatag aggaatctct taagagtatc actgaaccgt tctcaaaaat agattttaca
    12961 tcataccttt ctagtatttc agaaattgaa ttaagtcttg caacaaacct agttgtcaag
    13021 acagaacttt tgaaaccttt tgaagggata tcaaatggtg tttctacaaa taccgccatt
    13081 attgataaac gactaataaa caatggtgaa attggcttaa gagtccagaa agatacagca
    13141 acaatatcaa cacaaattca ataccagaaa tttgagatga ttgatacaaa attatctatt
    13201 attcaaacga aagttgataa cttcgagcag gatataacaa aagagcttaa agaaaggttt
    13261 gttactttag atagaataat tgaggaagtt gaaaacgacc cttttaagtt ctttaaggtt
    13321 aaatcattcg aatttataaa ggaaggaagc acttttgtta ttaataatgt aagaaagata
    13381 tcattagtat caggaacaat tcaggattat atttgtcaga tattatttag tggaataaaa
    13441 gaacttgggg aagaatggta ttgggaagat atttttgatg agatggtagc aaaatatcgt
    13501 tatgaaggga aatgtgaaat gacttggaag tctatgcaag atgcaattgg acaattaaac
    13561 attaaaatcg caacggaaac cacaattaag gatgcatttt taactcctag gtctgagatt
    13621 attcaactca acccattata tttttcgtcc gacctcgtcc gaaagcaata gtctaatata
    13681 ttcttgttta agatgaacaa gaataaaaat gattatagaa agcaaacttt ttcaaactca
    13741 agacttaaat cttactgcgg tattgatagt tgaatcattc aggcttgatg atattgaaaa
    13801 gaacaaagaa ggtaaagcaa cctttatctt ccgtaatagt ctatcacttc aaacaaaaat
    13861 ttctcagtat tggaataacg agttacttgt aaatccacaa gacttattta atgcactaaa
    13921 gctccttaaa aatcgaattt atagcaatta ctaacaatgg tgagcgaaga aggattaata
    13981 aaattcaaaa agatatatga ggagaattat gaaattaaac tctctcgtaa ggacttaata
    14041 gcaagagcaa ataagctatt gaacctttat aaggcagttt atggtcaaag aacaaatata
    14101 aaaatagata ttaagaatga aaaagaaata cagcttgaga aaactgaata aacacaagag
    14161 ttatgattca tctgagcttc aagaaatact tggagttcat cctcaaacag tcaagacttg
    14221 gaagaaggaa ggattaaacc caataaatgg aggtagttca ccctttctat attcgggtga
    14281 agagttaatc acattcctat tgaaaagaac tgaaagtcaa aaagttaagt taaaggaaga
    14341 tgaaatttac tgcttaaaat gtcgaagagc tgttaaacca attgacacag aagttgttag
    14401 aagaggtgtt atcttgggaa gtggtaatga atctacaatt ctaaaatcta attgcccaat
    14461 ttgtaaaagt aaaattcaga gatttagctc tataaaaaga gaagttgatt gtgaggtaga
    14521 aacgaacgag aagaatcaac tcgacctatt tcggtgagta gttaagacgg acattattat
    14581 agtcttatga ttccccttat atcactacct taggaagtag ttaaatttaa taaaaatagt
    14641 atgggtaaca aactgtatca aaatgagaag atgaagagga aatattttga tttttgtagg
    14701 aatgcaaaag gtttttctat aaagacaatg aaaaagttag aaggcgctgt ttataaatac
    14761 gatgagtatt caaaaaacat agactacaag caatttaata gtgaaatagc taaaggattc
    14821 aaaaaatggt tagcaagtca taagaataac aaaaaacaat tgcttgctct ctcaactcaa
    14881 tttcatatcc ttagacacct gaaagggttc cttacttggt tatcacaaca atcgggatat
    14941 aaatcaaaaa tatcatttga tgatattgaa tatctttctc tatctagggc tgagtcacgg
    15001 atggctactg cctcaaaaca gccctcctac ccttctcgag aatatatact gaagattaca
    15061 agttttaaga taaacaatga tatagattta agagatagag cattgatttc atttacagca
    15121 ttatcaggga tgagagattt ggctattatt acattgccat ttggagctta tgataaagaa
    15181 aatcaaatag tcaatcaaga ccctagctta ggagttaaga caaaattctc aaaaactata
    15241 tctacgaaat taataatttt tgatgcgaaa ttacttgagt attttcattt gtgggttgag
    15301 agattgaaga ataaatatat ttttagtatt aacgaccctt tatttccagc tactgaggtt
    15361 ggtttgaaaa gtgatgttga acagtctttt atcgctattg gtattagcaa gtcctcttgg
    15421 aatgatgctg gtgcaatgag agagattttt aagaagagag ctatgcagat ggatttggat
    15481 tatttttctc ctcataagtt tagacatttt aatacaagtg aagcattaag atatgcaaca
    15541 aatgcagaac agataaaagc aattagccaa aaccttggac acgaaagagt ttctacaaca
    15601 ttgtttagtt atggtgcaat tgataatttt agattgccaa aggtgattga ggaaataaat
    15661 gaaggagtca aaaatggtaa taacctttaa taggttaata taatgatata tgatttaata
    15721 agataaagat gaaaagccta ttagaagaaa acaattattc aaagaaccga tacattctct
    15781 atgctcgtaa atctacagag tcagaggata gacaagtagc ttctattgaa agtcagataa
    15841 aggttatgaa tgatgttaag aatcagctga accttaatat tgtagatgta atacaagagt
    15901 ctagctcagg ttttaagata ggaagaaaag aatttagtag aatgcttcat atgatagaga
    15961 atggagaagc tgatagcatt cttgtttgga agctttctag attgtctaga aatcctgatg
    16021 atgcagggag aataatggga atgcttcaaa gaggagaact aaaacatatt agaactttcg
    16081 atagagactg gtttccaact gataatgtga tgatgatgta tgttgagttt ggaacgacca
    16141 atcaatttag taaagatttg agtgatgata caataagagg gctgaaccaa aaggctcaaa
    16201 gaggttggtc tccaaaatcg aatctccctc ttggctatat gcataatcca tacaaaaaaa
    16261 cagacagtgt tgagatattc ccagaccctg agagatttga gataatccaa aaagctatga
    16321 agaaagttgc ttcaggagag atgtctccaa gagaagcttt gaattatgct aatgatttag
    16381 gacttaaaac aaaagaaaca agaagattca aaagtaaagc tgtatctaat tccgtatttt
    16441 acagaatgct ttcaagcccg ttttattatg gattgtttga atatccaaaa ggaagtgaca
    16501 attggattac aggaaaacat aagaaaatga tttctgagga ggaatacaac cttattcaaa
    16561 gatataaggg taaaaagaat gctccacgac ctcagaagca cttattccca tacacaggac
    16621 taatgaggtg tggtgaatgt ggatgttcga tagttgtaga cccaaaagaa aagattcaga
    16681 agaatggtaa taagcatagt tatcattatt acagatgcac aaaagctaaa gggaactgtt
    16741 ctcaaaaata cttagaaaga aaggaattag agaaacaatt agctaatatt cttggttcaa
    16801 tagagatttc taaagatttt cataattggg cattagaaga attgagtaag gatattgaaa
    16861 aggatgtttc tgatagggat gaaataatta aggttgcaca ggagggttac aatgattctg
    16921 taagtcaaat taatacactt gttaaagcat atgctagtgg gaaagttccc gagagtgctt
    16981 acttaacaac tataagagaa cttcaaggcg aacaagacaa gttcaaatct atgattgata
    17041 ctacagatga aagaataaaa gaatttgttt cgaaaacaga agaaaatatc aattttgcaa
    17101 aaacggctca aaaggaattt gaaattggtg atatacaaaa gaagaacgct atcctaaacc
    17161 atttaggagc taatattgta ataaaagatt tgaaggtaag tattgatatc caagagcctc
    17221 tcctaattat taagaaaggt gagcagaagt ttgtcaatat tagtaaaggc ttagaacctc
    17281 ttgaaagact gtcaggaagg gataaagctt acatttttga gtcagatagt gtcatatggg
    17341 gtggaagatg ggatttgaac ccacaaccct cggttccaca aaccgatgct ctaaccaatt
    17401 gagctacatc caccataacg ataccaatta tactacagtt atacctttac aggaaagaaa
    17461 tctccctttc tcttctcaca cctatcccct acattccatg aattgtaata acccatgtct
    17521 cttgttgtaa acctcttctt cttaccctca tctgttttaa aaacaatata gtaggaatct
    17581 gaactatcct catctccaga gacataattc ttcttttcaa atgtaccctt ccatatctct
    17641 cccttcttct tactaaccca ccaaaccata attacaacta ttacaactac tagaataata
    17701 ctgtatacat actctggcat acttaagaaa ttttaattta tttagtaact aacaaaacta
    17761 cactcacaag ccctactctt tacctctata ccctctttta aaccattgga tattatctct
    17821 tgacccttgt catctagata aaggaaagga cactcatacc tactgtccat gctattatct
    17881 atcccaaatt caaacatatt accatctaca taccccaata tatctcctga agtcatatag
    17941 tcccctacag acactctaat atttgtaagt gaagagaaat atatcaaaac attatctcta
    18001 ctcctaagat acacgacatc ttcctctatg ctatctatgg ttccgtccac tggtgccata
    18061 acctgagctc tctcctttgt ctctattact atcccatgac tcttcttctc acaaaaaggt
    18121 gttatcccct tctcaatata tctcatatct aatctctcga caggaagcgc catcttcaaa
    18181 ttaagaatat tgctactagc atctaacttt ggaagaaagt agaaggtaaa tattaaggca
    18241 ccaacgatta ccaccccaaa cagtatgaac agtatagttg tatttctcat actctccaat
    18301 tattaattta tttcatcaca ctcggtgcaa gtccttcgaa gtttaatcca gtttctgtta
    18361 tttctaaggt atctattctt aatgtatctc ccatattagc aagcttttca ttggcaagct
    18421 caagcagtgc actctgcaca acaggaacta ggtataccgg aagtgataca cctgctacct
    18481 taagagaatc tagttgccca gttatgctgt ttgaagatgc tttctcacct gttccagaga
    18541 catacacagg atacaccttt ccctcatact caaacagtgc agaaatctct gctttgccat
    18601 cactaaattt gatctgtaaa tcgtctaact ctccatatgg aagatcgtga tttgcatagt
    18661 ttaaccaagc agtcgcctgt gagcttgtaa acgtaacctc tacctcatgg ggtttgctat
    18721 atattggggt tgtacaatct aaacacatgt ctccagtaaa gccagcattt gcaaaacttt
    18781 cttctaaatc tgccattgaa tatgtcacac ctaaatctga gggagtattt aatcccttca
    18841 ttattttctt gtatccaaaa tatgcgccac caattaaaag gccaaacagg agaaatacaa
    18901 tcaagaaggc aattaaacag ccattggctt ttttctttgg cttactatcc tctattggag
    18961 gatttttaac cgtagtatct tccatatttt atgggtaaaa ttttattaga tatattgtat
    19021 atcaaatgat tgcacagtta cagatttttc tatttcaatg ctccaaacag taaatcttgt
    19081 atatctacat gatcctttgt aaacctaata ctctcaaact caaacgaaga taaaccttcc
    19141 aaaattactc ctgctatcca ctcctcttct gttttagcta tcttctcctg tacccagtct
    19201 ggtaatctca aaagtccaat ctttatctct tgtatatcta attcaaaatt ctctctagta
    19261 aattcttctg aattagcaga gacatacagt ggaagattaa aaggttcccc ttcggaaaat
    19321 tcaaactttg ctaaagtaga aatctggaca ttatctttat tgaatttaat ctgtgtattt
    19381 gaaaatgtag gacttacgga ttgagcattt aacatagcag ttatctgtgc actagaaaaa
    19441 ctaatatcta caccatcctt tatgtccatg tagttctccg gtgttatctt tacaatctct
    19501 gttaaatcta ttacatcttc atctgtatat cttacgttta aatctacctg agtctttaaa
    19561 tcagaactaa tctttctgta tccgatatat gctccaacta ttaacagcaa aaacattcca
    19621 atgaacacta ggaaggtaac taagcaacca ctcaccttct tcttaggttt aatctctaca
    19681 gcagtttcta ctggttgagt aatttctaat tcactttgta atgtgtcttc catatatatt
    19741 ggataaggtt attcaatatt gtatatcaaa ttattttaca gttacagatt ttgctaaatt
    19801 ccttggttta tctatgttat tacccaactc caaagagagg aaatatgata caagttggaa
    19861 tgcaattaca tttgataatc cagagagacc attcacctga ggtgtaggta tgaagaagtc
    19921 aaaaagagta ttactaaatt tcttgtcccc tacccctatg gttagagctc ctcttgcttt
    19981 cacctgttcc atacttgaga tcatatcttt actgtcttga tcatctgaga ttatcccaaa
    20041 tacaggagtt cccttctcta caagagcaat taccccatgc ttaagttctc cagccataaa
    20101 tccctctatg tgtttgtagg agatctcttt aatctttaat gcaccctcta gagatatatt
    20161 tgcattgctt cctttaccaa gtacaaacag atgttcacta ttcttaatct tcttaacgat
    20221 tttcttaaca ttcttgtagg tatctatatg gagaaacttg cctaatttta ctgaaagatt
    20281 tgcaacatcc ttttgtgctt ggtcatattc attagcaatt gtcttagcaa ggagatatcc
    20341 ccaactacac tgtgcagtaa agacctttgt agaggctaca catatctctg gaccagcgtt
    20401 ggtaaaatac tggtgattgg acattctaga aattgtggtc cctagcatat ttacaatact
    20461 ggctattttg catcttttct tttttgctat ctctagtacc tctatggtat ctgcagtttc
    20521 tccactttgg gatactgcaa ttactaagtc tttacttgag aggatattta tatatgattt
    20581 gaattcatag ctttttacat cctgtgcatc tataccagag atattcctta gaaaatatgc
    20641 tatctgtcct gcagcaaaac cagcagtacc tgcccctact acatacactc tgtgagcctt
    20701 ctgaatatcc tctacgaggg gttttagctc tttcttggta tattgtgtag cgctgatgat
    20761 tgtattttgt tgttcaacaa tctctttgag catgtaatgg gcatatttgc ctttgttgat
    20821 gtcgacatat ttgtttttta gtacatgtat ttttacattc ttcttcttag aaaggatatt
    20881 cgttatttcg atcttcccgt tggatatatg cacaaaatcg tgatcatcaa gaagaacaac
    20941 tttgttagta tgtggagaga aagaaagtgc atctgaagct agatatatcc catccttgga
    21001 tttcccaatg acaagtggag aaccatttct tatggcatat atatcgttgt ctttggtcag
    21061 gattacgatg gtgttacgac ctttgagcaa cttgaaggct ttggttatac aagttttaaa
    21121 aggctttttt gtcttctgtt catcctctat gagttttacg attacctcag tatctgtttg
    21181 tgttttaaaa acatatccct tttgttctaa ggattttttt agttcagaat agttttcgac
    21241 tattccattt tgaacgagag tgaagaattt gttctcactg ttatgtggat gtgcattgtt
    21301 tgtagtcaca cctccatgtg tagcccatct tgtatgtcct atggctgtat ttacagatgg
    21361 taattttttc aattcctgcc tttctgttat ctctccaaca gttttaaaca cctgtatgtg
    21421 ttgcttgtca ttaacagcaa tgccccaaga atcatatcct cggtattcta atctttttaa
    21481 tccttgaaat actaaatcat ttgcaatttg tttgccggtg tatccaaata ccccacacat
    21541 aagcgtagtg ttaacaattt atatcaatgt gggtattaat gactaatctt ttgtttattt
    21601 gagattaatc ttaaggctca taacggaacc taggagtacc cgctgattgt tcgaattact
    21661 cctgcctcgt agcaatgtat gcccagccgc ttttaaatta tgtacccata tatatgttac
    21721 agaaatatat catatggtca agagagaaat tctttgatac gatttggtat gttaaaggaa
    21781 aagattctaa cagagttaaa gagtgtttct aaaaaggaga aagaggaaat attccaaaga
    21841 tttttcaaaa caggtaaggg agagtacggg gagggagata tttttgcagg cataactgtt
    21901 ccagagattc gaagtatttc taagagatat tacaaggaga tgactttaga agaggttgag
    21961 tattttgttc aacacaaaat ccatgagtac agattgtttg gattactaac acttacatat
    22021 atgtggaaga aggcagatga ggtaaaaaag aaaaccgtct ttaatttgta tattaagaac
    22081 ttaaaatata ttaacaattg ggatttagta gatctttctg caccaagtat tgttggagag
    22141 tatttaaaag agaaagacag aacaattctt tatgacttag caaagagtaa tgatttgtgg
    22201 aaacagagag tagctatact ttctacattt tcatttcttc gagagaaaga tttcgtagat
    22261 acactacata tctcggagat attgcttcac cacaagcacg atttaattca caaggcagta
    22321 ggttggatgc taagagaaat tggaaaaaga gatcaagatg ttgaggaaga attcctgaag
    22381 aaatattaca aagagatgcc tagaactatg ttagggtatg ccattgaaag atttgaggaa
    22441 gagaaaagac agaaatatct taaaggtttg gtttaaattt cctaagaagc aaagtaacaa
    22501 aagttgtccc aaggacggca gttatgaact gtatcattcc cattgcttgc aagaatgctc
    22561 ctggcatatt tactaacctc atcaagagaa gaaccgcact gtaaagaatt aatgctttaa
    22621 tacttgcaga gattagtatg tttacatatg gaagtttaat tttgctgtag agatatacta
    22681 aagcgaaatt agacagtgca atgaaaggaa ttaagtaaag tagaaaaatt gtagaggaac
    22741 caaagagtaa tccttgtgtg taggaaagaa gtgaaggaat aaggagcagt ggaagtgttt
    22801 ttcttaatcc aaagcgtgtg acagagaaga taaagagtgc atttattaat gtgccaagaa
    22861 gtatctgagg tttaggtatg agaaggggaa gaagaattgc taaggagtat gtaatgagta
    22921 actgaatctt ttctgattta agtatttcta attgtttatt taagagaatc atacttcagt
    22981 ctatttcaat gcatttatga actcaagccc tatagcttat taactttcca tatgcaattc
    23041 tctttgtagt atatttaaat atggaatttc aatctggttc taaatattta tgtttacttg
    23101 cgccatcttt tgtctcagag tttgaatatc ccgaggtaat atacagatta aggatgttgg
    23161 gttttgataa ggtcttggaa ttaacgtttg gagctaaaat ggttaaccat gcatactacg
    23221 aaatactaaa ggaaagtctt gagaagggag agaagaaaac ttggatatct acaccatgcc
    23281 ctactctagt aaatttcatt cgtgttaaat atcctgaatt agttgataat cttgttccgg
    23341 ttctttctcc catgggagct atggctttga tatgtgacaa gttctatcct ggttacaaga
    23401 aggttttcat tggtccctgt gtcactaaga agcttgaagc tggagagatt ggtactgtag
    23461 atgaggtact tactttcaag gaattggaat tattgtttaa agaaaaaaac attccagaga
    23521 aggttacgga ctctaaatat tgtgtgactt ttgacaaatt ttacaatgat tacacaaaga
    23581 tatatccaat atctgggggg ctatcctcta ctcttaatta cagcgatata ttaaaaaaga
    23641 aagacatctt ggtaatggag ggtttaaacg acataatgaa gatactggga ggatttaagg
    23701 atggtatgta caagaattac aaatttttag atcttttgaa ttgtaaggga ggatgtatta
    23761 atggtccagg tatgattgga gataggagtg tgaaggaaag aagaaagaga gtaatacagt
    23821 acagggatta tgcacaaaga tatgagaaag atttaggaaa agcaggaatt aaggtagatg
    23881 cagagggtat agagtttggg agaaaatttt agctattttc ttactccttg atgtacagtt
    23941 taatatctac tacaacagct cttttctctg ttaacataac aggattaata tccatagata
    24001 ctatctgagg atacgatatg agcatctttt gtaacttcat cattaaatct aacaactttt
    24061 ctcttgctaa ttttggttta cccctgtatc catctataat agttgagact tttgtttcgt
    24121 ttagaatact gtccatattt tctaaactct ctggaagtaa tatgtgctta atatctttgt
    24181 aaacttctgt atatatacca ccttgaccta ttgctaataa atgaccaaaa cctaatccat
    24241 ctttctcata aatatttgaa ttaccctctc tgtttgtacc aatgaagaac tctaattttc
    24301 catctatcat ttcctgcaca aggatacttg gagattgttt ccctgttact ttctcaattg
    24361 tggtttttaa ttcttcaaat ttctcttcga attcagttat tgttcggata tccaggtaga
    24421 tggctttaaa gtcagtcttg tgttcaaatt cctctgcagt tgcttttata gctacaggga
    24481 aattaattgc agcaaaatta attgcctctt caatgcttcc tgttacaagc tcatttggga
    24541 tatctatctt gaattctctc attatggaga tggcaagttc atctggaact attacagcag
    24601 tattttcttt tagtaatttc ttaatttgtt ttgtatattt acctctcttt ttaaggctat
    24661 ttgcttcaac aattttttta ttatcttttt cctcttcgaa tacagagagc tttttgatga
    24721 ggtgtacagc ttcttgtatg tcattgaagt aaggggcatt gtttagttct aagattctaa
    24781 atccagcctt aatatctttg ccacccaaga aacaactgta tattggtttt gattctgtct
    24841 tagatatctc tacaattttc tgtgcagtac cttcgatttc tgttattagc tgtggagtta
    24901 ggataacaag tatggagtcc gcatcttttt cttcgagaac agttttaatt gctaattcat
    24961 atctgtcact cttagcatct ccaaggatgt cgattgggtt tttaatactt gattctcttg
    25021 gaagagcttt aagaagattc tgtttggtct tctcccctat ctctgttagc tctagtcctt
    25081 ctttttctac catatctgtt gctatgattg cagggcctcc tgcatttgta atgattgcaa
    25141 ttttgtttcc cttaggaatt gcattaatgt ttatgaggga tattaatttg aaaagttctt
    25201 cggaattctc tgcaagaata atgtttccct ttcttactgc tgctatggtt gtgtcatatg
    25261 atgaagcgag tgcacctgta tgggaagata ttgctagttt agcttcttca gaacttcctg
    25321 gagctattag gagaattggt ttcttagcct tttgtgaaag agatacaaac tcttttccat
    25381 ctttgaattc ttcaaggtaa agagctaagg ctttgacatt actatctgca aaaagttgtg
    25441 ccattaattc attctcatgg atgtctgctt tgtttccaag agatataaca tgggagaatc
    25501 ctaatccttt ctcacatgcc atatctaaaa ttgctgtaca gaatgctcca gattgagaga
    25561 taaaggcaat atttccttct cctggatttt ctcttgcaaa tgagaggttg agttttgctc
    25621 tgttatttat aaaaccaaga caatttggac caagcaatct aattcctgca ttttttaatt
    25681 tctgtgtaat actttcttct agtttttttc cttcttcgcc tgtttcctta aatccagcag
    25741 agattattac aattgttttt acttttttct caatacactg gtcaacaatt tcatctacga
    25801 attgataagg tattgctaca cagacaaggt caatggaatc tggaacttca agaattgtag
    25861 gatacgcttt tcttccttgt atttcttcgt atttaggatt aatgggatat attttaccgt
    25921 tgtatccccc ttcgatgaca ttacgaagta taacggaagc aattttctct gagtctgtgg
    25981 atgcacctac aattgctact gattttggat ctaatgttga atttcccatt ttctatattt
    26041 taatattata ttctctgact tcatctacta gctctgtttt tctttctttt ctgtttggta
    26101 gagaatcgta gtaagttggt aatttactgt ttttgtatat tactcctgtt gcaatgtttt
    26161 ggtcagttat ctctactaat tctctcgctt tttgtatgtc gctgactgtt tctaccttct
    26221 ttactctttc attgtaccac tgtggtgaag attgtgggtt gtatgtaggg catatttgta
    26281 atatttcaat tactgaacat cccttatgtt tgactccttc tttaattatc tctgttaact
    26341 gttgtggatc tgcggagtaa cctcttgcaa agaaagaggc ttctaggctt aagcatagct
    26401 gaccaatgtt aatactgttt tctggtttac catttggaaa tccaaagaca gatttatctt
    26461 tttgtgtcgt tgcagttgct tgaccggttg taagggcaaa attttggtta ttgtgtatga
    26521 ggatggtgat gtcgtagttg gatctaattg catgtagtaa gtgatctaga ccttcatgta
    26581 gacatcctcc atcaccaata tctgcaatga cggtatactt tctgtttgct agatgtgctc
    26641 ctacggcaag tggaattgat ctgccatgta atcctttgaa tgcatatgta ttaattacat
    26701 ctgcgccatt accgttacat cctatgtcaa aggatatgaa ggtttgagat ggcttaatat
    26761 ttaaactttc aagtgccatt ttaagggatg cgtgtatggt aaagtttcca catcctggac
    26821 accatgttgg taatttttca ctgtcaaaac tatttttcat tctttaaata ttctaatata
    26881 tcttctacaa aaaatggtct agcatcgtat ttaagaagtc ttttcttaaa tgagtatcct
    26941 gttttctcct ttaacatttc cgctaattgt cctgtttggt tgttttcaat tacgattatt
    27001 ttctttcctc gcttaattaa tgtgtttagt aattctgttt taagaggaaa aagatactca
    27061 taatgcagat aaccaatatc ttctccttgt gagattaaat ctaggactgt attttttaca
    27121 cttccccacc ctacgagtat agttttggat ttgtgaggat tgaaaagtaa gggttgtggg
    27181 aattcttttt ctaatgattt cacctttctc attcttttct ctgtcatctg tttaatctct
    27241 ttactgtttt ctatgctttc tcctttttca ttatgttcat cacttgaaga gaggtatggc
    27301 tgttcgtcaa aggttgggat tcttcttgga gatattcccg agtcggttat gttgtatcta
    27361 aaggtcatat ttgaaagata tcttcttact gggagattgt ttggtagttt gtttacagta
    27421 aagagagatt ctgtaatttg tttctctgtt agtaaaatta ctggtatttg gtatttttct
    27481 gctagattga atgctttctg tatgagggta tatgatgatt caatgtcact gacggcaaga
    27541 accgcttttg gatactctcc gtgtccagct ttaactgcaa tgtttagatc gctactacca
    27601 ctccatgttg gaactcctgt acttgctcct gctctctgac cgaggataac tacaaggggt
    27661 gtttctgtca taccgctaca tgatatggtt tctgtcatta ggtcaaatcc tccaccagag
    27721 gtggctacta gtgctcttgt acccatatgc atgcttccta gggccattag aatggctgta
    27781 atttcgtttt ctgcttgttt cagaagcaca cctttttctg ttccatactc ttgcatatgt
    27841 ttaaatatgg cggtaattgg agtcattggg tatgcatagt aggctcttac tccacaggat
    27901 atagctccaa gagcaagcgc ttcattcccg ttgagtattt tcttggatgt aattctaaat
    27961 ggaacggtag gtagttctat ctttttaaca tattcaatgc tgtatccttt ttcgatacat
    28021 tttttctcga ggtcagaatc gacttttttt tctttgaaat attcaagtat gtgtttttcg
    28081 acgatatttt ttttaatatt taaaatctta catatgaagg caatcatgat tgtgttttcc
    28141 attatttctg gggctttaag ctcggaaatt atctgtctgg aatctatgaa gatttctttt
    28201 gtataatctt ttaagttatg aataacaatg ccgttttttt gtaatgtagg aaggtattct
    28261 tctagagcat ttttatctag tgaacagagg atattacagt atcttgagga gcagtttatc
    28321 tgtttatctg agaaatctat ctggtatgat gcaacacctc ctttgattac agatggatat
    28381 tctctgtaag agaatgttat gtagcccact gaattaagta ttttagaaat gattgctcca
    28441 acagtattaa tcccctgtcc tgactgtcct gctactttta cggaatatct tgtgagagac
    28501 atagtttgta ggtattttaa agtgatcctt gaccgttgaa taatgagtag tcaaatacag
    28561 gactactcca ttttctttct ggtacgagta tctgtttgcc tctgtatgta ccatcacagt
    28621 tggcagatgc ttgtacatcc actattctaa agccttgtgt acattctgta gtaagagcgt
    28681 atcctacccc aagtgttcca ccgattgctt ttactctaat ttcgtccagt gcatatgtcc
    28741 cagagagctc attaaggttg aatcccaaag ttcctccgtc agctattggt atccactcat
    28801 cacctaagaa cttggtctcg ttacaggaga aacagtactg ttttacatcg tctatggcgt
    28861 atggtttgta cttttgtgca atgccgttag tgtagttttt cccatatatc ttttcgactg
    28921 aaaatcctac ttgagaatct cctcttgtcg caaatgacac tgatgctcta catcctgctc
    28981 caactggaac tccttttatg gggaaagaca ttacctgtcc tgcccgtatc tcaaaatatg
    29041 tatctggtga tgcttcttta attttaagat agtattcatt tccacctgtt cttatgggac
    29101 agaaattaag agtagaaaat atctcttcta ttcctaaagc agtaaagagg tcagatattt
    29161 gtccgttcga ttcggtcaat gttacaccgt tttcgtaatc aaaagtattt ccttctccag
    29221 ctatagtttg tgtaattgta tttatgggga ctgtggtgag ttggttaagg attaaatcag
    29281 aaacttcaag ggcttcagca gatgctcttt caccaacaga gagtaatttg tcttttgcag
    29341 ctctagagta aattgccaac ccaatgattg aagagactac taaaacaacc attgctatgg
    29401 ctagggcttg tgcattgtat ctgttgtcta tatttttcat atttagaatg atttaaaacc
    29461 tatttcttgc ttatatattg gggcctctac tccaaagaac tgtgtcgcaa tgtttggata
    29521 tttgtagtcg tagtttatga caaccgttgg gttagagatg ttggaaagtt tgaggttacg
    29581 aagaacattt atagcagaac cacctgttac tccattacca aatgctacca tgcctccatt
    29641 tatctctatt ccatctctca cagatttgtc tgtatctaca agattaaata tgatttgtcc
    29701 ctctgccatg agatatgcat ctaggtagtc gtagtgaaca ttagtattta ctcctgtctt
    29761 gtatgttcct gcacctatgt atatatttcc ttttgcaagg aatatacagc ctgtgtttgt
    29821 agaactactt gtaatgtcag ggtttatggt tatgtttcct tcactcatta caagaatctt
    29881 acttgtacaa ttaaatcctg tgtttagagt tatattattt gtactatata catagcagat
    29941 acctgaagca catgaagagg ttggtgttgc tattttggtt gtaccaggta ccttttctgt
    30001 ggctaatttt tctttaaaat gatcaaacca gtaggttttt cggtcatttg aatcatagac
    30061 atttagtgct cttactgctt ttaattctgg atgtagtaat tctggtaata tatcatttct
    30121 tgcagagatc aattctgtac cagtatctaa ttctgcacta gtaagtttta caaatacacc
    30181 atctaaggca ggaagtcctg cgacatcttt tgcagcattg gttatgccag aggatgaata
    30241 aacaaatcca ccttttgagg ttatccaagg atttagattt atatctttat attgtgcatt
    30301 tgtattacat cccatatcgt atgctgttac gtatgtgctt atcataccac cttcattttc
    30361 accaacattt attaaatcta cctgtgaact tgtgtttgta aatctccatg cgtttgtgtc
    30421 gtcaaacatg ccgatgagcg tttcttctgg tatttgtgta agagttatag ttcctttgct
    30481 tgtcgtgtaa tctggaggta agaacatttc taagtcgttt gttatggatc ctcctgttct
    30541 gtatccattt ataaccgttc tcataatgct actttcatta tcatttgcgc tccaatctag
    30601 gtaaacgctg ttgacatctt ttacggtttg ggttatgtca ttaacagttg ggttagcaag
    30661 gtcaattccc caattaaata ggtcttgtat tctgctttgg tctactatgt tattgttaat
    30721 catgtaggaa tctacagcag taccgtatac attgtatagt ccagatggat tgtttgcagt
    30781 tggtttaaat tctaaaccaa atgatatatc taaagatgcc ccttgtgtga tagttagacc
    30841 ttgtacagcc aatttgtccg ctgcagttag gagtctccat gtgttatccg tatttgtgga
    30901 gtaaattagt ggggtattcc aacttcctgc atttttgcgg atcatgatac caaaatcatt
    30961 tgtattagat tgtgtgtatg ttcctgttat gtttactaat ggtggaacag tttcatcttt
    31021 actaaaccat attattaagg cttctatttc atcgtttgag tctgcatctg taacagatgc
    31081 attaaatttt aatgggttat taatatctag gcctgtgtat gtagttgatg tacagttttt
    31141 ggcggtttta tcaattgtta tatcaattgt aatattggag tcagggatgt tatctacttc
    31201 gtagtatccc tcaataggta gagaatattg tttggttgta ttacatttat taattgtgta
    31261 atacattcca gctatctttc catctgcatt ttcttttagt acttggagta ttgttttggt
    31321 tgttgggtcg aagtcttgta tgtttactgt atctttaatg aagaagtctt ctccagcgac
    31381 accagcgcat gatgtgggag tacatgttgt tatggcagcc cattcatcgt ttacaccatc
    31441 gttaaatcct ttaaaggcat agcctcctcc attagatgta gatgggaatg ttgttgtggg
    31501 ttcttttact tgtacattag atgcagtaga tggtagtttt attcttgtag gagtgtttga
    31561 attagtagag aggttgtatg ttacattgtc tactacaatt ttaacagagg ttggcataga
    31621 tggggctaca ttggtttcta aaagatagca gtttcttgtt cgagatccac aatcagttcc
    31681 acaatcctca cctcctatat cacaagatgg ttttggtgta tatacagttg tacagctagt
    31741 taatgatgta ttgtctgtgt agcctcgtgg gcatgctgga agagtacaag tagagtcaca
    31801 atcacggaca caacactcaa cggatgaacc gtttgggtat gtaccatcaa ttgagctgca
    31861 agaggttttt gtttcgggaa tagatgtctc tgtacaagtt ctacaggccc tagtacagtg
    31921 catataacag gttccatctg ggcatggaca agatattgtt ccaattgaac accaagtccc
    31981 tccacacact agctctgctg atactttgtt aatttgcaat aatccaataa ttgcaactaa
    32041 aaatagaata gaaagatatc gctttatcat tttttataaa acaaatttaa attaatacat
    32101 ctattcatta ttttgaaaag cggatcgcta ttctctagaa agaatttaag atataccccc
    32161 tctttttcta gattattttc aataagaagt ttttcgcatt caccagttct tgtctttgtg
    32221 atattagata aaagggtttc ctttatatat gttttgcttg attcattctc tatttcacta
    32281 tatattttat acatattgca aaagacactc tcactaatat tcgatcttgt aatcgcatta
    32341 tttagatttt ctaaaacatt ttgctttgac aattggacgt atttttcaat caagtattca
    32401 taatcataaa gaaaagagat ttcctttaag agtgaaagag tgtctgtttc tgactgtttt
    32461 ttttcaaaat ctattgttat aaagggaatt acgttattaa tactaataga gctctggttt
    32521 ggaattgtat ttgtattaca gacatcagtg tatccaacat tccttgcaga atcacaggca
    32581 aataataacc ttattgtagt atcgtctaag ctatagttat aaccattctt gtactcctcc
    32641 tcactcaata tattctcttc ccaatactta gcatattcat cttttttaat tacataatca
    32701 ggtaatgttt ctggtctagg acaaaaatca aaactattta atatcgttgt agagttctta
    32761 tccataccaa taatctcctc tatatcccta ttcactatct cctgtatata tgtctctggt
    32821 atatcctcaa atgtaatact gctaactcca tatttaattg ggttaatctt ggtatattgt
    32881 atgttaataa ctatgggttt taactttttg aattgtaaaa tatcattggt aaaaccgaag
    32941 acctcctctt tctcagtaat tgtgtactcc tttaactctt cgtcctttgt aattattaat
    33001 gacactaaac ttggagactc tgcattaggt aggatctcta aaattaaggc cttacaactt
    33061 accttcaaac tatcctcgct ctcttccaca tcgcagtact ctaggagttc tggatatcta
    33121 tctacgaaat attcaggtat ctcaaagttt ctaaaaagga agtaagcacc aacccctgta
    33181 cctattatta atacagcaat taaaggtaac gcaaattttt tagacagttt taacatatat
    33241 acttaagtaa cacttaatac cctattcgca ttccattgca aatctggttt gtcatacata
    33301 ctatccctaa tccattctag ataacttcgg tctattcttg caatgtcttc tagtgtttta
    33361 cctccatact ttccaaaact catctgacgc atgtattgtg gctccttaga tattgctaac
    33421 atactctctg gtgttagctt actgttactt tgcatgtaat gaaaaagatc tcttagaaaa
    33481 tgtacatcac ttaatgcatc gtgagcggta ttgtggtttc cgttttcttt cttgtaaaga
    33541 cctaagtagt agcgaagata ctgtagagag taactctcta ggtcattgtc tccctctgta
    33601 aacatattcc tagctagttt caatgtacat atggtatgtg gtagtttaat accttttctc
    33661 tccagtacct ctttgtcaaa gtctatgtta tgtgcaaccc aaatatattt ttctgctaag
    33721 aagttaagat attcaattaa tgaatttcct ttaaactctg gatcaatatt ctcctttggc
    33781 aattctgttt gctcaaatgc cttcttgtcc tctaccatct ctggggttat gtggtttaca
    33841 gccatggctg agaaagatat ctcttgagta ggtttgaaaa aaagatttaa ttgaacatca
    33901 ctgtccgtaa caaaagctaa ctgaagaaga tctttaatat ttgcatcggt tgtttctgta
    33961 tcgaaataca agaagggctt ttccatatct tttagtatat cctaaatcca acccaaaagc
    34021 atggcagtcc caaggagtac aaataccagt ccaattacga ggtgtatgaa acgaatattc
    34081 ttctctttcc aagaagagat ctgtttaata tcttttgaac caaagaagat caaaaagaca
    34141 atgacaatca ttggaagaac aaaaatcagg ttgtacagag aaagtattgg gacagattgg
    34201 aagaattctc cgaaggaaag cattcctcca agaattatat atggtccaat tgtacaaggt
    34261 agcaaaaaga gtgttacaaa cattccaagt ccaaaagcac caagtggtga agttactttc
    34321 gcaagtatct tttgaacctt aggacgcagg aaaagtggca tctctgtacc tactccacct
    34381 ggtttgtaga agaagaaatc ttttaagtct agtagtccaa gtacaatcgc accaatcgct
    34441 aaacctttgt aaagatacaa acggatgtta ctaaatgatt ggagaacctc aaaggcttta
    34501 acaattaaaa ggccatatat gaagtatgtg ataaagactg cagctatgaa agctagtcca
    34561 gaccagagga tctgtttcct atctttagga tggtatgtcg taactgcaat taacatcatg
    34621 agtaatactg agagtgcaca tgggtttatg gagtctgtta gtgccaaccc tatgaccttc
    34681 agtatggaaa tattactgtc tgtagctgtt tggggttcat cgactgatgt attgcttcca
    34741 gttgatgttc cttgtggtgc atctcctctc caaagtatat tccagctacc tacggatatc
    34801 ccgtaatcat atacttcctc agctccggat attttgattg ttagattgtt agttttttta
    34861 ccttccagtt cttttacata tgcatttaaa tcttgagcaa gaagaaaatc tcttactatg
    34921 ctgttttctt ctggactcaa gtctttgttt gtatcttggt atgctaccct atcctttcca
    34981 tatatttttg gttttccttc aaacttgttt aactctgcag taagcgtatc catgtaccca
    35041 tttgtaagtt caaatgtgtt tactggtata tttgctagct tctcatcgaa gttctttagt
    35101 atatttacat ctccaagatc gtagtttggt tcatcatata agactaaggg tattccaaga
    35161 ccactaccat atctgcttcc atatgtattt aaatacttag catttgcact ttgctggtat
    35221 atctcgtact caatgagcag aaaattccca tctgtattta ccttctcatg caaaactgga
    35281 gttacttttg cacagtgagg acatccaata ccagtaaaat atgttacaga ttttaattga
    35341 ataatttctt gtgcttgtat atttgatggc aaaagaaaga gactcagaaa gagaactaac
    35401 agtactcctt ttaaaatttt cgctatattt actatactgt gtagcacagt ccttaattta
    35461 tcaaatatct aaccatatct caagacacat tcgaattttc taaaaaacag gtagagatac
    35521 tagacagtat acaggagtgg tacagaaatc ctaaacttca gtatctaact tttgggggat
    35581 atgctggaac tggtaaaact actctactgg ggtatttggg attgaaaatc catgatgaga
    35641 acaagaagat tagaattgct tactgtagtt ttacaggtaa agcctcaagg gtattagata
    35701 gaaaattgaa agatgtaaaa gctcttacta agggagatta cacaggaacc atccacagac
    35761 taatttacaa agccattgtg gatgacaatg acaatatcat tgggtgggag aagaaaccaa
    35821 aggaagaatt tgaatatgat ttaatagtag tagatgaggc gtccatggtt acaaaggaca
    35881 tatggaatga tcttctttct tttggagttc ctgttcttgc tgtgggagat catggtcagc
    35941 ttcctcccat tgagagtagt tttaacctca tggaaagacc agttctaaag ttagaagaga
    36001 tatttagaca ggagaaagac aatcccatca tacaggtatc tgaaatggca agaaaatatg
    36061 gtggtattcc taagatggct ttctctgaca cagttaggaa gctttctaag caagatgagg
    36121 acacacaaga atttgtaact gacatctttt cctctttcaa tgatcaaaca atggtgcttg
    36181 taggatacaa caatactagg attaacctta acaaagggat taggcaatta ctagaatttg
    36241 aaaatcaggc tcctacatat ggtgacaggg taatttgttt aaaaaacaat gctaatgcag
    36301 ggatattcaa cggaatgatg ggtacagtag tatctatgtc taaaagtgat attaagggtt
    36361 gtttagatgc tgagatagca ctggacggtg aagatgagtg ttttgttgga ttaattagtg
    36421 aaaaacaatt taacaattca cttgtagatg gaaaaacttt gatgagagag attaatctat
    36481 ttgattttgg gtatgcccta actgtgcaca aggcacaggg tagccaggca agaagagttg
    36541 tagtatttga ggaaaggttt gcgcgtatga cggatgatct atggcgaaga tggttgtaca
    36601 ctgctgttac aagggccgag aaagagttat atatcattgg ttaaagtagt ataattaact
    36661 atgataatta aaaaaccaaa agaagaaaaa atacttagag aagcaggggt aatatctgta
    36721 gagatacttt caaaactcaa ggatactatt aaagaaggtg tgactactct agagatagat
    36781 gaacatgctg gagaattgtg taaagagtat ggtgttaagc ctgctttcaa ggaggtagat
    36841 aactattatt ttaatacctg tatttccata aacgatattg ccgttcatgg gttaccaagc
    36901 aagtatgtat taaagaaagg agatttagtg tctatagatt ttggtattgt gtataaggga
    36961 tatatgacag atcattgctg gacttggtgt gtaggtaaac cagatgaaag aaaaaagaaa
    37021 cttttagaaa gcggtagaag ggctgtagag aatgctgttg aaaaagcaat tgcaggaaat
    37081 catacaggag atttaggttt tgagatggag agtgaagcta aaaaaaatgg tttcaatatt
    37141 cttaaggtgt ttgtaggcca tggtataggg aaaggtttac atgagtatcc agacatacct
    37201 gcttttggta aaaaaggaac aggagatctt ttggtagatg gtatggttat atgtgtagag
    37261 tgtcaggttg tagatgatac agggaagata tctgttgatg atgatggttg gtcagagaga
    37321 acagagaggg gtggagatag tgttatgttt gaatatatgg ttattgttaa gaagaatagt
    37381 cctgttatat taacagatac tagagattgg ggggtggttg tttgaaatta gtaaaatatg
    37441 gtataatata ctataagctt aaatggctta tgcagctctt taacacactt atttcctgtc
    37501 caattctatg tttttgtaaa tctttgtgaa tttatatata taaatctgtt aaggtttaca
    37561 aaaattagaa aaggagaaga aaatgaaaaa actcattgta atacttgttg ttttatttct
    37621 tgcaattcct tcgggaaaag caagtgcaga tttcgtctgc gacagtgctt gtgttgaaag
    37681 aatactttct gggggcattt cttccgaaga aatctcatac tatggcactg gaaacttact
    37741 aagagtacta acttcctatt acggtggtga cacatggatg gcattacgta tgctcaacct
    37801 tgcgtcaaat acgtatacgg cagtgaatac tgtgaactat gtctatggta cacagttcaa
    37861 agctgacact attggatttt accaatgtca aagtaagtac ttatattgtg aggagggaca
    37921 atttcttgga tatttccaag aggatgacaa gatttatagg gtaatctgta ctcccggcga
    37981 aatttgccaa gaaaagatta tccttgaaga tgaaccactc aatcttctaa atgaagtttt
    38041 tttccagagt tgctattatg gtggcccagg agaatgggga ataagttgtg aggaggtgcc
    38101 acgatgaaat atttgctttt tagcattata tcgtggcacc atctcctact cttttcaaga
    38161 ttttgatcta taaatataca gtatgttggc aaagatatct ttgtagtttc gttaaaaaca
    38221 atacacttta tctattatag gtttgattca caaaattatg agttttaata cccataagtc
    38281 ttaagctttt taaaaaggtc tatttatata tatcttctct tagttaatac aaacataaag
    38341 attttatatt tatatattag caaattgtgg tataatatac tataagctta aatagcttat
    38401 gttgctcttt aaaaaggtct attgtctttc tccaattatt acacgaactt ttagctttta
    38461 aatatctaaa ggtttgaagg ttgtaagttc gtgtaataca ctaaaaaatt agaaatcttt
    38521 agaaaaggag attatgatga tttttgatat attaataagt ttggtagcaa cattacatgt
    38581 aggaatcgtt tatattcggt ccttgaatca aacgccgaag acgacttctt acaagcaact
    38641 aataactgtt gggtacgttt taactctagc atttttgttt ctagcaatgc tctgtggata
    38701 caatgatgca aagttaacct atacgttatt cgttgcagtg cttatcatcg cagataccca
    38761 taggatattt aaggaatgga agtcgataaa acaaatggac atatttttcg tccttttggg
    38821 atttctactc ggaacactta tccttttggc aaacacttac tcatatcaat ttctacctag
    38881 atagaccttt cggatggtat ccagacatga tattgccctc aaaagcatat aacatctcta
    38941 cactggatac catctcgaaa aaccctcagc tattaaatca catctttatg tactttatct
    39001 attaaaatat attatagatt tgatttaata agattatgag ttttaataca cataagtctt
    39061 tagttcttta aaaaggtcta tttatatatc ttctcttagt taatacaaac atagagcttt
    39121 tacatttgta taaaggttgt atgtttgtat taacaaaaat ttaacactac acaggagaga
    39181 aagatgaaga aacttataat agtaggaatt atcattttat tgggaatagt ggtagttagc
    39241 tctaaagttt ctaagttaga aagatcaccg ttatgtgatc ggtatagggt aagcataagt
    39301 gcccccctgt acatccctga atattgtata aaaagtggaa aataaaagga gaaaaaatgg
    39361 aaaagaaatg gtcactaatc atcatattga ttgtaatcgt ctcgttgttt ctgtacttca
    39421 ctggatttgc aagtgcagat tcatggtgtc aaccagatga tgtagtttgt ctcgaaaaag
    39481 aaacaaaccc gaaatacatc gcatctttcc aggttatgga ttcaaacggc aggattgttg
    39541 atagtggtca atggaaaaat tcttttgatt tgttttttaa caatccaggc tgtatatatc
    39601 ttcaaccacg cacccaaaca acattttatt ccgattggga tagaaactgg ttttgggaaa
    39661 agaaaactga tctctttgga aacgtttgct acaagcttaa aaaataaata gaccaacaga
    39721 gggtactcat tgcttaaatg caaaatatat tttaattaat atataatgag taccctcctg
    39781 ttcgtccaaa tattgagcat atatgataaa atatatacgc ttaatatata acccccttta
    39841 aatatgccag aaaacaatat atatgatatc gctatagttg gaagcggacc tgccggttac
    39901 tctgcaagca tatacgctag tagatacaaa ttaacaaata ttgtttttgg caaactaatt
    39961 ggaggaacta tcactgaagc acacaaagta tgtaactatc ctggattacc agatatatct
    40021 ggcatagatc taggatacaa attccatgaa caagccaaag aacagggtgc acaacactct
    40081 accgaaagta ttgtagatat taaaaaagaa ggaaacatat ttaaactatt tacagattta
    40141 aacaaagaat accttgctaa aacagttcta cttgctactg gaactgagag aaacaaatta
    40201 gttatcccta aagaagagga atatgttggt aaaggactct cctactgtgc tacgtgcgat
    40261 gctatgtttt acaaagacaa agtagttgct gtagttggtg gaagcaatgc agctacaatg
    40321 gctgctacaa tgctctcaga cattgctaag caggtatata tcatatacag aggaacagaa
    40381 ctaagaggtg aaccagcatg ggtagaacag gtctcacaaa agaaaaatat tcaaacaata
    40441 ttccaaacct tagttgtagg attagaagga gaaccgagat tacaaagagt taagctatcc
    40501 aaaccattca atgattcaga atatctagaa gtagatggag tatttgttga aattggtgca
    40561 gaaccaaata ttactctccc tgttaaacta gggttagagc tagatattca aaactatata
    40621 aaggtagatg aaggacaggc aacaagtata gagggaattt gggctgctgg agactgtaca
    40681 accaattcaa acaagtttaa acaacttgta actgcagtat ctgagggtgg aattgctgca
    40741 aattcaatat attgttactt aaaacaagct agttgttctc actagcgttc ttaaattcaa
    40801 cccactcctc caagtaatta gagctagacc atattttggg gttgcctaca ttgtagatga
    40861 ctttccctcc gtatgattct actgtatcta cctctggaac attgtttttc atatctctat
    40921 ctcctccctt agcaaatatc tcgggtttca ttattgcaat agcctcgttg ctatccgttc
    40981 taccctctgc atcgtaaatt acaacgtggt ctacacattt aaaagcatta ataatatatg
    41041 ccctatcttt cgccggcata aaggctttgc ctttcttagc tactgcctgc atatctccat
    41101 ttaacaaaac tacaagaact tctccaagtt tcttagattc ttcaaaaaga gatacatgcc
    41161 ctggatgtag aggatcaaag aaaccagaag taacgacaac tatcttgccc ttgagattct
    41221 catctctaaa tttggggtac tcaccaatat ctaaaatcat agttaatata ttaaatctta
    41281 aatatatctg tagtcaaact catattacca taactaggcc tctagtgaaa tatattttga
    41341 tactgtttag tggttttaac aacaataaca gtttttttac tctctatggg atatttacaa
    41401 accactactc cttgtattag tctagtctac cgtacacaac aggttgtatg gttaaatatg
    41461 agatgtccat tttgctcttg tgataccacg aaagttgtag acaagagaga caacagagaa
    41521 gatagttcta caagacgcag aagacagtgc cttcgttgtg gtaaaagatt tactacatac
    41581 gagagaattg agaaggtgga tatcagtata gtaaaaaaag atggctcttt agaaaaattt
    41641 gatgtcgaaa agatcattaa aggaattaaa cgtgctgtga accaacacag tatctctgat
    41701 tctgaaatct acaacttcgc agaagaagtt gagaagtatg tctttaatag tgaagactct
    41761 gtaacctcaa gagatattgg gttaatgata ctcaattggt taagagagaa agacccatta
    41821 gcatacatcc gctttgcgtc tgtatacaaa gattttaaag atttaaatga cattaaagat
    41881 gagattgcta acctagaaaa aagctaaatt aataacttgc ccgtatggct gcccagaaaa
    41941 aaaagataac actcaaagta agaaaaagag atggaagaat tgtaaaattt gaacgagaga
    42001 ggattgaaac aggaatattt aaagctgcag agaatgttgg aggaaaagat aggcaaagag
    42061 ctgtagagat aacagatgag gttgtaaaga gaatacagga gaaatacaaa ggtaagaaag
    42121 taattaagac agaggcaatt gcagagataa tcaagcaaac actaattgat cagggacacg
    42181 gtaaaacatc catatctttt ggtttgtttg ttgatttaaa aaaccagatt agaaatatta
    42241 aatccctaat tgatgcaaat acattagtta aggagtacat agataacgag gactggcagg
    42301 taaacgagaa tagtaatatg acttactcat ggcagggatt gaataacttc atttcttcca
    42361 ctgtacaggc taattactgg ctacacagta tatatccaaa agatattgct aatgcgaata
    42421 tagataaggt tatacatatc catgatcttg gtatgctggc tgtttactgt tgtggttgga
    42481 gtttagagga tttgcttctt aaagggttca ctggagttcc aggaaagatc tcttcttctc
    42541 ctgctaagca ctttgcagta gcactcggtc aatgtgttaa ctttttctat accctccagc
    42601 atgaggctgc aggagctcaa gcattttcaa gctttgatac ataccttgca ccgtttgtta
    42661 ggtatgacaa gttgtcatac aaggaagtta aacagaagat gcaagagttc atctacaata
    42721 tgaatgttcc tacaagagta ggatgtcagt gtgtgtctga agatacacag attcttactc
    42781 cagacggatg gaagggctac aaggatatat ccaagggcac agttattaaa acctacaatc
    42841 tcgaaactgg aaaaattgaa aaccaggaag caacttctgt ctttaagagt gaatacaagg
    42901 gaattatgta caatcttaag aacagaatac aagaccagtt aatatctcct aaccatagag
    42961 ttgttaggaa gctgtttaat tcagataaat acactcttga gccaattgag aaggttatgg
    43021 agcttaaatc tcccttcatt gtccctgtct caggagagaa cagtaacaag gcaggtagaa
    43081 tcacagatga gcagattaga ttaatggctt gggtaataag cgagggaagt attgaaaaag
    43141 agggttctaa aagaattagt atttatcaat ccaaaattaa aaatccaaaa aagtacaaag
    43201 aaattattga tcttctctcc tactttaagt ttactttcac cgaggttgaa agaactggat
    43261 ttggaaaacc tgtgaagagg cttagattag atgctaaagg ttcaaaaact attatgaaat
    43321 ggtttggtgc agaatcaaat atcaaatttc ttccaaagag tttgtttaat ttaaatgctg
    43381 cacagtccag actctttttg gacacatatc ttaagggtga tggatttgag gaatgtaaga
    43441 ttacaacttc agagcaagag atcctagatg ggctacaggt attagcagta aatgcgggat
    43501 atggatttac agtgcttgag agaaaaccga ccattggcaa gaagaatctg tttgtattga
    43561 gattaataaa gcataaggaa acatatatca aagaggccaa gaaggttaat tacgaaggag
    43621 ttatatggtg tccacataca gtaaacgaga ccataattgc aaaaagaaat ggcaaggtgt
    43681 ttattactgg taatactcct ttcacaaata ttacgatgga tttgatacct tctgggacct
    43741 tggcagagca gaatgtaatc attggtggta agcctcagaa agagaaatac aaggatttca
    43801 gaaaagaaat ggggatgatt aacaaggctt tctgtgagat tatgatggag ggtgacagcc
    43861 agggaagatt gttctcctac cctattccca cttataattt aacgacagat tttgattggg
    43921 atagtccaga ttacacacca atttgggata tgacagctaa atacggtatt ccatattttt
    43981 ctaacttcat aaactcagat atgaagccag atgatgctag aagtatgtgt ctagatagta
    44041 aagaagaagt tctgattaga gatgctggaa atattaaaaa aagaagtata aaggatattg
    44101 ttgaaagata catggatgga aattttgata aagatggatg ggctcagtgt aagaaaagtg
    44161 aaaggttaga agctctttct ttaaatcctg aaactggtaa gacagaatgg actaaagtta
    44221 caaagtttct taaagttagc gatgatttat ctgtaaagat aatcatgcaa gatggaaaag
    44281 agatggtaac ttcgtctggg catctagtcc ctgttctcac aaaggatgga ctgattaata
    44341 aattagcaaa ggatgtaaaa aagaacgatt atctcttaag tcttaaatct tcttctgagt
    44401 tgagtagtaa gtatgaaaat ttgggaaaca atttaattct agatgataat cttgcgaaaa
    44461 tacttgggta ttttgtagca gatggaaatt ttctaaagga gagtagaaag aatatgcaaa
    44521 gatatggaca accaagaggg cttcagttta ctttcaatgc taagacaaaa gagcatttgc
    44581 atgaaataaa agatctcctt aacaatcatt taaaacttga tactaaagaa aaaaaagatc
    44641 ccagatacaa tagctactat ctgtatgtct acaatgttga tattgcacaa actatataca
    44701 acgcaggttt taagaaatat ggaaaattac ctgacattct gtttaattct cctaaaagtg
    44761 ttatagaaaa tttccttacc tttcatttta agggagatgg atatgagaaa agaagtgaga
    44821 tacatattaa tgacaaagat ttagcaaaag atttagtgat gctttattct ctaatcaaca
    44881 aacctgttat ctacaaagag aggaaaaaca gtcaaacgat atatatacag caccaaagga
    44941 ataagcttaa cagtaatgga ttcctttctg ctccaatttt agcagaaaga gttcctggat
    45001 ttctagcaaa atctacttac ttggtacctg ggttaaataa atctaggatg gttggtttta
    45061 attctttaga gaaatatagt gctcaaacat tggaaagtaa attgatagaa aaaggcgact
    45121 actacattgt cagagtaaag gatataagtt tgaacaaatt aagggattct caggatttct
    45181 ttgatttgga attagaaaaa aaccatctgt ttgtccactc tctgggtaca attactcata
    45241 attgttgcag acttcgtatt gacaacagag aattgaagaa aaaaggaggt gggttctttg
    45301 gagcaaatcc tcttactgga agtattggtg ttgtgacaat taatcttcca cgaattggga
    45361 tagagacaaa gacaaaagag gagtatttca accatttaga tagagcaatg gacttggcaa
    45421 aaaggagttt agtaattaaa agaacattta ttgagaaata catggagaaa ggtttgtatc
    45481 cttactctaa gttctatctt tctgacatta aaaagagatt tggggagtat tttaagaacc
    45541 atttcaatac tattggaatt cttggtatga atgaatcttt gattaatttt tttggagata
    45601 tggagaaggg aataacctca aaagaagggc gtgagtttgc actggagata atggattaca
    45661 tgagagaaaa attaaaagat taccagaaag aaactaacca gctttttaac ctagaagcta
    45721 ctccaggcga gggtgctaca tacaaatttg cgaaagctga caaaaagaaa ttcggtgatt
    45781 tagccataac tgccgcagat attgcagaga aaaaaggaat gattccctac tataccaaca
    45841 gttctcaact tccagtaggc cttactgacg acatttttga ggctttggat ttacaagatg
    45901 aattccagtg taaatatact ggcggaacgg ttatgcattt gtatcttggt gagcgagtat
    45961 cctctgcaga cgccaccaag aatttagtaa aaaaggttgc agagaactac agattgccat
    46021 acttttcaat cacgccaacc tttagtgtat gtccaaagca tggatatatc acaggggagc
    46081 acaagtactg tcctaagtgc gatatagagg ttgggtataa agatggcatg aagtttagtg
    46141 aaaaatttta attaaattat ttactgttca atatatgaag aaagttacaa gacaaagatg
    46201 tgaggtgtat tcaagagttg ttggatacat aagaccaatt actcaatgga atataggaaa
    46261 acaggctgag tggagtgata ggaaattctt taaggtgact gaataggtta ttctagtatg
    46321 gtacaaattg gaggattaga aaaaacatca ttgctagatt atccaggtaa aatatctgct
    46381 gttatattta catatggttg taatttaagg tgtagctatt gccataatcc agaattagtc
    46441 atagaagaat ttgataagga gcattctatc tctgaagagg aattattaag atttcttgag
    46501 ggtaaaaaag gtaaattaga tgcagtagtc ataacaggag gggaacctct tcttcacccc
    46561 gatcttgaag agttgatagt aaagataaaa gagatgggat ttttaataaa attagataca
    46621 aatggattat tgccttctaa attggagaac ctaattaaaa agggtctgct tgattatatt
    46681 gccatggatg ttaaatatcc cgcagaggat taccctatca tgacaggaat accagatgtt
    46741 atacataaaa ttaaaaaatc tataaagata attatgaatt ctggtgtgga ttatgaattt
    46801 agaactacat atgtaaaagg cattcataca tttagatctg ctaaatatat tggacaactt
    46861 atacatggag ctaagcttta ttacattcaa aatttcagag atgggaaaac aataaccaaa
    46921 ggctttgatt ccagtaactc ctttacagac aaagaattga aacgaataaa aagagaagct
    46981 ggtttatatg taaaaaagac aattattaga taattggttt ttcaagtact tttatgacct
    47041 ttccatttaa catactctct gtgtttttat ctagatagat tggttgatga attggattgc
    47101 ttgaatttgg atataacaca accatatctt gatccttttt aaatcttttc acagttgcac
    47161 aattctctac gatggcaacc acaacatcgc catcaaagat ttcagcttcc ttatctacta
    47221 ctaaataatt accatcagtt atggcctttc caccaatcat ctctctatcc atagaatctc
    47281 ccttcacaat tagggaaaac aggcttttct tcttgccaag aatattttta tcaactcgca
    47341 aaacgccgag gttttcttcc tcggcgccta cgagcggtgt tcctgcattt gcatacccta
    47401 atattggcac tcctattaaa tattcgtata ccggctcttc attttcttct agcatccgta
    47461 tacctcttgg ttcgctagtt ctcaaaatgt agcctttctt ctcaagggcg attagatgat
    47521 ttacgacccc tcttttagtc gctatatcga gaagaacttg tagttcacta agagatgggg
    47581 catatccgtt ttttagaaaa tagtctctta taactctgag taccaaatcc tgctttgcag
    47641 tgagtatact atccatggca gttttatttg tttaatttat atataccgag tataccctcg
    47701 gtctaagtaa cctgcaagta cctgtagtga atgatattgc aaatacaaaa tataacgcct
    47761 atacttatag tcaatataaa ttaataaata atttacatat ggcagaggag aaaaaggcta
    47821 aaaagatcta tactctagag gaaatcaagt ttaacgaggc taacaaagcc atgtctattc
    47881 ttgcatgtat tcctattgta ggattgatct tgttgtttgt agaaaaagag gacaaatttg
    47941 tgagatatat gggagcacaa tttacattgg tcggtgtagt acaactattc acctgggttc
    48001 caatagtagg atggatttta ggacctttag cattggtatt agtcataatt ggtatgatta
    48061 aagcttcaca aggtcaaaga tttgatgtcc cagttctatc tggtttagga ttgaaattaa
    48121 tggctgcaat ttaaagagtc attgaatatt tcaaagaagg gagattaccg tctccctttt
    48181 ttctttacga atggtatgat aaaatcaact tgcactaaat cggggcgtag ttcagttggc
    48241 tagaacgctg cgtttgggac gcagaggtcg cacgttcaag tcgtgtcgcc ccgaccatct
    48301 tgcaaacagt ttccaaactc aatacaatat atacatagtt aaattaatag ataaaacttt
    48361 atgctattgt atataattct tggtgtccta gctgttttaa tcattgctgt aattggaatt
    48421 tacaacagat tagctaaaca gaaagttatg atatcagagg catctgcaga cattgaaact
    48481 ttccagaaac aaagatacga tatgatccca aatcttgttg agattgttaa aggatatgct
    48541 aaacatgagc aagaaacatt tacgaaagta actgagatga gaacaaaagc aatgggtgca
    48601 acatcttttg atgaaaaagc aaagttagag gatcaattaa gcaacatgat ttcaaagatt
    48661 tttgcagttg cagaaaatta tccagaactt aaagccaata ctaacttctt aggtttacaa
    48721 accaatctta aagatcttga ggataatatc caaaaatcca gaagatacta caacggtgct
    48781 gtacgtgact ttaacaatat gattgttgtt ttcccaacaa atattatagc tcaagtactt
    48841 ggattcaaag ctgaaccatt ctttgaagca tcagaggatg agaagaaaaa tgtagagata
    48901 aagttctaag ataaaatatg aagaaattac ttgggtactt actttttgta tttgttctgt
    48961 tttcgtgttt tgcaaccaat gctctagctc aatcaacaga tgtaatctct gaatttaaat
    49021 ccaacattac attagaacaa aatacagaca ttaaagtaga ggaagaaatt acatacttct
    49081 tctcataccc aggacatggc atctacagag atattccaac aaaatatagt gttcaactta
    49141 cactcgctag accaatagca ataaaattag atgagctgta ttactacaag aaagatgaac
    49201 catcaattaa gtacagtgct tacgaaaaaa gtctagaaaa cggttataca agattcagga
    49261 ttggagaagc agatacttca atagagggag agtatgtgta tgtcataaag tacacaatga
    49321 aaaatgctgt aaattatttt gatgaccatg acgagcttta cattaatatt gcgggtaact
    49381 actggaatgt tccaatacaa aaactttcaa cctctattaa agttccagga aagataataa
    49441 ataaaatctg ttatacaggc gtaagtggtt ctaaagaatc aaattgtaca ttcaaggata
    49501 tttccgaaac agagacatcc ataacattag cagatacatt agatatagga gaagacttaa
    49561 cagtagccat atctatgcca aagggtacct taaaagatgt tagaggagcc cagacagtcg
    49621 cttttcttct ctccaatatt ggtcttctgc ttccaattcc tacattaata gttgggctga
    49681 tattaataaa aaagaatgga aaagatagga aattaactac aattccacat tatgaacctc
    49741 ctaaggatat gtttcctatg cttgctggag caatatataa caaagctctc acacctaaac
    49801 atataactgc gcaaattgta cagatggcaa tagatggata tataaagata aagcaagaag
    49861 gtaaaagaaa ctatgtatta gagaaagatc aaatagaaaa gaatattcca gaggatactg
    49921 ttagagctct atacgctgat ttgtttaagg gtaagaatga ggttaatatc aacaacatac
    49981 cctctgattt ctatactact gtaaactcat tgaagaaaag tattgacact aaattatacg
    50041 aagaaaagta tttttccaaa gaaagcaaaa aactatggaa taaattcctt ctcattggct
    50101 tctttggaat ttttgtaacc tttatggctg caacccctct tgcttctata gctgctactg
    50161 gctggacaat aggtatagct atatcttcaa ttattctagt aatccttact tcgaggattg
    50221 aattgcgaga tacactagga aataaagttt ttgcagaact agaaggtcta aggatgtata
    50281 ttaatacggc cgagaagcat aggattgaat tccataatga cccagaaaag taccttggtg
    50341 tgtttgaaaa attgctcccc tacgctatta ttttcggatt agaaaagaaa tgggcaaagg
    50401 aatttgaaga tttgtacaaa gaacctcctt cttggtatgc aggagatata tctaccttca
    50461 atacattgat acttgcaaat tcaatatctt ctgtaagcag aggtatacaa aggaattcaa
    50521 ctcctcctag tagtcattca tcatttggtg gaagtggatt tagtggcgga agtagtggtg
    50581 gaggatttgg tggcggtggt ggtggaagat ggtaattacc aactaacagg ataacctttc
    50641 agtactgttc tgagatatga gtacatatag taaaggttat acattgcttt attgatagca
    50701 tagtctctac tgtgcatata tgtaactctt ttacctccta aactcctttt aaatgcactc
    50761 acaccagccc atggatgctt cttaccatca tatccctctg gaaccatacc accaaataga
    50821 tctagtgttt tacaacctag ctttttagct aattttacac attcccaata caataggtat
    50881 gtaattctga tattcttgtc tctaaacaat gtatcactcc ctgcttggta tatatgcata
    50941 tatttcccat atacagtagc ttgccatata ctaattggtt tgttttgata atatccaaca
    51001 accatataac aactaccagc atcaacttgt aattgtaatt ctctaagcaa cgattttgtg
    51061 gatctatctg tgtatccctt cttttgcata gtttggttgt ggaaatctgc aaagatacca
    51121 aattgtgaat tatccttgaa tatctttaca gatatatccg tattcttagt taacttgtta
    51181 atattgtttc tagtagaatc actcatgtca tgccttaact cttcttctgt tttacttaaa
    51241 tccacatatt gagatatcat ggcatcaatg tcttggattg gactctttct catccctagt
    51301 tttctgtaca atccttctaa taccacattg tctacataca agggggaaat tctaaacata
    51361 aaacatccat tctcttgagc aatgttttta catttttcca aaactgctct ccatatctct
    51421 tcgctattat ctttcaacat aggagagtgt ttaaactcta aatattttcc aaacagtttt
    51481 gtctttccaa aactaccaca cattacacct tgaaacatat ctccattgta aatggcaaat
    51541 ctaaaagatt tctcatttat ttctttgttg tatttgtatc taaagctact tagtagaaat
    51601 gagtaattgt ctaaagaaag aacaagtgaa tcccattgtg aatcgttttc tatttctaaa
    51661 aattttaaat ctagcatatt tctatcttta aattttaata cttataccaa ccttatttac
    51721 tattctagca atatctttgg caatttcttc tgttacttca atatttgttg gaagattaat
    51781 tatatattta gaacatagtt ctgccttcat acaactggct ttaatatacc ctactctttc
    51841 aaatctttca aaaagaggaa acacaacaga agagtaccat cttccaccga ttattttctc
    51901 ttttgccaag gctgctaata cttctgctgg attagagacc aagacaggat atctgagtaa
    51961 acaacctgaa tggttatcaa atttcatctc tgtttttagt gtttcatcgt atatcttagt
    52021 tattattctt ctgtgctcat caaatttttt aatttttggt aattgattga gaagaagaga
    52081 ggcttgttca ttactgtatc ttcgtatatc ttttttacta ccaatatctg ctgaagcctg
    52141 ttttttaatt ttaccaagga atctataaag aataatcttg atttttccaa gtgtaagtct
    52201 ttcatagaat gtaaatatgc ttttaaaata ctcttttttt atctctcccc atctacaaat
    52261 atattctgca tcgtatttag cttccctctc tgtttgttct ggtattgcat cgtaaaggat
    52321 agaaaccttt tttgaaatat tttggttttt tacaacaatc aaacctcctt gagtactact
    52381 tatgctcttg tcttgggcaa aactaaaaag tgcaatgtct gagtatttta aaacatcttt
    52441 gtttgaaaga agtgaatgat gtgcacaatc ctctatgatg taaatcttct ctttctctct
    52501 tctctcttta ttaacctcat ccacaagttc tctgactctc tttaggtcaa gtatattacc
    52561 aaatgtatgt tgaataatta ttgctttggt aaaatcattt atattggatt gtagcttgtc
    52621 taaatctata ttgaagctct ttggatcaat atccgtgtac cttggagtag cccccaacca
    52681 aagaattggg gctggaacag ctatgcaggt aaaagcttga agtatgacct cgtctttggg
    52741 agatacccca actgctcgta gggctacata gagagcgtct cgacctctat tgaagagaaa
    52801 tatcttacta tctttaaaca ctttggatag aagatcttca agaaccattc tctcttttga
    52861 aatatctagc tcaccttttg taatcttttt ggcaagaagt aaatcgtctt cctgtgcgtt
    52921 tggtattgag ctaatgaata ctgacattag attttattga ttaaaaataa gcaaggaaca
    52981 tcatctaatc taattttatt ctttctccat tttgctatgg attctgtttt tatgaattca
    53041 ttctcaccca ttatattttg tgctacacat aggtatgtat catcttcaca gacggagaga
    53101 atattctcaa aaatcttttg attacggtat ggtgcttcca tgaatatttg tgtttgacca
    53161 gttccttttg caaatacttc gatgctttta attgcctttc ttaaatctct gttctcttgt
    53221 ggaagatacc cattgaaggc aaagttttgt ccacttaacc ccgatgccat taaggcaagg
    53281 aatatggaag atggaccaac aaatggaact accttaatgt catttttttg agcaaaaatt
    53341 acaagcctgt atccagggtc tgctattgat ggtactcctg cttcagacat taatcctaca
    53401 ttctttcctt ctattagagg ttttatcatg gcttctatct catacacttt tgtatgctca
    53461 ctaagttcat acatctctat ctcttgtatt tttttattta attctatgga agagaggtgt
    53521 gctctagctg tttttgcatt ttctactaca aatatctcaa gtccttttac aatatctagg
    53581 tatggtagaa gaagaatgtc tttcatgggg acatcggtta ttggtgttgg tattaagtaa
    53641 agtattcctt tttgcatatt agtatgttct ttcaaaatta gtatctattt gttctcttgt
    53701 taatttccat attgctggtc taccatgagg acagctgtat gggttctcac aagaggaaag
    53761 ctcctcgaaa aggtttttca tttcttcata gcttagtttc tgtcctgacc ttatacttat
    53821 gtgacaggct actgtagcaa gtatgtcttc ctttttcttg ttaaagtcct tcctaagtgt
    53881 atccaaattg tcagaaattt caaatatctc tttaaaaaga gcctctgtat taccggcagg
    53941 aagcatggaa ggcaaggaga gaatatctat accatactct tttatctcaa aattgatacc
    54001 taaatcttta aaaaaatctt gaaattcctc taggaataac ctctcttctt tagaaaatat
    54061 gattgatgtc ggtacaagaa gattttggat atctagtgct ttctcttccc gtttttgaat
    54121 tttctcaaaa ttaattctct cagcagctgc atgttgatct attatccaaa gtcgctcatc
    54181 tgtaaattca atgacaatat atttatcaaa aatctgaaat atatttctaa tatcttcttg
    54241 ctcaccttca gtaacagtta gtgcttcctt ggaaaaaagt aaactgtctc ttacagagct
    54301 gtactgttta tcaaacctaa tctcacgagt actgtaatct ttactctggg aattagtatt
    54361 gaaagattct cttctttgag caattggaga gggagttgag tctatctgaa atgaaagctc
    54421 cttgtttaat gcgtgtttaa cagcttcttc tatagcagaa tacactctga atggattctc
    54481 aaaacgaacc tcttcctttc ttggatgaac attaacatct accaattcag gattaatttt
    54541 aatatttatg tagaagttta ctttctctcc aaatggcaga tatctagcgt acccctcata
    54601 gatcgctcgc ataacccctc tgtcaattac tggcctgtcg tttacaaata caaattgcct
    54661 ggtgttctta cttttgtgag atgatggatg cccagagtat ccttttatct tcattccatt
    54721 tccttcatat ttgatctcta ctagttcttc atgaccatat atctcttcta tcctttcctt
    54781 acaaatatct ccagctttac aattctttag cgcacttaaa gagagtactt cttttccatc
    54841 tttttgaatt ctaaaagata tattagggaa cacaatgaag tagcgattga gtaattcgta
    54901 aatctttctg tattcagtag atgcagattt aagatatttt cttcttgcag gaatattgta
    54961 gaaaagattc tcaaccctta ctgtagtccc ttgctcttta gcagcacttt ttacatcact
    55021 ctgtcctttt tcatttaata ctacctgatt tccaattcct tcatcttcaa atttagaaat
    55081 tacatgaact ttagatactg aggttattgt tgaaagagct tctcctctaa aacccatact
    55141 gagaagagtg ttaagatctt ctatcctgct aatcttggag gtcgtgtgag gaagaaatat
    55201 ctctgggaga tttctttttg gaattccaat accattgtca gaaacttcta ttaattcaat
    55261 tcctccatcc tttattttta tctctatctc tgtagccttt gcatctattg cattatctac
    55321 aagctcttta accacagatg caggtcgctc tactacctct cctgcagcta tctggttaac
    55381 tacatcttgt gacaatttgt ttaatactgg ttttaccata tattcatttt aacacatccg
    55441 tatatctgct aaaatctcca tatggcaaca tttgaattag aaagtaaata tctcgcttct
    55501 aaagatcaag aaaagacaat tttttcactt cttgaaggca tacaggaggg taacgaaaaa
    55561 cagacactat tgggtgttac aggttcggga aaaacttttg taatggcaaa tatcataaaa
    55621 gagctaggca gaccagcact aattctctca cataacaaaa ctctagcagc acaactgtat
    55681 gaggaattta aagaattttt tccaaataat tctgttaaat attttgtctc ttattacgat
    55741 tactaccaac cagaggcata tatcccttcc agagatttgt atattgagaa agaatctgaa
    55801 gtaaacaaac agattgagaa tttaagattt ggagctatga actctgcact aactagaaga
    55861 gatacaattg tggtagcttc tgtgtcgtgt atttacaata ttggaaatcc caaaaattac
    55921 gagaacaaat ctctttcctt ttttgtcgga gaaaatatag atctttcaga gatatctaga
    55981 accctcgtag atatgagata tagcagagat tcctttgact ttactactgg agcttttaga
    56041 attaatggag atatattgga aatatatcct ccgtatgagg aatattccat tcgtattgag
    56101 ctttttggaa atacagttga aaatatttct aaaattgaac caatatctaa gcacaagatc
    56161 tcagatttaa aagagatttt aatatttcca gctacatcat tggtgtattc taaagatgta
    56221 atacaaaatg ctattgagat gatacaaaat gatttagaaa aaaggacttc tttcttaaaa
    56281 aatattggga aaacaatcga ggcttacaga ttggaacaga aaacaaatta tgacattgag
    56341 atgctacgag aggtcggata ctgcaaaagc atggaaaatt actcaatata ttttgatggt
    56401 agaaagacag gagaacctcc atacacactg ttagattatt ttccagagga ttatcttatg
    56461 tttgtggatg aatctcacat cacaattcct caagttggag gtatgtataa tggtgacaaa
    56521 gcaagaaagg ataatttaat tgaatatgga tttagattac cctctgcaag agataacagg
    56581 ccattaaatt ttagtgaatt tgaaaagaaa cagaaaacca caatttatgt ttcagctact
    56641 cctaatgaat atgagataca gaagagcaac aaccatgttg tagaacttct tacaagacca
    56701 acagggcttc ttgatccaat gattgatgtc agaaaaaccg aaggacagat tgatgatgta
    56761 atatctgaaa taagtaaaaa tgtggctaaa ggacagaggg tccttgtcac aacattaaca
    56821 aaaaggatgg ctgaagattt aacctcttac cttaaggatc tagatattaa ggttcaatac
    56881 ctacattcag atatagatac aattgaaaga gtagatattt taagagattt aagacttggg
    56941 gagtatgatg tactagttgg aattaacctt ctaagagaag gtatagattt gccagaagta
    57001 tctctcattt taatccttga tgcagataaa gagggcttct tgcgctcaaa aacaagttta
    57061 attcagacta ttggaagggc tgcaagacat gttgaaggaa gagtaattat gtatgcagac
    57121 aagattacag aaagtatgaa gtatgccata gatgaaacaa atagaagaag aaaataccaa
    57181 gaagaatata acaaggagtt tggtattatt cctacctcga tctcaaaaga aattagagga
    57241 agactagtag aagaagacca gaaaattgtt gataatgatg aatttgttga gaagctagat
    57301 gcaaaacaga aaagattatt gattaaagat ttggaacaaa aaatgttgct agctgcagat
    57361 agtttaaact ttgagaaagc agcagattta agagacagaa taaaagagat taaagctaac
    57421 ttgtaaaatc actttttgga aatattgtag gcttcaaatt ttttaatctt ctagccttga
    57481 tagctgcatc atttattgct ttcttgtatg tctgtttggc aaatagaaaa ttgcgggaca
    57541 tagattcgaa ctatgattct cggcttcaaa ggccggtgtc ctaccattag acgatcccgc
    57601 actacagcct agattataca acaagtggta cttgtaaata aaatgctctt cattgataat
    57661 aaatgtatga aaattttcca gaagaaaaaa cccctcgaca agaatattta catgcccaaa
    57721 aaacacattg aacgaggaat tattaaaaaa agaagaagaa aatttacact atttaaaaag
    57781 aatagaagca caggtggcaa gacaagcaag acattatcta aaggcttctt tactgtatta
    57841 ttagtctttg tttttattgg gatagcatac ctgtctgttt tgtatataaa caaacttaga
    57901 agcacagaca ccaataatga gtttgataac gtcatgatta ttggtataaa cgatgtccca
    57961 gtattcccag aatctacttt tatatttcaa aatacaattg accagccctc tgtctctaaa
    58021 ttcctaagtg agggaaattc agcatacagg cttcctccaa atacttccta ttcggaagtt
    58081 gcacaattct acaacaagaa actaccagag tatggttggg agtttgttct ctctgtttca
    58141 gtgggctctg aggacatgag atatggagag tattgggtaa aagaggacag aggtttaaga
    58201 atatattcaa aattcaacga tatctggtat gaaagtatca cagttgaaga agcacgaact
    58261 ggcttggcac aagaagtaaa agatgaaata gcaagaaacc ttctcttagc agatagtgat
    58321 gcacaggatc tactaccaga tttcccttgg ataattgaaa taccgaaaga gtatgtaatc
    58381 tcttactttg tctctcctta caaaaatgaa tctggtgtta tctttaagaa actaggctct
    58441 agtgagacaa tttcacttac accaatagcg gcaagctcca gtaaggcatt tgattacatg
    58501 cttaacgact atgtaaaaga tttaaacaaa aagaatgaag agaaatgggg agtaaagaac
    58561 acatttatca tttctacaaa tacaggaaat ggactacgag gaagtattgg tatgggagac
    58621 gagcttagag aggtagctgt tatccagaac ccatacaatg atattgcata tgtattggat
    58681 agtaatattt acaaatctcc tttctttgag tatgtattga acaatcttaa gcctcaaagc
    58741 aagattaagt actaagtgac ttaattactt cttctgatac ttttttattc tttatcacac
    58801 tatctatctc ttctaacttt gcattcttta tattttccat actcttaaat ttcatcaaca
    58861 gttttttagc tctactctcc cctattccct ctattttcaa aagctgagag cgcaaacctt
    58921 tcttaattct tgctttcttg tgataggtga tagcaaatct gtgtgcttca tctctgagat
    58981 tgatcagaag cttctcgttt cgtatatata tcctgcttgt ttctccctct ctatgcaccc
    59041 aaaattcatc ctttggctta aacccctttc ttttcaacct tttacctttg cttatcccca
    59101 acagaaggac accacgagga acagctgagg ctacaacaga aagctgacct ttccctccat
    59161 ctatgagcac tacatctgga tgtttactca tcttttcatt tttaaacctt cttgttaata
    59221 cctctttgag cataacaggg tcgttgggag tattactttc tttaattcta aatattctgt
    59281 actcattatt tttaatttct ccattttcat ttactaccat cgaaccgtaa gcctcttgcc
    59341 cttgtatgtt agatatatca taacactcta ttctctcaag ttttgttaat tttaactcct
    59401 tcctaagttc tgagaagacc tttttgtaaa gggattcttt tcttaattcg tatgacttct
    59461 catcttcaaa aaaatcccca gttaaactac tcccaagata ttttaagtct gctatcttat
    59521 ctctcaattc tgctgctttt tcatactgct tttttgagga gtattctttc atatccttat
    59581 ctaatttctt aatgaaggag gttctttttc ctgtgagaaa tttaattatc ccatcgatat
    59641 tctttctgta atcttcctta gatatatgcc cctgatatgg tcctggacaa agaccaagat
    59701 aaaagtaaag acactctttt tttgcttttg tacatgtaca gaaaggatat attttcctta
    59761 aatatgtgaa gactcttttt actgaagagc cacttggata cggaccaaag agtcttcctc
    59821 gtttaattgt atttaagtct ttatttctta caaccttaag ctttggaaat tcttcattag
    59881 tggagatatg tatccaagag taggatttgt catcttttaa gtcagaattg tattttggtt
    59941 tgtatttctt aattagggca gattccagca caagcgcctc aagttcattc tcagttacaa
    60001 ttgtttctat atctctaaca agtggaatca tttttgctac ccacggtcta tcttctaagt
    60061 taccattaaa gtaggaagat actcgttgtt taagattcac agctttgcca atatacagaa
    60121 cctttttatt cttgtctagg aatttgtaaa ccccgggttt gtttagaatt tctttagcca
    60181 tttctttgtc tctgaattta gagctatttt cttctttaga agtactgccc ttttgctctt
    60241 tccgtcatta atgattattc tcacagcagt atccccatct gagctagaaa tatatttttt
    60301 caacaaagca atatcttttt ctttgtgtat gggtttaatc ctaaaggtta caccgtcaaa
    60361 agaggttgtt ctcttatcat cttctacgta ccttgccttc tcgaccacca agcttttgtc
    60421 accatctctt acatttattt ttgctgctat gagaatgggc ttgttttcga caaggttttc
    60481 ctttatttct tggtatattc ttgggaatac aaccgcatcg ctacttcctg ttttgtcctc
    60541 tatcataagg aaagccatta cttcaccctt tctggttgtt atctttctta tcttgtttac
    60601 cataactcca agaataacaa gttcgtgatt ctttttttct aaggcttctc gcagagatat
    60661 gcaattttta gattcaaaaa attcttgtag tgtatcgagt ggatggctag taaaatatat
    60721 ccccaacaat tctttttccc attcgagtgc ttgcttgacc ggtgtctttt caatatttgg
    60781 tagaggagtt tttccaattt tgctcttttt gtctttgctt tcatcaagtg agaagatgtc
    60841 tatttgaccc gccgcaatac ttcttgaagc tgatttgaac ttttcaaaga ctgtaggaac
    60901 aatatttaaa agcgcattcc tgtctccaaa ttcatccatt gttccagaca taacaaggta
    60961 ttctatagct ctcttggtta ttttgtcaga cattctatgt acaaaatcat ctaggtgttc
    61021 aaaattgcca ttcgcctttc tttcatttac tatgttttta ataatgtctt ccccaacatt
    61081 ttttattcca ccaagaccaa agcgaatatt cacatcgtct tcaacttcaa aatagtagcc
    61141 acttttgtta atagttggtg gaagaacatc aataccaagt ctttcacatt cattaagatc
    61201 tttaattact ctgtcaaagt tatctaaatc tccctctaag agagaggcca tgaactgaat
    61261 tgggaagtga gccttaagat atgctgtacg gtaagctatg gtagcatagg aagcactgtg
    61321 ggccttgtta aagccgtagt tagcaaagag tagtagcttt tcccaaagca attcaacctt
    61381 cttagcatca aatcctcttt cttttgcacc ttcaacaaat ttaggctttt cagcttccat
    61441 tacctctatc tttttctttc ccattgctct tcttaagaca tctgcctttc caagagagta
    61501 acctgctaca acctgagcaa tcttgataac ttgctcttgg tatgtaatga caccatttgt
    61561 aactttcaat acaggttcta attcatcaaa gatatattct ggtttctttt taccctcttt
    61621 tacatctgca tattctgaaa tgaactgcat agggcctggt ctgtaggctg caatgatgta
    61681 acagagatct tcaagagttt cgggtttgag agaacgaatt gtccttttca taccttcact
    61741 ctctaactga aagaccccaa tagtatgtcc actttggata agttcaaatg tttttttatc
    61801 atgtaaatct agtttcatta aatcaattgt ctctcctttg cttctttcaa tctttttaat
    61861 tgttgagcca atgatattca aatttctaag accaaggaaa tcgaatttca ttaatccaat
    61921 aggctctaca tcaaacattt cgtattgtgt cattccaaga cctccaccgt gtgcatccct
    61981 ttgtattggt gcataattta caactgggtc tggtgtaatg attacaccac atgcatgagt
    62041 tgagacccca cgacacaggc ctgacacttt tcgtactacc tctgcaagat gtcttgtttt
    62101 ttctgaagaa tttattatct ctgcaaactc tggattatgt tctatcatgt aatctatatc
    62161 ttttgctttg ccaaaaagta tctcaaccat cttagatagt tggtctgcaa tttctaaatc
    62221 cacacctgta actctggaaa catccctaat ggcctgcctt gttcgtagtt tactaaaagt
    62281 accaatctgt ttaacattct cttcgccata tttttcaata gcatactgaa taacttcatc
    62341 tcttctttta tctgcaatat ctatgtcaaa gtctggagga ctaggtcttt caggattaag
    62401 aaatctttca aagtaaagtt cccatccaat tggttcaata tctgtaattt caatagcata
    62461 cgccactaca gaaccacacc cagagcctct catacccact acaatgccat tctttctaca
    62521 gaaagctaca aagtctctaa caacaagaaa ataatcgtta tatcccttgt catcgatgat
    62581 ttttaattca taatcaactc tatccgttaa ttctttatct atctttgtat atttatcctt
    62641 aagaccatcg taggtaagct ccttaagata ttctttctgg gtctttcctt ctggtaaatc
    62701 tagaaaatga ggttcaactc ttccaaaggt tatatcgtaa tcttcaatta aagctactat
    62761 cttttgagta ttggtaattg cctctggaag atctttgaaa agaatcttca tctcctctgg
    62821 ggtttttaca taaaattcat tggtaggcat cgttcttctg tttgggtcat ccattgtatt
    62881 tccatcactt atacaccaaa ggatctcttg aatttctgca tcttctttat ttaagtagtg
    62941 tgaatcacaa gtagcaacaa ttggaagctt tagctctttt gctatcttca tcagtccttt
    63001 gtttgctatc tcttgttcat ccattccatt tcgttgaatt tcaatgtaga aattatcttt
    63061 aaatatcttt gcataacttt tagccctttg gagtgctaaa tcgtaattat cttgtaggag
    63121 tgcggttgaa agtggtcctg ctagacatgc agaaagagag attattcctt ctgtatattt
    63181 agaaagagtt tcaaaatcta ttcttggtct gtagtaaaag ccatccatat gcgcgacaga
    63241 aacaattttc attaggtttt tgtagccctt taaattctta gctagcaaaa taaggtgaaa
    63301 atatttgtta tctatgccaa attccttttg attcatcccc cttggtgcaa catatatttc
    63361 acaaccgatt ataggtttaa gacctgcttc tttcattgca gtgtagaatt tgaaaacacc
    63421 atacatgctt ccatggtcag taatggcgca agaggtcata ccagaagatt ttaccttctc
    63481 aactagatcc gtgattctga ttgtagcatc taacaaagaa tactctgtgt gtagatgtag
    63541 atgtgtgaac ataacaaata ttgccccctt tctggtatac tgaacattat gccagaagag
    63601 attacaagta agataaattc gttactcaaa gtatcattac ataactccaa tattattaga
    63661 attggggttc tcgtaataat actttcttta tattatatcc ttctttcacc ttttgtggaa
    63721 aacaaactag ttgatttatt cgctggaatg attagagaag ctgacaaaag cgaagtaaat
    63781 actttaactc ctgtctttga catccatgtg tatgctggac agtacaatct caaaacacaa
    63841 cttgagacgg gaaattacac tggctcttcg aaatttttca ctcttgatcc aagaatattg
    63901 gcaatgaaca aatttttaac ggattacaat tctccaatgg cctcatctgc acaggctttc
    63961 attacagagg cagacaaata tggcctagat tggagattag ttgcttcaat ttctggtgta
    64021 gaatctgcat ttgggaactt aatcccaagt aattcgaaca atgcatgggg ttggaggggt
    64081 ataaataaaa acgcaagagg ctggagtatg ttcactacat gggaagctgg aattgcagaa
    64141 gtaacaagag gtttagcaca aggttatgga gttactcttt cgccttttga cattgaacct
    64201 gtatactgtc ctccatgtgg tcaaaatcct gaacatgcct gggcaaatgg tgtaactagg
    64261 tttatgaatc agttacaata ttatactaac aatttggaat tattttagtt aaaatatatc
    64321 tattatgtta aaactcatat tcagattagc ctatacaata atacttttta ttgaggcttt
    64381 aattatgacc cgtatagttc tgatccttat taatgcaaat acaggcaacc cgtttgctgg
    64441 atgggttaag aatacaagtg aaatgtttat ttctcctttt gtaggtataa ctgcaaataa
    64501 tctccagata gataactttg tactacccct taccccgata atcgcattag ttttttacat
    64561 tattgctgct ttcattctct cagaacttct aaaatcgttt tctagggagt aaaaacccta
    64621 tgtggtataa tactccaaat ggcaaaaata gatcttaaaa aaacatctgg atttcagctt
    64681 ttctatgatg gagaaaattt acaaagcaaa gatctttctt tcgaaggaaa gatagagatt
    64741 tcgattgcag atgtaagacc acaactgcta aacaaagaac ttacctgtcc tgacattttc
    64801 tatactaaat acaaaaacct tgaccaagag gatttatatc gttcaaaagg tattagaatc
    64861 aatttctacc tacttcaacc taacctggct ggtattgaat ttgttaaaac aagagcaaca
    64921 agagttctta aatacccaag aataattgat gttgtttatg gtggagcaac tattctttta
    64981 caaagataca gaaatccaaa ggataacaga ataattaaag tagttgcaaa gaaagaacag
    65041 aaagttatca tccccgctgg atatgccttt gttatagtca atactagaca gaataccaac
    65101 ctcattttcg cagaatattt atccaccaaa gctataagta aagttgtcct tgatgagatg
    65161 aacggtttag cttattatat tattagaaaa aatgctaaac aggaaactgt ccgaaaccca
    65221 tattacaaaa tagtcaacga accagaaaaa ttggactggg ataaaatttt gtttaaatac
    65281 ggaataacac ccaaaactcc tgtcattaaa caaataatgc gaaaatatga aaaatttgaa
    65341 tggttattta aagaagattc cgtggatatt tagttttgta ctctttcttt ttcttttccc
    65401 aaatagttca tatgcagtag agttttttac aacaacttcc tcttttaaac acaccttaac
    65461 tcaggaaagt gttaatactg aacttgtatt agatataagt tcaaaggaaa ctagagttgt
    65521 ttccttcttt actgcttcaa ttccaattaa aaatcttgat gttaaatgct acaactacaa
    65581 aaccaacaaa aagattgatt gcacgacata cgatagaggt tcatctacag aaattctatt
    65641 taatctaaac aatgcagttg taagacctac cgttcctctg caggtaaaag ttacctacag
    65701 cactccatcc tctgacagta attcatacca aatttcctca tatgtttctg atattaaaac
    65761 ggtaaaagtc ttagtcatat acccaaagga gaaaggtcag ccaatttgga gctcagaccc
    65821 aataacagat attaaaagtg ttggttctaa ttaccagata cagattaata atcccctcta
    65881 cagcactctt tctcttctgt ttggaaacaa tattatctac caatttgaag ttcacagagt
    65941 atttacaaac tctctcacag accaaaacca gacatttgaa ttaatacttc ctccagatac
    66001 acaaaatcaa agtataattt gggaaaagat agaaccgcta ccaaacacat ctcaacttga
    66061 tgtaaatgga aactacattt ttaaatatat tgtaactcca gacagcacta tagatgctac
    66121 tgtgaaaggc catatctaca aaagagacac tacagcgagt gactcactac ctgcgacttt
    66181 ctacacagca cgaatagggt attggaattt aacaaacagc aatgaataca aaagaataaa
    66241 tacattcatt acaaagaagg gattaagcat tccagaagat gtaacttccg taagtactct
    66301 tgaaaatagc caaagagaat tgctatataa atacctgtat caatacacca tagcaaaatt
    66361 agattacaac agagatttgc agttaggaat cacagaacaa accagagttg gagttaatgc
    66421 tttggtagac aacccctcag attcctctcc aattgattat gctgatttct tgattgcact
    66481 atatagaggc tatgatatcc ctgcaagaat ggtggttgga tatgtttcaa atatatctgg
    66541 atttacatca gatggatttt tccactattg ggtggaatat tatgacagtt cattgaaatt
    66601 atggatcact ttagatccat ttctagaaga ttactcaact aaatctcttt acaaaagcaa
    66661 ttttgcagac catataacga ttctaaggag aggtaaatcc cccctagctc caaatcttac
    66721 cttctacgaa gcaaatgact ttcttgttac atctgtacca aatgcacaaa tagttccaca
    66781 atttaaagta gatgccggac tcacttttga gaagaataca gtcacaagac ccttcacaaa
    66841 aggatacata tccctttcaa acacaggaaa tattgcaata acaggatatg agataaagaa
    66901 atcaaatatt gaaaatataa caagatatat agacccaatt aataacatgt cttctcaaat
    66961 agttttacca aaacaaaatt caaacataca gattaatctt ccaatgcaag agaaagatat
    67021 taattttttt gtaaatatta ggttcacaaa tcaaaatata ttctctagtg aagaaatact
    67081 tgaatacgaa atgacaccca cagttcctat acttttggat atttttgtta agattctttc
    67141 aacaatactt ttcattctgt tatcatttct aatatatttt ggtatagtaa aattaaagaa
    67201 gcatggatag atcagtaata ataacaattg taatactcgt agcactctgt ttactcttcc
    67261 ttttagcagc tataaataat cgaaagaaga cattcaagaa gagagctagg atgctcagag
    67321 atttacaaga tttgaaagta ggagcataca gtagtaatcc atcagagagg attactgcca
    67381 ttattaagtt agataatctt cttgcaaaag cactacagta caggtttagt aatgaaaaaa
    67441 actgtggaga aaacctaaag ctcgcaagaa aactttttag tcgtccaata tatgaaaaga
    67501 tatggcaaat tcataaatta agaaatcagg ttgttcatga cgatatctca atttcagagg
    67561 aaaaaatgca ggaagcatat agaatatata atcttacaat aaacaaaata ttaaaatgaa
    67621 aaagattttt ttaatcattg ccctactctt cttacctttt aatctttttg ctcaagagga
    67681 aaatgtttca gtgcaacaga ataatcaata ctttaattta aaattaactc aggtttctca
    67741 atctgtattt gataaatcca ttaagtattc ccttgaggtt acaccactaa aagattctgc
    67801 aaaaacacaa attctttggg aagctcctct ttcatttact gtaactccta aacacaagga
    67861 ttttgtttcc atggctgttg gacaaacata tatctatgag gcaatagtgc atccaacaag
    67921 gggtggagta tatgaaataa cggcttccgt taccgcttgg gaatacgata caaactatac
    67981 aaatactgta agccaaactt taacattaaa tgattcttta gtttctcaac caatatcttc
    68041 agattaccaa gtcggagtta tccttttcac tgtattaatc ctgcttgtct ctggattggc
    68101 tatatgggga gctgtgaagc taacaaagat aataatggtt agaactaaaa aatggcttac
    68161 tccaccctct taatctttta gaaaatagca aatatctttt cctagtgctt cggatatttt
    68221 gaaaagattt gagataggag gatatacagt tcctttttca tacatgctta ttgatttatc
    68281 agataagccg actctcattc ctaactgttt ttgtgaatat cctttagctt ctcttgccat
    68341 tcttaatttt tttgagaata gttttacatt catttgttta gagtgatttc aaaaagttat
    68401 acagcttgtc tataaagttc tcttttttaa atggagcata tcctttatta ataccttcgg
    68461 tagaattgta atatgtattg tatctgtcct gtgggatttc tccacttatg tatggcgctg
    68521 ttttttcttc tcttaatttt tcaattatct tgttcttctc ctcaaggata ttttttaact
    68581 catcgtgttc tactaccata tcctctattc catgcttaag tttgagtatg ttactttttg
    68641 ctaatctttc tctttcttca agctcagagt aggctctccc tttttcaact atttctttgt
    68701 gctcttccga attaagaagt tttcttgtta tttcatctct tttcagtaca ctgtacttat
    68761 acaaagaaag ctctctgctt gagggttttc ttccaagtac cttctcatat acttcttcaa
    68821 tcattgggat tttgtcagag agagattcaa aatcagaaaa catagaccct cctcctttag
    68881 agtatgaatc tgtactatca cctttgtccc acattggaac atcaacgttt ttcataacat
    68941 gattatatta caaattgtgg ataatttgta gttatggagc aggagacggg gctcgaaccc
    69001 gcaaccctct gtttggaaaa cagacattct aaccaattga actactcctg ctttacatta
    69061 ccaatataca gttactcggc tttttcttca acaacttctt ccttaacttt ttctacctca
    69121 tctgtctcgt acatatcctg tatcttgtta atctttaatg ctctctttcc aactctctct
    69181 cttaggtaat acaatttaga cttgcttaca gaacctctct ttacaacctc aatcttagac
    69241 aagattgggg agtgtaactg aacaatcttt tctaccccaa ttccataaga tatctttctt
    69301 actgttaaag acttattcaa accatctccc ttaatagcta tgacagttcc ctcgaaaatc
    69361 tgaactctct ccttgttacc ttctttaact ttaatatgta acttaacagt atctccaact
    69421 cttacatcag gtcttttctt taactgttct tcttcaatct ttttaaatat attgttatcc
    69481 atagttctct ctttaaatat aatattgggg tttccagtgg caattataga ataaaattca
    69541 aaaatatgcc acaatgtata tacatactgc ctatgaatgt taaacaaaaa gtcctactaa
    69601 tagtcctaga tggccttggt gctgcaccag ctagtgaagg aaacgccgtt gtaatggcca
    69661 accctgagaa actttcggcc ctttggagta catacccaca tacatattta cttgcctctg
    69721 gagaagctgt gggccttccc caaaatgtaa gaggaaacag tgaggttggg catctaaatc
    69781 ttggagcggg aaatactgtt atgcaaacac ttccaagaat taacaaagca attgaaaaag
    69841 gtctactaga cagcaacaat acactcactg aagctgtcat tcatgcaaaa agatatgaga
    69901 gcaagattca tctcattgga cttctaagcg atggttctgt tcattcacac atcgatcatt
    69961 ttaaagcggt tatcgatttc ctttctaaaa ctaatcttga aaacgaagta tatatccacg
    70021 ctatgacaga tggaagagat tctcctccac aatctgcact aacatatctt cttgatatcg
    70081 acaaacactg catagagaga gggattggaa agatcgggac attaattggc agatattacg
    70141 caatggacag aaacaacaaa tgggacagaa cacaagatgc atacttcctt cttcaaaata
    70201 atacaggtga gagatttaac acataccaag aggctatatc cacatactac caacagaagc
    70261 ttacagatga attttttcct gctacagtaa ttaatgacag caagattgaa aaaagtgatg
    70321 tcgtaatatt tctaaatttt aggccagaca gatctctaca actcgcaaga gcaatcgcag
    70381 atgacaactt tgttaacttc caaagaataa aaccacaaaa ccttttcttt gctagtatgg
    70441 ttgagtacta taaggatttc ccaaacaaaa tactttttcc aaaacagtac cttaaccttc
    70501 cactaggaaa gattgtagat tcttttggtt taagacagct tagaattgca gaatctgaaa
    70561 aattccctca tgttacatat ttctttaatg gtgggacatc agctatatac aacggtgaag
    70621 atagaataat gattccatct cccaatgtag caacatacga tcttaaacca gaaatgagtg
    70681 ctcctgaatt aacagatata ctcattaaaa gagtctccgc aaatatgtat gacttcatct
    70741 tagtaaacta cgcaaatcca gatatggttg gacacactgg gaacttagca gctggtataa
    70801 aggctgtaag cacagtagat tctcttgtat atgagctagt acagaatttc atctcaactg
    70861 gtggtgcagt aatcatcacc gctgaccatg gtaatgtaga agagatgata aatctggata
    70921 caaaagctat agatactgaa cactccttaa atcctgttcc ctgtataatt gcgggtacag
    70981 atataactca aaggaatctt ccttacggtg ctttgaaaga tatagcccca acaatactgg
    71041 agataatggg tataactaaa cctgcagaga tgaatgggca atcattaatt agaaagattt
    71101 aattcttctt cacaagcttg tatatgcctc taacaatgta tgtaacaact acaactccaa
    71161 gaactacata tataacattc tcatatttat taacataacc ttcaacaatt ttccaattct
    71221 ctcctagcaa gaaacctgca aaagctaata cagaagacca aagtaaagaa ccaagcaatg
    71281 tatacaaaga aaatatccca aaattcattt ttgctactcc tgctgaaaag gatataactg
    71341 ttcttacaat tggaattaac cttccaaaaa atacaatccc attaccatat ctatcaaacg
    71401 ctttataccc cttatccaga tcagacgctg gaataaacat atactttcca aaccttctca
    71461 aaaatctatc aactccttcc ttcccccatt tccctatgaa ataaaaagga agagaaccaa
    71521 gatatgctgc tagtgaagct acaattatag ttacatatat attcatccca tctctcgcac
    71581 aaacaaaacc agagaaaggt aaaataattt cacttgggat aggggcaaag aagctctcaa
    71641 gaaacattgc aattgcgact ccaggataac ctaatgtaga gatagcatta acaagccagt
    71701 caataatagt agaaaccata gctgtttact ttaaatttac ttgaacaccg tttccaatct
    71761 taacaccatt tgtagaacaa aatccagaat ttacctctag cacataacga taggtagtac
    71821 ctgaaaaaat agatgggcaa atatctgtac acggttgcaa accctcttta atatccacta
    71881 caaagccatt ttcatctaca aagattaaat ccaaaggaat aaacatatcc ttcatccaga
    71941 aagatgaatt ggtattctgc tcgaaaacaa aatacattcc ttcataatcc ccaagatatg
    72001 tcctgtgact taaaccaagt tctctttctt ctggactgtc tgcaatttca actctaaggt
    72061 tcactgtctt cccatcccca agggatatct ccatatctct aagtgtaccc tcaatatttg
    72121 cctcaccttt gtcgctctta aaaatgtcgc tgattttccc ttcctcaaca aaagatatat
    72181 cgaaaagatc aaacttgttt tgaagaaaat agaagattcc agcaatgaat ataatataca
    72241 aaagaagctt tagctgatta ctcatactca aatatatcaa aaactaacaa tcaatcaaac
    72301 atttttaaac aaaataatat ataataaccc ttgttatgca gacattcaaa aaatatttaa
    72361 atcctctcta caaacaactt gaggatatag ggtcatttag tgaccttaca gatctttctg
    72421 cactagaaca ggaagagtac cctaccctgc aagaggtaac gaaaaagaag attgctagta
    72481 tacgaagtct tcttccaaga ctagataggt tagaagaaga actagagcat caaattaccc
    72541 tcatagaaac agaaagtgat attgatgaaa gaaaagatgc aaaaaatgtt tacaatcaaa
    72601 tacttaaaca aattgaggct ttgattggaa gaattaacaa cgaattaata gctcttgact
    72661 ctccttactt tggaaaaatt gtgtttttac cagacgactc taagactggt aaacctctac
    72721 tcctgtatat tggtaagttt gcccttgtag atgagaaaac acatgtccca ataattacag
    72781 actggagatc cccaatagca aatatctact atgagaattc tgggcctaca aaaaatgtaa
    72841 gttttcaagc tccagttgga aaaagaacag gagaacttaa acagaaaaga cagtttcaga
    72901 tagcaagagc tagaataaga ggcatctatg atgctaaaag tggtaatgta gcagcagatg
    72961 aatttctcct tgcacaatta aatgaaagac ttggtaaaaa gcttcaagat attgtttcta
    73021 caatacaggc acaacagaat gaaataatta gggaagacat taatcaccca agtgtaatac
    73081 aaggagtagc aggcagtgga aaaacaacca tactcctgca cagacttgca tatcttttct
    73141 acacatacaa agaaactatt acttcagaaa attccttaat cattgctccg aaccagatgt
    73201 tcatagacta tgtctcagat gtccttccgg atttaggtat atccaaggta gatacacaaa
    73261 catatctctt ttgggcaaag agcttcttaa catggggaga taattacaga ctctccaatt
    73321 tagaagaaga tatgaaaatt aaagaattca agggttcttt agaattttta gatatcctag
    73381 atgcatattt tgacaaattc gagaaagagc ttctagagga aatgccatac tctagaaaag
    73441 atattgttgc aaacagatat ttccttctta agaagaatta cctagatata gatatggcag
    73501 aaaggttgga attagcatta gagtattcct ttgcacagaa acattttaga aaatccctag
    73561 atggatatat cggagatatg aacgacaacg atttcgacat taaaaagaag gttttaacat
    73621 actttagaaa taatacctct gtatacagac tttacaggaa tctcttcaaa gatacactac
    73681 tgaatcaaga tgtttgcaca tactcactta atggattggt tagtgaagga aaaaacaact
    73741 tcttcaggat agaggattta gcaccaatgg tttaccttta ccaaaagatt aatggtataa
    73801 aaaatgttac cagggatact gtaatggtag atgaagctca agatatgagc tacatacaac
    73861 ttgctacatt aactaaaatt gctaaggatg gaaatatcac tctagctggg gatcttgctc
    73921 aatccataat tcctcctttt tacattaggt catgggatgg tgtactagat atacttgaga
    73981 gatatactca caaaaaagtt tctttccatc agttacagag gtgttacaga actacagtag
    74041 aaatcataga ttatgctaac ggtttgttta gagagaaatt ccccaagtca taccaacttc
    74101 cagaggcagt attaagacat ggtgatgatg tagagatatt aaatattggt agtacattgg
    74161 atagttgtag tgaaaaagaa ttgaaggttg tagttgaaaa gataaaagag caatttgaga
    74221 agggtgctgt aacttgtagc gtactttgta gagacaaaaa tcattctaca aatatttata
    74281 acagattaaa gagttacaat gagtatttag atagagaggt tattaattac acagagaatg
    74341 attacaaaca gggtctactt gtaatgccta tagagaatgc aaagggttta gaatttgata
    74401 gtgtatttat agctgatatg gattcatatc cggatgatga gttatcatgg aggttacttt
    74461 atgttggtat tacaagggca cttcatagat tatttattat taaatagaga atatctacta
    74521 accttggaac tcattaactt ttacgctaca ataaacaatg cctaaaaaga aactagcccg
    74581 aataatatct gaaatgttta atgggttctt taccatgcta ctaaccccaa tccttgtgtt
    74641 tgtcgtttca gatatcccaa ccatatacaa aatcatattc tccattcttt acatatcagc
    74701 tccagttggt acattcttca tacttaagaa attaggcaaa gttacagact acgaatttac
    74761 caaaagagaa gaaaggcctc tcttctttat catagttact atttccttcc taatactttt
    74821 catcgtaagt accttcattc ttaagaatcc aaccatatcc acagtgacag tagcatcttt
    74881 tactgcctcg acaatactaa caatcgtttc cctcttctgg aaaatgagtg gacacatgac
    74941 atactccaca cttctcttct ttacatgcat attccttttt ccaaacctct cctttctttg
    75001 gttactcttt ctctttacac cagccattgc atggtcaaga gttgagcttg ggaaacatac
    75061 atggatgcaa acagtcgcag gagtattagt ttctactgca atatccatta cctttttctt
    75121 tgttctttga tacaatacct gtattaatgg aaaatctcag tattacacaa ttaagagaga
    75181 aactacttaa caaagatatc tctgcaaccg aagcagtaaa ctactaccta gacagaattg
    75241 caagactaga aaaaactctc aatgcatata ttactgtaaa caaggaagat gctttaaaaa
    75301 aggcaaaaga ttttgacaac aattttgaca aattaaaaga caaaaaactc tctggtatcc
    75361 caatttctgt caaagatgtc ttctctacaa aagatatact aactacatgt gcctcacaca
    75421 tactagacgg atacaaacct gtttacaatg ctactgttgt acaaaggata ttagatgagc
    75481 aagccataat cttgggaaaa acaaacctag accaattctg tcatggctct ggtacagtta
    75541 catcctacta cggacccact agaaacccct gggacaaaga aactctccct ggaggatcaa
    75601 gtggtggttc agctgctgca gttgctgcag atatgtgtac agcctctgta ggaacagaaa
    75661 ctgcaggctc cattagacta ccagcatcgt ggtgtggaat agttggtctt aaacctacat
    75721 attccagagt atccaggtat ggagtacttg cgatgggttc atctttggac tctcctggac
    75781 caattgttaa aacagtagaa gatgcagcat atctcttaag cataattgct ggatatgaca
    75841 aaaatgactt cacatcctcc aaagaacctg taaacgatta ctacaaatta ctagatcctt
    75901 ccaagattaa aggtatgagg ttagcattgc caaaagaata tttagatcta gatatacagg
    75961 atggtgtaag aaaaaatttt gaaagcagta tatccattct caaagactta ggagcaataa
    76021 ttgaagaggt atctgttatg gatcctaaat tcgctatggc tgtatatacc attacatgta
    76081 gaagtgaggt atcttcgaac ctagccagat atgatggcac aagatatggt ctagagggaa
    76141 gtaaaaatga aaatgtaaaa gaatattacg aatctacaag aggagaagga tttggagaag
    76201 agccaaaacg aagagtcatg actggaactt tctcattgtc tgcaggatat gcagatgaat
    76261 atttcaaaaa gtctgaacag gttagacagt taatcaagga tgatttagat ggtgtgttga
    76321 agaagtatga tgcaattgta tctccatcta ctccttctgt agcacttaaa gatgaagatg
    76381 cagataatcc tctttttgga gagattgcag atgtacttgc ggagggaagc tctgaggcag
    76441 gactacctgg tatatctata ccatctggtc tttcaaacag aatgccaaca ggtattcagt
    76501 ttataggaag atattttgat gaacagacaa tacttaatgc agcttatgca ttagagaaag
    76561 aaattggaac attgaaatta aggttgtagt atatcctcac tctcaccatt tatggatatg
    76621 acaaccttgt ccactgtaga gaactgtttt aatgtttgag taatctgtcc cctaattctc
    76681 tcaaccaaac atgctccccc tactccctca cccagttttt cgttgaaatc tacatatgct
    76741 actcccttag atatttttaa tgagttaatt gtaacaccag caggaataga tgaaatatat
    76801 cccttatctt cctctgcact tgttgtacct ttgagaagtt ctaacagtgc agctcttcca
    76861 acagcctctg tctttactac ctttctactt actgcatata ccttttcaca attttctgag
    76921 tttggatctt tcatagtatt gccaaaatac acaagaagac tcattgtgct atctatatca
    76981 caaagctctt taaaaaatat gggtatttcg aaactgtcat catttgctac ctctccagat
    77041 ggattatctt taataaatac taacttcgca tcaccattac agatatctgt gcatgtaacc
    77101 tctgcagtaa attttacaaa ttcatctgta tatgaatctc caacagcttt agcttgtaca
    77161 gtaagaattt ctgcacctgc atcactaaca agtgtaattg gaaaaactcc ttcaaagaac
    77221 catgagccag gtatctctcc ttcaatgtca aaggtacaat ccacttcact atttgggaga
    77281 ggtgttacca ttctgtattc cacccctttg gtagatttgg ttacaatttt tttatcttct
    77341 tcctgagtat taccttgttt attaaagatt ggaatatatt ctaatatctg atctctgaag
    77401 atgtatacaa gaggaataag gataacaaga agaattgcaa tgataaacag aattatgtat
    77461 ttccttttca tatatataat atatacctaa tagaaaaaga aatgtaaaca ggaaatgtct
    77521 cagaatatga ggcgcctggg actcggaccc agcacctact gcttaaaagg cagttgctct
    77581 aaccggatga gctagcgccc ctcataatta cgaaagaatt atatcctttg agaaatatat
    77641 ttacaaggta tatctactaa taagaatcta tacttctagt tcttatacct aacaattcat
    77701 gcttcctttt atgagtaact gggtctgttg taatctttaa tgcaaaatcc gagagagtat
    77761 aagacaaaga atggaccgca cttacatctt tctcttccat aatgtgactc tctttttcaa
    77821 aaagttcatt accaaaacta gaatatatcc ttggacttgc ttcctttccc cagattatac
    77881 cgtagtaact tctgctatta tctggtataa aacctggtaa tccaatccta gattgtttat
    77941 ccggtaccct attagctaga aacaagccct tcattccatc gtcatcgtag agaataatag
    78001 atgatttgct aatatttata ctacgaatag atggtatctc caacgcttct tttaaactat
    78061 tacatatgtg gtttacagaa aatctagccc tatcttgata gtccgcaaga ttaattgtta
    78121 gaacagaatt aggaggcata agttctccaa aaaactcaag attttctctt gctacttcta
    78181 atctcgttcc atcagatatt ttattttcaa catcctcaat catattcttc ctacttaagt
    78241 tatatctgag gatatcgcac cccatagctt tagtcaagag gatattaccc tttgtatatt
    78301 aatctatcta ttggtttgaa acttcttcga tgaataatac aaggtccatg ttttttcaat
    78361 acatccatat gggacttggt accataaccc acatgtcttt caaaaccata aattggatat
    78421 ttcttagcat aatcatacat aatcctgtct cttacaacct tggctacaac agagcttgca
    78481 gatattgaat agtgaaataa atccccctgt gtaattttgt tcattttgta atttggtatg
    78541 gaagatatat tcataccatc tgcaattatg taatccactc tctttccaac cttctcttct
    78601 acaacctgca aagctatact catagcctca agtactgctt tctgtatacc caatctatct
    78661 atatctgctg cacttactat cccatatcca aatgcaaaag atctctccgt tatcaatgaa
    78721 aatgcctcct ctcttttctt ctttgtcatc ttcttactgt cccttacaaa tggtactact
    78781 tggtcaagtg agttaataac tacacaacct gcaaccacag gaccagctaa gggtcctctt
    78841 cctgcttcat ctaaacctgc aactatttgg tatccctgtg accaaagttt ctcctctaat
    78901 ttggtatctg gatatataat catgatatat gataccatta agtatggatg aagttacctc
    78961 aactacaaca ctctcagaac aattacagga acaacctgta tcttctacta catctcttgg
    79021 ttctgtttta attgcatctc tttccatttc cttgttttta gttattgggt atttacttat
    79081 taagtggttt aggttttaat atatatatat ataaattaaa tatttactgc catgtcagag
    79141 gataaaacat tttcatttct taagggtata ttagttggag ccatagctgg agctgtaaca
    79201 ggagtactgt tagcaccaaa gtcaggtgtt gaaacaagag aagatttaaa gaaattagct
    79261 gtagatttcg gagataaagc gaatgagctt tacacaaaag catctactat tcttaaagag
    79321 aaactaactt cacttaaaaa agctggtaaa cagataaacc gagaaagtta tttggaatta
    79381 gtatctcaag tagtggatga gataaaaaag gatggattag ttactgaaga ggtcgcacag
    79441 aaaatttcgg cacaattaaa aacagattgg gctttagtaa aggaagagtt aaagaaataa
    79501 cactatattt aatccaatca taagattaaa tataaataag agagactaga aatagtctct
    79561 ctttctttgt agaataacaa tattatgcga gagtagttca gttggctaga atgtctcctt
    79621 cccaaggaga aggtcgcggg ttcgaatccc gtctctcgct ccagatttcc tatctataca
    79681 cctctcttct gtgtcctatc cgaagaattt ttatttcatc atcatttaca tcgaatataa
    79741 ctctatattc cccaactcta aaacgaaaag atcctgttct ataatcagtt aatggctttg
    79801 caaatagaag tggatcctct tgtttacaaa agaaatcaag tttaataata attcttttct
    79861 gtatagattt ggggagtttt ttgatatctc ttaccgcaga tactgtaaat ctatattcca
    79921 tagtatttac agattaagca ttttcttcac ctcattggca gaataagttt tttcctttct
    79981 tcgtttatca attctttctt taagtattgg ggaagccatc gcttccaaat cttcaattat
    80041 atcttcccac atatcattag gaattaagac ccctaaggcc ttatcgtttt tcaatacacg
    80101 atagaaggta tttgtatcag cagcatcttc aaataaacgg gttagaccag cctgtgcttt
    80161 ttgaatattt gtaaattttt cagaagatat taattgtctc atattacaga agtattacat
    80221 gattctgtaa ttatgtcaag actgtagtat aatatctcac ttccttttta cactcttacc
    80281 tgttacaata ctgttacata tgctgatgta gctcagctgg tagagcactc ccttcgtaag
    80341 ggataggtcg gtggatcgaa gccacccatc agctttttaa aaatatgaaa acacctacat
    80401 acacaattac aaacctaatc ctctcttaca tagttaagta tgaacttgcc atcaaagaca
    80461 taacacacta tcccctacca gaaaaatatc ttctaccttt acaagaaaaa tacggtgcag
    80521 aagatttaaa tgctctcggt gatttagttg gtaataccat tggatacaac aaatcactac
    80581 aagtacaaag aggacaagag ttagcctccc agagaaaaaa tcttcaacta ttcataaata
    80641 tccggaatgc taaagattac ataaattcct acactacaaa tagctctctt aaaccatcca
    80701 tagaactctc ttcacacctt aataaactag caatgaaagg acttgtagat gattgggatt
    80761 tggcaaaatt aaaaacattc tcagaaaaac caaacgagat atatgacaca tggtttaaga
    80821 tgagagacta ctacccacac attgactctg catcgcattt taatgagatt ttcgaatgga
    80881 taaaagatgg aaaagattca aatcacaaac ttattaaatt cgctgccctt acatacgaat
    80941 tcatagacaa agcgccatat tttgtaggaa accaaattac agcaatactt acccttgaaa
    81001 tacttgctaa gcagtatgga tacaatccag ataacttact tcctttcttc aaatccataa
    81061 acaacatatc ccaagattta ctagatgcat ttaagatatc taagagtaaa agagatctaa
    81121 ccaccttcat agaagcattc ctttacgcca tatctcttac tgccatagat gtatccagag
    81181 aattaaaaga tacatataca aagaaagtta aaaaacaggg agccttagag atatcactaa
    81241 acccaagaca gattaaagtc atggattaca taactcttaa tcacaagatt accagaagta
    81301 aatacaccaa gatgatgggt gtatccttta tgacctccta cagagatcta caagaaatgg
    81361 tagataaggg atatatcaaa caaaaaggac aaggaagagg aaccttctat gtcctctcca
    81421 aagaacagga agatattgtc ttggaaattg aataatctag ttaaacttgt taacattact
    81481 aactatggaa tatgacatca tcgttggttt agaaatacat gtacaaacta aaacagaatc
    81541 taaaatgttc tgttcttgtc ctacaggata tttccagaaa gatccaaaca cgcatgtatg
    81601 ccctgtatgt ttaggactcc caggagcgct accagttcca aacagaagag caattgaact
    81661 atgcatactc atgggacttg ctacaaattg taagataaac cagaacatat acttcgacag
    81721 aaagcattac ttctacccag acttaccaaa aggataccag attagtcagt acaaagaccc
    81781 aatctgttca aacggcaaag taaccttacc ctctggagta gaaattgaaa tagagagaat
    81841 tcatcaggaa gaggatgttg ctaaatctac ccatcacaaa gaggtttcta caaacaaaga
    81901 ttattcactc atagattaca ataaatccgg cattccactc attgagatag taactaaacc
    81961 atgtatccgc agtgctaaag atgcaaaaga ttttgccaca caaattagac agatttgtag
    82021 atacttaaat atctctgatg cagatatgga aaagggacag atgagatgtg aaccaaatat
    82081 ctccatacag gagaaaggca aatggaaata tgaagatgga aaaatattac ctgttggaga
    82141 ttacaaatta aataacaagg ttgaagtaaa aaatattggc tccataactg cagtagagaa
    82201 atccatagaa tatgaagtaa agagattaac agaggaaata acaaatggaa aagaaataat
    82261 acagcagaca agaggttgga atgcagataa gggaattaca gaattccaaa gaagcaagga
    82321 aacagcagac gattacagat atatgccaga accagacatc cctgtaattg aagtctctaa
    82381 aaaagatatt gaaagaatat ctaacgagct tgtagaactt ccacacgaga agataacaag
    82441 atatacaaag gattacgata tctctacata cgccgcagaa gttatttctt ctacaaaaga
    82501 aaacgcctct tactttgaat ctcttcttgc agagcttaag ccatctctag atgagaagac
    82561 tgctgcaaaa gaggcagcca attggttaat gggtacagta tattcaattg gtaaagatgt
    82621 agatattaaa gatttagcat tactaattaa aaaatttaga gatggaatca taacaaaggg
    82681 agttgcggaa gaattactta aaaactgtct agaaaacaac aaagatatat cggatgagtt
    82741 agaaaagttt gaaagttctt caaaagaaaa ctctgaaaaa ttggaagata ttgtaaaaga
    82801 tataattgca aaaaatccta aggcagtagc agattacaaa ggtggtaaag aatctacaat
    82861 tggctatctc gtaggacagg ttatgcaatc tacgaaaggt agtgcaaacc caaatgaagc
    82921 gaagaatata ttagtaaagt tacttaaatg aaaatagatg taaaacatat agcagattta
    82981 tctagaatag ctcttacaca gaaagagtta gataaattta caccacagat ggagacaata
    83041 ttacaatctg catctgaatt aaagaatgta gatactgata atatggcacc aatgaaaaaa
    83101 catagaccat tttcagactt aagagatgat atccctactc catcacttac tcaggaagag
    83161 gtattaaaga atgctaaaca tactgagaaa ggatatgtta aggtatatgg aaaaatattt
    83221 ggtgacattg aagaaagtta gtatctctag taaaatactc cctgtataag ccgaggtggt
    83281 gaaattggca gacacgcttg ccttaggagc aagtgggagt aatatccctt gcaggttcaa
    83341 atcctgtcct cggcaccata tattttttac ttacaataca tgctaaagaa aatcataaaa
    83401 ccatttcaag aagtactact tgaacgaaag ctgtgcgttg gatgtacagg acaactagag
    83461 aattccaaaa gattaggaaa cctctcagat aatagattca tggtacagtg caaatgcaaa
    83521 agaagatatg tatttgacaa agaaatgaac aaataccaaa gagctacatt cgcagaagaa
    83581 caacaaatac taaaacagat caaaaaagat agtgaatagt tatcttcaca aagtattcca
    83641 attatagtag aattcttaag gttatgccgc tatcgtctag tggcctagga caacaggttt
    83701 tcatcctgta aaccggagtt cgattctccg tagcggtacc atattatttt atctgcccaa
    83761 accatgtacg aaacagaaac aaaaattaaa actccaacag agaaaatcgc tgcaaccttt
    83821 tcctctattg ggcagttttt actttccttt ttagaaacaa ttgttgtagc actagtaata
    83881 tccattgttt tgtatctgtt tatcatgact cctcatgaag ttattggtaa ctccatgcat
    83941 ccaacatacc agaatggaga atacctaatg gcaaacaagc tattgtacag attccaagat
    84001 ccacaaagag gtgatgtcat aatctttaaa tattcagata cacaagactt cataaaaaga
    84061 gtcatcggtc ttccaggtga caaagttatg ctaaaggacg gacatatata tatcaatgat
    84121 cagctgcttg atgagtctgc ctatttaagt aattcaatct ataccaacgg aggagaattc
    84181 ctccacgagg gagaaagtat cacagttcct gacaatgaat actttgtatg tggagacaac
    84241 agaccacaca gttcagattc tagaaccttt ggcccagtat ccaaagataa tataaaggga
    84301 aaagcatggc ttgtttactt cccattctca caattcagaa tagcccagca cgaaacatat
    84361 tctaacctct agacctcttg cgtgaaacgt ggtaaattga tagggtaaag atgaaaacat
    84421 tcgttatagc aaatcaaaaa ggaggagtcg gtaaaacaac aactgcaatt aatcttggag
    84481 ctagtttagc tctaaagaag aaaaaagtcc tcctaattga cctagatcct caatctaacc
    84541 tttcttctgg aattggcttt acacaacagt ttgacaaaca gaattggagt tcttcagatc
    84601 aaccaccata caaaagtatc tatgatgtac ttgtacaaga cgatcctctc tcctctgtat
    84661 ttgtagctac ctctactcca aatcttttcc tagttccatc acatctttca cttgcaggtg
    84721 cagaaattga gatggtaaat atgctctcaa gagaaacact tcttaaaaaa gctctctcaa
    84781 aatttacaga agattttgac tatgccatta ttgactgtcc accatcctta ggattgttaa
    84841 ctataaacgc attaattgct gcagagaagc ttctaattcc aatccagtgt gaatattttg
    84901 ccctagaggg cctaggacag ttactagaga ctacaaagtt aattaaaggt atcaatccct
    84961 cattaacaat tgggggcgta atattgacca tgtacgacag cagaacaaga ctctctggaa
    85021 gtgttgttgc agatgttcaa tccttctttg gagatgttgc ctttaattcc attgtaccaa
    85081 gaaatgttag actctccgaa gctccatcat atggacaaac aatattccag tatgacaaga
    85141 aatctacagg agcactagcc tacaagagtt tagcttctga gtttatacag cgttttgacg
    85201 attaattttt acattacata atatgcaaaa caacgaaaga ctgggaaaag gattagctgc
    85261 attaattagt acaaatgaga ttgacaatac taatagtgcg tacaatccaa atttcgacat
    85321 aaacaaaata gaggcaaacc cataccagcc tagaatgcat atagatccag aggaactcat
    85381 agatattgca gactccataa gagagcatgg agtaatacaa ccattaatca ttacaaaaga
    85441 caaagattct gacaaatatt ttctcattgc aggagaacga agacttagag cttcacaact
    85501 agcaggttta aaaacagtcc ctgtagttat acaagagtcc tctcctcaag atatgctaga
    85561 gctagcaatt attgagaata tccaaagaaa agaccttaat cctcttgaag aggcatatgc
    85621 atttaaaaag atgcaggaag agtttggcat tccccaaaga gatattgcaa gaaaggttgg
    85681 tttaagtaga gtagcaatta caaacaagat tagattactt accataccaa atgaagttaa
    85741 ggaagatgtt ctaaacgaaa agttaagtga gggacatgca agagctcttc ttggtatcaa
    85801 agacaaagtc tccttaattg cagctgcaga tttagtagtg aagcgaggta tgagtgtaag
    85861 agaaacagaa gccttagtta gaaaaattaa ctttggtaaa ggttctacta ctagaaactg
    85921 gaaacgaagt gatgaagaaa cagataacta ctccagatta ttaagcaaaa aattaggata
    85981 cacagcacat atatctaaaa tgacaaaagg tggaaaagtc gtaattagat acaatacaca
    86041 caaggaattg aaggaattaa tgagtaaatt actttaagca gttacttcct gtcttagctt
    86101 ctcatacata actacagcag cactcgcact tacatttagt gaaccaatgc cctctctcat
    86161 tggtatagat attttttcat ctgctctctc gagtaatcca gcagtaatcc ctacatcttc
    86221 agcacccatt acaaaagcta ctgcaccctt tagatttgtc tcaaaatata cctttccatc
    86281 catatgtaca gcaaatatct taatgttgtt tttcgtaaga agttttagag cattaaaaag
    86341 atttgtttct acaagcggta ttctttctgc agctcccatg gatattctaa ttgtttcgtt
    86401 tgttattaag ttcttctctt gaggattaac aataatgccg tttacaccaa ctgcaaatgc
    86461 agttctcata atcgctgcaa tgttttgggt atatttaaga tcatctaata ctaaaaagaa
    86521 tggctgttta ccttcacttg aaagcttgtc taaaagttcc tctaaatccc agttgttctg
    86581 ggagtacata tatcctaaaa tactttcact atttgaattt ctaagtcttc tggtaataat
    86641 ctttctggag actttcaata cagggatgtt cttcttacct gctatctcta caatctccct
    86701 taccttctca tctttgaaag catttgctgc aatatacagc ttgtcaaaat ctctaccctc
    86761 tttcaaaagc tcaaggatag cattcttact ttctacctgt atgaatcttg tttttgacat
    86821 aattggtatc ttcctagaca gattctacca catctacata gtactgacct cttttaaatc
    86881 cagtcatcct tctaaactgt actactccat ctttgtgagc gtgtattgag taatcttttg
    86941 acatgtatgt attctttcca gaatgataca cagatcctct ctgcttaact attatatttc
    87001 cattttttac aacttctcca gcaaactttt ttaccccaag tctttttcct gctacatgtc
    87061 cattgtttga tgtgcttgct gctcctacta cgtgtgacat tgtcctctat taacaaaatt
    87121 atttaattgc agttatcttc tgcgtagaac aaaaatgttc cttgtgataa tactattttt
    87181 tcgtttccac atcaagtccc ttcccttaca aagttctcca tttacaacat cccatttctt
    87241 gtcaaaaata tctcggatac tgaatgtttt ccatcctgta tgggttttgt aagatgggat
    87301 tatgactact acaacaaacc cttcatgggc aaatctattt aggatatcta ttaatccaag
    87361 atacaaagct ttgactcttt caagatatac atcggctctg tttggaaaaa ggactcttct
    87421 ttggggaggt cccatgtatg gctcacatac tacacattta acttcagtct tttttaaatc
    87481 actaactatt cttgctgggg gatttgtaac atccatctgg aacaccttgt atttgacatc
    87541 tccaatttct cctataccag agagccattc aatatttttc tctgtatatt caatcgcttt
    87601 ttcatcaata tctgatccta gtaagtcaaa tccaagaatt cctgcttcca taagtattgt
    87661 tccagatcca cagaatggat cccaaacaat accatctttt ctacctgtaa gatttaccat
    87721 gattcttgct aatttaggag gaagtgttcc catttctaag tctgtatgtg gcttgtccat
    87781 atctctcttt gcaaaaccat ctacatcttg cacagatatt gtacttccgt atatctcttc
    87841 attctctgtg cgaataatac atagctcaaa ccctttttct aagatactgt tcccaacaat
    87901 ttgtgcagaa tttagtgcac tgcttctgga aagtggatta acaaatctag atggtcttcc
    87961 tacttcttta agaccaacct ttatctgctt accaagcttt ctaagaaatc ttgagtctgt
    88021 ggattctgga ttacctaaaa tgcttaaacc aaatattact ttgtcttctt tcttagagaa
    88081 atcgttaagg aatgtatcta aatcttctat tatgtatcca aatcttatga atccaccaag
    88141 tctgttgaag acttttttaa gcacctcctc ggaaatatcg ttacttttaa ctagaagaat
    88201 attatttgaa tatctatgaa ttgtatcttt gtttaaacca aaggtattaa aaacagaaac
    88261 aagttctgca aaagataaat cctgaaaatt tcctgactca aaatagtact gtttgggcat
    88321 tatcctattt tcttaacaag aacttttgtt attaatggtc ttgaaccgaa atgctttcta
    88381 tatctggatt tagccttata cttaagacca tttatcttct ctcctttttt ctgagaggag
    88441 atttccaaag aaactttaga accttcaata tatgggttcc ctattttaat atcttcacca
    88501 tcagcaatta agagaacctc agagatttca atcttgtctc ctttgttacc tactaatttg
    88561 ttaatctcgt attcttttcc ttcatatacc ttgagctgta ctccagagat agcaattact
    88621 gcaaatttat ccttagaaac tttttttgtt tcttctttaa caaccctctt agttacattc
    88681 ttaggtgttt ttttaatttc tttttctatt tctgccatag gaaacgattt taagttaata
    88741 ttgtcaaaaa gtcaataaat ctaagatatt tcatacacat ctaatatatc ttcacttttg
    88801 ttttttgtct ttctaagata tttcatatat atattctgta caatacccat agaaaatagt
    88861 gtcattatag ttacagtacc acctgagctc ataagtggaa gtggaatacc agtggctggt
    88921 atagctccaa gatttgttcc tacattaata aaaactccaa gaaggatttg taaccctact
    88981 cctatgcata tcaaggataa catcctatct tctaccaaat tcattcctgt tataaagcat
    89041 acaagcacta ttgctgcata caggcctaaa agaaagagac ttccaacaag accaaactct
    89101 tctgcatagg aagcaaatat gaaatcagtt tcgtgctctg gtatgaactc acgtttactt
    89161 tgggtaccat ttccaaatcc ttttccaaat atctgtccag agcctatggc tattctagat
    89221 tggttaacat taaaaccaat attactttct gtttcggctg gattaaaaaa tgcttctatt
    89281 ctgtctttct ggtaatcacg caatacacta tcccatacga cagagagtaa aaccccaaga
    89341 agtaaggcaa cagaaagaga gattacaaca agtattttag acatccttct tgaatatatt
    89401 gaaaagatgg caatcacaat tcccaagatg acaagagcgt accaatacca attacccgtt
    89461 atagctgcca ccaaaaaccc tgcagttata ctaccgatta ttgttaccag cacactgttt
    89521 ctaaaaggat tactaaggcc aagaaatgct agcagaaacc atatactcat aattaacagg
    89581 gacatacttc cacctggttc aagataaatg aggagaaaaa taaccaaagt actcgcaaga
    89641 gtaattagaa agagtatcca ttcattgaat ttctctttca tagacataat gcttgctgta
    89701 attagaataa cagaaagttt tgcgatttca gatggttgta tctgaaatcc acctatgact
    89761 aaccatcttc ttacaccgtt aataactgga gcaaagaata atgtgattac aagaagagct
    89821 acagtaacac catatattag agctaatacc tgccaatact taagatatgt caaatcaact
    89881 ttagagaaga agagaaatcc tagccatcca attcccaaag ctatgacttg tttccaaaca
    89941 atactaagtt ctccaagact tccatctgga agtatcctcg tagaaagaag cgttattact
    90001 ccaagagcag atagaagtat tagtggtaaa acaattacta tgtttgaacc aaaaaaagtc
    90061 cttttcttag aaatgttaat tggcatatga agattttagc acctaatctt tatcctgtct
    90121 tctcataaag gctggaatat ccaaaggatc ttctccatcc tcttgaggaa tgtttattgt
    90181 ggcttcagac ttcttaacag attcatcatc atgctcttct ttctcgtcgt catctctttc
    90241 aaatgtttcc aacacctcat ctttattatc tagaatgtct tcatcatcat ctatatcttc
    90301 tttcttttcc tcttctttaa atccaaatgt tctaagaggt tgtcctgttg ttttctgtaa
    90361 tttagtgtat acctgtttgt tctcgtcaaa gcctgttgct acaactgtaa ttgttaattc
    90421 atctcccgca tctggatcaa tttgtgcacc aaatttaata tttgctgaag ggtctactgc
    90481 ttctttaatt aattctgaaa cctgactaat ctctgagagt gttaaatctg caccaacgac
    90541 attgtaaaga acaccagttg caccctcgat agacatatcc aaaagaggac ttgtagttgc
    90601 ttttcgagcg gcttctgcag ctctattctc tccacttgct ctaccaatac ccataagggc
    90661 agtgccagca ttttgcataa ctgacttagc atcattgaag tctgcattga tatatcctgt
    90721 ttcgcttatg aggctggaaa tgcttcttac accctctgca agcacagagt caaccttctt
    90781 catagcatct atgaacgaaa gcttctcact ttcatcaatt atctctaata acctttggtt
    90841 tggaacaact atcaaggtat ctaccttatc tctaagtgct gcaataccct cttcggctat
    90901 ttcttttctt ttgtttcctt caaaatcaaa tggctttgta acaaccgcta ctgttagtgc
    90961 acccatgttc tttgcaacac ctgcaattat tggtgctgct cctgtaccag ttccaccccc
    91021 catacctgct gtaatgaaaa ccatatttgc accctccaga agctcctgta tttggtctaa
    91081 actttcctct gcagattgtg cacccatctc atggttacca ccaactccaa gtccgtgtgt
    91141 taaatcttct ccaagctgaa gtgtaacagg tgcagaagac atttttaatg cctgtgcatc
    91201 tgtattaaag gccataaatt caactccaga gatatcgtag tcattcatca ttgtgttgat
    91261 ggcattacat ccaccaccac cgactccaac taccttaatt tttgctactg gatctacatt
    91321 ggttgtaact aacataacac tttactcgat aattttaatt aaggcagtaa agacttaaac
    91381 cattctccga ctttttcaaa gaatccgttt acattaacct ttcccttctc atttccactt
    91441 atatctacat catcatctag ggcatgttta atcaagcctt gtacacaagc aaaagatgga
    91501 tcggagatct cctctgtcat tcctgaaaga cctgaaggat atcctactct tgctgcgact
    91561 ccaaatacac tttgcgtcaa tttcgttata tttcttagta atgcactacc ccctgttaaa
    91621 actacacctg ctggcatagc aatatcgtag cctgcttttg aaacattctc ttttgccatt
    91681 tcaaatatct cttcacatct ggcctctaca atcttctgaa tcatctcctt tgaaacactt
    91741 ttctctacac caaaatcaga aagatctacc tcatcgctct tggttttctt ctcaacaggt
    91801 tcatctgctc ttcttaaaag cgcaggagtt gagtccatat ctttaccttt tttaacattg
    91861 catttgttat ctaatatgtc tgacatgttt acctttattt tttctgcatc ctcaagagat
    91921 acctgaagac caattgctat atcactggta atactatttc ctccaagagg aatagatgag
    91981 ctataaatga tagcaccctc ttcaaatatc gctatacatg ttgtaccagc accaatatcc
    92041 attaatgtaa cacccaattc tttctcagta tccgtaagta ctgaaagtga tgatgcccaa
    92101 ccattgaaga ttacatcata tacatgaagg ccaatctgtt ctacgcactt tttaatattc
    92161 tgccagtaag aatttggtgc agttatgata tgtgtttcta cctctaatct tccaccactc
    92221 atcccaatgg gatatttaat tccaccttga ttatctacta caaattctct agggattata
    92281 tggataggat ttaaattgtc tggcaaggaa agtgttctag cattttcaat tgctctgtat
    92341 gtatcgtcga ttgttatttc actacctgca acagctacta ctcccttgtt atttaaactt
    92401 gtaactagct caccatttat tgaaacatat acatctgaga ttgtgacacc tgccattctc
    92461 tccgcagccg ttacactttc agatattgcg ttagttgcat catctatatt tactaccaca
    92521 cctttcttta tacccttact cggatatgta catactccga taacggaagg tgttttatta
    92581 tcctctacag aggatattac tgttgtaatc tttgaggaac ctacatctat agctgttatt
    92641 atttttcgtg ccataatctt taaggcagta attttacatc aaattattat attgaaagaa
    92701 caacactaaa caagttctct ataataatat aatcaaaatt atatatatac gcaagaactt
    92761 tatcaattct taatcactgg tctttcaaat cttacatcca atgacttgaa cttaatctta
    92821 tcacttttga ttttccctgc aactattact aatctctcaa gttgtgtatc taaatcttca
    92881 cccaatgaaa accgcagtat cttgccatct attgtctgca gttccaaaat atctccactc
    92941 agagtaacac ttttaatatt ataacctagc gcttcagtta cctttgtaac tttggaaata
    93001 ctttccataa ccaataattt ttctttaccg ttttctaatt tagatatatc tacagaggat
    93061 gatgtaagaa gatatagttt gtttgtttcg atatagttca tacaacagtc tctagtttcc
    93121 ccttcacata ccctctcaag cataattcct tcgttagaga gtacatagca ttggtctcca
    93181 tttaaaacag aaagtaattc tggttggtat atttcaacaa agatttctag tttatttgga
    93241 agagattttt taacatctgc acttttaatg taggagatgt tagatactaa ctgactattt
    93301 aattcactcg gagagagtaa aaagaagttc tcccctacac tattctcttc tatgtatttt
    93361 gaaatgtcgt taaggatatt ttgtggtatt tcagcaccag agatatcata tatctcatac
    93421 tttttaatat tccagatacc tactaaattt gtaactataa ttaagagtat tactatggtt
    93481 acgaagaggt tcttcctaat aaatgcaact ggtttaaatc tagatacttt atctaatata
    93541 tggttagttg gctgttttgt aatttccata tatttagttt atccattatt taaaacgatt
    93601 ggaagagtgg tacaaaatac tatgggagtg gtataataac aaaccgttgt tatagagaaa
    93661 atagatcttt aaaaagagag gatagggaga aaatggatat aatgaatctg tacaccttta
    93721 gctgttgggg gtaccttcat cctggagatt tcggtggttg ggttcatgga caggagatta
    93781 aaaaaggaat ctcattttgt tcctactatc ctaaagatgg gcttgccttt ctttcaccat
    93841 gtggagcact tcttctggag aatggagaaa aaattacgac ttcaaaagta ataaaccagg
    93901 catatttctc agcaaaagaa cctgaagatt ttccaacgag acatatctgc agaaagcact
    93961 gtaaaaaaat tctgcggact ttaaccaaag aatatgccga tgtaaagaat cttcttgtct
    94021 tagtaacatt cgaggatgaa ttgttcttga ctttcgaaga tgtacttaca cgtttgcgtg
    94081 tgaaagaaga ttcaatcgtc aattacgaag caaatcaaat tgtaacaggc aatggaagaa
    94141 gtgaagacta ttatgttcgt tacgttatct ttcagatgcc aaacaaacat cgtaccggaa
    94201 ttatttccaa cggtaaatat ctctacaacg acaacggcgt actaaaagta ctaaactgac
    94261 ccaatagggt tctatgataa ttttcatata cacatcatag aaccctagag agtccctttg
    94321 tgaatattca atagaaaatt tgtttggagc gagagacggg gttcgaaccc gcgacctaca
    94381 cattggcaat gtgtcgttct agccaactga actactc
//
LOCUS       DAOQWP010000002        48195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_825_length_48195_cov_8.150706, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000002 DAOQWP010000000
VERSION     DAOQWP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 48195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48195
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_825_length_48195_cov_8.150706"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PKI16_00515"
     CDS             <1..219
                     /locus_tag="PKI16_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HNR52958.1"
                     /translation="TIINDEIGSWKVEDLPISKRSKTGLLAGNYKTVADLRSTTATDL
                     LNLPGFGNKSLNEVLELLNQYGINIRGI"
     gene            222..740
                     /locus_tag="PKI16_00520"
     CDS             222..740
                     /locus_tag="PKI16_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HNR52959.1"
                     /translation="MYKGVKKSKLGRKASNRNLLLINLTRSLFKSGQIVTSSARAKAL
                     KQTVESLISKMKTPKNDLVLRRNLQKIFGNDELVKIAMEYSKKEKTGVVIRKVGFRKG
                     DNTEESRIELIGLAKKKGKVVVKKTKEDEKKEGKETEINRVDLEKRSTQKSIDKSAVF
                     KKAERAKSRSGL"
     gene            757..1191
                     /gene="rplM"
                     /locus_tag="PKI16_00525"
     CDS             757..1191
                     /gene="rplM"
                     /locus_tag="PKI16_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNR52960.1"
                     /translation="MKYKTTWTKKEDVKRNWYIVDVKDQILGRAATKIASLLIGKEKV
                     NSVPNLDNGDYVIVINSDFIKLTRGKEETKVYHTHSTYPGGHKETSFRKMLKENSPKI
                     IENAVLNMLPNNKLRDTRMPRLYVYPQAEHKNGGQKPIEIKL"
     gene            1204..1596
                     /gene="rpsI"
                     /locus_tag="PKI16_00530"
     CDS             1204..1596
                     /gene="rpsI"
                     /locus_tag="PKI16_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNR52961.1"
                     /translation="MEKYFESIGRRKRSTARVRIFEGTKASTVNGKALVDFVKGSNKT
                     DLYIERPLIVTGLEKKYYFSAHVNGGGITGQADAIRLGLARALYDMNPDLKTILRKED
                     LVTRDRREVERKKYSQLKARKKPQFSKR"
     gene            1661..2545
                     /locus_tag="PKI16_00535"
     CDS             1661..2545
                     /locus_tag="PKI16_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNR52962.1"
                     /translation="MNKTLKIALISGASLILLSAISIFVLIPLLKGIYHQIRYISPND
                     VTVDGGLTPEELPNSSGKVTDSVGQAESRESALKVIRVGNISISVDDIDATIGQVNTT
                     LEKYKGETLGMQDSGKGKDRIVSLTLRVKEVDFDAFYQELKDLDGEYEYSTISSSDVT
                     ETVLDMEARLKNLKSVETQYLAILKKATTVSDTLAVQRELTTVRSEIEYLESSLDNME
                     RQTDYSTVYLTITQSSTGSSLTDEDWRPIGILKDASRALVSFAKFLGTAVIWVLVFSP
                     VVAAVVIPVVLIQKKAKK"
     gene            2560..2866
                     /gene="rnpB"
                     /locus_tag="PKI16_00540"
     ncRNA           2560..2866
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKI16_00540"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3539..3997
                     /locus_tag="PKI16_00545"
     CDS             3539..3997
                     /locus_tag="PKI16_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR52963.1"
                     /translation="MKKNRNNTIYKPNSYYHIYNRGNHKKNIFLQEKDYKVFRGLMYK
                     YLRKYKILLVAYCYLPNHYHFVFKCADYWQVIPKLMGAFMTSYVMYFNRKYSKVGHLF
                     QGPFGVRRIVGNKDLHGVIAYLRRNPVEAGLVSEENVDSYQWLHIRKQIP"
     gene            4091..5125
                     /locus_tag="PKI16_00550"
     CDS             4091..5125
                     /locus_tag="PKI16_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNR52964.1"
                     /translation="MKKMSSLFIKLKQHKLDNHFLYLIPVIIVTLGVIFSVRIFAEQS
                     GGSPVSNENSRIADINTSLTTLGYGSTSAGTWGDWGSMWNRIRSAAEWAPTGTATAAD
                     VFPGKTFYANGTRSILTGSAVLGKYPEQANISYDDWIAADGTANEYIAEEQTWTLTAQ
                     GGTAASVTDNSITQALSSNKVYKDALTKLYWSDKTVNTLDNEFIWTLGDNRVNPTNTS
                     CNFNSTGNANEYCDNQDPTNAYTEDNDVSATEFCLNLQLDGDNADADNNGLTGVETDW
                     RLPSQKELMQAYIDGSANNLPNAGNAFWSSTERSNIRDNAWLLYLNNGTTYYTTKSST
                     SYARCVRGGQ"
     gene            5511..5876
                     /locus_tag="PKI16_00555"
     CDS             5511..5876
                     /locus_tag="PKI16_00555"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNR52965.1"
                     /translation="MPLANNESNGKVYIALYKLIKYLYGLVQNFPKEYKYTLGGDILT
                     LAWETLDWVTTANGHINSQKAGYVRSALISFQKLKYRIRMAHELKLITDKKMAYLIKT
                     DEEIIKMLRGWYRWTQGQA"
     gene            complement(5838..7073)
                     /locus_tag="PKI16_00560"
     CDS             complement(5838..7073)
                     /locus_tag="PKI16_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HNR52966.1"
                     /translation="MADSFEELIEAYRSCRGNKRNSNYALDFEFNWESRLCSLEKRIG
                     NETYKPGYSTCFVITYPTVREVFAAEFMDRVVHHYLINKIEPKIDKTFINDSYACRRN
                     HGSIAGALKLRKYLNKITRNQTSKAYFMQLDIKSFFYNIDKTLLYDILINHVKKLKLS
                     EDEFNEISWLSQVIIFNDPSKHYIIKGDPKLFNTLPKGKSLFTNPPNKGLPIGNLTSQ
                     FFANLYLNEVDQFIKRELKIKYYLRYADDMILLSNDKDELFMAKEKIQRFLEDKLILK
                     LKDEKTKLGSVYDGIDFLGYFVKPNYVLSRKRVVSNLKKKLYYFNSGFLINRTYCNEE
                     AVPLHNPPSYDEIATICTSINSLFGHLKWANSFNLRQDIYNNHLGMLKEYLDPKDDLS
                     SFNIKSDLGLSLRPAVPSP"
     gene            7155..10247
                     /locus_tag="PKI16_00565"
     CDS             7155..10247
                     /locus_tag="PKI16_00565"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNR52967.1"
                     /translation="MKITAHKIIKNTLLLVSTLALFTIIGYFAPRIFAAGQSNVLGEA
                     TVMNTGNRIDFNSTTYASNVVISDPDPGNNNRRTISGYAWSTDIGWIKFTSGETAGVY
                     VAYSTGLVSGSAYVVNTGGTLDFDNNGSHVVVDTQTGIFSGYVWSNDLGWIDFGDGDV
                     YVKDSKKPENVSNVDAFNDSSKTITIENTSALYNWNSPYFEWSVPIDPDDTSEYASGI
                     AGYFVYWGTDDTTVPETQGTFQTANNFTSPTLTGTHTYYLRIQAVDVQGNIFTGDLAS
                     YTLFTYHFDGDKPTNVSYISMPSSSFGNISEMFFSWPSSGSVASSDNSAVLGWQYSIN
                     DTNSWTGSTHEDRLGFDYIPFIDSDYTYYFDADKDGPKIIVGNNIIYFRTVDTAGNVS
                     VYVSGGISYGGQAPTFEAEAQVTVTPNSSDSNSFSLSWPVALAGEERTVAKYYYMVNT
                     NPPSSYATLANNSSLYIPVDGTSVSTRMLKGAIKGSNTVYVVAVDDQDGYSSSNVITG
                     TFVLNSSLPDPVLNLSLVDSSIKEAELWRASLSWGEPVYKGDGNLSYHVLRSLNGQNW
                     AEIGTTTGLSYTDIVSESATYYYKVLVTDGTDESKANPTPSASYSVNLKGQYTSPALL
                     VSGIVLTEVAIRHATVNWITDRASDTKIMYGTTSGQYFDEELYNSVQTTDHKIRLNSL
                     QADTIYYFKAKWTDEDGNTGMSQEISFRTDPMPEVITSKVERVGLDFATISFEVKGAS
                     KASVYYGKGYTYTGLKEINTSPVDSKYTVILDELSDGTTYNYKISLTDPEGYSYDSIE
                     THSFTTPPMPRISNVTIQELKDVASPTIVVSWFTNTETSSIVTYTAEEGKTASVDKLD
                     TKMKAGSHSMSISGLNPMTRYVATVEGIDKLGNKATSDEIRFTTATDTRPPKISNIKV
                     QGDLLSRSIQSERSRSAQLIVSWETDEPSTSRVEYGEGTSGIYNYSTQTDSELRTKHV
                     VVISGLTPSKVYSIGVVSVDTAENEANSTPIVTITPKASNTVLETVLNSLGNIFSFFQ
                     "
     gene            10256..12466
                     /locus_tag="PKI16_00570"
     CDS             10256..12466
                     /locus_tag="PKI16_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR52968.1"
                     /translation="MKSLDEEKRKLIDIKEIEKSFQTGDRFTHVLKNISFEVYENEFL
                     VIFGPSGCGKSTILHILMGLEKPDAGIVRFGGVDMWKMNSDDRADVRKRDMGIIYQKQ
                     NWVRSLTVVENIAISAQLLGLPKEEAIYKAEEMLKRVGILDKSDYMPTELSAGEQQKM
                     GLARSLVTDPKVIIADEPTGNLDVKSGQEMIDLLKDLTQNGITVIMVTHNPEYLRESD
                     RTIFMLDGELYKEIITKQSDMDSVLKAIQTDLNDFIQGKGKRKQLENDRSFPITKFVE
                     NDVPQKNFFQSISYFLKFIFYFLINTFTLAIGFLIGKISVDKMKKFKDRMTKVSFSRE
                     NDTHISESINVLDLTEISFQNLTTNKSRTSTTILGVSIGIGIIVFLLSIGYGLENLVI
                     NEVTKIQNQNAVEVSSIVGSNVELNDELLDSINQISGIKESFPLINSAAKSSFNGANT
                     DLVVYGVPVGYLEQSQNIWLAGDSLNDSYNEIVINKEYLSILGGSANDILGKTVDLLM
                     INQSVEIKEDSADASKPYKIVGVIDDGNPSVVYMNINEMKNYVPEGYSQLTVIISDRT
                     KLSEIRKQIEVLGFQTSSVMDTVSQVQSLFKNIRLGLVSLGIIAFTIAIIGMVNTLTV
                     SLLERTREIGLLKTIGMKSDEIRTLFLNESMFMGLVGGIGGVILGLILGTLVSIVISI
                     FSFTKGGEFIMINVLPWYMFIILPLVSSIVGYLTGLYPSNRAVKIPPLDAIRYE"
     gene            complement(12499..13473)
                     /gene="fmt"
                     /locus_tag="PKI16_00575"
     CDS             complement(12499..13473)
                     /gene="fmt"
                     /locus_tag="PKI16_00575"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNR52969.1"
                     /translation="MRDLIKEPVRTLFLGTGWESVATLKALHEDPRFDIVGTITTPDK
                     LVGRKQISTPSEVKEYSLKNRIPVFHTDKKPELYQKALDKFKPELVVCKAFGEIIPKE
                     FLEYPRYKCINVHFSILPKYRGAVPIQKAILDGEKKTGISIMLMSEGLDEGDILKIYE
                     EDILDTDTNLSLRERLVKKSSEVLGDVLEEWIMGEITPTKQNPEEATYCWQKDISKEN
                     AQINWKSMTPEYIERMVRALIPWPIAWTRLSGSLNKNIAGKIMKIFKAELVEIPSEKD
                     PGAIFSYDGKVLFATKDPLISLRAFELQIEGRSKTSEREFLNGIGRDI"
     gene            13521..14537
                     /gene="queA"
                     /locus_tag="PKI16_00580"
     CDS             13521..14537
                     /gene="queA"
                     /locus_tag="PKI16_00580"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNR52970.1"
                     /translation="MKMSEFKYNLPQEKIAKFPPKERGTTKLLVLDRKSEAISHKNYS
                     DIVEYIKQGDVIVLNETKVEKRRTFFQTEKGRTLEVLFLNHTEDGNWDCLIKGGKYVT
                     DGDILRDGEIEIQVVGRKDNGFLVRPLNMEAEDIFEKVGHTPIPPYMKREDNPDDYIR
                     YNTVFARLEGSVAAPTASLNLTSEILAQIQKKGAKTAKIELEVGWGTFAPVREENIED
                     HKIHSEKISVGKNTVKLVNDARKNGKDIWAFGTTVARTLESVSDDNGFIHEFKGNTDL
                     YIYPGYKWKCVDHLITNFHMPDSSLMLLVSSFAGKELIKKAYREALNMDYKFLSYGDS
                     MLVI"
     gene            14542..15579
                     /gene="tgt"
                     /locus_tag="PKI16_00585"
     CDS             14542..15579
                     /gene="tgt"
                     /locus_tag="PKI16_00585"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNR52971.1"
                     /translation="MELKDFGKQYIFLPDATRGAVRFLTTEQLKATGTTAIVTNTLHL
                     LISPGAERMQELGGIKKLMNWDGIVLTDSGGFQVFSLIHSKKWSGKVHENGATFKSPL
                     TGDVYELTPESSIDIQMKIGSDILVTLDDCRKTNLTREEALESVERTIEWARRCKNHF
                     EKEYGGTKETGKLLSCVVQGANYPDLRKRCAQELSEMDFDGYNFGGFVVNNEGQLVLD
                     EMKVVVDNTPKDKFKYAMGVGKPQDIRNAAEIGYKVFDTVLVTRNARHGTLYSSDIEG
                     EVLRISNAEYATDQRPIDSKCDCYTCKNHSRAYVHQMLKVGEATGMTLATIHNLRYYQ
                     RLIETLSQPCP"
     gene            complement(15576..16007)
                     /locus_tag="PKI16_00590"
     CDS             complement(15576..16007)
                     /locus_tag="PKI16_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNR52972.1"
                     /translation="MPRGRRNFKKYSYYHIYNRCNNKEMIIKNSADKQLFINLLYKFL
                     KEVDLRLIAFCIMDNHYHLIVKTRKNPQNISTYMCKVVTSFSMQINRKYQRIGHVFQG
                     RYNAKLLFWKKDLRYAKEYIKQNPVREGLVRKPEDYPWSKY"
     gene            16304..17827
                     /gene="rny"
                     /locus_tag="PKI16_00595"
     CDS             16304..17827
                     /gene="rny"
                     /locus_tag="PKI16_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNR52973.1"
                     /translation="MDTLLGILATVLVTGAGSVALLKYVFKWGDVRKIPEKEIENAAK
                     KMMSKKFEEAEDEIAELREREEKRLREIRKESTEHEEILIEREKALNKRSKILDVKSE
                     ELMQIESQLKEGNKRLENSRAQLKTELEKISGLSRDEAKAKLMKEIEEDLKGYEAKKI
                     RQAEKNIEAIADESAQKILVESMQRVATDYVGETTTTSIKVEDEKVKGRVIGKEGRNI
                     RAFEQMTGVDVIVDESPNTIALSSFDPLRREIAYVALKKLIGDGRIHPGAIEESVRKA
                     KNEISLEIKKNGQILAEEAGWPDIDVGLLKLIGKMKYRTSYGQSLMSHTIEVIRIAEV
                     LAAELGADVDLVKKAALLHDVGKVLTHRIDSPHHHISGQIARKYCLDEKLVNAIESHH
                     MDIEPTSTEAVIIYLADAISGARPGARKDSYEQYIQRIEGIENAAKEVGGDKVEEVFA
                     IRAGRELRVVVKPTLVSDDEAIVMAKKIAQSIEKTQSYPGNVEVSVIRQTRAVEVAK"
     gene            18123..18392
                     /locus_tag="PKI16_00600"
     CDS             18123..18392
                     /locus_tag="PKI16_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006813653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNR52974.1"
                     /translation="MLKKDLIDKIAATGLTKRQAGEALEAVLEAISGSLKHGDPVLLT
                     GFGKFEVRTRAARTGINPKTLAKIQLPATKVPAFKPGKALKLAVK"
     gene            18515..19876
                     /locus_tag="PKI16_00605"
     CDS             18515..19876
                     /locus_tag="PKI16_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNR52975.1"
                     /translation="MIKEIFIPNKKNKYKPYLLKKVSLTIFSIILILVNSFGGILGID
                     QAQASTITPTNIINLTNRERTAAGLNSLKTNSKLSSAALAKANNMFEEQYWDHFGPNG
                     ETPWQFIRGAGYYYVYAGENLAKGFSTAEGVHEAWMASTSHRENIMSGKYKDIGVAVV
                     EGVLLGKRTTLVVQMFGNLTQEVAGVTKTVTPKPSTNTESGEIKSISITSPKGGSIIV
                     DPSVNVKGSVKNITGEYSVSVYEGENLVGETESETSNWEFDKDSDWSEGVHKVKAAVD
                     DLKITSKEVSFTIDSTPPKVTENSIVVSENNGSFVLTLEISPDWKDIQVISGENTISL
                     TNSGEKKIEITIPKESISDGVVLMASDVNGNITQMDISEYFEEEEDKTFLQGLSLLSV
                     DTSDGISIVIVSFMFVLLMIEIFVYWRKGKIREAAGDLFTVGVWWLVIAVALFNGFTG
                     IIY"
     gene            19893..20690
                     /locus_tag="PKI16_00610"
     CDS             19893..20690
                     /locus_tag="PKI16_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52976.1"
                     /translation="MEKLKTKVQNDILYLFLILLLTEIFNSVVMSGDKSVLLTVGILG
                     LLLLETWFIRAFSIYSGKKISKYSDVIVRISLKERFFGYFVLPAIFYISLLAFLFFNR
                     NVVLGHFVLGVSMVLFLILFLNVKSSLNKIYRLENVTKIVFDFLCISILYLLINIFVR
                     IGMSPVYFLLSVGVSSFTLLLFILKLHDKMGFLEIVISFLSAIFITCVTQLAWNLSIF
                     TIPAVGTLAFYLIISIWNIRFSGKVKISDYILPFIYVIVTLTLILNL"
     gene            20692..21396
                     /locus_tag="PKI16_00615"
     CDS             20692..21396
                     /locus_tag="PKI16_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52977.1"
                     /translation="MEENFVLKIGGSVMYDNTLNVNTDLMNKVKDWYVNERRNYKKVA
                     MVVGGGSLSRDMQEKISSTVGGEESLHSIAMSVTQTNAAVLQGYLGDMDIFLPKTLGD
                     AYEFLMEENQKTLLSGGLKIGWSTDMDAAIYADILNVKTVYKISNIEYLYEVDPRIDS
                     TAKPILDISWEEYFKLFNIVDGDNHEANKSIPIDVNCAQFCAKKGISFFICGGRYLKE
                     KNRIQEILKNGTLIHP"
     gene            complement(21393..21848)
                     /locus_tag="PKI16_00620"
     CDS             complement(21393..21848)
                     /locus_tag="PKI16_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNR52978.1"
                     /translation="MEEGYKTTKEGLLKLKEELTYREKELKKKITNTLNEMRNQGDLS
                     ENDGYSMAIEEFHINEEKILEIKEKLKNAVVVKTQDKKKVDLGDTVTLKGDTTLKYTL
                     TSEDEANPLEGKISYLSPIGIAVMDKKIKSKITIETPKGKKDYTIENIS"
     gene            21977..22051
                     /locus_tag="PKI16_00625"
     tRNA            21977..22051
                     /locus_tag="PKI16_00625"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22010..22012,aa:Glu,seq:ctc)
     gene            22078..22506
                     /locus_tag="PKI16_00630"
     CDS             22078..22506
                     /locus_tag="PKI16_00630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR52979.1"
                     /translation="MEKNPVYNEADLKDHLGIWLWLENEKGEILMFYHKKFGCWTIPL
                     EKSDPGETLNEAILRTGTEELGIDIANYEIVHTQGNSYIRDGNRVNVEASLVKVLCYD
                     NEITNMEPDKHLEVDWKSKEFILKLEDSTDAVKALQEYIK"
     gene            22519..23190
                     /gene="trmD"
                     /locus_tag="PKI16_00635"
     CDS             22519..23190
                     /gene="trmD"
                     /locus_tag="PKI16_00635"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNR52980.1"
                     /translation="MKFDIVTIFPESIKEYINTSILKIAQEKGLVEINIHNLRDWAKD
                     KHHTTDDTPFGGGPGMVMKVEPVFEAIKDLKKEGSIVAITTPKGEKLEQSKLVEFSKN
                     TNLHMIILCGRYEGFDQRIHDNLVDYEFSIGDYVLSGGELPALVLVDGITRLIPGVLG
                     NEESLTEESFNDGNLDFSQYTKPAEFNGWKVPDILLSGDHKKISKWRKSKSEELTKEK
                     RPDLK"
     gene            23240..24034
                     /locus_tag="PKI16_00640"
     CDS             23240..24034
                     /locus_tag="PKI16_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52981.1"
                     /translation="MEEQVSPNMVDKKKLRVNFLTVFLILVIIGLVVTVLGMSSGWKF
                     KEFDLKSTFIAKEDSIKQDVSGRTSRFVSKYGFSIDVPNYSDYGLNEFTAEEIEILHN
                     KYEVATDLKNHWYLALADIDTADASSNLGTIVEDVSLTYNLSPEAWNSLQSENEEISP
                     LEILYYRVQIYVYENTKGLTLEKVLSEIRETNDISGAVYGEVTYSEISGEKVAKFTGG
                     NSELFQDNIVAISGDYIYVIEKVVSDKTASESMSVLDSIIDSIKFE"
     gene            24057..24833
                     /locus_tag="PKI16_00645"
     CDS             24057..24833
                     /locus_tag="PKI16_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52982.1"
                     /translation="MEEKTIIEETKKVSKGGVGILSIFFFLLALGLAFVIFGYNTKWT
                     YRDFNFNDIFAPAVVEEAKEEETSGVKNAGWALFTLPQYKFSVEIPDYKLKQKFDGQD
                     VYSYWDVTHAEEYYEQSLYYDKALYEDSVEIMFYPKNLPDSVACGQGCVNEHYINIDI
                     YRNEGGKDLPSVQKIIETNLDKAFDFGGEMGATYNGKLVTKWDRQVWSFSMEFVGGSS
                     DGYIVVTNDFIYYIEYYFSDLPTESKDIADKVLDSIAFFE"
     gene            24911..25375
                     /locus_tag="PKI16_00650"
     CDS             24911..25375
                     /locus_tag="PKI16_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNR52983.1"
                     /translation="MKDKVLLTEEKKEELEKELKHLEGKKKKEVMESLEQARREDVSE
                     DNESLSLVLQIKAELERKVEDIKSLLSNSKIIKKQACDPTKINIGSEIKVNFKGKTTK
                     MKIVSSVEADPLKNYISNESPLGKALMKAKLGDTVKLKVDGNTLEYKVLEVC"
     gene            complement(25372..26658)
                     /locus_tag="PKI16_00655"
     CDS             complement(25372..26658)
                     /locus_tag="PKI16_00655"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNR52984.1"
                     /translation="MDLLQNITALCKRRGIIYPNSEIYGGIGGFFDYGPVGVEMKNNL
                     KRYWWKNIVDRRENVVGIDGAIITHPKVWEASGHIENFGDEVVECKKCHQRFRPEDIT
                     DKCSNCGNTEFTEPRSYNLLFETQVGVLEDSKMKTYLRGEACQTIYLDFKNVLDSTRQ
                     QIPFGIAQIGKAFRNEVTPNRLTFRTREFEQWDLQFFVHPSEMEKWFEYWKEERMKFY
                     KGLYRKQENLRLTQHPKEKLVFYAKDAYDVEYLAPWGWAENEGIHWRGDYDLTQHSKF
                     SGKDLSYLDPNTGERYIPNIVETSGGVDRTLLMLLFDAYEEEKLPNGEIRTVLKIHKD
                     IAAYKAAVFPLVSNKPEIMQKAREIFEYLSNDNKVIWDARGNIGKRYRYQDEIGTPFC
                     ITVDYETLENNTVTVRDRDTMEQKRVKIEDLDEALN"
     gene            26773..28308
                     /locus_tag="PKI16_00660"
     CDS             26773..28308
                     /locus_tag="PKI16_00660"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNR52985.1"
                     /translation="MEDQQKSDLLGQREVRLEKLEQLEKLGINPYPAKSFKQYSNGEI
                     KNEFEKFEGKEVILAGRIVAWREHGKLLFGNIQDQTDTIQLMVRESDLQEDLTKGYLG
                     WDKLALLDLADFVEVYGTIGKTKTGEISIMVRNIRLLSKALRPIPRELKNKDQQFRRR
                     YLDLVIHPDKVKLFERKAKFWQVQRDFLKSKGFLEVEVPVLEAVTGGADAKPFITHHN
                     ALDQDFFLRISTELYQKRLLGGGFEKIYTFGPNFRNEGMDEEHLQEFYDIEWYWAYAN
                     YRDNMVLVKDLLLEIANKVYGKTEFTTRGHTFNLADEWKEIDYTQIIKERFDIDIFND
                     SDEKILEVVRKNNIELTGDINRNRLIDNLWKIIRATISGPAFLVNHPKFVSPLAKAHK
                     ENETLTERFQVIIAGSELGNGYSEINDPRDQLERFLEQQSLRESGDEEAQMLDIDFVE
                     MLEYGMPPASGYGQSERIFWFLENLTAREGTLFPQMRNEIETTTREIYEGKVKFPKIV
                     KKD"
     gene            complement(28462..28535)
                     /locus_tag="PKI16_00665"
     tRNA            complement(28462..28535)
                     /locus_tag="PKI16_00665"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28500..28502),aa:Thr,seq:tgt)
     gene            28749..29123
                     /locus_tag="PKI16_00670"
     CDS             28749..29123
                     /locus_tag="PKI16_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52986.1"
                     /translation="MDSIKDILNQLKKDNEQEEDEKQPSLIDTQQLATNPNKLFGNKH
                     KYVTTEYQVYGLRLAGKLGDKKRATMYIKWAKEKPRAILEMAYSFAIDYPNAKDRSRI
                     FMWKVKELENERKEGKIQEDTK"
     gene            29083..29898
                     /locus_tag="PKI16_00675"
     CDS             29083..29898
                     /locus_tag="PKI16_00675"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNR52987.1"
                     /translation="MKEKKEKFKKILNREVGMYLSKEFSYRGQIFVWIIADFIKIVGL
                     CFVWIAASRSTNDISQSYVISYYLLLLLVSKLTTDFTPEYGLKNIASGVFSNYLLKPF
                     SYLTEYLGINLASNVLRFLIFLPAFVFGVIIASKFDLLIFGCNPYHIFLALVAIVLGF
                     LINFLLGNILTLISFKIKETDGIRIFYYNIAAFLSGEYVPFIFLPFVLQFGLQMLPFR
                     YTLSFPVEILTGRLGSYDLQLGFLIASIWLIVLWLAYKLIFKLSIKKYEAEGI"
     gene            29879..30679
                     /locus_tag="PKI16_00680"
     CDS             29879..30679
                     /locus_tag="PKI16_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNR52988.1"
                     /translation="MKLRVFSHYIKVWLVIVKASFRRSYVYRTEVLVRFVRTFFILGV
                     QILLLNAVFGDNEVYVGWTKNEAYLVMGIWNFLNYMGWSLFGVNLFNLENKVISGEFD
                     FYLLKPISSAWFASFCDFFIYNFVTVLSGLSLIGWYIYQEWSNLEYTNIILGVVAIFI
                     ALILWYGFYLLFASFTISNPRNGYLVVAKELLGITKYPIDIFGNTFQILFYTLLPIAF
                     LTTVPAKTFMGEGSYIVLLCGLLVGIATVIIGKNIWNRNIRNYISSGG"
     gene            complement(30663..31658)
                     /locus_tag="PKI16_00685"
     CDS             complement(30663..31658)
                     /locus_tag="PKI16_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNR52989.1"
                     /translation="MKSQTKEFKKRIKSAKSILILTHKGPDLDAFCSMLATYGFLKEH
                     FSKKEITLLARQLPTINMPMMKDINIVEKIEKQEADLVIVTDASNLSLCLEDGIDTFE
                     SSNIVYLDHHSVEVEAQEIFTINEYRSSATEQVYATFKDMFGKRLRVSKDVAELIQYG
                     IVSDTGRFLYSMTSSDTFRIFADVKDISPIDMEEFTQKNTKFPKETTPAIIKYLESLT
                     IEKDMAYMYIDREDVDEINIKQKGVNEAQSFLRDRYIRNIQGVHWGFIIKPDFDKEDS
                     WYVSFRSTKGYQDVSNIAEELGGGGHVYASAVPMTGKSAKEVLDKVLVAVSNHQS"
     gene            31732..33003
                     /gene="serS"
                     /locus_tag="PKI16_00690"
     CDS             31732..33003
                     /gene="serS"
                     /locus_tag="PKI16_00690"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNR52990.1"
                     /translation="MIDVKRIVEEREYVEKALLKRVAKENLDLDSIVELYNKRRDLQM
                     QFDTKRAQQNSFNEKMAKVEKGSDEFKSTVAELKEKSEEVKKIEESLRMTEKELKEKL
                     DVLPNIPEEDVVGGGKENNAVIKEVGKKPTFDFEIKDHVQLGKELGIFDFETASKIAG
                     NNFAMYRGLGARLEWALINFFISKHIEDGYEMVIPPNLATEESAYIAGQLPKFKEDVY
                     WVQDGLCLVPTAETVLTNIFKGDMLEEKDLPKKFFAYTPCYRREAGTYRANEKGLIRV
                     HQFNKVEMYHFTTEEKSEESFEEMVNKAEAIVTALGLHYRVVKLAAGDCAAGAAKTYD
                     IEVYIPSMDMYYEVSSVSNVKEYQSRRGNMRYKPDDGGKPKYMHTLNGSGLATSRLMV
                     ALVETYQNQDGSITIPEVLRPFMGNIDKISK"
     gene            33009..33584
                     /locus_tag="PKI16_00695"
     CDS             33009..33584
                     /locus_tag="PKI16_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNR52991.1"
                     /translation="MSFFFGFFPDENTNYKIRKVVGEVGRVFADFQIPVRWSKPETYH
                     MTVLHVGERLSIFKRLVWRFKLKTICIKPFKVSFNTVRLGISRKYKELVYLDTNEGGD
                     VMREMLLDMRKVLKLKDEGNFVPHLTLGRVSKDLSDEEYSNLCKDLAVVAKGLDIEKI
                     EFYVNDIKLIKSSGDNYSVLMNLVDLSKTKS"
     gene            complement(33536..34390)
                     /gene="miaA"
                     /locus_tag="PKI16_00700"
     CDS             complement(33536..34390)
                     /gene="miaA"
                     /locus_tag="PKI16_00700"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNR52992.1"
                     /translation="MVIVIAGPTASGKSKIALQLAKNINGVIINGDSRQIYKELRIGT
                     SRPIESEFEKISHYLYGHISVKEKYNIYKYQKEVKEILNNLPKGKIPIIVGGTGLYID
                     SVIYNYTLKEEDASLQREDLENKSIKELHSLIDSTILNQLNESDIVNPVRLRRIIEKG
                     ELGKQRGEKMNCLYLVMNVDKRHLEKNITERVTQMFDDGLLEENIYLKDNNLFNSTVS
                     KIIGYKEFTGYFDGTKTIEDVKSEIIKNTKRYAKRQKTWFKRNKDAIWVDNYDLVLER
                     STKFIKTE"
     gene            34432..34611
                     /locus_tag="PKI16_00705"
     CDS             34432..34611
                     /locus_tag="PKI16_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52993.1"
                     /translation="MVKIETTREDISRKRSVEDTKIVQRQNNVISTKESLNVSLRNSV
                     YSISNVKFLFLEFRL"
     gene            34693..34863
                     /locus_tag="PKI16_00710"
     CDS             34693..34863
                     /locus_tag="PKI16_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52994.1"
                     /translation="MSKTILRTMKERIVSNPDMARETIEKRVICLLLVKRYIQRGTIA
                     KQSNVITSVKSF"
     gene            complement(34907..36118)
                     /locus_tag="PKI16_00715"
     CDS             complement(34907..36118)
                     /locus_tag="PKI16_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52995.1"
                     /translation="MKLPKLKLNINIASLLNYIFVILISSVFVATILIALPLTEKYLN
                     NTPYNLNTKDSSYWSKEYLLTIDSKEQKDIQKTRDILFKRLQKFGVEQMSVAQKGNDI
                     MVTITTSKEKELTEQLIKNRFAVEIVTRKDDVNYEDPENPYAYLLATNYNSTDWNRDD
                     FRNVYITKLKNSAGTESYFAIFKLWPAKSVEFNKFLTQYEGQTVGVSTDGFVSPVQVP
                     AGPNKLFAPPVSTEDPQEINIIDILYNSGSIPANISLTSEKDMIPNIPQTNYIMVSVL
                     IFAGLLISYIYLFISKNASKPVLIKSFLATILTISLYLTILKFLQIPVDTFLLPIEAI
                     LTMVLIRVISENKDSVVYVDIILFGVLVVLTFLGFGYISIVARNMIALLAISKITLLL
                     SGWYINKVRKI"
     gene            complement(36120..36638)
                     /locus_tag="PKI16_00720"
     CDS             complement(36120..36638)
                     /locus_tag="PKI16_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52996.1"
                     /translation="MTNFNASFHVRGLVRILDEEINAVRRELLNLQDAGILKSHKEGN
                     KLVYKLNKDCSILWELRSMFFKESKIGTAILKETATVEGIQVIVLTDSFLKNEYNNPT
                     DLDLLVIGTMKIKDLSNAIAQVEKELGREIRFSAITKEDFDFARKKKEPFLMNILEND
                     KIILFGQISDLL"
     gene            complement(36714..37994)
                     /locus_tag="PKI16_00725"
     CDS             complement(36714..37994)
                     /locus_tag="PKI16_00725"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNR52997.1"
                     /translation="MPKEINISDNKGEIKPTETKAPAIAEQTKELEKKVLEKLPKKKK
                     IDRNILIFSIVAFLTLCGLGLFFYFGIYKVKPVSPQTVIPFAEDFVASIDSNSPFSRS
                     LTSLQKPSEPRTEVSPLNGVLFTKTEMDKLKKRRPVAVMSSNHADARPLSGLSSADIV
                     IEANAEGGITRHMAIYWSEGPEKVGSVRSVRQYYLEWASEYSPLFVRVGCASSTDPRV
                     NACSNIHYYKEIADIELFWRWDDGRRYAPHNAYTSVKGVWEKADKYKWNNFPTTIDEW
                     KFKRDAEIVDRGEKSVVKTVFHTSLPNGGQYDAIWTYDKGTNRYFRKVGGQADIDQET
                     NKQISAKNVVIQEVKMNYTGDSKSHIIITTIGEGNAKILQDGKIIDGKWKKKSRTDRT
                     MYYDSSGKEISFNRGMIWIAMVPQTYGKFDIIEQ"
     gene            38093..39073
                     /locus_tag="PKI16_00730"
     CDS             38093..39073
                     /locus_tag="PKI16_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR52998.1"
                     /translation="MKKIIFIIVILLLLGAGAYFIFKKPKVEDTNNTDTTVEEEQDTE
                     TKEEEKEELIIQDEEDFTGFSDDEQVIGVATEDLFNIESITDESKTGYHRFSFNLSTT
                     GTDDPYVTAKYNSSTGVIRVELNSIDSDKSGIGYQKDRAINKEGIVRLYHAVTSTADK
                     EIYDIGLAKSTIFKLYKQSGDGQLKVILDVKYPGEVTSTIDLGSTEFSTDLQDISGIA
                     NATIKSYSYSSSGGILKFVWSVTAEGENPIPSVTGEYLDDNTLSIKFTSLTLDRVVNA
                     ASSLTLPTGITIDSSKEGSASIYNFIVGSKKEYKLSATTSPNQVILEIDL"
     gene            39148..39558
                     /locus_tag="PKI16_00735"
     CDS             39148..39558
                     /locus_tag="PKI16_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNR52999.1"
                     /translation="MIRGIVCDFYNVLYNSSRKEINTEVLDILKECYSQNIPLFLFTN
                     SSLQFLHSENNKTTFLNIFSRYISMCEYNLVKPDDRAFEILIKEVGYDPIDVVLIDDK
                     EENIEKAKELGFKTIQYIDPVQLRKELNNLDIII"
     gene            39559..39632
                     /locus_tag="PKI16_00740"
     tRNA            39559..39632
                     /locus_tag="PKI16_00740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39593..39595,aa:Pro,seq:ggg)
     gene            complement(39634..40647)
                     /gene="prs"
                     /locus_tag="PKI16_00745"
     CDS             complement(39634..40647)
                     /gene="prs"
                     /locus_tag="PKI16_00745"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNR53000.1"
                     /translation="MTRQKAPLSIISCKSGLEFAKKVVKELRKISENKVHLIDSTEIL
                     FANTEIKTVLNESIRGKDVFIIQDVENHSEGMSVDDNLRALYTTVDACRRCDAERITT
                     VLPSYPYARQDKQDGREGITASRIAYELEGDGAVNHIITIDLHNSAIQGFFRRAKVDN
                     LKGSYVLLPYLKKLIKDTENSVVMPTDLGGAKRANYYAHQLKTDIAFCYKKRNYCKPN
                     SIEELAILGEVKDKDVYIIDDMICTGGTLVRAIEEAKEMGAKKIVAVCTLSLFNNPAR
                     ERFDSAFEKGILDLVIGTNATFQTKEFLKECRWYKQVSVAKYFAETIDRVNRRESITD
                     LLG"
     gene            40746..42557
                     /locus_tag="PKI16_00750"
     CDS             40746..42557
                     /locus_tag="PKI16_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR53001.1"
                     /translation="MEKKSNIPFKEYLKISLWSLKVTWGISPFAAIVILSTEIIRNLR
                     GIVNTYIIARLIDRLISLASNDGTKVLDILPFLVIILVVNILFTGIDELRNYASRIVR
                     RLSQPYLEKMSFEKVNFLGVQTLELPDVANAKQKMQDWLYSIMDVGNNVVRIIAAFVK
                     TLVAGVVLLKTIPLIIPLIFLTTVVFYFQRRYFFKKEFDWQTNDKHLIERRRSYWIAE
                     RLSDSTSIGEVSITGAYNYLSSKFTAFFKYYNEGLMKIMKKDAISSFLISILSNFVMI
                     VGYVQVFQLLLLKKITIGETTFYMSTISNFYDGIEWFFSEIVAYRDLVMKMKEVYEFF
                     GLKPAIEDGKEPLERFVTPPVIEVKNISFHYPNSKRNIFKNFSLKIEAGEKLAIVGEN
                     GAGKTTLVKLLSRIYDPQEGEILINGINLKDIKINDWYKNLGVLFQEYNFYGELTAEE
                     NIYMGKSMKDINRKKLIEASKNADAHDFIKKYENGYKTVMSERFRGGIRPSTGQRQKI
                     AIARFFYRNAPVAIFDEPTSAIDAESEYRIFNRIYNFFKNKSVLIISHRFSTVRNADR
                     IIVIHEGKIVEEGSHQELLKMNKRYANAFKKQAEGYL"
     gene            complement(42857..43450)
                     /locus_tag="PKI16_00755"
     CDS             complement(42857..43450)
                     /locus_tag="PKI16_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53002.1"
                     /translation="MSKVLGIIISVALIVGVGGYFLATKANAATPADSLFAVDTTFES
                     VQRLLTLDDVAKVNLEQAILEERQEEIETMLGDEDVTDEELGEAVRLMAQQRLRAYER
                     LGEVVQKQEEKGNTNAAEAIQTARERYLEHLDQQLETANKAQKKIKDVDKDITEEMEQ
                     EIEEEKNILKNTGENTQNQQQNEEKNTDNKDNGKGNN"
     gene            43590..43796
                     /locus_tag="PKI16_00760"
     CDS             43590..43796
                     /locus_tag="PKI16_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNR53003.1"
                     /translation="MKVSIDKSKCIMCGSCVAICPEVFEMKDDGSVDVKEQYKEKDIS
                     DDAIIAKVKEAEGACPATAIVVED"
     gene            43802..44218
                     /locus_tag="PKI16_00765"
     CDS             43802..44218
                     /locus_tag="PKI16_00765"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNR53004.1"
                     /translation="MDDCIFCKIIKGEIPSYKVYEDENVYAFLDIKPLSKGHTLVLPK
                     QHYKDIQDIPDELLCEVNKVSKRIVKKIQKKYSPLGFIINQNNGERAGQTIAHYHLHI
                     KPVYEGTVIPDEMNHRRELSKEEMNGVLKDLIEEEI"
     gene            complement(44198..45031)
                     /locus_tag="PKI16_00770"
     CDS             complement(44198..45031)
                     /locus_tag="PKI16_00770"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNR53005.1"
                     /translation="METTKSRISFSDIILFLIFLALIAIPGYLCYQIFFVNNKPEQQE
                     EVKEETPKTEVIEEEEEIPDGPHYEEKIFAISGQIAYIAIPTNIDENDPPRIVIYNHG
                     SNTRVTENLEDPFMKDLQIYGEEYTKSNLIFAASNAHDENWGNAASIQDNFNMVEWIR
                     NNYSTSRETFVIGFSMGGLPAMHYVAKYPKDISKVALLAPTTRSYEWNKSNIDVLDGI
                     DIQIWHGTKDVNIGITNSRSFVNSMDKLGKKITLIELDGKTHFDLDTEYRDEVLDFFL
                     N"
     gene            45273..46019
                     /locus_tag="PKI16_00775"
     CDS             45273..46019
                     /locus_tag="PKI16_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNR53006.1"
                     /translation="MPKTKNKTEGEIVINLNTFAIPIAIIVAGLLIAVGIFFANKGKD
                     TQQNIQGTETTTATEDKFTATSTTVDDDPYLGDKDKVKVAVVEFSDFQCSYCKRHSDE
                     VFPLIKSEYIDTGKIVYVFRDYQFFGGLSIDAAMGGQCLYDLTGKDNSKYAEYHKGIF
                     GLTSKDAITTLAKTLGVDESKFKACMDATKYSEEISKDQTDGTAAGVTGTPGFVVGVL
                     KADGTVEGKFVGGAYPIESFRTLIDEMLAK"
     gene            complement(46069..47217)
                     /locus_tag="PKI16_00780"
     CDS             complement(46069..47217)
                     /locus_tag="PKI16_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53007.1"
                     /translation="MNILKRVLPLLFVSVIFFLPYKVEAAQFFFKEQTVEQETTIEED
                     VYLLGSNSSVYGVIDGDLFVLSNKVVMEGTVTGDMYVVGSDVTIDGNIYGNLVVIAGE
                     SNISGVLSNNACVLSASLIYDGHAGKDLLAVSMNSDMKGTVGDDLRAFGYTNSIESVV
                     KGDALVLASQYRILKENVTGNIYNTNTLKDIAKEQGVDLKAEQTKRTEITRVSDYSLK
                     ILNAFLTFVGLSIAGYVLIFLSPVKTGQIVNKVSKSPTEFLKSLGVGIAILLLIPLPL
                     FLLAFTLVGIPFALLILGVLLFLTTFGTIYVESALGQEILELFGTKGYRPYKGLLTGR
                     VLTTLLSLVPVFGVFYSITISATAVGAVVRMKKDSFDNVKKINKKKKK"
     gene            47328..47588
                     /locus_tag="PKI16_00785"
     CDS             47328..47588
                     /locus_tag="PKI16_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53008.1"
                     /translation="MKKNKIYSTTEFYKNPSKILGYVKDGEVVYLGYKKLKDPIAVIT
                     PYKDYKPKKERISLWKLSSKKSVSAPEYADSLKFIQSQRKSR"
     gene            47585..47962
                     /locus_tag="PKI16_00790"
     CDS             47585..47962
                     /locus_tag="PKI16_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNR53009.1"
                     /translation="MNGYVLDTSFLSAFYNTKDVNHKEALEMSRNIDVGNLLIPIIVI
                     AELANFKKNEKFRRLLLKEAMNIASGIPSLDENDLEEYLSFIENLPNNMTAIDSMILF
                     LSVKYNAVLVSFDKKMMKIANNI"
     gene            48006..>48195
                     /locus_tag="PKI16_00795"
     CDS             48006..>48195
                     /locus_tag="PKI16_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53010.1"
                     /translation="MKKVLNIIQRYYPAEGGAERFFWTLSEYLANDLGIEVDVWTTDA
                     LNSETLWDLDGRKVEKKEE"
BASE COUNT        16390 a         7432 c         9136 g        15237 t
ORIGIN      
        1 acaattatta atgatgaaat tggcagctgg aaagtagaag atttaccaat atctaagaga
       61 tcaaagacag gacttttagc tggaaactac aaaactgtag cagatctaag gagtacaaca
      121 gcaacagatc ttttgaactt accaggattt ggaaacaaat ctcttaatga agttttagaa
      181 cttctaaatc aatatggtat taatataaga ggaatataag aatgtataaa ggagtaaaaa
      241 aatcaaaatt aggaagaaaa gcatcaaata ggaatttact cctgattaat ttgacaagat
      301 cacttttcaa gagtggccag attgttacat cttctgcaag agcaaaagct ctcaagcaga
      361 cagtagaatc tctaatctcc aaaatgaaaa caccaaaaaa cgatttggtg ctaagaagaa
      421 atttgcagaa gatttttggc aatgatgaat tagtaaaaat tgctatggaa tactctaaga
      481 aagaaaaaac tggagttgtt attagaaaag taggatttag aaaaggcgat aacacagaag
      541 aatcaagaat agaattaatt ggattagcaa agaaaaaagg taaagtagta gtaaagaaaa
      601 caaaagaaga tgagaagaaa gaaggaaagg aaacagaaat taacagagta gacttagaaa
      661 agagatccac acagaagagt atagacaaat ctgcagtgtt taaaaaagca gaacgagcta
      721 agagtagatc tggactttaa ttaagaaata tagaagatga agtacaaaac aacctggaca
      781 aaaaaagaag atgtaaaaag aaattggtac atagtagatg taaaagacca gatattgggt
      841 agagcagcta caaagattgc atctctctta atcgggaaag agaaagtaaa ctcagttcct
      901 aaccttgata acggagacta tgtaatcgtt attaatagtg atttcataaa actcacaaga
      961 ggaaaagaag agacaaaggt ttaccataca cattctacat atcctggtgg acacaaagaa
     1021 acctctttta gaaaaatgtt aaaagaaaat tcaccaaaga ttattgaaaa tgcagtattg
     1081 aatatgttgc caaataacaa gttgagagat acaagaatgc caaggcttta tgtataccct
     1141 caagctgaac ataaaaatgg aggccagaag ccaattgaga ttaaattata atactattaa
     1201 atagtggaga aatatttcga aagcatagga agaagaaaaa gatcaacagc aagagtaaga
     1261 atctttgaag gaactaaagc atctacagta aatggaaaag ctttggtaga ttttgtaaag
     1321 ggttctaaca aaacagactt gtatattgaa agaccattga tagtaactgg attagaaaag
     1381 aaatattatt tctctgctca tgttaatggc ggtggtataa caggacaagc agatgcaatt
     1441 agattaggtc ttgcaagagc actctatgat atgaatcctg atcttaagac aatcttaaga
     1501 aaagaagatc tcgttacaag agatagaaga gaagttgaaa gaaagaaata cagtcaactt
     1561 aaagcaagaa agaagcctca gttctcaaag agatagtttc tcattgacat catttcagta
     1621 tttgtataat atttatatac taagttctta tatttagtat atgaataaaa cattgaaaat
     1681 agctctaatc tctggagcaa gtttaattct cttgagtgca atttccattt ttgtattaat
     1741 tcctcttcta aaaggtatat atcaccaaat aaggtatata tcaccaaatg atgtaactgt
     1801 cgacggtggt ttaactcctg aagaacttcc taactcaagt gggaaagtaa cagattcagt
     1861 tggtcaagct gaaagtagag aatctgcact taaagttatc agagtaggaa atatttcaat
     1921 atccgttgat gatatagatg ccacaatagg gcaagtaaat actacacttg aaaaatataa
     1981 gggtgagact ttaggtatgc aggatagtgg caaagggaag gacagaattg ttagtttaac
     2041 tcttagagta aaagaagttg attttgatgc tttttaccaa gaattgaaag atttggatgg
     2101 agaatatgaa tattccacca tatcttcaag tgatgtaaca gagacggttc tagatatgga
     2161 agcaaggtta aaaaatttaa aaagtgtaga aacgcagtat cttgctattc tcaaaaaagc
     2221 aacaacagta agtgatactc ttgcagttca aagagaactt acaacagtaa gaagtgaaat
     2281 tgaatatctt gagagcagtt tggataatat ggaaagacaa actgactact caaccgtata
     2341 ccttacaatc actcaaagta gcacaggttc atcgttaaca gatgaagact ggagacctat
     2401 cgggattctt aaagatgctt caagagcctt agtaagcttc gcgaaattct taggtacggc
     2461 agttatatgg gtattagtct tttctcctgt agtagctgcg gtagtaattc cagtggtact
     2521 tattcagaaa aaggctaaaa aatagtataa tatcaacgcg caagtattcc tagatgattg
     2581 ctcttactaa ttttagtagg agaggaaagt ccggactcca tgtcggtctc gaacatattt
     2641 cgagataggt gccaacgtga gttggaaggg agtgatcttt tggagaaaag ggcaacagaa
     2701 agttataccg cctagtttac taggtaaggg tgaaatggtg gtgtaagaga ccaccgagta
     2761 tggtagcaat accatattgc gaggtaaccc ccatctggag caaggtaata ccgcttagat
     2821 agataatcgt cataaacaga attcggctta ttgggtactt gcgcacttca tattccaggg
     2881 cagccccaag gctgccctaa aatcagactt tcctaatgta ctacctcttt gcgttataca
     2941 catttaaaag gaagaatatt ttgaagaaat aacgggaatt ttaatttggg ggaattacac
     3001 caaattaaaa atgagaagtt gaatatgttt ctattgctga tttagcaaga tacgcaactc
     3061 ttaaggattt caaaggtcac gaatttacaa cctttgaaaa taatcctgat tttatattct
     3121 cactctaaaa cgattggaat ggaaaaattt aggggttggt acaagggcag ccctaatttg
     3181 gtcgaatttg accaaattaa aagggagtga tgacccattt acaggatata taaggaagga
     3241 gaattgaaaa gagaatcaat tatttccaaa atggaaacag ttcaagctgt tcggaatttc
     3301 cgaacagttg ccagagaggt gaagatttgc aaccaatgag catattctta atttgcttac
     3361 aagaaaaaaa cggcagaatc aggttctatg aagaaaatcg gtaatctcga tttttctact
     3421 aataaaccga ttaattattg cagtgtccaa tgaaaatggt tctgcgaatt tagggcaacc
     3481 ctagggctgc cctggaagtt aatcggaagt tcatattcgt ctgctattct tcatttatat
     3541 gaagaagaac agaaacaata caatatacaa gccgaattcc tattaccata tttacaatcg
     3601 gggaaaccac aagaagaata tattcctcca ggagaaggat tacaaagtct ttcgaggatt
     3661 gatgtacaaa tatcttagaa agtacaaaat tttattggtg gcgtattgct accttcccaa
     3721 ccattatcat tttgttttta aatgtgcaga ctattggcag gttattccga aattgatggg
     3781 tgcttttatg acatcctatg tcatgtattt taatcgaaaa tatagcaagg ttgggcatct
     3841 ctttcagggg ccttttggag tgagaaggat tgtaggaaac aaagacctac acggtgtcat
     3901 tgcctacttg cgaaggaatc cagttgaagc ggggcttgtt agtgaggaga acgtagatag
     3961 ttaccagtgg ttacatatta gaaagcagat tccttaacat tgggcggggc agccctaggg
     4021 cagccccgct tgatatttgg gatagggttg gtgtattgta ttagaagagt aatattagtc
     4081 tgccttaact atgaaaaaaa tgtcttctct gtttattaag ctcaagcaac ataagcttga
     4141 taatcatttt ttgtatctaa tcccagtgat tatagttacc ttgggtgtaa tcttttcagt
     4201 taggattttt gcagaacaga gtggtggttc tccagtatca aatgaaaatt ctcgtattgc
     4261 agatattaat acttcattaa ccacattggg ctatggttct acatctgcag gtacttgggg
     4321 agattggggt tctatgtgga atcgtatcag atctgcagca gagtgggctc ctacaggtac
     4381 tgctacggcc gctgatgtct tcccaggtaa aactttctat gccaatggta caagaagtat
     4441 actcacaggt tcagctgtgt taggtaagta tccagagcaa gccaatatct catacgatga
     4501 ctggattgca gccgatggta ctgcaaacga atacattgca gaggaacaaa cttggactct
     4561 aacagcacag ggtggaactg cagcttctgt tactgataat agtattacac aggcactctc
     4621 atctaacaaa gtctataagg atgctctaac caaactatat tggtcagata aaactgtcaa
     4681 tacattagat aatgagttta tatggactct aggtgataat cgagtcaatc caactaatac
     4741 ctcatgtaat ttcaactcta caggaaatgc taatgagtat tgtgataatc aggatccaac
     4801 aaatgcatat acagaggata atgatgtttc tgctacagag ttttgtctca atcttcagtt
     4861 agatggagat aatgcagatg cagataacaa tgggcttaca ggtgtagaaa cagattggag
     4921 gcttccctct cagaaagagc ttatgcaagc atatatagac ggtagtgcaa ataatttacc
     4981 caatgctggg aatgctttct ggtcctctac tgagcgctca aatattagag ataacgcgtg
     5041 gctcctctat ctaaataatg ggaccactta ttacactact aagtcaagta ctagctatgc
     5101 gcgttgtgtt cgagggggtc aataaaattt tcttaatttt tcttttcaat ttaaactata
     5161 gggtattgcc atatcttagt tctttggtat accataggat tatgcaagat ttcacactct
     5221 cgaccttgga taaaggctac ggcttgtgaa gaaaggcgaa tatttgaccc tacttgtcgt
     5281 acccaatggg tattgtgata caggagacct tctctatgcc taagctgagc ttaaggacgt
     5341 ccagatctcc ggtggatagt agaaatcgac aagttatctt tctggtcctc tactgagaac
     5401 tcaaataata gagctaacgc gtggaacgtc aatctaaata atgggaacac aaataacaat
     5461 actaagtcaa ataataacaa tgcgcgttgt gttcgagggg agcaagtatc atgcctttag
     5521 caaataatga aagcaatggg aaagtttaca ttgctttgta caaattaata aaatatctct
     5581 acggcctagt gcagaacttc ccaaaggagt acaaatatac actaggcgga gatattttaa
     5641 cattggcttg ggaaaccctc gactgggtaa ctacagctaa cggacatata aattcccaaa
     5701 aagcaggata tgtaagaagt gcattaatta gtttccaaaa actgaagtac agaatccgaa
     5761 tggctcacga gctgaagctc ataacagaca agaaaatggc ctatctgatt aaaacagatg
     5821 aagaaattat taaaatgcta aggggatggt accgctggac gcagggacaa gcctaaatca
     5881 gacttaatat tgaatgaaga taggtcatcc ttagggtcta aatactcctt taacatccct
     5941 agatggttat tgtaaatatc ctgtcgtaga ttaaaggagt ttgcccattt taaatgtcca
     6001 aatagggagt tgatgctagt acagattgtt gcaatctcgt cgtaagatgg tggattatga
     6061 agtggaacag cttcttcatt acagtaagtt ctgttaatta aaaatccaga attaaagtag
     6121 taaagctttt tcttcaaatt agatacgacc cgctttcttg aaagaacata atttggttta
     6181 acaaaataac caagaaaatc tataccatca tataccgaac ccaacttagt tttctcatct
     6241 tttagcttta atattaactt atcttctaga aaacgctgga ttttttcctt tgccataaaa
     6301 agttcatctt tgtcattaga gagtaaaatc atgtcatccg catatctgag gtaatacttt
     6361 atttttaatt ctcgcttaat aaactggtca acttcattaa ggtataaatt ggcaaaaaac
     6421 tgtgaggtaa gattcccaat aggaagaccc ttgttaggag gattagtaaa aagactctta
     6481 ccttttggaa gagtattaaa aagtttggga tcacctttaa taatataatg ctttgaagga
     6541 tcattaaaaa tgataacttg gcttagccac gaaatttcat taaattcatc ttcggaaagt
     6601 tttaatttct tgacatgatt aattaaaatg tcatatagaa gagttttatc aatattgtag
     6661 aaaaagctct ttatatctaa ttgcataaaa tatgctttag atgtctgatt tcttgttatt
     6721 ttgttcaagt atttacgaag ctttaacgct ccagctatac tgccatggtt tctcctacag
     6781 gcataggagt cattaataaa agttttatca attttaggct ctattttatt tattaaataa
     6841 tgatgaacta ctctatccat aaattccgca gcaaaaactt ctctcacagt tggatatgta
     6901 attacaaaac aagtagaata cccgggcttg taagtttcat tccctatacg cttttctaat
     6961 gaacataatc tagattccca attgaattca aaatcaaggg cataattaga atttcgttta
     7021 tttcccctac aagatctata tgcctcaatc aattcctcaa aagaatctgc catttaacca
     7081 atctataatt tattttccaa aagatatatg atatgttatt ctataagtga gggggtaagt
     7141 ccctttttaa agctatgaag atcactgctc acaaaataat aaaaaataca ttacttttag
     7201 tctctactct tgccctcttt acaataattg gttattttgc accaagaatt ttcgcagctg
     7261 ggcaaagcaa tgtcctagga gaagccacag taatgaatac aggaaataga atagatttta
     7321 attctacaac ttacgcttct aatgttgtga tatcagaccc tgatcctggt aataacaaca
     7381 gacgaaccat ttcaggctat gcatggtcca cagatatagg ttggattaaa tttacaagtg
     7441 gagaaaccgc aggtgtatat gtagcataca gtacagggct tgtatctggt tctgcttatg
     7501 ttgtaaatac tgggggaacg ctggattttg ataataatgg aagccatgta gttgtagata
     7561 cccaaacagg gatattctct ggttatgtat ggtctaatga tttgggctgg attgattttg
     7621 gtgatggtga tgtgtatgta aaagatagta agaaaccaga aaatgtttca aatgtagatg
     7681 cttttaacga tagctctaaa actattacta ttgaaaatac atcggcactt tacaattgga
     7741 atagtccata ttttgaatgg tctgtaccta tagaccctga tgatacaagt gaatatgcaa
     7801 gtggtattgc tggatatttc gtatattggg gaacagatga tactacagtt cctgaaactc
     7861 aaggaacttt ccaaactgca aataatttca catctccaac actcacaggt acccatacct
     7921 actatctacg aattcaggct gtagatgttc aagggaatat ctttactgga gatttagcct
     7981 cctatacttt gtttacttac cattttgatg gggacaaacc aacaaatgta agctatatct
     8041 ctatgcctag tagtagtttt gggaatatta gcgaaatgtt cttttcatgg ccaagtagtg
     8101 gctctgtagc aagttctgac aacagtgcag tactaggttg gcaatacagt ataaacgata
     8161 cgaatagttg gacaggatct acacatgagg atagattagg gtttgactat attccattta
     8221 ttgattctga ctacacatac tattttgatg cagacaaaga tggccctaaa ataatagtgg
     8281 gaaataacat tatatatttc cgaacagtag ataccgctgg caatgtaagt gtgtatgtaa
     8341 gtggtggaat atcctatgga ggacaagcac ctacatttga agcagaagct caggttacag
     8401 taacacctaa ttctagtgat tcgaatagtt ttagtctgtc atggccagta gcactagctg
     8461 gagaggaaag aactgttgca aaatattact atatggtaaa tacaaatcca ccaagtagtt
     8521 atgcgacatt ggcaaataac agtagcttgt atatacctgt cgatggaaca tcagtaagta
     8581 caagaatgct aaaaggggct ataaagggat caaataccgt ttatgttgta gcagttgatg
     8641 atcaagatgg ctattcctct agcaatgtga ttacagggac atttgtttta aatagctcgc
     8701 ttccagatcc agtcttaaat ctatcattag tggatagctc tataaaagag gctgaacttt
     8761 ggagagcatc tttaagctgg ggtgaacctg tatataaagg ggatggtaat ttatcttatc
     8821 atgtacttcg aagtttaaat gggcagaatt gggctgagat aggtactacc acaggtctct
     8881 cctatactga cattgtttcg gagagtgcaa cctactacta taaggtttta gtaacagatg
     8941 gaactgatga aagtaaggct aatccaacgc cttcagcttc atattctgta aatctgaaag
     9001 gacaatatac atctccagca ctgttagtat ctggaattgt attaacagag gtagctatta
     9061 gacatgctac agttaattgg ataacagata gggcttcaga tacaaagatt atgtatggta
     9121 ctacatcagg gcaatatttt gatgaagaat tgtataattc tgtgcaaacg acagatcaca
     9181 agataaggct caatagtcta caagcagata caatttatta ctttaaagct aaatggactg
     9241 atgaggatgg aaatactggt atgagtcaag aaatatcttt tagaactgat cctatgccag
     9301 aagtgataac ttcaaaagtg gagagagtag gtttagattt tgcaacaatt tctttcgagg
     9361 tgaaaggagc aagtaaagct tctgtatatt acggtaaggg ctacacatac acggggttaa
     9421 aggagattaa tacctcacca gttgatagta aatataccgt aattcttgat gaattaagtg
     9481 atggtacgac ctacaactat aaaattagct taacagatcc agagggctat tcttacgatt
     9541 caatcgagac ccactcattt actacacctc ctatgcctag aattagcaat gttacaatac
     9601 aagagcttaa ggatgtagca agtccaacaa ttgttgtaag ttggtttact aatacagaga
     9661 catcttcgat agttacctat actgcagaag aaggaaagac ggcatccgtc gataaattgg
     9721 atacgaagat gaaagctggc tctcatagca tgagtatttc tggattgaat ccaatgactc
     9781 gatatgtagc aactgtagaa ggaattgata aattagggaa taaagctacc tctgatgaga
     9841 taagatttac tacagctact gatacaagac cgccaaagat ttcaaatatt aaagtacagg
     9901 gagacctgct aagcaggagc atacaaagtg agagaagtag aagtgctcaa ctcattgtga
     9961 gttgggaaac tgatgagcca agtacaagta gagttgaata tggagagggt acaagtggta
    10021 tttacaatta tagtacgcaa acagatagtg agctaagaac aaagcacgtc gttgttatat
    10081 ctgggctaac tccatcaaaa gtatatagta ttggggttgt gtctgtagat actgccgaaa
    10141 atgaggcaaa ctctacccca attgtaacca ttactccgaa agcctcaaat actgttttgg
    10201 aaacagttct taacagtttg ggaaatatat ttagtttttt ccagtagaat agaaaatgaa
    10261 aagcttagat gaagagaaaa ggaaactgat agatataaaa gaaattgaaa aaagtttcca
    10321 aactggagat aggtttacgc atgttttaaa aaatatcagt tttgaagtat atgaaaatga
    10381 atttttagtg atttttggtc cctcaggctg tggaaaatca accattttac atattttgat
    10441 gggattggaa aagccagatg caggtattgt acgatttgga ggagtagata tgtggaaaat
    10501 gaactcggat gatagagcag atgttcgaaa aagagatatg ggtattattt accagaaaca
    10561 aaattgggtt agatcactga cagtagttga aaatatcgca atatcagccc aactattagg
    10621 acttccaaaa gaagaggcta tatataaggc tgaagaaatg ctgaaacggg ttggtatatt
    10681 ggataaatct gattatatgc ctaccgagct atccgcagga gaacaacaaa aaatgggctt
    10741 agcaagaagt ttagtaactg atccaaaagt aattatagct gatgaaccta caggaaatct
    10801 agatgtaaaa agcggtcagg aaatgataga tttactcaag gatttaacgc aaaatggaat
    10861 taccgttatt atggttacac acaatcctga ataccttcgt gaatcggaca gaacgatatt
    10921 tatgttggat ggtgaattat acaaagaaat tattactaaa caatctgata tggatagcgt
    10981 tttgaaagct atacaaaccg atcttaatga tttcatacaa gggaagggga aaaggaaaca
    11041 attagagaat gatagaagct ttccaattac taagtttgta gaaaacgacg tgcctcaaaa
    11101 gaattttttc caaagtataa gctatttttt gaaatttatc ttttatttct tgattaatac
    11161 ctttacattg gcaataggat ttttaattgg aaagatttct gttgacaaaa tgaaaaaatt
    11221 caaagacagg atgactaaag tatctttttc aagagaaaat gatacccata tttcagaaag
    11281 tataaatgta ttagatttaa cggaaatatc atttcaaaat cttactacga ataaatctcg
    11341 tacatctact actattttag gagtatcgat aggcattggg attattgtct ttctattatc
    11401 cataggttat ggactagaga atttggttat taatgaagtt acaaaaattc aaaatcagaa
    11461 tgcagttgaa gtttcgtcga ttgttggtag caatgtagaa ttaaacgatg agttattaga
    11521 ttctattaat caaatttcgg ggataaaaga gagcttcccg ttaataaact ccgcggcgaa
    11581 atcttcattt aatggtgcta atacggacct agttgtttat ggagtgccag ttggatattt
    11641 agaacaatct caaaatattt ggttagcagg agatagctta aatgatagct ataatgaaat
    11701 tgttataaat aaagaatatt tatctatttt aggcggtagt gctaatgata tattggggaa
    11761 aacagttgat ttattaatga ttaatcagag cgtagaaatc aaagaagatt cggctgatgc
    11821 aagtaagccc tataagatag taggagtaat cgatgatgga aacccgtcag tggtatatat
    11881 gaatataaat gaaatgaaaa actatgttcc ggagggttat tctcaactaa ctgtaattat
    11941 ttcggataga acaaaactct ctgagattcg taaacaaatt gaggttttgg gctttcaaac
    12001 cagttctgtt atggatactg tctctcaagt acaatctttg tttaaaaata ttcgcttagg
    12061 gttggtgtct ttaggtataa ttgctttcac tatagccatt ataggtatgg tcaatacatt
    12121 aactgtgtca ttgttagaaa gaacaagaga gattggttta ttgaaaacga taggaatgaa
    12181 gtccgacgaa atacgaacac ttttccttaa cgaatcaatg tttatgggct tagtgggagg
    12241 catagggggt gtgatattgg gcttgatttt aggaactctt gtttctattg tgatatcaat
    12301 attttctttt acgaaaggtg gggaatttat tatgataaat gttctgccat ggtatatgtt
    12361 tatcatattg ccactagtga gtagtatagt aggatatcta acagggttat acccatctaa
    12421 ccgagcggta aagataccac cactggatgc gataagatat gaataaggca tgaactgagg
    12481 gcagccctgg actggggctt aaatatctct accaattcca tttaaaaact ctctttcgct
    12541 tgttttactc cttccttcta tctgtaattc aaatgccctg agagatataa gtggatcttt
    12601 agtagcaaat aaaacttttc catcatatga gaatattgct ccaggatctt tttcagaagg
    12661 tatttcaact aattccgctt taaaaatttt cataatcttc ccagctatat ttttattcaa
    12721 gcttccagaa agtctagtcc aagcaattgg ccatggaata agtgcacgaa ccattcgttc
    12781 gatatattca ggagtcatac tcttccaatt tatctgtgca ttctcttttg aaatatcctt
    12841 ttgccaacag tatgtcgcct cctctggatt ctgctttgta ggagtaatct ctcccattat
    12901 ccattcttca agtacatctc ccaaaacctc agaacttttt ttcactaacc tttctctaag
    12961 tgacaagttt gtatctgtat ctaaaatatc ctcttcataa atctttagaa tatctccctc
    13021 atctaaaccc tctgacatca acattatgga tattcctgtc ttcttctctc catctaaaat
    13081 tgctttttgt atagggacag cgcctcgata tttaggaagg atggaaaaat gtacattaat
    13141 acatttgtat ctaggatatt ccaaaaattc ttttggaata atttcaccaa atgctttgca
    13201 tacaactaac tctggtttga acttgtctaa agccttttga tacagctctg gtttcttgtc
    13261 tgtgtggaat actggaattc tattctttaa agaatattct ttaacttctg atggagtaga
    13321 tatctgtttt cgtccaacca acttgtctgg tgtagttatg gttccaacaa tatcgaacct
    13381 aggatcttca tgaagagcct ttaaagttgc tacagattcc catcctgttc ctagaaataa
    13441 tgtccttaca ggttctttaa ttaaatctct catagaagta attttactcc ataaagatat
    13501 aaattggtat aatacaaacc atgaaaatgt cagaatttaa atacaattta ccacaagaga
    13561 aaattgcaaa atttcctcca aaggaaagag gaacaactaa gctgctggtt ttagatagaa
    13621 agagtgaggc tatctcacat aagaactact cagatattgt tgaatatatt aaacaaggag
    13681 atgtaattgt attgaacgaa actaaggtag aaaaaagaag gacattcttt caaacagaaa
    13741 aaggaagaac attagaagtg ctgtttctta accatacaga ggatggaaat tgggattgcc
    13801 tcataaaagg tggaaagtat gtaacggatg gagatatcct cagggatggg gaaatagaga
    13861 tacaagttgt tggaagaaaa gataatggct ttttagtaag accattaaat atggaggcag
    13921 aagatatctt tgagaaggta ggtcatacac caataccacc atatatgaaa agggaggata
    13981 atcctgacga ttatatccgt tacaatactg tttttgcacg cttggagggc tctgtagcag
    14041 ctcctaccgc tagtttgaac cttactagtg aaatactggc tcagatacag aaaaaaggtg
    14101 ctaaaacagc taaaattgaa ttagaagtag gttggggtac ctttgctcct gtaagagaag
    14161 agaacattga agatcataaa atacatagtg aaaagatatc tgtcggaaaa aatactgtaa
    14221 agttggtaaa tgatgcaaga aaaaatggca aagatatatg ggcttttggt acaacagtag
    14281 caagaacact tgagagtgtc tctgatgaca atggattcat acatgaattt aaaggaaata
    14341 cagatttgta tatatatcct ggatacaagt ggaaatgtgt agatcattta ataactaact
    14401 tccatatgcc agattcaagc ttaatgcttt tagtatcttc tttcgcaggt aaagaattaa
    14461 ttaagaaagc ataccgagaa gcattgaata tggattacaa atttttaagc tatggtgata
    14521 gtatgttagt aatttaaaaa tatggaatta aaagattttg gaaaacaata tatatttctt
    14581 ccagatgcaa ccaggggtgc tgttagattt ctaactactg aacaattaaa agctactggg
    14641 actactgcaa ttgttacaaa tacgttacat ttgctcatat ctcccggtgc agaaaggatg
    14701 caagagttag gtggtataaa gaaattgatg aattgggatg gtattgtttt aacagattcg
    14761 ggaggttttc aagtattttc tctaatccat tctaagaaat ggagtggtaa ggtccatgag
    14821 aatggtgcta ctttcaaatc tcctcttact ggtgatgtgt atgagctaac tccagagagt
    14881 tcgatagata tacagatgaa gataggttca gatattttgg ttacattgga cgattgtaga
    14941 aaaacaaatt taacaagaga ggaagctttg gaatctgtgg agagaacaat tgaatgggct
    15001 agaaggtgca agaatcattt tgaaaaggaa tatggtggga caaaggagac tggtaaatta
    15061 ctaagctgtg tagttcaggg agctaattat ccagatttaa gaaagagatg tgcacaggag
    15121 ctttcagaga tggatttcga tggttacaat tttggagggt tcgttgtaaa taatgaaggg
    15181 cagttagtgt tagacgagat gaaagtggtt gtagataata ctccaaagga caagtttaag
    15241 tatgcaatgg gggtaggtaa gccacaggat attcgtaatg cagcagagat tggttacaag
    15301 gtttttgata ctgtcctagt aactaggaat gcaaggcatg ggactttgta ctcttcagat
    15361 attgaggggg aggttttgag aattagtaat gcagagtatg ctacagacca aagacctatt
    15421 gattctaaat gtgattgtta tacttgtaag aaccatagcc gagcatatgt acaccaaatg
    15481 ttaaaggttg gggaagctac gggtatgaca ttagctacaa tacataacct tcggtattac
    15541 caaaggctaa ttgagaccct gtcccagccc tgtccctaat atttgctcca aggataatct
    15601 tctggttttc taacaaggcc ctctcttacg gggttctgtt tgatatattc tttcgcatat
    15661 cgaagatctt ttttccagaa aagcaacttt gcgttgtatc ttccttggaa gacatgccca
    15721 attcgttgat attttcggtt aatttgcatc gaaaagcttg tgacaacttt acacatatat
    15781 gtagaaatgt tttgagggtt ttttcttgtt ttaactatca gatgataatg attatccata
    15841 atacaaaaag cgatgagtct gagatcaact tcttttaaaa atttgtataa caggtttata
    15901 aagagttgct tatccgcaga atttttgatg atcatttctt tattattgca gcgattgtat
    15961 atatgataat acgaatattt tttaaaattt cttcttcctc ttggcatatt tgattttctc
    16021 aaaagattgt gaaaagggga ttaacgaaaa aatctcaggg gacagtcttg ggacagacct
    16081 tgggaagggg gtcgttgagg tattaaacaa aaaatgatat cattagaaag tccttgtatt
    16141 taaacacttt taagtaaaga ttgtttttga tatagaaatt aatatttgaa tgaaaatata
    16201 tttaattgaa aataaaatat attcattaat tagcagcact caggctgcga atttccaaaa
    16261 aagggtttaa acaaaggaca agttaaattg ttattttgag ataatggata cattattagg
    16321 tattcttgca actgttttag ttacaggtgc aggatctgta gcattattga aatatgtttt
    16381 taaatgggga gatgtaagaa aaattcctga aaaagaaatt gaaaatgctg caaagaaaat
    16441 gatgagcaag aaatttgaag aagcggagga tgaaattgca gaactaaggg aaagggaaga
    16501 aaagagattg agagagatta gaaaagagag tacagagcat gaagagattt taattgagag
    16561 agaaaaagct ttaaacaaga gatccaagat tttggatgta aagagcgagg agctaatgca
    16621 gatagagtct caattaaaag agggaaacaa gagattagaa aattctagag cacaattgaa
    16681 aacagaatta gaaaagattt ctggtttgtc tagagatgaa gcaaaagcaa agttgatgaa
    16741 agaaatagag gaagatctta aaggttatga agccaaaaag attagacaag cagaaaagaa
    16801 tatagaagca attgctgacg aaagtgcaca aaagatactt gtagaatcaa tgcagagagt
    16861 tgcaacagac tatgtaggtg agacaacaac aacatccata aaagttgaag atgaaaaagt
    16921 taaaggtaga gttattggaa aggaaggaag aaatatcaga gcatttgaac agatgactgg
    16981 tgtagatgtc atagttgatg aatctccaaa tacaattgct ttgtcatctt ttgatcctct
    17041 tcgaagagag atagcatatg ttgctcttaa gaaattaata ggtgatggaa gaattcaccc
    17101 aggggcaatt gaagaatctg taagaaaagc aaagaatgaa atatcgctag aaattaagaa
    17161 gaatggtcag atacttgcag aggaagcagg ttggccagat attgatgtag ggcttttgaa
    17221 attgattggt aagatgaaat acagaaccag ttatggtcag tctcttatgt cacacactat
    17281 tgaagttatt agaattgcag aagtacttgc tgcagagctt ggagcagatg ttgatctagt
    17341 aaagaaggca gcacttctac acgatgtagg taaagtatta acacatagaa tagatagtcc
    17401 tcatcaccat atctctggcc agattgctag aaaatattgc ttagatgaga aacttgttaa
    17461 tgcaattgag tcacatcaca tggacattga accgacttca acagaagcag ttatcatata
    17521 tcttgcagac gcaatatctg gagctagacc gggcgcaaga aaagatagtt atgaacaata
    17581 tattcaaaga attgaaggaa ttgagaatgc tgcaaaagag gttggtggag acaaagtaga
    17641 agaggtcttt gctattagag cgggaagaga attaagagtc gttgtaaaac caactcttgt
    17701 ttcagatgat gaggcaatag tcatggcaaa gaaaatagca caaagtattg agaagacaca
    17761 gagctatcca ggcaatgtgg aagtatcagt aattaggcaa acaagagctg ttgaggtcgc
    17821 taaataagga tttcttaact atcttctagt aagaattgct atagggaaag gctttctaag
    17881 cctttccttt ttcttaaagc atactatgat gcaaagtgtt ggtatctagg cgtttccctg
    17941 cataatgatt aaaaaatgta agtattttca taccaaagtt tgtatacaca caccctcaca
    18001 acctatagta acaacagtgc ttgacgattg cttgtattta caatatttta gaagtggata
    18061 tacagaaaag gtttcaacga agccactatg tatatatcac ttaagaaagg aggcatacta
    18121 caatgttaaa aaaagattta atcgataaaa tcgcagcaac aggtttaacc aagagacaag
    18181 caggtgaagc tttggaagct gtattagaag caatctctgg atctcttaaa catggagatc
    18241 ctgtacttct aacaggattt ggtaaatttg aagtaagaac aagagcagct agaacaggaa
    18301 tcaatcctaa gactcttgct aagattcaat taccagcaac aaaagttcca gcattcaaac
    18361 ctggaaaagc attgaaatta gctgttaagt aaagtactgc cggcaattct ttgctgataa
    18421 gcgaataaaa ggggtcccaa aaggatccct tttgtttact aatttagata aatactttaa
    18481 actagaaaaa gaatatattt gatactgtcc ataaatgatt aaagaaattt ttattccaaa
    18541 caagaagaac aaatacaaac cataccttct taaaaaagtt tctttaacga tattctcaat
    18601 catcttaata cttgtaaata gcttcggtgg aatcttagga atagaccaag cacaagcaag
    18661 tacaataact cctacaaata tcatcaatct tactaatcgt gagagaactg ctgcaggact
    18721 aaatagctta aaaacaaatt cgaaactatc ttctgcagca ctagctaaag caaacaacat
    18781 gtttgaagaa caatattggg atcactttgg accaaatgga gaaactccgt ggcaattcat
    18841 acgaggtgca ggatattact atgtgtatgc tggtgagaac ttagcaaaag gattctctac
    18901 tgcagagggt gtgcatgagg catggatggc tagtacaagt catagagaaa atatcatgag
    18961 tggaaagtac aaggatattg gagttgcagt tgtagagggt gttcttcttg gaaagagaac
    19021 tacgcttgtt gtccagatgt ttggaaatct tacacaggaa gttgctggag taacaaaaac
    19081 tgtaacacca aagccatcta caaatactga aagcggagaa atcaagagta tatccatcac
    19141 atccccaaag ggtggttcta taatagttga tccatcagtt aatgtaaaag gttctgttaa
    19201 aaatataact ggagagtatt ctgtctcagt atatgaagga gaaaatttag ttggggaaac
    19261 tgagtccgag acaagtaatt gggagtttga caaggatagc gattggtcag agggagtaca
    19321 taaagtaaaa gctgcagtcg atgatttgaa aattacatct aaagaagttt cttttacgat
    19381 tgattccacc ccaccgaaag taacagagaa cagtatcgtg gttagtgaga acaatggatc
    19441 atttgtatta accctggaga tatctccaga ttggaaagat attcaggtaa taagtggaga
    19501 gaatacaatt agtttaacaa atagtggtga gaagaagata gagattacaa tccccaaaga
    19561 gagtataagt gatggtgttg tcctgatggc gtcagatgtt aatggaaaca ttacccaaat
    19621 ggatatttca gaatattttg aagaggaaga agataaaact ttcttacaag gattatctct
    19681 tctctcggtt gatacaagtg atggtataag tattgtgatt gtttcattta tgtttgtact
    19741 tctgatgatt gaaatatttg tatattggag gaaaggaaag ataagggagg cagctggaga
    19801 cctatttaca gttggtgttt ggtggcttgt aatagcggtt gcactcttca atggttttac
    19861 aggtataatc tactaatatt taaaactgcc aagtggaaaa actaaagaca aaagtacaaa
    19921 atgatatact atatctcttt ctcatactgc ttctaacaga gatattcaat tctgttgtga
    19981 tgagtggaga caaaagtgta cttctcacgg ttggtatctt gggtttgctt cttcttgaaa
    20041 cttggtttat tagagcattt agtatttact ccggtaagaa aatatctaaa tacagtgatg
    20101 taatagtaag aatatcttta aaagagagat tctttggata ttttgtatta ccagcaatat
    20161 tctatatctc gttgttagca tttttgttct ttaacaggaa tgtagtttta ggacattttg
    20221 tattgggagt atctatggtc ttgtttctaa tccttttttt gaatgtaaag agttctttaa
    20281 acaagatata cagattagaa aatgttacaa agatagtttt tgattttcta tgtatatcta
    20341 ttctgtattt attaataaat atatttgtaa ggataggaat gagtccagta tattttctct
    20401 tgagtgttgg agtatcttct tttaccctac ttttattcat tctaaagcta catgataaaa
    20461 tgggcttctt agaaatagta atatctttct taagcgcgat atttataaca tgtgtaacac
    20521 agttggcttg gaacttgagt atattcacta ttccagccgt aggtacactt gctttctatt
    20581 taataatttc aatatggaat atacgctttt cggggaaggt taaaatctct gactatatct
    20641 tgccatttat ctatgtaatt gttaccttaa ctttaatttt gaatctgtaa tatggaagag
    20701 aactttgttc taaagattgg tggttctgta atgtatgaca ataccttgaa tgttaataca
    20761 gatttgatga acaaggtgaa ggattggtat gtgaatgaga gaaggaatta caaaaaagta
    20821 gccatggttg taggaggagg ttctctctct agagatatgc aggaaaagat ttctagtact
    20881 gtaggtggag aggaatcatt acacagtatt gcaatgtccg ttacccaaac taatgcagca
    20941 gttcttcagg gatatcttgg agatatggat atctttcttc caaaaacatt gggggatgca
    21001 tatgaatttt taatggaaga aaaccaaaag actctgttaa gtggtggctt aaagataggt
    21061 tggagcacag atatggatgc agcaatatat gcagatatat tgaatgttaa aacggtatac
    21121 aagatatcaa atatagaata tctgtatgag gtagatccta gaatagattc cactgctaaa
    21181 ccaatattag atatatcatg ggaagaatat ttcaaattat tcaacattgt agatggagat
    21241 aatcatgaag cgaacaaaag tattccaata gatgtaaact gtgctcaatt ctgtgctaag
    21301 aaagggatat cattcttcat atgtggaggt agatatctaa aggagaaaaa cagaatacag
    21361 gaaatactaa aaaacggaac attgatacat ccttaagata tattctcgat ggtgtaatcc
    21421 tttttacctt ttggggtttc aattgttatt ttactcttta tcttcttatc cataactgca
    21481 attccaattg gagagagata ggatattttt ccttctagtg gatttgcctc gtcttcgcta
    21541 gttaaagtat atttcaatgt agtatctccc tttaaagtaa cagtgtctcc aaggtctact
    21601 ttctttttgt cttgtgtttt aacaacgacg gcatttttta atttttcttt aatctctaaa
    21661 atcttttctt cattgatatg gaattcctcg atggccatag agtatccatc attctcgcta
    21721 agatcccctt gatttctcat ttcattcagg gtgtttgtaa tctttttctt tagttctttt
    21781 tctctgtatg tgagttcctc ttttagtttt aggagtccct cttttgttgt tttgtaacct
    21841 tcttccatat ttatggagat atatttttaa tgggagtaat aatattccca gaatgcttat
    21901 ttgtcaaata tatcttgccc tgtagttact gtttaaaatg ggtatgggat atgataaaat
    21961 tagcgctgtc agatatgccg ctatcgtcta acggttagga cacaggattc tcaatcctgc
    22021 aatcggggtc cgattccccg tagcggtacc ataatatttt aatataaaat tcttcatatg
    22081 gaaaaaaatc cagtttataa cgaagccgat ctgaaagatc atttaggaat atggttatgg
    22141 ttggaaaatg aaaaaggcga gatattaatg ttttatcata agaaatttgg ttgctggacg
    22201 attcctttgg aaaaatctga tccaggcgaa acattgaatg aggctatact aagaacagga
    22261 actgaagaac ttggtataga tatcgcaaat tatgagatag ttcatacaca agggaatagc
    22321 tatattagag atggaaatag ggttaatgtt gaagcctctc ttgtaaaagt tctatgctat
    22381 gataacgaaa ttacaaatat ggaaccggat aaacatttag aagtagattg gaaaagtaaa
    22441 gagtttatct tgaaattgga agattctaca gatgcagtga aagcacttca ggaatatatt
    22501 aaatgaatat tacaatctat gaaattcgac atagtaacaa tattcccaga atcaataaaa
    22561 gaatatatta atacaagcat acttaaaatt gctcaagaaa aaggtttggt agaaatcaat
    22621 atacacaatc ttagagattg ggcaaaagac aaacaccata caactgatga cactcccttt
    22681 ggaggaggtc ctggtatggt aatgaaggta gagcccgtat tcgaagcaat taaagattta
    22741 aagaaggaag gaagtatagt tgcaattaca acaccaaaag gagagaaatt agaacagagt
    22801 aaattggtag aattttctaa aaatactaat ttacatatga ttattctctg tggaagatat
    22861 gaaggatttg atcaaagaat ccatgacaat cttgtagatt atgaattctc cataggagac
    22921 tatgttcttt ctggtggaga acttcctgct cttgtgcttg tagatggaat tactagacta
    22981 ataccaggtg tccttggaaa tgaagaatct ttaacagaag agtcctttaa tgatggaaat
    23041 ttagattttt ctcaatatac aaaaccagca gagtttaatg ggtggaaagt tccagatatt
    23101 cttctttctg gagatcataa aaaaatatcc aagtggagaa agagtaaaag tgaggaatta
    23161 acaaaagaaa aaagacctga tttgaagtaa aaagagagtt gtatataata ttaataatta
    23221 aattgttttt aataatcata tggaagaaca agtctcgccc aatatggtag ataagaaaaa
    23281 attgagagta aattttctca cagtttttct tattctagtg ataataggtt tagtagttac
    23341 tgtattaggt atgagtagtg ggtggaaatt taaggagttt gatttgaaga gtacatttat
    23401 agccaaggaa gattcaataa agcaagatgt atcaggaaga actagtagat ttgtttcaaa
    23461 gtatgggttt tcgatagatg ttcctaatta tagtgattat ggactcaatg aatttacagc
    23521 agaagagatt gagattttgc ataataaata tgaggtagcg acagatttaa aaaatcattg
    23581 gtatttagcg cttgcagata tagatacggc ggatgcttca agcaatcttg gaacaatcgt
    23641 agaagatgtt tcgttgacct ataacctttc gccagaggct tggaatagtc ttcaatctga
    23701 gaacgaagaa attagtcctt tagagattct gtattatcga gttcaaatct atgtgtacga
    23761 gaacacaaag ggattgacct tggaaaaggt tttgagtgaa attagggaga ctaatgacat
    23821 atcgggtgct gtatatgggg aagtaacata ttctgaaatc tctggggaaa aagtagcgaa
    23881 atttacagga ggaaattctg aactatttca ggacaatatc gtagctattt ctggagatta
    23941 tatttatgtt atagagaagg ttgtctctga taagacagct tctgaatcaa tgagtgtttt
    24001 ggatagtatc attgattcta taaaatttga gtaagtaaaa ttagtccatt agtactatgg
    24061 aagagaaaac tataatagaa gaaactaaaa aagtttctaa aggtggagtt ggcattctaa
    24121 gtatattctt ctttttgtta gctcttggat tagcatttgt aatctttggt tacaatacca
    24181 aatggaccta cagggatttc aactttaacg acatatttgc tcctgcagtt gtagaggagg
    24241 ccaaggaaga agaaactagc ggtgtaaaga atgctgggtg ggcattgttt actttgccac
    24301 aatacaaatt tagtgttgag ataccagatt acaaacttaa acagaaattt gatggacagg
    24361 atgtatactc atactgggat gtaacgcatg cagaagaata ttacgaacaa tctctttatt
    24421 atgataaagc attgtatgaa gatagtgtcg agattatgtt ttaccctaag aacttaccag
    24481 attccgtagc ttgtggtcag ggatgtgtaa acgagcatta tataaatatt gatatttaca
    24541 gaaatgaagg tggaaaagat ttgccatcag tccagaaaat aatagaaaca aatttagata
    24601 aagcttttga ttttggtggt gaaatgggtg caacatacaa tgggaaactt gttacaaagt
    24661 gggataggca ggtgtggagt ttctctatgg aatttgttgg aggttcaagc gatggctata
    24721 ttgttgttac aaatgatttt atttattaca tagaatatta cttctcagat ttacctactg
    24781 aatctaagga tatagctgat aaagtgcttg atagcattgc cttttttgag taattaaaaa
    24841 ttattgatta gtatatacaa ataacatata ctatattccg ttagttagat aatttctaac
    24901 aaatatacta atgaaagata aagtgttgtt aacagaagaa aagaaagaag aacttgagaa
    24961 agagcttaag cacttggagg gaaagaaaaa gaaagaggtt atggagagcc tagagcaggc
    25021 aagaagggaa gacgtaagtg aggacaatga gagtctaagt ttggtcttac agattaaagc
    25081 agaacttgag agaaaagtgg aggatattaa atcattgctt agtaattcta aaataattaa
    25141 aaagcaagct tgtgatccta caaaaattaa tatcggatct gaaattaagg taaatttcaa
    25201 agggaagaca actaaaatga aaattgtttc ctctgtcgag gcagatccct tgaagaatta
    25261 tatctcaaac gaatctccat taggaaaggc attaatgaaa gccaaattag gagatactgt
    25321 taaactaaag gttgacggta atacattaga gtataaagtt ttagaagttt gttaattaag
    25381 agcctcatct aaatcttcaa tcttgactct cttttgttcc attgtatctc tgtctcttac
    25441 tgttacagta ttgttttcta atgtttcgta atctacagtt atacagaatg gtgttcctat
    25501 ttcgtcctga tatctatatc tctttccaat atttcctcta gcatcccaaa ttactttgtt
    25561 atcgtttgaa agatattcaa atatctctct agctttttgc attatctcag gtttatttga
    25621 taccaatggg aagactgcag ccttgtatgc agctatatcc ttatgtattt ttaatactgt
    25681 tcttatctct ccatttggta atttttcctc ttcgtacgca tcaaacagaa gcatgagtaa
    25741 tgtcctatcc acccctccag aagtctcaac aatattgggg atatatctct caccagtatt
    25801 tggatctagg tatgaaagat cttttccaga gaatttactg tgttgtgtta gatcgtaatc
    25861 tcccctccag tgtataccct cattttctgc ccagccccat ggagcaagat attccacatc
    25921 ataggcatcc ttagcataga aaactagttt ttctttagga tgttgtgtta atctcaaatt
    25981 ctcctgtttt cggtaaagtc ctttgtaaaa tttcattctc tcctctttcc agtattcaaa
    26041 ccatttctcc atctcacttg gatgtacgaa aaattgtaaa tcccactgtt caaactctct
    26101 agttctgaat gttaatctgt ttggagtgac ttcgtttcta aatgctttac caatctgtgc
    26161 aataccaaaa ggaatttgtt gtcttgtact atctagtaca ttcttaaaat ctagatatat
    26221 tgtttgacat gcctcacccc ttagatatgt tttcatctta gaatcctcta acacccctac
    26281 ctgtgtttca aaaagaagat tgtatgacct tggttctgtg aattccgtat taccacaatt
    26341 ggaacattta tctgtaatat cttctggtct aaatctttga tgacacttct tacattctac
    26401 aacctcatct ccgaaattct cgatatgtcc agaagcttcc catacctttg gatgtgtgat
    26461 tattgcacca tctatcccca caacattctc tctcctatct acaatattct tccaccagta
    26521 tcttttcagg ttatttttca tctctacacc tactggacca tagtcaaaga aacctcctat
    26581 accaccatat atctcggaat ttggatatat tattcctctt cttttgcaca gtgcggttat
    26641 gttttgtaat aggtccatgt atatagaagg taactttaag tttttaaatt atatattaaa
    26701 gagtaagtta tttgaagata gttctatgct ataatatccc catatattac ataacttttt
    26761 acacattgtt caatggaaga ccagcaaaaa agtgatctac taggtcaaag agaggtaaga
    26821 ttagaaaaac ttgaacaatt agaaaagtta ggaatcaatc catacccagc gaagtctttc
    26881 aaacagtatt ccaatggaga gataaaaaat gagtttgaaa agtttgaagg gaaagaggta
    26941 atccttgcag gtagaatagt ggcatggaga gagcatggta aattactctt tggaaacatc
    27001 caggatcaaa cagatacaat tcaattaatg gtaagagaat cagatttgca ggaagattta
    27061 acaaaaggat atcttggttg ggataaatta gcacttttgg atttagcgga ctttgtagag
    27121 gtgtatggaa caattggaaa gacaaaaact ggtgaaatat ctatcatggt tagaaatatc
    27181 cgtttacttt caaaagcact aagaccaatt ccaagagaat taaaaaacaa agaccaacag
    27241 tttaggagaa gatatttaga tttggttata catcctgaca aagtaaagct ttttgaaagg
    27301 aaagctaaat tctggcaagt acaaagagac ttcctaaaga gtaagggttt tctagaagta
    27361 gaagtacctg tcttagaggc tgtaacaggt ggtgctgatg cgaaaccttt cattactcat
    27421 cataatgctc ttgaccaaga tttcttcctt agaatatcta cagagcttta ccagaagagg
    27481 ttacttggtg gtggatttga aaagatatat acctttggtc caaactttag aaatgaaggt
    27541 atggatgaag agcatctaca agaattttac gacattgagt ggtattgggc atatgcaaat
    27601 tacagagata atatggtttt ggtaaaggac cttcttttag agatagcgaa caaggtatat
    27661 ggtaaaacag aatttacaac aagaggacat acatttaatc tagcagacga atggaaggag
    27721 atagattaca ctcagattat taaagaaaga tttgatatag atatatttaa cgattcagat
    27781 gagaagatac tggaagtagt aagaaagaac aatattgaac tcactggaga tattaacaga
    27841 aacagactaa tagataacct atggaaaata attcgtgcta caatatctgg ccctgctttt
    27901 cttgttaacc atcctaagtt tgtttctcca cttgcaaaag cacataaaga gaatgagact
    27961 ttaacagaga gattccaagt aataattgca gggagtgaat taggaaatgg atattctgaa
    28021 ataaatgatc caagagatca attagaaaga ttcttggagc aacagtcttt aagggagagt
    28081 ggagatgaag aggctcagat gttagatata gattttgtag agatgttgga atacggtatg
    28141 ccacctgcaa gtgggtatgg tcagagtgaa agaatattct ggtttttgga gaatctaact
    28201 gctagagagg ggacactgtt tccacagatg agaaatgaga tagagactac aactagagag
    28261 atatatgaag ggaaagtaaa atttccaaag attgttaaga aagattagta gaaatttgtt
    28321 accaaccccc agtttatgcc attcaaaaaa cgaatgagta cgcacaatgt gcactggggg
    28381 ttggtttggg attaggattc atacaaagct gaaggtaatg acaaattcgt catcatcttg
    28441 cttcatacac cctacccaaa gagtcctcga tggaacttga atccataaac cttttcctta
    28501 caaaggaagt gctctgccaa ttgagctacg aggactacaa taataacaag gagtgagtac
    28561 aaggagacca aggacaggag ttatgcatgg cgttacaaat cacctcggtt ttttatccca
    28621 gcactcacat gtgcgaggaa acacccttgt tattccaata tctatttaaa agaacagaag
    28681 aattatatta taaatatacc ttataggtca atataaaata actaagatat ttctgctatc
    28741 atattcctat ggactccata aaagatatcc ttaatcaact taaaaaagac aacgaacaag
    28801 aggaagatga aaagcaacca tcccttatag atactcaaca attagcaaca aatccaaaca
    28861 aactattcgg taataaacat aaatatgtaa ctacagaata ccaagtatat ggtttaagac
    28921 tagctggtaa acttggggac aagaaacgtg cgactatgta tattaaatgg gcaaaagaga
    28981 aacctagggc tatactagaa atggcataca gctttgctat agactatcca aatgctaaag
    29041 acagaagccg aatatttatg tggaaagtaa aagagttaga aaatgaaaga aaagaaggaa
    29101 aaattcaaga agatactaaa tagagaagta ggtatgtatc tctcaaaaga gttctcatat
    29161 agggggcaga tatttgtatg gataatagca gatttcataa aaatagtagg tctatgcttt
    29221 gtatggatag cagcttctag atctaccaat gatatatctc aatcttatgt aatctcctac
    29281 tatctgttac ttcttcttgt aagtaaatta acaacagatt ttactccaga atatggatta
    29341 aaaaatattg ctagtggggt attttctaac tatcttctta aaccatttag ttatcttact
    29401 gaatatcttg gtattaactt agcatcaaat gttttaagat ttcttatctt tctccctgca
    29461 tttgtatttg gagtaattat tgcctcgaaa tttgatcttt taatatttgg ttgtaatcca
    29521 tatcacatat ttcttgcact agtggcaata gtattaggct ttctcataaa ctttttactt
    29581 gggaatattc ttactttgat atcatttaaa attaaagaaa cagatggaat tagaatcttc
    29641 tactacaaca tagcagcgtt tctttcagga gagtatgtcc catttatatt tctaccattc
    29701 gttcttcaat ttggattaca aatgttgcca tttagataca cactctcttt cccagttgag
    29761 attctcacag gtagattggg atcatatgat cttcaattag gatttctcat tgcaagtata
    29821 tggttgattg tactctggct tgcatacaag ttgatattta agttatctat taaaaaatat
    29881 gaagctgagg gtatttagcc attacataaa agtatggtta gttatagtga aagctagctt
    29941 tagaagaagc tatgtgtaca gaacagaggt attggttaga ttcgtaagga cttttttcat
    30001 acttggggtt cagatacttt tactaaatgc agtctttggt gacaatgagg tgtatgtagg
    30061 atggacaaag aatgaagctt atcttgtaat gggtatatgg aattttctta attatatggg
    30121 ctggtcactt tttggtgtaa acctattcaa tcttgaaaac aaagtaatta gtggagagtt
    30181 tgatttctat cttcttaaac caatctcttc agcatggttt gcatctttct gtgatttctt
    30241 tatttacaat tttgtaactg tactatctgg gttgtcatta attgggtggt atatatatca
    30301 ggagtggagt aatttagaat atacaaatat catattgggt gtagttgcaa tatttatcgc
    30361 attaatactt tggtatggtt tctacctact ctttgctagc ttcactatct ctaatccgag
    30421 aaatggttat cttgttgtag caaaagaact tttaggtatt acaaaatatc caattgatat
    30481 atttggtaat acatttcaaa tattattcta tactttacta ccaatagctt ttctaactac
    30541 tgtaccagca aaaacattca tgggtgaggg aagctatatt gttttgctct gtggattact
    30601 tgtaggaata gctactgtaa taattggaaa aaacatatgg aacagaaata tacgaaacta
    30661 tattagctct ggtggttaga tactgctact agaactttgt ctaatacctc tttcgcagat
    30721 ttaccagtca ttggaactgc agatgcatat acatgacctc caccacccaa ttcttccgca
    30781 atattactca catcttgata cccttttgta cttctaaatg aaacatacca actatcttct
    30841 ttgtcgaaat ctggttttat aatgaacccc cagtgtacac cctgtatatt tctaatatat
    30901 ctatctctca aaaaactttg agcttcattt actcccttct gttttatatt tatttcatcc
    30961 acatcttctc tatctatata catatatgcc atgtcttttt ctatggttaa agattctaaa
    31021 tatttgataa tcgcaggagt tgtttctttt ggaaatttag tattcttttg tgtaaattct
    31081 tccatatcta tgggggagat atcttttaca tctgcgaata tcctaaaagt gtcagagcta
    31141 gtcatagagt agagaaatct tccagtatct gaaactatcc catactgtat tagttctgca
    31201 acatcctttg aaactcttaa tctttttcca aacatatctt tgaatgtagc atacacctgt
    31261 tctgtagcag agcttcggta ttcattaatt gtaaaaattt cctgtgcttc tacttcaacg
    31321 ctatgatggt ctaggtatac aatattggaa ctttcaaacg tatctatccc atcctcaaga
    31381 cagagtgaaa gattagatgc atctgtaacg attactaagt cagcctcttg tttctcaatc
    31441 ttttcaacga tgttaatatc tttcatcata ggcatattaa ttgtaggtaa ttgtcttgca
    31501 agtaaggtta tctctttttt agaaaaatgc tcctttaaaa agccgtatgt agctagcata
    31561 ctgcaaaagg catctaaatc tggtccttta tgtgtaagga ttaagatact cttagcgctc
    31621 ttaattcttt ttttaaattc ttttgtctgt gatttcatat tcttgataat accaagtttg
    31681 gagaatataa taaataatta gtaacaagat ataaattgag gatatatcct aatgatagat
    31741 gttaaaagaa tagtagaaga gagagagtat gtggaaaaag ctttacttaa gagagtcgca
    31801 aaagagaatt tagacttgga ttccattgta gagttgtata acaaacgaag ggatttacaa
    31861 atgcagtttg acacaaagag agctcagcag aacagtttca atgaaaagat ggcaaaggtg
    31921 gagaaaggga gtgatgagtt taaaagtaca gtagcagaat taaaagaaaa atcagaagag
    31981 gtaaaaaaga ttgaagaatc tttaagaatg acagagaaag aacttaagga gaagttagat
    32041 gttcttccaa atattccaga agaggatgta gtgggtggtg gaaaagagaa taatgcagtt
    32101 attaaagagg ttggaaagaa gccaactttt gattttgaga ttaaggacca tgtccaactt
    32161 ggaaaagaat taggaatctt tgactttgaa acagcatcta agatagctgg aaacaatttt
    32221 gcaatgtaca ggggacttgg tgcaaggtta gagtgggcat taatcaattt cttcatatca
    32281 aagcacatag aagatggata tgaaatggta atccctccaa atcttgcaac agaagagtct
    32341 gcatacatag caggacaact tccaaagttt aaagaggatg tgtactgggt tcaggatggt
    32401 ttgtgtttag tgccaaccgc agaaacagtt cttacaaata tcttcaaagg agacatgctt
    32461 gaggaaaaag atctacctaa gaaattcttt gcatatactc cgtgttacag aagagaggcg
    32521 gggacatacc gagcaaatga aaaaggatta attagggtac accagtttaa caaagtagag
    32581 atgtaccatt tcactacaga ggagaaatcg gaagaatctt ttgaagagat ggtaaacaag
    32641 gctgaggcaa tagttacagc gctaggctta cactacagag ttgttaaact tgctgcagga
    32701 gattgtgctg caggtgcggc aaagacatat gacattgagg tttacatacc ttccatggac
    32761 atgtattacg aagtaagttc tgtctctaat gttaaggagt accaatctag aagaggaaat
    32821 atgagataca aaccagatga tggaggtaaa ccaaagtata tgcatactct taatggttca
    32881 ggcttagcta caagtagact aatggtagct cttgtagaga cataccagaa tcaagatgga
    32941 agcataacta tcccagaagt actccgacca tttatgggga atatagataa aatttctaag
    33001 taattagaat gagctttttt ttcggttttt tcccagatga aaataccaat tacaagataa
    33061 ggaaggtagt aggggaagta ggaagagttt ttgctgactt ccagattcct gttagatgga
    33121 gtaaaccaga aacatatcac atgactgtat tgcatgtagg agagagactc tccattttca
    33181 aacggttagt atggagattc aaactaaaaa caatatgtat aaaaccattt aaagtatctt
    33241 ttaacacagt aaggttaggt atctcaagaa aatataaaga gttagtatat ctagatacta
    33301 atgaaggagg agatgtaatg agagagatgc ttctagatat gagaaaagtt ctaaaattga
    33361 aggatgaggg taactttgtt cctcatctga ctcttggtag ggtaagtaaa gatctttcag
    33421 acgaagaata ttccaattta tgtaaagatt tagcagtagt tgcaaaaggt ttagatattg
    33481 aaaagattga attttatgta aatgatataa aattaataaa aagttctgga gataattatt
    33541 cagtcttgat gaatttagta gatctttcca aaactaaatc ataattatcc acccatattg
    33601 catctttatt tcttttaaac caagtctttt gtcttttagc ataccttttt gtatttttaa
    33661 taatctcaga tttaacatcc tcaatcgttt ttgtaccatc aaaataccca gtaaattcct
    33721 tgtatccaat tatttttgaa actgtagaat tgaaaaggtt gttatcttta agataaatat
    33781 tttcttcgag taatccatca tcaaacattt gggtaactct ttctgttata tttttttcta
    33841 aatgtctttt gtctacattc ataacaagat aaaggcaatt cattttctca cctctttgct
    33901 tgcctaattc tcccttctca attatccgtc ttaatctcac tgggtttacg atatcactct
    33961 cattcaattg atttagtatc gttgagtcaa ttagtgagtg aagctcttta atagatttgt
    34021 tttctaaatc ttctctttgt agggaagcat cttcctcttt aagtgtgtag ttgtaaatta
    34081 cagagtcaat atataatcct gtgcctccca ctatgattgg aattttacct ttaggaagat
    34141 tgtttaaaat ctctttaact tctttttggt atttgtagat attgtatttc tcttttactg
    34201 agatatgacc gtagaggtaa tgagaaattt tttcgaattc actttctatg ggtcgtgagg
    34261 ttccaattct aagttctttg tatatctgtc ttgaatctcc attaataatt actccgttaa
    34321 tattctttgc taactgcaaa gcaattttgg atttacctga agctgttggt cctgcaatta
    34381 caatgaccat gctatttaat tagtctagga agaagtatgt aatttaacca gatggttaag
    34441 attgaaacta ccagagagga tattagtaga aaaaggagtg ttgaagacac gaagattgta
    34501 caaagacaaa acaatgtaat tagtacaaag gaaagtttaa atgtgtctct taggaattca
    34561 gtgtacagta tttcaaatgt gaagtttttg tttctcgaat ttagactgta aaatccaacc
    34621 aaagttccaa gatttaaaat atttacgaaa attacaatga atggtgaaag gaggaaaaac
    34681 tctgaaatcc agatgtcgaa gacaatccta agaacaatga aagaaagaat tgttagtaat
    34741 cccgatatgg caagagagac tattgaaaaa agagtaatat gtttacttct tgtaaaaaga
    34801 tatatacaaa gaggaacaat tgcgaaacaa agcaatgtaa ttacaagtgt taaaagtttt
    34861 tgatatgttg tccagttgtt ccagaatgct aagaatatat ccatatttat atctttctta
    34921 ctttatttat ataccatcca gatagtagca aggttatctt ggaaatggca agtaaggcaa
    34981 tcatgtttct tgctactatt gatatgtatc caaaccctaa gaatgtcagt actactaaaa
    35041 ctccaaagag aataatatct acatatacta cagaatcttt gttctcagat attacacgaa
    35101 ttagaaccat agtgagtata gcctctatag gtagaagaaa tgtatctaca ggaatctgta
    35161 agaatttgag aattgtaagg tagagggaaa tcgttagaat tgtagctaag aatgatttta
    35221 tcagaacagg tttagatgca ttcttggaaa taaatagata tatatatgag ataagtaaac
    35281 cagcaaatat taaaactgaa accatgatgt aatttgtttg tggaatattt ggaatcatgt
    35341 ctttttcgga agttaaagat atgttagcag gaatactgcc tgagttgtaa agaatatcta
    35401 ttatgttaat ttcctgtgga tcctctgtag atactggagg tgcaaataac ttgtttggac
    35461 ctgctggaac ctgtacggga gaaacaaagc catctgtgct tactccgaca gtctgtccct
    35521 catactgtgt taagaatttg ttgaattcta cgctctttgc tggccaaagt ttaaaaattg
    35581 caaagtacga ctctgtacct gcactgtttt ttaattttgt aatatataca tttctaaaat
    35641 catctctatt ccaatctgta ctgttgtagt ttgttgcaag aagatatgca tatggatttt
    35701 ctggatcttc gtagtttaca tcgtcctttc ttgtaacaat ctctactgcg aatctgtttt
    35761 taattagttg ctctgttaat tctttctctt tagaggtggt aattgtaacc atgatgtcat
    35821 ttcccttttg agctacagac atctgttcaa ctccgaattt ctgtaatctt ttaaaaagta
    35881 tatctcttgt tttctggatg tctttttgtt ctttagaatc aatagtgaga agatattctt
    35941 ttgaccaata ggaagaatct ttagtattaa gattgtatgg tgtgttgttt agatatttct
    36001 ccgtaagagg tagcgcaatt agtattgtag ctacaaatac tgaggatatt aaaattacaa
    36061 atatgtaatt tagcagagac gctatattta tgttgagttt tagtttcggt aacttcattc
    36121 tagagtaaat cagatatctg tccaaacaga attatcttat cgttttccaa gatattcatc
    36181 aagaagggtt ctttcttttt tctagcgaag tcaaaatctt cttttgttat agcactaaat
    36241 cgaatctctc ttcctagctc tttctctact tgtgcaattg cattagacaa atcttttatc
    36301 ttcattgttc ctattacgag taaatctaaa tcagtaggat tgttatattc gttttttagg
    36361 aagctgtctg ttaggacaat gacctgtata ccttcaactg tagctgtctc ttttaatatt
    36421 gcagtcccga ttttagactc tttgaaaaac atagatctta gttcccaaag tattgaacaa
    36481 tctttgttaa gtttgtaaac aagtttatta ccttctttat gagatttcag aataccagca
    36541 tcttgtaaat taagaagctc tcttcttact gcattaattt cttcatccag aattctaact
    36601 aacccacgta catgaaatga cgcattgaaa tttgtcatgt attggtcgag aattttaact
    36661 cggacctttg atataaaaag attctttagc atagttcccc aacctgctta atattattgc
    36721 tcaattatat caaattttcc gtaagtttgt ggaaccatag caatccaaat catccctcgg
    36781 ttaaaggata tctcttttcc tgaagaatcg tagtacatag ttctatctgt tctactcttt
    36841 ttcttccatt ttccatctat gatttttcca tcctgaagta tctttgcatt tccctctcca
    36901 attgttgtga taataatatg gctctttgaa tctccagtat aattcatctt cacctcttgt
    36961 attacgacat tctttgcact aatttgtttg tttgtctctt gatcaatgtc agcctgacca
    37021 ccgacttttc tgaagtatct gtttgtaccc ttgtcgtatg tccaaatggc atcgtactga
    37081 cctccgtttg gcagagatgt atgaaagact gttttaacaa cagatttctc tcccctgtct
    37141 actatctcag catctctttt gaatttccat tcatcaattg ttgttgggaa attattccat
    37201 ttatatttat cagccttttc ccatactcct ttaactgaag tgtaggcatt atgtggagca
    37261 tatcttctac catcatccca tctccagaaa agttcaatat ctgcaatttc tttgtaataa
    37321 tggatattac tgcatgcatt gactcttgga tctgtggaag aagcacagcc aacccttaca
    37381 aacaaaggac tgtattcact tgcccattct aagtaatact gtcttacact tcttacagaa
    37441 ccaaccttct ctggtccctc actccagtaa attgccatat gtctagtgat tcctccctct
    37501 gcatttgcct ctattacgat gtctgcacta ctcaatcctg agagtggtct agcatctgca
    37561 tgattgcttg acattaccgc gactggtctt ctttttttaa gtttatccat ttctgttttt
    37621 gtaaacagta ctccatttag tggactaact tctgttcttg gttcagatgg cttttgcaag
    37681 gaggtcagag atctagaaaa cggagaatta ctgtcaattg aggcaacaaa atcttctgca
    37741 aaaggaataa ctgtttgtgg ggatactggt tttactttgt agataccaaa ataaaagaag
    37801 agacccaaac cacaaagcgt aaggaatgcg acaattgaaa atattaaaat attacgatct
    37861 attttctttt tcttaggtaa tttctctagt actttttttt ctaactcttt tgtctgttct
    37921 gcaatggcag gagcttttgt ttcggttggt ttaatttctc ctttattgtc agatatattt
    37981 atctcttttg gcatgggcag agtactctaa aatatataac catagtatca ccatttcaaa
    38041 caaaaatgaa atagtggtaa tatattgata tataaaatgt aaatgtatta ctatgaagaa
    38101 gataatattc ataattgtaa tacttcttct cttaggagcg ggagcatatt tcatatttaa
    38161 gaaaccaaag gtagaagata ccaataatac agatacaacc gtagaggaag aacaagatac
    38221 tgaaactaaa gaagaagaga aagaggaatt aataattcag gatgaagaag actttacagg
    38281 tttctcagat gatgagcagg taattggtgt agctacagag gacttgttta acattgagtc
    38341 aattacagac gagagtaaaa ctggatacca tagattctct tttaacctct cgacaactgg
    38401 aacagatgat ccatatgtaa ctgctaaata caattctagt acaggtgtaa ttagggttga
    38461 attgaatagt atagatagtg acaagagtgg aatcggatac caaaaggaca gagcaattaa
    38521 caaagaaggg atagttagac tttatcatgc tgtaacaagt actgcggata aagaaattta
    38581 tgacataggt cttgcaaaaa gtacaatctt taaattatac aaacaaagtg gagatggtca
    38641 gcttaaggta atacttgatg ttaaatatcc cggagaggtt acctctacaa tagatttggg
    38701 tagtacagaa ttttctacag atctacaaga tatttctggt attgcaaacg caacaattaa
    38761 gagttattca tacagctctt ctggaggaat tctaaaattc gtatggagtg taactgcaga
    38821 aggagagaat ccaataccat ctgtaacagg agagtattta gatgacaata ccctttcaat
    38881 taagttcaca tctctaacat tagacagagt tgttaatgca gcaagtagct taacattacc
    38941 aactggtata accatagatt caagtaaaga gggttctgca agcatataca atttcatagt
    39001 tggttccaag aaagaataca aattaagtgc aacaacaagt ccaaatcagg taatattaga
    39061 aatagacctt taggtaacaa gtttacattg gtaaatagtg agaatcttaa gaattaagta
    39121 gaatatttaa tatactagag gacatacatg attcggggta tagtctgtga tttttacaat
    39181 gtgctttaca attcttcccg caaagaaata aatacagaag ttcttgatat tcttaaggag
    39241 tgctattccc aaaatattcc attatttctt tttactaatt cttcacttca attccttcat
    39301 tcggaaaata ataaaacaac ttttctaaat atcttttcta gatatatatc aatgtgtgaa
    39361 tataatttgg taaaaccaga tgatagagca ttcgagatat taattaagga ggtaggatat
    39421 gatccaatag atgtagtttt gatagatgac aaggaagaga atattgaaaa ggcaaaggaa
    39481 ttaggattta agacaataca gtatattgac cctgtgcagt tgaggaaaga attaaacaat
    39541 cttgatataa ttatctaacg gggtgtagtt cagttggcta gaatgcacgc atggggtgcg
    39601 tgaggtcgca cgttcaagtc gtgtcacccc gatttatccc aataggtctg taatagactc
    39661 tcttctattt accctatcta ttgtctcggc aaaatattta gcgacagata cctgcttata
    39721 ccatctacac tccttaagga attcttttgt ttggaacgtc gcattagtac caataactaa
    39781 atcaagaatt cctttctcga atgcgctatc gaatctttct ctagcaggat tattaaacaa
    39841 agataatgta caaactgcaa ctatcttttt tgctcccatc tctttcgctt cctcaattgc
    39901 tcttaccaat gttccaccag tacaaatcat atcgtcaatg atgtaaacat ccttgtcttt
    39961 aacttctcca agaatagcca attcttcaat tgagtttggt ttacagtaat ttcttttttt
    40021 gtagcaaaat gcaatatccg tttttaattg atgagcataa taatttgccc tttttgcccc
    40081 acccaagtct gttggcataa caacagaatt ctctgtgtct tttattaact ttttcaaata
    40141 aggcagtaaa acatagctac cttttaaatt atcaaccttc gctcttctga aaaatccctg
    40201 tatagctgaa ttatgtaaat ctattgtaat tatatgatta actgccccat caccctctaa
    40261 ctcatatgca attcttgatg ctgtgattcc ctctcttcca tcctgtttat cttgtcttgc
    40321 atacggataa gaaggtaata cggtggttat tctttcggca tcacatcttc tgcaggcatc
    40381 tacggttgtg tatagtgctc ttagattatc atctactgac ataccctcac tatggttctc
    40441 tacatcttgg attatgaaga cgtccttacc tcttatgctt tcatttagaa cagtttttat
    40501 ttctgtattt gcgaataaaa tttcggttga gtctattaaa tgtactttat tttcagatat
    40561 ttttcttaat tcttttacta ctttctttgc aaattctaga ccggatttac aagaaataat
    40621 ggacaggggt gctttttgtc gggtcataga gtatttttat tatcttaatt attcattata
    40681 ttggaaagtt tggagatttc aaaatttagt gataatatgt aactcataaa gttttcattc
    40741 tcttaatgga aaagaaaagt aacatcccat ttaaagaata tttaaaaata tctctttgga
    40801 gcttaaaggt tacttgggga atatccccat ttgcagcaat tgtaattctc tctacagaaa
    40861 tcattcgtaa tttaagaggg attgtaaata catatatcat tgctaggtta attgatagac
    40921 ttatctcttt agcatctaac gatggaacta aagtcctaga tattcttcct tttcttgtga
    40981 ttattcttgt agtcaacatc ctctttacag ggatagacga actaagaaac tatgcaagta
    41041 gaattgtaag gagattgtct caaccatatc tagaaaaaat gtcatttgag aaagttaatt
    41101 ttcttggagt tcagacatta gaacttccag atgtagcaaa tgcaaaacaa aaaatgcaag
    41161 actggctata ttcaattatg gatgtaggga ataatgttgt aagaataatt gctgcatttg
    41221 taaaaactct tgttgcagga gtcgttctcc ttaaaactat ccctctaatt attcctttaa
    41281 ttttcttaac aactgtagtt ttctatttcc aaagaagata tttcttcaaa aaagaatttg
    41341 attggcaaac aaatgacaaa catctcatag agagaagaag gagctactgg atagcagaaa
    41401 gactttcaga cagtacaagc attggagagg tctcaattac tggggcatac aattacctaa
    41461 gtagtaaatt cacggctttc tttaaatatt acaacgaagg gctgatgaag atcatgaaga
    41521 aggatgcaat tagtagtttc ttaatatcaa tcttgagtaa tttcgtaatg atagttggat
    41581 atgttcaagt attccagttg ttgctactta agaagattac aattggagag accacctttt
    41641 atatgtcaac gatttctaat ttctatgatg gtattgaatg gttcttctcg gagattgttg
    41701 cttacagaga tttggtaatg aagatgaaag aggtttacga attcttcggt ctaaaaccag
    41761 cgattgaaga tggaaaagaa cctttagaaa gatttgtaac tcctcctgta attgaagtaa
    41821 aaaatatatc tttccattat ccaaatagta agaggaatat ttttaagaat ttctccctaa
    41881 aaattgaggc tggagaaaaa ctagctattg ttggtgaaaa tggtgcaggt aagacaacct
    41941 tagttaagct tctttctagg atttatgatc ctcaagaggg agagattttg ataaatggaa
    42001 ttaatctcaa agatattaag attaatgatt ggtacaagaa tttgggtgtt ctctttcagg
    42061 aatacaattt ctatggagaa ttaactgcag aggagaatat atatatgggg aagtcaatga
    42121 aggatataaa tagaaaaaag ttaattgaag catcaaaaaa tgcagatgca catgatttta
    42181 ttaagaagta tgaaaatgga tacaaaaccg taatgagtga aagatttaga ggaggcatta
    42241 gacctagtac aggacagagg caaaagatag ctatcgctag attcttctac agaaatgcac
    42301 ctgtagctat tttcgatgaa ccaacttcag caatagatgc agaatcagag tacagaatat
    42361 ttaatagaat ttataatttc tttaagaata aatctgttct gattatctct cataggttct
    42421 ctaccgtgag aaatgcagat aggataatag taatacatga aggtaagata gtagaagagg
    42481 gttcacatca agaattgttg aagatgaaca agagatacgc aaatgcattt aagaagcaag
    42541 ctgaaggata tctgtaactt gatttggcta atgggtatgg tataatagta atgggaaaca
    42601 acgcctatca cacagtattt gtgagttatt accggccatc cagggtcggt tttttgttaa
    42661 atcgactctt ccgtttgtgt gtcagaggat cactggcaca taagccagaa ggttggagta
    42721 gcccgatcaa ctacaagaac cttctgagcg aaaactcttg gacagtatga tgaataatct
    42781 actggacagg gggtgaagcc aaagagcagt catcccctgt attccaaggg gatgataaaa
    42841 acttctttat ttacaattaa ttatttcctt ttccgttatc tttattatct gtattcttct
    42901 cctcattctg ctgctgattt tgtgtattct caccagtatt ctttaaaata ttcttctcct
    42961 cctctatctc ttgttccatc tcttctgtta tatctttatc tacatcctta atcttcttct
    43021 gagctttatt tgcagtttct aactgttgat caagatgctc aagatatctc tctcttgcag
    43081 tttgaatagc ttctgcagca ttcgtattcc ctttctcctc ctgtttctgt actacttccc
    43141 ctaatctctc atatgctctt aatctttgct gtgccattaa cctaactgcc tctccaagtt
    43201 cctcatctgt aacatcctca tctcccaaca tagtttcaat ctcctcttgt ctctcttcta
    43261 atatggcctg ttctaagttc acctttgcta catcatctaa agttaataat ctttgtacac
    43321 tctcaaatgt agtatctaca gcaaacaaag aatctgctgg tgttgctgca tttgctttag
    43381 tagctaagaa atagccacca acaccaacaa ttaacgcaac ggaaataata atacctaata
    43441 ctttactcat acgagtttgg tatattaatt taagtatatg taaatatgct acaactttag
    43501 aacctaattt caagattaaa gaagatttaa cttgaagaag taacatatat ttgtaaaaat
    43561 atattaagtt aagttaaaca agaagaaaca tgaaagtatc catagacaag agtaagtgta
    43621 ttatgtgtgg tagctgtgta gctatatgtc cagaagtatt tgaaatgaaa gatgatggta
    43681 gtgtagatgt aaaagaacag tacaaagaaa aagatatttc agatgatgca ataattgcta
    43741 aagtaaaaga agcagaggga gcctgtcctg caactgctat tgtagtagaa gattaaatat
    43801 tatggatgat tgtatattct gtaaaattat taagggtgaa ataccatcat acaaggtgta
    43861 tgaggatgaa aatgtttatg cattcttaga cattaaacca ttaagcaagg gacacactct
    43921 tgttttacca aaacagcatt acaaagatat tcaagatatt ccagatgaac ttctgtgtga
    43981 ggtgaataaa gtaagcaaga gaatagtaaa aaagattcaa aaaaagtatt ctcctttagg
    44041 cttcataatt aaccagaaca atggagagag agcgggacaa actattgccc attaccattt
    44101 acatattaaa ccagtgtatg agggtactgt gattcccgac gagatgaacc ataggagaga
    44161 gctaagtaaa gaggaaatga atggagttct gaaagactta attgaggaag aaatctagta
    44221 cctcatctct gtactctgta tctaagtcga aatgagtttt gccatctaat tctattagtg
    44281 taatcttttt tcctaattta tccatactgt ttacaaaact tctagagttt gtaatcccta
    44341 tatttacatc ctttgttcca tgccatatct gtatatcaat accatctaaa acatcaatgt
    44401 tactcttgtt ccactcatat gatcttgtcg tgggtgcaag aagtgcaacc ttggatatat
    44461 ccttaggata ctttgcaaca tagtgcatag caggaagtcc tcccatactg aatccaatta
    44521 cgaatgtctc tctggaagtg ctgtagttat ttctaatcca ctcaaccata ttaaaattat
    44581 cttgtatgga agcagcattt ccccagttct catcatgggc atttgaagct gcgaatatca
    44641 ggttggattt agtatattct tctccatata tctgtagatc tttcataaat ggatcttcaa
    44701 ggttctctgt aactctggta tttgaaccgt ggttgtagat tacaattctt gggggatcgt
    44761 tttcatctat atttgttggt atagcgatat atgcaatctg tccagatatt gcaaatatct
    44821 tttcttcata atgtggacca tctggaatct cttcttcctc ctctatgact tctgtctttg
    44881 gagtttcctc ctttacttct tcctgttgtt caggcttatt attcacaaaa aagatttggt
    44941 aacaaagata tccagggatt gctatgaggg caagaaatat taaaaagagg ataatatcac
    45001 taaaggatat tctacttttt gtcgtttcca tattgcccat gtcttcatta tatttatttt
    45061 tttatatact gtctagaaga tttgagatgg taatctgaaa cttgtataat tagatcaata
    45121 aatctgggat ggaaatgcaa aaaggctctc cctctcccag attttttcat atccgtttaa
    45181 atatgttttc taataattaa gaattaatta attttagaaa attatttaac atggtataat
    45241 gtgtttcatt ctaactttag ataaaaattt acatgccaaa gacaaagaac aagaccgagg
    45301 gagagatagt tatcaaccta aatacatttg caattccaat tgccataatt gttgcaggtt
    45361 tgttaattgc agttggtata tttttcgcta acaaaggaaa agatacccaa caaaatattc
    45421 agggtacaga gacaactact gctacagaag ataagtttac agctacctct acaaccgtag
    45481 atgatgatcc atatcttgga gataaagata aggttaaagt tgcagttgtt gaatttagtg
    45541 atttccagtg tagttactgt aaaagacact cagatgaagt ttttccttta ataaagagtg
    45601 aatacataga tactggaaaa atagtatatg tatttagaga ttaccagttc tttggaggtc
    45661 tctcaattga tgcagctatg ggtggtcagt gtttgtatga cttgactggt aaagacaata
    45721 gtaaatatgc tgaataccat aaaggtatat ttggtttaac aagtaaagat gcaataacaa
    45781 cacttgcaaa gacattaggt gttgatgagt ctaagtttaa agcatgtatg gatgcaacta
    45841 aatactccga agaaatcagt aaggaccaaa cagatggaac agcagcagga gttacaggta
    45901 ctccaggatt tgttgttgga gttctaaaag cagatggaac agttgaaggt aagtttgtag
    45961 gtggagcata cccaatagag agctttagaa cattaataga tgaaatgcta gctaagtaat
    46021 catcttgatt tgtaattgat aaggggaggt ttaatacctc ctcttttttt acttcttctt
    46081 tttcttgtta atcttcttta cattatcaaa agaatccttc ttcattctta caactgctcc
    46141 tactgctgtt gcagatatag ttatgctgta aaatactcca aatactggta caagagaaag
    46201 aagggtggtt aatactctac ctgtaagtaa tcctttgtat ggtctgtacc cctttgtccc
    46261 gaaaagctcc aatatctctt gcccaagagc actttccaca tagattgttc caaatgtagt
    46321 tagaaatagt agtactccaa gaatcaggag tgcaaaaggt atgccaacca aagtaaaggc
    46381 aagaagaaat agagggagtg gaatcaagag aagtattgca atccctaccc ccaagctttt
    46441 aagaaactct gttggagatt tagatacctt gtttacaatc tgtcctgttt taacaggcga
    46501 gaggaaaatt aacacatatc ctgcaattga aaggcctaca aatgttagaa atgcatttaa
    46561 tatttttaag ctataatcag atactctagt aatttcagtt cttttcgttt gctcagcctt
    46621 aagatctaca ccctgttctt tagcaatatc ctttagtgtg tttgtattgt atatattccc
    46681 tgttacattc tctttgagga tacggtactg acttgcaagt actaaagcat ctccttttac
    46741 aacgctttct atggaattag tatatccaaa tgctctcaaa tcatccccga cagttccttt
    46801 catatccgag ttcattgaaa cagcaagaag atcttttcct gcatgtccat catatattag
    46861 agatgcactt aagacacatg cattgttact caaaacccca gatatattac tttctccagc
    46921 aattactact aagtttccat atatatttcc atcaatggtg acatcggaac caactacata
    46981 catatctcca gttacggttc cctccataac tactttgttc gaaaggacga aaagatctcc
    47041 atctattaca ccatatacac tagaattact tcctagtagg tagacatcct cttcaatagt
    47101 ggtttcttgt tctactgttt gttctttgaa aaagaattgt gcagcttcta ctttatatgg
    47161 tagaaaaaag attacagata caaagagtaa aggtaaaact ctttttaaaa tgttcatact
    47221 aaaggcagta acagattata tatacatata tagtagcaaa tctttgcaaa gagttataca
    47281 tatctttcca tttgacatgg ttagtagttt cttagtagta tattactatg aagaaaaaca
    47341 aaatttacag tacaactgaa ttttacaaaa atccatctaa aatacttggt tatgttaaag
    47401 atggagaagt tgtgtatctt ggttacaaaa aattaaagga tccaattgca gtaattacac
    47461 catacaaaga ttacaaacct aaaaaagaaa gaatttctct ttggaagctt tcttctaaaa
    47521 aatctgtaag tgcaccagaa tatgcagata gtttgaaatt tatacaatct caaagaaaaa
    47581 gcagatgaat ggatatgttc tagatacaag ttttctaagt gcattctaca atacaaaaga
    47641 tgttaatcac aaagaagctt tggagatgtc tagaaatata gatgtaggca atctcttaat
    47701 tccaatcata gtaattgcag aattagcaaa tttcaagaag aatgaaaaat ttagaagact
    47761 cctgttaaag gaagccatga acatagcttc agggattcca tcattagatg aaaatgattt
    47821 ggaagaatat ctttccttca tagagaatct cccaaataat atgacagcaa tagattcgat
    47881 gattcttttt ctgtcagtaa aatacaatgc tgtgttagta tcttttgaca aaaagatgat
    47941 gaaaatagca aacaatatct agagatatcg ttaaatattt acatacttaa ggtataatga
    48001 gtggaatgaa aaaagttctc aacattatac aaagatacta tccagcagaa ggtggtgcag
    48061 agcgtttttt ctggacactt tcagaatatt tagcaaatga tttaggcatt gaggttgatg
    48121 tttggactac agatgctctt aattctgaaa cactttggga tttagatgga aggaaggtag
    48181 aaaaaaaaga agaaa
//
LOCUS       DAOQWP010000003        36908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_1313_length_36908_cov_8.234174, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000003 DAOQWP010000000
VERSION     DAOQWP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 36908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36908
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1313_length_36908_cov_8.234174"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..819)
                     /locus_tag="PKI16_00800"
     CDS             complement(343..819)
                     /locus_tag="PKI16_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53011.1"
                     /translation="MKKKKISSTRKKEDQNTQLGLKIKEPFKNKFFKIKGKTDSLLIQ
                     LEVRKFLKDIFFWFVLVISLLMIAYQVYIIVVNLNSLPSLLPILRYNIAASAKLTSKI
                     YLVIYPVISLVSLATATFLTIRVYNREKFLTKLLLLSALLCCVSMTISLIQLINAF"
     gene            complement(828..1568)
                     /gene="rsmA"
                     /locus_tag="PKI16_00805"
     CDS             complement(828..1568)
                     /gene="rsmA"
                     /locus_tag="PKI16_00805"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNR53012.1"
                     /translation="MEFKKSLGQNFFINENQGNQIISYISNESCQYVVEIGPGIGFFT
                     IKLKEMGKNLLAIEKDNVLAEKLVAQGINVLNVDFLDWDFKELNDKQVIFFGSLPYNV
                     SKQIISKIISSQYFTNNCYFIVQKEVAEKYIAKVPDSNLLSLRTQIYANCKKILDIGP
                     SSFKPSPKVNSSLIMFSPTTKTLPIDIPSFNSFLEQCFTHPRKTIHNNLREYKFSSDF
                     DKLLQKRPQHLSLEEYLQLFNDKSSVLV"
     gene            complement(1569..2501)
                     /locus_tag="PKI16_00810"
     CDS             complement(1569..2501)
                     /locus_tag="PKI16_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNR53013.1"
                     /translation="MTKVTDYDFFGYDYANYWSNRRYENEAEKILLTKIFSNKNGNWF
                     LDIGGSYGRLSSTYYKKYTNPIILDYSLQTLVKNKDILKTKYPNIQLIAANAYKLPFK
                     DNTFDGALMVRVLHHIEKPSAYFEELHRVMNRNSFYIQEFANKVHLISTLKALVRLNF
                     KFFSKEPYQQPTKGNFEGTKSGEEAIFFNYHPKYIKELLTKKGFTVIKKHGCSYIRSA
                     LLKKVFGEELMILTERILQPLLSWSNIPPSIVFESIIKKEIKENVVHTSELEDILACP
                     KCKGILSIVNSEAKCKKCSLTFVKENDIWDFRIK"
     gene            complement(2503..4227)
                     /gene="dnaG"
                     /locus_tag="PKI16_00815"
     CDS             complement(2503..4227)
                     /gene="dnaG"
                     /locus_tag="PKI16_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNR53014.1"
                     /translation="MDQNNQIEEIKNRLDIVQVVGKYVQLKQAGKNFSGLCPFHKEKT
                     PSFIVSPDIQRYKCFGCGETGDIFNFVQKIENIDFVEALEKLAKEAGVDLKRFEKDSK
                     YKRLEDINYLSTKYFYNQLKASTIAMKYVKERGFSDESIKNFGIGYTPKYPKLLDYIQ
                     KNTKYSKQELLQSGLFVEKEGKIREKFRDRIMFPIRSSNGKVIAFSGRVLPGNDWGPK
                     YMNSPETPLFHKKDNLFGQYESRQEIRKQDLAILCEGQTDVISAHQAGIKNIVAPLGT
                     GITKEQIEKLSKLTKNFLFFFDSDSAGFKAVVRAFELSSELGVYPYAATPSPYKDIDE
                     LLQKDKKLFAKKIKNRTEAFSFILSTYIEDKDLNKLTDVTIVKKFIDELLSNVKDPII
                     KSHYIEKVAKITTFTISKDFHGIRFSQECPSNKASLELPVKTLEERQELTYLKLLLLL
                     DKVPPKYIVKKSLLTSPQRQKVAELLSSNPGITRQEIYTALEEDPDTREVVEEAIFQT
                     TELPNNKEEIYNELDQIIQRLKKNFYENQRRDLHVKIAIAEEKGEFKQSQKLLSELQN
                     INKILKDI"
     gene            complement(4258..5193)
                     /locus_tag="PKI16_00820"
     CDS             complement(4258..5193)
                     /locus_tag="PKI16_00820"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNR53015.1"
                     /translation="MITAGEVLKSKRESLGKSLETVSEDTKIQIRFLRYIEDNDFSKF
                     DSDVFLTGFIKIYSQYLDLDSTKVLALFRRSKPTAKKNDLKTKSKSLFKVKKSFFTPK
                     FFITLSIALFLLLVIGYIGIQIYNFQSPPKLQITEPQNNLTTTSQYLTIKGITEENSS
                     LDINDSPVKINNDGTFEKELTLKEGVNLITVTVRKNSNTILESTEILKVTYTKEQEQI
                     VEKEEPQTNTLRLEVSNSVAWIKLDIDGQNKISQNVQPSKQNFTVERNFRVTTGRLAN
                     TKIFFNDAPISWNTNTTTGVAEISCEIINQLLNCK"
     gene            complement(5209..7431)
                     /locus_tag="PKI16_00825"
     CDS             complement(5209..7431)
                     /locus_tag="PKI16_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNR53016.1"
                     /translation="MPRPKKKEKLTIQSGETKILMGIILFVLGLAIGIASFMPLTSPL
                     FNQVTLKLGYSSIAWGIFLLYISFFLLTKSKKFKSWKQALGLFFFSASLSILLTFWQV
                     EEQLNNPDSLRSAGGEVGKILHLSLNNFVGDLIEIIIVLILLVIAFSLITGTTLEQIR
                     DFLESSGKKDKEDEKGFDLLSLFAKKDSLGDEKDVEIIEGEPEEDDQKRLDNAFSEGI
                     EPPIQPISPSISTEEDFDSAVRQVDSKVEDREPERPKYPNWEFPSLDILQDAEVQKQN
                     KQIYRDKAIVIEQTLKSFGIPAKVAQISVGPTVLRFALSISTGTKVSRVKNLSNDLAL
                     ALASQTSSVRVEAPIPGTSFVGVEIPNPTPNFVYIKEMIARLRKEPDKYELPLILGKD
                     ISGKTMIRDLGKIPHLLVAGATGTGKSVAINSIISGLLMTKTPDEVKFIMIDPKMGVE
                     MAAYNGIPHLLNPVITDMDLVVNALQWTINEMFKRYRQLKQAHTKKLSEYNKKMGYVA
                     MPYIVVVIDEMADLMLSCGADVEGKIQRLAQMGRAVGVHLILATQRPTVNVITGLIKA
                     NVPGRMAFAVATAMDSRVILDDTGAETLLGNGDMLFKDQSTPRPIRVQGTYTSTEDND
                     AVINAIKEKVQGKEIEYSEDLAQAIEKGPQTGDGTNREERDPDFPKALEIAILNKHIS
                     ASFLQRKMKIGYNKAARLIDQLYESGAIGPIDGVKPRQVLVSSASQILGKKNDDENVQ
                     "
     gene            7696..11082
                     /locus_tag="PKI16_00830"
     CDS             7696..11082
                     /locus_tag="PKI16_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNR53017.1"
                     /translation="MIKPQRNKAGRLDLGLNYSGGIEFPNLIESQIASFKDFYENGFQ
                     RLFADINPVKDNMEKMWTLEFKDFRYGQPYRTIDEAVTKGLSYEVPVYAKVQLLNNKT
                     GEIKEQEIFITDLPVMTDDGVFVFNGVRRVVTHQIVRAEGVLYEESDKLPTRTLHKVR
                     LMPGRGPWYEIDTNKHNVISMRILPKRPKVLITELLRVLGWETDDEIRKLFADVDTHN
                     EYKYIEATLARDFTKNKEEAIISIYNKLRPDESVTLESAEKYIKSNFFNVRKFDLGKI
                     GRYQLNRKLGTTCDINKPEEVMLYPKDLVLIVKRLIQINNGVVPADDIDHLCNRRIRS
                     VGEILERQLNAGVRRLEKNIKDKMSMYGQDAKVTPSMLVGTKPIAASIQSFFGANALS
                     TFMEQTNILSELENKRKVTAGGPGGISKDRATFSIREVHNSQYSKFDPVTSPESASIG
                     VVTQLAMLAKINEFGFLSAPYRKVLHEVQNSKTKLLNKVASEDIKSGKKVLVKAGTLL
                     TEELVKKVVEQKDIKNVKVKPFIDEEVVYFDAMEEEGHFISTATINTDEFGNIIDTIV
                     PVRHKGDFMLEDVNTVDYVDLLASQQAGLGMALVPFVSHNDAMRALVGANQQRQAVPL
                     VKQESPLIGTGLEEVVAKQSLWGIYAEDDGEVYYVDAKKMSVKYKKAGVKDYDLVSFY
                     RSNDNRCFSQKPVLNVGDKFKKDDILVDGPTMVNGELALGINLKAALMFYEGYNYEDS
                     VIISERIVRDDLLTSVHIREYTVEIRDTELGPEVITADIPHVNDRILQKLDAMGIVRN
                     GVRVKSGDILVGVVAPRGEKELTAEERLLRAIFGESASDVRDNSLRIPHGEDGIVIRS
                     QILSAEKGDKLSPGVLQEVKVWVANTKKINFGDKIAGRHGDKNTVAAIRPIEDMPFTE
                     DGEPVDLVLTPTFLKRMNMGQAQEIHYGRYAKELGEKLAFPIFEGKNDEWLKEELKKH
                     GLEMNEKITLYDGRTGKKFPRQIAVGIKYVLKLHHIADEKVHARSTGPYTAVSQQPLG
                     GKAQMGGQRFGEMEVWALEAHGAPYALQEMLTIKSDDVKGRSAAYKAIIHGEKIENVN
                     IPESFKVLSKEFNALCLNMDLISSKSSTEEEEVAQNEENI"
     gene            11066..14956
                     /gene="rpoC"
                     /locus_tag="PKI16_00835"
     CDS             11066..14956
                     /gene="rpoC"
                     /locus_tag="PKI16_00835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNR53018.1"
                     /translation="MKKIFNEKKNLNEVDILKLTLASPEQILNWSYGEVTKAETINYR
                     TFRAEPDGLFCEKIFGPTKNYECYCGKYKKIRYKGIVCDKCGVEVTTQDVRRERMGHI
                     ELCVPVAHVWFAYGIPNKMSIVLNISHKKLLSVIYYTRYMVVSVEKNRKAGIIEKANA
                     QKEEEVTSLNSQLEEELKMTSEMYDDQIKEVKKNKGKNTEFKISQIEHSRKQGLAKIR
                     REFAEKEEELESAYTKILLVIEKLKVGTVLSEDEYIELEERDLLFFEAMMGAEAIKKL
                     LESLDLEKEIGKLKKRAEKEKGERKLATIRRIQYLEGFKKNNLNPGWMIITTLPVLPP
                     ELRPIIPLSGGKFATSDLNDLYRRIINRNNRLRRLIEIGAPDVILRNEKRMLQESVDA
                     LIDNSHRPSKAMMNNKRLPYQSLTDDLRGKKGIFRKNLLGKRVDYSGRAVIDGDPSLK
                     FDQCGLPKSVALEMFKPFVIQQLLIREIAPNVRMAKEMIEEEEDLIWDILEEVIKNKP
                     VLLNRAPTLHKYSVQGFYPKLVEGEAIRIHPMVCKAFNADFDGDQMAVHILLTDEARK
                     EAEREMMSSKSIVNISNGQVLASPAKDMLLGFYLMTDFEPKKNPRIYESVESAVKAYE
                     RDLIDIKEEIIVRAKGERLNTSVGRVIFNSILPEDYKFLNDRVNVKTVMRIIDDIKNS
                     RPMEDVVELLDNMKLLGFKYATDLGFSFSMGDCNVDFDMVSELKKIEEKDEQLQENYL
                     QGLMTKNEKINISTNMWDDFSNELAEKAWQKLEKNNPVFEMVESGANGGKIQARQVLT
                     IKGVVRDSRGNLVPMPIKSNYRDGLSAFEYFVAANGGRKGIADRSLKTSSSGYMTRKL
                     VDVAHDVIIRKDDCGYEGEGLVLKRDDDRRMDFVDRIFGRVPARDIKIGKKVVAKKNE
                     AITKEIALEIDKSDLSEISVRSPMLCDSPIGICKKCYGLNLENGQEIEMGKAVGVIAA
                     QSIGEPGTQMTMQTFHKGGVAKVDITQGLPRIEELFEARTPKAEADIASIDGKVHVEI
                     AEDESATISISGEKKSTRFYVVSKAKKVLVENEQEVKAGQIIFIDAEENEKQAPFDGK
                     VTLTGGIMGINGTIKAEEIVSVLPGVSILVEDGETIKAGQQLVEGSVDPKKLSIVADI
                     ITAQKYILDEVQKVFNEQGVPIDDVHLEIILRQMARLGKVEESGDTDSLVGSLVNRFL
                     ADAKNRLLLSQGKNIALVTPKLFGIKTSSLNTEGFLSAMSFQEQVRVLTSSAILGKTD
                     FLRGMKENVIIGRLIPVGERAEIKDINDVAELKF"
     gene            14982..15401
                     /gene="rpsL"
                     /locus_tag="PKI16_00840"
     CDS             14982..15401
                     /gene="rpsL"
                     /locus_tag="PKI16_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNR53019.1"
                     /translation="MPTINQLIRKPRKSKAKRVKHIALATNFNPLKNRYSREKNPFKR
                     GVCLQVKTMTPKKPNSALRKVARVRLSNGMEITAYIPGEGHNLQEHSVVLLKAGRKRD
                     LPGVKYIVVRGKYDTAGVQGRKKARSKYGAKSPKNAD"
     gene            15423..15893
                     /gene="rpsG"
                     /locus_tag="PKI16_00845"
     CDS             15423..15893
                     /gene="rpsG"
                     /locus_tag="PKI16_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNR53020.1"
                     /translation="MRGKRAKRREQVGDRKYGNLTISRLINKVMQDGKKTIAEDLVYT
                     ALNSAAESAKEKDIAIFMNRVIDNVKPALEIRARRVGGANYQVPVPVSPRRQETLAIR
                     WIVDTSRAKSGTSFDKTLATELLNAYNGEGEAIKKKQDTERMAEANKAFAHFKW"
     gene            complement(15968..16909)
                     /locus_tag="PKI16_00850"
     CDS             complement(15968..16909)
                     /locus_tag="PKI16_00850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR53021.1"
                     /translation="MISVIITSFKEPHTIGKCIQCIADKKYSGIIKPFEIIQVSPDEL
                     TLKAGKKMANILKLSNSEYHHIIDPHKGKPYALKLALKKAKGDIIILTDGDTFFGENA
                     VAELIKPFENAKIGGVSGRPVSQDSRENIFGYWGHLLSESGDHRRRNTMQEVRRNGYY
                     VSGKSFYPMSGYIMAIRNIDLKIPDNVLSDDAYISYSLRNDGYEIAYNPNATCYVKYP
                     TNLKDYYKQKVRSLGGFKQLKKFGIFKKDKQSRSFFIELPYAFFVLKYPRNVKEFVWS
                     LLLFPVRLITWIKIFWERDILRKDMPSTGWERIESTK"
     gene            complement(16949..17608)
                     /locus_tag="PKI16_00855"
     CDS             complement(16949..17608)
                     /locus_tag="PKI16_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNR53022.1"
                     /translation="MEKIVFTKRNILGKKSKNLLKENLTPAVIYNAKGESTNIQIDSR
                     IAGQISREATSTTIYDAELDGKASKVLVKEIDFNPMTEELRHISFFEIDESKPMVFTI
                     PFHIVGISPAVKNNLGVLVEALDSIDVRCKLSDLKPFIEIDISGLDHPGQSIAVEDIK
                     LPEGMSLINEDTKKSTIVTVTEPQKEEEVIAPVAAEGEEVAEGTEVTEEGAETPTEEP
                     K"
     gene            17721..18446
                     /locus_tag="PKI16_00860"
     CDS             17721..18446
                     /locus_tag="PKI16_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53023.1"
                     /translation="MNITERQNAILMAVIKEFMDDANEVGSLSLVEKYNLGVSSATIR
                     NEMVKLMSLGLLEKNHISSGRLPTDQALRLYVSKIIDRSSLNPLVSVEIRQGIFRDRF
                     SKDIVIKSILQILAKETDSVVFLILDDDVRYYGLANILAYEEFQSIEKIKSVISILED
                     DVFLKNMAQKYSSSGVSLLIGEESGVGTLQDCAMAFTKIPFWNKSNSYVGVIGSKRMD
                     YSKVIPALREVKNSLENAMSGWR"
     gene            18463..18957
                     /gene="grpE"
                     /locus_tag="PKI16_00865"
     CDS             18463..18957
                     /gene="grpE"
                     /locus_tag="PKI16_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNR53024.1"
                     /translation="MKKELESKKIKDLALIIDKLEDENLVIKEQMLRALADYQNLVKS
                     SEKRNEIRYFQLKKSLSESVIPSLDALNLAIKASKELKLDEKTKAWLDGILATIQSLE
                     KSFSEIGIKQYLPIKGDKFDPTKHEAVATVEGGKSGEIYDLVQPGYILDNTVIKPARV
                     VVSK"
     gene            18982..20874
                     /gene="dnaK"
                     /locus_tag="PKI16_00870"
     CDS             18982..20874
                     /gene="dnaK"
                     /locus_tag="PKI16_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNR53025.1"
                     /translation="MSKIIGIDLGTTNSVMAYMEGGKPNVIPNAQGGRLTPSVVAIGD
                     KGEILVGNPAKNQAVTNPEGTIYSVKRLIGRSWDDPEVKKDKELLPFEMRKSSSGGVE
                     VKMKDKWYSPQEISAKILAKMKKDAEDFLGEEVKEAVITVPAYFDDSQRQATKDAGKI
                     AGLDVKRIVNEPTAAALAYGVEKGKEEKVAIFDLGGGTFDISILEIGDGVFEVLSTNG
                     DTHLGGDDFDQKIIDFLADQFKAKEGIDLRNDRTSLQRLREFAEKAKVELSTSEETEI
                     NIPYITADANGPKHLKEKLTRSELEKLTSDLIERTVEPCKKALKDAGLSIKDVDEILL
                     VGGMTRMPAVIKKVEELFGKEANKGVNPDEVVAVGAAIQGGVLGGDVKDITLLDVTPL
                     SLGLETLGGIMTKLIEKNTTIPVEKKQIFSTAADNQPGVEIHILQGEREMAADNKTLG
                     RFILDGIPPAPRGVPQIEVSFSIDANGILNVKAVDLATKKEQKITITASTGLKEEEIE
                     KMVEDAKKNAEEDKKKKERAETRNEADTLCFGVEKLIKENGDKIDSSEKEDLEKQSEE
                     LKKIIEKEDFDVEDVKKKTDELMEKMQEVGKKMYEKVAQESKPEEERKKEEKKDDDVK
                     EGEVVD"
     gene            20969..23308
                     /locus_tag="PKI16_00875"
     CDS             20969..23308
                     /locus_tag="PKI16_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53026.1"
                     /translation="MLRDSEGTKKEKIALPVIQKYLFLAMLFLLPLSIIPFPWDWTEK
                     GMSLVILIFSTIIIGIEIIKLIWDGKATLIKSLLDMGSFAILISLILSTLLSKDINSS
                     LWGVDNRLGSGLIVFIAVILVSFSARTFIHNMRDIKMVILSFLGGLFISNILSIFSFL
                     GSNIWEIIPVYKEMHQAGLPLLRSEKVQVLINMVGILLSLGIIGERMLRQEGKSIAMT
                     LCYLFLVPAIVNLWVFSIHQGLAILVLFTIILILILILVLPRLKIGRGLKRDLVVLFI
                     GIFLTIALPVILLQIPSLRAIIIPRGVNLVAEVSLGADISWIIAASVFVDSLWKGLFG
                     LGVDTYTIAYNMFKPLNEALISFNSVNFYFAGNELFTKFSNGGLIWLIAWGFFGFLLA
                     KLLKDDLRRFRLYQEDNAAFLLVMFDFTLVFLFLSSLFVTYSIYVIFVLFVLISLHSI
                     LKDILGKGSGDKFVFKLWAVNVSSKVESGKAMYNINVFLTVVTVLITCGFLVLWGSKV
                     ISSAYLLKAESYYIEQKKIYAEKEPSLNQREDFVKRMTGYYSKALKFDNKDPLVNRKL
                     GLMYLEQVGIAAEKYSKNKESEEANTIIKNIGQWKNYAIDYIRKSIDRSPAIYANWEA
                     RARVYMGLVGFGFYDYTADALFSLEKAIELNPLNFELYYYKAQLNVISGDKDGALSAL
                     TQVLGINPQHVASIMLAGEINKEKGNIEVYESYLKAAKKILEIQGNTDIEQYKEISNQ
                     LNELAKDDSKGEVKEEETAEEPTEETTPVVETVPAEAGE"
     gene            24094..25884
                     /locus_tag="PKI16_00880"
     CDS             24094..25884
                     /locus_tag="PKI16_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNR53027.1"
                     /translation="MIKLGLKKNSIFFVMFLLFITKILGFLKLRTIAQLFGVSHELDI
                     FWAAFTIPDMIFTVLVAGSINAAIIPIFSEIFYKKGKESLDRFFNYLFIVLTVSCLLI
                     AGLFFVFTPWITNSVINSEVLQKFLDLSGRITPADYHMFVTLTRLMLLSPILLATSSL
                     VTAYLQIKKEFLTTSLAPIFYNLGMIIGPIIFVVFGNLGVEGIAISAVISSVLHLLVQ
                     LPQFAKHFNDGFSLSPSYFKNAFQDIHIWRTFKLAIPRTIGILGEQINGLVNTLISFS
                     LTAGALSAYRFAISLHLFPINIIGSAVAQVALPDLAQHSGDRKKFVKILDSSIQFALY
                     IVFPIVAMMMILRLPIVRLVYGSGAFDWRATILTAWCLVLLGFSIIGQTVAQIVLRAF
                     YALKETWLPLVAIVISIIVNIVLAYYLTNFFSHYYDWRPIFDQMVYQISSANGEGFLV
                     VLKSFVKDFLRWSSDRGTSDMAVGGLALGFGISYFVEMILLMLLLNFKGKIITWKRTI
                     KPILVKILNTVIMAIGMYFVFKLFDFQLDTTRTWSIIVLSAAVCSYGLLSYWIGSKIF
                     RIKEVDKFESRIVVIFSKFFSRGEKDAETV"
     gene            25868..26461
                     /locus_tag="PKI16_00885"
     CDS             25868..26461
                     /locus_tag="PKI16_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53028.1"
                     /translation="MQRLFKIFGIFLLLLIFLPIFLTGMYLLWEREFSLNLSSKSCVS
                     NNQVKEISFDERIRNFVFSSDRSDYIQFTKEEVLYLISSEFGKEEGDIVVKDMCVDSS
                     KGVWKVYLNLTVAGIKNVWFIFDIIKDDRETAELYVDNLYLGNFQLPSFISSSLKEKI
                     NKGISDAIVLVNENSFSGRSITNIELLEEDIVVKGVY"
     gene            26528..26809
                     /locus_tag="PKI16_00890"
     CDS             26528..26809
                     /locus_tag="PKI16_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNR53029.1"
                     /translation="MSKKINIMPLGKRILAKPDAIEEKTAGGLVLPPNVNDEQRSATG
                     TVVALGKAKSFDVAIGDRIYFKKYSPEEIEIEGVKYLILDTDDVLAVIK"
     gene            26828..28450
                     /gene="groL"
                     /locus_tag="PKI16_00895"
     CDS             26828..28450
                     /gene="groL"
                     /locus_tag="PKI16_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743854.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNR53030.1"
                     /translation="MAKAIIYNEEARKALKEGVDTISNVVKTTLGPRGRNIAIAKSYG
                     SQIITKDGVTVAKEIEDRDPFKNVGVEMIKEASSKVNDLAGDGTTTVAVLAQAIANLG
                     FRNVSAGSNPIAVKRGIDKGVEMIVKELEKKAKKISGLEEISQVATISANNDKELGDM
                     IGGVFEKVGKDGVITVEEAKGFDDEVEYTEGMEFDNGYVSAYFVNNAETLETVMDNPY
                     IFITDKKLSNLQDIQAIAEKVLGAADRPLVIIAEDIENQALATLVVNKLRGTLDVVAV
                     KAPGFGDRKKEMLKDICVLTDAKFISEELGNTLDKVEITDLGSAKKVIVTKEKTVIVE
                     GKGSKKSINDRVSEIKAQISKTSSDYDKEKLQERLAKLVGGVAVIKVGAASETEAKEK
                     KMRIEDAINATRAALEEGVVAGGGLALHNAKSVLNDVKLEDVDEQVGIDILKSILSEP
                     LKQIAENAGEDGAVVAANCSGKLGFNAKTGEYVDMIEAGITDPVKVTRLALVNAASVG
                     TMLITTEAVVAEIPEEKSKAPMSPDMGMGGMM"
     gene            complement(28502..28957)
                     /locus_tag="PKI16_00900"
     CDS             complement(28502..28957)
                     /locus_tag="PKI16_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53031.1"
                     /translation="MRNREFLENLMYELWENHFCDIPRKNMVVIKFGKYSKRQLGSIK
                     LANGRTKIKGLIKNRKDDFLTQDEKSITVITVTRYFQNEIVPEDIVRATIAHEMCHYA
                     HGFSSPLEKQFNNPHKGKIIDRELKKRGLEQLQKDTDTWLKENWVKIVY"
     gene            28992..30800
                     /locus_tag="PKI16_00905"
     CDS             28992..30800
                     /locus_tag="PKI16_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR53032.1"
                     /translation="MILQKYKKKISTLKKSLSFVYGRYTTAAISRDILFAISTASELY
                     SITLLGKFIDETAKILLSWESFNLTEYLGTKSFLYLVLILALWGVTQICVQAREYFYN
                     VIYERTWEDAKYMMMAKVSRSNLQDVEKEEFQDYLTYAPTFSIDRLITAYSDFSVILS
                     NIIRLIGALVIIWGVAGWWSLILVILVFPEVIAVHLRRREIKKEQDNAVGKFKFLNYI
                     QGVALQISNFCELRVNDTFSFLKRRYTEEYDDFLEGYLKKQANFYQDKTIFSIIDQVF
                     KFAFVILLLAFSIAQRLTIGTFKALYDYVDVAYNSIYSIMNTVSLLANKLEYIDEFFD
                     LIEYEGFGDHQHGNVKLPKGTPLLEFTNLDFAYPDDPDVKILKDLNLVVKPGEKVAFF
                     GGDGSGKSTTVKILSGLYQVVSGDFQIGGHSVRELDRGELKKKIAVTFQDFVNYNFSV
                     LENIVISDKRKKIDEELYKKVYTISKVKDFVKKQKIKESHLLGKTFPGGMDLSPGYWQ
                     RLAIARMLYRNKNIFIMDEPFTFIDSISASEILKGIFEFLGKERSLIFITRSTENLKM
                     FDRIYYFEDGKIVEEGTWRSLMKRKGKLYHEVSSLN"
     gene            30827..31705
                     /locus_tag="PKI16_00910"
     CDS             30827..31705
                     /locus_tag="PKI16_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNR53033.1"
                     /translation="MEILDGKKISEEILSNISDEVERLRVQNKRIPRLDMIIVGDDYG
                     SIKYVGMKEKTAREVGIEGQVHHLPFSSNTLDIVNLIDSLNRFDGVDGFMVQLPLPEH
                     IDTQLVLNTIAPQKDVDGLTAINLGKLFQKDPSAIPPATPLGIMKLLDVYGIKLFGKH
                     VVILGTSNIIGTPLAAMMLEKNATVSLCNAHTINIQEISSNADILVSATGKALLIKKD
                     FLKPGCVVIDVGSNKHPETGKLVGDVDWEDVKGIPSYITPVPGGVGPMTISCLMLNLM
                     SAYKKNDSRQENTRTH"
     gene            31671..32882
                     /gene="glyA"
                     /locus_tag="PKI16_00915"
     CDS             31671..32882
                     /gene="glyA"
                     /locus_tag="PKI16_00915"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNR53034.1"
                     /translation="MIQDKKILELINDEKKRQREGIELIASENYVSESVLECMGSILT
                     NKYSEGYSRKRYYKGNEYIDDIEEEAISRAKELFHAEHVNVQPYSGSPANLAVHFALL
                     DVGDRTLGMALDAGGHLTHGFKVSITGKYYNSVSYGVDEHGYIDYEEVRRLAREHMPK
                     LIWAGFSAYSRIIDWQRFRDIADEVGAYLAVDIAHIAGLVATGIYPSPVGIADVVTTT
                     THKTLRGPRGAMIMCKEELAEKIDKAVFPGLQGGPHNHTTAAIAVALKEASTEEFKEY
                     TKQIVKNSKALAEELIKRGYKIISGGTDNHLFLVDIVASVGISGEEASSLLEQSNITV
                     NKNAIPNDTRKPWDPSGIRIGTPAVTTRGMKEKEMVKIAEFIDEVLRNRKNVRAEVKD
                     FTKDFHIPGIN"
     gene            32894..34489
                     /locus_tag="PKI16_00920"
     CDS             32894..34489
                     /locus_tag="PKI16_00920"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNR53035.1"
                     /translation="MKLTYRKDLNIATIRSESDWNKFKVHTLLQNPTQKQPSINAYLG
                     ARYSRSADSITDIASEIMESGTDAAEKLEKIFSGYGHKSVGDMADLFLCIENIPMYTA
                     MRIYYMCSVLSGQERSTRYQNFEKPEFIKIPKEVCANYEVRKEYERIILKQMQDYREM
                     MKPTREALEKAFKINEESPQEVSALKARSFDVCRYFIPLGIHTSSAYLMSARNWSELI
                     SLLCANDSVVENDVADLILNLLGDSKLEVKGYLKEADNLIRHTDANCCRKNSSKAILE
                     YLKERISREQVNDIPESEIDKLEIAHGSDSTEALISNYELLLNPLGSKQEFEFTDFDQ
                     ESIGEIIFDNHDHHNLLGNIGQFGNIKITGFASIGTLKDLNRHRSLERFIPFFHDDID
                     LDQELNRPNFKCFFLCNYLDIKSISKLRGEYEKRLTETYKIIKEWRKKAKGNMTKEVC
                     DEFTKYLLPHAHSTRYVFYGSFDDLQYTINLRTRNGGHIAYRVLTYEWLRQLSLADPI
                     WRPLLKRIVAPKIDDKHQFVDRS"
     gene            34507..35094
                     /gene="dcd"
                     /locus_tag="PKI16_00925"
     CDS             34507..35094
                     /gene="dcd"
                     /locus_tag="PKI16_00925"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNR53036.1"
                     /translation="MILSDKSIKAKVKTGEIVIEPFEERCLQSASYDLHLDKDFLVFN
                     RENHSLIDVKEPVDELMKKIVVSEEQGIIIHPGDFILANVKEITGVDDKHVGRLEGKS
                     SLARMGLIIHTTAGFLDPGNKLRLTLEMVNLSPLPIKLYPGMKIGQIAFEELDEKCER
                     PYGTKGLMNKYVGDMTVTASQMWKNFKEEDNEQKD"
     gene            35078..36358
                     /locus_tag="PKI16_00930"
     CDS             35078..36358
                     /locus_tag="PKI16_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNR53037.1"
                     /translation="MNKKIRNQFPILQRKINGKKLIYLDSAATSQKPQFVIDSISEYY
                     KKYNANIHRSSHVLAKEATDMWKEAHQIVADFLNAESYKEIVFVRNSTEGLNLLAKTY
                     GKDNLSKEDIVIISEMEHHSNIVPWLILQKEIGFEIKYIPVTDDYDLDIKWLEDTVKE
                     FGSKIKIISVVHISNVLGTKNDVKKISEIGHTVGAFVIVDGAQSVPHIPVDVQDIGCD
                     AFVFSGHKVYGPTGSGVVYCKQNILEKLSPFMGGGEMILSVKKDGFEMNELPWRFEAG
                     TPDIESGIVLGKTIQWFKKTIEEIGGWDVLIEHEKNLMKEFLDSFDGIEWFRLFGKKS
                     VEERYGVISFNIDGFGFKGCKEMEKGKKGGSEIVEYISARGVAVREGYHCAEPLHDRF
                     KVGPTMRMSLGIYNDNEDVKTFSKLLKEAVLSSY"
     gene            complement(36355..>36908)
                     /locus_tag="PKI16_00935"
     CDS             complement(36355..>36908)
                     /locus_tag="PKI16_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53038.1"
                     /translation="PEEVVTQVEEKKEETPAPAESRPTDIDNISENLYTPQVKEETIQ
                     TKLDEQPTQLQSAEATESNDLADLMAKDNTQVQATPAPNLNTMPVETPTPAVEETTPT
                     PNFNTIPVETPAPVAEETTPTPGFNAMPVETPAPVVEETAPVLPSNLPDMQQMVNNGA
                     VAPKEETTPTEEPQSVPPVQPTI"
BASE COUNT        12618 a         5570 c         7281 g        11439 t
ORIGIN      
        1 ttaatattgg tatcgtaaaa ctatcaagtt taaaaaatat taatatagcg gcaaagatag
       61 atagggaaag gaccaatcca cccagagagg gtgtaatccc ttcgtgcata gctttttgtg
      121 gattatcaaa atccttttca tttcttttcg aaaaaggctt gtaggtaatg tcatattttt
      181 gagcaaaatg tcctattagt ggtgttaata taagagaaat tacaatacca ataagtagaa
      241 taggccagaa atcaaagtat ttcgaatatg agtcactagt aattaaatct gaaaaatgtg
      301 taaggagctt ttgtagtagg atgtttgaag aggaaaaatc cattagaatg cattaattaa
      361 ttgaattaga gatattgtca tagatacaca acaaagtaat gcagacagga ggagcaattt
      421 ggttaaaaac ttctctctgt tgtagactct aatagttaga aaggtcgctg tagcaaggga
      481 aactagagat atcactggat atattacaag atatattttt gaagttaatt tcgcagatgc
      541 agcgatattg tatctaagaa ttggaagcaa agagggaagg ctgtttagat ttaccactat
      601 tatatatacc tggtaagcaa tcattagaag tgaaattacc aaaacaaacc agaagaatat
      661 atcttttagg aactttctaa cctccagttg tattaaaaga gagtctgttt ttccttttat
      721 tttaaagaat ttgtttttaa aaggctcctt tatctttagt cctagttgag tgttttgatc
      781 ctcctttttt cgtgtagaag aaattttctt ctttttcata ttattgatta taccagaaca
      841 gagctcttgt cgttaaagag ttgcagatat tcttccaggg agagatgttg aggtcgtttt
      901 tgtaataatt tgtcaaaatc actactaaat ttgtattctc ttaggttgtt gtgaattgtt
      961 tttcttggat gtgtaaagca ttgttcgagg aaagaattaa aagagggaat atctatgggg
     1021 agagtttttg tggtaggaga gaacattata agggagctgt ttacttttgg ggatggttta
     1081 aaggaggatg gtccaatatc aaggattttc ttacaatttg cataaatttg ggttctgagt
     1141 gaaagaagat tagaatctgg tacttttgcg atatattttt ctgcgacttc tttttggact
     1201 atgaaataac agttgttagt aaagtattgt gaagatatta tttttgaaat aatttgttta
     1261 ctaacattgt aaggaagaga tccgaagaat ataacttgct tatcgtttaa ctctttaaaa
     1321 tcccaatcta ggaaatctac atttaaaaca tttattcctt gtgcaactaa cttttcagcc
     1381 aaaacattat ctttctctat tgctagaaga ttcttgccca tttcttttag ttttattgta
     1441 aaaaaaccaa tcccaggacc tatctctaca acatattggc atgactcatt ggaaatatat
     1501 gaaatgattt ggtttccttg attttcgttt atgaagaaat tttgtcctaa agatttctta
     1561 aattccattt atttaattct aaaatcccaa atatcgttct ctttaacaaa ggttaaggaa
     1621 cactttttgc acttagcttc actgtttaca attgaaagga ttcctttaca ttttggacaa
     1681 gctaaaatat cttcaagttc agatgtatgg actacatttt cttttatttc ttttttaata
     1741 atactctcaa atacaatact tggaggaata ttactccaag agagtaaagg ttgaagaatt
     1801 ctttctgtta ggatcattaa ctcctccccg aaaacttttt tgagtaaagc agaacgaata
     1861 tatgaacagc cgtgcttttt aattactgta aaacccttct ttgttagtaa ttctttaata
     1921 tatttgggat ggtagttaaa aaatatagcc tcttctccgg attttgttcc ttcgaaatta
     1981 ccttttgttg gttgttggta tggttctttt gaaaaaaact taaaatttaa tctaacaagt
     2041 gcttttaatg tagaaatgag atgtactttg tttgcaaatt cctgtatgta aaaggaattc
     2101 ctattcatca cacggtgtaa ctcttcaaaa tatgctgagg gtttctcaat gtggtgtagg
     2161 actcttacca ttagtgctcc atcaaatgta ttatccttga agggtaattt gtatgcattt
     2221 gcagctatta attggatatt ggggtatttt gtttttaaaa tatctttgtt tttgaccaat
     2281 gtttgaagtg aatagtcaag gattattgga tttgtgtatt ttttgtaata tgtagaactt
     2341 aatctaccgt aagaacctcc gatgtcaaga aaccaattac cattcttgtt agaaaaaatc
     2401 tttgttagaa gtattttttc tgcttcattt tcatatcttc tgttactcca ataattagca
     2461 taatcatatc caaagaaatc gtaatctgtt actttagtca tcttaaatat cttttaatat
     2521 tttatttata ttttgtaatt cactaagtag cttctgggac tgtttaaatt ctcccttttc
     2581 ttctgcaata gctattttta catgtaaatc tctcctttga ttctcgtaga agttcttctt
     2641 tagtctttga atgatttgat ctagttcatt atatatttct tccttattgt ttggtaactc
     2701 tgttgtctga aatatagcct cttctacgac ttctcttgta tctgggtctt cttcaagtgc
     2761 ggtatatatc tcctgacgag tgatgcctgg attggaagaa agtagttccg ctactttctg
     2821 tctttgaggt gatgtcagca agctcttttt aacaatgtat ttggggggca ctttatctag
     2881 taacaacagc agttttagat atgtcaattc ttgccgttct tccaacgttt tcacgggaag
     2941 ttcaagggaa gccttattac taggacattc ctgtgaaaac cgaattccgt gaaaatcttt
     3001 tgaaatggta aatgttgtaa tctttgctac tttttctatg tagtgagatt taataatcgg
     3061 gtcttttaca ttagaaagta actcgtcgat aaatttcttt acaatggtta catcagttaa
     3121 tttattaagg tctttatctt ctatataggt tgagagaatg aacgaaaaag cttctgttct
     3181 gttctttatt ttctttgcga aaagcttctt atctttttgc agtaattcat ctatatcctt
     3241 gtatggggaa ggagttgcag cgtatggata tactccaagt tcagaagaga gctcaaatgc
     3301 tcttactaca gctttaaatc ctgcactatc actgtcaaag aagaaaagga agttttttgt
     3361 tagcttagaa agtttctcta tttgttcttt tgttattcct gttccaagtg gtgcgacaat
     3421 gtttttaatt cctgcttgat gtgcagatat gacatctgtt tgaccttcgc aaagtattgc
     3481 taggtcttgt ttcctaattt cttgtctaga ctcatattga ccaaaaagat tgtctttttt
     3541 atggaaaaga ggtgtttctg gggaattcat atatttaggt ccccaatcat ttccaggaag
     3601 tactcttcca gagaatgcga ttacttttcc gttagaggat cttattggaa acattatcct
     3661 gtcacggaac ttttctctta tctttccctc cttttcaaca aaaaggcctg attgaaggag
     3721 ttcttgtttg gaatattttg tgttcttctg tatgtaatct agtagttttg gatatttagg
     3781 tgtgtaacct ataccgaaat tctttattga ttcatcacta aaccctcttt ctttaacata
     3841 tttcatagca attgtggaag cttttagttg gttgtagaaa tattttgttg agagatagtt
     3901 gatatcttca agtctcttgt atttactatc tttttcgaac cgtttgagat caactcctgc
     3961 ttcttttgct aatttttcaa gggcttctac aaagtcaatg ttttctatct tttgaacaaa
     4021 gttaaagata tctcctgttt ctccacatcc aaagcattta tatctttgaa tatctggact
     4081 tacaatgaag gaaggagttt tctccttatg gaatggacat agtccagaga agttcttgcc
     4141 agcttgtttt agttggacat acttacctac aacctgaact atatccaaac ggttcttaat
     4201 ctcttctatc tgattatttt gatccatact ttagctgatt atatcatcaa aagtggatta
     4261 tttgcagttt aataattggt tgataatctc acaagatatt tctgctactc ctgttgttgt
     4321 gtttgtattc caggagattg gtgcatcatt aaagaaaatt tttgtatttg caagtcttcc
     4381 agtggtcact cgaaaatttc tttctacagt aaaattctgt tttgatggtt gaacattttg
     4441 tgaaatttta ttttgtccat ctatatctag ttttatccat gctactgagt ttgagacctc
     4501 aagtcgtagg gtgtttgttt gtggctcctc tttttccact atttgttcct gttcttttgt
     4561 gtatgtaact ttaagaattt ctgttgattc tagaattgta ttactgtttt ttcttacagt
     4621 aacagttatt aagttaacac cttccttaag ggttagctct ttttcaaaag ttccatcatt
     4681 atttattttt actggagaat cattgatatc taaagaggag ttttcctctg ttataccttt
     4741 tatagttaaa tattgtgatg ttgttgtaag attgttctgt ggttctgtta tttgtagctt
     4801 aggaggactc tgaaagttat atatctgtat gcctatgtat ccaatgacaa gtagaagaaa
     4861 gagtgcaatt gaaagggtta tgaaaaactt tggtgtaaag aaagactttt taactttaaa
     4921 aagagattta ctttttgttt ttagatcatt tttctttgca gttggtttac ttctcctaaa
     4981 aagagcaagc acttttgtgg aatctaaatc tagatattgg gaatatattt ttatgaaacc
     5041 agtaaggaat acatcggaat caaactttga aaagtcatta tcttctatat atcgcagaaa
     5101 acgaatttga atctttgtgt cttctgatac ggtttctaag cttttaccta gggattctct
     5161 tttagatttt aatacttctc ctgctgtaat catgaccaat tctactactt attgaacatt
     5221 ctcgtcatca tttttcttcc caagtatctg cgaagcagaa gaaaccaata cttgtctcgg
     5281 ttttactcca tctatcggtc ctattgcacc tgactcgtaa agctggtcta tgagtctcgc
     5341 ggctttgttg tatccgattt tcatttttct ttgtaggaaa gatgctgaga tatgtttgtt
     5401 aagtattgct atctcaagag cttttggaaa atcaggatct ctttcttctc tgtttgtacc
     5461 atcacctgtt tgtggacctt tttcaatggc ttgtgctaaa tcttctgagt attcaatctc
     5521 tttcccttga actttctctt ttattgcatt tatgaccgca tcattgtctt ctgtagaagt
     5581 atatgttcct tggactctaa tgggtcttgg tgtcgattga tctttgaaaa gcatatctcc
     5641 atttccaagg agtgtctctg cacctgtatc gtccaagatt actctagaat ccatagctgt
     5701 tgctacagca aatgccattc ttcctggaac atttgctttg atgagaccgg ttattacatt
     5761 gactgtaggt ctttgagttg caagtatgag gtgaactcct acagctcttc ccatctgtgc
     5821 aagtctttgg attttacctt ctacatctgc accacaggag agcatcaggt ctgccatttc
     5881 gtctattact actactatat acggcatagc tacatacccc attttcttgt tgtattcgga
     5941 aagtttttta gtatgggcct gttttagctg cctgtatctc ttaaacattt cattgattgt
     6001 ccattgaagt gcatttacta ccaaatccat atctgtaatt actggattta gtaaatgtgg
     6061 aataccattg tatgctgcca tttctacacc cattttaggg tcaatcatta tgaatttgac
     6121 ctcatctgga gtttttgtca tgagtaagcc agaaattatg gagtttatag ctacagattt
     6181 tcctgtacct gtagcacctg caactaagag gtgtggaatt ttacctaaat ctcgtatcat
     6241 tgtttttcca gaaatatcct ttccaaggat taaaggtagc tcatacttat ctggttcttt
     6301 tctcagtctt gcaatcattt ctttaatgta aacgaagttt ggtgttggat ttggaatctc
     6361 gactccgaca aaggaagttc ctggaattgg agcttcaact cttacggaag atgtctgaga
     6421 tgctaaggct agtgctaaat cgttacttag atttttgact ctagatacct ttgttccagt
     6481 actaatactt aaagcgaacc ttaaaacagt aggtcctacg gatatttgag ctacttttgc
     6541 aggaattccg aagctcttca aggtttgttc tataactatg gccttatctc tgtaaatctg
     6601 tttgttctgt ttttggacct ctgcatcttg gagtatatct agcgaaggaa actcccagtt
     6661 tggatatttt ggcctctcag gttctctatc ttccaccttt gaatctacct gtctaactgc
     6721 actgtcaaaa tcttcttcag tagagatgct cggagagatt ggttgaattg gaggctcgat
     6781 accctctgag aaagcgttat ccagtcgttt ttggtcatcc tcctctggtt ctccctctat
     6841 tatctccaca tccttttcat ctccaagaga atctttcttt gcaaaaagag aaagaaggtc
     6901 gaaacctttt tcatcttctt tatctttctt tccagaggat tcgagaaaat ctcttatctg
     6961 ttccaatgta gttcctgtaa ttaaggagaa ggctattacc aaaagaatca agactattat
     7021 tatctctatg aggtcaccaa cgaaattatt aaggctcaag tgtagtattt taccaacttc
     7081 tccaccggcg cttcttagcg aatctggatt atttaactgt tcctctactt gccaaaatgt
     7141 aagaagaata ctaagagaag cagaaaagaa gaagagccca agagcctgtt tccaagattt
     7201 aaacttcttg ctttttgtta aaaggaaaaa agagatatat agtaggaaga taccccatgc
     7261 aattgaggag tatccgagtt ttagagttac ttgattgaaa aggggtgatg taagaggcat
     7321 aaatgaggct ataccaattg ctaaacctaa aacaaacagg attattccca ttaatatttt
     7381 tgtttcgcca ctctgaatag taagtttttc ttttttcttt ggtctgggca tttgtttatt
     7441 atataataaa taataagata ataagaagtt ttttacaaag actaaaatat aatatataaa
     7501 tctggttcaa aggtctagtg caaataatat attaattatg ctaaaaacct ttgactaata
     7561 gtagtaaata tgataacatc cttcgagttc cgttgttttt gcttacaatg tgatgttttt
     7621 tatatggctc gcatgagtca taaacataaa agcttgtaag aataatgtta taacattaag
     7681 tataaatatc gctttatgat taaacctcaa cgcaataagg ccggaagatt agatttggga
     7741 ttgaactact ccggtgggat tgaatttcct aacttgattg aatctcagat cgcatcattt
     7801 aaagatttct atgagaatgg tttccagaga ctttttgcag atattaatcc tgttaaggat
     7861 aatatggaga aaatgtggac tttggaattt aaagatttta gatacggtca gccatacaga
     7921 actatcgacg aagcagtaac aaagggtctt tcctatgaag ttccagtata tgctaaggta
     7981 cagctcctaa ataacaaaac aggagagatt aaagagcagg aaatattcat aacagatctt
     8041 ccggtaatga cagatgatgg agtatttgtt tttaatggag taagaagagt tgtaacacat
     8101 cagattgtaa gggctgaggg agttttgtat gaggaatcag acaaattacc aaccagaaca
     8161 ttgcataaag ttcgacttat gcctggaaga ggcccatggt atgagataga tactaacaaa
     8221 cataatgtaa tctcaatgag aatacttcca aagagaccta aggttttaat cacagagctt
     8281 ttgagagttc ttggatggga aacagatgat gaaatcagaa agctttttgc agatgtagat
     8341 acacataacg aatacaagta tatagaggct acccttgcaa gagattttac aaagaataaa
     8401 gaagaggcca ttataagtat atacaacaaa ttaagaccag acgaatcagt aacgctagag
     8461 agtgcagaga aatatataaa aagtaatttc tttaatgtta gaaagtttga tttaggtaag
     8521 attggcagat accaattaaa cagaaaatta ggtactacat gtgatatcaa caaaccagaa
     8581 gaggttatgc tctatccaaa ggatcttgta ttaatagtaa agagattaat ccagattaac
     8641 aatggtgtag tcccagctga cgatatagat cacctgtgta atagaagaat cagaagtgtt
     8701 ggagaaattc ttgagaggca gttaaatgca ggtgtcagaa gattagaaaa gaatattaaa
     8761 gataaaatga gtatgtacgg acaggatgct aaggttacac cttcaatgct tgttgggaca
     8821 aaaccaattg cagcatccat tcagtctttc tttggtgcaa atgctctctc aacattcatg
     8881 gaacagacaa atattctctc tgagcttgag aacaaaagaa aagtaactgc aggtggacct
     8941 ggtggtatct ctaaagacag agcaacattc tcaatcagag aggtacataa ttcccagtat
     9001 tctaaatttg acccagtaac aagtccagag agtgcaagta ttggtgttgt aacacagttg
     9061 gctatgttag caaagataaa tgaatttggg tttctttctg cgccttacag aaaagttcta
     9121 cacgaggtac agaatagcaa aacaaaatta cttaacaagg tagcttcaga agatattaaa
     9181 tctgggaaga aagttcttgt taaggcaggg acacttctaa cagaagaatt agtaaagaaa
     9241 gttgttgagc aaaaagatat taaaaatgta aaagtaaaac catttattga tgaagaggta
     9301 gtttactttg atgcaatgga agaagaaggc cattttatct caacagcaac tattaacacc
     9361 gacgaatttg gaaatatcat agatacaata gtgcctgtca gacataaagg tgacttcatg
     9421 cttgaggatg taaatacagt agattatgta gatcttcttg caagtcagca agcaggttta
     9481 ggtatggctt tagttccttt tgtttctcac aatgacgcaa tgagagcctt agtaggagca
     9541 aatcaacaaa gacaagctgt tcctcttgta aaacaagaat ctccacttat cggtacagga
     9601 ttggaggagg ttgttgcgaa gcagtctcta tggggaatat acgcagaaga tgatggcgaa
     9661 gtttactatg tagatgcaaa gaaaatgtct gttaaatata agaaagcagg agtcaaagat
     9721 tatgaccttg tctccttcta taggagtaat gacaacagat gtttctctca gaaaccagta
     9781 ttaaatgtag gtgacaaatt caagaaagat gatatccttg ttgatggtcc aaccatggtt
     9841 aacggagaac ttgctttggg tatcaacctt aaagcagcac tcatgttcta cgagggatac
     9901 aactacgaag attcagttat catttctgaa agaattgtta gagatgacct tttaacatct
     9961 gtacacatta gagaatatac agttgaaatt agagatacag aattaggacc tgaggtaatc
    10021 acggcagata ttccacatgt taatgacaga atacttcaaa aacttgacgc aatgggaatt
    10081 gtaagaaacg gtgtaagggt caagtcggga gatatcttgg taggtgtagt agctccaaga
    10141 ggagaaaaag agctaacagc agaagagaga ctcttaaggg caatcttcgg agagtctgca
    10201 agcgatgtta gagataactc tttgagaatt cctcatggag aagatggaat tgtaattaga
    10261 agtcagattc tctctgcaga gaagggagat aaactttctc caggagtctt gcaggaggtg
    10321 aaagtctggg tagcaaatac aaaaaagatt aactttggag acaaaatcgc aggaagacac
    10381 ggagacaaaa ataccgttgc tgcaattaga cctatcgaag atatgccttt cacagaagat
    10441 ggagaacctg tagacttagt tcttactccg acattcctaa aaagaatgaa catgggacag
    10501 gctcaagaaa ttcattatgg aagatatgct aaagaattgg gtgagaaatt agcattccct
    10561 atatttgaag gtaaaaacga tgaatggttg aaggaagagt taaaaaaaca tggtttagag
    10621 atgaatgaaa agattacttt gtatgatgga agaacaggaa agaaatttcc aagacaaatt
    10681 gcagtaggaa ttaaatatgt actaaaacta catcatattg cagacgaaaa ggtgcatgct
    10741 agatctacag gaccatacac tgcagtgtct cagcaaccac ttggtggaaa agcacaaatg
    10801 ggtggacaga gattcgggga aatggaagtc tgggcactag aggcacacgg agcaccttat
    10861 gcacttcagg aaatgttaac cattaaatct gatgatgtta agggaagatc cgcagcttac
    10921 aaagcaatta ttcatggaga gaagattgag aatgtaaata ttccagaatc attcaaagta
    10981 ctctctaagg aatttaatgc actatgtttg aatatggatt taatttcaag taaatcttcg
    11041 acagaagaag aggaggtagc acaaaatgaa gaaaatattt aacgagaaga aaaatctaaa
    11101 cgaagtagat atattgaagc ttacattagc ttctccagaa cagattctta actggtctta
    11161 cggagaggtt acaaaagcag aaacaattaa ctatcgtaca tttagagcag aaccagatgg
    11221 tcttttctgt gagaagatat ttggaccaac caagaattat gaatgctact gtggaaaata
    11281 caaaaagatt agatataagg ggattgtttg tgataagtgt ggagtagaag ttacaaccca
    11341 agatgttaga agagagagaa tgggtcacat tgaattatgt gttccagttg ctcatgtctg
    11401 gtttgcatac ggtattccta acaagatgtc tatcgtttta aacatctctc ataaaaaact
    11461 tctttctgtt atctactaca ccagatatat ggtagtaagt gtagagaaga atagaaaagc
    11521 agggataatt gagaaagcca atgcacaaaa ggaagaagaa gttacttcat tgaatagcca
    11581 gctagaagaa gagctaaaaa tgactagtga gatgtatgat gaccaaataa aagaagttaa
    11641 aaagaacaaa ggaaagaata ctgaattcaa aataagtcag attgagcata gtagaaaaca
    11701 aggattagca aaaattagaa gagaattcgc agaaaaagag gaagaattag agtcagcata
    11761 tacaaagatt cttcttgtta ttgagaaatt aaaagttgga acagtactct cagaggatga
    11821 gtatattgag ttagaggaga gagatctact cttctttgaa gctatgatgg gagctgaggc
    11881 aatcaagaaa ttacttgaaa gcttggattt agagaaagag attggaaaat tgaaaaagag
    11941 agcggaaaaa gagaaaggtg agagaaagct agctacaata agaagaatac aatacctaga
    12001 agggtttaaa aagaataatc taaatccagg gtggatgata ataactactc ttccagtttt
    12061 acctccagag ctaagaccaa ttattccttt atcaggtggt aaatttgcga cttcagatct
    12121 taacgatctt tacagaagaa ttattaacag aaacaatcgt ttaagaagat taattgaaat
    12181 tggagcacca gatgtaatct tgagaaatga aaagagaatg ttacaagagt ctgtagatgc
    12241 tttgatagac aactcacata gaccatcaaa ggctatgatg aacaacaaga gattaccata
    12301 tcagtcacta acagatgatt tgagaggaaa gaaaggtatc tttagaaaga atcttcttgg
    12361 aaaacgtgta gactattctg gtcgtgcagt tatagatggg gatccaagtt tgaaatttga
    12421 tcaatgtgga cttccaaaat ctgtagcatt agaaatgttc aaaccatttg taatacagca
    12481 acttcttatt agagaaattg ctccaaatgt gagaatggct aaagaaatga ttgaggaaga
    12541 ggaagattta atctgggata tattggaaga ggtaattaaa aacaaaccag tacttctaaa
    12601 tcgtgcacct acattacata aatatagtgt acagggtttc tatccaaaac ttgtagaagg
    12661 agaagctatt agaatacacc caatggtgtg taaagccttc aacgcagact tcgatggaga
    12721 ccagatggct gtacatattc ttctaactga cgaggctaga aaggaagcag agagagaaat
    12781 gatgtcttct aagagtattg tgaatatctc taacggtcag gtgcttgcaa gtcctgcaaa
    12841 ggacatgttg cttggattct acctaatgac agatttcgaa ccaaagaaaa atccaagaat
    12901 atatgaatca gtagagagtg cagtaaaagc atacgagaga gatttgattg atattaaaga
    12961 agagataata gtgagagcta agggtgagag attaaataca tcagttggta gagtaatatt
    13021 taacagcatt cttccagaag attacaaatt cttaaatgac agagtaaatg ttaaaacagt
    13081 tatgagaata attgacgaca tcaagaactc aagacctatg gaagatgtag tagagctttt
    13141 ggataatatg aagcttctag gatttaaata tgcaacagat ctaggattct ccttctcaat
    13201 gggtgactgt aatgtcgatt tcgatatggt ctccgagctt aagaaaattg aagagaaaga
    13261 cgagcaactg caggagaact acttacaagg attgatgaca aagaatgaga aaataaatat
    13321 ctctacaaat atgtgggatg acttctcaaa tgaattagca gaaaaagcat ggcagaaatt
    13381 ggagaaaaac aatcctgtat tcgaaatggt tgaatcaggt gctaatggtg gaaagattca
    13441 ggcaagacag gtattgacaa tcaaaggtgt agttagagat tctagaggaa acttggtacc
    13501 aatgccaatt aagagcaatt atagagatgg attaagtgca tttgagtact ttgtagctgc
    13561 aaatggtgga agaaaaggta ttgcagatag atcccttaag acatcatctt ctggatatat
    13621 gacaagaaaa cttgtagatg tagctcatga tgtaataatc agaaaggacg attgtggtta
    13681 cgaaggagaa ggacttgttc taaagagaga cgatgacaga agaatggatt tcgtagatag
    13741 aatcttcgga agagttcctg caagagatat taaaataggt aaaaaggttg ttgctaagaa
    13801 aaatgaagca attacaaaag agattgcttt ggaaatagat aaatcagatt taagtgaaat
    13861 ttctgtgaga tctccaatgt tatgtgattc accaattgga atttgtaaga aatgttatgg
    13921 attaaacctg gaaaatggac aagagattga aatgggtaag gcagtaggcg taatagcagc
    13981 acaatcaatt ggagaacctg gaacgcagat gactatgcag acttttcata agggaggtgt
    14041 tgctaaggtt gatatcacac agggtcttcc aaggattgaa gaattattcg aggctagaac
    14101 accaaaagca gaagcagaca ttgcctctat tgatggaaaa gttcatgtag agatagccga
    14161 agatgaatca gctacaatct caatctcagg ggagaagaaa agtacaagat tctatgttgt
    14221 atctaaagcc aagaaagttc tggtagagaa tgagcaggaa gttaaagcgg gtcaaataat
    14281 tttcatagat gctgaagaga atgaaaaaca agctcctttc gatggaaaag taaccctaac
    14341 tggtggaatt atgggaataa atggaacaat caaggcagag gagatagtat ctgtactgcc
    14401 aggtgtatcc atacttgtag aagatggaga aacaataaaa gctggacaac aattagtaga
    14461 aggttcagta gatcctaaga aactctctat agtagcagat ataattacag cacaaaaata
    14521 tattcttgac gaagttcaga aggtatttaa tgagcagggt gttccaatcg atgatgtaca
    14581 tctagaaatt atcctcagac aaatggcaag acttggaaaa gtagaagaat caggagatac
    14641 agattctcta gtaggatctt tagtaaatag attcttagca gatgcgaaga atagactatt
    14701 actaagccaa ggaaagaaca ttgcattggt aactccaaag ctatttggta tcaagacatc
    14761 atctctaaat acagaaggat tcttgtcagc catgtcattc caggaacaag taagagtact
    14821 aacaagtagt gctatattag gaaaaacaga tttccttcgt ggtatgaaag agaatgtaat
    14881 catcggaaga ttaatacctg taggagagag agctgagatc aaagatataa atgatgtagc
    14941 agagcttaaa ttttgattga ttttgttata taataccgac catgccaact attaaccaat
    15001 taataagaaa acctagaaaa tctaaagcaa agagagttaa gcatatagca cttgctacga
    15061 actttaatcc tcttaaaaat agatattcaa gagagaagaa cccttttaag agaggtgtat
    15121 gtcttcaggt aaaaaccatg acaccaaaaa aacctaactc tgctttaagg aaagtagcaa
    15181 gagtaagact ttctaatggt atggaaatta cagcatacat cccaggtgag gggcataacc
    15241 ttcaggagca ctctgttgtt cttctaaaag caggaagaaa aagagatctc ccaggtgtta
    15301 aatatattgt tgtaagaggt aaatatgaca ctgcaggtgt acaaggaaga aagaaagcaa
    15361 gatcaaaata cggtgctaaa tcacctaaaa atgcagacta gttttaatta aagaatataa
    15421 aaatgagagg aaaaagagca aaaagaagag aacaagtagg agacagaaag tatggtaatc
    15481 taactatctc tagattgata aataaagtaa tgcaagatgg taaaaaaaca attgcggagg
    15541 atttagtata tacagcatta aacagtgctg ctgaaagtgc aaaggaaaaa gatatagcaa
    15601 tatttatgaa tagagtaata gataatgtta aaccagcact tgaaattaga gcaagaagag
    15661 taggaggagc taactaccaa gttccagtac ctgtatctcc aagaagacaa gagacacttg
    15721 ctattagatg gatagttgat acatcaagag ctaaatcagg cacatcattc gataagacat
    15781 tggctacaga gcttctaaac gcatacaatg gtgaaggtga ggctattaag aaaaagcagg
    15841 atactgaaag aatggcagaa gcaaacaaag catttgcaca tttcaaatgg taagcttaag
    15901 catagaattt atattgattt aagaggggct caggcccctt tttcatttag atttaacata
    15961 ctaggggtta cttagtactc tctatcctct cccaaccagt agaaggcata tcttttcgta
    16021 atatatctct ctcccaaaat atctttatcc aagttattaa cctaacagga aagagaagaa
    16081 gagaccatac gaactcttta acatttcttg gatatttcag aacaaagaaa gcatatggta
    16141 actctatgaa aaagcttcta ctctgcttat ctttcttgaa tattccaaac ttttttaact
    16201 gcttaaaacc tccaagagat ctaactttct gcttgtagta atctttcaaa ttagtaggat
    16261 atttgacata acaggtagca ttcggattat atgcaatttc atacccatca ttccgaagag
    16321 agtatgagat atatgcatca tctgaaagta cattatctgg aatttttaga tcaatatttc
    16381 ttatagccat gatatatcca ctcatgggat agaagctttt accactcaca taataaccgt
    16441 ttcttcttac ttcttgcata gtatttcttc tcctgtggtc tcctgactct gaaagaagat
    16501 gtccccaata tccaaaaata ttttctctac tatcctgaga cactggtctt ccacttactc
    16561 ctcctatctt ggcattctca aatggtttaa ttaattctgc tactgcattt tctccaaaaa
    16621 aagtatctcc atctgtaagg ataataatat ctccctttgc tttcttgagt gctaatttaa
    16681 gagcatacgg tttgcctttg tgaggatcaa tgatatggtg gtactcacta ttggaaagtt
    16741 taagtatgtt agccattttc ttaccagctt tgagagttag ttcatctggg gatacttgta
    16801 ttatctcgaa aggtttaata atgccagaat attttttatc tgctatacac tgaatacatt
    16861 ttccaatggt atgcggttct ttaaaagagg taataattac tgaaatcata cgatattata
    16921 cctgttatgg aaaaatattt taagaatact acttaggttc ctctgttgga gtctctgctc
    16981 cctcctctgt aacttctgta ccttctgcaa cctcttctcc ctctgcagca acaggagcaa
    17041 taacttcttc ctctttctga ggttctgtga ctgtaacgat tgtagatttc ttagtatctt
    17101 catttattaa gctcattccc tctggtagtt taatatcttc aacagcgata ctctgtcctg
    17161 ggtgatctaa accagaaata tctatttcaa taaatggctt aagatcagat aatttacatc
    17221 taacatcaat actatctaaa gcctcaacca aaacacctag attattcttt actgctggag
    17281 aaataccaac aatatggaat ggaattgtaa ataccatggg tttggactca tcaatctcaa
    17341 agaaagaaat atgtcttaat tcttctgtca taggattaaa atctatctct ttaactaata
    17401 cttttgatgc tttaccatct aattctgcat cataaatagt tgtggaagtt gcttctctag
    17461 atatctgtcc cgctattctt gaatctatct gaatatttgt ggattcaccc tttgcattgt
    17521 atataactgc aggggtaaga ttctctttca aaagattctt acttttctta ccaaggatat
    17581 ttctttttgt gaatacaatc ttttccataa tggataggag tatatcaaag aatgggggat
    17641 ttgacaataa ttcagcaaat atttgacact caaacttttc cacgatatac tttagcacct
    17701 aaacttattg tctgctaaaa gtgaatataa cagaaaggca aaatgccatt ttaatggcag
    17761 taattaaaga gttcatggat gatgcaaacg aagtaggttc gctatccttg gttgagaaat
    17821 acaaccttgg agtaagctct gcgactatta gaaacgaaat ggtcaaatta atgtctctag
    17881 gacttttgga aaagaatcac atatcatctg gaagattacc aacagaccaa gcattgaggc
    17941 tgtatgtgtc taaaataata gataggtcat ctctgaaccc tcttgtttct gtagaaataa
    18001 gacaggggat ctttagggac agattctcta aagatattgt tattaaatca attctacaaa
    18061 tacttgccaa ggaaacagat tctgtagtat tcctaatact agatgatgat gttagatact
    18121 atggattagc caacattctt gcatatgagg aattccaatc aatcgagaaa ataaaaagtg
    18181 taattagtat tcttgaagac gatgtatttc ttaaaaatat ggctcagaaa tattcaagtt
    18241 ctggagtgtc acttctaatt ggtgaagagt caggtgtagg tacactgcaa gactgtgcta
    18301 tggcttttac taagataccc ttctggaata aaagcaattc gtatgtaggt gttattggtt
    18361 caaaaagaat ggattactct aaagtaattc ctgctttaag agaggtaaaa aattctcttg
    18421 aaaatgcaat gtctggatgg agataaatta aatacaagaa atatgaagaa agaactagaa
    18481 agcaaaaaaa taaaagattt agcactaata atagataaac tcgaagatga aaatttagta
    18541 attaaggagc agatgcttcg agcactagct gattatcaaa atcttgtaaa aagttcagag
    18601 aagagaaatg aaatcagata ttttcaatta aagaaatctc tgagtgaatc tgtaatacca
    18661 tctcttgatg ctcttaactt agcaataaag gcaagtaaag aattaaaact agatgaaaag
    18721 acaaaggctt ggttagatgg aatattggca acgatacagt cattagaaaa atcattctcg
    18781 gagataggaa taaaacagta tttaccaatc aaaggggata aatttgatcc aacaaaacat
    18841 gaagcagtag caaccgtaga aggtggaaaa agtggtgaaa tatatgactt ggtacagcca
    18901 ggttatatat tagacaacac tgtaattaaa ccagctcgtg ttgttgtttc aaaataaata
    18961 taaattttta ttaataaata tatgtcaaaa atcataggaa tagatctcgg aactacaaac
    19021 tccgtaatgg cctacatgga aggtggaaag ccaaatgtaa ttccaaatgc gcagggtgga
    19081 agattaaccc catctgtagt tgcaattggt gacaaaggag aaatccttgt aggaaatcct
    19141 gcaaaaaacc aagcagtaac aaatccagag ggaacaattt actctgtaaa aagattaatc
    19201 ggaagatcat gggatgatcc agaggttaaa aaagataaag aacttcttcc atttgaaatg
    19261 aggaaatctt cctcaggtgg tgtagaggta aaaatgaaag acaagtggta ctcaccacag
    19321 gagatttctg caaagattct cgcaaaaatg aaaaaagatg cagaagattt cctaggagaa
    19381 gaggttaaag aagcagtaat aactgtacca gcttattttg atgactctca aagacaggct
    19441 acaaaagatg caggaaagat tgctggatta gatgtaaaaa gaatcgtaaa tgaaccaaca
    19501 gcagcagcgc tagcatatgg ggtagaaaaa ggaaaagaag aaaaagttgc aatatttgat
    19561 cttggaggtg gtacctttga tatctccata ctagagattg gcgatggagt atttgaggtt
    19621 ctctctacaa atggagatac acatcttgga ggagatgact ttgaccagaa aatcatagac
    19681 ttcttagctg accaatttaa ggcaaaagag ggcatagatt taagaaatga caggacatca
    19741 cttcagagat taagagaatt tgcagaaaaa gcaaaagtag agttatcaac atcagaagaa
    19801 acagagataa atatccctta cataacagca gatgcaaatg gaccaaaaca tttaaaagaa
    19861 aaattaacaa gatctgaatt agagaaacta acaagcgatt taattgaaag aactgttgag
    19921 ccatgtaaga aagctcttaa agatgcagga ctttcaatca aggatgtaga tgaaatactt
    19981 ctagtaggtg gtatgacaag aatgccagca gttattaaaa aagttgaaga actttttgga
    20041 aaagaagcaa acaagggagt aaacccagat gaggttgtag ctgttggagc tgcaattcaa
    20101 ggaggggtat taggtggaga tgtaaaagac attactcttc tagatgttac accactgtct
    20161 ttaggattag agactctagg tggaattatg acaaaattaa tagagaagaa cacaacaatc
    20221 cctgtagaaa agaaacagat attctctact gcagcagata atcaaccagg ggttgaaata
    20281 catatccttc aaggagaaag agaaatggca gcagataaca agaccttagg tcgatttatc
    20341 cttgacggta tcccacctgc accaagagga gttcctcaga ttgaggtatc tttctctata
    20401 gatgcaaacg gtatcctaaa tgtaaaagca gtagatcttg caactaagaa agaacagaaa
    20461 attaccatca cagcatctac aggattaaaa gaagaagaga tagagaagat ggtcgaagat
    20521 gccaagaaga atgctgagga agacaaaaag aagaaagaaa gagcagaaac aaggaacgag
    20581 gccgatactc tgtgctttgg tgttgagaag ctaatcaaag agaatggaga caaaatagat
    20641 tcttctgaga aggaagatct tgagaagcag tccgaagagc taaaaaagat aattgagaaa
    20701 gaagattttg atgtggaaga tgtaaagaaa aagacagatg aacttatgga gaaaatgcag
    20761 gaagtaggaa agaagatgta tgaaaaggtc gctcaagagt caaagcctga ggaagaaaga
    20821 aagaaagaag aaaagaagga cgacgatgtc aaagagggag aggttgtaga ttaaccttct
    20881 ggcgtgtaaa agaattagag gagactgttg gtctcctctt ttttttatgg tacgataaat
    20941 agaataatat ttactacact gccgagatat gcttagagat tcagagggca ccaagaaaga
    21001 aaaaattgca ctcccagtta ttcaaaaata cctatttctt gccatgctat tccttcttcc
    21061 tctctccata attccattcc catgggattg gactgaaaag ggtatgtctt tggttattct
    21121 catattttca acgattatta taggaattga aataattaaa ttgatatggg atgggaaagc
    21181 cacacttata aagagccttt tagatatggg ctcgtttgcc attttaatct ctcttatact
    21241 gtccacctta ctctcgaaag atattaattc cagcctttgg ggagtagaca acagactggg
    21301 ttcaggatta atcgttttca tagcagtaat cttagtatct tttagtgcac gaacattcat
    21361 acataatatg cgagatatta agatggtaat actctccttc ctaggaggtt tgtttatttc
    21421 aaatatcttg agtatattct cttttctagg atcaaacata tgggaaataa ttcctgtata
    21481 caaagaaatg catcaggcag gtttaccact cttgagatct gagaaagttc aagtcctgat
    21541 taatatggta gggatattac tttctttggg gattattggg gagagaatgt taaggcaaga
    21601 aggaaaaagt atagctatga cactgtgcta tctctttctt gttccagcta ttgttaatct
    21661 ttgggtattc tcaattcatc agggattagc aatacttgta ctttttacaa ttattctaat
    21721 tttaatctta attttagtac taccaagatt aaagataggg cgaggattaa aaagagatct
    21781 tgtagttctt tttattggta tatttctaac cattgcactg ccagttatat tacttcaaat
    21841 accatctttg agagcaatca ttattcctag gggtgtaaat ctagtagcag aggtatctct
    21901 tggtgcggat atctcttgga taattgcagc ttctgttttt gttgattcgt tatggaaagg
    21961 tttatttggt cttggagtag atacatatac aattgcctac aatatgttta aaccactaaa
    22021 tgaagcactt atctctttta acagtgtaaa tttttacttt gcaggaaatg aactctttac
    22081 taaattctca aatggaggat taatatggct aatcgcttgg ggattctttg gttttctttt
    22141 ggcaaaactt ctaaaagatg atttaaggag atttagatta tatcaggagg acaatgctgc
    22201 attccttctt gttatgtttg actttactct tgtatttctt tttctttcct ctctgtttgt
    22261 tacatattct atatatgtaa tatttgttct ttttgtatta atttctctac atagtatttt
    22321 gaaagatatc cttggtaagg gcagtggaga taaatttgta tttaaacttt gggcagttaa
    22381 tgtttcttca aaagtagaat ctggaaaagc gatgtacaat atcaatgttt ttcttacagt
    22441 agtaacggtt ttaataactt gtggattctt ggttctttgg ggatcaaaag taatatcgag
    22501 cgcatattta ctgaaagctg aatcatacta tatcgaacag aagaagatat atgccgagaa
    22561 agaaccttca cttaaccaga gggaagactt tgtaaaacga atgactggtt actactctaa
    22621 agcattgaag tttgacaaca aggatcctct tgtgaacaga aaattgggat tgatgtatct
    22681 tgaacaggta ggaattgcag cagagaaata ttcaaaaaat aaggagtctg aagaagcaaa
    22741 tacaataata aaaaatattg gacagtggaa gaactatgct atagattaca tccgaaaaag
    22801 tattgataga agtccagcta tatatgcaaa ctgggaggct agagctaggg tatatatggg
    22861 actagtaggc tttggcttct atgattacac tgcagatgct cttttctctc ttgaaaaagc
    22921 aatagaatta aatcctttga attttgaatt gtactactac aaagcacaac taaatgtaat
    22981 aagtggagat aaagatggag cactgtctgc tttgactcaa gttttaggta ttaatccaca
    23041 gcatgttgca tcaataatgt tagcaggaga gattaataaa gagaaaggta atattgaagt
    23101 atatgaatca tacttaaaag cggcaaagaa gatattggag atacaaggta acacagatat
    23161 agaacaatac aaggaaattt cgaatcaatt gaatgaatta gcaaaagatg attcgaaagg
    23221 ggaagtgaaa gaagaagaaa ctgcagaaga gccaactgag gaaactacac cagtcgtgga
    23281 gacagtacct gctgaggctg gagagtaata tggtttacct ctaaaaagac agagtgcatt
    23341 tactgaaaca tctgcttcct tctttattac ctttgagata ttctctagag tacttcctgt
    23401 cgtgatgaca tcatctaaaa ttgttacaga attgtactta tctagcaatg acctatcttt
    23461 tatatagaaa ttagacattg tttcttccaa cctactgtct ctatctttca aagattgatg
    23521 ccctttctct atttttcttc caattaaatc aaaagagaaa tgacatttgt aatgtttaga
    23581 aatattctca acaagtagct ctgtttgatt aaatcctcgc tcacttcttc ttaagaagga
    23641 tattggaaca ggtatgaaaa gagagttatt aaaatcaata cccctgatac tttccaacaa
    23701 aaattcagaa atatatctac caatatcttg catccctttg tacttgtaac tttttagaat
    23761 ttttgaagat atcttgttgt attcccacgc tacaacaact ctatccaagg agtaatcttt
    23821 tttacattgt gtatgtgtac agtaatttgg agatattctt cgacagatat aacattcagg
    23881 cagatccttc ctgaaactct tcttacatgt attacaaaga tattcaccat atttgccaca
    23941 aatatggcaa gattttggat atactaaatc tagaagagac gacatcataa ctcaatttat
    24001 caataaaaaa ttaaaaacaa attaattgat aaaaaattct ctttaaatta tgatattatt
    24061 cgcacagttg aacttaacct tgatatatac taaatgataa agcttggact taagaagaac
    24121 agcatttttt ttgtaatgtt tcttctcttc ataacaaaga tactaggatt tttaaaactc
    24181 agaaccattg ctcagctctt tggtgtctct catgaactag atatattctg ggcagctttt
    24241 actatcccag acatgatatt tactgttctt gttgcaggct ctattaatgc agctatcatt
    24301 ccaatatttt ctgaaatttt ctacaaaaag ggtaaagaaa gtttagatcg tttcttcaac
    24361 tatcttttca tagttctaac cgtgtcctgc cttctgattg caggtctttt ttttgtattt
    24421 acaccttgga ttacaaactc cgttattaac tcagaggtac tccagaaatt cttagatctg
    24481 tcaggcagaa taactcctgc agattaccat atgtttgtta cactcactag attgatgttg
    24541 ctttcaccaa tactgctagc aaccagcagt cttgtaacag catacctgca gattaagaaa
    24601 gaattcttaa caacctctct tgcacctatc ttctacaatc taggaatgat aattggtcct
    24661 atcatttttg ttgtatttgg aaatcttgga gtagaaggaa tcgcaatatc tgcggttatt
    24721 agctctgtat tacatctcct agtacagctt ccccaatttg caaagcattt taatgatgga
    24781 ttttcactta gtcctagcta ttttaaaaat gcgttccaag atatacatat atggagaaca
    24841 ttcaagttag caattccaag aacaattggt atattaggag aacagataaa tggattagta
    24901 aataccctaa ttagtttctc tcttacagct ggtgctctta gtgcatacag atttgcaatt
    24961 agtttgcatc tttttccaat aaatataatt ggaagtgctg tagcacaagt tgcgctacct
    25021 gatttggcac aacatagtgg agatagaaag aagtttgtta agatcttgga ttcttccata
    25081 cagtttgctt tgtatattgt atttccaatt gtggctatga tgatgatact taggctccca
    25141 atagttcgtt tagtatatgg ctctggtgcc tttgactgga gggcaacaat tttaactgca
    25201 tggtgtcttg tcctcttagg attttccata attggacaaa ctgtagcaca gatagttctt
    25261 agggcgttct atgctcttaa ggagacatgg cttccccttg tagcaattgt tatcagtatc
    25321 atagtaaaca tcgttcttgc ctactatctt accaatttct ttagccatta ctatgactgg
    25381 agaccaatat ttgatcagat ggtataccaa atttcttctg caaacggaga gggcttcttg
    25441 gtagtgctga aatcctttgt aaaagatttt cttagatggt catcagacag aggaacctct
    25501 gacatggcag ttggtggttt agcattaggt tttgggatct cgtattttgt agagatgata
    25561 cttctcatgt tattgcttaa ttttaaggga aagataatta catggaagag gacaattaaa
    25621 cctatcttag taaagattct aaatacagta ataatggcaa ttggaatgta ttttgtcttt
    25681 aagctttttg attttcagct agacacgaca cgaacatggt caattattgt tttatcagca
    25741 gcagtatgct catacggtct actctcttac tggataggat ctaaaatatt tagaataaaa
    25801 gaggtagata aatttgaaag caggatcgtg gtaatattct ctaaattttt cagtagaggg
    25861 gagaaagatg cagagactgt ttaagatatt tggaatcttc cttttactgc taatttttct
    25921 tccaatcttc ttaacaggaa tgtatcttct ttgggaaaga gagttcagtt taaacctttc
    25981 gagcaaaagt tgtgtaagta ataaccaggt taaagagatt tcttttgatg agaggataag
    26041 aaattttgtt ttttcctctg atagaagtga ctacatacaa tttacgaagg aagaagtcct
    26101 ctatttaatt tcctccgaat ttggaaaaga agagggtgat atagttgtaa aagatatgtg
    26161 tgtagattct tcaaaaggag tatggaaagt gtatctgaat ttaactgttg ccgggataaa
    26221 gaatgtctgg tttatctttg acataataaa agatgataga gaaacagcag aactgtatgt
    26281 agacaacctg tatcttggaa attttcagtt accttccttc atctcttcct cacttaagga
    26341 gaaaattaac aaaggaatta gtgatgcaat tgttcttgta aatgaaaaca gtttctctgg
    26401 aaggagtatt acaaatattg aacttttaga agaagatata gtggtaaaag gtgtgtatta
    26461 ggcttgtcca acaacataac ttgtgataca attctctagc aatccttaaa ttaatctgct
    26521 aataaatatg tcaaaaaaga tcaacataat gcctctagga aaaagaatct tagcaaaacc
    26581 agatgcaatt gaagaaaaaa cagcaggagg tttagtatta cctccaaatg taaatgatga
    26641 acaacgatct gctactggta ctgttgtagc tttaggaaaa gcaaaatcat ttgatgtagc
    26701 aatcggagac agaatatatt tcaaaaaata ttcaccagag gaaattgaaa ttgaaggtgt
    26761 caaatatctc atactagata ctgacgatgt actcgcagtc ataaaataaa tttaatacat
    26821 acacaaaatg gcaaaagcaa tcatatataa cgaagaggct agaaaagccc taaaagaagg
    26881 tgtggacaca atatcaaatg ttgttaaaac aactcttgga ccaagaggaa gaaatattgc
    26941 catagcaaaa agctatggat cccaaatcat aaccaaagat ggtgttactg tagcaaaaga
    27001 aatagaagac agagatcctt ttaaaaatgt cggagtcgaa atgataaaag aagcatcaag
    27061 taaagtaaac gatcttgcag gagatggtac aactacagta gcagttcttg ctcaagcaat
    27121 cgcgaaccta ggctttagaa atgtctctgc aggatctaac ccaattgctg tcaaaagagg
    27181 tatagataaa ggtgtggaga tgatagttaa agaacttgaa aagaaggcta agaagatttc
    27241 tggtcttgaa gaaatttctc aagtggctac tatatctgca aacaatgata aagaactagg
    27301 agacatgatt ggtggagttt ttgagaaagt tggaaaagat ggagtaataa cagtagagga
    27361 agcgaagggt tttgatgatg aggtagagta tacagaaggt atggaatttg ataacggtta
    27421 tgttagtgca tactttgtaa acaacgcaga gactctagaa acagttatgg acaatccata
    27481 catcttcata acagataaga agctttccaa tcttcaagat atccaagcaa tagctgagaa
    27541 ggttcttgga gctgcagaca gaccattggt cataatcgca gaagatattg agaaccaggc
    27601 tcttgctaca ttggtagtaa acaaactaag aggaacatta gatgtagtag cagttaaagc
    27661 tcctggattt ggagatagaa agaaagaaat gctgaaagac atctgtgtat taacagatgc
    27721 aaaattcatc tcagaggagc ttggaaatac cttagacaaa gtagagatta cagatttagg
    27781 ttctgcaaag aaggtaattg taactaaaga gaagacagtt attgtagaag gaaaaggaag
    27841 taagaaatca ataaatgata gagttagtga aattaaagca caaatttcta aaacatcttc
    27901 agattatgat aaagagaaat tacaggaaag attagcaaag ttagtaggag gtgttgcagt
    27961 tataaaagtt ggtgctgcat ctgagacaga agcgaaggag aaaaagatga ggattgaaga
    28021 tgcaattaat gcaacaagag cagcattaga ggaaggtgtt gtagctggtg gtggtttagc
    28081 actacataat gctaaatctg ttcttaatga tgtaaagtta gaagatgtag atgagcaggt
    28141 tggtatagat atcctaaaga gtatccttag tgaaccgcta aaacagattg cggagaatgc
    28201 tggtgaggat ggtgcagtag tagctgctaa ttgctcagga aaattaggct ttaatgctaa
    28261 gacaggtgaa tatgtagata tgatagaggc aggtataact gaccctgtaa aggttacaag
    28321 gttggcatta gtaaatgcag catctgttgg tactatgcta ataactacag aggcagtggt
    28381 agcagaaatt ccagaagaga aaagtaaagc accaatgagt ccagatatgg ggatgggtgg
    28441 aatgatgtaa tctggtatgt aagtggaact ataaaaagga ggtataaaag cctccttttt
    28501 tttaataaac aatcttaacc caattctcct tcaaccatgt atctgtatcc ttctgtaatt
    28561 gttctaatcc tctttttttt aattccctat ctataatctt tcccttatgt ggattattaa
    28621 actgtttctc aagcggtgaa ctaaaaccat gtgcgtaatg acacatctca tgtgcaatag
    28681 tagctcttac aatatcttct ggaacaatct cattttggaa atatctcgtt acagttataa
    28741 ctgtaatact cttctcatcc tgtgttaaaa aatcatcctt tctgttttta attaatcctt
    28801 ttatctttgt tcttccattt gctaatttaa tactccctaa ctgtcttttg gaatatttac
    28861 caaacttaat aacaaccata ttctttcttg gtatatcaca gaaatggttc tcccataatt
    28921 catacattaa attttctaaa aattctctat ttctcataca actgttctct ctcccatgtt
    28981 atcatattca aatgatacta cagaaataca agaaaaaaat ctctacatta aaaaaatctc
    29041 tttcatttgt atacggtcga tatacaacag cagcaatatc aagagatatt ctctttgcca
    29101 tatctacggc atctgaactt tacagtatta cccttctagg taaattcata gatgaaaccg
    29161 ctaaaatttt gctctcttgg gaaagtttta atttaacaga atatcttgga acaaaatctt
    29221 ttctgtacct agttctaata cttgccctct ggggtgtaac ccagatatgt gtacaagcga
    29281 gagaatattt ctacaatgta atatacgaaa gaacttggga agatgccaaa tacatgatga
    29341 tggctaaagt ctctagatca aatcttcagg atgtggagaa agaagaattt caagattatt
    29401 taacatatgc ccctacattt tctatagata gattgataac agcatacagc gatttctctg
    29461 taattctaag caatattatt agattaattg gagcattagt aataatctgg ggtgttgcgg
    29521 gatggtggtc cttaattttg gtaattctag tttttccaga agtaattgca gtacatttaa
    29581 ggagaagaga gattaaaaaa gaacaagata acgctgttgg taagtttaaa ttccttaact
    29641 acattcaagg tgttgctttg cagatttcca acttctgtga actaagggta aatgatacat
    29701 tctcttttct taaaagaaga tatacagagg agtatgatga tttcttggag ggatacctta
    29761 aaaaacaggc caatttctac caagacaaaa caatattttc cataatagac caagtattta
    29821 agtttgcctt tgttatctta cttttggcat tttccattgc tcagagatta acaattggta
    29881 ccttcaaagc actgtatgac tatgtagatg tggcatacaa cagtatttac agtattatga
    29941 atacagtatc tctacttgcc aataagcttg aatacattga tgaatttttt gacctaatag
    30001 aatatgaagg gtttggtgat catcaacacg ggaatgtgaa attaccgaaa ggtacgcctc
    30061 ttttagaatt cactaatcta gattttgcat atccagatga tccagatgta aaaattctca
    30121 aagatttaaa tcttgttgta aaaccaggcg aaaaagttgc attctttggc ggagacggct
    30181 caggaaaaag tacaacagtt aagatactct ctggtttgta tcaggtagtc tctggagatt
    30241 tccaaattgg tgggcactca gttagagaac tagatagagg ggaattaaag aagaagatag
    30301 cagttacatt ccaagatttc gtaaattaca atttcagtgt acttgaaaat atagttatat
    30361 ctgacaaaag aaagaagatt gatgaagagc tctacaaaaa ggtatacaca atttccaagg
    30421 taaaagattt tgttaaaaaa cagaagataa aagaatccca tttacttgga aaaacattcc
    30481 caggaggtat ggatctttca ccaggatatt ggcaaagact tgcgattgca aggatgcttt
    30541 acagaaacaa aaacatattc ataatggatg agccttttac tttcatagat agtatttccg
    30601 ctagtgaaat tctaaaggga atctttgaat tcttaggtaa agagagatct ttaatattta
    30661 taaccagaag tacagagaat ttaaaaatgt ttgacagaat atattacttt gaggatggaa
    30721 agatagttga agaagggaca tggagatctt tgatgaaaag aaaaggaaaa ctataccatg
    30781 aggttagttc tcttaattaa gaatatcctg taaaatatac cctagaatgg aaattttaga
    30841 tggtaaaaaa atatctgaag agattctgag taatatatct gatgaggtag aaagacttag
    30901 ggttcagaac aaaaggattc caagattgga tatgattatt gttggagatg actatggtag
    30961 tatcaagtat gttggaatga aagaaaagac agctagggaa gttggtatag aaggacaggt
    31021 acatcatcta ccattttctt caaatacgct agatattgtt aaccttatag attctctaaa
    31081 cagatttgat ggagtagatg ggttcatggt tcaattacca ctgcctgaac atatagatac
    31141 tcagttagta cttaatacaa ttgcaccaca gaaagatgta gatggtctaa cagcgattaa
    31201 tcttggaaaa ctttttcaaa aagatccttc agctatacct ccagcaacac cccttgggat
    31261 tatgaagctt cttgatgtgt acggtataaa gttgtttggg aaacatgtcg ttattcttgg
    31321 aacaagcaat attattggaa cgccacttgc cgctatgatg ttagaaaaga atgctacagt
    31381 atcgctatgt aatgcacata caataaatat acaggaaata tccagtaatg cggatatact
    31441 agtctctgct actggtaaag ctcttttaat taaaaaagat ttccttaaac caggctgcgt
    31501 agttatagat gttggatcga ataaacaccc agaaacaggc aagttagttg gagatgtaga
    31561 ttgggaagat gtaaagggta ttccatcata tattactccg gttcctggtg gtgtaggtcc
    31621 aatgacaatc tcctgcctca tgttaaattt aatgtcagct tacaaaaaga atgattcaag
    31681 acaagaaaat actagaactc attaacgatg agaagaagag acagagagag ggcattgagt
    31741 taatagcttc agaaaactat gtaagtgaat ctgtgcttga gtgtatgggt tcaattctta
    31801 caaacaaata ctccgaagga tattcaagaa aaagatatta caaaggtaac gaatatatag
    31861 atgatataga agaggaagca attagtagag ccaaagaact cttccatgct gagcatgtaa
    31921 atgtacaacc ttactcaggt tctcctgcaa atcttgccgt acattttgca ctactggatg
    31981 ttggagatag aactttaggt atggcattgg atgcaggagg tcatttaaca cacggattta
    32041 aggtttcaat aactgggaaa tattacaact ctgttagtta cggtgtagat gaacatggat
    32101 atatagatta tgaagaagta cgaagattgg caagggaaca tatgcctaaa ttaatatggg
    32161 cgggattctc tgcatactct cgtatcattg actggcaaag atttagagat atagcagatg
    32221 aggttggggc atatctagct gtagatatag cacacattgc aggattagtt gcaacaggaa
    32281 tatatccatc acctgttgga attgcagatg ttgtaaccac aaccactcat aaaaccctca
    32341 gaggcccaag gggagccatg attatgtgta aagaagagtt agctgagaaa atagataaag
    32401 ctgtatttcc aggcctacag ggaggtccac ataaccacac cacagcagcc atagcagtag
    32461 cacttaagga agcatctaca gaggaattca aggaatatac caaacagata gttaagaatt
    32521 ctaaagcact tgcagaagaa ctaattaaaa gaggttacaa aataatatca ggtggaacag
    32581 acaatcatct cttccttgta gatattgttg ctagtgtagg aatatctgga gaggaagcat
    32641 cttcattact tgagcagtca aatattacag taaataagaa tgcaattcca aacgatacta
    32701 gaaaaccatg ggatccttct ggtatacgta taggtacacc agcagtaaca acaagaggga
    32761 tgaaagaaaa ggagatggta aagattgctg agttcataga tgaagtttta agaaatagaa
    32821 aaaatgttag agcagaggtt aaggacttta caaaagattt tcatatacca ggtataaatt
    32881 aatactttta aaaatgaaat taacttacag aaaagattta aatattgcta ctattagatc
    32941 agaatcagat tggaacaagt tcaaagttca tacactatta cagaacccta cacagaaaca
    33001 accatcaatc aatgcatatc ttggagccag atatagtaga tctgctgact ccataacaga
    33061 tatagcatcg gagataatgg aatctggaac agatgcagca gaaaaattgg aaaaaatatt
    33121 ctctggttac ggtcataaat ctgtagggga tatggcagat ctttttctct gtatagagaa
    33181 tattcctatg tatacagcaa tgagaatata ttacatgtgt tctgttctct caggacaaga
    33241 aaggtctaca aggtatcaaa attttgaaaa gccagaattt attaaaattc ccaaagaagt
    33301 atgtgctaac tatgaagttc gtaaggagta tgaacgaata attctaaagc agatgcaaga
    33361 ttacagagag atgatgaaac caacaagaga agcactagaa aaagcattca agattaatga
    33421 agaaagtcct caggaagtgt ctgcacttaa ggcaagaagt tttgatgtat gcagatattt
    33481 tattcctctt ggaatacata ctagctcggc atatttaatg tctgcaagaa attggtcgga
    33541 attaattagc ttactatgtg caaatgacag tgttgtagaa aatgatgtgg cagatttaat
    33601 ccttaatctt cttggtgatt caaaattaga agtaaaggga tatttgaaag aagctgacaa
    33661 cctaataaga cacacagatg ccaattgttg tagaaagaat tcctcaaaag caatattaga
    33721 atatcttaaa gagagaatat ctagagagca agttaacgat atccctgaaa gtgaaattga
    33781 caagttagaa attgctcatg gtagtgatag cacagaagcc ttaatatcta actatgaatt
    33841 actccttaac ccccttggtt caaagcagga atttgaattt acagattttg atcaagaaag
    33901 tattggggaa ataatatttg acaaccatga ccatcacaat ctgttaggta atattggtca
    33961 atttggaaat attaagataa ctggatttgc aagtatagga acactgaaag atttaaatag
    34021 gcacagaagc ttagagagat ttattccatt tttccatgat gatatagatt tagatcagga
    34081 attaaatcga cctaatttta aatgcttttt cctttgcaat tatttagata ttaaatcaat
    34141 atcaaaactt aggggtgagt atgagaaaag attgacggaa acatataaga taatcaagga
    34201 atggaggaag aaggctaagg gaaatatgac aaaagaggtg tgtgatgagt ttacgaagta
    34261 tcttcttcca catgcacatt ctacaagata tgtgttctat ggttcgtttg atgacttgca
    34321 atatacgatt aatcttcgta caagaaatgg tgggcatatc gcatataggg ttttgacata
    34381 tgaatggtta aggcaattat ctttagcaga tccgatatgg aggcctttac ttaaaagaat
    34441 tgttgcaccg aagattgatg ataagcatca atttgtagat agaagttaaa ttaaaacaca
    34501 cgtatcatga tattgtcaga taaatcaatt aaagcaaaag taaagacagg agagatagtt
    34561 atcgaaccat tcgaagaaag atgtcttcag tccgcatcgt atgatcttca tttagacaag
    34621 gattttcttg ttttcaacag agagaatcat agcctaatag atgtaaaaga acctgttgat
    34681 gaattgatga agaaaatagt agtgtctgaa gaacagggta taattattca tccaggagat
    34741 tttatattag cgaatgttaa agaaattact ggtgtcgacg ataaacacgt aggaaggctt
    34801 gaggggaaga gctctcttgc aagaatggga ttaataatcc atacaacggc aggattttta
    34861 gatccaggga ataaattgcg tcttacattg gagatggtaa atctttctcc cttgccaata
    34921 aaattgtatc ctggaatgaa gatagggcag atagcgtttg aagaattaga tgaaaagtgt
    34981 gagagaccat atggaacaaa aggacttatg aataagtatg taggagatat gacagtaacg
    35041 gcaagtcaaa tgtggaaaaa ctttaaagaa gaagataatg aacaaaaaga ttaggaatca
    35101 atttccaata ttacaaagga agataaatgg taaaaagcta atatatttag atagtgctgc
    35161 tacatcccaa aagccacagt ttgtgataga tagtatctct gagtattaca aaaaatataa
    35221 tgcaaatata catagaagtt ctcatgtact tgcaaaggaa gctacagata tgtggaagga
    35281 agcacatcag atagttgcag attttttaaa tgcagaatcg tacaaggaga tagtgtttgt
    35341 aagaaatagt actgagggtt taaatctact agctaagaca tatggtaaag ataatctttc
    35401 aaaagaagat attgtaataa tttctgagat ggaacaccat agtaatatcg ttccttggtt
    35461 aatacttcaa aaagaaattg gttttgaaat taaatatatt ccagtaacgg atgattatga
    35521 tttagatatt aaatggttgg aggatactgt aaaggagttt ggttcaaaga ttaaaataat
    35581 atctgttgta catatttcca atgtattggg tacaaaaaac gatgtaaaga aaatttctga
    35641 aattggtcat actgttggtg cttttgtaat tgtagatgga gcacagagtg tcccacatat
    35701 tcctgtagat gttcaggata ttggctgtga tgctttcgtt ttttctggtc ataaagtata
    35761 tggtccaaca ggtagtggtg ttgtttactg taagcagaat attcttgaga agctttctcc
    35821 ttttatgggg ggtggggaga tgattctttc tgtaaagaaa gatggttttg aaatgaatga
    35881 actaccatgg agatttgaag ctggtactcc agatattgag agtggaattg tattagggaa
    35941 gacgatccaa tggtttaaga aaacgataga agaaattggt ggatgggatg ttttaataga
    36001 acatgagaag aatttgatga aggaattttt agatagtttt gatggtattg aatggtttag
    36061 attgtttggt aagaagagtg tagaggaaag gtatggagta atatctttca atattgatgg
    36121 atttggattt aaagggtgta aggaaatgga gaaggggaag aaaggaggaa gcgaaatagt
    36181 ggaatatatt agtgcaagag gcgttgctgt aagagaagga tatcattgtg cagagccttt
    36241 gcatgataga ttcaaagttg gtcctacaat gaggatgagt ttaggtatat acaatgataa
    36301 cgaggatgtc aaaacatttt ctaaattact aaaagaagct gtcctttctt cctactagat
    36361 agttggttgt actggaggaa cactctgtgg ctcctctgtt ggagtagttt cttcttttgg
    36421 tgctactgct ccgttattta ccatttgttg catgtctggt aagtttgaag gcaaaacagg
    36481 tgctgtttct tcaaccactg gtgctggagt ttcaactggc atagcattaa agccaggtgt
    36541 aggtgttgtt tcctcagcca ctggtgcagg agtttcaact ggtatagtat tgaaatttgg
    36601 tgtaggtgtt gtttcctcaa ctgctggtgt tggagtttca actggcatag tattgaggtt
    36661 aggagcaggt gttgcttgta cttgagtatt atcttttgcc attaaatctg ccaaatcatt
    36721 agactcagtt gcttccgcac tctggagttg tgtaggttgt tcatccaatt ttgtttgtat
    36781 tgtttcttcc ttaacctgtg gagtgtaaag attttcagag atattatcta tatctgtagg
    36841 acgactctca gctggagctg gagtttcctc tttcttctct tcaacctgag ttactacctc
    36901 ttctggtt
//
LOCUS       DAOQWP010000004        36752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_1320_length_36752_cov_8.041502, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000004 DAOQWP010000000
VERSION     DAOQWP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 36752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36752
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_36752_cov_8.041502"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKI16_00940"
     CDS             complement(<1..461)
                     /locus_tag="PKI16_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNR53039.1"
                     /translation="MSQIRLNENQKKAVEYNKGDLLIIAGAGTGKTAVITQRIINIIK
                     KDLAKPSEILALTFTEKASEEMKERVDVEMEYGYEEPWISTFHSFCDRVLREDGYHIG
                     LDSNYSLMSGAQSYIFLRRFLYDLPLDTLKPKGNPTKFLNDLIKHFSRLQD"
     gene            complement(606..1475)
                     /locus_tag="PKI16_00945"
     CDS             complement(606..1475)
                     /locus_tag="PKI16_00945"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNR53040.1"
                     /translation="MYLGIHVSSSGGIQNSIKNGEKYGVNSIQIMPTAPMRWATKLIS
                     DEEIEKFVNELENSNIKKILIHGIYLTNLARSDKQMFHLGKMGLVVYLDFAEKVAKEI
                     KKRKINSEILGVCFHPGSQKELSYEDSLERISYGMQWVLDEVPGTQKLLIESTAGTGS
                     NLGRTFTELKIMREGVKDKKRVGFVVDTQHTFAGGYDWINDINGVLSDMDREIGIENV
                     KAIHLNDSATALGSNKDRHADLGKGKIGEKTIKDILHNKKLANIPFILETPALKNDTD
                     TYKEVEVLKKLAN"
     gene            1515..1603
                     /locus_tag="PKI16_00950"
     tRNA            1515..1603
                     /locus_tag="PKI16_00950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1549..1551,aa:Ser,seq:gct)
     gene            1788..2003
                     /locus_tag="PKI16_00955"
     CDS             1788..2003
                     /locus_tag="PKI16_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HNR53041.1"
                     /translation="MPNLDNTGPLGQGPMTGRRLGFLPRFRGKRLGGSQVCKCPNCGY
                     TEPHTRGIPCTKKMCPKCGTPLRGEYC"
     gene            2005..2286
                     /locus_tag="PKI16_00960"
     CDS             2005..2286
                     /locus_tag="PKI16_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNR53042.1"
                     /translation="MRPRFRRRLGIQFKDTYFKPRGIPLRELSEVNVTDEELETLRLR
                     YIEHIDQEQAAKMMGISQSQYQRDLTSVMEKITQSLVYGYAISITKRES"
     gene            2333..2611
                     /locus_tag="PKI16_00965"
     CDS             2333..2611
                     /locus_tag="PKI16_00965"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNR53043.1"
                     /translation="MLKHIEQENIKELEKLLVPNRIQILHLLYKKDTCVCQMVKKLEL
                     KHNLISHHLKTLQEMGYISSKKNGQHVIYHLEESKKNITKNLFKLLNI"
     gene            2614..3837
                     /locus_tag="PKI16_00970"
     CDS             2614..3837
                     /locus_tag="PKI16_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR53044.1"
                     /translation="MNKKLLIVPTIIVGILLLLGGEYFLINKYVCKNTQTETSTPQAM
                     MLLIEFQNTDGLTNMINDMKERDVKGLLMVNGDFIEKHAEVLKEIMKTGVVELAPSYD
                     PEPFWGMSYDKQYEIILDMIENAQTYLGVTPRVISSRYMASDENTVKVAQTLGIEYIT
                     ARGTTELATTIYKPKEYDVKIISVSNIDVPEFKYGSFCDYSFYERNGSPEDMETQYKR
                     AIQNEKFMAVSHTYIGGYKERWNNMWHKFWDNYEVDWVDLDTLGSVDKEMPMWQIPVN
                     KNAPYTPEKIRPAISYEEEENVTNPCKVEDLNEGDSGIAESSNNGVVVFHNGTGPMCL
                     EMEEFFKENNIEYEEHLTTDTDFGTQLNAYKGDISKSEGTSESFGYYPIIFIGEKAYS
                     GFNEEIGEEILKILE"
     gene            3909..4211
                     /locus_tag="PKI16_00975"
     CDS             3909..4211
                     /locus_tag="PKI16_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HNR53045.1"
                     /translation="MKNFLNFIREQGVVGLAVGFILGGAVSKVVSSIVTDLINPLIGL
                     LLGKVGDLDQAFLQIGESKLMWGNFVSTTIDFIVIAFVVYFGVKILRVEKLDKKKD"
     gene            4477..4857
                     /locus_tag="PKI16_00980"
     CDS             4477..4857
                     /locus_tag="PKI16_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53046.1"
                     /translation="MEDKNNTNNGFLLGLSITLGTIVIGLISFLLYTNQVDKEPLRCE
                     YNGWAYADGEIYDSSDGCDTCFCNDGKTICTDNVCDAKINVKTCTYEGKTYKEGEGFK
                     SSDGCNTCGCMDGEVTCTLMACDK"
     gene            4860..5474
                     /locus_tag="PKI16_00985"
     CDS             4860..5474
                     /locus_tag="PKI16_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNR53047.1"
                     /translation="MNEIFERTCCRVFDDSKTISKEDLTLIMKAGQAAPSAKNRQPYF
                     FVAILNKKCREEISAVALKGRQKQFSNLSPEEFKKIEKGDTGSNDISIANASAAILVF
                     RNSDPEYSESKVESKNLNIKEEQGVATAAYSMMLQAQSMGISSGWICSPLYIKEELKK
                     ILTKYRVKFDNNWEPRLIIPLGYPSFTTKKPPRKSLEERCVFVE"
     gene            5549..6073
                     /locus_tag="PKI16_00990"
     CDS             5549..6073
                     /locus_tag="PKI16_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53048.1"
                     /translation="MGRFNQGRNNFSGRREDRPEMFDAVCDECGKDCKVPFRPTGNKP
                     IYCSQCFEKKGGPSRDDGRGRRSFDRGSNRDFGGRRDEKPTMYKAVCDECGESCEVPF
                     SPSPDKPIYCSRCFETKGGKGQKGGDNTALIEKLDSITEKLDKILLALEPKVVAKKVA
                     VKKTVAKKVVKKKE"
     gene            6571..7284
                     /locus_tag="PKI16_00995"
     CDS             6571..7284
                     /locus_tag="PKI16_00995"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNR53049.1"
                     /translation="MQTKYNESKLSEEYLESVRAEGATDSDIESWWNLTEVERALIIK
                     DDIVSRMAVFIDAKTKGLSDKKAMDEVKKAFPIFDIDFYKKNKEDGQNRPLPYELKDK
                     INLHMLELANEIGLEKLKKEVRKYPTFNSYIRNFIEREHLENQTSKSTTHNNNLRQET
                     TQTTKKNSLAIISFILSILAPFTGFSLLGILGLFLGILSLNQIKVSNENGKGLAITAI
                     IIGSIWGVLKLIVDVLLLL"
     gene            7321..7998
                     /locus_tag="PKI16_01000"
     CDS             7321..7998
                     /locus_tag="PKI16_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53050.1"
                     /translation="MRKDYKGIAENDVLAQLCIYCVRKSYDIDDEYTPILFLGWGKYT
                     NSMQMLGLRLLKNAVSQTDLVQNKASFLTNLTTDSTIDIKLYRKWVEEGALKDRILDL
                     KSLKGEEINYNAIITLATNHAYYLGQKTKETAEILNKNLSFVLLLEIDFVELEEYQKE
                     ILVNSKLTNDQTLYIQKGLHLLRNLYFSEMFIKYRKEDEETKIYFKDIYRKILEEMEN
                     DFQNKSR"
     gene            complement(8051..8473)
                     /gene="ssrA"
                     /locus_tag="PKI16_01005"
     tmRNA           complement(8051..8473)
                     /gene="ssrA"
                     /locus_tag="PKI16_01005"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8520..9248)
                     /locus_tag="PKI16_01010"
     CDS             complement(8520..9248)
                     /locus_tag="PKI16_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNR53051.1"
                     /translation="MEIFSPKLKKYQRKFGYSYALGTYPTIDLLKYKSGSVLKVLLNS
                     HHDGSEGVQEVQRLCRELGIKIEEQDRAIEKIAFKENTYTVGVFKKYVCELEKGKNHL
                     VLVEPRNMGNLGTIIRTMLGFNVRDLAIIRPAADIFNPKVVRSTMGAIFQINFKYYDT
                     LESYIKEFGNHHIYTFMLNGAKDIREVEFEEPFSMIQGNESRGLPESVKNLGESVYIP
                     QSKDIDSLNLSVATAIGLWESSKK"
     gene            complement(9250..10200)
                     /locus_tag="PKI16_01015"
     CDS             complement(9250..10200)
                     /locus_tag="PKI16_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53052.1"
                     /translation="MKPKRLKEEHIDMQFLALLIAVLAFLIFALFIGDIIWIKDFQYF
                     ISFLSGIMALFIGTLALLRYYTKKTNLNFLFLGGGFVGVGLIEILQIMLEVEDFQKLF
                     TFTESGSYPLSSALAKAFLATLFLISWFVIRKRKGRGRKRELQLMGVVCLTFILFVAT
                     FIILLLRGVEVDSFTVVLIGLFSLLLYILSLLGYLFNKGWLYDDFHYWIIFTLSFLIL
                     SQIFYLPFLNIEYIHMINLSTWAHFIAYVGMLVGFLNSIYMMYQKEKEIQQELEEKNL
                     LLDETKAKVEEAYLTLRKEKWELVNKKGGLGSILKEISKK"
     gene            complement(10215..10736)
                     /locus_tag="PKI16_01020"
     CDS             complement(10215..10736)
                     /locus_tag="PKI16_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53053.1"
                     /translation="MNAPNSGLNFLPKEEEEEEVKKGFSVLDFLVILCALFVIAFLGY
                     LIINPQKEGAENRNVVRNADVSLILSAVASYTKKTGEIPDVIPIGEECVKLGNEICKM
                     GPYDCVGMVDLSVLNSKDEGEEIVASIPSDPTNKSTNGTGYYITQDGSGTVTVCAPYA
                     ERGELISFEKYLY"
     gene            complement(10769..11014)
                     /locus_tag="PKI16_01025"
     CDS             complement(10769..11014)
                     /locus_tag="PKI16_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HNR53054.1"
                     /translation="MEAFLGILTLGFAWFWILVILLYLCSIAFSIWMLIDCIRREESN
                     FKDKTLWTVLLAVGLFLGYSLIISVVYYFVVKRELDK"
     gene            complement(11200..11286)
                     /locus_tag="PKI16_01030"
     tRNA            complement(11200..11286)
                     /locus_tag="PKI16_01030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11250..11252),aa:Ser,seq:cga)
     gene            complement(11286..11981)
                     /locus_tag="PKI16_01035"
     CDS             complement(11286..11981)
                     /locus_tag="PKI16_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53055.1"
                     /translation="MVKKENNKFQDELEQLSQIISGEVGLRKREESIKKAKEEAVENQ
                     KNIRRKNIVKGKTKLEQDPIFEDEKPVVQNIKLFEWEAPDRYEFKFSSKPFLIIVILS
                     LFFILLLAILRKYFLMGSIIALLFVIYVLGTTKPQIVKHKITARGFDTGGKLYEWYLL
                     KNFYFTKKGDQLMLLVETSLRFPAMLIFLVDEKDKEAIFVLLQDKLLYKDIRKQGSLE
                     KINYGEYIPLEKI"
     gene            complement(11989..12357)
                     /locus_tag="PKI16_01040"
     CDS             complement(11989..12357)
                     /locus_tag="PKI16_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53056.1"
                     /translation="MKCVFSLVSVIIVSVGFLFFLFNNPYFVPTNGVGEIEWLNIFVL
                     LFFLFIAVFCAISLAVFGTMTLLRKDLKRRMRIFIASKVALFFSLGLLLVFLLNFFHV
                     LDWIWGISILLVVLVASFVI"
     gene            12436..13113
                     /locus_tag="PKI16_01045"
     CDS             12436..13113
                     /locus_tag="PKI16_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR53057.1"
                     /translation="MNILIIENSNTLAHLLQKTLDSYGYNTSIDSSSFINKNLVKGNI
                     FDFVILNINLEKGISYEILAYIRKYSPNTKILGVCNKGSWVEKVEFLNKGGDDVLTYP
                     FPMQEVLARIQSLIRRPKSYLDESIYIGSYVLDTKGKAVLKDEKEINLRKKEFELFEY
                     LVRNKNRTVSRCELLDHVWDYRQYVGSNTVDVHIKRLREKLQDKNLIGTVHGIGYQVK
                     DKKPKAS"
     gene            complement(13100..14218)
                     /gene="dnaN"
                     /locus_tag="PKI16_01050"
     CDS             complement(13100..14218)
                     /gene="dnaN"
                     /locus_tag="PKI16_01050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNR53058.1"
                     /translation="MQFSCNQDTFSKYINIVSRIVNNKPGLPILNNVAFETVKGKLLM
                     KTTDLELSVSTWIGSDVKSEGKITVPAKQLAEFVNSISEEKIDFEVEKQSFNISTTNN
                     SANFNTMPFDDFPSIPEIKDEKDYFVRINKGDFANAVNRVAFAAATDDIKPVLTGIKM
                     EIEGDSVSFVATDGLRLSRQIVKIEKSGKQKSLLIPVRAFEEISHIVSELDSEDEWVK
                     VYVIEDRNQVLFKFSDVELSSRLIDGEFPEYKQIIPTGFKTQCEINRMDFLNSLKVIN
                     IIARSVLGNKMILDIAKEGVSLSAMQSDLGKNQSRFDCKVEGEDLKIAFSAKFLADIL
                     NHIDSEDIIFECSEPVRPGVFKIKGDENFVHLVMPMML"
     gene            complement(14671..16137)
                     /gene="dnaA"
                     /locus_tag="PKI16_01055"
     CDS             complement(14671..16137)
                     /gene="dnaA"
                     /locus_tag="PKI16_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNR53059.1"
                     /translation="MDKADFAEHVEKLPKPEILNSHAQKTQTDPDELWKSAKENLRLI
                     IGPKVFGGIFGGCYIENIQNGLVQISCDASYKREKILRDYKATLKKALLKSSGRNFEI
                     EINLREKDTFPKEKYLYHDPSQKEDISLDLFSSVEKEQKQKENALKNAQLNPKYLFSN
                     FIVGSNNRLAEAIAEAVVEDLGNAYNPVFYYGDTGVGKTHLMQAIGNEVLNRDASKKV
                     VYISIEQFLNEMVEAIRTKRNEDFRNKYRAVDLLMIDDVQFVETYPRTQEELFHTFNT
                     LYQANKQIILASDRPPKEIKNITDRLRSRFQGGMVADIQAPDYETRMAILKQYLLEKN
                     SSVEVPEEFMQMIAKNIDKNVRELEGSLTKVLSLIKLGMNPTEDDIAKLLQIDLESKR
                     KKITPMKVIKAVSDVFDVKISEIKGDRRTAYIAQCRQIVMYILRNELSMPLERVAKEV
                     NRNDHTTVLHACEKIDLLKKKDPKLNERLNECYKIIRD"
     gene            16390..16572
                     /gene="rpmH"
                     /locus_tag="PKI16_01060"
     CDS             16390..16572
                     /gene="rpmH"
                     /locus_tag="PKI16_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNR53060.1"
                     /translation="MKRTYQPKNLKRIRKFGFMERNSTKAGKDVLKRRRAKGRVKLSA
                     SEEYSLLRKKPTQSIR"
     gene            16577..16915
                     /gene="rnpA"
                     /locus_tag="PKI16_01065"
     CDS             16577..16915
                     /gene="rnpA"
                     /locus_tag="PKI16_01065"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNR53061.1"
                     /translation="MLNKKYRLSVNQFPVVYKNGKKIRGKYGMLIALKVENSSTPQFG
                     FVVSKKIGGAVQRHRFTRILRVAVAESIKEMELDNCGYIFQYVAFEFCDSKDVLKKEL
                     QNQLKGILND"
     gene            16908..17150
                     /gene="yidD"
                     /locus_tag="PKI16_01070"
     CDS             16908..17150
                     /gene="yidD"
                     /locus_tag="PKI16_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013242975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNR53062.1"
                     /translation="MIKKGVLFLIKIYQALFSLDHGLLGMLFPNTRYCKFIPTCSEYG
                     YEAVEKYGVIKGSILAIKRVLRCNPWTKEIGPDPVP"
     gene            17266..18204
                     /locus_tag="PKI16_01075"
     CDS             17266..18204
                     /locus_tag="PKI16_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNR53063.1"
                     /translation="MVSFYHLFGDNLGWAIIAIAIILRLILIPFTKSQANMTKKMSSL
                     KPQLEELQKKYANNKEKLSQEQVKLYKKVGYNPLGCLVSFIPQLIILSVLIAVLRAVT
                     NNTFEGLYPFVKEWAFGASDVFISTKFLVWDLARQLNGNVDGEQLNQISEFMKYSPLQ
                     IIELFKDGTISRETMAIISKYGISNQYGLTSIHSIYYMILIALVGISQFFTSKFTQAI
                     QNPNGITAPTPQKSKKGDVPSMESMQKQMTKSMNFLLPAMTVFFAISYPAALSFYWTV
                     QSVMLIVQYFILDFDKTKVGVQNLFTKMVDKAKKRR"
     gene            18249..18731
                     /locus_tag="PKI16_01080"
     CDS             18249..18731
                     /locus_tag="PKI16_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNR53064.1"
                     /translation="MKDEDIVKLVNKEVDKLCKLLEVDCERDCQIEDSDTGVKYIKIS
                     FEGQDLGYMIGSRGRHMDSLQFILQVIIGRQLDEGTDFRVFVDVGDYRKAKDESLEQM
                     ALQKADDARLLGEEIELPPMKPSDRRVIHLTLDKFKDIKTESRGEDRDRHIVIIPIKE
                     "
     gene            18754..20145
                     /locus_tag="PKI16_01085"
     CDS             18754..20145
                     /locus_tag="PKI16_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNR53065.1"
                     /translation="MKKISKDYIFLGIVILFSILVSLLFCSENPLRLITILSSFVVLF
                     CFYFFSKNIFFSSFLYILITLPFNITFQIPQSVQIFGNDILLYNPYVEGVYVNYLVPT
                     LSILDLGVILLLLAPLLHNGWGRFVDIFKRYGIVLPLFVVFLLLQNVLLNDPLSFLNS
                     ARLILYILGFLFLLEFFKEGKKKYSEPLLVVSVISVLIQGIIGILQFTRGSSLGLGFF
                     GESQVVSGMQGSSFVTLGGEVFLRAYGTFPHPNLLAGYLLFNILLALTFINHEKGRFK
                     ILPYILLLLSLTSLLFTFSRVSILLAVLVLIVYFGAKIVNGKLFAFSLPLFFERFVNL
                     FSSGDTSWEDRVKLARSSLRVIKENWLSGIGIGNFVKGIEGDVPRSVNDVLLLQPVHN
                     FFLLTFSELGLIGFGLYMWLLVDVCRKNIVKITLFGVLVLVCIFVLISFDHYTFSLAQ
                     GIMLFFLLLLLLF"
     gene            complement(20200..20289)
                     /locus_tag="PKI16_01090"
     tRNA            complement(20200..20289)
                     /locus_tag="PKI16_01090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20253..20255),aa:Ser,seq:gga)
     gene            complement(20293..20369)
                     /locus_tag="PKI16_01095"
     tRNA            complement(20293..20369)
                     /locus_tag="PKI16_01095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20333..20335),aa:Arg,seq:tct)
     gene            complement(20416..22248)
                     /locus_tag="PKI16_01100"
     CDS             complement(20416..22248)
                     /locus_tag="PKI16_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53066.1"
                     /translation="MDEIKKNTKLVIGRDDELTDVVTGILEAETERVILTFADESDLL
                     ISPINLSVLQETADEAEKVLVAQIIKNPTGLRNSSIAGLTTTDSPNNPTEEMWIEAEE
                     KKIARLAPKPKDKPLEKKEELPEEKSASTFEKKINDAIQRSREGIATKAVEKNVQKEG
                     VFLALDEDLPTEAPENIEDVPIKFEDFPEETITPKPLLKKGFKLPTVNFKAFWPFKKG
                     TGISTPTGIKKYLPIGILSLILVTILAAIIYIQTVPFVKVAVYVESKEVSIEKIFVGD
                     ENIKQIDFDNLKIPIKVESVEKDRSSTIKATGKAYTGEKATGKVQLVGGPSCTEAIAL
                     PAGQILLSSDKAFTMDTAITVPCPGIVEVSVHARDFGTEFNIESNSNFLAQGHLDRQL
                     FGYNPSAFTGGTKEEYTVLSQSDVNSGIEKLKSEAELEAENELRDASNEWSIIEDSIK
                     SEVVKDSIKTSVAVGAAGSDVDLSLKIKSSATYYLTEGFDDGISALLTKEAQEKNLFE
                     GDNDLELGLSNSIEKTISVVEDSKKGVKIKLVAKGDVSPKVNKEEIVNTLKEMKWEQG
                     LEYLQTLHFSEKRISASFFPKNILSKYPYFPKRQGGIIVEIYNM"
     gene            complement(22248..23495)
                     /locus_tag="PKI16_01105"
     CDS             complement(22248..23495)
                     /locus_tag="PKI16_01105"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNR53067.1"
                     /translation="MLGLPFLKNSSKNLLDGTTNLIALDIGTEQLKSILFSMGDIGID
                     IKKISRIDQQQNAMSKGIITNLDTVLDNCRLSISELTSRLADEELPKNVVMGIAGEYI
                     QGVSIIVNYQREENYDREVTQKEQNKIIGQIKAKIETNGKEDLGQRTGLRNEDIDILH
                     ITHTGLEIGGMPVDSLIGYKGREVKLHFYASFAPKTYTEALRKVAQELGLEVIGIVSQ
                     PFSVARAFSGSSNRDFSAIFIDIGGGTTDIAVVDKGNVVETKMFAFGGRTFTKEIAKV
                     LQIDIRHAEQRKIKYAEKELTRELSNTIQKIVYPVSKLWMKTLKTALASCDDIDSFPP
                     QIYLCGGGALLPEMKGAMLEFPWKKYLPFTVVPRIEFFKPDRLDKIVDNSGELKHVFD
                     ITPASLTKFAYDLEISKNRNIKI"
     gene            complement(23525..25207)
                     /locus_tag="PKI16_01110"
     CDS             complement(23525..25207)
                     /locus_tag="PKI16_01110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNR53068.1"
                     /translation="MKENSKEFLKLCTLSGTAEIGRNCNFVEYGDEIVMIDAGYAFPG
                     HEMYGIDYLIPNVSYLKKNKEKVKAILITHGHLDHTGALKWILPELGYPPVYAGGFAK
                     ALIEVKMKEYKMENKVKIISVDRKSTINIGRNFKATFIGINHSIPDAFSIFLESPRGN
                     MFFSGDYKIDMSPANEPQTDYEKLRQLRGKIDLALMESTNASNPGKAPSETEIFQNLG
                     EIISQQKGRVIVAAFSSLITRLYSLVEVAKRTNRKIVLSGKSLEQSIDIARQKNYIKI
                     DDNLIVKERNMSKYPDNELLLLCTGSQAERFAALNRISLNEHKFIKIKKGDFVIMSSS
                     EIPENISSIERMTDRLIGLGADLIKDSVELKIHSTGHGNQEDMKMMYNLIQPKYIMPI
                     HGPLTFRYFNKQNFVRWGVDEKNIFLTDDGQTWLFNGYSWSRGKKIESKPILIDGLGV
                     GDIGDIVLKDRKQLSEYGIFAVILNISSKNKKIIGRPRFISRGFIYMKGSRELLQEIE
                     NLVMDIHRDWLNKSQREKVFKDEELRERIEKELSKLIYKRTEREPIILTAII"
     gene            25283..27523
                     /locus_tag="PKI16_01115"
     CDS             25283..27523
                     /locus_tag="PKI16_01115"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNR53069.1"
                     /translation="MLRDIPKTFESQDIEGRIFQMWEESKLANPEVVEKYFQEKGRDI
                     KEPFTIILPPPNANGNLHLGHMCGYSYHDVLARYMRMTNHPTLLVPGKDHAGIQTETA
                     FTKILESKGESKWDIGRDEFYKRCYDFCIENADNARRQEKRIGLSADYSREFFTLDPK
                     LTKIVYDTFYKMYEEGLVYRDKRIVNQCPNCKTALADIDTEHEERRGIFAYIRYPFVV
                     GNGKDGITVATTRPETMLGDTAVAVNPKDERYSKYIGKKLLLPIASREIPVIGDEEVD
                     MELGTGAVKVTPAHSPVDFEIGKKHGLEIENVIDSEGKMTGNIPERFKGMTTVECSKA
                     LCEELEDLGLLEKIENIKHEVSICERCKTPIEPIISNQWYLNVETLAQKALGAIKSEE
                     IKVIPFGQQKALEFFFENIQPWCISRQLWWGQRIPVWYNGGKELYDWLNENKGKSVAD
                     YENETGKKANGTGEIKLGEDNPGDGWEAETDVFDTWFSSGQLPYSTLGGPDGDDYKKY
                     YPTQIMIHARDIIFWWSARMIMMGLYRTNQVPFPTVFLTGMILAADGSKMSKSKGNGI
                     EPSEVFEKYGADSLRLWYYSDALPGSNAPIREEKIKGNRNFVNKIWNASRFILMNIDE
                     SEIEGIKNHEPLDTERISRTREHILSVSRYIEKYQFNLGAEAIREFFWHQLCDVWIEE
                     IKEEIKDQPIGSDIRIEKLSELLWILKNNLKIMHPFVPFVTEAVWQELVKIGLAEDLL
                     MGEEIK"
     gene            complement(27612..27752)
                     /locus_tag="PKI16_01120"
     CDS             complement(27612..27752)
                     /locus_tag="PKI16_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53070.1"
                     /translation="MGLFPNPYIGGICDKKKEKSVGKEETETLWENERKKIPLKKGVG
                     CE"
     gene            27833..29281
                     /locus_tag="PKI16_01125"
     CDS             27833..29281
                     /locus_tag="PKI16_01125"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNR53071.1"
                     /translation="MNKQKIYITTTLPYVNAEPHIGHALEFVQADALSRYFKNKLGKE
                     NVFFNVGTDEHGQKIFNKAKEEGLSLNDFVDKYAQRFKDFCKLFFVEYDNFYRTSTPE
                     HHDAAKTFWKKCEEKGDIYKKRYSGRYCVGCERYITEKELVDGKCPDHKTIPELKEEE
                     NYFFKLSTYREPLLKWLDENKDFLKPESKIPELRKIIAEIEDISISRLKENLPWGIEV
                     PDDPEQVLYVWFDALTNYVNAVGFGTDEKKLNEWWPAIQLCGPDNLRFQGAIWQGMLA
                     SVGLPHSSKLLVHGMVLGPDGTKMSKTIGNIISPFDQEEKFGAEIVRFYLLTGITTYG
                     DCAYKEDDLINMYNSRLANNFGNLLNRTIHLANKTAVNINNEQLVEEDFKKTVDTYVE
                     EVRRLYEDFEISLAGEKIDELADFGNKYITKEEPWTKEKEQTEKILNNLSYLLEKVIH
                     LYNPIIPQSASSAIQHLIARETVILFPKIESR"
     gene            29340..29897
                     /locus_tag="PKI16_01130"
     CDS             29340..29897
                     /locus_tag="PKI16_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53072.1"
                     /translation="MFKSEKQPKSIDFLQPVYSPTDIWSNAYIWLTEVGKYLLIAVEV
                     VVLGVFLSRFILDKRNNDLTEEVNSKVTLLSNDTWKGNAMLFDNYQSLLLDVKKVREE
                     QEINSTTISELINGVPSSLKIKNFSFSRGKVAFLFTANNLDAIKNYEAALKNNTDYHD
                     VRFSITKDSNEINVRVTFSLYEEKE"
     gene            29897..30514
                     /locus_tag="PKI16_01135"
     CDS             29897..30514
                     /locus_tag="PKI16_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53073.1"
                     /translation="MDEKNINTTEYIKIIKESPEKKKSYIFVAVTVAVAIVLVIFAIR
                     PTILTITRINKEIKDKQRIDEVLTKKIETLTELDKQYTQVKDSFSGLALIYPADANFS
                     LLLANIESVVSRNGYSLGSVGFDKYDKNDNYKINAKYLVPWSLRVTVKGRVGNIVNLL
                     KDFESMPMYPVVESLSYSSQVDKDGLTTFGINMRIYHIENSNFYE"
     gene            30519..30761
                     /locus_tag="PKI16_01140"
     CDS             30519..30761
                     /locus_tag="PKI16_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53074.1"
                     /translation="MEMLKKAIIALILLGVVVLAWVGGSIYYQNTNIEVSPNAQEYTK
                     TLRDSFDLEELEKITERTENSFPVSPSEFTVLIQGN"
     gene            complement(30758..31837)
                     /gene="rpoD"
                     /locus_tag="PKI16_01145"
     CDS             complement(30758..31837)
                     /gene="rpoD"
                     /locus_tag="PKI16_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNR53075.1"
                     /translation="MTDKSKAIKNLVDKGKDQGFLTQEDLLDIFPTIEEDVELLDDIF
                     KSLQENEIEVLEPEDTLEEVVKEELTLEKKIRILKTIQSSLSTDAIRSYLYEIGRIPL
                     LTGEEEVILAKRIENGDKEAGQLLITANLRLVVSIAKKYSKSGLELLDLIQEGNIGLM
                     RAVEKFDYKKGFKFSTYATWWIRQAITRAIADQARTIRVPVHMIETINKFNKVNNVLT
                     TKLGRPPLDEEIAKEMDIELSKVAEIRKIKQNPAPLSTPIGEEKDSKLQDIIPDDWSQ
                     SPEDFATAEYLKNQLKDILDTLQDRERRVLSLRFGLDDGVSRTLEEVGKEFGVTRERI
                     RQIEAKALKKLKEKSAQQKLDDYID"
     gene            31863..32201
                     /locus_tag="PKI16_01150"
     CDS             31863..32201
                     /locus_tag="PKI16_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53076.1"
                     /translation="MDSKKDDLNDKKQFFIDTIARFCDKCGSPFVIEDVNILQRTGIS
                     SIIHFSCHNCKSKHIATFISPIGMSHRVPVNTDLSVKEISKFAQQSETSTQEILDIYM
                     YLKKNSKVLI"
     gene            complement(32198..35188)
                     /locus_tag="PKI16_01155"
     CDS             complement(32198..35188)
                     /locus_tag="PKI16_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HNR53077.1"
                     /translation="MSNSALTKIKSQTPVTVIVHGGSRLGYLITKTLIEQGSHVIIID
                     KFNAQTKEYITELKKSKSVDFFEFKGMEALFKNLKRFDYLFYLLNEQLSQKDFDSKEF
                     LRETGYLDLTLRNARKNNAKFSLITSLALNRELSNRVNNSKVASPSPYSNIELQKYCE
                     TLAAEFKDKTNLNIRILRIGNLLGKGINRVDNDTLHNLLKDATEKPQITIKGEGLDLH
                     NVINENDATYGILKLTFSDKTRGEVITLANKNNYTTLSIAYKLLELNTEAQSIKFTES
                     ENTEFVIQDLYVPAPHATKYGWIQQVTLEDSLIEQIQTYYDSINKKWDVEGKQRLEKK
                     EKSNVKIYKTSLGNFVSKLFTPIKNIFAKKDFDSSQISKYVGISILTVLFTYFFVYPV
                     IGTLIGLTIISSSAKTLSNSLLDLETSKAKKEITVLDKNIDRVSGTLSNLKWVFQITG
                     GTDLYNNSVELIVGAQYAVDGASGLLDSIEPLASYIKDFQPAVDIQGSLPSTSREYRE
                     YLIAIKENQYKIDDATYKISLSNDIVNSVNTSVFPTFLQDKILELKDLVEKLDSTTSD
                     YKEIITFLPDLLGVDGRQRYLILLQNESEIRSTGGWLTSYGVVGIESGQVRELFVDDI
                     YNADGTLKVQGKTFNAPKSMNQALGLTTWNFSLVNWYPDLTETYLNAEPFIRALGKGD
                     DLDGIITIDITFIQKLLSKWGGIEVPGETELVTSDNLYDKIFKLHENFTPGTTQKTTF
                     LANLANEIIQKLLSKNISDLISLSDVFGESLDEKHLQASFKNRDAFNFFNSRAWADSL
                     DSKYNEAPIAIDWNWGANKANLYLEKNYNLQVDIKDEDTIVFKYTIATENTSENLVYP
                     QGNYINYQRVYVPSYATIISVKGMDDNKYTTYKESGFKVLGGWFNTNIKDVNTLEITY
                     QLKRTPENASFPIVKNGDNIFLAIDLFKQPGEKSHAYKLDIIYPSSWNIENAGNLNAI
                     QNQLSSRFELNKDTSFDIVWRETN"
     gene            complement(35181..35834)
                     /locus_tag="PKI16_01160"
     CDS             complement(35181..35834)
                     /locus_tag="PKI16_01160"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNR53078.1"
                     /translation="MRNHKTAIIIVGSILLLSILFYFIPINRIIGSLPVIKRFYNNTT
                     LEIVLQNGKAKIWIDGKEYGETPQEIENLREGEYTIELEKIASGETFYKKQTFKIPLY
                     KNTSSRIDLEIGPENILHGSIMYYSPEPKISASEGFLTVTSNAQKAKLYIDGEYLNDL
                     PITEVKLKENQYQIKVIALGYMDVEVPVIIRKGYLLNLKVFNFPIPVNFDNVEDTNE"
     gene            complement(35878..>36752)
                     /locus_tag="PKI16_01165"
     CDS             complement(35878..>36752)
                     /locus_tag="PKI16_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53079.1"
                     /translation="ILIALGILIVGSFLDDILNLPAKVQFLYQILAALVISFSIINLT
                     NISFFNIELQSISWDFSIFHLQQSFVFPGDLILFVWILMCINAVKWTAGSPGIVEANS
                     LIIFALIFVIALRENAIFSSTLSILAVGILLVLVMFAFPPQKIMSGSSGKTVYGFLIC
                     ILAIIADSKMSTTLMLLTLPLIDFIYVIVKRIFVVKPKNLLTLLRINDTNHLHHQLLK
                     LNLSRRQVVLLEMSATLLLGSFAILTTGAIRYFALILGVSLGIAFVVFINIKASRKRI
                     GNEDEKSPESKYSY"
BASE COUNT        11741 a         6337 c         6476 g        12198 t
ORIGIN      
        1 tcatcttgta atctggagaa gtgtttaatt agatcgttga gaaattttgt tggattccct
       61 tttggcttga gagtatctaa tgggaggtca tagagaaatc ttcgaagaaa gatgtacgac
      121 tgtgctccac tcatcaaaga gtaattgctg tctagcccaa tatggtatcc gtcctctcgt
      181 aggactctgt cacaaaagga gtgaaatgta gatatccagg gttcttcgta tccatactcc
      241 atctccacat ctactctttc cttcatctct tcagatgctt tttctgtaaa tgttagtgca
      301 agaatttcag agggttttgc caaatctttc ttaatgatat ttatgatcct ctgtgtgatt
      361 acagctgttt tgcctgttcc tgctcctgca attatgagga ggtctccttt gttgtactct
      421 actgctttct tctgattttc gtttaatcta atttgtgaca ctatctcagt ggtagaagta
      481 agaacacgag gtaaagaatc aaggcaagaa ttatccctga gaggatagca aacagtaatc
      541 ttccatttgg gaatttgttt aaaaattttg taagtttttc cttgttcatg aatatattaa
      601 ttaatttaat ttgctaattt tttaagaacc tctacttctt tgtaagtatc tgtgtcattc
      661 tttaatgcag gggtctcgag gatgaaggga atatttgcaa gttttttgtt atgtaatata
      721 tctttaattg ttttctcccc tatctttccc tttcctaaat ctgcatgcct gtctttgtta
      781 ctaccaagtg cagttgcaga atcgttaaga tggattgcct ttacattctc aataccaatt
      841 tctctgtcca tgtcgcttaa tactccatta atgtcattaa tccagtcata tccccctgca
      901 aatgtatgtt gggtatctac gacaaagcct acccttttct tgtctttcac tccttctcgc
      961 ataatcttaa gttctgtaaa tgttcttccg aggttacttc ctgtacctgc agtgctttca
     1021 attagtagct tctgtgttcc aggtacttcg tccaaaaccc actgcattcc gtaagatatc
     1081 ctctccagac tatcttcgta ggagagttct ttttgggatc caggatggaa gcatactcca
     1141 agtatttcac tgtttatttt tcttttttta atttcttttg ctactttttc tgcaaaatct
     1201 aagtaaacca ctaaacccat tttccctaga tggaacatct gtttgtcgct tcttgccagg
     1261 ttagttaaat atattccgtg gattaatatt tttttaatat ttgaattttc taattcatta
     1321 acaaattttt caatttcttc atctgaaatt aactttgtag cccatctcat tggtgcggtt
     1381 ggcataatct gaatgctgtt tacaccatac ttttctccat tttttattga attttgtata
     1441 ccaccactag atgatacatg tatgcctaag tacatagtat attctagcac agatagtgta
     1501 taattaggac cattggagag gtgactgagt ggtcgaaagt acctcactgc taacgaggca
     1561 taggccttaa aacctatcgc gggttcgaat cccgccctct ccgtattaca aaacatatca
     1621 ttccgaacac cttcaaaaca ttcttttttc tcatatttca atcagtttgg gatattcatt
     1681 caggattaga tagtgaacgg aacatccgaa ctattgactt tctatttgta agagatatat
     1741 acttagttat gggtatatac ccattattaa atttttatta tatttatatg ccaaacttag
     1801 acaatacagg cccattaggc caaggcccta tgacaggaag aagattagga tttcttccaa
     1861 gatttagagg aaaaagatta ggtggctcac aagtatgtaa atgtccaaac tgtggatata
     1921 cagaaccaca cacaagagga ataccatgta caaaaaaaat gtgtccaaaa tgtggtactc
     1981 cactaagagg agaatactgc taatatgaga cccaggttta gaagaagact cggaatacaa
     2041 tttaaagata catacttcaa accacgagga atccccctta gagagcttag cgaggttaat
     2101 gttacagatg aggagttaga aacactcaga ttaagatata tagagcacat agatcaagaa
     2161 caagcagcta agatgatggg tatatctcaa agccagtatc aaagagatct tacctctgtt
     2221 atggagaaaa ttacacaatc cttagtgtat ggatatgcca tctccataac taaaagagag
     2281 tcatagtatt tcagccttga ctgagatata tactcttggt atatttcaat atatgttgaa
     2341 acatattgaa caagaaaaca taaaagaatt agagaagcta ctcgttccta atcgtattca
     2401 aatactccac ctactctaca agaaagacac ctgtgtatgt caaatggtta aaaaactaga
     2461 attaaagcac aacctcatat cccatcacct caagacacta caagagatgg ggtatatatc
     2521 tagtaaaaag aatggccaac atgtcatata tcatctagaa gaatctaaaa aaaatatcac
     2581 taaaaatctt tttaaattat taaacattta aaaatgaaca aaaagctact catagtccca
     2641 accattatag ttgggatact actcctccta ggaggagaat atttcctcat taacaaatat
     2701 gtctgcaaga atacacagac agagacctcg actccacaag ccatgatgtt actcatagag
     2761 ttccagaata ctgatggttt gacaaatatg attaacgata tgaaagagcg agatgttaaa
     2821 ggattactca tggtgaacgg cgacttcata gagaaacacg cagaagttct caaggagatt
     2881 atgaagacag gagttgtaga actggcacca tcatatgatc cagagccatt ctggggaatg
     2941 tcatatgaca agcagtatga aataatcttg gatatgattg agaatgcaca gacataccta
     3001 ggagtaacac caagggtcat tagttctaga tatatggcat cggatgagaa tacggtaaag
     3061 gttgctcaga cattaggtat tgaatatatt actgcaaggg gtacaacaga gttagcaact
     3121 accatataca aaccaaagga atatgatgtg aagataattt cggtatcaaa tattgatgta
     3181 cctgagttta aatatggtag tttctgtgat tacagcttct atgaaagaaa cggatctcca
     3241 gaagatatgg agacacagta caaaagagca atacagaatg aaaagttcat ggctgtttct
     3301 cacacatata ttggtggata caaagagaga tggaataata tgtggcataa attctgggat
     3361 aactatgagg tggattgggt agatttagat actttgggaa gcgtagacaa agagatgcct
     3421 atgtggcaga tacctgtgaa caagaatgct ccatatactc cggagaagat tagacctgca
     3481 atatcatatg aagaggaaga gaatgtgacc aatccatgta aggtagaaga tcttaatgag
     3541 ggagattctg gtattgcaga gagtagtaac aatggtgtag tagttttcca caacgggaca
     3601 ggacctatgt gtttggagat ggaggaattt tttaaagaaa acaatattga atatgaagag
     3661 catttaacaa cagatacaga ttttggtaca caattaaatg catacaaggg ggatatctca
     3721 aagagtgaag gtacttcaga aagttttggt tactacccta ttatcttcat aggcgagaaa
     3781 gcatattctg ggtttaatga agagattgga gaagaaatat taaagatatt ggaatagtgt
     3841 gagaaaatat acaattcttt cggaagtcat tccatacctt ccgccatata aaatttcata
     3901 ggttaattat gaaaaatttc ttaaacttca ttcgtgaaca aggagtcgtt ggtttagccg
     3961 taggctttat ccttggtggt gcagtctcca aagtcgtctc ctccatagta acagacctca
     4021 tcaatccatt aattggtctc ttgctcggta aagttggaga cctagatcaa gcatttttgc
     4081 aaattggcga atccaagctc atgtggggaa acttcgtaag tacaacaatc gacttcatag
     4141 taatagcctt cgtcgtttac ttcggagtaa aaattcttag ggtagaaaaa ctagacaaga
     4201 agaaagatta atccttcttt cttcctctag ttacaatcca gtgagttatt cctgcaatta
     4261 gaagaatact ccattcttta aagaattctt tttttaaaga ttatgaggac aagaataatc
     4321 atatccatag caaaacggtt cacaaagagg ttcacaagag gtattgcaat acagataaag
     4381 atctattttc tggataaaag agaacactat tctagaaaaa ggattatatt tggaaatgta
     4441 ttcccatttt acagataaat aatatatact tttcgaatgg aagataaaaa taatacaaac
     4501 aatggatttc ttcttggcct aagcataacc cttggaacaa tagtaattgg cttaattagc
     4561 tttcttctct acacaaatca agtagacaag gaaccactga gatgtgaata caatggttgg
     4621 gcatatgcag atggagaaat ctatgattca agtgatggtt gtgatacatg cttctgtaat
     4681 gatggtaaaa caatatgcac agataatgtt tgcgatgcaa aaatcaatgt aaaaacatgc
     4741 acttacgaag gtaaaacata taaggaaggg gaaggattta aatctagtga tggatgtaat
     4801 acatgtgggt gtatggacgg agaggtaact tgtacattaa tggcatgcga taaataaata
     4861 tgaatgagat ttttgagaga acatgttgta gagtttttga cgattcaaaa acaatttcaa
     4921 aggaggattt aaccttgatt atgaaagccg gacaggcagc tccatctgca aaaaacagac
     4981 agccctactt ctttgtcgcc atattgaata aaaaatgcag agaagaaatc tccgctgttg
     5041 cactcaaggg tagacaaaaa caattctcaa atctttcacc cgaagagttt aagaaaattg
     5101 aaaaaggaga tacaggctcc aacgatatat ccattgccaa tgcttctgca gcaatccttg
     5161 tttttaggaa ttctgaccca gagtacagtg aatctaaagt ggaatcgaag aatctaaata
     5221 ttaaggagga acagggagtt gctaccgctg cctactctat gatgcttcaa gctcaatcaa
     5281 tgggaatatc ttctggatgg atttgttctc ctctgtacat caaggaagag ttaaagaaaa
     5341 ttcttacaaa atacagagtt aagtttgata acaattggga acctcgttta attatccctc
     5401 ttggatatcc atcgtttaca acaaagaaac caccgcgaaa atccttagaa gagaggtgtg
     5461 tctttgttga ataattttcc cttcattgct atcttccctt taaaaagcta ttatgcttaa
     5521 tatatttaga ttttaaggta taaaacaaat gggaagattt aatcaaggaa gaaataattt
     5581 cagtggtaga agagaagaca gaccagagat gtttgatgct gtttgtgatg aatgtggaaa
     5641 ggattgtaaa gttccattta gacctactgg aaataaacca atatattgta gtcaatgttt
     5701 tgaaaagaag ggtggaccta gtagagatga tggaagagga agaagatctt ttgacagagg
     5761 cagtaacaga gattttggag gacgaagaga tgagaaacca acaatgtaca aggcagtttg
     5821 tgatgagtgt ggagagtcgt gtgaggttcc atttagtcca tctcctgaca aacctattta
     5881 ctgtagtaga tgttttgaga caaagggtgg caaaggacaa aagggtggag ataatacagc
     5941 tttaatagaa aagttagata gtattacaga aaaactagat aagattcttt tagcgttaga
     6001 accaaaggtt gtagcgaaga aggtagctgt taaaaaaaca gttgcaaaga aggtggttaa
     6061 gaagaaagaa tagtacttac taagtagcca tgacttgcaa cacatcttca aaacactagt
     6121 tgctttctta caaaaagaca ccccccctgt attgaacacc ccttcaaaaa acttgtctta
     6181 acaagatttc ttatgtagcc atctttcaca agccgctttg aattccttta gcgctccttg
     6241 catattcttt gcaaccaaag gctttctcca aatgtaatta gcccccctcc ccttctcgtc
     6301 tagaaatgtt gagaatgaga aataaaactc accaggattc ccatataaac gcaaggattc
     6361 tatgccattc cttttaagaa aactgtcttt attatgtgtt atagttttaa ccattttgct
     6421 attccttaaa attaaactcg caaattaaaa aaagtatcct ttgactctgc ttatcaagca
     6481 agatatccct ccttttccac ctataatacc attttaagtg ctttctcgta agccttctat
     6541 catgcctatg tatgtgctaa aataacttta atgcaaacca aatataatga aagtaaatta
     6601 tccgaagagt atctagaaag tgttagggct gagggagcaa cggattctga tatagagagc
     6661 tggtggaatc ttacagaagt agaaagagct ttaatcataa aagatgatat agtgagtagg
     6721 atggctgttt ttatagatgc taaaaccaaa ggcctctcag acaaaaaagc aatggatgaa
     6781 gttaaaaagg ccttcccaat attcgatata gacttctata aaaaaaacaa agaagatggt
     6841 caaaacagac ctcttcctta tgaattaaaa gataagataa atcttcatat gctggaactg
     6901 gcaaatgaga taggtttaga gaaattaaag aaagaagtaa gaaaataccc aacatttaac
     6961 tcctacataa gaaattttat agaaagagaa catctagaaa atcaaacttc taaatcaacc
     7021 actcacaata acaacttaag acaggaaaca acacagacta caaagaagaa ttctctagct
     7081 attatctctt ttattctttc tattcttgca ccattcacag gtttctctct tctgggaatt
     7141 cttggcttgt ttttggggat actctctttg aatcagataa aagtatctaa tgaaaatggt
     7201 aaaggcttag ccatcacggc aattataatt ggttctattt ggggagtcct aaagctaatt
     7261 gttgatgttc tacttttact ctaactcttt ctaatttaac ttttacaact aaaatatatt
     7321 atgagaaagg attacaaggg gattgcagag aacgatgtac tagcacagct atgtatttat
     7381 tgtgtccgta aatcttatga tatcgatgac gagtacactc caatactttt tttaggttgg
     7441 ggaaaatata caaactctat gcagatgctt ggactccgac tcttaaaaaa cgctgtctca
     7501 caaacggatc ttgtacaaaa caaggcttcc ttcttaacaa atttaacaac agattcaaca
     7561 attgacatca aactctatag aaaatgggtt gaagaaggcg ctttaaagga cagaattctc
     7621 gatctcaaaa gcctcaaggg cgaagaaatc aattacaacg cgataattac cttagcaaca
     7681 aatcatgcgt attaccttgg gcaaaaaaca aaagaaaccg cagagatatt aaataaaaat
     7741 ctctcatttg ttttacttct ggaaatagat tttgtagagt tagaagaata tcaaaaagaa
     7801 atccttgtaa attcaaagct cactaacgac cagacgctat acatacaaaa gggcttgcac
     7861 ctattaagga acctttattt ctcagaaatg tttattaaat acagaaagga agatgaggaa
     7921 actaaaatat atttcaaaga catctatcgg aagattcttg aagagatgga gaacgatttc
     7981 caaaataaaa gccgctaagg cgaacccgaa agaagatctc tgacaaaaga ctgtactaat
     8041 ctctctcaaa tggtggagac ggtgggaatt gaacccacgt ccataagcat tgtctagtat
     8101 catgtacaaa catagttaac cctgtatagt tagtcaaatc taaaaggatt aacgaaaaga
     8161 tttaactact cagtaattat attcatcata tgctactcta ccgacaaagc agtactgaca
     8221 tatcctgata acgactgtct tagattccta atcaggagta gaaataaaag acattagcta
     8281 aactaatagc taaaccttat gcaaagttaa acgcataaac tggagttgca aaagcagctc
     8341 ttagtcttgc tgtaaaacca tctgcaaagg cgacaagatt attttttaca cttatccttg
     8401 tgtgttgatt tatgagacaa ccctcagttt gtgaccacta gacgcaaact cctgtcgaaa
     8461 gccttacgtc cccatattga aaaagagcat aggaatgtat tattcctgct ctcattatat
     8521 cattttttac tactttccca tagaccaata gctgtagcaa cagaaagatt taatgagtct
     8581 atatccttgg attgagggat atacacactc tcacctaaat tcttaacact ctctggtaag
     8641 cccctactct cattaccctg aatcatacta aaaggctctt caaactctac ctctctaata
     8701 tcctttgctc cattgagcat aaaggtatat atatgatgat taccaaactc tttaatatat
     8761 gattctaaag tatcgtaata tttaaaatta atctggaata ttgcccccat tgttgacctt
     8821 acaacctttg gattaaaaat atcagcagca ggcctaataa tagccaaatc ccttacatta
     8881 aaccctaaca ttgtacgaat tattgtcccc aggtttccca tattccttgg ttctaccaaa
     8941 accaaatgat tctttccctt ttctaattca cacacatact ttttaaacac ccctacagtg
     9001 tatgtattct ccttaaaagc aattttctct attgccctgt cttgttcctc tattttaatc
     9061 cccaactctc tacagagcct ctgaacctct tgcacaccct cactaccatc gtgatgcgaa
     9121 ttcaaaagca ctttcagtac actcccactt ttatatttca gtaaatctat agttggatat
     9181 gttcccaaag cataagaata accaaacttc ctttggtatt tttttaattt tggtgaaaat
     9241 atctccatac tatttcttgg aaatttcctt aaggatactt ccgagccctc ctttcttgtt
     9301 cactaactcc catttctctt tgcgaagagt aagatatgct tcttcgacct ttgcttttgt
     9361 ttcgtctaaa agaagatttt tctcctctaa ctcttgctgt atctctttct ccttctggta
     9421 catcatgtat atgctattaa ggaaaccaac taacatacca acataggcta tgaaatgcgc
     9481 ccatgtggag agatttatca tgtggatata ttcgatattt aaaaatggaa gatagaatat
     9541 ttgagataaa ataagaaagg ataaagtaaa aattatccaa taatgaaaat cgtcatacaa
     9601 ccacccttta ttaaacaaat atcccaacaa agaaagaatg taaagaagaa gagagaaaag
     9661 ccctatgagt acaacagtaa agctatctac ctctactccg cggagaagca aaattatgaa
     9721 tgtcgcgaca aaaagaataa atgtaagaca aacaacaccc attaactgaa gttccctttt
     9781 tcttcccctg cccttccttt tccttatgac aaaccaacta atcaagaaaa gagtggctag
     9841 aaaagctttc gctaaagcac tagagagagg atagctacca ctctctgtaa atgtaaaaag
     9901 tttctggaaa tcctctactt ctagcataat ttgtaaaatt tcaataagtc caacacctac
     9961 aaaaccacct ccaagaaaca gaaagtttag attagtcttc tttgtgtagt atcttagaag
    10021 agcaagtgtc cctataaaca gagccataat acctgagaga aaggagatga aatactggaa
    10081 atccttaatc caaataatat ccccaatgaa aagtgcgaag ataagaaaag caaggacagc
    10141 aatcaagagg gcaagaaact gcatatcaat atgttcttcc tttaatctct ttggcttcat
    10201 aatataatat attattaata cagatatttt tcaaaagata tcagctctcc cctctctgca
    10261 taaggagcgc acactgtaac agtaccgcta ccgtcctggg taatataata tcccgtccca
    10321 tttgtagatt tgtttgtagg atcggaaggt atagaagcta caatttcctc tccttcatct
    10381 ttactattca aaacagaaag atctaccata cctacacaat cataaggccc catcttgcat
    10441 atttcatttc ctaactttac acattcctcc ccaatgggaa taacatcagg gatttctcca
    10501 gtcttttttg tatatgacgc aacagcagag agaattaatg acacatctgc atttcttaca
    10561 acatttctat tctcggcgcc ctctttttgg gggttaatta ttagatatcc taggaatgca
    10621 ataacaaaca aagcacataa gataacgaga aagtctagca cagagaagcc ttttttaact
    10681 tcttcttctt cttcttcttt tggaagaaaa tttaaacctg aatttggggc attcataaat
    10741 gtatgatact aaagtaaaca aagaatatct atttatctaa ttctcttttt acaacaaagt
    10801 aatatacaac agatattatt aagctatatc caagaaaaag accaactgcc aaaagtacgg
    10861 tccaaagagt cttgtccttg aagttacttt cttccctcct gatacagtca attagcatcc
    10921 aaattgagaa agctatacta caaagataga gaagtattac taaaatccaa aaccaagcga
    10981 aaccaagagt taatattcca agaaaggctt ccatatatac ataaatatca aattatataa
    11041 aacaagtata acataaatat ccgcataaca cccgccccca tagtccaata atcattttat
    11101 atagtattcc atatctctaa aaatactgat acagaaacaa aaaaatgccc tcccgggcac
    11161 ttgtccacat actaatcatc tctatggata actaatcaac ggagggagag ggattcgaac
    11221 ccccggtccc gcaagcgaga cagagctttt cgagagctcc cctttcgacc actcaggcat
    11281 ccctcctaaa tcttttctaa tgggatatac tccccgtaat ttattttctc taaactaccc
    11341 tgttttctaa tatctttgta taaaagttta tcttgtaata aaacaaagat agcttccttg
    11401 tctttttcgt ctaccaaaaa tattaacata gcaggaaatc tcagactagt ttcaaccaat
    11461 aacatcaact gatcaccttt cttagtaaag tagaaatttt ttaaaagata ccactcgtaa
    11521 agcttcccac ctgtatcaaa accccttgct gttatcttgt gcttcactat ctgtggcttg
    11581 gtagttccca aaacatatat tacaaaaaga agggctatta tagaacccat taggaagtat
    11641 tttcgtaaga tagctaaaag aagtatgaaa aaaagtgaaa gtattacaat tattaagaaa
    11701 ggcttgctac taaatttgaa ttcataccta tctggtgctt cccattcaaa aagcttaata
    11761 ttctgaacaa ctggtttttc atcttcaaag ataggatctt gctctaactt ggtttttcct
    11821 tttactatgt tctttcttct aatattcttc tggttttcta ctgcttcttc ttttgctttt
    11881 ttgatacttt cctctctttt ccttagcccc acttccccgg agattatctg tgaaagttgt
    11941 tctaattcat cttgaaattt gttattttct ttctttacca tacagaaatc atataacaaa
    12001 ggaggctact aacacaacca gaagtataga tattccccaa atccaatcta gtacatgaaa
    12061 gaaattcaaa agaaaaacaa gaagtagccc tagactgaaa aacagagcta cttttgaagc
    12121 tataaatatt ctcatccttc tttttaaatc cttccttagt aatgtcattg tgccgaaaac
    12181 tgccaaagag atagcacaga agacagctat gaaaagaaaa aatagtaata caaagatgtt
    12241 taaccactcg atctctccca ctccattggt agggacaaag tagggattat taaaaagaaa
    12301 aaacaaaaaa ccaacagata cgataattac acttactaaa ctaaaaacac atttcatcgt
    12361 tgttgttctc taggactaaa ataattactt tttagtaaat attattagag tcacttaaat
    12421 taaataactt tcattatgaa tatattaatt atagagaatt ccaatactct tgcccatctt
    12481 cttcaaaaaa ctctggattc ctacggatac aatacatcaa tagactcttc ttccttcata
    12541 aataagaacc tcgttaaagg aaatatcttt gactttgtaa tactaaatat taatctggaa
    12601 aaaggaatct cttatgaaat attggcatat attagaaaat atagtccaaa tactaaaatt
    12661 cttggtgtgt gtaacaaagg tagctgggtt gaaaaggttg aatttctcaa caaaggtggt
    12721 gacgatgtac taacttaccc ttttccgatg caggaggtat tggctagaat tcagtctctt
    12781 attcgaagac cgaagagcta tcttgatgaa agcatatata ttggtagtta tgttcttgat
    12841 acaaagggaa aagccgttct gaaagatgaa aaggaaatta acttgagaaa gaaggaattc
    12901 gaactttttg aatatttggt taggaacaag aacagaaccg tttcaagatg tgagttactt
    12961 gaccatgtat gggattacag acaatatgtt ggatcaaata ctgttgatgt gcatatcaaa
    13021 aggttaagag agaaactcca agataaaaac ctaattggaa cagtacacgg tattgggtat
    13081 caggttaaag acaagaagcc taaagcatca taggcataac tagatgcaca aagttctcgt
    13141 ctcctttaat tttgaatact cctggtctaa ctggttctga gcattcaaat ataatatctt
    13201 ctgaatcaat atggttaaga atatctgcta aaaatttagc actaaaagca attttaaggt
    13261 cctcaccctc tactttacag tcaaatctac tttgattttt ccctaaatca gattgcatag
    13321 cggaaagaga aacaccctct tttgcaatat ctaaaatcat tttgtttcct aaaacagatc
    13381 ttgcaatgat attaataact tttagtgaat taagaaagtc cattctgtta atttcacact
    13441 gtgttttaaa acctgttgga ataatctgct tgtactcagg aaattctcca tctattaatc
    13501 tagaagatag ctctacatct gaaaatttaa aaagtacttg attcctgtct tctattacat
    13561 aaaccttaac ccattcatct tcactgtcta gttcggaaac aatatgcgat atctcctcaa
    13621 atgctcttac tggaattaat aagctcttct gtttaccaga tttctcaatt tttacaatct
    13681 gtctagaaag tcttagccca tctgttgcta caaaggagac actatctcct tcaatttcca
    13741 ttttgatccc cgtaagcaca ggtttaatat catcggtagc tgcagcaaag gccactctat
    13801 ttactgcatt agcaaaatct cccttgttta ttcttacaaa gtaatctttc tcatccttta
    13861 tctctggaat tgaaggaaaa tcgtcaaaag gcattgtatt gaaatttgct gagttgttag
    13921 ttgttgaaat attaaagctt tgtttctcta cttcgaagtc aatcttttct tcagatattg
    13981 aatttacaaa ttcagccaat tgtttcgcag gcacagttat cttcccttca gatttaacat
    14041 ctgagcctat ccatgtgcta acacttaact ctaaatctgt agtcttcatt agtaacttgc
    14101 ctttgactgt ttcaaaagca acattgttaa gaattggtaa accgggttta ttgtttacta
    14161 tcctacttac tatgtttata tatttagaaa acgtatcttg gttacaagaa aactgcatat
    14221 ttatatatgt aaaaagatta agtatgggac agttttgctt ctgtatattg tatcattttt
    14281 tcctcttaag gagaattttc ttgtagcgtt gtaaccctgt tctatatttt atttattaat
    14341 atatttagta gttgtattag tagaggatat ggatatgttg ataactatct ctaatgcttt
    14401 tgcaaaacaa tctttcagtg tgtatttggt atgtggatat ttgaattcaa agatatgctt
    14461 taaggagtaa agattatttg ttttaggtgt gttttctggt gtggattaat ttaggggata
    14521 attttaacaa actcaacaac tatgtggtta tctatatcct taagaaatgt cggtaggaaa
    14581 acagattatt ttgtagaaag gatttcttaa ctgtggaaaa gagacaggtt atccagagaa
    14641 gacagatagt tatccaccga tttgtccaca ctaatctctt atgattttgt agcattcatt
    14701 taatctctcg ttgagctttg ggtctttctt tttgagcaaa tctatcttct cacatgcgtg
    14761 cagtactgta gtatggtcat ttcggtttac ttcttttgct actctttcaa gtggcataga
    14821 aagttcgttt cgaagtatgt acatcacaat ttgtctgcac tgagcgatat atgccgttct
    14881 tctgtccccc ttaatctcag atattttaac atcaaaaaca tctgaaacag cttttatgac
    14941 tttcataggg gtaatctttt ttcttttgct ttctaagtca atttgtagaa gcttggcaat
    15001 atcatcctct gttggattca ttcctaactt aattaacgag agaacctttg tgagagaacc
    15061 ctccaattca cgaacatttt tgtcaatgtt tttggcaatc atctgcataa attcttcagg
    15121 aacttctaca gaactattct tttcaagaag gtactgttta agtattgcca ttcttgtttc
    15181 atagtctgga gcttggatat ccgcaaccat tcctccttgg aagcgagatc tgagtctgtc
    15241 cgtaatgttc ttgatttctt ttggaggtct gtctgaggca agtataattt gtttgtttgc
    15301 ttggtaaagg gtattaaagg tatggaaaag ttcttcttga gttcttggat atgtctctac
    15361 aaactggaca tcatcaatca ttaacaaatc tacagctctg tatttatttc taaaatcttc
    15421 gttccttttt gttctaatgg cttcaaccat ttcattaaga aattgctcta ttgatatata
    15481 tacgactttc ttacttgcat ctctattcaa tacttcgttg cctattgctt gcattagatg
    15541 ggtctttcct accccagtat caccgtagta aaaaactgga ttgtaggcat tccccaaatc
    15601 ctccactact gcttctgcga ttgcctctgc aagtctgttg tttgaaccaa ctatgaagtt
    15661 agagaaaaga tatttaggat taagttgtgc atttttaagg gcgttttctt tttgcttttg
    15721 ttctttttct acagaagaaa aaagatccaa tgaaatatcc tctttttgtg aagggtcgtg
    15781 atacaggtat ttctctttcg gaaaagtgtc tttttctctg aggttaatct ctatctcaaa
    15841 atttcttcca gatgatttca agagagcctt tttcaaagta gctttgtaat ctcttagaat
    15901 cttctctcgt ttatatgatg catcgcatga tatttgtacc aagccatttt gaatattctc
    15961 tatgtaacaa ccaccgaaaa tacctccgaa gacttttgga ccaataatca atcgtaggtt
    16021 ttctttagca cttttccaaa gttcatcggg atctgtttgt gtcttttgcg catgtgaatt
    16081 gagaatctct ggttttggta atttttctac atgctctgca aagtctgctt tatccattat
    16141 aaagatgttt aaaatattta ggcagttttg agggcagtgt ccgatcgatt gggacttttt
    16201 ccattatatt gaatttcaaa acaatgtgga tatctttatt tgagcttcat agaaatgtag
    16261 ctaaggcaag ggtttgtatt tctcttaaag ggctctaaaa ttactagagt atattcaggt
    16321 ctctgtgcat ttgccaaaga ggtatgctta ctatataatt accccgacat taaatattga
    16381 taacaacaaa tgaaaagaac atatcagcca aaaaacttaa aaagaatcag aaaattcggc
    16441 ttcatggaaa gaaacagtac taaagctgga aaagatgttt tgaaaagaag aagagcgaaa
    16501 ggaagagtaa aactttctgc aagcgaagag tatagcctct tgagaaaaaa gccaacacaa
    16561 agtataagat agtaatatgc ttaataaaaa atacaggtta tctgtaaacc aatttccagt
    16621 tgtgtataaa aatggtaaga aaattcgtgg taaatacggg atgttaattg cgttaaaggt
    16681 agagaacagt tctaccccac aatttgggtt tgtagtaagt aaaaaaattg gtggtgccgt
    16741 tcaaaggcat agatttacaa gaattctgag agtagcagta gcagagagta taaaggaaat
    16801 ggagttagat aattgtggtt atatttttca gtatgttgct tttgaatttt gtgacagtaa
    16861 agatgttttg aaaaaggaat tacaaaatca gttaaaaggg atattaaatg attaaaaaag
    16921 gagttttatt tttgataaag atatatcagg ctctgttttc tttagaccat ggtcttttag
    16981 gaatgctgtt tccaaatact aggtactgta agtttatccc tacttgttca gagtatggtt
    17041 atgaggcagt agaaaaatat ggggttataa aggggagtat tttagctata aaaagagttt
    17101 taaggtgtaa tccttggact aaggagattg ggccagatcc agtcccttaa ttacctattc
    17161 gcaaatatat gaatatttgt atataatcgg aagttacttt agccaactgg aaaaatgttt
    17221 acaacaattt ggaacacagt actttattac ccgatcctaa accttatggt ttctttctac
    17281 catttatttg gggataacct tggttgggca attatagcga tagcaattat tctaagatta
    17341 atccttattc cttttacaaa aagtcaggca aacatgacaa agaagatgtc atctttgaag
    17401 cctcagcttg aagaattaca aaagaagtat gcaaataaca aagaaaagct ttctcaagag
    17461 caggttaaac tttacaaaaa agttggttac aaccctttgg gttgtctagt gagctttatc
    17521 cctcagttaa taattctttc tgttttgatt gcagttttaa gagctgttac aaataataca
    17581 tttgaaggat tatatccatt tgtaaaggag tgggcatttg gtgcgagtga tgtattcatc
    17641 agtactaagt tccttgtttg ggatttggca aggcaattaa atggaaatgt tgacggggag
    17701 cagttaaacc agatttcaga atttatgaaa tatagtccat tgcagattat agagttattt
    17761 aaagatggaa caatctcgag agagactatg gccataattt ccaaatatgg catctcaaat
    17821 caatatggtt taacatccat acattctatt tattacatga tacttatagc tcttgttggt
    17881 atctctcaat ttttcaccag caaatttaca caagcgattc agaatccaaa tgggattact
    17941 gctccaactc cacagaaaag taaaaaagga gatgtcccat caatggagag tatgcaaaag
    18001 cagatgacta aatctatgaa cttcttactt cccgcaatga cagtcttttt cgcaatctcc
    18061 tatcctgcgg ctttaagttt ttactggaca gttcaatctg ttatgttaat agtacagtat
    18121 ttcatcttag attttgacaa aacaaaggta ggtgtccaaa atctttttac aaagatggtt
    18181 gataaagcaa agaagagaag atagaatagc cccactctat tttgaaatta aattattata
    18241 tttatcttat gaaagatgaa gatattgtta aattagtaaa taaagaagta gataaactct
    18301 gtaagcttct tgaggtagat tgtgagagag attgtcaaat agaagatagt gatactggag
    18361 ttaaatatat taagatatct tttgagggtc aggatttagg atatatgatt ggtagtaggg
    18421 gaagacatat ggactctttg caattcattt tacaagtgat aattgggaga caattggacg
    18481 agggaacaga tttcagagtt tttgtagatg ttggtgatta cagaaaagca aaagatgaga
    18541 gccttgaaca aatggcatta cagaaagcag atgatgctag acttctcgga gaagagatag
    18601 aacttccgcc tatgaagcct tctgacagaa gggttatcca tttaacattg gacaagttca
    18661 aagatattaa aacagagagc agaggagagg acagagatag gcatattgtt atcattccta
    18721 taaaagaata gcgattgttg gtacaatcga gtaatgaaga agatttcaaa agattatatt
    18781 tttcttggca ttgtaatcct ttttagtatt ttggtatcac tactcttttg tagtgagaat
    18841 cccctgcgat taattacaat tctttcttct tttgttgtac ttttctgttt ctattttttc
    18901 agcaaaaaca tatttttctc atcttttctg tatatattaa ttaccctacc ctttaacata
    18961 accttccaga ttccacagag tgttcaaata tttggtaacg acatattact ttataatcca
    19021 tatgtagagg gagtgtatgt taactatctt gtccctactc tttcaattct tgatttaggg
    19081 gttatcctgc tcttattagc tcctttacta cataatggat ggggaaggtt tgtagatata
    19141 ttcaaaagat atgggattgt tctgcctttg tttgttgttt tccttttgct tcagaatgtt
    19201 ctactaaatg accctctgtc tttcttaaac tctgccagat taattctcta tatattgggt
    19261 tttctttttc ttcttgaatt ctttaaagaa ggaaagaaga aatattcaga accgttgctt
    19321 gtggtttctg taatatctgt tttaatacaa ggaattattg ggatattgca atttacaaga
    19381 ggaagttctc ttgggcttgg tttctttgga gaatctcagg tagtaagcgg catgcaaggt
    19441 tctagctttg taacattagg tggggaggtg tttttgagag catatggaac ttttcctcat
    19501 cctaatcttt tggctgggta tttgttattc aatatattac tcgctcttac atttattaat
    19561 catgagaaag ggaggtttaa aattcttcca tatattttgc ttcttttatc tttaacttct
    19621 ctactcttta cattctcaag ggtatccata ttgctagcag ttctagtcct aattgtctat
    19681 tttggagcaa agattgttaa tggtaagttg tttgcctttt cgttacccct gttctttgag
    19741 agatttgtta atcttttctc ttctggggat acaagttggg aggacagagt aaagctagcg
    19801 agatctagcc taagggttat taaggaaaat tggttgtcgg gaattggcat tggtaatttt
    19861 gtaaaaggaa tagagggaga tgttccaagg agtgtaaacg atgttttgct tcttcaacca
    19921 gtacacaatt tttttttatt aactttctct gaattgggat taattggttt tggattgtat
    19981 atgtggttgt tggtagatgt atgtaggaaa aacattgtaa agattacact gtttggtgtg
    20041 ttggtattgg tgtgtatttt tgttttgata tcttttgatc actacacctt ttctcttgca
    20101 caggggatta tgcttttctt tcttttgctt ttgttgcttt tctaacatct cttatgggtt
    20161 tgttttttgt tatggagtgt ttgttcatat gtttggttct ggcggagaga atgggattcg
    20221 aacccatggt atgatttctc atacgcacgc tctccaagcg tgtcctttcg accactcagg
    20281 catctctcct tatggcgtac ctggccggac tcgaaccgac aacctactgc ttagaaggca
    20341 attgctctat ccgattgagc tacaggtaca tataacaacg caattctatc aggaaataat
    20401 ggcaaaataa aggctttaca tattgtatat ctcaacaatt atgcctcctt gtctctttgg
    20461 aaagtaagga tatttagaaa ggatattctt tgggaagaaa ctagcagata ttcttttctc
    20521 agagaaatgt aatgtttgaa gatattctaa cccctgctcc cacttcatct ccttaagggt
    20581 attaactatc tcttccttgt ttaccttagg actgacatct ccttttgcta caagctttat
    20641 ctttacacct tttttactat cctctactac agaaatagtc ttctctatac tattgcttaa
    20701 accaagttcc aagtcattat caccttcaaa cagatttttt tcctgagcct ctttcgttag
    20761 caaagcggaa attccgtcat caaagccctc tgtcagatag taagttgcag agcttttaat
    20821 ctttaacgaa aggtcaacat cactacctgc agctccaact gcaacactag tttttatact
    20881 atcttttaca acttcactct tgattgaatc ttcgattatg ctccactcgt ttgaagcatc
    20941 tcttagctcg ttttcagctt ctagttcagc ttcacttttt agtttttcta ttcctgagtt
    21001 cacatcactt tgagaaagga cagtgtattc ctcctttgtt cctcctgtga aagcactggg
    21061 gttatatcca aaaagctgtc gatccaagtg accttgtgct aaaaagtttg agttactttc
    21121 gatattgaat tctgttccga aatctctcgc atgaacgctt acttctacta ttcctggaca
    21181 agggactgta atggcggtat ccatagtaaa agctttgtcg gaggacaaaa ggatttgtcc
    21241 tgcaggtaat gcgattgcct ctgtgcatga aggacctccg accaattgta ctttgccagt
    21301 tgccttttcc ccagtgtatg ccttcccagt tgcttttatt gtagaagatc tgtctttttc
    21361 tactgactca acctttattg gaatttttaa gttgtcaaaa tctatctgtt taatattttc
    21421 atctcctaca aagattttct ctatgcttac ctctttggat tcaacatata ctgcaacctt
    21481 aacaaaagga acagtctgta tgtatatgat tgcggcgagg attgtgacta agataaggga
    21541 gaggataccg attggaagat attttttaat accagtaggc gtagatattc cagtaccttt
    21601 cttgaatggc caaaaagctt tgaagtttac agttggtaat ttgaaacctt tctttaagag
    21661 cggttttgga gtgatagtct cttctgggaa atcttcaaac ttaatgggga catcttctat
    21721 attttctggt gcttctgttg gtaaatcttc atctaaagca agaaataccc cctctttctg
    21781 aacatttttc tcaactgctt ttgtagcaat tccttcccta cttctttgta ttgcatcgtt
    21841 tatttttttc tcaaatgtag atgcactttt ctcttcgggt agttcctctt ttttctcgag
    21901 aggcttatct tttggctttg gagcaagtct agctattttc ttctcctcag cttctatcca
    21961 catttcctct gtaggattat tgggagaatc tgttgtggtt aatccggcaa tggaggaatt
    22021 tcttagacca gttggatttt taattatttg tgctacaaga actttttctg cctcatctgc
    22081 ggtctcttgg agaacagaaa ggttgattgg acttatgagt agatcacttt cgtctgcgaa
    22141 ggtaagtatg actctttcag tttcagcctc aagaattcca gtaactacat ctgttaattc
    22201 gtcatctctt ccaattacta attttgtatt tttctttatt tcatccatta tatctttata
    22261 ttcctattct tacttatttc taaatcatat gcaaacttcg taagggaagc aggggtaatg
    22321 tcgaaaacat gcttcaattc cccactatta tctactattt tgtccaatct atctggtttg
    22381 aaaaattcaa ttcttgggac aacggtaaat ggtaaatatt ttttccatgg gaactctagc
    22441 atagctccct tcatttctgg taaaagagca ccaccaccac atagatatat ctgtggaggg
    22501 aaggaatcta tatcatcaca agaggcaaga gctgttttta atgtcttcat ccaaagtttt
    22561 gaaacagggt atacaatttt ttgaattgta tttgaaagtt ctcttgttaa ttctttttct
    22621 gcatatttaa tttttctctg ttcagcatgt cttatgtcta tttgaaggac tttagcaatt
    22681 tcttttgtga aggttcttcc tccgaaggca aacatcttgg tctctactac attacccttg
    22741 tcaactacag ctatatctgt tgttccacca ccgatatcta tgaagatggc agaaaaatct
    22801 ctattagatg aaccgctaaa tgctcttgct acagaaaatg gttgcgagac aataccaatt
    22861 acttctaagc caagttcctg agcaaccttt cttagtgcct cggtgtatgt ttttggggca
    22921 aaggatgcgt agaaatgtaa ttttacttct ctacccttgt atccaatcaa ggaatctact
    22981 ggcatccccc ctatttccaa tccggtatgg gtaatgtgta gtatgtctat atcttcattt
    23041 cgtaaaccag tcctttgtcc aagatcttct tttccgtttg tttcaatctt ggcttttatc
    23101 tgtccaatta ttttgttttg ttctttctgt gtcacctctc tatcgtagtt ttcctctctt
    23161 tggtaattta cgatgataga gactccctgt atatattctc cagcaattcc cattactaca
    23221 ttttttggta attcttcatc tgctaatctt gaggttaatt cagagataga aagacgacaa
    23281 ttatctaaga cagtatctag attagtaatg atacctttgc tcattgcgtt ttgttgctgg
    23341 tcgattctag atatcttttt tatatctatt ccaatatctc ccatcgagaa gaggatactt
    23401 ttcaattgct ctgtacctat gtccagcgca attaaattgg tagtaccatc gagaagattt
    23461 tttgatgaat tttttaagaa aggcagtcct agcatatatg aaagtataac atatttgcag
    23521 gtatttaaat gatagctgtt agaataattg gttctctctc agttcgtttg tatatgagct
    23581 ttgaaagttc tttctctatt ctctctcgga gctcttcatc tttaaatact ttttctcttt
    23641 gtgatttgtt tagccaatct ctatgaatat ccattacaag gttctctatt tcttgaagaa
    23701 gctctcttga gcctttcata tatatgaatc ctctagagat gaatcttggt cttccaatta
    23761 tttttttgtt cttggagctg atattcaaaa ttacagcaaa gattccatat tcagaaagct
    23821 gttttctgtc tttaagtaca atatctccga tatctcctac tccaagtcca tcaatgagga
    23881 ttggcttaga ttctatcttt ttacctctac tccatgagta accattgaaa agccatgtct
    23941 gtccatcatc tgtaagaaaa atgttctttt catctactcc ccatcttacg aaattctgct
    24001 tgttgaaata tctgaatgta agaggaccat gtattggcat tatatattta ggttgtatga
    24061 ggttgtacat catcttcata tcttcttgat ttccatgacc agtagaatga atctttaact
    24121 caacactatc tttaattaaa tctgcaccta agccaattaa tctatctgtc atcctttcta
    24181 tactactaat attctctgga atttcagaag aagacattat gacgaagtct ccctttttga
    24241 tctttatgaa cttatgctca ttgagagata ttctgtttaa agcagcaaat cgttctgctt
    24301 gacttcctgt acaaagcaaa agaagttcat tgtctggata ttttgacata ttcctctctt
    24361 taacaatgag attatcatct atttttatgt aattcttttg tcttgcaata tctatagatt
    24421 gttctaaact cttccctgag agaactatct ttctgtttgt tcttttggca acctctacaa
    24481 gagagtaaag acgagtaatt aaagaggaaa aagctgccac aattactctt cccttctgtt
    24541 gggagattat ctctcctaag ttttggaata tttcagtctc agagggtgct ttgccagggt
    24601 tagatgcatt agtactttcc attaaagcta agtctatttt ccctcttaat tgtctcaatt
    24661 tctcataatc tgtttgtggt tcatttgcag gagacatatc gattttgtaa tcaccagaaa
    24721 agaacatatt tcctcttgga gattctaaaa agattgaaaa ggcatctgga atactgtgat
    24781 taatcccaat aaaggtagct ttgaagtttc ttccaatatt tatagtagat tttctgtcta
    24841 cactaataat tttgaccttg ttttccattt tgtactcctt catctttact tcaatgaggg
    24901 ctttggcaaa tcctccagca tatacaggag ggtatccaag ttctggaagt atccacttca
    24961 gtgcaccagt atgatcaaga tgaccgtggg ttatgagtat ggccttaact ttttctttat
    25021 tcttttttag gtaactaaca ttgggaatta aataatcgat tccgtacatc tcgtgtcctg
    25081 ggaatgcata tccggcatct atcatcacaa tttcatcacc atattctaca aaattacaat
    25141 ttcttccgat ttctgctgtt ccagaaagtg tacataattt gagaaattct ttactatttt
    25201 ctttcatata gcccattata tcagatattt ggatgtgata taatgggaaa gcctttaata
    25261 caatattagt aagagtaata acatgttaag agatattcca aaaacgtttg aatctcagga
    25321 tatagaagga agaatctttc aaatgtggga agagagcaag ctagctaatc ccgaagtagt
    25381 agagaagtat tttcaagaaa aaggcagaga tattaaagaa cctttcacaa tcattcttcc
    25441 tccccctaat gcaaatggta accttcattt aggtcatatg tgtggatatt cataccatga
    25501 tgtgttggca agatatatga ggatgacaaa ccatcctact cttcttgttc caggtaaaga
    25561 tcatgctggt atacaaacag aaacagcatt tacaaaaatt ctagagtcaa aaggagaaag
    25621 taagtgggat attggtagag atgaattcta caaaagatgt tatgatttct gtatagagaa
    25681 tgcagataac gccagaagac aagaaaaaag aattggtctt tcagcagatt acagcaggga
    25741 attttttaca ttagatccta aattaacaaa aattgtttac gacactttct acaagatgta
    25801 tgaagagggg cttgtttaca gagacaagag aattgtaaac caatgtccta attgtaaaac
    25861 agcactagcg gatatagata ctgaacatga agaaagaaga ggaattttcg catatattag
    25921 atatccattt gtagttggaa acgggaaaga tgggataact gttgctacta ctcgtccaga
    25981 aacgatgctt ggagatactg ccgttgcagt taatcctaaa gatgaaagat acagtaaata
    26041 tattggtaaa aaacttcttt tgccaattgc aagtagagaa attccggtta ttggtgatga
    26101 ggaagtagat atggagctag gcacaggagc tgtaaaggtt acccctgctc actcccctgt
    26161 tgactttgaa attggtaaga aacacggact ggaaattgaa aatgtaatag attccgaagg
    26221 aaagatgact ggaaatatac cagagagatt taagggaatg acaacagtag aatgttcaaa
    26281 agctctttgt gaagagttag aagatttagg attactagag aaaattgaaa atattaaaca
    26341 cgaagtatca atatgtgaga gatgtaagac tccaatagag ccaatcatct caaatcagtg
    26401 gtatctaaat gtagaaacct tagcacagaa agctctaggt gcgatcaaat ccgaggaaat
    26461 taaagtgata ccttttgggc aacagaaggc attggaattt ttctttgaaa atattcaacc
    26521 ttggtgtata tctcgacagt tatggtgggg acagagaatt cctgtttggt ataatggggg
    26581 aaaagaactg tatgactggt taaatgaaaa taaaggaaaa agtgtagcag actatgaaaa
    26641 tgaaacagga aagaaagcga atggaactgg tgagattaag ctaggagaag ataatcctgg
    26701 agatggttgg gaagcagaaa cagatgtctt tgacacatgg tttagttcag gacaattgcc
    26761 ttactctact ctaggaggac cagatggaga tgattacaag aaatactacc ctacacagat
    26821 aatgattcat gcaagagata tcattttttg gtggtcagca cgaatgataa tgatgggatt
    26881 gtacagaact aaccaagtac catttcctac tgtcttttta actggcatga tacttgcagc
    26941 agatggaagt aagatgtcaa aatctaaggg taatggtata gagccaagtg aagtttttga
    27001 aaaatatggg gcagattctc ttcgactttg gtattactcg gatgctcttc caggttcaaa
    27061 tgcaccaatt agagaagaga agattaaagg gaacagaaat tttgttaaca agatatggaa
    27121 tgccagtaga ttcatattaa tgaatattga tgaaagtgag atagagggta ttaagaatca
    27181 tgagccattg gatacagaaa gaattagcag aacaagagag cacattctta gtgtttccag
    27241 atatattgag aagtaccagt ttaatcttgg agcagaagcg ataagggaat tcttctggca
    27301 tcagttatgt gatgtatgga ttgaggaaat taaagaagag attaaagatc agccgattgg
    27361 tagtgatatt agaattgaga agctttccga attactttgg atattgaaaa acaatcttaa
    27421 gattatgcat cctttcgtcc cgtttgttac tgaagctgta tggcaagagt tagtaaagat
    27481 tggtctagca gaggatttgt taatgggaga agaaataaaa taacagggaa aactggtgtg
    27541 atgaaaacca taatggttct ccctgttatt tgagttcgga tcacctcctt tcagtactta
    27601 gacttccagg attattcaca tccgactcct ttctttagtg gaatcttttt ccgttcgttc
    27661 tcccacaagg tttctgtttc ttcctttccg acactttttt cttttttttt gtcacatatt
    27721 cctccgatgt atggattggg aaacagtccc attaacaatt gtaaagaaaa atcctcgtta
    27781 tgtccatgat gtctagagaa gagaaatgaa atgtaatata atcaaaagta ttatgaacaa
    27841 acaaaaaata tatattacaa ctacacttcc ctatgttaat gcagaaccgc acattggtca
    27901 tgccttagaa tttgtacaag cagatgcact ctcaagatat tttaaaaaca aattaggaaa
    27961 agaaaatgtt ttttttaatg taggtacaga tgaacatgga cagaaaatat ttaacaaagc
    28021 aaaagaggaa ggattatctc ttaacgattt tgtagacaaa tatgcacaaa gatttaaaga
    28081 cttttgtaaa ctattctttg ttgaatatga taacttctac agaacctcta ctccagaaca
    28141 ccatgatgcg gcaaaaacct tttggaaaaa atgtgaagag aaaggagata tatacaagaa
    28201 gagatactct ggaagatatt gtgtcggatg tgaaagatac ataacagaaa aagaattggt
    28261 ggatggcaaa tgcccagatc acaagacaat tccggaatta aaagaagaag aaaattactt
    28321 ttttaaactt tctacataca gagaacctct tttgaaatgg ttagatgaaa acaaagattt
    28381 ccttaaacca gagtctaaaa ttcctgaatt gagaaaaata attgcagaga tagaagatat
    28441 tagtatttca agacttaaag agaatcttcc atggggaatt gaagttccag atgatccaga
    28501 acaagttctc tatgtctggt tcgatgctct cactaattat gtaaatgcag ttggttttgg
    28561 aacggatgaa aaaaaattga atgaatggtg gcctgcaata cagctatgtg gtcctgacaa
    28621 tttgagattt cagggtgcta tatggcaagg aatgcttgct tctgttggac tcccccactc
    28681 ttctaagttg cttgtacatg gaatggtttt aggtccagat ggaacaaaga tgtctaaaac
    28741 cattggaaat attatctctc cttttgacca agaggaaaaa tttggagctg aaattgtaag
    28801 gttctatcta cttacgggca taacgacata tggagattgt gcatacaaag aggatgacct
    28861 tattaatatg tataactcaa gattagcaaa taatttcggt aatcttttaa acagaacaat
    28921 ccatcttgca aacaaaaccg cagttaatat caacaatgag caattagtag aggaagattt
    28981 caaaaagact gtagatacat atgtggaaga agtccgaaga ttgtatgagg attttgaaat
    29041 atctcttgct ggagaaaaaa tagatgagtt agcagatttt gggaataaat atattacaaa
    29101 agaagaacct tggactaagg aaaaagaaca aacagaaaaa attcttaaca accttagcta
    29161 tcttttagag aaagttatac atctttacaa cccaataatt ccacaaagtg cttccagtgc
    29221 aatacagcat cttattgcta gagaaacagt aatacttttc cctaaaatag agagcagata
    29281 atacatttat atgtgattat tatgctatac taatttagaa aagattttaa aactgccaaa
    29341 tgtttaaatc agaaaaacaa ccaaaatcta ttgattttct tcagcctgta tattctccta
    29401 ctgacatatg gtcaaatgca tatatatggt taacagaagt tggaaaatat cttttaattg
    29461 ccgttgaggt tgtggttctg ggagtatttc tctctagatt tattttagat aagagaaaca
    29521 atgatttaac cgaggaggta aatagtaagg ttactctact ctctaatgac acatggaagg
    29581 gcaacgcaat gctttttgac aactaccaat ctctgctttt ggatgttaag aaggttcgtg
    29641 aagaacagga gattaactcc accactataa gtgaattaat aaacggagta ccttcttctt
    29701 tgaagattaa gaacttttcc ttctctcgag ggaaagttgc ttttcttttt acagccaata
    29761 atcttgatgc cataaagaac tacgaagctg cactcaaaaa caatacagat taccatgatg
    29821 ttagattttc aataaccaaa gactcaaacg aaataaatgt tcgagttact ttctctttgt
    29881 atgaggaaaa agaataatgg atgaaaagaa tataaataca acagaatata taaagataat
    29941 taaagaatct cctgagaaga agaagtcata tatctttgtc gcagtaaccg tagctgtagc
    30001 aatcgtcctt gtaatatttg ctatccgacc aacgattcta acaattacta gaattaacaa
    30061 agagattaaa gataagcaac ggatagatga agtgttaacg aaaaagattg agactttaac
    30121 agagctggat aaacagtata cacaggttaa agatagtttc tctggtttag cccttatata
    30181 tcctgcagat gcgaacttca gtttgttatt agcaaatatt gaatccgtag tttcaagaaa
    30241 tggttactct cttggtagtg tgggctttga caaatacgac aagaatgata attataaaat
    30301 aaatgcaaaa tatctcgtac cttggtcttt gagagtcaca gtaaagggaa gggttgggaa
    30361 tatagtaaac cttctaaaag attttgagag tatgccaatg tatccagttg ttgagtcact
    30421 ctcctattct agccaagtag ataaagatgg tttaaccact tttggtatca atatgaggat
    30481 atatcatata gaaaatagta atttttacga ataaaaatat ggaaatgtta aaaaaagcaa
    30541 taattgctct aatcctacta ggggtagttg ttcttgcctg ggttggtggt tctatttact
    30601 accaaaatac caatatagag gttagtccaa acgcacagga atatacaaaa actttgagag
    30661 attcttttga tttagaggaa ttagaaaaga taaccgaaag gacagagaat tcttttccag
    30721 tatctccttc cgaatttact gttttaatac aagggaatta atcgatgtag tcgtctaact
    30781 tctgttgtgc agatttctct tttaatttct ttaatgcttt tgcctctatt tgtctaatcc
    30841 tttctcttgt tactccgaat tcttttccta cttcttctaa tgttcgtgat actccatcat
    30901 caaggccaaa tctgagtgaa aggactcttc tctctctatc ttggagtgta tctaatatat
    30961 ctttaagttg gttttttaga tattcagctg tagcaaaatc ttctggagac tgagaccaat
    31021 catcagggat aatatcttga agcttactat ccttttcttc cccaattggt gtagagagag
    31081 gtgcagggtt ttgtttaatt tttctaattt ctgcgacctt actaagttca atatccattt
    31141 cttttgcaat ttcttcatct aatggaggtc ttccaagttt tgtggttaag acattgttca
    31201 ctttgttgaa tttgttgatt gtttcaatca tgtgtactgg tacacgaatt gttcttgcct
    31261 gatctgcaat tgctctggtt atggcctgtc taatccacca agtagcatat gtcgagaatt
    31321 tgaaaccttt tttgtagtca aacttctcta cagctctcat taaaccgata tttccctcct
    31381 gtattaagtc aagcaactct aatccactct tactgtattt ctttgcaata ctaaccacaa
    31441 gtcttaagtt tgcagtaatg agcaattgac ctgcttcttt gtctccgttt tcaattcttt
    31501 ttgcaagaat cacctcctct tcaccggtta agagagggat tcttccaatt tcgtagagat
    31561 atgacctgat tgcatctgta gaaaggctgg actggatagt cttaagtatt cgaatcttct
    31621 tttcgagggt caattcctct tttacaacct cttccaaagt atcttccggc tcaagaacct
    31681 ctatctcatt ctcctgaagg gatttgaaaa tatcgtctag caattcaaca tcttcctcga
    31741 tagtaggaaa gatatctaat agatcttctt gggtaagaaa tccttggtct ttacccttgt
    31801 ctaccaagtt tttgattgct ttacttttat cagtcatatt ttaattatgg tataatttat
    31861 atatggacag caaaaaagac gatttaaatg acaaaaaaca gttttttata gatacaattg
    31921 caagattttg cgataagtgt gggtctccat ttgtaataga agatgttaat atactccaga
    31981 gaaccggaat atccagcatt atacactttt cgtgccataa ttgtaaatcg aaacatattg
    32041 ctacattcat ttctccaatt ggaatgagtc atagagttcc tgtaaataca gatttgtctg
    32101 taaaagaaat tagcaaattt gcccaacaga gcgagacttc tactcaagag attctggata
    32161 tatatatgta cctaaagaaa aattcgaaag ttttaatcta gtttgtttct ctccatacga
    32221 tatcaaaact tgtatctttg tttaattcga atctgctaga tagttgattc tgaatagcat
    32281 ttaaattgcc tgcattctct atattccacg aagatggata gatgatatct aatttgtatg
    32341 catgagattt ctctcctggt tgtttaaaaa gatcaatcgc taggaatata ttgtcaccat
    32401 ttttaacaat tggaaaagag gcattctctg gagtcctctt taattggtat gtaatttcca
    32461 aggtattaac atctttaata tttgtattaa accatccccc caatacttta aatccactct
    32521 ctttgtatgt cgtgtattta ttgtcgtcca ttcccttgac agaaataatc gtagcatatg
    32581 aagggacata cactctttgg taattaatat agttgccttg tggatataca agattttcag
    32641 aagtattttc tgtagcaatt gtatatttaa aaactatggt atcctcatct ttaatatcta
    32701 cctgtaagtt gtaattcttt tctagataaa gatttgcctt gtttgcaccc cagttccaat
    32761 caattgcaat tggagcttcg ttgtattttg aatccagcga atcggcccaa gctctagagt
    32821 taaagaaatt gaaggcatct ctgttcttaa atgatgcttg caaatgtttt tcatcaagag
    32881 actctccaaa aacatcggaa agagatatta aatctgagat gttttttgaa aggagtttct
    32941 gtattatttc atttgctagg tttgcaagga aagtagtctt ttgagttgtt cctggtgtaa
    33001 aattctcatg taatttgaat atcttgtcat aaagattatc gcttgttaca agttctgttt
    33061 ctcctggtac ttctattcca ccccatttac ttagtaattt ttgtataaat gttatatcta
    33121 tagttataat cccatccaga tcgtctcctt tgccaagtgc tctaatgaat ggctctgcat
    33181 ttaagtaagt ttctgtcaaa tctggatacc agttaacaag agagaagttc caagttgtca
    33241 atccaagtgc ttgattcatg gatttaggtg cgttgaatgt cttaccttgc actttcaatg
    33301 tcccgtcggc attgtaaatg tcatcgacaa aaagttccct tacctgtcca ctttctatac
    33361 ccactacacc gtagctagtt aaccatcctc cagtgcttcg aatctcagat tcgttttgta
    33421 gaagtatgag atatctttgt cgaccatcca ctcctagcaa atcaggaagg aatgtaatta
    33481 tttctttgta atcagatgtt gtcgaatcta atttctctac caaatctttc aattctagaa
    33541 ttttgtcttg tagaaatgtg ggaaagacag aggtattaac actgttcacg atgtcatttg
    33601 aaagagagat tttgtaagta gcatcatcta tcttgtactg gttttctttt attgctatta
    33661 gatattctct gtactctctc gaagtagagg gcagtgaacc ttggatatct actgcaggtt
    33721 ggaaatcttt aatgtaggat gctaatggct cgatggaatc taaaagaccg gatgctccat
    33781 ctactgcgta ctgtgcacca actattaatt cgacggaatt gttgtaaagg tctgttcctc
    33841 cagttatttg gaacacccat tttaagttac tcaatgtccc tgacactcta tcaatatttt
    33901 tatccaggac agtgatctct ttttttgctt tggaagtttc taaatcaagg agagagttgc
    33961 taagagtttt ggctgatgaa gaaataatcg ttagtccaat tagcgtaccg ataacaggat
    34021 agacaaaaaa gtaagtaaag aggactgtta gaatagatat cccaacatat ttagatattt
    34081 gtgaactgtc aaaatctttt tttgcgaata tgttttttat tggtgtgaaa agcttcgata
    34141 caaagtttcc aagagaagtt ttgtatattt ttacattgga tttttctttt ttctctaatc
    34201 tttgcttacc ctcaacatcc cacttcttgt ttatactgtc atagtaggtt tgaatttgtt
    34261 caattagaga gtcttctaga gttacctgct gtatccagcc atattttgta gcatgaggag
    34321 ctggtacata gaggtcttgt attacaaatt cagtattttc actttctgtg aacttaatgg
    34381 attgtgcttc tgtgttcaat tcgagtaatt tgtaagcgat agagagggtt gtgtaattat
    34441 ttttgtttgc taaggtaatt acctctcctc ttgttttgtc agaaaaggtc agtttaagaa
    34501 ttccatatgt agcatcgttt tcatttatta cattgtgtaa atctagacct tctcctttta
    34561 ttgtaatctg tggtttttct gtagcatctt tgagtaggtt atgtagggtg tcgttgtcta
    34621 cacggttaat tcctttgcca agaaggtttc caattcgcag tatcctaata ttcaaatttg
    34681 ttttgtcttt aaactcagct gctaaggtct cacagtattt ctgtaactca atgttggagt
    34741 atggagaggg agatgctact tttgaattgt tcaccctgtt agaaagttct ctgttgaggg
    34801 caagggaagt tattaaggag aatttagcat tgttttttct tgcatttctt agtgttaaat
    34861 ctaggtatcc tgtttctcga agaaattctt tactgtcaaa atctttttgt gagagttgct
    34921 cattaagaag atagaagagg tagtcaaaac gttttaaatt tttgaaaaga gcttccatgc
    34981 ctttgaattc aaagaaatcc acactcttag atttctttaa ttctgttata tattcttttg
    35041 tttgggcatt aaacttatca ataataatga catgacttcc ttgctctatg agggttttgg
    35101 ttattaaata acccagtctt gaacctccat gtacaatgac tgtcactggt gtttgactct
    35161 tgatttttgt tagggcagaa ttactcattt gtatcttcta cattatcaaa attaacgggg
    35221 atagggaaat taaaaacttt tagatttagt aaatagcctt ttctaattat aactggaact
    35281 tcaacatcca tataacccag tgctataacc tttatttgat attggttttc ttttaattta
    35341 acctctgtaa ttggtaaatc atttaaatat tcaccatcga tataaagttt tgctttttga
    35401 gcattgcttg taaccgtaag aaagccctct gaagcagaga tctttggttc tggtgagtaa
    35461 tacattatac ttccatgaag gatattttct gggccaatct ccaaatctat tctagatgat
    35521 gtgtttttgt aaagtggaat tttaaaggtt tgttttttgt aaaaagtttc cccagaagct
    35581 atcttttcaa gttcaattgt gtactctccc tctctaagat tctctatttc ctgtggagtt
    35641 tctccatact cttttccatc tatccatatt ttagctttac cattttgcaa aactatttct
    35701 agggtagtgt tgttgtaaaa gcgtttaatg actggaagag agccaattat tctgttaatt
    35761 ggaatgaagt agaaaaggat agaaagcaag agtatgcttc ctactattat gatggcagtt
    35821 ttatggtttc tcatcagtta aattatatgg aagagtaccc tgtttgtcca ctaaagatta
    35881 atatgaatac ttggattcag gagatttctc atcttcattt ccaattcttt ttcttgatgc
    35941 cttgatattt atgaatacta caaaagcaat tccaagcgaa acacctagta ttaaagcaaa
    36001 atatcggata gcaccagtgg ttaaaattgc aaaggaacca agaagaagag tagcagacat
    36061 ttcaagaagg acaacctgcc ttcttgagag attaagtttt aataattgat gatgaagatg
    36121 gtttgtatcg ttaattctaa gcaatgttaa gagatttttt ggttttacta caaaaattct
    36181 ttttacaatg acatatatga aatctattaa agggagagta agtaacatga gtgttgttga
    36241 catcttactg tcggcgataa ttgcaaggat gcagatcaaa aaaccataga cagttttccc
    36301 tgaggagcca ctcataatct tttgaggtgg aaacgcgaac attacaagga caagcaatat
    36361 tccaactgca agaatactta atgttgagga gaaaatggca ttttctctta atgcaattac
    36421 aaatattagt gcaaatatta ttaatgaatt agcttctact atccctggag aaccagctgt
    36481 ccatttaact gcattaatac acatcagaat ccacacaaaa agaattaaat ctcctgggaa
    36541 aacaaaggat tgttgtaaat gaaagattga aaagtcccag gaaatacttt ggagttcaat
    36601 attaaaaaaa gagatgtttg taagattaat aatggagaaa gaaattacta aagctgcgag
    36661 aatttggtag aggaattgaa ctttggcagg taaatttaga atatcatcaa gaaaagagcc
    36721 aactattaaa ataccaagag ctattaatat tg
//
LOCUS       DAOQWP010000005        34928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_1435_length_34928_cov_7.745018, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000005 DAOQWP010000000
VERSION     DAOQWP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 34928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34928
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_34928_cov_7.745018"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PKI16_01170"
     CDS             complement(<1..794)
                     /locus_tag="PKI16_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HNR53080.1"
                     /translation="MFSSNHSSYVAPKGWKPKNPDEDYIISFLDIELADGIGMEKFED
                     VAAACAAESSTGTWTGVYSGKNSGVKMADKMKAIAFDLEPETKTFKIAYKKELFELGN
                     MSGLLAGVVGNIDGMKMLKAFRCLDIRFPKAIVQSFPGPQFGISGVREQMQLEEGPLL
                     CTVPKPKVGRTAKEQAELAKILFTAANGGYQGIKDDENLTSLFFNKFEDRCDLVHAVQ
                     RDIEKKSGKKKFYLCNVTHSNFDTMIERTDRIKAQGGRWMMLDVVVT"
     gene            878..1879
                     /gene="rlmN"
                     /locus_tag="PKI16_01175"
     CDS             878..1879
                     /gene="rlmN"
                     /locus_tag="PKI16_01175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNR53081.1"
                     /translation="MFKKFVEKYNLPSFRLKQLQEQYFIHNISSWDELTTWSKDMRET
                     LKKEVPFSKLGNLQEFSSSDGRTVKLLSYTYDGYPVETVLMRNKERITICISSMSGCP
                     VGCKFCATGRMKNNRILDEQEIIDQVMYFKRKLAKKNESVRNIVFMGMGEPLLNLGNV
                     LNAIRILTDPELIGMSRRRITLSTVGLIEPLKEFLSANTGVKLAVSLHAPNQRLREYL
                     MPKVAKDNKLEDLMKVLIDYQKKKGKKVTYEYILIRDVNDSVEHAKELAKLLRNQICL
                     VNLINFNKSDNIPFEATTKRRVEDFQTILNSRDINNTLRYSHGNDILAACGQLANSK"
     gene            complement(1872..3227)
                     /locus_tag="PKI16_01180"
     CDS             complement(1872..3227)
                     /locus_tag="PKI16_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNR53082.1"
                     /translation="MPKYKGICFDLDTTLANTEDSSISIVKRKGLDEKSSVEYKLYEY
                     LSQYIRSISWEEFITKYDEAKSQIKETLAGTAASHSKYLYIQRVLELLGARFNPSVIY
                     EATNYYWEYVLDNMALYPGVKETLELIKKNNVKVAIITDADADIQNRKLIKLGINKYI
                     DYLVTAQEAGADKPNVKQVELALEKMNLKKDEVIVIGNNPKTDIAVAKNSHMHSILFD
                     FDHLYEDKEDDFEQHISDFKELEKLLGLERRKYVESSLVVFDLVGTMTKERHLVSTLL
                     AEILRREKEDIRPSYELYKEGKVRKEEFWKSFGIDEYDEYEDRVIAGLGIDENMLKVI
                     KDLNEKGFKLTILSNIPKEWGYAFLKKHDLGDIFPKVFFSGDLGMTKPNAKIYELLMN
                     TYLDVDPERVFCVDDDLEDLAQGKNFLMQTVWVKNVDLDYPYVPDYVMEKPGEILSVV
                     T"
     gene            complement(3256..3329)
                     /locus_tag="PKI16_01185"
     tRNA            complement(3256..3329)
                     /locus_tag="PKI16_01185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3293..3295),aa:Pro,seq:cgg)
     gene            complement(3320..3508)
                     /locus_tag="PKI16_01190"
     CDS             complement(3320..3508)
                     /locus_tag="PKI16_01190"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNR53083.1"
                     /translation="MLKKFFKDRWIIIVALIYLILPIDFISDAIPLLGNVDDFTLLLA
                     GLIKEYVDFKKEKKNTGV"
     gene            complement(3526..>4904)
                     /gene="argS"
                     /locus_tag="PKI16_01195"
     CDS             complement(3526..>4904)
                     /gene="argS"
                     /locus_tag="PKI16_01195"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNR53084.1"
                     /translation="LYTNIVGESFARLSEALGATVKRANYQGDVGLHVAKTLWGMVKM
                     MKENGVSFVDVEQKSLIERVKYLGEAYMLGFKYYDDDKDDAAIKEIDAINYYIFSMFV
                     PQLEQKDVPAYEGVDLKDMYQKGRLWCLDYFETIYERVGTTFDYYFFESEVGESGLKV
                     VLDNVGSIFKEDDGAVIYEGDKEKGLHTRVFINKYGIPTYEAKDLGLAIKKSELVEYD
                     ESIIITGNEQSGYFKVLLDALSKLRKDLAEKTVHMSHGMVKLPGAEKMSSRKGKIIDA
                     QWLLDETKEKVLAIMEKNGKDEKGKIAEKISQAAIKYAFLKVGVGKDVVFDFEKSISF
                     DGDTGPYLLYVYARGNSLLKDSGTKNLGNLCVGSCLGNPYAKELLGHIGKYGSAVLNS
                     GMSYSPSILCQYLFDLGQKFNNFYQNVRILDAQDDDRVFLLSLVEATMVIMKEGLNLL
                     GIEVVDHM"
     assembly_gap    4905..5004
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5005..5450)
                     /gene="argS"
                     /locus_tag="PKI16_01200"
     CDS             complement(<5005..5450)
                     /gene="argS"
                     /locus_tag="PKI16_01200"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNR53085.1"
                     /translation="MDIKKKIEGMIERALKEMGVQYDIVEVEEPRMSGNGDYSSNIAM
                     RLSSTLKKNPLDIANDIVSKIEKDEDIEKVEAAKPGFVNFFVSNQHLLKELSKVKSGE
                     FTSIGGKDGKKIVIEYTDANPFKILHIGHLYTNIVGESFARLSEALG"
     gene            complement(5469..5545)
                     /locus_tag="PKI16_01205"
     tRNA            complement(5469..5545)
                     /locus_tag="PKI16_01205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5509..5511),aa:Arg,seq:tcg)
     gene            complement(5559..5632)
                     /locus_tag="PKI16_01210"
     tRNA            complement(5559..5632)
                     /locus_tag="PKI16_01210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5596..5598),aa:Arg,seq:gcg)
     gene            complement(5666..6478)
                     /locus_tag="PKI16_01215"
     CDS             complement(5666..6478)
                     /locus_tag="PKI16_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR53086.1"
                     /translation="MRVLITILKFILKNWIVILFVLITTFMQHSKYSGRPVVVFLDIG
                     QGDATLIQQDSFQILVDGGPDDAVLYELAKYIPIYDRNIEVVVLTHAHDDHLKGLLMT
                     LENYNVDSIWYSPSCTETSSYQFLLEEYAEELVKVDSSTKLRYGDIAMSVIYPVEAKC
                     EENINNESVVLDVEVNGKRILLTGDAEKEVEEEIFDDIGDIDILKAGHHCSRTASSEM
                     FLNSVEPEIAICSVGEGNKFGHPHNETLEMFKKLNVQYLITYEEGNIVFELE"
     gene            complement(6441..8045)
                     /locus_tag="PKI16_01220"
     CDS             complement(6441..8045)
                     /locus_tag="PKI16_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNR53087.1"
                     /translation="MFHPLKIFTQRFVSLLAYVWDYKIILISVLHLFFILFGIPLLRN
                     NWSTPTIGLFYMLGCVLLTITIALCISKRQKDKSFVGRDDRGWRERVRQSMSIFLLAF
                     CVATFGLVCSWMVVNKEKIVSNTAMSFTSSYTEFDGVVVSEPTQKHSYQNLEIRLLRG
                     SDTELEIPGNILIKVEKFQKFRVGDVCKISGTFAEPENSENFDYKKFLKNRGIYFLME
                     YPKVECKNFNKKYFLRRWLVDLKQKIIKNVEKYLNEPQSSLFVGILMGENRLFSGSFG
                     SYVRIAGISHIVAASGYNVTILLIVLNSFLRFLPKRLRIGISLDLIWCFCLLSGVSAS
                     IVRACIMASISLIGLFWGRKGSIHVVFFLCIFIFILFNPKIVFDVGFQLSCCATGGLI
                     YLLPSIINALKIGSKEGFFVDTVLTTISCTITTLPISIFTFQTFSMWAVVANTLVLPV
                     IESTMLFGVLGLVLSSHFLMYIVNLQLKYVEIVVQLIGNSGFGYWDLVDGRGIAIAIA
                     IVIVLFCIYFYPVGDESSNYYLKIYS"
     gene            complement(8047..8703)
                     /locus_tag="PKI16_01225"
     CDS             complement(8047..8703)
                     /locus_tag="PKI16_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNR53088.1"
                     /translation="MNRRYIYVFIMLFLLGFFLGALAVYYLLPKLGKISNNLKEQSVY
                     IVENKCKPVEEEKPKCPIRVDISGAVNVPSVYCFDEGSIIMDAISTSNGLKKGYAKEY
                     LSRKINLALPLRNNQKIYIPFEKDVDCKLQEFTVIAKDVEDNFNNTETNTDVGNEGTA
                     CVNINTATKEQLTTLNGVGDSTAQKIIDGRPYTQITDLLNVSGIGQATLDKFKDKICV
                     "
     gene            complement(8739..9395)
                     /locus_tag="PKI16_01230"
     CDS             complement(8739..9395)
                     /locus_tag="PKI16_01230"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNR53089.1"
                     /translation="MSGVKDGLKDNKKILLLSVGLLVATVIGLSFLRDSSTDTNKLIN
                     TDTMQFTKPENVLEKGIDYKAVIRTSVGDITIDLFETETPNTVNSFLFLVGQRYYEGV
                     TFHRVIKNFVIQTGDPTGTGSGGPGYTVDDEITSRKYKPYTLGMANAGPDTNGSQFFI
                     TSAGIPQMNMSSLDGNYTIFGEVVEGFAVVDSIERVTTDSNDKPVNDVTVESIQILEN
                     "
     gene            complement(9435..10145)
                     /locus_tag="PKI16_01235"
     CDS             complement(9435..10145)
                     /locus_tag="PKI16_01235"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNR53090.1"
                     /translation="MKQLDITKHKTILTNILIDIYKEDLLGSSLGFKGGTAAMLFYNL
                     PRFSTDLDFDLLNNDGNTKDVIRTMTQLLSKKYDVKEQIEKYNTLFWLVSYGEGLTNI
                     KIEVSTREKPFDTYDIKTLYGIKLKVSKIGDMIANKMVATTERAVTANRDLFDIHFFL
                     SSIYVNNINYDIIKYRTGKEPVEFYTFLYGIVSNIENKNILDGLGEVLNDGQKDWVKS
                     KLKIELLGLIQRQIDMVL"
     gene            complement(10142..10711)
                     /locus_tag="PKI16_01240"
     CDS             complement(10142..10711)
                     /locus_tag="PKI16_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53091.1"
                     /translation="MNIANKLRKYDRTTTVFTISDIKTILSINDSKTVYNALFYATKR
                     QELYKISNGIYSYDQSYSRKELGNKLRTPSYISLYTVLQEEGVIFQPYSSIFLIANRS
                     EKININGQEYIYRKIKDGILLNPLGIREKEGTNIATKERAICDKIYLDGLEYFDNTEN
                     IDWNMMNQLNNEIYSNNSNISKFISAYKK"
     gene            complement(10780..16290)
                     /locus_tag="PKI16_01245"
     CDS             complement(10780..16290)
                     /locus_tag="PKI16_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNR53092.1"
                     /translation="MKKSALKNRIKSFYGKHKMAISVVFLSLQLAIGLTIYFLFGRLS
                     PNDRISVPEKDSVAFLVNENPKFSVNFGTVEEPEKQIVRFEAETSRKNPFESEDGNIF
                     SKINGLFQKKERYGIEMSLVGVNLEKTDLEKYGEEVVKVADVIGTDNVKTSTELIDVG
                     REIGKYNDDGPVSKQTVLNKEVADGVDLEYQILTGLGLKEEIIIRDLEAYKESCKDSG
                     CKLPLNEFVFDLNVDEGVEIQEGWFTIDGKSTSTYYFVDSKGRYIAHFLPNFAIDNVG
                     YKTSDVDLKVEKGEGDNYRAKVTVDTDWLFSSERVFPVRIDPSIVHDDAEDFSGGIFN
                     RTESVTGPKIQKVRDTEAFDCTGGTVTTSGEYKIHTFTSNGTLTCNTSGNVEVLVVGG
                     GGGGNSYYARNYGGGGGGAGGAVYNASFAISAGNIGVTVGNGGAVGTNGNNSVFSTIT
                     AYGGGAGGRNSNGAAGGCGGGAASTNHTGGTASQGYRGGNSTSYMGNAGGGGMGSAGV
                     DLYAIGHGTNGGTGVTYSISGSSVCYAGGGGGGAQRNLYGEIGYGQCGGGNGGGGSSS
                     VNPTAGTANTGGGGGGGAGYNASYGIGAAGGSGVVIVRYIPGEEKIITSGEYTSSSID
                     MGSSISEATLSWTPSGVNTGDGETPYSTTGLVAQWNFNETSGTTAVSGGTCGTSCNGT
                     LTNFASTGSQDAAPGTGWTANNKRWGAGALMFDGSDDYVSVVDSAGIQFGTGNFTFET
                     WVNPTVVNPGTSAQGTIISKNYTGFELSIYQGYLLAYLGGTSNSLTGTTLLKTSTWYH
                     IVLVRNGSTVSTYINGILDASTNNSASVSNIGTNLYLGQRPGGDSTKVLNGTIDSTRI
                     YSRALSANEILSNYQAANIEFQYRVSTDNSTWSNWTSYGTSASDLAWESFDNPYLYGT
                     TDTGLVAYWPMDEASGTSVADVKGSNTGTATGTKIIDGKYSKARSFNGSSDYISVAST
                     FGLGTTSYSIEAWVNIPSTSDHGCFIKIGDSTSGVCVGVGSGTTETNGNKLILLFENV
                     RWIDTGVTIGIGWHHISMIVNGSGNPLAYIDGKLVYSDVGSTPLTPVGSITGIGNYGG
                     GGSNRAFNGTIDEVRIFNIALSATSIMSDYTEGIVDKSSTISQSGSSLNIEGTGSTKL
                     ASGPYGIDSNTVGLWHLDERGGSGAYLKDSTTNSNSGTPTGTTYTNVGKIGGARSFNG
                     SSDYIGVSSPTNIPIGNSPYTIEAWIYPTATGAYGIVGWGTYGTTNWCNALRLYSSTQ
                     IVNYWWGNDLIVTVGTITTSTWHYVVATFDGTTRKIYFDGVLYGSDTPTGHAVPSANN
                     FRIGSTNNAEFFPGKLDEVRISNIARTANEIAESYKLGKDSYINQTLNTTDLSTKNTM
                     AINIASDRPGTYLSTMWGESAFANYQPDINTVGLWHMNEIAGAGAYIKDVSGYGNNGT
                     PIGTTYTNAGKIGGARSFNGSSDYISVASTFGLGTTSYSIEAWVNIPSTSDHGCFIKI
                     GDSTSGVCVGVGSGTTETNGNKLILLFENVRWIDTGVTIGIGWHHISMIVNGSGNPLA
                     YIDGKLVYSDVGSTPLTPVGSITGIGNYGGGGSNRAFNGTIDEVRISNASRTADQIRQ
                     AYEVGLRTHDVNIDFGASLVSGNLIVGSGDTSFSIDATVYGLDDLGSGIYKDEKIIVK
                     ENYDGTEYIAQGTISTITETTGAVTVSSWDSGSTFPSGGYTVNADVFKWQKEYIPIKN
                     RTVSSHVDAVNLLTFRITNGDQGRNVWIDDLRSSSGYLSAYTGEELTFPSSGRYFQYK
                     AIFSTWDNAVTPYISQVQLDYFSGPTNEQLMRHGKWFDSSGTRKGFWWVGTH"
     gene            complement(16295..18724)
                     /locus_tag="PKI16_01250"
     CDS             complement(16295..18724)
                     /locus_tag="PKI16_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53093.1"
                     /translation="MRKSNCPKVAKKLIIFFVIFLFAGVLPEGVFAVTNNVINFQGKI
                     VRNNSGYEGLNVVAGTPSCVVAGASNDTCDFRIRYYNASTSGTLLLTETFSNKEIGAY
                     NGAFDLSLGSTSPTAGSYATFDAMIKGENTIYVELGFDPAGGGSFTETFTRMPLGASA
                     FAIRAKYAAQASSAFQFDTATNGTGYSTPSEGMVFYDSDDDVLMVYNGSSWEEVGGGG
                     TGSLFTDGDIFTYLTSLTDHFVLGASSYTAIGSDSYSTYLSGLGGRATFSLDMTAERL
                     TLSGSAVRSGLTVYSDYNSTGAWPVVSFKAEDSGFDNVVLEITQDGTGDLMSLKKGST
                     DAFIFENALTMYMRQRPDAPSTVTDRLYNINGTLYWNGSPVSTSSSLWTDGDTFTYLT
                     STTDDVIIGGNSALSAKFFFDVSTGNLGIGTSTPGAKLDIAGTSSTISNTTGDITITP
                     DDTVVIKANDGEADNLMEWQNSSATILSLINQNGYATFGKATGNTGAMLTLGANTTSV
                     AQLNLTASAAVNVSAPSSGDMWWNGTNLYFNNGIANENLLNSVKIAPSVAQVDAGTST
                     SSIWINKTGTGGNLIKMQKSGSDMFVVDSGGNATFGGDSSGNVTLTLGEGIGLQGAIR
                     YNATSELLEFYINGAWLPLGYIDNKITLSAEYPGAVLSADGTSNVGVMTSDSEGTASN
                     SMNYYEWSSSETSLNDYDVRVRFTLPNDFYSWSTGGITFNFVTEATGTTNNKLDFYVY
                     LESSATVDGSSTGQASSSAGVWKTTTIAGSGLNECTGAGTTCMLIIKMSSVNDSYTRV
                     GDIDIQYNRSL"
     gene            complement(18850..21282)
                     /gene="leuS"
                     /locus_tag="PKI16_01255"
     CDS             complement(18850..21282)
                     /gene="leuS"
                     /locus_tag="PKI16_01255"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNR53094.1"
                     /translation="MKSTYNPKDFEKKWQDRWFNNPFYESKDFDNKQKYYLLLEFPYP
                     SGDSMHIGHTRNDSMMDSVARLRRMMGYNVMFPIGWDAFGLPTENYAIKVKRPPQEIS
                     KENIDTFRAQIKRLGISFDWSREVDTSDPSYYKWTQWIFLKLFEKGLAYKAEKPINWC
                     PKCKTGCANEEVVDGKHERCGTDVERRNISQWVLKITEYAEQLDKDLDLTHYVESVKS
                     RQRNWIGRKTWYDIDYKVEGTDETVRVSTTRPDTQFGASFVVIAPDHEIVERLLTHIS
                     EEKKADVQEYIIETKRKSDLERVAGEKEKSGVFTGLYCLNSMTGAKLPIYLADFALST
                     VGTGMVVGVPAHDKRDFEFATKFNLPVIRVIEGPNGDRGIVDSVEKVFEDDGMVYNSG
                     FISGLTTQEAKKKVGEYLKENKIGDVVTRFHLQDWIFSRQHYWGEPIPIIHCEKCGMV
                     PVPEDQLPVELPVVDSYEPTDDGKSPLSKIHQWVNVKCPNCGGDAQRETDTMPNWAGS
                     SWYFLRYADPKNSDIFASKKLQEYWMPVDHYEGGPEHVTLHLLYSRFWHKFLFDQNLV
                     TTPEPYDIRTIHGMVLGEGGVKMSKSLGNVISPDSLIDEYGADVTRGYMMFMGPYEGD
                     VEWSTETINGVKRFVSKYYDFLLKIWEGKIDRSGEKESKAVSRLVKRVENNILSFKFN
                     TTISALMEFYNEFSQSKFSVEDIERLVITIAPIFPHIAEEVWNITGHDYSVHMQEWPK
                     VDEKLLREEFTEVPVQVNGKVRGYISVGTADSEESVKDNVLKNTALREHIGDKEIRKL
                     IYVPKRIVNVVI"
     gene            complement(21413..21489)
                     /locus_tag="PKI16_01260"
     tRNA            complement(21413..21489)
                     /locus_tag="PKI16_01260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21453..21455),aa:Ala,seq:ggc)
     gene            complement(21503..21575)
                     /locus_tag="PKI16_01265"
     tRNA            complement(21503..21575)
                     /locus_tag="PKI16_01265"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21540..21542),aa:Ala,seq:cgc)
     gene            complement(21605..22945)
                     /locus_tag="PKI16_01270"
     CDS             complement(21605..22945)
                     /locus_tag="PKI16_01270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNR53095.1"
                     /translation="MEEDNIEQNRNISGLSFKDLTTQEQELILSEGNNEGFLFVNDDI
                     ENKKFLKVFFSKLRIFLKKQSTKKNLFAFVIALVGYFYSRVRVIWILTSVFFDLILGA
                     TEFVKERLVRRMFWGRGNFLGSALQAVLFIIVLILTISYIYRKPVVIVASGDTLDTIG
                     VAETDVLVMNATTNTAVPKDRPRRYVEQYTVKGGDTLSKIAQYYEIKVETLLWANNLN
                     EKSVIKPGTVLQIPPRDGVLVVVKKGDTLEGLAKKYSADAADIVDHNWLEKPYTLTVG
                     SELFLPNGELPVVKKPVYASSPSLYRTSSIAYTSTAVDPNVGKFLSWPVGGASAISRG
                     FFPGHYGIDIYPTGGKRPNVIAASGGRVISAGWGSGKYYGFGYYAHIDHGNGYTTLYA
                     HMNRLYVRAGQSVSRGTALGLIGDTGVAYGTHVHFELRRGAELSGRINPRPYMK"
     gene            complement(22988..24253)
                     /gene="rho"
                     /locus_tag="PKI16_01275"
     CDS             complement(22988..24253)
                     /gene="rho"
                     /locus_tag="PKI16_01275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNR53096.1"
                     /translation="MTKENKTIRDYEQMTLAQLRDEAKTLKMEDYMEFQKRELILEIL
                     KKSSEDDGFVFVDGILEVMKDESHGLLRIEGLLPSDNDIYVSGSQIKKFSLRTGDEVS
                     GPARLPKEKEKYLSLLRVDTVYGKPAAEAANRPLFRKLTPIFPNKQIKLETDQEIIST
                     RMIDLLAPIGYGQRSMIVSPPKAGKTFLLKDIANGIAANHPKAKLMVVLVGERPEEVT
                     DMKRFVKGEVYASNFDELPEQNCRIAEMALQKAMRMVEWGEDVIILMDSITRLARAYN
                     ITMPTSGKTLSGGFDPVALYPPKRFFGAARNFEEKGSLTIVATALVDTGSRMDDLVYE
                     EFKGTGNMELHLDRKLANRRIYPAIDVVNSGTRNEEFLLSKEVLNQSWRIRRMLEVLD
                     SNESPTEVLVSRLKKTKSNEEFLATLHEA"
     gene            complement(24749..26098)
                     /gene="radA"
                     /locus_tag="PKI16_01280"
     CDS             complement(24749..26098)
                     /gene="radA"
                     /locus_tag="PKI16_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNR53097.1"
                     /translation="MKYICSACGTEFLKWSGKCSSCGEWGTLEEYEEKVEVIGEAKPA
                     LFSSISEVANVKRIGTEKVLGERISTGFKEMDRVLGKGLVKGEVVLISGEPGIGKSTL
                     LMQIVLKNLKDKRILYVCGEESPGQLYSRLKRLSGEKPSSKLEANFLVTEDTVVERVV
                     ELIRESHFDMVVVDSIQSMSSSASKSYPGSISQVRICGALLTRVSKSTGIPMFIVGQI
                     NKGGDIAGPKVLEHIVDCVLYIEGDQFNQYRILRGIKNRFGSTNEIGVFEMKGDGLAE
                     VGNPSLLFLETDLKSSGSAISAVLQGSRVVFVEVQALVVEREGEAGPLRRVANGIKKP
                     RLDMLCAVMSRKGGVFLGDKDVFVNIVGGLNVNDPAVDLAICAAIKSSVKDIPLDNDC
                     IYFGEVGLTGEIRGTWGLPSVMEEAVRSGYSKIVVGRAKIGKENKLEVKKISRIREL"
     gene            complement(26103..28601)
                     /locus_tag="PKI16_01285"
     CDS             complement(26103..28601)
                     /locus_tag="PKI16_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNR53098.1"
                     /translation="MSSEILKKEEDILSRLSKNAKISLRNASLIAEHFKVKQISPEHL
                     FIGIVLNEESLASKIIEEMGIDRNELLKESFGGKLIEITISTGQKRELEISPEVKHII
                     RKAYDYSRRMSHVYVGSEHIMMAILESSDDYIKSLEKVGLTYRSFRKTFQNFAIYPLG
                     ILARPSMRVSNMEEESVVEMLGVNLVELAREGKLDPVIGREEELKNLINILSRRKKNN
                     PIIIGEAGVGKTVLVEGLAQKIADGLVPPSLRDFNIISLDISSIMAGSKMRGDVEEKV
                     MSIVEEVISSNNKILFIDEIHNIIGSNYQGGSLDIASILKPALLQDGFRCIGATTMED
                     FSAYFESDNALARRFQPLVLEEATVEDTIEILRNIKPILEKHHNVKIGEEAIKLSAVL
                     SDRYVNNRYLPDKAIDLLDEASAAKRLEIEEEFSDVSKLEDVVADIEKRKEREILNGN
                     MAKAELLEMEKKKLMQKIESMEKKWSKKRLSKANEIGAEDIRVVISKWTGIPVNTLGA
                     KEISSLVKLESQLGSKVIGQEEACKIVASAIKRARTGISDTDRPWASFLFLGPTGVGK
                     TELAKVLAQELFGNQDRLIQIDMSEMMEMHSVSKLIGSPPGYIGFQQGGWLTEKVRTN
                     PHSVILFDEVEKAHQDVLNILLQILEYGHLTDGRGRRVNFKNTVIILTSNIGAEEIKK
                     NKVLGFVGKDTEKSDEDIDRAYSSMKTQLLLELKNTLRPELLNRIDDIVIFRSLTRKD
                     ARKIVKLLIKELNGRLAQEKVSVTLDSKAITYIVKEGFSEEYGARPIKRLLQDTVENA
                     VANYLLEKGKIGNGLKNMEIKIGMKDNKISVLNK"
     gene            complement(28625..29863)
                     /locus_tag="PKI16_01290"
     CDS             complement(28625..29863)
                     /locus_tag="PKI16_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNR53099.1"
                     /translation="MVNKKTDNAQYAQIDQFLADQSHKIKKLSKGDIIEGDVVDVRDS
                     YLVVDVGHKSEGIVAGRELKSDSFNWKDLKVGDKVLVYVVKPEDEEGQLVLSIRRTQQ
                     ASAWITLEKAKKENDVVEAVVVESNNGGLIVEIGKDIRGFIPTSQLDASRVYLDGVRQ
                     VGKDISSRVQKRLNSLVGEKIQTRIIELDREKNRIILSEKMVTQSRDLEQREKTLNKV
                     KEGDVLEGIVSGITPFGIFVNAEGLEGLVHLSELSWDKVEDIGKIYAVGSKVKVMVIG
                     ISDGGKRVAYSIKRLLQDPWSAAISKYKIGDVVKGTIQKVVPYGAFVRIGEGLNGLIH
                     ISELSSQLVKNPEDIVKVGDKVDVKILSISSTERHLGLSLKAIGSKSEKIKEKNEEIS
                     QEELATAVDSAVEAELESKE"
     gene            complement(29940..30566)
                     /gene="nth"
                     /locus_tag="PKI16_01295"
     CDS             complement(29940..30566)
                     /gene="nth"
                     /locus_tag="PKI16_01295"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNR53100.1"
                     /translation="MNKKELAKQLLLEIEKIYPDASTELKNWKTPFQFLVCIILSAQT
                     TDKQVNKVTKKLFGKYPDAKSLSIADIDDITNIVKSVNYFKTKAKHIIETAKLLQNNY
                     NGVPPKDVVELQKLPGVGYKTANVFLNDLYKSNQGIAVDTHVVRVARTYGLTKNVNPT
                     KIAHDLEKLYPKRDWYKVNVFFVLYGRYILKAKKRIEEKVVLKEEIVL"
     gene            complement(30559..31581)
                     /locus_tag="PKI16_01300"
     CDS             complement(30559..31581)
                     /locus_tag="PKI16_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53101.1"
                     /translation="MEGENYGVKTVAIEGGDQVGKGDAVLNLSRALVEEGESITMLAL
                     PFYALPIGMSIRKMICTNQDELKKIEGLENVVGTPRQTEIAMAMFALNRLEVLKTLEK
                     AEGLVMLDRSAFSHALTISYNIFLGNISKENMEEFAYKGIDMDQLFIDKLNLSNCVLN
                     LRTEKSKWNASRGNGEDMYEREEVQDICDSVYDIFKEYSKEGWKNVVTRKDDKWRSRE
                     DILRENLEFVHSRISIKRGNRGNLRVLSAKDISNDIYPSIRLSSKEEELWMEAVLAND
                     KQKMYKASVNIAEEIAKKIKRIQLSSEIKEQFKKILDGYPEVYSLLEAFAGSRYVLVL
                     REAVNE"
     gene            complement(31968..32083)
                     /gene="rrf"
                     /locus_tag="PKI16_01305"
     rRNA            complement(31968..32083)
                     /gene="rrf"
                     /locus_tag="PKI16_01305"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(32215..>34928)
                     /locus_tag="PKI16_01310"
     rRNA            complement(32215..>34928)
                     /locus_tag="PKI16_01310"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT        10097 a         7909 c         5270 g        11552 t
ORIGIN      
        1 gtaacaacaa catccaacat catccatctc ccaccttgtg cctttatacg gtctgttctc
       61 tcaatcattg tatcgaaatt ggaatgtgtg acattgcata ggtaaaactt cttttttcca
      121 gattttttct caatatccct ttgtactgca tgtacaagat cgcatctatc ttcaaatttg
      181 ttaaaaaaca aacttgttag gttttcatca tccttaatac cttgatatcc tccatttgca
      241 gcagtgaaaa gtatctttgc taattctgcc tgctcctttg ctgttctccc gacttttggc
      301 ttaggtacag tacaaagaag aggtccctcc tcaagttgca tctgttctct tacaccacta
      361 ataccaaact gtggaccagg gaaagattgc acaatagctt ttgggaagcg aatatccaaa
      421 catctaaacg ctttcagcat cttcatccca tcaatatttc ccactactcc tgcaagaaga
      481 cctgacatat tgccaagctc aaaaagctct ttcttgtagg ctatcttgaa tgttttagtc
      541 tcaggctcaa gatcaaaagc tatcgctttc atcttgtcgg ccatctttac acctgagttt
      601 tttccagaat atacgccggt ccaagtaccg gtagaacttt cagctgcaca tgcagctgct
      661 acatcctcga acttttccat tccgatacca tcggcaagct cgatatctaa aaaagatata
      721 atgtagtcct catcgggatt ttttggcttc catcccttag gagcaacgta tgaggaatga
      781 tttgaactga acattggcag atttttaaat gttatatatc tccatattag aataaaccgc
      841 tccttttgta aaatacctat ctaggatata atgcggtatg tttaaaaaat tcgtagaaaa
      901 atataatctc ccttccttta gactaaaaca gttacaagag cagtacttca tacataatat
      961 ctcctcttgg gacgagctta ctacatggtc taaagatatg agggaaacat taaaaaagga
     1021 agtccccttc tcaaagcttg gaaacttgca agagtttagc tccagcgatg gaagaacagt
     1081 taagcttctc tcctacacat atgatgggta tccagtagaa acagttctga tgagaaacaa
     1141 agagagaata accatttgca taagcagtat gagtggttgt cctgttggat gtaaattctg
     1201 cgcgacagga aggatgaaga acaatagaat ccttgatgag caagagataa ttgaccaggt
     1261 tatgtatttc aaaagaaaat tggcaaagaa aaacgagagt gttagaaata tcgtttttat
     1321 gggtatggga gagcctttac ttaacttggg caatgtcttg aacgcaatta ggatacttac
     1381 agatccagag ctaattggaa tgagtagaag aaggattacc ctttctacag ttggattaat
     1441 tgaacctcta aaagaattcc ttagtgcaaa tacaggggta aagttagcag taagtttaca
     1501 cgcaccaaac caaagattaa gagaatattt aatgccaaaa gtagctaaag acaacaaatt
     1561 agaggattta atgaaggttt taattgacta ccaaaagaaa aaaggtaaga aggttacata
     1621 cgagtatatc ctcataaggg atgtaaatga ttcagttgaa catgcaaaag agttagcaaa
     1681 gttacttaga aaccagattt gtttagttaa tctgattaat tttaataaat cggacaatat
     1741 tccttttgaa gctacaacaa agagaagggt tgaggacttt cagactattc ttaattcaag
     1801 agatattaac aatacactga gatattccca tggtaatgat attttagccg cttgtggaca
     1861 gttggcaaac tctaagtaac tacactcaga atctctcctg gtttctccat tacataatct
     1921 ggaacatacg gatagtctag atctacgttt tttacccaaa ctgtctgcat taagaagttt
     1981 tttccctgtg ctaagtcttc taaatcatca tctacgcaaa aaaccctctc agggtctaca
     2041 tcaagatagg tattcatcaa taattcatat atctttgcat tgggctttgt catacccaga
     2101 tctccactaa agaagacctt ggggaagata tctcctaaat catgcttctt taagaaagca
     2161 tacccccatt ctttcgggat atttgaaaga attgttaatt taaaaccttt ttcattcaaa
     2221 tctttaatta ccttaagcat attctcatct atacccaaac ccgcaattac cctgtcttca
     2281 tactcatcat actcatctat accaaaactc ttccaaaact cttcttttct taccttcccc
     2341 tccttgtaaa gctcgtagct tggtcgaata tcttccttct ctcttcttag tatttctgcc
     2401 aaaagagtgc tcactaagtg cctttccttg gtcattgtac cgaccaaatc gaagacaact
     2461 aagctactct ctacatactt gcgtctttct aatcctaaaa gtttctctaa ttctttgaaa
     2521 tccgagatat gctgttcgaa atcgtcctct ttatcttcat acaggtgatc aaagtcaaaa
     2581 aggatggaat gcatatgact attcttagct actgctatat ctgttttagg attattaccg
     2641 ataactataa cctcgtcttt ctttagattc attttctcca atgccaactc aacctgttta
     2701 acatttggct tgtctgcccc tgcttcttga gctgtaacaa gataatctat gtatttattg
     2761 ataccaagtt ttattaattt tctattttgt atatccgcat cagcatcagt tataattgct
     2821 actttaacgt tgttcttctt tattaattct agtgtttcct taactcctgg ataaagagcc
     2881 atattatcaa ggacatactc ccaatagtaa tttgttgctt cgtatataac cgaaggatta
     2941 aaacgagcac caagaagctc aagaactctt tgtatataca gatatttact gtgagatgct
     3001 gcagtacctg ccagtgtttc cttaatctga cttttagcct catcgtattt tgtaataaat
     3061 tcttcccaac ttatacttcg tatatactgg gacagatatt cataaagttt gtattccact
     3121 gaggatttct cgtctaagcc ctttctttta acaatggaaa tagaagaatc ttcggtattg
     3181 gcaagggttg tatctagatc aaagcagata cctttgtatt taggcatttc tttataatat
     3241 ataaaataat atatatcggg gtgactcgat ttgaacgagc gacctctacg tcccgaacgt
     3301 agcgctctag ccatctgagc tacaccccgg tattcttctt ctccttcttg aaatctacat
     3361 actctttgat taaaccggct agtagcaaag taaaatcgtc tacattaccc aacaaaggta
     3421 ttgcatcgct tataaaatca atcggaagta ttaaatatat caatgcaaca atgataatcc
     3481 acctatcctt aaaaaatttt tttaacataa ttgttttcta ataaattaca tatggtccac
     3541 aacctctata cctaagagat ttaatccctc cttcataatt accatagtag cctctactaa
     3601 actaagaaga aatacccggt catcgtcttg ggcatcaagt atacgaacat tctggtaaaa
     3661 gttattaaat ttctgtccta aatcaaacag atattggcaa agtattgatg gagaatagct
     3721 catgccagag ttaagcacag cgctcccgta cttgccaatg tgaccaagca actcctttgc
     3781 atagggattt cccaggcagc tcccaacaca caggtttcca agattcttag taccagaatc
     3841 tttcagaagc gaatttcctc tcgcataaac atagaggaga tatggtccag tatctccgtc
     3901 aaaactaata ctcttttcga agtcgaagac aacatcttta ccaaccccta ctttcaagaa
     3961 agcgtatttt atagcggctt gggatatctt ttcagcaatt tttccctttt catccttacc
     4021 gttcttttcc attatggcaa gaactttttc cttggtttca tccaaaagcc actgtgcatc
     4081 tatgatcttt ccttttctac tagacatttt ctctgcacct ggaagcttaa ccataccatg
     4141 cgacatatgg actgttttct ctgcaagatc ttttcttaat ttgctcaatg catctaacaa
     4201 aactttgaaa tatccagatt gctcgttacc agtaatgatt attgactcat catactcaac
     4261 taattcactc ttttttattg ccaaccctaa atcctttgcc tcgtaagtag gaattccgta
     4321 tttgtttata aaaacccttg tgtgaagacc tttttctttg tctccctcat atataactgc
     4381 accgtcatct tccttaaata tgctccctac attgtccaaa acaaccttca aaccactttc
     4441 tcctacctca ctttcgaaga agtaatagtc aaaagtggtt cctactctct catatatcgt
     4501 ttcgaagtaa tccaaacacc acaatcttcc cttctggtac atatctttaa gatctactcc
     4561 ctcatacgca gggacatctt tctgttctaa ctgaggaaca aacattgaaa agatatagta
     4621 gttaatagca tcaatttcct taattgctgc gtcatctttg tcatcgtcat aatatttaaa
     4681 tccaagcata tatgcctctc caaggtactt tactctctca attaggctct tctgttctac
     4741 atccacaaaa gaaacaccat tctctttcat catcttgacc ataccccaca aagttttggc
     4801 aacatgaaga ccaacatccc cttggtagtt agccctcttt actgtggctc ctagagcctc
     4861 tgaaagcctc gcaaaagact ctccgactat gttggtatat agatnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnncctaga gcctctgaaa gcctcgcaaa agactctccg
     5041 actatgttgg tatatagatg accaatatgt agtattttaa aaggattagc atctgtatat
     5101 tcaattacaa tcttcttacc atctttacca ccaatacttg taaattcccc gctcttaacc
     5161 ttacttaatt ccttaagcaa atgctggttt gacacaaaga aattcacaaa tcctggctta
     5221 gcagcctcta ccttttcaat atcctcatcc ttttcaatct tgcttacaat atcgttcgca
     5281 atatccagtg gattcttctt taaagtagaa gacaatctca tggcaatatt gcttgaataa
     5341 tcgccgtttc cactcattct tggctcctct acttcaacga tatcgtactg tacacccatc
     5401 tcttttagag ctctttcaat cattccctct atttttttct taatatccat atttttattc
     5461 taacataatg gcgcactcgg agggattcga acccccaacc gtcggttccg aagaccgatg
     5521 ctctatccag ttgagccacg agtgcatttt taaatatacg tacctggccg gaatcgaacc
     5581 gacaaccgtt ggctccgcaa gccaatgctc tatccaattg agctacaggt acaaaagagc
     5641 tatgaatgta ttaattctac cacatttatt ctaattcaaa tacaatattc ccctcctcat
     5701 atgttattaa atattgcaca ttaagctttt taaacatttc caatgtttca ttgtgtggat
     5761 gaccgaattt gttcccttca ccaaccgaac agatcgctat ctcaggttcg actgaattga
     5821 gaaacatctc tgaggaagca gtccgagagc agtgatgacc agctttgagt atatctatat
     5881 ccccaatgtc atcgaaaatc tcctcctcta cctctttttc agcatcccct gttaatagaa
     5941 ttctttttcc gttcacctct acatccaaaa ccacagattc gttattaata ttttcctcac
     6001 actttgcctc tacaggatat attacggaca tggctatatc tccatacctt agcttcgttg
     6061 aggaatctac ttttacgagc tcctctgcat attcttcgag tagaaattgg taagaagaag
     6121 tctccgtaca cgatggggaa taccagatac tatctacgtt ataattctca agggtcatta
     6181 gtagcccctt caggtgatca tcatgagcat gcgttagaac aactacctca atgtttctgt
     6241 catatatcgg gatatatttc gctagctcat acaaaacagc atcatctggt ccaccatcca
     6301 ccaaaatctg gaaactatcc tgctgtatga gagttgcatc gccctgcccg atatcaagga
     6361 atacgactac cggcctacca ctatacttgc tatgctgcat aaaagtagta attaggacaa
     6421 aaaggattac aatccagttt ttaagaataa attttaagat agtaattaga actctcatct
     6481 cctacaggat aaaaatatat acaaaacaat acaataacga tagcaatcgc tatggcaatt
     6541 cccctcccat caaccaaatc ccaatatccg aaaccactat ttccaatcaa ctgcactact
     6601 atctccacat atttaagctg taagttaaca atatacataa gaaaatgact agaaagaaca
     6661 agtcctagca caccaaacaa catagtcgat tcaattacag gcagtactag agtattggca
     6721 acaacagccc acattgaaaa tgtttgaaag gtaaaaatcg agattggcaa tgtagtaatc
     6781 gtacagctga tagtagtcag aaccgtatcc acaaagaagc cctctttaga accaatcttt
     6841 agcgcattaa taatcgatgg aagaagatat ataagaccac ctgtagcaca acatgacaat
     6901 tgaaacccta catcaaagac aatctttgga ttaaatagta tgaaaataaa aatacagaga
     6961 aaaaatacca catgtatact cccctttctt ccccaaaaca gaccaattaa agatatgctc
     7021 gccattatac aggccctcac tatagatgca gatacgccag agagcaagca gaagcaccat
     7081 attagatcta aggatatgcc aatccttaat cttttaggca agaacctcaa aaacgaattc
     7141 aacactataa gcaagatcgt tacattgtat ccagaggctg caacaatatg acttatacct
     7201 gcaatgcgta cataagaacc gaaagatccg ctaaataacc tgttctcacc cattaaaatc
     7261 cctacaaata gggaagattg aggctcattt aggtattttt ctacattttt aataattttc
     7321 tgcttcaaat ctaccaacca tcttcgcaaa aaatattttt tattaaaatt cttacactcc
     7381 acttttggat actccattaa aaaatatatc cctctgtttt ttaaaaattt tttgtaatca
     7441 aaattttcgg aattttctgg ttcggcaaaa gtaccagaaa ttttacacac atctcctact
     7501 ctaaattttt gaaacttttc tactttaatt aaaatatttc ctgggatttc aagctctgta
     7561 tcactacccc gtagtagacg tatctctagg ttctggtagc tgtgcttttg ggtaggttcc
     7621 gataccacta ctccatcgaa ttccgtgtaa ctcgaagtga aactcatcgc tgtatttgaa
     7681 acaatttttt ccttgtttac caccatccaa gagcagacca gtccaaaagt agctacgcaa
     7741 aaggccagaa gaaatatact catggactgc ctcactctct ccctccaccc cctgtcatcc
     7801 ctacccacaa aactcttgtc tttctgtctt ttggagatgc acagagctat cgttatggtt
     7861 aacaaaacac acccaagcat gtagaacaat ccgatagttg gtgtactcca attgtttctc
     7921 agaagaggta tcccaaagag aataaaaaag agatgtaaaa cagaaatcaa tatgattttg
     7981 taatcccata cataagcaag aagacttaca aacctctgag taaaaatttt aagaggatga
     8041 aacatttcaa acacatattt tgtctttaaa tttgtccaat gtggcctgcc caattccact
     8101 aacatttaac aagtccgtta tctgtgtata tggtcttcca tcaattatct tctgtgccgt
     8161 agaatctcct actccgttta atgtggtaag ctgttctttg gttgctgtat tgatgtttac
     8221 acacgctgtt ccttcgtttc ccacatctgt attcgtttct gtattgttga agttgtcctc
     8281 tacatctttt gctataactg taaactcctg tagcttacaa tctacatctt tctcaaaggg
     8341 gatatatatc ttctgattgt ttctaagagg aagcgctaag ttaatcttcc tagagagata
     8401 ctccttcgcg taacctttct taagaccatt agaagtgcta atagcatcca taattatgga
     8461 tccttcatca aagcagtaca cagaggggac atttacagca ccagagatat caacccttat
     8521 cggacatttt ggcttttctt cttctacagg cttacattta ttctctacaa tgtatacact
     8581 ttgctctttt aggttattgg atatctttcc aagctttggt aagaggtaat acacagcaag
     8641 ggcaccaagg aaaaagccaa gaaggaaaag cattataaat acatagatat atctcctatt
     8701 cataggagat aatatatgat agcgagatta aaaaacgatt aattctccaa aatttggatt
     8761 gactctacag tgacatcatt gactggttta tcgttagaat cagtagttac cctttcgatt
     8821 gaatcaacga ctgcaaagcc ctctacaacc tctccaaaga ttgtgtaatt tccatccaag
     8881 ctagacatat tcatttgagg gatacctgcg ctagttatga agaactgaga accatttgta
     8941 tctggtcctg catttgccat gccaagagta tatggtttgt atttcctact tgttatctca
     9001 tcatctacag tatatccagg acctccacta ccagttccgg ttggatctcc tgtctgtatg
     9061 acgaaatttt taattactct gtggaatgta actccttcgt aatatctctg ccccacaaga
     9121 aagaggaagc tgtttactgt gtttggtgtt tcagtttcaa aaagatctat ggttatatct
     9181 ccaacggatg ttcttattac tgctttgtaa tctatccctt tctctaacac attttctggt
     9241 tttgtaaatt gcattgtatc tgtattgatt aatttatttg tatctgtaga agaatctcgc
     9301 aaaaaagaaa gacctattac cgtggcaaca agtaaaccta cagatagtag caatattttc
     9361 ttattatctt ttaatccatc ttttactcca ctcatatgta tatactaata tatttgaggg
     9421 gcagttggca atctttaaag aaccatatct atctgccttt gtattaatcc taacagttct
     9481 atttttaatt tggatttaac ccaatctttt tgaccatcat ttagtacctc acctaatcca
     9541 tcaagaatat ttttattctc tatatttgaa acaatgccat acaagaaggt gtagaattct
     9601 acaggttctt ttcctgtgcg atatttaata atatcgtaat ttatattatt tacatagata
     9661 ctgctcaaaa agaaatggat atcaaagaga tctctatttg cggttacagc tctttctgta
     9721 gtagccacca ttttattcgc aatcatatcc cctatcttcg atactttaag tttaatgcca
     9781 tacaaggttt tgatatcata tgtatcaaat ggtttctctc ttgtagatac ctctatcttg
     9841 atatttgtta aaccctctcc atagcttact aaccagaaca atgtattata cttttcaatc
     9901 tgttccttta catcatattt tttagaaagt agctgagtca ttgttctgat tacatctttt
     9961 gtattaccgt cattgttgag cagatcaaaa tccaaatcag tagagaacct tgggagattg
    10021 taaaagagca tagctgctgt tcctccctta aaaccaagac tggatcccag caggtcttct
    10081 ttgtatatgt ctattaatat gtttgttagt attgttttat gttttgttat atctaattgt
    10141 ttcatttttt gtatgcagat atgaatttag atatgtttga attgttagaa tatatctcgt
    10201 tatttaattg attcatcatg ttccaatcta tattttcggt attatcaaag tattctagtc
    10261 catctaaata tattttgtca catattgccc tttccttggt tgcaatattg gttccctcct
    10321 tctctctaat gcctaatggg tttaagagga ttccgtcttt gatctttcta tatatatatt
    10381 cttgcccatt tatattaatt ttttctgatc tatttgcaat taaaaatatt gaggaatatg
    10441 gctgaaatat cactccttct tcttgtaata cggtgtacaa gcttatgtat gatggagttc
    10501 ttagtttatt tcctaactct tttcttgaat atgattgatc atatgagtag attccattgg
    10561 atattttgta gagttcttgt cttttagtag cataaaataa ggcattgtat acagttttac
    10621 tatcattgat acttaatatc gtctttatat cagagattgt aaatacagtt gttgttctat
    10681 catattttct taatttgttt gcgatattca taacatatag tgcttaacta ttcagtactg
    10741 tatgttacaa tatgccgaat ggaaagtcaa ctggtcagat taatgagttc ctacccacca
    10801 gaagcccttc cttgttccac tgctatcaaa ccactttcca tgtctcataa gctgctcatt
    10861 ggtaggaccg gagaagtagt ctaactgtac ttggctaata tatggtgtaa ctgcattatc
    10921 ccaagttgag aatattgctt tgtattgaaa gtatcttcca ctagatggaa atgttaattc
    10981 ttctccagta taagcagaaa gataccctga ggatgatctt aaatcatcta tccatacatt
    11041 tcttccttga tctccattgg taattctaaa agtaagaaga tttacagcat cgacatgaga
    11101 acttactgtc ctgttcttaa tcggtatgta ttctttttgc catttgaata catctgcatt
    11161 tacggtatat cctccacttg ggaatgtaga gccactgtcc caagatgata cagttacagc
    11221 tcctgtagtt tcagttatgg tagatatagt accttgggct atatattctg taccatcgta
    11281 gttttctttt acaatgattt tttcatcttt gtatatccca ctccctagat catcgagtcc
    11341 gtaaacagtt gcatctatac taaaggatgt atctcctgag cctactataa gattcccact
    11401 tacaagagat gccccaaaat ctatatttac atcatgtgtt cttaaaccta cttcatatgc
    11461 ctgacgaatt tgatctgctg ttctagaagc atttgatata cggacttcat caatggtacc
    11521 attaaaggct ctatttgaac caccaccacc ataattacca attcctgtaa tgctacctac
    11581 tggtgtgagg ggtgtagaac ctacatctga gtagacaagt tttccatcaa tatatgctaa
    11641 tgggttccca ctaccattta caatcataga aatatgatgc caccctatac caatagtaac
    11701 acccgtatct atccatcgaa cattttcaaa gagtaaaatt aatttatttc cattggtttc
    11761 tgttgttcca gaaccaacac ctacacacac accacttgtt gaatcaccaa ttttgataaa
    11821 acatccatga tcagaagttg agggaatatt tacccaagct tcgatagaat agctcgtagt
    11881 tcccaaacca aatgtcgaag caactgagat ataatcacta cttccattaa agcttcttgc
    11941 tcctcctatt tttcctgcat tagtatatgt agttcctata ggagttccat tatttccata
    12001 cccagataca tccttgatat atgccccagc tccagctatc tcattcatat gccataatcc
    12061 aactgtatta atatctggtt gataattagc aaatgcagac tctccccaca tagtagatag
    12121 gtatgtacct ggtctatcac ttgctatatt tatagccata gtatttttag tagataaatc
    12181 tgtagtatta agtgtttgat taatatatga atctttacct aatttatatg attcggctat
    12241 ttcattagct gtacgagcta tatttgatat acgaacctca tctaactttc caggaaaaaa
    12301 ttctgcgttg tttgtagatc caattctaaa attattggca ctaggaactg catgaccagt
    12361 tggggtatcg gaaccataca gaaccccatc aaaataaatc tttcttgtcg taccatcaaa
    12421 cgttgctaca acatagtgcc aggtggaagt tgttattgtg ccgactgtaa ctatcaaatc
    12481 atttccccac caatagttca ctatctgggt agaagaatat agtctaaggg cgttacacca
    12541 atttgtagtt ccatatgttc cccagccaac aattccatat gcaccagtag cagttgggta
    12601 tatccatgct tcaattgtgt acggagaatt tcctattgga atgtttgttg gtgatgatac
    12661 acctatataa tcactactac cattaaaact tcttgctcct cctatttttc ctacattagt
    12721 atatgtagtt cctgtaggtg ttccactatt agaatttgta gtggaatcct tcagatatgc
    12781 tccactacct cctctttcat ctaggtgcca taaccctacg gtatttgaat caattccata
    12841 tggaccacta gctaatttag ttgatcctgt accttctata tttaggctac taccactttg
    12901 ggatattgta ctactcttat ctacaattcc ttcggtataa tcagacatta tacttgtagc
    12961 tgataatgct atattaaata tacggacttc atcaatggta ccattaaagg ctctatttga
    13021 accaccacca ccataattac caattcctgt aatgctacct actggtgtga ggggtgtaga
    13081 acctacatct gagtagacaa gttttccatc aatatatgct aatgggttcc cactaccatt
    13141 tacaatcata gaaatatgat gccaccctat accaatagta acacccgtat ctatccatcg
    13201 aacattttca aagagtaaaa ttaatttatt tccattggtt tctgttgttc cagaaccaac
    13261 acctacacac acaccacttg ttgaatcacc aattttgata aaacatccat gatcagaagt
    13321 tgagggaata tttacccaag cttcgataga atagctcgta gttcccaaac caaatgtcga
    13381 agcaactgag atataatcac tacttccatt aaaacttctt gccttagaat attttccatc
    13441 aataattttc gttcccgtag ctgttccagt attgcttcct tttacatctg caacagatgt
    13501 accagaagcc tcatccatag gccaatatgc tactaatcct gtatctgtag taccatataa
    13561 atatggatta tcaaaacttt cccaagctaa atcacttgcc gaggtaccat atgatgtcca
    13621 attactccat gtgctattat ctgtacttac tctatattgg aattcaatat ttgctgcttg
    13681 ataattagaa agaatttcat tagcagagag ggcacgagaa tatatacggg tagaatctat
    13741 agttccattt agaacttttg tcgaatctcc tccaggtctt tgtccaagat atagattcgt
    13801 cccaatatta ctgacggatg cagaattatt tgtgctagca tctagtattc cattaatata
    13861 tgtcgaaact gtagagccat ttcgtactaa tacaatatgg taccacgtac ttgtttttag
    13921 taatgttgta ccagttaaag agtttgatgt tccacctaag tatgcaagaa gataaccctg
    13981 gtatattgaa agttcaaaac cagtataatt ttttgaaatg atagttcctt gtgcagaagt
    14041 tcctgggtta acaactgtag gatttaccca tgtctcaaat gtaaaatttc cagtaccaaa
    14101 ttgaattcca gcactatcaa caacacttac ataatcatca cttccatcaa acattagtgc
    14161 tcctgctccc cacctcttgt tatttgcagt ccaacctgta cctggtgctg catcttgaga
    14221 tcctgtagat gcgaaattag ttaacgtacc attacatgat gtaccacatg tacctccact
    14281 tacagctgta gtaccactcg tttcattgaa attccattga gctactaatc cagttgtact
    14341 atatggtgtt tctccatcac cagtatttac accagatggt gtccaagaga gagttgcttc
    14401 tgagatacta cttcccatat ctatggatga agaagtgtat tccccggaag taataatttt
    14461 ctcttcgcct ggtatatatc taacaataac aacacctgaa cccccagctg caccaatacc
    14521 ataagaagca ttatatcccg caccgccacc accacctcca cctgtattgg cagtaccagc
    14581 tgttgggttc acactacttg aaccaccacc accatttccc cctccgcact gtccataacc
    14641 aatttctccg tataagtttc tttgggcacc accacctcca cctcctgcat aacaaactga
    14701 tgatccacta atactatatg tgacaccagt tccaccattg gtaccatggc ctatggcata
    14761 taaatcaaca cccgcacttc ccattcctcc accacctgca tttcccatgt atgatgttga
    14821 attccctccc ctgtatccct ggcttgcagt acctccagta tggttagttg acgcggcacc
    14881 accaccacaa ccacctgcag caccgttaga atttctaccc cccgcccctc caccataggc
    14941 agtaatggta gaaaagacgg aattatttcc atttgtgcct acagccccac catttcccac
    15001 tgttacacca atatttcctg cagaaatagc aaaagaggca ttgtataccg cacccccagc
    15061 gcctcctcca cctcctccat agttcctagc ataatatgaa tttccaccac cacctcctcc
    15121 aacaacaaga acctcaacat taccagaagt attacatgtc aatgtcccat ttgatgtaaa
    15181 agtatgtatt ttgtattcac ccgatgtcgt tactgttcct cctgtacaat caaaagcttc
    15241 tgtgtctctt accttctgta tctttggtcc agttacactc tctgttctgt taaatatccc
    15301 cccgctaaaa tcttctgcat catcatgtac aatactagga tctattctta caggaaatac
    15361 tctctcgcta ctgaataacc aatctgtatc aacagtaacc tttgccctgt agttatcacc
    15421 ctctcccttc tctaccttca aatctacatc agaggtcttg tacccaacat tatctattgc
    15481 aaagtttgga agaaaatgtg ctatgtacct tcccttgcta tctacaaaat aatatgtact
    15541 cgtactctta ccatctattg taaaccaacc ctcctgtatc tctactccct catctacatt
    15601 caagtcgaaa acaaactcat tcaaaggtaa tttacaacca ctatccttac agctttcttt
    15661 gtatgcttcc aaatccctta tgataatctc ctcctttaaa cccaagcctg tgagtatctg
    15721 gtactccaga tctacaccat ctgctacctc tttgttcaaa accgtttgtt tagatacagg
    15781 accatcatca ttgtacttac caatctctct accaacatca attaattctg tagatgtctt
    15841 tacattatca gtccctatta catctgccac cttaacaact tcctcaccat acttctctaa
    15901 gtcggttttt tctaaattca cgcctaccaa agacatctct attccatatc tttctttctt
    15961 ttgaaaaagt ccatttattt tagaaaatat atttccatcc tcactttcaa aaggattttt
    16021 cctagaagtt tctgcttcaa atcttacaat ttgcttttct ggttcctcaa ccgtaccaaa
    16081 atttacagaa aatttaggat tctcatttac taagaatgca acactatctt tctctggtac
    16141 agatattctg tcattgggag aaagtctacc aaagaggaaa tatatagtga gaccgatagc
    16201 gagctgtaaa gaaagaaaga ctacagaaat agccatttta tgtttaccat agaaactctt
    16261 tattctattt tttaaggcag attttttcat tagattacaa tgatctatta tactggatat
    16321 ctatatcccc tactcttgta tagctatcat ttactgaaga catttttatt attagcatac
    16381 aagttgttcc ggcacccgta cattcgttca accctgagcc tgcaatagtg gttgttttcc
    16441 aaaccccagc agaggaagat gcttgccctg tagaagagcc atctactgta gcagagcttt
    16501 cgagatacac ataaaaatct aatttgttat ttgtagtacc agtcgcctct gttacaaaat
    16561 taaacgttat accaccagta ctccaagagt aaaaatcatt aggaagtgta aatcttacac
    16621 gaacatcgta atcattaagg gatgtctctg agctactcca ttcatagtag ttcattgaat
    16681 tagaagctgt accttcactg tccgaagtca ttacaccaac attgcttgta ccgtctgcag
    16741 agaggacagc tccgggatat tctgctgaaa gtgtaatttt attatctatg taacccaaag
    16801 gtaaccaagc tccattaata tagaattcta gaagttcaga agtcgcgttg tatctaattg
    16861 ctccttgcaa acctataccc tctcctagcg ttaaagtaac attaccagag gaatctcctc
    16921 caaaagtagc atttccccca gaatctacta caaacatatc actaccactc ttctgcattt
    16981 taattaagtt tccacctgta ccagtcttgt taatccagat actgcttgta cttgtacctg
    17041 catctacctg tgccacagat ggagctatct tcacagagtt aagcaaattc tcgtttgcta
    17101 ttccgttatt aaaatacaaa tttgtaccat tccaccacat atctccagaa gatggagccg
    17161 aaacattgac tgcagcagaa gcagttaaat taagctgtgc tacagatgtc gtatttgcac
    17221 ccaatgttag catagcacca gtattgccag ttgcttttcc aaaagtagca tatccattct
    17281 ggttaattaa acttagaata gttgcagaag aattctgcca ctccatcagg ttatcagcct
    17341 caccatcgtt tgccttaatt acaaccgtat catcaggagt tatagttata tctcccgtag
    17401 tattggatat cgtggagctt gttccagcaa tatctaactt agctcctgga gtactagttc
    17461 caatacccaa atttcctgta gaaacatcaa agaagaactt tgcgctaagt gctgaatttc
    17521 caccaataat tacatcgtct gtagttgaag tcagataagt aaatgtatca ccatctgtcc
    17581 acagagaact tgatgtagat actggagaac cgttccaata cagtgttccg ttgatattgt
    17641 acaacctgtc tgtcacagtt gaaggtgcat ctggtctttg gcgcatatac atcgtaagtg
    17701 cattctcaaa aatgaatgca tctgtacttc ccttttttag tgacattaaa tctccagttc
    17761 cgtcttgagt aatctccaac actacattgt caaagccgga atcctctgcc ttgaaagaaa
    17821 ctactggcca tgcgccagtc gaattgtagt cagaatatac tgttagacca cttctaactg
    17881 ccgagccgga aagagttaat ctctctgcag tcatatccaa agagaatgtt gctctgccac
    17941 caagcccaga cagatatgtc gagtatgaat cactccctat tgcagtgtaa gagcttgccc
    18001 ctaaaacaaa atgatctgtt aggctcgtca agtatgtaaa aatgtcgcca tctgtaaaca
    18061 gtgaacctgt cccacctcca cctacctctt cccaggagga accattgtaa accattaaaa
    18121 catcatcatc gctgtcatag aagaccatac cctctgaagg agttgaatat cctgtaccat
    18181 ttgtagctgt atcaaattga aatgcgctac tagcttgtgc agcatacttc gccctaatgg
    18241 caaatgcaga tgctccaagt ggcatccttg taaatgtttc tgtgaatgaa ccaccaccag
    18301 caggatcaaa tcccaattcg acatatatcg tattctcacc ctttatcatc gcatcaaatg
    18361 tagcatatga accagcagtt ggagaggtag aaccaaggga aagatcaaaa gctccattgt
    18421 aagccccaat ctctttattt gaaaatgtct ctgttagaag aagtgtccca cttgttgatg
    18481 cattgtaata tctaattcta aaatcacagg tatcgttaga tgcgcctgca acaacacagg
    18541 aaggagttcc tgctacaaca tttagaccct cataaccgct attatttcta actatctttc
    18601 cctgaaagtt aattacattg tttgtgacag caaaaacacc ctctgggagg actccagcaa
    18661 aaagaaaaat tacaaagaaa atgattaact tttttgctac tttcgggcag ttagattttc
    18721 gcatttctgt caggcagtaa aaacgtccta tttaaagcaa atttagtagc ccccactacg
    18781 ataaaaatat taacggaaaa tcttttattt gtcaaacttt ttgctcaatt tttaggcata
    18841 aaaattggcc tagataacaa cgtttactat acgctttggc acatatatca actttcgtat
    18901 ttctttgtct ccgatatgct ctcgcagagc ggtattcttt aggacattgt ccttcacact
    18961 ctcctcacta tcagctgttc ctactgaaat gtatcccctt accttcccat tgacctgtac
    19021 aggcacctct gtaaattctt ctcgaagaag tttttcgtcg acttttggcc actcttgcat
    19081 atgtacgctg taatcatgtc ctgtaatatt ccagacttcc tctgctatat gtggaaatat
    19141 tggagcaata gtgataacta acctttcaat atcctcaact gagaacttac tttgagaaaa
    19201 ttcattgtaa aactccatca aagcagaaat tgttgtgtta aatttaaaac tcagaatatt
    19261 attctccacc cttttcacta atctacttac cgctttactc tccttctctc cacttctgtc
    19321 aattttaccc tcccaaattt taagcaaaaa atcataatat ttagatacaa acctcttaac
    19381 accgtttatc gtttcagtac tccattccac atcaccctcg taaggaccca tgaacatcat
    19441 gtatcccctg gtaacatctg caccgtattc atctatgaga gaatctgggc ttatgacatt
    19501 gccaagtgac ttagacatct taactccacc ctccccaaga accattccat gaattgttcg
    19561 gatatcgtat ggttctggtg tagttacaag attttggtca aaaaggaatt tatgccagaa
    19621 tctcgaatac aagagatgca atgtcacatg ctctggtcca ccctcgtaat gatctaccgg
    19681 catccagtac tcctgcaact ttttgcttgc gaatatgtcc gaattcttcg gatcggcata
    19741 tcgcaagaaa taccagctag agccggccca atttggcata gtgtctgttt cccgctgagc
    19801 gtctcctcca caattcggac atttcacatt cacccattga tgtatttttg acaacggaga
    19861 cttcccatca tctgttggct cgtaactatc taccactgga agctctactg gtagctgatc
    19921 ttctggtact ggtaccatcc cacatttctc acagtgaatt attggaattg gctcacccca
    19981 gtaatgctga cgagagaaaa tccaatcttg taaatggaat cttgttacaa catctccaat
    20041 tttgttctct ttgagatact ctcctacttt cttttttgct tcttgcgttg ttaaacctga
    20101 aatgaagcca gagttataca ccattccatc gtcctcaaag accttctcaa cagaatctac
    20161 aattccccta tctccatttg gcccttcaat taccctaata acaggaagat taaactttgt
    20221 agcaaattca aaatctcttt tgtcgtgagc tggtactcct actaccattc ctgtaccaac
    20281 ggttgaaagt gcaaaatctg ccaaatatat tggcaacttt gcaccagtca tgctgtttaa
    20341 acagtaaaga ccagtaaaca caccactttt ctccttctcc ccagctactc tctccaaatc
    20401 tgactttctc ttggtttcga tgatatattc ctgtacatct gcctttttct cctcagatat
    20461 gtgtgttagg agtctctcca ctatctcatg gtctggagct attactacaa aagaggctcc
    20521 aaattgagta tctggccttg ttgtagagac tcttacggtt tcatccgttc cctctacttt
    20581 gtaatctatg tcataccagg ttttccttcc aatccagttt ctttgtctac ttttaacact
    20641 ctctacatag tgtgttaaat ccaagtcttt gtctaattgt tctgcatatt ctgtaatttt
    20701 tagcacccat tggctaatat ttcttctctc tacatctgtt ccgcatctct catgctttcc
    20761 atctacaacc tcttcatttg cacagcctgt tttacactta gggcaccagt taattggctt
    20821 ttccgccttg tatgcaaggc ctttttcgaa aagtttaaga aatatccact gagtccattt
    20881 gtagtaggaa ggatctgagg tatctacctc tctactccag tcaaaagata tgcctaatct
    20941 cttaatctgg gctctaaagg tgtctatatt ttctttacta atctcttgcg gaggtctttt
    21001 tactttaata gcgtagtttt cggtaggcaa tccaaatgca tcccacccta ttgggaacat
    21061 tacattgtaa cccatcattc tacgcaatct agcgacagaa tccatcatac tgtcgtttct
    21121 tgtatgtcca atatgcatgc tatctccaga gggatatggg aactctagca aaaggtagta
    21181 tttctgcttg ttatcgaaat ctttactttc atagaaggga ttattaaacc acctgtcttg
    21241 ccacttcttt tcaaaatctt ttggattgta agtacttttc atactactta aggagagttt
    21301 aatacagtaa ttctacaaga aaaaggaaag cattggaaga gagggatatt gcattctgtg
    21361 gaacatgagt gtgtaacata tgttgttttg agtacaaaaa gatactcaag tatggtggac
    21421 ctgacgggac tcgaacccgt gaccccatcg ctgccagcga tgtgctcaaa ccaactaagc
    21481 tacaggccca aatatattaa catggaccca gccggaatcg aaccggcgat cttcgcaatg
    21541 cgaatgcgat gttctaccag ctgaactatg agcccacaca gaaagattat acccaaaatt
    21601 agccctattt catatatggt cttggattaa tacgaccaga taattcagca cctcttctca
    21661 attcgaaatg aacatgtgta ccgtaagcaa cacccgtatc tcctattaaa ccaagggctg
    21721 tacctctaga aactgactgc cctgctctaa catataatct gttcatatgt gcatacagag
    21781 ttgtatatcc attaccatgg tcaatatggg cataataacc gaaaccatag tacttaccag
    21841 aaccccatcc tgcacttatg actctaccac cagaggctgc tattacattt ggtcttttac
    21901 cacccgttgg atatatgtcg ataccgtaat gtcctgggaa gaatcctcta gatattgcac
    21961 tcgctccacc aactggccaa cttaagaact tgccaacatt tggatcaact gcagtggatg
    22021 tataggctat actcgaagtt ctgtacaaag aaggagaaga tgcatataca ggttttttaa
    22081 ctactggtaa ttcaccattt ggtaagaaca actcactccc aactgttaga gtatatggtt
    22141 tttctagcca gttatggtct acaatgtctg cagcatcagc tgaatatttc tttgccaatc
    22201 cttctaaagt atctcccttt tttactacaa caagaacacc atctcttggc ggaatctgta
    22261 gcacggtccc tggcttaatt acactcttct cattgagatt attagcccaa agtagtgtct
    22321 ctaccttaat ttcgtagtat tgagcaatct tacttaatgt atctccacct tttactgtat
    22381 attgctctac atatcttctt ggtctatcct ttggaactgc tgtatttgtt gttgcattca
    22441 ttaccaaaac atctgtttcg gcaactccta tggtatctag cgtatcaccg ctagcaacaa
    22501 taacaaccgg tttacggtat atatacgaga tagtgagtat aagaacaatg ataaataata
    22561 cagcttgtag tgcagacccc aaaaaatttc ctctccccca gaacatcctt cttacaagcc
    22621 tctcttttac aaactctgta gctccaagaa tcaagtcaaa aaacacagag gtcaatatcc
    22681 aaataactct taccctagaa taaaagtaac ctactaaagc aataacgaat gcaaaaagat
    22741 tcttcttggt actctgtttt tttagaaaga ttcttaactt tgaaaagaat acctttaaaa
    22801 acttcttgtt ctcaatatcg tcattaacga aaaggaatcc ctcattatta ccctcagaaa
    22861 gaatcaattc ttgttcttgt gtggtaagat ctttgaaact gagaccagat atattcctat
    22921 tttgttctat attatcctct tccactttaa tactgtttta actgatagtt ttaattatac
    22981 cagttatcta tgcttcgtgc agagtagcaa gaaattcttc gtttgatttg gtctttttaa
    23041 gtctacttac tagaacttct gttggactct cattactatc caagacttcc aacattcttc
    23101 taattctcca tgattgattc aatacttctt tacttagaag gaactcttca tttctagtac
    23161 ctgaatttac tacatctatt gcaggatata tcctcctgtt agcaagcttt ctgtccaaat
    23221 gtagttccat atttcctgtt cctttaaatt cctcatatac aaggtcatcc attctactac
    23281 cagtatctac aagagctgta gcaacaattg ttaaactacc cttctcttcg aaatttctag
    23341 cagctccaaa gaatctttta ggaggataca gcgctactgg atcaaaacca ccagagagtg
    23401 ttttacctga tgttggcata gtgatattgt atgctcttgc aagtctagta atactatcca
    23461 taaggataat gacatcctca ccccactcaa ccattctcat agctttctga agtgccatct
    23521 ctgcaattct acaattttgc tcaggaagtt catcaaagtt ggaggcataa acctctcctt
    23581 ttacaaacct cttcatatct gtaacctctt caggtctctc tcctaccaac acaaccataa
    23641 gctttgcttt aggatggtta gcagcgatac catttgcaat atctttaaga agaaatgttt
    23701 ttcctgcctt tggaggcgaa acaatcatgg atctttgtcc ataaccgata ggagcaagaa
    23761 gatctatcat tcttgtagat ataatctctt ggtctgtctc aagcttaatc tgtttgtttg
    23821 gaaatattgg tgttaatttt ctaaaaagag gtctgttggc agcctctgca gctggttttc
    23881 cgtatacagt atcaactctc aaaagactta agtatttctc cttttctttt ggaagccttg
    23941 ctggtccaga aacctcatct ccagttctta gactaaattt cttaatctga gaaccagata
    24001 catatatatc attatcactt ggaagaagtc cctctattct taatagtccg tgggactcat
    24061 ctttcataac ttctaaaata ccatctacaa agacaaatcc atcatcttct gaagattttt
    24121 ttaaaatttc taatattaac tctctctttt ggaattccat atagtcttcc atcttaagtg
    24181 tttttgcttc atcacgaagc tgagcaagag tcatttgctc ataatctcta atcgttttat
    24241 tctcttttgt catttttaca gggaaaatat ataaagtatt gagcctaaat cccaatctac
    24301 aatttcagta tattgaaatg taaaaaacat gtcaaatcga aaaagtactt gagataaaaa
    24361 aagggatcgg tactgcccca accgaccccg gtttcttcct ccatatagaa gaagatatcg
    24421 agtaacatat cgaaatatgt taatcccttt tcttcaaaga ctaagaaatc ttaggctttt
    24481 cctgttgccc agatgtgctt gtgacacccc agacaaaggg aaaaaactaa gattgtccac
    24541 accatctttg aaagagcgta taaagtatac ctcatacaaa gataactgtc aaccctatat
    24601 catctataca caaaacttgt ctctttcgag atatcttact gcatagacat ttctgcatgc
    24661 tcctctatcc tttctgttac gattatcacg gccaccctcc cctgttacga tatttgtgga
    24721 ttgttgagaa gaaaataaaa aagagaggct aaagttccct tattctggag atttttttaa
    24781 cctctagctt gttttccttc cctattttag ctctcccaac aacaatctta gaatagcccg
    24841 accttacagc ctcctccatc acggaaggaa gtccccaggt acctctgatt tcgccagtaa
    24901 gccctacttc tccaaaatat atacagtcat tatccaaagg aatgtctttt actgacgatt
    24961 ttatagcggc acatattgcc aaatctactg ctggatcatt tacatttaat ccacctacta
    25021 tatttacaaa cacatcttta tctccaagaa atacaccacc ttttcgcgac attacagcac
    25081 aaagcatatc caaacgaggc ttctttatac cgttcgcaac tcttctgaga ggaccagcct
    25141 caccttctct ctcaactaca agggcctgca cttcaacaaa cacaactctt gagccctgca
    25201 atactgcact tatagcacta ccagaagact taagatctgt ttcaaggaaa agcaaagatg
    25261 gattaccaac ttcagccaag ccatctccct tcatttcaaa cacgcctatt tcattagtag
    25321 agccaaacct atttttgata cccctaagaa ttctgtattg attaaactga tctccctcta
    25381 tatatagtac acagtcaacg atatgctcaa gtacctttgg acctgctata tccccgccct
    25441 tgttaatctg accaactata aacattggaa tacccgttga tttagagacc cttgtgagaa
    25501 gtgcgccaca tatccttact tggctaatac ttccaggata actcttggat gcagaagaag
    25561 acatactttg aattgaatcc acaactacca tgtcgaaatg actttcccta atcaattcca
    25621 ccactctttc gaccacagta tcctctgtca ctaagaagtt tgcctctaat ttactagagg
    25681 gcttttcccc agatagccgt tttaacctag agtaaagctg tcctggggac tcctctccac
    25741 aaacatacag gattcttttg tccttaaggt ttttaagtac aatttgcata agaagagtgg
    25801 attttcctat tcctggctct ccactaatta gcacaacctc tccttttacc aacccctttc
    25861 ctagaactct atccatttcc ttaaatccag tagatattcg ctcacccaag accttctctg
    25921 tacctattct ctttacattt gcaacttcag atatactgct aaacagggca ggctttgctt
    25981 cacctattac ctctactttt tcctcgtact cttccaaagt tccccattca ccacaggaag
    26041 aacactttcc agaccacttc aaaaattcag tgccacatgc agagcatatg tatttcattt
    26101 atttatttgt ttaaaacaga tattttatta tctttcattc ctatttttat ttccatgttt
    26161 tttaatccat ttccaatctt ccctttctct agtagatagt tagcaactgc gttctcgaca
    26221 gtatcttgca gaagtctttt aattggacga gcaccatact cttcgctaaa accctcttta
    26281 acaatatatg tgattgcctt actatctaaa gtcacagata ccttctcctg tgcaagcctt
    26341 ccgttaagct ctttaattag taatttaact atctttctcg catctttgcg cgtaagagat
    26401 ctgaatatta caatgtcgtc tatacgattg agaagctctg gtctaagagt atttttcaat
    26461 tctaatagaa gctgtgtttt catagatgaa tatgccctat ctatatcctc atcacttttc
    26521 tctgtatctt ttccaacaaa accgagtact ttgttctttt taatttcctc tgcaccaata
    26581 tttgaagtaa gaattatgac cgtatttttg aaattcactc ttcttcctct accatctgta
    26641 agatggccat actctaatat ctgtaaaagt atatttagta catcctggtg tgctttttct
    26701 acttcatcaa aaagtattac actatgtgga tttgttctga ctttctctgt aagccatcct
    26761 ccctgttgga aacctatata tccaggagga gagcctatca atttggagac actgtgcatt
    26821 tccatcattt cactcatatc tatctgtata agtctgtctt gattaccaaa taattcttga
    26881 gctagtactt tcgctaattc tgtcttacca actcctgtcg gacctaagaa aagaaatgat
    26941 gcccacggcc tatccgtatc tgaaattcct gttctagccc tctttattgc ggatgctact
    27001 atcttacatg cctcttcctg tcctattact ttgcttccta attgactctc aagtttaaca
    27061 agagatgaaa tttcttttgc tccaagagta tttactggaa ttccagtcca tttggatatg
    27121 actacgcgga tatcctcagc gcctatctcg tttgctttac ttaatctctt ctttgaccat
    27181 tttttttcca tgctttcaat tttttgcatt aactttttct tctccatttc caaaagttct
    27241 gcttttgcca tattcccatt taatatttct ctctcttttc ttttttcaat atctgcaact
    27301 acatcctcca actttgatac atctgaaaac tcttcctcta tctcaagcct ttttgctgca
    27361 gaagcctcat ctagaagatc tatggccttg tctggaagat atctgttgtt gacatatctg
    27421 tcagagagta ctgcagaaag tttaattgct tcttccccta tttttacatt gtgatgtttc
    27481 tctagtatgg gcttgatatt tctcaatatc tctatagtat cctcaacggt tgcctcttca
    27541 agaacaagtg gttggaatct tcttgccagt gcattatctg attcaaagta tgcagagaaa
    27601 tcttccatag ttgttgctcc aatgcaccta aatccatctt gaagtaatgc tggcttaaga
    27661 attgatgcta tatctaaaga gcctccttgg taattagagc caataatatt gtgtatctca
    27721 tctatgaaga ggattttatt gtttgaagat attacctctt ctactatgga catcaccttt
    27781 tcttctacat cacctctcat cttacttcca gccataatgc tggagatatc tagggaaatt
    27841 atgttgaaat ctcgcaatga tggaggaact aatccgtccg caattttttg tgctagtcct
    27901 tctactagga ctgtcttacc tacacctgcc tcccctataa ttattgggtt gtttttcttt
    27961 cttctactta aaatatttat gaggttcttt aattcctctt ccctacctat tactggatct
    28021 agtttacctt ctctcgcaag ctctacaaga tttactccaa gcatttctac aacagattct
    28081 tcttccatat ttgaaactct catagatgga cgtgcaagta tgccaagtgg atatatggca
    28141 aaattttgga atgtttttct aaatgatctg tatgttaaac caaccttttc taaagatttt
    28201 atgtagtcat cactagattc aagtatagcc atcattatat gctctgagcc cacatataca
    28261 tgagacattc ttcttgagta atcgtatgct ttgcgtatga tgtgtttaac ttctggggag
    28321 atttctaact ctctcttttg acctgtacta atggttatct ctatgagctt acctccgaag
    28381 gattccttta gcagttcatt tctatctatt cccatttcct caattatctt gcttgcgaga
    28441 gattcttcat taagtactat gccaataaaa agatgctctg gtgaaatttg tttaacttta
    28501 aaatgttctg caataagaga agcatttcta agagatatct tcgcattctt acttagcctt
    28561 gaaagtatat cctcttcttt tttaagtatt tctgatgaca taccctatta tactatattc
    28621 tcggctattc ttttgattct aattctgcct ctactgcgct atctactgca gttgcaagct
    28681 cctcttggga aatttcttca ttcttttctt ttattttttc actcttgcta cctatagctt
    28741 ttaagcttag gcccaagtgt ctttctgtag aggatatgga aagaattttt acatctactt
    28801 tatctcctac ttttacaata tcttcaggat ttttgacaag ctggctagat agttctgata
    28861 tatggataag accattgaga ccttccccaa ttcttacaaa tgctccataa ggaactactt
    28921 tttgaattgt acctttaact acatccccaa ttttgtattt agatatcgca gcagaccatg
    28981 ggtcttgaag taatctcttt atactgtaag ctactctctt accaccgtct gatattccaa
    29041 ttaccattac cttcacctta cttccaactg catatatctt accaatatcc tcaactttgt
    29101 cccacgacaa ttctgaaagg tgaacaagtc cttcaaggcc ctctgcatta acaaagattc
    29161 caaaaggtgt aattccacta acaataccct caagaacatc tccctcttta actttgttta
    29221 gagtcttttc tctctgttcc aaatctctag attgggtaac cattttttcg gaaagtataa
    29281 ttctgttttt ctctctgtct aattctatta ttcttgtttg aatcttttct ccaactagag
    29341 agttcaacct tttctgaact ctggatgata tatcttttcc aacctgtctt actccatcaa
    29401 gatatactct ggaagcatcc aactgtgaag ttggtataaa tccccttata tcttttccaa
    29461 tctctactat taatccacca ttgtttgact caacaactac tgcctctact acatcattct
    29521 ctttctttgc tttctctaat gtaatccaag cactagcctg ttgtgttctt ctaattgaaa
    29581 gaacaagttg tccttcctca tcttcaggct taacaacata gacaagaacc ttgtctccta
    29641 cttttaaatc tttccaattg aatgaatcac tctttaattc tcttccagca acaataccct
    29701 cagatttatg accaacatct actactagat agctatctct aacatctact acatctcctt
    29761 caatgatatc tccttttgaa agttttttta tcttatgtga ttgatccgct agaaactgat
    29821 caatctgtgc atactgggca ttgtctgttt ttttatttac catagggtta tcctttggtg
    29881 atgtaaaacc tgcagttggt tattacatac aactaatatt atatctactg cggtctggat
    29941 tatagcacga tttcttcttt caagacaacc ttttcctcta ttcttttctt agctttgagt
    30001 atataccttc catagaggac aaaaaagaca tttactttgt accaatctct ctttggatac
    30061 agtttttcaa ggtcatgggc tatttttgta ggattaacat tttttgttaa tccatatgtt
    30121 cttgctaccc tcactacatg ggtatctaca gcaattcctt ggtttgattt gtacaaatcg
    30181 tttaggaata cattggcagt cttgtaacca acaccaggta atttttgtag ctcaacaaca
    30241 tcttttggtg gtactccatt gtaattattc tgaagcaatt ttgctgtttc tattatatgt
    30301 ttggcttttg ttttgaagta gtttactgat tttacaatgt tggttatatc atcaatatct
    30361 gcaatagaaa gagatttagc atctggatat tttccaaaaa gtttctttgt taccttatta
    30421 acctgtttat ctgtagtttg tgcagagagt atgatacaaa caaggaattg aaagggtgtt
    30481 ttccaattct taagttcagt agaggcatct ggatatatct tctctatttc aaggagtaat
    30541 tgttttgcta attctttttt attcatttac tgcttctctt aatactagta catatctgct
    30601 tccagcaaaa gcttcaagaa gactatacac ctcggggtat ccatcaagta tcttcttaaa
    30661 ttgctctttt atctctgatg ataattggat cctttttatc ttcttagcta tttcttccgc
    30721 aatatttaca gaggctttgt acattttttg tttgtcatta gccaaaacgg cttccatcca
    30781 caattcttcc tcttttgagg atagcctaat gctgggatat atatcgtttg agatatcctt
    30841 tgcagaaaga acccttagat tacctctatt accccttttt atggatattc tactatgcac
    30901 aaattctaaa ttctctcgaa gtatatcttc acgacttctc cacttgtcat ctttgcgtgt
    30961 tacaacattt ttccatcctt cttttgaata ttctttaaaa atatcatata cactatcgca
    31021 tatatcctga acctcttccc tctcgtacat atcctcgcca ttgcccctcg aagcattcca
    31081 tttagatttt tctgttctca aatttagaac acaattggaa aggtttagtt tatctatgaa
    31141 gagctgatcc atatctattc ctttgtatgc aaattcctcc atattctcct tggagatatt
    31201 gcctaaaaat atattgtaag agattgttaa tgcatgacta aaagcgcttc tatccagcat
    31261 tacaagtcct tcggcttttt ctaatgtttt taaaacttct aatctattca atgcaaacat
    31321 tgccatggct atctctgttt gtcttggtgt tcctacgaca ttttctagac cttcaatttt
    31381 cttaagttca tcttggttcg tacaaatcat cttacgaata ctcatcccaa ttggaagagc
    31441 gtagaatggt aaggcgagca ttgttatgga ctctccctct tctactaaag ccctcgaaag
    31501 attcaaaacc gcatctcctt ttcctacttg atcacctccc tctatcgcga cagtcttcac
    31561 cccatagttt tcaccttcca tttttatacc aacttttcat attttatata tgattttatt
    31621 atatacctcc atcacagcct tcggagagaa caaaatagtt actctggcga tagtgtgtat
    31681 ttacagtcat ttctcaaagg gggtgtaccc gagtgctttt gtaagaattt tcttaatcaa
    31741 ttttctagtc ttaaaatcca tagtctcaga ctctctgtta cgatttatcg ctctaccccc
    31801 gcccgatgtc tccaacacaa tagttctttt gtaacttcgg aacaaaaatc ttccgactca
    31861 tttagccttc gttccaacct tattccaagc ccaatagtat attctattgg tcataattta
    31921 gaaaacaaaa aacccactct ttcgagtggg ttatcaaatt caataagtcc tggcaatgac
    31981 ctatcttccc aggaccaaag gtccaagtat tttcggcgct agagcgtttt acttctgagt
    32041 tcgggatgga gtcaggtagt tccactctgc tgcaattgcc aggcataaaa gatctatgtc
    32101 taaatctctt gtgcctgact accttacttg cgtaagatat tcaggactta aattctaaag
    32161 agcgatagag tatgtacaat gtacacacta aaaactgaat agaaggtgta atttcaaaag
    32221 aagctttcga tcgattagta ctccttggct aaacacattg ctgtgcttac acctagagcc
    32281 tatcaaagtg gtaatctacc acagatcttc cgtacagtaa actgtacaag tatatttaat
    32341 cttaggaatg gtttcgtact tatatgcttt cagtacttat ccagaagaaa cttagctact
    32401 cagcgatgca cttggtagta cagctgatac accagaggtt tccaaaccct gatcctttcg
    32461 tactaagggc tgaatccttc aaatatacaa cgcctatacc ggatagagac cgacctggct
    32521 cacgacggtc tgaacccagc tcacgtgccg ctttaatgga cgaacagtcc aacccttggg
    32581 accttctcca gtcccaggat gcgacgagcc gacatcgagg tgccgaacct tgccgtcaat
    32641 atgaactatc gggcaagact agcctgttat ccccggggta gcttatctcc gttaaacaat
    32701 gacgcttcca cgcgcaatca aagggtcact taaacctact ttcgtaactg atcgggatgt
    32761 ctccctcaca gtaaagcctc cttatgctta tgcactcttc acccgatttc catccgggtt
    32821 gaggagacct tagtgcgctt gcggtactct ttagcaagca accgccccag tcaaactgcc
    32881 caccatgcac tgtcgagttt ccagataatg gaaacagtta gaaacaaaaa cacaaaagag
    32941 tggtattaca ctgacgactc cgccatccct gacgagatga cttcaaagtc tcccacctat
    33001 tctcttcatt cgaatcttta ttccaatgca aagttgcagt aaagctccac ggggtctttt
    33061 tgtcctggta taggtaggcc gcatcttcac agccatttca atttcaccga gcgcgccgtc
    33121 gagacagagg aagagtcgtt acgcctttca tgcaagtcac taattaagtg acgaggtatt
    33181 acgctacctt aggcattcct gttacttata tctagtcata ttaatatact agatgaccta
    33241 atatatatta ttaggtggac gcaccttcgt gcatctctct acattgctgt agaggtcgga
    33301 ctatatcttc acaactacag ttgtgtctag cgtttagcct ctgaggattc ttgtaactat
    33361 attctctttg ctccactttt taccttttcc ttgctgatta atctcatatg ctaaatctac
    33421 caaatggatc attccatttc ttgttagatg ttgctttgaa ttcataaaag aaactccttc
    33481 ttcaaatata ttgaaggcct ttgattttac aatcaaagga tatttcttaa agaaagggat
    33541 tactttagta taacaaggtt taataccaga aatctgatat gtaaatacat tatacggaga
    33601 tgtttttctg tgaatacttc cacatccaaa catctgtctt atcatctcca atatttcgat
    33661 gcccttttca tgttgataaa cataaaaggc tggttctatt ctcaaaccat atttaacatc
    33721 ttgtctattt ttgacattaa gacaaaagct tccctctcca tctataaatc cggagagaaa
    33781 ccatttcaat tcattagtaa aagcagaact agaagttaca agcctttcct gctgattgtc
    33841 caaagacata agattgttac tatttaagta cttatgttgt ttaggatgtt ccagcatata
    33901 gctagatttt acaacggccc tatatctgat agcaaaccaa agtatcaaaa gatacctcag
    33961 tagatatata tctaccaccg ttatagttac agccgccatt gaccagggct tcagcttcac
    34021 gcttcaccac cactagggca ccgaagtgca cccagtagga agttaacgca aaaccttaac
    34081 cttctggcat tgggcaggcg tcagcactta tacatcttct tacgaatttg caagcgcctg
    34141 tagttttggt aactagtcgc tcctccctat tctgtgccac cttcatcgtt actagaaaaa
    34201 atcttgtaac ggaaggtccc ccttcttgcg aacttacggg gtgaatttgc cgaattcctt
    34261 aacaacgcat cactcgtacg ctttggtgca tttacaccag tccaccagtg tcggattgca
    34321 gtacggttac tgatattttt gtttaccgaa gtaaactgct agaagttttt cctggagaat
    34381 taatatgacg gattggattc ccgaaggtcc cctttgtatc acagctcgtt ttcattccac
    34441 aaagtggaaa acattttccc ggatttacct aggaaaacta actaactgct tgcacctcaa
    34501 accaattaga ggcccgcctt ttcaattctc aagctccatt gcaaatatca atagtacagg
    34561 aatattaacc tgttgtccgt cggatacgct tttcagcctc tccttaggac cgactaaccc
    34621 atggttgact gccatagcca tggaaacctt agacattcag cgagcagggt tttcacctgc
    34681 tttacgttac tcatcccggc attctcactt ctatacgctc cagcagctcc tttcgggcca
    34741 ccttcacagc taattatcaa atgctaattt gatcatgtat agaacgctcc cctaccactt
    34801 aaaaatcata aatgatagat aaatttgcag cttcggaata catcttcagc cccgttgaat
    34861 cttaagcgca gaatctcttg actagtaagc ttttacgcac tttttaaagg atggctgcct
    34921 ctgagcca
//
LOCUS       DAOQWP010000006        30662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_1760_length_30662_cov_7.381318, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000006 DAOQWP010000000
VERSION     DAOQWP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 30662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30662
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1760_length_30662_cov_7.381318"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /gene="topA"
                     /locus_tag="PKI16_01315"
     CDS             complement(<1..1954)
                     /gene="topA"
                     /locus_tag="PKI16_01315"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNR53102.1"
                     /translation="MTKSTKKYKNLLIVESPAKAKTINKYLGDDFKVMATIGHIIDLP
                     KSKLAVDVEDGYKPLFETIYGKGKVIQDLKKAVPKDGHVYLAMDPDREGEAIAWHVDS
                     VLKAKNTKRVTFHEITKDAILEAVSKPSDININLVEAQKARRVLDRLVGYKLSELVWK
                     KIWYGLSAGRVQSVALRLVVEREEERDAFDSEEYWEFFVDTTSPSGDTLRAKLVKKDG
                     KKYLPVKAEEVKDIEEFLGKGSYEVVDVLKKNIKRHAYPPFTTSTLQQSANNILGYSS
                     KRTMGIAQVLYQAGVITYMRTDSVNLSSKAIDGIRELVKNKYGVEYLPEQANYYKTKS
                     KNAQEAHEAIRPTDFSLEKAAIDSKFGPAEAKLYDLIWRRAISSQMKEKEAETLTIHI
                     ESKDKKKPLYTFSLGGEKTLFDGFRRALGGGKGDSDLQEVSKIAKGDILKWDQFVKEQ
                     KFTQPPARYTEAALVKKLEELGIGRPSTYATIISTIQSRGYVTKEEKALLPTDVGKVV
                     TNLLRNNFKRLVDYKYTAKVEDELDDIANGKSEYIPVIEAQYKPLVAEIEKALKDVNK
                     EDIVILSKSKEKCPVCGSEMLVRVGRYGKFLTCSKFPECKGMKDLSGGEESLDFKKYA
                     KPEKCPKCGSKLLLKNSKYGKFWACEK"
     gene            complement(2055..2726)
                     /gene="dprA"
                     /locus_tag="PKI16_01320"
     CDS             complement(2055..2726)
                     /gene="dprA"
                     /locus_tag="PKI16_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNR53103.1"
                     /translation="MYIIKAADKEYPGNLRNIYRYPNVLYCKGNKEILSRRLITIVGT
                     RAMSTYGGWVVRNLLSSVPKELNVAFVSGLAIGIDSQVHKMCLEKGLATIAVIAGGID
                     KGFPAQNREIYEEICKKGTVVAEFPEGTETRKEMFPIRNRILAGMSDTTIVIEGGLDS
                     GSLLTANLALEYGRDVFVVPGDITRESSKGCNSLLKQGADIITGVEDFLDILGIDGEQ
                     IRIGI"
     gene            complement(2762..4762)
                     /gene="mrdA"
                     /locus_tag="PKI16_01325"
     CDS             complement(2762..4762)
                     /gene="mrdA"
                     /locus_tag="PKI16_01325"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNR53104.1"
                     /translation="MELKLSKKMKNSIPRRKKQASKGRSEPFSFSVKKYFSKPTAVEK
                     GSPWNYIFVFFIFFILFVVLLGNLTTLQIVKGKEMLDKSNSNQIKVKTETAYRGAIFD
                     RNGKKLAENIPSMNLYIKMEDYLDDKGAIKAEMLENTANTLGGMLGDMWKKTFAGGEY
                     GSILEKIYSVHKQSPYFTDILIAENISNEKAIEIKSKSESLIGIYIDNDSQRYYPYKD
                     IISHIIGYTGDVTGEDLKSFESLALTDTIGKSGVERWYDEQLRGENGEIAIEVDALGR
                     SVSNQEYVIKEAVSGDSLYLTIDIDIQRKFYDFLKSGVRKAGAVGGAGIIEDVETGEI
                     IAMVTYPSYDNNLFVGGISTKNYSKLLNNKNLPLINRAISVQMPPGSTFKSLVAPAAL
                     DAGAITKNTIYVSRYGYKFSNGAPFQEFQNHAYGALNVVGGIAQSSNIFFCELIRNWD
                     INKLDPYLEKFGIGKYTGIDVPGEAPGRLPSPANKKLLAETTSPWLESVWYPEGDSCN
                     SVIGQGITLVTPIQMSNWMAALATEGKLHTPHVAKSFRDEQGETETLTFDLLEENMVS
                     KEAFNITKEGMWSAVNGPKRSVGQLANVGVVVAAKTGTAEFGKVNSKGIYENTHAWVG
                     GFFPYNKPKYSFSVFLEDGGHSSNAVAIMQSMIMWMVKAGKI"
     gene            complement(4753..5256)
                     /locus_tag="PKI16_01330"
     CDS             complement(4753..5256)
                     /locus_tag="PKI16_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53105.1"
                     /translation="MLKVLYIIIGIIGALFIEGFLLSAFGIRIFFVLILLLLGRLDIK
                     WLSGILVIYALISDVVFHYPLGIDILLVGIPLLLLFGSSFIFNINEGVVGYGIKLITI
                     ALYFVLILVLPPLFLNGSFGILTWHMVLILGIKSVLLVIVLYIMDILLSFTRGKENNI
                     KISKKWN"
     gene            complement(5268..6095)
                     /locus_tag="PKI16_01335"
     CDS             complement(5268..6095)
                     /locus_tag="PKI16_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNR53106.1"
                     /translation="MNAQGNFKKELIFTIIVILFGGILLSLDKANVTNFIRYPISYSM
                     EPIFITGEGLGKGISSYFKNFVSLRDIVEENERLKIQIAENEIGEASYLEILAENELL
                     KKQLSLKNKGREYVMGNVLKSDGIDYLRIDEGTKSGIQVGDVAVVGNFFVGVVEYVDE
                     LGSRIKLPTSNNSYLGVSILKYDESRTLDGYLKNKVISRGVMNGSAEGIKVENITMNS
                     DINDGDIIFVNDSSVGDMLVLGYVVGLSSNPASISRTCFVSTIVDYDTLSKVFVRIN"
     gene            complement(6112..7167)
                     /locus_tag="PKI16_01340"
     CDS             complement(6112..7167)
                     /locus_tag="PKI16_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR53107.1"
                     /translation="MKLINSILEFFTYDLGIDLGTANTIVYMRNHGIVVSEPSVVAID
                     KRTKTVLAVGKDARQMIGRTPANISAVRPLRDGVISDFDTTQAMIHHFIHRAHGYFGK
                     SFKLPRPRVIIGVPTSVTEVERQAVLDAAKTAGAREVFIVEEAMAAAIGAGLPVEDAS
                     GSMIVDIGGGTSDIAVFSLGDIVVDNTMRIAGDEMDQDVINYVREKYNVLIGERSAED
                     VKSSIGSAVPQRKETKMSVQGRDLLTGLPRTVELSSIEVREAIMSSISQICEGVKDAI
                     EDTPPELLKDLLASGLRLAGGGALIKGIDTYLSQELNLPVKIVDDPLSAVARGTALML
                     DHIELLERIQKSWEELV"
     gene            complement(7238..7456)
                     /locus_tag="PKI16_01345"
     CDS             complement(7238..7456)
                     /locus_tag="PKI16_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HNR53108.1"
                     /translation="MSKICNICGKTTMAGQRIQHKHSVGWRYKAPKSKRIFEPNLRKV
                     DTEVDGAVVKASVCMKCYKKLRKDSLEK"
     gene            7522..8142
                     /locus_tag="PKI16_01350"
     CDS             7522..8142
                     /locus_tag="PKI16_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR53109.1"
                     /translation="MITTETLYWILFAIPSILIASTVHEYAHGLAAYKLGDPTAKAYG
                     RLTLNPLKHIDPIGALSMVLFRFGWSKPVPINEYNFEKRELGTAIVSFAGPLSNLILA
                     AIFGSINLIFHPDLGSIFGIFLFTFTLINISLAIFNLIPIPPLDGHKIVRAFLPKKIR
                     YYWEKMEKFSFLFILLLIIPFSPIGKFVLGGISSILARLLALFGFL"
     gene            8393..8469
                     /locus_tag="PKI16_01355"
     tRNA            8393..8469
                     /locus_tag="PKI16_01355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8427..8429,aa:Thr,seq:tgt)
     gene            8475..8557
                     /locus_tag="PKI16_01360"
     tRNA            8475..8557
                     /locus_tag="PKI16_01360"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8509..8511,aa:Tyr,seq:gta)
     gene            8635..9348
                     /locus_tag="PKI16_01365"
     CDS             8635..9348
                     /locus_tag="PKI16_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53110.1"
                     /translation="MSTLFPIIFAILSILGTLLVVGGVVYAIIYFTKKKKTEKFSLSV
                     DLLLKIYLYVISIATLAIAVIGGTMLLRAGSSYAFGIPFAYETYKPNELMDIEVKEPN
                     ANTPECYEGEKTQINGQTVCFDNDKIKQELINGGTIFFSMVILFTIHMIGIKMIEKKN
                     VTEWLRKTYTFLSLILYSLVGIVTIPIAIYQLSTHLLYRTEDITKYEAPATAIAILIL
                     VLPLWIYYLLKTTKLRNKN"
     gene            9370..9446
                     /locus_tag="PKI16_01370"
     tRNA            9370..9446
                     /locus_tag="PKI16_01370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9404..9406,aa:Thr,seq:ggt)
     gene            9545..9709
                     /gene="rpmG"
                     /locus_tag="PKI16_01375"
     CDS             9545..9709
                     /gene="rpmG"
                     /locus_tag="PKI16_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNR53111.1"
                     /translation="MAKVKRDIIALKCTVCNSKNYTDYKTKNIKEKIELKKYCSKCQK
                     HTSHKETKVK"
     gene            9730..9806
                     /locus_tag="PKI16_01380"
     tRNA            9730..9806
                     /locus_tag="PKI16_01380"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9765..9767,aa:Trp,seq:cca)
     gene            9821..9994
                     /gene="secE"
                     /locus_tag="PKI16_01385"
     CDS             9821..9994
                     /gene="secE"
                     /locus_tag="PKI16_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNR53112.1"
                     /translation="MKILKNILTELKKSKWPKGKELLVLSIYALVLCGIITAIILGLD
                     LVFYKAREWFLNL"
     gene            10005..10562
                     /gene="nusG"
                     /locus_tag="PKI16_01390"
     CDS             10005..10562
                     /gene="nusG"
                     /locus_tag="PKI16_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNR53113.1"
                     /translation="MAKEEVKTKENPNAKWYVLNIRTGYENSIQKELKQRIEATGMED
                     KIVDILIPVQKKIFVKKGKQDVKEEKIFPGYVLIKMELDNRTWDLVKNTEGVRGFVKT
                     DRYPRPLPESEVRAITKFMEVEQPSFQNSFSVGEAVKIVDGAFADFIGSVQDVDTEKG
                     KIKVLISFLGREAPVELDLTQVSKL"
     gene            10577..11002
                     /gene="rplK"
                     /locus_tag="PKI16_01395"
     CDS             10577..11002
                     /gene="rplK"
                     /locus_tag="PKI16_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNR53114.1"
                     /translation="MAKEIKKIIKITIPAGGATMAPPIGPTLAPYGINAQDFCAQFNE
                     KTRENNGILTPVILTIYEDRTFGFELKTPPTSELIRRELKLKAGSAKPNLEKVGKLSK
                     EQIKKIAEVKMPDLNTSNPEQAAKIIAGTAKQMGVEVQL"
     gene            11020..11709
                     /locus_tag="PKI16_01400"
     CDS             11020..11709
                     /locus_tag="PKI16_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNR53115.1"
                     /translation="MKRGKKYTKSTKGLDKSIEYTLTEGVKKAKALSYSSFTGTLEMH
                     VDIKIPKDRDSKSIKGAVSLPHSSVKKEVKVAVFCTPDKDVLASKAGADIVGLDNLIK
                     DIKAGKIAFDVAIATPDVMPKIAILGKELGPKGLMPNPKNGTVTNDLEGAIGEYKKGK
                     QTFACDDSGVIHLNVGKLDLEDEKLIENIHTVVLALEEIFGKSYEQIVDRMHIAPTMG
                     ASVKIAYSKTE"
     gene            11807..12145
                     /locus_tag="PKI16_01405"
     CDS             11807..12145
                     /locus_tag="PKI16_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53116.1"
                     /translation="MANEGSVIEFEGCKYIICKDYGDMALISSLALKQHFLLFLCPVV
                     HILPCTGKNFSRYVKLEKGELIFNGVHFPVNRYDMPSCNVKNLESNLHGKIITFSIVE
                     RTKELMLVKA"
     gene            12354..12731
                     /locus_tag="PKI16_01410"
     CDS             12354..12731
                     /locus_tag="PKI16_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53117.1"
                     /translation="MTTASVLAPCKTCDLKVCSGPEDCPYVIKEEIPFPSIKPAVHNQ
                     KYALKIPNDISPILKKNAIAFPQDGKISIFWDKKIKKVSIVILDNTKKKDNRQTVFHV
                     SGNENLKKRTLHFRGNVLIKIQS"
     gene            12919..13440
                     /gene="rplJ"
                     /locus_tag="PKI16_01415"
     CDS             12919..13440
                     /gene="rplJ"
                     /locus_tag="PKI16_01415"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNR53118.1"
                     /translation="MSKSRSQKTELLERYKEILKSKEGYFLINSDKADTLTTTGLKIQ
                     LKSTGANLAVVKNSIFKVALQETEQPLSTQEFDGPTAIVYFDEDPTAPAKLIKEAQKK
                     TQLFDARGGVYEGEFLTAEKVMQLAEIPSREILLGKLLGTMSAPVSGFMNALAGNVRG
                     LTMVLKGISEKGA"
     gene            13480..13884
                     /gene="rplL"
                     /locus_tag="PKI16_01420"
     CDS             13480..13884
                     /gene="rplL"
                     /locus_tag="PKI16_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNR53119.1"
                     /translation="MADENTKLPELSENSKKVLEIVEKMSVLELADLVKVMEDKFGVS
                     SAAPVAVAAAPAEPEEPEEEKTIFNIVLKEAGANKIGVIKIVREITELGLKEAKDLVD
                     AAPQTVKEGVKKEDAEAYKAKFEEVGATVELA"
     gene            complement(13947..14645)
                     /locus_tag="PKI16_01425"
     CDS             complement(13947..14645)
                     /locus_tag="PKI16_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53120.1"
                     /translation="MTEKKPLLIVLFTFLVVSLLASLGGLYYFFTKYRALGTVNQTIE
                     KEEYRDLVHDLLVQIDEMEPTLESSDGEDENLGEIREFDVDGILMKDFIELFKEKYGN
                     DMDNIPNFYYFDNSYLDGTAKYTLFSGITDPYGKEVNYANLLVTVVVDGYKISFTQGV
                     EGLGGRCEEEDNSCCDSIVESGGISFWSDGYGYQGYPEDGSGMTVDMENSIFDSICGN
                     KEIEKGIFSKLRKL"
     gene            14766..15434
                     /locus_tag="PKI16_01430"
     CDS             14766..15434
                     /locus_tag="PKI16_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HNR53121.1"
                     /translation="MDYTPFTKWVLDNGLSERTLELLVGIAIVATIVSLARYLFGSKS
                     FGIYAPVILAVAFTYTGLRYGLVITAVVITSSLLSYLILSKIRMHYISRMAINYCLII
                     MSLIIFFIIVHKYNLGLPNMSLIQPLAIISIIGLSDFFAKQQIKKGFKTSILVLGSTL
                     VIASIGWFIITREAISTFMINNLWLIPALIVINILIGQFSGLRFKDIVRFRTIIQDEE
                     NVKK"
     gene            15421..16881
                     /locus_tag="PKI16_01435"
     CDS             15421..16881
                     /locus_tag="PKI16_01435"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HNR53122.1"
                     /translation="MLRSKKIKEILGLNRRNQEYIRPLNSPSSKKIADNKILTKKVLS
                     KEGINTPEVYKLIRTKKQLQFLDFESLPNSFVIKPNQGTGGNGIVVLYGKKKGQTAWI
                     RPSGEIMSTKDITMHIENILEGRYSMGSRKDIAIIEERVKTDPFLKQYSYKGVPDIRI
                     IGYNQVPIMAMMRLPTKKSNGTANLHSGAICTGIDISSGITTYSMHMQDKSLISDSYE
                     MIDSTLDLKENKKLAGIQIPYWEQILEIAVKCQKASKLGYIGVDIALDAEKGPIVFEL
                     NARPGLGIQVANQAGLRWRLEKVKDIEIKSMKHGIRVAKNLFGGEVEESIEAISGRKV
                     VNINEKIFLYYKTPVSKKKNDPQNREAIRVFVDTGLITSRINKTLASRIGFFEAIKYF
                     YSLPIPDKFESFKDAQEYIDSNQTTIIGHPDILRVAKIVEEGTIKIRPVICVHTKIAS
                     EEKEIEMVVTEDTTYNVVIGRNELKGYLIDTSKTFT"
     gene            16883..17875
                     /locus_tag="PKI16_01440"
     CDS             16883..17875
                     /locus_tag="PKI16_01440"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53123.1"
                     /translation="MKKYEEYLKEAGNVHLAYYLEAADELGIEYEIVVRSLLARFTYQ
                     GKHWFIINTVTPLTPSPGTTIAKRKYLTNLVLSRADIDVPRQQALGSAIDAIKFFNKY
                     KNIVIKPSQQLGGIGITMLPETEEQVIEAYESAFQKSNAKGAVKVLGEEFIKGENYRF
                     LVVGDQVVGIVWRKAPTVIGNSKNTIRELIDIKNKERRMLVLKPIPIDTEVEIRLRNQ
                     ELTLDSIPEEGREVVLRYNCNLTTGGTTEECSSIVDPYYKQLAISAVKELGLEFGGVD
                     IIAQDITKQDKCGINEINYNPGLRLHYKVNKGQIVRVAIPIMEYIRDKYINSVK"
     gene            17878..19551
                     /locus_tag="PKI16_01445"
     CDS             17878..19551
                     /locus_tag="PKI16_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53124.1"
                     /translation="MVKVLQGLNLENKTSVIEINAIKTDNLEKLLQLIHSFHPIFLEE
                     YKYEENTLKIYSNLPFLWEELGKVLEQDLPYNKTLEQCLEIIKLRVSSMSTIPLLFAA
                     HKLGYETTPTMTKNESLTYTPSFNRHYTVGCGKGSEIIYSISSSKDSKIAKEIQRDKW
                     SSNLLIEKLGLPLPAWEIIDSFTEIEEIWKRFDKPVVIKPTGLTGGSGVCIGIKTIEE
                     AKEAYKYAKQVIDTKIRKDWQTKVMIQEQVSGEDYRLLVIDGHLEVVTKRIPAFVIGN
                     GKDTITKLIEDTNKDPRRDTTSPAHTLKPIEIDQPLRAYLKEQNLELDSIPDKDQKIP
                     VRKVASMSQGGITEDFTDKVGPEIKSIVESIASSIHAFALGVDVMCKDISKPLTKDNG
                     AILEINTMPEAYLNLFPVIGPDRGYVADTYIKKLLTNNKTKKIVVIGHSSYDIPTTLK
                     QKTLLSSYLRDEDTVGEYKDGEIRINSLVLNKGLTKRQGIEALKLNASLDAIIIHHRN
                     WEEVIKDGLGLNKIDLLMVESSLKENSEYMKIIKKYKRKGLISKIKIFQ"
     gene            19551..20495
                     /locus_tag="PKI16_01450"
     CDS             19551..20495
                     /locus_tag="PKI16_01450"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53125.1"
                     /translation="MKYLVIDKRPRIKKMEIPSASSRILEELKKKDIPADFVYNDEIE
                     ILFMDGSTQIKAKGIDVKEYTHIIFRGHALHNDKQYALKRYIIDYIEQHNSDNPEKKV
                     LIQNSKAIKKLPYYNKIAISLLCSQYNLPYFQTYYRTDGKYLEPRDMLKEYPLIIKEY
                     AGINRIEIIDGEEKIKKNVFKLEKDEDFEQDGLKDQDLSNFFLQEFSNTAEDYRIFVK
                     LGKVIGGWKRTASKGFMTVNKGIYEAYNQPNEQMREIAEKMANILEADFMAVDFMYMN
                     GKPCIQEISLHPGFKAYETKIKGEHINIAEAIIDAFSD"
     gene            complement(20492..21655)
                     /locus_tag="PKI16_01455"
     CDS             complement(20492..21655)
                     /locus_tag="PKI16_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1704 domain-containing protein"
                     /protein_id="HNR53126.1"
                     /translation="MNMFFRKAKKTMKVEEVTRLLGELKIPIALVFTPTNLNSEKKKF
                     FDSDTYEPQFQYRVVKNKNEEIFKDLASVKEISDVDPRISNFYIDLIDSKREASDLMH
                     AVGNNEAVTDISIARYGKPSAKLFRNSARVLRGRYGRYNLAKENKERDPEMLGYDSIV
                     SVFDVVFRELGLEDWSVNKSLNIAKNGVKVGVKRKEVLVDGNISKSKFQLRKTLIHEV
                     GTHALRSYYGLKSGFEAISKPNLASYLDVEEGLASWNENNMGLLPEKSLRNKAGLTWA
                     IYVGEELSFRQLYNVLLGNFSKLGAFDIAYRVKRGLSDTSYPGIYAKDVVYFRGFRKV
                     MKKLEEDPTLYQKLYSGKISFKQCEWVEDGLIPKASKIPSKNDWIDIFRKAGI"
     gene            complement(21690..22709)
                     /locus_tag="PKI16_01460"
     CDS             complement(21690..22709)
                     /locus_tag="PKI16_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNR53127.1"
                     /translation="MFGKKLGIDLGTANSVVFVQGEGIVFVEPTVVAIDVNKFTVVAV
                     GNEAKEMIGKTPENIVAKRPLRNGVIADSRVTEALLRFFFDKALGKSRFFKPDVVISV
                     PAGITSVEERAVLKAANAVGAKEITLMPEPLLAALGAELPIHTSSGNMIINMGGGTTE
                     VAVMSLDGIVEYESLRVAGDAINEAIIMYMRKKKGLLIGEQTAERVKIKIGSALEVKD
                     PKEMEVRGRDVGAGMPKSIVIDSNDIAKAVERPLRSIIKSIQSVLEKTPPELSADIID
                     RGMVMSGGTAMLRNLDRLFTKATGVPSHVADDPAFCVAKGTGIALELLAAGDRKFSFN
                     REFRR"
     gene            complement(22729..23739)
                     /gene="trpS"
                     /locus_tag="PKI16_01465"
     CDS             complement(22729..23739)
                     /gene="trpS"
                     /locus_tag="PKI16_01465"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNR53128.1"
                     /translation="MNDKKRILTGDRPTGKLHIGHLMGSLRNRVRLQDEYDEYVIVAN
                     VQALTDNFDNPKKVKENIQELLCDYYAAGIDFNKATVFIQSEVPAIHEIFMYISNFAT
                     VQQVQHNPTIKTEIAQKGLENSTPLGFFIYPVHQAADIFCVNADLVPVGKDQAPIIED
                     SRVLARKFNNTYGVKLLHEPEALFGTEVNVPGIDGNSKMGKSLNNCIFLSDSEEELRK
                     KVFQVYTDPARVHATDPGKIEGNVAFMYHDLFNSNKEEVEEMKDLYRKGEIKDVEVKE
                     RLFEVMNEYLKPIRERRVEAEGMKDELLAKALDGSKKVQVIAKDIVEQMKEAMELGFD
                     NR"
     gene            23788..25314
                     /locus_tag="PKI16_01470"
     CDS             23788..25314
                     /locus_tag="PKI16_01470"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNR53129.1"
                     /translation="MSKEHIILKNKILPALLPILTSIIILLGATIIYLYSSGYRIDIL
                     KKEINITGVITVQSDPSATDMFVNGEKIGRAPRSHVLKVGEYDISLKKEGYQDWNKKV
                     KVLEGKSTPIFPYLVYDKIISKEKWKSEGLIEKLWTSDTRNDLLFLQKDSNTSFSLWK
                     YAINPLLWDFSTNPSKILTLDTNKISLSISPDGQQALLVMTDPKSPLYYLLNTQQPNT
                     LQGSNQLPFGNYSKYTMSWSKDGKYILLDSSKELLSYNLSNQTFTTILKKDAETKYVW
                     DTDTQGFFYLIIEIQNDELEDIYTYTLTQKSLAGNNNKTIIDRIYFQQTDEYIKEYRK
                     SATTLQEFSNSPESIQTAGEVIDLDVNVNGKGMYISTSLASYWYFMDTSKFMTISPYP
                     TEIISYSPDIYKLGVKDTTGYKIFTFYKEEGDHTIDLGAKSLNNINTNFFWLSNSSYI
                     YLKENNSIYIADIDGENKYQLLESENVLEYIVNNSREILVTFEKDSTGKLVIIENKFH
                     "
     gene            complement(25319..25392)
                     /locus_tag="PKI16_01475"
     tRNA            complement(25319..25392)
                     /locus_tag="PKI16_01475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25356..25358),aa:Arg,seq:cct)
     gene            25480..28329
                     /gene="uvrA"
                     /locus_tag="PKI16_01480"
     CDS             25480..28329
                     /gene="uvrA"
                     /locus_tag="PKI16_01480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNR53130.1"
                     /translation="MKQEYIKIKGARENNLKNISVDIPKNKLVVFTGVSGSGKSTLAF
                     DTLYAEGQRRYVESLSSYARQFLGVMKKPDVDSITGLSPAISIDQKTTGTNPRSTVGT
                     ITEIYNHLRLLYGHIGQPHCPKCGSPIQSQTIQQITNKVIKQISNTSEVTQIVSPIIK
                     NRKGEFTALLNKLLSKGFLRVIIDGNTYLLDDIEDIKLEENNKHTIDLVIDRIDSTKL
                     DKKRLTDAIELAITQSNGEVKVLLKDKELFFSENNTCPKCEISYPKITPASFSFNAPE
                     GACPKCAGLGVLKEIDINLIYNPILTILEGGIFPWSNRTSKDSWTLRILREIAKEHGF
                     NLTTPIGRYPKEIFDLIFYGNGTKDAYTIEYTNRFGRTRLYEAKYEGVIPELERRYRE
                     TSSDYARFETEKYMRENICPVCEGKRLKPYSLAVTIGGKNIDEITNMPTKDVLTFFKT
                     LELKGSKKDIAEPIIKEINQRLNFLENVGLNYLSLSRKANTLSGGESQRIRLASQIGT
                     GLSGVLYVLDEPSIGLHQRDISKLITSLKDLRDGGNSIIVVEHDEEMTKSADWILDIG
                     PFAGKHGGDIVAKGTLDDIKHSDSSTAKYLNKTLSVGQDAPLLQVGARGNEISLRGIK
                     THNLKDLDLTIPLNQLICITGVSGSGKSSLIADTLYPVLANRISHSKLQEGKYDSIEG
                     LEHINQIINIDQSPIGRTPRSNPATYTGVFTKIREIFAQTNEAQARGYKAGRFSFNAK
                     GGRCEKCRGDGVLKIEMQFLPDMYITCDECNGSRYNDEALQIDFKGKNISDILKMTVE
                     EGLEFFKNIPSIHRKLSVLNEVGLGYIELGQSALTLSGGESQRVKLSKELSKMSRGHT
                     LYILDEPTTGLHYHDVDKLLVILRNLVKKGHTVIIVEHNMDIVRHSDWIIDLGPEGGD
                     QGGYIIAQGPVSDIIKSKTSWTGKYLKKYISETSG"
     gene            complement(28524..29996)
                     /gene="proS"
                     /locus_tag="PKI16_01485"
     CDS             complement(28524..29996)
                     /gene="proS"
                     /locus_tag="PKI16_01485"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNR53131.1"
                     /translation="MSNKGITKREQDYSQWYLDIIERAELAENSSVRGCMVIKPYGYA
                     IWELIQKELDKRIKESGHKNAYFPIFIPKSFFSKEASHVKGFAKECAIVTHYRLKESE
                     DGKGVVVDESSKLKEELIVRPTSETIMYDTFSRWISSWRDLPLKINQWANIVRWERRT
                     RPFLRTAEFLWQEGHTVYPTDEESKKEVAEMLQMYSDFAREYLAMETISGKKSESEKF
                     AGAVDTYGIEAMMQDGKALQFGTSHDLGQNFAKAFGIMFTDKDGEKKYPYQNSWGVST
                     RMMGAMIMTHSDDNGLVLPPTISPVKVVIIPIFNEDEKEKVLSYCKDIKESLEDIDGV
                     EIDDREYVSAGFKFNEWEKKGVPVRIEVGPKDIQNSQAVLVRRDTGEKENVGIEKIED
                     TVETMLKSIQDSLLSKSKELLVSKTHRVGSYEELKAVLSSEKGFVLAHWCGNSECEEK
                     IKSETKATTRCLTKEGIGSSGKCIICGKESKEEWVFSVSY"
     gene            complement(30028..>30662)
                     /locus_tag="PKI16_01490"
     CDS             complement(30028..>30662)
                     /locus_tag="PKI16_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HNR53132.1"
                     /translation="DRIKAQGGRWMMLDVVVTGFAAMHTMRLKNPGLAIHAHRAMHGL
                     FDRDSGSGVYDKGYIADFSMSMVAIAKIMRLLGVDSIHGGAPKTKMENYGEPKLIRDA
                     LQLDVTPAASMTLGQNWYGMKPVWHVASGGLHPGTLGESIKQLGEDIIIQCGGGVLGH
                     PWGIEAGVEAVVQAKDIALGRGNIDDWILQNPDSALAKAAQHWGFEGKIV"
BASE COUNT        10177 a         6144 c         4993 g         9348 t
ORIGIN      
        1 acttctcaca agcccagaac ttaccatatt tactattctt gagtagcaat ttactaccac
       61 actttggaca tttctctggt ttggcatatt ttttgaaatc caaactctct tctccaccag
      121 aaagatcttt catcccctta cactctggga atttggaaca tgttaagaat ttaccatacc
      181 ttccaaccct aactagcatt tcagaaccac ataccggaca cttctcttta gatttagaaa
      241 gtatgacaat atcttcctta tttacatcct ttagtgcttt ctctatctct gctacaagtg
      301 gcttgtattg ggcttctatt actggaatat attcactctt accattggca atatcatcta
      361 attcatcctc aactttagcc gtatatttgt aatctactaa tcttttaaaa ttgttcctta
      421 gaagatttgt aacaaccttt cctacatctg taggcaaaag tgctttctct tcttttgtga
      481 cataccctct actttgtatt gtagagataa tggttgcata tgtagaaggt ctaccaatgc
      541 ctaactcttc aagctttttg accagtgcag cctcggtgta tctagctggt ggttgtgtaa
      601 atttctgctc ttttacaaac tgatcccatt taagtatatc tccctttgct atctttgaga
      661 cttcctgaag atcactgtct ccttttccac caccaagtgc tcttctgaaa ccgtcaaaaa
      721 gggttttctc tccacctaga gagaatgtat acaaaggttt tttcttatct ttgctttcta
      781 tatgtattgt tagtgtttct gcctctttct ctttcatttg agatgatatt gctcgtctcc
      841 agatgaggtc gtagagtttg gcttctgcag gaccaaattt agaatcaatt gctgcttttt
      901 caaggctaaa atctgttggt cttatagcct cgtgtgcttc ttgtgcgttt ttacttttag
      961 ttttgtagta atttgcttgc tctggaagat attccacacc atatttattt tttaccaatt
     1021 cccttattcc atctatggcc tttgagctta aatttacaga atctgttctc atatatgtga
     1081 ttacacctgc ttgatacagt acttgtgcta tacccatcgt tcttttagat gaatatccca
     1141 gaatattgtt tgcagattgt tggagtgtag aggttgtaaa tggtggatat gcatgtcttt
     1201 taatattctt tttgagcaca tctactactt cgtaacttcc ctttcctagg aattcttcaa
     1261 tgtcttttac ctcttctgct ttaacaggaa gatatttttt accatctttt tttactaatt
     1321 ttgctcttag agtatctcca cttgggcttg ttgtatctac aaaaaactcc cagtactcct
     1381 cagagtcaaa agcatctcgc tcctcttctc tttctacaac caatctcaga gctacagatt
     1441 gaactctacc cgcagaaaga ccgtaccaaa ttttcttcca aactaattca gagagtttgt
     1501 aaccaaccaa tctatctaga actcttctag ctttttgtgc ttctacaagg ttgatattta
     1561 tatctgatgg cttacttact gcctctaata tggcatcttt tgttatctca tggaatgtga
     1621 ctcttttagt atttttagct ttcaaaactg aatctacatg ccaagctatt gcctctcctt
     1681 cacgatcagg gtccattgca agatatacat ggccatcttt tggaacagcc tttttcaaat
     1741 cctgaattac cttaccctta ccatatatcg tttcgaaaag aggtttgtac ccatcttcaa
     1801 catctactgc aagcttgctc tttggaagat ctatgatgtg gcctattgta gccataacct
     1861 taaaatcatc tcccagatat ttatttattg tcttggcttt tgcgggagac tctactatta
     1921 gaaggttttt atatttttta gttgattttg tcatttttat tcgcttccta aaaatacagt
     1981 atatattcta ccattgtcaa acatgtttat atttcttata catctacagg gcctatacct
     2041 gctctgtaca aagattatat tcctattcgt atttgttctc catctattcc cagtatatca
     2101 aggaaatctt ctacaccagt tattatatct gcaccctgtt ttaacagact attgcacccc
     2161 tttgaactct cccttgtaat atcacctggg acaacaaaaa catctcttcc atattcaaga
     2221 gcaagattcg cagtgagcaa agatccacta tccaaacctc cctcgatgac aattgttgtg
     2281 tctgacatac ctgccaatat tctatttctt attggaaaca tctcttttct tgtttctgtt
     2341 ccctcaggga attcggccac tacagtacct tttttacata tttcctcata tatctccctg
     2401 ttttgtgctg gaaaaccttt gtcaataccg cctgcaatca ctgcgattgt agcaagccct
     2461 ttttccaagc acattttatg cacctgacta tctataccaa ttgctaaacc actaacgaaa
     2521 gccacattta attccttggg aacagacgaa agcaggtttc ttactaccca ccctccatat
     2581 gtactcatag ctctagttcc aacaattgta attagtcttc ttgagagtat ctctttgttt
     2641 cctttgcaat acaagacatt tggatatcta tatatattcc tgaggttgcc gggatactct
     2701 ttgtcagcag cttttatgat gtacataaca aagattatgt accaatattg ttaaaaatat
     2761 cttaaatctt ccctgcttta accatccaca taatcatgct ttgcataatt gctacagcat
     2821 tggagctatg cccaccatct tcaagaaata ctgaaaaaga gtacttaggt ttgttgtatg
     2881 ggaagaaacc tccaacccat gcatgagtat tctcgtagat tcctttcgaa tttacctttc
     2941 cgaactctgc agtacctgtt ttagcagcta caactactcc gacattggcc aactgaccta
     3001 cagatctctt tggaccgttt acagcagacc acatcccctc cttagtgata ttaaaggcct
     3061 ccttggacac catgttttct tctagaaggt caaaggttag agtttctgtc tctccttgct
     3121 catctctgaa agatttcgca acatgtggag tatgtaattt tccttctgta gcaagggctg
     3181 ccatccagtt ggacatttga attggtgtta ccaaagttat tccctgccca ataactgagt
     3241 tacaggaatc tccctctgga taccacacag actcaagcca tggacttgtg gtctctgcca
     3301 agagcttttt gtttgctgga gaaggaagtc ttcctggtgc ttctcccggt acatctatac
     3361 ctgtatattt tccaattcca aacttctcta agtaaggatc taacttgtta atatcccagt
     3421 ttctaattaa ttcacaaaag aaaatatttg aggactgagc aataccacca acaacattca
     3481 aagctccata tgcatggttt tgaaactctt gaaaaggagc tccattggag aacttgtacc
     3541 catatctgct gacatatatt gtgtttttag ttatggcacc agcatctaat gctgcgggtg
     3601 cgacaagact tttgaaggta gagcctggtg gcatctgaac agagatagcc ctatttatga
     3661 gaggcaggtt tttgttattt agtaatttag agtaattctt tgttgagatg ccaccaacaa
     3721 aaagattgtt gtcatagcta ggatatgtga ccatagctat aatctctcct gtttcaacat
     3781 cctctatgat acctgcacca cctacagcgc cagcctttct aacaccactc tttaggaaat
     3841 catagaattt tctctgaata tctatatcta ttgttaaata caggctatct ccagatactg
     3901 cttcttttat gacatattct tggttagaaa cagatcgccc aagagcatct acttctattg
     3961 ctatttcacc attttctcct ctaagttgct catcgtacca tctttctaca ccacttttac
     4021 caattgtatc tgtcaaagca agagattcaa aagattttag atcttcacct gttacatcac
     4081 cagtatatcc aatgatatgc gagattatat ctttgtatgg ataatatctt tggctatcat
     4141 tatctatata tataccaatc aatgattcac tttttgactt tatctctatc gctttttcat
     4201 tacttatgtt ctctgcaatc aatatatctg taaaatatgg actctgcttg tgtacagaat
     4261 aaattttttc aagaatgcta ccatactctc cacctgcaaa agtcttcttc cacatgtcac
     4321 ccagcatacc cccgagggta ttagcggtgt tctctaacat ctcagctttt atcgctcctt
     4381 tatcatccag gtaatcctcc atcttgatat acaaattcat agaaggtatg ttctctgcaa
     4441 gtttcttacc atttcgatca aaaattgcac cacgataagc tgtttctgtc tttactttaa
     4501 tctggttact gttagatttg tccagcattt ctttcccttt gactatctgc aatgtcgtta
     4561 ggttccctag cagaacgaca aaaaggataa agaatataaa gaaaacaaaa atgtaattcc
     4621 acggactacc cttctccaca gcagtaggtt tggagaaata tttctttact gaaaagctaa
     4681 aaggctctga gcgcccctta gaagcctgct tttttcttct gggtatgcta ttcttcattt
     4741 ttttagacag ttttaattcc attttttgct tatttttata ttattctcct tacctctagt
     4801 aaaacttaac aatatgtcca ttatgtagag gacgataacc agtaaaacag acttaatgcc
     4861 aagaatcaaa accatatgcc acgttaaaat accaaaactg ccatttagga aaagaggggg
     4921 tagaactaaa attaaaacga agtaaagcgc aattgtaatc aatttgattc catacccaac
     4981 cactccttcg ttgatattga atatgaaaga gctaccaaat agtagtaata aagggatacc
     5041 aactaaaagg atatctattc caagtggata gtggaatact acgtcagaaa ttaatgcata
     5101 tattactaaa atccctgaca accacttgat atctaacctc ccaagaagca aaagaattaa
     5161 tacaaagaaa atcctaattc caaacgcact aagaaggaag ccttcaataa aaagcgctcc
     5221 tattatccca attattatat acaagacttt tagcatttat ctttctctta atttattctt
     5281 acaaaaacct tacttaaagt atcataatct acaattgtag aaacaaaaca tgttcttgaa
     5341 atactggcag gattacttga aagaccaaca acataaccca aaaccaacat atccccaacc
     5401 gaagaatcgt ttacaaagat tatgtctcca tcatttatat ctgaattcat tgttatattc
     5461 tcaaccttta taccctctgc ggagccattc attacaccac gacttattac tttgtttttt
     5521 agatatccgt ctaatgtcct actctcgtcg tactttaaaa tactaacacc aagatatgaa
     5581 ttattacttg ttggaagttt aatacgagag cctagttcat ccacatactc tactacacca
     5641 acaaagaaat tcccaactac tgccacatct cctacttgaa tgccggattt tgttccttca
     5701 tctatgcgaa gatagtcgat accgtcgctt ttcaaaacat ttcccattac atactctctc
     5761 cctttgttct ttaggcttaa ttgttttttc aaaagttcat tttcagccaa aatctctaag
     5821 taagaggcct ctcctatttc attttctgct atttgaatct ttaatctttc attttcctct
     5881 actatatctc ttaaactaac aaagtttttg aagtaggaag agattccttt gcccaaaccc
     5941 tctcctgtaa tgaaaattgg ctccatactg taggagattg gatatctaat gaagttagtg
     6001 acattagctt tatctaaaga aagaagaatt ccaccaaata aaataacaat tatggtaaaa
     6061 attagctctt ttttaaaatt accttgagca ttcattcctt aagagataat gttaaacaag
     6121 ctcttcccaa gatttctgta ttctctccaa aagctcgata tggtcaagca ttaatgccgt
     6181 tcccctagca acagcagaga gtggatcatc tacaattttg actggaagat ttaattcctg
     6241 agaaagatat gtgtcaattc ctttaattaa agcacctcca cctgcaagac gaagccctga
     6301 tgctagtaaa tctttcaaaa gttctggtgg agtatcctca attgcatctt ttactccttc
     6361 acatatttgg gaaatagaag acatgattgc ttcccttact tcaatactgc tgagctctac
     6421 tgttcttggt agtccagtta gaagatctct tccctgtaca ctcatcttcg tttctttcct
     6481 ttgtggaact gcacttccaa tggaactctt tacatcttct gcacttctct ctcctattaa
     6541 tacattgtat ttctctctta catagttgat aacatcctga tccatctcat cacctgctat
     6601 tctcattgta ttgtctacaa caatatctcc aagagagaaa acagcaatat ctgaggtacc
     6661 accaccaata tctacaatca tacttcctga ggcatcttct actggaagtc cagctccaat
     6721 agcagctgcc atggcttctt ccactatgaa tacttccctt gcacctgctg tttttgcagc
     6781 atcaagtacc gcttgccttt cgacttcggt tacagaagta ggaacaccaa taatgactcg
     6841 tggccttgga agtttaaaac ttttaccaaa atatccatgt gctctgtgta tgaaatgatg
     6901 aatcatggcc tgtgtagtat caaaatccga gattactcca tctcgcagag gtcttacagc
     6961 tgagatattg gcaggagttc taccaatcat ctgtctcgca tcttttccaa cagcaagaac
     7021 cgtctttgtt cttttatcta tcgcaacaac ggagggttca gatacaacaa ttccatgatt
     7081 cctcatgtac actatggtat ttgcagttcc taagtctatt cctaaatcat aggtaaagaa
     7141 ctctagtatc gaatttatca atttcatagc acaattctat cacttaaaag gatacaaaac
     7201 gagtatctac tgtattaaat ctgaatattg gtgaaattta cttctctaaa gaatcttttc
     7261 ttagcttttt gtaacacttc atacaaacag aggcttttac tactgcgcca tcgacctcgg
     7321 tatctacttt tcttagatta ggttcgaata ttctttttga ctttggtgct ttgtatctcc
     7381 agcccacaga atgtttgtgt tggatacgtt gaccagccat tgttgttttt ccgcaaatgt
     7441 tgcaaatctt tgacataacg gtggtattat attctaatct ttggcatatt gtcaaagatt
     7501 tagaactgat ataatagaat catgataaca acagaaacat tgtactggat actattcgct
     7561 atcccctcca tattaatagc ttctactgtg catgaatatg ctcacggatt agcagcttac
     7621 aaactgggag atcctactgc aaaagcatac ggaagactta cacttaaccc tcttaaacac
     7681 attgatccca taggagcact ctccatggtt ctttttagat ttggatggag taagcccgta
     7741 ccaataaatg aatataattt cgagaaaaga gagcttggaa ctgcaatagt ttcttttgct
     7801 ggacctcttt caaatctcat tctagcagca atttttggct ctattaatct aattttccac
     7861 ccagaccttg gaagtatttt cgggatattt ctttttacct ttacactaat aaacatatcc
     7921 ttagctattt ttaatctaat ccctatccca ccattagatg ggcataagat tgtaagagca
     7981 tttcttccaa agaaaataag atactactgg gaaaagatgg aaaaattctc atttctgttc
     8041 attttgctcc taatcatacc tttctcccct ataggcaaat ttgttctagg agggatttct
     8101 agtatcctag cacgattatt agcccttttt gggttcctct agcgacccca ttacaggccc
     8161 ttttttattt gttcctagca cataaatatc gtaaaatgta tgtgccctag taggaataaa
     8221 aggattcata gctctatatt caagagctcg ggattccaac tatcgcttag ctccgtggag
     8281 ttaagttgag gaaacccaac tgtattactg agctctatga ctccatctta ctggggcgtt
     8341 tttcccttgt atattgacta taattcccat atatattaaa cttctctcag ttgccgaagt
     8401 agctcagttg gtcagagcaa ctgttttgta aacagtaggt cgagagttcg aatctctcct
     8461 tcggctccag gtatgcagag gtggcggagc ggtcaaacgc attggactgt aaatccaact
     8521 ccttcgggat acacaggttc aaatcctgtc ctctgcatat gcctaaatct cctccttgct
     8581 ttaatgcaaa taatgtttaa aatgtatgta tatataaatt agatatattt ttacatgtct
     8641 acattattcc ctattatttt tgctattctc tccatactag gtactttact agttgttgga
     8701 ggtgttgttt acgcaataat atatttcact aaaaagaaaa agaccgaaaa attctccctt
     8761 tctgtagatt tacttctaaa aatttatctt tatgttatat ctatagcaac attagctatc
     8821 gctgttatag gagggacgat gcttttaagg gcaggctctt cctacgcttt tggtataccc
     8881 tttgcctatg agacatacaa accaaacgaa ctaatggata ttgaggttaa agaaccaaac
     8941 gccaatactc cagaatgtta cgagggagag aaaacacaga ttaatggaca gactgtttgt
     9001 tttgacaatg acaaaattaa gcaagaatta ataaatggag ggactatttt cttctcaatg
     9061 gtaattcttt tcaccataca tatgattggg attaagatga tagaaaagaa gaatgttaca
     9121 gagtggttaa gaaaaactta taccttttta tctttaattt tgtatagctt ggtaggtata
     9181 gtaacaatac ctatagcaat atatcaacta tctactcacc tactctacag aaccgaagat
     9241 attactaaat atgaagctcc agcaactgcc attgcaatac tgatacttgt attaccactt
     9301 tggatctatt atttattaaa aacaactaag ctacgaaata agaattagat attgtataat
     9361 tgccctgttg ctggattagc tcagttggtt agagcacatt catggtaaga atgaggtcct
     9421 gagttcgact ctcagatcca gctccatctt tattattcaa ctatttgacc ttacccataa
     9481 tttcccatat aatacgcagt atatttccca atatagacat ttttaatttt gatttccgag
     9541 ggatatggca aaagtaaaaa gagatatcat agctttgaaa tgtacagtat gtaattcaaa
     9601 aaactataca gattacaaaa ctaagaatat taaggaaaaa attgaattaa aaaagtattg
     9661 ctctaagtgc cagaagcaca caagccataa agaaactaag gttaaataat agctctttta
     9721 attaactagg ggcccatagt ttcaattggt taaaaccgcg gtctccaaaa ccgctattcc
     9781 ctgttcgaat cagggtgggc ctgccaaaat atatacaaaa atgaaaatat taaagaatat
     9841 attaacagaa ctaaagaaaa gtaagtggcc aaaaggcaaa gaattgctgg ttctttcaat
     9901 atatgctcta gtattatgtg gtataatcac cgctattata ttaggtctag accttgtctt
     9961 ctacaaggct agagagtggt tccttaatct ttaattaaga aaatatggct aaagaagaag
    10021 ttaaaacaaa agagaatcca aatgcaaagt ggtatgttct aaatattagg actggatacg
    10081 agaatagtat tcaaaaagaa cttaagcaga ggattgaagc tacaggtatg gaagacaaga
    10141 ttgtagatat cctaatccct gtacagaaga aaatctttgt taaaaaaggt aagcaggatg
    10201 taaaagagga gaagatattc cctggatatg tcctcataaa aatggaactg gacaacagga
    10261 catgggattt ggtcaaaaac acagagggtg taagaggatt tgttaaaaca gatagatacc
    10321 caagacctct accagagtca gaagtaagag caattacaaa attcatggaa gtagaacaac
    10381 catcattcca gaattcattc agcgttggag aagcagtaaa gattgtggat ggtgcattcg
    10441 cagatttcat tggaagtgtt caggatgtag atactgaaaa gggtaagata aaagtcctca
    10501 tatctttcct tggaagagaa gctcctgttg aattagacct aacacaagta agtaaacttt
    10561 agttaaatat atacatatgg caaaagagat aaaaaagatc ataaagataa ctatacctgc
    10621 aggtggtgca acaatggctc ctccaatagg accaacattg gctccttacg gtattaacgc
    10681 gcaggatttc tgtgctcaat ttaatgagaa aactagagag aacaacggta ttcttactcc
    10741 agtaatattg acaatttacg aggatagaac ctttggtttt gagcttaaga cacctcctac
    10801 atcagaatta atcagaagag aactaaagct taaagctggt tctgcaaaac caaatcttga
    10861 gaaggttggt aagctttcta aagaacagat taagaagatt gcagaggtaa aaatgccaga
    10921 tttaaatact tcaaacccag aacaagcagc aaaaataatt gctggtactg caaaacagat
    10981 gggtgtagag gtacaacttt aattatatat atcatcgcta tgaaaagagg aaagaaatat
    11041 acaaaaagta caaaaggttt agataagagt attgaatata cattaacaga aggtgtaaag
    11101 aaagctaagg cactctccta ttcttctttt acagggacat tagagatgca tgtagatatt
    11161 aaaattccaa aagatagaga ttctaaatcc atcaaaggtg cagtttctct tccacattct
    11221 tctgtaaaaa aggaagtaaa ggttgcagta ttctgtactc cagacaaaga tgttctagct
    11281 tcaaaggctg gtgcagatat tgttggctta gataacttaa ttaaagatat taaagcaggt
    11341 aagattgcgt ttgatgtagc aattgctact ccagatgtaa tgcccaagat tgcaatttta
    11401 ggtaaagagc ttggtcctaa gggattaatg ccaaatccta agaatggaac agttacaaat
    11461 gacttagaag gagcaattgg tgaatacaaa aaagggaaac agacattcgc atgtgacgat
    11521 tcaggagtta tacatcttaa tgttggtaag ctagatttag aagatgagaa attaattgag
    11581 aatatccaca cagtagtatt agctttagag gagatatttg gtaaatcata tgaacagatt
    11641 gtagatagga tgcatattgc tccgacaatg ggtgcaagtg ttaagatagc ctacagcaaa
    11701 acagagtaat tctcttttgt cattaccaca tcctttacaa tttctatcct ataggataga
    11761 atatatatgt ctttggctca ttatgaaaat taaagaggag taaagtatgg caaacgaagg
    11821 ttctgtaata gaattcgaag gctgtaaata cattatttgt aaagactatg gagatatggc
    11881 tcttatatct tcattggctc tcaagcagca tttccttttg ttcctatgcc ctgttgtgca
    11941 catattacct tgcacgggta agaacttttc taggtacgta aaactagaga aaggagagct
    12001 catcttcaat ggagttcatt ttccagtaaa caggtatgat atgccttcct gtaatgtaaa
    12061 gaatttggaa agcaatctcc atggaaaaat tataacgttc tctattgttg agaggacaaa
    12121 agagcttatg ttagtgaaag cgtagcaata catcgcaaca aaccaaagac agagagcgag
    12181 ctaatagtag cttgataggc gacttaaggg ccaatcaggc ttttatttct ataaatcatc
    12241 tattagctcc tctcagcacc atccaatatt acatatttac aaactatctc ccatagtata
    12301 gaatatctag gcaacaaaca tattttgttg ttctttcaaa aaaggagaat ggtatgacaa
    12361 cagcaagtgt attagcacct tgcaagacct gtgatttaaa ggtctgttct ggaccagagg
    12421 attgccctta cgtaattaaa gaagagattc cttttccatc aattaaacca gcggtccaca
    12481 atcagaaata tgcattgaaa ataccaaacg atatatctcc tatcctcaag aagaacgcta
    12541 ttgcatttcc tcaggacggg aagatatcca tcttctggga caaaaagata aagaaggtct
    12601 ctatcgttat cttggataac accaagaaaa aggataatcg tcaaacagtt ttccatgtct
    12661 ctggaaatga aaatctcaaa aagagaaccc ttcatttccg tggaaatgtc ctgattaaga
    12721 tacaaagtta gtttgttaaa cagagagaga agagtgcttc aaagcatttt tcaaaaatac
    12781 tcttctctct cctgcaattc ccttgaccat cccctatcct actgatataa tccacctagt
    12841 atttaaccag agaaacaagg tgtaaaaata aatccttgcg aggttaaaga tatacattca
    12901 attaaaaaat atatacctat gtctaaatct cgttcacaga agacagagct attagagaga
    12961 tacaaagaaa tactaaagag caaagaagga tatttcttaa tcaactctga caaagcagat
    13021 acacttacta caacaggatt aaaaatccag ttgaaaagta caggtgctaa ccttgctgta
    13081 gttaaaaaca gtatctttaa ggtagcccta caagaaacag aacagccatt aagcacacaa
    13141 gagtttgatg gaccaacagc aattgtttac tttgatgaag atcctacagc tcctgcaaaa
    13201 ttaatcaaag aagcacagaa aaagacacag ctctttgatg caagaggtgg agtatatgag
    13261 ggggagttct taacagcaga aaaggttatg cagttagcag agataccttc aagagaaata
    13321 ttacttggaa aattacttgg aactatgtcg gcaccagtat ctggatttat gaatgcgcta
    13381 gcagggaatg taagaggatt aactatggtt ctaaagggca tctccgaaaa aggtgcttaa
    13441 ccgaacatta ttttgtattt aatttaaaca tatataacca tggcagacga aaatacaaag
    13501 cttccagagc tatccgagaa ctctaagaaa gtgctagaga tagtagagaa gatgagtgta
    13561 ttagaacttg cagaccttgt taaggtcatg gaggacaaat ttggagtaag ctcagcagct
    13621 cctgttgcag tagcagcagc cccagctgaa ccagaagaac cagaagaaga gaaaacaatc
    13681 ttcaatatcg tccttaaaga agcaggtgct aacaagattg gagttatcaa gatagtaaga
    13741 gagataactg agcttggact aaaagaagct aaagatcttg tagatgcagc tcctcaaaca
    13801 gtaaaagagg gcgttaagaa agaggatgca gaagcataca aggctaaatt cgaagaggta
    13861 ggtgctacag tagaattagc ataattatat atttaagaat atatattaga agagccacga
    13921 aagtggctct tttttattgg caaatattac aatttcctta actttgaaaa aatgcctttt
    13981 tcaatttctt tattaccaca tatggaatcg aaaatggaat tctccatatc aacagtcatg
    14041 cccgatccat cttctggata accttgatag ccatagccat cactccagaa acttattcca
    14101 ccactttcaa cgatagaatc gcaacaagaa ttatcttctt cctcacatct tccacctaag
    14161 ccctcaactc cttgtgtgaa tgatattttg tatccatcaa ctactacagt tactaataaa
    14221 ttcgcataat tgacctcttt tccatatgga tctgtaattc ccgaaaaaag tgtatactta
    14281 gccgttccat ctaaatatga gttatcaaag taataaaagt tagggatatt atccatatca
    14341 tttccatatt tttctttgaa taactcaatg aaatctttca ttaagatacc atctacatca
    14401 aattctctaa tctctccgag gttttcatct tctccatctg atgattcaag agtgggttcc
    14461 atctcatcaa tttgcactaa caaatcatga accaaatctc tatactcttc tttctcaata
    14521 gtttggttta ccgttcctaa tgctcgatac ttggtaaaga agtaataaag tcccccgaga
    14581 cttgccagca atgatactac cagaaatgtg aagagaacta ttaaaagggg ctttttctct
    14641 gtcatttaat aaatttgatg aatttaatac aaattaagaa tacattaact cccatcgaca
    14701 aagcaatatc caaagaagta taattatact gaatctgcca atatattaat ttatcaatat
    14761 atctaatgga ctacacacct ttcactaaat gggtattgga caatggactt tcagaaagaa
    14821 cattggaatt gttagttgga atagccattg tagctacaat tgtaagctta gcaagatacc
    14881 tttttggatc taaatccttt ggaatatatg caccagtaat actggctgta gcatttacat
    14941 atactggatt gcgatatgga ttagtaataa ctgctgttgt tataacatca tccctactct
    15001 cttacctcat actaagtaaa attaggatgc actacatttc aaggatggca ataaattact
    15061 gcctaataat aatgtcctta atcattttct ttataatcgt acataaatac aatcttggat
    15121 taccaaatat gtccttaata caaccattag caattatctc cattattgga ctgtcagatt
    15181 tctttgccaa acagcaaatt aaaaaaggtt ttaaaacctc catactagta cttggaagta
    15241 cgctagtaat agcttccatc ggttggttta tcataactcg agaagctata tccaccttca
    15301 tgattaacaa tctctggtta atcccagcct taattgtcat aaatatatta atcggacaat
    15361 tctctggcct tagatttaaa gacattgtga gatttagaac cattatccaa gacgaagaaa
    15421 atgttaagaa gtaaaaaaat aaaagagata ttgggattaa acagacgaaa tcaagaatat
    15481 attcgtccat taaactcccc atcctctaaa aaaattgccg ataacaaaat ccttactaag
    15541 aaagtactct caaaggaagg aataaacaca cctgaggtat ataaactaat tagaactaaa
    15601 aaacagctcc aattcttaga ctttgaatct ttaccaaaca gtttcgttat aaaaccaaac
    15661 caaggaactg gtggtaatgg tatagttgtg ctctatggta aaaagaaagg tcagacagct
    15721 tggataagac caagtgggga gataatgagc acaaaggaca ttacaatgca catagagaat
    15781 atccttgaag gaagatactc catgggttca agaaaagata tcgcgattat agaggagaga
    15841 gttaaaacag atccatttct taagcagtac tcttacaaag gtgttccaga tattagaata
    15901 attggctaca accaagtccc cataatggcg atgatgagac ttccaacgaa aaaatctaac
    15961 ggtacagcaa atttacactc cggagcaata tgtacaggta tagatattag ctctggcata
    16021 accacctact ctatgcatat gcaggataaa agtttgataa gcgactcata cgaaatgata
    16081 gattcaacat tagatcttaa agaaaacaaa aaacttgcag gtatccaaat cccttattgg
    16141 gagcaaatac ttgagatagc tgttaagtgt caaaaagcta gcaaattagg atatatcgga
    16201 gtagacatag ccctagatgc agaaaaagga ccaattgttt ttgaattaaa tgctagacca
    16261 ggtcttggta tacaagtagc aaaccaagca ggactaagat ggaggcttga gaaagtaaaa
    16321 gatattgaaa ttaaaagtat gaagcacggt ataagagtag ctaaaaatct cttcggtgga
    16381 gaggttgagg agagtataga ggctattagt ggaaggaagg tagtaaatat caacgaaaaa
    16441 atctttctct actataagac acctgtatct aagaagaaaa atgatcctca gaatcgtgaa
    16501 gccataagag tattcgtaga tacaggctta ataacctccc gtataaacaa aaccctcgct
    16561 tctaggatag gcttttttga agcaattaag tacttctact ctcttcccat tccagacaaa
    16621 tttgaaagct tcaaagatgc tcaagagtac atagattcta accaaacaac cattataggt
    16681 cacccggaca ttcttagagt cgctaaaatt gtagaagagg gtaccataaa gataagacca
    16741 gtaatatgtg tgcacaccaa aatagctagc gaagagaaag aaatagagat ggtagtaaca
    16801 gaagatacca catataatgt agtaatagga agaaacgaac ttaaaggata cttaatagat
    16861 acatctaaga catttacata gtatgaaaaa atacgaagag tatttaaaag aagcaggaaa
    16921 tgtacatttg gcttactatc tagaagctgc agatgagctc ggtatagaat atgagattgt
    16981 tgtgcgtagc ctacttgcta gatttacata ccagggaaaa cattggttca taatcaacac
    17041 cgttactcca ctaactccct ccccaggaac caccatagca aaaagaaaat acctgacaaa
    17101 ccttgtactt agtagagcag atatagacgt acctagacaa caagctcttg gcagtgctat
    17161 agacgccatc aaattcttta ataaatacaa aaatatcgtc attaaaccat cacaacagct
    17221 aggaggcatt ggtatcacca tgcttccaga aacagaagag caggtaattg aagcatatga
    17281 gagtgcattc caaaaaagca atgccaaagg agcagtaaaa gtgctaggcg aagaattcat
    17341 aaaaggagaa aattacagat tcctagtagt tggtgatcaa gtcgtaggaa tagtatggag
    17401 aaaagcccct acagttattg gaaatagcaa gaatactata agggaactaa ttgatattaa
    17461 aaacaaagaa cgccgaatgc ttgttcttaa acctattcct atagacacag aggtagaaat
    17521 aagactacga aaccaagaac ttacccttga ttccatccca gaggaaggaa gagaggttgt
    17581 tcttagatat aactgcaacc taaccactgg tggaactaca gaagaatgtt cttcaattgt
    17641 cgacccatac tacaaacaac ttgcaatctc agcagtgaaa gaacttggtc ttgaatttgg
    17701 tggcgtagat atcattgcac aggatattac aaaacaagat aagtgtggca ttaacgaaat
    17761 aaactacaac ccaggtctta gattacatta caaggtaaat aaaggccaaa ttgtgagagt
    17821 agcaatcccc ataatggaat acattagaga taaatatata aattcagtca aataaatatg
    17881 gtaaaagtat tacaaggatt aaatctagaa aacaaaacct ctgtaataga gattaatgca
    17941 attaaaacag acaaccttga gaaacttctc caattaatac actctttcca ccccatattt
    18001 ctggaagaat ataaatacga agaaaataca cttaagatct actctaactt accatttctc
    18061 tgggaagaat taggcaaagt actagaacag gatttaccat acaacaaaac attagaacaa
    18121 tgcctagaaa ttattaaact ccgagtctcc tctatgtcca ccatacccct actctttgca
    18181 gcacacaagc ttggctacga aacaactcca acgatgacaa agaatgaatc attaacatac
    18241 acaccctctt ttaaccgaca ctacacagta ggatgtggaa aaggttctga gattatctac
    18301 tccatatcct cttcaaaaga ttctaagatt gcaaaagaaa ttcaaagaga taaatggtca
    18361 agtaatcttc tcattgaaaa attaggacta ccccttccag cttgggaaat catagattca
    18421 tttaccgaga tagaagaaat ctggaaacgc tttgacaaac cagtagttat taaaccaact
    18481 ggtctaactg gaggaagtgg tgtttgcata ggaattaaaa cgattgaaga agcaaaagaa
    18541 gcatataaat atgccaaaca agtgatagat accaaaatca gaaaagattg gcaaaccaag
    18601 gtaatgatac aagaacaggt aagcggggaa gattacaggc ttttagttat agatggccat
    18661 ttagaagtag tcacaaagag aatccctgca tttgtaattg gaaatggtaa agacacaata
    18721 acaaagctca tagaagatac taacaaagat ccaagaagag atacaacaag tccagcacac
    18781 acattaaaac caattgaaat tgaccaacca ctaagagcat atcttaaaga acaaaatctt
    18841 gaactagatt ctatccctga caaagatcaa aaaatccctg ttagaaaagt tgcaagcatg
    18901 agtcaaggag gtataaccga agatttcaca gacaaagttg gtccagagat taaaagcatt
    18961 gtagaaagca tcgcctcttc tatccatgcc tttgcattag gtgtagatgt aatgtgcaaa
    19021 gatatttcaa aacctcttac taaagataat ggggctatct tggaaattaa cactatgccg
    19081 gaagcatatc ttaatctctt cccagtaatt ggcccagatc gaggatatgt tgcagataca
    19141 tatattaaaa agctcctcac aaacaacaaa acaaagaaaa ttgtagtcat aggtcattct
    19201 tcctacgata tccccactac attaaaacag aaaacacttc tttcttccta ccttcgagac
    19261 gaagatacag taggagaata caaagatggt gaaataagaa taaactcctt agtattaaac
    19321 aaaggtttaa caaaaagaca gggtatagaa gcacttaaac tcaacgcatc tttagatgca
    19381 atcataattc accacagaaa ttgggaagaa gtgataaaag atggtctggg tttaaataaa
    19441 atagacctgc taatggtaga atcttctcta aaggagaatt cggaatatat gaagataata
    19501 aaaaaataca aacgaaaagg attaatatct aaaataaaaa tattccaata atgaaatatc
    19561 tagtcataga taaaagacca aggattaaaa agatggaaat accttcagct tcttccagaa
    19621 tattggagga gttaaaaaag aaagatatac cagcagattt tgtttacaat gatgaaatag
    19681 agatcctttt catggatggt tccacacaaa tcaaggctaa gggaatagat gtaaaggagt
    19741 acacacacat tatttttaga ggccatgcac ttcataacga taagcagtat gcactcaaaa
    19801 gatacatcat agactacatt gagcaacata acagtgacaa cccagaaaaa aaagtgctca
    19861 tacaaaattc caaagcaatt aagaagttgc catattacaa taagatagcc atttctttgc
    19921 tatgttcaca atataacctc ccctattttc agacatatta cagaacagat ggtaaatatc
    19981 tagaaccaag agatatgtta aaagagtatc ctctaatcat caaggagtat gctggtatta
    20041 atagaattga gataatagac ggggaagaga aaattaaaaa gaatgttttc aaattagaaa
    20101 aagatgaaga ttttgaacag gatggattga aagatcagga tttaagtaat ttctttttac
    20161 aggaattctc taatactgca gaggattaca gaatatttgt taaattagga aaggttattg
    20221 gtggatggaa aagaacagca tcaaaaggat ttatgacagt aaataaagga atatacgagg
    20281 catataatca accaaatgaa cagatgagag agatagcaga gaagatggca aatatactag
    20341 aggctgattt tatggctgta gattttatgt atatgaatgg taagccttgt atacaggaaa
    20401 taagcttaca tccagggttt aaagcctacg agacaaagat aaagggagag catattaata
    20461 tagctgaagc tataattgat gcattctcag attaaattcc agcctttcta aatatatcta
    20521 tccagtcatt cttacttgga atcttacttg ctttaggtat taacccatcc tctacccatt
    20581 cacattgttt aaaactaatt tttccagaat ataatttctg atacaaagtt ggatcctctt
    20641 ctaatttctt cataactttt ctaaatcctc tgaaataaac aacatccttt gcatagatac
    20701 caggatatga agtatctgac aaacccctct tcaccctata tgcgatatca aaagccccta
    20761 gtttagagaa attacctaaa agaacattgt aaagctgcct aaatgacaac tcctctccta
    20821 catatatagc ccatgttaga ccagctttat tccttagtga tttctctggt aaaagtccca
    20881 tattattctc attccatgaa gccaaaccct cctctacatc caaataacta gcaagatttg
    20941 gtttacttat agcctcaaac ccactcttaa gaccgtaata cgaacgaaga gcatgtgtac
    21001 ctacctcatg tattaaagtt ttcctaagtt ggaacttact cttactaata ttcccatcta
    21061 caagcacttc ctttctcttg acccctacct tcactccatt tttggcaata ttaagagact
    21121 tgtttacaga ccaatcctcc aaacccaatt ctctaaatac aacatcaaag acagaaacta
    21181 tactgtcata ccctaacatt tctggatctc tctctttatt ctcttttgcc aaattatatc
    21241 ttccatacct acctcgcaat accctcgctg aatttctaaa aagtttggca gaaggtttac
    21301 catatctagc aatagagata tctgttactg cctcattatt tcctactgca tgcataaggt
    21361 cacttgcttc tctcttggaa tctatgaggt ctatgtaaaa attggaaatc ctaggatcta
    21421 catccgaaat ctcctttact gatgcaagat ctttgaaaat ctcctcgttt ttatttttga
    21481 ctactctgta ttgaaattgc ggttcatatg tatccgaatc gaagaacttt ttcttctctg
    21541 aattcaagtt ggtaggagta aatactaaag caatgggaat ttttaactct cccaaaagcc
    21601 ttgtaacttc ttctaccttc atagttttct tggcttttct aaaaaacatg ttcatagtta
    21661 atatttacat aaatggaaat aaaaaggaac tatcttctaa actctcggtt aaagctgaat
    21721 tttctgtcac ctgctgcaag aagttcgagt gctataccag tacctttggc tacacaaaaa
    21781 gctggatcat ctgctacatg agatggaaca cctgtagctt tagtgaaaag tctatctaaa
    21841 tttcgcaaca ttgctgtacc accactcatt accatacctc tatctatgat gtctgcagaa
    21901 agctctggag gagttttctc tagaacagat tgaatactct ttatgatgct tcgcagaggt
    21961 ctctctaccg ctttggctat atcatttgag tctatgacta tggattttgg catacctgca
    22021 cctacatctc ttcctcttac ttccatctcc tttggatctt ttacttcaag agcggaacca
    22081 atcttaattt ttactctttc agctgtctgt tctccaatta acaaaccttt tttctttctc
    22141 atatacatta tgattgcctc atttatggca tctcctgcta ctctcagaga ttcatactct
    22201 acaattccat ctaaagacat tactgctact tctgttgttc caccccccat atttatgatc
    22261 atgttgccag aagatgtatg gattggaagc tctgctccaa gtgcagcaag tagtggctct
    22321 ggcataaggg ttatttcttt agctccgaca gcatttgcag ccttaagtac tgctctttcc
    22381 tccacagagg taattccagc tggtacactt attactacat ctggtttaaa gaatctagat
    22441 ttaccgagtg ctttgtcaaa gaaaaatcta agtaatgctt cagttactct ggaatctgct
    22501 ataactccat ttcgaagtgg tctttttgca actatattct caggagtttt tccaatcatt
    22561 tcttttgctt cgttaccaac agctaccact gtaaatttat ttacatctat ggctacaaca
    22621 gttggttcaa caaaaactat tccctctcct tggacaaaaa ctactgaatt tgctgtacct
    22681 aaatctatac ctaatttctt tccaaacata acaaaatttc actataagtt atctattatc
    22741 aaatcccaat tccattgcct ctttcatttg ctcaacaata tcttttgcta ttacctgaac
    22801 ttttttacta ccatccaaag ctttagccaa aagctcatcc ttcattccct cagcttcaac
    22861 ccttctctct ctaattggtt tgagatattc attcataact tcaaacaatc tctccttaac
    22921 ctctacatcc tttatttctc cctttctgta caaatccttc atttcttcta cctcttcttt
    22981 gttgctgtta aaaagatcat ggtacatgaa tgcaacattc ccctctattt tacccggatc
    23041 tgttgcatga acccttgcag gatctgtata tacctgaaaa accttctttc gtaattcctc
    23101 ttcactatca cttagaaata tacaattatt taaacttttt cccatttttg aattgccgtc
    23161 tattcccggg acatttacct ctgttccaaa caatgcctct ggctcatgaa gaagtttaac
    23221 gccatatgtg ttattaaatt ttctagccaa aaccctacta tcctctatta ttggggcctg
    23281 gtccttacca actggaacaa gatctgcatt aacacagaag atatctgctg cttggtgaac
    23341 tggatatata aaaaaaccca aaggagtaga attctctaaa cccttctggg caatctcagt
    23401 ttttattgtt ggattatgtt gtacctgttg aacagtagca aaattagata tgtacataaa
    23461 tatctcatgg atagctggta cttcactttg tataaatact gtagccttat taaaatctat
    23521 gcccgcagca taataatcac aaagtaattc ctgaatattc tctttgacct tctttggatt
    23581 gtcaaaatta tctgtaagag cctgcacatt tgcaacaatt acatactcgt catattcatc
    23641 ctgtaatctc accctgtttc gtaaagaccc cattaaatga ccaatgtgta atttacctgt
    23701 tggtctatcc cctgttaaaa ttcttttctt gtcattcata atcttaattc taacaaaatt
    23761 atcttgtatt atgctatact tttcgaaatg tcaaaagaac atataatatt aaaaaataaa
    23821 atactccctg ctctgcttcc cattcttacc tccatcatta tattactagg agctacaata
    23881 atctatcttt attcaagcgg atatagaata gatattctca aaaaagaaat taacataacg
    23941 ggagtcataa ctgtacaaag cgatccctct gccacggaca tgtttgttaa tggtgagaag
    24001 atcggtagag caccaagaag tcatgtacta aaggttggag aatatgatat ttcactcaag
    24061 aaagaaggct atcaagattg gaataagaaa gtaaaagtgc tggaaggaaa atctacaccg
    24121 atattcccat atttggttta tgacaaaata ataagcaaag agaaatggaa atctgaagga
    24181 ttgattgaaa agctatggac aagtgacact agaaatgacc tactattttt gcagaaggat
    24241 tctaacacct ctttctccct atggaagtat gctattaatc ctctactttg ggatttttct
    24301 acgaatccat ctaagatatt aactctagat actaacaaaa tctccctctc tatctctcca
    24361 gatggacaac aagcactctt ggtaatgaca gatccaaagt ctcctcttta ctatttactt
    24421 aatacacaac aaccaaacac tctacaaggc tcaaaccaac ttccctttgg taactactcc
    24481 aaatatacaa tgtcctggag taaagatggc aagtatatac ttctggactc ttccaaagaa
    24541 ctcctgtcat acaatctttc taaccagaca tttacaacaa tcctaaagaa agatgctgaa
    24601 acaaaatatg tttgggatac ggatacacaa ggtttcttct acttaataat agaaattcaa
    24661 aatgatgaat tagaagatat ctacacctac actcttacac agaagtccct cgcaggaaac
    24721 aacaacaaga caataattga cagaatatac ttccagcaaa cagatgaata tatcaaagaa
    24781 tacagaaagt ctgcaaccac actacaggag ttctccaatt ctcccgaaag catacaaaca
    24841 gctggagaag tgatagattt agatgtaaat gtaaacggaa aaggcatgta tatatcaacc
    24901 tcactagcat cttactggta cttcatggat acatcaaaat ttatgactat ttcaccatat
    24961 ccaacagaga taatctccta ctctccagat atttacaaac taggagttaa agacactacg
    25021 ggatacaaga tatttacatt ctataaagag gaaggagacc atacaataga tcttggtgct
    25081 aaatcattaa ataacattaa tacaaacttc ttctggctat ctaactcctc ttacatttat
    25141 cttaaagaaa acaattctat atacatcgca gatatagacg gtgagaataa atatcaactt
    25201 ctagaatctg agaatgtact cgaatacatc gttaataact caagagaaat acttgttaca
    25261 tttgagaaag atagtacagg aaaattagta atcattgaaa acaaattcca ctaaagtacg
    25321 catccggccg gaatcgaacc gacaacctac gcgttaggag tgcgttgctc tgtccttttg
    25381 agctacggat gcatgtataa atataccaca tttacaaaag taatttaatc agctatagga
    25441 taatagatat tatcatttaa tgatataata aaggttgcga tgaaacagga atatataaaa
    25501 ataaaaggag caagagagaa caaccttaag aatatatcag tagatatacc taaaaacaag
    25561 ctagtcgtat ttaccggtgt atctggaagt ggtaagtcta ctcttgcatt cgacaccctc
    25621 tacgcagagg gccaaagaag atatgtcgaa tctctctctt cctatgctag gcagtttcta
    25681 ggagtaatga agaagccaga tgtagattca atcacaggtc tttctccagc aatatccata
    25741 gaccaaaaaa caacaggtac aaaccctcgc tctacagtcg gaaccatcac agagatatat
    25801 aatcacctcc gattactcta tggacatata ggacaacccc actgtccaaa gtgtggttct
    25861 ccaattcaat cacaaaccat tcaacaaata acaaataaag tgatcaagca gatttccaac
    25921 acctcagagg ttacacagat tgtttcaccc atcattaaaa acagaaaagg tgaatttact
    25981 gctcttttga acaaactcct ctcaaaagga ttcttaagag tcattatcga tggaaataca
    26041 tatcttcttg acgacataga agacataaaa ctagaagaga acaataaaca cacaatagat
    26101 ttggtaatag ataggataga ttcaacaaag ttagataaga aaagactcac agatgccata
    26161 gagttagcaa ttacacagtc taatggtgaa gtaaaggtat tattaaaaga caaagaactc
    26221 tttttctcag agaacaatac atgtccgaaa tgcgaaatat cctacccaaa gattacccca
    26281 gcatccttct cattcaatgc accagagggt gcatgcccaa agtgtgcagg attgggtgtt
    26341 ttgaaagaaa tagatatcaa tttgatatac aatcctatct tgacaatact agagggtggc
    26401 atttttccat ggagtaacag aactagcaaa gactcatgga cattgcgaat attaagggaa
    26461 atcgccaaag aacacggctt taacctcact acaccaattg gtagataccc aaaagagatt
    26521 tttgacttaa ttttctatgg gaatggaaca aaagatgcat acacaatcga atacacaaat
    26581 agattcggta gaactagact ctatgaagct aaatacgagg gtgtcatccc agaattagag
    26641 aggagataca gagaaacatc ctcagattat gctcgttttg aaacagagaa gtacatgaga
    26701 gagaatatat gcccagtatg cgagggaaaa cgacttaaac cctactcctt ggcagtaact
    26761 atcgggggga aaaatataga tgagattact aatatgccta caaaagatgt cctaacattc
    26821 tttaagaccc tagaactaaa aggaagcaag aaagatattg cagaaccaat aatcaaagag
    26881 ataaatcaaa gattaaattt tctagaaaat gtaggcttaa actacctttc attatctaga
    26941 aaagctaata cactctcagg aggagaatct caaagaatta ggttagcttc tcaaatagga
    27001 actggattat caggagtcct ctatgtccta gatgagcctt caattggtct gcatcaaaga
    27061 gatatttcaa aactcatcac ctctcttaaa gatctgagag atggaggcaa tagcataatt
    27121 gtagttgaac atgacgaaga gatgacaaaa agtgctgact ggattctaga tatcggtcca
    27181 tttgcaggca agcacggtgg ggatatcgtt gccaaaggca ctttagacga tattaagcac
    27241 agtgactcgt cgactgcgaa gtatctcaac aaaaccctct ctgtcgggca agacgctcct
    27301 cttcttcaag ttggtgcccg tggaaacgag atttctttaa gaggtataaa aactcacaac
    27361 ttaaaggacc tcgatctaac tatcccctta aaccaactaa tctgtataac aggcgtttct
    27421 ggcagtggta agtcctcatt gatagcagac accctttatc cagtattagc aaacagaata
    27481 tcacactcta agcttcaaga gggaaaatat gactcaattg agggtttgga acatataaat
    27541 cagataataa acatagatca atcacccatt ggaagaactc ctcgctcaaa tccggctaca
    27601 tacacaggcg tatttacaaa aatcagagaa atattcgctc aaactaacga agcacaggcc
    27661 cgagggtaca aagctgggag atttagcttt aacgccaaag gtggaagatg cgaaaaatgt
    27721 cgtggtgacg gcgttttgaa gatagagatg caattcttac cagacatgta tattacctgc
    27781 gatgagtgta atggtagtag atacaatgat gaagcacttc aaatagattt taaaggcaag
    27841 aatatatccg atattttaaa gatgaccgtt gaagaagggt tagaattttt caaaaacatt
    27901 ccatctatac atcggaagct ttctgttctc aatgaggtcg gcctcggata catagaactc
    27961 ggacaatccg cccttaccct ttccggcgga gaatcacagc gagttaagct ttccaaagaa
    28021 ctttctaaga tgtctcgagg gcatacttta tacatccttg acgaacccac tacaggatta
    28081 cactaccatg atgtagacaa acttcttgta attttacgaa atcttgttaa aaagggtcat
    28141 acagtaatca tcgttgagca taatatggat attgtccgac attcagattg gataattgat
    28201 ctcggaccag agggtggaga ccaaggaggc tacattatag cccaaggtcc agtctcggac
    28261 atcataaaaa gcaagacaag ttggacagga aaatatctta aaaaatacat tagtgaaact
    28321 tcaggatagt aaactagata gccatacttc ctgtacatca aaatagccag gtataaaaaa
    28381 taaccccatt ttgtattttc tttttactaa aaacatttcc caaagataag acgatacaaa
    28441 tacaaaactt ctaaatctgg gaaagttcag atagttttaa caaaatctgc attactcttt
    28501 tttgaaattt cctaatgttc cacttagtac gataccgaaa acacccactc ctccttactt
    28561 tcttttccac agattataca cttaccagag gagcctatac cttccttggt gagacatctt
    28621 gtggtagctt tggtttcact ctttatcttc tcttcacact cgctgttacc acaccaatgt
    28681 gctaatacaa atcccttctc actgctaagt acagccttta gctcttcata tgaccctact
    28741 ctatgggttt ttgatactaa aagctccttt gatttactca acaaagagtc ttggatgctt
    28801 ttgagcattg tctctacagt atcctctatc ttttctatgc caacattctc tttttctcca
    28861 gtatctcttc ttacaagaac cgcttgactg ttttgaatat cttttggtcc tacctctatt
    28921 cgaacaggaa cacctttctt ctcccattcg ttgaatttaa atcctgcaga cacatactcc
    28981 ctgtcatcaa tttcgactcc atctatgtcc tctagactct cttttatatc tttgcagtaa
    29041 cttaatacct tttctttctc atcttcgtta aagataggta taatgaccac ttttacagga
    29101 gatatcgttg gtggtaatac taatccgtta tcgtcgctgt gagtcataat catagctccc
    29161 atcattcttg tacttactcc ccagctattc tgatacggat atttcttctc accatccttg
    29221 tcagtaaaca taattccgaa tgcctttgca aaattctgtc caagatcgtg agaagtgcca
    29281 aattggagcg cttttccgtc ctgcatcatc gcttctatac cgtacgtgtc tacagctcct
    29341 gcaaatttct cagattcaga ttttttgcct gaaatagtct ccattgcaag atattctcta
    29401 gcaaaatcgc tatacatctg aagcatttct gcgacttcct ttttagactc ttcatcagtt
    29461 ggatatactg tatgaccttc ctgccacaaa aattctgctg ttcttagaaa tggtcgagtt
    29521 cttctttccc accttacaat attcgcccat tggttaattt tcaatggtaa atctctccaa
    29581 gaagatatcc atctagagaa agtatcgtac attatagttt ctgatgttgg cctaactatt
    29641 aattcttcct ttaatttaga actttcatct actactacgc cctttccatc ttcagattct
    29701 tttaatctgt aatgggtaac aatggcacac tcttttgcga aaccttttac atggcttgct
    29761 tctttagaaa agaaggattt aggtatgaat attgggaaat atgcattctt gtgaccactc
    29821 tctttgattc ttttgtctaa ctccttctgt attaactccc atatggcata accgtaaggc
    29881 ttaatgacca tacaacctct tacagatgag ttttctgcta gctctgccct ctcgataata
    29941 tctaaatacc attgagaata atcttgctct cttttcgtta tacctttgtt tgacatatgc
    30001 ctattctact atattctttg cacaaattta aactatcttt ccctcaaagc cccaatgctg
    30061 tgcagccttg gcaagtgcag aatctggatt ttgtagtatc cagtcatcta tatttcctct
    30121 gcctaaagca atatctttgg cttggacaac tgcttctaca ccagcctcta taccccatgg
    30181 atggcctagt actccaccac cacactgtat gattatatct tctccaagct gcttaattga
    30241 ctcaccaagg gtccctggat gaagaccgcc agatgctaca tgccatacag gcttcattcc
    30301 gtaccaattt tgtcccaatg tcatcgaagc tgcaggagta acatctaatt gtagtgcatc
    30361 tctgattaac tttggctcac cgtaattttc catcttggtt tttggagcac caccgtgtat
    30421 tgaatctaca cctaaaagac gcattatctt agcaatcgct accatagaca tagaaaaatc
    30481 tgcaatatat cctttgtcgt acactccact tccagagtct ctgtcaaaaa gaccatgcat
    30541 agctctgtgc gcatgtatgg caagtcctgg attttttaat ctcatcgtat gcatagcagc
    30601 gaaaccagta acaacaacat ccaacatcat ccatctccca ccttgtgcct ttatacggtc
    30661 tg
//
LOCUS       DAOQWP010000007        29616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_1843_length_29616_cov_7.752952, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000007 DAOQWP010000000
VERSION     DAOQWP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 29616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29616
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1843_length_29616_cov_7.752952"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..900
                     /locus_tag="PKI16_01495"
     CDS             106..900
                     /locus_tag="PKI16_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53133.1"
                     /translation="MSQGEYEKEISWDNPTYQVIIDYVYPGDKIEYFQEEMENEEYLS
                     QIKELQSVVNALPTSFSDNLGDYLKTTEWYDGIPDKESVAAPLRMLNYTKVEGQPLQC
                     VGFAILLAGLKYEGINFQNVGGAYSFGSGAFPAKTATELIPFELISDEYAEIAATGYG
                     GIALGSKDMSIEDYEAGDFFVRTDIGRLNNGVSPTGGDITFRTGHIGAIIGKKIVDGE
                     VVLLVADANGENDGKIRIFEVTERNFYKIFGNPGTKKFIIRATSSK"
     gene            960..2387
                     /locus_tag="PKI16_01500"
     CDS             960..2387
                     /locus_tag="PKI16_01500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR53134.1"
                     /translation="MKIQFCGAAKTVTGSCYYLDTGDFKFLVDCGAFQGPDQVEKLNY
                     EPFPFDPAEIDAVFLTHAHFDHCGRLPVLVKQGFRGRIISTQPTRDLAKIVLLDAANL
                     QKEEYERNVARVKDGGNVRNPAYDGSQYVEREPLFTEQDVEEVMQLFNVYPYGNSVTI
                     NDKCEFRLRDAGHILGSALLEFWITIETGRVRKIVFSGDLGQPGARIVRDPDMVREAD
                     YVVCESTYGNRIHKDRNETVLELLSALQTAQKEGGNVLIPSFALERTQEILYEINLFV
                     ENRLLSGLPVYIDSPMASKATQVFRQYPDFYDEDAKRLMEKGDDPFNFQGLHTVDSTE
                     ESKRLITKRGVVIIAGSGMCTGGRIVHHLKNNIENSKTHIIIVGYQVAGTLGRRIVDG
                     EKRVSIMGQEYENNAKLHTLGGFSAHADERDLRYWLRSFGHTPKTIFLTHGDEDIAME
                     FAKNINEELQIDVSVPNMNEIFELK"
     gene            2387..3337
                     /locus_tag="PKI16_01505"
     CDS             2387..3337
                     /locus_tag="PKI16_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-2B"
                     /protein_id="HNR53135.1"
                     /translation="MMEKFGEIEKIAEDIKGLKVQGATNVCIATFEGMKLYLELSKTQ
                     DLEILKDEFFGVGEQLANARPNEPLARNGVHYVKYFFKQKHSDISQVNSAKEILKELC
                     DEYLGLITAAKKSSIEKCARYLTNYDKILTHCHSSTVVDLIKEISKEDTNFEVVCTET
                     RPLYQGRITAKNLVDAGIKTTLIADSAAESFAVNRGSVPMDVVFLGSDQIMKDGYVIN
                     KIGSWGVAVAAHYGGKPLYIVSNLLKFDKDSLFSDVEIEIREDRELWPEAPRNLDMYN
                     PAFEIVDNALITGFVTELGIIKPNEVEKVIKENYPWLFNE"
     gene            3356..4870
                     /locus_tag="PKI16_01510"
     CDS             3356..4870
                     /locus_tag="PKI16_01510"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF003338.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNR53136.1"
                     /translation="MSYYLKSKSLDLGEDNDFNVVLNSEDAKRMGIREGETLMIGIGE
                     VELYADVFETDTLVKEGEIGLYEEIWENYNVEEGSRIYADIPERSKSLEAISSKLLGH
                     SLSKKDLELIMDDIGSKKLREVEVAFFVSTFFNPGFNDDEILWMTEGMAESGDKLSFK
                     DLVGKDGLVVDKHSIGGVAGKGITPTLVPILVSGGLLVPNTSTRAITSPAGTSDILEV
                     VMPVKLTKEQIMEVVKKTGGCMFWGGSLSISPADDIIINVERSLRIQEFQKVLVSIVA
                     KKIAMGVNHVLIDLPYGKGSKLEGIEEAQMLDREFKKLFLKVGIECETIKRIVKGPDG
                     PAIGPNLEIKECLKVLERADDRSKALEDVVLNMAEKLFESTEKVPRGEGKKYAQQLLE
                     SGKALDKFWEIGFAQGATKKVKSSEISSGKFKFDVLSTTNGVVSMVDNSELVNIARAL
                     GNPKIKEAGIYMYKLNGDKVSKGDVLMTIYATSNGRLENGKKAINLDKLYCISC"
     gene            complement(4850..6715)
                     /locus_tag="PKI16_01515"
     CDS             complement(4850..6715)
                     /locus_tag="PKI16_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNR53137.1"
                     /translation="MKKLTLTILTLLLFLLPISPVLAATYDFVIDTDTQISYTTGDNF
                     VTVKYTYKRTVENSSYYYPASGEKVFHIPDIQNKTDEEINVERKFKKDSIVVKDQYGN
                     KIKYTVKEEANPNGIYISIPNYKTTTKNSPYIITFTYKTHDYVQKVKDFVSIQGPALP
                     KDIVLEQTDKSSGTKTKYNYNLSIVTDKNISPLAKIYPSDFTKKEDSKTITYSFNQLD
                     RIENSPYLEFGTEAIYKFELLFKTPKTDTFIPEKYSDIYSQLSTNIYEISLPREYGET
                     NQRVFFSEVIPSPKDIYQDTEGNIIGVFEVPANLDSEISITGYIFVEQDSFETPVDSI
                     DMNLTEYFDIVKKSSFLDKYLLPTKYWQSTDSYIKQEADKLIANQSTILGVIKADYAF
                     VGDKLEYDYNKANSENERIGAKAALTGGDSVCMEYSDSLIALLRAQGIPARAAGGYAN
                     ISEEIVDNPATHQWVQIWIPNYGWLTADPTLESKNMLIGKSIDRILWETFNGDSLSNI
                     RVYSANDITSVSNGGYNIRIYGADDINESELKRYIDLVPDKGYGSTEELPVNSNYSLS
                     HWFNTILKTTVIGKSILIAGPILLFLAVLTGAILGISLGVKAAKKKKQQSTTNTV"
     gene            6764..7858
                     /gene="ychF"
                     /locus_tag="PKI16_01520"
     CDS             6764..7858
                     /gene="ychF"
                     /locus_tag="PKI16_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008286790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNR53138.1"
                     /translation="MSVKRSHSLSLGIVGLPNSGKSTIFNALTKMSVPAENFPFCTID
                     KNIGVVEIPDERLMKMAEHFNAKKIVHSAMTFVDIAGLVKGASKGEGLGNQFLGHIRE
                     VDVIMYVLRAFQSSEIVHVYERVNPVEDFEIVQAELIFKDIEVIEKKIAMVDKLARIG
                     HKDAEEELKILKKIMEELNKGTAVIDMELSDDEKEYIYDLWLLTNKKRMFILNIKEGM
                     EEEQVKEWERQLKEKTGDMVVIADVKLIGQGEEEIAEYEELLGYKPNSVVDIIKKAYE
                     CLNLITFYTGSEKECNAWTIEKGANVKEAAGVIHTDLEDNFITADVVNLEDMLSVGGW
                     NSAKEKGLVKNHGRDYLVNDGDYIIILANK"
     gene            7946..8302
                     /gene="rpsF"
                     /locus_tag="PKI16_01525"
     CDS             7946..8302
                     /gene="rpsF"
                     /locus_tag="PKI16_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNR53139.1"
                     /translation="MVVKETKKLNGYEMAVVLKPLLPDDVRKSIHKEFVDMISKNGGE
                     VLDVDVWGKRYLAYKIKGHNEGYYIIYTFKVSPKEILELKRQMELRQEVLRYMVVTID
                     DVTLAGKRIKKKEIEI"
     gene            8365..8853
                     /locus_tag="PKI16_01530"
     CDS             8365..8853
                     /locus_tag="PKI16_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNR53140.1"
                     /translation="MAARSLNKVILIGNLTRDPEMRYTNSGTPVVTFGMATNKSWKDA
                     GGETKESAEFHNIVAWNKMAEICQQLLAKGMKVYIEGSLNTRSWEGEDGVTKYKTEIR
                     VNDMILLDSKGKQGAGMDSEEGAKEEKPVKEEVEEVVEEVAKEEEAPAEDQDPLEEDL
                     PF"
     gene            8871..9176
                     /gene="rpsR"
                     /locus_tag="PKI16_01535"
     CDS             8871..9176
                     /gene="rpsR"
                     /locus_tag="PKI16_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNR53141.1"
                     /translation="MSEEVITKVEETTTDSSYGGDRKKNNRRRKKPLQNLKCPLCESG
                     VKSVGYKDVYQLKKFTSVRGKIISTEKSGVCHKHQKQLTNSIKRARYLALLPYSSDE"
     gene            9265..10626
                     /locus_tag="PKI16_01540"
     CDS             9265..10626
                     /locus_tag="PKI16_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53142.1"
                     /translation="MSKLPILQKNAYLSLVVKNNSIWAHLAYTDHNAGREYILSDFTD
                     LNPLKQRLDDEFFTRKFWYEYFDNLEKVFNWDIVDRDTYRLQMYKTFKDEGVGLTGIR
                     VLVDENQKYFNTIFSSLREFSKDIAVRIVDDSYIYNLLEGLLGRMEYDDILYVDMDLL
                     DFKIYRGRKIIENKKEKTIITKSKISWGNEYGVIDSVRDSRFKAFLASDFASKDILNY
                     WSNFVLNKIFFTEDPNILDILRSYTTIQNYSLYQDNTDKLSGFGTDSTNSAMIISGHI
                     PTILGKQKTLMTVVDGLEIMGELDCIWDLEEKILSYGKSYIFGTNSTDIILTRKEIFS
                     PSTKVLIPEVKSLKNQNKVIMSGYTESLSMEKNEFYALSPEFTFIQLPKHEDKLVVEM
                     EFKKDIVIPNGVKSKVGFVSIPGKGDYDSILVDGRPRPIVYGPDVYTNRSKLSMWLND
                     FKA"
     gene            10610..11629
                     /locus_tag="PKI16_01545"
     CDS             10610..11629
                     /locus_tag="PKI16_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53143.1"
                     /translation="MILKHKLFEKYDEYIFKIPSGRGDRILVNTGEMLKEGTDILVRR
                     GNNVKNSFFLPSQLGCAKENIGKYLMCMDGEFVEKGDILAEKTSMGGLTIKRLTSPIQ
                     GVVDMSRIGIGYLDLLGEEQENIVKSTFNGVVESINPIDGITIKASAYALDLVAISNV
                     QEDAKYIVGEFVSLGSGNDVILKSVEEDLQDKIVFAGKRLHPDLLRDIFEKGAKFVLS
                     YSMDYQNFRNQGLPVGVIGGFGEIYSPREMIEKITKKDHTFAVVDVNESQIFFLGGNI
                     KDGVADSLFVQSAVGRKVISHALGNYGMLGEVNGVEDDSYLSVNWENGASSIINVGSV
                     EFVSY"
     gene            11646..12854
                     /locus_tag="PKI16_01550"
     CDS             11646..12854
                     /locus_tag="PKI16_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNR53144.1"
                     /translation="MDKNKSLQKTLGLAILLVCIFVLGFVSGREFQGKDILNDNKEKV
                     DTTMYWDVWDILRSNYVDSSEISPQDMMYGSIKGLVNSYNDPATVFLTPEETEEFKAT
                     SEGKYFQGIGAELGYENGNIIVVTPLDGSPAKAAGIRAGDYILSVDDKDITAEDTVYD
                     VVARIRGEEGTKVKLKILHKDSNKPVEVSIERKQITVASMNVEFLENKSIALFRVNRF
                     TEASYSEWVKEWNTKVKEIKDAKVNKVILDLRGNPGGFFDAAIYAADDFLQGGYTISM
                     QQDGKGKIDKYESKDGGNLTEVKLVVLVDGGSASAAEILAGALQQADRATIVGEKTYG
                     KGTAQNVIDLSDGSSLHITTLKWLLPNGNWLNKEEPITPDVIIEFTDEDFIAGKDPQL
                     VEAIKQINKL"
     gene            12866..13996
                     /locus_tag="PKI16_01555"
     CDS             12866..13996
                     /locus_tag="PKI16_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNR53145.1"
                     /translation="MKKFKEIFSKKVLYIIGFVLAIVMILLIGGYLLLRNAESIKNSF
                     SFLFNSENTCQESDKLIVSESEANTIKIVNESQASVVSIAVSTLSVSKTEGIVDSSSN
                     IGTGFIIDSSGLILTNQHVVSSDTAVYQVITNDEKKYDIVEIVRDDVNDIALIKIDAK
                     DLTPIKLGNSDVLAVGQKVLAIGTPLGKYAGSATEGIISGLDRTVTASSDWTGFTSKT
                     YEDVIQTDAAVNPGNSGGPLINSQGEVIGINFATTSGADNISFAIPINKVKERVEEYK
                     TYGKFIKPYMGISYEMFYKYDLLYYTNIVPGALVKGLDTTGPAYKAGIRRGDVITKFG
                     GEDVNIALINLIQKHKVGESVKVEVYRNGDTKSFDVVLGEAK"
     gene            complement(13979..14749)
                     /locus_tag="PKI16_01560"
     CDS             complement(13979..14749)
                     /locus_tag="PKI16_01560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNR53146.1"
                     /translation="MNNDLALLYKIQSVLKDSGYHDITRISQEILTYIRSNKIPPEEI
                     FNRLLSNEPWEYISGEAEFRGYKFNVNSNVLIPRVETEQIVDIAKENLKGIKNIVDIG
                     TGSGCIAISLAKELNNNISIYAIDISKNALDVARDNAKLNNVKEKIIFKQNNLLKGME
                     FEPSTLYIANLPYIPTDMYNTLDSSVHDFEPKLALEAGEDGLKYYRTLFVQIPKGNNL
                     LIVEIEPSTIKYITNIKEPIKVIQDFRGLNRFLLFGFS"
     gene            complement(14742..15833)
                     /gene="prfA"
                     /locus_tag="PKI16_01565"
     CDS             complement(14742..15833)
                     /gene="prfA"
                     /locus_tag="PKI16_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNR53147.1"
                     /translation="MDITSLQTKYSTNDAQTKVIEIQNQISSSHNSGTNITQLSNDLS
                     YYSELSSLISKIQRCLKEINDAQELLEDENDPFIKEMATEEIENKEQELEKLELAIQK
                     LKIAREFEDEDDNRSAILEIRAGAGGDEASLFAADIFRMYKNYSINKGWNIQVIDSNI
                     TEDGGYKEVIVHIEGKNVFKNLKYESGVHRVQRIPITESSGRIHTSTASVAILPEAKD
                     IDIQINPDDIKLEVMRASGAGGQCVNRTDSAVRITHLPTGIVVSCQETKHQAQNKEKA
                     MALLRSRLYDKKKAEEDAKRSNLRSSMIGSSMRAEKIRTYNFPQNRVTDHRIKKSWFN
                     LESILNGDIQTIIDDVREGIQLQALEKSE"
     gene            complement(16000..16206)
                     /gene="rpmE"
                     /locus_tag="PKI16_01570"
                     /pseudo
     CDS             complement(16000..16206)
                     /gene="rpmE"
                     /locus_tag="PKI16_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            16296..16517
                     /locus_tag="PKI16_01575"
     CDS             16296..16517
                     /locus_tag="PKI16_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HNR53148.1"
                     /translation="MKYSRGIENRIKRVEGQVGGVRKMIMAQEGDTKVMTQLQAAISS
                     LESLKTELIKQQMKESMVGDIKKSLGLSD"
     gene            16592..17239
                     /locus_tag="PKI16_01580"
     CDS             16592..17239
                     /locus_tag="PKI16_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR53149.1"
                     /translation="MKIKRVGWNSFLISSSNISLITDPLDLLQSGVTFPKTKADIVLF
                     SNSDENSKDSILKEKKLESKVVPDTRETIMEIFTPGEYEVGGLMIRRSIGENFYMIDE
                     KNIRVLYLGGTDNSFNPDSVKDVGDVDVLILPVGDGVNFMDFEKLEKVISNIDPSIIL
                     PCAYKEEGSKFEGIKSKDEFIKYFGFANVTEDTTLNVAKKKVEEEQQSVEVIFLK"
     gene            17245..18564
                     /gene="dnaB"
                     /locus_tag="PKI16_01585"
     CDS             17245..18564
                     /gene="dnaB"
                     /locus_tag="PKI16_01585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNR53150.1"
                     /translation="MEEKVPPQNTEAEQSLLGCLLLDKDAVIAVSGWLIPEHFYDERN
                     AIVYSNILELFNDAIPVDLVTLSDRLKKKKMLSKIGGRTYLADLSSTVSTSSHAEEYA
                     NIIKESATRRGLISAAKEVTQYAFDETLGMDEVIDRSERQIFDIAQKGTRTNFVHIKD
                     LLKDAYERAERADRDEAYLGISTGFKDLDDLLGGFQKSDLIILAARPSVGKTSLALDM
                     MRHAAMVEKKTVAFFSLEMSQTQIMDRLLGMQSGIPFWEIRTGRLSDKKFMKLADTMG
                     ELADANLYIDDQAGQHINAIRTKARRLVLEKGLDIIFVDYLQLMHGSNQESRTLEVGE
                     ISQGLKNIAKELNVPVVALSQLSRAIEQRQGRRPQLSDLRESGSIEQDADVVIFIDRE
                     ETWNPDTENKGIADLIVAKHRNGPTGLVKLAFINEIASFRNLYKGDK"
     gene            18586..18669
                     /locus_tag="PKI16_01590"
     tRNA            18586..18669
                     /locus_tag="PKI16_01590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18620..18622,aa:Leu,seq:caa)
     gene            18695..19444
                     /locus_tag="PKI16_01595"
     CDS             18695..19444
                     /locus_tag="PKI16_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNR53151.1"
                     /translation="MEDFILGVLEGAGSIDFNIVWKILLFAFVIFWIVVLDWIWADSG
                     ERTSSKRSRILYVILAAVFNVFGWLIYLIIRPSQTIEEIYWADLERRYLKYETSELGD
                     CPKCGTQLYPGYLYCPSCNYKLKRKCPSCGVYVDKKSNFCPFCSAVIKTAVIEKEIVT
                     PSIEEMQKQNLEVREELTEVVETQGTKYSGGRRSVTAKLGGKIVGGYKIIASSIVKVF
                     KKKEAKKEIQVQPKVKRNKKKHSSKKKKQKR"
     gene            19547..20302
                     /locus_tag="PKI16_01600"
     CDS             19547..20302
                     /locus_tag="PKI16_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53152.1"
                     /translation="MGLIIFWRGSVESRKERSSTFDLFFLSGFFSLVSARIAHIVANW
                     EAFSGFIWYWLPYERYGEEIFLFRLLPWRFLNIFDGGINILVMFVSFLLFATIGVVII
                     KKWKWRDMFPVVFFSAESMLAMSFLFSGVIANNMGWIVQGGVLLIPIILILIILSFVT
                     PNTEGKEEKSLYIIVNIFLILLSTGFILYVYLKNDIELIDKISCLIFLIWSLIGSLSF
                     LREERGSRFSIERVSSVRTISSADINQPIRLSK"
     gene            20308..21273
                     /locus_tag="PKI16_01605"
     CDS             20308..21273
                     /locus_tag="PKI16_01605"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNR53153.1"
                     /translation="MIVTIGRDITSGVRVDIFVLEQLKLMGVEVPSRSFLHKMFKEVI
                     KVNGKEVKSSYKLKNGDVVNISEEKIEQELNKQDKNIGITAQEGKLDIIHETEQYLVI
                     NKPKGITVHPGIKNTENTLVNFVRGYLEKKGEFNPRLNRAGLVHRLDKGVSGIMVFAK
                     TVESQEYLQKEFENHTVKKIYLAKTEGSSELTIYNRNIKEELDTLLKRNFEMDSTWTL
                     YEGYIGRDRRNRLRMKFREEKFPNSKKAISFVKRIGENEFLVSIETGRMHQIRATLAY
                     IGIPIQGDTLYRKTKKDGIPEEIELESILISFKDMDGKTVTFRLY"
     gene            21343..22464
                     /locus_tag="PKI16_01610"
     CDS             21343..22464
                     /locus_tag="PKI16_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNR53154.1"
                     /translation="MATLFLGFVIYYIFTVTSIKSIDKTILNGSQRYLLSQSNGESKR
                     TVIILEEGYGNERRISEVYLFLSNQEKGKSLLVYIPSWLYYTGLEENFGSAIPISSFR
                     YAGDTIQEDRGVEYSVWQISQILGITFNNYIYISSEANSTLDSVFGEDVMVKDRYRDM
                     FKVGDKESLSEGFFRLQALSSKISFVKSLFKVNEFRNFSNNIYSNLSFIEGITMLKNV
                     EKTLQATESYAIDLSDAKYSKEELSDSGGIKRYFLSKEFDNSYRDLISLLIDRELEKE
                     RVRVEVYNGSGVAGAAMQFGRKIENSGCDVVRYENAPDIIDKTIMYIPKLDDFKKSFK
                     IVSEIFSDNFELVEGRPDFMTTGDIVIILGEDIKRMYSF"
     gene            22506..25019
                     /locus_tag="PKI16_01615"
     CDS             22506..25019
                     /locus_tag="PKI16_01615"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNR53155.1"
                     /translation="MITALLLLILFSLVFTAFVLIFAKRNREEEIEIYEDIRNPLVMQ
                     ILVPRENDKSALAAEQMFASIHGILGDSKKSLDLISFEIISSGEDGIRFFVVTPGHLA
                     KFIEGQIYAQYPNANITYEKDYSLNNSELGEEVYVTSGEVEMEKDSIFPIKTFRNFEV
                     DPLAAITGAMSEMGNGERAWLQIIVRPVSNYWQKKSKEYISAVREGKDIGGFNIIAKF
                     RSLISTIGKNLTYSDSQAAKKEVVKLEPGQEEELSEIETKMLKLGFEVKIRIVTKATT
                     QERSEQILRDIVASFKQFTTAHLNNFIHSVPVKTGKEIYEEYLRRYMPKEEEDILNIE
                     ELASLYHLPNISVETPNIAWSRSKKLEPPMNLPKANDGDVTIFGETDYRGHKVNFGLK
                     RGDRARHFYLLGKTGAGKSTLFKNMFISDVLAGDGACFIDAHGDTVEELLEYIPPNRI
                     DDVIYFNPTDIENPVGFNLLELKDKSQRDLIADGVVEVFKKQFGDSWGPRLQYILTNT
                     VATALEAQGITLLAVIRMLTDINYRKFILKQVHDPILYKFWVEEFAQMSQNPRLLAEA
                     VSPIQNKVGRFISSAVTRNIVGQVKSTIDLREIMDNRKILLVNLAQGKLGEETASLLG
                     GMIITRLQATAMERVDIPFEERKDFFLYVDEFQNFATDSFAKILSEARKYKLNLIMTN
                     QYIDQLPLTVRQAIFGNVGTLGSFVVSQADASILEKEFAPVVSADDLVSLDAFSMYVK
                     LCIDGMTSTPFSAKSLPLRYEKFHLKDEIVQKSREKYGVPRAEIEEKIDKWSRQTYSE
                     KGNRSINKEKEESDIRPVADRKYEKKENDIEEKVYKVKI"
     gene            25024..25806
                     /locus_tag="PKI16_01620"
     CDS             25024..25806
                     /locus_tag="PKI16_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNR53156.1"
                     /translation="MSGHSKWDTIKHKKAINDAKKGKVFSKLSMMITHASRQGGGDPE
                     MNPNLRLYIDKAKEAGFPADRIEKAIQKGTGEGGDGVVFEENTYEGFGPFGIQMLVDT
                     LSDNKNRTVAEIRQLFDDIGGRMGDTGSVSWNFEAKGYIVIKSGHMIKSPKFGEEDIF
                     EKEESEAVMLSIMDIEGVLDIVEIEVDGEKGFEIYTEFHQFAHVRDAISKLGYVIKEA
                     IIIKEAKIKKELKPEEVAKVEEAIEKLEEYDDVQNVWTDLVE"
     gene            25811..26290
                     /gene="ruvC"
                     /locus_tag="PKI16_01625"
     CDS             25811..26290
                     /gene="ruvC"
                     /locus_tag="PKI16_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNR53157.1"
                     /translation="MRILGIDPGLATTGWAIVDFNDNGDPTPVDYGALITKKGLTVSQ
                     RIGEIYTDLHELIKKYKPEYAGVETLLFCKNVTTAISVGEARGVVLLVLEQNRIPLKE
                     FTPLQVKNSIAGYGKATKRQVQENVKMICKLKEIPRPDDAADAIAIAIATQVLISRI"
     gene            26297..26878
                     /gene="ruvA"
                     /locus_tag="PKI16_01630"
     CDS             26297..26878
                     /gene="ruvA"
                     /locus_tag="PKI16_01630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNR53158.1"
                     /translation="MISYISGVVQKISFGKDSYLDLLTKMDISYRVHIPQNFAPIEKG
                     QQLSLYTHFHVREDIQSLFGFETEEERDFFEELITVSGVGPKIALAVLSTYKKDDLEK
                     IILEGDAKKLSKVSGLGSKGAQKIILELRGKIDFDKDGKVSSAKSRDLKNALKVLGYS
                     GEMLNEKVDLGEKILDKDESIEIEDLIKRVLSA"
     gene            26878..27906
                     /gene="ruvB"
                     /locus_tag="PKI16_01635"
     CDS             26878..27906
                     /gene="ruvB"
                     /locus_tag="PKI16_01635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012859656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNR53159.1"
                     /translation="MIQSSFVSENIEKIEKAPKKKDAQLEQKIRPSRLEEIIGRKQEK
                     RTLLMMIESAKKRKEVIDHILFYGPPGLGKTTFAMAIANEIGCNIKITSGPAVERQGD
                     LAAILTGLKANDVLFIDEIHRLSRVVEEILYPAMEDGVLDIVMGKGPSAKTIRLNLEP
                     FTLIGATTQIGKISSPMRERFGLVQRLDFFSDEDMVSILRRAAKLWNIKIEEGALEKL
                     ARSSRGTARIGLRLLKRTRDYAESENVKTITEKIVGDTQKLLGIDERGLEEIDREILK
                     CIMLSFNGGPVGLSTLAASISEDVTTLSEVYEPYLMKCGLIKRTSRGRVLTEKGLKYI
                     EGIIYGKS"
     gene            27893..28711
                     /locus_tag="PKI16_01640"
     CDS             27893..28711
                     /locus_tag="PKI16_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53160.1"
                     /translation="MERANVGSDVEKRGKLLLLAGLIAVAMFLNKILQKEASVEIPNI
                     YLNGALYFAISFVGLLWSFNFQIKSKSILYIIQAALFVFSEAIFIEFFFFQKFNRLYE
                     ALILLILLTLVFIGNYVAFLMANVFNVGLFKNIPLVHVGRTSSYLLSLLTIYFVTFSF
                     LSSNLPIYILLPLIVIAYAIVVYIHYLNIGIGGGNLIWKTVLTGLVVLVLFLGVFLTG
                     NSHEIISFVPVVGYFLCVSVVSQSDIEYKERNVFFYLISLAIFFFIAVFINLLS"
     gene            29169..>29616
                     /locus_tag="PKI16_01645"
     CDS             29169..>29616
                     /locus_tag="PKI16_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53161.1"
                     /translation="MKDTFPKLPDQDDFLLNLQNSNYSMQTIYNYARDLCIFAVYLHF
                     INVDFKNITKRDISNYKGYLRAGEHLKDLDKIRSKVLNNVDIPTESKGEAPRDDLRVG
                     NDLGGVNTPLSGSEKEKKDTGVFETDFLGEVYSKVYGGLGIYGKPMN"
BASE COUNT        10255 a         4123 c         6262 g         8976 t
ORIGIN      
        1 ttttactgag atagatccag gaaatcaatc acaaaaaatt gaagacttca tatctctgca
       61 tatcgtgaat atcgaagcag tggtcatacg aaaggttctg gatttttgag ccaaggggag
      121 tatgaaaaag aaataagctg ggataatcct acatatcagg taataataga ttatgtttat
      181 ccaggagaca aaattgaata ctttcaagag gagatggaaa atgaagaata tctatctcaa
      241 ataaaagagc tccaaagtgt tgtaaatgca ttaccaacct cttttagtga caatcttggt
      301 gattatttaa aaacgacaga gtggtatgat ggaattcctg acaaagaaag tgttgcagcg
      361 cctctgcgaa tgctgaatta taccaaagtg gaaggacagc cgttacaatg tgttgggttt
      421 gcaattttac ttgcaggact taaatatgaa ggaattaact ttcagaatgt aggaggagca
      481 tattcatttg gtagtggagc gtttcctgct aaaacagcaa cagagctaat tccgtttgag
      541 ctaattagtg atgagtatgc tgaaattgct gccacaggat atggtggtat agctctaggc
      601 tctaaagaca tgagtataga agattatgag gctggtgatt tttttgtaag aacagatata
      661 ggacgtctaa ataatggtgt ttcgccgact ggaggagata taacatttag gactggacat
      721 ataggtgcaa taataggaaa gaaaattgtc gacggagaag tggttttgtt ggtagcagat
      781 gctaacgggg aaaatgatgg gaagataaga atatttgaag taacagaacg taatttctat
      841 aaaatatttg gaaatccagg aactaaaaag tttataataa gggcaacttc tagtaaatag
      901 ttgtaaactg acctcctttt cctctatcat atacaaatct ttaattaaac tgctcaatca
      961 tgaaaataca gttttgtggt gctgccaaaa cagtcacagg ttcttgctac tacttagata
     1021 caggagattt caaatttctc gttgactgtg gtgcttttca gggcccagat caagttgaga
     1081 aactcaacta cgaaccgttt ccatttgacc ctgctgaaat agatgcagta tttcttaccc
     1141 atgcccattt tgaccattgt ggaagactac ctgttcttgt taaacaagga tttagaggta
     1201 ggataatatc tacacaacca accagagatc ttgcaaaaat agttcttttg gatgctgcaa
     1261 atttgcagaa agaagagtat gaaagaaatg tagctagggt aaaagatgga ggaaatgttc
     1321 gtaatccagc atatgacgga agtcaatatg tagaaagaga acctctgttt acagaacaag
     1381 atgtggaaga agtaatgcag cttttcaatg tgtatccgta tggaaactct gtaaccatca
     1441 atgacaaatg cgaatttaga ctaagggatg ctggacatat cttaggctcc gcgttattag
     1501 aattctggat aaccatagaa actgggagag ttcgtaaaat agttttctct ggagatcttg
     1561 gacaaccagg tgcccgtata gttagagatc cagatatggt gcgagaagca gactatgtag
     1621 tgtgtgaaag tacatacgga aatagaatac ataaagacag aaatgaaaca gttctagaat
     1681 tactttcagc acttcaaaca gcacagaaag agggtggcaa tgtcctaata ccttcctttg
     1741 ctctagagcg aacacaagag attctgtatg agattaatct atttgtagaa aacagattac
     1801 tttcaggact tccagtatat atagatagcc ctatggcatc caaagcaaca caagtgttta
     1861 ggcaataccc agatttctac gatgaggatg caaaaagact aatggaaaaa ggagatgacc
     1921 ctttcaactt ccaaggtctt catacggtag attcaacaga ggaatccaaa agactaataa
     1981 ctaaaagagg tgttgtaata attgcaggaa gtggaatgtg tacaggagga agaatagtac
     2041 atcatttaaa aaataatatt gaaaacagta aaacacacat aattatagta ggataccaag
     2101 tagctgggac ccttggaaga aggattgtag atggggaaaa gcgagtctcc ataatggggc
     2161 aggagtatga aaacaatgct aaactacata ccttaggggg attctctgca catgcagatg
     2221 aaagagattt gagatattgg ttaaggagtt ttggacatac accaaaaaca atatttttga
     2281 ctcatggaga tgaagatata gctatggaat ttgcaaagaa tataaatgaa gaacttcaga
     2341 tagatgtctc agttcctaat atgaatgaaa tatttgaact taaataatga tggaaaaatt
     2401 tggagaaata gaaaaaattg cagaagatat taaagggctt aaagtccaag gtgctacaaa
     2461 tgtctgtata gcgacttttg agggtatgaa gctgtatctg gagttaagca agacgcaaga
     2521 tttagagatt cttaaagatg aattctttgg agtaggtgag cagttggcaa acgctagacc
     2581 gaacgaacct ttggcacgaa atggtgttca ctatgttaaa tactttttta agcagaaaca
     2641 ttcagatata tctcaggtaa attccgcgaa agaaatacta aaggaacttt gtgatgaata
     2701 tcttggatta attactgctg caaaaaagag ttccattgag aaatgtgcga gatatcttac
     2761 aaactatgac aagatactta cacattgtca ttcttctaca gttgtagatt taattaaaga
     2821 aatctcaaaa gaagatacaa actttgaggt ggtgtgtaca gaaaccagac cattatatca
     2881 aggaagaatt actgcaaaga atctagtaga tgcaggtata aagactacac taattgcaga
     2941 tagtgctgca gaatcatttg cagtaaatag gggaagtgtc cccatggatg tagtatttct
     3001 tggaagtgac caaattatga aagacggata tgtaattaac aagataggga gttggggagt
     3061 agctgttgca gctcactatg gaggtaaacc actgtacatt gttagcaatc tgctgaaatt
     3121 cgacaaagat agtcttttct cagatgttga gatagagata agagaggata gagaactttg
     3181 gccagaagca cctagaaatt tagatatgta taatccagca tttgagattg tagataatgc
     3241 attaataact gggtttgtta cagaattagg aataataaag ccaaacgagg tagagaaagt
     3301 tattaaggag aactacccat ggctttttaa tgaataaatt aattactaca taaatatgtc
     3361 ttactattta aagagtaaaa gtcttgatct aggagaagat aatgatttca atgtcgttct
     3421 aaacagtgaa gatgcaaaaa ggatgggaat tcgagagggg gaaaccttaa tgattggcat
     3481 cggagaagtg gaactttatg ctgatgtatt tgaaacagat acattggtaa aggagggaga
     3541 gatagggttg tacgaggaga tatgggagaa ctacaatgta gaagagggta gtcgaatata
     3601 tgcagatata ccagagcgaa gtaaatcatt agaagcaata agttctaaat tacttgggca
     3661 tagcctttcg aaaaaagatc ttgagttaat aatggatgat ataggttcta agaaacttcg
     3721 agaggttgag gtagctttct ttgtatccac attttttaat ccgggtttta atgatgatga
     3781 gatattgtgg atgactgagg gtatggcaga gtctggagac aaactttctt ttaaagattt
     3841 ggtaggcaaa gatggattag tagttgacaa acactccatc ggtggagttg caggtaaggg
     3901 tataactcca acacttgtcc caattttggt atccggagga ttacttgttc ctaacacatc
     3961 tacaagagcc attacttctc cagcaggtac ctcagatatc ctggaagttg taatgcctgt
     4021 aaagctaaca aaagagcaaa ttatggaggt agttaaaaag acaggaggtt gtatgttctg
     4081 gggtggttct ctttcaattt ctcctgcaga tgacatcatc ataaatgtcg agaggtctct
     4141 tcgaattcaa gaattccaaa aggtactagt aagtatcgtg gctaagaaaa tagcaatggg
     4201 agttaaccat gtcctaatag atctcccata tggtaaggga agtaagctag aaggtataga
     4261 agaggctcaa atgttagata gagagttcaa gaaacttttt ttgaaggtag gaattgagtg
     4321 tgaaacgata aaaaggatag taaaagggcc ggatggtcca gcaattggtc cgaatcttga
     4381 gataaaggag tgtcttaaag ttcttgagag ggcagacgat agatctaaag cactagagga
     4441 tgttgtctta aatatggccg aaaaactttt tgaaagcaca gaaaaagtgc caagaggaga
     4501 gggtaaaaaa tatgcgcaac aacttttgga gagtggtaaa gcattggaca aattttggga
     4561 gataggtttt gctcagggtg ctactaaaaa agttaaatct agtgaaattt ctagtggtaa
     4621 gtttaaattt gatgttctaa gtacaacgaa tggtgtagtt agcatggtag acaattccga
     4681 gttagtaaat atcgcaagag cactaggaaa tcccaaaatc aaagaggcag gtatatatat
     4741 gtataaatta aatggagaca aagtatctaa aggagatgtt cttatgacta tatatgcaac
     4801 ctcaaatggt aggctagaaa atggtaagaa agcaattaat ttggataagt tatactgtat
     4861 tagttgttga ttgttgtttt ttcttttttg ctgcttttac acccaaggat atccctagaa
     4921 tagctcctgt gagaacagcc agaaagagta gaataggacc tgctattaga atagattttc
     4981 caataactgt tgttttaaga attgtattaa accagtgaga tagtgagtag ttgctgttca
     5041 caggaagttc ttctgtactt ccgtaccctt tatctggaac aagatctatg taccttttta
     5101 attctgattc attaatatca tctgcaccat atattcgaat attgtatccg ccattggata
     5161 cagaggtaat atcatttgca gaatatactc ttatgtttga taaagagtct ccattaaatg
     5221 tttcccacaa tattctgtct atggatttac caattaacat attcttgctc tcaagtgtgg
     5281 gatctgcagt taaccaaccg taattaggaa tccatatctg tacccactga tgtgtagcag
     5341 gattatctac tatttcctct gagatatttg catatccccc tgcagctcta gcaggtatcc
     5401 cctgagccct aagtagtgca attagggagt ctgaatactc catacataca gaatcgccgc
     5461 ctgttagcgc agctttagca ccaattcgtt cattttctga gtttgccttg ttgtaatcat
     5521 attctagttt atctccaaca aatgcgtagt cggcctttat gaccccaagt attgtacttt
     5581 ggtttgcaat taatttatct gcttcctgtt taatgtatga atcggtactt tgccaatatt
     5641 ttgtaggaag aagatattta tcaaggaaag aacttttctt aacaatgtca aagtattcag
     5701 ttaaattcat atctatagaa tcaacaggag tttcaaaact atcttgctct acaaatatat
     5761 atccagttat ggatatctct gaatcaagat tggcgggtac ttcaaatact cctattatat
     5821 tcccttctgt atcttggtat atgtctttag gcgaggggat tacttcggag aagaagactc
     5881 tctggtttgt ttctccatat tccctaggta gagatatttc gtatatattt gtagaaagtt
     5941 gggaatatat atctgaatat ttttcgggta tgaaggtatc tgttttaggt gtcttaaaaa
     6001 gtagctcaaa tttatatatt gcctctgttc cgaactctag gtacggagaa ttttcaattc
     6061 tatctagttg attgaatgaa tatgttatgg ttttagaatc ttctttcttt gtgaaatctg
     6121 aaggatatat ctttgcaagt ggagagatgt ttttatctgt tactatactt agattgtagt
     6181 tgtatttagt tttagtacca gaagatttgt ctgtctgttc cagaacaata tcttttggta
     6241 gggcaggtcc ttggatagat acaaaatctt ttactttttg tacatagtca tgtgttttgt
     6301 atgtaaaagt tattatgtaa ggcgagtttt tcgtagttgt tttgtaattg ggaatactaa
     6361 tatatattcc attgggatta gcttcttctt ttactgtgta ctttatcttg tttccatact
     6421 ggtcttttac tactatcgaa tcttttttaa attttctctc aacattaatc tcttcatctg
     6481 ttttgttttg tatatctggg atatggaaaa ctttttctcc tgaagcaggg tagtagtaac
     6541 tggaattttc tacggttctt ttgtatgtat attttactgt tacaaaatta tctcctgttg
     6601 tgtaggatat ttgtgtatct gtatctataa caaagtcata tgtggctgcg agtacaggag
     6661 aaatgggaag taaaaagagg agaagagtta aaattgtaag ggtgagtttt ttcatagttt
     6721 taataatatc attttttatc tccctgttat ataatctgcc attatgtcag taaaaaggtc
     6781 acattcattg tctcttggta ttgttggttt accaaattca ggtaagtcaa caatttttaa
     6841 tgcactaaca aaaatgtctg tacctgcaga aaacttccct ttctgtacca tagacaagaa
     6901 tattggggta gtagaaattc cagatgagag actgatgaaa atggcagagc atttcaatgc
     6961 gaaaaaaatt gtccattctg ctatgacatt tgtggatatt gctggattag taaaaggagc
     7021 ctctaaagga gaagggttgg gaaaccagtt tttaggacat atccgtgagg tagatgtaat
     7081 catgtatgtg ttgcgagcat tccaaagtag tgagattgta catgtatatg agagagtaaa
     7141 tccagtagag gattttgaaa ttgtacaggc agagttaata tttaaagata tagaggtaat
     7201 tgaaaagaaa attgcaatgg tagataaatt agcaaggata gggcataagg atgcggagga
     7261 agagttgaag atactcaaga agataatgga agagttaaac aaggggactg cagttataga
     7321 tatggaatta agtgatgatg agaaagaata tatatatgat ctctggttgc ttacaaacaa
     7381 gaaaagaatg ttcatattaa atataaaaga aggaatggaa gaggaacagg taaaggagtg
     7441 ggagagacaa ttaaaagaga aaacaggaga tatggttgta attgcagatg taaaattaat
     7501 agggcagggg gaggaagaaa ttgcagagta tgaagaatta cttggataca aacctaattc
     7561 agtagtggat ataattaaga aggcatatga gtgtttaaat cttataactt tctatactgg
     7621 ttctgaaaag gagtgtaatg catggactat agagaagggt gctaatgtta aggaagcggc
     7681 aggtgttata catacagatt tggaagataa cttcataaca gcagatgtag taaatctaga
     7741 agatatgctt agtgtagggg gatggaatag tgcgaaggag aagggtttgg ttaagaatca
     7801 tggtagagat tatttagtaa atgatggtga ttatataatt atacttgcaa ataaataggt
     7861 atattcaccc tttctattat tgtattaaac aaatttttct gctaaaatcc attccgttga
     7921 taagataatt taaaagtata gataaatggt tgtgaaagaa acaaagaaat taaatggata
     7981 cgaaatggcc gtagtattaa agcctttgtt accagatgat gttagaaaaa gtattcataa
     8041 agaattcgta gatatgattt ccaaaaatgg tggagaagta ttagacgtag atgtatgggg
     8101 taaaagatat cttgcttaca aaattaaagg acataacgaa ggatattaca taatatatac
     8161 ttttaaggta tctccaaaag agattttaga attaaaaaga caaatggaat tgagacaaga
     8221 ggtattaaga tacatggttg taacaataga tgatgttaca ttggcaggaa aaagaataaa
     8281 aaagaaagaa attgagattt aagatatttt taatatcttt ggattaaagt tagaggtaag
     8341 ttaaatttaa tatatataaa taaaatggca gcgagatctt taaataaagt tatccttatt
     8401 ggaaacttaa caagagaccc agagatgaga tatacaaact ctggaactcc agtagtaaca
     8461 tttggaatgg ctacaaataa atcatggaag gatgcaggag gagagacaaa agagtcggca
     8521 gaattccaca atatcgtagc atggaacaaa atggctgaaa tatgtcagca gttacttgct
     8581 aagggtatga aggtatatat agagggttca ctaaatacca gatcatggga aggtgaagat
     8641 ggtgttacaa agtacaagac cgaaattaga gtgaatgata tgattctctt agatagcaaa
     8701 ggtaaacagg gtgcaggtat ggatagtgaa gagggtgcta aggaagagaa accagtcaaa
     8761 gaagaggtag aagaggttgt agaggaggtg gctaaagaag aggaagctcc tgcagaggat
     8821 caggatccac tagaagagga tttaccattt taatttgata gatttaaaaa atgtcagagg
     8881 aagtaataac aaaagtagaa gaaacaacaa cagattcatc ctatggtggt gatcgtaaga
     8941 agaataatag aagaagaaag aagccattgc agaatcttaa atgtcctcta tgtgagtctg
     9001 gtgtaaagag tgttggttac aaggatgtat accagcttaa gaagtttaca tctgtaagag
     9061 gtaagataat ttctactgaa aaatctggag tttgtcataa acatcagaaa cagttaacaa
     9121 actctataaa gagagcaaga tatttggcat tacttccata ctcttcggac gaataatata
     9181 tttgttaaat gaaataggaa aggggaccga aaggtcctct ttttgtttga aaagtactct
     9241 ctttttaagt aatatattta cataatgtct aaactgccaa tactacaaaa aaatgcctac
     9301 ctcagccttg ttgtaaaaaa taacagcata tgggcacacc tcgcatacac agaccataat
     9361 gcaggaaggg agtacatact ctcagatttt acagatttaa atcctcttaa acagagatta
     9421 gatgatgaat tttttactag aaaattttgg tatgaatact tcgacaacct agaaaaggta
     9481 ttcaactggg acattgtgga cagagataca tacagactac aaatgtacaa aacatttaaa
     9541 gatgaaggag taggtttaac tggcataagg gtgttggtgg atgaaaacca gaaatatttt
     9601 aatacaatat tctcttcgtt aagagaattt tccaaagaca ttgctgttag aattgtagat
     9661 gattcataca tctacaacct acttgaagga ttactcggaa gaatggagta tgatgacata
     9721 ctgtatgtag atatggattt gttagatttc aagatataca gaggtaggaa aataatagaa
     9781 aacaaaaaag aaaaaacaat tattactaaa tctaaaatat cttggggaaa tgagtatggt
     9841 gttatagatt cagtgcgaga ttctagattt aaagcattct tagcatcaga ttttgcatca
     9901 aaagatatat taaattactg gagtaacttt gttttaaaca aaatcttttt tacagaagat
     9961 ccaaatatcc tggacatatt gcgatcatat acaactatac agaactactc cttgtatcag
    10021 gataatactg acaaactctc tgggtttgga acagattcta caaacagtgc catgataatc
    10081 tctgggcata taccaacgat acttggcaaa caaaaaacac tcatgactgt agtggatggt
    10141 ttggaaataa tgggagaact agactgtata tgggatttag aagagaaaat cttgtcatat
    10201 ggtaaaagct acatatttgg tacaaactct acagatataa ttctaactag gaaagaaatc
    10261 tttagcccta gtactaaggt gttaattcca gaggtgaaga gtctaaaaaa ccaaaacaaa
    10321 gtcataatga gtggttatac agaatcacta agtatggaaa agaacgaatt ctatgcactc
    10381 tctcctgagt ttacttttat acaattacct aagcatgaag ataagttagt cgtggaaatg
    10441 gaatttaaaa aagacatagt tataccaaat ggtgtaaaaa gtaaggtcgg atttgtttca
    10501 atcccaggca agggagatta cgattcaatt cttgtagatg gaagaccaag accaattgtg
    10561 tatggtcctg atgtatatac aaatagaagt aaattaagta tgtggttgaa tgattttaaa
    10621 gcataaattg tttgaaaaat atgatgaata catattcaaa atcccctccg ggagaggaga
    10681 tagaatattg gtaaatacag gagaaatgtt aaaagagggt acagatatct tggttagaag
    10741 aggaaacaat gtaaaaaatt ctttctttct tccttcacag cttggttgtg caaaggaaaa
    10801 tattgggaaa tatttaatgt gtatggatgg agaatttgtt gaaaaaggag acatacttgc
    10861 agagaaaaca tctatgggtg gtcttacaat taaaagactt acatctccaa tccaaggagt
    10921 tgtagatatg tctagaattg gtattgggta tcttgatctt cttggagaag aacaagaaaa
    10981 cattgtaaag agtacattta atggtgtagt agagagcatc aatccaatag atggaatcac
    11041 aatcaaggcc tctgcatatg ctctagattt ggttgcaata tccaatgtcc aagaagatgc
    11101 taaatatatt gtaggagaat ttgtctcgtt gggaagtgga aatgatgtaa tccttaagag
    11161 tgtagaggaa gatttacagg ataagattgt atttgctggt aaaagacttc atccagattt
    11221 gctgagagat attttcgaaa aaggtgctaa atttgttctc tcatactcaa tggattacca
    11281 aaactttaga aaccaagggc ttcctgtggg agtaataggg ggttttggtg aaatatattc
    11341 accaagggaa atgatagaga agataaccaa aaaagatcat acatttgctg tagtagatgt
    11401 aaatgaaagc caaatattct tcttaggtgg gaatataaag gatggtgttg cagattccct
    11461 gtttgtgcaa agtgcagtag gtaggaaagt catatcccat gcccttggaa attatggaat
    11521 gcttggagag gtaaatggag tagaggacga ttcatattta tctgtgaatt gggaaaatgg
    11581 agcttccagt ataataaatg taggaagtgt agaatttgta tcatattagg gtataatatt
    11641 tttctatgga taaaaacaaa tcattgcaaa aaacattggg tcttgccatt ttactagttt
    11701 gtatatttgt tttgggtttt gtttctggtc gagaatttca aggaaaagat atcttaaatg
    11761 ataacaagga gaaggtagat actactatgt attgggatgt ttgggatatt ttacgctcca
    11821 actatgtaga ttcttcagag atttctcctc aggatatgat gtatggttca attaaaggat
    11881 tggtaaattc ctacaatgat cctgctactg tatttttaac accagaggaa acggaagaat
    11941 ttaaggctac aagtgaaggt aaatatttcc aaggtattgg agcagaatta ggttatgaga
    12001 atggaaacat tattgtagtt acccctttgg atggttctcc tgcaaaagct gctggaatta
    12061 gggctggaga ctatattctc tctgtagatg acaaagatat aacggcagag gatacggtat
    12121 atgatgttgt agcaagaatt cgaggagagg aaggaacaaa ggttaagctt aagatattgc
    12181 ataaagattc gaacaagcca gttgaggtta gtatagagag gaagcagata actgtggcaa
    12241 gtatgaatgt agaattttta gaaaacaaga gcattgcgct tttccgagtg aataggttta
    12301 cggaagcttc gtactcagaa tgggtaaaag aatggaatac aaaagtaaaa gagataaaag
    12361 atgcaaaagt taataaggtt attttagatc ttagaggtaa tccgggagga ttctttgatg
    12421 cagcaatata tgctgctgat gatttcctgc agggtggata cacaatttca atgcaacagg
    12481 atggtaaagg gaaaatagat aaatacgaat caaaagatgg tggaaattta acagaagtaa
    12541 aactagtggt gcttgtagat ggaggaagtg catctgccgc ggagattctt gcaggagccc
    12601 ttcagcaggc agatagggct accattgttg gagaaaagac atatggtaaa ggaacagccc
    12661 agaatgtaat agatttaagt gatggttcaa gtttgcacat tactactttg aaatggttgc
    12721 ttccaaatgg aaattggtta aacaaggagg agccaataac tccagatgtt ataattgaat
    12781 ttacagatga agattttatt gcaggaaaag atcctcaatt agtagaggct attaagcaaa
    12841 taaataaatt gtaagatatt taattatgaa aaaatttaaa gagatatttt caaaaaaagt
    12901 tttgtatatt attggatttg tacttgcaat tgttatgatc ttgcttattg gtggatatct
    12961 tctgctaaga aatgcagaga gtattaaaaa ctctttttca tttcttttca atagtgaaaa
    13021 tacttgtcaa gaaagcgata aattaatagt ttctgagagt gaggcaaata caattaagat
    13081 agttaatgag tctcaagctt ctgttgttag tatagctgtt tcaaccctct cagtttctaa
    13141 aacagaggga attgttgaca gtagcagtaa tattgggaca ggttttatca tagattcaag
    13201 tggacttata cttacaaatc aacatgtggt atctagtgat actgctgtgt accaggtaat
    13261 tacaaatgat gagaaaaaat acgatatagt agaaattgtt cgagatgatg taaatgatat
    13321 tgctttaata aagattgatg caaaagattt aacaccaatt aagctaggta attcagatgt
    13381 attagctgta ggacaaaaag tactcgctat tggaactcct ctgggtaaat atgcaggaag
    13441 tgctactgag ggaataataa gtgggctgga tagaaccgtg accgcaagct ctgactggac
    13501 tggatttaca tccaagacat acgaagatgt aatacaaacg gatgctgcag ttaatcctgg
    13561 aaactcaggt ggccctttga ttaattcgca aggagaggta attggaatta actttgctac
    13621 tacatctggt gcagataata tctcttttgc tattccgata aacaaggtga aagagagagt
    13681 ggaggagtat aagacatacg ggaaatttat aaaaccatat atgggaatct cttatgaaat
    13741 gttctataaa tatgatcttc tttattacac aaatattgtc cctggagctt tagtaaaagg
    13801 attggataca acaggaccag catacaaagc tggaatcaga agaggggatg taatcaccaa
    13861 gtttggtgga gaggatgtaa atatagcatt aataaatcta atacagaaac ataaagttgg
    13921 tgagagtgta aaggtagagg tatatagaaa tggtgataca aagagttttg atgtagtact
    13981 aggagaagcc aaatagtaag aatctattaa gtcctctaaa atcctgtatt accttaatag
    14041 gttcttttat atttgtaata tacttaattg tagatggctc tatctccaca attaaaagat
    14101 tgttaccttt tggtatctgc acaaaaagtg ttctgtaata tttcaaacca tcctctcctg
    14161 cttccaaggc aagttttggc tcgaaatcgt gtacactgct atctaatgta ttgtacatat
    14221 cagttggaat atatggtaga tttgcaatat acagagtaga gggttcaaac tccatccctt
    14281 taagtaaatt attctgttta aaaattatct tttcttttac attgttcagt tttgcattat
    14341 ctctggctac atctaatgca tttttactta tatctatagc gtaaattgag atattattgt
    14401 ttaattcttt cgcaagtgaa attgcaatac aaccagaacc agttccaata tctactatgt
    14461 tttttatacc ttttaaattc tctttcgcaa tatctacaat ctgttctgtt tcaacgcgtg
    14521 ggataaggac attactattg acattgaatt tgtagcccct aaattcggcc tcaccagata
    14581 tatattccca tggttcattg gatagaagtc tattgaaaat ctcttcaggt ggaattttat
    14641 tagatcttat ataggtgaga atctcttgag atattcttgt tatatcatgg tagccagaat
    14701 cttttaaaac agattgaatt ttgtaaagta gggctaaatc gttattcact cttttctaat
    14761 gcttgcaatt gaataccctc tctaacatca tcaattattg tttgaatatc tccgtttaag
    14821 attgattcta gattaaacca agatttcttt atcctatggt cagttactct gttctgaggg
    14881 aaattgtatg ttctaatttt ttctgccctc atggaagaac caatcatgga agaacgaaga
    14941 ttagatcttt ttgcatcttc ttctgctttc tttttgtcat acaatcttga tctaagtaaa
    15001 gccattgctt tctctttgtt ttgagcttga tgctttgttt cttggcagga aacgacaata
    15061 cctgttggga ggtgtgtaat tctaactgca gaatctgtcc tgttcacgca ctgtccacca
    15121 gcaccagatg ctctcattac ttcaagctta atatcgtcag gattaatttg gatgtcaata
    15181 tcttttgctt caggaagtat agctacagaa gctgtagagg tatgtattct tccagatgat
    15241 tcggtgattg ggattctttg tactctgtga acaccacttt cgtatttgag atttttaaat
    15301 acattctttc cctctatatg tactatgact tctttgtagc caccatcctc tgttatgtta
    15361 ctgtctataa cttggatatt ccagcctttg tttatgctgt agtttttgta cattctaaaa
    15421 atatctgcgg caaaaagaga agcctcatca ccaccagctc cggctcttat ctcgagtatt
    15481 gcagatctgt tatcatcttc atcttcaaac tctcgtgcaa ttttgagctt ctgtattgct
    15541 aattcaagtt tttctaattc ctgctctttg ttttctatct cttcagtagc catctctttt
    15601 atgaatgggt cattttcatc ctcgagtaat tcttgtgcat cgttaatctc ttttagacac
    15661 ctttgtattt tagagatgag agaggaaagc tcggagtagt aggagaggtc gttgcttaac
    15721 tgcgttatat ttgttccact attgtgactg gaagatatct gattctgtat ctcaatcacc
    15781 tttgtctgtg catcattggt ggaatatttt gtttgtaagg aagttatatc cataatggat
    15841 aatatatcat gtaaaaatat ttattgaaac ttgttattta gaaacttttg cgttctctag
    15901 catatctcta agtgtaagag atgaagaagg tctggtttct gccattttct tttttcttgc
    15961 agccattttt tccttcttac ttctgaaaga catacccttt gctttttctc ttctgtcttc
    16021 gaactttttg acaaggttgt ctgaatcgac aatcttttgc tctccagtgt agaaaggatg
    16081 acatttggaa catatctcaa cagttaaact gtcagattca agtgtgcttc ctgtaacaat
    16141 cttgttacca catgagcatg tgatttctaa actgtcatta tattttgggt gtatatcttt
    16201 tttcattgca gggagattat agcagaagaa tattctggtg tctagaaaaa cacagggtgg
    16261 ttgataactg gaaactaatc aattatactt tccatatgaa gtattcgaga ggaattgaaa
    16321 acaggataaa aagagtagaa ggacaagtag gaggtgttag gaagatgatt atggctcagg
    16381 aaggtgatac taaagttatg actcagctcc aagccgcaat ctcctctttg gagagcttga
    16441 agacagagtt aattaaacag cagatgaaag agtctatggt aggagatatt aagaaatctc
    16501 tcggactctc agattaagta tttcttcaaa ctgcccctga agaaatctgt tatactaatg
    16561 aagtatttgt taattttaga catagtaaac tatgaagatt aaaagagtcg gatggaacag
    16621 tttcctaatt tcttcatcca acatttccct aattactgat ccattggatc ttttgcaatc
    16681 tggagtaaca tttcctaaaa caaaagcaga tatagtactg ttttcaaatt cagatgagaa
    16741 cagtaaggac agtattctca aggaaaaaaa gttagaaagt aaggtagttc ctgacacaag
    16801 agaaacaatt atggagatat ttactcctgg agagtatgag gtaggggggc tgatgattag
    16861 aaggagtatt ggagagaatt tttacatgat tgacgaaaaa aatatcagag ttttgtatct
    16921 tggtggtaca gataacagct ttaatcctga tagtgtaaaa gatgtcggtg atgtagatgt
    16981 tttaatcctt ccagtaggag acggggtaaa ttttatggat tttgagaaac ttgaaaaagt
    17041 aatttctaat atagatccat caattattct tccatgtgca tacaaggaag agggttcaaa
    17101 atttgagggt ataaaaagca aagatgaatt tattaaatac tttggatttg ccaatgtgac
    17161 tgaagataca acgctcaatg tagctaagaa aaaagttgaa gaagagcaac agagtgtaga
    17221 agtaatattt ttgaaatagt agatatggaa gaaaaagttc caccacaaaa cacagaagcg
    17281 gagcaatcac ttcttgggtg tttgctttta gataaagacg cagtaatagc tgtttctggt
    17341 tggctaattc cagaacattt ctacgatgaa aggaatgcca ttgtatattc taatattctt
    17401 gaacttttta atgacgctat cccagtagat cttgttactc tctctgaccg tttgaaaaaa
    17461 aagaaaatgc tctcaaagat aggtggtaga acatatcttg cagatctttc ttctacagtt
    17521 tccacatctt cacatgcaga ggaatacgca aatattatta aggaaagtgc aacaaggaga
    17581 ggattaatat ctgctgcaaa agaggttaca cagtatgcct ttgatgaaac tcttggaatg
    17641 gatgaggtaa tagacagatc tgaaagacag atttttgata tagcccaaaa aggtactaga
    17701 acgaactttg ttcatataaa agatcttctt aaagatgcat acgaaagagc agaaagagca
    17761 gatagagatg aagcatatct tggaatatct acaggtttta aagatctaga tgatttatta
    17821 ggtggatttc agaaatcgga ccttattatt cttgcggcaa gaccatctgt tggtaaaaca
    17881 tctctggcat tagatatgat gagacatgct gcaatggttg aaaagaaaac cgtagcattc
    17941 ttctctttgg aaatgtctca aacacaaata atggacagac ttttgggaat gcaatcggga
    18001 attccattct gggaaattag aacaggaaga ctgagtgaca agaaatttat gaaattggcg
    18061 gacacaatgg gagagttagc agatgcaaac ctttacattg atgatcaagc aggacagcat
    18121 attaatgcaa ttagaactaa agccagaaga ttagttcttg aaaagggact agacataatt
    18181 tttgtggact accttcagct tatgcatggt agtaaccaag agagtcgtac tctcgaagtg
    18241 ggggaaatat ctcaaggatt aaaaaatatt gcaaaagaat taaatgttcc tgttgttgct
    18301 ctttcccagc tttctcgagc cattgaacag agacagggta ggagaccaca gctatccgat
    18361 cttagagaat ctggttccat cgaacaggac gcagatgttg taatatttat agatagggaa
    18421 gaaacatgga atccggatac agaaaataag ggaattgccg atttaattgt agctaaacat
    18481 agaaatggac caactggcct tgtaaaattg gcctttataa acgagattgc aagctttagg
    18541 aacttgtata aaggcgataa atagcgatat aatacagttt aacaaggtcg agtggtggaa
    18601 ctggcagaca cgcgggactc aaaatcccgt actagcaata gtatgagggt tcgattccct
    18661 cctcgaccat atttttaatt atattaagca aaatatggaa gattttatct taggtgtttt
    18721 agaaggagcg gggagtatcg atttcaatat agtctggaag atattactct ttgcatttgt
    18781 aatattctgg attgtggttt tagactggat atgggcagac tctggggaaa gaacatcgag
    18841 caagagaagt agaatattgt acgtaatatt ggcggcagtt ttcaatgtct ttggttggtt
    18901 aatatattta atcattagac caagtcaaac gatagaagaa atatactggg cagatttgga
    18961 gagaagatat cttaaatatg agacatccga attaggagat tgtccaaagt gtggaactca
    19021 actttatcca ggatatcttt actgtccaag ttgtaattat aaacttaaaa gaaagtgtcc
    19081 atcatgtgga gtgtatgtag ataaaaaatc taacttctgt ccattttgta gtgctgtaat
    19141 taaaaccgca gttatagaaa aagagatagt tactccaagc atagaggaaa tgcaaaagca
    19201 gaatcttgaa gtcagagaag aacttacaga ggttgtagaa actcaaggga ccaaatacag
    19261 tggaggaaga agaagtgtga ctgcaaaatt ggggggaaag atagttggag gatacaaaat
    19321 tatagcatca agtatagtga aggtatttaa gaaaaaagaa gctaagaaag agatacaagt
    19381 acaaccaaaa gttaagagga ataaaaagaa acactcttca aaaaagaaga agcagaagag
    19441 ataaacgtgt ggtatcatat atactgtgat taatacatac attgaaaagg caataaattt
    19501 tctttctcag ataaactctt cacttgtctt tatatttatc tttcttatgg gactaataat
    19561 tttctggaga ggaagtgtag agagtagaaa ggaaaggtcc tctacttttg atttgttttt
    19621 tctttctgga ttctttagtc tagtatctgc tcgtattgca cacattgttg ctaattggga
    19681 ggctttctct ggatttatat ggtattggtt accctacgag aggtatgggg aagagatatt
    19741 cctgtttaga cttcttccat ggagattcct aaatatcttt gatggaggaa ttaatattct
    19801 tgttatgttt gtaagttttc tgctctttgc aacgataggg gtagtcataa ttaaaaaatg
    19861 gaaatggcga gatatgtttc cggttgtttt tttctctgca gaatctatgc ttgctatgtc
    19921 atttcttttc tctggtgtca tagcgaataa tatgggatgg atagtacagg gaggagtctt
    19981 gctaattccg attatcttaa tattaatcat tctctctttt gtaactccaa acacagaagg
    20041 caaggaagaa aaatcgctat acataattgt aaatattttt cttatacttc tttcaactgg
    20101 atttattctt tatgtttatc tgaaaaacga cattgaatta atagacaaga ttagctgttt
    20161 aatatttcta atctggagct taataggtag cctatctttc ttgagggaag aaagaggatc
    20221 tagattttcc attgagagag tatcttctgt tagaacaatt tcttctgcag atattaatca
    20281 accaatcaga ttatctaaat aaaatatatg attgtaacca tagggagaga cataacaagc
    20341 ggtgttagag tcgacatctt tgttctagag caattgaaat taatgggagt tgaagtccca
    20401 agtagatctt ttcttcataa aatgtttaag gaagttatta aagtaaatgg taaagaagta
    20461 aaatcttcct acaaattgaa aaatggagat gttgtaaata tctctgaaga aaagattgaa
    20521 caagaattaa acaaacagga caagaatatt ggtataactg cacaagaggg aaagctagat
    20581 ataatacacg aaacagagca gtatcttgtc ataaataagc ctaagggtat tactgttcac
    20641 ccagggatta agaatacaga aaatactctt gtgaattttg ttcggggata tcttgaaaag
    20701 aaaggggagt ttaatccaag gcttaatagg gcaggacttg ttcatagatt ggacaaggga
    20761 gtttctggca taatggtatt tgctaaaaca gttgaatctc aagaatatct acaaaaagaa
    20821 tttgaaaacc atacggtaaa aaaaatatat cttgcaaaaa cagaaggaag ctctgagctg
    20881 accatataca accgaaatat caaagaggag ttagataccc ttctaaagag aaatttcgag
    20941 atggacagta cttggaccct ttatgaggga tatataggta gagataggag aaacagatta
    21001 aggatgaaat ttagagagga gaaattccct aattcaaaga aagcgatatc ttttgtaaaa
    21061 agaattggag agaatgaatt tcttgtatca attgaaacag gtagaatgca tcaaataaga
    21121 gctacacttg catatatagg tatcccaata cagggagata ctctttatag aaaaacgaaa
    21181 aaagatggta tccccgaaga aattgaactt gaatcgatat taatatcctt taaagatatg
    21241 gatggaaaaa cagtaacttt cagactctac taaatgaaaa ggagaagaac aaaaaaaaca
    21301 aaaaatataa atatcccttt tagatttata tttttattga tcgtggcaac actttttctt
    21361 gggtttgtaa tatactacat ctttacagta acaagcataa aaagtataga taaaacaatt
    21421 cttaatggtt ctcaaagata tcttctatca caaagcaatg gtgagtccaa aaggacagta
    21481 atcatccttg aggaaggtta cggaaatgag agaagaatat ctgaggtata tcttttttta
    21541 agtaatcaag aaaaaggcaa atcacttctg gtatatatcc caagctggtt gtattatact
    21601 ggactagaag agaattttgg aagtgcaatc ccaatctcct cttttagata tgctggagat
    21661 acaattcaag aggatagggg ggttgagtat tcggtttggc agattagtca gattttgggt
    21721 attacattta ataactacat atatattagc agtgaagcaa acagcacctt ggattcagta
    21781 ttcggagaag atgtcatggt aaaagacagg tatagagata tgtttaaggt aggagataaa
    21841 gaatctcttt ccgaaggttt ctttagactt caagcacttt cttcgaagat atcttttgta
    21901 aaaagtctgt ttaaagtaaa tgagtttaga aattttagta acaacatcta ttcaaatctg
    21961 tcctttatag agggtataac tatgctaaaa aatgtcgaga aaacactaca agcgacagag
    22021 tcatatgcaa ttgatctttc agacgcaaaa tattcgaagg aggagctctc cgattctgga
    22081 ggtataaaaa gatatttttt aagtaaggaa ttcgacaatt catacagaga ccttatttct
    22141 ttacttatag acagagagct cgagaaggag agagtccgag tagaggtgta caatggcagt
    22201 ggcgtagcag gagctgctat gcaatttggg agaaaaattg agaattcagg ttgtgatgta
    22261 gttaggtatg aaaatgctcc agacattatc gataaaacga ttatgtatat tcctaagcta
    22321 gatgatttta agaaatcatt taagatagtc tcagagatat tctcagataa ttttgagctt
    22381 gtggaaggac gaccggattt tatgaccaca ggagatattg taataatcct aggagaagat
    22441 attaaaagaa tgtacagttt ctagggtatt tgcgtaaact aggtatttaa tctaaaattg
    22501 ctgttatgat aactgcatta ctcttattaa tacttttctc gttagtattt actgcttttg
    22561 tactaatttt cgcaaaaaga aacagagaag aggagataga aatatacgaa gatatccgaa
    22621 atcctcttgt aatgcagata ctggtcccaa gagaaaatga taaatcagca cttgcagcag
    22681 agcagatgtt tgcttccata cacggtattt tgggagattc aaaaaaatct ttagatttaa
    22741 ttagttttga aatcatatcc agtggagaag atggtattag attctttgtc gtgacaccag
    22801 gtcatttagc aaagtttata gagggacaaa tctatgcaca atatccaaat gcaaatatta
    22861 cttacgaaaa agattattcc ttgaacaatt cagaattagg tgaagaggta tatgtcacaa
    22921 gtggagaggt agagatggag aaagattcca tatttccaat taaaacattc agaaattttg
    22981 aagtagatcc tcttgccgct ataactgggg ctatgtctga gatgggtaat ggggaaaggg
    23041 cttggcttca aataattgta agaccagttt ccaattattg gcagaagaaa tcaaaggaat
    23101 atatctctgc agttagggaa ggtaaggata ttggaggttt caacattatt gcaaaattca
    23161 gaagtttaat ctcaacaata ggaaaaaatc tcacatactc tgattctcag gcagccaaaa
    23221 aagaggttgt taaattagaa cctgggcagg aagaggaatt aagtgagatt gagaccaaga
    23281 tgcttaaact aggttttgag gtaaagatta gaattgttac taaagctaca acacaggaga
    23341 ggtccgaaca gattctgaga gatattgtgg ctagttttaa acagtttact actgcacatc
    23401 taaataattt tattcattct gtaccggtaa aaacaggcaa agaaatttat gaggaatatc
    23461 taagaagata tatgccaaag gaagaggagg atatattaaa cattgaagag cttgcgtctt
    23521 tgtatcatct tccaaatatt tcagtagaga caccaaacat agcgtggagt agatctaaaa
    23581 aacttgaacc tcctatgaat cttccaaagg caaatgatgg agatgtaaca atatttggag
    23641 aaacagatta cagagggcac aaggttaact ttggattaaa gagaggtgac cgtgctaggc
    23701 acttctacct tcttggtaaa acaggtgctg gtaaatctac gctttttaaa aacatgttca
    23761 tatctgatgt actggcggga gatggagcat gttttattga tgcccatgga gatactgtag
    23821 aggagctttt ggagtacata ccacctaaca gaatagacga tgttatatat ttcaatccta
    23881 cagatattga aaacccagtt ggattcaatc ttttggaatt aaaagacaaa tctcaaagag
    23941 atctaattgc agatggtgta gtagaggtgt ttaaaaagca attcggtgat tcatggggcc
    24001 caagacttca atatattctc accaataccg tagctacagc tcttgaggca caaggtataa
    24061 ctctgttagc tgttattaga atgttgacag atataaatta ccgtaaattc atacttaaac
    24121 aggtacacga cccaatactt tacaagttct gggtagagga gtttgcacag atgtctcaaa
    24181 atccaagact tcttgcagag gctgtatctc caatccaaaa caaggttggt agattcatat
    24241 cttctgctgt taccagaaat attgtaggtc aggtcaaatc cactatagat ttaagagaga
    24301 ttatggataa tcgtaaaata cttcttgtta atcttgcaca aggtaagctt ggagaagaaa
    24361 cagcatcact ccttgggggc atgataatta cgagacttca ggctacagct atggaaagag
    24421 tagatatacc gtttgaagag agaaaggatt tcttcttgta tgtagatgag tttcaaaatt
    24481 ttgctacgga ttcttttgca aagattctct ctgaagcaag aaaatacaaa ctcaacttaa
    24541 ttatgactaa ccagtatatt gatcaattac ctttaacggt aagacaagct atatttggta
    24601 atgttggaac attgggttca tttgtagtga gtcaggcaga tgcttcaatc cttgagaagg
    24661 aatttgcccc tgtggtatct gcagatgact tggtatcttt ggatgcattt tctatgtatg
    24721 taaagctctg tatagatgga atgacctcta caccatttag tgcaaaatct cttcctctaa
    24781 gatatgagaa gttccactta aaagatgaga tagtgcagaa atctagagag aagtatggtg
    24841 tacctagagc agagatagag gagaagatag ataagtggag taggcagaca tatagtgaga
    24901 agggaaatcg atcaataaac aaggaaaaag aagaaagcga tataaggcct gtagcagata
    24961 gaaaatatga aaagaaagaa aatgatatcg aggagaaagt atataaagta aaaatttaac
    25021 aatatgtcag gacattctaa gtgggatacg attaaacata agaaagcaat aaatgatgca
    25081 aagaagggga aggtattttc taagctttcc atgatgataa ctcatgcatc taggcagggt
    25141 ggtggagatc cagagatgaa ccctaattta cgattgtata tagataaagc aaaagaagca
    25201 ggttttccag cagacagaat agaaaaggca atccaaaaag gtactggaga gggaggggat
    25261 ggagttgtat ttgaagagaa tacctacgag ggatttggtc ctttcggaat tcagatgctt
    25321 gtagatactc ttagtgataa caagaacaga acagttgcag agattagaca actttttgat
    25381 gatatcggag gtagaatggg ggacacaggc tctgtgtcgt ggaattttga agctaaggga
    25441 tacattgtaa taaagagtgg acatatgatt aaatctccca aatttggaga agaagatatc
    25501 ttcgaaaagg aagaatcaga agcagtgatg ctttctatca tggatattga aggtgtgttg
    25561 gatatagtgg agatagaggt ggatggagag aagggatttg agatatatac agaatttcat
    25621 caatttgcac atgttagaga tgctatatcc aaattaggat atgtaattaa ggaagctatc
    25681 ataataaaag aggctaagat aaaaaaagaa ttaaaaccag aagaggtagc caaggtggaa
    25741 gaagctatag agaagttaga agagtatgat gatgtccaaa atgtgtggac tgatttagta
    25801 gaataatata atgagaatat taggaattga tccaggactt gctactactg gatgggctat
    25861 agtagatttc aatgataatg gagatccaac tcctgtagat tatggtgctt taattactaa
    25921 aaaaggctta actgtttctc aaagaatagg agagatatac acagatttac atgaacttat
    25981 caaaaaatac aaaccagaat acgctggtgt agaaacatta ctcttttgta aaaatgtcac
    26041 aaccgctatc tctgttggag aggctagggg agtagtactc cttgtcttag agcagaacag
    26101 gatacctcta aaagaattta ccccattaca ggtaaaaaac tctatagctg gatacggtaa
    26161 agctacaaag agacaggtac aagaaaatgt aaagatgata tgtaaattaa aagaaatccc
    26221 cagaccagat gatgcagcag acgccatagc tattgctata gctacacaag ttttaatatc
    26281 aaggatataa taaataatga tttcatatat atcaggtgtg gtccaaaaaa tatcttttgg
    26341 taaagattcg tacttagatc ttcttaccaa gatggatatc tcttaccgag tgcatattcc
    26401 tcaaaatttt gctcctattg agaaaggtca acagctatca ctgtatacac atttccatgt
    26461 tagagaagac attcaatccc tctttggttt tgaaacagaa gaagaacggg atttttttga
    26521 agaattaata acagtttctg gagttggtcc taagatagca cttgcagtac tttcaactta
    26581 caagaaggat gatttggaaa agataatact tgaaggagat gctaagaagc taagtaaggt
    26641 ctccggacta ggtagcaaag gagctcagaa aataattctg gagttgagag gaaagataga
    26701 ttttgacaaa gacggaaagg tatctagtgc aaagagtaga gacttaaaga atgctcttaa
    26761 ggttcttggc tattctggag agatgttaaa tgagaaagta gatttaggag agaagatttt
    26821 ggataaggat gaaagtatag agatagaaga tttaattaag agggtactat ctgcataatg
    26881 atacaatcaa gttttgttag tgaaaatatt gagaaaatag agaaagcacc taagaaaaaa
    26941 gatgctcaat tagagcaaaa gattagacct agtagattag aggagattat aggaagaaaa
    27001 caggagaaga gaactcttct gatgatgatt gaatctgcca agaagagaaa agaagtgata
    27061 gatcatatcc tcttctatgg acctccagga ttaggaaaaa caacattcgc aatggccatt
    27121 gctaatgaga tagggtgtaa tattaaaata acctctggcc cagcagtaga aagacagggg
    27181 gacttagcgg ccatattaac tggcttgaaa gcaaatgatg ttctctttat agatgaaata
    27241 cacagattat caagggtagt ggaggaaatt ctttacccgg ctatggagga tggagtttta
    27301 gatatagtaa tgggaaaggg tccttctgca aaaaccatac gattgaactt agagccattc
    27361 acattgatag gggcaaccac ccagataggg aagattagct ctccaatgag agaaagattt
    27421 gggttagtgc agagattaga tttttttagt gacgaggata tggtttcaat tctccgaaga
    27481 gcggcaaaac tttggaatat taaaatagag gagggggctt tggaaaaact tgctagatca
    27541 tcaagaggta ctgctagaat aggattgaga ttactcaaaa gaacgagaga ttatgcagag
    27601 agtgagaatg taaaaacaat tacagagaag atagtaggag atactcagaa acttcttggt
    27661 atagatgaaa gaggtttgga ggagatagat agagagatat taaaatgtat aatgctaagc
    27721 tttaatggtg gaccagtagg tctttcaaca ttggcagcct ctatctcaga ggatgtaacg
    27781 acactttccg aggtatatga gccatatttg atgaaatgtg gtttaataaa aagaacctca
    27841 agaggtaggg tcttaacaga gaaaggttta aaatatatag agggaataat atatggaaag
    27901 agctaatgta ggctcagatg tagaaaaaag aggaaagctt cttttgcttg ctggattaat
    27961 agccgtggct atgtttttga acaaaatatt acagaaagaa gcatctgttg aaattcctaa
    28021 tatatatctt aatggggctc tgtattttgc tatatcattt gttggccttt tatggtcttt
    28081 caattttcaa attaaatcta aatccatcct ctatattatt caagcagcac tttttgtatt
    28141 ttctgaagcc atatttatag agtttttttt ctttcaaaaa tttaatagat tgtatgaagc
    28201 tcttatcctt ctaattcttc taacgcttgt ctttattggt aactatgttg cctttcttat
    28261 ggctaatgta tttaatgttg gtcttttcaa aaacatccca ttggtacatg taggaaggac
    28321 ctcgtcatat cttctttcac tactcaccat ctattttgta actttcagtt ttctttctag
    28381 taacttacca atatatattc ttttaccttt gattgttatc gcttatgcaa tcgttgtgta
    28441 tattcactat ttaaatatcg gaataggggg aggtaatcta atatggaaga cggtactaac
    28501 aggacttgtt gttttagttc ttttcttggg agttttctta actggaaatt ctcacgaaat
    28561 catctctttc gttcctgtag tagggtactt cttgtgtgtt agtgtagtaa gccagagtga
    28621 cattgagtat aaagaacgaa atgtattttt ctatctgatt tctttagcga tatttttctt
    28681 tattgcagtt tttataaatc ttctcagtta gaaacaaccc atatgtttca tagagtaggg
    28741 ggtggaggct aaaaggcctg tagtgtgcga taacccatag taatatcatt gtggataact
    28801 gtttgctaat tctttccaga aaaaaataat tgatgtaata aaatagggaa taagaatcta
    28861 aagattctgg aataactaat tgtttataca gatgtagttg atacccttcc cctatgaaga
    28921 atagtatgtt acaatttgat tacaggtaac cgagtataat gctccttata tgcggtgacg
    28981 gtattgtaag aagcgaagag tcggttcctg tagcttattt taaacacttg gtaatgatgc
    29041 ccagatatgt ctgtattctc tttttaataa ttcattccct ttcttgttat ttctcaattt
    29101 ctataccttt taaattttac tacccctacc ctgattactt aaattttaat ttgtagtaac
    29161 atctgattat gaaagataca ttcccaaagc taccagatca ggacgatttc cttcttaatc
    29221 tgcaaaacag taattacagt atgcaaacga tatataacta tgcacgagat ctttgtatat
    29281 ttgctgtata tctccatttc ataaatgtag atttcaagaa tattaccaaa agagacatat
    29341 caaactacaa gggataccta agagctggtg aacaccttaa ggatttagat aaaattagga
    29401 gtaaagtgtt aaataatgtt gatataccaa cagaaagtaa gggtgaagct cctagggacg
    29461 atttaagggt cggtaatgac ttgggtggtg tgaatacacc tctaagtggc tctgaaaaag
    29521 agaaaaaaga cacaggagtc tttgaaacgg attttttggg agaggtttac agtaaggttt
    29581 acggtggtct tggaatttat ggaaaaccaa tgaatg
//
LOCUS       DAOQWP010000008        26814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_2114_length_26814_cov_7.408760, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000008 DAOQWP010000000
VERSION     DAOQWP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 26814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26814
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_26814_cov_7.408760"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1438)
                     /locus_tag="PKI16_01650"
     CDS             complement(29..1438)
                     /locus_tag="PKI16_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53162.1"
                     /translation="MSKKNIFLLVFSIIIFSVLAFLLFGNNKKTDQIVYEDTLTISKE
                     YLVLRYKTDNVLVNAKDYESYDDWNEEIASIIQEWETLESKALKLEENAEEMSNEATA
                     FNIIKSAYAKDYSYAEVQAIVEKAPAGRQIRTLAQHLGVDAKRAQLILNQSQDMATRE
                     AWGEAGDTFEKLENQAIVVKDTCKVTVYVGGIVATGGGAGIVSQVTTVVVGVDLALEV
                     TEDSAQIALGDRNKVSSFVKDVRTVTEPIASVLTITNIPSNLGSAYGKFDSVMVGLEQ
                     FRDTVQEGKVIGIDLKNFEYHPPFQVIKNTQYPGDVTVAEMEMAEVEEWIKSINKDYK
                     PMTEEEVQEFLGITKKETEPTEAVAKKEVAKNEKENNVEQTEGKKDEISESPRNEIKL
                     MPVEEFEELFSDFKIENISEVKEIFGEPDVVNTFDDGYGNYIYHRRLVYPNGNIAGMM
                     IRFQNLDVTGYSIMMEEYQ"
     gene            1740..2327
                     /locus_tag="PKI16_01655"
     CDS             1740..2327
                     /locus_tag="PKI16_01655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNR53163.1"
                     /translation="MKYKICQMQENDISEVIDFHNKYLGTRDFINKKEISNRLKSNNG
                     IFFVAKDEKGKIIGIKLGYIENDTCIGRGIAVDKRYRRLGIGKELVKNFEDRLKSFPS
                     VKKYIFASSTQEGIPFHIKLGYKPSVLLQSNNKELLESINLEEFTLKGKSYNKVYNVH
                     QIYLEPDQEPDLSYLSQIKSRCSGIDIQYLFEKDF"
     gene            2627..3541
                     /locus_tag="PKI16_01660"
     CDS             2627..3541
                     /locus_tag="PKI16_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53164.1"
                     /translation="MKITWTPSSSSSTQTPYTTSPTPYKTYQTFTLGKDTPITVLQSL
                     HTRSLLITKDNKPARVYLGMARAKDLCIATYTIASTKEFKNAINHKYIPEKNAISVML
                     IREPLAQSPFINTYMGSGFESRLYAVMDVHHVKDVKGLKGISGRVCSYKIDIPESKSR
                     EIHEDIRLAIIYDSIAGGRNIIASIRELKKQFKNLKKVVLFSVYSTYQGSKRIANICK
                     KNKLACEFFCMHELLNASPLNEYDCFYPSWNISKEDEDILKSFYGEKYNKICIGGDWT
                     ANTLGKEQAVDVFKSQAKELGIDTSRYF"
     gene            3556..5298
                     /locus_tag="PKI16_01665"
     CDS             3556..5298
                     /locus_tag="PKI16_01665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNR53165.1"
                     /translation="MSKKKEKSFLNIFLFVFLFLFGLELTFAIGYLLKSPLQKTISSI
                     PFTSFTFDTSKLTYQQPEHTEITPLSSLTILPKEYTSFTKQYGTLTQHEKTELLRYST
                     TTHRTESKTLTELYADIYNNNLNILIPSQYLDIYFLTQFQDIANRIKEEDTFPSTSQE
                     LSRIFQNGITLYNDSEVEDITNVSDFSILSKWLLTQKDITEENTYTAFLDNIYNKYIT
                     SEKSTETLATEILNEEYIGLIYFDTNPLQLSKIDYILQFLAEVYTQAKLDNKEILYQI
                     NTLKQYSQDLKDSNTADIPLEEILTNPNLNISAPEEPVSLLIKTFEDKTYLAPTYTFS
                     KDFVVNDKNFDEPYDVKPIAQSKGSTRVPIFMYHQIGNPPKGASKFIAGLYITPQQFE
                     EQMAYLVKNNYKTIGSLELYNLLKKGGNPSQKTVMLTFDDGTYGQYQYAFPILKKYGL
                     TGTFYVISNRSSIGKTSILKEMAQAGMEIASHSATHPNFVNLKDLDQLTYEIYSSKSM
                     LAARTGKPVYGIAYPGCVADSRVFSRVAGAGYLIGGSCGKSIDHYLSKRLSLSRVHAY
                     GDMKSFKKLLSGAY"
     gene            5316..5963
                     /locus_tag="PKI16_01670"
     CDS             5316..5963
                     /locus_tag="PKI16_01670"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNR53166.1"
                     /translation="MKNDFSSLKELHEYYAVNNNCKLKNIATQPVYEITPPSSGIVFI
                     GEAPGKNEDLQGKPFVGAAGKFLDQLLKGIGFTRNDVYVSNTVKYRPPENRDPTDLEK
                     DSCRVWLNAELLFIQPKVIVPLGKHALARFVPDTAITVVHGNPYSHPSGIPIFPMYHP
                     AAALYNPNLREVLQKDFEKLKEFLNKGGKLLENSIEDSSGFSKQSKQDIDELLKL"
     gene            complement(5965..6573)
                     /locus_tag="PKI16_01675"
     CDS             complement(5965..6573)
                     /locus_tag="PKI16_01675"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNR53167.1"
                     /translation="MNRPSIAEERTLILMKPDVLQRQIVGEILSRFERKGFKLVAMKM
                     VNATKEQVGEHYTGDEQYLIETGEKAKKGAQMRGQDVSNWNSLEWGKIIRERNIAYLT
                     CGPILAVVLEGPGVVAGVRKVLGSTSPADGDVGTIRADYSFDSYALADVVARSTRTML
                     HASDSPENAKREIKIWFKEKEICNNYETVAEKIFYDAGWDNV"
     gene            6621..8252
                     /locus_tag="PKI16_01680"
     CDS             6621..8252
                     /locus_tag="PKI16_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNR53168.1"
                     /translation="MEYFKPEQYKTELKKIFQEIKLQEIWKKDSLNKILRKYPKDDKK
                     LFRHDELVYALKELGLQSKKIEERIRLKPTRTNSGVATVTVLTKPFPCPGRCIFCPND
                     PNMPKSYISSEPGAQRALTNHFDPYAQVYNRLIALKNIGHNIEKVELLVLGGSWSAYT
                     QDYQLWFVTQCYKAMNDVAEDSTEYISPKEDYESEVSFFNLEKEHLRNETAYCRNVGL
                     VLETRPDLITEEEVIHLRKLGATKIQIGIQSLNNRILKANNIGRKVKDVKKAIQLLRL
                     AGFKIHGHWMPSLYKATVDSDIADYKKLWRPSFSPDELKIYPTSIVPNTYLYTLYKKK
                     MYEPYTEDELLEVLVSVMPSTPRYCRLSRIIRDIPSEEIVAGNKKTNLRQIAEKEIEE
                     RGLKLQEIRSREIQDSSVTMKDLEMEIINYKSSIGKEVFISFKTKKTDKICGFLRLCL
                     PNFFYKRFNYIEELRDSSIIREVHVYGKVMSLTEDSSGQPQHLGLGKKLIQKAEEISR
                     ENRFKNISVISAIGTREYYKARGYDKVELYMKKRL"
     gene            8281..8820
                     /locus_tag="PKI16_01685"
     CDS             8281..8820
                     /locus_tag="PKI16_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNR53169.1"
                     /translation="MDQKQNKKEIIYAFIDSNNLYQGLLTDLPLRKYKGWRIDFEKFK
                     TYLEKKYHVEKLFLFMGYMKDNEKMYDDLKKLGYTLIFKPTISHTDTKGKTTVKGNVD
                     AELVLQTMIELPNYDKAIIVAGDGDYRCLIEYLDKIEKLKHILIPNQKLYSSLLIPFK
                     KYMIYMNPLRVVVGSKEKK"
     gene            complement(8818..8890)
                     /locus_tag="PKI16_01690"
     tRNA            complement(8818..8890)
                     /locus_tag="PKI16_01690"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8855..8857),aa:Lys,seq:ctt)
     gene            complement(9017..9391)
                     /locus_tag="PKI16_01695"
     CDS             complement(9017..9391)
                     /locus_tag="PKI16_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53170.1"
                     /translation="MNKILNIDNFEMVLFLASFVFFMLFLIRLIQIFKKPKNSTTWEA
                     TIVSKKKRNFITDYLETFFTFYILDDTFWIFGKKPQYIFKFKRDDGIVGKYFTGNKEF
                     ISLNVGDRIILKKGNFMPIKKV"
     gene            complement(9448..9520)
                     /locus_tag="PKI16_01700"
     tRNA            complement(9448..9520)
                     /locus_tag="PKI16_01700"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9485..9487),aa:Lys,seq:ctt)
     gene            complement(9570..11696)
                     /locus_tag="PKI16_01705"
     CDS             complement(9570..11696)
                     /locus_tag="PKI16_01705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNR53171.1"
                     /translation="MNIKTALKFLFTVFFLFVPFGFVKATDLPEQNFGVTVNAGINVD
                     LYSQLDINPIVVELKESSQVTLSIVDSLSNPRGNRNIQIYINGSSLGVTITQPPLTDL
                     LGYTVGSVKSSIPGSYEVCARDVTDSLHIDILDCEVLYVTPVAIPTIFTEPPYTIGTK
                     NTILWNTSGSGSYTYYAQVSTKADFSSVVNNSGWIGAMGYEFTNLQNGQIYFYRVKAK
                     NTSGGESAWSNTVFSVQDSEKPYITLLSVSGLGNNTVEDWNPNDTLIIRLRVKDNISV
                     ASKSFWCVLSDDSPDSCVDTETSVGDIWEVRIKLGDLEQDINGYLLEEYTFCAEAVDS
                     VGNVRRICNINLYVLHTQGPIPPKKTPIDKVVDKTKEIIDDTIGKIDVEDLQDITVTT
                     VATNVAIGVGILIGSFNSIPYFIIQLILSLLSILGFRKKGHPVGYVYDSVTKEPISQA
                     IVRVFNVNGILVWTDVTDGNGYFSGTHLNRGTYSIKVTAKDYTFPSKIVFGKEDFPLE
                     NVYHGREFSVRDEEIPNFSIPMDRVEYGAFRIFFEKIISQSKFVWKSLHVILFVVGLL
                     FSLYALYISRIWFNYLIVAIYIPALVLLVVSLFGRKGKYGVVRNTKKKRLQGVIVGLR
                     EKEFGKLVSKRVTDHLGRYRFVVNSGEYAIEILNSGIAITNYIGLDSIKVKDNTVIAK
                     DLVVKKIVEDGNEEELEEILEPLPEL"
     gene            complement(11696..12304)
                     /gene="recO"
                     /locus_tag="PKI16_01710"
     CDS             complement(11696..12304)
                     /gene="recO"
                     /locus_tag="PKI16_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNR53172.1"
                     /translation="MNRDTITVIKSINYSEADKILTVFGREFGKFSLFAKGIRKINSK
                     NRGNMQTLCTSDISFYQGKGMPVLTESVLIRSLDMDKLQVKNIERVLFILNKFLQEYD
                     PYPSIFDGLQSLLKREGDLEATNKLRIKILKDMGFLSDFSSCSLCGSKEDLKYLDTTS
                     FALVCKNCYSKGNGKPLGENPYSSKILTDALDRYVRRIVEEI"
     gene            complement(12297..12941)
                     /locus_tag="PKI16_01715"
     CDS             complement(12297..12941)
                     /locus_tag="PKI16_01715"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007475154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNR53173.1"
                     /translation="MLKSSIKRIFDILFSAVILIVLFPLLLICSLTIFLTDRKEIFVK
                     EPLRLGRKGEFKMYKFRSMIPNAHSEIESNPKYKELKKKWQEKDGKLKLSEDPRITWI
                     GKIIRKTDIDELPQFLNVLKGDMSVVGPRPMYQAEVNRYLKAYPDGKKYIKQILKIRP
                     GITGIWQVSGRNGISFEKRIQLDAKYSKDINLLGDLKILLKTPYVVLTRKGAYE"
     gene            complement(12928..14103)
                     /locus_tag="PKI16_01720"
     CDS             complement(12928..14103)
                     /locus_tag="PKI16_01720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR53174.1"
                     /translation="MSPRKKKRNIPEETLKVAVVAEPLFKHGGAEVHLRYIIKAFPNC
                     EVYTAYYDKKFVKEFFGDIKIHHSFMQYLPKKFEFRQLYLLFQPLAYRSFKLKGYEVI
                     VSLSIAFAKFAHAKGVKHIDLCMTPPKFFWEKEGRSIKNANQFKGLNKFLFKFYSFFM
                     DTFLEKLWQKWDKQAAQKCDKIAAISKIVKKRIKKYYELEADVLYPPVEVKRIQSYKK
                     MNRKENWFLYMGRVETYKGVDLAIKACAMADVPLKIGGTGDHLEQMQELVKDMNAKGL
                     IKFLGYISDDEKINLLSRCRALIFPVRNEDFGMIPVEANAAGTPVIAYREGGVLETIS
                     EDNPKTGVFFDKYDVETLAETLKNFKDSSYDPDNCRKQADNFAAEIFMYKLRNYVKE"
     gene            complement(14057..15214)
                     /locus_tag="PKI16_01725"
     CDS             complement(14057..15214)
                     /locus_tag="PKI16_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR53175.1"
                     /translation="MYKLEKLFPRVAIVADQMTSYGGADAELLSMLKILPHADIFTIL
                     FDKKKYPGLTNRVYTSFVQNIAKFLPKGFSRHLKIFTPLAYESFNFDGYDLVISISAG
                     PAKGIITEIYQPHVAMVMTPPRSLWDNELNVRGSKLRWLYRPLSRVLNTHLRIWDWSI
                     SRRVDYWTANSKYVAQKIKKTYGKDSTVIYPGIVKENFKKIDVRKKYSLPKKFVLVVS
                     RLYDYKRIDWAINACKETNTNLVIVGEGPDLRYLKKIAKRDKRITFLGFVPDAERNAF
                     FKEAEVLLFCGVEDFGLVPVEAMSYGTPVLAYKEGGVLETVIEGVTGEFFKDEKELSN
                     LLKNFNKRRYNEQNIISQAEKFTETKFLNNLEQFLINVSKEEKKKYSRRNP"
     gene            complement(15221..15721)
                     /locus_tag="PKI16_01730"
     CDS             complement(15221..15721)
                     /locus_tag="PKI16_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53176.1"
                     /translation="MANPAKKKKDELIPQRKSVHVVFFSCAECGEEVDEIKICPECGK
                     PMKVIDVVEKFGEEADRYLEKAILPKKNGSEEEEYVSIDKEEPNIILMGGDEDIIGDG
                     GIDPSQDDGESLDVIFPDDDHDDSPKAEVPIDEELSKALEQLDSEEEDVSAEDFGFDG
                     EDIPEL"
     gene            complement(15801..18719)
                     /gene="secA"
                     /locus_tag="PKI16_01735"
     CDS             complement(15801..18719)
                     /gene="secA"
                     /locus_tag="PKI16_01735"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNR53177.1"
                     /translation="MIKFLEGLFDSNEKQLKKIQPLVEKINGYEDEISKLSDEELRAK
                     TEEFRKKIFVDLETCRTDFVNKKESVLVEELEREKRRLYEILPEAFAVVREASKRVAN
                     HRHFDVQLTAGYVLFDNKIAELFTGEGKTLAANLPLYLYGLTGRGAHLVTVNDYLARR
                     DAEWTGHILNALGMSVGAINSGKQYRFITDEEALKIKGDDAKKLIKERDEKVKASGRL
                     KYDHMSGVNLLECSKQEAYGCDVVYGTNNEFGFDYLRDNMARTKEERVQGPKYFVIVD
                     EADSILIDEARTPLIISTSAQGANDLYKRFASLANSLQKDKHYAVDEKAHAVSLTDEG
                     IDAVEKALGVKNIYEDAQLTYHLDNALKAKELYQLDDEYMIRNGEILIVDEFTGRAME
                     GRRYSEGLHQAIEAKEGVEVKRESRTMATITLQNFFRLYKYLSGMTGTALTEAEEFSS
                     IYKLDTIVVPPNKPIIRKDYNDVVYRSQEGKFKAIVEDIKEHHAKGQPILVGTTSVEK
                     SEILSSMLSKEGIKHNVLNAKQHEREAIIIAEAGQKGSVTIATNMAGRGTDIALGEGV
                     KEVGGLYVIGSERHESRRIDNQLRGRSGRQGDPGESRFFVSFEDELMRLFGGDRMQLM
                     MTQVGMDDDMPISLGILGKTIENAQKKVEARNYDIRKSLVEYDDVLNQQREIIYTLRR
                     KILDIELKTDFNVTDKTLKKDLDIVLNADIEGIQDALDALALNREETWKIEELDNLNF
                     ILSPLEKWILKKEFEYIDFLQASQLVDDMKISNVEERQVYQQVMNLLTDEIFEEAVKK
                     MGFKTSVEMFKDLEKKTNVEQAVQLKKLILVSFILHIYAIGVEPMKALSRLLLLEAFD
                     RFWMEHLDTMTDLREGISLRNLAQRDPLVEYKNEGFRMFDDMLKKVDESIVTRFFKVR
                     LVKREEQIPVRVNTDEDKRPGNVGKQKTIKKNKKVGRNDPCPCGSGKKYKKCCFPKYG
                     "
     gene            18778..20292
                     /locus_tag="PKI16_01740"
     CDS             18778..20292
                     /locus_tag="PKI16_01740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNR53178.1"
                     /translation="MYKILFSFLFFFLSTTACSAELLLISPQIDEYLTNRVVTFKWKN
                     TDTQNEYVYRLDIAFYLNWSTYMLIPVESNTTYTQTLSNYDVFAWRVLYARKNTFDGT
                     YPHVSETRRFSINTDLPQEEIPEPRPEPEPESKPKEEEKPKPIEKPKEKIKPTKKVVE
                     MPKNEEEHMEWDISTASVLGANKKEVVCRFKYLKGKSEIVSCKIPSLQLQEITQYPFV
                     DEYSVVVKGKFLDDISIVVDEYRCKRDILRPISLFKCEEQLVSTHDITVEPNIFFNIY
                     QGDTFVPIKSYTRDGEQFTLVGGYFRKEENLRMRFRYSVNISQFNISISGGGSYSLNP
                     KNISNSKKPFSFPFSKVIGVTQWHGNTAFQRPHKGIDFGATRESVLAVYDGSVVGKGW
                     DSYNGKCFSGGNYLLIKQDNGMYTTYFHLEKTSVNTGDRVKKGQIVGVSGNSGAWNCQ
                     KLGYHLHFEIRKSRSQSTHVNPVDYINTDWSKVVTLNAKYIPGRLSGENPHPGY"
     gene            20308..20940
                     /locus_tag="PKI16_01745"
     CDS             20308..20940
                     /locus_tag="PKI16_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53179.1"
                     /translation="MEYLKMLVFLFFSLFSQEKVEYMLPNIVDVQQIVEVTPQKVLLV
                     NEGDILQLDVLGRKVKKVGKRNGNEFVGYDGGLLFCKIEHYIIDSEDEFSTKFTVLDE
                     EKDVVRELQFFETIRPLYIDNNIIVATTAIDFLGRHFYKISVESGEMKEIFLEDLGQK
                     KNITNVREDVFGNVWVSFSTKKVVLTHILTHLSGLILQLEQLGIYQKLSL"
     gene            complement(20861..22093)
                     /locus_tag="PKI16_01750"
     CDS             complement(20861..22093)
                     /locus_tag="PKI16_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNR53180.1"
                     /translation="MKFFRILKQSFKAIMANKVRSFLTVLGIIIGIGSVIGLMSLGTG
                     VKESINGQINSLGSTNLTIMTGFNMSSRDSYTTNPQEAPKANGAMFGSSQSLTQEDFL
                     ALEKISSDLVSNIAAYASSQVILKTNGKEQRGTIIGVSSDYFTFYDLNLSSGEYPKGK
                     EVVLGDQLAKDLFGDTNPIGQDIPIMDIKFKVSGILEIQDETNFSNPNLQAYITDQEA
                     FKLFGTDNYNSIIAKATSEDTVDDAKTEIESVLLTTHKIDDKNLADFFITSPNDLLST
                     VDTVMTMLTSFLAGIAAISLLVGGIGIMNIMLVSVTERTREIGLRKALGAQTSNILIQ
                     FMTEAVVLTVIGGIFGILTGYVISLIASKFLGFDAIITFDSILLAVSISSFIGIIFGI
                     YPAARAARLNPIDALRYE"
     gene            complement(22090..22773)
                     /locus_tag="PKI16_01755"
     CDS             complement(22090..22773)
                     /locus_tag="PKI16_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNR53181.1"
                     /translation="MLENIIELKNISKIYKSEGGNTLALDDIDLNIQEGEFLAIMGPS
                     GSGKSTLMNILGLLDTPTSGTYILNEQKVSKLKERELAKIRNKEIGFVFQQFNLLART
                     TVFDNVLMPTIYGNIPNPKEKAEALIDMVGLKNWSKHKSNQLSGGQIQRVAIARALMM
                     DPSIILADEPTGNLDTKRSEEIMELFKEINKKGSTMVLITHEEYIAKYASRIIHIQDG
                     KVVKEEYNR"
     gene            complement(22794..23279)
                     /locus_tag="PKI16_01760"
     CDS             complement(22794..23279)
                     /locus_tag="PKI16_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNR53182.1"
                     /translation="MLEIIIYILLALVSLAGLVLVAINLPGIWLIYISTVVLAIMDKF
                     ENLTPLVLVILFLLCLLSTLSDNIATALGAKKMGGSGWGIAGAIIGGLIGMLAGGIAG
                     MFLGPLFGATIFEIIFAHKNATQGVKAGFGTFIGIIFSIIFKFSLSVGIIIFTVSRIV
                     N"
     gene            complement(23311..23478)
                     /locus_tag="PKI16_01765"
     CDS             complement(23311..23478)
                     /locus_tag="PKI16_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53183.1"
                     /translation="MIIFIDESGDLGILERIFGKKRPSPYSRASLETHTYKASVQKPS
                     LFICIISTYGI"
     gene            complement(23527..24702)
                     /locus_tag="PKI16_01770"
     CDS             complement(23527..24702)
                     /locus_tag="PKI16_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNR53184.1"
                     /translation="MHIIIDATTTQDQMAYAGPGRYTRNIILSLVKEYPDTKYSILLF
                     EDKESTLDTEISKYPNAQIIRIGKYKDNDYKNDITYLTQILPAIKKIKQKDSIYFCPY
                     FWRNFPSYTMPVVLFIHDMNLPMFNMYSQQSPIHNFIRKIQYWMTLNKAMKCKHIITN
                     SQNTKTDFLKYYPKYPSEKISVSYIGVEIEEKETSLDNILPKDWKEKKYLIYLGAGIN
                     RTKNSKGALLSYVSFLKTLDKEQAPYFVIAGGKFEDKTKPEIKELYDIIEKNNIQDKV
                     IFTGFYKDEDKYSLLHNSFAFIHLSLAEGFGIAPIEAMRSKTPTILHRSPVYLELFSD
                     VSVMVDGNNPGLVGCVIKDIYMNEDKYKELVEKAYTLSKEYTWEKSAEITHGVFEKI"
     gene            complement(24703..25554)
                     /locus_tag="PKI16_01775"
     CDS             complement(24703..25554)
                     /locus_tag="PKI16_01775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNR53185.1"
                     /translation="MKVFVLIPTRNRKDLLIKCLDSLLKQSYSDIEIVVIDDGSTDGT
                     GDILKKKKVTVLEGDGNLWWSGAMRMGVEYVLPKSKATDFVLIQNDDTYMEEDFLEKL
                     VKQSQENNRIILGTPVIDSVSRKLIHDSHKIIHGSFRPVIIDSKEEIISADTLSGRGA
                     LIPIEVFHKIGNFSKIFPQYASDYDFFCRAKNHGFKLGVTTKTETISTNSRPNLSRRV
                     KAQSKLTFRDFFQLYFSRRSSNNLWSSTLITLMYSPWKYKLYGVIRIYGVFIKTFFVN
                     VLIYSFK"
     gene            complement(25564..26289)
                     /locus_tag="PKI16_01780"
     CDS             complement(25564..26289)
                     /locus_tag="PKI16_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR53186.1"
                     /translation="MLLNQITNKFTKIIEFPFQVLRMQIKQNECQSLKDNIKLAKLRN
                     RAPKCFDRDFEYPWVLKNIDIKKGKLLDIGSTVGQMLYDILPKTVEINTLNINSQKDV
                     KGVKQIEGDIRKTDFKSNLFDCITCVSTLEHIGVEGRYGVEGDETGDMKAMKEMYRIL
                     KPGGKLFLTVPYGAQDVLPINRLYNKNRTEKLFKGYKLLKEEYLKYNKKYGIWLTVSE
                     KEAAETIWQKDKWYSLGLFILQK"
     gene            complement(26302..>26814)
                     /locus_tag="PKI16_01785"
     CDS             complement(26302..>26814)
                     /locus_tag="PKI16_01785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR53187.1"
                     /translation="IDEKDLPLWVDVAEEVKKIRGTVSGVMLGDGVRMGMIEKMIEEK
                     GLKEDIKLVGRTSDIYPYIDKSKILLMTSKSEGLPMVAVEAMSIQVPCVLPDVGDISD
                     LVTDGINGRLINTRNPKDYADAVISLLKDETEYREISKEAYESIKKMAQESTHDKIVK
                     LWNSKLKDIN"
BASE COUNT         8333 a         5123 c         4203 g         9155 t
ORIGIN      
        1 ttaataatta gccagatgat gcaatcttct attgatattc ttccatcata atactatatc
       61 cagttacatc tagattttga aatctaatca tcattccggc aatatttcca tttggataca
      121 caagtcgtct gtgataaata tagttaccat atccatcatc aaaagtgttt acaacatctg
      181 gctcaccaaa aatttccttt acctctgaaa tattttcaat tttaaagtca ctaaaaagtt
      241 cttcaaactc ttcgaccggc atcagtttaa tttcatttct aggactttcg ctaatttcat
      301 cttttttccc ttcggtttgc tcaacattat tttctttttc gtttttagca acttctttct
      361 tagcgactgc ttcggttggt tcagtctctt tctttgtgat ccctaggaat tcttgtactt
      421 cttcctctgt cattggttta taatctttgt taatactctt tatccactcc tctacctcag
      481 ccatttccat ttctgcaaca gttacatctc caggatattg agtgttttta attacttgaa
      541 atggtggatg atattcaaaa tttttaagat ctattccaat aacttttcct tcctgaactg
      601 tatccctaaa ctgttctaat ccaaccataa ctgaatcaaa cttaccataa gctgaaccca
      661 gattactggg aatattagtt atagttaaaa cagatgctat tggttctgta actgttctaa
      721 catccttgac aaaggaagat accttattcc tatcaccaag tgctatctga gcactatctt
      781 cagttacctc caatgctaaa tccacaccta caacaactgt agtcacttga gatacaattc
      841 ctgcaccacc accagttgct acaatacccc ctacatagac agtaacttta catgtatctt
      901 taaccactat tgcctgattt tctaacttct caaaagtatc tcccgcttct ccccacgcct
      961 ctctagttgc catatcttga gattgattta gtattaactg tgccctttta gcatctactc
     1021 ctagatgttg agccaatgtc cttatctgtc ttcctgcagg agctttctct actattgctt
     1081 gtacttcagc atagctgtag tcctttgcat atgcactctt tattatgtta aatgctgttg
     1141 cctcattaga catctcctct gcattctcct ctagttttaa tgctttactc tctaatgttt
     1201 cccactcttg aataattgag gcaatctctt cattccaatc atcgtagctt tcataatctt
     1261 ttgcatttac taaaacatta tctgttttgt atcttaatac tagatactct ttagatattg
     1321 ttaatgtatc ttcatatact atctgatctg tctttttatt atttccaaaa agaaggaaag
     1381 ccagaactga aaatatgata atagaaaaga caaggagaaa aatattcttt ttagacatgt
     1441 tctttcttaa aaaagattta gataataatt attatagtac aaagaatata ctactccaat
     1501 gggttattct ggttctaaac gtgtatattc cactaaactc tacatatttc aacaagagcc
     1561 aagatttaag attccaaaag aatctttatc ttcttcttac tatagatctc taagattata
     1621 ttatctttca attacaccta tatcccatat tacaattcta tttatattag ttaaattgat
     1681 tttttcagat atacttccgc cttctttcca cagataataa aaagatgcta gtatttacga
     1741 tgaaatataa aatatgccag atgcaagaga atgatatatc tgaagttata gatttccata
     1801 ataaatatct tgggacaaga gattttatta acaaaaaaga aatatctaac agattaaaat
     1861 ctaataatgg aattttcttt gttgcgaaag atgaaaaagg gaaaataata ggcataaagt
     1921 taggatacat agagaatgat acatgcatag gaagaggtat agcagtagat aagagataca
     1981 gaagacttgg aataggaaag gaattggtta agaacttcga agatagatta aaaagctttc
     2041 cttcagtcaa gaaatatata tttgcatcgt ctacacaaga aggaattcca ttccatatca
     2101 agttaggata caaaccatca gtattattac agtcaaataa taaagagctg ttagaatcca
     2161 taaacttaga agagtttaca ctcaaaggga agtcatacaa caaggtttac aatgtacacc
     2221 agatatactt agaacctgac caagagccag atcttagcta tctctctcag attaaatcta
     2281 gatgctctgg aatagatatt cagtatctgt ttgagaagga tttttaaagt aaatacataa
     2341 tctcattatg tagaagaatc catccaaaag aaaacgctat tctttttctt aaacttgtaa
     2401 caatactact agagttatgc ctctaataat ttgatatcga ttgtattgag acccttttgt
     2461 gtttcttcaa tatagaaaaa cttaattgtt tttttccttc tctatatatg taacaactac
     2521 atcaaaatct attgtggaag ataattagga aaatatttga cttcgcagca ttactgtcct
     2581 acaattaaat tccaagtagt atatacataa tatttacctt aaatatatga aaattacttg
     2641 gaccccttcc tcttctagtt ctactcaaac accatacacc acttcaccta ctccctacaa
     2701 aacataccaa acttttacac ttggaaaaga cactcccata acagtattac aatcccttca
     2761 tacaagatct ttactaatta caaaagacaa caaaccagca cgagtgtatc ttggtatggc
     2821 aagagctaaa gatctctgta ttgccacata taccatagcc tctacaaaag aattcaaaaa
     2881 cgcaattaac cacaaatata tacccgaaaa gaacgccatt agtgttatgc taattcgtga
     2941 accattagct caatcaccat tcataaacac atatatgggt agtggatttg aatctaggct
     3001 gtatgcagtt atggatgtac atcatgtaaa agatgttaaa ggattaaagg gtatatctgg
     3061 aagagtatgt agttacaaaa tagatatacc agagagtaaa tcaagagaga tacatgaaga
     3121 tattcggttg gccataatat atgactcaat tgcaggagga agaaatatca tagcttcaat
     3181 aagagaacta aaaaaacaat ttaagaattt aaaaaaggta gtactcttct ctgtatactc
     3241 tacataccaa ggttcaaaaa gaattgccaa catatgtaaa aagaataagc ttgcttgtga
     3301 gttcttctgt atgcatgaat tactgaatgc aagtccatta aatgagtatg actgcttcta
     3361 tccttcttgg aatatatcta aagaggatga agatatttta aaatctttct acggcgagaa
     3421 atataacaaa atatgtatag gaggagattg gactgcgaat acacttggta aagaacaagc
     3481 tgttgatgta tttaaatcac aagcaaaaga actgggtata gatacaagta gatattttta
     3541 gtatattatc tacatatgtc caagaaaaaa gagaaatcat ttctaaacat ctttcttttt
     3601 gtattccttt ttctctttgg gctagaactc accttcgcaa tcggatatct tcttaaatca
     3661 cctctacaaa aaacaatctc ctctattcca ttcacatcat ttacatttga cacctctaaa
     3721 ctaacatacc aacaaccaga acatacagaa ataactccgc tttcatctct aactatactc
     3781 ccaaaagaat acacatcttt taccaaacag tacggcaccc ttacacagca tgaaaaaaca
     3841 gaactcctaa gatactctac aactacacat agaacagaaa gcaaaactct tacagaactc
     3901 tatgcagata tatacaacaa caatctaaat atcctcatcc cttcccagta tctagacata
     3961 tatttcctga ctcaattcca agacattgca aacagaataa aagaggaaga tacctttcct
     4021 tcaacctccc aagagctatc ccgcatattc caaaacggaa ttacccttta caatgattca
     4081 gaagtagaag atattaccaa tgtctctgac ttttcaattc tctctaagtg gttacttaca
     4141 caaaaagaca tcacagagga aaatacttac actgccttct tagacaatat ctacaacaaa
     4201 tatattacat ctgagaaatc aacagaaaca ctagcaacag aaatcttaaa cgaagaatat
     4261 ataggtttga tatacttcga caccaatccc ttacaacttt ctaaaatcga ctacatacta
     4321 caatttttag ctgaggtata cacacaggca aaattagaca acaaagaaat tctctaccaa
     4381 attaacactc taaaacagta ttctcaagat ttgaaagatt caaacactgc agatatcccc
     4441 ttggaagaaa ttttaacaaa tccaaatctt aatatatcgg ctccagagga accagtttct
     4501 cttttaatta aaacattcga agacaaaaca tatctcgctc caacatacac attctccaaa
     4561 gattttgttg taaatgacaa aaatttcgat gagccatatg atgtaaaacc aattgctcaa
     4621 tccaaaggat caactagagt tccaatcttc atgtaccacc aaattggtaa tcctccaaaa
     4681 ggagcttcca agttcatcgc tggtctctac attactccgc aacaattcga agaacagatg
     4741 gcatatctag taaaaaacaa ttacaaaaca attggctctc tagagctgta taacctgctt
     4801 aaaaaaggtg gaaacccatc tcagaaaact gtcatgctta cattcgacga tgggacatat
     4861 ggacagtatc agtatgcatt cccgatacta aagaaatatg gcttaacagg aaccttctac
     4921 gtcatttcaa ataggtctag catcggtaag acatccatct tgaaggagat ggcacaagca
     4981 ggtatggaga tagcatctca ctctgcaact catcctaatt ttgttaacct aaaagacctg
     5041 gaccagttaa catacgagat atattcaagc aaaagtatgc tagcagcaag gacaggaaaa
     5101 ccagtatatg ggatagcata ccccggatgt gtggcagata gtcgcgtctt ttctagagta
     5161 gcaggtgctg gatatttaat cgggggaagt tgtggaaaat ccatagacca ctatctaagt
     5221 aaaagacttt cactctctcg tgtacatgca tatggagata tgaagagttt taaaaagtta
     5281 ctgtcaggag catactaatc aggtataatg gatatatgaa aaatgatttc tcttctttaa
     5341 aagaattaca tgaatattat gcagtaaata acaactgcaa attaaaaaac attgctaccc
     5401 aacctgtgta tgaaataact ccaccatcat ccggaatagt gttcatagga gaagcccctg
     5461 gtaagaacga agatttacaa ggaaaaccat ttgtaggagc agctggcaaa ttcttagatc
     5521 aattacttaa aggtattgga tttaccagaa acgatgtata tgtctcaaat actgtaaagt
     5581 acagaccacc agaaaataga gacccaacgg atttagaaaa agatagctgt agagtatggc
     5641 tcaatgcaga gcttctcttt atacaaccaa aagtaatcgt tcctcttgga aaacatgcat
     5701 tagcaagatt tgtcccagat acagcaataa ctgtagtaca tggaaatcca tattcccatc
     5761 ccagtggtat ccccatattc cctatgtacc accctgccgc tgcactctac aacccaaact
     5821 taagagaggt attacaaaaa gattttgaaa agctcaaaga attcctaaac aaaggaggaa
     5881 agttactaga aaacagtatc gaagattcct ctggattctc caaacaatca aagcaggata
     5941 tagatgaact cttaaaactg tagtttagac attgtcccaa cctgcatcat agaagatttt
     6001 ctctgcaaca gtttcgtagt tattacaaat ctccttttct ttaaaccata tcttaatttc
     6061 tctttttgca ttctctggag aatcagaagc atgaagcata gttctcgtag atcttgcaac
     6121 aacatcagcg agagcatacg aatcgaaaga gtaatctgca cgtattgtac caacatcacc
     6181 atctgcagga cttgtactac caagaacctt tctaacacca gctacaaccc ctggtccctc
     6241 cagaactaca gccaatattg gtccacatgt aagatatgca atatttctct ccctaataat
     6301 cttcccccat tctaatgaat tccaattcga aacatcctgt cctctcatct gtgcaccctt
     6361 ctttgctttc tctcctgtct caattaagta ctgctcatca ccagtatagt gttctccaac
     6421 ctgctcctta gttgcattaa ccatcttcat agcaacaagt ttaaaacctt ttctctcaaa
     6481 tctagaaagt atttctccca caatctgtct ttgtaataca tctggtttca tcagtattaa
     6541 tgttctctct tccgctatac ttggtctgtt catatagata tggtgttaaa aatattagat
     6601 aatacaagga tatcaaaata atggaatact tcaaaccaga acaatataaa acagaattaa
     6661 agaaaatatt tcaagaaatt aaacttcaag aaatctggaa gaaagattct cttaacaaaa
     6721 tactccgtaa atatcccaaa gatgataaaa aactcttccg tcatgatgaa ttggtatatg
     6781 ctctcaaaga attaggatta caaagtaaaa agatagaaga gcgaattaga ttaaaaccaa
     6841 caagaacaaa ctctggtgtt gctactgtta cagttctaac caaaccattc ccatgcccag
     6901 gaagatgtat cttctgtcca aatgacccaa atatgcccaa aagctacata tcttccgagc
     6961 caggtgcaca gagagcactt acaaaccatt tcgaccccta tgctcaagtt tacaacagat
     7021 taattgcact taagaatatt ggacacaata tcgagaaagt agaacttctt gttctaggag
     7081 gaagctggag tgcatatact caggattacc aactttggtt tgttacacag tgctacaagg
     7141 ccatgaatga tgtagcggag gacagtactg aatacatatc tccaaaagaa gattacgaat
     7201 ctgaagtttc cttctttaat cttgaaaaag agcatcttcg caatgaaaca gcgtactgta
     7261 gaaatgtagg cttagtcctt gaaacaagac cagatttaat cacagaggaa gaagtcatac
     7321 atcttagaaa acttggagca acgaagattc aaattgggat tcaatctcta aacaacagaa
     7381 tacttaaagc aaacaacatt ggaagaaaag ttaaagatgt taaaaaagcc atacaacttt
     7441 taaggcttgc tggatttaaa attcatggac attggatgcc aagtctttac aaggcaactg
     7501 tagattcaga tattgcagac tacaaaaaac tttggagacc atctttctct ccagatgaat
     7561 taaagatata tcctacctcc attgtcccca atacatatct gtacactctc tacaaaaaga
     7621 aaatgtacga accatataca gaagacgaac tactggaagt tctggttagt gttatgccct
     7681 ctactccaag gtactgcaga ctctcaagaa taatacgaga tataccctct gaggagatag
     7741 tggcaggaaa caagaaaaca aatttaagac agatagccga aaaagagatt gaagagagag
     7801 ggcttaagtt acaagagatc cgcagtagag agatacagga ttcgagtgta acgatgaaag
     7861 atctagaaat ggagattatt aattacaaat cttcaattgg gaaagaggtt ttcatatctt
     7921 tcaaaaccaa aaaaacagac aagatatgtg gctttcttag actatgcttg cccaatttct
     7981 tttacaaaag atttaactac attgaagagc ttcgagactc ttccataatc cgtgaagtac
     8041 atgtgtatgg taaggttatg agtttgactg aagatagcag tggtcagcca cagcatctag
     8101 gattaggaaa gaaattaata cagaaggcag aagagatatc tagagagaat agattcaaga
     8161 atatatctgt catctctgca attggtacaa gagagtatta caaggcaaga ggatatgaca
     8221 aagtagaatt gtatatgaaa aagaggttgt aactaccttc taaaaaggtt aacattcatt
     8281 atggaccaaa aacaaaacaa aaaagaaatt atctatgcgt tcatagatag taataatctg
     8341 tatcagggac ttcttactga tcttcctttg cgtaagtaca agggttggag gatagatttt
     8401 gaaaaattca aaacatattt agaaaaaaaa tatcatgttg aaaagctctt tctttttatg
     8461 ggatatatga aggataatga gaagatgtat gatgatttaa agaaattagg atatacctta
     8521 atcttcaagc ctacaatttc acatacagat acaaagggta aaacaactgt aaagggtaat
     8581 gttgatgctg agcttgtatt acagactatg attgagcttc ctaattatga taaggcaata
     8641 attgttgcag gtgatggaga ttataggtgt ttgatagagt atttagataa gatagagaaa
     8701 cttaaacata tattaatacc aaaccaaaag ctttactcta gcctactgat acctttcaag
     8761 aagtacatga tatatatgaa tccactgaga gtggtagtag gtagtaaaga aaagaaatga
     8821 gcgtacaggg gctcgaaccc tgaacctagc ggttaagagc cgcttgctct accaattgag
     8881 ctatacgccc taaaaaggac tgaagaataa ttctcttagt atgagcattt gtattttgtg
     8941 taggtcattc ataacagaat atatcaacaa atatctacat aatctagtga aattgatttc
     9001 attagaaagt attaatctac actttcttta ttggcataaa attccccttt ttaagaatta
     9061 ttctgtcccc tacattcaga gagataaact ctttattacc tgtaaaatat ttgccaacaa
     9121 ttccatcatc acgtttaaac ttgaaaatat attgaggttt tttcccaaaa atccaaaaag
     9181 tatcatctaa aatataaaaa gtaaaaaacg tttcaagata atccgtaata aaatttcttt
     9241 ttttcttgct aacaattgtc gcctcccatg tcgttgaatt tttgggtttc ttaaatattt
     9301 gtattaatct aattaaaaac aacataaaaa aaacaaaaga ggctaagaac aaaaccatct
     9361 caaaattgtc gatatttaat attttattca tctttagaat aatatagaga attatcccat
     9421 tattaaatat ttgatacaag aagaaaatga gcgtacaggg gctcgaaccc tgaacctagc
     9481 ggttaagagc cgcttgctct accaattgag ctatacgccc taaaaaggac tgaagaataa
     9541 taccattata tctaccattt ctcaatatat tacagctcag gaagtggttc cagtatctct
     9601 tctagttcct cttcgtttcc atcttcaaca atcttcttta caacaagatc tttagctata
     9661 acagtattat ccttaacctt aatactatcc aaaccaatat aattagttat ggcaatacca
     9721 ctattcaata tctctatggc atactctcca ctattcacta caaatctgta tctaccaaga
     9781 tgatccgtta ctctcttgga taccagttta ccaaactctt tctctctaag gccaacaata
     9841 actccctgca atctcttctt ctttgtattt cttaccactc cgtatttacc ctttctccca
     9901 aacaaagaga ccaccaacag aacgagagca ggaatatata tagcaactat taaatagtta
     9961 aaccatattc ttgagatata caaagcataa agggaaaaca gcaaaccaac aacaaagaga
    10021 ataacatgca aactcttcca aacaaattta ctctgtgaaa tgattttctc aaaaaatatt
    10081 ctaaatgcac catactctac cctatccatt ggtattgaaa aatttggtat ttcctcatca
    10141 cgaacagaaa attctctccc atgatataca ttctccaatg gaaaatcttc tttcccaaaa
    10201 actatcttag atgggaatgt gtaatccttt gcagttacct ttatggaata tgtacccctg
    10261 tttaaatgtg ttccactaaa gtatccatta ccatctgtta catctgtcca taccaaaatc
    10321 ccatttacat taaatactct tacaattgct tgagatattg gttcctttgt aacagaatca
    10381 tacacataac caacaggatg acccttcttt ctaaatccta agatactcaa aagacttagg
    10441 atcaattgga ttatgaagta tggtatggaa ttgaaacttc caattaaaat accaacccct
    10501 atggcaacat tagtagctac agttgtaaca gtaatatctt gtaaatcctc tacatctatc
    10561 tttccaattg tgtcatctat tatctccttt gttttatcta ctaccttatc tattggagtc
    10621 ttcttaggag gaattggacc ctgcgtatgt agtacataga gattaatatt acaaattctt
    10681 cttacattac caaccgaatc tacagcctct gcacagaagg tgtattcctc taggagatat
    10741 ccattaatat cttgctccaa atctccgagt ttaattctta cttcccagat atctccaaca
    10801 ctggtttctg tatctacaca actatctgga gagtcatcgc ttagaacaca ccagaaactc
    10861 ttgcttgcaa cagatatatt atcttttact cttagacgaa taattaaagt atcattggga
    10921 ttccaatcct ctacagtatt gtttcctagg ccactaacac tcaagagggt tatatatggt
    10981 ttctctgaat cttgtacaga gaagacagta tttgaccaag cactctctcc tccagatgta
    11041 ttttttgctt ttacacggta aaaatatatc tgtccatttt ggagattagt aaattcatac
    11101 cccatggcac caatccagcc actattattt acaacagaag aaaaatctgc cttcgtacta
    11161 acctgagcat agtatgtgta agatccactc ccagaggtat tccacagaat cgtgttttta
    11221 gtgccaattg tgtatggtgg ttctgtaaag attgttggaa tagcaacagg agttacatac
    11281 aacacctcac aatccagaat atcaatatgt agtgaatcag ttacatctct cgcacacact
    11341 tcgtaagaac ctggtattga agatttaaca gaaccaactg tatatccaag caaatctgta
    11401 agaggtggtt gggtaattgt aactccaagg gaggaaccat ttatatatat ctgtatattt
    11461 ctattacccc ttggattgct aagactatct acaatactca gggttacctg agaactttct
    11521 tttagctcta caactattgg attaatatct aactgtgaat acaaatctac attgatacca
    11581 gcatttacag ttacaccaaa attctgttcc ggaagatctg ttgctttcac aaaaccaaaa
    11641 ggtacaaaaa gaaaaaagac agtaaacaag aattttaagg cagtttttat attcattata
    11701 tttcttctac tattctcctc acataccgat ctagtgcatc agtaagtatt ttagatgaat
    11761 acggattctc acccaaaggt tttccgttac ctttactgta acagtttttg cataccagag
    11821 caaaacttgt agtatctaaa tatttaagat cttctttact tccacagagg gaacaagagg
    11881 agaaatctga aaggaaaccc atgtccttta gtatcttgat tcttagtttg tttgtagctt
    11941 ccagatctcc ttctcttttg agaagagatt gtaatccatc aaagatactt gggtatgggt
    12001 cgtactcctg caaaaattta tttagtatga aaagtactct ctcgatattt ttaacctgaa
    12061 gtttatccat atctaaactc cttatgagga cagactctgt tagtactggc attccctttc
    12121 cctggtagaa ggagatatca ctagtacaaa gggtctgcat atttccccta tttttactat
    12181 ttattttcct tatacctttt gcaaagagag aaaattttcc aaattccctt ccaaatacag
    12241 ttagtatctt gtctgcttcc gagtaattta ttgattttat gactgtaatt gtatctctat
    12301 tcatatgccc ccttccttgt taaaactaca tacggtgttt ttaaaagtat ttttaaatct
    12361 cccaaaagat taatatcttt agaatatttt gcatccaatt gaatcctttt ttcaaaagat
    12421 ataccatttc tcccagagac ctgccatata cctgtaatcc caggacgaat ctttaatatc
    12481 tgcttgatat atttctttcc atctggatat gccttcagat atctatttac ttctgcttgg
    12541 tacataggtc ttggtccaac aacagacata tctcctttca gaacattaag aaattgtggt
    12601 agttcatcta tatctgtttt cctaatgatt ttcccaatcc atgtaatcct tggatcctcc
    12661 gacaatttta atttaccatc tttttcttgc catttctttt ttaattcttt gtattttgga
    12721 ttactttcaa tttcactatg tgcgttagga atcatacttc taaatttgta cattttaaat
    12781 tctccttttc ttccaagacg aagaggttct ttaacaaata tctcttttct gtcggttagg
    12841 aatatagtaa gtgaacaaat gagtagtaat gggaacaaaa cgattaatat aacagccgaa
    12901 aacagtatat cgaagattct ttttatacta ctctttaaca taatttctta gtttgtacat
    12961 aaagatctct gctgcaaaat tatccgcctg ttttctacag ttatctggat catatgatga
    13021 atctttgaaa ttctttaagg tttcagcaag agtttcgaca tcatatttgt caaagaatac
    13081 tcctgttttt ggattgtctt cggaaattgt ttccaaaact ccaccctctc tatatgcaat
    13141 tactggtgtg cctgcagcat ttgcctctac aggaatcatc ccaaaatctt cattcctaac
    13201 aggaaaaatt aaggctctac atctagacag taaattaatc ttttcatcat cagagatata
    13261 tcccaagaat ttaattaacc cctttgcatt catatctttt actaattcct gcatctgttc
    13321 aagatggtct cctgttccac caatcttgag aggtacgtct gccatagcac atgctttgat
    13381 tgccaaatct actcctttgt aggtttcaac tctacccata tacagaaacc aattctcttt
    13441 tctgttcatc ttcttgtatg actgtatcct ctttacttct actggaggat aaaggacgtc
    13501 tgcttctaat tcataatatt tctttatcct tttctttact atttttgaaa ttgctgctat
    13561 cttgtcacat ttctgtgccg cttgtttatc ccatttctgc cagagctttt ctaggaatgt
    13621 atccatgaag aagctgtaaa atttgaaaag aaatttattt aatcctttaa attgattcgc
    13681 atttttaata cttctgcctt ctttctccca aaaaaattta ggtggtgtca tacataaatc
    13741 tatatgctta acacctttag catgtgcaaa tttagcaaac gcaatagaga gagaaactat
    13801 aacttcatac cccttaagct taaaacttct gtaagccaat ggttggaaga gaagatatag
    13861 ctgtctaaat tcgaatttct ttggaagata ctgcatgaat gagtggtgta ttttgatatc
    13921 tccaaaaaat tctttaacaa atttcttgtc gtagtatgct gtatatacct cacagtttgg
    13981 aaaagctttg atgatgtatc taaggtgtac ctctgcacca ccatgtttaa aaagtggctc
    14041 tgctactact gctaccttaa gggtttcttc tggaatattt ctttttttct tccttggaga
    14101 catttattaa gaattgttca agattattta agaactttgt ttctgtaaat ttctctgctt
    14161 gagagattat attttgttca ttatatcttc ttttgttaaa attcttaagt aaattagata
    14221 attctttttc atccttaaag aattctcctg ttacaccctc tattactgtt tctaatactc
    14281 ctccctcttt atatgctaaa actggtgtac cgtatgacat tgcttcaaca ggtaccaatc
    14341 caaagtcttc cactccacag aagagtaata cctctgcctc tttgaagaaa gcgtttcttt
    14401 ctgcatctgg tacaaaaccc aagaaggtaa ttcttttgtc tctttttgca atctttttta
    14461 aatatctcag atctggtcct tctccaacta ttacgagatt tgtattggtt tctttacagg
    14521 cattgattgc ccagtctatc cttttgtagt cgtagagtct ggataccacc agtacaaact
    14581 tcttagggag agagtatttc tttcttacat ctattttctt gaaattttcc tttactattc
    14641 ctggatatat tacagttgaa tctttgccgt atgttttctt tatcttttga gcaacatatt
    14701 tggaatttgc agtccagtaa tctactcttc tagatatact ccaatcccat atcctaaggt
    14761 gtgtgtttaa tacccttgag aggggtctgt agagccatct aagcttcgaa cctctcacat
    14821 tcagctcatt gtcccacaag cttcttggag gggtcattac cattgctaca tggggttggt
    14881 atatttcagt aattattcct ttcgcaggtc ctgcagatat gctaattaca aggtcatatc
    14941 cgtcgaaatt gaaactttca tatgccagtg gtgtaaatat tttaagatgt cttgaaaatc
    15001 cttttggtaa aaatttcgca atattttgta caaagctggt atatacacga tttgtaaggc
    15061 ctggatattt ctttttgtca aaaaggatgg taaagatatc tgcatgaggg agtattttta
    15121 acattgagag aagttcagcg tctgcacctc catacgaagt catttgatct gctacgattg
    15181 ctaccctggg aaaaagtttt tctagtttat acatgagcca ttatagctcg gggatgtcct
    15241 ctccatcaaa tccgaaatct tctgcagaaa catcttcttc ttcagagtct aactgctcaa
    15301 gagcttttga aagttcttca tctatgggaa cctctgcctt tggggaatcg tcatggtcat
    15361 catctggaaa gataacatct aaactttcac catcatcttg acttgggtct atacctccgt
    15421 caccaatgat atcttcgtct ccacccataa gtatgatatt tggttcttcc ttgtctatgg
    15481 atacatactc ctcttcttca cttccatttt tcttcggtag aatagctttc tcaaggtatc
    15541 tatctgcttc ttcaccaaat ttctcaacaa catctataac tttcattggt tttccacact
    15601 ccggacatat cttaatctca tctacctctt ctccacattc tgcacatgag aagaaaacaa
    15661 catggacact cttcctttgt ggtattagtt catccttctt cttttttgcg ggatttgcca
    15721 ttggtgtaat aataaattgg tactattgaa tagtatattg tttagcctaa aagtgtcaat
    15781 aaacccatag agatactatt ttagccatat ttagggaagc aacacttctt gtattttttc
    15841 ccgctaccac aaggacaggg gtcgttacga ccaactttct tgttcttttt aatagttttt
    15901 tgctttccta catttcctgg ccttttgtct tcatctgtat taactctcac aggtatctgc
    15961 tcctctcttt ttactagtct taccttaaag aatcttgtta ctatggattc atctactttc
    16021 tttagcatat catcgaacat tctaaatcct tcatttttgt actctacaag aggatctctt
    16081 tgagcaaggt tacgcagaga gataccctct cgaagatcgg tcattgtatc tagatgttcc
    16141 atccagaatc tatcaaaggc ttccaaaagg agtaatcttg agagtgcttt cattggttct
    16201 actcctatgg catagatatg gagtataaag gatacaagta tgagtttttt aagttgtact
    16261 gcttgttcta cattcgtttt cttctctaaa tctttaaaca tctctactga tgtcttaaag
    16321 cccatcttct ttactgcttc ttcaaatatc tcgtctgtta gaaggttcat cacttgctgg
    16381 tacacctgtc tttcttcaac atttgatatc ttcatatcat ctacaagttg agatgcttgt
    16441 aggaaatcta tgtattcaaa ttcttttttc agaatccatt tctcaagtgg agaaaggatg
    16501 aagtttagat tatctagttc ttctattttc catgtctctt ctctgtttaa ggctagagca
    16561 tctagtgcat cttgaattcc ttctatatct gcatttagta caatgtctaa atcttttttg
    16621 agtgttttgt ctgtaacatt aaagtctgtt tttaactcaa tatctagaat ttttcttctt
    16681 agtgtatata tgatttctct ttgctggttt agaacatcgt cgtactctac aagacttttt
    16741 cggatatcgt agttgcgtgc ttctactttc ttctgtgcat tctctatagt tttaccgagt
    16801 ataccaagtg aaattggcat atcatcatcc attccaactt gggtcatcat tagctgcatt
    16861 ctgtctccac caaagagtct catgagttca tcttcaaaag atacaaagaa tctggattct
    16921 ccagggtctc cctgtcttcc agatcttcct cttagctggt tatctattct tctagattcg
    16981 tgtctttcag aaccgattac atacaaacct cccacctctt tcacaccttc tcctagtgca
    17041 atatctgtac ctcttcctgc catatttgta gctatggtaa cagagccttt ctgtccagct
    17101 tctgcgataa taattgcctc tctttcatgc tgttttgcat ttagtacatt atgtttaata
    17161 ccttctttgg agagcatgct tgaaagtatt tccgactttt ctacagatgt agtacctacg
    17221 agtatcggtt gtccttttgc atgatgttct ttaatatctt caactatggc tttaaattta
    17281 ccttcttggc ttctgtaaac aacatcattg taatccttcc ttatgattgg tttgtttgga
    17341 ggtactacaa tggtatctag tttgtaaatg gaagagaatt cttctgcttc cgtaagtgca
    17401 gttcctgtca ttcctgatag gtatttgtaa agtctgaaga aattctgaag tgtaattgtt
    17461 gccattgttc tagattctct tttaacttcc actccctctt ttgcctctat ggcttggtga
    17521 agtccttcac tgtatcttct tccttccata gctctgcctg taaattcatc gactatgagg
    17581 atttctccat tcctaatcat gtattcatca tctagttggt aaagctcttt tgctttaagt
    17641 gcattgtcta aatggtatgt aagttgtgca tcttcataga tatttttaac tccaagagct
    17701 ttctctactg catctatacc ctcatctgta agagatactg catgggcttt ttcatctaca
    17761 gcataatgtt tgtctttctg aagtgagttc gcaagagaag caaatctttt gtaaagatcg
    17821 tttgcacctt gagcactagt agatatgatt aatggtgttc tagcttcatc tatgaggatg
    17881 gaatctgctt catctacaat tacaaaatat tttggtcctt ggactctttc ttctttcgtt
    17941 cttgccatgt tgtctcttag atagtcaaaa ccaaattcat tgtttgtacc gtaaacaaca
    18001 tcacaaccgt atgcctcctg tttagaacac tctaagagat tcactccact catatggtcg
    18061 tatttcaatc tcccagaagc ttttactttc tcatctcttt cttttattaa tttttttgca
    18121 tcgtctccct tgattttaag tgcttcttcg tctgttatga atctgtactg tttaccagaa
    18181 ttgatagctc ctacactcat tccaagtgca ttgagtatgt gtcctgtcca ttctgcatct
    18241 cttcttgcga gatagtcgtt tacggtaact aaatgtgcac ctctcccagt tagaccgtaa
    18301 agatacaagg ggagatttgc tgcaagggtt ttaccctctc cagtgaagag ttctgcgatt
    18361 ttgttgtcaa agagaacata gccagcagta agctgtacat cgaaatgtct gtggtttgca
    18421 actcttttgg aggcttctct tacaactgca aatgcttctg ggagtatttc atacaatctt
    18481 cttttctctc tttccaactc ttctacaagg acggattctt ttttgttaac aaagtctgtt
    18541 ctgcaggttt ctaaatctac aaatatcttt tttctaaatt cctctgtttt agctcttagc
    18601 tcctcatcgg aaagtttaga tatttcatct tcatacccat taatcttctc tactaatggt
    18661 tgaatctttt taagttgttt ttcgtttgag tcaaaaagac cctcaaggaa tttaatcatt
    18721 gaaattgatt ataccatcat tcacagattt ttcatcttca aaatatatat ttttggtatg
    18781 tacaagatat tgttctcctt tctttttttc ttcctttcta caacggcttg ttctgcagaa
    18841 cttctcctca tatctccaca aatagatgaa tacctaacca acagggttgt tactttcaaa
    18901 tggaagaaca cagatacaca gaatgaatat gtttacaggt tggatattgc tttctacctt
    18961 aattggtcaa catatatgtt aattcctgtt gaaagcaata caacgtacac tcagactctt
    19021 tccaattacg atgtttttgc ctggagagtt ctgtatgcta gaaaaaacac ttttgatggt
    19081 acatatccac atgtttctga gactcgtaga ttcagtatta atacagatct tccacaggag
    19141 gagattcctg aaccaagacc ggagcctgag ccagagtcta aacctaaaga agaggaaaaa
    19201 cccaaaccaa ttgaaaaacc aaaagagaag attaaaccaa ctaaaaaggt tgtagaaatg
    19261 cctaagaatg aggaagaaca tatggagtgg gatatctcca ctgcctctgt tttgggcgca
    19321 aacaagaaag aggttgtgtg tcgatttaaa tatttaaagg gtaagagtga gatagtgtcc
    19381 tgtaaaattc catctctcca actacaagag attactcagt atccctttgt agatgaatac
    19441 agtgtagttg taaagggtaa atttctagat gacatctcca ttgttgtaga tgaataccga
    19501 tgtaagagag atattcttag accaatctct ttgtttaaat gcgaagagca actagtttcc
    19561 acccatgata ttactgttga accgaatatc ttcttcaata tatatcaagg agatactttt
    19621 gttccaatta aatcctacac aagggatggt gaacaattta cacttgttgg aggatatttc
    19681 cgtaaagagg agaatctgag aatgaggttt agatattctg tgaatatatc ccagtttaat
    19741 atctcaattt ccggtggagg ctcctactct ctgaatccaa agaatatttc taactcaaag
    19801 aaacctttct ctttcccttt tagcaaggta attggggtta cacagtggca tgggaatact
    19861 gcattccaaa gaccacacaa gggtatagat tttggagcaa caagagagag tgttttggct
    19921 gtttatgatg gtagcgtagt tggtaagggt tgggatagtt acaatggaaa gtgtttctct
    19981 ggtggcaact atcttctcat caagcaggac aatggtatgt acacaacata cttccattta
    20041 gaaaagactt ctgtaaacac aggagacagg gtaaaaaaag gacagattgt tggtgtaagt
    20101 gggaattctg gtgcttggaa ttgccagaag cttggttatc atctacattt cgagattagg
    20161 aaaagtagaa gtcagagtac ccatgttaat cctgtagatt acattaatac agattggagt
    20221 aaggttgtta cattaaatgc aaagtacata ccaggaaggt taagcggaga gaatccccat
    20281 cctggatatt agtttagttg tttaattatg gaatatttaa aaatgcttgt ttttcttttc
    20341 ttctctttgt tctcacagga gaaggtagaa tacatgttgc caaatattgt ggatgtgcaa
    20401 cagatagtcg aggtgactcc tcaaaaagtt ttacttgtaa acgaggggga tattttacaa
    20461 ttagatgtat tgggtagaaa ggtcaagaag gttgggaaga ggaatggtaa tgagtttgtt
    20521 ggatatgatg gtggtttgtt gttttgcaag attgagcatt acataataga tagtgaggat
    20581 gaattttcta ctaaatttac tgtgttagat gaggaaaaag atgttgtgag agaattacag
    20641 tttttcgaga ctattcgtcc tttgtatatt gataacaata taattgtagc tacaactgcg
    20701 atagatttct tggggagaca tttttacaag atatctgtag agagtggaga gatgaaggag
    20761 atatttctag aagatttagg acagaagaaa aatattacaa atgtaagaga ggatgtattt
    20821 ggtaatgtat gggttagctt cagtacaaaa aaggtagtcc ttactcatat cttaacgcat
    20881 ctatcgggtt taatcttgca gctcgagcag ctgggtatat accaaaaatt atccctataa
    20941 agcttgatat acttactgct agaagaattg aatcaaatgt aataattgca tcaaatccaa
    21001 gaaatttact agcaattaaa ctaattacat aacctgtcaa gataccaaat attcccccta
    21061 ttacagtaag aacaactgct tctgtcataa actggattaa aatattggat gtttgtgctc
    21121 ccaaggcttt cctaagccca atctcccttg ttctttcagt aacagatacc aacataatat
    21181 tcattattcc gattcctccg acaagaagtg atatagcggc tatacctgca agaaaagatg
    21241 ttagcatagt cataactgta tcaaccgtag agagtaaatc atttggggat gtgataaaaa
    21301 aatctgccaa atttttatca tcaatcttgt gagttgtaag taatacactt tctatctcgg
    21361 tctttgcatc atcaactgta tcctcagatg ttgcttttgc tatgatactg ttgtaattat
    21421 cagtaccaaa gagtttgaaa gcctcttgat ctgttatata tgcttggagg tttgggttgc
    21481 taaaattagt ttcatcttgg atttcaagga ttccacttac tttaaattta atatccatta
    21541 ttggaatatc ttgaccaatt gggttggtat ctccaaaaag atcttttgct aattgatctc
    21601 caagaacaac ttcttttcct ttagggtatt ctccggagga aagattgagg tcatagaatg
    21661 taaaatagtc agaggataca ccaataattg ttcctctttg ttcttttccg tttgttttaa
    21721 gaataacttg gctagaggca tatgctgcta tatttgatac taaatcacta gatatttttt
    21781 ctaatgctaa gaaatcttct tgggttaatg attgagaaga accaaacata gcaccatttg
    21841 cctttggtgc ttcttgtgga ttagtagtat aagagtctct ggaggacata ttgaatcctg
    21901 tcattatcgt taaatttgta ctacccaaag agtttatctg tccgtttata ctttctttaa
    21961 ctccagtccc aagggacatt aatcctatga cagaaccgat tccaattatt ataccaagta
    22021 ctgttagaaa gcttctaacc ttgttagcca ttatggcctt aaatgattgt tttaatattc
    22081 taaagaattt catctgttgt attcctcctt tacaactttt ccatcttgta tatggattat
    22141 tctagaagca tatttcgcaa tatattcctc atgggttatt agtaccattg tggaaccttt
    22201 tttatttatt tctttaaata gttccattat ttcttccgat ctttttgtgt ccaaatttcc
    22261 tgttggttca tcagctagta tgatactagg atccatcatt agtgctcttg ctattgcaac
    22321 tctttgtatt tgtcctccag agagttgatt acttttgtgt ttactccaat ttttaagtcc
    22381 taccatatct ataagggctt ctgccttttc tttaggattt gggatatttc catatattgt
    22441 tggcattaat acattgtcaa aaactgtcgt tcttgctaga aggttaaact gttggaatac
    22501 aaatcctatt tctttgtttc taatttttgc tagttctctt tcttttaatt tgctgacttt
    22561 ctgttcattg agtatatatg ttccacttgt gggtgtatct agtaacccaa ggatgttcat
    22621 gagtgttgat ttgccacttc cagagggtcc cattatggca aggaattctc cttcttggat
    22681 gtttaaatct atatcgtcaa gtgctagggt atttccacct tccgatttat atatcttaga
    22741 tatgtttttt aattcaatga tattttcgag catgaggtct attctacaac tatttagtta
    22801 acaatacgag atactgtaaa gatgattatt ccaacactta aactaaattt gaagatgatg
    22861 ctgaatatta ttcctatgaa tgttccaaaa cctgctttaa caccttgagt tgcattttta
    22921 tgtgcaaata ttatctcaaa tattgttgct ccaaatagtg gtccaagaaa catccctgct
    22981 attccaccag ctagcattcc aattaatcca ccaattattg cacctgcaat tccccaacca
    23041 cttcctccca ttttttttgc tcctagtgct gtagctatat tgtctgagag tgtagagaga
    23101 aggcagagga gaaacaggat gacaagaact aagggagtaa gattctcaaa cttgtccata
    23161 attgctagaa caacagtaga gatgtatatg agccagatac cagggagatt tatagcgaca
    23221 agtacaaggc ctgctagaga gactagagct agtaatatgt agattattat ttctaacatt
    23281 gtattatttt atcattaatt attatttaca tcatatacca tatgtggata taatacagat
    23341 gaaaagactt ggcttctgaa ctgacgcctt gtaggtgtgc gtttctagac ttgctctaga
    23401 ataggggcta ggtcgctttt tcccaaatat cctctctagt attcctaaat ctccagattc
    23461 atctatgaat ataatcatag agaggagttt aacaaagaat atctagggaa taatgaaatt
    23521 taataactat atcttctcaa atactccatg ggttatctca gcacttttct cccatgtgta
    23581 ttcttttgag agtgtgtatg ctttttctac tagttctttg tatttgtctt cattcatata
    23641 tatatcctta atcacacatc ctactaatcc tgggttgttt ccatctacca ttacagatac
    23701 atctgagaag agctctagat atactggact tctgtgtagt atggttggag ttttagatct
    23761 cattgcttct attggtgcta tgccaaatcc ttctgcaagt gagaggtgta tgaatgcgaa
    23821 tgagttatgt agtagagagt atttgtcttc atctttataa aaaccagtaa atattacttt
    23881 gtcttgtata ttgtttttct caataatgtc atagagttct ttaatttctg gttttgtttt
    23941 gtcttcaaat ttacctcctg caattacaaa gtatggtgct tgttctttgt ctagagtttt
    24001 aagaaaggac acataggaga gtaatgctcc tttagagttc ttggtcctat taattcctgc
    24061 acccaaatat atgaggtatt ttttctcttt ccaatctttg ggaaggatat tgtctaggga
    24121 tgtctctttc tcctctattt ctacccctat gtaagataca gatattttct ctgatgggta
    24181 tttggggtaa tactttagga aatctgtttt agtgttttga gaattggtta tgatatgttt
    24241 acatttcata gctttgttta gtgtcatcca atattgtatc tttctgatga agttgtgtat
    24301 tggggattgt tgggagtaca tattgaacat gggaaggttc atatcgtgta tgaagagtac
    24361 aacgggcatc gtgtatgagg ggaagtttct ccagaagtat ggacagaagt atatgctgtc
    24421 tttttgttta atctttttaa tagcgggaag gatttgtgtg aggtaggtta tgtcgttttt
    24481 gtagtcgttg tctttgtatt tccctattct aattatttgt gcatttggat atttggatat
    24541 ttctgtatct agagttgatt ctttgtcttc aaaaaggagt atggagtatt ttgtatctgg
    24601 atattcttta acaagagaaa gaattatatt cctggtatat ctgcctggtc ctgcatatgc
    24661 catttggtct tgggttgtag ttgcatctat tatgatgtgc atttatttaa aactataaat
    24721 taaaacattt acaaaaaatg tcttgatgaa tactccgtaa attctaatga ctccataaag
    24781 tttatacttc cacggtgaat acatcaaagt aattaatgtg ctactccata aattatttga
    24841 agatcttctg gaaaaatata attgaaagaa gtctcggaat gtaagtttac tttgtgcttt
    24901 cactcttcgc gagagattag gcctgctatt tgttgaaatc gtttcagtct tagtcgttac
    24961 tccgagtttg aatccatggt tttttgccct acagaaaaaa tcataatctg acgcatactg
    25021 aggaaagatt ttagaaaaat tcccaatttt gtgaaaaacc tcaattggaa ttaatgctcc
    25081 tctcccactt aatgtatctg ctgaaattat ttcttctttt gaatctatta tcactggtct
    25141 aaatgatcca tggattattt tatgtgagtc atggattaat tttcttgata cagaatcgat
    25201 gactggtgtt ccaagtatta ttctattatt ttcttggctc tgtttaacca atttttcaag
    25261 gaaatcttct tccatatatg tatcatcgtt ctgtattaga acaaaatctg ttgcctttga
    25321 tttcggcaat acatactcta cacccatcct cattgctcca ctccaccaaa gattcccatc
    25381 accctctaga actgttacct tctttttttt caagatatcc ccagttccat ctgtacttcc
    25441 atcatctatt acaacaattt cgatatcgct ataactttgt tttaagagag aatcaagaca
    25501 cttgattaga agatcttttc tatttctggt aggtatgaga acaaatactt tcatacaaat
    25561 atattacttt tgaagtataa ataaaccaag agaataccac ttatcctttt gccaaatggt
    25621 ctctgcagct tctttctctg atacagttaa ccatatccca tatttcttgt tatatttcag
    25681 atattcttct ttcaggagtt tatacccctt gaatagtttc tctgttctat ttttattata
    25741 aagtctgtta attggtaaaa catcctgtgc cccatatggt actgtaagaa aaagtttgcc
    25801 tcctggcttt aatatcctat acatttcttt cattgccttc atatctccag tttcatctcc
    25861 ttcaacacca tatcttcctt cgacaccgat atgttccaag gtagaaacac aggtaataca
    25921 atcaaagaga tttgatttga aatcagtttt tcgtatatca ccctcaatct gctttacccc
    25981 ttttacatct ttttgactat tgatattcaa agtattaatt tcgactgttt tggggaggat
    26041 atcgtacaac atttgcccaa cagtagaacc aatatctaaa agtttaccct tcttaatatc
    26101 aatattcttt aatacccatg gatattcaaa atctctgtca aaacattttg gggctctgtt
    26161 cctcagtttt gccaatttga tattatcttt aagagattgg cattcatttt gtttaatctg
    26221 cattcttaat acttgaaatg gaaattcaat tatcttcgta aacttgttcg ttatctgatt
    26281 caatagcatg atgaggtatt tttaatttat atcttttaat ttcgaattcc agagttttac
    26341 tattttatca tgtgtacttt cttgggccat cttctttatt gattcgtatg cttctttgga
    26401 gatttccctg tactcagttt catcttttaa caaggaaata actgcgtcag cataatcttt
    26461 tggatttctt gtattaatta gacgaccgtt tataccatcc gtaactaaat cggagatatc
    26521 tcctacatct ggaagtacac atggtacttg tatggacata gcttctacag ctaccatggg
    26581 aagaccctca ctctttgaag tcatcaataa aatcttagat ttatcaatat atggataaat
    26641 atctgatgtt ctaccaacta acttaatatc ttccttaagt cccttttctt ctatcatttt
    26701 ctctatcatt cccattctta ctccatctcc aagcattaca ccacttacag tccctcgtat
    26761 cttttttact tcttctgcaa catctaccca caaaggaaga tctttctcat ctat
//
LOCUS       DAOQWP010000009        26639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_2130_length_26639_cov_7.388429, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000009 DAOQWP010000000
VERSION     DAOQWP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 26639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26639
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2130_length_26639_cov_7.388429"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..479
                     /locus_tag="PKI16_01790"
     CDS             45..479
                     /locus_tag="PKI16_01790"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HNR53188.1"
                     /translation="MKLSGRTIFIGLATLIIVGCSISYYFVTNTKPNALGWTSSSWLY
                     KKSISIPNKSHMLINKTVSIELDTESLIRTGKLQNDCDDLRFLDDDNQTSLQYWIEGE
                     CNTTKTKVLIKIPSLPHNGKTIYVLYGNPTAFNLQEKLTINE"
     gene            472..1158
                     /locus_tag="PKI16_01795"
     CDS             472..1158
                     /locus_tag="PKI16_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53189.1"
                     /translation="MNNHSKLIQKMRNKYSLLQLLFLLIAFFILSPSIVKATELDKYK
                     DSNTESPYYAPILFQAHGQKLNEATQEIQFTVTSVSVNNEIIDITENKDVYVTKDDAV
                     RIVGKTEPKTKLTVYYADKKITVTARENGDWIVLFSITNMSESKYPVSVQLDNAKKTT
                     SLITLVVGSGRKIIQPVLDTSISQGSSFFQDKGEYLAFIFVALLATILGWFLGSYSEK
                     KKRNKNNRMK"
     gene            1162..2889
                     /locus_tag="PKI16_01800"
     CDS             1162..2889
                     /locus_tag="PKI16_01800"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53190.1"
                     /translation="MLNININKKQQLYILISNVVLLSFFGFLTITPINNVEACSGNCP
                     TGCISHYICGCAGVGGCVYRETCQSNEFRCSTDMCCDICDGSCGSCTCTPPGCPTGYS
                     TTNTGCSTTTTECTKYYEDSCCGSCGTKERTCWLLKYTLKYTAGSNGSITGTTSQSIC
                     RNSSGSAVTAVPNSGYHFVNWSDSSTSNPRTDTNVTANISVTANFALSNEDPTAPTSL
                     LTEGLTNPTGIGDTTPEFSAIFNDPNTGDTGNYYQIQVNTASDFTGTSMWDSSLTAMT
                     ATAIGARSPDISYAGTTLTLNGTTYYWRIRFADNHAATSPWSSTANFKMNSIPTAPTS
                     LLTEGLTNPTGIGDTTPEFSAIFNDPNTGDTGNYYQIQVNTASDFTGTSMWDSSLTAM
                     TATAIGARSPDISYAGTTLTLNGTTYYWRIRFADNHNETGSWSSTGSFVMDGAPFAPT
                     ALLTDGMTNPTRIFSTTPSFSAIYTDPNGDDADIYQIQVNTNNTFTGTVMWDSGQQAT
                     STPDGQRSEEITYAGTPLTNQNTTYYWRIKFWDLEDYEGPWSATAQFSSLQASFLFEG
                     VKIGDLKLD"
     gene            2908..3654
                     /locus_tag="PKI16_01805"
     CDS             2908..3654
                     /locus_tag="PKI16_01805"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNR53191.1"
                     /translation="MSLKIESFKKLRKDKRKWKVDTPEEFEEREDSFLKKRLMSKEPT
                     IDTFVRISGGKAKNFKLEIPKTTRPLTDRMKIKIFDILREDIVNKDILDLYAGAGSFG
                     LEALSRGAKSATFVDASKNAYFVLQKNIAHTGYLPESEVIKSKTEDYLFKQTNQKKKP
                     KFDIVFMDPPYKLFNTKNIIKMERVMKMASQLIIGIQNPKTNRFKGALIVKHPRRYPI
                     EKITIDGMKIIETYEIGLNCISIYIVDLKR"
     gene            3805..5580
                     /gene="thrS"
                     /locus_tag="PKI16_01810"
     CDS             3805..5580
                     /gene="thrS"
                     /locus_tag="PKI16_01810"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNR53192.1"
                     /translation="MNSDVEEISKIEKIRHSASHVLAQAVLKLYPDAKLGIGPAIDNG
                     FYYDFEFSEPIEETDLPKIEKEMKQIIKQDLPIKQIFMKRKEAQEYLEKTDQTYKLQL
                     LEEIPDKELSFYITGVNDFADLCRGPHVESTKEIGFVKLTKTAGAYWKGSEKEKMLTR
                     IYGTAFETKEELKDALAKMQLAEQRNHRKLGKQLELFAIIPEIGQGLPVWLPRGYKIR
                     RILEEYMLDLEEEAGYLHILTPHINKEILFKTSGHLDFYKDSMYPPIVIDDETYYLKP
                     MNCPAGMMVYNLSPKSYRDLPYKLGEFGTVYRYEKTGELHGLQRVRGFTQNDAHIFCA
                     PDQLDDVLNETLDLLDIFYKDVGFDNYKFRLSLSDWENKSDKYAGDPKRWEFAEQTLR
                     QVLNNRGLEFEEMPGDAAFYGPKIDVQAVNVFGKEDSISTIQLDFNLPEKFDIKYIDE
                     DGVEKQPYIIHRALIGSFERFFAFLIEHHGGDFPLWLAPEQVYIVPISEKHNDYAKQV
                     YASIKEAHLRVKIDDRNKSMQSKIRDSEKMKIPYILILGDKELDTQTVSIRARKSNEH
                     GLMKTQEFIDTLKEEVRTKGNPSEN"
     gene            5601..6212
                     /gene="infC"
                     /locus_tag="PKI16_01815"
     CDS             5601..6212
                     /gene="infC"
                     /locus_tag="PKI16_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNR53193.1"
                     /translation="MSYGEKREFKKDYGPRVNEYIRVPQVQLIDEKGENLGVVDTEQA
                     RKLALEAELDLVEVGSSAKPPVCRIMNFSKYVYEQNKKKRQNKGGKMKEQKEFRFTPV
                     MDIGDTDYRIKRAHEFLTKGHQVKLTMVKKGRQSMDQARSVFTEILTNFADYSSIEAE
                     PKSEGNRISITFKSDGKTKNKQNSEEKNQAIQPEGEQKAKIKI"
     gene            6347..6685
                     /gene="rplT"
                     /locus_tag="PKI16_01820"
     CDS             6347..6685
                     /gene="rplT"
                     /locus_tag="PKI16_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNR53194.1"
                     /translation="MRVKGGKVRSRKHNKVLASTKGYRMTKSNLYKVAHEAYLHAGQY
                     AYNHRQRKASEMRRVWISRINAACKNNNIQYKDLIDGLKKKGSTLNRMVLADIAYNNP
                     EVFSFIVKSL"
     gene            6767..7795
                     /gene="pheS"
                     /locus_tag="PKI16_01825"
     CDS             6767..7795
                     /gene="pheS"
                     /locus_tag="PKI16_01825"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNR53195.1"
                     /translation="MHTLKTLEKIKTEILESNSSYSEFKEKYLGTKGLVTLALKDITT
                     IDKKDRAEYGKQINEIKNICNDILQKKKLQEEKIHTSKKIDPTAPFDINTPSDKLPEI
                     LDLKGSKHPLHKEVEIFTDIFKDMGFDILESRQIDDDYHMFESLNFPKGHPARDMYDT
                     FWTEDGFVLPAHTSTMQNRALKKFGPPPIRVVLPGRCFRNEATDASHEHTFYQIEGIY
                     VDKGISLANMQATIKTFLEAYLEKELEVKIQPAFFPFTEPDCEFIISCPFCDKVGCST
                     CNHSGWIELMGCGMIHPNVLKQGGINPDIYSGFAWGFGVDRLTMIKLGIKDIRLLRDS
                     NLNFLRQF"
     gene            7798..10287
                     /gene="pheT"
                     /locus_tag="PKI16_01830"
     CDS             7798..10287
                     /gene="pheT"
                     /locus_tag="PKI16_01830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNR53196.1"
                     /translation="MKAPINLLKKLSGVKLDDKEIVRLISEHIGQVEDFHDLRGDYKG
                     ILVAEIKEKQDHPNADKLAIYKISTGDKKLIQVVAGDKTLEVGDKVAYLRPGSKIPYT
                     IYTEAEPVIISKRKIRGVESNGMLGAEKELNLGSDYTKVMRLPKDAPIGEDFAKYYEL
                     DDTIVDIENKALTNRGDLFGILGIARELSAITNNKFESPQWYLDNDKKYTEETSCLNI
                     EIKNDAEVICPRYTAISFDNINIQESLIWLKSFLVKSGIASINNVVDITNYVAHLVGQ
                     PLHAFDYDKLVNNDLKAKGTARINIRMAKIGETILALDNKIYELDENIMVIADSTHPI
                     AIAGIIGGKDTEVDANTKRIVLECANFDKTAIRKTSMRLGISTEAATKFKHAISPDTC
                     IPTLNKAIELIRELTEASIASDIVDIYSLPQRTKSLSFSLASLNTHLGTDLDKETVEK
                     ILSNMEYGMEKEDEGYVKVSIPYWRMDIDIKEDIFEDVGRIYGYNNIKLELPTRKIKP
                     SGINKLYNLKRQIREILSNSGANETDTYSFTDVQTLKKCNQDPEFAFRIKNALAPELS
                     LMRTSLISSLLTKTEENIQRGFNQFALFEMNIAHQRQLVDGNGLPIESWSLAMVICDA
                     TNNSQTSAYYLAKRYIAKIFSTLHIEGIGYSLISESSEKELPINIKNLIYSFESNRTA
                     LIKYNDIVLGVIGEFNSVVVNNFQLPAYSSGLEIDLNALLNVEQKSKQYREMPKYPAS
                     INDVCIEVNKDTQYIDLENEIKKIVNKDKLWGEVSCIDIYSKGKEDSKKRITFRLTLR
                     HNSKTFNSNDISSIICNIEKNIREKFDATVI"
     gene            complement(10293..13001)
                     /locus_tag="PKI16_01835"
     CDS             complement(10293..13001)
                     /locus_tag="PKI16_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNR53197.1"
                     /translation="MRYSVSVTNSPGKSVGSNSLRRMDFARYNNFKKYNLSNRKKVKG
                     EKSTDLSGKKLLFKKKLKKALYILVGIGFFLGCLVLLGLGLYLKNLQKSLPSPDELVE
                     RTSDQSTQIFDRNGKLLYTVYGDQNREFVSIDKIQEHTKWALLAAEDIEFYQHKGVDY
                     MGIAKAVIQNFTAGSVVRGASTITQQLVKTTILYDVLGDEAYKQTYSRKIKEILITMQ
                     VEQTFSKDEILQMYMNEIPLGGVNYGFEAAANAYFGKNVEDLDLAESALIAGLIQSPG
                     VYSPLYGTNPDLAKVRQTYVLDQMLKHKNLTGVTEEEIQAAKDQELVYNTKRIDIKAP
                     HFVFYVKSLLEEEFGVDRVERGGLKVTTSLDSSIQQIAEEEIAKGVDGAKRYNVHNGS
                     MVVIDPKTGELLAMVGSVDYWNVKDPRVDGNVNIALSNRPMGSSVKPFVYLTAITKGY
                     GPWMVAPDLSEIKFGTYDPKNSDSKYMGLITSRKAIVESRNVSAVYNLQLVGIDAFLQ
                     TMQKVGVTNFSNKESYGLSLALGSGEEKLLEHTAGYMVFATGGIKKDVISILKVEDSK
                     GEVLQEAGENKGQRVFDEKEIYLLNWMLCDLGGMNDRPNNSKYTVNKTRVCGKTGTTD
                     GPKDLTTILYHPNLIVGVWNGNNNNQVLPGAWGSTVPLGIANSFMTRVAGKYKPTTYN
                     RPAGVLSTTVCTDTGATPADGVDCKKEASVYISGRAPQVDNRKAYEICKANGLIPENL
                     EAARKYGLTTTKIVLSTTLENQYQRDAYTKYLLSMKNSNYLPKAPATGICPLPLGPDN
                     APLIELTKPISGQKIVKGNNLEITGSVSVMESIATFEAKFDGNAILGATYNPNGTFVI
                     DYFVDISTPLGDHTVSITVVDNKGKSSTKSVTINVVKQEPEPIIIP"
     gene            13117..13809
                     /locus_tag="PKI16_01840"
     CDS             13117..13809
                     /locus_tag="PKI16_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNR53198.1"
                     /translation="MKNLRQTRFLLISNSSLFKQLIRDNLHLQGSTLSCNTVISAKGS
                     LDTELFSAIIIDQDIQSQDSTAYSLLLFSIYQKDLSDSVIIFSKEISSETIYMYYKYR
                     IQNIFFLSSVNLFLEPVIRRILSLIPETDGMVLRDRGISLYLSNNYAIFKGCRILLSK
                     TEKLILAYLLKKCSLCTKTELINYLSNTLQREISEGYLTVNISRLRKKFVKHTGINIV
                     KSRNGFGYYLSV"
     gene            complement(13806..16409)
                     /gene="mutS"
                     /locus_tag="PKI16_01845"
     CDS             complement(13806..16409)
                     /gene="mutS"
                     /locus_tag="PKI16_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNR53199.1"
                     /translation="MENTPMMRQYQGFKKQYPDKILLYRMGDFFETFGEDAKIASKVL
                     NITLTTRDKKNNPTPLAGFPHKAIDQYLPKLIEGGYCVVVVDQLEDPKFAKGIVKRGI
                     TRIVTPGTLDGEKAKSSKNQYLAAFSKEKTLTSVALCDVSTGEFLLISESNKKDIANR
                     ILTSYDPEEILLVEGEESFRFDDKPVHFVSKGLKNKQYSSEVCKDFFDLKSIESLGIE
                     GESLDIVSVAMILEYIKETQLMSPKHIQKPRKVSIDNTMVLDSATIRNLELIRNAYSG
                     DISNSLLSVLDNTCTPMGKRLLFSWILNPLLDKKRIEERLEKVEKLFTKKSELEKIRE
                     RLSEIGDVERIVGKIGLGRVNARDFNYLQTSLKNFLEIADILKKEFDISFDIDKKLIE
                     GLISKIEKTISDSPPLSLLDGDILKTGFNQEVDELRSLSGDSKTWLEEFEKKEKKDTG
                     ISSLKISFNKVFGYYIEVTNAHKEKVPERYIRKQTLVNAERYITEELKEKENIILNAQ
                     EKVCSLEYQLFCEFRDSFFPVLGELQKCSQYLSNIDVLSNFAHIAHKNNYCRPEIYEM
                     GEKDGVINIEKGRHPIIENITESEFISNDTQLDSKNNRMCILTGPNMSGKSTYIRQVA
                     VIVLMAHIGSFVPAKEASISLVDRIFTRVGASDDLARGRSTFMVEMDEAANIVNNATK
                     YSLIVLDEIGRGTSTYDGVSIAWALAEYLISETQARTLFATHYHELLKLEEKYPDAVK
                     NYNVMVEENLEEGTVIFLRKIIAGGTDRSYGIYVAKMAGLPNKIIKRANEILESFEQE
                     SMFVKKNELRDINVLNSSKNIPRNGNSGLQYPLFTAKDSEIEKEIQSLDLDNLTPIEA
                     LNKIVGWKKKI"
     gene            complement(16453..18996)
                     /locus_tag="PKI16_01850"
     CDS             complement(16453..18996)
                     /locus_tag="PKI16_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNR53200.1"
                     /translation="MDNSDAKKSNVFFFEDFSSKDLNENQVGRKGLSLFQLKDMDVPV
                     PDFFVISSKVFLDFSVKALASEEKKLLENGRNPEGEEVADVFLKNDLGKEIEEEILSA
                     YTRLSGFSDAWVSVRSSVVFPENKEVSFSGIFSTELNVRHFSDLLSAVKRIYASMFTD
                     DVVAYASKMDVDLSEVKLAIVIQKMVQSEISGVVFTIDPITQDEGKLSIEAVYGLGDV
                     ISLGEITPDSYTLNKRDLSIVEKHIAPQEWMKIRTMNQGRGRRGNEEKIKISNSWSHR
                     QKLNDKDMQEISKIALIIENKSREVQNVEWVISGGKVWVLQNKPLFGEHRDRIVVGDM
                     NMAKRNLREVIVALLERYRGEEMLVSQAMSEAQRIVQRSSNEVGEKFEKLVGIAKQKT
                     EEARENKTNIEDFVLSGIGASFGVATGKVSIVEGFVNKQFNNKDILLVKKYSSEMESM
                     IISCGGVIMETGGLTSDTAILCRECKIPAIVGAQGASEILKDGDMVKLDGNAGSIYRQ
                     VEEKEEALHPVVEAYGDGNVVSVDLLEKKEEVKVEEEKPGLVIPRDSTLSPSATKVFS
                     MCTIPAKDMLNTVGNSNGIVYVDLDEIMLQDGRHILSYVEQKKFVDYSTKICEKVMEY
                     VDLAQGDEVVLSIGSGTVEKFKALVGGKEFENSVLSDEVFGGTHYINNLEMFRRVLKI
                     VKRIRNVYKKRNVSLGIHAPMNEDIMKELKKILLGEKLRRSSSFSIYAILDNPSEVIL
                     ADEIVNTKIDGLILNMPRIAKQMQGIKLDAKNAKYDLGRNSVFKIVENILEVVKNKET
                     KIIAIVEDSKPLLRFCVQSGVYGVCVLPEDIVEARKVVSEEEAKLILGK"
     gene            complement(19033..20193)
                     /locus_tag="PKI16_01855"
     CDS             complement(19033..20193)
                     /locus_tag="PKI16_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNR53201.1"
                     /translation="MKKIFSFGDNSKELKEETRDRKELADVLEYQRKEGDRRPLVVVE
                     FSWKIVIFFVLLLAVIFWGKQLSTVAIFVFLGFVFMTAAKPVVDWLTSKKISKGWAVL
                     ITYLLGILVFLGLFSIVIIPFVSQLAEFVAVLPTWISQEIANFKGISFAGFSIDASTV
                     STYVTDFFKNLPTADGFKNITDALSSFFSTTTLVITSLIFSIYMVLEYDSLLEFGLIR
                     IAADEKRNRVKKLFVDVQKKLGRWLLGQLTVSTIAGIVALIILTIVKVPFALPLAVFI
                     ALLDAIPNIGATIGLVPAIIVSLITVGPVGTIIVVIASIIYQQIENNFIVPKIMGNAV
                     GIKPVIVMLGVVSALLLFGIFGALIAVPVMVLAKIAYEFYIDLQKLKAKGIV"
     gene            complement(20290..21225)
                     /locus_tag="PKI16_01860"
     CDS             complement(20290..21225)
                     /locus_tag="PKI16_01860"
                     /inference="COORDINATES: protein motif:HMM:NF037006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNR53202.1"
                     /translation="MAKKKSKSSIQTASKNIPQKKESMVSKIEIKKPQDQERVSRIVG
                     TIFIVLGVLLISFGIYSFIKYNRNPEINTTYEAPVIVGVNTLTNQGSVVIRGSASGYD
                     QVFIYVDGEEVARTKVTKDGSYEYTVDLVDEGKYAVTVAGVKGFPRRYISPISEVLNV
                     TVDKTAPTISKIKYPAEVGTETFSLTGVVEPNAVVTVKRGTGLYRATCDEKGNFKISG
                     IVLEKGPNVFNIELKDEAGNETKVEDKIRVIYSEDSDVNGEAVDDSSIPVAAGELSDA
                     MSTLLGNKLMIIFGILALIGMFSSTGILLYKNRKA"
     gene            21307..21993
                     /locus_tag="PKI16_01865"
     CDS             21307..21993
                     /locus_tag="PKI16_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HNR53203.1"
                     /translation="MNKGSKIIITVLLLLLALVPLVYFLVYPVYNDILMKKNSLDMYR
                     FSKQISINLLTQISPSADVFSKEILGVDSMEESLKSENLVLKDTLLEDMKTKVIIDSA
                     NIEGYIAQGLRSETMMRGFWHFPTSVYPGELGNSVIIAHRFQYLPPAKNTFFNLDKVR
                     VGDNVILQGESGEYTYIVTNIKEVDPNDTSVLMQTDDYRVTLITCTPLWTNHKRLVVT
                     AKLDKLYKKV"
     gene            22032..23069
                     /locus_tag="PKI16_01870"
     CDS             22032..23069
                     /locus_tag="PKI16_01870"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNR53204.1"
                     /translation="MEQFYERKEISKDTVELKITIPKDSFKDSYNILLKDHLKDTSVK
                     GFRKGKVPTDLLESDLKGELKIETLDKLAPLYINTAIQKENIEPIAPPTYKELPKLED
                     DKDVVFTIEITVLPEFKLGNMKKIKIEKEEVKITKAEIDTALEEIKKNRETKTKEVND
                     KWAVEIAEMLKIEKVKDLESLKKYIKEALKKQKEHMFLHKREEEALVQAISLSKIEIP
                     LPAVKFEASERERSFIYDMQQRGVKPEEFMKAQNITIEKMREMWEKDATEALETDVFL
                     KVYKKENKISVSEIDLNERIDEIKKSAPKGRDTSVYDDPQWREYVQSVYEKEKAFDHF
                     RKEVLGDIHKD"
     gene            23087..23668
                     /locus_tag="PKI16_01875"
     CDS             23087..23668
                     /locus_tag="PKI16_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNR53205.1"
                     /translation="MYLIPTVIEKDRDGERAYDIYSRLLKDRILFVTGPIETEMANTV
                     VAQLLFLEKENPKEDIQMFINSQGGQITAGMAILDTMNYIKCEISTIAVGLAASMGTV
                     LLANGTKGKRFALPNSKIHIHQPIGGVEGQASDIAIEAEEILKTKKLLYEFLSEKTGK
                     SMKQIEKDADRDKYFTAKEAKEYGLIDKVIKNR"
     gene            23668..24183
                     /locus_tag="PKI16_01880"
     CDS             23668..24183
                     /locus_tag="PKI16_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53206.1"
                     /translation="MALDDLTGVEDQRKQDPLDSILFNINQMYEYGTDADTVSLDLDN
                     VSLQDVDRYLKKVERDQDTPIKLFTVNFAGLYSPNEAPQIIDEAFVNLAEYPNIQNVY
                     FLEGGKFALALFPIIVENKKLCVIIQPLEQSLPFVKREYYKVVEQRHNKRKTVEELKE
                     QISFVKFPKQK"
     gene            complement(24170..24583)
                     /locus_tag="PKI16_01885"
     CDS             complement(24170..24583)
                     /locus_tag="PKI16_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HNR53207.1"
                     /translation="MITKEELKYIAQLFSDYMIKEINNDPGYIVKDEGRLESSLAQPF
                     QSIEGKELYPTLVDKGSMLYYFCIKNHPFEDGNKRMGIFSLLLYLYKNGYWLDATNDE
                     LFDITVYTASSKVKDMDKVVERIKKFVQEHLVTSV"
     gene            24738..24814
                     /locus_tag="PKI16_01890"
     tRNA            24738..24814
                     /locus_tag="PKI16_01890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24772..24774,aa:Val,seq:gac)
     gene            complement(24785..26578)
                     /locus_tag="PKI16_01895"
     CDS             complement(24785..26578)
                     /locus_tag="PKI16_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNR53208.1"
                     /translation="MEEEKVKKKVALYIRVSTEEQVEMYGVDLQRSSLMGLIASKGNN
                     LEFAGEQYVYIDEGVSGTTEPMDRPEFRRLLEDISRSTKRPFDVVAVYKIDRFARRLK
                     ILLEILDMFDKGEEKIEFLSANESIDTSTPFGRAMLGIIGVIAELEMENIKERTSSGR
                     KASAEQGTYMNTPPLGYIKGNDGKILIQEEERKVVELIFNMFVREGRNVNDIARYLKE
                     HKIMTPQAYRYKHNKEEQKGRKLSAGGPYAWDPSAVRNILTNELYIGIQYYNKTVDKK
                     KVPKDKWGVYYHDQDFIDKELFKKAQEYISASTTRYKRVQKESRLYLLQGLLKCANCY
                     NPTIHTEPYAWNGFPKKVKSTGEKVYYYQCSSKNSNKKERRNIDCKTIPLPALPLEKH
                     VIEFVRELLNNPKVVFNYQQSLQSKKADVRAKKDRLKIVKQLLNSTESAKRRIEIMYR
                     DGDYTRERKIKEIDELDAQYGRNIKEKEILESELSIFSNTEEYFKAFELFQEKYEEAM
                     KDYLDNKNIDYLYGLIHMMIHEIIVFSRPKRKSDSISGPKKEGQMIPYKLKIVLKIPS
                     EMLGDLLFSLSPESKLQAEKDGWWAIRDSNL"
BASE COUNT         9054 a         4971 c         4326 g         8288 t
ORIGIN      
        1 tgagattaca agaaaaagtt aactttttct gctaaaatgt ggtaatgaaa ctttcaggaa
       61 gaacaatatt cattggatta gctacattaa taattgtagg ctgtagtatc agttattact
      121 ttgtaacaaa cactaaacct aatgctctag gttggacatc tagtagttgg ttgtacaaaa
      181 aatctatctc tattccaaac aagagccata tgctaattaa caaaactgtt tccatagaac
      241 ttgatacgga gtcattaata agaactggaa aattacaaaa cgactgtgat gatttaagat
      301 ttttggacga tgacaatcaa acctctctgc aatattggat agaaggagag tgcaatacta
      361 cgaaaactaa agttctaatt aagattccat ctctacctca caacggaaaa acaatttatg
      421 tactttatgg aaatccaaca gcatttaatt tacaagagaa attgacaatt aatgaataat
      481 cattcaaaat taattcaaaa aatgagaaat aaatattcac tattacaact tctttttctt
      541 ttaatagcgt tttttattct ctcaccttca atagtaaaag caacagagtt agataaatat
      601 aaagattcca acacagaatc gccatactac gcaccgatac ttttccaggc acatggacaa
      661 aagcttaatg aagctacaca agagattcaa tttactgtca cttcggtatc tgtaaataac
      721 gaaatcattg atattacaga aaataaggat gtttatgtta cgaaagatga tgccgttagg
      781 atagtgggga agactgaacc aaagactaag ctaacggtat attatgctga taaaaaaata
      841 actgtaacag ctcgtgagaa tggtgattgg attgttcttt tcagtataac aaatatgagt
      901 gaaagtaaat atccagtttc tgtccaacta gataatgcaa agaaaactac ttctttaatt
      961 actttagtgg ttggtagtgg gagaaaaata attcaaccag tcttagacac tagcatatct
     1021 caaggaagtt ctttttttca agataaagga gaatatcttg cctttatctt tgtagcattg
     1081 ctcgctacga ttttagggtg gtttcttggc tcatatagtg aaaagaagaa acgaaataaa
     1141 aacaacagaa tgaaataaga aatgttgaat atcaatataa ataaaaaaca acagctatat
     1201 atactgatat caaatgtcgt tcttctttct ttcttcggat ttctaaccat aacacccatt
     1261 aataatgtag aagcttgttc aggcaactgt cctacaggct gcatttcaca ctatatttgc
     1321 ggttgtgcag gagtaggtgg atgtgtatat agggaaacat gtcaaagtaa tgaatttaga
     1381 tgtagcacag atatgtgctg tgacatttgt gatggttcat gtggttcatg tacatgtact
     1441 cctcctggtt gtccaactgg ttattctact acaaatactg gatgttcaac tacaaccaca
     1501 gaatgtacaa aatattacga agactcttgc tgtggatcat gtggcacaaa agaaagaaca
     1561 tgttggctat taaaatatac ccttaaatac acagcaggaa gtaatggctc aataacaggg
     1621 acaacatcac aatcaatatg tcgaaatagt agtggtagtg ccgtaactgc agtaccaaat
     1681 tctggctatc atttcgtcaa ctggagtgat agttctacat ctaacccaag aacagataca
     1741 aatgtaactg caaatatatc tgtaaccgca aattttgccc tgtcaaacga agacccgaca
     1801 gcaccgacat ctctcttaac agaaggctta accaatccaa ctggaattgg agatacaact
     1861 ccagagttta gtgcaatctt taatgatcca aacactggag atacaggtaa ctattaccaa
     1921 atacaggtta atacagcatc agattttaca ggaacctcta tgtgggatag ctctcttaca
     1981 gcaatgaccg ctactgcaat tggtgcaaga agcccagaca tctcctatgc tggtacaacc
     2041 ctcactttaa acggtacaac atactactgg agaattagat ttgcagacaa tcatgcagca
     2101 acaagtccat ggtcctctac agctaatttt aaaatgaact ctattccgac agcaccgaca
     2161 tctctcttaa cagaaggctt aaccaatcca actggaattg gagatacaac tccagagttt
     2221 agtgcaatct ttaatgatcc aaacactgga gatacaggta actattacca aatacaggtt
     2281 aatacagcat cagattttac aggaacctct atgtgggata gctctcttac agcaatgacc
     2341 gctactgcaa ttggtgcaag aagcccagac atctcctatg ctggtacaac cctcacttta
     2401 aacggtacaa catactactg gagaattaga tttgcagaca atcacaacga aacaggctca
     2461 tggtctagta caggaagttt tgtaatggat ggtgccccat ttgccccaac agcactatta
     2521 actgatggta tgacaaatcc aacaagaata ttctctacga caccgtcttt ctcagccata
     2581 tatacagatc caaatggaga cgatgcagat atatatcaaa tacaggtaaa tacaaataac
     2641 acatttacag gaactgtaat gtgggattcc ggtcaacagg caacatctac accagatgga
     2701 caaagaagcg aggaaattac ctatgcaggc acacctctaa caaatcaaaa cactacatac
     2761 tactggagaa ttaaattctg ggatttagaa gactatgagg gtccttggtc tgccacagcc
     2821 caattctctt cccttcaagc atccttcctc tttgaaggtg taaagatcgg tgaccttaaa
     2881 cttgattaat tgtagtataa tggccacatg agcttaaaaa tagaatcatt caaaaaatta
     2941 agaaaagaca aaagaaaatg gaaagtagac actccagaag agttcgagga aagagaagat
     3001 agcttcctca agaaacgctt aatgagtaaa gaaccaacca tagatacctt tgtaagaata
     3061 tcaggaggaa aggcaaaaaa ctttaaactt gaaattccaa aaacaacaag accactaaca
     3121 gacagaatga aaataaaaat cttcgacatc ttaagggaag acatcgttaa taaagacatt
     3181 ctggacctat atgcaggagc gggatctttc ggtctagaag cactctccag aggagcaaaa
     3241 tcagcaacat ttgtagatgc atctaaaaat gcatacttcg tactacagaa gaatatagcc
     3301 catactggat atcttccaga atcagaggta ataaaatcaa aaactgaaga ctacctcttc
     3361 aaacaaacaa accagaagaa aaagccaaag ttcgatattg tttttatgga tcctccatac
     3421 aaacttttca atacaaaaaa tatcataaaa atggaaagag ttatgaaaat ggcgtctcaa
     3481 ctaataatcg gaattcaaaa ccctaaaaca aatcgtttca aaggggcact aatcgttaaa
     3541 catccacgaa gatatccaat tgagaagata accatagatg gaatgaaaat catagaaact
     3601 tacgaaattg gtttaaactg tatatcaata tatatcgttg atttaaaaag ataattgcaa
     3661 aaacagtaag aaaaatataa aattagaggg atgtacaata aaacaggtct gcacaaagaa
     3721 aaaatctgta aaacagtttt tacaacacaa ctcttttttt aatgaggctt tttcccttaa
     3781 atataaactt ttagatttaa atatatgaat agtgatgttg aagaaatatc aaaaatagaa
     3841 aagattagac actctgcatc tcatgtactg gcacaagcag tccttaaact gtacccagat
     3901 gcaaaattag gcataggacc tgcaatagac aatggcttct actatgattt tgaattttca
     3961 gaacctattg aagaaacaga tttaccaaag atagaaaagg aaatgaagca gataatcaag
     4021 caggatttac caatcaagca gatttttatg aagcgaaaag aggctcagga atatctcgag
     4081 aagacagatc aaacatacaa attacagcta ctagaagaaa tcccagataa agaattaagt
     4141 ttctacataa ctggagtcaa cgatttcgca gatctttgtc gtggtcctca tgtagagagt
     4201 acaaaagaga ttggctttgt aaaacttaca aaaaccgcag gtgcttactg gaagggaagt
     4261 gagaaagaga agatgttaac cagaatatat ggtactgcat ttgaaacaaa agaagaatta
     4321 aaagatgctc ttgcaaaaat gcaactcgca gaacagagaa accatagaaa attaggaaaa
     4381 cagctagaac tctttgccat aatcccagaa attggacagg gattaccagt atggcttcca
     4441 agaggataca agattagaag aatacttgaa gagtatatgc ttgatcttga agaagaggct
     4501 ggatatcttc acatattaac accacatatt aacaaagaga tacttttcaa aacttctggc
     4561 catttagact tctacaagga ttctatgtat ccgccaatag ttatcgatga tgaaacttac
     4621 tatctaaaac caatgaactg tcccgcagga atgatggttt acaacctctc accaaaaagc
     4681 tacagagact taccgtacaa gctaggagag tttggaactg tgtacagata tgagaaaact
     4741 ggtgaactac acggactaca aagagttaga ggctttacac aaaatgatgc acatatcttc
     4801 tgcgcaccag accaacttga tgatgtactc aatgaaactt tagatctact agacatcttt
     4861 tacaaagatg tcggcttcga taattacaag ttcagactct cactttccga ctgggaaaac
     4921 aaaagtgata aatatgctgg agatccaaaa agatgggaat ttgcagaaca aactttaaga
     4981 caagtcctta acaacagagg attagaattt gaagagatgc caggagatgc tgctttctat
     5041 ggaccaaaga ttgatgtaca agccgttaat gtctttggaa aagaagactc aatttcaact
     5101 attcagttag atttcaatct ccctgagaaa tttgacatta aatatataga tgaagatgga
     5161 gtagagaaac agccatatat catccacaga gcactaattg gctcatttga aagattcttc
     5221 gccttcctca tagagcatca tggtggagat ttcccacttt ggcttgcacc tgaacaggtt
     5281 tacatcgttc caatttcaga gaaacacaac gactatgcca aacaggtcta cgcaagtatt
     5341 aaagaagcac atctaagagt aaaaatagat gacagaaaca agagtatgca atcaaaaatc
     5401 agggattcag agaagatgaa aatcccttac atcctaatac ttggagacaa ggagttggat
     5461 actcagactg tttcaattag agcaagaaag agtaatgagc atggactaat gaagactcaa
     5521 gaattcatag acacattgaa agaagaggtc cgcaccaaag gtaacccttc agagaattaa
     5581 atttttaata taaataatta atgtcttacg gagagaaaag agagtttaag aaagattacg
     5641 gtccacgagt aaatgaatat attcgtgtac cacaggtgca attaattgac gaaaagggag
     5701 agaatttagg tgttgtagat acagaacaag caagaaaact cgctttagaa gcagaactag
     5761 atctagtaga ggtaggatca agtgctaaac ctccagtttg cagaattatg aacttttcga
     5821 aatatgtcta cgaacaaaac aagaagaaaa gacagaataa aggcggaaaa atgaaagaac
     5881 agaaggaatt cagatttaca ccagtcatgg atatcggaga tacagattac agaataaaga
     5941 gggctcatga atttcttaca aaaggacacc aagtaaaact cacaatggtt aaaaaaggca
     6001 gacagagtat ggatcaagca aggtctgttt ttactgaaat attgactaat tttgcggatt
     6061 atagtagtat cgaagccgaa ccaaagagtg aaggaaacag aatttcaatt acttttaaat
     6121 cagatggcaa gacaaaaaac aaacaaaaca gcgaagaaaa gaatcaggct atccaacccg
     6181 agggggaaca gaaagccaaa attaagatat aagcaaagtc accagggtca tttgaagaca
     6241 aaaagatctt caagatctaa gagaagacag ctttctaatg ccattgttgg taaaactaat
     6301 gatagaaaaa ttagaagaaa aatagttaac atctagatat acaaacatga gagtaaaagg
     6361 tggaaaagta agaagtagaa aacataataa agtcctagca tcaacaaaag gatatagaat
     6421 gacaaagagc aacctttaca aggttgcaca cgaggcatat cttcacgcag ggcagtatgc
     6481 atacaatcac agacagagaa aagcaagtga gatgagaaga gtatggattt ccagaataaa
     6541 tgcagcttgc aagaataata atattcaata taaagattta atcgatggtc ttaagaaaaa
     6601 agggagtaca ttaaatagaa tggtactagc agacattgca tacaataatc cagaggtatt
     6661 ttcctttata gtaaaatctc tgtagagtac ctaaatttta gatatttaaa ggactctaca
     6721 aggggtcctt tttcttttta agataaaaat atacaatata agaagcatgc atacattaaa
     6781 gactttagaa aagataaaaa ccgagatatt ggaaagcaac agtagttact ctgaattcaa
     6841 agaaaaatat ctggggacaa aaggacttgt tacacttgca ctaaaagata tcaccaccat
     6901 agacaaaaag gatagggcag agtatgggaa acagataaat gaaataaaaa acatttgtaa
     6961 cgatattctc caaaagaaaa aattacaaga agagaaaata catacaagca aaaagattga
     7021 cccaacagca ccctttgaca taaatacacc ttcagacaaa ttgcctgaga ttttagattt
     7081 aaaaggttct aaacatccac tacataaaga agtagaaata tttacagata tatttaaaga
     7141 tatgggtttt gacatactgg aaagcagaca aattgacgat gactaccata tgtttgaatc
     7201 cctcaatttt cctaaaggac atcctgcaag agacatgtat gacaccttct ggacagagga
     7261 cggttttgta ctaccagcac acacatctac aatgcaaaac agagcattaa agaaattcgg
     7321 ccctccacca attagagtag tacttcctgg gagatgcttt agaaacgaag caacagatgc
     7381 tagtcatgaa catacttttt accaaataga gggcatttat gttgacaaag gaatttcact
     7441 tgcaaatatg caagcaacta taaaaacatt tctagaagcg tatctcgaaa aagaattaga
     7501 agtaaaaata caaccagctt tctttccatt cacagaacca gattgtgaat ttataattag
     7561 ctgtcctttc tgtgacaaag taggatgttc aacatgtaat cactctggtt ggatagagtt
     7621 aatgggatgt ggaatgattc atccaaatgt tctcaagcaa ggagggatca acccagacat
     7681 atattctggc tttgcatggg gatttggtgt agacagatta acaatgatta aacttggaat
     7741 aaaagacatc cgtcttctta gagactctaa cctaaacttc cttagacagt tttaaatatg
     7801 aaagcaccta ttaatcttct gaaaaaacta agcggtgtga aattagatga caaggaaatt
     7861 gtcagattaa tatccgagca tattggacag gtggaggatt tccatgatct aagaggagat
     7921 tacaaaggga tactcgtagc agagataaag gagaaacagg atcatccaaa tgcagataaa
     7981 ttagccatat acaaaatctc aacaggagac aagaagttaa tacaagttgt agctggagac
     8041 aagactctcg aagtaggcga taaagtcgca tatctaagac ctggaagcaa gatcccttac
     8101 acaatatata cagaggccga accagtaata atctcaaaaa gaaaaatacg gggtgttgaa
     8161 tccaatggta tgttaggtgc agaaaaagaa ttaaacctcg gatcagatta caccaaggtt
     8221 atgagacttc ctaaagacgc accaattgga gaagactttg ctaaatatta cgaactagat
     8281 gacacaatcg tagatataga gaacaaggct cttacaaata gaggcgacct atttgggata
     8341 ctcggaatag caagagaatt atctgcaatt acaaataaca aatttgaatc cccacagtgg
     8401 tatttagata atgataaaaa atataccgaa gagacaagct gtttaaatat agaaattaaa
     8461 aacgatgctg aagtaatatg tccgagatat acagccatat catttgacaa tattaacatc
     8521 caggaatctc tcatatggct caaatctttc ttagttaaat ctggaattgc atctatcaac
     8581 aatgtcgttg atatcacaaa ttatgtagct caccttgttg gtcaacctct tcatgccttt
     8641 gattacgaca aattagtaaa caacgatttg aaagcaaaag gaactgcaag aataaatatt
     8701 aggatggcaa aaattggaga gacaatactc gctctagata ataagatcta tgaactagat
     8761 gagaatataa tggtaatcgc agatagtacc cacccaatag ccatcgcagg cattatagga
     8821 ggaaaagata ctgaggtaga tgcgaatacc aaaaggattg tacttgagtg tgctaacttc
     8881 gacaaaacag caattagaaa aacatccatg aggcttggaa tttcaacaga agctgccaca
     8941 aaatttaaac atgcaataag cccagataca tgtattccta cattaaacaa agcaattgaa
     9001 ctaattagag aactgacaga ggcctcgata gcatcagaca tagtggacat atattcacta
     9061 ccacaaagaa ccaagtcact ttctttttct ctagcttctc taaatacaca tcttggaaca
     9121 gacttggata aagaaactgt cgaaaagata ctctcaaata tggaatatgg aatggagaaa
     9181 gaagatgaag ggtatgtgaa ggtatctatt ccttactggc gtatggatat agatattaaa
     9241 gaagatattt ttgaggatgt tggaaggata tatggataca acaatataaa attagaatta
     9301 cccactcgaa agataaagcc atctgggatt aataaacttt acaatttaaa aagacaaatt
     9361 cgagagattc tttcgaatag tggtgcaaat gagaccgata catatagttt tacagatgtt
     9421 caaacattga agaaatgtaa tcaagatcca gagtttgcat ttagaataaa aaatgcacta
     9481 gccccagagc tttcattaat gcgaacatca ttaatatcct cccttctaac aaagacagaa
     9541 gaaaatattc agagagggtt caatcaattt gcactctttg aaatgaatat tgcacatcaa
     9601 agacagctcg tggatggaaa tggcttacca attgaatcat ggtctcttgc tatggtcatc
     9661 tgtgacgcta caaacaattc tcagacctct gcttactacc ttgctaaaag atacattgcg
     9721 aagatattct ccacgttaca tatcgaaggg attggatact cattgatatc cgagagtagt
     9781 gaaaaagaac taccaataaa tattaaaaac ttaatctatt cctttgaatc aaacagaact
     9841 gcattgataa agtacaacga tatcgtcctc ggtgtaatag gggaattcaa ctctgtggta
     9901 gtaaataatt tccaattacc cgcatactct tctggcttgg agattgatct aaatgctttg
     9961 cttaatgtag aacagaaaag taaacagtac agagagatgc ccaaatatcc agcaagcata
    10021 aatgatgtct gtattgaggt aaacaaagat actcaatata tcgatttgga aaacgagata
    10081 aaaaagattg tcaacaagga taaactttgg ggagaggttt cctgtattga tatatactcc
    10141 aaggggaaag aagattcgaa aaaacgaatt actttccgat taaccttaag acataatagt
    10201 aaaacattta atagcaacga tatctcttca ataatatgca acattgaaaa gaatatccga
    10261 gagaaattcg atgctactgt tatatagtat tcctatggaa ttataattgg ttctggttct
    10321 tgcttaacaa cattaatagt tacactcttt gtactacttt tacccttatt atctactaca
    10381 gttatactca cggtgtgatc ccctagtgga gtagaaatat ctacaaagta atcaattaca
    10441 aatgtaccat tcggattata tgtagcacca agaattgcat taccgtcaaa cttagcttca
    10501 aaggtcgcta tactctccat gacagacaca gaacctgtaa tttctaagtt attacctttg
    10561 acaatctttt gtccacttat tggttttgtt aattctatca aaggtgcatt gtcaggacct
    10621 aaaggtagag gacatatgcc tgtcgcagga gctttgggta gataatttga attcttcata
    10681 cttaaaagat actttgtata tgcatctctt tggtactgat tttccaaagt tgttgaaagt
    10741 acaatctttg tagttgtaag tccatatttc cttgctgctt caagattctc tggaattaag
    10801 ccattagcct tacatatctc atatgctttt ctgttgtcta cctgtggagc tcttccagag
    10861 atatatacag atgcctcctt cttacaatca accccatctg ctggagttgc accagtatct
    10921 gtacaaacag ttgtagagag tactcctgct ggtcgattat atgttgttgg cttgtattta
    10981 ccagctactc ttgtcatgaa cgaatttgca atgcctaaag gtactgtaga accccatgca
    11041 cctggtaaaa cttgattgtt attatttcca ttccatacac caactatcaa atttggatga
    11101 tacaggattg tagttaaatc ctttggacca tccgttgttc ctgtttttcc acaaactctt
    11161 gttttattta ccgtatattt tgaattattc ggtctatcat tcatccctcc caaatcacaa
    11221 agcatccaat ttagaagata tatctctttc tcatcaaata ctcgttgacc tttattttct
    11281 cctgcttcct gtaagacttc tcccttagag tcttcaactt ttagtataga aatgacatct
    11341 ttttttattc cgccagttgc aaataccata taaccagctg tatgctcaag aagtttttct
    11401 tcccctgaac caagagcaag acttaatccg tatgactcct tatttgagaa attagtaaca
    11461 ccaactttct gcatagtttg gaggaaagca tctattccaa ctaattgtaa attgtaaaca
    11521 gcagaaacat ttcgagattc tacaatggct tttctagatg ttatgagacc catatattta
    11581 ctatctgaat tttttggatc atatgttcca aattttatct cactcaaatc tggagcaacc
    11641 atccatgggc catagccctt ggttatagca gttagatata cgaaaggctt caccgatgag
    11701 cccattggtc tattacttaa agctatattt acatttccat caactctagg atcttttaca
    11761 ttccagtaat ctacagaacc taccattgcg agaagctcac ctgtcttagg atctattact
    11821 accattgaac cgttgtgtac attgtatctc tttgctccat ccactccctt tgcaatttct
    11881 tcttctgcta tctgttgaat tgaagaatca agagaagttg taactttcag tcctcctctc
    11941 tctacacgat ccactccaaa ttcttcttct aacaaactct taacatagaa aacaaaatgg
    12001 ggtgccttaa tgtcaatcct tttagtattg tataccaact cctgatcctt tgctgcctgt
    12061 atttcttcct ctgttacacc tgttaagttc ttgtgcttca acatttgatc aagaacatat
    12121 gtctgcctaa cctttgctaa atctggatta gtaccataca gaggagaata tacaccagga
    12181 ctctgtatta atcctgcaat taatgcactt tctgctaaat ctaaatcctc tacatttttc
    12241 ccaaagtatg cgtttgcagc ggcttcaaaa ccataattaa caccaccaag agggatttcg
    12301 ttcatataca tttgcaagat ttcatcctta gagaaagtct gttcaacttg catagtaatc
    12361 aaaatctctt ttatctttct tgaatatgtt tgcttatatg cctcatctcc aagaacatca
    12421 tacaaaattg ttgttttaac taactgttgt gtaattgtgc ttgcacctct aacaacacta
    12481 cccgccgtaa agttttgtat tacagccttt gctataccca tgtaatctac acccttgtgc
    12541 tgatagaatt ctatatcctc agctgctaaa agtgcccatt tggtatgctc ttggatcttg
    12601 tctatagata caaattctct attttgatct ccatatacgg tataaagtaa ttttccattt
    12661 ctatcaaaaa tctgtgtact ctgatccgaa gtcctctcaa ccaattcgtc tggagaaggt
    12721 agagatttct gaagattttt aagatacaaa cctaatccaa gaagtaccag acaaccaaga
    12781 aagaatccta tacccaccaa gatatataga gctttcttta gttttttctt aaatagcaat
    12841 ttctttccag acaaatctgt acttttctct ccctttactt tctttctatt ggaaagattg
    12901 tattttttga aattattgta ccttgcaaaa tccatcctcc tcaaagaatt tgatccaact
    12961 gattttcccg ggctattcgt tacactaacg ctatatctca ttgaaatttt gcattaatta
    13021 atacctttaa tatatccgaa ttaccattaa ctttcaagac taataggaat gctttgtaat
    13081 ccttttgtaa gaatttttca attaaaatta tattatatga aaaatcttcg acagacacgg
    13141 tttcttttga tttctaattc ttcactattt aaacaattaa taagagataa cctacatttg
    13201 caggggagta ctctaagttg taatacagta atttcggcaa aagggtctct tgatactgaa
    13261 ttgttctctg ctattattat agatcaagat atacaatctc aagatagtac agcttacagt
    13321 ctgcttctat tttcaattta tcaaaaagat ctctcagatt ctgtaattat tttctccaaa
    13381 gagatttcct cggagactat ttacatgtat tacaaataca ggatacaaaa catattcttt
    13441 ctttcatcag ttaatctttt cctagaacct gtaattagaa gaattctttc tttaatacca
    13501 gaaacagatg gcatggttct aagagatagg gggatttcgc tttacttaag taataattat
    13561 gcaatattta aaggttgtag aatattactc tccaagactg agaaattaat tctcgcttat
    13621 ttgcttaaga aatgctccct ttgtactaaa acagaattga ttaattattt gtccaacaca
    13681 ttgcaaagag aaatttcaga aggatattta acagtgaaca tctcgagatt aagaaaaaag
    13741 tttgtcaaac atactgggat taatatcgta aagagtagga atgggtttgg atattatcta
    13801 tctgtctaga ttttcttttt ccaaccaacg attttgttta atgcctcaat tggggttaaa
    13861 ttgtctaaat ccaaagattg tatctctttt tcaatctcag aatcttttgc ggtaaaaaga
    13921 ggatactgaa gaccagagtt accatttctt ggaatattct tagacgaatt aagtacatta
    13981 atatctctta attcattctt cttcacaaac atactctctt gttcaaaact ctcaagtatt
    14041 tcatttgccc ttttgataat tttgtttggt aatcctgcca tttttgcgac atatattccg
    14101 taacttctgt ctgtaccacc agctattatt tttctaagaa atattactgt accttcctcg
    14161 agattttctt ccaccatcac gttgtaattc ttgacagcat ctggatattt ttcttctaac
    14221 ttaagtaact cgtgatagtg tgtagcaaaa agagttctag cttgagtttc agatattaga
    14281 tactccgcta gagcccaggc tatacttaca ccatcatatg tactggtacc tcttccaatc
    14341 tcatctaaaa caattaagct gtactttgta gcattgttaa caatattcgc agcttcatcc
    14401 atttctacca tgaaggtact ccttcctctt gccaaatcat ctgaagcacc gacccttgta
    14461 aatattctgt ctaccaaaga gatacttgct tctttagctg gaacaaaact tccaatgtgt
    14521 gccattagaa caataacggc aacctgccta atatatgtgg actttcctga catatttggt
    14581 ccagtaagta tacacatacg gttatttttt gaatctagtt gtgtatcatt cgaaatgaac
    14641 tcactttctg taatattttc aatgattgga tgtctccctt tttcaatatt aataacaccg
    14701 tctttctcgc ccatctcata tatctctggt ctacagtagt tatttttgtg tgcaatatgt
    14761 gcgaaattgg aaagaacatc aatgttagat aggtattgag aacatttctg taattcacct
    14821 aatacaggaa agaatgaatc cctgaactca caaaatagtt gatattctaa tgaacatact
    14881 ttctcttgtg cattaagaat aatgttttcc ttttccttaa gttcttcagt aatatatctt
    14941 tctgcgttaa caagtgtctg tttcctgata tatctttctg ggactttctc tttgtgtgca
    15001 ttggttacct ctatgtaata tccaaatact ttgttaaaac taattttcaa agaagatatt
    15061 cctgtatctt ttttctcttt cttctcgaac tcttccaacc aagttttgct atctcctgag
    15121 agagatctaa gttcatctac ttcttggtta aatcctgttt ttagtatatc cccatccaag
    15181 agagagagtg gaggagagtc tgagattgtt ttttcaattt tggagattaa tccttcaatt
    15241 aattttttat caatatcaaa agatatatca aattcctttt ttaagatatc tgctatctcc
    15301 aagaaattct taagcgatgt ttgtaaataa ttgaaatctc tagcattgac tcttccgagt
    15361 ccaatctttc ctacaattct ttctacatct ccaatttcac ttaacctttc ccttatcttt
    15421 tctagttcgc ttttcttagt aaacaatttt tctactttct ccaatctttc ttctatcctt
    15481 ttcttgtcta acagaggatt taaaatccat gaaaagagaa gtcttttacc cattggagta
    15541 caggtattat ctagaacaga gagaagagaa ttagatatgt ctccagagta tgcatttcta
    15601 attaattcta gatttcggat agttgcacta tccaagacca ttgtgttatc tatactcact
    15661 tttctaggct tctgaatatg cttcggtgac attaattgag tttctttgat atattccagt
    15721 atcattgcaa cacttacaat atctaaggat tctccttcaa taccaagact ttcaatactc
    15781 tttaaatcaa agaaatcttt acatacctca gaggagtatt gtttgttttt taaaccttta
    15841 gatacaaagt gaactggttt gtcatcaaat ctgaaacttt cctctccctc aacgaggagt
    15901 atttcttctg gatcatagga ggtcagtatt ctgtttgcga tatctttctt gttactttca
    15961 gatattagga gaaactcacc tgtggagaca tcacacaagg caacagaggt tagtgtcttt
    16021 tctttagaga atgctgctag atactggttt ttagaacttt ttgctttctc accatctagt
    16081 gttccagggg ttactattct tgtaatacct ctttttacaa ttccttttgc gaatttgggg
    16141 tcttccaatt gatctacaac tactacacag taaccacctt caattagttt aggaagatat
    16201 tggtctatgg ctttgtgtgg gaaaccagca agtggagtag ggttgttttt tttgtctctg
    16261 gtagtaaggg taatgtttag tactttggag gctatttttg catcttctcc gaatgtttca
    16321 aagaaatctc ccattctgta aagcagtatt ttatctgggt attgcttttt aaagccctga
    16381 tattgtctca tcataggtgt gttttccatg tgtgaaaaag tatatcatga aaagggatat
    16441 ttagactaca ggttactttc cgaggattaa ttttgcttct tcttcagaaa ctacctttct
    16501 tgcttcaaca atatcttctg gtaatacaca tacaccatat actccagatt ggacacagaa
    16561 tcttagtaga ggtttgctat cttcaactat ggctatgatt ttcgtttctt tgtttttaac
    16621 aacttctaat atattctcca ctatcttaaa cacactgttt cttcccaaat catacttagc
    16681 attctttgca tcaagtttaa taccctgcat ctgttttgca attcttggca tatttagaat
    16741 taaaccatct atcttggtat tcactatctc atctgcaaga ataacctcag atggattatc
    16801 tagtatggca tagatactaa aggaagaact tcttcttagt ttctctccaa ggagaatctt
    16861 ttttaactct ttcataatat cttcattcat tggagcatgg atacccaaag aaacatttct
    16921 ttttttgtaa acatttctaa tccttttaac tatcttcaaa acccgtctaa acatttccaa
    16981 attatttata taatgtgtac caccaaaaac ttcatcagaa agaacactgt tttcaaattc
    17041 tttacctcct acaagagcct taaacttctc aacagtacca gaaccaattg aaagaacaac
    17101 ctcatctccc tgtgccaaat ctacatactc cattaccttc tcacatatct tggtagagta
    17161 atctacaaat tttttctgct ctacatatga gagaatatgt cgtccatctt gtagcataat
    17221 ctcgtctaaa tccacataga caataccatt agaattaccg acagtattga gcatgtcctt
    17281 ggcaggtatg gtacacatag agaaaacttt tgtagcagaa ggagagagtg tactatctct
    17341 tggaattaca aggcctggtt tctcttcttc aacttttacc tcttctttct tttcaagaag
    17401 atctacactt acgacattac catctccata tgcctctaca acagggtgta gtgcctcttc
    17461 cttctcttca acctgtctat atatacttcc agcattacca tctaacttga ccatgtctcc
    17521 atccttaaga atctcagatg ctccctgagc tccaacaata gctggaatct tgcactctct
    17581 acaaagaatg gcagtatcac ttgttaaacc tccagtttcc ataatgacac caccacagga
    17641 gattatcatg gattccattt ccgaggaata cttttttact agtaatatat ctttgttgtt
    17701 aaattgcttg ttcacaaacc cttcaactat ggacaccttt cctgtggcta caccaaagct
    17761 agcacctata cctgagagta caaaatcttc aatatttgtc ttgttttctc tcgcttcttc
    17821 tgttttctgc tttgcaattc caactagttt ctcaaatttt tctccgactt cattgctact
    17881 tctttgaact attctctgag cttcggacat cgcttgagac acaagcatct cctctccacg
    17941 atacctctcc aaaagtgcaa ctatgacctc tcgaaggttt cttttggcca tattcatatc
    18001 tccaacaaca atcctgtctc tatgctctcc aaataatggt ttgttctgta aaacccaaac
    18061 ctttccacca gaaataaccc attcaacatt ttggacttct ctagatttgt tctcgattat
    18121 tagagcaatc ttagatatct cttgcatatc tttgtcattt aatttctgtc tgtgactcca
    18181 actatttgag atcttaatct tttcctcatt gcctcgtcga ccccttccct gattcatagt
    18241 cctaatcttc atccactctt gaggagcaat atgtttttct acaatactta aatctcgttt
    18301 gttcagggta tatgaatctg gagtaatctc tcctaaagag attacatctc caagaccata
    18361 taccgcttca atacttaatt tgccctcatc ttgtgttatt gggtcaattg taaataccac
    18421 tccagatatc tctgactgaa ccatcttttg gattacaatt gctagtttta cctccgagag
    18481 atccacatcc attttagaag catatgcaac aacatcgtca gtaaacatgg aggcatatat
    18541 cctttttact gcacttaaaa ggtctgaaaa atgtctaacg tttaattcgg tagaaaatat
    18601 tccactgaaa gatacctctt tgttctcagg gaacactaca gaggatctta cagataccca
    18661 agcatcagaa aagccagaaa gtcttgtata tgcactcagg atttcttctt ctatctcttt
    18721 acctaaatca tttttcaaga atacatctgc aacttcctct ccctcaggat ttctaccatt
    18781 ttcgagtagt tttttttctt cagaagctag tgccttaaca gagaaatcta aaaatacctt
    18841 acttgaaatt acaaaaaaat ctggaactgg aacatccata tccttgagct ggaagagact
    18901 tagacccttt ctaccaactt gattttcatt taaatccttt gacgagaaat cttcgaaaaa
    18961 gaatacattg ctcttttttg catcactatt gtccattcgt atacttggca gttaaataat
    19021 ggttatatac tactaaacga tacctttagc ttttaatttt tgcaagtcta tgtaaaattc
    19081 ataagcaatc tttgctaaaa ccattactgg cacagcaata agagcaccaa agattccaaa
    19141 aaggagtagg gcacttacaa caccaagcat aacgataact ggtttaatac caacagcatt
    19201 acccattatt tttggcacaa tgaaattatt ctctatctgt tgatatatta ttgaagctat
    19261 aactacaata attgttccaa ctggcccaac cgtaattaag gaaacaataa tagctggaac
    19321 aagtccaatt gttgctccga tatttggtat tgcatcaaga agggctatga ataccgcaag
    19381 tggtaaggca aaaggaactt taacaattgt caaaattatt aatgcgacaa ttcctgcgat
    19441 tgtacttact gtaagctgtc caagaagcca tcttcccaat tttttctgta catccacaaa
    19501 taatttctta actctatttc tcttttcatc tgctgcaatt ctaattaatc caaactccaa
    19561 gagagaatcg tattccaaca ccatgtaaat agaaaatatt aaagatgtaa ttactaaagt
    19621 tgtggtgcta aagaacgagg agagtgcatc tgttatgttt ttgaatccat ctgctgtagg
    19681 aagattttta aaaaaatctg ttacatacgt agatacagta cttgcatcaa ttgagaaacc
    19741 tgcaaaggaa atgcctttaa aatttgcaat ctcctgagat atccatgtag gaagaactgc
    19801 aacaaattcc gccaattggc ttacaaaagg aataataaca attgagaaca aaccgagaaa
    19861 gactaaaatc ccaagaagat atgtgattaa tacagcccaa cctttagata tttttttgct
    19921 tgttagccaa tctacaacgg gttttgcagc tgtcataaag acaaatccta agaatacaaa
    19981 aatggcaaca gttgaaagct gcttacccca aaagattacc gctaacagta gtacaaaaaa
    20041 tattactatt ttccaagaaa attctacaac aaccaaaggc cttctatctc cttcttttcg
    20101 ctgatactcc aaaacatctg caagttcctt tctatctctt gtttcttctt ttaattcttt
    20161 actattatcc ccaaaagaaa atatcttttt catttaatgt atagaaaaat ttactgatat
    20221 gattatacca actatttacc tgcatatact agagaaaaag agggagttta aaaactcccc
    20281 ttttaaagac taagcttttc tgtttttata cagtaaaata cctgtactag agaacattcc
    20341 aatcaaggcc aatattccaa atataatcat caatttgtta cccaggagag tagacattgc
    20401 atcactcaat tcaccagcag cgactggaat agagctatca tcaactgctt ctccatttac
    20461 atcactatct tccgagtata ttacccgtat cttatcttca acttttgttt catttcccgc
    20521 ttcatcctta agttctatat taaaaacatt tggccccttc tcaaggacta ttccagatat
    20581 cttaaaatta cccttttcat cacaagttgc tctgtaaagc ccagttcctc tctttacagt
    20641 aactactgca tttggttcta caactccagt caaagagaat gtctcagttc caacctctgc
    20701 gggatattta atttttgaaa ttgtaggagc tgttttatct accgttacat ttagaacctc
    20761 ggaaattgga gaaatatatc ttcttggaaa cccttttact ccagccactg ttacagcata
    20821 tttaccttca tccacaagat caacggtata ctcatatgaa ccgtctttag taactttagt
    20881 tcttgcaact tcctcaccat ctacataaat gaatacttga tcatacccac ttgcagaacc
    20941 acgaattact acagaacctt ggtttgtaag tgtatttact ccaactatta caggagcctc
    21001 atatgtggta ttaatctctg gatttctatt gtatttaata aatgaatata ttccgaaaga
    21061 tattaaaagg acccccagta caataaaaat tgtccctact attcgggaga ctctttcttg
    21121 atcttgaggt ttctttatct caattttaga gaccatagat tccttctttt ggggaatatt
    21181 tttggacgca gtttgtatac tagatttaga ttttttcttt gccacgctaa acctatatta
    21241 aaatttatat accttcttat tctagaatag tataatacat aaattaatgc aactatcttc
    21301 gatgatatga acaaaggatc aaaaataata ataacagtgc tccttctact attagcacta
    21361 gtgccccttg tatacttctt agtataccca gtctacaacg acatcctcat gaagaagaat
    21421 tctctggaca tgtaccgctt ttctaaacag atctccataa atcttctaac tcaaatatct
    21481 cctagcgcag atgtgttttc caaagaaata cttggagtag atagcatgga agaatctctt
    21541 aagtcggaaa atcttgttct caaagatacc ttactggagg acatgaaaac aaaagtcata
    21601 atagattctg caaatataga gggatatatt gcacaaggtt taagatctga aaccatgatg
    21661 cgaggcttct ggcattttcc aacttcagtg tatcccggag aacttggaaa ctctgtaata
    21721 attgcacatc gcttccagta tcttcctcct gccaaaaata cttttttcaa cctagataaa
    21781 gtaagagtag gagataatgt cattttacaa ggagagagtg gcgagtacac atacatagtt
    21841 acaaatatta aagaggtaga tccaaatgac acatccgttt taatgcaaac tgacgactac
    21901 agagttacac taattacatg cacaccactt tggactaatc acaaacgctt ggtagtaaca
    21961 gcaaaactag acaaactata caaaaaagtg tagacttttc aaacaaattt actaatttaa
    22021 accctaaata catggaacag ttttacgaaa gaaaagaaat ttctaaagat acagttgaat
    22081 tgaaaattac gattcctaag gatagcttta aagatagtta caacatttta cttaaagacc
    22141 atcttaaaga tacaagtgtt aaaggcttta gaaagggaaa agtcccaaca gatttactag
    22201 agagtgacct taaaggggaa ttaaaaatcg agaccttaga caaactagct ccactttaca
    22261 ttaataccgc aattcaaaaa gaaaatattg aaccaattgc tcctccaaca tacaaagagc
    22321 ttccaaagtt agaagatgac aaggatgttg tgtttacaat tgagattaca gtacttcctg
    22381 agtttaagtt aggtaatatg aaaaagatta agatagagaa agaggaagta aagataacaa
    22441 aagctgagat tgacactgca ttagaggaga taaaaaagaa cagggaaaca aaaacaaaag
    22501 aggttaatga caaatgggca gttgagattg cagaaatgtt aaagattgaa aaggttaaag
    22561 atttggaatc acttaaaaaa tatataaaag aagctcttaa gaagcaaaaa gagcatatgt
    22621 ttctgcacaa gagagaagag gaagcacttg tacaggcaat atctctttca aagattgaga
    22681 taccacttcc agctgtaaag tttgaagcaa gtgagagaga aagatctttc atctacgata
    22741 tgcaacagag aggtgtaaaa ccagaagaat tcatgaaagc acaaaatatt accatagaga
    22801 aaatgagaga gatgtgggaa aaggatgcaa cagaagcttt ggaaacagat gtgtttttga
    22861 aagtttataa aaaagagaac aagatttctg tatccgagat agatttgaat gaaagaatag
    22921 atgaaattaa aaagtctgct ccaaagggca gagacacaag tgtatacgat gatccacaat
    22981 ggagagagta cgttcaatct gtatatgaga aagagaaagc ttttgaccat tttaggaaag
    23041 aggttttagg agatatacat aaagattaat ttaatctttt tacattatgt atttaatacc
    23101 aacagtaatt gaaaaagata gggatggaga aagagcctac gatatttact ccagacttct
    23161 taaggataga attttgtttg taacgggtcc aattgaaaca gagatggcaa atactgtagt
    23221 agcacaactc cttttccttg agaaagagaa tcccaaggaa gatatccaga tgttcataaa
    23281 ctcgcagggt ggtcagatta cagcaggtat ggcaatttta gatacaatga attacatcaa
    23341 gtgtgagata tctacaatag cagtaggtct tgcagcgtcc atgggtacag tacttttagc
    23401 aaatggtaca aagggtaaga ggtttgcgtt accaaatagc aagatacata ttcaccaacc
    23461 aattggtggg gtagagggtc aggctagtga tatagccata gaggctgagg agatattgaa
    23521 aacgaagaaa cttttgtatg aatttctttc agaaaaaact ggtaagagta tgaaacagat
    23581 agagaaggat gcagatagag ataagtattt cacagctaag gaagcaaagg agtatggatt
    23641 aatagataaa gttattaaaa atagataatg gcattagatg atttaacagg agtagaggat
    23701 caaaggaaac aagatccact tgattccatc ctttttaaca ttaatcaaat gtatgagtat
    23761 ggtacagatg cagataccgt ttctttagac ttagataatg tatcacttca agatgttgat
    23821 agatatttaa aaaaagtaga gagagatcaa gataccccta taaaattgtt taccgttaat
    23881 ttcgcaggcc tttattctcc aaacgaggct ccccaaatta tcgatgaggc tttcgtcaat
    23941 ctagctgagt acccaaatat ccaaaatgtg tattttttag agggagggaa attcgcatta
    24001 gcattattcc caatcatcgt tgagaataaa aagctttgtg ttattataca gcccttagag
    24061 caatctcttc cattcgtaaa aagagagtat tacaaagtag tagaacagag acataacaaa
    24121 agaaaaacag ttgaagagtt aaaagaacaa atctcttttg taaaattccc taaacagaag
    24181 taactaaatg ttcttgtaca aacttcttaa tcctttctac aaccttatcc atatctttca
    24241 ccttactaga tgctgtgtat acagtaatat cgaataactc atcattagta gcgtctaacc
    24301 agtatccatt cttgtacagg taaagtagta gggagaatat tcccattctc ttgtttccat
    24361 cttcaaatgg gtggttctta atacagaagt aatacagcat acttccctta tctactagag
    24421 tagggtacaa ctccttcccc tctatacttt ggaatggttg agcaagggag ctttctaatc
    24481 ttccttcatc tttaacaata tatcctggat cattgttaat ctctttaatc atgtagtctg
    24541 agaatagttg tgcaatatat tttaattctt ctttggttat cattaggagt tttgtaatct
    24601 aagtattgct tgtttgtatt gtttcattgt tttagtaaat acttttttaa ggagtatttg
    24661 ttgttcttta gttagttggt tattcatatt tgtattatac ataatacaaa gaaagatggt
    24721 aaaatatata ttaaagaggg cgattagctc agctggttag agcatcacat tgacattgtg
    24781 gaggtcacag gttcgagtcc cgtatcgccc accatccatc tttttctgcc tgtaacttag
    24841 attccgggga aagtgagaaa agcaagtctc caagcatctc agatggtatt ttcaacacta
    24901 ttttaagttt gtatgggatc atctgtcctt ctttcttagg accagatatg ctatctgatt
    24961 ttctctttgg tctagagaag acaataattt catggatcat catatggatt aaaccatata
    25021 gataatcaat attcttgtta tcaagataat ctttcatagc ctcctcatac ttctcttgga
    25081 atagctcaaa tgctttaaag tattcctctg tatttgagaa tattgataat tctgattcaa
    25141 ggatttcctt ctcctttata ttccttccat attgagcatc caattcatca atctctttga
    25201 tctttctttc tctagtgtag tctccatctc tatacataat ctcaatcctt ctttttgcac
    25261 tctcagtgct atttaataac tgttttacaa tttttaatct atctttcttg gctctaacat
    25321 cagccttttt agactggaga ctttgttgat aattgaatac aacctttgga ttattcagta
    25381 gttctctgac aaattctatt acatgctttt ctaaaggcag agctggtagg ggaatagttt
    25441 tacaatctat atttcttctt tcttttttat tactattctt tgaactacat tggtagtaat
    25501 agactttctc acctgtagat ttaactttct ttggaaaacc attccaagca taaggttctg
    25561 tatgaatagt agggttgtaa cagtttgcac actttaggag accttgtagc agatacaatc
    25621 tactttcttt ttgaactctc ttatatctag ttgtactagc tgatatatat tcttgagctt
    25681 tcttaaatag ttctttatct ataaagtctt ggtcatggta atagactccc catttatctt
    25741 ttggtacttt ctttttatct actgttttat tgtaatactg tataccaata tacaactcat
    25801 ttgttagaat atttcttaca gcacttggat cccaagcata tggtcctcca gcacttaact
    25861 tccttccctt ttgttcctct ttattatgtt tatatctata tgcttgtgga gtcattatct
    25921 tatgttcttt caagtatctt gctatatcat ttacattcct tccttctctg acaaacatat
    25981 taaatattaa ctcaacaact tttctttctt cctcttggat tagaatcttt ccatcattac
    26041 ccttgatata tcctaatgga ggagtattca tatatgtacc ttgttctgca gatgcttttc
    26101 taccactgga tgttctctcc ttgatatttt ccatttctaa ttcagcaatc acacctatga
    26161 ttccaagcat tgctctccca aatggagtag aggtatctat actttcatta gcagagagaa
    26221 attctatttt ctcctcacct ttatcaaaca tatctaatat ctctaaaaga atcttcaatc
    26281 ttcttgcaaa cctgtctatc ttgtatacag caaccacatc aaaaggtctt ttggtagatc
    26341 tagagatatc ctctaacaat cttctaaatt caggtctgtc cattggctct gtagtaccac
    26401 taacaccctc atctatgtaa acatactgtt cccctgcaaa ctctagattg tttcccttag
    26461 aggctattaa acccattaga gagcttcttt gaaggtcaac accgtacatt tctacctgtt
    26521 cttctgtaga aacccttata tacaatgcta cttttttctt tactttttct tcttccatta
    26581 aataaggaga cacaacatgg aacttaaata ttacttctta tctcttgcct tggtatata
//
LOCUS       DAOQWP010000010        25974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_2189_length_25974_cov_7.358540, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000010 DAOQWP010000000
VERSION     DAOQWP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 25974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25974
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2189_length_25974_cov_7.358540"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..206
                     /locus_tag="PKI16_01900"
     tRNA            124..206
                     /locus_tag="PKI16_01900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:157..159,aa:Leu,seq:cag)
     gene            complement(209..1969)
                     /locus_tag="PKI16_01905"
     CDS             complement(209..1969)
                     /locus_tag="PKI16_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNR53209.1"
                     /translation="MQGKKEKKEVLSMKDALSSLSDSEIKEITPQKDLRDFFWNYPST
                     ISKIFKTTHPFSYILLFITLGFVFILFLHSATFANILDGAEDKETFTEGSVGAISSFN
                     PIFASANYFDKSVESLVFDKFIHIDKDGKPIVGIAEKWSTTESKLEYIFTIKKDLLWQ
                     NGEKLTAQDVVFTFKTALSLSQDYNYDTVGISLEGVTVEQIDEYTVKFKLVEENPLFF
                     EAISIYIVPYSVLGDVNLGDMPFNIFARYPIGSGKYKVTRTEQNAVYLVDNEYDRYTP
                     NIKNITLRVYPDTKSLNTAFRTGLLDAVNGWDQEALSFTTEYPNFETYIMEEKYRSKL
                     MFFNLRKEDLKDENLRKGLSSLIDKDKLYEEAKISGVSIYGPYPPDSWVYNKDVDYLK
                     YSPEKAKAYLKSAGYERKEGGIYYESKENEILSFTLTYFQSESNDRLVTKLKELLELE
                     GVILKTERLNYTQITQEIIATRDFELLLFEVETTVDPDQYNLWHSLKSSYPDLNLSGY
                     SYERVDILLEDGRRTTNVNTRKARYVSFQKYLVEDAPVVFLYHPNFVYVVNDQLKGVN
                     LKQGNFSYERFANIESWYWK"
     gene            complement(1974..2207)
                     /gene="secG"
                     /locus_tag="PKI16_01910"
     CDS             complement(1974..2207)
                     /gene="secG"
                     /locus_tag="PKI16_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNR53210.1"
                     /translation="MNDNLLNLIYILQIVVCSLLVVSVLMQNRAEGLGKMFGGGGEVF
                     RTKRGLEKFLYYFTITLIVVLIVLSLLLLKLSA"
     gene            complement(2228..2638)
                     /locus_tag="PKI16_01915"
     CDS             complement(2228..2638)
                     /locus_tag="PKI16_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53211.1"
                     /translation="MRNTLRLFFVYSLILFVAMQIFERGIIFPSGGIYMFTTIIILAL
                     TLMVSPPLLNFLTIKSKFPTFFLMSTILLIGVMFLLKTFMTDFYIETYTFEQTNIGTL
                     QINSFEVTSLISIIGFSVVTSLFAALYRVLDNAE"
     gene            2737..4134
                     /gene="pyk"
                     /locus_tag="PKI16_01920"
     CDS             2737..4134
                     /gene="pyk"
                     /locus_tag="PKI16_01920"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNR53212.1"
                     /translation="MYNTAKTKIIATIGPSSWDKSVLTDMINSGLAIARINSSFANDE
                     EITRVAKSIREISPRVALMLDTQGSKVRIVNVKDEINIQGQTIISSNRNTQGIQISYP
                     NVHQYLKQNDLILLDDGNIELAVERIEGEEVFCKVIQSGVLKPNKTVFLPSVRLDLPI
                     ISEKDKGSIESALRNDYDFLSVSFVRTKGDIEEIKQIVGQSDIKIIAKIENQEGLDNF
                     DEILSVADGIMIARGDLGVEIPYEKLPVLQKQLIYRCRAVGKPIIVATQMLESMRENI
                     RPTRAEVSDVANAVMDGTDAVMLSAETSTGRYPVEAMKALQRIAKEAEAVMQTSKVYG
                     ATQASREVDELCRNACDIVENTNIKGIVVLSNMGKTLPSLCRHRPNIHIWSISNKIKR
                     IRQERIFFGVTSYYIREFPTDRDETIERAVDIVFSHGELDLNDKIAIISGSSIRHNSE
                     DATLEIVTVKDVLSR"
     gene            4138..4213
                     /locus_tag="PKI16_01925"
     tRNA            4138..4213
                     /locus_tag="PKI16_01925"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4172..4174,aa:Cys,seq:gca)
     gene            4262..4345
                     /locus_tag="PKI16_01930"
     tRNA            4262..4345
                     /locus_tag="PKI16_01930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4296..4298,aa:Leu,seq:taa)
     gene            complement(4348..4557)
                     /locus_tag="PKI16_01935"
     CDS             complement(4348..4557)
                     /locus_tag="PKI16_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53213.1"
                     /translation="MEDILKKNNTSNQPPGSEYEVKEPIEEGLECVWTRCKCLVCGYV
                     YEGAQECKKCPKCGNEDPDKFVDAE"
     gene            complement(4532..5149)
                     /locus_tag="PKI16_01940"
     CDS             complement(4532..5149)
                     /locus_tag="PKI16_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR53214.1"
                     /translation="MNKKVRSEELGYLFVVGGPGSSGSSTISVMLANEFSLRRVYGGK
                     ILRDIMSDLGFPSLESAYKEGNMKKVMEIDKKLDEYLIEASKTKNVLIESKVFAGIAK
                     KQNIPVTVSIWLDASLHVRTMRSLNKLDVTKPFQRIKEYLRIRKDLAYREKFDRRRYM
                     ELYKVDTAKPERYYDIVLNISRMNEEETFKLILKLIKDGGYIKEE"
     gene            5186..6379
                     /locus_tag="PKI16_01945"
     CDS             5186..6379
                     /locus_tag="PKI16_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNR53215.1"
                     /translation="MRKASRKNRIITALEGHKPDLLILIFAVMMTIFGAMMIFDASVY
                     KANTLFGDQFYFLKSQAVWIVIGTIPAVLIYFWDYRKFSKLALPFLIITIVLLVAVLI
                     LGEVTNGSKRWFAIGPLPKIQPAEFAKIAIILYLSSWLSSREYKYSTIKKAIQEGFLK
                     NLFTFLAILGIVAVLILLEPDLGTTVVICVTAFAMFIMAGNDRVHTLGSFASILVLLP
                     IGILAGILEPYRLKRIQTFFTLALTGEVADAKGSGYQMQQILIGIGSGGFLGKGFGQS
                     RQRFGYLVENTAFTDSIFAVILEELGLLGGAIIILSWVVFLWRGLRISMNAPDKQGRL
                     LAAGITVWLTSQTLLNIAANVGLVPLTGMPIPFLTYGGSSTIVTLIGIAILLNVSRFT
                     KTNAQ"
     gene            6369..7541
                     /locus_tag="PKI16_01950"
     CDS             6369..7541
                     /locus_tag="PKI16_01950"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNR53216.1"
                     /translation="MHSKKELKKKIVVTGGGSGGHLSAAEAIIKEISNRYDLTKKNFL
                     YIGGDLGMEGERIGSSIEQKRFRNAPFNCKYIRAGKLQRSFSLNTVKLLFRTFLGFYD
                     SYKILKKFTPDIVISTGGFVSVPVCVVAKLLKADIYLHEQTSTVGLSNRIVGSFSEKI
                     FLAYESSAQFFKKKKTIHVGNLVREDVLETKGSGKVVQALKKMIVNQEEYPIIYISGG
                     SLGSHILNDTVRSALHNLLNKYQIIIQTGDNKVYQDYDKLVKEKITLDDHLKDRFFPV
                     KYVESKEIGFLYDNIDLFVGRAGANTVYEVGVLKLPSIFIPIPWVTHNEQYENAKVLK
                     DLGLSEILPEGELTPENLVVKIDRYLSKERIVDTKKLKDAFPLDAAKKILQQIDLY"
     gene            complement(7538..8911)
                     /gene="murF"
                     /locus_tag="PKI16_01955"
     CDS             complement(7538..8911)
                     /gene="murF"
                     /locus_tag="PKI16_01955"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNR53217.1"
                     /translation="MKIYLKDIKERYPEFEIRNYDEGSFFVGFNHDSRCLLENEIYVP
                     IKGERFDGHDFIVEALDKGASMSICEKNSIEKISNVSKPVILVDSIEEGLQKIVNFAI
                     SDITAPIIAITGSTGKTTTKQMLAAILGFKNSVLYADHFNTVWGNAMLLSRYENEDFV
                     ILECAMDRKGEIAWHANTFDPDLGIILNIGHVHAEKIGSIEEIYEEKKNLADYLDRAG
                     KPLIINIDDERLRKLKDTFKSELLSYGKSDDAMFKIYEITLTDTGTDFNFGFINSWYT
                     VHINAFGEGMVYDAMAAIIAGYKLGISIDDCIRGILNFSPNSGRFEIEKFGKDIIVVN
                     DAYNANPSSMSMSLDTFTTMYTKDEYYRIVVLGDMKELGDVSEKMHKELGEKVKEMNF
                     NQIYYVGDMFDIFGIGKKLENADEVSSSLEQDLKRLSGRKVAVLLKASNSVGLSKIPE
                     YLRKLGL"
     gene            complement(8926..9321)
                     /locus_tag="PKI16_01960"
     CDS             complement(8926..9321)
                     /locus_tag="PKI16_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53218.1"
                     /translation="MPDSIGVPSFDEGLDVDALHEGEGNEMTPEEIKEVDAKFQELYP
                     DVHEIALKSFSEKMGVVDLVCPRAVVGGSQEIFMTPSLYGYNVENDDMKSANLRYIFP
                     EGLLTSEQRKAIDDIARNYNPNREKRPTI"
     gene            9621..10064
                     /gene="mraZ"
                     /locus_tag="PKI16_01965"
     CDS             9621..10064
                     /gene="mraZ"
                     /locus_tag="PKI16_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNR53219.1"
                     /translation="MLIGEYTTKIGEKKRVSLPKKFRDELGENLILTRGYEEALVLVN
                     QEMWSKVAEDVMNGSFINKNIRDTSRFLVGGAKEIATDTQGRFIIPESLFDYAQLDKE
                     IVFIGLVNWIEIWDKQKWEQRLQFLKANSEQIAQELSKMNETKNG"
     gene            10057..10944
                     /gene="rsmH"
                     /locus_tag="PKI16_01970"
     CDS             10057..10944
                     /gene="rsmH"
                     /locus_tag="PKI16_01970"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010688732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNR53220.1"
                     /translation="MDKIHKPVLLKESLESLNIIPDGFYIDCTLGDGGHSFEIFSRLS
                     DKGLLLSIDQDQYAIDFVKDFYKENIKENWIIKRSNFRKIDELVSQLNRKPNGILLDL
                     GLSSRQLELSHDRGFSYKEESEPLDMRMNPDFGVTAKDLLNALSEKQLTNLFREYGEE
                     KFAHRIAKEIKKAENPLETVGDLTRLIYKASPADNSNKNPSRRVFQALRIVVNDELES
                     LQEALDKSFEILQSGGRISVISFHSLEDRIVKNFFNNKARSGEGKLLFESHIAPTDEE
                     RRDNPRSASAKLRTIIKKK"
     gene            10941..11240
                     /locus_tag="PKI16_01975"
     CDS             10941..11240
                     /locus_tag="PKI16_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53221.1"
                     /translation="MKNLSTKNIPVIVLLVSVFCFAISQLAINSVLSPMGLELQSLNS
                     EKNYLVEENRSMEEQIAKSNSIIVIQKLAAKELKISSNNSKSIVYIENTNIIAEK"
     gene            11240..13072
                     /locus_tag="PKI16_01980"
     CDS             11240..13072
                     /locus_tag="PKI16_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNR53222.1"
                     /translation="MASLHTLYSSQGKTHKKGSTKKRLDNIYLMGIGLTVFAFIVLLQ
                     IFRWQILEADKFKSLANEQYKTNQIQTAQRGTITASDGTVLAVDEPTWNIYATLSTDP
                     VERATFFANKEKFIAVVSTTLGVEREEIESKITDSFVYAPIKKNVTTEKKKALETANI
                     FGEGTEGFGLYFESEERRIYPNGQLAAHVLGFIGQNEEGTQIGQYGLQGYYFGDITGR
                     EGYSYEEKDSTGNVILTSEYEPILPREGKDFKLTIVPNIQSKVEQKLEEGVKKTRSKS
                     GTAILMDPKTGAIMAMANYPSYNPNEYWRVSEPWILKNRAISDVYEYGSVQKPITIAI
                     ALESGAIDKDFKCVDKTGSLDLYKATKYPDLKGRFIYTWNKKADGTLDISGILQNSNN
                     PCVAQIALKTNFADFYSKLKDFGIGEFIGVGLQEETTSYLRPYEQWTKLDLITASFGQ
                     GISATSLQVISALSTLANDGKRMRPYIISEITDNKETIVIEPKVISEPVSKDTANFVT
                     KALQEAVQKGLLGGLVSPDLKKYDIAAKTGTAQIAKSDSLGYSKYITNATTVGYAPTD
                     SPKLILLVKLEEPEISEFAAYTSNLVWRDIFLAIVNDMEIQKRK"
     gene            13105..14133
                     /locus_tag="PKI16_01985"
     CDS             13105..14133
                     /locus_tag="PKI16_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53223.1"
                     /translation="MIDILKEGLNALFISVIASLIFAPIMITILYKFNQVSGIKKSKI
                     GGGDGDNTLFMRIMNVTKTNGTPNMGGILIWILVPILCYFLVPLTPVVKIFLLGFLLY
                     GLWGFIDVVIFTNGFKNNARMRAFQETFEWRLGKLAFATVMNIIIMFLFYRTGAFESI
                     SFLNIATITVTPLIVILTGGLCQLAIYSAELTDGLDGLMIGIFSIINMSMITLLVIQG
                     KNEFIPILLILLGVQLIDLYFNIPPARFWNGGPGAMPLGFTMFFVALVTDNLLPYFAM
                     SSITWGIMASSAIQIISMKFFKRRVFRIAPIHHHFQAKGWPQYKVTMRFWLFTAITGI
                     IGIYLGIL"
     gene            complement(14130..14831)
                     /locus_tag="PKI16_01990"
     CDS             complement(14130..14831)
                     /locus_tag="PKI16_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53224.1"
                     /translation="MGKLKQNNWLVVITIFLVVSSVIALSISMRKSLGFGTCANDEEI
                     YLSGQLIPNYNGRTDCYCSKSGNVQCNSDSTLISLENYSSAGLKYFSSFLNYLDKEEL
                     DTDNIKAVDVGYTSGVLTVVIEREAMCGQEQESPVQVGFYKYSENDLTLTVLTNRDPS
                     FYSKVCKIDGKFVLTKEDLFLTDDFKLYFQNDLGQIFDLGVCVYKEKLYGNGDVLQSE
                     AGQACSCNFGIVECD"
     gene            14922..15800
                     /locus_tag="PKI16_01995"
     CDS             14922..15800
                     /locus_tag="PKI16_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNR53225.1"
                     /translation="MHDSHIHLNLCPLQENIEQVINDFQKENGKHILTQSTDLLDYKE
                     NLDISKRYPDIVQLALGLHPTVFEEYTVQRNIFKDIQKKSLKMIADFEDIFEKNILAI
                     SAVGETGLDYYQFNNDQTVDEDIKEELCEIQKMSFTKHIQLALKHNLPMSIHARDMNG
                     STECLEDVLRIVAQEGRGQLKGSFHSYTGNLDFLTDILDMGFYIGFNGIITYKSGENV
                     RDILKEVPLERILFETDGPFLPPQSVRKDKKLKEKYAQPKDIKEIIQTACEVKNITYE
                     KMERVTDENYERLFVL"
     gene            complement(15786..16472)
                     /gene="rsmI"
                     /locus_tag="PKI16_02000"
     CDS             complement(15786..16472)
                     /gene="rsmI"
                     /locus_tag="PKI16_02000"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNR53226.1"
                     /translation="MNKGKLYVVATPIGNLEDITFRAIDTLKKVTFVLAEDTRESYKL
                     FNRYDIKTQLISYRDQNHEKMLGKILEKLDMGLDLALISDCGTPLISDPGFKLIRELR
                     ERKYDVVSIPGPSALTSALSISGLVTDKFTFLGFLPKSNVRRKKLLQEYVDIEGSLIF
                     YESPQRLIKLLDMLKNVFGEKKVGIAKDISKMREMFFYGNISEVSKEIETDDFKENPH
                     GEYVVILENK"
     gene            complement(16465..17172)
                     /gene="truB"
                     /locus_tag="PKI16_02005"
     CDS             complement(16465..17172)
                     /gene="truB"
                     /locus_tag="PKI16_02005"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNR53227.1"
                     /translation="MIDGILVIDKQEGITSYTVINKLKKVLPRGQKIGHAGTLDPFAT
                     GALPILLGKATKLMNKIHEFEKEYLVTAEFGYMTDTQDGTGEILKKENKPTPLERGVV
                     EKIIKENFIGNISQLPPRYSAKKVSGVRAYDLAREGKEFELMPKDISVGEFEIVGYNW
                     PLVKFRIVCSTGTYIRTLINDLGIKLGTYATAIELRRTYVGPFKVEDSISSEDIKEGM
                     NSSIIDLDKVNQMISNE"
     gene            17211..17750
                     /locus_tag="PKI16_02010"
     CDS             17211..17750
                     /locus_tag="PKI16_02010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR53228.1"
                     /translation="MLNGITLGIIIVITIFVFLFIISLLTNILLAPVIRTPKKVIQEI
                     LEVMNLTKEDTFVDFGCGDGRLVLGAYEQAKCKCIGYDISPIMLMLAKTARVVRYPLS
                     KDIIFEAQDIFQVDISNVTKIYCFLDEKSMNILNEKLSNFVKGGGEVYSYMYNIKGLK
                     NEKKIILSNGNTLYMYRGV"
     gene            complement(17801..18133)
                     /locus_tag="PKI16_02015"
     CDS             complement(17801..18133)
                     /locus_tag="PKI16_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53229.1"
                     /translation="MIAEILVNPLLVFAVVLIVVELREHGSNYGKRQIFFNILMVLAI
                     VAAVINLPESLYPAEKLAIARTIIIAIALLLGFGDLFHLPKETVVINVGEIALLSFAL
                     SLQASMLL"
     gene            18363..19568
                     /locus_tag="PKI16_02020"
     CDS             18363..19568
                     /locus_tag="PKI16_02020"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNR53230.1"
                     /translation="MPIYEPLASKYRPKTLDDFIGQQHLVGENGPIRKFLKTGIIPSM
                     IFWGPPGTGKTTLAYIISLNLYYDFHKLQAVSAGKDALRKIIKIALNNQQYGKKTILF
                     LDEIHRWNKAQQDALLPYVEKGVIILIGATTENPSFTVISALLSRTKVFVFNQQTEED
                     IYKLLKTILKKEYPNIKVKKKVLGMMSNLSNGDIRSAMNILEMATTLVQEEKVSEITE
                     EILKNTIQKPVYYDKDGEEHFNIISAIHKSMRSSNPDAAVYWVGRMLAGGEDPLYVAR
                     RLLRFASEDVGNADPQALILANSVYEACQKLGMPECQIFLTQLAIYLSKSPKDNTAYV
                     VENMVKKDIEEYGNLPVPLNIRNAETKLMKDIGYGKGYQYDHDLPNKKSEQQCLPDRL
                     KSRKYIKDN"
     gene            complement(19565..20032)
                     /locus_tag="PKI16_02025"
     CDS             complement(19565..20032)
                     /locus_tag="PKI16_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53231.1"
                     /translation="MITGIEKMENGVIDFNGKLKSFEEIFFDTDIVPNSRYRLVENTE
                     KANPGYCNGILNEYEAQILREALCDVDPVSSSDILDLSVNTETVFKKNIENFDLRGIQ
                     SKRLIFNLGGNSFRGMSGGDSPEERNIQALLLSIMGIIERRIRNTTLIEFRIV"
     gene            complement(20034..20564)
                     /locus_tag="PKI16_02030"
     CDS             complement(20034..20564)
                     /locus_tag="PKI16_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53232.1"
                     /translation="MSAGCKSERERERDPFEGIKELKDILELNEIGRLIIAEESLESP
                     MSDEDAQNLKSLLGEKYDELGLDIPEEGTKVDLSISFSHILPKYKGKKSSRDGIKYPV
                     TSLDLGIGWGCNYLYARVYDYENYDGYKMVNIGLNNHPRHIYLKFQGERKLAFAPVFL
                     LVSDIIRSKIGSEKEE"
     gene            20628..21851
                     /gene="tyrS"
                     /locus_tag="PKI16_02035"
     CDS             20628..21851
                     /gene="tyrS"
                     /locus_tag="PKI16_02035"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNR53233.1"
                     /translation="MTSIKTDTEIIERILNKGVEQILPSKDTLKEKLLSGQRLNIYQG
                     FDPTAETLHIGHSVGMRKLEDFRKLGHHVIFLIGDFTARIGDPSDKMATRKMLTEEEV
                     KKNMEKYVEQASHIIDMNNSENPVEIMYNSKWHNELKFGEIINLASEFTVQQMLKRSM
                     FQKRLEEDKPIHLNEFLYPLMQGYDSVMMDIDIEVGGNDQLFNMLAGRDMVLNHLKKE
                     KIVLAGKLLESNDGVKMGKSMNNMIRMDDKPEDIFGKVMAFADDLIVIGFEILTSASM
                     QEVETYKNRLDADENPINLKKELAYRVTSEITSEEDAKKAETYFENIYQNKRDDENIP
                     EKEITKESIPLVDLLLECGLTKSKSQARRLIKQGAVKIDNEKQSDWNLVISLSQKNTF
                     TLKVGRQLCRILYKK"
     gene            21913..23364
                     /locus_tag="PKI16_02040"
     CDS             21913..23364
                     /locus_tag="PKI16_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR53234.1"
                     /translation="MNKIENLKAEILNNSPENYKSLVNDAIKFAQEAHEGESRKSGED
                     FIIHPLNTALTVAKIGLDTDTIIASLLHSLAHKEDKMNEIKEIFGEDVYNLITQTRKI
                     HDATKNSRTHDEVITKYILNRSKDIRPIIIKLASALHNVQTIEHLPDEELKDTLRKIF
                     NIYANLAEYLNFNTIKKELEENAFKAYQPVEYESITEKMESLNINDELLNRYREVITK
                     ELTDIPVKVEGRIKGKYSIYNKLKKYEKEWKDPNITSLTDIIAFRVITQNMDDCFLAL
                     EKIMDNAELNTELFKDYISNPKPNGYQAIHSIVKFPEISNLEIEVQILTEVMHYTNTY
                     GRASHIAYKASQSRFARPTDVYSWVEDVHNKIESHISKRNLKRSIPIECNIFENEVYA
                     FTPKGEIIPLTKGDTALDFAFKVHTQIGNSAIGAKINGIAGKLDTVLKTDDVVEIKTQ
                     KDKKWQNESALKIVNSSSSRTKILQGICKKNKN"
     gene            23492..25489
                     /locus_tag="PKI16_02045"
     CDS             23492..25489
                     /locus_tag="PKI16_02045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNR53235.1"
                     /translation="MPLTADSQITEIKGIGESLATKFNHLNINTVKDLLFHVPIKYQD
                     SSHLLPIEEFLLEGEGTFLAEIVDIKTFRTRSRKTITIVKVKDDTKKINLTFFNQPYL
                     SKALIKNEIYLFDGKVTIKGSKRDIYNPKYEIFKGEKEKQTNLGKLAPIYPETEGLTS
                     NMIRKILKTVKTNIPEIISDPITSYTETSLQEALSKIHFPKDTEDILIARKRLAFDEM
                     LRIAFKIEQEIEERSTQVALPMKTNSKILNEFIKSLPYKLTDDQNKSLEVILQELNSN
                     IPMNRLLNGDVGSGKTVVAAGAILNTIKNGFSSILLAPTTVLAKQHYDTFKELFKGLH
                     IPIELCISSDQNISDANNKLIIATHSILYNKELPTDLNLVIIDEQHRFGVEQRKYFLK
                     KLPHTPHHLTMTATPIPRSLTEIFFGNMDVSEIREKPTNRKEIKSYYTPFAKRDDCFN
                     WIADKIETSEKKEQAFIIYPLIEETEVSVAKTVLASFEELKKKYFKDIPIIYLHGRMK
                     DSEKNKILNDFKKKKYNVLVSTSVIEVGIDIQDATIMVIENAERFGLAQLHQLRGRVG
                     RSDKQSYCYAIPGIEVAKDSKAEERLKYFSKHSSGFDVAEYDLQSRGPGEVYGIAQSG
                     IPNFKVASIHDLDTLKKARNVAKRLLKSNNQIENILENIFR"
     gene            25486..25941
                     /locus_tag="PKI16_02050"
     CDS             25486..25941
                     /locus_tag="PKI16_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNR53236.1"
                     /translation="MKIHIVLDNIRSVFNVGSIFRSADGAGSVEKIYLCGMTTPIDNP
                     KLEKTALGATEMVDSEHYDTTQEALDELREKNIPIYSVELTDDAQNFQKVEYPEELAL
                     VFGHEKNGVSADILQESDKKIYIPMRGKKESLNVANCASIILYEITRKS"
BASE COUNT         9314 a         4731 c         4309 g         7620 t
ORIGIN      
        1 taagagattt caaaaacttt gacccagaca atgcaaaggg caaagaaagc aacaatcctc
       61 agaggaaaat taagatatct ctcacatcaa aaaaggatta attcgtgata gaatgtagca
      121 catgccagga tggtgaaatg gcagacacgc tacgttcaga acgtagtcct agcaataggg
      181 tgggggttca actccccctc ttggcatgtt atttccaata ccaactttcg atatttgcaa
      241 atctttcata ggagaagttc ccctgcttta gattcacacc ttttaactgg tcgtttacta
      301 cataaacaaa gtttggatga tacagaaata caacaggagc gtcttctacc aaatattttt
      361 ggaaggatac ataccttgct tttctagtat taacatttgt agtccttctt ccatcctcca
      421 ggagtatatc tactctctca tatgaatatc ccgacagatt aagatctgga taactactct
      481 tcagagagtg ccaaagattg tattgatcgg gatctactgt agtttcaact tcaaacaaaa
      541 gtaattcaaa atctcttgta gcaataatct cttgggtaat ctgtgtgtaa tttaatctct
      601 cagttttaag gataactccc tccaattcaa gtaattcttt caattttgta actaacctgt
      661 cattactctc gctctggaag taagtcaaag tgaagcttaa aatttcattc tccttggatt
      721 cataataaat accaccttct tttctttcat agcctgcact tttaagatat gcctttgctt
      781 tctctggact atatttaaga tagtctacat ccttgttgta aacccaagaa tctgggggat
      841 atggaccata tattgagaca ccagatatct tagcctcttc atataattta tctttatcaa
      901 ttaaagaaga taatcctttt cttaaattct catcctttaa atcttctttc ctcaaattga
      961 agaacatcag tttgcttctg tatttctctt ccataatata tgtctcgaag tttggatatt
     1021 ctgtagtaaa gctcaatgcc tcctgatccc aaccattaac agcatccaag agtccagttc
     1081 taaatgctgt gtttagactc tttgtatctg gatataccct taaagtaata ttcttaatat
     1141 ttggtgtgta tctgtcatac tcgttatcta caagataaac tgcattctgt tccgttctgg
     1201 ttactttata ctttccacta ccaataggat atcttgcgaa aatattaaac ggcatatctc
     1261 cgagatttac atctcctaaa acagagtagg gaactatata tattgatata gcttcaaaga
     1321 atagaggatt ttcttcaact aatttaaatt taactgtata ttcatcaatt tgctccaccg
     1381 ttacaccctc taaagaaata cctacggtat cgtagttgta atcttgagat agagaaagtg
     1441 ctgttttaaa tgtaaataca acatcttggg ccgtaagttt ctccccattt tgccaaagta
     1501 agtccttttt aattgtaaaa atatattcta atttactctc agtcgtactc catttctcag
     1561 caattcctac aattggtttc ccatctttat caatatggat aaatttatca aaaaccaaag
     1621 attctacaga tttatcaaaa taatttgcag aagcaaaaat aggattaaaa gatgaaattg
     1681 caccaacact accctcggtg aaagtctcct tgtcctctgc accatctaat atatttgcaa
     1741 atgtagcaga gtgaagaaaa aggataaaaa caaagcctaa agtaataaac aataaaatgt
     1801 aactaaaagg atgggttgtt ttaaaaatct tggatattgt agaaggataa ttccaaaaaa
     1861 aatctcttaa gtctttttga ggagtaatct cttttatctc agaatccgaa agagaggata
     1921 gtgcatcttt cattgagaga acttccttct tttctttttt tccttgcata tttctaagca
     1981 gataatttca gtaacaaaag ggaaagaact attaatacca caattaaagt aatggtaaag
     2041 tagtaaagga atttctccaa gcccctcttt gttctaaata cctctccacc accaccaaac
     2101 atctttccaa ggccctcagc acgattttgc ataagaacac tcacgacaag gagagaacat
     2161 accacaatct gcaatatata tattagattt agaagattat cattcataat ggctattata
     2221 caccatttta ctctgcatta tccaagactc tatacagagc tgcaaacagt gatgtaacaa
     2281 cagaaaaacc gattatagat atcaagctgg ttacttcgaa actgtttatc tgtaatgtcc
     2341 caatattcgt ttgctcaaag gtatatgttt caatgtagaa atctgtcatg aatgtcttta
     2401 gaagaaacat tactccaata agaagaatgg tagacatcaa aaagaaggtc ggaaacttgc
     2461 tttttatagt aagaaaatta agtaaaggag gagataccat gagagtcaat gcaagaataa
     2521 ttatcgtagt aaacatatag atacccccag atggaaatat gattcctcgc tcaaaaattt
     2581 gcatagcaac aaataagatt agggagtaaa caaaaaataa acgtagggta tttctcataa
     2641 aaaggcagta aatattttat ctttgtataa tatatcacaa cttatcatag acatgaacta
     2701 tttctttgag tatactttac ctaataaaac tgccgtatgt ataacactgc caaaacaaaa
     2761 atcattgcta cgataggacc atcctcatgg gataaatctg tgcttacaga catgataaac
     2821 agtggtcttg ccattgcaag aataaatagt tcatttgcta atgacgaaga aatcaccaga
     2881 gttgcaaaat caataagaga aatatcccca agagtggctt tgatgttgga tactcaagga
     2941 agtaaggtta gaattgtaaa tgtaaaagac gaaattaata tccagggaca aacaattatc
     3001 tcttccaatc gtaataccca aggcattcaa atttcgtatc ctaatgttca tcaatacctt
     3061 aaacaaaacg acttaatact acttgatgat gggaatattg aattagctgt agaacgaata
     3121 gagggagaag aagtcttctg taaagtaata caaagtggag ttctaaaacc aaacaaaaca
     3181 gtattcttgc cttctgttag attagatctt ccaataattt cagaaaaaga caaaggctcc
     3241 atagagtctg cattacgcaa tgattatgat tttctttctg tatcttttgt aagaacaaaa
     3301 ggagatattg aagagatcaa acaaattgtt gggcaatcag atataaagat aattgcaaag
     3361 atagaaaatc aggaaggact tgataacttt gatgaaatat taagtgttgc tgacggcatt
     3421 atgatagcaa gaggagatct tggagtagaa ataccctatg agaagcttcc agtactccag
     3481 aaacaattaa tatacagatg cagagctgta ggaaaaccta taatagtcgc aacccagatg
     3541 ttggaatcca tgagagagaa tattagacca actagagcag aggtttctga tgtagcaaat
     3601 gctgtcatgg atggaacaga tgcagttatg ctctccgcag agacctctac gggtagatat
     3661 ccagttgaag caatgaaagc attacagaga atagccaaag aggcagaagc agttatgcag
     3721 acttcaaagg tatatggagc aacccaagct tcaagagagg tagatgaact ttgtagaaat
     3781 gcttgtgata ttgttgaaaa tacgaatatt aaaggaatcg tggtcctttc aaatatgggt
     3841 aaaacactac ctagcctttg tagacataga ccgaacatcc atatatggtc tataagcaat
     3901 aaaataaagc gaattagaca ggagagaata ttttttgggg ttacaagcta ttacataaga
     3961 gaatttccaa cggatagaga tgaaacgata gaaagggcag tagatattgt tttctctcac
     4021 ggggagttgg atttaaacga taaaattgct attattagcg gtagttctat aagacataat
     4081 agtgaagatg caacattaga gattgtaact gtaaaggatg tcttatctag atagcgcggt
     4141 gacgtagcca agttggttaa ggccgcggtc tgcaaaaccg ctatcacggg ttcgaatccc
     4201 gtcgtcacct ccatatttat tgatatttaa gaacttttaa aatagaatac tatgaaaata
     4261 agccgggatg gcggaatagg cagacgcgcg ggacttaaaa tcccgttcta gaaatagagt
     4321 gagggttcga ttccctcttc cggcagatta ttcagcatca acaaatttat ctggatcttc
     4381 attaccacac ttaggacact tcttacactc ctgtgccccc tcatatacat aaccacacac
     4441 taaacactta catctggtcc atacacactc taacccttcc tctattggtt cttttacctc
     4501 atattcagag cccggtggtt gattagaagt attattcttc tttaatatat cctccatctt
     4561 taattaactt taaaattaat ttaaatgttt cctcctcatt catgcgagaa atatttagaa
     4621 caatatcata gtatctttct ggtttagcag tatccacctt atataattcc atatatctcc
     4681 ttcggtcaaa tttctctcta tacgctaaat ctttcctaat cctgagatat tccttaatcc
     4741 tctgaaatgg ctttgttaca tctaatttgt ttaaacttct catcgtgcga acatgtaagg
     4801 aagcatctaa ccatatacta actgttacag gaatattctg tttttttgct ataccagcaa
     4861 acactttgga ttctataagg acattttttg ttttggaagc ctctattaaa tattcatcca
     4921 actttttatc tatctccata actttcttca tattcccctc cttgtatgca ctctcaagtg
     4981 aaggaaatcc taaatctgac ataatatccc gaagtatttt accaccatac accctcctta
     5041 gagaaaattc gtttgccaac attacagaga tagtagagga accgctactt ccaggtcctc
     5101 caaccacaaa aagataccct aattcttcag atctaacctt tttattcata tattaatata
     5161 gtatattaat aatcatacta tgtctatgag aaaagcttcc agaaaaaaca ggattataac
     5221 tgctttagaa ggacacaaac ctgatttact aatccttata tttgcagtca tgatgacaat
     5281 atttggtgct atgatgatat ttgacgctag tgtgtacaaa gcaaatacac tttttggtga
     5341 tcagttttac tttcttaaat cacaagctgt atggattgta attggtacta tccctgcagt
     5401 attaatatat ttctgggatt acagaaagtt ctctaaacta gcactacctt tcctaatcat
     5461 cacaatcgtt ctactggtag cagttttaat acttggtgaa gtaacaaacg gctccaagag
     5521 atggtttgct attggtcctc taccaaagat acaacctgca gaattcgcaa agattgccat
     5581 aattctctat ctttcctctt ggctttcttc aagggaatat aaatacagta cgattaagaa
     5641 agctatacag gaaggtttct taaaaaatct ttttacattc ttagccatac ttggaatagt
     5701 agctgtttta attctcttag agcctgactt aggaacaacc gttgtaatat gcgttacagc
     5761 atttgccatg ttcattatgg caggaaacga cagggttcat acactcggtt cgtttgcttc
     5821 aattcttgtt ctgcttccaa ttggtattct tgcagggata ttggagcctt acagattaaa
     5881 gagaatccaa acttttttta cattagcact gacaggtgaa gttgcagatg caaaaggttc
     5941 aggataccaa atgcaacaga tattaatagg cataggatcg gggggattcc ttggaaaagg
     6001 atttggtcaa tcaagacaaa ggtttggata cttggtagaa aatacggcat ttacagattc
     6061 tatttttgca gtaatactag aagaattagg cttactagga ggtgcaatta ttattctctc
     6121 ttgggtagta ttcctttgga gaggacttag aatctctatg aatgccccag acaaacaagg
     6181 cagacttctt gcagcaggaa taaccgtatg gctgacatct cagacactat taaatattgc
     6241 tgctaatgta gggttagttc ctctcacagg aatgcctata ccattcctta catatggtgg
     6301 ctcaagtact attgtaaccc tcatcggtat tgctatacta ttaaacgtta gtagattcac
     6361 aaaaacaaat gcacagtaaa aaagaattaa aaaagaaaat tgtggtaacc ggaggtggtt
     6421 ctggcggaca tctttctgca gcagaagcta ttattaaaga gatttctaat agatacgatc
     6481 ttaccaaaaa gaatttcttg tatatcggtg gcgatttagg aatggaagga gagagaattg
     6541 gtagctccat agagcaaaaa aggtttagga atgccccctt caattgtaaa tatatacgag
     6601 caggtaaatt acaaagatcg ttttctttaa atacagttaa attactattt agaacttttc
     6661 ttggcttcta tgactcatat aaaatattaa agaaatttac accagatatt gtaatctcta
     6721 ctggagggtt tgtatctgtt ccagtctgtg tagtcgcaaa gcttcttaaa gctgacatat
     6781 atctgcatga acaaacttct acggtaggac tctcaaatag aatagttgga agtttttctg
     6841 aaaaaatctt tttagcctat gaatcttccg cacagttttt taaaaagaaa aaaactatcc
     6901 atgttgggaa tctcgttaga gaggatgtcc ttgagaccaa aggaagtggt aaagtagttc
     6961 aagccctaaa gaaaatgata gttaaccaag aggaatatcc aattatatat atctctggtg
     7021 gaagtttggg ttcacacatc ttaaatgata ctgtaagaag tgcattgcac aacctactaa
     7081 ataagtatca gataatcata caaacaggag ataacaaggt ataccaagac tatgacaaac
     7141 ttgttaaaga aaagattacc ctagatgacc atcttaagga taggttcttc ccagtaaaat
     7201 atgtcgaaag taaagaaatt ggctttcttt atgacaatat agatttgttc gttggaagag
     7261 ctggagcaaa tacagtttat gaagtagggg tattaaagct tccgtccata ttcataccaa
     7321 taccttgggt tacacataat gaacagtatg agaatgcaaa agtattaaag gatcttggtc
     7381 tctctgagat tcttccagag ggagaactta caccagagaa tttagtcgta aagattgacc
     7441 gatatctctc aaaagagagg attgtcgata ctaagaaact aaaggatgca ttcccactgg
     7501 atgcagcaaa gaagatatta caacaaatag atttatacta gagacccaat tttcttaaat
     7561 actccggtat tttactcaat ccgacactat tacttgcctt caacagtact gctacttttc
     7621 ttccacttaa tctctttaaa tcctgctcta gagaagaaga aacctcatct gcattctcta
     7681 gcttcttacc aataccaaaa atatcaaaca tatctcctac ataatagatt tggttgaaat
     7741 tcatctcttt taccttctct ccaagctctt tgtgcatctt ctctgaaaca tctcccaatt
     7801 ctttcatatc tcccaatacc actattctgt agtattcatc ctttgtatac atagttgtaa
     7861 atgtatccaa cgacatactc atagaagatg gattagcatt gtaagcatcg tttacaacaa
     7921 tgatatcctt tccaaatttt tcaatttcaa atctaccact atttggacta aagtttagaa
     7981 ttcctctaat acaatcatca atacttatcc ctagcttgta acctgcgata attgctgcca
     8041 ttgcatcgta aaccataccc tctccaaaag catttatatg tacagtatac caagagttaa
     8101 taaacccaaa attgaaatct gttcctgtat ctgtaagagt tatttcataa atcttaaaca
     8161 ttgcatcatc acttttacca tagctaagca gttcggactt aaaagtatct tttagctttc
     8221 gcaatctctc atcatcaata tttataatca atggcttacc agctctatct aaataatctg
     8281 caagattttt cttctcttca tagatttcct ctatacttcc tatcttctct gcatgtacat
     8341 gaccaatgtt caatatgatt ccaagatcag ggtcaaatgt atttgcatgc caagctattt
     8401 ctccctttct atccatcgca cattctaaaa ttacaaagtc ctcattctca tacctactga
     8461 gtaacatagc gtttccccat acagtattga aatggtctgc atacaaaaca gagtttttaa
     8521 aacccaatat tgctgctaac atttgtttag tagttgtttt acccgtacta cctgttatgg
     8581 caataatagg ggctgtaata tctgatatag caaaatttac aatcttttgc agaccttctt
     8641 ctatactatc aacaagaatt actggttttg aaacatttga gattttttct atactatttt
     8701 tctcacatat tgacatagat gcacctttat ctagcgcttc aacaataaag tcatgcccgt
     8761 cgaatctctc tcctttgata ggtacatata tttcattttc tagtaaacat cttgaatcat
     8821 ggttaaaacc aacaaagaaa gacccttcat cataattgcg aatttcaaat tcaggatatc
     8881 tttcttttat atctttaaga tatatcttca ttttattttt ctaatctaaa tggttggacg
     8941 cttctcacga ttgggattgt aatttctagc tatatcgtca atagcttttc gttgctcgga
     9001 tgttaacaat ccctctggaa aaatatatcg aagatttgcg gatttcatgt catcattttc
     9061 tacattataa ccataaagag acggtgtcat gaaaatctct tgggatccac caacaacagc
     9121 cctaggacaa acaagatcca caactcccat cttctcactg aaagatttaa gagctatctc
     9181 atgtacatca ggatataatt cttgaaattt ggcatctact tccttaatct cttctggtgt
     9241 catttcattt ccctcgcctt cgtgtaaagc atcaacatct aatccttcat caaaactcgg
     9301 tactcctatt gaatctggca tttttgttta ttattaattt atatattatc tattataccc
     9361 aagcgaataa ttagcacaac ccccagtaaa aagctttatt catacaagca tttttaatat
     9421 atattctaat aataatggct atcggttcac aatcgatatg tatatattaa tttcgatttt
     9481 tcttttctac agtgacaaag cacatatcct agagcattat atttgagcct gtagtggttt
     9541 tttcttgact cattgtatag caaatgttac attagtggca agaagtggta aaaagtggtg
     9601 aaaggtgata tattaaaaac atgctaatag gagagtacac aacaaagata ggagagaaaa
     9661 agagagtatc acttcccaaa aagttcagag atgaactagg tgagaattta attctcacca
     9721 gaggttacga agaagcttta gttcttgtaa accaagaaat gtggagcaaa gtggccgaag
     9781 atgtaatgaa tggctctttt ataaacaaaa acatccgtga taccagtaga ttcttagtag
     9841 gaggcgcgaa agagatagct acagatacac agggaagatt cataattcct gagtctcttt
     9901 ttgattatgc acaattagat aaagaaattg tctttatcgg attggtcaat tggattgaaa
     9961 tatgggacaa acagaagtgg gaacagagac ttcagttcct gaaagccaat agtgagcaga
    10021 ttgcacaaga gttaagtaag atgaacgaaa caaagaatgg ataagataca caagccagta
    10081 ctattaaaag aatctcttga atccttaaat attattccag atggatttta catagattgt
    10141 acacttggag atggaggaca tagctttgag atattctcga gactctcaga taaaggctta
    10201 ctccttagca ttgatcaaga tcaatacgca atagactttg taaaagactt ctacaaagag
    10261 aatatcaagg agaactggat cataaaaaga tcaaatttca ggaaaataga tgaactagta
    10321 tcgcagttaa acagaaaacc aaacggcatt cttctagatc ttggtctatc ttctcgccaa
    10381 ttggaacttt cccatgacag gggtttcagt tacaaagaag agtcagagcc attagatatg
    10441 agaatgaatc cagattttgg tgtaactgca aaagatcttc ttaatgcact ctccgagaaa
    10501 caattaacaa atctcttcag agagtacgga gaagagaaat ttgctcacag gatagcaaaa
    10561 gaaattaaga aagcagaaaa tccactagaa acagttggag atctcacaag acttatctac
    10621 aaagcatcgc cggcagataa ttctaacaag aacccttctc gcagggtctt tcaggccctg
    10681 cggatcgtgg taaatgatga attagaaagc ttgcaagaag cattagataa atcttttgag
    10741 atcttacaat ctggaggaag aatatcagta atatccttcc actcattgga agatcgcatt
    10801 gtaaaaaatt tttttaacaa caaagcaaga agcggtgaag gtaaattact tttcgaaagt
    10861 catattgccc caaccgacga agagagaagg gataatccta gatccgcttc tgccaagttg
    10921 agaaccataa ttaaaaagaa atgaaaaacc tgtctacaaa aaatatcccc gtcatagtac
    10981 tactagtctc agtcttttgt tttgctatat ctcaattggc aataaactct gttctgagcc
    11041 caatgggatt agaactacaa agtttaaaca gtgaaaaaaa ctatttagtg gaagaaaata
    11101 gatccatgga agaacagatt gcaaaatcca attccataat cgtaatacaa aaattagcag
    11161 caaaagaatt aaagatatct tcaaataaca gcaaatctat tgtatacata gagaatacaa
    11221 atataattgc tgaaaaataa tggctagctt acacaccttg tattcttcgc agggaaaaac
    11281 tcataaaaaa ggtagtacaa aaaaaaggct agacaatatc tacctcatgg gtatagggct
    11341 tactgtattt gcattcattg tgcttcttca aatttttagg tggcaaatat tggaagcaga
    11401 caaatttaaa agtttagcca acgaacaata caaaacaaac cagatacaaa cagctcaaag
    11461 aggaacaata acggcatcag atggaaccgt cctagcagta gatgagccaa catggaatat
    11521 atatgcaact ttaagcacag atccagtaga aagggcaacc ttctttgcca acaaggaaaa
    11581 attcatcgca gtagtttcaa ctacactagg agtagaacgt gaagaaattg agtccaagat
    11641 tacagactca tttgtttatg caccaatcaa aaagaatgtt acaacagaaa aaaagaaagc
    11701 ccttgaaact gcaaatatct ttggagaagg tacagagggt ttcggattgt attttgaaag
    11761 tgaagaacga aggatatatc caaatggaca gttagctgca catgtactcg ggtttattgg
    11821 gcaaaacgaa gaaggcacac agattggaca gtacggactt cagggatact actttggaga
    11881 tataacagga agagaaggat attcatacga agagaaagat tctactggta atgtaatcct
    11941 aacttcagag tacgaaccaa tactcccaag agagggtaag gatttcaaac taaccatagt
    12001 tccaaatatt cagagtaaag tagagcagaa gcttgaggaa ggtgttaaaa aaactagatc
    12061 taaaagtggt acagcaattc ttatggatcc aaaaaccgga gcaatcatgg cgatggcaaa
    12121 ctatccttcg tacaatccta atgaatactg gagagtatcc gagccatgga tattaaaaaa
    12181 cagagcaata tctgatgtct acgaatatgg ttctgttcaa aagccaatta ccatagcaat
    12241 agcccttgag agtggtgcaa ttgacaaaga tttcaaatgt gtagataaaa ctggttcgct
    12301 tgatctctac aaagcaacaa aatatccgga tttaaaaggt agatttatat atacttggaa
    12361 caaaaaagct gatggaacac tagacatctc tggtatatta caaaattcaa acaacccatg
    12421 cgttgcacaa atagcactta aaacaaactt tgcggacttt tactccaagc ttaaagattt
    12481 tggaatcgga gaattcattg gagtagggct acaagaagaa accacgagtt accttagacc
    12541 ttacgagcag tggacaaagc ttgatttaat aactgcatca ttcggacaag gaatctccgc
    12601 aacatcactt caggttatat ctgcacttag tacacttgcc aatgatggta agcgcatgag
    12661 accatatatt atttcagaga ttacagacaa caaagaaaca atagttattg agccaaaggt
    12721 aatctcagaa ccagtttcta aagatacagc aaattttgta actaaagcac tacaggaggc
    12781 agtccaaaag ggtttacttg gaggtttagt atcacctgat cttaaaaaat acgacatagc
    12841 tgcaaaaaca ggaactgcac aaattgctaa atccgattct cttggatatt ccaaatacat
    12901 taccaatgca acaacagtag gatatgcacc aacagattct ccaaagttaa tacttcttgt
    12961 taagctcgaa gaaccagaaa ttagtgagtt tgctgcatat acttctaact tagtttggag
    13021 agatattttc cttgcaattg ttaatgatat ggaaatacag aaaaggaagt gacataatat
    13081 atcaattagt taaccacaca acatatgatt gacatattaa aagaaggatt gaatgcgcta
    13141 ttcatctcag ttatagcttc actaatcttt gcgccaatca tgattaccat cctgtacaaa
    13201 ttcaaccagg ttagtggtat taaaaaatcc aaaataggag gtggcgatgg tgacaatacg
    13261 ctcttcatga gaattatgaa tgtaaccaaa acaaatggaa ctcctaacat gggaggcatt
    13321 ctaatatgga tactcgttcc aattctttgc tacttcttag tacctcttac accggtagtt
    13381 aaaatctttc ttttaggatt tctcctttac ggtctttggg gatttataga tgtcgtaatt
    13441 tttaccaacg gatttaaaaa taatgcaaga atgagagctt tccaagaaac atttgaatgg
    13501 aggttgggaa agcttgcatt cgcaacagta atgaacatta tcattatgtt cctcttctac
    13561 agaacaggtg ctttcgaatc catatccttt ttaaatattg caaccataac tgtaacacct
    13621 ttgatagtca tactcactgg tggactctgt cagttagcaa tttactcagc agaactaacc
    13681 gatggattag atggtttaat gattggaatc ttttcaatca taaatatgtc catgattaca
    13741 ctccttgtta tacaaggaaa aaacgaattt atcccaatac tacttatcct tcttggtgtt
    13801 caactcatag acctttactt caatatccct ccagcaagat tctggaatgg tggaccagga
    13861 gcaatgcctc ttggatttac aatgttcttt gtcgcactag ttacagataa tcttttacct
    13921 tattttgcca tgtcctcaat aacatgggga attatggctt ccagtgcaat acaaataatt
    13981 agtatgaagt tcttcaaaag aagagtcttt agaatagctc caatacacca tcatttccaa
    14041 gcgaagggtt ggccacaata caaagtaaca atgagattct ggctatttac agccattaca
    14101 ggaataattg gaatatacct aggaatactt taatcgcact ccacaatacc aaaattacat
    14161 gagcaagctt gtcctgcttc agattgaagc acatctccat taccataaag cttttccttg
    14221 taaacacaaa cacccaaatc aaatatctgt cctagatcgt tttgaaaata taatttaaaa
    14281 tcatctgtta aaaacaaatc ttcttttgtg agtacaaact ttccgtcaat tttacaaacc
    14341 ttgctgtaaa aagatgggtc tctgtttgtt agtacagtca atgttaaatc attctctgaa
    14401 tatttgtaaa atcctacctg aacaggtgat tcctgctctt gcccacacat agcttctctc
    14461 tcaataacaa cagttagaac tccagaagta tatccaacat caacagcctt gatattgtct
    14521 gtatctaact cttctttatc taaatagttt aaaaaactac tgaaatattt taaaccagca
    14581 gaagagtaat tctctaaaga gattaacgtg ctgtcactat tacactggac atttccactc
    14641 ttagagcagt aacaatctgt tcttccatta tagtttggaa ttaactgtcc agaaagatag
    14701 atttcttcat cattggcaca tgtcccaaag cctaagctct ttctcatcga tattgacaat
    14761 gcaataacgg aagatactac caaaaatata gtaataacta ccaaccaatt attttgtttt
    14821 aactttccca tgacggacat attttaccac attacaagct tcaaattaca atttgtgtgt
    14881 cttgtagtaa acagagagga aatatatgtt aaaatccatt tatgcatgac tcacacatac
    14941 atttgaacct ttgtcctctt caggaaaata ttgaacaggt tataaatgac ttccaaaaag
    15001 agaacggaaa gcatattctc actcaaagca ctgacctttt ggattacaaa gaaaatttag
    15061 atatatctaa aagatatcca gatattgttc aacttgcact tggattacat ccgaccgttt
    15121 ttgaagagta cacagtccaa agaaatatat ttaaagatat acagaagaaa tcgcttaaaa
    15181 tgattgcaga ttttgaagat atattcgaaa aaaacatcct agctatatct gctgtaggag
    15241 aaacaggatt agattattac caatttaata acgaccaaac agtagatgaa gatattaaag
    15301 aagagctctg cgaaatacaa aaaatgtctt ttactaaaca tatacaattg gccctgaaac
    15361 ataacctacc tatgagtatt catgcccgag atatgaatgg cagtactgag tgtcttgaag
    15421 atgttttaag gatagtagca caggaaggca gaggacaact aaaaggaagc ttccatagct
    15481 atactggcaa tcttgacttc ttaacggata ttctggatat gggattttac attggtttca
    15541 atggaatcat tacatacaaa agtggagaga atgttagaga catcctaaaa gaagttccgc
    15601 ttgagagaat tctttttgaa acagacggac cattcctacc tcctcaatct gtaaggaaag
    15661 ataaaaaatt aaaagagaaa tatgcacaac cgaaagatat taaagaaata atacagacgg
    15721 cttgtgaagt taagaatata acatacgaaa agatggaaag agttacagac gagaactatg
    15781 aaagattatt tgttctctag gataactaca tactcaccgt gaggattctc tttgaagtca
    15841 tcagtctcta tctccttgct aacttcagag atatttccat agaaaaacat ctcacgcatt
    15901 ttagatatgt ctttagcaat accaactttt ttctcaccaa aaacattttt tagcatatcc
    15961 aacaacttga ttaatctctg tggagattcg taaaaaatta aagatccttc aatatctaca
    16021 tattcttgta aaagcttctt tcttctaaca tttgacttag gaagaaatcc aagaaatgta
    16081 aacttgtctg taacaagccc acttatggaa agtgctgaag ttaatgcact aggtccaggg
    16141 attgaaacaa catcgtactt cctctctcta agttctctaa ttagtttgaa acctggatca
    16201 gatatgagag gagtaccaca atctgagata agtgctaagt ccaaacccat atctaatttc
    16261 tcaagtatct ttcccaacat cttctcatgg ttttgatctc tgtaagagat tagttgtgtt
    16321 ttaatatcat acctattaaa tagtttgtat gattcccttg tatcttctgc aagtacaaag
    16381 gttacttttt tgagagtatc tatagctcgg aatgtaatat cttccaaatt tccaattgga
    16441 gtagctacta catacaactt tcctttattc atttgatatc atctgattta ctttatctag
    16501 gtcaattata ctactattca tcccttcctt tatatcctcc gaggatatgc tatcttcaac
    16561 tttaaaaggt cctacatatg ttcttcgtaa ttcaatagca gttgcatatg taccaagttt
    16621 aattcctaaa tcgttaatta aagtcctaat atatgttcca gtagagcaaa caatcctgaa
    16681 ctttactaat ggccagttgt aacctactat ctcaaattca ccaacagaga tatcttttgg
    16741 cattaattcg aactctttcc cttctcttgc taaatcgtat gctctgactc cagagacttt
    16801 ctttgcagag tatcttggag gcaattggga gatatttccg ataaaatttt ctttaataat
    16861 tttctcaaca acacctcttt ctaaaggagt aggtttgttt tcttttttga gtatttcacc
    16921 tgttccatct tgtgtatctg tcatatatcc aaattctgca gttacaagat actctttttc
    16981 aaattcatgg attttgttca ttagttttgt agctttacct aagagtattg gtagtgctcc
    17041 agtagcaaat ggatctagtg ttccagcatg cccaattttt tgtcctcttg gaagaacttt
    17101 ttttaatttg ttaataactg tgtaagaggt tataccttcc tgcttgtcta ttacaagaat
    17161 tccatctatc atatacatat attctaagtt agttaaacaa tgatatcaca atgttaaatg
    17221 gtattacttt gggaataata attgtaatta caatttttgt attccttttt attatctcct
    17281 tacttacaaa cattctatta gctccagtga ttagaacacc aaaaaaggta atacaggaga
    17341 ttttagaggt tatgaatctt actaaggaag atacttttgt agattttggg tgtggagatg
    17401 gtagattagt tttgggtgcg tatgagcaag caaagtgtaa gtgtattggt tatgatattt
    17461 ctccaattat gttaatgctt gctaagacag caagagtggt aaggtatcct ctttcaaagg
    17521 atattatttt tgaggcacag gatatatttc aggtagatat atctaatgtt acaaagatat
    17581 attgtttctt agatgaaaag agtatgaata ttttaaatga aaagctaagt aattttgtaa
    17641 agggtggggg agaggtatat agctatatgt ataatataaa aggattaaag aatgaaaaga
    17701 agataatact aagtaatggt aatacattgt atatgtatag aggtgtttag ggatcactaa
    17761 gatatatatt taaatatata aggtagtgat ccctatggat ctagagtaac attgaggcct
    17821 gaaggctgag tgcaaaactc agaagggcaa tttccccaac attgattaca acagtctcct
    17881 ttggtagatg aaaaagatct ccaaaaccca acagtaaagc aattgcgatt ataattgttc
    17941 gagcaatagc taatttctct gcagggtata gagattcagg gaggttaata accgcggcaa
    18001 ctatggccaa taccatcaag atattgaaaa aaatctgtct tttcccatag tttgagccat
    18061 gttccctcaa ctccacgaca atcaatacaa cagcaaacac gagcaacgga tttactaaaa
    18121 tctcagctat catttttctt ctcctttttt attttcttta tgaattttta ttttgtctta
    18181 ccgaatatac cttccaccta tgtatgaaaa acatatttgg tagacaaaat aattacatta
    18241 gaaaatatct agatcctttt taaagagcag tatctatatg aattaaatca atatagatgt
    18301 atatattata ctataggaca gcttttaatt caattctcca ttaaacaagg tataatatcc
    18361 atatgccaat atacgaacca ttagcatcaa aatataggcc aaaaacccta gatgatttca
    18421 ttggtcaaca acaccttgta ggggaaaatg ggccaattag aaaatttctc aaaacaggaa
    18481 taatcccctc catgatattc tggggaccac caggaacagg aaaaactaca ctagcataca
    18541 taatatccct taacctctac tacgacttcc acaaattaca agcagtaagc gcaggaaaag
    18601 acgctctaag gaaaataatt aaaattgcac ttaacaacca acaatatggt aaaaagacaa
    18661 tcctctttct agatgaaata cacagatgga acaaagcaca acaagacgcg ctcctgccat
    18721 atgtagaaaa aggagtaatc atattaatag gtgctacaac agaaaacccc tcattcacag
    18781 tcataagtgc gttactctct aggacaaaag tctttgtttt caaccaacaa acagaagaag
    18841 acatatacaa attactaaaa accatactca aaaaggaata cccaaatatc aaagtaaaga
    18901 agaaagtttt gggaatgatg agcaacctct ccaatggaga catacgctct gcaatgaata
    18961 tccttgaaat ggctacaacc ttagtacaag aagaaaaggt ctctgagatt acagaagaaa
    19021 tacttaaaaa cacaattcag aaaccagttt actatgacaa agacggagaa gaacacttca
    19081 acatcatctc tgcaatacac aaatccatga gatcatccaa tccagatgct gcagtgtact
    19141 gggtaggaag aatgctagca ggtggagaag acccactgta tgtagcaaga agattactta
    19201 ggtttgcaag cgaggatgta ggtaacgctg acccacaagc actcatcctt gcgaactcgg
    19261 tatatgaggc atgtcagaaa cttggtatgc cagagtgtca gatattcctt acacagctcg
    19321 ccatatacct ctcaaagtcc cctaaagaca ataccgcata tgttgtagag aatatggtga
    19381 agaaggatat tgaagagtat ggtaatcttc cagtaccgtt aaatatacga aatgcagaga
    19441 ctaaactaat gaaagatatc ggctatggta aaggctatca gtatgatcat gacttaccaa
    19501 acaaaaaatc tgaacaacag tgcctaccag acagattaaa aagcaggaaa tatattaagg
    19561 ataactagac aattctaaat tcaatcaaag tagtattcct tattcttcgc tcaataattc
    19621 ccattatact caacaagagc gcctgaatat ttctttcttc tggagaatca cctccagaca
    19681 tacctctgaa gctattacca ccaagattaa aaattaatcg cttagattgt attcctctca
    19741 aatcaaaatt ttcaatattt tttttgaaaa cagtttctgt atttacagat agatcaagaa
    19801 tatctgaact acttactgga tcaacatcac acagagcctc tcgtaaaatc tgagcctcat
    19861 attcatttaa aataccatta caatatcctg gatttgcctt ttctgtattc tcaactaatc
    19921 gatatcttga attgggaaca atatcggtat cgaaaaatat ctcttcgaaa gatttaagtt
    19981 ttccattaaa atcaattact ccgttttcca ttttttctat gcccgtaatc atattattcc
    20041 tctttctcag atccaatttt actccttatt atatcgctca ctagtaaaaa gactggtgca
    20101 aaagctaact tcctttcgcc ctggaattta agatatatat gccttggatg gttattcagt
    20161 ccaatattaa ccattttgta accatcataa ttttcataat catagacacg agcatataaa
    20221 taattacaac cccacccaat acctaaatct aatgaagtaa cgggatattt aataccatca
    20281 cgacttgact ttttcccttt atactttggc aaaatatggc taaaagatat cgacagatca
    20341 actttagtac cttcctcggg aatatctaaa cccaactcgt catacttttc tcccagaaga
    20401 gacttcagat tctgtgcatc ctcatcggac attggacttt caaggctttc ctcagctatt
    20461 attaatctcc ctatctcgtt aagttcaagg atgtctttta gctccttgat tccttcgaaa
    20521 gggtctcttt ctctttctct ttcggattta cagcctgcgc tcatgaccca taattataca
    20581 ctatccacct tgttctacaa aacatttaca tatataatta ttccgtcatg acatccataa
    20641 aaacagacac agaaataatt gagcgcatac ttaacaaagg agtcgaacaa atactccctt
    20701 ctaaagacac cctcaaagaa aaattactct ctggacaaag attaaatata taccaaggat
    20761 ttgacccaac tgcagaaaca ctacacatcg gacactctgt tggtatgaga aaattagaag
    20821 actttaggaa gcttggtcac catgtcatat tcctcattgg agactttact gcaagaatag
    20881 gggacccttc tgacaaaatg gctacaagaa aaatgctaac agaggaagag gtaaaaaaga
    20941 atatggagaa atatgtagaa caagcatcac acatcataga tatgaacaac agtgagaatc
    21001 cggttgagat aatgtacaac agcaagtggc acaacgaatt aaaatttgga gaaatcataa
    21061 atctagcaag tgaatttaca gtacaacaga tgcttaagag aagtatgttc caaaagagac
    21121 tcgaagaaga caaaccaatt catctaaatg aattcctcta cccactaatg caaggatatg
    21181 attctgtaat gatggacatc gatatcgaag taggaggcaa tgaccaactc ttcaatatgc
    21241 ttgcaggcag agatatggta cttaaccatc ttaaaaaaga gaaaattgtt ttagcaggta
    21301 aacttcttga atcaaatgat ggtgtaaaga tgggcaaatc catgaacaac atgatacgga
    21361 tggacgacaa gccagaagat atctttggaa aagtaatggc atttgcagac gacctcattg
    21421 taattggctt cgaaattctt acctctgcat caatgcaaga ggtagaaacc tacaagaaca
    21481 gactagatgc agatgagaac ccaataaacc ttaaaaagga attagcatac agagtaacat
    21541 ctgaaattac atcagaagaa gatgcaaaaa aagcagaaac atactttgaa aacatttacc
    21601 aaaacaaaag ggatgacgag aatataccag agaaagaaat aaccaaggaa tctataccct
    21661 tagtagattt actccttgaa tgtggtctaa caaaaagcaa atcacaagca agaagactaa
    21721 taaaacaggg tgctgttaaa atcgacaatg agaaacagag tgattggaat ttagtaatat
    21781 cactctcaca aaaaaataca ttcacactta aggtaggtcg ccaactatgt agaatcctct
    21841 acaaaaaata actgggggaa attaaatatt taaaatatat acccccaata caagaatggg
    21901 ggttttttaa ttatgaacaa aatagaaaat ctaaaagcag aaatactaaa caactcacct
    21961 gagaattaca aatctcttgt aaatgatgcc ataaagtttg cacaagaggc tcacgaagga
    22021 gaaagtagaa aatctgggga agacttcata atccatcctt taaatacagc ccttaccgtt
    22081 gcaaaaattg gcctagatac cgacaccata atcgcctctc tgcttcacag tcttgcacac
    22141 aaagaagata agatgaatga gataaaagag attttcggag aagatgtcta caacctaata
    22201 acacaaacaa gaaaaatcca cgatgctaca aaaaacagca gaactcacga cgaagtaatt
    22261 accaaataca ttcttaacag atccaaagat atccgaccaa tcataattaa acttgcaagt
    22321 gctcttcata atgttcaaac catagaacat ctgccagatg aagagctaaa agatacactt
    22381 agaaaaatct tcaatattta tgcaaattta gcagagtatt tgaattttaa taccataaaa
    22441 aaagagttag aggaaaatgc atttaaagct taccaaccag tagaatatga aagcataaca
    22501 gaaaaaatgg aaagcttgaa tattaacgat gaactcttaa acagatatcg agaggttatt
    22561 acaaaggaat taacagatat acctgtaaaa gtggagggaa gaatcaaagg gaaatacagt
    22621 atatataaca agttaaaaaa atatgaaaag gaatggaaag atccaaatat cactagctta
    22681 acagacatta ttgctttccg agtcataacc caaaatatgg acgattgttt tctcgcatta
    22741 gaaaaaataa tggataatgc agagctaaac accgaactct tcaaagacta cattagtaat
    22801 ccaaaaccaa atggatacca agcaatacac tccatcgtca aatttccaga gatctcaaat
    22861 cttgagatag aggttcaaat tcttactgaa gttatgcatt atacaaacac atatggtaga
    22921 gcatcacaca ttgcgtacaa agcaagccaa agtagatttg cacgaccaac agatgtgtat
    22981 tcatgggtag aggatgtaca taacaaaatt gaaagtcata taagtaaaag aaatcttaaa
    23041 agatcaatac caatcgaatg caatatcttc gagaacgaag tatatgcatt cactcctaaa
    23101 ggagaaataa tccccctaac gaaaggcgat actgcactag atttcgcctt taaagtacac
    23161 acccaaattg gtaacagtgc cataggagca aagataaatg gaatagcagg taaactggat
    23221 accgtactta aaacagatga tgtagtagaa atcaaaacac agaaagacaa aaaatggcaa
    23281 aatgaaagtg cactaaagat agttaattca tcttctagta gaacaaaaat tcttcagggt
    23341 atctgtaaga aaaataaaaa ttaatgtaga attaaaaaca tgaagaaaag tactactaaa
    23401 aaaatacaga acattgggaa tgtattaatt gccattattc ttcttggtgg aatactcatt
    23461 ccaataatac tttccttgtt cctttcataa tatgccttta acagcagatt cacagattac
    23521 agaaatcaaa ggaatcggag agagtctagc aacaaaattc aatcatttaa atatcaatac
    23581 cgttaaggat ttacttttcc acgtaccaat taaataccaa gattcatcac acttgcttcc
    23641 aattgaggag ttcctccttg aaggagaggg cactttcctt gcagagattg tagatattaa
    23701 aacttttaga acaagaagta gaaaaacaat taccattgtc aaagtaaagg acgatacaaa
    23761 gaaaattaat ctcacattct ttaaccaacc atatctttca aaagcactaa ttaaaaacga
    23821 aatatatctt ttcgatggga aagtaacaat taaaggtagc aaaagagaca tttacaatcc
    23881 caaatacgaa atcttcaaag gtgaaaaaga gaaacaaaca aatctaggga aacttgcccc
    23941 catatatcct gagaccgaag gattaacctc caacatgatt agaaaaattc ttaaaacagt
    24001 aaaaacaaat atccctgaaa taatctcaga tccaattact tcttacacag agacatctct
    24061 tcaggaggca ctttctaaaa tccactttcc aaaagataca gaggacatat taattgcgag
    24121 aaaaagattg gcttttgacg agatgttgcg aatagctttt aagattgaac aggagataga
    24181 agaaagaagt acacaagtag cacttccaat gaaaacaaat tctaaaatat taaatgaatt
    24241 cataaaatcc cttccataca aattaacaga tgaccaaaac aaatccttag aggtaattct
    24301 gcaagagtta aatagcaaca taccaatgaa tagactactc aatggagatg taggaagtgg
    24361 aaaaactgtt gtagctgcag gtgcaatact taatacaatt aaaaatggct tctcaagtat
    24421 tctccttgct ccaacaacag tactagccaa acaacactac gatacattta aggaactttt
    24481 caaaggcttg cacattccaa tagaactatg catatcttct gaccaaaata ttagtgatgc
    24541 aaacaacaaa ctcataattg ctacacactc catcctttac aacaaagaac tccctacaga
    24601 tttgaatctc gtaatcatag atgaacaaca caggtttgga gtagaacaaa gaaaatattt
    24661 cctaaaaaaa ttaccacaca caccacatca tctaacaatg acagcaactc caatcccaag
    24721 aagtttaaca gagatcttct ttggaaatat ggatgtatcg gagattaggg agaagcctac
    24781 aaacagaaaa gaaattaaat cgtactatac accctttgca aaaagagatg attgcttcaa
    24841 ttggatagca gataaaatag aaaccagtga gaagaaggaa caagctttca taatatatcc
    24901 actcatagag gagacagaag tttctgttgc caaaaccgtc ctagcaagtt ttgaagaatt
    24961 gaaaaagaaa tattttaaag acataccaat aatatatctg catggaagaa tgaaggatag
    25021 tgaaaaaaac aaaatcctca atgatttcaa aaagaaaaaa tacaatgtct tggtgtccac
    25081 atcggttata gaggttggta tagacatcca agatgctacc attatggtaa ttgagaatgc
    25141 agagagattt ggtctagcac aactccatca gttacgagga agagttggaa gaagcgacaa
    25201 gcaatcatac tgctacgcca tcccaggaat agaagtagcg aaagattcca aggcagaaga
    25261 gagactcaaa tattttagta aacatagttc tggatttgat gtagcagaat atgatctgca
    25321 gagtagaggt cctggagagg tatatggtat agcacaatct ggaatcccta acttcaaggt
    25381 tgcttcgata catgatttgg atacactaaa gaaagcaaga aatgttgcaa aaagattgtt
    25441 aaaaagtaat aatcaaatcg agaatatttt agagaacata tttagatgaa aatacatatt
    25501 gtattagata atattagatc tgtatttaat gtaggtagca tatttagatc tgcagacgga
    25561 gcaggcagtg ttgagaaaat atatctctgt ggtatgacaa caccaataga taacccaaag
    25621 cttgagaaaa cagccctagg agctacagaa atggtagatt ctgagcacta cgatacaacc
    25681 caagaagcac tagatgaatt aagagaaaaa aatattccaa tctactcagt agaattaaca
    25741 gatgatgctc aaaattttca aaaagtagaa tatcccgaag aattagcact agtctttggt
    25801 catgagaaga acggagtaag cgcagatata cttcaagaga gtgacaaaaa aatatacata
    25861 cccatgaggg gaaagaaaga gtctctcaat gttgcaaatt gtgcaagtat aatcctgtat
    25921 gagattacaa gaaaaagtta actttttctg ctaaaatgtg gtaatgaaac tttc
//
LOCUS       DAOQWP010000011        25243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_2291_length_25243_cov_8.340003, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000011 DAOQWP010000000
VERSION     DAOQWP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 25243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25243
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2291_length_25243_cov_8.340003"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="PKI16_02055"
     rRNA            complement(<1..1123)
                     /locus_tag="PKI16_02055"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1420..3243)
                     /locus_tag="PKI16_02060"
     CDS             complement(1420..3243)
                     /locus_tag="PKI16_02060"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HNR53237.1"
                     /translation="MGITFHHIATKFEELEKISSRNDMSVIISELFLGIDTEEMQILS
                     YMLQGRVAPLFVVSEFNLSEKSIFKLLGNILSSKGAKYDLEGRRIKLGDVGEVVKEVT
                     EQLGYESKGLSLSEVYEYMWEIIQSSGTGSAEKKANIVGELFKKMSPIESKYLARIIC
                     GELRLGINAKSVLDAFSNIMQGDKGKRELLDRVYGINPDLGYVSTILESENNVSTTIS
                     PGVPILPRLVERVGSFEEVFERFEGEFLVQPKYDGLRCQIHKYSGVDVKEDGLVWRKH
                     LKKDNAVRLFEENGSVKEVRLFTRNLEDVTEMFPEIVEEAMSIKAESFILDSEILGWN
                     HEKNEFLSYQETMQRRRKYGIGEKAKDIPVKAMVFDVLYLNGKDISELDTDVRVKVIK
                     EIIPDTARGIGPSPTEGVEDMKRLKEIFYRRVEEGLEGIIVKQKSGRYLPGVRNYEWI
                     KLKKSMEKGLVDTIDLVAVGYYHGSGKRGALGVGAVLGAIYNEEDNTYEAVCKVGTGF
                     SDELLLNISAEFKEISLIKKPINVLVEETLLPDVWIDPKIVFSVESDEITRNLNADKG
                     IAGGLSLRFPRLIEWGRDKTAEEATSLEELMRLFSLQKKNK"
     gene            complement(3248..3604)
                     /locus_tag="PKI16_02065"
     CDS             complement(3248..3604)
                     /locus_tag="PKI16_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNR53238.1"
                     /translation="MKKDQAVLNTILGLSGFVIGAFLITNLVHNLSKKTNTSKKNVVG
                     KTESKNIDEFLNERQKKAVEILSTKGRVTVKELVIHFPKVSTRTLRRDMDALARRGDV
                     SQEGSTKSTYYRYLLK"
     gene            complement(3641..6322)
                     /gene="polA"
                     /locus_tag="PKI16_02070"
     CDS             complement(3641..6322)
                     /gene="polA"
                     /locus_tag="PKI16_02070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNR53239.1"
                     /translation="MNLIKTSKDRFLAIDANAIVHRAFHAYPGNLQTDDGVQVNAVYG
                     FTVMLLSALKSFDPKYVLCAFDTSKPTFRHEEYIDYKATRKPIDQSLIDQFPLVEEVL
                     KAFNIPIIKKEGFEADDILGTIAKMVDSGKWKDQNLELYILSGDRDLFQLVRGDVKVC
                     LPSGNFSNLIEYDRENVFKTLGVYPEQVVDYKAIVGDASDNIPGVKGVGDKTVVELLK
                     EYGDFETIYKNLPKLKPRLQVLLGEGIEQAELSRKLAKIDQDMDLEIFLESCLMQDFD
                     RKNVLDLFRRFKFKSLVARLDDIFGKDSSGYSPQLNIFNESAGNVEWASEEEIKSLSK
                     NASSIVCCFLSKEETTTDKELILTRFVDKMRVKTDRAYENGSYTLRANCETVFCNFEN
                     LVSHKETTIDVDLAKSFDISLFSHIINSEKRDQSLKDLAFDYVGKVFREKISPLEIST
                     VLDTVEEIKNAQINKANEIELYEYTLESIKKYLLVRSEYLLNVLRKIEMPVALVLSKM
                     EEKGIKVDIKYLEELNEELANKLEKTKEEIFATVGHEFNINSPKQLSDVLYNELGLPS
                     KNGFSTREDILVSLSGAHPCVEKLLEFRELSKIYGTYTSPMLAMAKDSDDQSIHTDFK
                     QTGTTSGRFSSINPNMQNLPAQGEWSEKLRKAFVAREGFKLVGMDYAQIELRIMADMS
                     RDNLLIKDFNDGLDIHTATAARILEKEIEDVTKKERSIGKTVNFAILFGQTPFGLSSL
                     LKIDSKVALEYIQNYFEHYLGVEEYIRTLEREAYKKGYVQTMFGTTRMIGGIRSKNIR
                     MNKAAKREAVNMPIQGSEADIMKLGMVKLDELISKEFNDDAYILLQVHDELVFEVVKG
                     RVEEFEKKAVDILKNIVSLEVPLDVHVSHGDNLAELK"
     gene            complement(6341..8197)
                     /gene="ftsH"
                     /locus_tag="PKI16_02075"
     CDS             complement(6341..8197)
                     /gene="ftsH"
                     /locus_tag="PKI16_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNR53240.1"
                     /translation="MDNKKNIKKEVKIDIRKLPIRRGLGIPNIIAMVVISLALTGAII
                     FFRGLGTRGEEVSVSKIVTDISEEKYDKIILKDGIVTLEMKGEGNIKKSEYALLPGGT
                     DFYQILSDSGLNIKDLENDFYQPAIGITFGDIVTFVLLGAGLILVFIMIKNMQASGGK
                     IMDFGESKARLLFGKKTGITFEDVAGIEDVKEELTEIVDFLKQPKKYLAAGARIPKGV
                     LLAGAPGTGKTLLAKAVAGEAGVPFFHTSGSEFEEMLVGAGASRVRDLFKKARSASPC
                     IIFIDEIDAVAKKRGTVLHSGAGEQTLNQILVEMDGLEERQNVIVLAATNRPDVLDPA
                     LLRPGRFDRTVVVNMPDFKGRRAILDVHAKNKVFEAGVDLDILAKKTVGYSGADLENL
                     LNESAIMAARENRKKISQEDIMEAYLKVKLGRKKTGDKEAEDIRKTAYHEAGHAIVAK
                     FTEGSDPVEQVSVISRGLTGGITVYLPEKDTKFLYKKQMLAWLRTGVAGRVAEELFIG
                     EISTGASNDIEQITGVAREMVMQYGMSEKLGMIKYGDMEETKHLGYTYGGGREYSEKT
                     AELIDQEVKRFVDEAYEYSKKLLQEKKEYVEKLVNILVEKEVVVREEFDALF"
     gene            complement(8863..8936)
                     /locus_tag="PKI16_02080"
     tRNA            complement(8863..8936)
                     /locus_tag="PKI16_02080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8900..8902),aa:Met,seq:cat)
     gene            complement(8980..9540)
                     /gene="efp"
                     /locus_tag="PKI16_02085"
     CDS             complement(8980..9540)
                     /gene="efp"
                     /locus_tag="PKI16_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNR53241.1"
                     /translation="MPLTNQPVKGMYLLEDGKFYFLIDRQLKTQGRQGGLIILKMRAL
                     ESGYVTSKTIKAGTKVEYIEPETKEMQYLYSDNTSVYLMDSETYETITIPKETIGEYI
                     NFLKEGDTVLIMVYEGKVLTIKENPTVVLAVVDTSDAVKGNTANSATKEAVLETGYKI
                     QVPMFIKKGDVVKVNTDSGTYSGKAN"
     gene            9619..10158
                     /gene="hpt"
                     /locus_tag="PKI16_02090"
     CDS             9619..10158
                     /gene="hpt"
                     /locus_tag="PKI16_02090"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNR53242.1"
                     /translation="MTNPKDNTVQREILISSEQIKERVAELVIEIKEDVDVDNLVLVG
                     VLNGASFFTTDLAKEFNNPKVEIDFMSVSSYGNKTESSGEPTIHCDLKNSIMGKDVVI
                     VEDIVDTGYSMAILLEILRARRPKSLRVCALLSKPDRREVDVPIDYIGFTIPNHWIEG
                     YGLDTNEKCRCLKNITRRV"
     gene            complement(10162..10236)
                     /locus_tag="PKI16_02095"
     tRNA            complement(10162..10236)
                     /locus_tag="PKI16_02095"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10202..10204),aa:Met,seq:cat)
     gene            complement(10278..10724)
                     /locus_tag="PKI16_02100"
     CDS             complement(10278..10724)
                     /locus_tag="PKI16_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HNR53243.1"
                     /translation="MDRVIYRQPDKRVKQEKEIVIMSLFNSSVAKVVLLGISVFLVYS
                     IFHSVSITVQKVEILNKARVEVEDLRLRNLELASLLERMQTVEYLEVEARDRLNFTGK
                     KEIVFVIPENMYPVAREYVDSLLSENEVDSSEYGLEVWKSFLSSGI"
     gene            10858..11745
                     /locus_tag="PKI16_02105"
     CDS             10858..11745
                     /locus_tag="PKI16_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR53244.1"
                     /translation="MGAINNPNFEEKRVKFIADLQSKGRSEATVIAYSKDIEQLLNFF
                     SKEGISSIEDTTIEDLEKYKQNLQDNNYTPKSISRKINSTRTFYRYLLDNSLIQDNPS
                     EKLAHPKFETKPPRVLTEMEYRALRDVSRVDVRLYSIVEILLQTGIRIGELAALTLDD
                     IRKSNDGRIDYLYIKAAGSHPQRKVPLNRSAKKAIDEYLELRPETEDDTLFVTKNGRP
                     LLVRNIRTAIDKSFEKAGIKNAKVNDLRNTFIAHHLANGVSLVVVSKLVGHKRLSTTE
                     KYLNLVKMQKEDQKSLKEL"
     gene            complement(11754..12014)
                     /locus_tag="PKI16_02110"
     CDS             complement(11754..12014)
                     /locus_tag="PKI16_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNR53245.1"
                     /translation="MSEKESIGEREIMIGGVYKHFKGDYYQVLTVGKDSETEKDMVVY
                     TSLYYKEDKGTRVWIRSLEDFMGTKELEDGTIVNRFEFVSER"
     gene            complement(12041..12117)
                     /locus_tag="PKI16_02115"
     tRNA            complement(12041..12117)
                     /locus_tag="PKI16_02115"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12081..12083),aa:Ile,seq:tat)
     gene            complement(12166..14679)
                     /locus_tag="PKI16_02120"
     CDS             complement(12166..14679)
                     /locus_tag="PKI16_02120"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HNR53246.1"
                     /translation="MDIYAQTFGPTLEDNTEIETGTSTLYPKDTYGDMYGPKEINIPW
                     GWILLVLILIGIGVGVFLYVRKKLLAKDRNEKAGEGVLMEVQVPSGNETEIGVAEKMF
                     ANLYGIGGKGSGIAEHFSVNNCVTFEVIGVPGEIRFYVHCPRKISDLVEKQILGSYQD
                     AEVRVVTEYNIFAPDTKTACTRLILAEDPYCPLRVASDFTGDPMANVLSTLSKMSENE
                     GALVQLAVSPAGKEWQKNGEKFVQKIKNNNSDPEKKKIGISDEKLEGISKKVAKPGLI
                     AEIRIVTSAPSEDIAKMHLDNIVGAFDQYSNPGINRLEKKKVSEKEQKKFLYDVVFRR
                     TPETENSILNVEELAALYHFPNKDTNTPNIKWLLARELIADNSISSDIQSKDTIWIGN
                     NIFRGKKKVICFERDDRRRHAYILGQTGTGKSWLMTRMIIQDIYNGDGVCFIDPHGQT
                     AEMILERIPPERAEDVIYFDAGDFERPFGLNIMEFYNEQHKHQIVNSFIALLIKLFDP
                     NNQGYAGPAFQQAVRNSMLTAMSKEGSTLIEVVRILQDEDWVTNYWLPLIQDELVKRY
                     WIDQVAKTDKKTKAESLTYFISKFDKFTTNLAIRNIIGQSKSSFDFRDVMDNGKILIV
                     NLSKGKIGEENMSFLGLLLVQKLLAAALSRENVDEKSRKDFFFYADEFQNFTTDEFAS
                     ILSEARKYKLNLTLAHQYIGQLPENIKGAVFGNVGTLMIYRCSAEDANFLETQFDPYI
                     KAGDMVNQGIGHCYVKLLSKGSYPAPFSLNTLYGEEFPNSGFDLPENKEVAEMIRKYS
                     RAKYGRDLEVINEDIRIRSQLDRKEEPVSTPQPFPPMSF"
     gene            complement(14732..15022)
                     /locus_tag="PKI16_02125"
     CDS             complement(14732..15022)
                     /locus_tag="PKI16_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53247.1"
                     /translation="MDNTRIETQPMGIGGVETPVISSEIETEVGAVENSSTTMTEVSP
                     KVDTIPEPIETSVGAEVSIETPKPSAGFQIDPKSTGASKIVLEKLTNTIANL"
     gene            complement(15041..15484)
                     /gene="smpB"
                     /locus_tag="PKI16_02130"
     CDS             complement(15041..15484)
                     /gene="smpB"
                     /locus_tag="PKI16_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNR53248.1"
                     /translation="MKIINKKARFDYEILESFEVGIVLLGMEVKSVKSGRVNISDAFV
                     KIIGDELWLVNADITKYQYASDDHYDSKRTRKLLIKRNQLDKLESKMKQGNLTLIPLS
                     IYTVRGYVKVEIGLARGKKYHEKRKAEKERDMDRDLLYESRKFVV"
     gene            complement(15497..17671)
                     /locus_tag="PKI16_02135"
     CDS             complement(15497..17671)
                     /locus_tag="PKI16_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53249.1"
                     /translation="MKITAQQFVSNDKDKNTFVSVFKHYLNLTEKKISKEGDLYALLS
                     ISGSKNLTAERVSKFVWDGLVDGYLYSNAKTTNESLKDSIKEGIHKIKDLIRNDQTLE
                     QEGVNINFVVVAQKKEGLYLGNFGENDIYVYKDNRFINIYDVLKEKKSSTAGIALAQD
                     DILLISTAGLLTEGMATFTGHKNKQEVLESLEKFGEKILNSEGIIYFQNTEEEKDNVK
                     NIEVKADILKDIPKPRIKMPQKEKKKFKIPLNLKPLIEKILQFFKEAVSKIKSVVTSL
                     VGKQHWFKKVSSKISENKVFDRRESMKGMKIDGYKERNIRVSRIRTVVVVVLIIVLLA
                     VGINFTINAKKARERHNIAQESFTKIEKLLDRAEGDMVVDTSAAELSVFQTEKLFEAI
                     PSELNDEDTTKLQELKDRRLNIEDTLFKRKVISDGDGSISTYIDSRLSFGEGSAPSDI
                     EIYTDKTGNSYLLVTDLGLKSVYRVSLYDKKVEKILDTDALIKEPKYVSVGNKGIYVY
                     DAKEGVVKAPFDGSKVKTFVKLSGLGIDDIEEKEITEMIVLTESDNVYLLSRADKVLL
                     KSSFSDGNSYSLSFGYIRNDNFATVNDISSDLSLYFLTSDSGLIRYSYSYFEQKQALS
                     PLTTGGFDGNYGNLTKGYTTGSLDYSLYLFDAQGRRFLKFEKPQEGGGDIRHPGQILL
                     LNQIIYRGTKDGMWSDVKDFVVDSKESAMYVLDGSTIWKIVL"
     gene            complement(17673..17942)
                     /locus_tag="PKI16_02140"
     CDS             complement(17673..17942)
                     /locus_tag="PKI16_02140"
                     /inference="COORDINATES: protein motif:HMM:NF040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53250.1"
                     /translation="MENILEILNNISGTTTDTINLGIISLLKLPLIIIIFGNVLFAVL
                     LLLRIRILSETFETTDNKLIRYLVRFYLFISVIGSIIALLLLLIG"
     gene            complement(17942..18562)
                     /locus_tag="PKI16_02145"
     CDS             complement(17942..18562)
                     /locus_tag="PKI16_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNR53251.1"
                     /translation="MAMKEDKNKIPVKFRNIKKSFMDTISLNNNYSFMSFPKELNFHG
                     KEKGEQVVLIIRSHWIVTVPYFFLAILVFILPFILASLMPTFFSSIALLISLVIISLL
                     VSLGIVVSTFVKWFYTVHIITDQKVVDLDFTSIMNHSMAEAQLERIEDVTHKQLGAIG
                     SIFDVGSVYIQTAGAQNEIEFTNVPRPRDVQDILFDLLELKQKGEI"
     gene            complement(18606..20480)
                     /locus_tag="PKI16_02150"
     CDS             complement(18606..20480)
                     /locus_tag="PKI16_02150"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNR53252.1"
                     /translation="MPKKPQNIIQLFLVVSLGIFCVTLFNFPAYSGEIEDLENQINQT
                     NEELAKKKGILSDIEKKIAEISGSNYSLSQKISLINDEITKLKKSIDSTEADLNKKIA
                     EIEEKQVLLEKKKESIDTFSSDLYIQSRYRMSQFFLSRDSWDGFVEGLFVKKRAITVL
                     RDEVEKINGEFISLAESREALEKQKTELEEQKGDLDKSYALLAAEKAKLQKELNAQIA
                     SRTSISSQINKLTSQLSNLQKTLLYTRQGGTSVDPSQVTAGGSDLGSLSTFTSKAPVG
                     SFGVFSIGAYTHRNGMSQWGARARADAGQTYTQIISAYYPTKRITTGVVNIGGTVKNI
                     MTDIDTTTYGELNFEDDYMLRLREIPDDWTQTGSSSLNLKRMNVLKAQAIVARTYAIN
                     YTSNGSKAICTNENCQVIGSNKKTGAWATAVKETKGMILTDSSKRVFSAQYAAVHGGW
                     VNTIGWDLVDGSGTKDFTNTWDNKSGVSWTYRSWYRRDYNLSTSTSSSTCYRYPWLSG
                     QEMADIVNAASRLTDIGGSGTSDSRIYPIHDRCHSDGNPYSFTEMRNKSSSKFTKVIL
                     AQVSRSSGNTNSIKFVNEDGREITISGSYFKLAYNLRAPGYLSIPQSGFVHIDIQKKL
                     "
     gene            complement(20488..20880)
                     /locus_tag="PKI16_02155"
     CDS             complement(20488..20880)
                     /locus_tag="PKI16_02155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNR53253.1"
                     /translation="MKNDHFKSPDFLKSQGYARNGVKLIFRNERNFRIDIVIAVLVVI
                     AGFLFKISHTDWIAISLVISMVFTAEVLNSAIEALCDTVSQDFKVNIKYAKDVSAGAV
                     LISALVSIITGLIVFLPYIMEVVKNILV"
     gene            complement(20881..21195)
                     /locus_tag="PKI16_02160"
     CDS             complement(20881..21195)
                     /locus_tag="PKI16_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNR53254.1"
                     /translation="MKIFKKLVFLFSLSLANPVFAQEEEPKKIGELFDVLDNVLQYIF
                     PLAGLICVVFVIIGGYMWMSAAGDPSRVKSAQSTLTWAIIGLIVVLLAVAIIEVIVKF
                     IS"
     gene            complement(21498..21854)
                     /locus_tag="PKI16_02165"
     CDS             complement(21498..21854)
                     /locus_tag="PKI16_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HNR53255.1"
                     /translation="MKAPKVLAIASSVFSGLYLASGTYAQTALSWVDRLKGTGGVTQP
                     LESWIGGVINLVVGLAALVCVGILIASGYMYITAAGDETKVEKAGKSLTYAIVGLVIC
                     FIAVILVEFVLKNVLK"
     gene            complement(21924..22226)
                     /locus_tag="PKI16_02170"
     CDS             complement(21924..22226)
                     /locus_tag="PKI16_02170"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53256.1"
                     /translation="MSKIDISGIVSGLLPTFGSDSDIEDLIGTKVADAINFFTGFAAL
                     VAVVLIIVSGYMFITSSGDPDKVDKAGKTLSGTIVGLIIVFIARILVEFVLKVVVK"
     gene            complement(22219..22569)
                     /locus_tag="PKI16_02175"
     CDS             complement(22219..22569)
                     /locus_tag="PKI16_02175"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53257.1"
                     /translation="MRILDTLNMFLVEKVHAQSLGSSLGDQLNKIEDGTVTDESDLVQ
                     TIVSIAVPLGVISVVILVVYAGYLLTSSQGNPDKLKEGKDIITNAIIGFVVVLLSVAI
                     LILISNTLGLGIYE"
     gene            complement(22593..>25243)
                     /locus_tag="PKI16_02180"
     CDS             complement(22593..>25243)
                     /locus_tag="PKI16_02180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNR53258.1"
                     /translation="PTKFLNDLIKHFSRLQDEDVSDEEYIKYAKALPKKTPEEKASFE
                     KYKELSETYRKYSELKIENSKIDFGDLIILTLKLFRERADVLEKYRKQFKYILVDEFQ
                     DTNYTQNVLINILKDGKRHKLTVVGDDDQAIYKFRGAAISNILQFKEKYPEAMEIVLT
                     DNYRSRQEILDAAYALIKHNNPNRLEETEKIDKKLVARGAFEPQEEVVSLIAAKDEYT
                     ETERVVGEILKLTGYGEHAQEGSGAQIFDEKGQAGFLGLSKKGKFKFSDIAILVRANS
                     HAEPFIQELRNKGVPYKLGGARGLYFRPEIQNIVAFLRVLVDYSDEIAMYRLLSMNIW
                     NLTVREYIEINRLAREEKLTLFEELEKLWDVKIGEVEGEIDDVSNPLVLKMLDAKAIA
                     GISNLLEIVDSSLKRIKDGRPILEILYDYVKVSGYLDSFLGEESGENLFAVSNIQKFF
                     ELVKKYEKDNPDSNIYEYVDYLNYCMEVGESPVVDQSELEDFNGVNILTVHGSKGLEF
                     PVVFLVNLVSQRFPSQNRSDAIPIPEELIKETLGENFSESNSHLQEERRLFYVGATRA
                     QEKLYLSAAKYYGDGKSSKKPSIFLNEILDRNVEDEFVGLIKDGGMKIASMDLDTYES
                     IVPKEVKLDVIKNFSYSQLHTYEDCPRKYEYSYVLKVPQKPNSSLSFGTTIHNTLKEL
                     YTALKHSKEGLEGVYAPPTLEDLLGYYEKNWVSTGYESTKQEQLRKEEGRKILKEYYN
                     KMYSEQQKPLKLEESFSVHFGESVFVGKIDRIDMVGEEGEVCIVDYKTGKVKNEANIK
                     KDLQLPLYAVFAQEKLGLKVVSAKYIFVESMEEIEVDISQKRKDLAKEKLLEAIGNIR
                     ERKFKATPGFGCNFCDYNSVCEFAEI"
BASE COUNT         7416 a         5430 c         3751 g         8646 t
ORIGIN      
        1 gacttaaccc aacatctcac gacacgagct gacgacggcc atgcagcacc tgtgtatcac
       61 cttcgaaagc ctcccctatt tctaggggca tgcagataca tttcaaaccc tggtaaggtt
      121 cttcgattgc cgtcgaatta aaccacgcgc tcctccgctt gtgcgggtcc ccgccaattc
      181 atttgagttt tagccttgcg gccgtacttc ccaggcggga tgcttaacgc gttagctgcg
      241 gcacttggta cttccactta gaatccgaag atagaaagtg gtcataccaa acacctagca
      301 tccattgttt acagcgtgga ctacacgggt atctaatccg ttttgctacc cacgctttcg
      361 agccttagcg tcagacaata gccagtgaac cgctttcgcc actgatgttc ttccgaatat
      421 caacacattt taccgctcca ctcggaattc cgttcacccc tctatgtctc aagtctgata
      481 gtttcgaaag ctctccctta gttaagccaa ggtctttaac ttaagactta tcaaaccgcc
      541 tacgcatctc tatacgccca gtaaatccgg ataacgttcg taccctacgc atcaccgatg
      601 cttctggcac gtagttagca ggtacttatt catatggtac agtcatctat tcttcccata
      661 taaaaggagt ttacaatccg aagaccttca tcctccacgc tgtgtcgctg cgtcagggtt
      721 gcccccattg cgcaagattc ctaattgctg cctcccgtag gagtatggac cgtgtctcag
      781 ttccatggtg gctgaccgcg ctctcacgcc agctacccgt cttagccttg gtgagctttt
      841 acctcaccaa caagctgata ggatgcggac tcatctcaaa gcgcctgagc tttacttgtt
      901 caaacattat gaaagaacaa gactatcgag tattaccccg cctttcggcg agctgttcac
      961 gactttgagg cagattatcc acatgttact cacccgtttg ccactattct aagcgaaaaa
     1021 tcatcaaccg aagtttcaga aatttcataa agaatcgttc gacttgcata ccttaggcac
     1081 acagccaacg ttcatcctga gctatgatca aactctcatt gaaaaacgct tgactaacat
     1141 attaccgaag taatatgacc aaccagcatt attgttggat ttaaaatttt tctgactaaa
     1201 tgaattgtgt gaccctcaca ctaattagaa agtctatgtt ttagacttcc tgtcactctt
     1261 caacttttaa ggagctgaga gagataacaa cgaagataag aaacaaacta ttgaaccagg
     1321 atgttcgtta gagctaatct ctaagaggaa tcctgtatct ccttaagtat cccaaactcg
     1381 tgagaggatt ttaacacaac attattccat atgcaagggc tatttattct ttttctgtaa
     1441 agaaaaaagc ctcatcaact cctcaagaga ggtagcctct tcggcagtct tgtcacgacc
     1501 ccactctatc aaccgtggaa accttaaaga aagccctcct gcaatacctt tatcagcatt
     1561 taagttcctc gtaatttcat ctgattcaac agaaaaaaca atctttggat caatccaaac
     1621 atcgggaagg agagtctcct ctaccaatac attaattggc tttttaatca aagatatctc
     1681 tttaaactct gcagatatgt ttaacagtaa ttcatcggaa aaccctgtgc caaccttaca
     1741 tactgcttcg taagtattat cttcttcatt atatatagcc cccagaacag ctcctactcc
     1801 aagtgctcct cttttaccag aaccgtgata atatcctact gccacaagat ctatcgtatc
     1861 aacaagtcct ttctccatag actttttcag ctttatccat tcataatttc gcactccagg
     1921 aagatatcta ccactttttt gcttcacaat tattccttcc aggccttctt ctaccctcct
     1981 ataaaagatc tctttgagcc ttttcatatc ttcaacacct tctgttggag atggacctat
     2041 accccgggcg gtatcgggaa ttatttcctt gattactttt actctgacat cagtatcaag
     2101 ctcggatatg tcttttccgt tcaaatacag cacatcaaaa accattgcct ttacaggaat
     2161 atccttagct ttctccccta ttccatactt tcgtcttctc tgcatggttt cttgatatga
     2221 taagaattcg tttttttcat gattccagcc caatatttca gaatccagta taaaagactc
     2281 cgctttaatt gacattgctt cctccactat ctcagggaac atctcggtaa catcttctaa
     2341 atttctagta aataatctaa cctcttttac agagccattt tcttcaaaaa gtcttactgc
     2401 gttatctttt ttcaaatgtt ttctccaaac caaaccatct tctttaacat caactccact
     2461 atatttatgt atctgacatc gcaacccatc atattttggt tgaacaagga attctccttc
     2521 aaatcgttca aacacttcct caaaagaccc aactctctca actagtcttg gcaagattgg
     2581 aactccaggt gaaattgtag ttgatacatt attttcggat tcaagtatgg tggaaacata
     2641 ccctaaatct ggattaatgc cgtagactcg atctaggagc tctctttttc ccttgtctcc
     2701 ctgcataata tttgagaatg catctaaaac actctttgca ttgataccga gtcttaattc
     2761 accacatatt atccttgcga gatacttact ttcaattgga gacatctttt taaaaagttc
     2821 ccccactata tttgccttct tttcagcaga acctgttcca gagctttgga ttatttccca
     2881 catatattcg taaacttcac tcaaggacag tccttttgat tcatagccca actgttcagt
     2941 tacctctttt acaacctcac ctacatctcc aagcttaatt cttctacctt ccaaatcata
     3001 tttagctcct tttgaagaaa gaatattgcc cagaagcttg aatatgctct tttcagaaag
     3061 attaaattca cttactacaa ataatggtgc aacccttcct tgcaacatat atgacagtat
     3121 ttgcatctct tcagtatcta ttcccaaaaa caactctgaa atgattacag acatgtcgtt
     3181 acgagaagaa atcttctcta attcttcaaa ttttgtcgct atatggtgaa aagttatacc
     3241 catggtgcta ttttaaaaga tatctgtaat acgtagattt tgtagagccc tcctgcgaca
     3301 catctccccg tctagccagc gcgtccatat ctcgccttaa ggttcttgtc gaaactttag
     3361 gaaaatggat aaccaattcc tttactgtaa ccctaccctt tgtcgaaaga atctccacag
     3421 ctttcttctg tctttcattt agaaattcgt caatattttt actctctgtt ttccctacta
     3481 catttttttt actcgtattg gtttttttac tcaagttgtg aacaagattt gttatcaaaa
     3541 acgcacctat tacaaaacct gaaagtccta atattgtatt caatactgcc tgatcttttt
     3601 tcatatttac tattatatcc ttaagaaagt cctttgttaa ctatttcaat tcggctagat
     3661 tgtctccatg tgagacatga acatccaatg gaacttctag acttacaatg ttttttagta
     3721 tatctacagc ctttttctca aattcttcca ccctgccctt aactacttca aacactagtt
     3781 catcatggac ttgaagcaag atataggcat catcattgaa ttctttggat attagttcgt
     3841 ccaacttaac catcccaagc ttcataatat ctgcttcgct tccttggatt ggcatattta
     3901 cagcttctct tttagctgcc ttgttcattc ggatattctt ggatctaatt cctcctatca
     3961 ttcttgtagt cccaaacatt gtttgtacat aacctttttt gtaggcttct ctttccaaag
     4021 tcctgatata ttcctccacc ccgagatagt gttcgaagta attttggata tattccaatg
     4081 ccaccttgct atctatctta agtaaactac taagtccaaa tggtgtttgt ccaaaaagaa
     4141 ttgcaaaatt aacagtttta ccaatacttc tttccttctt tgttacatct tctatctctt
     4201 tttccaaaat tcttgccgct gttgctgtat gtatgtctaa tccatcgtta aaatccttaa
     4261 ttaacaggtt atccctagac atatctgcca taattctaag ttcaatctga gcataatcca
     4321 tacctacaag cttaaatcct tctctcgcta caaaagcttt tcttaacttc tccgaccatt
     4381 caccctgtgc gggaagattt tgcatatttg gattgataga ggagaatcta cctgaggtag
     4441 ttcctgtttg tttaaaatct gtatgtattg actggtcatc agaatccttt gccatagcaa
     4501 gcattggaga ggtgtatgtc ccatatattt tgcttaattc tctaaattcc agaagctttt
     4561 caacacatgg atgtgctcca gacagggaaa ctaaaatgtc ttctcttgta gagaatccat
     4621 tcttagatgg aagtcctagt tcgttgtaaa ggacatccga aagttgtttt ggtgaattaa
     4681 tattgaattc atgtcctaca gtagcaaata tttcttcttt tgttttctct aatttattag
     4741 ccaattcttc atttaattct tctagatatt taatgtctac ctttatgcct ttctcttcca
     4801 ttttagacag aactaaggca acgggcatct caattttcct tagcacatta agcaaatatt
     4861 cagatctaac caacagatat ttctttatac tctccaaagt gtattcgtac aactctatct
     4921 catttgcctt gttaatctgt gcatttttta tttcttcaac agtatctagc acagtagaga
     4981 tctctagtgg agatatcttc tctctaaaaa cttttcccac gtaatcaaaa gccaaatctt
     5041 tcagactttg gtcccttttc tctgagttaa ttatgtggga aaacagtgat atatcaaagc
     5101 ttttggccaa atctacatca attgtagtct ccttgtgtga aacaaggttt tcaaagttgc
     5161 aaaaaactgt ttcacagtta gcccgtagcg tgtaacttcc attttcatat gccctgtctg
     5221 tttttaccct catcttatcc acaaatctag taagtatgag ctccttgtct gtagtggttt
     5281 cttctttgct taaaaaacag caaacaatgc ttgatgcgtt cttagaaagg cttttaatct
     5341 cttcttcgct agcccactct acattacctg cactttcatt aaaaatattt agctgtggag
     5401 aatacccact tgagtctttt ccgaagatat catctaatct tgctaccaaa gatttaaatt
     5461 taaacctcct gaataaatcc agaacatttt tcctgtcaaa atcctgcatt agacaggatt
     5521 ctaaaaatat ctccaagtcc atatcttggt ctattttagc taattttctg cttagttctg
     5581 cctgctcaat tccctctcca agcaatacct ggagtcttgg tttaagtttt ggtaagtttt
     5641 tgtatattgt ttcgaagtct ccatattctt ttagaagctc tacaactgtt ttatctccta
     5701 ctccttttac tccagggata ttgtctgaag catctcctac aatggctttg taatccacta
     5761 cttgctctgg atatactcca agtgttttaa aaacattctc tctatcatac tctatgagat
     5821 tactaaaatt ccctgacggt aagcatacct ttacatctcc ccttacaagc tgaaacaggt
     5881 ctctatctcc actgagaatg taaagttcta gattctggtc tttccattta ccactatcta
     5941 ccatcttagc aatggtccca aggatgtcgt ctgcttcgaa gccctccttt tttatgattg
     6001 ggatattgaa agctttgaga acttcttcta caagtggaaa ctggtcaatg agagattgat
     6061 cgataggctt tcttgttgct ttgtaatcta tatattcctc atgtctgaat gttggtttac
     6121 tggtatcaaa ggcacagaga acatactttg ggtcgaaaga cttaagggca gaaagaagca
     6181 taactgtaaa accgtaaact gcgtttactt gaacaccatc gtctgtctga aggttcccag
     6241 gatatgcatg gaaagctctg tgaacaattg cattggcatc tatggcaagg aacctatcct
     6301 tagaggtttt gattaagttc atagtattag tctactacaa ctagaaaaga gcatcaaact
     6361 cctcccttac gactacttct ttttctacga gaatattaac taatttttca acatactcct
     6421 ttttctcttg gagaagcttc ttagaatatt catacgcctc atctacaaat cttttgactt
     6481 cttggtctat gagttctgca gttttctcac tgtactctct acctccacca tatgtatatc
     6541 caagatgctt tgtttcctcc atatctccgt atttaatcat tcctaacttc tcgctcatac
     6601 cgtactgcat taccatttct cttgctacac cagttatctg ttcaatatcg ttggatgcac
     6661 cagttgaaat ctctccgatg aaaagctcct ctgctaccct accagcgaca cctgttcgta
     6721 accatgcaag catttgcttc ttgtaaagaa atttcgtgtc cttctcagga aggtatactg
     6781 tgataccacc tgttagacct cttgatataa cggatacctg ttcaacagga tctgagcctt
     6841 ctgtaaactt tgcgacaatt gcatgtcctg cttcgtggta cgctgttttt ctaatatctt
     6901 cagcctcctt gtctcctgtt ttctttcttc caagcttgac cttcagatat gcttccataa
     6961 tatcttcttg actaattttc ttcctattct cccttgcagc cataattgcg gactcgttta
     7021 ggaggttctc taaatctgca ccagaatatc ccactgtttt ctttgctaag atatctaaat
     7081 ctaccccagc ttcaaagact ttgttctttg cgtgtacatc aagaattgct cttcttcctt
     7141 taaaatccgg catatttaca acaactgtcc tgtcgaatct tcctggtctg agtaatgcgg
     7201 gatctaaaac atctggtctg tttgttgcag caagtacaat tacattttgt ctttcctcga
     7261 gaccatccat ttctacaagt atctggttta atgtttgctc tcccgctcca gagtgcagga
     7321 ctgtgcctct tttttttgca actgcatcta tttcatctat aaatatgata catggagatg
     7381 cacttctagc ttttttgaaa agatctctaa cacgagaagc accagctcca acaagcattt
     7441 cttcaaactc tgaacctgaa gtgtgaaaga atggtactcc tgcctctcca gcgactgctt
     7501 tggcaagaag tgtttttcca gttccaggag cacctgcaag cagaacacct tttggtatcc
     7561 tagctccagc cgcaagatat tttttaggtt gtttaaggaa gtctactatc tcggttaatt
     7621 cttcttttac atcttcaatt cctgcgacat cttcaaaggt aattcctgtt ttctttccaa
     7681 acaagagtct ggctttgctt tctccaaagt ccataatttt accaccagat gcttgcatgt
     7741 tttttatcat gatgaagaca agaattaatc cagctccgag tagtacaaag gtaactatat
     7801 ccccaaaagt aataccaatt gctggctggt agaaatcatt ttctaaatct ttgatattca
     7861 gtcctgagtc ggagagtatt tggtagaaat ctgttcctcc tggaagcaaa gcatattcac
     7921 tcttcttgat atttccctcc cctttcattt ccaacgttac aattccatct ttaaggataa
     7981 tcttgtcata tttctcttcg gatatgtctg taacaatttt gcttacagat acttcttctc
     8041 ccctagtacc taggcctctg aagaagatta tggcaccggt aagggcaaga gatattacaa
     8101 ccatagcaat gatatttgga ataccaagtc ctcttcgtat tggcaatttc ctaatatcta
     8161 tctttacttc tttcttaata ttttttttgt tatccatatt ggtatattca aatttagagt
     8221 gcttttgatt atatcaaaag ggataactat tctaaagcta aacctacagg tatccaaatt
     8281 aaaattacag atacctgtag ggaagctcat tttcataatt ttttatctag attgctaaca
     8341 atctcccttt gaatctgatc gagaattttc tggctaggcc aaaggaaaga agtaacaatc
     8401 atattgattc cctctagcga aaaatccaga aaagcaggaa gtctcttccc aacattgaaa
     8461 gcggtcatcc tcgtgatttg ggggaaggta gttgcggaag catttgacaa aatctgcctc
     8521 ggggtttccc attgaggaga gctcaaacat ttccattcgt tccattcttc tggacaatag
     8581 atttggaaat gccaaagctt tttatccgta tgtggaaaac tgatatttag aatgccacta
     8641 tccccggact ggtatacttc aaacttccag ggttcctgag aatcctcaga ttttttggaa
     8701 agaacacgac tgtatggtga taaaattttt aacaaaaaaa cgctggtaaa atccaaaatg
     8761 gtttccattt ctcatccctt cttattatga aaagagcttt taaggatgca aaagcatcca
     8821 acgacagctc aattatacta caggatacat gaaaaatgaa tatggtgaga ggtggaatcg
     8881 aaccaccgac cttgggttta tgaatcccat gctctaacct tctgagctat cccaccttag
     8941 agttgaaata ttataccaaa gaaaatatca aaaacctatt tagttagcct ttccagagta
     9001 tgtcccagaa tctgtattaa ccttcaccac atcccccttc tttatgaaca ttggaacctg
     9061 tatcttgtac ccagtttcta atactgcctc ctttgtagca ctgtttgcgg tatttccctt
     9121 tacagcatct gatgtatcta caactgcaag aactactgta ggattttcct taatagttaa
     9181 aactttccct tcataaacca ttatcaaaac agtatctccc tctttaagaa aatttatata
     9241 ctcccctatg gtttccttgg gaattgtaat tgtttcgtat gtttcagaat ccattaaata
     9301 tacacttgtg ttatctgaat agagatactg catctccttt gtttcaggtt cgatatattc
     9361 aaccttagta ccagctttaa ttgtcttact tgttacatac ccactctcaa gagctctcat
     9421 ttttaagatg attaatcctc cctgtcttcc ttgtgttttt aattgtctat ctataagaaa
     9481 gtagaacttt ccatcttcta aaagatacat tccttttaca ggttggtttg ttaatggcat
     9541 aatgtgtata tactaaatta ttattgcaaa agcccattct aacacacctt tttaattaat
     9601 ccaagtatac atattcatat gacaaatcca aaagataaca cagtacaaag agagattctc
     9661 atttcctcag agcagataaa agagcgagta gcagaactag tgatagaaat taaagaagat
     9721 gtagatgttg ataatttagt tttagttgga gttttaaatg gagcatcctt tttcacaaca
     9781 gacttggcaa aggagtttaa taatccaaaa gtagaaatag atttcatgtc agtttccagt
     9841 tatggaaata aaacagaatc atctggagag ccaaccattc attgcgattt aaagaattca
     9901 ataatgggca aggacgtggt aatcgtagag gatattgtag atacaggata ctcgatggca
     9961 attctcttag agatactaag agcaagacgt ccaaaatccc taagagtttg tgcccttctt
    10021 tcaaagccgg acagaagaga ggttgatgta ccaatagatt atatcggatt tacaatcccg
    10081 aatcattgga tagagggata tggtctagat acaaacgaaa agtgcagatg ccttaagaat
    10141 ataacaagaa gagtataaaa ctggttgcgg gggctggatt cgaaccaacg accttcaggt
    10201 tatgagcctg acgagctgcc actgctctac cccgcgctat caaatctagt gattatacta
    10261 caatttgttg tgtactatca aattccagag gaaaggaaac ttttccaaac ttctaaacca
    10321 tactcactac tatccacctc attttcgctt aataacgaat caacatactc cctagcaaca
    10381 ggatacatat tctctggaat tacaaaaaca atttctttct taccagtaaa attcagtcta
    10441 tcccttgcct caacttcaag atattctacc gtttgcatcc tctcaagaag agatgctaat
    10501 tctaaattcc tcaatctcaa atcctctacc tctactctag ccttgtttaa aatctccacc
    10561 ttttggacag taatactcac tgaatggaat atactgtaaa caagaaaaac agatattccc
    10621 aaaagtacaa cctttgcaac agacgaatta aaaagagaca tgattacaat ctccttctcc
    10681 tgctttactc ttttatctgg ttgcctgtat attactctat ccatgtatag atattatcaa
    10741 tacagggtga tttaatcaaa gtaacccata tttatatatt attgctctca caactttatg
    10801 atgatataat tcttactata tagtagaaac tattaataaa ttaagctttc aaacacggtg
    10861 ggggcaataa acaatccaaa ttttgaggag aaaagagtca aatttatagc tgatctacag
    10921 agtaaaggaa gatctgaggc tactgtaata gcatactcca aagatattga acagttatta
    10981 aacttctttt ccaaagaggg gatttcttct atagaggata ctactattga ggatttagag
    11041 aaatataaac aaaacctaca ggacaataac tacaccccca aaagtatctc aagaaaaata
    11101 aatagtacta gaacatttta cagatatctc ttggataatt cattaataca agataatcca
    11161 tctgagaaat tagcacatcc taaatttgag acaaagcctc caagggtttt aacagagatg
    11221 gaatatagag ctttaagaga tgttagtcgt gtagatgtaa ggctctactc tatcgtagaa
    11281 atacttcttc aaacaggtat tagaatcgga gaattagccg cacttacact agatgatatt
    11341 agaaagtcaa acgatggaag gatagattac ctttacataa aagctgcagg aagtcaccca
    11401 cagagaaaag ttcctttaaa tagatcagca aagaaagcaa ttgatgagta tctggaatta
    11461 agaccagaaa cagaagatga tacactgttt gtaacaaaga atggtaggcc tcttttagtc
    11521 agaaatatta gaacagcaat agacaaatct tttgaaaaag ctggtattaa aaatgcaaag
    11581 gttaatgacc ttagaaatac attcatagca catcacttag caaatggcgt aagtcttgtt
    11641 gtagtatcta agttagttgg acacaagaga ctttctacaa cagagaagta tcttaatctc
    11701 gttaagatgc agaaggaaga tcagaagagt ttgaaagaat tatagagtta ttcttatctc
    11761 tcacttacaa attcaaatct atttactata gtaccatctt ctaattcttt agtccccata
    11821 aaatcttcca acgatctaat ccagaccctt gttcccttat cttctttata atacaaagat
    11881 gtgtaaacaa ccatgtcttt ttctgtttca gagtcttttc caactgtaag tacctgataa
    11941 taatctccct taaagtgctt atatactcca cctatcatta tctccctctc tccaatactt
    12001 tctttctcgc tcatatgaga tgatgttttt aaattagaat tggtgggagt agatggaatc
    12061 gaaccatcga ccacggcttt ataagaaccg cgctctaacc aactgagcta tactcccgta
    12121 acaaaagatt ctaccagaat atcatgtttt tttgaagata caaccctaaa aactcattgg
    12181 cggaaaaggt tgaggagtag atacaggctc ttccttccta tcaagctgag acctaatcct
    12241 aatatcctca ttaataactt ccaaatccct tccatacttt gctcttgagt acttccttat
    12301 catctctgct acctccttgt tttctggtaa atcaaaacca gaattgggga actcttcccc
    12361 atacaaagta tttaaagaga aaggcgcagg atacgagcct ttgcttagta acttaacata
    12421 acagtgccct attccctgat ttaccatatc tccagcttta atatatgggt caaattgagt
    12481 ctcgaggaaa tttgcatcct cagcactaca gcgatatatc attagagttc caacatttcc
    12541 aaatactgcc cccttaatat tctctggtaa ttgaccaata tactggtgag ctaatgtaag
    12601 atttaatttg tatttccttg cttcagaaag tatggatgca aattcatctg tagtaaaatt
    12661 ttggaactca tctgcataga aaaagaaatc tttcctagat ttttcatcta cattctccct
    12721 actcagggca gcagcaagta atttctggac aagcaaaaga cccaagaaag acatattctc
    12781 ctcccctatc ttacccttag agagattcac tattaatatc ttgccgttat ccatgacatc
    12841 tctaaagtca aaagacgatt tactctgccc tatgatattt ctaatggcaa ggtttgttgt
    12901 aaacttgtcg aatttagaaa taaagtatgt taaagactca gccttagtct tcttgtctgt
    12961 ctttgcgacc tggtctatcc aatatctctt cactagttca tcctgaatca atggaagcca
    13021 gtaatttgtt acccaatcct catcctgtag aattctaaca acttctatta gtgttgaacc
    13081 ctcctttgac atagctgtaa gcatagagtt tctaactgcc tgttggaacg ctggtcctgc
    13141 atatccttga ttatttgggt caaacaattt aatgagaagt gctataaaac tgttaacaat
    13201 ctggtgctta tgttgctcat tgtagaactc cataatattc aaaccaaaag gtctttcgaa
    13261 atctcccgca tcaaaatata taacatcctc tgctctctca ggaggaattc tttctagaat
    13321 catttctgcc gtttgaccgt gcggatcaat aaaacaaaca ccatctccat tatatatatc
    13381 ttgaattatc attctggtca tcagccaaga tttaccagta ccagtttgtc ctaagatata
    13441 tgcatgcctt cttctatcat ccctttcaaa acagattact ttcttctttc ccctaaatat
    13501 attgttccct atccatattg tatccttaga ttgaatatct gaactaattg aattgtctgc
    13561 tattaattct cttgctaaaa gccatttaat atttggcgta ttagtatctt tatttggaaa
    13621 atgatacagt gctgctaatt cttccacatt aagtatggaa ttctctgttt caggagttct
    13681 tctaaaaaca acatcataca ggaatttctt ttgttctttc tctgacactt tctttttctc
    13741 cagcctattt atccctggat tactgtactg atcaaaagca ccaacaatat tgtctaaatg
    13801 catcttagca atatcttcgc ttggtgcact tgtaacaatt ctaatctcag caattaatcc
    13861 tggttttgcg actttcttac ttatcccctc taatttttca tcagatatac caatcttttt
    13921 cttttccgga tctgaattgt tgtttttaat cttttgaaca aacttctccc cgtttttctg
    13981 ccactctttt cccgctggag atactgcaag ttggacaaga gctccttcat tttctgacat
    14041 tttacttaag gtactaagta cattcgccat cgggtctcca gtaaaatcac tagcaactct
    14101 taatggacag tacggatctt ctgcaagaat taatcttgta caagcagttt tggtatctgg
    14161 tgcaaatatg ttgtattctg tcacaaccct tacctcagca tcttggtaag aaccaagtat
    14221 ttgtttctca actaggtcag atatttttct tggacaatga acatagaagc gaatctctcc
    14281 tggaacacca atcacttcaa aggtaacaca gttgtttaca ctaaaatgtt ctgcaattcc
    14341 acttcctttt ccaccaatgc catataaatt agcaaacatt ttttctgcca cccctatctc
    14401 tgtttcattt ccacttggga cttgaacttc cattaggact ccctcacccg ctttttcatt
    14461 tctgtcttta gcaagaagtt tttttctaac atacaggaat actcccacac caataccaat
    14521 taagatgaga acaagcaaaa tccaacccca agggatgtta atctcttttg gtccatacat
    14581 atcaccatat gtatcttttg gatacaaagt ggaagtacct gtttctatct cggtattatc
    14641 ttctaaagtt ggaccaaatg tttgagcgta tatatccata ttggacagta agcaatctta
    14701 tggataaaga ataacatata tctgaaaagg actaaagatt cgcaattgtg ttcgtaagtt
    14761 tctctaggac aatttttgag gctccggtgc tttttggatc aatttggaac cctgcacttg
    14821 gttttggagt ttctatgcta acctctgctc caactgatgt ttctattggt tctgggatag
    14881 tgtccacctt aggagatact tccgtcatag ttgtggaaga gttctctaca gctccgactt
    14941 ctgtttcaat ttcagatgaa ataacaggag tttcaactcc tcctattccc attggttgtg
    15001 tttctattcg tgtattatcc ataacctata atgtctagaa ttataccaca aatttcctac
    15061 tttcatacaa cagatctcta tccatatccc tttccttctc tgcctttctc ttctcatggt
    15121 atttctttcc ccttgccaag cctatctcta ctttcacata ccctcttaca gtatatatac
    15181 ttaaaggaat taatgttaaa ttcccctgtt tcatcttgct ttccaactta tctaattgat
    15241 ttcttttaat tagtaatttc ctagttcttt tagaatcata gtgatcatca gaggcatatt
    15301 gatatttagt tatatctgca ttaactaacc acaactcatc ccctattatt tttacaaaag
    15361 catctgatat gtttaccctg ccactcttta ctgattttac ttccattcca agaagtacaa
    15421 taccaacctc aaaactttct agtatttcat aatcaaaacg agctttctta ttgataattt
    15481 tcatccctcc attatactac aacactatct tccatatggt agatccgtcc aaaacataca
    15541 tagcggactc cttgctgtcc actacaaagt ctttaacatc gctccacata ccatcctttg
    15601 ttcctctgta aataatttgg tttagaagaa gaatttgtcc tggatgtcgt atatctcctc
    15661 caccctcctg aggcttttcg aatttcaaaa atcttcttcc ctgcgcatcg aaaagataca
    15721 aagagtaatc tagacttcca gttgtgtatc ctttggttaa attaccatag tttccatcaa
    15781 atcctcctgt tgtaagaggc gaaagagctt gtttctgctc aaagtaactg tatgaatatc
    15841 taattagacc agaatccgat gtaagaaagt acaaactaag atccgaagat atgtcattta
    15901 cagtagcaaa attgtcgttc ctaatgtatc caaatgacaa tgaatagcta ttaccgtctg
    15961 agaaactaga cttgagcaat actttgtctg ctcttgagag aagatataca ttgtcagatt
    16021 ctgttaaaac aatcatctca gtaatctctt tctcttcaat atcgtctata ccaagcccag
    16081 agagtttaac aaatgttttt actttgctac cgtcgaatgg tgctttgaca acaccctctt
    16141 tagcatcata tacatatatg cccttatttc caacagagac atatttcggt tctttaatta
    16201 gagcatctgt atccaatatc ttttctactt ttttatcgta cagagacact ctgtatacac
    16261 ttttaagacc taaatctgta acaagtaaat atgaattacc agttttatct gtatatattt
    16321 caatatccga aggagcggaa ccttccccaa aagagagtcg tgaatctatg taagtgctaa
    16381 ttgacccatc cccgtctgag ataacttttc gcttaaaaag agtatcttca atatttaatc
    16441 ttctgtcttt caactcttgt agtttagttg tgtcttcgtc attgagttca gatggaattg
    16501 cctcaaaaag tttctctgtt tgaaacacag atagttcggc agcagatgta tctacaacca
    16561 tatctccctc cgctctgtct aaaagttttt caatcttagt aaatgattct tgtgctatat
    16621 tatgtctttc tcgggctttc tttgcattga ttgtgaagtt aattcctact gcaagaagaa
    16681 caattattaa aacaactacc acaacagttc taattctgga aactcttatg tttctttctt
    16741 tataaccatc aattttcatt cctttcatgc tttctcttct gtcaaagact ttattctcag
    16801 atattttgga agatactttt ttaaaccagt gctgttttcc aacaagtgat gtaactacac
    16861 tttttatttt agagactgct tctttgaaaa attgtaatat tttttcaatt aacggtttaa
    16921 gattgagagg aattttaaat ttcttttttt ccttttgggg catttttatt cttggttttg
    16981 gtatgtcttt aaggatatct gctttaactt caatattctt tacattatct ttttcctctt
    17041 ctgtattttg gaaatatatt attccttcag aatttaaaat cttctctcca aacttctcca
    17101 atgattcgag gacttcttgt ttatttttgt gtcctgtaaa tgttgccata ccctcggtta
    17161 aaagtccggc tgtggagatg agaagtatat cgtcttgtgc aagagctata cctgctgtac
    17221 tagatttttt ctccttgaga acatcatata tatttataaa tctgttatct ttgtaaacat
    17281 atatatcgtt ctctccaaaa ttccctagat acaatccctc tttcttttgg gcaactacaa
    17341 caaaattaat attaacaccc tcctgctcta atgtttggtc gtttctgatt aaatctttaa
    17401 ttttgtgaat gccctctttg atggaatctt ttagagactc atttgtagtt ttagcgttgg
    17461 aatacagata tccatctaca agaccatccc atacaaattt gcttactcgc tcagcagtaa
    17521 gatttttact tccagaaatt gaaagaagag catacaagtc tccctcttta ctaattttct
    17581 tttctgtgag gttcaagtag tgcttaaaga cggaaacgaa ggtgttttta tccttgtcgt
    17641 ttgatacaaa ctgttgagcg gtaattttca ttttagccaa ttaacaaaag tagtaatgct
    17701 attatactcc ctattacact tataaagaga tagaagcgta ctaaatatct gattaatttg
    17761 ttgtctgtgg tctcgaatgt ctcagagaga attctaattc ttaaaagtag gaggactgcg
    17821 aacaaaacat ttccaaaaat aataatgatt agtggtagct taagaaggga gattataccc
    17881 aaatttatcg tgtcagtggt agtcccagat atgttgttta atatttctaa tatgttttcc
    17941 actatatttc tcctttctgc ttaagttcaa gcaaatcaaa aaggatgtct tgtacatctc
    18001 ttggtcttgg aacatttgta aattcaattt cattttgagc tcctgctgtt tgtatatata
    18061 ctgaacctac atcaaagatg cttcctattg cgcccaactg tttgtgggta acatcctcta
    18121 tcctttccaa ttgagcttct gccatagaat gattcataat acttgtaaaa tctaaatcta
    18181 ctaccttttg gtctgtaatt atgtgcactg tgtagaacca tttaacaaag gtgcttacta
    18241 ctattcctag agatactagt aaggagataa taactaaaga tattagaagt gcaatacttg
    18301 agaaaaatgt tggcattaat gatgcaagaa taaagggaag gataaaaaca agaatagcaa
    18361 ggaagaagta tggaactgta acaatccaat gggaacgaat aattaaaaca acttgttcac
    18421 ctttttcttt tccgtggaag tttaattcct taggaaacga cataaaggag taattgttgt
    18481 ttagagagat tgtatccatg aaagatttct ttatatttct aaactttact ggaattttgt
    18541 tcttatcttc tttcatagcc atatgtagat aaagataaca tagtattaac tatataacaa
    18601 ggttattata gctttttctg tatatcaatg tgaacaaatc cagattgtgg aatactcaaa
    18661 tatcctggtg cacgaaggtt gtaagcaagt ttaaagtaac ttccagagat agtaatctct
    18721 ctcccatctt cgtttacaaa tttaatagag tttgtatttc cactgctcct agatacctgt
    18781 gctagaatta cttttgtgaa ttttgaagaa gatttgtttc tcatctctgt aaaggagtat
    18841 ggattgccat cgctatggca tctatcgtgt attggatata ttctagaatc acttgtacca
    18901 ctacctccga tgtctgttaa gcgcgatgct gcattaacaa tatctgccat ttcctgtcca
    18961 gagagccatg gatacctata acaggtggaa ctactcgttg atgtacttaa gttatagtct
    19021 ctcctgtacc aagatctata tgtccaagaa acccctgatt tattatccca tgtgtttgtg
    19081 aaatcttttg ttccactacc atcgaccaaa tcccaaccaa tagtgtttac ccatccacca
    19141 tgtactgcgg cgtactgagc agaaaagact ctttttgagc tatctgttaa aatcatccct
    19201 ttcgtttctt tcactgctgt agcccaagct cctgtctttt tatttgaacc aataacctga
    19261 caattttcat ttgtacaaat tgctttgcta ccatttgagg tataatttat ggcatatgtt
    19321 ctcgccacaa tagcctgtgc ctttaacacg ttcattcttt ttagattcaa agacgagcta
    19381 ccggtttgtg tccaatcatc tggaatctct ctcaacctaa gcatgtagtc atcctcaaaa
    19441 ttcaattctc catatgttgt ggtgtctatg tctgtcataa tattcttaac cgttcctcct
    19501 atgttgacta ctccggttgt aattctcttg gttggataat atgcactaat aatctgagta
    19561 tatgtttgtc cagcatctgc ccttgccctg gccccccatt gactcattcc atttctgtga
    19621 gtatatgcac caatcgaaaa cactccaaaa gaaccaacgg gtgctttgct agtgaatgta
    19681 gataaacttc caagatcgct tcccccagct gtaacctgac ttggatctac agatgtacca
    19741 ccctgccttg tatagagtaa tgtcttttgc aaatttgata attgagaagt taatttatta
    19801 atttggctac ttatacttgt cctagaagca atctgtgcat ttaattcctt ctgtaacttt
    19861 gctttctctg cagctaataa cgcatatgat ttatctaaat ctcctttttg ctcctctaat
    19921 tctgtcttct gcttttccaa tgcctcccta ctctcagcaa gagatataaa ttccccatta
    19981 attttctcaa cctcatctct caatacagta attgctctct tcttaacaaa caacccctct
    20041 acaaaaccat cccaactatc tcttgagaga aaaaattgag acattctgta tctagattgt
    20101 atatacaaat ctgaagagaa agtatctatt gattcttttt tcttctcaag taaaacttgc
    20161 ttttcttcta tctcagcaat cttcttgttt aaatctgcct ctgtactatc tatgctcttc
    20221 tttaacttgg taatctcatc atttatgagg ctaatcttct ggcttagtga atagttacta
    20281 ccacttatct cagcaatctt tttctctata tctgaaagta ttcccttctt cttagctaat
    20341 tcctcattag tttggttaat ctggttttca agatcctcta tctcacctga atatgctgga
    20401 aaattgaaaa gagtaacaca aaaaattccg agagaaacta caagaaaaag ctgaattata
    20461 ttttggggct ttttaggcat attcccatta taccagaata ttttttacaa cttccattat
    20521 gtatgggagg aaaacaatta gacctgtaat gattgatacc aaggcactaa ttaatactgc
    20581 cccagcactt acatcttttg catattttat attcacctta aaatcttgcg aaacagtatc
    20641 gcagagtgcc tctattgcac tgttgagtac ctctgcagta aagaccatgg agataactag
    20701 actaatagct atccaatctg tgtgagatat tttaaaaaga aatcctgcaa ttacaactaa
    20761 gacagcaata actatgtcta ttctaaaatt tctttcatta cggaagatta acttaacacc
    20821 atttctagca taaccctgac ttttaagaaa atctggcgat ttaaaatggt cgtttttcat
    20881 ttaagatata aatttcacga ttacttcaat aattgcaaca gctaaaagaa cgacaattaa
    20941 accaatgatt gcccatgtta gagtactttg tgcactctta accctcgatg gatcccctgc
    21001 tgcagacatc cacatatatc caccaataat tacgaagact acacatatta atcccgcaag
    21061 agggaatata tattggagta cattatccaa gacatcaaac aattccccta tcttcttcgg
    21121 ttcctcttcc tgtgcaaata caggatttgc aagggaaaga gaaaacaaaa agactagttt
    21181 tttaaatatt ttcattattt tattcctaaa atttctctga tcacaaattc tataattgat
    21241 ggggatataa atacaagtac caatccaaca atagagaata tcagagacct agaagcttct
    21301 tttactttct tctcatcacc aatagaaagt ataaacttga atccagaaaa tatgattgat
    21361 ataacagcaa gaactacaga aaatgatata acaaaaccta tgagagaata caaaaaatca
    21421 gaaagattct caaagcccgt attattaagg aagctcaagt ctatgaacat gcaatttctc
    21481 cttccgtaaa ttagctatta cttaaggaca ttcttcaata caaattcgac caatataact
    21541 gcaatgaaac agattactaa tccaactatt gcatatgtca aagatttacc agctttctct
    21601 accttagttt cgtctcctgc agctgtaata tacatatatc cactagcgat taagatacca
    21661 acacatacca atgcggctaa tccaacaaca agatttatca caccaccaat ccatgattcc
    21721 aacggttggg taacaccacc tgtacctttc aatctatcaa cccaacttaa agcggtctgc
    21781 gcataagttc ctgatgctaa atacagtcca gaaaatacgg aagaagcaat tgctaatact
    21841 tttggtgctt tcatttttta actactaaaa tattaatata ttaagtttat tatatagaaa
    21901 gttaagttaa cttgcaatac tttctatttg actacaacct taagaacaaa ctctaccaat
    21961 attcttgcta taaagacaat tattaatcca acaatagttc cagacaatgt cttaccagct
    22021 ttatctacct tgtcaggatc tccagaagag gttatgaaca tataacctga aactataatt
    22081 aagacaacag ccactaaggc agcaaatcct gtaaagaagt ttatagcatc tgcaaccttt
    22141 gttccgatta aatcttcgat atctgaatcc gaaccaaaag ttggtaagag tccacttact
    22201 atacctgata tatctatttt actcatatat acctaaccct agggtattag aaattaatat
    22261 taatattgct acggagagta gcactactac aaatccaata attgcatttg ttattatatc
    22321 ctttccctct ttaagtttat ctggatttcc ctgactagat gtaagaagat agccagcata
    22381 tacaacaaga ataacaacac tgataactcc caaaggtact gcaatgctaa caatcgtctg
    22441 aacaagatca ctttcatctg taactgtccc atcttctatt ttgttcaact gatcaccaag
    22501 agaactaccc aaactctggg catgtacctt ttcaaccaaa aacatattta atgtgtccaa
    22561 aattctcata gtgccaatta tactacatta tattaaattt ctgcgaattc acaaacggaa
    22621 ttgtaatcac agaaattaca tccaaaccct ggcgttgctt taaacttcct ttctcggata
    22681 ttccctattg cttccaatag cttctctttt gccaaatcct tccttttttg tgagatatct
    22741 acctctatct cttccattga ctccacaaat atatattttg cacttacaac ctttaatcct
    22801 aacttctcct gtgcaaatac agcatataat ggaagttgta aatccttttt aatatttgcc
    22861 tcgtttttta cctttcctgt tttgtaatct acaatacaga cctcgccttc ctcacctacc
    22921 atatcaatcc tatctatctt ccccacaaat acactctctc caaagtgtac cgagaaagat
    22981 tcttccagtt tcaatggctt ttgttgttca gaatacattt tgttgtaata ctccttcaag
    23041 atttttcttc cctcttcttt ccttaattgt tcttgtttag tactttcgta gcctgtactt
    23101 acccagttct tttcgtagta tccaagtaaa tcttctagtg ttggaggtgc ataaactcct
    23161 tccaatcctt ctttggagtg ttttaatgca gtgtaaagtt ctttaagagt attgtgaatt
    23221 gttgttccaa aagacaaaga tgaatttggt ttttgaggaa ctttaagtac ataggaatac
    23281 tcatatttcc ttggacaatc ttcgtatgta tgtaattgtg aatagctaaa gtttttaatt
    23341 acatccaatt taacttcttt aggaacaatt gattcgtatg tatccaaatc catgcttgca
    23401 attttcatcc ctccatcttt aattaatcca acaaattcat cttctacatt tctgtctagt
    23461 atttcgttta ggaatatact tggctttttg ctagatttac catcaccgta atatttagca
    23521 gcagaaagat acaatttctc ttgtgctcta gtagctccaa catagaaaag tcttctttcc
    23581 tcttgaagat gtgaattact ctcagaaaaa ttctccccca atgtttcctt aattaattcc
    23641 tctggaattg gtattgcatc tgacctgttt tgtgacggaa atctctggga aacaagatta
    23701 accaaaaata ctactgggaa ttccaaacct tttgagccgt gcactgtaag tatatttaca
    23761 ccattaaagt cttctaactc agactgatcc accactgggg attctccaac ctccatacag
    23821 tagtttagat agtctacata ttcatatatg tttgaatctg gattatcttt ctcatatttt
    23881 tttaccaatt caaaaaactt ttgtatattt gacactgcaa agagattctc tccactctct
    23941 tctcccaaga aagaatctag atatccactt acctttacat aatcgtacag gatttctagt
    24001 ataggtctgc catctttaat cctttttaga gagctatcta ctatctcaag caaattactt
    24061 atcccagcta ttgccttggc atctagcatc ttgaggacta acggattgct tacatcatca
    24121 atttctccct ccacctcacc aatctttaca tcccaaagtt tttctaattc ctcaaaaaga
    24181 gttaatttct cctctctggc tagtctattt atctcaatat attctcttac agttaaattc
    24241 caaatattca ttgaaagcaa tctgtacata gcaatttcat ctgagtaatc gactaagact
    24301 ctaaggaaag caacaatatt ttgaatctct ggtctaaaat acaatcctct tgctccacct
    24361 agtttgtatg gaactccttt gtttcgaagt tcctggataa agggctctgc atgggaattt
    24421 gctcttacca gtattgcaat gtcactaaat ttgaatttgc ctttctttga aagccctaga
    24481 aatcctgctt gacctttctc gtcaaaaatt tgtgctccac taccctcttg tgcatgctct
    24541 ccgtatcctg taagtttaag aatctctcct acaacccttt ctgtttcagt atattcatct
    24601 tttgcagcga ttaaagaaac aacctcttct tgtggttcaa aggctcctct tgccacaagt
    24661 tttttgtcta tcttttctgt ttcttccaac ctgtttggat tgttgtgttt aataagagca
    24721 tatgcagcat caaggatttc ttgcctggat ctgtagttat ctgttaaaac aatctccata
    24781 gcctctggat atttttcctt gaattgaaga atgttggata ttgcagcacc tctaaatttg
    24841 taaatggctt ggtcatcatc gcccactaca gttagtttgt gtcttttacc atctttgagg
    24901 atattgatga ggacattttg ggtatagttg gtatcttgga attcatccac aagaatatat
    24961 ttaaactgtt ttctgtattt ttctaaaaca tctgctcttt ctctaaagag tttaagggtt
    25021 aggattatta aatccccaaa gtcaattttg gagttttcaa tttttaactc agaatatttc
    25081 ctgtatgttt ctgaaagctc tttgtatttc tcaaagctag ctttctcttc tggtgttttc
    25141 ttaggtagtg cttttgcata tttaatatat tcctcatctg agacatcctc atcttgtaat
    25201 ctggagaagt gtttaattag atcgttgaga aattttgttg gat
//
LOCUS       DAOQWP010000012        22319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_2684_length_22319_cov_7.193272, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000012 DAOQWP010000000
VERSION     DAOQWP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2684_length_22319_cov_7.193272"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..741
                     /gene="pth"
                     /locus_tag="PKI16_02185"
     CDS             178..741
                     /gene="pth"
                     /locus_tag="PKI16_02185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNR53259.1"
                     /translation="MKLIVGLGNPGKEYSNSRHNSGFIFLEALKDKFLYQKDIYATEW
                     RIEDTFQSEISFVKRGSTIVAVLVKPMTFMNKSGEAVSKIAKKLEIDDIYEDMVLVHD
                     DLDIALGEFKIQIGKSPNGHNGVKSVEDRLGTNNFKRVRIGVENRQEKSIKGEDYVLM
                     KFTKEEKVMLDETIQKAIQGILVDIIW"
     gene            complement(731..1933)
                     /locus_tag="PKI16_02190"
     CDS             complement(731..1933)
                     /locus_tag="PKI16_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNR53260.1"
                     /translation="MFLAHGPVSYLANEIIQKKKISNLKFHEQILVSILAILFGILPD
                     IDLLILPMFSIPQFSHHNYFTHAPLFYIGIWLILKLKVAIFDKITSKRTSAVLHHDLM
                     DVILNTFLIATMVHFIADTLTGGIMWIYPISTFKFSILSSLMTPSLFQQYLLLPDFAV
                     ELVLCSLFFVHIYKKFFQKNIFFDVLKYFFLSVSTIFLFVTVFFSTQTYNQSKMYDSK
                     GKENYDVDMDGLNDKKDLDIDNDGIDNIVDIDLKKLTKEVKDISESGKLASFDTNSIQ
                     YMFGGFNSYRLLSQAYFNISSPIEGALRNTVEEREYWTKLSYPELLYGYFSSKTLLTE
                     LDVTQESVLNIGTLIFLLDEDDSVVNIGLVVDEDSVGVVLPQDTKLTTHTLQELFESE
                     YTRVMVTK"
     gene            1999..4380
                     /locus_tag="PKI16_02195"
     CDS             1999..4380
                     /locus_tag="PKI16_02195"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNR53261.1"
                     /translation="MVNITKELSKNLGEIKEGVLKSKKVSGIEPYLLEFAAELLGNIL
                     NKKIFLRDSGENLQKKLLTIKKGDMFSISNLLDLGYTRVERVWCEGEISILGDVVVVW
                     PFSTNNVLRISLLGEKIEEMAVVDSQTRKKIKNVNERVFVSEGSKIYLGNEDGDSEEI
                     LDIVPRVSEEEYVDLELRSIPGIENFSNPKVLKEIVKNFRSRKFKIWYVTDKGERVEK
                     LVQKDVLEMIDDIFTEERNISARGFVSQRNEVVVLTDLEVLGEIDLSNFEKVNKSLDP
                     NSIEILKKVTPGEYLVHEDHGIGKFVGVIEREKGFYIEISYAVEDKLYVPLSASEKLT
                     KYIGAGKGKPVLTGLNSGSWKRISQKASEHAEEIAKELLMLYAMRETAQSVFTLEDED
                     RESFKTFVEQFEYTDTDDQILATDQIEQDFKRGRPMDRLLVGDVGFGKTEIAMRAMFP
                     VVNSGFQVAFLAPTTILVQQHLTVLRERFKNYPFNIQSLSRFSTEADKKRVLHGLKNG
                     VVDIVVGTHSLLSDDIQFKNLGLLVVDEEQKFGVKQKEKIKGMRINSNVLSLTATPIP
                     RTLNMALMGVRDISVLAVPPSGRKDIVNSFEHFNWDSIVKAIQKEMDRGGQVYFLHNR
                     IGNILSIQKKLQELFPKAVVDVVHGQMGSKYLSERMSDFVSKKTDILLCTTIIENGLD
                     IPNANTLIVDDASKLGLSQMYQIRGRVGRSLEQAYAYFFFDTLSENAILRLDALRDSQ
                     SLGSGFLLSNRDLEIRGAGDILGKNQSGTINSVGYGLYTQMLSEAIEKIKARG"
     gene            4463..6028
                     /gene="dnaX"
                     /locus_tag="PKI16_02200"
     CDS             4463..6028
                     /gene="dnaX"
                     /locus_tag="PKI16_02200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNR53262.1"
                     /translation="MSSVLYRKYRAQDFDQLIGQGHITKLLKNAVKTNQISQAYLFVG
                     SRGTGKTSAARILAKAVNCLHPKEDGNPCNECEVCVGITTGRLMDLIEIDAASNRGID
                     QIRELKEKIEYSPTDSKYKVYVIDEVHMLTTEAFNALLKTLEEPPAHVIFILATTDVH
                     KLPPTILSRCQRYDFRLGTNEEIEKLLKDVAVKEDIKIAKEAIDILVQNAKGSYRDAL
                     SLLDVVYSGRGEGIKNISERDVRDILGLPDFDLVISLLKNLIEGSGKRSLEVIEDLEK
                     KGVNLAQFTTYALEILRNILVEKIKGKVSKEYAFAKDTDLKTLHKLINLFLDAENKIK
                     YSSNQGLVLEMIIPEMIKVDSEEIKIVNIDVKNKIQTTEGQKETTEESEEEEEEDNVT
                     DESISVEDVRARWEKFVNEVKPFNGHLYAFLGGARVISFDENTLLLEVPFAFHKDRIE
                     IPRSRDIISTTFHKVYGVNCKIVCNVNKDVKPKRKSEAEFVLRNLPPAPPKKEAARSR
                     NMKKEVEAIFEGM"
     gene            6091..6381
                     /locus_tag="PKI16_02205"
     CDS             6091..6381
                     /locus_tag="PKI16_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNR53263.1"
                     /translation="MINPVELLRMQNEAKKMQKKLRERRITGESKDGLVKLYMNAAQQ
                     LEDLRIDDQLLSPDMSDELRKYFKDAFKDYQKKLQKEMVADMDLSQLKNMLG"
     gene            6383..7006
                     /gene="recR"
                     /locus_tag="PKI16_02210"
     CDS             6383..7006
                     /gene="recR"
                     /locus_tag="PKI16_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNR53264.1"
                     /translation="MSVLPKSVEQVIDEFSKLPGIGPKSASRMVYHFLRSPNKDATKL
                     AKALTQMDLEVKNCSKCFNVSDKDICDICSSKIRKTNKLCVVEEPLDVVAFENSAVYD
                     GFYFVLGGVISPADGIGADEIRFTELKDRVEELLKENDTVEVIVATNPNLEGEATSNY
                     ILDLFKNLLKEGKIKISRLAMGLPTGADLEYADRLTLKRALEGRRDI"
     gene            7006..8439
                     /gene="cysS"
                     /locus_tag="PKI16_02215"
     CDS             7006..8439
                     /gene="cysS"
                     /locus_tag="PKI16_02215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNR53265.1"
                     /translation="MKLYSTLDKKVVDIIPIKEGHISMYNCGPTVYYRMHLGNIRAYV
                     SWDILHRALRYLGYDVKRVMNFTDVGHMTHDEDFGEDKMDIEAKKEGLQAIDIANKYI
                     NTVLEDFKALNILTPNGEIIEENLDFKDVEKHGWMRATTHIAEIIEIIKKMELNGYTY
                     ETENALYFDVTKIPDYTIFTGQKLEDKKVGDREEVVVDTDKKNPADFVLWMKKVGKYE
                     NHEMFWDSPWGYGFPGWHIECSAMGTKALGENFDIHTGGVDHIPVHHTNERAQNIGAF
                     GHGVVKYWVHNEWVVNKDNAKLSKSDKNADTLPEIVEKGFDPLDIRYLFVSVNYHTKL
                     AFSTEALEGARNARLNISKRVKELGTENGNLLDGYIQRFRDALENNLNMSEVLSILNE
                     LLKSSNKKEDILATVLEFDKVLGLGLDKIEDTKIDLGKEIEEYVKLRDEARANKDYTK
                     ADEYRKRIEDAGYVVLDTPQGTKYQKK"
     gene            8440..9336
                     /gene="rnhC"
                     /locus_tag="PKI16_02220"
     CDS             8440..9336
                     /gene="rnhC"
                     /locus_tag="PKI16_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HNR53266.1"
                     /translation="MIQNTVTIQLDEIQRENAEDVLEAYGWKEEYVNNPYAKFRMKSP
                     QSSNVILYTSGKLVFQGSEDFTSIITAIKNNREDESDIIPHLGVDEVGKGDYFGPLVV
                     VACFLDEEFLEKISSIGIGDSKKFSDSKIMEMYNLVKDHPYFYTSIVYPKEYAQLYKE
                     SKNASILLARQHSKVIEMGLGDLKSKNIECSRVVIDQFSSRKSRILNELGSYGKEIEL
                     IQFHKGESDMAVACASVMARGIFLEEMEKMRKKYNFNFPKGASDVVDKGQDFVKKFGA
                     EELGNVAKISFKTTAKVLQTKL"
     gene            complement(9326..10231)
                     /locus_tag="PKI16_02225"
     CDS             complement(9326..10231)
                     /locus_tag="PKI16_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53267.1"
                     /translation="MKIKKIFTIGFLSILFFLPLTHVNANSLAPAITQSMLSSGDTFD
                     SKILFTNTTNKEVYILPTVVGYNPKTSSIITENTYLFVKTDKDTFKVEPNATISLSYQ
                     IVVPSNLPLGTYFNLIILKQTNDAGFINPQNSVGAVDNLSHLVVLHVVEQGDVKGITS
                     EFAQISMEIVDRGIPFIRDTKVKYIYQNITNYVLVPDGEIQVYNNKGKYAPKYYKINR
                     DKQKLYPNDIIEETIVIDEWHISDLIYPRTVLGLFYNGIDENSVTKEIQQSSSYFFLI
                     TGVIILTLGFALFKSLKEDRKKSKV"
     gene            complement(10237..11247)
                     /locus_tag="PKI16_02230"
     CDS             complement(10237..11247)
                     /locus_tag="PKI16_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53268.1"
                     /translation="MKKLNKDAAPYSKNIIYFVVSVFFSLFSFCLFNPLNIYAAPLSI
                     GTAPTSETLNLQPGDKYHGEIVIWNLSDTTTTYDVLVTGFRQIENQPGTAIFLSEAEE
                     AKNLYSAASWVKVDRKVIELVPNRNEKIYYDIVVPKDATKGEYNVIIAFKSQTENQQI
                     IGTGTVSTLASGTPILIKIGKDFVENAELLSFTTDKNFYEFPNVVFTTRIKNLGDTHI
                     SPIGEIVLTNIFDKEIARIPFNENTQSLMRENTGNYESTWDFGKFLTNDKQIVLGPID
                     AKLVATYRTFQPGFAPLTAETSFWVLPWRYIVVALLIITLLVIIVKTAKKKKAEYKPL
                     PK"
     gene            complement(11255..12391)
                     /locus_tag="PKI16_02235"
     CDS             complement(11255..12391)
                     /locus_tag="PKI16_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53269.1"
                     /translation="MKKFRALKSVLLFALITSLVTFFVSTLSFQSKAASLSDLYVYLS
                     RLEANVTGSAETLEIVLAINTTTTIPTGGTVTLYFPDADDAMWCRTAGTLTVAGVAAS
                     AADLAATDWDIDEVLPTSGTLAAACTQGSGGSSVDTITISNVGSLTAGTTYGVKISNG
                     TTAGVIGTDDTTGAHEITVEAKSGSTIDSSTIKVSLIANDTVVVSATVSATPSVNCTI
                     SANTVNLGTLYPGGSYTTASTHTIGTDTSGTTAGYYWAAYGTGNGTDAGLYKSTPTTY
                     LIASTGSTTVNLTTAGSEGFGIALSDPDGASEATVATNFSNATPGVYGALDPLPAGAR
                     MILYQNAAQPSNETSTVTYGARAGATAVAGAYQESVIYVCGGYY"
     gene            complement(12478..13686)
                     /locus_tag="PKI16_02240"
     CDS             complement(12478..13686)
                     /locus_tag="PKI16_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53270.1"
                     /translation="MKSANIFKTVSLLLIIVLSSTVYVFAEDLDSTNYKIVGAKTATG
                     GGLGDSTNYSVISSLGKVSADPRNYSTNYRLYQDPSAAFLAAQPTLQCFETTTDGSTA
                     CTSGPQELLDGGMVAICGTGGCYDKARFEISASSNPSDTLYGVQISTDNFVGDIQYID
                     GSTFRPESYANHNINDYRTESIWETEVFNIQGLSANTQYYIRLTALHGDFTESDYSIA
                     ANTTTSAGSISFDIDIAIASGIDTESAPPYSVGFSSGTALIAGAAATTATSLAWLDID
                     SSATGGVSVIVFGKYGGLHSATTTQTITSSTEDLDVTGAEGFGLQSYYIDYDDSSPYY
                     GELTATTDYSGSINSVGGVATTANKIYSGDGPIKAGRTGAYLKARASTGITPASDYSE
                     EIYFILVPLY"
     gene            13729..14703
                     /locus_tag="PKI16_02245"
     CDS             13729..14703
                     /locus_tag="PKI16_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNR53271.1"
                     /translation="MKEAVVSVKNLSKTFRIFKSKGISSLFNRKYENIKAVDDISFEV
                     YKEEFVGFIGQNGAGKTTTLKCLTGLLTADSGEVEVLGFNPKKRKHEFLSRISLVMGN
                     KSQLWWELPAIETFKLNKEIYEVSDEKFEEIVLEMVTLLGIEEVVNIPVRKLSLGERM
                     KCEIVVSLIHTPELLFLDEPTLGLDVVSQQRLRDFLREYNKRYKASIVLTSHNMEDIK
                     DLCKRVIIIDKGKKIYDGKVDDLARNFVKSKEIVFSLSSVATHKEIEKVGKIIEFSGL
                     SGIISVPRESVASASSKLLNTFQVEDLNIQEPSLEYIVKELFEGRYKV"
     gene            complement(14839..15468)
                     /locus_tag="PKI16_02250"
     CDS             complement(14839..15468)
                     /locus_tag="PKI16_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNR53272.1"
                     /translation="MIIFIDESGDAGFKTQRGSSKFFVIALVIFDDELEAEKTAIKIK
                     DFKVALGKPLNYEIKFNNLKKSDKLDFLEIIKTCKFRVRAIVVRKEKVYSTNLRTDTK
                     IYYNFFLRQVLEHNNATIKDAKLRLDGFGERKFKKAMGSYLRQSLNGRIRNKVMNNLK
                     FVDSKNSVLIQLADMVAGSIHRSYQEEKYDSKIYKEVIKKRIEDEWIFG"
     gene            15557..16093
                     /locus_tag="PKI16_02255"
     CDS             15557..16093
                     /locus_tag="PKI16_02255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNR53273.1"
                     /translation="MLTITYFVHGTTIDNEQHISSGWKDVDISKKGIQQSKELPLLNT
                     TKFDTVFCSDLLRAIHSTELSFKDKYPIVIDKRLRECNYGDYNGKPSDIVEPLQEQMI
                     YTRFPNGESYEDVKERIGLFLNDIQHKYNGKHIAIVAHKAPQLALDVLLKNMTWEEAF
                     KNDWRKTGKWEAGWVYTL"
     gene            16150..16236
                     /locus_tag="PKI16_02260"
     tRNA            16150..16236
                     /locus_tag="PKI16_02260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16184..16186,aa:Ser,seq:tga)
     gene            16252..17865
                     /locus_tag="PKI16_02265"
     CDS             16252..17865
                     /locus_tag="PKI16_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53274.1"
                     /translation="MGQDTKTVENIYKPKDRDGGFPSGMSYFKKRIYEIIPGLFVWVL
                     LLLPLIFAIFKWEEALVIYIAYLVTYWAYRTVKFVVGGYIGVRRMKRDISTDWMKKIK
                     EVNGARFNELRYVYLCPVYSESLDILIPSFESWANSDVGAKKIDVVMAIEERKKDLQI
                     ENFKKLKERFGKKFGSMQYYVHPADIPGEIAGVKGANINWAARHFVKDLEKEGKNIGN
                     YMLATCDSDQRVHPKYLSAITYKYLTVPNPDLGYYATAVHTFNNNIWNVPSIIRTQSN
                     MLTLVILHLWVLTKSLKVPFSRESINVRETFSSYVVNLKTLKEMEYWDPEIPNDDTAF
                     FWNTMVRSKGLAKSHEVYIPTYNDAVENETYFKSHVSFYKQQYRWGWGIFTLPISMAA
                     LSQDKENFPAHRKFALLKTMFEYLWFLTVVFVLTFGLSIMGWVNPGFQYTGFAYNLPR
                     ILSYIFTAIMLSNIAVVIYRRQITPVPKGWKWWRHLLDFLETYLIAFNMLTFSFIPYI
                     QAMTEMMFGKGRFKRNFYVTEKVKIKEKR"
     gene            17922..19061
                     /locus_tag="PKI16_02270"
     CDS             17922..19061
                     /locus_tag="PKI16_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53275.1"
                     /translation="MFVLLFLFLFSSPIFADDFNILGDSGPNEFFQATALTTESFPVQ
                     GKISFDENELLGQNTTNILNTQSIFTLANMGYTRRIPNIADKVYGPPGTKIDLLYFYQ
                     NNTGHSVNISEIAVPLSRSIVSNGDISRTLNIVGVERNVEFLFSGNNLSRGGKYVSYD
                     GLGEIANGVGGGILLDSWYIQDPLTVELLKAERDLDIVNLKVKVKNITEDEYFTNLNF
                     KHKEYQEVFALLPSEERYLEYSLKNEEKILEPFTLTNPNGKERCAVLGGNYYQWYFTD
                     SISVFAKRENDKWVGGGYVQPAVESICVKRIGYELKSEEIQLESREILGVADDLKELP
                     QTNSSSFLCLLLFVVLDVFLWYAFLRRKKKYEDKNSNTRLCSKSR"
     gene            19015..20439
                     /locus_tag="PKI16_02275"
     CDS             19015..20439
                     /locus_tag="PKI16_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNR53276.1"
                     /translation="MKIKIVIPDYVQKVGRMLGKEGFACYIVGGAIRDLVMGKIPHDY
                     DLATDALPDEMLNIFPKSVSIGAKFGTIMALVQDGNGETHEVEVTTFRSESNYVDGRW
                     PASVKFVEDIDLDLGRRDFTFNAMAINLADVELDGMEIEKEVEIYDPFNGLGDIKGTL
                     IRAVGTPLERFKEDGLRGFKACRLAAQLNFEIDEETFKAIKESLPIASQVSMERIRDE
                     FMKMLLNSSKPSIGIELMRQTGLLNIFMPELLEGVGVEQKYYHSDDVYWHSLRTCDSA
                     EDSVKLAALLHDIGKPRTNMGNGHFYGHDSVGADMAEVIMKRMKFPQSEIKRVLILIK
                     NHMFYYPYVTEEMSEEDAQKIELREWTDSAVRRFIQRVGIENIDELFKLRMADAQSNA
                     KTSFNPEEITMLQKRISEVLHQDMALKITDLKIKGEDLVAIGVKEGPQLGSILRELLD
                     MVIDDPMLNTKEKLLGKAKQLAGI"
     gene            20457..20720
                     /locus_tag="PKI16_02280"
     CDS             20457..20720
                     /locus_tag="PKI16_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HNR53277.1"
                     /translation="MRRIEKKTWPYLFEKVLSGEKQFDLRIAEFECEVGDILVLREWD
                     PEKEEYTGRELEKEITFVVKTKDLKFWSKEDINELGFLVMSLK"
     gene            20734..20937
                     /locus_tag="PKI16_02285"
     CDS             20734..20937
                     /locus_tag="PKI16_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53278.1"
                     /translation="MPNLYDEYIKAVSKNIILNGLNPADRLDLYCLLVAKDYAGVYSF
                     LKKKIPDLYERISANIKTVFLEE"
     gene            20967..>22319
                     /locus_tag="PKI16_02290"
     CDS             20967..>22319
                     /locus_tag="PKI16_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53279.1"
                     /translation="MTEIDNTEIGASSVQEPIQEEIEALNSSSAEIIEKDVSKINHKT
                     IQDRFDEYLDGVEITSRLRRIVSVAGIASTLGASLAGCKSNPDLHVERNVSTKVPMVE
                     AITIPEEIKTPPAIVHSLEREKIVYKDMDLSEIERWEELAPEKIEKTEKIIQTTEETE
                     IILIPVQQDYFYNHKENPTSWKLPAGLNLEIKDIFYFRGENDKIVRFSILPNVMASRS
                     VPIIFLDNGHMEGENYVVDGKNYKEQKSAKILSSFSYLAGENEVYPNKTINILTAMKG
                     IAERQEKDGKFLKGQEYSYLDLINLENLSKFEDGFTSSRAIVKGGGVCAGATLLSNSL
                     YELVLKENVSWNSTVKERWLHHPSTDVLGPFSVHDFVTDAAVEISNSPGGSDYDFKWV
                     QPKDAYIKIDIAMIPNGLRTQDTDSSGIDEPSDVEFMLTLSFTEIDPGNQSQKIEDFI
                     S"
BASE COUNT         7564 a         3285 c         4642 g         6828 t
ORIGIN      
        1 cgtagaaaag tacgaagaag aactcattag agttatcata cacggtgtcc tccatctact
       61 tggatttgaa cacaagacaa aactagatga catatctaaa caaaaagaag atatgtttgt
      121 aaaacaagag gagatattgg agaatatact tagagattta aagtaaactt cgataatatg
      181 aaattaattg taggcttggg taacccaggc aaagaatatt caaattccag acataactct
      241 ggcttcatat ttttggaagc tcttaaagac aaatttcttt accaaaaaga catatatgct
      301 actgagtgga ggatagaaga taccttccaa tctgaaatat cctttgttaa aagaggctct
      361 accattgtag ctgtgctggt caaacccatg acctttatga acaaatctgg ggaagctgta
      421 agtaaaattg ccaagaagct agagatagat gatatctatg aagatatggt acttgtacac
      481 gacgacttag atatcgctct tggagaattt aagatacaga ttggaaaatc tccaaatggg
      541 cataatggag ttaaaagtgt agaagacaga cttgggacga acaatttcaa gagggtaaga
      601 ataggggtag agaacagaca ggagaaaagt attaagggtg aggattatgt actaatgaaa
      661 tttacaaaag aagagaaagt aatgttagat gaaaccatac agaaagccat acaaggaatc
      721 cttgtagaca ttatttggta accattactc tggtatattc actttcaaac aactcttgta
      781 gcgtatgtgt tgttagtttc gtatcttgtg gtagcacaac accaacacta tcttcatcta
      841 caaccaaccc aatattcaca actgagtcat cctcatctag gaggaatatt aaggtcccaa
      901 tgttaagaac actctcttgg gttacatcta actccgttag caatgttttg gagctaaaat
      961 agccatacag gagctcagga tagcttaatt tagtccagta ctctctctct tctacagtat
     1021 ttctcaaagc tccttcaatc ggagacgaaa tattgaaata tgcttgagaa agtaacctgt
     1081 atgaattaaa tcctccaaac atatattgga tagaattggt atcaaaagat gctaacttcc
     1141 cactctcaga gatatcctta acctcttttg ttaacttctt aagatcaata tccacgatat
     1201 tatctatgcc atcgttatcg atatccagat cctttttgtc gtttagtcca tccatatcca
     1261 catcgtagtt ctctttgcct ttactgtcat acatcttact ttggttgtag gtttgggtgc
     1321 tgaagaaaac ggttacaaaa agaaatattg tagaaacaga aaggaagaaa tattttaaga
     1381 catcaaagaa aatattcttt tggaaaaatt tcttgtaaat atgcacaaag aagagtgaac
     1441 agagaaccaa ttctacggca aaatctggaa gtaggagata ttgctgaaat aggcttggtg
     1501 tcattaaaga tgagagtata ctgaatttga atgtagatat tggatatatc cacatgattc
     1561 ctccggttaa tgtatctgca atgaaatgta ccatcgtagc tatgaggaag gtatttaaga
     1621 ttacatccat taaatcatgg tgaagtacgg ctgaagttct tttgctggtt attttgtcaa
     1681 atatggctac tttgagttta agtattaacc atatccctat gtagaataga ggagcgtggg
     1741 taaagtagtt atgatgggaa aactgtggaa ttgaaaacat cggaaggatg agcagatcga
     1801 tatctgggag aataccaaaa agtattgcca atatgctaac aagtatctgt tcgtggaatt
     1861 ttaaattaga aatctttttc ttctgaataa tctcatttgc tagatacgat actgggccgt
     1921 gtgctaaaaa catatttatc aaatatttat attatctttt caattataca tattctccga
     1981 tgattgataa aatatggcgt ggtaaatatt acaaaagagt taagtaaaaa tttaggagag
     2041 ataaaagagg gggttttaaa gagtaaaaaa gtctctggca tagaaccata tctcttggaa
     2101 tttgctgccg aacttcttgg aaatatatta aacaagaaaa tattcctccg agacagtggt
     2161 gaaaatctac aaaagaaact tctcacgatt aaaaagggag atatgtttag tatttccaat
     2221 cttcttgact taggatatac ccgagtggag agagtttggt gtgaaggtga gatatctatc
     2281 cttggagatg ttgttgtcgt gtggccattt agtacaaaca atgtcctgcg aattagtctc
     2341 ttgggcgaaa agattgaaga gatggctgtt gtagattctc aaacaaggaa aaagattaaa
     2401 aatgtaaatg aaagggtatt tgtttccgag ggctcgaaga tatatctggg aaatgaagat
     2461 ggtgatagtg aggagattct agatattgtt ccaagagtga gcgaagaaga atatgtagat
     2521 ttagaactgc gaagtattcc tggcattgag aatttttcta atcctaaagt actgaaagag
     2581 attgtaaaaa atttccgaag caggaaattt aaaatatggt atgtcacaga caaaggagaa
     2641 cgagttgaga agttagtcca aaaggatgtc cttgaaatga tagatgatat ctttaccgaa
     2701 gaaagaaata tctccgcacg aggttttgta tctcaaagaa acgaggtagt agtactgaca
     2761 gatttggaag tacttggaga gatagatctt tctaattttg agaaagtaaa caaaagttta
     2821 gatccaaata gtatagaaat attgaaaaaa gttactcctg gggaatacct tgtccatgag
     2881 gatcatggaa ttggcaaatt tgtaggagtt attgaaagag agaaaggttt ttacattgaa
     2941 atatcttatg cagtggagga taaattgtat gtacctctta gtgcatcaga gaaattaaca
     3001 aaatatattg gagctggaaa aggcaaacca gttcttacag gtctaaacag tgggagctgg
     3061 aagagaattt ctcaaaaagc atctgagcat gcagaggaaa tagctaagga gcttctcatg
     3121 ttgtatgcaa tgagagaaac tgcacagtct gtatttactc tagaagatga agatagggaa
     3181 agctttaaaa ctttcgttga gcagtttgaa tacacagata cagatgacca gatactagca
     3241 actgaccaga ttgaacaaga tttcaaaaga ggaagaccta tggacagact gcttgtaggg
     3301 gatgtcggtt ttggaaagac agagatagct atgagggcaa tgtttccggt agtaaacagt
     3361 gggtttcagg tagcatttct tgcacctaca acaattcttg tacagcaaca cttaacagtt
     3421 ctaagggaga gatttaagaa ctacccattt aatatccaga gtctttcaag gttttcaacg
     3481 gaagcagaca aaaagagagt tttgcatgga ttaaaaaatg gtgtagtaga cattgtagtt
     3541 ggtacacatt ctctcctttc tgatgacatt caatttaaaa acctgggcct tctcgtagtg
     3601 gatgaagagc agaaatttgg tgttaaacag aaagaaaaga ttaaagggat gaggatcaac
     3661 agtaatgtac tttctttaac tgcaaccccg attccaagaa ctcttaatat ggctctcatg
     3721 ggtgtgcgag atatctctgt cttggcagta cctccaagtg gaagaaagga tattgtgaac
     3781 agttttgaac attttaattg ggatagtata gttaaggcaa tccaaaagga aatggataga
     3841 ggaggtcagg tatatttctt gcacaacagg ataggtaata ttctttcgat tcaaaagaag
     3901 ttacaagaac tgttcccaaa agctgtggta gatgtagtac atgggcagat gggaagtaaa
     3961 tatctctctg agcgaatgtc ggattttgta tctaaaaaaa cagatattct tctgtgtact
     4021 acgattattg aaaatggttt ggacatacca aatgcaaaca ctcttatagt tgatgatgcc
     4081 tcaaaattag gactttcgca aatgtaccaa atccgaggaa gagtaggaag atcccttgag
     4141 caggcatatg catatttctt ctttgatacc ttaagtgaga acgctatctt aaggcttgat
     4201 gctttgcggg attctcaaag tttaggttca ggattcttgc tttcaaacag agatttggaa
     4261 attagaggag caggagatat tctgggaaag aatcaatcag gaactatcaa ctcagttggg
     4321 tacggtttgt atacacaaat gctttcagag gcaattgaaa aaataaaagc aagggggtag
     4381 gagataatcc tttcgtagtg agccttcttt gagcaaatat ttttcacttg tgtacctata
     4441 tatatttact ctagtatata ccatgtcttc agtcctctac cgaaaatacc gtgcccaaga
     4501 ttttgaccag ctcatagggc agggacatat tactaaattg cttaaaaatg cagttaaaac
     4561 aaaccagata tctcaagcat acctttttgt tggctctcgt ggtactggga agaccagtgc
     4621 agcaagaata ctcgcaaaag cagtaaattg tctccatcca aaagaagatg gaaatccctg
     4681 taatgagtgt gaggtatgtg ttggtataac tacaggtaga ttgatggatt taatagagat
     4741 agatgcagca tcaaacagag gaattgacca gattagagaa ttaaaggaga agatagagta
     4801 ttcaccaaca gacagtaagt acaaggttta tgtcatagat gaagtacata tgctcaccac
     4861 agaggcgttc aatgctcttt taaagacact tgaggagcct cctgcccatg ttatattcat
     4921 tcttgcgact acagatgtac acaagcttcc tcctacgatt ctttcgagat gccagagata
     4981 tgactttaga cttggtacaa acgaggagat agagaaactt ctaaaggatg ttgcagtgaa
     5041 ggaagacata aagattgcaa aagaagcaat agatatactt gtccaaaatg ccaagggtag
     5101 ttacagagat gctctttctc ttctggatgt tgtctacagt ggaagaggag agggaattaa
     5161 aaatatctcc gagagagatg taagagatat cctcggtctt ccagattttg acttggtaat
     5221 atctcttctg aaaaacttaa ttgaaggaag tggaaagaga tcactagagg taattgaaga
     5281 tttagagaag aagggtgtta atctagcaca atttacaact tatgcattgg aaatattgcg
     5341 gaatatactg gtagagaaga taaaaggaaa ggtctccaaa gagtatgctt ttgccaagga
     5401 tacagatttg aagactcttc acaaattaat taatctgttt ctggatgcag agaacaagat
     5461 taaatattct agtaaccaag ggctagtatt agaaatgata attccagaaa tgattaaggt
     5521 agatagtgag gaaatcaaga ttgtaaatat agatgtaaag aataagatac agacaacaga
     5581 agggcaaaag gagacaacgg aagaatctga ggaggaggaa gaagaagaca atgttacgga
     5641 tgaaagtatc tctgtggagg atgtaagagc aaggtgggaa aaatttgtaa atgaagttaa
     5701 accttttaat ggtcatctat atgcattcct aggtggtgct agggttatta gttttgatga
     5761 gaacactctt cttttggaag ttccgtttgc tttccacaaa gacagaatag agattccaag
     5821 aagtagggac ataatatcta caaccttcca taaggtatat ggtgtcaact gcaagatagt
     5881 gtgtaatgtt aataaggatg taaaaccaaa gagaaaatct gaagcagaat ttgtacttcg
     5941 gaatctgccc cctgctcctc ctaaaaaaga agcagcccga agtagaaata tgaagaaaga
     6001 ggtcgaagca atctttgagg gtatgtagtg tctaacttcc caaattgagg tataatatca
     6061 atatatattt aatattttca ttacattaca atgataaatc ccgttgaatt acttagaatg
     6121 caaaatgaag ctaaaaagat gcaaaagaaa ctccgagaga gaagaattac aggtgaatcc
     6181 aaggatggat tagttaagct ctatatgaat gctgcacaac aattagaaga tcttcgtata
     6241 gatgaccaat tactttcacc agatatgtca gatgagctta gaaagtactt taaagatgca
     6301 tttaaggatt accagaagaa gcttcagaag gagatggttg ctgatatgga cttaagtcaa
     6361 ttaaagaaca tgttaggata atatgtctgt acttccaaaa tccgtagaac aagttataga
     6421 tgagttcagt aaactaccag gaattggtcc taagtcagca tcaagaatgg tttatcattt
     6481 tttaagatct ccaaacaaag atgctactaa attggcgaaa gctttgactc agatggattt
     6541 ggaagtaaaa aattgtagta aatgttttaa cgtttcagat aaagatatct gtgatatctg
     6601 ttccagtaag ataagaaaaa caaacaaact gtgtgtagta gaggagcctt tagatgttgt
     6661 ggcttttgag aattccgcgg tatatgatgg tttttacttt gttttaggtg gagttatatc
     6721 tcctgcagat ggtatagggg cagatgagat tagatttaca gaattaaagg atagggttga
     6781 ggaacttttg aaggagaatg atactgtaga agtaattgta gcaacaaacc ctaatctaga
     6841 gggagaggct acatccaact atattttaga tttgtttaag aatttactaa aagagggtaa
     6901 aataaagata tctaggttag caatgggtct cccaacaggt gctgatttag aatatgcaga
     6961 tagattaaca ttaaagagag cgctggaagg aaggagagat atttgatgaa actttacagt
     7021 acactagata aaaaagtagt agatattatc cccataaaag aagggcacat ttccatgtat
     7081 aactgtggac ccacagtcta ctatcgtatg catcttggaa acattcgtgc atatgtaagt
     7141 tgggatattc ttcacagagc attacggtat cttgggtatg atgtaaaaag agttatgaat
     7201 tttacagatg taggacatat gacccacgat gaagattttg gggaagacaa gatggatata
     7261 gaagcaaaga aagaagggct acaagcaata gatatcgcaa ataaatatat aaatactgtc
     7321 ttagaagact ttaaagcttt gaatatcctt actccaaatg gagaaataat tgaagagaat
     7381 ttggatttta aagatgtaga aaaacatggt tggatgaggg caacaacaca tattgctgaa
     7441 ataatagaaa ttatcaagaa gatggaatta aatggatata cgtatgaaac ggagaatgca
     7501 ctgtatttcg atgtaacaaa aattccggat tacacaattt ttacaggtca gaaactggaa
     7561 gataaaaagg tcggagatag agaagaggtt gttgtggata cagataaaaa gaatcctgca
     7621 gattttgtcc tgtggatgaa aaaagttggg aaatatgaaa accatgagat gttttgggat
     7681 tctccatggg ggtatggatt tcctggttgg cacatagagt gttctgcaat gggtacaaag
     7741 gccttgggag agaattttga cattcataca ggaggtgtag accatatccc tgtacaccat
     7801 acaaatgaaa gagcacaaaa tattggtgct tttgggcatg gtgtagttaa atattgggta
     7861 cacaatgaat gggtagtaaa caaagataat gcaaaattat caaagagcga taaaaatgct
     7921 gacacattgc cagaaatcgt agaaaaaggg tttgatcctt tagatattag atatctattt
     7981 gtatctgtca attaccatac caaattagct ttttctacag aagctctcga gggtgctaga
     8041 aatgcaagat taaatattag caagagagta aaagagttag gaactgaaaa tggaaatctt
     8101 ttggatggat atattcaaag atttagggat gctcttgaga acaatctaaa catgtctgag
     8161 gtattgtcca tattaaatga attacttaaa tcttccaata agaaagaaga tatccttgct
     8221 actgttttag agtttgacaa agtattggga ttaggtttag acaagattga agatactaag
     8281 atagatttag gaaaagaaat tgaggaatat gtaaagctaa gagatgaggc tagagcaaat
     8341 aaggactata ctaaagcaga tgaatacaga aaaaggatag aagatgcagg atatgtagtt
     8401 ttagatacgc cacagggtac taaatatcaa aagaaataga tgatacaaaa tactgtcact
     8461 atacaattag atgaaattca aagagagaat gcagaagatg tgctagaagc atatggatgg
     8521 aaagaggaat atgtaaataa tccatatgcc aagtttagaa tgaagagccc acaatcttct
     8581 aatgtaattc tttacactag cggtaaatta gtatttcaag ggagtgaaga ttttacaagt
     8641 atcataacgg cgataaagaa caacagagaa gatgaaagtg acattattcc ccatcttggt
     8701 gtagatgaag taggaaaggg agattacttt ggacctcttg ttgtagttgc atgttttttg
     8761 gatgaagaat ttttagaaaa aatctcgagt attggaatag gggattcaaa gaaattttcg
     8821 gactcaaaaa ttatggagat gtataacctt gtaaaagatc atccatactt ctacacttca
     8881 attgtatatc cgaaagagta tgcccagctc tacaaggaaa gtaagaatgc ctccatatta
     8941 cttgccagac aacattctaa agtcatagaa atgggactag gggacctcaa atcaaagaat
     9001 atagagtgta gtagggtagt catagaccaa ttctctagta gaaaatctag aatactcaat
     9061 gagctaggta gttacggtaa agaaattgaa ttaatacagt tccataaagg agagtcagat
     9121 atggcagtag catgtgcttc tgttatggca agaggaatat ttttagaaga gatggaaaag
     9181 atgagaaaga aatacaattt taatttccct aaaggagcat cagatgtagt agataaaggt
     9241 caggattttg ttaagaagtt tggtgctgaa gaattgggta atgtagcaaa gatatctttc
     9301 aaaacaactg ctaaagtttt acaaactaaa ctttagattt ttttctatct tctttcaaag
     9361 atttgaagag tgcaaatcca agcgtaagta ttatcacacc tgttattaag aagaagtagg
     9421 agctactctg ctgtatttcc tttgtgacac tattctcatc tataccattg tagaagagtc
     9481 ctaaaaccgt tctagggtat attaagtcgg atatatgcca ctcatctata acgattgtct
     9541 cctcgatgat gtcatttggg taaagctttt gtttatctcg atttattttg taatattttg
     9601 gagcatattt ccctttattg ttgtaaacct gtatttctcc atctggtact aggacatagt
     9661 ttgttatatt ttggtatata tatttaactt tagtatctct aataaacggt atacctctat
     9721 ctacaatttc catactaatc tgagcaaatt cagaggttat accttttaca tctccttgtt
     9781 caacaacatg tagcactact agatgagaaa ggttgtctac tgcaccaacg gaattttgtg
     9841 gattaatgaa accagcatcg tttgtttgtt taaggataat tagattaaaa tatgttccaa
     9901 gtggaagatt agaaggtact acaatttggt aacttaaact aatggtagca ttaggttcaa
     9961 ctttaaatgt gtctttatct gtcttaacaa acaagtatgt gttctctgtt ataatgctac
    10021 tagtcttagg attgtatcct acaacggtag gaaggatata tacctcttta tttgttgtat
    10081 ttgtaaagag gattttagag tcaaaagtat ctcctgaact tagcatggat tgggttatag
    10141 caggtgcaag agaattagca ttaacatgag ttaatggaag gaaaaacagt atggaaagaa
    10201 atccaattgt aaatattttt ttgattttca ccttgcttac ttaggtagtg gtttatactc
    10261 tgctttcttt ttcttagcag tttttacaat tatgacaagt agtgtgatga ttagcaaggc
    10321 aacaactata tatctccatg gaagtaccca gaaggaggtt tctgcagtaa gaggagcaaa
    10381 gcctggttgg aatgtacggt atgtcgcaac gagttttgca tctattggtc ctagcacaat
    10441 ctgtttgtca tttgttaaga attttccaaa gtcccatgta ctttcataat tacctgtatt
    10501 ttccctcata aggctttgtg tgttttcatt gaatggtatc cttgctatct ctttgtcaaa
    10561 tatattggtt aatacaattt ctccaatagg ggagatgtgc gtatccccaa gatttttgat
    10621 tctcgttgta aataccacat taggaaattc atagaagttt ttatctgtag tgaagctaag
    10681 cagttcagca ttctcgacaa aatccttccc gatttttatg aggattggag tgccagaggc
    10741 tagggtagat actgttcctg ttccaatgat ttgttggttt tctgtctgag atttaaatgc
    10801 aattattaca ttgtattctc cttttgtagc gtctttcggt actacaatat cgtaatatat
    10861 tttttcattt ctgtttggaa caagttctat tactttgcga tcaactttaa cccaagaagc
    10921 agcagagtag aggttttttg cttcttctgc ttctgagagg aagattgctg ttcctggttg
    10981 gttttctatt tgtcggaaac cagttacgag tacatcgtat gttgtagttg tatctgagag
    11041 gttccaaata actatttcgc catggtactt gtctcctggt tggagattta aggtttcaga
    11101 agtgggtgct gtacctatag ataggggtgc agcatatatg tttaaaggat tgaaaagaca
    11161 aaaagaaaag agtgaaaaaa atactgatac tacaaaatat attatgtttt tagagtatgg
    11221 ggcagcatct ttatttagtt ttttcatttc tagtctagta atatcctcca cagacataga
    11281 ttacactctc ctggtaggct cctgcaactg cagttgcacc agctctagct ccatatgtaa
    11341 ctgttgatgt ttcatttgat ggttgtgcag cattctggta aagaatcatt ctagctccgg
    11401 ctggtaatgg gtctaatgct ccataaaccc ctggtgtggc atttgagaaa tttgtggcaa
    11461 cagtagcttc ggatgctcca tcaggatctg agagtgctat accgaaacct tcgcttccgg
    11521 cagtggtaag attaactgtt gttgaaccag ttgaagcgat taaatatgtt gttggtgtgg
    11581 atttatacaa accagcatct gttccatttc ctgttccgta tgctgcccaa tagtatccag
    11641 cagtagttcc agaagtatcg gttccaattg tatgtgtact tgctgtagta tatgaacctc
    11701 ctgggtatag tgttccaaga tttactgtat ttgcacttat agtgcagttt acagaaggtg
    11761 ttgcagaaac ggtagcggat acaacgactg tatcatttgc aattaaggat acttttatag
    11821 tacttgaatc aattgttgaa ccactttttg cttctacggt tatctcatgt gctccggttg
    11881 tatcgtctgt accaataaca cctgcggttg ttccatttga tattttaaca ccatatgtag
    11941 ttcctgcagt caaacttccg acatttgata tcgttatagt atctacagaa cttccaccac
    12001 ttccttgtgt acatgctgcc gcaagagtgc cagaggttgg aagtacttca tcaatatccc
    12061 agtcagtagc agctaagtca gcggcagaag cagcaacccc agcaacagtt aatgttccag
    12121 ctgttctaca ccacatagca tcatctgcat ctgggaaata caaagtgacc gttccaccag
    12181 ttggtattgt tgttgttgta ttaattgcta aaacaatttc tagagtttct gcacttcctg
    12241 taacatttgc ttctaatcta cttaggtata catataaatc agagagggaa gcagctttag
    12301 attgaaagga tagggtagat acaaagaagg taacgagaga agttattaga gcaaatagta
    12361 atacggattt aagagctcta aactttttca tatttggcag ttaaaataaa taggtttctt
    12421 atacactcta gattacatta ataaatcctt ctatgtcaag tgataaccta gcagcctcta
    12481 gtaaagggga acaagtataa aatatatctc ttcagagtaa tccgaagcag gagttattcc
    12541 tgtactagct ctcgctttga gatatgctcc agttcttccg gctttgatag gaccgtctcc
    12601 agaatatatc ttgttggcag tggtagcaac tcctccgaca ctatttatac ttccagagta
    12661 gtctgttgta gctgtaagtt caccgtagta gggagaggag tcgtcgtaat ctatatagta
    12721 actttgaagt ccgaaccctt cagctcctgt tacatctaaa tcttctgtgc ttgatgttat
    12781 ggtttgtgta gtagttgcgc tgtggagtcc tccgtatttt ccaaagacaa taacagatac
    12841 tcctccagta gcactactgt ctatatctag ccaagcgaga cttgttgctg tggtcgctgc
    12901 agcaccggct atcaatgctg taccactaga aaaacctact gagtagggtg gagcactttc
    12961 tgtgtctata ccacttgcaa ttgcaatatc tatatcaaag gaaatgcttc ctgctgatgt
    13021 agtagtattg gcagcaatac tgtaatctga ttctgtaaaa tctccgtgta gggcagttag
    13081 tctaatgtag tactgagtgt ttgcagatag cccttgtata ttgaacacct ctgtttccca
    13141 tatagattct gttctgtagt cattaatatt atggtttgca taactttcag gtctaaatgt
    13201 agaaccatct atatattgta tatcacctac gaaattgtcc gttgatatct gtacaccata
    13261 tagggtatct gaagggttgg agcttgcaga tatttcaaat cttgctttgt cgtaacagcc
    13321 cccagttcca catatggcta ccatacctcc atctagtaat tcctgtggtc cagatgtaca
    13381 tgctgtagaa ccatcggttg tagtctcgaa gcattgtagt gtaggttgtg ctgctagaaa
    13441 tgctgcagat ggatcttggt aaagtctgta attagtggag taatttctag gatctgcaga
    13501 tacctttcca agactactaa ttacactgta gtttgtagag tctccaagcc cacctcctgt
    13561 tgcagttttt gctcccacta ttttgtaatt agtagaatct aaatcttctg caaaaacata
    13621 tacagtagaa ctaagtacta ttattaatag tagagatact gttttgaaaa tattggcaga
    13681 tttcatgctt taatgatatt gtaaattagt ttaattagtc aattttgtat gaaagaagca
    13741 gttgtatctg taaaaaatct cagtaaaaca ttcagaatat ttaaaagtaa gggaatttca
    13801 agcctgttta atagaaaata cgaaaatatt aaggctgtag acgatatctc ttttgaagtg
    13861 tacaaggaag aatttgtagg cttcataggt cagaatggtg ctggtaagac aacaacacta
    13921 aagtgtctaa ctggtttatt gactgcagat agtggagagg tagaagttct agggtttaat
    13981 cccaagaaaa ggaagcatga atttctttca agaatttctt tggttatggg taataaatct
    14041 cagctgtggt gggagttgcc agctattgaa actttcaaat taaacaaaga gatttacgaa
    14101 gtaagtgatg aaaagtttga agagattgtt ttagaaatgg tgacgctttt ggggatagaa
    14161 gaagtggtaa acattccggt tagaaaactt tctcttggag agcgtatgaa gtgtgagata
    14221 gtggtgtccc ttatacatac tccagagctt cttttcttgg atgagcctac attgggtttg
    14281 gatgtagtgt ctcagcagag actgagagat ttcttaagag agtacaacaa gagatacaag
    14341 gcaagtatcg ttcttactag ccataatatg gaggatatta aggatttgtg caagagggta
    14401 ataataatag ataagggtaa gaagatttat gatggtaagg tagatgattt agctaggaat
    14461 tttgtaaaga gtaaagagat tgttttttct ctttcttcag tagctacaca taaggagata
    14521 gagaaggttg gtaagataat agagttctct ggtttatcgg gaataatatc tgtaccaaga
    14581 gagagtgtag catctgcttc ttcgaagtta ctcaatactt tccaagtaga ggatttaaat
    14641 atacaggaac caagtttaga atatattgtt aaggaattgt ttgaggggag atataaggtt
    14701 tagattaaat atctgataaa aaagtggaca agattttctt gggtagtata taatgtaaat
    14761 acaaagatct agtgtccgaa ctgttaccgt atggtatgcg tttctgaggt tcctcagaat
    14821 aggcactaga tcgaactatt atccaaagat ccattcatct tctatcctct tttttattac
    14881 ttctttatat attttgctat catatttttc ctcttgatat gatctgtgaa tacttccggc
    14941 gaccatgtct gccaattgaa tcaatacact gttttttgaa tccacgaatt taagattatt
    15001 cataacttta ttccttattc ttccattcaa actttgtctc agataacttc ccattgcttt
    15061 tttgaatttt ctttcaccaa aaccgtcaag tctcaactta gcgtctttta tagttgcatt
    15121 gttatgttct aaaacttgtc gcaggaaaaa gttgtaataa atctttgtgt cggttcttag
    15181 gttggttgaa tatacttttt ctttccttac tacaattgct cggactctaa atttgcaagt
    15241 tttaataatt tctaggaaat ctaatttgtc ggatttcttt agattattaa atttgatttc
    15301 gtaatttaat ggtttcccaa gggctacctt aaagtctttt atttttatgg ctgttttttc
    15361 tgcctcaagt tcgtcatcaa agatgactag agcaataaca aaaaacttac tagaacctct
    15421 ttgagtttta aaacctgcat ctccagattc gtcaatgaag ataatcattc aactatttta
    15481 catcaaagag gatcgtttgt tttatgtgtt cagtatgatt taattaactt ttctaatttt
    15541 catgctacaa taagccatgc taactattac ctactttgtt catggtacaa ctatagataa
    15601 tgaacaacac atatctagcg gttggaaaga tgtagatata tctaaaaagg ggattcaaca
    15661 gtcaaaagaa ttaccattat taaataccac taagtttgat actgtattct gttctgatct
    15721 attaagagct atacattcaa cagaactatc gtttaaagat aaatatccaa tagttataga
    15781 taaaagatta agagaatgta actacggtga ttacaatgga aaaccatcgg atattgtaga
    15841 accattacaa gaacaaatga tatatacaag atttcccaat ggagaatcat acgaagatgt
    15901 aaaagagagg atagggttat tcttaaacga tattcaacat aaatacaatg ggaagcatat
    15961 agccatagtt gcacataaag caccacaatt agcattagat gtattactaa agaatatgac
    16021 atgggaagaa gcatttaaaa acgattggag aaagacaggg aagtgggaag caggatgggt
    16081 gtatactctt taatattaag ttctttaagt aaagtaattc ttaaatatct gatataatct
    16141 ctatgttttg gagaggtgtc agagtggtcg aatgagacgg tcttgaaaac cgttgtacag
    16201 gtttctgtac cgtgggttcg aatcccaccc tctccgtttg aatacaaata tatgggacaa
    16261 gatacaaaaa cagttgaaaa tatctacaaa ccaaaagaca gagatggagg ctttccttct
    16321 ggtatgtcat acttcaagaa aagaatctat gagataattc caggtctatt tgtttgggta
    16381 cttttacttc ttcctttaat ttttgcaata tttaaatggg aggaagcatt agttatatat
    16441 atcgcatatt tagttacata ttgggcatac aggactgtaa aatttgttgt aggaggatac
    16501 ataggtgtta gaaggatgaa gagagatata tccacggatt ggatgaaaaa gattaaagaa
    16561 gtaaacggag caagattcaa tgaattaaga tatgtgtacc tctgtcctgt gtattccgaa
    16621 agtctggaca ttttaattcc atcttttgaa agttgggcta atagtgatgt aggtgcaaag
    16681 aaaatagatg tggtgatggc aattgaggag cggaaaaaag acttacagat tgaaaacttc
    16741 aagaaattga aagaaagatt tggaaagaaa tttggtagta tgcagtatta tgtccatcct
    16801 gcggatatac ctggggagat agctggagta aagggggcaa atattaattg ggctgcaaga
    16861 cattttgtaa aagatttgga aaaagaaggg aaaaatattg gcaactatat gttagctaca
    16921 tgtgattctg accagagggt acatcctaaa tatctttctg caatcacata caaatacctt
    16981 acggttccaa acccagatct aggatactac gctacagcag tacatacatt taacaacaat
    17041 atatggaatg ttccatccat aattagaact cagtcaaata tgttgacttt agtaattctt
    17101 catctttggg tcttgacaaa aagcttaaaa gttccattta gtagggaatc tataaatgta
    17161 agagagacat tttcttccta tgttgttaac cttaaaactt taaaagaaat ggaatattgg
    17221 gatccagaaa ttccaaatga tgatactgca tttttctgga atactatggt tagatccaaa
    17281 ggtttagcaa aaagtcacga ggtatatata cctacatata atgatgcagt cgaaaatgag
    17341 acatatttta aatcccatgt ttctttttac aaacaacagt acagatgggg atggggtata
    17401 tttactctcc caatctccat ggcggcactt tctcaagaca aagagaattt tccagcacac
    17461 agaaaatttg ctcttttaaa aaccatgttc gaatatttat ggtttttaac agtagttttt
    17521 gttttaacat ttggtctttc tatcatggga tgggttaatc ctggctttca atatactgga
    17581 tttgcatata atctgcctcg tattctcagt tatatattca ctgccattat gctctcgaat
    17641 attgctgtag tcatatacag aagacagatt actcctgtgc caaaaggttg gaaatggtgg
    17701 agacatcttc tagacttctt ggaaacatat ctaatagcat ttaatatgct tactttctct
    17761 tttatccctt atatccaagc gatgaccgaa atgatgtttg gtaaggggag atttaaaaga
    17821 aatttctatg taacagagaa agtaaaaatt aaagagaagc gttaagagta ttttcatttt
    17881 gtttttctaa tatatggaga ataatatttt aattctccaa tatgtttgtt ctattatttc
    17941 tctttctttt ttcttctccc atatttgctg atgatttcaa tatcctagga gattcagggc
    18001 ctaacgaatt tttccaagca acagccctta ctacagagag ttttccagtg cagggaaaaa
    18061 tatcttttga tgaaaacgaa cttcttgggc agaacacgac aaatattcta aatacacaaa
    18121 gtatttttac acttgcaaat atggggtata caagaagaat ccccaatatt gcagataaag
    18181 tgtatggtcc tccaggaact aagatagacc tattgtattt ctaccagaac aacacaggcc
    18241 atagtgtaaa tattagtgaa atagctgttc ctttgagcag aagtattgtt tcaaatggag
    18301 atatatctag aactctaaat atagtaggag tagaaagaaa tgtagaattt ttgttttcag
    18361 gaaataatct ttccagagga ggtaaatatg tgtcttatga tggtttggga gaaatagcaa
    18421 atggagtcgg tggaggtatc cttctggata gttggtacat acaggatcct ttgacagtag
    18481 aacttttgaa ggcagaaaga gatttagaca tagttaattt aaaagttaag gtaaaaaata
    18541 ttacagaaga tgaatatttt actaatttga atttcaaaca taaagaatat caggaggttt
    18601 ttgctcttct cccttccgaa gagagatatt tagaatactc tttaaagaat gaagaaaaaa
    18661 tactggagcc atttacgctt acaaatccaa atggaaaaga aagatgtgct gtgttaggag
    18721 gtaactatta ccaatggtat tttaccgatt caatatctgt ctttgccaaa cgagaaaatg
    18781 acaagtgggt aggtggaggg tatgtacaac cagctgtcga gagtatttgt gtaaaaagaa
    18841 taggttatga attgaagtct gaagagattc aattagagtc tcgagagata ctgggtgtag
    18901 cagatgattt aaaggaactt cctcaaacaa acagttcatc tttcctttgt ttgttactat
    18961 ttgttgttct agatgtcttt ctttggtatg cttttcttag aaggaaaaag aaatatgaag
    19021 ataaaaatag taataccaga ttatgttcaa aaagtaggta gaatgcttgg taaggaggga
    19081 tttgcttgct acattgtagg tggggccata cgagatctag ttatgggaaa gattccacat
    19141 gactatgatt tagcaacaga tgcacttcca gatgagatgt taaatatctt tcctaagtcg
    19201 gtatcaattg gtgcaaaatt tggaaccatt atggcacttg tacaagatgg caatggagaa
    19261 actcatgagg tagaggttac aacttttaga agtgagtcta actatgtaga tggtagatgg
    19321 ccagcaagtg ttaagtttgt tgaagatatt gacctagatt taggaagaag agattttaca
    19381 tttaatgcta tggccataaa tcttgcagat gtagaactag atggaatgga aattgaaaag
    19441 gaagtagaga tatatgatcc atttaatgga cttggagata taaagggaac attaattagg
    19501 gcagtaggga ctcctttgga aagattcaaa gaggatggtt tgcgaggttt taaggcatgt
    19561 agattggcgg ctcaattaaa ttttgagata gatgaggaaa cattcaaagc aattaaagaa
    19621 tctcttccta tagcttctca ggtttcgatg gagagaatac gagatgagtt tatgaagatg
    19681 cttctaaatt cgtccaaacc atctattgga atagaactaa tgagacagac tggtcttcta
    19741 aatatattta tgcccgaact tctagaaggt gtaggtgtag aacagaagta ttaccatagt
    19801 gacgatgtgt actggcacag tcttaggaca tgtgacagtg cagaggattc tgtaaaactg
    19861 gcagctcttc tccatgatat tggtaaacca aggacgaata tgggtaatgg tcatttctat
    19921 ggtcatgatt ctgttggtgc ggacatggca gaggtaataa tgaagagaat gaaatttcct
    19981 caaagtgaga taaagagagt tcttatttta atcaaaaacc atatgttcta ctatccatat
    20041 gtgacagaag aaatgtctga agaagatgca cagaagatag agttgcgtga gtggacagat
    20101 agcgccgtaa gaagattcat acagagagta gggatagaga atattgacga actcttcaag
    20161 cttcgtatgg cggatgctca gtcaaatgca aaaacatctt ttaatccgga agagataacc
    20221 atgttacaaa agagaatttc cgaggtatta catcaagata tggcattgaa aatcacggat
    20281 ttaaaaataa agggagagga tttggttgct atcggagtta aggaaggtcc tcaacttggt
    20341 agtatattaa gggaattact agatatggta attgatgatc ctatgcttaa cactaaagag
    20401 aagcttctag ggaaagctaa acagttagca ggtatttaaa tttttaaaat attaatatgc
    20461 gtagaataga gaaaaagaca tggccatatc tttttgagaa agttctctca ggagagaagc
    20521 agttcgactt gcgtatcgca gagtttgaat gtgaagtagg ggatattcta gtattacgag
    20581 agtgggatcc tgagaaagaa gaatatacag gaagagaatt agagaaagaa attaccttcg
    20641 tagtaaaaac aaaggatttg aaattttgga gcaaagagga tataaatgag ttaggatttt
    20701 tggtaatgtc tttaaaataa ttgctacaaa tatatgccga acctatacga tgaatatata
    20761 aaagcagtaa gtaaaaatat aattctaaac ggattaaacc ctgcagatag attggatctc
    20821 tattgtttgc tagtagctaa agattatgca ggtgtatatt cttttctgaa gaaaaagatt
    20881 ccagatttat atgagagaat ttctgcaaat ataaaaactg tgtttcttga agagtagttc
    20941 aattaatgta aaagccttac tatcaaatga cagaaataga taacacagaa attggagcaa
    21001 gtagtgttca agagcctatt caagaggaga tagaagcact taatagttct tccgccgaaa
    21061 ttattgaaaa agatgtctct aaaataaacc ataaaaccat tcaggatcga ttcgatgaat
    21121 atcttgatgg ggtagaaatc acatcaagac taagaaggat agtgagtgta gctggaatag
    21181 ctagtacact gggagcatca ttagcaggat gtaagagtaa tcctgattta catgttgaaa
    21241 gaaatgtttc aacaaaagtt ccaatggttg aggctattac tatccctgaa gaaataaaaa
    21301 ctccaccagc tatagtccat tccttagaaa gggagaaaat tgtttacaaa gatatggact
    21361 taagcgagat agaaaggtgg gaagagttag ctcctgaaaa gattgaaaaa acagaaaaga
    21421 ttattcagac tacagaagaa acagaaatta ttctaatccc agttcaacaa gattattttt
    21481 ataatcacaa ggagaatcct acctcatgga aactaccagc aggcttgaat ttagagatta
    21541 aagatatctt ctactttaga ggagaaaatg acaagattgt tagattttct attttgccaa
    21601 atgtgatggc ttcaagaagt gtaccaatca tttttctaga taatggccat atggaaggag
    21661 aaaactatgt tgttgatgga aaaaattata aagaacaaaa atctgctaaa attctttcaa
    21721 gtttctccta tcttgctggt gaaaatgaag tgtatcctaa caagacaatc aatatcttga
    21781 ctgcaatgaa gggaattgca gaaagacaag agaaagatgg aaaatttctg aaagggcagg
    21841 aatattcata tttagattta ataaatttag aaaatttaag taaatttgaa gatggattta
    21901 caagtagtag agctattgtt aagggagggg gagtgtgtgc tggtgcaact cttttatcta
    21961 attcgttata tgagttagta ttaaaggaga atgtttcttg gaatagtact gttaaggaga
    22021 gatggcttca tcacccatca acggatgtct tggggccttt cagtgtacat gattttgtaa
    22081 cagatgctgc agttgaaata tcaaattccc caggaggaag tgattatgat tttaagtggg
    22141 ttcaacctaa agatgcatat attaaaatag atatcgctat gattccaaat gggctgagaa
    22201 cacaagatac ggattcgtct ggaatagatg agccatcaga tgtcgaattc atgctaacac
    22261 tttcttttac tgagatagat ccaggaaatc aatcacaaaa aattgaagac ttcatatct
//
LOCUS       DAOQWP010000013        21428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_2822_length_21428_cov_7.688345, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000013 DAOQWP010000000
VERSION     DAOQWP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 21428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21428
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2822_length_21428_cov_7.688345"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKI16_02295"
     CDS             complement(<1..574)
                     /locus_tag="PKI16_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR53280.1"
                     /translation="MSKKVKNKIEECERVPIVTILGHVDHGKTSILDKIRETDVQGCE
                     VGGITQKVSLFTIKVKDGKQITFVDTPGHEAFDLMRSRGGSIADIVLLVVAADDGVKP
                     QTKESIDIINKSSAKPIVVINKCDLPNIDLPKVKRDIVSNGLQIEELGGNVPLIEVSA
                     KTGKGIDELLDLILLVSEVDGLQEREDLPAG"
     gene            complement(593..1759)
                     /gene="nusA"
                     /locus_tag="PKI16_02300"
     CDS             complement(593..1759)
                     /gene="nusA"
                     /locus_tag="PKI16_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNR53281.1"
                     /translation="MAMTSEFVAAINQISAERGIEKEEIFKALESAILVAYKKEKYFD
                     YSLSEEEQEELGDNLSVELDRETGEFKLIATKEVVQKVKDDNKEISVSEAEMISPSVE
                     VGDSVQIEMPAEDLGRIAAQTAKQVILQKIRESEKDAVLGEFSDKVGEVFTALMQRMQ
                     KGQVIFEIGKATAVMPQEEQISNEFYRLGDRYKVLLKSIEDGDMIVSRADPAFLIELF
                     KLEVPEIESGVVEIKAVAREAGSRSKIAVVSHQEGVDPIGSCVGQRGIRIANVMNEIG
                     EEKIDIIEWDEELERFVANALSPAKIENVKIKEDTATVKVDDEQLSLAIGREGQNVRL
                     AWKLTGIKIDIVGSSGKTPEKKEKVEETEKVKEKEGKKEVKKAKKSKKEKKSEE"
     gene            complement(1879..2955)
                     /gene="dnaJ"
                     /locus_tag="PKI16_02305"
     CDS             complement(1879..2955)
                     /gene="dnaJ"
                     /locus_tag="PKI16_02305"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNR53282.1"
                     /translation="MAKRDYYEVLGVDKKATQEELKKAYRKLVKKYHPDVNKDGDAEE
                     KFREVQEAYEVLSDESKRSAYDQYGHAGTAGFNPGSTNGYDFGGAPFDMGDIFNTFFG
                     GSNDFGFNFSGFNEQSRVDRGADIRYRVRLSFDEAMRGGEYEIKITRDVECEVCNGSG
                     SADGKTEKCSVCGGSGQERKVRSTILGQIAVMSTCSKCHGTGKEIKNPCKSCRGSGVK
                     SMQENVKIKIPAGAYDGMVLKFGNGGNVGRDGVGDLYVEIEVEPSEKFERRGNDIYSE
                     ESIPVHRAVLGGDVQVDTILGEVKLRIPKGTQSGSIFRIKGKGCAVLNSENRRGDHYV
                     RIVVDIPSRLSGKEKELWEELSKV"
     gene            3046..3843
                     /locus_tag="PKI16_02310"
     CDS             3046..3843
                     /locus_tag="PKI16_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNR53283.1"
                     /translation="MTNEWNVPTKNEKKTKNVQLWISGILAFIGLVIVSTQLIPLTGS
                     FLDGLIEKLRTEFKIKPVPDSYKQYIEEQFAYYDPGKSYFANLAEQIGDIEIANQYSY
                     DPESGTQKEITVDTKYDKDMYISISSIGIKDIKISPNVESSDEKIYNKYLKSGVAHFK
                     GTPLPGDGGNSFIYGHSAVDSFFRNHKNLPETIFSRLNSIDIGQDVVITKDKETFTYV
                     VRNKKIVAPDDFSILRTQNNKETVTLMTCWPLGIGTKRLVVVAEREI"
     gene            3860..5188
                     /locus_tag="PKI16_02315"
     CDS             3860..5188
                     /locus_tag="PKI16_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNR53284.1"
                     /translation="MEFKNKKLIIIIAIVLFLLLAGTGTLLLLSKDNGNNNNTVKNVE
                     LVYWGLWEPSSVMQPLIDEYESTHPGVNILYSQQTFSNYESRLFTRLQQASSSTEPAP
                     DIFRIHNTWVPKYYSYLSPLPENVMTRDQYSQTFYPTALNDFTAKDGKLYAIPLEIDG
                     LVLFYNKQLLTDAGFSEPPKDWDSLIDLAHKLTKKDSSGKITQAGLAIGTSRNILHAS
                     EIFSFLLLQEGVELIDSTRTNVTLNTTKVGSILNFYTGFATGKQAVWSSDLRYDLEMF
                     YSGKLGMMIAPSWRAFDIILSSPTIEFGMATLPQLGANQEKIYYSTYWAEAINKSSEN
                     TDVAWDFVNFLAQKENMMTKFSNESKIRAFGEPYSLVELNSSMSGNSYLTAIAEMAPY
                     MKSWQMGDETIVKQTLNQLITSVVEDGQELNVALKNAEEDINDQLAQTNK"
     gene            5205..7142
                     /locus_tag="PKI16_02320"
     CDS             5205..7142
                     /locus_tag="PKI16_02320"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNR53285.1"
                     /translation="MQRIKAPRKSAQISVIYLTFFLLLSIPLVVFGVLNGNFDIRNRA
                     FDDLEVSEENPCIISLPNVNPYTLEVGRPITIQVDAKLSNAIISELNITDSSGNSIYT
                     EKFDNAPLEIATSFKFTPTKSGVVDLLGIIKKDGGGSVGCKISSPYDILGLRALANNS
                     APVFISQPSSSKPSQSIKTGTVYEYTLSAVDEDDDRVNYSYSFTPEAKWLKPTIIEDG
                     SSGKLTLKFTGSTNVPGSYLANVFIHDGYSMHLASQSWVISVSPAKNDIPIVKFISPV
                     SPMTVTQGKNITVSWEANDLNFITKYQLYITKNYTNTENWIPINTNIPFKTRNFNFST
                     SNLDTGTYKVILKAVDNQSPQVSGLAISPEIVITKSGQSTIPTNDIPIIDAPQVINMI
                     PLSTDKITNKRVTIKASLISAEKADIDEKTILFKLDSVDKTSEIKINKISTQEYTIIY
                     QPLEDLTEGIHKAEAYFKDSAGHEVTKEWTFTIQNSVEELKDETINIFGLDISKRILL
                     IVGIGLVVVILAIVAPIIIFNVWKEDKMRDQEEPHSNEPIQPFIQQQEPEYIEPPVQA
                     EEIKEKVEKQPVEVPVKEVKEPVIFAAPEPVIEEETTPAPTEILPPEPDLSTDLPTDD
                     TIKQLFEQVQKAQEEPPQTNS"
     gene            7172..8902
                     /locus_tag="PKI16_02325"
     CDS             7172..8902
                     /locus_tag="PKI16_02325"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006187823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNR53286.1"
                     /translation="MNHLSYKEIRKKYIKFFEQNNHKEINSAPLVPENDPSVLFVNAG
                     MFPLVPFLQGEKHPSGTRLVNSQRCVRTIDIDEVGDAYHCTTFEMLGNWSLNDYFKKE
                     ALSMTLKFFVEELGFDINKLSVTVFKGDNKIPRDTESIKIWESLFKKYNVELKGKITE
                     NGKEDNWWELPTGGPCGPDSEIFFNTGKENVEIGNNVFMEYLKSGNEYKDLGRHNVDF
                     GGGLDRLACICQEVESIYETDIYKPIFEKVKELSKKDILESQRIITDHIKAATWIIMD
                     GVIPSKTQQGYILRRLIRRAVRHGRKLEIEGRFTRDVAQVAINQFAPIWKELKNQEKY
                     ILDTIEEEEVKFSRTLQKGLAELDRMVSREEIDGKKAFILYETYGLPIEVIQEILTEK
                     NRKPVDINEFNEAEKSHRNLSRTASAGLFKGGLADTSEQSTKYHTATHLLLSALRKVL
                     GTHVYQRGSNITPERLRLDFPNEEKLTDEQIKEVEHIVNEQIRLKLPITFEEMDKQDA
                     LSIVPFAAFEEKYGDILKVYYIGPKDNPFSIEICNGPHVKNTSQLGEFKIVKQESIGS
                     GIKRIKAILI"
     gene            8899..9351
                     /gene="ruvX"
                     /locus_tag="PKI16_02330"
     CDS             8899..9351
                     /gene="ruvX"
                     /locus_tag="PKI16_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNR53287.1"
                     /translation="MTEYPVLAIDFGKKHFGIAISDSKGIIASPLEVISITQRRGIEY
                     ILERISNICEEYKIKTILLGMPQVFTEAHSKTPKAIIRFEKKLREVLDLPIILYDESF
                     STTSAQNMLTSSGSNTKSSRKKIDKVSAAVFLQEFLNSSKINNDKKHN"
     gene            9332..10333
                     /gene="mltG"
                     /locus_tag="PKI16_02335"
     CDS             9332..10333
                     /gene="mltG"
                     /locus_tag="PKI16_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNR53288.1"
                     /translation="MIKSTIRIFLFALVTVIIATIVIKLDYDKALRTPNSDSQEKVIV
                     QIDQGATVEQILTKLVDQGVIQEKWLTYVKLYLKQENLAAKLQAGTYELPKNLSIKEV
                     IDSLQNGKNPDIWVTIPEGLRKDEIADILNRELSKADTTNFSKEEFLRLTTDRAYIQT
                     LGLTVNISDLEGYLFPDRYAFPITTKTSDTIDKMVKNFITKVGTADTYDQIILASIVE
                     REGFSDQDRPIIAGIFIKRLEEGWLLQACATVLYPLKDWKHEITDADKKSDSPYNTYK
                     KPGLPPTPICNPGLESINAVRNYKKTDYYYYIHDNNAIVHYAKTLEEHNANINQYLR"
     gene            10347..11801
                     /locus_tag="PKI16_02340"
     CDS             10347..11801
                     /locus_tag="PKI16_02340"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNR53289.1"
                     /translation="MTIDIQLDEKKPAKIVTPELITKSEKPIKIESPRKKKKWSIFVL
                     VGIVLVILGGLVFLVYKGIAFSKNIGFNFNANDITAPEKEPELAKDSSGKYTNILIVG
                     VDTRDKEALLNTDTLILASYNYETKDIVMISTPRDFMAEINNSNWFNKINSVYASAEN
                     KTKGSGMMALRKTIEEVTGTEIQYHAMVDFKAFEKIIDAVGGVDINVENSFTDYKYPD
                     GYSYKTISFKAGPQTMDGKTALEYARSRHSQQNNEGGDYARAKRQQKVIVALQEKILN
                     TSTLTSPKAIMEIFASVANNIKVSEFTIDDIQAGLNLAKDFQDNNGKSYSFVLDPSAG
                     NSQIISNQNVFGPDGKTVTDYRIGPKLGLGNYTDINKYVKQILLHPQLYTENASVYVY
                     NVGLGAQEASKKVAELKKAYPYLKIIYSGTKYSNKEGSAIYSNTPDTYLRTVTEYSKF
                     LGITQTTKPDYITTNLNGGDVTILLGKPVQLSSE"
     gene            11794..12732
                     /gene="holA"
                     /locus_tag="PKI16_02345"
     CDS             11794..12732
                     /gene="holA"
                     /locus_tag="PKI16_02345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNR53290.1"
                     /translation="MNNKVNYKLYHGNSVYLSLRAVKKDIATNTEKKNLVTIEADTEK
                     AQKILDSIQNQGLFVSKRVFLIKRLYKNKEKDSLIEKLLSLLNDQFPDDIYFWEDQKI
                     RSTTKYLKFFKANNSLEEYSSLNKRNFLSWLNQELKENNLKADETTKRLLAQRTNYDP
                     ERCANEIKKHKLNDTEEIKEKDLDILTTDTLEEDIWKLVDAINSEKKERSVEILEKLY
                     SQNTDPNFILSMLARNLRLITMAKHLKKQKKSFAEICSIMKVAPFSLYPILGVIDRYD
                     MEKIKLLYTKLSNLDYQIKTGKIDGMLGLTLICPYL"
     gene            12799..14586
                     /locus_tag="PKI16_02350"
     CDS             12799..14586
                     /locus_tag="PKI16_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HNR53291.1"
                     /translation="MEEEIKVTTKKKTSKTVKILLIITGILGLALVAVAIYFYSIADT
                     SKDDEIKQTTCGCYYIDPSVVSECGDPKRGFIFETVTVPSTQTCKAACSTSKLSVNIL
                     NSTTEQDLYQICQLPSILDSRCTEMVIKDSTGKIVTGKVSANDVLSVEAKFNQEYIDY
                     QFTINNETKLPDTISTDKLSITKSIADLSGITTLNIVATGKTSTGEQINSPLCRRLIE
                     VAQDGVKNVSGIQIQTRTVDTKLKIAKIKINASNITDEAKVTLKFSFSNNFPTLSMKE
                     GFTANKTKGEIEIVEQDLYNSSNFTEVKSFSQLDNHKGELTITATVLENGKSLGSVSK
                     AITFPETSSNVENPDADTTEESNFTIAKTASEQCVERVAPNNFVTFTLTSTNRGTISQ
                     GITSVKDKLPLGFTYVANSSKINNVVVTDSNYVTVSTIGSTQEIVWTKTEPWIVNSNQ
                     ALTITFNAIAGGNALTGKNTNEVILTPQQIPADPSSLRATATLLVQQDCDNPEEEIPE
                     VPVNPDEDIPQTGIFDSVISKVILGILTITFGWYIYSKPSGRVLAEKLIETPAFKGAE
                     MTTWKIFKPKKYFEEKIINKISKVNQKKR"
     gene            complement(14561..14968)
                     /locus_tag="PKI16_02355"
     CDS             complement(14561..14968)
                     /locus_tag="PKI16_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53292.1"
                     /translation="MSKKIFMILGAVSFLVTILSSVMLFVKEANSIDLVGYIRKDCVP
                     YNVFVEKGEKEYSVKISWVTKAECVGFVLYGNSSNSLDLVAVDLTNKAKSKTHEVVLE
                     NILSSEHYYFLINSGEKSYGSNGSPVIFSSDSL"
     gene            complement(14961..15377)
                     /locus_tag="PKI16_02360"
     CDS             complement(14961..15377)
                     /locus_tag="PKI16_02360"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HNR53293.1"
                     /translation="MERSRKVKIFLSFLVFLLISGGAVFSFLNYSPDKDTNDEVLGNQ
                     DLYSVPRITSLAPISVYEGEEYIYMVKVTDNDTEMEDIAINLLEGPSWLGVSELKISG
                     TAPFGSVGTYRLVIRVSDGDNSSVQESYILVEAKDE"
     gene            15471..15767
                     /gene="rpsT"
                     /locus_tag="PKI16_02365"
     CDS             15471..15767
                     /gene="rpsT"
                     /locus_tag="PKI16_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNR53294.1"
                     /translation="MPNLKTSIKDLRQTKKREKVNDRFKNRIKKAVKKTDSLIKDGKD
                     TEAKITLKNVYKVLDKAAKKNVIKNKKADRIKSRLSKKLNKLTPNNVKTAKKGA"
     gene            15739..16287
                     /locus_tag="PKI16_02370"
     CDS             15739..16287
                     /locus_tag="PKI16_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53295.1"
                     /translation="MSKLLKRALKISVIPAILMIAGKFLGIFVTSVLYDLQFNIDNDI
                     SGIFSVQIYFTDPETTLFVNSMSNLTMILSLLLPILYFIIKTYLYTEAHNNPKTIIKM
                     TKLNLLNWITKKDTSFLKTFIWCAFLWLATAITITHSIQGNTYSWIAVTSGVIALLVT
                     LFTIKTFELETDKIYPIEKKYF"
     gene            16289..16861
                     /locus_tag="PKI16_02375"
     CDS             16289..16861
                     /locus_tag="PKI16_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53296.1"
                     /translation="MPSKLRSPQFKKVVGQILEPFALGLLALLFIIPTLTVFNLSPIT
                     KTFEKLNVLGVSSNEDVSFTLVEGKHEIIRSEYLVKNEEGDYTYSTTLNKRASDSYSK
                     PIIELKNNTQEQQTIVLYGQTKAPTRSNISVIINDHPYKLQDESNNTYPQEITIDPGK
                     STIMFLAIENLSGVQFSEEFEMHLTVKSDI"
     gene            16901..18712
                     /gene="lepA"
                     /locus_tag="PKI16_02380"
     CDS             16901..18712
                     /gene="lepA"
                     /locus_tag="PKI16_02380"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNR53297.1"
                     /translation="MLEKIRNFSIIAHIDHGKSTLADRILEATHTDLSHEKRKSRVLD
                     RLDLEQEKGITIKLQACNMVWKGYQLNLIDTPGHVDFSYEVSRSLAACEAAILLVDAS
                     QGIQAQTISNTKKALDLGLKLIPVLNKIDLDGIDIEEREKEITELLGFSKDEIIKVSG
                     KTGLNVDQLLDRIVEQVPPPVGDPNKPLKALIFDSFYDEHRGVVVAVRIFDGRIKHIH
                     GKPIELNILQRKQTFRPTEIGMFTPDLEETGELNTGEVGYIATGLKDISYFTVGDTIS
                     DREDTQPLAGYKTPKPNIFATFFPTDSDEYDNLKTGLNKLALNDASLTFMDQRSNLLG
                     SGFRCGFLGLLHMEIVQERLEREYDASLIITAPTVEYKVTTTAGEEISVQTPQELPDP
                     SGIKEIREPWVRTEILTPSKYMGDLMKLCQSRRGEYVNTKYLTTNTSELKMRDQYIAL
                     EYDIPLASLISNFFDQMKNLTSGYASMEYEFIDFFPADIVKVSIFVNHEEIPALSFLE
                     VRDEARSKATKLLAEMKRVIPRQQFPIPLQAAINSKIIAREDVSAFRKDVTAKLYGGD
                     YSRKKKLLEKQKKGKERLKKIGKVNIPQEAFLAILKT"
     gene            18712..19752
                     /gene="recF"
                     /locus_tag="PKI16_02385"
     CDS             18712..19752
                     /gene="recF"
                     /locus_tag="PKI16_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNR53298.1"
                     /translation="MITKIKLTNYRKFKDLELDITNNIVVLHGDNAKGKSTVLEAIHI
                     ITNGTSPWTSSDDYINTEQDENEKFSRVEIIEDEKTYSFMKDNSKRVFKIDNHTTTPK
                     KFFEQHASIIFNPEQIEILMISPSKRRDFLDEVISKINYEYSETLAQFKKVLRQRNAY
                     LKKLSKKFYEQGIIARNDTQLNFWTNEFVKLSKDIQKNREEISRELSFGKFHIDYIKS
                     NKRDSLENEIENSKKRDIATGYTNIGPHRDDWDIINGKNIKRFGSRGEKRLAIGQLLF
                     KVQDLINSELGYYPILLLDDISSELDNKNTKIIFDKKNIVKQQTFITIIKYDELPKNI
                     LKQAQLIDLNSI"
     gene            19885..20721
                     /locus_tag="PKI16_02390"
     CDS             19885..20721
                     /locus_tag="PKI16_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HNR53299.1"
                     /translation="MKKQIIQVKGEDIELIVKNNQDYISLTSIAKYKNTLHPDDIIKN
                     WLRNKNTLEFLGLWEKINNANFKPVEFDGFKSQAGTNTFVITPKQWIRNVSSIGIISK
                     PGKYGGGTYAHKDIAFEFASWISPEFKLYLIKEFQRLKEDESRRNSLDWNLTRTLAKV
                     NYKIHTRSIKENIVPQLVTKKQKTFVYANEADVLNVALFGLTAKEWRESNSEKEGNIR
                     DYADIRQLICLSNLESLNAELIRDGLSQKERLLKLNITAIFQMRALTGIEDPFCPLCA
                     LL"
     gene            complement(20713..>21428)
                     /locus_tag="PKI16_02395"
     CDS             complement(20713..>21428)
                     /locus_tag="PKI16_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53300.1"
                     /translation="MGASLKKDDIKRIWGKSFLRESKEKYEQNENEIAKGKLEYAENI
                     VKVLKIFNWVKFIGVSGSVGAGFAKEEDDIDVFVVVKDGTAWVCRGLISIANLFHNKI
                     RAKRHKVIKNKLCLNMICEERGLKFDNDIFNFHELMFLVPIYNEKYLNFIYSNNPWLR
                     ESYFVKKDLLYTKISRGKNANFFIKFLNTVFYLLQISFMYISNHKPDLKRIERNYKKG
                     RIEFFEEDFKKEKLEDYLK"
BASE COUNT         7916 a         4105 c         3278 g         6129 t
ORIGIN      
        1 ctcctgccgg tagatcttct ctttcttgta atccatctac ctcagagaca agaagaatta
       61 aatctaacaa ctcatctata ccttttcctg tttttgcaga aacttcgatt agtggaacat
      121 ttccaccaag ctcttcaatc tgaaggccgt tacttacaat atctcttttg acttttggta
      181 aatctatatt ggggagatca catttattta tgacaactat tggtttggca gaggatttat
      241 taataatatc tatggattct tttgtttgag gtttaacacc atcatctgcg gctaccacca
      301 aaaggactat gtctgcaata cttcctcctc gggacctcat caaatcgaaa gcttcgtgtc
      361 ctggagtatc tacaaaggtt atttgttttc catctttaac ttttattgta aaaagtgaga
      421 ccttttgtgt aattccacca acttcacatc cctgtacatc tgtttctctt atcttgtcca
      481 gtattgaagt ctttccatga tctacatgcc caagaattgt tacaattggt actctctcac
      541 actcttcaat tttattttta actttcttgg acatacaaac ctttccataa tattattctt
      601 ctgatttttt ctctttttta ctcttcttcg ctttctttac ttcctttttt ccctctttct
      661 cttttacttt ctctgtttct tcaacctttt cttttttctc tggagtttta ccagaagaac
      721 ccacaatgtc aattttaata cctgttaatt tccatgccaa tcttacattc tgaccctctc
      781 taccgatagc caaagataac tgttcatcat ctacttttac tgttgccgta tcttctttaa
      841 tctttacatt ctcaatcttt gctgggctta gcgcatttgc tacaaatctc tccagctctt
      901 catcccactc aatgatatct attttttcct ctccaatttc attcataaca ttagcgatac
      961 ggatacctct ttgtcctaca catgaaccaa ttggatctac accttcctga tgggatacta
     1021 ctgctatttt acttcttgaa cccgcttctc ttgctactgc ttttatttct actactccag
     1081 actctatctc tggtacttct agtttgaaaa gctctattaa gaaggcagga tctgctcttg
     1141 atacaatcat gtctccatcc tcgatacttt tcaaaagaac cttatatcta tcacccaatc
     1201 tgtagaattc attcgaaatt tgctcttctt gtggcataac tgcagttgct tttccaattt
     1261 caaatatgac ttgacctttt tgcattctct gcataagtgc tgtgaatact tctccaacct
     1321 tgtcactaaa ttctccaagt accgcatctt tctcagattc tctaatcttc tgaagtatga
     1381 cctgttttgc tgtttgagca gcaatccttc caagatcttc tgctggcatc tctatctgta
     1441 cagaatcccc cacttcaaca cttggtgaaa tcatttcagc ctcagataca gatatctcct
     1501 tgttatcatc tttaactttc tgtacaactt cttttgtagc tatgagttta aactctcctg
     1561 tctctctgtc taattctacg gaaagattat ctccaagttc ttcctgctct tcttcagaga
     1621 gtgaatagtc aaagtatttt tcttttttgt atgcaacaag aattgctgac tctaaagctt
     1681 taaatatttc ctctttttca atacctcttt ctgcagagat ttgatttatt gcagcaacaa
     1741 attctgatgt cattgccatg gtctaaatat attaaaatta atatgcttgt ttaataaaaa
     1801 aggtgagaat ttccatccca ccttctcttt gtataacttt cgggtttagt ataacacatt
     1861 ctaaactcaa aaccaaatct acactttaga cagctcttcc caaagctctt tctctttacc
     1921 agataaccta gatggtatat ctactactat tctaacataa tgatcacctc ttcggttctc
     1981 tgaattcaaa actgcgcatc ctttcccctt gattctaaat atggaaccag attgagtccc
     2041 tttaggaatt ctcaatttta cctccccgag gattgtatcc acctgaacat cacctccaag
     2101 aactgctctg tgaactggga tgctctcctc ggaatatata tcattccctc tcctctcaaa
     2161 tttctcagat ggttcaacct ctatctccac atacagatct cccacaccat cccttccgac
     2221 atttccacca ttcccaaatt taagaaccat tccatcgtat gcacccgcag gaatctttat
     2281 ctttacattc tcctgcatag actttactcc acttcctcta caagatttac atggattttt
     2341 aatctctttc ccagttccgt gacactttga acatgtggac ataaccgcta tttgtccaag
     2401 aatcgtagat ctaacttttc tctcctgccc acttcctcca caaacagaac acttttctgt
     2461 tttaccatct gcactaccac taccattgca tacctcacac tctacatctc ttgttatttt
     2521 tatctcgtac tctccacctc tcatggcctc atcaaaactc aatctcaccc tgtacctgat
     2581 atctgcccct ctatctactc ttgattgctc attgaaacct gaaaaattaa aaccaaaatc
     2641 gttactccct ccaaaaaaag tattaaatat atctcccatg tcaaacggag caccaccaaa
     2701 atcgtatcca tttgttgagc caggattaaa acctgcagta ccagcatgtc cgtattgatc
     2761 atatgctgac cttttagatt catccgagag cacctcatat gcttcttgaa cttctctaaa
     2821 tttttcttca gcatcaccat ccttatttac atctggatga tatttcttaa ctaattttct
     2881 gtatgccttt tttaattctt cttgtgtagc cttcttgtct acaccaagaa cttcgtaata
     2941 atctcttttt gccataacca aagattctag caaacaatac aattatatgc tagaggtata
     3001 tttatacatt aaaaaaaatc tttaaatctg gtaatatcaa atgctatgac aaacgaatgg
     3061 aatgtcccta caaaaaatga aaagaaaact aaaaatgtcc aactctggat ctctggcata
     3121 cttgccttta ttggtctggt tattgtaagt acgcaattaa ttccgttaac tggctccttc
     3181 ttggacggtt taatagagaa actaagaaca gagtttaaaa ttaagccggt accagactca
     3241 tacaaacagt atatcgaaga acaattcgct tattatgatc caggaaaaag ttacttcgct
     3301 aatcttgcag aacaaattgg agatatagag atagcaaacc aatactctta tgatccagaa
     3361 tctgggactc agaaggaaat caccgttgac acaaagtatg acaaagatat gtatatctct
     3421 atctctagta tcggaattaa agatataaaa atatctccaa atgtagaaag tagcgatgaa
     3481 aaaatatata acaagtattt aaaatctgga gtagcacatt ttaaaggaac tcctctacct
     3541 ggggatggtg gaaattcatt catttacgga catagtgcag tagattcttt ttttagaaat
     3601 cacaaaaatt tacccgaaac gatattctca agactaaaca gtattgatat tggtcaggat
     3661 gtcgtcatta ccaaagacaa agagacattt acatatgtgg taagaaacaa aaaaatagtt
     3721 gctcctgatg atttcagtat tctgcgaaca cagaacaata aagaaacagt aactttgatg
     3781 acctgttggc ccctaggtat aggaacaaaa agattggttg ttgtagcaga gagagaaata
     3841 tagtttaata cttatatata tggaatttaa gaataaaaaa ttaataatta ttattgccat
     3901 cgtcctcttc ctccttttgg ctggaacagg aaccctcctt ctactttcaa aagataatgg
     3961 aaataataat aacactgtaa aaaatgttga gctagtatat tggggactgt gggaaccatc
     4021 ttccgttatg caacctttaa ttgacgaata cgaatccacc caccctggag taaatatcct
     4081 ttattctcag cagacttttt ccaattacga aagtagactt tttacaagac ttcagcaagc
     4141 atctagctct acagaacccg ctccagatat tttcaggata cacaacacat gggtcccaaa
     4201 gtactattca tatctttctc cacttccaga aaatgtaatg actagagatc aatactccca
     4261 aaccttctac cctacagctt taaatgattt tactgctaaa gatggtaaac tctatgctat
     4321 tcctcttgag atagatggct tagtactttt ttacaacaag caattgctta cagatgctgg
     4381 attttctgaa cctccaaagg attgggattc tctcatagat ttagcgcata aattaacaaa
     4441 gaaagattct tcaggaaaga ttacacaggc tggattggca attggtacgt ctagaaatat
     4501 tcttcatgcc tcagaaattt tctcattttt gcttcttcaa gaaggagttg agcttattga
     4561 ttccacaaga actaatgtaa ctttaaacac cacaaaggtt ggaagtatcc tcaattttta
     4621 cactggattt gcaactggta aacaggcagt ttggtcttcc gacttaagat atgacttgga
     4681 gatgttttac tctgggaaat tgggaatgat gatagctcca tcgtggagag catttgatat
     4741 aatcctttcc tctccaacaa ttgaatttgg tatggcaaca cttcctcaac ttggcgccaa
     4801 ccaagaaaaa atctactatt caacttactg ggcagaagca atcaataaat cctctgagaa
     4861 tacagatgtc gcttgggact ttgtgaattt cttagcacag aaggaaaata tgatgacaaa
     4921 attctccaat gaatccaaaa ttagagcatt tggagagcct tactctttgg ttgaattaaa
     4981 cagtagtatg tcaggtaatt catacctaac agccattgct gaaatggcac cgtatatgaa
     5041 atcttggcag atgggagatg aaactattgt taaacagact ttaaatcaat taataacttc
     5101 agtcgttgag gatgggcaag aactgaatgt cgcccttaaa aatgcggaag aagatataaa
     5161 cgatcaactt gcacaaacca ataaataacg tctattatat attaatgcaa agaataaaag
     5221 ccccaagaaa atctgcccaa atatctgtca tatatctaac cttctttctt ctactttcaa
     5281 ttccacttgt tgtatttggt gttttaaacg gtaactttga tatccgaaat agagctttcg
     5341 atgatttgga agtcagcgaa gagaacccat gtataatatc actcccaaat gttaatccct
     5401 ataccttaga agttgggaga ccgataacaa tacaggtaga tgcaaaactt tccaatgcca
     5461 taatttcaga attaaatatt acagattctt ctggaaattc aatatatacc gaaaaatttg
     5521 acaatgctcc acttgaaatt gcaactagct ttaaatttac accaacaaaa tccggtgttg
     5581 tagatctctt aggaattatt aaaaaagacg gtggtggaag tgtaggatgt aagataagta
     5641 gtccgtacga tatcctaggt cttcgagcat tagcgaataa tagtgctcca gtattcatat
     5701 ctcaaccctc aagttcaaaa ccatctcaaa gcattaaaac tggaacagtc tatgaatata
     5761 ccctctctgc agtagatgaa gatgatgaca gagtaaatta ttcctactca ttcacaccag
     5821 aggcaaagtg gctaaagcca acaataattg aagatggatc ttctgggaaa ctaactctca
     5881 aatttaccgg ttcaacaaat gtgcctggta gctacctagc aaatgtcttc atccacgacg
     5941 gttacagtat gcatctagca tcacagtctt gggtaataag tgtcagtcct gcaaaaaatg
     6001 acatccctat agtcaaattc ataagtcctg tatctccaat gaccgtaaca caaggtaaaa
     6061 atattacagt atcctgggaa gcaaatgatc taaacttcat taccaaatac caactttaca
     6121 tcacaaagaa ttacacaaat acagaaaact ggattccaat taatactaat atccctttta
     6181 aaacaagaaa tttcaatttc tctacttcca acctagatac aggtacatac aaagtcatac
     6241 ttaaggcagt agataaccaa tccccacaag tttcaggatt agcaatctct cctgaaattg
     6301 taattacaaa atctggacaa tctaccattc ctacaaacga tattccaatc atagatgcac
     6361 cacaggtcat aaatatgata cctctcagta cagataaaat aaccaataaa cgagtaacca
     6421 ttaaagcctc tctaatctca gcagaaaaag cagatattga cgaaaagaca atactcttta
     6481 aactagatag cgtagataaa acttctgaga taaaaatcaa caagatatct actcaagaat
     6541 atacaatcat ttatcaacca ttagaagatt taaccgaagg gatacacaaa gcagaagcct
     6601 atttcaaaga ttccgctgga cacgaagtaa caaaagaatg gacatttaca atacaaaatt
     6661 ctgtagaaga attaaaagat gaaactataa acatcttcgg tctagatata agtaagagaa
     6721 tccttctaat tgtaggcata ggactagttg tcgttatcct cgcaatagta gctccaatca
     6781 tcatttttaa tgtctggaaa gaagataaaa tgagagatca ggaagaacca cactctaatg
     6841 aaccaataca accattcata cagcaacaag aaccagaata tatcgaacct ccagttcaag
     6901 cagaagagat aaaagagaaa gtggaaaaac aaccagttga agtaccagta aaagaagtga
     6961 aagaaccagt tatctttgct gctcctgaac cagtaatcga agaagaaact actccagctc
     7021 ccacagaaat actgccccca gaaccagact taagcacaga tcttccaact gatgacacca
     7081 taaagcaact tttcgagcag gtacagaaag ctcaagaaga acctccacaa acaaatagct
     7141 aaatagaaat gtacttgata taattacctc tatgaaccat ctttcctaca aagagataag
     7201 aaaaaaatac ataaaatttt tcgaacaaaa caatcacaaa gaaataaaca gtgctcctct
     7261 agttccagag aatgatccct ctgttctttt tgttaatgct ggaatgttcc ctctagtacc
     7321 atttttgcaa ggggaaaaac atccaagtgg aacaagatta gtaaattctc aaagatgtgt
     7381 aagaacaata gacatagatg aagtcggaga tgcatatcac tgtactacat ttgaaatgct
     7441 tgggaattgg tctctaaacg attacttcaa aaaagaagct ttaagcatga cactcaaatt
     7501 ctttgtagaa gaattaggct ttgacataaa caaactctca gttacagtat ttaaaggaga
     7561 taacaaaatc ccaagagata cagaatctat aaaaatctgg gagtcccttt tcaaaaagta
     7621 caatgtagaa ttaaaaggta aaattactga aaatggtaag gaagataatt ggtgggaatt
     7681 accaacaggt ggaccatgtg gaccagattc agaaatcttc ttcaacactg gaaaagaaaa
     7741 tgtggaaatt ggaaataatg tatttatgga atatctaaaa tctgggaatg aatacaaaga
     7801 tttgggaaga cataatgtag atttcggtgg aggtctagat agattggcat gcatctgcca
     7861 agaggtagag agtatatatg agacagatat ctacaaacca atatttgaaa aagtaaaaga
     7921 attaagtaaa aaagatattc ttgaatcaca gagaataatc acagaccata ttaaagccgc
     7981 tacctggata atcatggatg gagttatccc gtctaaaact caacagggat atatcttgcg
     8041 aagactaatt agaagagctg taagacatgg tagaaaatta gaaattgagg gacgctttac
     8101 aagagatgta gcacaggtag caattaatca gttcgcacca atatggaaag agctaaaaaa
     8161 ccaagaaaaa tatatcttag atacaattga ggaagaagaa gtgaaattta gcagaacact
     8221 acagaaggga ttagctgaac tggacagaat ggtttccaga gaagaaatag atgggaaaaa
     8281 agcttttata ctctacgaaa catatggctt accaattgag gttatacagg aaatccttac
     8341 agagaaaaat cgtaaaccag tagatattaa tgaatttaat gaggcagaga aatcacatag
     8401 gaatctttct agaacagcat ccgcaggctt gttcaaagga ggcttggcag atacatcgga
     8461 acaatctaca aaataccaca ctgcaacaca ccttctactt tccgctttaa gaaaagttct
     8521 gggaacccat gtataccagc gaggaagcaa tattacccca gaaagattaa gattagattt
     8581 cccaaacgaa gagaaactta cagatgaaca aataaaagag gttgagcata tagtaaatga
     8641 acaaatcaga ctaaaactac caataacctt tgaagaaatg gataaacagg atgctttaag
     8701 catagtccct tttgctgcat ttgaagaaaa atatggagat atattaaaag tttactacat
     8761 agggccaaaa gataatccat tctctattga aatatgtaac ggtccacatg tcaaaaacac
     8821 ttcgcagtta ggagaattta aaattgtaaa acaagaaagc ataggctccg gaattaaaag
     8881 aattaaagca atattaatat gacagagtat ccagttttag caatagactt tggtaaaaaa
     8941 cattttggaa tagcaatctc agattcaaaa ggcattattg cctctcctct tgaggttata
     9001 tcaattacac aaagaagagg aattgaatat attcttgaga gaatctctaa tatatgtgaa
     9061 gaatataaaa ttaaaactat ccttcttgga atgccccaag tctttacaga agcacactct
     9121 aaaacaccaa aagcaattat tagatttgag aaaaaattaa gagaagtatt agatttacca
     9181 atcatactct acgatgagag cttttctaca acttcagcac aaaatatgct aacatcatca
     9241 ggatcaaata ctaaaagctc tagaaaaaaa atagataaag tgtcagctgc ggtattttta
     9301 caggaatttt taaattcttc taaaattaat aatgataaaa agcacaatta gaatcttttt
     9361 gtttgcgtta gttactgtaa ttatagctac tatcgtaatc aagctagact atgacaaagc
     9421 tttgagaact cccaatagtg atagccaaga gaaagtaata gtccagatag atcaaggagc
     9481 aacggtagag caaatcctca caaaacttgt agatcaaggt gtcatacagg agaaatggct
     9541 tacatatgta aaactgtacc tcaaacagga aaatttagca gcaaagcttc aggctgggac
     9601 atatgaatta ccaaagaatt tgagtattaa ggaagtaatt gactctctcc agaatggaaa
     9661 gaatccagat atatgggtca ctataccaga aggcttaaga aaagatgaaa ttgcagatat
     9721 actaaaccga gaactttcta aagcagatac aacaaatttc tctaaggagg aattcttgag
     9781 attaacaaca gacagagcat acatccaaac acttggttta accgtaaata tctcagattt
     9841 agaaggttat ctttttccag atagatatgc attcccaata actacaaaaa cttcagacac
     9901 gatagataag atggtgaaga atttcattac aaaggttggg acagcagata catatgatca
     9961 aattatctta gctagtattg ttgaaagaga aggattctct gaccaagaca gaccaattat
    10021 tgctggcata tttatcaaaa ggctagaaga aggatggctg ctccaagcat gtgcaacagt
    10081 tctttatcca ttaaaagatt ggaaacacga gattacagat gcagacaaaa aatcagatag
    10141 cccatacaac acatacaaaa aacctggact tcctcctact ccaatttgta atcctgggct
    10201 agaatcaata aatgcagtaa gaaattacaa aaagacagac tactattact acattcatga
    10261 caacaacgct atagtgcatt atgcaaagac tttggaagaa cacaatgcta atattaacca
    10321 atatttaaga taaaatacct gccccaatga ccatagacat acagctagat gagaagaaac
    10381 ctgcaaaaat tgtcactcca gaattaatca caaaaagtga aaaaccaata aaaatagaat
    10441 ctcctagaaa aaagaaaaag tggtccatat ttgtccttgt gggaattgtt cttgtcatat
    10501 taggtggctt ggtatttctg gtttacaaag gaatagcttt ttcaaagaat attggtttca
    10561 atttcaatgc caacgatata accgcaccag agaaagagcc tgaattagcc aaggattctt
    10621 ctggaaaata tacaaatatt ctaatcgtag gagttgatac tagagataaa gaagcattat
    10681 taaacacaga cactttaata cttgcatcat acaattatga aactaaagat atcgttatga
    10741 tttctactcc aagagacttc atggctgaaa ttaataattc caactggttt aacaaaataa
    10801 attctgtata tgcaagtgca gaaaataaaa caaaaggttc tggaatgatg gctttaagaa
    10861 aaactataga agaagttaca ggtacagaaa ttcagtacca tgcaatggta gatttcaaag
    10921 cttttgaaaa aataatagat gctgtaggag gtgtagatat taatgtggaa aattcattca
    10981 cagactataa atatcctgat ggatactcct acaaaacgat ttcttttaaa gctggcccac
    11041 aaactatgga tggaaaaact gctttggagt atgcaagatc cagacactca cagcaaaaca
    11101 atgaaggtgg tgattacgca agagcaaaaa gacagcagaa agtcatagta gctcttcaag
    11161 agaagattct taatacaagt acactaacaa gtcctaaagc cattatggag atatttgcat
    11221 ctgtagcaaa caacataaaa gtctccgaat tcacaataga tgacatacaa gctggattaa
    11281 acctagccaa ggatttccaa gataacaatg gaaaatcata ctcatttgtt ttggatcctt
    11341 cagctgggaa tagtcaaata atttcgaatc agaatgtttt cggtccagat ggtaaaacag
    11401 ttacagatta tcgtatagga cctaagcttg gattaggaaa ttatactgac ataaacaaat
    11461 atgttaaaca aatacttctg cacccacaac tttacacaga gaacgcctct gtctatgttt
    11521 acaatgtagg tttaggagct caggaagcaa gtaagaaagt tgcagaactt aaaaaagcat
    11581 acccctacct taaaattatc tactcaggta caaaatattc aaataaagaa ggctctgcaa
    11641 tatactcaaa tactccagac acatacctaa ggacagttac agagtattcc aagttcttag
    11701 gaatcacaca aacaacaaaa cctgattaca ttactaccaa tctaaacggt ggagatgtta
    11761 caatcctact aggaaaacct gtacaattaa gtagtgaata acaaagtgaa ttacaaactg
    11821 tatcatggaa attctgtata cctctctttg agagcagtta agaaagacat cgctacaaat
    11881 actgagaaga aaaacttagt aaccattgag gcagatactg agaaagcaca aaagatactt
    11941 gacagtatcc aaaaccaagg attgtttgtt tcaaaaagag ttttcctgat taaacgtctt
    12001 tacaaaaata aagagaaaga ctccctcata gaaaaacttc tctctctttt aaacgaccaa
    12061 tttccagatg atatatattt ctgggaagac caaaagatta gatctactac taaatatcta
    12121 aaattcttta aggcaaacaa ctctttagaa gaatatagca gtttaaacaa acgaaatttt
    12181 ctctcttggt taaaccaaga gcttaaagag aacaatctta aggcagatga aacaacaaag
    12241 agattgcttg cccaaagaac caattacgat ccagagagat gtgcaaatga aattaagaaa
    12301 cacaaattaa acgatacaga agaaataaaa gaaaaagatt tagatatttt aaccacagat
    12361 actctagagg aagatatttg gaaattggta gatgccataa attcagagaa aaaagaaaga
    12421 agtgtagaaa tccttgaaaa gctctattca caaaatacag accccaattt catactctca
    12481 atgctcgcaa gaaacctcag attaataaca atggcaaagc atcttaaaaa acaaaagaaa
    12541 agttttgcag aaatatgctc tattatgaaa gtagctccat tctctcttta tccaatactt
    12601 ggagttattg atagatatga catggagaag ataaagcttc tgtatactaa actctctaac
    12661 ctagattacc aaataaagac tggaaaaata gatggtatgt taggacttac attaatatgt
    12721 ccttacttgt aattgacacc cttcccatct taacttaaac tttaaaaagg catattcttt
    12781 aatacaccct ctgccctaat ggaagaagag ataaaagtaa caacaaaaaa gaaaaccagc
    12841 aaaacagtta aaatattgtt aatcataact ggaattcttg gacttgcact cgttgcagtg
    12901 gcaatctact tctactccat cgcagatact tccaaagatg acgaaattaa acaaactaca
    12961 tgtggatgtt actacataga tccaagcgtt gtatcagaat gtggagatcc aaaaagagga
    13021 ttcatttttg aaactgttac cgttccaagt actcaaacct gcaaggctgc ttgttctact
    13081 agtaagctct ctgtaaacat actaaatagc actacagaac aggacctcta ccaaatatgt
    13141 cagctaccat caatactgga ctccagatgt actgaaatgg taatcaagga ctctaccgga
    13201 aagattgtaa ctggaaaggt ttctgcaaat gatgtacttt cagttgaggc gaaatttaat
    13261 caagaatata tagactacca attcaccata aataatgaaa caaaattacc agacaccata
    13321 tctacagaca aactctccat cacaaaatcc attgctgatc tttctggtat tactacccta
    13381 aatatcgttg caacaggcaa aacatccact ggagagcaga ttaactcccc tctttgtaga
    13441 agattaatcg aagtagcaca agacggagta aagaatgttt cagggattca aattcaaaca
    13501 agaacagtag atacaaagct taaaattgca aagataaaga taaatgcgag caatataaca
    13561 gatgaggcaa aagttacact gaaattctcc tttagtaata acttcccaac cctctctatg
    13621 aaagaaggat ttactgcgaa taaaacaaag ggagagattg aaatcgtaga gcaagacctc
    13681 tacaattcat caaactttac cgaagtgaaa agtttttctc agttagataa tcacaaaggg
    13741 gaattaacaa taaccgcaac tgtattagaa aacggtaaat ctctaggatc cgtttcaaaa
    13801 gcaattacat tccctgaaac ttcatccaat gtagaaaatc cagatgcaga tacaacagaa
    13861 gaatctaact ttaccattgc caaaaccgca agcgaacagt gtgtagaaag agtagctcca
    13921 aacaactttg tcacatttac ccttacctct actaacagag gaacaatatc tcaagggata
    13981 acctctgtta aggataaact ccctctcggg tttacatatg tagctaactc atcaaagata
    14041 aataatgttg tggttaccga ctcaaattat gtaaccgtca gcacaatagg aagtacgcaa
    14101 gagatagtat ggactaaaac agaaccatgg atagtaaaca gtaatcaagc attgacaata
    14161 acctttaatg caatagctgg tggaaatgca ttaacaggaa aaaatacaaa cgaagtaatt
    14221 ctaaccccac agcaaattcc cgcagaccca tcatctctga gagctactgc cacacttcta
    14281 gtacaacagg attgcgataa tcctgaggaa gaaattccag aagttccagt taatccagat
    14341 gaagatattc cacaaacagg tatctttgac tccgtaatct cgaaagtcat actcggaatt
    14401 ctaacaatta catttggatg gtacatatac agcaaacctt ctggcagagt actagcagaa
    14461 aagctcatag agacacctgc cttcaaggga gcagaaatga ctacttggaa aatctttaaa
    14521 ccgaagaaat atttcgaaga aaaaataata aataaaattt ctaaagtgaa tcagaagaaa
    14581 agataacagg tgaaccgttt gaaccataac tcttttctcc agaatttatc aaaaaatagt
    14641 agtgctctga actcaatata ttctctaaca ccacctcatg agttttagat ttcgctttat
    14701 ttgttaaatc cacagctact aaatctaatg aattactact attcccatac aaaacaaatc
    14761 ctacacactc tgcctttgta acccaactaa tcttgacaga atattccttc tcccctttct
    14821 ctacaaacac attgtaaggc acacaatcct tcctaatata tcctactaaa tcaatactgt
    14881 ttgcctcttt tacgaaaagc ataacagatg acaaaatagt tactaaaaaa gagaccgctc
    14941 ctaatatcat aaatatcttc ttactcatct tttgcttcta ctaaaatata actctcttgc
    15001 acagaagaat tatctccatc agagacccta attacaagcc tgtatgttcc aacacttcca
    15061 aatggtgctg taccagatat tttcaattcc gacactccca accaacttgg tccctcaagt
    15121 aaatttatcg ctatatcttc catctccgta tcattatctg tcaccttaac catatatata
    15181 tactcctctc cctcatacac agagatagga gccaagcttg taatccttgg aacggaatag
    15241 agatcttgat ttcccaaaac ttcatcattt gtatctttat ctggagaata attcaaaaaa
    15301 gaaaaaactg caccaccgga aattagtaaa aatactaaaa aactaagaaa aatttttact
    15361 tttctacttc tctccattta aaaagtttaa caacaaaaca aattgtttac aaacaaaaga
    15421 ataatatata taataccttg attcacaagt ttaaattaca tgtattaaca atgccaaatt
    15481 taaagacatc tattaaagat ttaagacaaa ctaagaaaag agaaaaagtc aacgacagat
    15541 ttaagaatag aataaaaaaa gccgtaaaaa aaacagattc tctgattaaa gatggaaaag
    15601 atacagaggc aaaaataaca ctaaaaaatg tttacaaagt tcttgacaaa gctgctaaga
    15661 aaaatgttat aaagaataag aaagcagacc gaattaagtc tcgtctttct aaaaaattaa
    15721 acaaactgac cccaaataat gtcaaaactg ctaaaaaggg cgcttaaaat atccgtgata
    15781 ccagcaatat tgatgattgc tggtaaattt ctgggtatat ttgtaacaag tgttctttac
    15841 gaccttcagt ttaatataga taacgatata tctggaatct tttcagtcca gatatatttt
    15901 actgatccag agacaaccct ttttgtcaat tccatgtcta accttacaat gattctttct
    15961 ctgttactcc caatactcta cttcataatt aagacatatc tttatactga agcacacaat
    16021 aatccaaaaa caataataaa aatgacaaaa ctaaatctcc taaattggat tacgaaaaaa
    16081 gatacatctt ttctcaagac attcatctgg tgtgcattcc tttggctcgc aactgcaatt
    16141 acaataactc atagcataca aggaaataca tactcatgga ttgcagtaac ttctggagtt
    16201 attgcccttt tggtaactct tttcacaatc aagacatttg aattagaaac tgataagata
    16261 tacccaatag agaaaaaata tttttaatat gcccagcaaa ctaagaagcc cacaattcaa
    16321 aaaagtagta ggacagatac tagaaccttt tgccctaggc cttttagccc tcttgttcat
    16381 aatccccact cttacagttt ttaacctttc tccaataaca aaaacttttg aaaaactgaa
    16441 tgtccttgga gtatcctcaa acgaagatgt gtcatttaca ctcgtagagg gtaagcatga
    16501 aattattaga agtgaatatc tagttaaaaa tgaagaggga gattacacat actctactac
    16561 attaaacaaa cgagcatcag acagttactc taagccaata attgagctta aaaacaacac
    16621 ccaagaacaa caaaccattg ttttgtatgg acaaacaaaa gcaccaacaa gatcaaatat
    16681 atctgtaatc ataaatgacc atccatataa gcttcaggat gaatcaaaca atacatatcc
    16741 acaagagata actatagatc caggaaaatc tacaattatg tttttggcaa ttgagaatct
    16801 ctcaggagta caattttctg aagaatttga aatgcaccta acagttaagt cagatatcta
    16861 aacaatacaa aaatcctctg tattgggata taatccttct atgctagaaa aaattagaaa
    16921 tttctcaatc attgcccata tagatcatgg caaatctacc ctcgcggaca gaatactcga
    16981 agctacacat acagatcttt cccacgagaa aagaaaatct agagtcttag atagattaga
    17041 cctggaacaa gagaagggaa ttacaataaa acttcaagca tgcaatatgg tatggaaagg
    17101 ataccaactc aatctcatag atacaccagg acatgtagat ttctcctacg aagtatctcg
    17161 ttctcttgcc gcatgcgaag cagcaatact tcttgtggat gctagccaag gaatacaagc
    17221 acaaacaata tctaatacaa agaaagcttt agatttagga ttaaaattaa tccctgtttt
    17281 aaacaagata gatttagatg gaatagatat agaagaaaga gagaaagaaa ttacagaatt
    17341 acttggcttt tctaaagatg aaatcattaa agtctctggc aaaacaggat taaatgtaga
    17401 ccaacttctt gaccgcatag tagaacaagt cccccctcca gttggagacc ctaacaaacc
    17461 attaaaagct ctaatctttg actccttcta cgacgaacat agaggtgtag tcgtagctgt
    17521 aagaatcttt gatgggagaa taaaacatat acatggaaaa ccaattgaat taaatatcct
    17581 tcaaagaaaa caaaccttca gaccaacgga aattggaatg ttcacaccag atttagaaga
    17641 aactggtgaa ttaaatacag gtgaagttgg atacatagct acaggactta aagacattag
    17701 ctactttaca gtaggagata caatttctga cagagaagat acacaaccac tggcaggata
    17761 caaaacacct aaaccaaata tatttgcaac cttcttccca acagattcag atgagtacga
    17821 caaccttaaa acaggattaa acaaattagc attaaatgat gcatctttaa catttatgga
    17881 tcaaagatca aaccttcttg gctcaggctt tagatgtggc ttcctaggcc tactacatat
    17941 ggaaatcgtg caagaaagac tagaacgaga gtacgacgcc agcctcataa ttacagcccc
    18001 cacagtagag tacaaagtca ccacaacagc aggagaagag atatctgtac aaacaccaca
    18061 agaacttcca gacccttcag gcataaagga aattagagaa ccatgggtaa gaacagaaat
    18121 actcactcca tccaaatata tgggagacct aatgaaacta tgtcaatcca gaagaggaga
    18181 atatgtaaat acaaaatacc ttacaaccaa tacaagtgaa cttaaaatga gagaccaata
    18241 catagcctta gagtatgaca ttccacttgc ctccctcatc tcaaatttct ttgatcagat
    18301 gaaaaactta acaagtggat atgcctcaat ggagtatgaa ttcatagatt tcttcccagc
    18361 ggacattgta aaagtttcaa ttttcgtaaa tcatgaagaa atccctgcat tgagtttctt
    18421 agaggtaaga gatgaagccc gttccaaagc tacaaaacta ctagcagaaa tgaaaagagt
    18481 aattccaaga caacagtttc caattcccct acaagcagct attaattcca aaatcatagc
    18541 aagagaagat gttagtgcat ttagaaaaga tgtaactgca aaactctatg gaggagatta
    18601 ctctagaaaa aagaaattac ttgaaaaaca gaaaaaagga aaagagagat taaagaaaat
    18661 tggaaaagta aatattccac aagaagcttt cttagccata ttgaaaacat aatgattaca
    18721 aaaatcaaat taacaaatta cagaaaattc aaagatcttg aattggatat tacaaacaat
    18781 atcgtagtat tacatggtga caatgccaaa gggaaaagca ctgtacttga agccatacac
    18841 atcattacaa acggcacttc cccatggaca tcctcggatg attacatcaa cactgaacag
    18901 gatgaaaatg aaaaattctc tagagtagaa ataatagagg acgagaaaac ctactcattt
    18961 atgaaagata attccaagag agtttttaaa atagataacc acactactac gcccaagaag
    19021 ttcttcgaac aacacgcttc cataattttt aaccctgaac aaattgaaat ccttatgatt
    19081 tctccatcca aaagaagaga tttccttgat gaggttatct ctaaaatcaa ctacgagtac
    19141 agcgagacat tagctcagtt taagaaagtt cttcgacaaa gaaatgcata tcttaaaaaa
    19201 ttatctaaga aattctacga gcaagggata attgcaagaa atgatactca actaaacttt
    19261 tggacaaatg aatttgtaaa attatctaaa gatattcaaa aaaacagaga agaaatttct
    19321 cgagaacttt cctttgggaa attccatatc gactatataa aatcaaacaa aagagatagt
    19381 ttagaaaatg aaatcgagaa ttcaaaaaaa agagatattg caacaggata tacaaatatt
    19441 ggaccacata gagatgattg ggatattatt aatgggaaaa atataaaaag atttggctct
    19501 cgtggagaaa aaagattagc aattgggcag ttacttttta aagttcagga tttaattaat
    19561 tcagagcttg gttactaccc aatacttctt ttagatgata tctcttctga attagataac
    19621 aaaaatacaa aaataatctt tgacaaaaag aatatagtta aacaacaaac attcattaca
    19681 ataataaaat atgatgaatt accaaaaaat atattaaaac aggcacagtt aattgatttg
    19741 aactctattt agcactctcg attttttcta ttttatcaat actcccttac ggtagctctg
    19801 tttttagagg aatcgaattc gaccccttta aaacttttat ttcatttaat caaagtttca
    19861 taatcaccat atacattaca gtatatgaaa aaacaaatca tacaagtaaa aggtgaagat
    19921 atagaattga ttgtaaaaaa caatcaagac tatatatccc tcaccagcat agctaagtac
    19981 aaaaatactc ttcatcctga tgatattatt aaaaactggt tacgaaacaa gaacacctta
    20041 gaattccttg gtctatggga gaagattaat aatgcgaatt ttaaacccgt cgaattcgac
    20101 gggtttaaat cccaagcagg tacaaataca tttgtgataa caccaaagca atggatcagg
    20161 aatgtgtctt ctatcgggat aatatcgaaa cctggtaaat atggcggagg tacatatgca
    20221 cacaaagata ttgcctttga atttgcttca tggatatcac ctgagtttaa gctatacctt
    20281 ataaaagaat ttcaaagatt gaaagaagat gaatccagaa gaaattcttt ggattggaat
    20341 ttgacacgaa cattagcaaa agtaaattac aaaatacata ctagatcaat aaaggaaaat
    20401 atcgtgccac aattagttac caagaaacaa aaaacttttg tatatgcaaa cgaagcagat
    20461 gttttgaacg tcgctctttt tggacttact gcaaaagagt ggagagagag taattcagag
    20521 aaggaaggta atattcgaga ttatgcagat attagacaat tgatatgcct atccaactta
    20581 gaaagtctta atgctgaatt aattcgtgat ggtttaagcc aaaaagaaag attacttaag
    20641 ctcaatatca ctgcaatatt ccaaatgaga gcattaacag gtatagaaga tcccttttgt
    20701 ccactctgtg ctttacttta aataatcttc tagcttttcc tttttaaaat cttcttcaaa
    20761 aaactctatt cttcctttct tgtaatttct ttctatcctt tttaaatctg gtttatgatt
    20821 tgaaatatac ataaaagata tctggagtag atagaaaaca gtatttagaa atttaataaa
    20881 aaaattagca ttctttcctc tagaaatttt ggtatacaac aaatccttct ttacaaaata
    20941 tgactctctt aaccaaggat tgtttgaata aataaaatta agatacttct cattgtatat
    21001 aggaacaaga aacattaatt catggaaatt aaatatgtca ttgtcgaatt tcaaacctct
    21061 ctcctcacat atcatgttta aacagagttt gtttttaata actttatgcc tttttgctct
    21121 aattttgtta tggaaaagat tagctataga tatcaacccc ctgcataccc atgctgttcc
    21181 atcttttaca acaacaaata catcaatgtc atcttcctct ttcgcaaatc ctgctccaac
    21241 tgaaccagat actcctatga atttaaccca attaaaaatt tttaatactt taactatatt
    21301 ttctgcatat tctaatttcc cttttgctat ctcgttctca ttttgttcat acttttcctt
    21361 agattctctt aggaaactct ttccccaaat ccttttaata tcatcttttt ttaatgatgc
    21421 acccatca
//
LOCUS       DAOQWP010000014        19170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_3237_length_19170_cov_7.478002, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000014 DAOQWP010000000
VERSION     DAOQWP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 19170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19170
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3237_length_19170_cov_7.478002"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKI16_02400"
     CDS             <1..537
                     /locus_tag="PKI16_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53301.1"
                     /translation="TIQRESMLLERTPGEDWLYGLGIDFTKTTTTGVYKVFKWCSPFP
                     DYGDTKTKSKLPAYDPAVNLGIGRNGYLPTDRTIGTSSSCNGEGLATFPGYDTSIDFP
                     ESEITIDNGIGYVVFESISGRAFFYDANGELVNYQADGKQAENVFNFVLTIDPLGVGK
                     TRQFVVNNLSGKISSLVL"
     gene            534..1055
                     /locus_tag="PKI16_02405"
     CDS             534..1055
                     /locus_tag="PKI16_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53302.1"
                     /translation="MTKHIEKKYEALGFVEALIAIMVVGASSVILMQIAVNTMQGVIQ
                     NEKIDTMTQIALEGAETVKEIANQEKFTGEDLFPNLAGCYVPLSEAGVFTFKKGLDGN
                     FLYLEADANRGEYVSNAAINEEFFRIYCFESYSVGDRYVSGNIIVGELNSEGTITKGN
                     NVKDYYYFTVVNL"
     gene            1052..1762
                     /locus_tag="PKI16_02410"
     CDS             1052..1762
                     /locus_tag="PKI16_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR53303.1"
                     /translation="MINLKGNKYEGFSLVEMLITIVILGMVMLIASVTLTTLIKISTV
                     SSNKTRVRNETEFVLELVRRTVRNSNPADVMIYNSLDSRMYDSENGVVRNNPEIADIS
                     SVYAVPLGENVTGNEIHFRPYGYTNWICIGFFRDMDPESEMGYVIKTSTQDLWGNAQK
                     CFDGTTEDYRMYTIVLNSENVDINSFNIAYTESTGGNYIIRFDLSAQPISWYLASSAP
                     VNREVFRQAVVTTEGINW"
     gene            1811..2188
                     /locus_tag="PKI16_02415"
     CDS             1811..2188
                     /locus_tag="PKI16_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNR53304.1"
                     /translation="MGKTILIIEDEKDLLDSYKELVESVGFNSMVATDGYQGLDILAS
                     QLGNIDLVLLDLMMPGVDGLEVLKAMKTNKEKYGDIPVIILTNMTSDTVIKEAFDLGA
                     SSYLVKIDLDYEGLVKEIEKYLG"
     gene            2251..3282
                     /gene="pilM"
                     /locus_tag="PKI16_02420"
     CDS             2251..3282
                     /gene="pilM"
                     /locus_tag="PKI16_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNR53305.1"
                     /translation="MSKLPDHVGIDFGTHSVKAVELKDIFSETPELVNLGSQITPKGV
                     INSEDKLDQKKLADVLKKLYDSSNIKNKNAVMALPEFSVFTRFLEFPGVKKEELKDAV
                     YFEAKQYIPMPLEEVQLSYVPIGFNQEKNAPRVLLVAAPLKIIDIYINIATLAGLELV
                     AIETESVAMGRAMYKSTKKKNLVMLDFGANSTDMSILSDGYLVFSQSIAIGSDSLTQA
                     IINKFSFEYPRAEEFKRNYGVVENVLEGKIYNVISPIVESIMVEVRRGVEFYKNKTLS
                     ASPTDFILNGDGALLPGLPEYVSKSLGVTATIAMPWNSIKIDNKLKDIIAKSGPSYSV
                     AIGLALKDE"
     gene            3275..3940
                     /locus_tag="PKI16_02425"
     CDS             3275..3940
                     /locus_tag="PKI16_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53306.1"
                     /translation="MNKVPIQNKKPEDALLINKKKETPIKKVTPPTPPTKESTGINLI
                     PNLSKEEVIKETKKKTLNMGAVISLMALVVVSILVVGFNIISKIQLNSEKEKLYNYEQ
                     RLSSVSYKITGNNEIRERISLYKDIQKNAFSPRTVIEYINNIASKSGSIKLSSFDFGS
                     QLSFDIDGEAYSYEEVSKFWYLLSNDSKVESIVLKSVGKTETGVRFTFQGKLVFTDFV
                     SST"
     gene            3940..4656
                     /locus_tag="PKI16_02430"
     CDS             3940..4656
                     /locus_tag="PKI16_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53307.1"
                     /translation="MANLIKKNVKEEKIKKEIEAVSEKIQKTRLTFTDILVPIVAVIV
                     LVVLGIFVFIPMIKEALAVRNEYVDVKAKEKQLEQLEIALNNLDETTLQADLVNAKNV
                     IPKTLKVSSFIYYIDNLAFEKNLSSEKISAGDIKIGGGEKKEGKYILGVSGPLAYSGS
                     LENILSFLESLYSASPYIISVENISLSESSKDWTVELNVTGYYVPENIDEFNLYSKFE
                     PYTTDDNVVKIFESKAKELD"
     gene            complement(4653..4979)
                     /locus_tag="PKI16_02435"
     CDS             complement(4653..4979)
                     /locus_tag="PKI16_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNR53308.1"
                     /translation="MKELLEHIVKSIVNHPDSVSIEEKESVDFPGLTILTIDVAEEDK
                     GVVIGRKGRTINAIRDLITINAIRTDRRVKVLIKDDKAGNREKPQKEAPQEGNSTEGD
                     MLYDEI"
     gene            complement(5021..5320)
                     /gene="rpsP"
                     /locus_tag="PKI16_02440"
     CDS             complement(5021..5320)
                     /gene="rpsP"
                     /locus_tag="PKI16_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNR53309.1"
                     /translation="MLKIRLKRTGRRGDPHYRIVVIESSKGRDGRPIEELGVYSPRSN
                     PKVFEIDQEKAAVWLKNGAQPTDTVAQYFVKLGLLKSLKRGSHKPNTEKKTKEQE"
     gene            complement(5349..6068)
                     /gene="rnc"
                     /locus_tag="PKI16_02445"
     CDS             complement(5349..6068)
                     /gene="rnc"
                     /locus_tag="PKI16_02445"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNR53310.1"
                     /translation="MKKQMEKFNDIQKNIGITFKNPQLLEIALTHRSYLNEHQGAKIE
                     NNERLEYLGDAVLELIISDYIFRKYPGKAEGELTSIRSAVVRTESLAEESRKLGVGEN
                     LQMSKGEKDSGGENKDYLLANAYEAVLGAIYLDSGMEECVNFVNRTLVPKIDRIVENN
                     LFIDPKTQAQEIIQAKYKTTPTYVIVKEEGPDHDKKFTVALLINNKEKAQGQGTSKQK
                     AEEGAALAAIEKIGKESESLN"
     gene            complement(6043..6477)
                     /gene="nusB"
                     /locus_tag="PKI16_02450"
     CDS             complement(6043..6477)
                     /gene="nusB"
                     /locus_tag="PKI16_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNR53311.1"
                     /translation="MKRATDPRHLARVLALQELFSKDFNKDSVNTDTIPLTELIELDE
                     ITEYDTKLYEKIKEGVIDNNTEIDSIITKYAPQWPLNQIKLVDLQILRIAIYEGFIGK
                     ITPPKVALDEAIEIAKEFGGETSSKFVNGVLGAIYEETNGKV"
     gene            complement(6478..6651)
                     /gene="rpmF"
                     /locus_tag="PKI16_02455"
     CDS             complement(6478..6651)
                     /gene="rpmF"
                     /locus_tag="PKI16_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNR53312.1"
                     /translation="MGALPKRRISKGRKNRRRAQDGLKVTSLSKCPKCGNMKRAHFEC
                     PSCGFYKEIKETK"
     gene            complement(6714..9437)
                     /locus_tag="PKI16_02460"
     CDS             complement(6714..9437)
                     /locus_tag="PKI16_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNR53313.1"
                     /translation="MALDFDMQEKTICGYHKRNTNKFGILENKFPIFQDENDSIFLLT
                     RDHFFSISTPQITSVIKNYLRYKKFGDIKRIVANILVLPGVFIALASILKYADLLDQF
                     ESITEILESKLVNVLFGISIFNVIILWHDFYEEKSHPIQLPKAKKIQQKEIDEIKATG
                     FKFGRYGHLDTVSFASEQTLELLCNFTKDNQFQTYDLFKYLLKEDYDIQQIVRRSGIE
                     MSVNEMEEKFKIGEDVIPDYPITALRSILTYAIEEAILTESRQLEPQHIFLAILRLYP
                     QTLKYIQAKGISIDILREISLYNNSLIAKAQRTKHLDPNIPYFNKGGIARRWIYGYTF
                     ILGHFSKDLTEEVANNRDIYGIGHDEEIENLVSVLGKLSNKNALLIGEAGVGKSSLIL
                     GVAQRINTGNVPIQLKDKRIVQLDINGLIAHSLKMGNIEELIMKAMQELENSGNAILY
                     IDEMQELIPAKAEESNHSLAGILLPYILNSKFPIIGTVNYSDYKKYFYSNESLRQSFT
                     NIEVKEVSTTDTLKILETKIATLEKNFNCYITFPALVASVELAYRYIKERKLPSSAVQ
                     TIEATCSWAQSNNIQKITAEHVSKAISIQKNINITDIDQEESNKLRKLEENIRKKIIG
                     QDEAVIAITESLRRARTDIRNPEKPIGVFLFMGPTGVGKTYLAKVVGEEFFGNKSDII
                     RVDMSEYQDIDSVDKFLGTTETGNVFGQTNITLVDRVKSNPYTVVLFDEIEKANPKIL
                     NLFLQIFDEGRLTSAAGETVDFTNTIIVCTSNIGSGILLQALEQDEALWGEAKDRAMI
                     ELRQSLSPELLNRFDRVIVFSPLDINQLRNVAELLLTELKERMIDKGINIKWTDQIPM
                     LIANKANEPGMGARPLKRYIQDKIEGQIAKEIIEGNLKSGSEIDIKENWII"
     gene            complement(9440..9841)
                     /locus_tag="PKI16_02465"
     CDS             complement(9440..9841)
                     /locus_tag="PKI16_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53314.1"
                     /translation="MEVKNTVSDSSGQQLLTETKTDSYSAIFLYFIQDFLRWWYVRMP
                     IWHLRKLARISVVVNDNLSLSLLVQNFFLPWHRDYSMMGYVFGLLIKILYIPIALIIY
                     LTSILVYTIIILIWLLLPIGTLTFIFTSILR"
     gene            9926..10855
                     /locus_tag="PKI16_02470"
     CDS             9926..10855
                     /locus_tag="PKI16_02470"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNR53315.1"
                     /translation="MEGKLLKILAPMEDVTDTVFRQILCDIGKPDIFFTEFMNVNGYC
                     SEGKDVVAKRIYFEGIERPIIVQLWGNSPGNFASATKEIAETVKPDGIDINMGCSVRD
                     VLKTGGGSALIQDRNLSGEIIDAVKENARNIPVSVKTRIGYDSVDYSWIGFLLSKKLA
                     MLTVHGRLAKEGYSTPSRWGIFKKIKEIRDEISPNTLLIGNGDIKNEKEGNDLVIKYG
                     LDGYMVGRGVLTNPWLFSGREDIPKKERVKTLKKHLKIFEKTYGENQNFNTQKKYIKA
                     YINNFDDAGKIREELMNTSTLGEIKVKIARYGN"
     gene            10845..11429
                     /locus_tag="PKI16_02475"
     CDS             10845..11429
                     /locus_tag="PKI16_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53316.1"
                     /translation="MEISGQERECTYSEVMVESFAEAVDYLRKETDENESKFCLVDVD
                     GTLFTDNVLKLPVLCHFVPLTISEDIKNSFYSLMSDVFVDNNIAVITNRNNSERILWN
                     SNKVLQHVKDVCEAPLFTSLNRQIPGLNKRNCDNLLSRVMEYVQSKDYLKLYSIEDHS
                     LISPFRNHFLEYLAKRIKYGSGTDVEVVNLVIKR"
     gene            11487..12287
                     /locus_tag="PKI16_02480"
     CDS             11487..12287
                     /locus_tag="PKI16_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53317.1"
                     /translation="MSDFDPTQIPSQSSDGDIPSYEQGPLPGQFTDLKDSTGLSVELQ
                     EVFDKGLEYLKDNPVLSADVRRMEERVVEVVEYPGNVNLFGTTRKTLDKGDLKRVHGL
                     ESYDFKRNGPDSETLKISVRSLKNTAAATEYVKRIEEDYSIYSERADTKVNYAMQGVE
                     ERRNLDVSFFDQSRIYRDRARQEAYKTIEDITFFFEDSEERRICAMVMKKTFGGVFVS
                     LIDFRNPDRAKMFATETPVLLENYNEKKFDSLKQKLSRYVNGMKRDMD"
     gene            12294..12506
                     /locus_tag="PKI16_02485"
     CDS             12294..12506
                     /locus_tag="PKI16_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNR53318.1"
                     /translation="MLHIIVKTNSKDSKVVFENDIYYVYTKSPAKEGKANDEVISLLS
                     EYLDIPKGNINIKRGKKSRSKWVEIV"
     gene            complement(12532..13140)
                     /locus_tag="PKI16_02490"
     CDS             complement(12532..13140)
                     /locus_tag="PKI16_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53319.1"
                     /translation="MNNQDNFEGPNETDKIIPISELNGAVKLQRTIIVGSKPKSYEYY
                     YQTESGPCAALTFMNLWSLVHGKLPNLKEIDQGLINNGTIRKVWTDNFSLIYGNNPNN
                     EEFQLTLGLQNPESRKEKAQSLMDYLDTIRDGSMICGTQQHCNAIHKWDENQYVLVEP
                     NDTQGLKVMTRDESTDYIENRFTNDHNYQDYFYFIGPDINTE"
     gene            13248..14183
                     /locus_tag="PKI16_02495"
     CDS             13248..14183
                     /locus_tag="PKI16_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53320.1"
                     /translation="MTEPTNTTREEESLKDIRLEPGIYDLTPEIRDYITNGIPLSEET
                     LNVLKTFDPEYDFGRDIEERGKDVVLADLDKASKDFNFEEAKQAVQEGLKMAQIINPD
                     IPIKPFPVIFLFIPHYGDAKALYGQGCGINIGALKNKRYGEESPRQKIVSFTAHEATH
                     TFLKQLGKHPEPGNRTWERVALDFIWEEGLATYVEPTHFLPHDTVEADGAFWVDIIKR
                     WYSRENDEDARKIYEDIKERPSFQVWYNYMYYNQPIPKDLNLSEESFETLLKKRNGIG
                     YHVGSYLWKKQIEKGKSLKDLVMQGSHGIDEWMNE"
     gene            complement(14195..14584)
                     /locus_tag="PKI16_02500"
     CDS             complement(14195..14584)
                     /locus_tag="PKI16_02500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNR53321.1"
                     /translation="MRTLVTMHALIVHKDKYLVLQRADNRFNPRYWNCVTGHIKEHES
                     AEETALREVKEETNLDGEIVKTAEPLIHFTKDQRWVILAYLINVNDISNLKIDTNESQ
                     AYKWIEIDDDMIKEYKGLEETIKILNP"
     gene            complement(14602..15192)
                     /locus_tag="PKI16_02505"
     CDS             complement(14602..15192)
                     /locus_tag="PKI16_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53322.1"
                     /translation="MIAHIVAIPTDKDLEYLNSLRLYIYENNFRFSNKSSVSDTHITL
                     SEVSLEEIQIQDLEESIKNNLSNLEGFTISKEEWTLTKDDKEPNYRSDKPYTWIALKF
                     PQRKDLFLELERITNELEINNNDEYIKNVKRIEEDIKDDECLANHMNLSNYTRREKAD
                     ECWNYFNNKLPNKITFDRIALRSEDGRLLLIIPLSQ"
     gene            complement(15281..15898)
                     /locus_tag="PKI16_02510"
     CDS             complement(15281..15898)
                     /locus_tag="PKI16_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53323.1"
                     /translation="MDISKLNLRPISEDKVVSIFLKGELKSKRFSKDIKDTIKELNTN
                     ISLIQNPNLNNAEENRLRLNIIDKTRGYTSRTKLFEDFPKDVKWYRTEVSKEFLLNEV
                     MYIDYDYWVGLTNGSRLPKDAVEKIQKNEQVFNVSYDNLLEASEYFKKNKSFDEIIIV
                     SDKKKFVVLEGHLRLTVYALNKEILPKENTVIIGISERMSEWSNF"
     gene            complement(16001..16672)
                     /locus_tag="PKI16_02515"
     CDS             complement(16001..16672)
                     /locus_tag="PKI16_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53324.1"
                     /translation="MSKVKNFNTTEDITISDLFQKLDTYLIGDIRAILNARHEDDSGV
                     GYPCLITILAGMELLGFLLSGDKDCAFTICWNELAKTNNKYKSDPLRKVFRQTIRNGI
                     AHNYLAKSGVYVHYSSTEKHLQQVRDKGVKGLCISCEQFFNDFLVVYEDIKGYLIQHP
                     EKAYLDELIGDLRSGQKYVDKYLNSLSFKSIDSGKVLTKKDLYGASGFGMEDGDTMDS
                     RYISA"
     gene            complement(16676..>19170)
                     /locus_tag="PKI16_02520"
     CDS             complement(16676..>19170)
                     /locus_tag="PKI16_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNR53325.1"
                     /translation="GKPKAPDYAFKVGSDRKFFVEAKASSVRVFSEIDPAFQLRRYAW
                     SGQLPVSILTDFEEFSIYDTTLEPKHTDKSSVARVKYINYKDYLKEADYLWDTFSKEA
                     VWKGSFDKFAKREKKGSQLVDKSFLKEIELWRDLIAKDVASNNNLNIYELNSVVQKFI
                     DRIIFLRIAEDRGVETYETLRSAVKSPDTYSQLLKIFNRADDKYNSSLFDLKKDGLST
                     RIKISDKTLDRILNNLYYPKSPYEFSVIGVDILGSVYEQFLGKVIRLTKGGNAVIEEK
                     PEVKKAGGVYYTPQYIVDYIVKNTVGELVKDRTPKDVAKLKIVDPACGSGSFLIGAYN
                     YLLNWHHKYYVENDVEKNLKAKKLFKDGEGNYFLSTQEKKDILLNNIHGVDIDPQAVE
                     VTKLSLALKMLEGENSETINAQYKLFADRILPDLSSNIKCGNSLIGSDYYSDKQITLL
                     GDEELRKVNAFDWDKEFPLIFRYGGFDAVIGNPPYIRNTSLDELQKDYFSKRYVTAEG
                     QYDIYILFNELAERLLKVSGKLGFIQPNKFLGSEYGLNLLLLFKKNFILNHIWNVSLE
                     KVFSNASVYPYVFVMTKSKKDQQEESNIHMFDLGVESCFEGFANQYKLYKRLKNILKN
                     TEPLGSHVKYIQRGVPNSKIDIVSKGEYKAYKSKDLSSPYFNYSPSSVITYKDSQYSQ
                     EKKSEFEEEMVMLPRTVLKLRASLKPREVHVLDRIYYIVKKENNFSNELLLAILNSEI
                     MDKFYEFYFGSTKVGGGYYDLKGSQIKNLPVPKNPDKNLVREIENLVNKYISLKRETY
                     DLKITSTQVLQIEKALNSLQEEINLSVDKLYN"
BASE COUNT         6041 a         3068 c         3302 g         6759 t
ORIGIN      
        1 actattcaaa gagaatctat gttacttgaa agaacccctg gggaagattg gttgtatgga
       61 ctaggtatag attttacgaa aacaacgaca actggtgttt acaaagtatt taaatggtgc
      121 tctccttttc ctgactatgg agatacaaaa acaaaatcaa aattaccagc atatgatcca
      181 gcagttaatt tgggaattgg aaggaatgga tatcttccaa cggatagaac cattggaacc
      241 tcatctagtt gtaatggcga aggtctcgct acttttccag gatatgatac atctatagat
      301 tttcctgaat cagaaataac aattgacaat ggaattggat atgttgtctt tgaatccatc
      361 tctggaagag cattctttta cgacgcaaat ggagaacttg taaattacca agcagatggt
      421 aaacaggcag aaaatgtctt taattttgtt ttaacaatag atcctcttgg agtcggaaaa
      481 actagacaat ttgtagtgaa taatttatca gggaaaatat catcattagt attatgacaa
      541 agcatataga gaaaaaatat gaagcactag gatttgtaga agctttgatt gctattatgg
      601 tagttggtgc atcttccgtt attcttatgc aaatagccgt aaatacaatg caaggtgtaa
      661 tacagaatga aaagatagat actatgactc aaattgcact cgaaggtgca gaaacggtta
      721 aagaaattgc aaaccaagag aaatttacag gggaagatct atttccaaat cttgcaggat
      781 gttatgttcc actatcagag gctggagtct ttactttcaa aaagggtctt gatggtaact
      841 tcttatactt agaagctgat gcaaacagag gagaatatgt ttcaaatgca gctattaatg
      901 aagaattctt tagaatttac tgctttgaat cttattctgt tggtgacaga tatgtatctg
      961 ggaatattat tgttggagag ctaaattcgg aaggaacaat aacaaaagga aataatgtta
     1021 aagattatta ttactttact gttgttaatc tatgataaac ctgaaaggaa ataaatacga
     1081 aggttttagt ttggtggaaa tgttaatcac cattgttatc ttgggtatgg ttatgctaat
     1141 tgcttctgtg accctaacca ccctaattaa aatatccaca gtatctagta acaaaacaag
     1201 agtaagaaat gagactgaat ttgttttaga gctagtaaga agaactgtga gaaattcaaa
     1261 tcccgctgat gtaatgattt acaactctct ggattctcga atgtatgatt cagaaaacgg
     1321 tgttgttagg aataatcctg agatagcaga tatctcgtct gtgtatgcag ttccactagg
     1381 tgaaaatgta acagggaacg aaatacactt cagaccgtat ggatatacca attggatatg
     1441 tattggcttt tttagggata tggatccaga aagtgaaatg ggatatgtca taaaaacctc
     1501 tacacaagat ctttggggta atgcacagaa atgtttcgat ggaacaactg aggattatcg
     1561 tatgtacacc atagtgctaa attctgaaaa tgtagatatt aattcattca atatcgcata
     1621 tacagaatct actggtggaa actacataat taggtttgat ctctctgctc aaccgattag
     1681 ttggtactta gcctcaagtg ctcctgtaaa ccgagaggta tttaggcagg cggtagttac
     1741 aaccgaaggt ataaactggt aatatatata agatggatat attttaatat taaattatta
     1801 gaatctgaat atgggtaaga caatattaat catcgaagat gaaaaagatt tgttagattc
     1861 atacaaagaa ttagtagaat ctgtaggctt taatagcatg gtagcaacgg atggttatca
     1921 aggactagac atacttgctt cacaattggg aaatattgat cttgtacttt tggatttaat
     1981 gatgccaggt gttgatggtt tggaggtatt gaaagcaatg aagacaaaca aagaaaagta
     2041 tggagatatt cctgttatta ttcttactaa tatgacaagc gatacggtca ttaaagaggc
     2101 ttttgatctt ggagcctcct cataccttgt aaagatagat ctggattatg aaggtcttgt
     2161 aaaagaaata gaaaagtatc ttggttaata ccaaatataa ttactttttg atattatata
     2221 taaactaatt agttaaaatc tgctcaaaat atgtcaaagt tgcccgatca tgtaggtata
     2281 gattttggta cacattctgt aaaagctgta gagcttaagg atattttctc tgaaactcca
     2341 gagcttgtta atttaggctc acaaattact cccaaaggag tgataaatag tgaggacaaa
     2401 ttagaccaga agaaacttgc agatgtactc aaaaagctat acgattcatc aaatattaag
     2461 aataaaaatg cagtaatggc acttccagag ttttctgttt ttacgagatt tttagaattt
     2521 ccaggtgtaa aaaaagaaga gcttaaggat gctgtatatt ttgaggctaa acagtatatc
     2581 cctatgcctc ttgaagaggt tcagttgagt tatgttccta tcggatttaa ccaagagaag
     2641 aacgccccta gagtacttct agttgctgct cctcttaaga tcatagatat ttacataaac
     2701 attgctacct tggctggttt ggagcttgta gccatcgaga cggagtctgt agctatgggt
     2761 agagcaatgt ataagtctac aaaaaagaaa aatcttgtta tgctggactt cggagcaaat
     2821 agtacagaca tgagtatact ctctgatgga tatttagtat tctctcaaag tattgcaatt
     2881 ggttcagact ctcttactca agccattatt aacaaattca gttttgaata tccaagagca
     2941 gaggagttta aaagaaatta cggtgttgtt gagaatgttc tagaaggaaa aatttacaat
     3001 gttatctccc ctattgttga atctatcatg gtagaggtaa gaagaggtgt agagttttac
     3061 aaaaacaaaa ctctttctgc atctcccaca gattttatcc ttaatggaga tggagcattg
     3121 cttccaggtt tgccagagta tgtttcaaag agtcttggag taacagctac aatagctatg
     3181 ccatggaatt ccataaagat tgacaataaa ttaaaagata taatagcaaa gagtggtcct
     3241 tcatactctg tagctattgg tttggcacta aaagatgaat aaggtcccaa tccaaaacaa
     3301 aaaaccagag gatgctcttc ttattaataa gaagaaagaa actcccatta aaaaagttac
     3361 ccctcctacc ccacccacaa aggagagtac aggaatcaat cttattccaa atctttctaa
     3421 agaagaggtt attaaagaaa cgaaaaagaa gactttaaat atgggtgcgg ttattagtct
     3481 aatggcttta gtcgttgttt ctatactagt agttggtttc aatattattt ctaaaataca
     3541 gttgaatagt gagaaagaaa aactatataa ctatgaacag agattaagct ccgtctctta
     3601 caagataact ggaaataacg agataagaga aaggatatct ttgtataaag acatccaaaa
     3661 aaatgctttc tctcctagaa ccgtcattga gtacataaac aatattgctt caaagagtgg
     3721 tagtataaaa cttagttctt ttgattttgg aagtcagctt tctttcgata tagatggaga
     3781 ggcttacagt tatgaagaag tctcgaagtt ttggtatctt ctttcaaacg atagtaaggt
     3841 tgagagtatt gtactaaaat ctgttgggaa gacagaaact ggagtgagat tcacttttca
     3901 aggaaaatta gtatttactg attttgtatc atcaacataa tggcaaactt aataaaaaag
     3961 aatgtaaaag aagaaaaaat aaagaaagag atagaggcag taagtgagaa gattcagaaa
     4021 actcgattga catttactga tatccttgtt ccgatagtag cagttatagt tctagttgta
     4081 cttggaatat ttgtatttat accaatgatt aaggaagctc ttgcagtaag gaatgaatat
     4141 gtagatgtta aggcgaagga aaaacaattg gaacaacttg agatagctct gaataatcta
     4201 gacgagacca ccctacaagc agatttggtt aatgcaaaaa atgttatacc aaagacttta
     4261 aaggtttcta gctttatata ctacatagat aatctggctt ttgagaaaaa tctttcttct
     4321 gagaaaatct ctgcaggaga cataaaaatt ggtggtggtg aaaagaaaga aggaaaatat
     4381 atattgggtg tgagtggacc acttgcatat tcgggtagtt tggaaaatat attgagtttt
     4441 ctagagagtt tgtattctgc atctccatat atcatatctg tagagaatat atccctgtca
     4501 gaatctagca aggattggac agtagagtta aatgtcacag gatattatgt tccagagaat
     4561 attgatgagt ttaatcttta cagtaaattc gagccatata caacggatga taatgtcgtt
     4621 aagatatttg aaagtaaggc taaagagcta gattagatct cgtcgtagag catatcccct
     4681 tctgtagaat ttccctcttg tggagcttct ttttgtggtt tttctctatt tccagctttg
     4741 tcatccttaa ttagaacttt tactcttcta tctgtacgaa ttgcattgat agtgattaga
     4801 tctcttattg cgttgatggt tcttcctttt ctacctataa caacgccttt atcttcctct
     4861 gcgacgtcta ttgtgagaat agtaagtcca ggaaaatcta cactttcctt ctcctcaata
     4921 cttacactat ctggatgatt tactattgac ttcacaatgt gttctagtag ctctttcata
     4981 tctgtaataa aataattaaa atactttaat tattatactc ttactcttgc tcctttgtct
     5041 tcttttctgt gttaggtttg tgagaacctc ttttaaggct cttaagtaag cctaacttaa
     5101 cgaaatactg tgcaacagtg tctgtaggtt gagctccatt ctttaaccat acagctgcct
     5161 tctcctgatc aatttcaaaa actttaggat tacttcttgg actgtaaacc ccaagctctt
     5221 ctattggtct tccatctctt cctttagagg attcaattac aacaattctg taatgtggat
     5281 ctcctcttct tcccgttctt ttaagtctta tttttaacat agtgatggat tttatcagat
     5341 gatatatatt aattcaagga ttcagattct tttcctatct tttcgatagc cgcaagagct
     5401 gcaccttctt cagctttttg cttacttgtt ccctgtcctt gtgctttttc cttgttatta
     5461 atgagaagag caactgtaaa tttcttgtca tgatctggtc cctcttcttt tacaattaca
     5521 taggttggag ttgttttgta ttttgcttga attatctctt gagcttgagt ttttggatct
     5581 atgaagagat tattctctac tattcggtca atctttggta ctaaagttct atttacaaag
     5641 ttcacacact cttccattcc actgtctaga tatattgctc ccagaacagc ttcgtatgca
     5701 tttgcaagta agtagtcttt gttttcacct ccagaatctt tctctccttt ggacatttgt
     5761 aaattttctc caacaccaag ctttctggat tcttctgcta aactttcagt tctaactact
     5821 gcagagcgaa tacttgttaa ttctccttct gcctttcctg gatactttcg aaatatatag
     5881 tcagagataa ttagttctaa tactgcatcg ccaagatatt caagcctttc attattctct
     5941 atttttgctc cttggtgttc gtttaagtat gacctatgag tgagagctat ctccagtaat
     6001 tgaggattct tgaatgtaat accaatattc ttttgtatat cgttaaactt ttccatttgt
     6061 ttcttcatat attgcaccta aaacaccatt tacaaattta ctacttgttt ctcctccaaa
     6121 ctctttggca atctcaattg cttcgtctag tgctactttt ggaggtgtta tcttacctat
     6181 aaatccctcg tatatcgcta ttctaagaat ttgaaggtca acaagtttta tctgatttaa
     6241 tggccactgt ggtgcatatt ttgtaatgat tgaatctatc tctgtgttat tatctataac
     6301 tccctcttta attttctcat ataattttgt gtcgtattct gtaatttcat ctaactcaat
     6361 taactctgta agaggaatag tatctgtatt aacgctatct ttgttgaaat cttttgagaa
     6421 aagttcctgt agtgccagga ctcttgctaa atgtctaggg tcagtcgctc ttttcattta
     6481 tttagtttct ttaatttctt tatagaagcc acaacttgga cattcaaaat gtgctctctt
     6541 catatttcca catttgggac atttagaaag agatgtaact ttcaatccat cttgagctct
     6601 tcttctgttc tttctccctt tggatattct tctttttggt agtgcaccca tggttaaaat
     6661 tcgtatatat taactttgag attatatggg ataactgcgt atttttcaac tttttatatt
     6721 atccaattct cctttatatc tatctcactc ccagacttga ggtttccttc aataatttcc
     6781 tttgcaatct gtccctctat tttatcttgt atatatcttt taagaggtct tgcacccatt
     6841 cctggctcgt tggctttgtt tgcgatgagc attggtatct ggtctgtcca tttaatattt
     6901 atacctttat ctatcattct ttctttaagt tctgttagaa gaagttctgc aacattccta
     6961 agctgattta tatctaatgg tgagaacaca atgactctgt caaaacgatt tagtaactct
     7021 ggacttagtg attgtctaag ctctatcatg gctctgtctt ttgcttctcc ccacagtgct
     7081 tcatcctgct ctagtgcctg aagaagaatt ccacttccaa tattacttgt acaaacgata
     7141 atagtattgg taaaatctac tgtttctccc gctgcacttg tgagtcttcc ttcatcgaaa
     7201 atctgaagga aaaggttgag gattttagga ttagctttct caatctcatc aaagagaact
     7261 actgtgtatg gattactctt taccctatct accaatgtaa tatttgtttg tccaaataca
     7321 tttcctgttt ctgttgtccc aaggaatttg tctacactat ctatatcttg gtactctgac
     7381 atatctactc taattatgtc actcttgttt ccaaaaaatt cttcccctac taccttcgca
     7441 agatatgttt ttcctacacc tgttggaccc ataaaaagaa agactccaat tggtttttcc
     7501 ggatttctaa tatctgttct tgcccttcga agagattccg tgatagcaat aactgcttcg
     7561 tcctgtccga tgattttttt ccgtatattc tcttctagtt ttcttaattt attactttct
     7621 tcttggtcta tatctgttat atttatattt ttctgtattg agatagcttt agaaacatgc
     7681 tctgctgtta tcttctggat gttatttgat tgtgcccatg agcatgttgc ttcaattgtc
     7741 tgtactgcac tacttgggag tttcctttcc ttgatatatc tgtaagctag ttctacagat
     7801 gcaactagtg ctgggaatgt aatgtagcaa ttaaaattct tttctaatgt tgcaatcttt
     7861 gtttccaaaa ttttcaatgt atctgtagta gaaacttctt taacctcaat atttgtgaag
     7921 ctttgtctta aggattcgtt actgtagaaa tattttttgt aatctgaata gtttacagtt
     7981 ccaattatgg ggaatttact gtttaggata tatggaagaa gaattcctgc taaagaatgg
     8041 ttagattctt cagctttagc tgggattagc tcttgcatct catctatgta gagaattgca
     8101 ttaccggaat tttctaattc ttgcattgcc ttcattatta gttcttcaat atttcccatt
     8161 tttagagagt gtgctattaa tccattgata tccaattgga caattcgttt gtcttttaat
     8221 tgtattggaa cattgccagt atttattctt tgagcaactc caagtattaa gctagatttt
     8281 cctactcctg cttcaccaat tagaagagca tttttgtttg aaagttttcc aaggactgaa
     8341 actagatttt caatttcttc atcatgtcct ataccgtaga tatctctgtt atttgctact
     8401 tcctcagtta aatctttact gaagtgtcct aatatgaagg tgtatccata tatccatctt
     8461 ctagctatgc cacctttgtt aaagtaaggg atatttggat caagatgttt ggttctttgt
     8521 gcttttgcaa ttagactgtt gttgtaaaga gagatttctc taagtatgtc tatggatatt
     8581 cctttcgctt gtatatactt gagagtctgt ggatacaatc ttagtatggc aagaaatata
     8641 tgttgaggtt ctaactgtct gctttctgtt aagatagctt cctctatggc gtatgttagt
     8701 atgcttctca gggctgtaat tggataatct ggaatgacat cctctccgat tttgaatttt
     8761 tcttccatct catttacaga catctctatc ccagatcttc ttacaatctg ctgaatgtcg
     8821 taatcctctt taagaagata tttaaaaaga tcgtatgttt ggaattggtt gtcttttgta
     8881 aagttacaga gtaattcaag agtttgttct gaagcaaaac ttacagtatc tagatgacca
     8941 tatcttccaa atttaaaccc tgttgctttt atctcatcta tctctttctg ttgtattttc
     9001 tttgcttttg gtaattgaat aggatggctt ttttcttcgt agaaatcatg ccagaggatt
     9061 atgacattga atatggaaat accaaacagt acatttacta acttactctc tagtatttca
     9121 gtaatggact caaattgatc caagagatca gcatatttta aaatagaggc tagagcaatg
     9181 aagacaccag gtagaacaag aatatttgct actattcttt taatatctcc aaactttttg
     9241 taccttaagt aatttttaat aacagatgta atctgtggtg tagatataga gaagaaatga
     9301 tctctggtta gaagaaatat ggagtcattc tcatcttgaa agattggaaa tttgttctca
     9361 aggataccaa atttattggt atttctttta tggtatccac atattgtttt ttcttgcata
     9421 tcaaaatcta atgccataac tatctaagta ttgaagtaaa aatgaatgta agtgtaccaa
     9481 tcggaagtag taaccaaatt agtatgatga ttgtgtaaac cagtatactt gtaagatata
     9541 tgattaaagc aattggtatg tacagaattt taatgagtag tccaaataca tatcccatca
     9601 ttgaataatc tctatgccat ggtaggaaga aattttgtac taacagagac agtgagagat
     9661 tgtcgttaac aacaacacta atacgagcaa gctttcttaa atgccatatt ggcattctta
     9721 cataccacca tcttaaaaaa tcttgtatga agtacaaaaa tatagccgaa tatgaatcgg
     9781 tttttgtctc tgtgagtaat tgttgtccgg aactatccga gactgtgttt ttaacttcca
     9841 ttttatgggc agtttatctt tatctttaag tctatttgaa atacttacag aattcaagtt
     9901 tttacaatat gtgatagaat tggcgatgga aggtaaatta ctaaaaatcc tcgctcctat
     9961 ggaggatgtc acagatactg tttttagaca aattctttgt gatattggaa aaccagatat
    10021 attctttact gagtttatga atgttaatgg ttactgttcg gagggtaaag atgttgttgc
    10081 aaagagaata tactttgaag gtatagagag gccaatcata gtacaacttt ggggaaactc
    10141 tcctggaaat ttcgcaagtg caacaaaaga aattgctgaa actgttaaac cagatggtat
    10201 agatatcaac atgggatgtt cagtaaggga tgttttaaaa actggtggag gttcagcctt
    10261 aatacaagat agaaatctta gtggtgaaat aatagatgca gtcaaagaga atgccagaaa
    10321 tatacctgtt tctgttaaaa caagaattgg atatgacagt gtagattact cttggatagg
    10381 gtttttactt tctaaaaaat tagcaatgtt aactgtgcat ggtaggttag caaaggaagg
    10441 ctactctact ccatctagat ggggtatttt caaaaagatt aaagagataa gagacgaaat
    10501 atctccgaat actttgttaa ttggaaatgg agatattaag aacgagaaag aaggaaatga
    10561 tctagtcatt aaatatggtt tagatgggta tatggtagga agaggtgtcc taacaaatcc
    10621 atggctgttt tctggaagag aagatatccc aaagaaagaa agagtgaaaa ccttaaaaaa
    10681 acatttaaaa atcttcgaaa agacatatgg tgagaatcag aatttcaata ctcagaagaa
    10741 atatataaag gcatacatca ataactttga cgatgctgga aaaattagag aagagctaat
    10801 gaatacaagt actcttgggg agattaaagt taaaatagcg agatatggaa attagtggac
    10861 aagagagaga atgtacatat agtgaggtaa tggttgaatc ttttgcagaa gctgtagatt
    10921 atcttaggaa agagacagat gaaaatgaaa gtaaattttg tttagtggat gttgatggta
    10981 ccctgtttac agacaatgtt ttaaagttgc ctgttctttg tcactttgtg cccctaacta
    11041 tatcagagga tattaagaat tctttttatt ccctaatgtc agatgttttt gtagacaata
    11101 atattgcagt aataacaaat aggaataatt ctgaaagaat attatggaat agtaacaagg
    11161 ttttacagca tgttaaagat gtttgtgagg ctccgttatt tacttctcta aatagacaaa
    11221 ttccaggttt aaacaagagg aattgtgata acttgctgag cagagtaatg gaatatgtgc
    11281 agagcaagga ttatctaaag ctatactcaa tagaggatca tagtttaatt tctcctttta
    11341 gaaatcattt tctggaatat cttgcaaaaa ggataaaata tgggagtggt acggatgtag
    11401 aggtagttaa tcttgtaata aaaagataat tgactagttt agtagtaatt gctaaattag
    11461 atatattaat gtaattctgc caaagtatga gtgattttga tccaacacaa ataccatctc
    11521 agtcctcaga tggggatatt ccaagctacg aacaaggtcc tcttccggga caatttacag
    11581 atttaaaaga ttcaaccgga ttatctgtag aattacaaga agtttttgac aagggtttgg
    11641 aatatttgaa agataatcct gtgctgagtg cagatgtcag aaggatggaa gaacgtgtcg
    11701 ttgaggttgt ggaatatcca gggaatgtaa atttatttgg aactacacga aagactttgg
    11761 ataaaggaga tttgaaacga gtgcatggtc ttgagagtta tgattttaaa agaaatggtc
    11821 cagattcgga aactttaaaa atttcagtta gaagtctaaa gaatactgct gctgcaactg
    11881 agtatgtaaa gcgaatagag gaagattatt ctatttacag tgagagagcg gatacaaagg
    11941 ttaattatgc tatgcagggt gttgaagaga gaagaaattt ggatgttagt ttttttgacc
    12001 aatccaggat atatagagac agagcaaggc aggaggcata caaaacaatt gaggatataa
    12061 ctttcttttt tgaagattca gaggagagaa ggatatgtgc gatggtgatg aagaagactt
    12121 tcggtggagt gtttgtatct ttaatagatt ttagaaatcc agacagagct aagatgtttg
    12181 caacagagac ccctgtatta ttagagaatt ataatgagaa gaaatttgat tctctaaaac
    12241 agaaattgtc tagatatgtt aatggtatga agagggatat ggattaattc actatgcttc
    12301 atattattgt taaaaccaat tccaaggata gtaaggttgt ttttgaaaac gatatttact
    12361 atgtatatac aaaatctcct gctaaagaag gtaaggcaaa tgatgaggta atatccctac
    12421 tctctgagta tttagatata cctaagggta atataaatat taagagagga aagaaatcta
    12481 ggagtaagtg ggtggagata gtttaagaga ttttaagctt tgagatgtga attattcggt
    12541 atttatatct gggccaatga agtaaaaata atcttggtaa ttgtgatcat tagtaaatct
    12601 attctcaata taatctgtag attcatctct agtcataacc tttaaacctt gtgtatcatt
    12661 gggttcaact aagacatact gattctcatc ccatttgtgg atagcattac agtgttgttg
    12721 agtcccacaa atcatacttc catccctgat agtgtccaaa tagtccataa gagattgagc
    12781 tttctctttt ctagattcag ggttttgtaa tcctagtgta agttggaatt cttcgttatt
    12841 tggattattt ccatatatta aagaaaagtt atcagtccat actttacgaa tagttccatt
    12901 atttattaat ccttggtcta tctcttttaa attcggaagt ttgccatgta ctaatgacca
    12961 taggttcata aaggtaaggg ctgcacatgg acctgattct gtttgataat aatattcata
    13021 agatttaggt ttacttccga ctattattgt tctttggagt tttacagcac cgttaagctc
    13081 tgatatcggg attattttat ctgtttcatt aggtccttca aagttgtctt ggttgttcat
    13141 atgggaaatt ataacacaag gaaatgtcga tgatattaaa tggcatggaa cttttaaaca
    13201 gaagaatcct gtatctactt cttcatgatt tatgttagat tatattaatg acagaaccaa
    13261 ctaatacaac cagagaggaa gaatctctaa aagatatcag attagagcca ggaatttatg
    13321 atcttactcc agaaataaga gattacatta caaatggaat tcccttatct gaagagacat
    13381 taaacgtatt gaaaacattt gatccagagt atgacttcgg aagagatata gaagagagag
    13441 gaaaagatgt tgttcttgca gatttagata aagcctctaa ggattttaat tttgaagagg
    13501 caaagcaagc agtacaagaa ggactaaaga tggcccagat tataaaccca gatataccca
    13561 ttaaaccatt cccggtaatt tttcttttta tcccccatta tggtgatgca aaagctttat
    13621 atggacaagg gtgtggaata aatatcggag cactaaaaaa taagagatat ggtgaagaat
    13681 ctccgagaca aaagattgtt tccttcacgg cccatgaagc tacacatact tttctaaaac
    13741 agcttggcaa acatccagaa cctggtaata gaacatggga aagggttgcc ttagatttta
    13801 tatgggaaga aggtttggct acatatgttg aacctacaca ttttctaccc catgatacgg
    13861 tagaggctga cggtgctttc tgggtcgata ttattaagag atggtatagc agagagaacg
    13921 atgaagatgc tcgtaagata tatgaagata ttaaagagag accgtctttt caagtatggt
    13981 acaactacat gtattataat cagccgatac caaaagattt aaatctttct gaggaaagtt
    14041 ttgaaacttt gttaaagaaa agaaatggaa ttggatatca tgttggttcg tatctttgga
    14101 agaaacagat agaaaaagga aaatctttaa aagatttagt tatgcaaggg agtcatggaa
    14161 tagatgaatg gatgaatgaa taatggatgt gaagctaagg attaaggatt ttaattgttt
    14221 cttccaagcc tttatattct tttatcatgt catcatctat ttctatccat ttgtatgctt
    14281 gagattcatt cgtgtctatt ttgagattag aaatatcatt aacgtttatt agatatgcaa
    14341 gaattaccca tctttgatcc tttgtgaagt gtatgagagg ttctgctgtt ttaacgattt
    14401 cgccatctaa attggtttcc tcttttactt ccctcaaagc tgtttcttct gctgattcat
    14461 gttcttttat atggccagta acgcaattcc aatatctggg attgaatcga ttatctgctc
    14521 tttgcaatac caggtattta tctttatgta caattagagc gtgcatggtt acaagagttc
    14581 tcataatgtc ttcttcataa attattggct caaaggtatt attaatagca gtctaccatc
    14641 ttcactcctt aatgctattc tatcaaaggt tattttattt ggaagtttat tattaaaata
    14701 attccaacat tcatcagctt tttctcttct tgtatagtta ctcaaattca tatgattagc
    14761 taagcattca tcatctttga tatcttcctc tattctcttt acattcttta tatattcatc
    14821 attgttatta atctctaatt catttgttat tctttctaat tctaagaata gatcttttct
    14881 ttgaggaaac tttaatgcta tccatgtata tggtttatca cttctgtagt ttggttcttt
    14941 gtcgtccttt gttaatgtcc attcctcttt tgatattgta aagccttcta gattggagag
    15001 attgtttttt attgattctt ctaaatcttg gatttgaatt tcttccaaag agacctctga
    15061 aagagtaata tgcgtgtcgg aaacagaaga tttgttgcta aatctgaaat tattctcata
    15121 gatatataac cgaagggaat tgagatattc tagatccttg tctgttggta tggctactat
    15181 gtgtgcaatc atgattaatt atacaggaaa tgaatgtaga atggtgggat gtgttggatg
    15241 gtatgggact taaaaatatt attactctat ttctattact ttagaaatta ctccactcac
    15301 tcattctttc tgagatacca ataattacag tattctcttt cggtagtatt tctttattta
    15361 atgcgtatac tgttaatctt aaatgtcctt ctaaaacaac aaatttcttt ttgtctgaaa
    15421 caataataat ctcatcaaat gatttgttct ttttaaaata ttctgaagct tctagaaggt
    15481 tatcatatga tacattaaaa acttgctcat tcttttgaat tttttctacc gcatctttag
    15541 gcaatctaga accatttgtt agcccaaccc aatagtcata atcaatatac attacttcat
    15601 ttaaaagaaa ttctttagat acttccgtac gataccattt aacatcctta ggaaagtcct
    15661 caaagagttt tgttctagaa gtgtaacccc ttgtcttatc aataatatta agtcttaatc
    15721 tgttttcctc tgcattattt aaattaggat tttgtataag agaaatattt gtatttaatt
    15781 ccttaatagt atccttaata tctttagaaa atctttttga tttaagttct cctttcaaaa
    15841 atatacttac tactttatct tcagaaatag gtctaagatt taattttgat atatccatag
    15901 ggaaattata acaggaaaaa gaaaatggtg ggctacactt tctggcatgg aacttaaaaa
    15961 taggatatat ttgcaatcat tcttgaaggg atgttagcta ttatgctgag atataccttg
    16021 aatccatagt atctccatct tccattccaa agccagatgc tccataaaga tccttttttg
    16081 ttaatacttt accactatca atacttttga aacttagaga gttaaggtat ttatctacat
    16141 atttttggcc acttctcaaa tctcctataa gttcatctaa gtaagccttc tctggatgtt
    16201 gaatcagata tccctttata tcctcatata ctaccaggaa atcattaaag aactgttcac
    16261 aactaataca caaacccttt actcctttat ccctaacttg ttgtagatgt ttctctgtag
    16321 agctgtagtg tacatataca cctgattttg caaggtagtt atgtgctatt ccattcctta
    16381 tagtttgtct aaatactttc ctaagtgggt cagatttata tttattgtta gtttttgcca
    16441 attcattcca gcaaatagta aatgcacaat ctttatcacc agataataga aatccaagta
    16501 gttccatacc tgctaaaatt gttattaaac aaggataacc tactccacta tcatcctcat
    16561 gtcttgcatt aagaattgct cttatgtctc caattagata agtatcaagc ttttggaaca
    16621 agtcagagat tgtaatatcc tctgtagtat taaaattttt tactttagac attttttagt
    16681 tatatagttt atctacagag agatttatct cttcctgtaa agaatttaga gctttctcaa
    16741 tttgtaaaac ttgagttgat gtaatcttta aatcataagt ctctctcttt agagaaatgt
    16801 acttattaac taaattctca atttctctaa ctaaattctt atcaggattc ttaggcacag
    16861 gtagattttt tatttgagag ccttttaaat cataatatcc acctccaacc tttgttgagc
    16921 caaagtaaaa ttcataaaat ttatccataa tctctgaatt taagattgct aggagaagtt
    16981 catttgaaaa attattttcc tttttaacta tgtaataaat tctatctaat acatggactt
    17041 ctcttggctt taaacttgct ctcaatttta aaactgttct tggaagcatc accatttctt
    17101 cctcaaactc agatttcttt tcttgagaat attgagagtc tttataagta attactgagc
    17161 tggggctgta attgaaatag ggggaagata aatctttgga cttatatgct ttgtattctc
    17221 ccttactaac tatatcaatc ttagaatttg gaacacctct ttgtatatac ttaacatgag
    17281 atcccaacgg ctctgtattt tttaatatat tctttaatct tttatatagc ttgtattgat
    17341 ttgcaaagcc ttcaaaacaa ctctcaacac ctaggtcaaa catatggata ttagattctt
    17401 cttgctgatc ttttttactc ttagtcatca caaaaacata ggggtatacg gaagcattac
    17461 taaagacctt ttctaaagat acattccaaa tatgattaag aataaagttc ttcttaaaaa
    17521 gaagtaataa atttaatcca tattctgatc caaggaactt gttaggctga ataaaaccaa
    17581 gcttacctga tactttcaaa agcctttctg ctaattcatt gaataaaatg taaatatcat
    17641 actgtccttc tgcagtaaca tacctctttg aaaagtagtc tttctggagt tcatctaatg
    17701 atgtatttct aatatatgga ggattaccta ttacagcatc aaatccacca tatctaaata
    17761 ttaatggaaa ctccttatcc caatcaaaag cattaacctt tctaagttct tcatcaccaa
    17821 gtaatgttat ctgtttatca gagtaataat cactacctat tagactattc ccacacttaa
    17881 tattagaact taaatcagga agtattctgt ctgcaaataa cttatactgt gcatttatag
    17941 tctcactatt ctctccctct aacatcttta aagctaaact aagctttgtt acttctacag
    18001 cctgtgggtc tatatctaca ccatgaatat tgtttagtaa gatatctttc ttctcttgag
    18061 tagataaaaa gtaattacct tctccatctt taaataattt cttagctttt agattcttct
    18121 ctacatcatt ctctacatag tatttatgat gccagttcaa aaggtaattg taagcaccaa
    18181 ttaaaaaact accactacca caagcaggat ctactatctt taacttagca acatcttttg
    18241 gagttctatc tttaactagt tcccctactg tattctttac tatgtaatca acaatatatt
    18301 gaggtgtgta atatactcca cctgctttct taacctctgg tttctcttct attacagcat
    18361 taccaccctt ggtaagtcta attaccttcc ctaagaactg ttcatataca ctacctagaa
    18421 tatctactcc tattacagag aactcatatg gagatttagg atagtaaaga ttattcaata
    18481 tcctgtctaa tgttttgtca gagattttaa tccttgtaga tagcccatcc ttctttaaat
    18541 caaataaact actgttgtat ttgtcatctg ctctattaaa gatctttaag agttgtgaat
    18601 atgtgtctgg agactttact gcacttctaa gagtctcata ggtctctaca cctctatctt
    18661 cagctatcct taggaagatt attctatcaa tgaatttttg tacaacagag tttagttcat
    18721 agatgttcaa attgttatta ctagctacat cctttgcaat taaatctctc caaagttcta
    18781 tctcttttag gaatgatttg tctactaatt gacttccttt cttttctctt ttagcaaact
    18841 tatcaaagga tcctttccat acagcttctt tagagaatgt atcccaaaga taatctgctt
    18901 ccttaagata gtctttgtag ttaatatact taactcttgc tacagatgac ttatctgtat
    18961 gctttggttc taatgttgtg tcatatatgg agaactcttc aaagtctgta agtattgata
    19021 ctggcaattg accactccat gcatatcttc ttaattggaa tgctggatct atttctgaga
    19081 atactcttac tgaggatgct tttgcttcta caaagaactt tctatcacta cctactttaa
    19141 atgcataatc tggtgcttta ggcttacctt
//
LOCUS       DAOQWP010000015        17447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_3644_length_17447_cov_7.196125, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000015 DAOQWP010000000
VERSION     DAOQWP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3644_length_17447_cov_7.196125"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PKI16_02525"
     CDS             <1..972
                     /locus_tag="PKI16_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNR53326.1"
                     /translation="EVYSKVYGGLGIYGKPMNANARKNKGLDFNSINRMLSSLRSYLK
                     FRISWDLDFPLAPDAIEMSKSDKRVKKVASLKDLTRLIESPMEFEKDEKVAIRNRCML
                     EVLFATGLRISELIGLDIEKINSEGKMYIVGKGKKERAVFLTGRSLDWLNKYLRIRLK
                     YAFTDRDEKNQPVEMDVLIKEKENTERETYSGNDVNLEMFDKGNRKYIEMVEGYRKSG
                     YLKKFDSPALFIPFSGGRAGKPNARISTNYFQEKIAEYRKKLGIQIPTSAHSLRHGYA
                     TYMAEDGASPVALQALLGHESLNTTSKYVHASEKFAEDTVKKNHPLK"
     gene            complement(978..1403)
                     /locus_tag="PKI16_02530"
     CDS             complement(978..1403)
                     /locus_tag="PKI16_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53327.1"
                     /translation="MDYTTDIENIKNELKAIGFDDMKLNQLMDIAAEEILQMAIDDLG
                     ESADDATLEMVAQEVDKPITTAEEAVSRMSLIFEKAYGAEAENKKNEMLLNYLKQTLE
                     ATKSAKDLYNRYQAGDPSAVATIKAQEGNPDVQKVVDLM"
     gene            complement(1426..>2883)
                     /locus_tag="PKI16_02535"
     CDS             complement(1426..>2883)
                     /locus_tag="PKI16_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53328.1"
                     /translation="PIQHDETKMPELTGSWESQDNNQAEVDLSKFNETLTPPTNTVTE
                     NWDKNMSNSPSIVVATEKQTPVTPNVETRDLHQEALDSNKKANEGFVSGPALSNTGRT
                     FFNSEDIYAEKANLEEQNRMLMERLDKMQATLDRLTYEQDPALAAALDPNRPKSKDQL
                     EVDQAYATINKDLNQKALNEMAQVKAMLESKPSVTPEELITTPETQKKQRDFKTAALI
                     AGVVAGGAAGILGGTTVGIPVAAAAGLVSIGGRVTNSFLNRSLKNIEAQIQDPTLDVT
                     TKEKLQKRESSLKKIQNIVQHVTKFATGAAIGAGISTFISNTAMGGHGLVWNKPEVPG
                     GIPGGAEGSLGSTGVEQTQPTSTGETPLPASPETYPGNSFIHDGRVDLPGSAWDGNMA
                     AGPAQDTLAGGAENFANYAGGPSEMAANQLGQDLASNNITDQLLSNLTTYDKHRLLTE
                     YWNAIRAGNTNPELIETLKNINTDGAQKLLAAIGK"
     assembly_gap    2884..2983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3100..5046)
                     /locus_tag="PKI16_02540"
     CDS             complement(3100..5046)
                     /locus_tag="PKI16_02540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR53329.1"
                     /translation="MKELFLKNKKEYDFISISHYFYPRVGGLENMAFNLISGLQEKGL
                     NSIALYGANKRESSTINGFQAESFPVLNIFNGTYPIFGIRFALYTLKLLQENPNAQVV
                     VHSRHLTSSLITAMLCILTGHQYTVVEHNAGPVYFKSELTNKLAQWADRNIFSLVHKF
                     ATNTVTVSKAGRKWVSKTFDVDQSLINIIYNGYNSKEIKNSISKKENIVIWAAKWIKV
                     KDPSTALRAYKKIANKYPNWKFVLIGEGNALKEQKLLPENVEILPEFIKQEDLFRLLK
                     KSRIYINSSLSEGLSLGILEASAFGNIPVISDAPSNKEIAKEIGTSSYVFKRGNSFDL
                     ANKIEDAIANCSNTNTLKQIIKNTNIKFNNNTMVKNYYSYLYPEHLKAETFSTLSIVI
                     PVFNEERSILSLLEKVIEVKLPRNIRKEIVIVNDCSTDETKTLLESYIRKNKRYAQFK
                     LLENEINLGKSQSVKHGILHTTGDLVVVQDADLEYNPNDLSTFVETFLQYPNTDVIYG
                     NRFNRENIFINPTHYLGNKFVTLMSNIFTYGKGLVVKDMETCYKMAKGDIFRDLISTL
                     ESTTNFGIEPEVTAKFANWKKEDGKKLNLKQLDIHYKPRGQEDGKKMRWFKHGFEALK
                     EIAYFNTQKKIFSQKQEAIPSLRV"
     gene            complement(5142..7043)
                     /locus_tag="PKI16_02545"
     CDS             complement(5142..7043)
                     /locus_tag="PKI16_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53330.1"
                     /translation="MRKRILITTLIIASYIYCLYFIFFKQTVGLSDDLIFAIISTSSI
                     FLLPAIISKLITKKSYFGIGYILATLIYPLSYLLIYFLSKQFNIESQTLYRALAYLFL
                     TFTSLSLIFLLFKKDIQNYANIKKSLVIYLGTFALLIGVYTLLGIKTDALLSTDFLQH
                     NAVANEMANGQLCLTPNQCSNLFQKLGYTTYFHSIQAFVTTGFNLNVGIASTIFNFSL
                     IATFAVLIAKILNRYFKNKFLILLGTLMTITVFEAGAYSFAFIIPQTFTLFLFLNLLS
                     ERKLNLASLLFAIPILLANHFIFGPIFAAFALIYYLFQKTLKKDSNTLKVISMVSVLG
                     LIVTLLANIRGFSIEKFFQLSEVEILGSFSNYYFPQNLSFLSTQYGFLIVFFIICAIY
                     IFIKKKSISFAYYSITYVSICLIFFFLAPTYASKFLIGTSLFMSFLLVYTINNLKFKK
                     WLSTIIIFILFLSSIPFYITNFKSYLTFYTQNTGVISGITEDDTSIIHFLSQKKATKC
                     QIVSDPYTQLMVRGMTLFETAQGQYQSLDTRRSMITFVSNPNNKTYEEMIVRDEISNR
                     FCFLYTSRIESASRDINPQNVAWLNNMYDYEINNNYGVSNLKLIELLSNKGFHIVYSD
                     SNNILFSRE"
     gene            complement(7040..7633)
                     /locus_tag="PKI16_02550"
     CDS             complement(7040..7633)
                     /locus_tag="PKI16_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53331.1"
                     /translation="MRKLFKIFFLIFALLSFGISLLYGTRIFRTPYIYEYINNSLVVT
                     IPNKIWFEINNQQDFLSKDNKPTYVFENDLLEYTKSGYNIGYIYPTYTSKTENDTITK
                     TDIVTRGKDTLEIKRSVKIQNHTPYDSYISKISFSKYGTFLDNTYTQDGCDISVSSKS
                     GELIYDQKTATLSISYNPDEVGEIEDTINISVQCKGL"
     gene            complement(7763..8371)
                     /locus_tag="PKI16_02555"
     CDS             complement(7763..8371)
                     /locus_tag="PKI16_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53332.1"
                     /translation="MSITEKDIKKISINIEMNESAKANAPVAPKATAPVAEDKIETKG
                     DSNLFWKVLGILGLLALLALIAIMLLNKPAVSAQTPGSAPIVANPVLPNNGTLESPDP
                     RSAGSNANFIGYFGSRGKDWVVPNQVFDTSGTTRDVGSVLFTWCDKEAICFYQILHPG
                     DVIQIGHQGSQINIYDKAYSGQETKELQYIATKENTSWTFVQ"
     gene            complement(8750..8968)
                     /locus_tag="PKI16_02560"
     CDS             complement(8750..8968)
                     /locus_tag="PKI16_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53333.1"
                     /translation="MKNTFSVLATSLGFILLNTSSILADGYGHIPVDTSFILDNKSIF
                     AGIALIVFGLGIGLMLEGRYLKSKTKAQ"
     gene            complement(9061..9741)
                     /locus_tag="PKI16_02565"
     CDS             complement(9061..9741)
                     /locus_tag="PKI16_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNR53334.1"
                     /translation="MKREKIINTTGNIYIIIGAILTFASLSLLAYPQLPYIFNKLNIS
                     SPEVEAETLQIPVTENKEDDTEQPPEPPQITLPEKDTSLTETNTLIIPKIGVKAKIIT
                     GKDPLKALDKGPWIVYDYANPEQNYLGETTKSIIIASHRFGYSSWTTEYRNKISFFNL
                     PNTKIGDKVIIIWNQREYIYQIFKLDENKYIKDIDADLILYTCKYYNSPIRIFRFANL
                     VSINGEPV"
     gene            complement(9747..10553)
                     /locus_tag="PKI16_02570"
     CDS             complement(9747..10553)
                     /locus_tag="PKI16_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNR53335.1"
                     /translation="MTEFFSNIWIYLSTLVLSFNVVTTDFQDTQSVEKNNVKTSNTIE
                     VNDIKESIIPKTYSNYTPSVSWWTYPSNIKTVKRNGNDLLVHVNKEYKLPSTYAPNDL
                     VKASNSGIRNGDNFYVRNILINDLRDLVNAAKADGIDLSIVSGYRSYATQVSTYNYWV
                     QYNGGNVDAADRISARAGHSQHQLGTAIDFSSKEVSDNIGTRFSTTKASAWLQKNAHK
                     YGFVLSFPSGYEHVTGFSYESWHYRYIGKTNALEMKNRGMILEEFLRNKN"
     gene            11145..11585
                     /locus_tag="PKI16_02575"
     CDS             11145..11585
                     /locus_tag="PKI16_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53336.1"
                     /translation="MLTKEDLKAIEKILSPRFDTLDKRMDKLEERMDKLEKKMVELEE
                     KLNFLEERLEKTYEELDRRICSIVDTVGEINGTLQGLALLDANSRGDIKRQEGNIQEV
                     YKMNIEVNDRLDAIEKHLNTKYDPVSRSLMDKSKIEYERKKLKG"
     gene            complement(11575..14196)
                     /locus_tag="PKI16_02580"
     CDS             complement(11575..14196)
                     /locus_tag="PKI16_02580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNR53337.1"
                     /translation="MKSNYLDYSMSVIVSRALPEVKDGLKPSQRRILVAMNDLALTPT
                     AHYRKCAKIAGDTSGNYHPHGESVVYPTLVKLAQDFSTRYVLVDGQGNFGSIDGDGAA
                     AMRYTEARMGKITIELLRDLNKGTVTFTQNYDGTRLEPTVLPALFPNVLANGTDGIAV
                     GMATKMPPHNLRELITALQDMIKRGNKWEGKGIYNELRKAKEAKEIIPQTLNSKPEDY
                     LENYVDLADPNLVKNIEDLRKKIIEEKSILYPEFKSDITPLDLIEIIPGPDFPTGGII
                     YDQKEILNAYATGRGRILQRAKASIVEGKQGRFNIIITEIPFQVNKAHMIEKFADLVK
                     EKKIDGIADIRDESNREGMRVVVVLKRDAQPKAVLNKLYKYTEMQKVFNANMIALVEQ
                     EPKTLPLKRILELFLSFRLTVTIRRYEFDLAEARFRAHILEGLLKALDLLDEVIATIR
                     GSKTQETAKTNLMDNFKFTEVQAQAILDMQLRRLAALERMKLQNEYKELQDNIKEYNA
                     VLENHDRILEIISKDLDEIKEKYGDDRKTKVIKGQVNEISEEDMVANEEMLVTITHSG
                     YIKRLTPDTYKSQRRGGKGVSGGETKEGDFIEHAFLCNTHDELLLFTNRGRVFNIRIF
                     EIPEYGRTAKGLPLVNLVQLEQNETINSVLTRNPNGTITEKTEGGESINYSYFLMATE
                     KGVIKKTALEEYKKIRSNGLTAIKLEDGDQLTWVKPTTGNNNVVIVTADGKSVRFKES
                     DVRPMGRSTKGVTAIKFKNATDYVVGMGIIKDDNEKLLTLSDNGYGKMTTLKEYPLQK
                     RGGTGVFTFRVTPKTGRIASARIIEKGEEEIVVISQKAKVIRSELKAVPTLGRQTSGV
                     KLMNMNEDDSVATVAIL"
     gene            complement(14311..14724)
                     /locus_tag="PKI16_02585"
     CDS             complement(14311..14724)
                     /locus_tag="PKI16_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53338.1"
                     /translation="MSNIFSGIKEVSDMNTPESEEFKQLLNMKVATELTKYHSAIFAT
                     IMEDPNEDLKEIFKEELKENQGICLEDNNSEKLLRANVLDKENKRIFFIDMSTRDEEG
                     LEKITQETYDALSEYVQDPILVMQNVKEALQDNAI"
     gene            complement(14742..16652)
                     /locus_tag="PKI16_02590"
     CDS             complement(14742..16652)
                     /locus_tag="PKI16_02590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HNR53339.1"
                     /translation="MPNKSDYGASNIQVLKGLEAVRKRPAMYIGSTDKNGLHHIFTEI
                     LDNSVDEAIAGYCSNIWVIFNKDHSLTVRDNGRGIPVAIHPETKVSTLETVMTNLHAG
                     GKFDDGAYKVSGGLHGVGMKCTNALSAQMTTTVRRDGGIFQQVYERGVPKAPVKRIGD
                     SNETGTEHTFLPDPTIFQETEFEFNTIVRSCRQHAYLTAGLRISVIDEREEKHTRYDL
                     YFEDGIKSYVQFLNIDEKSIGEDPFYMNKEDAGVLVEVSLQYTDGMEEDVRCYTNNII
                     NPEGGTHLAGFRTALTSAFNTYAQKAELLKGLNGTLNGDDVREGLTAVISVKVPNPQF
                     EGQTKIKLNNPEVKSIVGKLVRDELLVYLEEHPNEGKNIIGKAVLANKAKAAAKAARD
                     AVIRKGVLESTSLPGKLADCSSKDPSESELFIVEGDSAGGSAKQGRDRHTQAILPLRG
                     KIMNTQKYRVDRVLSNNEFNDVTTALGVGIGDTLDVAKLRYHKVVIMSDADVDGLHIT
                     TLVLTLLFRFFRPLIEQGYIYVAQPPLHKVEIGKKKYYFLNDAEKEAFVKEAKKEGKV
                     PVENRFKGLGEMNPEQLWETTMNPETRVLKRVEIQDAEEVEKTFEMLMGNEVPPRRRF
                     IQMYARKATLDI"
     gene            complement(16725..>17447)
                     /locus_tag="PKI16_02595"
     CDS             complement(16725..>17447)
                     /locus_tag="PKI16_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53340.1"
                     /translation="LLQSIFIYMIESDMTYLLINNNQKILEDNLVLLVNRFLKTNFVE
                     LEELFGVPDIHIVDGRQQNSIGIDEIKALQNEMIFQPFNNASQLAIILDSHKLTTQAQ
                     NSFLKTLEESSDNTIYILCVNNERNLLQTIISRGKLIYAKGDVEKKEEVTSDILELDL
                     VEQFLLVEKISKSKEDSLLFIENIENIFKKKLEFEIKNGKIESPKRLKNALDLLNDSK
                     KKIDSNCNKKLVLEALIVSLNT"
BASE COUNT         4976 a         3005 c         3037 g         6329 t
ORIGIN      
        1 gaggtttaca gtaaggttta cggtggtctt ggaatttatg gaaaaccaat gaatgcgaat
       61 gcaagaaaaa ataagggttt ggattttaat agcataaaca gaatgctttc gtctttaaga
      121 tcgtatttga aatttagaat atcttgggat ttagattttc ctcttgcacc agacgcgatt
      181 gaaatgtcca agagtgacaa gcgtgtaaag aaggttgcat cactaaaaga tttaacaagg
      241 ttaatagaat ctcctatgga atttgagaag gatgaaaagg tagccataag gaatagatgt
      301 atgctggaag tgctttttgc aacaggtctt cgtatatcag agctcattgg tttggatatt
      361 gagaagataa atagcgaggg taaaatgtat atcgttggta aaggaaagaa ggaacgggcg
      421 gtatttttaa caggtagatc tctagattgg ttaaacaaat atttaaggat aaggttaaaa
      481 tatgctttca cggacaggga tgaaaaaaac cagcctgtag agatggatgt tttaatcaag
      541 gagaaagaaa atactgagcg tgaaacatat tcgggtaatg atgtaaattt ggagatgttt
      601 gacaagggaa acagaaaata tattgagatg gtggaggggt ataggaagag tggatattta
      661 aaaaagttcg actcccctgc ccttttcata cctttctctg gtggtagagc tggaaaaccg
      721 aatgcaagga tatctacaaa ctattttcag gaaaagatag cggaatatag gaagaaatta
      781 ggtatacaga tacctacatc tgcacatagt ttaaggcatg gttatgctac atatatggct
      841 gaagatggtg caagtccagt agcactacaa gcgcttcttg gccatgagag tttaaataca
      901 acaagtaaat atgtacatgc aagtgagaag ttcgccgagg atactgttaa gaagaatcat
      961 ccgttgaaat aggatgatta catcaaatct acaacctttt gtacatcagg attaccctct
     1021 tgtgctttta ttgttgctac tgcactagga tctcctgctt ggtatctgtt gtataagtct
     1081 ttggcactct ttgtagcctc taatgtttgt ttgagatagt ttagtagcat ttcatttttc
     1141 ttgttttctg cttctgcacc atatgccttt tcaaagatta gagacattct agaaactgct
     1201 tcctcggcag ttgtaatcgg tttatccacc tcttgtgcta ccatttctag cgtggcatca
     1261 tctgcgcttt cgccaagatc atcgattgcc atttgtaata tctcttctgc agctatatcc
     1321 atgagctgat ttaacttcat atcatcaaaa ccaattgctt ttaattcatt tttaatattt
     1381 tctatgtcag tagtgtaatc cattggtata tatttttaaa ttaagttact tcccgatagc
     1441 agccaaaagc ttttgcgctc catcggtatt tatattctta agagtttcaa ttagttcggg
     1501 gtttgtatta ccagctctaa tggcattcca atactctgta agtaatctat gtttgtcgta
     1561 tgttgttaag tttgaaagta attgatcggt tatgttgttt gaagctaaat cttgaccaag
     1621 ttggttagct gccatctcgg aaggaccacc tgcgtagtta gcgaagttct ctgctcctcc
     1681 tgctaaagta tcttgtgctg gaccagctgc catatttcca tcccatgcac tacctggaag
     1741 atctactctg ccatcatgaa tgaatgaatt acctgggtat gtctcaggtg atgctggaag
     1801 aggagtctct cctgttgatg ttggttgagt ttgctctacg cctgtgctac ctaaactacc
     1861 ctctgctcct ccaggaattc ctcctggtac ctctggctta ttccaaacta gtccatgacc
     1921 acccattgct gtattactta tgaaagttga gataccagct cctattgcag ctcccgtagc
     1981 aaacttagtg acatgttgaa caatattctg aatctttttt agacttgatt ctcttttttg
     2041 taacttctct tttgttgtaa catcaagtgt aggatcttgt atctgtgctt ctatgttctt
     2101 gaggcttctg tttaagaagg agtttgtaac tctacctcca attgatacta aacctgctgc
     2161 cgctgcaact ggtattccaa ctgtagtccc tccaagtatg cctgctgctc caccagctac
     2221 tacgccggca attaaagctg cggttttaaa gtctctttgc tttttctgtg tctctggagt
     2281 ggttattaac tcttctggtg taacggaagg tttagactct aacattgctt ttacttgagc
     2341 catttcattt aaagctttct gatttaggtc tttgtttatt gttgcatatg cctgatcaac
     2401 ttctagctga tcttttgatt ttggtctgtt tggatctaaa gctgcagcta atgctggatc
     2461 ttgttcatat gttaatcgat ctaatgttgc ttgcatctta tcgagtcttt ccattaacat
     2521 tctgttctgt tcctctaggt ttgccttctc tgcatagatg tcttcgctat taaagaatgt
     2581 tctccccgta ttcgaaagag caggaccaga tacaaaccct tcatttgctt ttttattact
     2641 atccaaggct tcttgatgaa gatctctagt ctctacattt ggtgtaacag gagtttgttt
     2701 ctctgtagca acgacaatgc ttggagaatt agacatattt ttgtcccagt tctcagttac
     2761 agtattggta ggtggtgtca atgtctcatt gaatttgctc aaatctactt ctgcttgatt
     2821 gttatcttga gattcccatg acccagtcaa ctctggcatc ttagtttcat cgtgttgaat
     2881 aggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtttatt taggttatct
     3001 tattgaccta cctattttaa acatggagtt ataaataggc agatttatat atatccagaa
     3061 tataatggag atatgtcaat gtcaagaaat taagaggcgt tagactctta aagaaggaat
     3121 agcttcctgt ttttgtgaga atattttctt ttgtgtatta aaatatgcaa tctcttttaa
     3181 tgcttcgaac ccatgtttaa accatctcat ctttttacca tcctcttgtc ctcttggttt
     3241 gtaatgtata tctaattgtt tgagatttaa tttcttacca tcttcttttt tccaattagc
     3301 aaattttgct gtaacctcag gttctatacc aaagtttgta gtagattcta aagtagatat
     3361 taaatctcta aagatatctc cctttgccat cttgtaacag gtctccatat ctttaacaac
     3421 tagacctttc ccgtaggtaa agatattgga cattaatgta acaaatttgt ttcctaagta
     3481 atgggttgga tttatgaaaa tattctctct attgaatcta ttcccatata ttacatctgt
     3541 atttggatac tgcaggaaag tttcaacaaa agttgaaaga tcgtttggat tgtactccaa
     3601 gtcagcatcc tgaacgacta ccaaatctcc agtggtatgt aagattccgt gctttacact
     3661 ctgtgatttc cctaagttaa tttcattctc taacagtttg aattgtgcat atctcttatt
     3721 cttccttata tagctctcaa gaagagtctt tgtttcatct gttgaacagt catttacaat
     3781 tacaatctct ttccttatat ttcttggtaa ttttacttct ataacttttt caagtaggga
     3841 aaggatagat ctttcctcat taaatacagg gataacaatt gagagtgttg aaaaagtttc
     3901 tgcctttaga tgctctggat agaggtagct gtagtagttt tttaccatag tattattatt
     3961 aaatttgata tttgtatttt taataatctg ctttagggta tttgtatttg aacaattagc
     4021 gatagcatct tcaatcttgt ttgcaaggtc aaaactgttt cctcttttga atacataact
     4081 tgaagtacca atctccttag caatttcttt gttacttggt gcatcagata ttactggaat
     4141 atttccaaaa gcagatgcct ctaggatacc caaagaaagg ccttcactta aagatgagtt
     4201 aatgtatatt ctagatttct ttaacaatct aaagagatcc tcctgtttta tgaattcagg
     4261 taatatctca acattttcag gaagtagctt ttgttctttt agagcattcc cctcccctat
     4321 gagtacaaat ttccagtttg gatatttatt tgctattttt ttgtacgctc taagagctgt
     4381 acttggatct ttgactttaa tccattttgc tgcccagatt acgatatttt cttttttaga
     4441 aatagagttc ttaatctctt tgctgttgta gccgttatat attatattta tcaaagattg
     4501 gtccacatca aaggttttag acacccattt tcttccagct tttgatacgg ttacggtatt
     4561 tgttgcaaat ttatggacta gagagaaaat atttctgtct gcccattgtg ctagtttatt
     4621 tgttaattct gatttgaagt atacaggtcc tgcattatgt tcaactacag tatattgatg
     4681 acctgttaga atacaaagca ttgcagttat taaactactt gtgagatgtc tactatgtac
     4741 tactacttgt gcatttgggt tttcttgaag aagtttaagt gtatacagtg caaaccttat
     4801 accaaagatt ggatatgtac cattaaatat gtttaataca gggaaacttt cagcttgaaa
     4861 accattaata gtacttgatt cccttttgtt tgcaccatat aaagcgatgc tgtttaatcc
     4921 cttctcttgt aatcctgaga ttaagttgaa agccatgttc tctaaaccgc ccactcttgg
     4981 atagaagtaa tgggatatgg atataaagtc atattctttt ttgtttttca aaaaaagttc
     5041 tttcatttaa aaaggcttta attaaaaggg tttctattgc tagaaactag ttaattatat
     5101 cacgaaatag ccctatagta aaacacactg agaaggtcac cctactctct tgagaagaga
     5161 atattgtttg aatctgaata gacaatatga aagcctttgt ttgaaagaag ttctatcaat
     5221 tttagattag acacgccata attattattg atctcataat catacatatt gtttagccaa
     5281 gctacatttt gaggattaat atctctggaa gcagactcaa tcctagatgt gtaaaggaag
     5341 cagaatctgt tacttatctc gtctcgtact atcatttcct catatgtttt gttgtttgga
     5401 ttacttacaa aagtaatcat acttcttctt gtatctaaac tttggtactg tccctgtgca
     5461 gtttcaaaca gagtcatgcc tctaaccata agttgtgtgt atggatcaga aacaatctgg
     5521 cattttgtgg ctttcttttg tgataggaaa tgaattatag aggtatcatc ctctgttatg
     5581 ccagagataa ctccagtatt ttgtgtatag aaggtaaggt agcttttgaa atttgtaatg
     5641 tagaaaggaa ttgaagaaag gaaaaggata aagattatta ttgttgaaag ccattttttg
     5701 aattttagat tgttaattgt gtataccaat agaaatgaca taaacagaga agttcctatg
     5761 aggaacttac tggcatatgt tggagccaaa aagaagaata tcaggcatat agaaacatat
     5821 gttatggaat agtaagcaaa ggagatagat ttcttcttaa taaatatgta gatagcacag
     5881 ataataaaga atacaatcaa aaatccatat tgtgtagaca ggaaggagag gttctgtggg
     5941 aagtaatagt tagagaaact tccaagaatt tccacttcag acagttgaaa aaatttctca
     6001 atactaaatc cacgaatatt tgcaagtaag gttacgatta aacccagtac agataccatg
     6061 gatataacct taagggtgtt actatccttt ttcaaagttt tctggaagag atagtaaatt
     6121 aatgcaaatg cagcaaagat tggtccgaag ataaaatgat tggctagcaa gattggaatt
     6181 gcaaatagaa gtgatgctag attcaatttc ctctcggaaa gaagatttaa gaaaagaaat
     6241 agtgtaaatg tctgaggaat aataaatgca aaggaatatg ctcctgcctc aaatacggta
     6301 atggtcatta atgtgccaag taggatgaga aacttattct tgaaatatct gtttaaaatt
     6361 tttgctatca aaacagcaaa tgttgcaatt aaagagaagt taaagattgt agatgctatt
     6421 cctacattaa gattaaaacc agttgttaca aaggcttgta tagaatgaaa atatgtagtg
     6481 tatcctaatt tctggaaaag attggaacat tggtttgggg ttaaacataa ctgtccattg
     6541 gccatttcgt tagctacggc attgtgctga aggaaatctg tactaaggag tgcatctgtc
     6601 tttattccga gtaatgtata tactccaatg agtagtgcaa aggttccaag atatattact
     6661 agggattttt tgatattggc ataattttga atgtctttct tgaatagtag gaatattagg
     6721 gagagggaag taaatgtaag aaagaggtat gctagtgctc tgtaaagggt ttgagattca
     6781 atgttaaact gtttggagag gaagtaaatt aaaagataac taagtggata tattaatgtt
     6841 gctaatatat atccgatacc aaagtaagat ttctttgtaa ttaattttga tattattgca
     6901 gggagtaaga agattgaaga tgtagatatt attgcaaaaa tcaaatcgtc agatagtcct
     6961 actgtttgtt tgaagaaaat aaaataaaga cagtatatgt agcttgcaat gattaatgtt
     7021 gttattagta tcctcttcct cataatcctt tacattgtac tgaaatatta atagtatctt
     7081 caatctcccc tacttcatct gggttgtaag atatagataa tgttgctgtc ttctggtcat
     7141 atatcagttc cccactttta gaagagacag agatatcaca tccgtcttgt gtatatgtgt
     7201 tatctaagaa tgtcccgtat tttgaaaatg agattttgga tatataagaa tcatatggtg
     7261 tatggttttg tatttttaca gatcttttga tttctagagt atcttttcct cttgtaacga
     7321 tatctgtctt tgtaatagta tcgttttctg ttttggaggt gtatgtagga tatatatatc
     7381 ctatgttgta tccagattta gtatattcaa gtaaatcgtt ttcgaatacg tatgttggtt
     7441 tattgtcttt gctaagaaag tcttgttggt tatttatctc aaaccatatc ttatttggta
     7501 ttgttacaac tagtgagtta tttatatatt cgtatatgta aggtgttcta aatattcttg
     7561 ttccgtaaag tagagatata ccgaaggaga gtaaagcaaa gattagaaag aatattttaa
     7621 atagttttct catatttaaa tatatgggat atgtgtataa aagaaaaggg cacccaatat
     7681 tatttttttt gacagttaag tcaatagaat aatcctctgt taagaggggg gtgcccaatt
     7741 catatggagg tggttgtggc tattattgga caaatgtcca gctggtattt tctttcgtgg
     7801 cgatgtattg taattctttt gtttcctgac cagaataggc tttgtcgtag atgttaatct
     7861 gcgaaccttg atggccaatt tgaattacat ctcctggatg gagaatttga tagaaacaaa
     7921 tggcttcctt gtcacaccaa gtgaacagga cagaaccaac atcgcgagtt gtaccagatg
     7981 tatcaaaaac ttgattaggt actacccaat cctttcctct tgatccaaaa tagccaatga
     8041 aattggcgtt ggatccggct gatctgggat ctgggctttc taaggtgcca ttgtttggta
     8101 aaactggatt tgcaacgatc ggtgccgacc ctggggtttg agctgatact gcaggtttgt
     8161 tgagcaacat tattgcgatc aatgccagca gtgccaggag cccaaggatg ccaagaactt
     8221 tccagaagag attagaatct cccttggtct cgatcttgtc ttcagcaaca ggcgcagtag
     8281 cttttggagc aacaggcgca tttgccttgg cggattcgtt catctcaatg ttaatcgaga
     8341 ttttcttgat gtctttctct gtgattgaca tttctttctc ctttttcctt tatgagtgaa
     8401 tttttagttt cagtataagg tcgccacaac ctcaccttat ttattacata tttctatata
     8461 agaaataagg taaagttgta gctaaaagag aaaattattt gccataacct tccatatgat
     8521 acttgtataa atatggatta tattaatcac ataatacaag ttattttaaa gaaccttgtt
     8581 tctattaaaa gaaacattct agagattaaa ataatcgcta actatgaatc ttctaatgat
     8641 tggaaatgtt tttaaggagc aagtttttaa ttgtattaat ataatttatt aaattaattc
     8701 aatactgtag ggcaatgtat aaacagtgcc ctactctatt gaccaaaagc tactgcgctt
     8761 ttgttttact ctttaaatat ctaccctcta acatcaaacc tattcctaaa ccgaaaacga
     8821 ttaatgctat accagcaaat atactcttgt tatctaggat aaaagaggta tctacaggta
     8881 tatgtccata accatcagct aagatagagg atgtgttaag aagtataaat cctaaagaag
     8941 ttgccaatac agaaaatgtg tttttcataa ttgacattaa taaatttatt aaagaactaa
     9001 gagaattata ctataagcca ctataaatat caatatatgg gtatgaggag gctgtgtatc
     9061 ttaaactggt tctccgttga tagagacaag atttgcgaat ctaaatatac ggattgggga
     9121 gttataatac ttacaagtat ataggataag atctgcatca atatccttta tgtacttgtt
     9181 ctcatcaagt ttaaagattt ggtatatata ctctctttga ttccatatta taatcacctt
     9241 atcccctatc tttgtatttg ggaggttgaa gaaagatatc ttattcctgt attcagttgt
     9301 ccatgaagag tacccaaacc tgtgcgaagc tataattatg gatttagttg tttctccaag
     9361 atagttttgt tctggatttg catagtcgta aactatccat ggccccttat ctaatgcttt
     9421 gagagggtct tttccagtta taatttttgc ttttacacca atctttggaa ttattaatgt
     9481 atttgtctct gtaagagagg tatctttttc aggcagagtt atctgtggag gttctggagg
     9541 ttgttctgta tcgtcttcct tgttctctgt aacaggaatt tgcagagttt ctgcttctac
     9601 ttctggtgaa ctaatattta gtttgttgaa tatatatggt agctgaggat acgcaaggag
     9661 agagagagat gcaaaggtga gaatagcacc gatgataata tatatattcc ctgtagtatt
     9721 gattattttt tctcttttca tcactattaa tttttatttc ttaagaattc ttctaatatc
     9781 ataccacggt tcttcatctc tagagcattc gttttcccta tatatctata atgccaactt
     9841 tcataactaa atccagttac atgttcatat ccagaaggga aactaagaac aaaaccatac
     9901 ttgtgggcat tcttctgtaa ccaagcactt gcctttgtag tactaaacct tgtccctatg
     9961 ttgtcactaa cctctttact actaaaatct atagcagttc caagctgatg ttggctatgc
    10021 cctgctcttg cagatattct gtctgctgca tctacattac ctccattgta ctgtacccaa
    10081 tagttgtaag tacttacttg tgtagcgtat gatctgtatc cagaaactat gcttagatct
    10141 attccatccg ctttggcagc atttactaaa tctcgtaagt cgttaattag tatatttcta
    10201 acataaaaat tatctccatt tctaattcca gaattacttg cttttactaa gtcgtttgga
    10261 gcatatgtag atggtagttt gtactccttg tttacatgta ctaaaagatc attaccattt
    10321 ctttttacag ttttaatatt actaggatat gtccaccaag aaactgaagg tgtgtagtta
    10381 gagtatgtct tggggattat actttcctta atatcattta cctctatggt attggatgtc
    10441 ttaacattgt tcttttctac actctgtgta tcttgaaaat ctgtggtaac tacattaaag
    10501 gaaagaacta atgttgagag atatatccat atatttgaga agaattctgt cataacacaa
    10561 tattttagca caaaaaggtt agcgtgtttc aattgtgagt gggaattaag gggtattaac
    10621 tcccactcat ttgttatctg taccaaacat atgtttggta acttttctct atgttgattc
    10681 tttccccgct ttggcagatt tctttttttc cacctaagtt ctggaagaaa atatgctttc
    10741 caggagtcgg atagcaaata tattctcctt ttgggaaaaa agttggaaaa gaagttaaca
    10801 ttatcgtctc ttccgttccg aaaaggacaa cctttgggag atcagattgc atggattggc
    10861 ccactgccag aaatagcaac aatattaagg acaagacgaa gagtaacgac aaaaccttcc
    10921 ctccgaaatt atttctcacc attttctcct cctttaaata gagaaatatt gaaacacgct
    10981 atggaagtac actgattaaa tctttgtgta ctatttgaaa tgaacacttg attatactac
    11041 tatgggagtt atttttacta caagaacaag ctatgtcttt ctaggtcaag aaatataaat
    11101 tgagagattg taatatttaa atactaaatt atttctcctc acatatgtta accaaagaag
    11161 atttaaaggc catagagaaa atactttctc caaggtttga tacgttggac aagaggatgg
    11221 acaaactgga agaaaggatg gacaaactgg agaaaaagat ggtggagcta gaggagaagt
    11281 taaacttcct tgaggagagg ctagagaaaa catatgaaga attggataga agaatatgca
    11341 gtattgtaga tacggttggt gagattaatg gtactttaca aggattagct ttactggatg
    11401 caaatagcag aggagatata aaaagacagg agggtaatat ccaggaggtt tacaagatga
    11461 atattgaggt taatgataga ttagatgcta ttgaaaaaca tctaaataca aaatatgacc
    11521 cagtaagtag aagccttatg gataaatcta aaatagagta tgagcgaaag aagcttaaag
    11581 gatagctact gtagctacac tatcatcttc attcatgttc atcagtttta caccggatgt
    11641 ctgtcttcca agtgttggaa ctgctttaag ttcagatcta ataacttttg ctttttgaga
    11701 aataactact atctcttctt cacccttctc aattattctt gctgaagcta ttcttcctgt
    11761 tttgggagtt actctaaatg taaagactcc tgttccacct ctcttctgca gaggatactc
    11821 ttttaatgtt gtcatcttcc catacccatt gtctgagaga gttagcaatt tttcattgtc
    11881 atcttttatt attcccatcc ctactacata gtctgtagca tttttaaatt tgatagctgt
    11941 tactcccttt gtacttctac ccataggtct aacgtcagat tctttgaatc taacagattt
    12001 accatctgca gtaacaatca cgacattgtt gtttcctgtt gttggtttta cccatgtaag
    12061 ctgatctcca tcttcaagtt tgatagcggt taatccgttt gatctaatct ttttgtactc
    12121 ttcaagagct gttttcttta tgacaccctt ttcggttgcc attaagaagt atgagtaatt
    12181 gatactttct ccaccttcag tcttctccgt tattgttccg tttggatttc ttgttaagac
    12241 agaattaatt gtctcattct gttctagttg tacaaggtta actaaaggaa gaccttttgc
    12301 tgttcttccg tactctggaa tttcaaatat tctaatgttg aaaactctac ctctgtttgt
    12361 aaacagaaga agttcatcat gggtattaca aaggaatgca tgttcaatga agtcaccttc
    12421 tttagtttcg ccacctgaaa cacctttacc acctcttctt tgagatttgt aagtatctgg
    12481 agttaatctc ttgatgtaac cagaatgtgt tattgtaacc aacatctctt catttgcaac
    12541 catatcttct tctgagattt catttacctg tcctttaata acttttgttt ttctatcatc
    12601 accgtatttt tcttttatct catctaaatc tttggaaatg atttctaata tcctgtcatg
    12661 gttttctagc actgcgttgt attctttaat attgtcttgg agctctttat actcattttg
    12721 taatttcatc ctctcaagag cagccaatct tctaagttgc atatccagga ttgcttgtgc
    12781 ttgaacctca gtgaatttga aattatccat aaggttggtt tttgcagttt cttgggtttt
    12841 ggatccacgg atagttgcaa ttacctcgtc taacaaatct aatgctttaa gcaatccttc
    12901 aagtatgtgt gctctaaatc ttgcttctgc taagtcaaat tcatatcttc taatggtaac
    12961 tgtcaatcta aaggaaagga ataactccaa gattcttttt agtggtaatg tttttggctc
    13021 ttgctctacc aatgcaatca tgtttgcatt aaagaccttt tgcatttcag tatatttgta
    13081 gagtttgtta agaactgctt ttggttgtgc atctcttttg aggactacaa ctactctcat
    13141 tccttctcta tttgattcat ctctgatgtc tgcgatacca tctatttttt tctctttaac
    13201 aagatctgca aatttctcaa tcatgtgggc tttgttaacc tgaaatggta tctctgtgat
    13261 aatgatattg aatcttcctt gtttaccctc tacgattgaa gcttttgctc tttgaagaat
    13321 tcttcctctt ccagtagcgt atgcatttag gatttctttc tgatcatata tgattcctcc
    13381 tgttgggaaa tctggtccag ggattatttc tattaaatct agtggtgtaa tgtcagattt
    13441 aaattcagga tataaaatgg acttttcttc aattattttc tttcttaaat cttcaatatt
    13501 tttaacaaga tttggatctg caagatctac ataattctcg agataatctt caggtttaga
    13561 atttagagtt tgtggaataa tttcctttgc ttcttttgct tttcttaatt cattgtatat
    13621 accctttcct tcccatttgt ttcctctttt aatcatgtcc tgaagtgcag ttattagctc
    13681 tcgcaagtta tgtggtggca tctttgttgc catacctact gcaataccat ctgttccgtt
    13741 ggctagtaca tttggaaata gtgctggaag cacggttggc tcaagtcttg ttccatcgta
    13801 gttttgtgta aatgttactg tgcctttgtt taaatctcgt aaaagctcaa tagtgatctt
    13861 tcccattctt gcctctgtgt atctcattgc tgctgctcca tctccatcga tagaaccgaa
    13921 gtttccctgt ccatctacga gtacatatct agtagaaaaa tcttgtgcta gttttacaag
    13981 ggttggatat actacggact caccgtgagg gtggtagtta cctgaggtgt ctcctgcaat
    14041 ctttgcacat tttctgtaat gtgctgtagg agttagagct aagtcattca ttgctacaag
    14101 aattcttcgc tgagaaggtt taaggccatc ttttacttct ggtagtgctc tggaaacgat
    14161 aacggacata gagtaatcta ggtagttact tttcatttca tctacgatag atctttgtac
    14221 aacctgtcca ggtttaaagg aaagattgaa atctatctca ttggtttctt ctgttttatt
    14281 ttctatttcg tctggcatgg ttagtggtta ttaaatagcg ttatcttgta aagcttcctt
    14341 tacgttttgc attaccaata ttggatcttg tacatattcg gataaagcat cgtacgtctc
    14401 ttgagtgatt ttttccaaac cctcttcatc ccttgtcgac atatctatga aaaagattct
    14461 tttattttct ttatctaaga cattggctct aagtaatttc tcagaattat tatcttcaag
    14521 acagattccc tggttttctt taagctcttc tttaaaaatt tcttttaaat cttcattggg
    14581 atcctccata atagttgcaa atattgctga atggtatttg gttaattctg tagctacttt
    14641 catgtttaat aattgtttaa attcttcgga ctctggtgta ttcatatcac taacttcttt
    14701 tattcctgaa aatatattac tcattttaaa acatataaaa tttaaatatc taaggtagcc
    14761 ttacgtgcat acatctgtat aaatcttctt cttggaggaa cctcgttgcc cattaacatt
    14821 tcaaatgtct tttctacttc ctctgcatct tgtatctcta ctctcttaag tactctggtt
    14881 tctggattca tagtggtctc ccataactgt tctggattca tttctcccag tcctttgaat
    14941 ctattctcta caggaacttt accttccttc ttagcttctt taacaaaagc ttctttttct
    15001 gcatcattta agaagtaata tttctttttt ccaatttcta ctttgtgtag tggtggttga
    15061 gcaacatata tgtatccctg ctctatcaaa ggacggaaga atctaaagag taatgttaaa
    15121 accaatgtag tgatatgtaa tccatcgaca tcagcatcac tcatgatgac tactttgtgg
    15181 tatctaagtt tcgcaacatc cagtgtatcc cctattccaa caccaagtgc tgtggttaca
    15241 tcgttaaatt cattattaga aagtactcta tcaactctat atttttgtgt attcataatc
    15301 ttacctctaa gtggaagaat tgcttgtgta tgtctgtctc tcccctgctt tgcactgcca
    15361 cctgcagaat ctccctctac gatgaataac tcactctcgg aaggatcttt acttgaacaa
    15421 tctgctagtt taccaggaag gcttgtactc tcaagcactc cttttctaat gacagcatct
    15481 ctcgctgctt ttgcagctgc tttagctttg tttgctaata ctgccttccc aattatattc
    15541 ttaccttcat ttggatgctc ttcaagatat accaagagtt cgtctcttac taattttcct
    15601 acaatacttt taacctctgg gttatttaac ttgatttttg tttgtccttc aaattgcgga
    15661 ttaggcactt tcactgaaat aactgcagtt aatccctctc ttacatcgtc tccgtttagt
    15721 gttccattta gacccttaag taattctgct ttttgagcat atgtattaaa tgcacttgta
    15781 agagcagttc taaatcctgc taaatgcgta ccaccttcag gattgatgat gttgtttgtg
    15841 tagcatctaa catcctcttc cattccatct gtgtattgca aagatacttc aacgagtacg
    15901 ccggcatcct ccttgttcat gtagaatgga tcttctccta tggatttctc atcaatattt
    15961 agaaattgta cgtaagattt aattccatct tcaaagtaca aatcgtatct tgtatgtttt
    16021 tcttctcttt catctatgac ggaaattctt aatcctgctg ttaaatatgc gtgttgtcta
    16081 caacttctta caattgtatt aaattcaaat tctgtttctt gaaaaattgt tggatctggt
    16141 aaaaatgtat gctctgttcc tgtttcgttt gaatctccaa ttcttttaac tggggctttt
    16201 ggaacacctc tttcatatac ctgctgaaaa attccaccat ctcttctaac agttgttgtc
    16261 atctgtgctg aaagagcatt tgtacatttc attccaaccc cgtgaagtcc tcctgaaact
    16321 ttgtatgctc catcgtcgaa tttacctcca gcatgaagat tggtcataac tgtttctaag
    16381 gtggagactt ttgtctctgg atgaatggcc acaggaatac ctctaccgtt atctctaact
    16441 gtgagtgaat gatctttgtt aaaaattacc cagatattac tacagtatcc agcaatcgct
    16501 tcgtctactg agttgtctag aatctctgta aatatatgat gcagaccatt cttatctgta
    16561 gatccgatgt acattgcagg cctttttctt acagcctcca gtccttttag tacctgaata
    16621 tttgatgctc catagtcact cttatttggc attatattgt atgaatttgt aacttaattc
    16681 ccaagcacaa gtatcctata ttttactctt ttttggcaaa aatgttaagt atttagagat
    16741 acgattagtg cctctaatac aagctttttg ttgcaatttg agtctatttt tttcttagaa
    16801 tcatttaaca aatctagcgc attttttaat ctctttgggc tctcaatctt accatttttt
    16861 atttcaaatt caagtttctt tttaaatata ttttcaatgt tctcaatgaa cagaagagaa
    16921 tcctctttac tttttgagat cttctctaca aggaggaact gttctactag atcaagctca
    16981 agtatatctg aagtaacctc ttcttttttc tctacatctc cttttgcata tattaattta
    17041 cctctagaga ttatggtttg aagaagattt ctctcattgt ttacacagag tatgtatatc
    17101 gtgttgtcac tactctcttc caaggttttg agaaaagaat tctgtgcttg tgtagtcaat
    17161 ttatgactat cgagaataat tgctaattgt gaagcattgt tgaaaggttg aaaaatcatc
    17221 tcgttttgaa gtgcttttat ctcatcaatt ccaatgctat tctgttgtct accatctaca
    17281 atatgtatat ctggtacacc gaaaagctct tctaattcaa caaaattcgt cttcaaaaat
    17341 ctgttaacca aaagtacaag attgtcctcg agtatttttt ggttattgtt aataagaaga
    17401 tatgtcatat ctgattctat catatatata aatattgact gtagtaa
//
LOCUS       DAOQWP010000016        17127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_3743_length_17127_cov_7.657334, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000016 DAOQWP010000000
VERSION     DAOQWP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 17127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17127
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3743_length_17127_cov_7.657334"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PKI16_02600"
     CDS             <1..939
                     /locus_tag="PKI16_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNR53341.1"
                     /translation="GDIDFLESVMQSTHMALVRATLYQQLESFNKTLQEKVNEQTKEL
                     QIKVVELEEARKKERDMIDIMGHELRTPATVVKLNVELLQKYVNSNPEEFKRHLGRIK
                     EAIDTEIKLINTLLSSAKLEGNKIDINSEQVDILQEIEMCIHGHEREARAKRLKIVNK
                     TKKRTPTIYADKARVIEILNNLLDNAIKYTKKGSVTVKTEYDRQNVTIYVEDTGQGIP
                     KGELSKLGKKFYRVDNYLTGKDLDPVDIVRPGGTGLGLYVTFNLIKLMKGKILVESKL
                     GKGSKFIFTLPIYKGQDKKRPKKVSLNMFERMGFKS"
     gene            complement(904..2190)
                     /gene="eno"
                     /locus_tag="PKI16_02605"
     CDS             complement(904..2190)
                     /gene="eno"
                     /locus_tag="PKI16_02605"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNR53342.1"
                     /translation="MKIKNIQAREILDSNGKPTISTEIELLGGDKGVGDVPSGASTGS
                     TEVLELRDKDGLGVTDAVNIINTKIKDSIIEKEFKSQEEFDNYLIELDGTQQKSKLGG
                     NSILSCSMAFCRASTNSLGLELYEYISMIYSKEDYNPKNFTMPSPLVLVMEGGKHGDW
                     ATDIQEFMIVPNVERFKTFEDGFRQSIKVFQGIRDILKQKRYSTNVGFEGAFAPVEIK
                     DNKEALDIILQGIQESGYTPTEDFSIAIDVAASEIYDKTKQKYILKKEKKEYDTQRWI
                     SFQKELYSNYPLISIEDPLIENDWSGWNTITKALGEKYQIVGDDLLTTNTERIQQGIE
                     NKAMNSVLIKLNQIGTVTETLNAIKLSVDNGMTAIISHRSGETNDSFIADLVVGTPAT
                     QCKFGGPTRGERLVKYNRLLEIEKKLTLESHSFEHI"
     gene            2314..3126
                     /locus_tag="PKI16_02610"
     CDS             2314..3126
                     /locus_tag="PKI16_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53343.1"
                     /translation="MESQVENSMENSSSADSEPKREISQPRKSGFLWIMIFLIICLAG
                     GLTYYVLKDNVVDLSSSIPSTKETGTTDNKQCPECKIDPTNSGWSLFTVPGYNLSVEI
                     PTYTLTQKLESENIPSVWKVWYNNTALVSYLDDYIGTMNVHFYPTRIPEGMRCGQGCV
                     GEHTIYVNIHTNTNTQNLASAMSSYRTKWEKMYVEGSGGDIKSTNLKGETVSKWNTDV
                     WSFRYELPGGTWKGYLVVKDGYIYDVEYYLSDTPKASYDIALKVLDSLKFEQ"
     gene            3177..3263
                     /locus_tag="PKI16_02615"
     tRNA            3177..3263
                     /locus_tag="PKI16_02615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3211..3213,aa:Leu,seq:gag)
     gene            3274..3350
                     /locus_tag="PKI16_02620"
     tRNA            3274..3350
                     /locus_tag="PKI16_02620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3308..3310,aa:Val,seq:tac)
     gene            4231..4422
                     /locus_tag="PKI16_02625"
     CDS             4231..4422
                     /locus_tag="PKI16_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53344.1"
                     /translation="MNLNKVFVEIGGSIILGILGGIVTKSLLVGIGLPIVLVIFFVMM
                     YAVKPPTSDSGDDKDKTSQ"
     gene            4426..4499
                     /locus_tag="PKI16_02630"
     tRNA            4426..4499
                     /locus_tag="PKI16_02630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4460..4462,aa:Val,seq:cac)
     gene            complement(4501..5715)
                     /locus_tag="PKI16_02635"
     CDS             complement(4501..5715)
                     /locus_tag="PKI16_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53345.1"
                     /translation="MVKNILTSNSLFVRNLLFTSVFGGLIGLLNYLFNIFVARFTTME
                     IFGIYSATLGIIYLIQIPALSIQALITKAVAKNKLFNLNSYKWNILLQFTVLGVVFGG
                     IFLLFKDSISSIATIPSDLIIYLAITFFLAFVSPISKGLLLGKERIITVNLLLLFETI
                     LRFVVGYFAIKTSAPLPLLILSCAVPSVITTLATLPLINLKGNGEKKSVPIDFKELFL
                     ATVSFLLLTIPYTLDLILVNPEFRAEYSGVSLLGKLVYFAAITIASVMFARISNLTKK
                     AEITKSLLVALSLSVIIGLGTTAVLVIFKDSIISLSIGEKYLSVAPYLWIFGLCMTGY
                     ALVYMIANYFITQRYYIYILVLLVCSLLQVYLFSVRNESLEMVIVNQVIVYLVLTLGT
                     FGMLTLNLRKKE"
     gene            5779..6903
                     /gene="rmuC"
                     /locus_tag="PKI16_02640"
     CDS             5779..6903
                     /gene="rmuC"
                     /locus_tag="PKI16_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNR53346.1"
                     /translation="MLYVILALVGGILLGIGIFYFVNKGKGNSNEELSETIEEKLSEL
                     VPKALMEANEYLVKTARESLSSETKQNRIDLDNKREEIGRLIKNLEEYVKVSEKDRID
                     SFSSLKTSLEESRRVTEQLSVNTESLKKVLSNNQLRGQFGEQVADDLLKMAGFVRGND
                     YEFNKEQAGSETRPDFTIFLPDRTRINVDSKFPYNNLQRMSETDDKEAKEKYTTQFVR
                     DVKEKINQVCSRDYINPEDKTVDFVILFIPNEMIFSYIYEKMPEVWQEAMAKKVVLAG
                     PFSFTAILRMIKQAYENFRVQGNIHNIIGNIKAFEKEFENFSDSFYKIGERIGSLQKQ
                     YDTVSTTRFNQLIRRVDKVKLEGATPEEEQKILDFVKDVE"
     gene            complement(6887..7984)
                     /locus_tag="PKI16_02645"
     CDS             complement(6887..7984)
                     /locus_tag="PKI16_02645"
                     /inference="COORDINATES: protein motif:HMM:NF020477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HNR53347.1"
                     /translation="MNTTEVPLFEKLGFDSERYIELQKRDILERLSKVNNGKLYLEVG
                     GKLLEDPHAARVLPGFQLDIKTKILQRLDMPFDIVYCLIYGDILKNRQLNNQQEEYID
                     SAIKTIQKMEKLFGVKPYVVINNITDEKDLRFVQGKEKLETICNHIYLRYHIDGYPSD
                     TQKILSSDGYGKDEEIPVKNKLIIVTGAASNSGKMSTCFGMIYFDHLRGLNSGYAKYE
                     TFPVWNLPLEHPVNLAYEAATADIGDRNIIDEYHLKEYNEVSVNYNRDIDAFVILKKL
                     ASDLLPPENMLNNYKSPTDMGINHVKESITSDEIVCITSLQEIQRRKSWYQQVIDHGL
                     GENSWVSACQELENKALEYIQSKGYNPTLHL"
     gene            complement(8049..8627)
                     /locus_tag="PKI16_02650"
     CDS             complement(8049..8627)
                     /locus_tag="PKI16_02650"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNR53348.1"
                     /translation="MRRFISGAILSLLLLLPFAGITTPILAQTADDYVTTSLEEYDWN
                     TNYDWKSLVKDYDYSELADTTTGVGIIAALFGGVMLFVSIICSLGMYIYMALTLMKTA
                     QKLKAEDAWYAWIPILNVVLLFKLGEQNPWLILLSLIPGIGTLVVAILSIIATMKVCE
                     KRGYDKFFGLLMLVPVANLILWGMLAWGKKDV"
     gene            8717..9319
                     /locus_tag="PKI16_02655"
     CDS             8717..9319
                     /locus_tag="PKI16_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53349.1"
                     /translation="MDTADVKIIEGKLPVLLSAPHTYSHKRPSLSGSYKRGEVCTDII
                     VENICRNINCFGIMQSGETNYDPNFHKEKSNPYKQNIKEITKGIKFKHFVDIHGLSDG
                     GGYDIGIYYPTRFDRSMVFAQSISEGLNKGELKGASIYIFRFLDNDQETLGEYVASDL
                     RIPSVQIEIARYIREDEILRNAFIENLEKILKEKYNNIIG"
     gene            9403..10407
                     /gene="recA"
                     /locus_tag="PKI16_02660"
     CDS             9403..10407
                     /gene="recA"
                     /locus_tag="PKI16_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNR53350.1"
                     /translation="MAETVKKVVDPKGKAIEMAVSQIEKQFGKGSIMRLGSQEKVKVE
                     VIPTGALSLDIALGVGGIPKGRIIEIYGPESSGKTTLAIHILTEAQKKGEAVAFIDAE
                     HAFDPTYATNIGLDIDNLYISQPDFGEQALEILETLVRSAAFGVIVVDSVAALTPRAE
                     IEGEMGDSHMGLQARLMSQALRKLTAIAGKTGTTIIFLNQLRMKIGIMFGNPETTTGG
                     NALKFYASIRMDIRQKDKIVKDGQVIGHSRKVKIVKNKVAPPFKEATFNMIYPMGIDR
                     ESSILDAATDLAVVDKSGAWFKYGDEQLGQGREATIEVLKKDTKLREKIVKDVLSKVK
                     "
     gene            10411..11070
                     /locus_tag="PKI16_02665"
     CDS             10411..11070
                     /locus_tag="PKI16_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNR53351.1"
                     /translation="MKITALETQKRDNSRLNMYIDENFFCGISLNVVAKFGIYQGKEL
                     SDNELNEILNAELRERFFDRAVSFLVRSPKTEFQVRRYLRDLAFKKKGKWFKEISKED
                     ISAIEDFVIKKLLEYEYLNDEKYAQLFVEDRIKNKPRGKNILYGELISKGVSKDIVEK
                     VLGEMLGDEYAILVSTYKKRFKDESISFEDRKKIDFLRRKGFSWDLIEKFINDESSKQ
                     K"
     gene            11045..12727
                     /gene="recJ"
                     /locus_tag="PKI16_02670"
     CDS             11045..12727
                     /gene="recJ"
                     /locus_tag="PKI16_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNR53352.1"
                     /translation="MMSLVSKSNWIIPENKKENFVETILENRGIKNSKEHLSPSLDTI
                     PSYKKLYGATKAAKKILKYVKEDKKIVIHGDFDCDGICASALLWEFLFRDVSKVLGKK
                     VDVVPYIPSRIDQGYGLTESSIQDVKKLGCDLLITVDCGVRDKELINKYSKELDFVIT
                     DHHQPPEDISENLEYVLVHQMFPSKEYPYKEICGTSVVYLLIQALMYELKMEENREYG
                     LDLVALSTVTDMMPLLDVNRVFVKYGLEQISKGERLGLNALILRAGILPKDITSYHLG
                     YVVGPRINAAGRIGSPMEAVKLLVSNDEKKCNEISNELNNINFERQKLTTEIFDIAKE
                     DINLENKLLFVQGENWHEGIIGLVAGKLQEQFHRPVIVTTNNDGVIKGSARSIKGFNI
                     TKTLERFDKYLERYGGHELAAGFSAKVESMDGFVKKIVDFANKKITEKDLQRDIKIDL
                     LVDTQDIDNELINNLKKLEPFGYGNPKPIICLKDLIVVRKNIMGQEKNHMKLIVKGSG
                     VDLLTLVLFNCNEDTESINENDTIDVIGYPDLNVWNGNENIQFMVKEWRFSN"
     gene            12745..14046
                     /gene="aspS"
                     /locus_tag="PKI16_02675"
     CDS             12745..14046
                     /gene="aspS"
                     /locus_tag="PKI16_02675"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNR53353.1"
                     /translation="MKELNIRDVLKNPSEYNGKEITLKGWLFKFREQSKFGFLLLRDI
                     SGVVQCFVDDSEIIKQLSTTPSESVIYVTGIGKEEKQAPDGFELHLKSLEVISSPSVD
                     LPFQVYERKGDEEPNLALRLDNRWLDLRKPKNALIFKVWSTMEKYMREYWYEHNFIDI
                     KTSKLISIPSEGGAEVFKVNYFGQEMFLTQSPQFYKQMAMASGFEKVFEVGPIFRAEQ
                     SFTGRHATEFTGVDMEVSFVDKIEELLDLEEEWIVYWMTGLKGEMGEEIKEMFGKEIV
                     IPQRPFPRITFAQSMEILKKDYEDIGTEDEKTLGDYIKEKYNHEFVFITEWPWAKRPF
                     YHMKIEGKELSDSFDLLWNGLEITTGARREHRYDVVVKQAQEKGLDVKNFETFLQFFK
                     YGCPPHGGMGVGPTRLLMKLLDLDNIRDVTLLPRDPKRITP"
     gene            complement(14051..15361)
                     /locus_tag="PKI16_02680"
     CDS             complement(14051..15361)
                     /locus_tag="PKI16_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53354.1"
                     /translation="MTLTEAAFWTKRFGVVAGGALVVFLVVVLIITMVGRENNMPPAY
                     LTANFACTETKEEFLEHKLSIPTLKLADGSEMLFEIKTDSGKIDSLPSIINVYKFSNP
                     TQSLSSQADSKILAKKLGFNPDAVIRRGTESYVWVDRGTSRTLEISAKNLNFSLRTDA
                     QYIRTQAKNGTLPTEQEAKSKAANLLRSLGFASDDYASGIPTTTLIDVNPDGSFSQAA
                     SLSEAELVRVDFKRSKSMITIASNIVGADSMVATLTRRLGEPTQETPIINDERITVYT
                     FNTRVTTLNPNKSNISVYIGVQDKSSDELASVYQIDYTYWPIQETSCGTYELVSPQYA
                     IQQVQSGNGSLVYLNKVGGDSVVDYIPKKVTKFSVFYINLTYYESPTEQEYLQPVYVV
                     SGEVIFDDGSKGEFDYYYPAINYTSIQDKIVLPEPEVKETDSLF"
     gene            15419..17041
                     /gene="gltX"
                     /locus_tag="PKI16_02685"
     CDS             15419..17041
                     /gene="gltX"
                     /locus_tag="PKI16_02685"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNR53355.1"
                     /translation="MRTTEDNVVLSQLLFPDVVETPQSMFGKYPKRELPDGAMVLRFA
                     PSPTGFLHIGTVYMGMINTIIAKQTKGVSILRIEDTDKNREIENGVMTIVEGLRNFGI
                     DFDEGMIGDNQSKGEYGPYIQSKRLDIYKVFAKDLVSKGYAYPCFMTEQELENIRKEQ
                     EEKKLRTGCYGKWATWRDRSLEDVKAMLEKDEKFVIRLYSTGDIEKSFNMMDLIKGYV
                     TLRENDMDAVLLKSDGFPTYHFAHPIDDILMGITLVTRGDEWFSSVPLHVELFQKLGF
                     KQIPYAHFSPLMKTENGSKRKLSKRKDPEADVQYYISKGYPKEGVKEYLLNIANSNFY
                     DWKIQNPGKSSEEFALRLDKFNKAGSLFDIVKLDDMCKNYIASLSAEQVYNQALTWVQ
                     EYNKEVADILKENKDYCIQIFGIEREGPKVRKDIVKYSDIKEQLTIFFDTMFENIEYE
                     DISGIIESTKQKEILEKYLKVFEIPNSVETWFEGVKKLAEEMGVKTGEIAMVLRVAIT
                     KKTKSPDLYQVISILGEDKVRERLEKYLGKVI"
     gene            17053..17123
                     /locus_tag="PKI16_02690"
     tRNA            17053..17123
                     /locus_tag="PKI16_02690"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17085..17087,aa:Gln,seq:ctg)
BASE COUNT         6021 a         2636 c         3221 g         5249 t
ORIGIN      
        1 ggagatatag attttcttga aagtgttatg caaagtacac atatggcttt agttagagct
       61 actttgtacc aacagctgga atcatttaac aagacacttc aagagaaggt aaatgagcaa
      121 accaaagaat tacaaattaa ggttgtagag ttggaggaag cgaggaagaa agaaagagac
      181 atgatagata taatggggca tgagcttaga actccagcaa cagttgtaaa actcaatgtt
      241 gaattacttc agaaatatgt aaatagtaat ccagaagaat tcaaaagaca tcttggtagg
      301 ataaaggagg ccatagatac tgagattaag ttaatcaaca cacttttatc atctgctaaa
      361 ttggaaggaa ataaaataga tattaatagt gagcaagtag atatcctaca ggagatagaa
      421 atgtgtatac acggacatga acgagaggca agggctaaaa gactgaagat tgtaaataag
      481 acaaaaaaga gaactcctac aatatatgca gacaaagcga gggtaataga gatattaaat
      541 aatcttttag ataacgcaat taaatataca aagaagggta gtgtaactgt aaaaacagaa
      601 tatgacagac aaaatgtaac tatatatgta gaagacacag ggcaaggaat tccaaaggga
      661 gaattatcta aattaggtaa gaagttctac agagtagata attatcttac tggtaaggat
      721 ttagatcctg tagatattgt gagacctgga ggaacgggat tgggtttgta tgtcacattt
      781 aatttgataa agttgatgaa gggaaagatc ttagtagaga gtaaactagg caaaggtagt
      841 aaatttatat ttacattacc aatatataaa ggacaagaca agaaaagacc gaagaaagta
      901 tctttaaata tgttcgaacg aatgggattc aagagttagt tttttttcta tctcaagtaa
      961 tctgttgtac ttaactaatc tttctcctct tgttggtcct ccaaacttac actgtgttgc
     1021 tggagtaccc actactaaat ctgcgataaa agaatcattt gtttctccac ttctgtgcga
     1081 gattatagca gtcatgccat tatccacaga caattttatt gcatttaaag tctctgttac
     1141 agttccaatt tggtttaatt tgataagcac actattcata gctttattct caattccttg
     1201 ctgtatcctt tctgtatttg ttgtaagtag gtcatctcca actatctggt atttctctcc
     1261 taatgctttt gttatagtat tccatccact ccaatcgttc tcgattaggg gatcttctat
     1321 ggagattaat gggtaattac tgtaaagctc tttttggaag gatatccatc tttgtgtgtc
     1381 gtattctttc ttttcttttt ttaggatata tttctgtttt gttttgtcat atatctctga
     1441 agctgctaca tctattgcta tactaaagtc ttccgttgga gtatatccgc tctcctgtat
     1501 accttgcagt atgatatcca atgcttcttt gttatctttt atctcaactg gtgcgaatgc
     1561 tccttcaaat cctacatttg tagagtatct tttttgtttt agtatatccc ttataccttg
     1621 gaagacttta atactttgtc taaaaccatc ttcaaatgtt ttgaatctct caacatttgg
     1681 tactatcatg aactcttgga tgtctgttgc ccaatctcca tgtttaccac cttccattac
     1741 aagtacgagt ggactaggca tagtaaagtt tttgggattg tagtcctctt tggaatatat
     1801 catggagata tattcgtaca gctctagccc tagtgagttt gtacttgccc tacagaatgc
     1861 catagagcat gagaggatac tgtttccccc tagcttgctt ttctgttgtg taccatccag
     1921 ttctatgagg tagttgtcga attcctcttg acttttaaat tctttctcaa tgattgaatc
     1981 ttttatcttt gtattaatga tatttactgc atctgttacc cccagtccat ctttgtctcg
     2041 aagttctagt acctcagtac taccagtaga tgctccactt ggtacatctc caactccttt
     2101 atctccacca aggagttcaa tttctgtaga tatggttggt ttaccattgg agtctagtat
     2161 ttctcttgct tgtatgtttt taattttcat atgtcttttt ttgtgtatgt tagtgagtat
     2221 attgtgccgt atatttaata tttttacaat gtattgcatt tggtaaaaga gagtggtaat
     2281 gttttgatta agataattta agttagatat ataatggaat cacaggtaga aaacagtatg
     2341 gagaactcat cgtcagctga ttcagagcct aagcgggaaa tttctcaacc aagaaagagt
     2401 ggctttttgt ggataatgat atttttaata atttgtcttg ctggaggatt aacatactat
     2461 gtattgaaag ataatgttgt ggatctgtca tcaagtatac ctagtacaaa agaaacaggt
     2521 acaacagata ataagcaatg tcctgaatgt aagattgacc caacaaatag tggatggagt
     2581 ttgtttactg tcccaggata taatttaagt gtagaaattc ctacttatac actcacgcag
     2641 aaattagaat cagagaatat accatctgta tggaaggttt ggtataacaa tactgcacta
     2701 gtatcatatt tggatgatta tattggtact atgaatgtac atttttatcc tacaagaatt
     2761 cctgagggga tgagatgtgg acaaggatgt gttggtgagc ataccatcta tgtaaatatt
     2821 catacaaata caaacacaca aaatcttgct tccgcaatga gttcttacag aactaaatgg
     2881 gagaagatgt atgtagaggg ttctggtggg gatataaaaa gtacaaattt aaagggagag
     2941 acagtatcaa aatggaatac tgatgtttgg agttttagat atgagttacc aggtggaact
     3001 tggaagggat atctagttgt aaaagatggt tatatctatg atgtagagta ttatttaagt
     3061 gatactccta aagcatcgta cgatattgca ttgaaggtat tagattcgtt aaagtttgag
     3121 cagtaatacc aattgttttt agggtatttg tttggtaaaa tcttctctac atgtaagccg
     3181 aggtggtgga attggcagac acgctacctt gaggtggtag tgggatttat actcccgtgc
     3241 aggttcaact cctgtccccg gcatgttgta catggacgat tagctcagct ggctagagca
     3301 tctcgtttac accgagaggg tcataggttc gaatcctata tcgtccacca tcattttctt
     3361 cctatatcca tctattaaat acataaatat tcctaaagac aatataaatt ctactgattc
     3421 ataatcctgt ggcctaaaac cataattagt aaacaaatta ataaacaaag gaaagaaata
     3481 aaagaaaaat atatatgaag gtacaatctg ttctacaacc tttaaaaaag attgatttaa
     3541 agaaaatatc ttcttagata tagagaccat taaccaagag aaacatccaa gaaatccaag
     3601 aaggatatat acataaccaa tcttactatg tatcgagtca atattatgta tatttatctc
     3661 cccttgaaga ttcctttcca tgagagatac tggaacaagg tcggtattta ttccaccaaa
     3721 gattctctct ccccaactta tttcctctag tgcaacgaac agtaaagcaa tggagaagag
     3781 aaggtatatt acaaaaagaa ttttttcttc cttaaatcta aatgcaagta aaatactgat
     3841 gcaaacagaa agaatgagaa aaacaaattg tgtccattcc agtattccat cttcaatacc
     3901 tagcatctca tacagtggag tattcttaat taacaggtag aataaaatac ccccaattag
     3961 tagggggaag aaaaaaacaa atcctttcca aatcttttct ttcatattac taaaccaata
     4021 ttaacaatta agtatatgca ttggcaagga aaactgttgc gcttcttctt tcccttctat
     4081 acaatataca tatactgcca tggtaggaac aaagtcggat ataaaacata ttccaattcg
     4141 tagattatta gtattaatat ctctttatca ctaatagcag gcaaaaagat tgttgttaga
     4201 aagatttaaa ttttgtgcta tattattaat atgaatctta ataaagtgtt tgttgaaatt
     4261 ggaggtagta taattcttgg aatattagga ggtattgtta ctaaaagttt gttagttggt
     4321 atagggttac caatagtatt ggtaatattt tttgtaatga tgtatgcagt taaacctcca
     4381 actagtgata gtggagatga taaagataaa acttcccaat aatatggacg attagctcag
     4441 ctggttagag catcgcattc acattgcgaa ggtcattggt tcgataccaa tatcgtccag
     4501 ttattccttc tttcttaaat tcaatgttaa cataccaaaa gttccaagtg ttaatactag
     4561 atatacaata acctggttaa ctatcaccat ctccaaactc tcgtttctca cactaaataa
     4621 atatacctga agaagagaac atacaagtaa aacaagaatg taaatataat atctttgtgt
     4681 tatgaaatag ttcgcaatca tatacaccaa cgcataccct gtcatacata aaccaaatat
     4741 ccaaagatac ggtgctacag aaagatactt ctctccaatt gaaagagata tgatactgtc
     4801 cttaaagatt accaacacag cagtagtccc aagcccaata attacagaaa gagaaagagc
     4861 aaccagcagg gattttgtaa tctctgcttt ctttgttaaa ttactaatcc tagcaaacat
     4921 aacagaagct atagttatgg ctgcaaaata taccaattta cccaaaaggg aaacgccaga
     4981 atactctgct ctaaactctg gatttacaag aatcaaatct agtgtatagg ggattgtaag
     5041 aagaagaaaa cttacagtag ctaaaaacaa ctccttgaaa tctattggaa cacttttttt
     5101 ttctccatta cctttcaaat taatcaaagg cagtgtagcc aatgtagtta tcacagaagg
     5161 tacagcacaa gaaagaataa gcaaaggaag aggagcactt gttttaattg caaaataccc
     5221 aacaacaaac cttaaaattg tttcaaacag cagcaaaaga tttactgtaa taattctctc
     5281 cttacccaac agtaaacctt tagatatggg actaacaaat gcaagaaaga aagtaattgc
     5341 caagtaaatt attaaatcag agggaatagt tgcaatactg gagatgctat ctttaaaaag
     5401 aagaaatatt cctccaaaca caactccaag gacagtaaat tgaagaagga tattccactt
     5461 gtacgaatta agattgaaca acttgttttt ggctacagcc tttgttatta atgcttgtat
     5521 tgagagagca ggtatctgaa ttagatatat tattcccaaa gtcgcgctgt aaattccaaa
     5581 tatctccata gtagtaaatc tagctacgaa gatattgaaa agatagttaa gtaacccaat
     5641 taaaccacca aatacagaag taaaaagtaa attccttaca aaaagtgagt tactagttag
     5701 gatattcttt accatcttgg aactaagctt caagttatat ataattagaa tactctaaat
     5761 taaagaaatc ttccatttat gttgtatgta atacttgctc ttgttggtgg aattttactt
     5821 ggtattggaa tcttctactt tgtaaataag ggaaaaggca atagtaacga agaactctct
     5881 gagacaattg aagagaagct ctcagaatta gtacctaagg cactaatgga agcaaatgaa
     5941 tacctagtta aaactgctag agaatctctt agctctgaaa ctaaacaaaa cagaatagat
     6001 ttagacaaca agcgagaaga aattggaaga ctaattaaga atttggagga gtatgtaaag
     6061 gtaagtgaga aagacaggat agattcattt tcttctctta aaacttcact agaggaaagc
     6121 agaagagtta cagaacagct ttcagtaaat acagaaagtc ttaaaaaagt tctctctaac
     6181 aatcagctca gaggacagtt tggggaacaa gttgcagatg atctactaaa aatggctggt
     6241 tttgtgcggg gaaacgatta cgaatttaac aaagaacaag caggctctga aacaagacca
     6301 gactttacaa tatttcttcc agacagaacc agaattaatg tagattctaa atttccatac
     6361 aataatctac aaagaatgtc tgagacagat gacaaagaag ctaaagagaa atataccaca
     6421 cagtttgtaa gagatgttaa ggagaaaatt aatcaagtat gcagtagaga ctacattaat
     6481 ccagaggata aaacagtaga ttttgtaatt ctgtttattc caaatgaaat gatattttcc
     6541 tacatctacg aaaaaatgcc agaggtatgg caggaagcga tggctaaaaa ggttgtacta
     6601 gcagggccat ttagttttac agcaatacta agaatgatta aacaggccta cgagaatttc
     6661 agagtacagg gaaatataca taatattatt ggaaatataa aggcatttga aaaagaattt
     6721 gagaacttct ctgactcttt ctacaagata ggagagagga taggatcact acagaaacag
     6781 tatgatactg taagtacaac aagatttaac caattgatta ggagagtaga taaagttaaa
     6841 ttggaaggtg ctaccccaga ggaggaacag aagattttag attttgttaa agatgtagag
     6901 taggattata tcctttactt tgtatatact ctagagcttt attttcaagc tcttggcatg
     6961 cacttaccca actattttcc ccaagtccat gatctattac ttgttggtac cagctttttc
     7021 ttctttggat ttcttgaaga gaagttatac aaactatttc atcactagta atggattcct
     7081 tgacatggtt gattcccata tctgttggag acttgtaatt gtttaacatg ttctctggtg
     7141 gtagtaaatc tgaagcaagt tttttaagaa ttacaaacgc atctatgtct ctgttgtagt
     7201 ttactgaaac ctcattgtac tccttaagat ggtattcatc tatgatgttt ctgtccccga
     7261 tatctgctgt agcagcttcg taagcaagat taactggatg ttcaagtggc aaattccata
     7321 cagggaatgt ctcgtattta gcatatccag aatttaatcc tctgagatgg tcaaaatata
     7381 tcatgccaaa acatgtagac attttaccac tatttgaggc agcaccagtt acaatgataa
     7441 gcttattttt tactggtatt tcttcatcct tgccgtatcc atcactgctg agtatctttt
     7501 gtgtatcact gggatatcca tctatatgat atctaagata tatatgattg catattgttt
     7561 ctaatttttc tttcccttgt acaaaacgaa gatctttttc atctgtaata ttgtttatta
     7621 caacatacgg tttaacacca aaaagtttct ccattttctg aatagtctta attgctgaat
     7681 ctatatattc ctcttgctgg ttatttagct gtctgttttt aagaatatct ccatagatga
     7741 gacaatacac tatgtcaaaa ggcatatcta acctttgaag tatctttgtt ttaatgtcta
     7801 attgaaaacc aggaagtacc ctcgctgcat gcggatcctc gagaagtttt cctccaactt
     7861 cgagatacag tttgccgtta tttaccttac ttagtcgttc aagaatatct cttttttgca
     7921 attcaatata tctttcactg tcaaatccta acttttcaaa caaaggaacc tctgttgtat
     7981 tcatatattt gtgtaacaag aaattggata gcatactcta tactatccag tttatactag
     8041 attaattatt aaacgtcctt ctttccccaa gcaagcattc cccaaagaat gagatttgca
     8101 acaggaacta gcattaaaag gccaaaaaat ttatcataac ctctcttttc acatactttc
     8161 attgtagcaa taatggataa tatagctact actaaagtac caattcctgg aatcaaagat
     8221 aaaagaatta gccatggatt ctgttctcca agtttaaaaa gcaatacaac attaagtatt
     8281 ggaatccaag cataccacgc atcttcagct tttagtttct gtgctgtctt catcaatgtt
     8341 agagccatgt agatatacat acccaatgag catattattg aaacaaaaag catcacacct
     8401 ccaaaaagag ctgcgattat tccaactcca gtagttgtat ctgctagttc tgagtaatca
     8461 taatccttga ctagtgattt ccagtcgtaa ttcgtattcc aatcatattc ttcaagagat
     8521 gttgtgacat aatcgtctgc agtttgggct aatatgggag ttgtaattcc tgcaaaagga
     8581 agaagaagca atagtgaaag tattgcccca gaaatgaatc ttctcatcta aaaaaaggta
     8641 aaaaatttat atatatccat tctcacataa ctcagatatg tggtcaacag tgaaaatata
     8701 tgttacttta tatatcatgg atactgccga tgtaaaaata attgaaggga aattacccgt
     8761 acttctctct gcaccacaca cttactctca caaaagacct tctctttctg gttcttacaa
     8821 gcgtggagag gtatgtacag acattattgt tgaaaatata tgtaggaata ttaattgctt
     8881 tggaatcatg caatctggag agaccaatta tgaccccaat tttcacaaag agaaaagtaa
     8941 tccttacaaa cagaacataa aagagataac aaaaggaatt aagtttaaac actttgtaga
     9001 tattcatgga ttgagtgatg gtggtggtta cgatattggt atctactatc caactagatt
     9061 tgacagatcg atggtatttg ctcaaagcat ctctgaaggt ctaaacaaag gagagctgaa
     9121 aggtgcaagt atatatatat ttagattcct agataatgat caggaaacgc tgggagagta
     9181 tgtggcatca gaccttagaa ttccatcggt acagatagag attgcaagat atattcgtga
     9241 ggatgagata ctgagaaatg cattcataga gaatctagaa aaaattctga aggaaaaata
     9301 taataatatt atcggttaat taaatctttc ttttactgct ttgactgtat cctcttactt
     9361 tgttatcctg aagtatcact aaattcatag taattactac atatggcaga aacagtaaaa
     9421 aaggttgtag atccaaaagg taaggcaatt gaaatggcag tatcccagat agaaaaacag
     9481 tttggtaagg gttcaatcat gagacttgga agtcaagaaa aggtaaaggt ggaggtaata
     9541 ccaacaggtg cactctcttt ggatattgca cttggagttg gagggattcc aaagggaaga
     9601 attattgaga tatatgggcc agaatcttct ggtaaaacaa cattagcgat acatatcctc
     9661 actgaagcac agaagaaagg tgaagctgtt gctttcatag atgcagagca tgcgtttgat
     9721 cctacatatg ctacaaatat tggcttagat atagataacc tgtatatatc tcaaccagat
     9781 tttggagaac aggctctaga gatactagaa accttagtaa gatctgcagc ttttggagta
     9841 atagttgttg actctgtcgc tgcacttact ccaagggcag aaattgaggg agaaatgggg
     9901 gacagccata tgggtttaca ggcaagatta atgtctcaag cacttagaaa actcacagct
     9961 attgctggca aaactggtac tacaataatc ttcttaaacc agttaagaat gaagattggt
    10021 ataatgtttg gaaatccaga aacaacaaca ggtggaaatg cactgaaatt ttatgcttcc
    10081 atacgtatgg atattagaca gaaggataag attgtaaaag atggtcaagt aattggacat
    10141 tcaagaaaag taaaaattgt taagaataaa gtagctcctc catttaaaga ggctacattt
    10201 aatatgattt atcctatggg tatagaccgc gaaagtagta tcttagatgc tgcaaccgac
    10261 ctagctgtag tagataaatc tggtgcatgg tttaaatatg gtgatgaaca attaggacag
    10321 ggaagggaag ctacaattga agttttaaag aaagatacta aactaagaga gaagattgtt
    10381 aaagatgtcc tttccaaagt aaaatagggt atgaaaataa ctgcacttga gactcagaag
    10441 cgagacaact ctcgtttgaa tatgtatata gatgaaaact ttttctgtgg aatatcacta
    10501 aacgttgttg ctaaattcgg gatatatcaa ggtaaggaac tctcagataa tgaattaaac
    10561 gaaatactca acgcagaact aagagagaga ttttttgaca gggcagtatc atttcttgtt
    10621 agatctccta aaacagaatt tcaagtaaga agatatttaa gagatttagc atttaagaaa
    10681 aaaggtaagt ggtttaaaga gatatctaaa gaagatatct ctgcaattga agattttgta
    10741 attaaaaaac ttttggaata tgaataccta aatgatgaaa aatatgctca actgtttgta
    10801 gaagacagga taaaaaacaa accgagaggt aagaatatcc tgtatggtga actcatatcc
    10861 aaaggagtat ctaaagatat tgttgaaaaa gtactagggg aaatgctagg tgatgaatac
    10921 gctatacttg tatctacata caaaaaaaga tttaaagatg aaagtatttc ttttgaagat
    10981 agaaaaaaga tagattttct gcgaagaaag ggatttagtt gggatttaat tgaaaaattc
    11041 ataaatgatg agtctagtaa gcaaaagtaa ctggataata ccagagaaca aaaaagaaaa
    11101 ctttgtagaa acaattcttg aaaacagagg tattaaaaac agtaaagagc atctctctcc
    11161 atctcttgat acaatcccct catacaaaaa actctacggt gctactaaag ctgcaaagaa
    11221 gattctaaag tatgtaaagg aagataagaa gattgtaatt cacggagatt tcgattgcga
    11281 tggtatctgt gcttctgctc ttctttggga gtttcttttt agagatgttt caaaagtttt
    11341 aggcaagaaa gtagatgtag tcccatacat cccaagcagg attgatcaag gatatggtct
    11401 tactgaatct tccatacaag atgttaaaaa attaggatgt gatttactca taacagtaga
    11461 ttgtggagta agggataagg aattaatcaa taaatattcc aaagaactgg attttgtcat
    11521 aacagatcat catcaaccac cagaagatat ctccgaaaat cttgaatatg tccttgtgca
    11581 tcagatgttc ccaagtaaag agtatcccta caaggagatt tgtggtacat ctgtagtata
    11641 tcttctaatc caagccttga tgtatgaatt aaaaatggaa gaaaacagag agtatggatt
    11701 agaccttgta gcactctcta cagtcacaga tatgatgcca cttttagatg taaacagagt
    11761 atttgtaaaa tatggactgg agcaaatctc taaaggagaa agacttggtc taaatgcatt
    11821 aatcctgcgt gctggtattc taccaaaaga tattacctca tatcacttag ggtatgtagt
    11881 tggtccaagg ataaatgctg caggaagaat tggctcaccc atggaagcgg ttaagttact
    11941 tgtaagcaat gatgaaaaga aatgcaacga gatatccaat gagttaaaca atattaattt
    12001 tgaaagacag aaattaacga cagagatttt tgacatagct aaagaggata tcaatttaga
    12061 aaacaagcta ctctttgtac aaggagagaa ttggcatgaa ggaattattg gacttgttgc
    12121 gggtaaactt caggagcagt tccacagacc agttatagtt acgacaaaca acgatggtgt
    12181 aataaagggt tctgctagaa gtattaaggg tttcaatatt actaaaacgc tagaaagatt
    12241 tgataaatat ttagaacgat atggtggtca tgaattagct gcaggtttta gtgcaaaagt
    12301 agagagcatg gatgggtttg taaagaagat tgtagatttt gcaaacaaga aaattacaga
    12361 gaaagattta caaagagata ttaagataga tcttttggta gatacacagg atatagataa
    12421 tgaattaata aataatctta aaaagctaga gccatttgga tatgggaacc ctaaaccgat
    12481 tatatgtttg aaagatttaa ttgttgtaag aaaaaacata atggggcagg aaaaaaacca
    12541 catgaagtta attgtaaaag gaagtggtgt agatcttctg acacttgttc tgtttaattg
    12601 caatgaagat acagaaagta ttaatgaaaa tgatactata gatgtaatag ggtatcctga
    12661 tctaaatgta tggaatggta atgaaaatat acaatttatg gtaaaagaat ggagatttag
    12721 taattaatct gttagaattt ccctatgaaa gaattaaata taagagatgt tctaaaaaat
    12781 ccctctgagt acaatggaaa agaaattact cttaagggat ggttattcaa atttagggag
    12841 caatctaagt ttggtttttt acttttgaga gatatctctg gagtagtaca atgttttgta
    12901 gatgatagtg aaattattaa acaactctct accacaccct ctgaatcagt aatatatgta
    12961 acaggtatag gaaaagagga aaagcaagct cctgatggat ttgaattaca tcttaaaagt
    13021 cttgaagtaa tatcctctcc ttctgtagat cttccattcc aagtgtatga aagaaaggga
    13081 gatgaagaac caaatcttgc attaaggttg gataacagat ggttggattt aaggaaacca
    13141 aagaatgccc taatcttcaa ggtatggtca accatggaga aatatatgag agagtattgg
    13201 tatgagcaca atttcataga tattaaaaca tccaaattaa tttccattcc aagtgagggt
    13261 ggtgcagagg tgtttaaagt aaattacttt ggacaagaga tgtttttaac tcagagtcct
    13321 caattctaca agcagatggc catggcttct ggattcgaga aagtctttga agtaggtcca
    13381 atctttagag cagagcaatc gttcacaggt agacatgcta cagaatttac aggtgtagat
    13441 atggaggtat cttttgtaga taaaatcgaa gaacttctgg atttggaaga agaatggatt
    13501 gtgtattgga tgactgggct aaaaggagag atgggagaag agataaagga gatgttcgga
    13561 aaagagattg ttatacctca aaggccattt ccaaggatta catttgcaca atcaatggag
    13621 attctaaaga aagattatga agatattggt acagaagatg aaaaaacact tggtgactat
    13681 attaaagaga aatacaacca tgaatttgta ttcataacag agtggccatg ggcaaagcga
    13741 cctttctacc atatgaagat agaagggaaa gaactctccg atagttttga tcttctttgg
    13801 aatgggttag aaattactac tggtgcaaga agagagcaca gatatgatgt agttgtaaaa
    13861 caagcacagg aaaagggatt agatgttaag aattttgaaa cattcctgca attctttaaa
    13921 tatggatgtc ctccacatgg tggaatggga gtaggtccaa caagattact aatgaaatta
    13981 ctagatttgg acaatattag ggatgtaaca cttcttccaa gagatccgaa gagaataact
    14041 ccataagttt ttagaacaaa gaatctgttt cttttacctc tggttctggt agaactattt
    14101 tatcttggat agaagtatag ttaattgctg gatagtaata atcgaattct ccctttgagc
    14161 catcgtcaaa aatcacttct ccagatacta catatacagg ttgtaaatat tcttgttctg
    14221 taggactctc gtagtatgta agattaatgt aaaacacact aaactttgta actttcttag
    14281 gtatgtaatc aactacacta tctccaccga ccttatttaa atatactaga cttccatttc
    14341 cagattgaac ctgctgtatt gcatactgtg gagaaacaag ttcatatgtt ccgcaacttg
    14401 tctcttgaat tggccagtaa gtataatcaa tctggtaaac actagctaac tcatctgaag
    14461 atttatcttg tacgcctatg taaacagaaa tattactctt gtttggatta agagtagtaa
    14521 ccctagtatt gaaagtatat actgttattc tctcgtcatt aataattggt gtctcctgtg
    14581 taggttcacc caaccttctt gtaagtgtag cgaccataga gtcagcacct acaatatttg
    14641 atgcaattgt tatcattgat ttggatcttt tgaaatctac ccttaccaat tcagcctcag
    14701 acagagaagc tgcctgacta aatgaaccat ccggattaac atctataagt gtagtggtag
    14761 gaatcccaga tgcataatca tcagaagcaa acccaagcga tcttagtaaa tttgcagcct
    14821 tagattttgc ttcttgctct gtagggagag ttccattttt agcttgtgta cgaatatatt
    14881 gtgcatctgt tctaagacta aaattcaaat tctttgcaga tatctccaaa gttctacttg
    14941 tacccctatc tacccagaca tagctctctg tacctcttcg aattactgca tctggattaa
    15001 aaccaagctt tttggccaat atcttggaat ctgcctgtga acttaaggac tgtgtaggat
    15061 tactaaattt gtaaacattt ataatgctgg gtagtgaatc tatctttcca ctatctgtct
    15121 ttatctcaaa caacatctct gagccatctg ccaatttcaa agtagggatg gaaagcttgt
    15181 gctctagaaa ttcctcttta gtctctgtgc acgcaaagtt tgcagttaaa tatgctggag
    15241 gcatattatt ttctctacca accatggtta ttattaacac tacaacaagg aaaacgacta
    15301 aggctccccc agctacaaca ccaaatcttt ttgtccagaa tgctgcttct gttaatgtca
    15361 tgatataatc ttaacagtag ttatatattc aatcaatatt aatattacca attatactat
    15421 gagaacaaca gaggataatg tcgtactttc gcaattgctt ttccctgatg tagttgaaac
    15481 acctcaaagt atgtttggta aatatccaaa aagagaatta ccagatggtg ctatggtact
    15541 tagatttgct cctagcccta caggattttt acatataggt acggtatata tgggaatgat
    15601 taatacaata atagcaaaac aaacaaaagg tgtttcaatt ctaagaatag aggatacaga
    15661 taaaaataga gaaatagaga atggggtaat gactattgta gagggactta gaaattttgg
    15721 gattgatttt gatgaaggta tgattggtga taatcaatca aaaggtgaat atggaccata
    15781 cattcaaagt aaaagattgg atatctataa agtctttgca aaagatttag tttctaaagg
    15841 atatgcatac ccatgtttta tgacggaaca agagcttgag aatattagaa aagagcaaga
    15901 agaaaagaaa ttaagaacag gatgctatgg taagtgggca acttggagag acagaagcct
    15961 ggaagatgta aaagcaatgt tagaaaagga cgagaaattt gtaattagac tttactctac
    16021 aggagatatt gaaaaatctt ttaacatgat ggatttaatc aagggatatg ttacattgcg
    16081 agaaaacgat atggatgctg tacttttaaa gtcagacggg tttcctacat accattttgc
    16141 ccatccaata gatgatatcc ttatgggtat aactctagtt acaagaggag atgaatggtt
    16201 ctcatctgtt cctttacatg ttgaactttt tcaaaaactt ggttttaaac agattcccta
    16261 tgctcatttt tctcctttga tgaaaacaga aaatggtagt aagagaaaat taagcaaaag
    16321 gaaagatcca gaagcagatg ttcagtacta cattagtaaa ggatatccaa aagaaggtgt
    16381 taaggaatat ctattaaata ttgctaactc caatttctat gattggaaaa ttcaaaatcc
    16441 aggaaaatct tctgaagaat ttgctttaag gttggataaa tttaataaag caggatcgct
    16501 ttttgacatt gtcaaactag atgacatgtg taaaaactac atagcatctc tttcagcaga
    16561 gcaggtatat aaccaggctc taacttgggt acaggagtat aacaaagagg ttgcagatat
    16621 tctcaaagaa aacaaagatt actgtataca aatatttgga attgaaagag aagggccaaa
    16681 ggtaagaaag gatattgtta aatattctga tattaaagag cagttaacaa tattttttga
    16741 tactatgttt gagaatattg aatatgagga tatatctggg ataatagaga gtactaaaca
    16801 gaaagaaata ttggaaaaat atcttaaggt atttgaaatt ccaaatagcg ttgaaacatg
    16861 gtttgaggga gtaaagaagc tagctgaaga aatgggtgtg aagacaggag agatagctat
    16921 ggtacttaga gtagctataa ctaaaaaaac aaaatctcct gatttatacc aagtaatatc
    16981 tattttggga gaagacaaag tgagagaaag gttggagaaa tatctgggta aggtgatata
    17041 attatccatt agtgccggat cgtctaatgg taggacgacg gtctctggaa ccgttaattc
    17101 tagttcgaat ctaggtccgg caattta
//
LOCUS       DAOQWP010000017        14123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_4790_length_14123_cov_6.906241, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000017 DAOQWP010000000
VERSION     DAOQWP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 14123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14123
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_14123_cov_6.906241"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /gene="rplP"
                     /locus_tag="PKI16_02695"
     CDS             complement(<1..247)
                     /gene="rplP"
                     /locus_tag="PKI16_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNR53356.1"
                     /translation="MLEPRKRKYRKEFRGKMGGVASSNNEIVFGDYGLKAMENGWVNA
                     REIEAARRSITNFTNRKGKVWVKIFPHKPYTMKSTNSK"
     gene            complement(267..914)
                     /gene="rpsC"
                     /locus_tag="PKI16_02700"
     CDS             complement(267..914)
                     /gene="rpsC"
                     /locus_tag="PKI16_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNR53357.1"
                     /translation="MGRKVNPIAIRMGINKGWNATWYSEKKNFPKTFAQDIKIEAKIR
                     ETMRDAGIDRVEINRSANKVEIIVYVARPGVAIGRGGEGIDKLQKELKRLTRADIEVK
                     IREVKKADISASIIARGIADGIERRQPSKLLVQSAKEKAMMAGAKGLRIWVSGRINNA
                     SQARTVKVTAGSVPAQTLKADIDYAAETAKTIDAGLMGIKVWIYKGNKTEVATEE"
     gene            complement(916..1257)
                     /gene="rplV"
                     /locus_tag="PKI16_02705"
     CDS             complement(916..1257)
                     /gene="rplV"
                     /locus_tag="PKI16_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNR53358.1"
                     /translation="MEEKTVQVNVKDIAQSPQKLRLVADTVRGKQADKALDILMVLNK
                     KGCDIVKKALLSGIASARELYSVDKDELIVSKISVDESRTLKRVRFASRGRTSRITKR
                     RSHLNLELKVK"
     gene            complement(1277..1582)
                     /gene="rpsS"
                     /locus_tag="PKI16_02710"
     CDS             complement(1277..1582)
                     /gene="rpsS"
                     /locus_tag="PKI16_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNR53359.1"
                     /translation="MARSLKKGPYTDEKLMKKVTMARDSGKMTPIKTWARASTITPEM
                     VGMKFLVHNGKKHEEVLVIESMIGHRLGEFSPTRKFRGHAKKGKLSKVYGSSGRFEE"
     gene            complement(1600..2451)
                     /gene="rplB"
                     /locus_tag="PKI16_02715"
     CDS             complement(1600..2451)
                     /gene="rplB"
                     /locus_tag="PKI16_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNR53360.1"
                     /translation="MAVRNFNPTTSSRRGTKLGDRSGLEKAKGPRGLTVAKKQKSGRN
                     NMGKITVRHRGGGFKRRVRVVDFKRDKFGVPGKVEGFYFDPNRTAHLALLQYEDGERR
                     YIIAPKGIKVGDTLVSGEKADILPGNAMMVKHIPSGTIVHCVENIPGKGATFGRSAGG
                     AIIVQGPDTTGKYVQLKMPSGEIRLVYGECMATIGLVGNEDKVNVKLGKAGRKRNLGW
                     RPTVRGMAMHAVQHPHGGGEGKGVIGRHAKDIWGHRIGTRTRHNSMTEKFIIKTRRTK
                     NRPFAKK"
     gene            complement(2454..2747)
                     /locus_tag="PKI16_02720"
     CDS             complement(2454..2747)
                     /locus_tag="PKI16_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNR53361.1"
                     /translation="MESKNIQLQPVISEKSYNIANAQNKYTFLVNRGVNKIEMRKAVE
                     EKYKVHVEKVNSTVRAGKQFKNWKTNKSYRKSDAVKYTVTLKKGDKIDEFLAK"
     gene            complement(2737..3345)
                     /gene="rplD"
                     /locus_tag="PKI16_02725"
     CDS             complement(2737..3345)
                     /gene="rplD"
                     /locus_tag="PKI16_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNR53362.1"
                     /translation="MVEKKVTKTTEEKKISLNKKVWEVPYNGDLVSQVLGVYFSNERK
                     GTSNAKTRGDVSGGGIKPWRQKGTGRARSGSIRSPLWVGGGVTFVPNNKNWSKRINKG
                     MARKATCIMLSERLRNKELEFINIEATELNKIRKDIEGDKHNRIMVISDDKNVIAALR
                     NMEKIITIEGGKVNAKHLASAKKILVDNNVVKLLEERLTNGK"
     gene            complement(3347..3931)
                     /gene="rplC"
                     /locus_tag="PKI16_02730"
     CDS             complement(3347..3931)
                     /gene="rplC"
                     /locus_tag="PKI16_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNR53363.1"
                     /translation="MKALIGIKKGMTRVVKGDNIVPVTVLDVTGCILSNKEAMGFELG
                     LGEKKKSTKALAGKYKEAKRIPLFRKYFKGDLGEEVKIGDEIKAEMFTVGEKVTVTGT
                     SKGKGFAGVVKRWGFKGGQRTRGQSDRLRAPGSIGAGTDPGRVLKGKKMGGRMGQDTV
                     TIKDKEIIDIKENYILISGPVPGPKGQVVSIFTE"
     gene            complement(3948..4259)
                     /gene="rpsJ"
                     /locus_tag="PKI16_02735"
     CDS             complement(3948..4259)
                     /gene="rpsJ"
                     /locus_tag="PKI16_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006720268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNR53364.1"
                     /translation="MVNTTARIRVKLKGYDSVVVDKSAKRIIDTAISTGAKVAGPIPM
                     PTKRKKIAVNRSPFIYKSSIEHFEISTHKRVIDILDPTPKTIDALQHLEMPAGVGIAI
                     Q"
     gene            complement(4325..5509)
                     /gene="tuf"
                     /locus_tag="PKI16_02740"
     CDS             complement(4325..5509)
                     /gene="tuf"
                     /locus_tag="PKI16_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNR53365.1"
                     /translation="MADFNRTKPHMNIGTLGHIDHGKTTLVRGILNAHTTTFASDDEK
                     AKMIDKLDKDPSSRAKSITIAVAHVEFETENRHYALVDCPGHKDYIKNMITGAAQMDG
                     AILVISSNDGAMPQTKEHILLAKQVGVPKMLVFINKFGEADEEILELIKADVQDLLKK
                     YGYDENCPIIVGDALKAQNGEEEGLQAARDLMKAVDEYIPLPERDIEKPFLMPIEDVF
                     SIEGRGTVVTGRIERGIIKVGDAVDVLGMGKKPQKTAVTGIEMFNKTLKEGQAGDNVG
                     ILLRGLKREDVERGQILAIPGSVNTHSEFKAEVYVLSKEEGGRWTPFVTGYKPQFYIR
                     TADVTGEVILNEGTEMVAPGDNTEFTVKLISPVALEKGVSFAIREGGQTVGQGIVTEI
                     IK"
     gene            complement(5641..7740)
                     /gene="fusA"
                     /locus_tag="PKI16_02745"
     CDS             complement(5641..7740)
                     /gene="fusA"
                     /locus_tag="PKI16_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNR53366.1"
                     /translation="MDKTSYNVKMPLDMIRNIGIIAHIDAGKTTTTERLLFFTGQVHK
                     IGEVHEGAATTDFMVQEQERGITIMSAAVTCFWDYNQKRYRINIIDTPGHVDFTAEVE
                     RSLRVLDGAAVIFDGRKGVEPQSETVWRQANKYKVPRICFMNKINLIGGDFEKSFETI
                     KTRLSNKAVAVTLPLGKEQQLFGYIDLIKMRAYTYDGIDTPELTEIDIPEDMKEKAEK
                     YRAILLEVLVEQDDELMNKYFEGGEFTPEELYGALRKATLGLKMFPVLGGDSRSAMAK
                     TLLDYITICLPSPLDVPAVKGVHPKTGEEIERHPDENEPFSSLVFKIVNDPHIGNLSY
                     FRVYSGKIDAGTYVLNSTKNIKERVGRLVLMHADDREEVPSLRAGDIGAIVGLKDSIT
                     GDTLCEEAKPIILEKIDFAEPVVSEAIEPATKSDEEKMTEALVRLTKEDPTFKVTTDQ
                     DTSQTIIHGMGELHLEIIVDRLKREFNVEAKVGKPQVAYRETIKKSVPEAEGRYIKQS
                     GGKGQYGHCWIRLEPNGQGKGFEFVNAIKGGAIPKEFIPAIEKGIVEAMKSGVVAGYP
                     VVDVKVTVYDGSYHDVDSSEAAFKVAGSMAFKAGCKMGDPVLLEPVMKVEVETPDQYM
                     GDVTGSLSSKRGQIQGTESIGNGISKISAFVPLSELFGYTSELRSITSGRGSSNMEPS
                     HYAEVPRNVAEEISGKK"
     gene            complement(7816..9261)
                     /locus_tag="PKI16_02750"
     CDS             complement(7816..9261)
                     /locus_tag="PKI16_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNR53367.1"
                     /translation="MKEKLKKLAPWFVLAVILLGTGIWYLLSKRITQEQITSYQQIIS
                     EADSQYEAREYSTAINRYYDAADVIPSRVEAYEGIVDILLLKNRTSDAVEIVEKSAKN
                     ISNYDKSILYLKIGNKYYEDSLYDQALSTYLDGLGVGMTNPELELSIAKTYLKKGNLN
                     DANTYLSKSIYTEDTLYESTLLRAYILATTNIDDAKKVLGSIQPSDTWKAFYDEFSEI
                     LESLTEDSKFNATKLSRVYINSGYPVLAVNLLEPLSEDITEYLEGVYFLGRAYLDSGE
                     YDKAITQFDRALSLGGMEEDILWGQARAYLLKDDLENSFSSYDKAISYAGEDVSQELV
                     SEYMDLLLENNQTLRADGMVKKLMNYISSPYIRLYGVKVNKALNDSKKVDYYIGLLSK
                     MKLNDAEKKEYMYLNASNLLSKGKTDLAAQVLEELVKLDRFNPQYYFVSGQLEYNLGN
                     KDNAKELLKKAIEYDTNNLVTEEANQLLSQI"
     gene            9333..10256
                     /locus_tag="PKI16_02755"
     CDS             9333..10256
                     /locus_tag="PKI16_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNR53368.1"
                     /translation="MFKTRLIGIVLAIALMFVMSLIPTANWTDKEARYRSNTLIHMVY
                     GIDADVEKGRYTPKVVVVNYEGEIKKHISTASSPVFALLELGYPISTMKKVVSTSPMN
                     TLYNNSYIYVFSYRDTIDEVKLEIPYQRIVKGGTLCERLSQKVLQQEGVLGIMTQKVR
                     RVYERGELVMEEILEKDITREPRAEIYIIKGPDDSPKEVPQIGNNCDYWFAYVDTIKA
                     SEEEKRWLKWTMKYESGCNAENNRHPYYKGLFQWDPCLWYQQYPNDNIFDGRAQIKRT
                     LEKVRGGANPARMWPAVYKLYVDKYGELSWL"
     gene            complement(10253..12262)
                     /locus_tag="PKI16_02760"
     CDS             complement(10253..12262)
                     /locus_tag="PKI16_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNR53369.1"
                     /translation="MEQDIILGKLNPQQKKAVMCTEGPLLVFAGAGSGKTRVITNRIA
                     YLIAVKGVKPENILAVTFTKKASGEMLERVRKLLKDLNRDVTDLPMIGTFHSIGATLL
                     RRKAQEVGLLHNFSIYDSDDSENLIREIMLEMQIDIKQIKPKAIAHFIEAAKNDMVTP
                     AQFSNHFGGYIEDIAAQIYPVYQNQLKAQNSVDFGDLLFLTVKMLEENEEVRKYYQDK
                     YKYILIDEYQDTNNAQYRFAKLLSEKHQNICVVGDDDQGIYAWRGADIKNIQSFEKDF
                     KNVTVIKLEQNYRSTKNVIEAAVSVIQQNNQRIVKNLWTENNQGETITVYQARDQEEE
                     AEYVVENIRHLRDLMIPLSNVAVLYRTNYQSRAIEEAMLKEGIPYKLVGGFRFYERKE
                     VKDIISYLRFMYNLKDELSLSRILNIPTRKMGPKAVKSLHKLAKECTCTIGEFLVGAF
                     VVLNPEYKHLCDIREISMSKISELQTEFEKLKLVINLFGTLYFQSKEKDVLELIDIIL
                     ERTKYIQWFDDGTEQSDMKKENITELKNVAYTYVSKFGKQSLEMFLNEINLIEQEQDK
                     NQDGSGNYVNLMTLHSSKGLEFDYVFLIGMEEGLLPHSRAFIEEKELEEERRLCYVGI
                     TRAKQKLFLTFAENRLTREGYSTQLPSRFLGEIPQNLCEYFSWNA"
     gene            complement(12290..13471)
                     /locus_tag="PKI16_02765"
     CDS             complement(12290..13471)
                     /locus_tag="PKI16_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53370.1"
                     /translation="MINLIRKMKIPLAILFTIIPTSVFAFSYSKISTLKYDLSLSDQT
                     NTIYATPTTIYLTQVSTQSESLQKNPELWSKALFYYSITKLHFSDMPYNFLIDESGRI
                     YEGRQGGVGANPELKNVDKAIVIGYLSNGSTLTTAASNSLLKMVNDLSTSWGISSYKT
                     VRLQLDAKEGSLSTIVPVDSTGDFAQSVKEALATWKGSSRENLPYKAEIVEIIYEKTV
                     VIGEKLEVTVKVKNMNDFTWLTDRTPIYVSTKDGKDSSFAINGVWDSFSKPTHIEDRA
                     VKPGEIVEFKFKMQPKISPGKVTESFEIMKFDKKPFAGSTFEVTFTVEKGKYTLVKVY
                     SPQYGFANIRECQWYSCKIIESAPEGTVYILKKESDGWMQIQYDEDTIGWVVSRFMKR
                     I"
     gene            complement(13514..>14123)
                     /locus_tag="PKI16_02770"
     CDS             complement(13514..>14123)
                     /locus_tag="PKI16_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53371.1"
                     /translation="NGIPWDEADISAYTFKNFMRKFFAQDWEYKLSQNEIMPNGKEIY
                     YANRTIGENKIETNEYRLETDYLIFQGSKIVGGKLLLTHFTDTGITLPLLGAKDLKKY
                     INLDNYPKEIYPNFSSIDANVLNEIDYLSDEFESLINTLNNCSSGSVETVYLYKNFKQ
                     ESLTPVYKIDLQCEITYKETKYTIPATGYVNAVDPKYISALK"
BASE COUNT         3778 a         2921 c         2121 g         5303 t
ORIGIN      
        1 tcttactgtt tgtagatttc atagtgtaag gtttgtgtgg aaatatttta acccaaactt
       61 ttccttttct atttgtaaaa tttgttatgg atcttcttgc agcttctatt tcccttgcat
      121 taacccaacc attctccata gctttaagcc cgtaatctcc aaagacaatt tcattatttg
      181 aggatgctac tcctcccatt ttacctctga attcttttct gtattttctt ttacgtggct
      241 ccaacatagt tgtttatact aatatattat tcttcagttg ctacttcagt tttatttcct
      301 ttgtatatcc atactttaat acccataagt cctgcatcta ttgttttagc tgtctctgct
      361 gcatagtcta tatctgcttt taatgtttgt gctggtacag aacctgctgt tacttttacg
      421 gttcttgctt gacttgcgtt attgattctt ccagataccc atattcttaa tccttttgca
      481 cctgccatca ttgctttctc ttttgcactc tgaacaagaa gtttggaagg ctgtcttctt
      541 tcaataccgt ctgcaattcc tcttgcgatt attgatgcgg agatatctgc tttctttacc
      601 tctctaatct taacctcaat gtcagctctt gttaatctct ttaattcttt ctgtaatttg
      661 tcaattcctt ctccacctct tccgatagct actcctggtc ttgcaacata gacaattatc
      721 tctactttgt ttgcggatct gtttatttca actctgtcta tccctgcatc tctcattgtt
      781 tctcttatct ttgcctcaat tttaatgtcc tgagcaaaag tctttggaaa atttttcttc
      841 tctgaatacc aagtggcatt ccaccccttg tttattccca ttcttattgc tattggattt
      901 acctttcttc ccatgttatt taacttttaa ttctaaatta agatgtgatc ttcttttggt
      961 gattcttgaa gttctacctc ttgatgcaaa tctaactctt tttagagttc tactttcatc
     1021 tacagatatc ttacttacta ttaattcatc tttgtccaca ctgtataatt ctcttgcact
     1081 tgcgattcca gagagtagag ctttctttac aatatcgcat ccctttttat tcaaaaccat
     1141 taatatatca agagctttgt ctgcctgttt tcctcttact gtatccgcca caagtctaag
     1201 cttctgtgga gactgtgcta tatcttttac atttacttgt actgtcttct cttccatagt
     1261 ggtttaaata tattaattac tcttcaaatc ttcctgagct accatatacc ttagaaagtt
     1321 tacccttctt agcatggcct ctaaattttc ttgtaggaga aaattctccc aatctgtgcc
     1381 ctatcatgga ttcaataact aaaacctctt catgtttttt accgttgtgt actaaaaatt
     1441 tcattcctac catctcagga gttatagtgg atgcccttgc ccatgtttta ataggtgtca
     1501 tcttaccact atctcttgcc atggttactt tcttcattaa tttttcatct gtatatggtc
     1561 ctttttttag tgatcttgcc attctaattg ctcaataatt tatttcttag caaaaggtct
     1621 attcttagtc cttcttgttt taataatgaa cttctctgtc atactgttat gtctagttct
     1681 agttccaatt ctgtgtcccc aaatatcttt agcgtgtctt ccaataacac ctttaccttc
     1741 accaccacca tgtggatgct gtactgcatg cattgccatt cctcttactg ttggtctcca
     1801 tcctaaattt ctctttcttc ctgctttacc caatttaaca tttaccttat cttcatttcc
     1861 tactaaacca attgttgcca tacactctcc atacaccaat ctaatttctc cagaaggcat
     1921 ttttagctgt acatatttgc ctgtagtgtc tggtccttgt acaattattg caccacctgc
     1981 agatcttcca aatgtagcac ctttccctgg gatattttct acacagtgaa caatagtgcc
     2041 agaaggaatg tgctttacca tcatagcgtt accaggtaag atatctgctt tctctcctga
     2101 aaccaatgtg tctccgactt taattccctt aggagcaatg atatatcttc tctctccatc
     2161 ctcatactgt aaaagtgcca aatgtgctgt tctgttagga tcaaagtaaa agccttcaac
     2221 ttttccaggg acaccaaatt tgtctctttt gaagtcaact actcttactc ttctcttaaa
     2281 accaccacct ctatgtctta cggtaatctt tcccatattg tttcttccag atttctgttt
     2341 tttagcaaca gttaatcctc ttggaccttt tgccttctct aaacctgacc tgtccccaag
     2401 tttggttcct ctgcgtgatg aagttgtagg attaaaattt cttactgcca taattattta
     2461 gctaaaaatt catctatctt atctcccttt tttaaggtaa cagtatattt aactgcatct
     2521 gattttctat atgatttgtt tgtcttccaa ttcttaaact gttttcctgc tcttactgtg
     2581 ctatttactt tctctacatg tactttgtac ttttcttcta cagcttttcg catctcaatc
     2641 ttgttaactc ctctatttac taagaatgta tatttattct gtgcattagc tatgttgtaa
     2701 cttttctcag atattactgg ttgtagttga atatttttac tttccatttg ttaatctttc
     2761 ctctaaaagc ttaactacat tgttatctac taatattttc tttgcagaag ctaagtgttt
     2821 tgcatttacc ttgccacctt ctatagtaat aattttttcc atatttctaa gtgcagctat
     2881 tacattttta tcatctgata taaccataat tctgttgtgt ttatctcctt caatatcctt
     2941 tctaatttta tttaattctg ttgcttctat gtttatgaac tccaactctt tgtttcttaa
     3001 tctctcagaa agcattatac atgttgcttt tcttgccata cctttattta ttcttttaga
     3061 ccaatttttg ttgtttggaa caaatgtaac acctccacca acccaaagtg gtgaccttat
     3121 ggagccagat cttgctctac cagttccttt ttgcctccat ggtttaatac cacctccact
     3181 tacatctcct cttgtcttag cattggatgt cccttttctc tcattgctaa agtacactcc
     3241 taatacttga gatactaaat ctccgttgta tggtacttcc caaacttttt tgtttagaga
     3301 aattttcttt tcttcagttg tttttgttac ttttttctct accatattat tctgtaaata
     3361 ttgagactac ttgccccttt ggacctggaa caggaccgga gattagaatg tagttctctt
     3421 tgatatctat tatttcttta tcttttatag ttactgtatc ttgtcccatt cttccaccca
     3481 ttttctttcc tttaagaact ctacctggat cagtacctgc accaatggaa cctggtgctc
     3541 tcaatctatc tgactgacct cttgttcttt gtccaccttt aaatccccat ctttttacta
     3601 cacctgcaaa tcccttacct tttgaagtac ctgttactgt tactttctct cctaccgtaa
     3661 acatttctgc ttttatctca tctcctatct ttacttcctc acctaaatct cctttaaaat
     3721 attttctgaa aagagggatt ctttttgctt ctttgtattt acctgccaat gcttttgtag
     3781 atttcttctt ctctcccaaa cctaattcaa atcccattgc ttctttgttg gaaagaatac
     3841 atccggtaac atccaagaca gtaacaggaa cgatattatc gccctttact acccttgtca
     3901 ttcctttttt tattccaatc aaagctttca ttatttttaa tcaaaaatta ctgtatcgcg
     3961 ataccaactc cagcaggcat ttcaagatgt tgtagcgcat ctatcgtctt gggagtagga
     4021 tctagtatat ctattactct tttatgagta ctaatctcaa aatgttcaat tgagctctta
     4081 taaataaatg gtgatctatt tactgctatt ttctttcttt tagtaggcat aggtattggt
     4141 ccagcgacct tagcgcctgt gcttattgcc gtatctatga ttctcttagc agatttatct
     4201 actactacag aatcataacc tttcaatttt actctaatcc ttgcagtcgt attaaccatt
     4261 ttatttaaaa gagctaaatt aattttaaag agagggtagg atttctccta cccccaatta
     4321 aaccttactt aataatctct gtaacaatcc cttgacctac tgtttgacca ccttctctga
     4381 tagcaaagga aacacctttc tctaatgcta ctggagatat taatttaaca gtaaactcag
     4441 tattgtctcc tggagctacc atctctgtac cttcgttaag aattacttct cctgtaacat
     4501 ccgcagttct aatatagaac tgaggtttgt atcccgttac aaatggtgtc catcttccac
     4561 cttcctcttt gcttagtaca tatacttctg ccttaaactc tgagtgtgtg tttacagatc
     4621 ctggtattgc caatatctga cctctttcaa catcctctct ctttagtcct cttaaaagta
     4681 tacctacatt gtctccagcc tgaccctctt taagagtctt gttgaacatt tcaattcctg
     4741 ttactgcagt cttctgtggc ttttttccca ttccaagtac atcaacagca tctccgactt
     4801 taatgattcc tctttcaatt cttcctgtta caactgttcc tctaccttcg atagagaata
     4861 catcttcaat aggcattaag aatggtttct caatatctct ctctggaagt ggaatatact
     4921 catcaacagc cttcatcaaa tctctagccg cctgaagtcc ctcttcctct ccattttgag
     4981 ctttaagagc atctccaaca attattggac aattttcatc atatccatat ttcttaagaa
     5041 gatcttgtac atctgcttta ataagctcaa gtatctcttc atctgcttca ccaaacttgt
     5101 tgatgaatac caacatctta ggaacaccca cttgctttgc caaaaggata tgctcttttg
     5161 tctgtggcat tgctccatca tttgaggaga taaccaagat tgcaccatcc atctgtgctg
     5221 cacctgtgat catgttcttg atgtaatctt tgtgtcctgg acagtctact aatgcgtagt
     5281 gtctgttctc agtctcaaat tctacatgtg ctactgcgat tgtgattgac tttgctcttg
     5341 aagaaggatc tttgtcaagc ttatcaatca ttttcgcctt ctcatcatca gatgcaaaag
     5401 ttgttgtatg agcattaaga atacctctaa ccaaagttgt cttaccgtga tcgatatgac
     5461 ccaaggttcc aatattcatg tgtggctttg ttctattaaa gtctgccata atatatttat
     5521 aataaattaa ataaatatta ttcttataca agatttgtaa gttttacaaa tctgcgttga
     5581 atattatcaa aaagtaatga tagttgcaag tgcttaatag tcatttagca gtatttttgg
     5641 ctatttcttc ccagaaattt cctcagctac atttcttggc acttctgcat aatgggatgg
     5701 ttccatattt gaacttcccc taccactagt aattgatcgt agttcacttg tatatccaaa
     5761 tagctctgac aatggtacaa atgcagatat cttgctaatt ccgtttccaa tactttctgt
     5821 cccttgaatc tgccctctct tactgctaag cgatcctgtt acatctccca tatattgatc
     5881 tggtgtttct acttcaacct tcatgacagg ttccaaaagt actggatcac ccatcttaca
     5941 tcctgcttta aatgccattg agccagcgac tttaaaggca gcctcagaag agtcgacatc
     6001 atggtaagat ccatcatata cagtcacttt gacatctact actgggtatc cagctaccac
     6061 tccagacttc atggcttcaa caattccctt ttcaattgca ggtataaatt cctttggaat
     6121 agctcctccc ttaattgcgt taacaaactc aaatccttta ccttgaccat ttggttctaa
     6181 tctaatccaa caatgtccat actggccttt accaccagat tgtttaatat atctaccttc
     6241 tgcctctggt acagatttct taatagtttc tctataagca acctgtggtt ttccaacttt
     6301 agcttctaca ttaaattctc tcttcaatct atcaacaatg atttctaaat gaagttcacc
     6361 cattccatga ataattgtct ggcttgtatc ttggtctgtt gttactttaa acgtaggatc
     6421 ttcttttgtt aatcttacca aagcttctgt cattttctct tcatctgact tggtagcggg
     6481 ttcaatagct tcagacacaa ctggctctgc gaagtctatc ttctcaagaa taattggctt
     6541 tgcttcctca caaagagtat ctccagttat tgaatcttta agaccaacaa tagccccgat
     6601 atctcctgct ctcaatgatg gaacctcttc cctgtcatct gcatgcataa ggacaagtct
     6661 tcctactctc tctttaatat tctttgtcga atttaataca tatgtacctg catctatctt
     6721 cccagagtaa actctgaagt atgaaagatt accaatgtgt gggtcattta caattttgaa
     6781 taccaaagaa gagaatggtt cattctcatc tggatgcctt tcaatctctt cacctgtttt
     6841 tggatgaact cctttaactg caggtacatc caatggagat gggagacata ttgtaatgta
     6901 gtctagcaat gtttttgcca tagcggatct agaatcgccc cctaagactg ggaacatttt
     6961 taaccccaag gttgccttcc taagtgcacc atacaattct tctggtgtaa attctccacc
     7021 ttcaaagtac ttgttcataa gttcatcatc ttgctcaacc aatacttcca agagaattgc
     7081 tctgtatttc tctgccttct ctttcatatc ttcagggata tctatctctg ttaactctgg
     7141 ggtatctatt ccatcgtatg tatatgccct cattttgata agatcaatgt atccaaaaag
     7201 ttgctgctct tttccaagag gcagtgtaac tgcaactgct ttgttactta atctcgtttt
     7261 aattgtttca aaactctttt caaaatctcc tccaatcaag ttaatcttgt tcataaaaca
     7321 aatccttgga actttgtact tgttcgcttg tctccatact gtctctgact gaggctctac
     7381 accctttctt ccatcgaaga taaccgcagc tccatctagt actctaagtg atctttctac
     7441 ttctgctgtg aaatctacat gtcctggagt atcgataata ttgattctgt atctcttctg
     7501 gttataatcc cagaaacagg ttactgctgc ggacatgatt gtaattcctc tttcctgctc
     7561 ttgaaccatg aagtctgtag tagcagctcc ttcgtgtact tctcctattt tgtgaacttg
     7621 tcctgtaaag aacaaaagtc tttcagtagt cgttgttttt cctgcatcaa tatgtgcaat
     7681 gataccgata tttctaatca tatctaaagg catcttcaca ttgtaacttg tcttatccat
     7741 cttttttaat ataaaaatta acaatacaaa caaaggaaag ttcccactct cgcagtaggg
     7801 attatatcag aactactata tctgggacaa cagttgattt gcttcttctg taactaagtt
     7861 gtttgtatca tattcaatcg cttttttgag caactccttt gcattgtctt tattacctaa
     7921 attgtattct aattgcccac ttacaaagta atactgggga ttaaacctgt ccaacttcac
     7981 caactcctct aggacttgtg cagccaaatc tgttttccct ttagatagta gattagatgc
     8041 attaagatac atatattctt ttttctctgc atcatttaac ttcatcttgg ataaaagacc
     8101 tatgtagtaa tcaacttttt tagaatcatt tagagctttg ttaaccttta ctccataaag
     8161 gcgaatatat ggcgaagaaa tatagttcat tagtttttta accatcccat ctgctcgaag
     8221 tgtctgatta ttctccaaca aaagatccat atattctgag actaattctt gagagacatc
     8281 ttctcctgca tatgaaattg ctttgtcgta gctactaaaa gagttctcta agtcatcttt
     8341 aagtaaatat gcccttgctt gtccccaaag aatatcttct tccattccac ccaaagagag
     8401 tgccctgtca aactgtgtta ttgctttgtc atactctcca gaatccagat atgctcgacc
     8461 aaggaaatat actccttcaa gatattcagt aatgtcttca gaaagaggct ctagcaaatt
     8521 aaccgcaagt acgggatatc cactattaat atatacccta gataatttag tagcgttgaa
     8581 ttttgaatct tctgtaagac tttcaagtat ctcagagaat tcatcgtaga atgccttcca
     8641 tgtatcagag ggttgtatgg aacctaacac ctttttagca tcatctatat tggtagtggc
     8701 aagaatgtat gcacgcaaaa gagtcgattc gtacaaagta tcttctgtgt aaatggattt
     8761 ggagagatat gtattggcat catttaaatt cccttttttc aagtatgttt tagcaatact
     8821 aagctcaagc tctggatttg tcattccgac tcctaatcca tccaagtaag tactcaacgc
     8881 ctgatcatag agagaatctt cataatattt gttccctatt ttcaaataca agatagattt
     8941 atcatagtta ctaatattct tcgcggattt ctcgacaatc tcaactgcat ctgatgttct
     9001 gttttttaac aacagaatat ctactatccc ttcgtaagcc tcaactctac ttggtataac
     9061 atctgctgca tcgtagtatc tgttaattgc cgtagaatac tctctagcct catactgaga
     9121 atctgcttca gagataattt gttggtaaga tgtaatttgc tcctgggtta tccttttgct
     9181 aagtaaatac cagatacccg ttcctagaag aattactgcc aatacaaacc atggagcaag
     9241 cttttttagt ttctctttca ttaattgaat atagattagg aataaataca tgtcaaatat
     9301 tttcttacat atatgttaaa ataggctaat ttatgtttaa gactagatta attggaattg
     9361 tgctcgctat tgcgctcatg tttgtgatga gtctaattcc aacggctaat tggacagata
     9421 aagaagcaag gtacagaagt aatactctaa tacacatggt atacggtatt gatgctgatg
     9481 tagagaaagg gagatatact ccaaaggttg tggttgtaaa ctacgaaggt gagattaaga
     9541 agcacatatc tacggcttct tcccctgttt tcgctttgtt agagttaggg taccctatta
     9601 gtacaatgaa aaaggttgta agtacttcac caatgaacac tctttacaac aattcatata
     9661 tatatgtctt ctcctacaga gataccatag acgaggttaa attagaaatt ccataccaaa
     9721 gaattgtgaa aggtggcact ctttgtgaga gattaagtca gaaagttctg caacaagagg
     9781 gagttcttgg aataatgaca cagaaggtaa gaagagtgta cgagcgagga gaattggtta
     9841 tggaggagat attagagaaa gatattacaa gagagccaag agcagagata tatataatca
     9901 aaggtccaga cgattctcca aaggaggttc cacaaattgg aaataactgt gactactggt
     9961 ttgcttatgt agataccatt aaagcttccg aagaagagaa gagatggtta aaatggacaa
    10021 tgaaatatga atccggttgt aatgcagaaa acaacaggca tccatattac aaaggtttgt
    10081 tccaatggga tccatgcctt tggtatcaac agtatccaaa tgacaatatt ttcgatggta
    10141 gagctcagat taaaaggact ttggaaaaag tcagaggagg agcaaaccct gcaagaatgt
    10201 ggccggctgt ttacaagctc tatgttgata aatatggaga gctgagttgg ctttaagcat
    10261 tccagctgaa atattcgcaa aggttttgtg gtatttctcc aagaaatctc gaaggtagtt
    10321 gggttgaata tccttctctg gttaacctat tctctgcaaa tgtaagaaat agtttttgct
    10381 tagccctagt tattcctaca taacaaagtc ttctttcttc ttctaattct ttctcttcaa
    10441 taaatgctct tgaatgagga agtagtccct cttccatacc aattaggaat acatagtcaa
    10501 attctaagcc tttagaagaa tgaagggtca taagattaac ataattacca cttccatctt
    10561 ggtttttgtc ctgctcctgt tctattagat taatttcatt taaaaacatt tctaaactct
    10621 gcttaccgaa ctttgataca taggtatatg cgacattctt aagctctgtt atattctctt
    10681 tcttcatatc agattgctct gttccatcgt caaaccactg aatatattta gtcctctcta
    10741 ggataatatc tattaattca agaacatctt tttctttaga ttggaaatat agtgtgccaa
    10801 agagattaat aactaatttc aatttttcaa actctgtttg taattcagat attttagaca
    10861 tagaaatctc tcggatatca catagatgtt tgtattctgg attcaggact acaaatgccc
    10921 cgacaagaaa ttctccaatt gtgcatgtgc attcttttgc taatttatgc agagatttaa
    10981 cagcttttgg acccattttc ctggttggaa tatttaaaat tctcgaaaga cttaattcat
    11041 ctttaaggtt gtacataaaa cgaagataac taattatatc tttaacctct tttctttcgt
    11101 agaacctaaa tcctcccact aatttgtaag gtataccttc tttcaacatt gcctcctcaa
    11161 ttgcacgaga ttggtagttt gtcctgtaaa ggacagcaac attacttaga ggtatcatta
    11221 ggtctcttag atgtctaatg ttttcaacaa catactctgc ttcttcttct tggtctcttg
    11281 cttggtacac agtaattgtt tctccttggt tattttctgt ccaaagattt tttacaatcc
    11341 tctggttgtt ttgttgtata acagagaccg ctgcctctat gacatttttt gtagatctgt
    11401 aattttgttc tagtttaata actgttacat ttttaaaatc tttttcaaat gattgtatat
    11461 ttttaatatc tgcacctctc catgcatata ttccttggtc atcatctcct acaacacaga
    11521 tgttttggtg tttttcagag agtaatttcg caaatctgta ttgagcattg ttagtatctt
    11581 ggtattcatc tatgaggata tatttatatt tgtcttggta atattttctt acttcctcat
    11641 tttcttctag catttttaca gtaagaaaaa gaaggtcacc gaagtcaaca gaattttgtg
    11701 ctttaagttg gttttggtat acagggtata tctgtgcagc gatatcttct atgtacccac
    11761 caaaatggtt agagaattgt gctggtgtta ccatatcgtt tttggcagct tctatgaagt
    11821 gtgcaattgc cttcggttta atctgtttaa tatctatctg catctcaagc ataatttctc
    11881 ttatgaggtt ttcagaatca tctgaatcat atatggagaa attgtggagt aatccaactt
    11941 cttgggcttt tcttcttagg agtgttgccc caatggagtg aaaggttcca atcattggaa
    12001 ggtctgtaac atctctattt aaatctttga gaagttttct aactctctcg agcatctctc
    12061 cagaagcttt ttttgtaaat gtaacggcaa ggatattttc tggcttaaca ccttttactg
    12121 ctattaggta agcaatcctg tttgttatga ctcgtgtttt accagagcct gctcctgcaa
    12181 aaactagaag aggtccctct gtacacataa cagctttttt ctgttgggga tttagcttcc
    12241 caagtattat gtcttgttcc acaactgtat gtattaagta aatctagtac taaattcttt
    12301 tcataaatct ggaaactacc catccaatgg tatcctcatc atactgtatt tgcatccatc
    12361 catctgactc ttttttaaga atatatactg taccttcggg tgcgctttct ataattttac
    12421 agctatacca ctgacactct cgtatgttag caaacccata ttgaggagaa tataccttca
    12481 ccaaagtata tttacctttc tctactgtaa atgttacttc aaacgtggat ccagcaaaag
    12541 gttttttgtc aaatttcata atttcaaaac tttcagtgac tttccctggg ctaatctttg
    12601 gttgcatttt aaatttgaat tctacaatct cccccggttt aacagccctg tcttctatgt
    12661 gggttggttt actaaaacta tcccagacac cattgattgc aaaactactg tcttttccat
    12721 ctttggttga tacatatatt ggggttctgt ctgttaacca tgtaaaatca ttcatatttt
    12781 taactttaac tgtaacctcc aatttctctc caattacaac agtcttttcg tatatgattt
    12841 ccactatctc tgctttgtat ggaagatttt ctctactaga gcctttccat gttgcaagtg
    12901 cttcttttac tgattgagca aagtcacccg ttgaatctac tggaacaatt gtgcttagag
    12961 agccttcttt tgcatctagt tgtagcctta ctgtcttgta tgaggatatt ccccagcttg
    13021 tggagagatc gttaaccatt ttaaggagtg agttacttgc cgcagttgtg agggttgaac
    13081 cgttggaaag gtatcctatg acaattgctt tgtcaacatt ttttaattct gggttagctc
    13141 ctactcctcc ttgtcttcct tcatatatcc ttcctgattc atctatgagg aaattgtatg
    13201 gcatatccga gaaatgtaat tttgttattg agtagtagaa aagagcttta ctccacagct
    13261 ctggattttt ctgcaatgat tctgattgag tggatacttg tgttaaatat atggttgtag
    13321 gggttgcata tattgtattg gtttggtctg agagcgaaag gtcatattta agagtagaga
    13381 ttttactgta gctaaatgca aaaacagatg taggaataat tgtaaaaaga atagcaagtg
    13441 gtattttcat ttttctaatg aggtttatca ttatcttatt ctatcattat atggtaaatt
    13501 ttttctaatt cttttacttt agggctgaga tgtactttgg atctactgca tttacatatc
    13561 ctgttgctgg tatcgtatat ttagtttctt tatatgttat ttcacactgc aggtcaattt
    13621 tgtatacagg ggttagtgat tcttgtttaa agtttttgta tagatatact gtctctacag
    13681 aaccacttga acagttattt aaagtattta ttaaagattc aaattcatcc gagagataat
    13741 caatctcatt tagaacatta gcatctatag aagaaaaatt aggatatatt tctttaggat
    13801 agttatctaa atttatatat ttttttagat cttttgctcc aagcaaaggt agggttattc
    13861 ctgtatctgt aaaatgtgtt aagagcagtt tccctcctac tattttactt ccctgaaaaa
    13921 ttaaatagtc tgtttctaat ctatattcat tagtttctat tttattctca ccaatagttc
    13981 tgttcgcata atatatctct tttccattag gcattatttc attttgagaa agtttgtatt
    14041 cccaatcttg tgcaaagaat tttctcataa agtttttgaa tgtatacgca gagatatcag
    14101 cttcatccca tgggatacca ttt
//
LOCUS       DAOQWP010000018        12613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_5472_length_12613_cov_6.483755, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000018 DAOQWP010000000
VERSION     DAOQWP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12613
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_12613_cov_6.483755"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PKI16_02775"
     CDS             complement(<1..360)
                     /locus_tag="PKI16_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53372.1"
                     /translation="MPKSKMLVLVAGVVVLLGAAVAVYFFFLKERDYIADAPKGCPKT
                     SSIIVTSEEAGTQKITSENSNFIHWDKSQGMLVFTNYTLNVDSVYSDITGEHVLAVIK
                     LKNNDSTEIGLGTYRKNP"
     gene            449..1495
                     /locus_tag="PKI16_02780"
     CDS             449..1495
                     /locus_tag="PKI16_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNR53373.1"
                     /translation="MIREIEDKTAWDTYLNQCQKKTFLQTYEWGEFQKDLGKNIMRFA
                     FFNENEILLGISLCIFEKTPFGTQIYLPRGPLMTIETPETYKSILNALTEYFKDKNIS
                     YIKIDPAFTEKEEIAKVPQSIGFKHSINFVQSETNWMVDLVGNTEEELLTWCKEHDMS
                     KNYPAYIRKARREGIDISFSNNLEDWKLFHTYLQKSGERKSFDVRPLSYFTSLHKYFS
                     QSGVVRLGVAKRNGNILAMILIAVYGDEISCLYSAQTDIDTKLRAPMLLRWECMLMGQ
                     REGIKRFNNWGVLPEERYKPGNPGFGYSQYKRGFGGYLEQIERTYEYVYNKKLYPIQR
                     LYDRYIKFRYYRYR"
     gene            1541..2827
                     /gene="hisS"
                     /locus_tag="PKI16_02785"
     CDS             1541..2827
                     /gene="hisS"
                     /locus_tag="PKI16_02785"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNR53374.1"
                     /translation="MNINVLPGFQELLPKEQIAFDRLKRTIEDIYQLYGFRPIETPVL
                     ERAEILLSKSGGETEKQIYQFEKGDTKLAMRFDLTVPLARYVVKNQNDLVFPFKRYAI
                     DRVYRGEKPQAGRFREFYQCDIDVIGRDTLDLSYDAEIPSIIASIFSSLELDFTIRIN
                     NRKILNGLFDLLKIEDRVSVLRTLDSMEKIGKEKIKAELKEIGLEGSQIEKIFDFYKL
                     NEKDNKEIINSLKDLDTSSDIFKKGVEELEIVMNEINTLGISSNVKIDLTVARGLDYY
                     TGTVYETILNKYPEIGSVCSGGRYDDLAGYFSNQKFPGVGISIGLTRLFDQLKDRDVF
                     CIEDETPTAVLVLPLSDEREISLKLADTLRENFISTEIAPSGNMKKALNYANKLKIPY
                     VIFIGEDEIKKNKYTVKNMITGEQKSVPQANILDIF"
     gene            complement(2834..4006)
                     /locus_tag="PKI16_02790"
     CDS             complement(2834..4006)
                     /locus_tag="PKI16_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNR53375.1"
                     /translation="MRSVGTVVRGIRTPIIKYGDNLENVVVDSLLQAAKNERFQLHDK
                     DVIGVTEAVVAISQGNYVTLEQLVKEFKKLYPKKIGVVFPILSRNRFSLVLKAITMAC
                     KEVHILFSYPSDEVGNHLVEPELVDEKGVDPHSDVLEEDEFRKLFGKKLVHRFTGVDY
                     VQAYKDLGNGNVFAHFSNNPKTILNYTRDVLVADIHTRFRTKKILLEAGAETLYGLDE
                     ICNTPSKAHGYNVDYGLLGSNKTGEEKLKLFPRDCNDLVNRIQKRIKEETGKDVEVIV
                     YGDGAFKDPVGKIWELADPVVCPGFTEGLVGTPKEVKLKYISENWDSKGNLNEYVKKM
                     IKVKNGADYSIEKSLGTTPRRLTDLLGSLCDLTSGSGDKGTPVILVQGYFDDYTVG"
     gene            complement(4029..5372)
                     /locus_tag="PKI16_02795"
     CDS             complement(4029..5372)
                     /locus_tag="PKI16_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNR53376.1"
                     /translation="MPNSKYDVLVVGAGPAGMFACYELLKKRKKRKIGLIDMGHRIEK
                     RKPSEVMSGFGGAGTFSDGKLHFTATLSHEKAFHLIDSKDYQDILEYVDTIFTKFGVG
                     AEYFPKETEDLEKLKDEAMVNDIELVVRKARHVGTDKLKEVMLKFEKYLLEKGVDILE
                     NTEVVDIIVKDGCIKGVITKDGKKIYCETVLLAPGRIRASWLQKLATKHNIPYIYDKV
                     EVGVRVEFPTSIMRRQAEALYEMVYRVRTKTYHDIVRTFCSCPNGLVAKEEYEGYICV
                     NGHSNSDHLSKNSNFALLCEVNLTEPVENSIAYAKSIAEVASTIGGGKPILQSLEDLN
                     SGRRSTWSRLKKSFVSPSLVDVTPGDIAMALPYRIVKNIQEGLEILDTVMPGIHSGST
                     LLYAPEVKFRSSKISTDKGLQTCIKGLYVAGDGAGLSGAITGAAVTGIMFARGLS"
     gene            5468..6085
                     /locus_tag="PKI16_02800"
     CDS             5468..6085
                     /locus_tag="PKI16_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNR53377.1"
                     /translation="MLKKHTKGFNKVVLFLTMADVFVWGPFFIISALSGIYLSNKLGT
                     GTIEFVGVGTAIYFLTRAITQLPIGYITDKIKKDKDEIIILIVGILLMGLPYILYPQI
                     STPMHYYILQFVFGVGVSLNITNWRKLFAMNIDSGREGRQYSFYETIVSLSTVILSAT
                     VGLIANLGDIYFDLVMIASGILMMAGSIWVVLLFKLPKRKTNHIK"
     gene            6127..7290
                     /gene="nifS"
                     /locus_tag="PKI16_02805"
     CDS             6127..7290
                     /gene="nifS"
                     /locus_tag="PKI16_02805"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNR53378.1"
                     /translation="MNQIYLDHAATTKLRKEVLDSMLPFLTEQYGNPSSIYTLGREAK
                     KAIDIAKEQVAKALNAKPNEIFFTGSGTESDNWALKGIAYANKEKGKHIITSSIEHHA
                     VLHTCEFLQKEGFDITYVPVDSEGIIDLDELKKAIRPDTILISVMLANNEIGTIQPIQ
                     EISQIAKENEIYLHTDAVQAVGSISVDVQDLGVDLLSLSGHKIYGPKGVGVLYVRSGT
                     KISSFVHGGGQERGKRAGTENVANIVGIGKAIELATKDISTYSKHLQELRDMTIENIL
                     KEIPHTRLNGHREKRLPGNVNISFEYIEGESILLMLDSKGIAASSGSACTSGSLDPSH
                     VLLAIGLPHEIAHGSLRLTFGEENTKEDVKYLLETLPSIIEGLRKMSPLYNQK"
     gene            7293..7676
                     /gene="nifU"
                     /locus_tag="PKI16_02810"
     CDS             7293..7676
                     /gene="nifU"
                     /locus_tag="PKI16_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HNR53379.1"
                     /translation="MEEYSQKVMDHFMNPRNVGEIENADGVGTVGNPVCGDIMKMYLK
                     IENNVIKDAKFKTFGCGAAVATSSMATELIIGKTLEEALDITNSTVAEALDGLPPVKM
                     HCSNLAQQAVKAAIDDYKKKNEKTK"
     gene            7660..8730
                     /gene="mnmA"
                     /locus_tag="PKI16_02815"
     CDS             7660..8730
                     /gene="mnmA"
                     /locus_tag="PKI16_02815"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNR53380.1"
                     /translation="MKKQSKGKIMIGMSGGVDSSVCAALLKEQGYDVIGVTMQIWQEN
                     KEENGTCCSLTAVDDARKVANVLDIPYYVLNFKKSFDEKVIQYFISEYLQGRTPNPCI
                     ACNKYIKFEELLNKALAMDIPYIATGHYATVENKKGRYLLKKSKSKTKDQTYVLYNLT
                     QEQLSHTVFPLGKYDKNKVRELAKKYNLPVANKKDSQEICFVEDNNYGKFIEENSDSD
                     LVPGDFVNTKEEVIGKHKGIYHYTIGQRKGLGITSNKALYVVDIDIEKNIVMLGEESD
                     LQKKELIAYDLNWILIDKLEKKMNIKAKIRYGAKESPATIYPLEKDKVKVIFKKPQRA
                     ITSGQAVVFYKGDYVVGGGTIE"
     gene            complement(8720..9400)
                     /locus_tag="PKI16_02820"
     CDS             complement(8720..9400)
                     /locus_tag="PKI16_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53381.1"
                     /translation="MNKKVSIIIMAVLFTGTAIFTFTVFYPTLFDSLFVNTQDITVLQ
                     ANASEEIQSSDLGELLDLKVNTPTTVSFELSNRYLPSGWEKKNMYFKYTYLGEYSNSV
                     YSNLNELLKTDYSWRKQLNKQGDIVLAGPMFLKDGIYQLHVHNGLSLAKKYYLFGELL
                     DYLNNKDLLLGTVMKFGDVRLECTTVQEMEIEKGSNISGQMDLVISTCLERNGDLRLV
                     SGWKVITQ"
     gene            complement(9400..10422)
                     /locus_tag="PKI16_02825"
     CDS             complement(9400..10422)
                     /locus_tag="PKI16_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53382.1"
                     /translation="MKDKLLSFVVLPSIMFALFLAFSPVGRVEAQAEKNREICHARSA
                     HSNPYGPKKEYVDDDSIVGDSGHDGHNGPVWFEGITESWGDIIKPFDYGDGQHYAGKN
                     WTTEGQAIFNNNCQPVVVAIRSDFNLTSMCRPSETEGRLRVRNQSDYDYPYVLTLYGT
                     DTKFNGTAPADTDTLVDVPWTDSSNTWILEIAGFSFRKAIGNNALCVPVMCKWDGELE
                     ADDPACPVDCQYDATISSIDESCKAPVMCKWDGELEADDPACPVDCQYDSTISSIDPL
                     CAPKDDDPDTEGVQDDTTKKDTGVVLAATGPTDNTVVYMVELLLVAMLVTYSVFFTKT
                     YLKNSK"
     gene            complement(10570..11052)
                     /locus_tag="PKI16_02830"
     CDS             complement(10570..11052)
                     /locus_tag="PKI16_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53383.1"
                     /translation="MEKEKNNSSSLDIRLAKVREVTKEWQCCTLRYNDLAPYKKTGCE
                     YMELSHEEVDAILPLFSKVFKQEIEVSRFNILSISDEKYFKFGIEDGEGLVDGKECRF
                     KYLLVHNGGLPSIQVVCKDSSGKGIDHEFFPVFSVSCMPEESQEFISSVLDIVEYRLE
                     "
     gene            complement(11054..11530)
                     /locus_tag="PKI16_02835"
     CDS             complement(11054..11530)
                     /locus_tag="PKI16_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53384.1"
                     /translation="MNPTIETEHTSVEVLEGNLKQIEELLMKNVEDPMLVSFYPDMSE
                     TQEINDVLLSVGFLQENMRLHNFHLIATIEPEESFEFGLYGGEGSVQESSKRITCFFH
                     YINSKKEGNIFIRSLQEEGKKSITHTHCLFKDKLDRLSNTDKKIFEVLTSKFLEIK"
     gene            11858..12043
                     /locus_tag="PKI16_02840"
     CDS             11858..12043
                     /locus_tag="PKI16_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53385.1"
                     /translation="MEKPFTIFDIFVMLPLLLFFVTLALAFFDVIDDSFVKQSLLLTI
                     LTPIFILIGGLLQPRET"
     gene            12144..>12613
                     /locus_tag="PKI16_02845"
     CDS             12144..>12613
                     /locus_tag="PKI16_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNR53386.1"
                     /translation="MTQTSKLFTFILLFISSFLIASYFLFSKYLDSDVKQEREEIIQL
                     KNSETLLSQMTLEEKVGQMFLMGFWGTEPDYYITKMIKERHIGGVILFKYNVKDREQL
                     TQLSKNLQLLSDKVPLFVSIDQEGGIVSRLGTDIVSEITAQSEIKTEKDAYNIS"
BASE COUNT         4330 a         2296 c         2092 g         3895 t
ORIGIN      
        1 aggattcttt ctgtatgtcc ctagacctat ctctgtagaa tcattattct tcaacttaat
       61 tactgctaaa acatgctctc ctgttatatc agaataaacg ctatccacat ttaaagtata
      121 attcgtaaat accaacatcc cttgagattt atcccaatga ataaaattgg aattttccga
      181 agtaattttt tgggtacccg cctcttcaga tgtaacaata atagaggatg tttttggaca
      241 accctttgga gcatctgcga tataatctct ttcctttaag aagaaaaaat acacagctac
      301 agcagctcct agaagaacaa ctactcccgc aacaagaaca agcatctttg acttgggcat
      361 attatttaga ataaaaataa aatatagata tattcatatt caaatatagg atgtgaaaag
      421 tcaactgaat atagtaatat aataaaatat gattagagag atagaagaca aaacagcttg
      481 ggatacatat cttaaccaat gtcagaaaaa aacctttcta caaacatatg aatggggaga
      541 atttcaaaaa gatttaggga agaatataat gagattcgca ttttttaacg aaaatgaaat
      601 tcttctgggt atatctcttt gtatatttga aaaaactccc tttggtactc aaatatacct
      661 tcctagaggg cctctaatga ccatagagac accagaaaca tacaaaagta tacttaatgc
      721 tctaacagag tattttaaag acaaaaacat atcatatata aagatagatc ctgcatttac
      781 agaaaaagaa gagattgcaa aagtaccaca aagcataggt tttaaacaca gtattaactt
      841 cgtacaatcc gagaccaact ggatggtaga tttagtagga aataccgaag aggaactact
      901 tacatggtgc aaagaacacg acatgagtaa aaactatcct gcatatatta gaaaagccag
      961 aagagaaggg atagatatat ctttttcaaa caatttagag gattggaagc tcttccatac
     1021 atatttacaa aaaagtgggg agaggaaatc tttcgatgtt agacctcttt cttactttac
     1081 atctttacac aagtatttct ctcaaagtgg tgtcgtaaga cttggagtag caaaaagaaa
     1141 tggaaatatt cttgctatga tactaattgc agtgtatgga gatgaaatat cttgtctgta
     1201 cagtgcacaa acagatatag atactaaatt aagagcacca atgttgttaa gatgggagtg
     1261 tatgttaatg ggacagagag aaggtataaa aaggttcaat aattggggag tattaccaga
     1321 agagagatac aaaccgggta acccaggatt tggatattct caatacaaga gaggatttgg
     1381 aggatatctt gaacagatag agaggacata tgagtatgta tacaacaaaa agttatatcc
     1441 gatacagaga ttgtatgaca gatatataaa gtttagatat tacaggtata gataatcgct
     1501 ttttgacaat atcttccaat taaaatagaa tgtaaacatt atgaatatta atgtattacc
     1561 tggttttcaa gagcttttac ctaaagaaca aatagctttt gacaggttaa aaagaacaat
     1621 tgaagatata taccagttat acggttttag accaatagaa acaccagtac tcgaaagggc
     1681 agaaatactt ctttcaaaga gtgggggaga aactgaaaaa cagatatatc agtttgagaa
     1741 aggtgacact aaattagcca tgcgctttga tttaacagtc cctttagcaa gatatgtcgt
     1801 aaaaaatcag aacgatcttg tttttccatt caaaagatac gctatcgaca gagtatacag
     1861 aggagaaaaa ccacaagcag gaagatttag agagttctac cagtgcgaca tagatgtaat
     1921 aggtagagat acacttgatc tttcctacga tgcagaaata ccttcaatca tagcaagtat
     1981 cttctcttca ctagaattag acttcacaat tagaataaac aacaggaaga tattaaatgg
     2041 tctctttgat ttgttaaaga tagaagaccg ggtttctgtt ttgagaacac tagacagtat
     2101 ggaaaagatt ggcaaagaga aaataaaagc tgaattaaaa gagattggtt tagaaggtag
     2161 tcaaattgaa aagatttttg acttctacaa attaaacgag aaagataaca aggagattat
     2221 taattcctta aaagatttag atacctcttc agatattttt aaaaaaggtg tagaggagtt
     2281 agaaatagtt atgaatgaga ttaatacatt agggatatct tccaatgtaa agatagactt
     2341 aacagtggca agaggattag attactacac gggaactgtt tacgaaacaa ttcttaataa
     2401 atatcctgag attggttctg tatgtagtgg tggaagatat gatgacttag cgggttactt
     2461 ttcaaaccaa aaattccctg gagtagggat atccataggg ttaacaagac tattcgacca
     2521 attaaaagac agggacgttt tctgcataga ggatgagacc ccaacagcag tattagtact
     2581 tcctctttca gatgagagag aaattagttt gaaattagca gatacactaa gagagaattt
     2641 catatctact gagattgctc catctgggaa tatgaagaag gctcttaatt atgcgaacaa
     2701 attaaaaata ccgtatgtca tatttattgg agaagatgag attaagaaga acaaatatac
     2761 agtaaagaat atgataactg gagaacagaa gagtgtccct caggcaaata tactagacat
     2821 attttagagt cccttatccc acagtatagt catcaaaata tccctgtacc aatataactg
     2881 gggtcccttt atctccactt ccacttgtta aatcacatag acttcccagc aaatccgtaa
     2941 gtcttctagg agtagttcca agagatttct caatggaata gtctgctcca ttttttacct
     3001 taatcatctt cttaacatat tcatttaaat ttcccttact atcccaattt tctgaaatat
     3061 attttaattt cacttccttt ggagtaccaa ctaatccctc tgtaaatcca ggacatacaa
     3121 caggatctgc caattcccat attttgccta cgggatcttt gaaagctcca tccccatata
     3181 caattacttc aacatctttt cctgtttctt cttttatcct tttctgtatt ctgttaacca
     3241 aatcattaca atctcgtgga aaaagcttta atttctcctc tcctgtttta tttgaaccca
     3301 aaagaccata atctacattg tatccatgag ctttacttgg ggtattgcat atctcgtcta
     3361 gtccatacaa cgtttctgct ccagcttcaa gtagaatttt cttagttcta aatcttgtat
     3421 gaatatctgc caccaataca tctcttgtgt aatttaagat tgtttttgga ttatttgaaa
     3481 aatgtgcaaa tacattacca tttcctaaat ctttgtaagc ttggacataa tcgacaccgg
     3541 taaatctatg cactaatttt ttgccaaaca attttctaaa ttcatcttct tctaaaacat
     3601 cactgtgtgg atctacacct ttctcatcta ctaattccgg ttctacaaga tgattaccta
     3661 cctcatctga tggataacta aacagtatat gtacttcttt gcatgccatg gtaatagctt
     3721 taagtaccaa agagaatctg tttctactta agatagggaa tacaacacca atttttttag
     3781 gatacaattt cttaaactct tttaccaact gctctaaggt gacataattc ccttgggata
     3841 ttgccaccac agcctcggtt actccaatta catccttgtc atgtaattga aatctctcat
     3901 tttttgctgc ttgtagaaga gaatctacta ctacattctc aagattatcc ccatatttaa
     3961 tgattggagt tcgaatacct cgcacaacgg ttcctactga tctcattggt atatatataa
     4021 aaatttaatt aggacaaacc tctagcaaac ataatacctg taactgcagc acctgtaatt
     4081 gcaccagaca atccagcacc atctcccgcg acatagagac ctttaataca ggtttgcaat
     4141 cccttgtccg tagatatttt agaggatcta aacttaacct caggagcata gagtagggtt
     4201 gaacctgaat gaataccagg catcactgta tctagaatct ctaacccttc ctgaatattc
     4261 ttaactattc tgtatggtag tgccattgca atatctccag gggtaacgtc aacaagagag
     4321 ggagatacaa aagatttttt aagtctactc catgtacttc ttcttccact atttaaatct
     4381 tctaaactct gcaagattgg tttaccccca ccaatggtgg aagcaacctc tgcaatggat
     4441 ttggcatatg ctatggaatt ctctactggc tctgttaaat taacctcaca tagaagtgcg
     4501 aaatttgaat tctttgaaag atgatctgag ttggaatgac cattaacaca tatatatccc
     4561 tcatattctt ccttagctac aagaccattt ggacaactgc aaaaagttcg gacaatgtcg
     4621 tggtaggttt tagttcttac tctgtacacc atctcataga gtgcttctgc ctgtcttctc
     4681 atgattgaag taggaaattc tacccttact ccaacctcta ccttgtcata tatatacggg
     4741 atattgtgtt tagtagctaa cttttgtaac cagctagccc ttattcttcc tggagcaagt
     4801 aaaactgttt cgcaatatat tttctttccg tctttagtga taacaccctt tatacaacca
     4861 tctttgacaa taatgtctac aacttcggta ttctctagaa tatctactcc tttctctaaa
     4921 agatactttt caaatttcaa catcacctct tttaatttgt ctgtgccaac atgtcttgct
     4981 ttccttacaa ccaattcaat gtcatttacc attgcctcgt cttttaactt ctctaaatct
     5041 tctgtttctt tcgggaaata ttctgcacct acaccaaact tcgtaaaaat tgtatctaca
     5101 tactccaaaa tatcttggta atccttactg tctattaagt ggaatgcttt ctcatgggag
     5161 agcgtggcag tgaaatgcag tttaccatct gagaatgttc ctgcacctcc aaatccagac
     5221 attacttcgg agggttttct tttctcaatc ctatgaccca tatctatgag tccaattttt
     5281 cgtttctttc ttttctttag aagttcatag caggcgaaca ttcctgcagg accagcacca
     5341 acaactagca catcatactt agaattaggc ataggggtct gttacggtta ggttacatat
     5401 tattattcca gaagaaaaag tttttgacaa gttttttaat tttttaaatt gtgataccat
     5461 tgtctagatg ttaaagaaac ataccaaagg ttttaacaaa gtagtgctgt tccttactat
     5521 ggctgatgtt tttgtatggg gaccattctt catcatatct gcactttcag ggatatatct
     5581 ctctaacaaa cttgggacag ggaccataga gttcgtagga gttggaacag caatatattt
     5641 tttaacaaga gcaattactc aacttccaat tggatatata accgacaaga ttaaaaaaga
     5701 taaagatgaa ataattattc taattgttgg cattcttctc atgggacttc catatatttt
     5761 atacccacaa atctccacac ctatgcatta ctacattctt caatttgtat ttggagtcgg
     5821 agtttcttta aacataacaa actggagaaa actttttgca atgaatattg acagtggaag
     5881 agagggaaga cagtacagtt tctacgagac catagttagt ttgtcaaccg taatacttag
     5941 tgcaacggta ggattaatag caaacttagg cgacatatat tttgatttag taatgattgc
     6001 ttctggaata cttatgatgg ctggtagtat atgggtagtc ctattattca agcttcctaa
     6061 gagaaaaaca aatcacataa aataatccca cgataaatat ttgactcaga tgatataatt
     6121 acatctatga accaaatata tttagatcac gctgctacaa caaaattaag gaaagaagta
     6181 ctggattcaa tgcttccttt tttaacagag caatacggaa acccctcctc catatatact
     6241 cttggtagag aagctaaaaa agccatagat attgcaaaag aacaggtagc caaagctctc
     6301 aatgctaaac caaacgaaat cttctttact ggttcgggta cagagtcaga caactgggcc
     6361 ctaaagggga tagcatatgc aaacaaagag aaaggcaaac atatcataac tagtagtatt
     6421 gaacatcatg cagtactaca cacatgcgaa tttctacaga aagaaggctt tgacattaca
     6481 tatgtccctg tagacagtga aggcataata gatttagatg aattaaagaa agccattagg
     6541 ccagacacca tcctcatctc tgtaatgctt gcaaacaatg agataggaac aatacaacca
     6601 attcaagaaa tatcccaaat agctaaagaa aatgaaatat atttacacac agatgctgtt
     6661 caagcagtag gaagtatttc agtagatgta caagatttgg gagtagattt actctctctt
     6721 tctggtcaca agatatatgg accaaaggga gtaggagtac tttacgtaag aagcggaaca
     6781 aagatatctt cttttgtaca cggtggtgga caagaaagag gtaaaagagc agggacagaa
     6841 aacgttgcaa atattgttgg aattggcaaa gccattgaac ttgcgacaaa agatatatct
     6901 acatacagta agcatttaca agaattaaga gatatgacca ttgagaatat acttaaagaa
     6961 atcccacaca caagactaaa tggacacaga gagaaaagac tccctgggaa tgtaaacata
     7021 tccttcgaat atatcgaggg tgaatccatt cttctcatgc tagactctaa aggtatagct
     7081 gcatctagtg gctcagcatg tacctccggt tcactagatc cttctcatgt gcttcttgcc
     7141 ataggtttac ctcacgagat agcacatggt tctctgaggt taacttttgg agaagaaaat
     7201 actaaagagg atgttaaata tcttttggag acattaccct ctattattga agggttaaga
     7261 aagatgtccc cgttatataa tcaaaaataa atatggaaga atatagtcaa aaagtaatgg
     7321 accatttcat gaatcctaga aatgtaggag aaattgaaaa cgccgatgga gtaggtacag
     7381 ttggtaaccc tgtgtgtgga gatattatga agatgtacct taagatagag aacaatgtaa
     7441 taaaagatgc gaaatttaaa acatttggat gtggtgcagc agtagctaca agtagtatgg
     7501 caacagaatt aataattgga aaaacactag aagaagcact agacattaca aacagtacag
     7561 tagcagaagc tttagatgga ctaccacctg ttaaaatgca ctgctctaat ctagcacaac
     7621 aggctgtaaa agctgcaata gatgactaca agaagaagaa tgaaaaaaca aagtaaaggg
     7681 aaaatcatga taggtatgag tgggggagta gatagctccg tatgtgcagc acttcttaaa
     7741 gagcaaggat acgatgtcat aggagttact atgcagatat ggcaggagaa caaagaggag
     7801 aatggtacat gttgctctct tactgcagta gatgatgcaa gaaaggtagc aaatgtccta
     7861 gatattcctt actatgttct taatttcaag aaatctttcg acgaaaaagt catccagtac
     7921 ttcatctcgg aatatttaca gggtagaaca cctaacccat gtatcgcatg taacaaatat
     7981 attaaatttg aagaactttt aaacaaagct ctagccatgg atatccccta catagccacc
     8041 ggtcactatg ctacagtcga gaacaagaaa gggaggtatc ttcttaagaa atctaaaagt
     8101 aagactaaag atcaaacata tgtattgtac aatctaacac aggaacaact ttcacatacc
     8161 gtcttccctt taggtaagta tgacaagaat aaagtaagag aattagcaaa aaaatataat
     8221 ttaccagtag ctaacaaaaa agatagtcaa gagatatgtt ttgttgagga taataattac
     8281 ggtaaattca tagaagagaa tagtgacagt gatttagtac caggggattt tgtaaataca
     8341 aaggaagaag taattggaaa acacaaaggg atataccatt acaccatagg gcagagaaaa
     8401 ggactaggaa taacttcaaa caaagctctg tatgttgtag atatagatat tgaaaaaaat
     8461 atagttatgt taggagagga gagtgattta caaaagaaag aattaatagc atatgactta
     8521 aactggatac taatagataa attagaaaag aagatgaata ttaaagctaa gataaggtat
     8581 ggagcaaagg aatctcctgc aaccatatat ccattggaga aagataaagt aaaagtaata
     8641 ttcaaaaaac cacaaagagc cataacttct ggacaagctg tagtgtttta caaaggtgat
     8701 tatgtagtag gtggtggtac tattgagtaa ttactttcca accactcacc aatcttaaat
     8761 ctccatttct ctctaagcat gtagatatta ccagatccat ttgtccagat atgttacttc
     8821 ctttctcaat ttccatttcc tgtacagttg tacattccaa tcttacatct ccaaacttca
     8881 taacagtccc tagtagtagg tctttgttgt taagatagtc gagtagttct ccaaagaggt
     8941 aatatttttt agcaagggat agcccattgt gtacatgtaa ttggtatatc ccatctttaa
     9001 gaaacattgg tcctgctaga acaatgtctc cttgtttgtt tagttgtttt ctccaactgt
     9061 aatctgtttt aaggagttca tttaaattac tgtaaacaga attagaatat tcaccaaggt
     9121 atgtatattt gaaatacata tttttcttct cccaaccact tggaagatat ctgttggata
     9181 gttcgaagga gactgtagta ggagtattta cctttaaatc tagtaattcg cctaagtcac
     9241 tactttgtat ctcctctgat gcatttgctt gtaatactgt aatgtcttgt gtatttacaa
     9301 agagagagtc aaagagagta gggtagaata ccgtaaatgt aaaaatggct gtaccagtga
     9361 atagtacagc cattattata atcgaaacct ttttattcat tacttactat tctttaagta
     9421 tgtcttagta aagaatacag aatatgtaac taacatagca actagtaata gttctaccat
     9481 atacaccact gtattatctg tagggcctgt agctgcaagt acaacaccag tatctttttt
     9541 tgtagtatca tcttggacac cttcagtatc tggatcatca tcttttggag cacagagtgg
     9601 atctatagaa gaaatagtag aatcatactg acaatcaact ggacatgcag gatcatctgc
     9661 ctctaattca ccatcccatt tacacattac tggtgcttta caactctcgt ctattgaaga
     9721 aatcgtagca tcatactgac aatcaactgg acatgcaggg tcatctgctt ctaattctcc
     9781 atcccattta cacattactg gtacacacaa ggcattattc ccaattgctt ttctgaatga
     9841 gaatcctgct atttctaaaa tccaagtatt acttgaatca gtccagggaa catctactaa
     9901 cgtatctgta tctgcaggtg cagttccatt aaattttgta tctgttccgt ataatgttaa
     9961 tacatatgga taatcataat ctgactgatt tcttactctt agtctacctt ctgtctcgga
    10021 aggtctacac atggaagtca aattgaaatc acttctgatt gcaacaacaa ctggttgaca
    10081 attattattg aaaattgctt gaccttctgt tgtccagttc tttcctgcgt aatgttgtcc
    10141 atcaccataa tcaaaaggct tgattatatc tccccaactc tccgttatac cttcaaacca
    10201 aactggacca ttatgaccat catgcccact atctccaacg atggaatcgt catccacata
    10261 ctcttttttg ggaccatatg gatttgaatg agcacttctt gcatgacaaa tctctctatt
    10321 tttttctgct tgagcctcaa ctctccctac aggagaaaaa gctaagaaca acgcaaacat
    10381 gatagaaggc agaaccacaa aagataataa tttatctttc atactatcaa taataataat
    10441 ttattatcaa tggggtttgc cattagctca gtacttttaa ggcaaccata tataaaaagt
    10501 acttcatcgc tattgatgaa tgaattatag ttaatattta ataaaaaatc aatactatag
    10561 gagtgaatct tattccaacc tatactcaac aatatctaac acagaagata taaattcttg
    10621 ggattcctca ggcatacaag aaacagaaaa aactgggaaa aattcatgat ctataccctt
    10681 tccactacta tccttacaca ccacctggat actcggtaat ccaccattat gaaccaaaag
    10741 atatttaaat ctacattctt taccatctac taacccttca ccatcctcta tgccaaattt
    10801 aaaatatttc tcatcactta tactcaatat attaaatctg gaaacctcaa tctcttgctt
    10861 aaacactttg gaaaacagtg gaagaatagc atcaacctcc tcgtgactta actccatata
    10921 ttcacaaccc gtctttttat atggcgctaa atcattatat ctcaaagtac aacactgcca
    10981 ctccttagtt acctccctta cttttgcaag ccttatatcc aatgatgagc tattgttctt
    11041 ttctttctcc atattattta atctctagaa atttagatgt tagaacctcg aatatctttt
    11101 tgtctgtgtt agataatcta tctagtttgt ctttaaaaag gcagtgggta tgggtgatag
    11161 actttttacc ttcctcttgc aatgatctga tgaaaatatt cccctctttc ttactattta
    11221 tataatggaa gaaacaggtt attcttttcg aagactcttg aacacttccc tcaccaccgt
    11281 agagaccaaa ttcaaaagac tcctctggct ctatagtggc tataagatgg aaattgtgta
    11341 atctcatatt ctcctgtaaa aatccaactg aaagaagtac atcgtttatt tcctgcgttt
    11401 ctgacatatc aggataaaaa gaaacaagca ttggatcttc aacatttttc attagcaatt
    11461 cttctatttg tttaagattc ccttctagta cttctacact agtatgttct gtttcgatgg
    11521 ttggattcat atctcttctt cttaattatt cttatttaat ctaccacacc ccatagcatt
    11581 gagtaaagta cagtaaaaga tatataatac aaaaagatct ttaacaatat atctgggtga
    11641 aatttagatg atgacttcaa gtcgtcatgt aatatcaccc atgtaatcaa actcaaaagg
    11701 agaagaaatg aaaaacgagt ataggcaata tctaataaag ctaggtgggt tttttacgtt
    11761 gttttcttta ctaacacttc tgtctcagga atggcttttt ttatggtttt tagtaaatac
    11821 ttctgcttta gctctatttc ttgttctaag agtaattgtg gaaaagcctt ttacgatttt
    11881 tgacattttc gttatgctac cacttttgct cttctttgtt actctagcac tagcattttt
    11941 tgatgtaata gatgacagtt ttgtaaagca atccttgctg ttaacaatct taacaccaat
    12001 ctttattctg ataggaggat tacttcaacc aagagagact tgattaccat gggtaaacac
    12061 aacaggtgct tattgcacaa acataggcac ctgctaatac cccctcttgc ttatactcca
    12121 catatgctac aatggtatat atcatgacac agacatcaaa actctttaca ttcatccttc
    12181 tgttcatatc ttccttctta attgcatcat actttctctt ttctaaatat ctagattcag
    12241 atgtaaaaca agaacgagaa gaaataatac aactaaaaaa ttctgaaaca ttactctcac
    12301 aaatgaccct tgaagaaaaa gtaggacaaa tgttcctaat gggattctgg ggtacagaac
    12361 cagattacta cataaccaag atgattaaag agcgtcacat tggtggagta atactcttta
    12421 agtacaatgt aaaagacaga gaacaattaa cacaactttc aaaaaatctt cagttactta
    12481 gtgacaaagt ccctctattc gtatccatag atcaagaggg tgggatagtc tccagattag
    12541 gaacagatat tgtctctgaa attactgcac aaagtgaaat taaaacagaa aaagatgcat
    12601 acaatatatc taa
//
LOCUS       DAOQWP010000019        11210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_6246_length_11210_cov_5.517974, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000019 DAOQWP010000000
VERSION     DAOQWP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_11210_cov_5.517974"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKI16_02850"
     CDS             complement(<1..600)
                     /locus_tag="PKI16_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53387.1"
                     /translation="MDTDYSQKKLNKRSLFFLIFLIGFSMLVLKITKTNDIQFFKGSS
                     ESVICSNEVIDQGYDNNLVFLDMSEDGALTDVTPKTKGSVLGTTCGNALNKYTGTEAC
                     TIPGTSDNVSIKGWVNSEANVEIVSITVPIRLLSGIYSVKDSNRNLTYENPVYKPAGE
                     LFNKKQILIRSTPGEGNKEMKENIIDASVTKKAYSTKYTV"
     gene            complement(624..2198)
                     /locus_tag="PKI16_02855"
     CDS             complement(624..2198)
                     /locus_tag="PKI16_02855"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNR53388.1"
                     /translation="MKIARLPSKRKNNVILWILVIGLPILVYASYQAVQLITKASGDT
                     QPKNLVISNLSTSSATISWVTEASVKGSVIPILGGNEKSPVVDKRGNENRNTHYVELT
                     SLEPNTKYDFKIVSGSDEYASLDNVTMSFKTAPITAGTPTPNPIHGTVSGSSGDDVIV
                     YAMLKDKSVYPVSAVIPSGGNWITDLSAFRKISDYSLVLTENTTNLVLVSVGGPDKGA
                     VLQGTYSSLFDSNGKLKSTNDFTIGSNSNLYAYFAAKSMLMAESTVPTPSIEQPEEES
                     EEEPTKESKFKIVMDLEWVDMVSGSSVTAPTGVSSVQVTNLTDTGFTVLWVSAQKEAG
                     SISYGTSSSSLSSEALDERDGATNRGTYYVHSVSVTMVQPETKYYFKVKSGGAEYSNT
                     FDVTTYATLSSPPPFESITGEVKNIPSTEELVVVGYITDKDGSGTTGSSNKISVLADD
                     NGKWILSIADTRNTSGSSYFEYTSADEITFSLVSTVNSGSLIKTISSAKNTDVEITAG
                     SGSTGNVDILDNYGVL"
     gene            complement(2236..2565)
                     /locus_tag="PKI16_02860"
     CDS             complement(2236..2565)
                     /locus_tag="PKI16_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53389.1"
                     /translation="MNEIPPIYWMIVIGAIALMICVVLFYIAMVLLESKRAIEDSRKI
                     IEDAGKTLRQVDLIVNDIQTSVSSVKESVDVINQSILVPIRRLGYNVSTITGFLGGIF
                     GRKKEEE"
     gene            complement(2585..5476)
                     /locus_tag="PKI16_02865"
     CDS             complement(2585..5476)
                     /locus_tag="PKI16_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNR53390.1"
                     /translation="MSITTVKVLRTISKIILLIVGIGIIGYGGLMVYKSVVNKPQRVR
                     ITNVTDSATTITWVTDNPVRGIVYYKDEGTFLPGPLGFLGSKVAYDDRDFARAQDECV
                     KAFNENAKNTKDTNFTVDGSGFDCENIPVEKLGAYYTHSVTIKNLNESSTYFFVVGNG
                     IWSWSVDGVDKNIGENDLVVANSFNFKTAKLLEEVSTPNIAYGTVYAGVKDPGLEAFY
                     EDPSKDSLVFGYAILNGADSQLVSAVTNASGGWILDKSNLRDAEGNLISDLTGVTLEL
                     CAQYENVSPKGCVEVTEDLEKDTVVDLQGNFVEDLTIYEKDMLLKKFGELLNKVYAMS
                     SAGHDMDRCTDSGVSGCSTDAKCIEKCPVTGTDLFDWVDVGTVSAGDCELPSFCGDCT
                     EQDAANGCNRGTLGDGKSKKCIKESGGYFWTNYSTDDCSVGGGGNEGIPCEDPETGNL
                     VQDGKKCSNGVVCKKGDCNDEDGVQGPDPTPTIKVTCKDKYGATINEGKPCPISGGTC
                     KSNGKCESSGGGGDGTEEEASNTEYCTVEPLYSSYGDLVNCIVSCEDGKEIYMNKGQC
                     SSFSNDIPANGNPGYGEGVKIVASKISLPKLNLTQLKNLVEKSDDVKNTVIKNAEKLN
                     KEENKTGKTWRNFEKYCQALGGTPTLETGTQKYKCLINNEEIIGLSLIGKAYAQNSSS
                     DGYTIYLPEYGMYSFQLGDYQLTTDTTGGNTYYIFYIEANDKKGFQMPADLDNPTEKE
                     DIVLSSNAMQIQHKKVASAQAYTLNEGINLISFNFIPVSTELGPLTAKGVIDEATKKG
                     VTIQYISSFDGGRWAKGYTCKNDKCSGNDFNILPGKGYLVYATKGGEMTIPGYNLKSS
                     IPVNLSGGWNLVGIHGYTTAYTARTLIDSMNKIDRLTANNVTWWPTSKGMYEGLQVTE
                     EQQYGFDFPISPTNGYFVRISSFQPEDSKCKSLLWNEGGTLNGACGVTK"
     gene            5556..6284
                     /locus_tag="PKI16_02870"
     CDS             5556..6284
                     /locus_tag="PKI16_02870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNR53391.1"
                     /translation="MKKYFIKHKLAIGDITHLSDSDSELILSGKEAHIEDIVEIETYE
                     KIFLGQITDISKNSVEVEIIEEKGTRDTKYVPSITIIQSLSNDSKFNYFIEKSVEIGI
                     EKIIPVETKYSLKNKKKALKDYPLWNKIVKDATEQSRNIFPTIIEKPLNMEELAKYQF
                     ENNSIKICLATENVPTKYLSDIVIDSRPVYIAIGPEKGWSSSDIDILKRLGFQFIQLK
                     GNILRTETTGLVIGSIIKYIKGEI"
     gene            6284..7276
                     /locus_tag="PKI16_02875"
     CDS             6284..7276
                     /locus_tag="PKI16_02875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HNR53392.1"
                     /translation="MKKPTRTNYRKPRNFVQQNNKDRFFTKEDIFISRIASILLVPKD
                     KVESLFSQRAITTIRLNPLKGNTEKTKLSLEEKDIELQEIPSVKDTYFVLNMDKSEMS
                     QTEEYQDGKFYIQNLSSILAALVLDPKPGEKVLDMCAAPGSKTTHISALMNNKGMIIA
                     NDSDPARVSSLRNVLTQFGAKNAKATLSDATDFGTKYPNYFDKILLDAPCSGEGMIYM
                     RGEKPLRFWSIDKIKRCSYIQRDLILSAFRALKPGGTMVYSTCTLEPEENEGVITFLL
                     DHFPNAQVEETGLHYDKGITKWSGNVYNPKVRKTTRLLPSSKMMAFYIAKIKKI"
     gene            complement(7478..9232)
                     /locus_tag="PKI16_02880"
     CDS             complement(7478..9232)
                     /locus_tag="PKI16_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53393.1"
                     /translation="MIKRFKEWVSSHKRLTILFGILFILVVFFVIFLLFGQKYIGSPI
                     GKINLPNIENLFSKDDEEIPAEEDSPSEEIDTIKDLELDFLGSPVLVSKQFNLSKPGE
                     DGKDIDDEIKGVYKTADVLRGTYKGESLKDCEVFILKYPSLLMEARIISCDNKKIYVI
                     GTYAFEYGSPIDVPEENVVQVLSDSENDLNSFFNNQSLPEFGKTIVSDSGNSFEVRSL
                     TFEVYEKGELTEIDKIDGLPVYNIASGGKGESLIASPEGFYQILVVSPGIVSINGEYN
                     EPQVTIKLNEGGTFSAIYDYISIGGCFNNYIEVADVKLASLTKIGVGINGDSIYQDTD
                     RNAAYLKKMYNEDYAIKDGVHKWNNLSDDDTTPYAYDKYLENYPVIYWVDPFDRIIKF
                     ANRNFISTGGCAKPAIYLYSEKAIDLNVKVIPNGHLTFTSPKYPVNGWNVTASVNGSV
                     QYQNEIYPYLWWESTSYGYVTPENGWVIKKETAEEKIGGILSDYGLNQTEINDFLTYW
                     IPKIERENSEYIFVTFLVNDQVNQIAKLKFSIEPDNSLRLFMIYKPLDEYEKVTPLKT
                     EKLLRNGLTVIEWGGAKY"
     gene            complement(9301..>11210)
                     /gene="pnp"
                     /locus_tag="PKI16_02885"
     CDS             complement(9301..>11210)
                     /gene="pnp"
                     /locus_tag="PKI16_02885"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNR53394.1"
                     /translation="EKFYASGKINGSRFVKRERFPSDDAVLKARMIDRAVRPRFPKDY
                     KNEINLIITVMSYDEENDPLLLALNASSVALMNSTAPFNGPIASVRVSEDGKHIFKAM
                     DITSQEDKMNYVVSGDGKVFTMIDAACYEVEEEKILESMKKSLDLMNPWLKAQEEFLS
                     KLEKKNKEYPSYALPEEAVTIVKEFLTEEKIRENLIKADDKLHESTKEELSQNFESKY
                     TKAVLAEAYDYLLKKALRKIVLEENKRVDGRGIEEIREMYTEVDLLPRVHGSALFTRG
                     VTQTLTIATLGSLRDVRLVDDMEGEREQRYMHFYNDLPFAYGQCDRVKLMPSRRALGH
                     GMLAEKALLPVIPTEKEFPYTILVMSEIQGENGSSSMASACGSSMALMAAGVPLRKPV
                     GGIACGLVTAEDGSFKVLTDMQGVEDFYGDMDFKITGTKDGITAVQMDTKTDGLSMEI
                     VEQTFKQSKEARMKVLEKMAETISEARKELSSFAPRVAQVKVSVDKIGEIIGPGGKHI
                     KELSETTGAEVEIEEDGTVNIYATSQEAIDMAVKRIEMYDFKPVVGQIYEGKVASIMP
                     YGGFVDLVPGIAGLLHVSEISDDFVKDVRQFINEGDIIKVKVVGIDNMGKIKLSMKGL
                     DKKDPKEQVKE"
BASE COUNT         3395 a         2334 c         1727 g         3754 t
ORIGIN      
        1 aacggtatat tttgtactat atgccttctt tgtaacagag gcatcaatta tattttcctt
       61 catttcctta ttaccttccc caggagtact cctaataagt atttgcttct tattgaatag
      121 ctcacctgca ggcttatata caggattctc gtatgtaaga tttctattgg aatctttgac
      181 agagtatatt ccagaaagaa gtcttatagg gactgtaatt gagacaattt caacatttgc
      241 ttctgagttt acccagccct taattgagac attatcgctt gttccaggaa tagtgcatgc
      301 ttccgtccca gtatatttgt ttaatgcatt tccacaagtt gttccaagga cacttccttt
      361 agtctttggt gtaacatcag ttagcgctcc atcttcagac atatctaaaa acacaagatt
      421 attatcataa ccttgatcta taacttcatt agagcatatt acagattcac tacttccttt
      481 gaaaaattgt atatcatttg tttttgttat tttcagaaca agcattgaga accctattag
      541 gaatataagg aagaagagac ttcttttatt caattttttt tggctatagt ctgtatccat
      601 ataactcccg attatatcag aaattaaagt actccatagt tatccaagat atctacatta
      661 cccgtgcttc cactacctgc agttatctct acatcggtat tttttgcact tgaaattgtc
      721 ttaattaggc taccagaatt taccgtagat actagggaaa atgtaatctc atctgcagag
      781 gtatattcga aataggaaga accacttgta tttctagtat ctgcaattga gagtatccac
      841 ttaccattgt catctgcaag aacagatatc ttgttactac ttccagtagt accactgcca
      901 tccttgtcag taatatatcc aacaacgacc aattcttcag tagagggaat gttctttact
      961 tcaccagtta tagattcaaa tggtggagga gagcttaagg tggcatatgt tgttacatca
     1021 aaagtattgc tgtattccgc acctccagat ttaactttga agtagtattt ggtctctggc
     1081 tgtaccatcg ttacagacac actatgaaca tagtaggtac cacggttagt tgctccatct
     1141 ctttcatcta gcgcttcgct agagagagat gaggagcttg taccatagct aatagagcct
     1201 gcttcctttt gggcagagac ccaaaggact gtaaatccag tatctgtaag atttgtaact
     1261 tgcacactag atacaccagt aggagctgtt acactgcttc cagagaccat gtccacccac
     1321 tctaaatcca ttacaatttt gaatttagat tctttcgttg gttcttcttc agattcctct
     1381 tctggctgtt ctattgaagg tgttggaact gtactctctg ccatgagcat ggattttgct
     1441 gcaaagtatg catacaaatt ggaattactt ccaattgtaa aatcgtttgt actttttaat
     1501 ttaccattgc tgtcaaaaag agatgaatat gttccttgta atacagctcc tttatctggt
     1561 cctccaacag atacaagcac aagattggtg gtgttttctg ttagtacaag agagtagtca
     1621 gagatttttc gaaatgcaga aagatctgtt atccagttac caccactagg tatgactgca
     1681 gatactggat atacagattt gtcctttaac attgcatata ctatgacatc atcaccactg
     1741 ctaccagaga cagttccatg tatagggtta ggcgtaggag tcccagctgt aattggggct
     1801 gttttaaatg acattgttac attgtccaga gatgcatatt catcacttcc tgaaactatc
     1861 ttgaagtcgt atttggtatt tggttctaag cttgttagtt ctacatagtg ggtgtttctg
     1921 ttttcatttc ctcttttatc tacaacagga cttttttcat ttccaccaag aattgggatt
     1981 acagatcctt ttacagatgc ttctgttacc caggatattg ttgcagagct tgttgaaagg
     2041 ttactgatga caaggttttt tggttgtgta tctccgcttg cttttgtgat taattgaaca
     2101 gcttggtatg aggcatacac aagtataggc aaaccaatca caagaatcca aaggattaca
     2161 ttattctttc ttttagatgg taatcgggca atcttcatta cctaatatat tacctgtatt
     2221 tgtggataat gacaattatt cttcctcttt ttttcttcca aatatacctc ccaaaaagcc
     2281 tgtgatagta gagacattat agccaagcct cctgatagga acaaggatag attgattaat
     2341 cacatcaact gattctttta cagaggatac acttgtttga atatcattta ctattaaatc
     2401 tacctgtctt aatgttttac ctgcatcttc tattattttc ctactgtcct ctatagctct
     2461 cttactttca agaagaacca tagctatgta gaagagaaca acacagatca taagggcaat
     2521 agctcctatg actatcatcc agtatattgg tggtatttca ttcatggcag tatttatttt
     2581 taaattattt agttactcca caggctccat ttaatgtgcc tccctcattc caaagtagag
     2641 atttacattt actatcttct ggttgaaatg aagatattct tacaaagtaa ccgttagtag
     2701 gactaattgg gaagtcaaaa ccgtattgtt gttcttccgt tacttgtaat ccttcataca
     2761 tacctttgga tgtaggccac catgttacat tgtttgcagt taatctgtca atcttgttca
     2821 tggagtctat tagtgttctt gctgtatatg cagttgtgta accatgtatt cctactaaat
     2881 tccatcctcc cgataggtta actgggattg aggatttaag attatatccg ggaattgtca
     2941 tttctccacc tttggttgca tatactaggt atcctttgcc tggaagtata ttaaagtcat
     3001 ttccactaca tttgtcattt ttacatgtat atccctttgc ccatcttcca ccatcaaagg
     3061 aagatatgta ctgtatagtt acacctttct tagtagcttc atctataacc cctttggccg
     3121 taagtggccc taattctgta gataccggta tgaagttaaa ggatattaga ttaatacctt
     3181 catttagagt gtatgcctgt gcagaagcga cttttttatg ttgaatctgc attgcattag
     3241 aactaagaac aatatcttct ttttctgtag gattgtctag atctgcaggc atttggaagc
     3301 cttttttatc gttagcttct atatagaaga tgtaatatgt atttccacct gtagtatctg
     3361 ttgttaattg gtaatctcct aactggaagc tatacatacc atattctggt agatatattg
     3421 tatatccatc agaggaagaa ttttgagcat aggcctttcc aattaaacta agccctatta
     3481 tttcctcgtt attaatcaaa catttatatt tctgagtccc agtctctagg gttggagtac
     3541 cacctaaagc ttgacaatat ttctcaaagt ttctccatgt tttaccggtc ttgttttcct
     3601 ctttgttcag tttttctgca tttttaatta ctgtgttctt cacatcatct gacttttcta
     3661 ccaaattttt taactgtgtc agatttaatt tcggtaaact aatcttagaa gcaacaattt
     3721 ttactccttc accatagcca ggattaccgt tagctgggat atcgtttgaa aaagaagagc
     3781 attgaccctt attcatataa atctccttac catcctcgca cgaaactata caatttacta
     3841 aatcaccata gctactatac aatggttcaa cggtgcagta ctccgtattc gaagcttctt
     3901 cctcagtacc atcacctcct cctcctgaac tctcacattt tccatttgat ttacatgttc
     3961 ctccggatat tgggcatggc tttccttcgt ttatcgttgc cccatattta tctttgcaag
     4021 ttactttaat cgtaggtgtt gggtctggcc cttgtacccc atcctcatca ttacaatctc
     4081 cttttttaca aactacacca tttgagcatt tttttccatc ttggacaaga ttgcccgtct
     4141 ccggatcttc gcaagggatc ccctcgtttc caccaccacc tacactacaa tcatcagtcg
     4201 aataattagt ccaaaaatat cctccactct cctttataca tttttttgat ttaccatctc
     4261 ccaacgttcc acgattgcat ccatttgcag catcttgttc agtacaatca ccacaaaagg
     4321 aaggaagctc acaatcccca gcactcaccg taccaacatc aacccaatcg aacaaatcag
     4381 tacctgtaac tggacatttt tcaatacact ttgcatcagt agagcaacca cttactcccg
     4441 aatcggtgca tctatccata tcgtgacctg cagagctcat tgcatagact ttattaagta
     4501 attcaccaaa tttctttaaa agcatatcct tttcatatat ggtcaaatcc tcaacaaaat
     4561 tcccttgtaa atctacaact gtatcttttt ctaaatcctc cgtaacctct acacaaccct
     4621 ttggacttac attctcatat tgcgcacaaa gttctaaggt tacacccgtt aaatccgata
     4681 tcaaattacc ctctgcatcc ctgagattac tcttgtccaa tatccatcct cctgaagcat
     4741 ttgttacagc agatactagc tgtgaatccg ctccatttaa aatggcgtaa ccaaatacta
     4801 aagagtcctt agagggatcc tcgtaaaaag cttcaagccc aggatccttt acccctgcat
     4861 atacagtccc ataagcaata ttaggagtag aaacctcttc tagtaattta gctgttttaa
     4921 aattaaaact atttgcaaca accaaatcat tctcaccaat atttttatct acaccatcta
     4981 cactccaact ccatatacca ttaccaacta caaagaaata tgtactactt tcattcaaat
     5041 tcttaatagt tacactatgt gtgtaatatg cacctagttt ctctactggt atattctcac
     5101 aatcaaaacc actaccatct acagtaaagt tagtgtcctt agtgttctta gcattctcgt
     5161 tgaaagcctt tacacactca tcctgtgctc ttgcaaaatc tctgtcatca taagcaacct
     5221 tactgcctag aaaccccaaa ggtccaggaa gaaatgtccc ttcatccttg taatacacaa
     5281 ttcctcttac tggattgtct gtaacccaag ttattgtagt tgcagagtcc gtcacattcg
     5341 ttatccttac tctctgaggt ttgttaacaa cggacttgta taccattaaa ccaccataac
     5401 caataatacc tataccaaca attaaaagaa taattttaga tattgttcgc agaaccttaa
     5461 cagtggtgat actcataatg aacagattta aatatatata catatatcaa acaaaataat
     5521 ggggaatatg tcaaatatgg tgatataatc caatgatgaa aaaatatttc ataaaacaca
     5581 aattagcaat tggagatata acccacctgt cggattcaga tagtgagcta atcctctccg
     5641 gcaaggaagc acacatcgag gatatcgtag agatagaaac atatgagaaa atcttccttg
     5701 gacaaatcac agatatatcc aaaaactccg tagaggtaga aattatcgaa gaaaaaggta
     5761 ctagagatac gaaatatgtg ccaagcatta ccatcataca atccttaagt aatgattcga
     5821 aatttaatta cttcatagaa aaaagtgtag agattggtat agagaaaata atcccagtag
     5881 agactaaata ttctcttaag aacaaaaaga aagcattaaa agactatcct ctctggaaca
     5941 aaatagtaaa agatgctaca gaacaatcta gaaatatctt ccctacaatc attgaaaaac
     6001 cattgaatat ggaagaatta gctaaatacc aatttgagaa taattctata aagatatgtc
     6061 tagctacaga aaatgtccca acaaaatacc ttagtgacat tgtaattgat agtagaccag
     6121 tatatattgc cattggtccc gagaaaggat ggagttcttc agatattgat atacttaaaa
     6181 gacttggttt ccaatttatc cagcttaaag gaaatatcct aagaacagag actactggat
     6241 tggtaattgg aagcataatt aaatatatta agggagagat ctaatgaaaa aaccaacaag
     6301 aacaaattac agaaaaccaa gaaattttgt tcaacaaaat aacaaagatc gtttctttac
     6361 caaggaagat attttcatat ctagaatagc cagtattttg ctagtaccaa aagataaagt
     6421 ggagagtctc ttctcccaaa gagccataac tacaattaga ttaaaccctt tgaaaggcaa
     6481 tacagagaaa acgaaactat ctcttgaaga gaaagatatt gaattacaag aaatcccttc
     6541 tgtaaaagac acatactttg ttttgaatat ggataaatcc gagatgtcgc agactgagga
     6601 ataccaagat gggaaatttt acatacagaa tctttcaagt atattagcag ctttagttct
     6661 tgaccctaaa cctggtgaga aggttttaga tatgtgtgca gcaccaggga gtaaaacaac
     6721 tcatatttct gcattgatga ataataaagg tatgataatt gcaaatgatt cagatcctgc
     6781 aagggtatct tctttgagaa atgtgcttac acagtttggt gctaagaatg caaaagctac
     6841 actgagtgat gctacagact ttgggacaaa atatcccaac tacttcgaca aaatactttt
     6901 ggatgcgcca tgcagtggag agggaatgat atatatgcga ggagagaaac ctcttaggtt
     6961 ctggagtata gataagataa agagatgttc atacatacaa agagatttaa tactttctgc
     7021 atttagagca ttgaagcctg gtggtacaat ggtttattct acatgtacat tagaaccaga
     7081 ggaaaacgaa ggagttatta cattcctact tgaccatttc ccaaatgcac aggttgaaga
     7141 gactggctta cactatgaca aaggtataac caaatggagt gggaatgttt acaaccccaa
     7201 ggtaagaaag accaccagat tactaccaag tagtaagatg atggccttct atatcgctaa
     7261 gataaaaaag atttagctca aaggactgtc ccagctctgc ccccaaatcc taagcaaaaa
     7321 acaccttctc tagctgaaaa cgccaacaac acaaacccaa atcgcttagc gagtagttgg
     7381 gttcgaaagg tgaagactcc gaacaaagct gtatctaggc aaggctcgct tctattagat
     7441 aagggactgt cctgggacag tcccaagaat tctcgtacta gtacttcgcc cctccccatt
     7501 caattactgt taagccattt ctcaaaagtt tctcagtttt caaaggtgta actttttcgt
     7561 attcatcaag tggtttgtaa atcataaata gtcttagtga gttatccggc tctatgctga
     7621 atttaagttt agcaatttga ttgacttgat cattaacaag gaaagtaaca aaaatatatt
     7681 cactattctc tctttctatt ttgggaatcc aatatgttag gaaatcatta atctctgttt
     7741 ggttcaaacc ataatcagaa aggatcccac caatcttttc ttcagcagtt tctttcttta
     7801 tcacccaccc attctctggc gtaacatatc cataagaggt tgactcccac cataaatatg
     7861 gatatatctc gttctgatac tgaacagatc catttactga tgcagtaaca ttccaaccgt
     7921 ttacaggata tttgggggat gtaaatgtta aatgaccatt gggaatcact ttaacattaa
     7981 ggtctattgc tttctcagaa tagagataaa tagcaggttt agcacaacct ccagtagaga
     8041 taaagtttct attagcgaat tttataatcc tatcaaaagg atctacccaa tatattactg
     8101 gataattttc taaatattta tcatacgcgt atggagtcgt atcatcgtca gaaagattat
     8161 tccacttatg taccccatcc tttattgcat aatcttcatt atacattttc ttcaaatatg
     8221 cagcatttct atcagtatct tgatagatgc tgtccccatt tataccaacc ccaatttttg
     8281 ttaggcttgc tagttttaca tctgcaactt ctatatagtt attaaaacaa cctccaatag
     8341 aaatgtaatc atagatagca ctgaaagttc ctccttcatt caactttatt gtgacttgtg
     8401 gttcattgta ttcaccattg atggatacaa tacctggact tacaacaagt atttgataaa
     8461 atccttctgg agaagctatt aaagattctc cttttccacc tgaggctata ttatatacag
     8521 gcaatccatc aattttatct atctctgtta actcaccttt ctcgtatact tcgaaggtca
     8581 gactccttac ttcgaagcta ttaccgctat cgctaacaat cgttttccca aattcaggta
     8641 gagattgatt attgaaaaag ctgtttaaat cattctctga atctgagagt acttgaacaa
     8701 cattctcctc tgggacatca attggggaac cgtattcgaa tgcatatgta ccaataacat
     8761 atatcttttt gttatcacag ctgatgattc tagcttccat caacaaagat ggatatttta
     8821 agataaatac ttcgcaatcc tttagactct ctcctttgta agtacctctc aaaacatcgg
     8881 cagttttgta aactccttta atctcatcat caatatcttt tccatcttct cctggtttag
     8941 acaaattgaa ttgcttgcta acaagtacgg gagatcctag gaaatctaat tctaaatcct
     9001 ttattgtatc aatctcctcg gaaggtgaat cctcttcagc aggtatctct tcatcatcct
     9061 tgctaaataa attctctatg tttgggagat taatctttcc tataggggaa ccaatatact
     9121 tctgaccaaa gagaagaaat atgacaaaaa aaacaacaag gataaataat atcccaaaaa
     9181 ggattgttaa tcttttatgg ctagagaccc attctttgaa acgcttaatc ataaggaaat
     9241 agatttaact tagcttaaac taagttaaat ctaacacttc ccaaggtaat cttcaacagg
     9301 ttactctttt acttgttctt tgggatcctt cttatctaga cctttcatag aaagttttat
     9361 ctttcccata ttgtctattc caacaacctt taccttgata atatctcctt cgtttatgaa
     9421 ctgtcttaca tcttttacaa aatcatctga gatttcagaa acatgaagta atccagcaat
     9481 acctggtact aaatctacaa atccgccata tggcattatg gatgcaacct tcccctcata
     9541 tatttgtcct acgactggtt taaagtcata catctcaatt cttttaactg ccatatctat
     9601 agcttcctgt gatgtagcat agatatttac tgtaccatcc tcttcaatct caacctctgc
     9661 accagttgtt tctgaaagct ccttaatatg ctttcctcct gggccaatta tttctccaat
     9721 tttgtctaca gaaaccttaa cctgagcaac tcttggcgca aagctagaaa gctcttttct
     9781 agcttcagaa attgtttctg ccatcttctc aagaactttc attcttgcct cttttgactg
     9841 tttgaatgtc tgctctacta tctccatact tagaccatct gtctttgtat ccatctgtac
     9901 tgctgtaatt ccatcctttg ttcctgtaat tttgaaatcc atatctccat agaaatcttc
     9961 tacaccttgc atatctgtaa ggaccttgaa ggaaccatct tctgcagtaa ctaaaccaca
    10021 agcaattcct ccgactggtt ttctaagggg tacacctgca gccattaagg ccattgaaga
    10081 accacatgca gatgccattg agctagaacc attctctccc tgaatctcac tcataaccaa
    10141 aatggtatat ggaaattctt tttctgtagg gataactgga agtaatgctt tctcagctaa
    10201 cattccatgt cctagagctc ttctacttgg cattaatttg actctgtcac actgtccgta
    10261 tgcaaatggt aaatcattgt agaaatgcat atatctctgc tccctctcac cttccatgtc
    10321 atcaaccagt cttacatctc ttaaactacc taaagtagca attgtaagag tttgagttac
    10381 tcctctagtg aacaatgcag atccgtgtac tcttggaagt aaatctactt ctgtgtacat
    10441 ttctctaatt tcttctattc ctctaccatc aactctcttg ttctcttcta atacaatctt
    10501 tctcaatgct ttcttaagta ggtaatcata agcttctgcc aatacagcct ttgtatattt
    10561 actctcaaaa ttctgggata attcttcttt tgtactctca tgtaatttgt catctgcttt
    10621 aattagattc tcccttatct tttcctctgt taaaaactct ttgactattg tgactgcttc
    10681 ctctggaagt gcatatgatg gatactcttt gtttttcttc tctaattttg aaaggaactc
    10741 ttcctgtgct ttcaaccatg gattcatcaa gtctaaagat ttcttcatac tctcaagtat
    10801 tttttcttct tcaacttcat agcaagctgc atctatcatt gtgaatactt ttccatctcc
    10861 ggatacaaca tagttcattt tgtcttcctg tgaggtaatg tccattgcct taaatatgtg
    10921 ctttccatct tcgcttactc ttacactagc tattggccca ttgaaaggag ctgttgaatt
    10981 catcaaagct actgaagatg catttaatgc taaaagaaga ggatcattct cttcatcata
    11041 tgacataaca gtaattatga ggtttatttc atttttgtaa tctttaggga atcttggtct
    11101 aactgcccta tctatcattc ttgctttaag tacagcatca tcacttggga atctttctct
    11161 ttttacaaat ctagatccat ttatctttcc tgatgcatag aatttctcta
//
LOCUS       DAOQWP010000020        11000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_6391_length_11000_cov_6.014344, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000020 DAOQWP010000000
VERSION     DAOQWP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6391_length_11000_cov_6.014344"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PKI16_02890"
     CDS             <1..716
                     /locus_tag="PKI16_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNR53395.1"
                     /translation="ITAQSEIKTEKDAYNISKKRAKELKDLGITMNFSPVLDNIQKED
                     SFLYDRVFRTDISLLGNKMISGYTDGGVIAVPKHFPGHPDNSIDSHKTLPEVNTPEAD
                     FDTYASQFKEVLSNPDIQAIMVGHILFKQIDANNPSSLSSKIITEILRNKYGFKGVVI
                     TDDMQMGALTESLTIAQAAVKAVTAGNDLLIYSGEPEQQAEAYNAILDAVKKGKISEQ
                     RINESVLRILTLKANIFNL"
     gene            728..1117
                     /locus_tag="PKI16_02895"
     CDS             728..1117
                     /locus_tag="PKI16_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53396.1"
                     /translation="MQKKLAILSFLILLFLLVLTGVWALFIRKDDTSNDWKTLTEDNI
                     TLTYQYAGVNVWKYNVKGQLPNPCHSAQVDALVAESYPEQVTVKVNIEENKSTDTVCA
                     QVIQELNISGEFNASEKATVTLQVNKQ"
     gene            1141..2520
                     /locus_tag="PKI16_02900"
     CDS             1141..2520
                     /locus_tag="PKI16_02900"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HNR53397.1"
                     /translation="MLTKSDYITYLDSPLHLWALKNNQYERKLSDFDKHLIKQGYEVE
                     TLAREYVKKYISKDAQYQKIYQTDDLFARADIVVGHDIYEVKSSTEMSKEDEHDISFQ
                     YYLAKTLGKVENIYLLHLNKDYIRGKKLDLKELFVVEDVTPLAKKNWSYTEATVAEAM
                     LITNKEIPQGLSPCYDPKSCPSKALCFPDTLPEYSIYDVSRISKKNIDALREMGVVDM
                     RNIPSEFNIAQKQKLQIEATKKDKIVIDEQKILSELDSLEFPIYFLDYETYSWAVPQF
                     EGHKPYENVVFQFSLHLLKKKDGNLSHYEYIAITKKDPILDVLNILQDVIGDNGSILV
                     WNKTFEKGCNEEMARIYPEYEQFLLNLNRRIYDLGDIFKYQMYVDPKFKGSWSIKNVL
                     PVLAPDLNYHDMEIGKGDEAMVNWYRLSYENQSQEDTDSTRKNLLKYCELDTYAMYRI
                     YDFLVSKVF"
     gene            2568..2825
                     /locus_tag="PKI16_02905"
     CDS             2568..2825
                     /locus_tag="PKI16_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53398.1"
                     /translation="MESELLFGKFKTKEEAIEWALEILTLETPFCLEILEEQVEKAKK
                     ILLTYLPDPWLGVEYKGKIFLKEKYNPFVLSPAQAVGIINK"
     gene            2940..3677
                     /locus_tag="PKI16_02910"
     CDS             2940..3677
                     /locus_tag="PKI16_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HNR53399.1"
                     /translation="MNKELLIKLSVTVSVIAMFVVNALANILPINGMTTGALSDAIPI
                     YFVPAGYVFSIWGLIYAGLVVYMISMFTKFTKEDEAIYPWVIISSLANTAWIFLWHYR
                     LIYVSVIAMVIILASLIAIALILNKSKKVSIFKTLPFNIYLGWISVASIANIAAALYL
                     ANWNGFGISPEVWSAIMIVIATGLAVYSILKKRYAYSLVILWAVIGILVKFSGLSNSI
                     EVASTLGILAISSVIGFKLYFGTKTTK"
     gene            complement(3667..4047)
                     /locus_tag="PKI16_02915"
     CDS             complement(3667..4047)
                     /locus_tag="PKI16_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNR53400.1"
                     /translation="MEINEIYRCPICGNIVQLTHVGGGTLVCCGKPMELLEEKSAEEG
                     MEKHLPVATFEDGMLKVNVGSVDHPMIEEHYIEWIECIVDENVSRKYLLPNDKPYAEF
                     SVKREKDLKVRAYCNVHGLWAVTL"
     gene            4108..5082
                     /locus_tag="PKI16_02920"
     CDS             4108..5082
                     /locus_tag="PKI16_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNR53401.1"
                     /translation="MTTAIPVFIGILFCLLILFFSGKSLVTISINLARKLGVSDIFIG
                     TTLIAFTTGLPTLVVSIIAFSSNQPEIAIGNLIGTNYVNIGLALGIPAFLTTILVKKE
                     VFEKEIPLYFSLMALFTSFILDMRISTLEGILLIVFLFAAWGIIIQYGKSHKVPQQDL
                     ELNPSKEKINKRDYLSLLLLFLAILVASFGISFLTPLLAQHTNISSYLLGLTLIGVGT
                     SIPTITAGITAAKQGNTDIIVGNVFGGNILNICLGIGLLAMIKPMQMDSNAVNDLLFV
                     NIYGAIIVILILAEMKFLGKNKTLSQISGLIMVLTYLGYLAISIAKYF"
     gene            5128..7014
                     /locus_tag="PKI16_02925"
     CDS             5128..7014
                     /locus_tag="PKI16_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNR53402.1"
                     /translation="MEYSPVNKENIRNVAIIAHVDHGKTTLVDAFMKQTHLFRDNEEE
                     MGQTRILDSSDLEKEKGITISAKNISIRYKGYKINIIDTPGHADFGGEVERTLNMADS
                     CLLLVDAKEGPMPQTKFVLKKALQLGLKPILVINKIDKKLANNKRTLSKVQDLFLALA
                     SDVEQLEFPVYYAISREGKIFKEIPEGDLTIPNSTTGNVSPILDAIIETPEPTGELDA
                     PFQMQINSLEYDQHLGRYLIGKIRRGKVSINDPIILLTKEDKKQGRVKELFVKEGLAW
                     IPAKSAAVGEIVALTGLESTAIGATICALDHPEPLPEITITPPSVRIKVEPNTSPFLG
                     KEGKFVTAKQLGQRLEQEKNLNISLDIQSHGSNSYFIAGRGELQLAILVEQLRREGFE
                     LQLSKPEVILQKIDNVVSEPLEELIIDAPEIYMSTITQEVSDRKGELVNIETEEGNTR
                     FTYKILTRNIIGLHRVLMNATKGESIINSFITEYIPYTQQESLFRRGVIISSETGTAL
                     AYSLTTVQERGRLFIEGATEVYEGMIIGINNHENDIEVNPCKARHKTNVRMSHAEVTE
                     ISLKAKLPLTLEFALSFINDDELIEVTPKSIRLRKKLLTDTERVWAKRKNLTAYAKQQ
                     MGIE"
     gene            complement(7091..7393)
                     /locus_tag="PKI16_02930"
     CDS             complement(7091..7393)
                     /locus_tag="PKI16_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNR53403.1"
                     /translation="MGNKLFIGNLSWNVDDEMLRETFSAIGTVEEAVVIKDRIKNRSK
                     GFGFVKMSSEEEAQKAIAELNGKELDGRALNVSEAREENRERRSFDGGNFNRDDRN"
     gene            7520..7951
                     /locus_tag="PKI16_02935"
     CDS             7520..7951
                     /locus_tag="PKI16_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HNR53404.1"
                     /translation="MRLWSISFKYLDRMGLLAVWREALLAKKVLEDKTTGYRNHSQLV
                     RFKNTNNPVKYINAYLEDIYKEAQARGYSFSKEKFSEEIIDKHIPVNSRQVEYEFTHL
                     KNKLKRRDPVKFKELLDITHIKVNNIFKIIEGDIEKWEKTY"
     gene            7933..8451
                     /locus_tag="PKI16_02940"
     CDS             7933..8451
                     /locus_tag="PKI16_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNR53405.1"
                     /translation="MGKNILIHACCADCLLNTLSALKQQKDITVLFYNPNLHPRAEFL
                     ERLNAVKIVLQNYPNVKLVIPDYRPSEYFESIQKETKRCTGCWKQRLGYLFMYAKEHE
                     FESVTSTLISSSYQSREEILKIALALEKEFSIPFIQPKECKQEIHKGFYKQNFCGCCY
                     SLTERMNEKYLK"
     gene            8632..9348
                     /locus_tag="PKI16_02945"
     CDS             8632..9348
                     /locus_tag="PKI16_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNR53406.1"
                     /translation="MLSQKFRPSILLIICDANIGCLFQKNLLNTEFYVSIAYNAETGI
                     KKALSNKYDIVLIDLQEQPKECFNILICIRNVNRSIPIIILGEKEDIENQINTYKLGG
                     DLFHSKPISFPLIEAEIRQLLFYRLKNVLVQMGDIEMDLTSRIIKRKGREIRFTRSEF
                     NLLLLLIKNRNMAISREKIISDVLNYNKDIEYAAVDTMVSRVRKKLSQYGDESVIDTV
                     VKVGYRLNPVYIKNCKIKHY"
     gene            9371..9862
                     /gene="def"
                     /locus_tag="PKI16_02950"
     CDS             9371..9862
                     /gene="def"
                     /locus_tag="PKI16_02950"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNR53407.1"
                     /translation="MPKALKIITIKDKEEEKLLREKSQDVSTEDIKTSEFQEFLDNLL
                     YTAQHSEEQGNVPAGGIAAVQVGEHKRIFFILNYDTNEWEVFINPKIEIEEFLKTTTK
                     EGCLSVPDILGDVTRYLKVKIKYQNREGEWETKRYEGLNAVSIQHEEDHLEGVLFIDK
                     MEK"
     gene            9883..>11000
                     /locus_tag="PKI16_02955"
     CDS             9883..>11000
                     /locus_tag="PKI16_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53408.1"
                     /translation="MFKRIIITVILTVIFLGNKGVLAQQSVEDVIGTVKAETVISDLD
                     QKEALREESFSNLLKLNLPSQTNNPSLVLTFTDPSEDKKGVQLEIDSKSFVTIKSPYS
                     LPALSIGDHILKFKFVDNVGTTQLLEKTLIVLPRAPIISAPTISDSSVLVYGTGLANS
                     EIILVMSSGLNLFLKQSTIDAEGKWSVPFEAGELVDGTYSLTGYVRKYGYSSDPAEAI
                     TFILGEGTTTIAGTNSNAFAFKNVDVSTLGKFVSDNPDIIALVAGFFVLGFLVSFLLS
                     LIFKNTDDDKDIEKMKKDVVKPKIEGKELTLKEKLLLGKKEEKPPVEEKKKEEEKVEV
                     KKDEKPEVKEEEKIVTKIDFLRDFKNFDPDNAKGKESN"
BASE COUNT         3993 a         1866 c         1950 g         3191 t
ORIGIN      
        1 aaattactgc acaaagtgaa attaaaacag aaaaagatgc atacaatata tctaaaaaga
       61 gagccaaaga attaaaagat ttaggtataa ctatgaactt ctcaccagta ctagataata
      121 tacagaaaga agattccttc ctgtatgacc gagtatttag aacagatata tctttgcttg
      181 gtaacaagat gatctctgga tacacagacg gaggagtcat cgctgtgcca aaacatttcc
      241 cagggcatcc agataattca attgactctc acaaaacatt gccggaagta aatacccctg
      301 aggcagactt tgatacatat gcatctcaat ttaaagaggt tctctcaaac ccagatattc
      361 aagccattat ggttggacat attcttttta aacagataga cgcaaacaat ccttcatccc
      421 tttcttctaa gattattaca gaaatcctaa gaaacaagta tggctttaaa ggagtagtca
      481 taacagatga tatgcagatg ggagcattaa cagaaagttt aactattgca caagctgcag
      541 ttaaagcagt tactgcaggc aacgaccttc taatatattc tggagagcca gaacaacagg
      601 cagaggcata caatgcaatt ttagatgcag ttaagaaagg taagatatct gagcagagga
      661 taaatgaaag tgtactgaga atactcacat taaaagcaaa tatatttaat ttgtaattcc
      721 ttgagttatg caaaagaaat tagcaatact ttcttttctc atactactgt tccttctagt
      781 tctaactggt gtctgggcac tcttcattag gaaagacgac acttccaacg attggaagac
      841 gttaactgag gacaatatca ctctcacata tcaatatgct ggagttaatg tatggaaata
      901 caatgtaaaa ggacagcttc caaatccgtg ccatagtgcg caggtagatg cactagttgc
      961 agaaagctat ccagaacagg taactgttaa ggtaaatatc gaagagaaca agagtacaga
     1021 tactgtgtgt gcacaggtta ttcaggagtt gaatattagt ggagaattca atgcttctga
     1081 gaaagctact gtgacattac aagttaacaa acagtaattc taaaagctgt ataatgtatt
     1141 atgctaacaa aatcagatta cattacatac cttgactctc ctttacacct gtgggcgctt
     1201 aagaataatc agtatgaaag aaagctctct gactttgaca aacatctcat aaaacaaggc
     1261 tatgaagtag agaccttggc aagagagtat gtaaagaaat atatttcaaa agatgcacaa
     1321 taccaaaaga tataccaaac ggatgatctc tttgcacgag cagatattgt agttggccat
     1381 gatatatacg aagttaaaag ctcaacagaa atgtcaaaag aagatgagca cgatatctcc
     1441 ttccaatact acttagcaaa aacactcggt aaagttgaaa acatctacct tcttcaccta
     1501 aacaaagatt atatacgagg aaagaaacta gatttaaagg aattgtttgt tgtagaagat
     1561 gtaacaccac ttgctaaaaa gaattggagc tacacggaag caacagtagc tgaagctatg
     1621 ctcataacta acaaggagat tccacaaggc ctgtctccat gctacgaccc caaaagctgt
     1681 ccctctaaag ctctatgctt cccagatact ctaccagagt attccatcta cgatgtctct
     1741 agaattagta aaaagaacat tgatgctctg agggagatgg gtgtagtaga tatgcgaaat
     1801 atcccttctg aatttaacat agctcaaaag caaaagctgc aaattgaggc aaccaaaaaa
     1861 gacaagattg tcattgatga acaaaagatt ctttccgaat tggatagttt ggaattccca
     1921 atatattttt tagattacga aacatactcg tgggctgtcc cacaatttga aggacacaaa
     1981 ccatacgaaa atgtcgtgtt tcaattctct cttcatcttc taaaaaagaa agatggtaac
     2041 ctatcacact atgaatacat agccattact aaaaaagacc ccatacttga tgtcctgaat
     2101 atacttcagg atgtaatagg ggataatgga agtatattgg tctggaacaa aacttttgaa
     2161 aaaggttgta atgaggagat ggcaagaata tatcctgagt atgagcaatt tcttcttaat
     2221 ctaaatagaa ggatatatga tttaggagat atatttaaat accagatgta cgtagatcct
     2281 aagttcaaag gtagttggag tattaagaat gtgctaccag tattagcccc cgatttaaat
     2341 taccatgata tggagatagg aaaaggtgat gaggccatgg ttaactggta tcgcttatct
     2401 tacgagaatc aatctcaaga ggacacagat agtacaagaa agaacctttt gaaatattgc
     2461 gaacttgata catacgctat gtataggata tatgatttcc ttgtatctaa agtgttttag
     2521 agatataatt actccgattc aaaatactaa taacaggaga gtgtgaaatg gagtctgaac
     2581 ttttgtttgg gaaatttaaa acgaaagaag aggcaataga gtgggcgttg gaaattctaa
     2641 cattagaaac tccattctgc ctagaaattc tggaagaaca agttgaaaaa gcaaaaaaaa
     2701 tacttttaac ttatctaccg gatccatggc tcggagtaga atacaaaggg aaaattttcc
     2761 ttaaagaaaa atacaatcca tttgttctct ctccagcaca agctgttggt attataaata
     2821 aatagtaatt tgtttcaaat acaggaaggt tatccttcaa aataatcttc ctgtatctat
     2881 tccatttttc ctttcttcta tgtacaataa aatatagtaa ttttatatat tcatacagta
     2941 tgaacaaaga actcctcatt aaattgtccg ttacagtatc tgtgattgct atgttcgtag
     3001 taaatgcact agcaaatatc ctaccaatta acggcatgac aacaggtgca ctatctgatg
     3061 ctattcctat ctactttgtt cctgcaggat atgtgttttc catctgggga ttaatatatg
     3121 ctgggttagt ggtatacatg atttctatgt ttaccaaatt taccaaagaa gatgaagcaa
     3181 tatatccttg ggtaatcata agtagtcttg caaatacagc atggatattc ctttggcact
     3241 atagattaat atatgtatct gtaattgcca tggttataat ccttgccagt cttattgcca
     3301 tcgccttaat tcttaacaaa tccaaaaagg tctctatctt taaaacactt ccatttaata
     3361 tctacttagg atggatatct gtcgcatcaa tagcaaacat tgcagctgct ctgtatctgg
     3421 caaattggaa tggatttggt atttcaccag aagtttggtc cgcaattatg attgttatag
     3481 caacaggact tgcagtatat tcaatcctta aaaagagata cgcttactcc cttgtaatcc
     3541 tttgggcggt aatcggaata cttgttaaat tctctgggtt atctaattcg atagaagtag
     3601 catctacact tgggatattg gcaatatcaa gtgtaattgg attcaaatta tattttggaa
     3661 caaaaactac aaagtaacag cccacaagcc atgaacatta cagtatgctc taacttttag
     3721 atccttttcg cgtttgactg agaattctgc atacggttta tcatttggga gaagatattt
     3781 tctagataca ttctcatcta cgatacattc tatccattca atgtaatgct cttctatcat
     3841 tggatggtct acagaaccga catttacctt taacattccg tcttcaaatg tagctactgg
     3901 gagatgcttt tccatgcctt cttcagcaga tttctcctca agtaattcca ttggtttgcc
     3961 acaacacaca agagttccac cacctacatg tgtaagctga acaatgttcc cacatattgg
     4021 acatctgtat atctcattga tttccatcgt taaatgtatt aaatttagct gtattaaagt
     4081 ataacttgtt aaagaagaaa ataaaacatg acaaccgcta tacccgtttt tattgggata
     4141 ttgttctgtc ttcttatttt gtttttttcc gggaaatctt tagttacaat atctattaac
     4201 cttgcaagaa aacttggtgt cagcgatatc ttcataggga ccacattgat agcatttaca
     4261 actgggctac caacattggt agtaagcatt attgctttct catcaaatca gcctgaaatt
     4321 gcgattggaa acctaattgg tacaaattat gtgaatatcg gattagctct aggaattcca
     4381 gcttttttga ctaccatctt agtaaaaaaa gaagtctttg agaaagaaat ccctttgtat
     4441 ttctccttaa tggcattgtt tacatcgttt atcttggaca tgagaatttc cacactagaa
     4501 ggaattcttt taattgtttt tctttttgct gcctggggta ttatcataca atatgggaaa
     4561 tcacacaaag ttcctcaaca ggatttagag cttaatccct ccaaagagaa aattaacaag
     4621 cgagactacc tatctttatt acttctattc ctcgcaattt tagtagcctc ttttggtata
     4681 tccttcttaa caccattact tgcacaacat actaatatta gtagttactt acttggactt
     4741 actctcatcg gagttggaac atcaatacca acaattacag cgggtataac agctgcgaaa
     4801 cagggaaaca cagacattat cgttggtaat gtctttggag gaaatatctt aaacatttgc
     4861 cttgggatag gtcttcttgc catgattaag cctatgcaaa tggacagtaa cgcagtaaat
     4921 gatttacttt ttgtaaatat ttatggtgcc attattgtaa tccttatttt ggcagagatg
     4981 aaattcttag gtaaaaacaa aacattaagc caaatatccg gtctaattat ggttcttaca
     5041 tatcttggtt atctagcaat atctattgct aagtatttct aatagactat aataacgtta
     5101 acatttgtga tacaatctcc ctttgtcatg gaatactctc cagttaataa agaaaatata
     5161 agaaatgtcg ctattatcgc acatgtagac cacggtaaga ctacccttgt ggatgctttt
     5221 atgaagcaaa cccatctttt tagagataat gaggaagaga tgggacaaac aagaatatta
     5281 gattccagtg atttagaaaa agagaagggt attacaatta gtgctaaaaa catttccatt
     5341 agatacaaag gatacaaaat aaatataatc gacaccccag gacatgcaga ttttggtggt
     5401 gaagtagagc gaacattgaa tatggcagat agttgtcttc ttttggtaga tgcaaaagag
     5461 ggtcctatgc cacaaacaaa atttgtcctt aaaaaagccc ttcaacttgg attaaaaccg
     5521 atccttgtta ttaacaaaat agataagaaa ttagcaaaca acaaaagaac tctcagtaaa
     5581 gttcaggacc tttttctagc attagcttca gatgttgagc aactagagtt cccagtttac
     5641 tacgccatat caagagaagg aaaaattttc aaagaaatac cagaaggcga tcttactatt
     5701 cctaattcta ctactggaaa tgtatctcca atcctagatg caatcataga aacaccagaa
     5761 ccaactggtg aattagatgc accatttcaa atgcagatta attccttaga atatgaccaa
     5821 catcttggaa gatatttaat tggaaaaatt agaagaggta aggtaagtat caatgatcca
     5881 atcattcttc ttacaaaaga agacaagaaa cagggtagag taaaagaact ttttgtaaaa
     5941 gaaggacttg cctggatacc agcaaagagt gctgcagtag gagagatagt tgcgctaaca
     6001 ggattagaat ctacagccat tggagctaca atatgtgctc tagaccaccc agaaccactt
     6061 ccagagataa caataactcc accatcagtt agaatcaagg tagaaccaaa cacatctcca
     6121 ttcttaggta aggaaggaaa gtttgtaaca gcaaaacagc taggacaaag acttgagcag
     6181 gagaaaaact taaatattag tttggatata caaagccatg gctcaaatag ttacttcata
     6241 gctggaagag gtgaacttca gttggcaatt cttgtcgaac agttaagaag agaaggcttt
     6301 gaattacagt taagtaagcc agaagtaatt cttcaaaaaa ttgacaatgt agtctccgaa
     6361 ccacttgaag aattaataat cgatgcacca gagatataca tgagtacaat aacccaagag
     6421 gtaagtgata gaaaagggga attggttaat attgaaacag aggaagggaa cacacgattt
     6481 acatacaaaa tattaacaag aaatattatt ggtttacaca gagtccttat gaacgctaca
     6541 aaaggtgaat ctattattaa cagcttcatc acagaataca taccatatac acagcaagaa
     6601 tctctcttca gaagaggtgt aatcatatcc tccgaaactg gaacagcact tgcatactcc
     6661 ctaactacag ttcaggagag aggaagactc ttcatagaag gagcaacaga agtgtacgag
     6721 ggtatgataa tcggaattaa caatcacgag aacgacattg aggtaaatcc atgtaaagca
     6781 agacataaga caaacgtaag aatgtcacac gcagaagtaa cagagatatc tcttaaagct
     6841 aaattaccac ttacattgga atttgcactc tcatttataa atgatgatga actaatagag
     6901 gtaactccaa aaagtattcg tttaagaaag aaacttctaa ccgacactga gcgagtatgg
     6961 gcaaagagaa aaaatctcac agcctatgct aaacagcaaa tgggtataga gtaatcatta
     7021 aatccaaata gctatataaa agaaaaaggg ctgttttacc agccctttta gaattcaatt
     7081 aataacagaa ttagtttctg tcgtctctat tgaagtttcc gccatcaaat gatcttctct
     7141 cacgattttc ctcacgtgct tcagatacat tcaaagctct accgtccaac tctttaccat
     7201 tcaactcagc aattgctttc tgagcttctt cttctgagga catttttaca aaaccaaatc
     7261 ctttggatct gttcttgatt ctgtccttga ttactactgc ctcttcgaca gtaccaatgg
     7321 cactaaaagt ctctcttagc atctcgtcat cgacgttcca gctaagattt ccgataaata
     7381 atttgtttcc cattatttat aaaaaataaa ataaactcgc tataccaata cacttgggag
     7441 aaaaattact caaagcagtc tcaacgatcg ctccaatagc atgaacatta tagcatagtt
     7501 tgtagtaaaa tatataccta tgagactttg gagtatttct tttaaatatt tggacagaat
     7561 gggacttctt gcagtctggc gtgaagcact tcttgcgaag aaagttttgg aggataaaac
     7621 aactggatat cggaatcatt ctcaacttgt tcgttttaaa aatacaaaca atccagtaaa
     7681 gtatataaac gcatatttag aagatatata taaagaagca caagcaaggg gttattcttt
     7741 ctcaaaagag aaattttcag aagagataat tgataaacat attcctgtaa attcgagaca
     7801 ggtagaatat gaatttacac atctaaaaaa caagcttaag agaagagatc ctgtaaagtt
     7861 caaagagctt ttagatataa cacatattaa agttaacaat atttttaaaa taattgaagg
     7921 agatatagaa aaatgggaaa aaacatatta atacatgcct gttgtgcaga ttgtttactt
     7981 aatacactct ctgcactcaa acaacagaaa gatattacag tacttttcta caacccaaac
     8041 cttcacccaa gagcagaatt tttggaaaga ttaaatgcag taaaaatagt tttgcaaaac
     8101 tatccaaatg ttaaattagt aatcccagat taccgtccaa gcgaatattt tgaaagtata
     8161 cagaaagaaa ctaaaagatg cactggatgt tggaaacaga gacttggata tctttttatg
     8221 tatgccaaag agcatgaatt tgaatctgta acttctacgt taattagtag ttcataccaa
     8281 agcagggaag aaatacttaa aattgcatta gcgttagaaa aagaattctc aattccattc
     8341 atacaaccaa aagaatgtaa gcaggaaata cacaaaggtt tttacaaaca gaacttctgt
     8401 ggctgttgtt actcacttac tgaacgaatg aatgaaaaat atttaaaata actgctataa
     8461 tttcccttat ggattacttt gtcataatat tacttgccct gttggcatct gttgcaattt
     8521 taattatcct ttctcgtttt cttccaaaca aagatacaga ggatctcgat gaagaagaag
     8581 aataaaaata ttgtaaggtt tttgtaagac aaaggcttat aaactttcaa gatgttaagt
     8641 caaaaattta gaccttcaat tcttctaata atttgtgacg caaacattgg ttgtttattc
     8701 caaaaaaatc tccttaacac agaattttac gttagtattg catacaatgc cgaaactggc
     8761 ataaagaaag ctttgagtaa taaatacgac attgtattaa tagatcttca agagcagccg
     8821 aaagaatgct tcaatatcct aatctgcata agaaatgtaa ataggagtat tccaatcata
     8881 atattgggag aaaaagaaga tattgaaaat caaattaaca catataaact tggaggagat
     8941 ctttttcatt ctaagccaat aagtttccct ctaatagaag cagaaattcg acaacttctt
     9001 ttttacagat taaaaaacgt gctcgtacaa atgggagata tcgagatgga tttaacaagc
     9061 agaattataa aacgaaaagg tagagaaatt agatttacaa gaagcgaatt caatcttcta
     9121 ctattgttaa taaagaatcg caacatggca attagccgag agaaaataat ctcagacgtt
     9181 cttaattata acaaagacat agagtatgct gctgtagaca ctatggttag tagggtaagg
     9241 aaaaagcttt ctcaatacgg tgatgaaagc gtaatagata ctgttgtaaa agtcggctac
     9301 cgattaaatc ctgtttatat taaaaactgt aaaatcaaac actattaata attatgatat
     9361 actcttggat atgccaaaag cactcaaaat aataacaatt aaagacaaag aagaagaaaa
     9421 attactaaga gaaaagagcc aagatgtttc tacagaagat ataaaaactt cagagtttca
     9481 ggaattccta gataatcttc tttacacagc acaacatagt gaagaacaag ggaatgtccc
     9541 tgcaggaggt attgctgctg tacaggtagg agaacataaa agaattttct ttattcttaa
     9601 ttacgataca aatgaatggg aggtcttcat aaacccaaag attgagatag aggaattttt
     9661 aaagactact acaaaggaag gttgcttaag tgttccagat attttaggag atgttacaag
     9721 atatttgaaa gtaaaaatta agtaccaaaa tagagaagga gagtgggaaa caaaaagata
     9781 tgaaggctta aatgccgtaa gcatacagca tgaggaagac cacttggaag gagttctctt
     9841 cattgataaa atggaaaagt aaattattaa gaagctgccg ccatgtttaa gagaataata
     9901 ataacagtaa tacttacagt aatctttctt gggaacaagg gggtacttgc tcaacagagt
     9961 gtagaggatg ttattggtac agtaaaagca gaaacagtta tttcagattt agatcaaaag
    10021 gaggctctga gggaagaatc tttttcaaat cttcttaagc ttaaccttcc aagccaaaca
    10081 aataatccta gtctggtttt aacctttacc gatccatctg aggataagaa gggtgttcag
    10141 cttgaaatag attccaaaag tttcgttacc attaagagtc catatagtct acctgcactt
    10201 tctattggag accatatctt gaaattcaaa tttgtagata atgtagggac aactcagctt
    10261 ctagaaaaaa ctttaattgt actaccaaga gctccaatca taagtgcgcc aacaattagt
    10321 gattcatctg tacttgtgta tggcactggg ttagcaaatt cagaaattat tcttgtaatg
    10381 tcctctggct taaatctctt tcttaagcaa tccacaatag atgctgaagg aaaatggtcc
    10441 gtaccatttg aagcaggaga gcttgtagat ggaacatact ctttaacagg atatgtaaga
    10501 aaatatggat attcaagtga ccccgcagaa gcaattactt tcattcttgg agagggaacc
    10561 acaacaattg caggtactaa ctcaaatgct ttcgctttta agaatgtcga tgtctctaca
    10621 ttaggaaagt ttgtatctga caatcccgac atcatcgctt tagttgcagg attctttgta
    10681 cttggtttct tggtgtcttt cctgctttct ctcatattta agaatacaga cgacgacaaa
    10741 gatattgaga agatgaagaa agatgttgta aaaccaaaaa tcgaagggaa agaattaaca
    10801 ctaaaagaaa aattgctttt aggaaaaaag gaagagaagc ctcctgttga ggagaagaag
    10861 aaagaagaag agaaagttga agtaaagaaa gatgagaagc cagaagtaaa agaggaagag
    10921 aagattgtta caaagataga ttttctaaga gatttcaaaa actttgaccc agacaatgca
    10981 aagggcaaag aaagcaacaa
//
LOCUS       DAOQWP010000021        10787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_6565_length_10787_cov_8.260995, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000021 DAOQWP010000000
VERSION     DAOQWP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6565_length_10787_cov_8.260995"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..775
                     /locus_tag="PKI16_02960"
     CDS             119..775
                     /locus_tag="PKI16_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53409.1"
                     /translation="MQPNPELETEKETTIGRDLLLSTSENIFHTRVETSQSKEYKNYK
                     ITEFSVFKEEIQSASSEDIWINYSGLVGESLFLLACENNNIPIKISTGDEDWAGIDFY
                     LFGYPIDVTTACKQHAIERKIDTTRYPTLFLPRFVGNNSIYCKPPVLIPYTKELFEKG
                     SFDTTRYLDEMLQINYEVEEIIENEVYFKNEQSIHLRKAGLNNVHNMKTIMTLISGIL
                     "
     gene            complement(755..2431)
                     /locus_tag="PKI16_02965"
     CDS             complement(755..2431)
                     /locus_tag="PKI16_02965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNR53410.1"
                     /translation="MQTIRMTERLEKKKTDTKKSIKLAFILFLLIFLPYMYISNMKTL
                     NILIFTRQVKVVYNTFSVSFEKDIPTEYVDLVKEKLGSIAFNEKRRFEFVEGKSDIVV
                     SSTPSEGSVDVFKKDLIPVGHVYSLLNEIAEKDVQKYNLYILDSQYIGYVKAQYGVEP
                     KVVTSIEELITTLKKSDSNIGLVEFKDLSPSFKILRVDSHYYLDDNEGSIPIRFYATI
                     DNQDEAFILAVMKKNLSDYGDDWSKESLAKINMSGVVAISRGLAYKMDSLKNYDYPAE
                     KLGSFLADADLTHISNEVSFTDTCKVYSGMRFCSNTKYFETLTASGVDIVELTGNHNN
                     DFGSVNNAKSIQMYKDAGMRYFGGGLNKDDASKILYEEVKGTKIAFLGYNYYDSMLKT
                     GAIAWGSTAGANSYTEAKMKADIEEARKNADIVIVDFQFQECYSYPSSDVVYPICYKP
                     LSSPDQKGTFRKAIDFGADIVIGTQAHQPQTYELYGNGVIYYGLGNLYFDQKYWIGTR
                     QGIVLTHYFYKGKHIQTKLTPIYMDSSLQPELATKTESEQLLKSLKNARN"
     gene            2542..3105
                     /locus_tag="PKI16_02970"
     CDS             2542..3105
                     /locus_tag="PKI16_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNR53411.1"
                     /translation="MKKLLLKGRTAVAILSGLLFVGMIFKTIDDKIPTQIAQANQNTV
                     LPTKEETINESIQIEAVVTQKVADKRVIQIKQYLSKRNAPLAKYAQDFVDAADKYGID
                     YRIVAAISIIESGGGKNTFKPYNAWGWGKSGFANWKEGIYAVSKGLGKYYDIGLTTPK
                     LISKSYCPPSADSWASKVQYVMNEISK"
     gene            3129..3815
                     /locus_tag="PKI16_02975"
     CDS             3129..3815
                     /locus_tag="PKI16_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNR53412.1"
                     /translation="MEPKVAKFEKYLLTYFNKAELNILLDEIFNKKIYDIDLETTTPI
                     IFDVGAHIGLATIFFNQKYPLARITAFEPNPNTFPLLEENTYFNNIHNVTLHNVALGK
                     KSSQRTLFIDSSNNGSFSTASFMKDAWNGTQESLGIEVITEPLSKYINSTIDLVKMDV
                     EGTEQEIIEELEESGKIGNIKNIIIEFHPTEKQDIEKIKSILQKNGFTIEIRKDKKDK
                     LVYILGKHSL"
     gene            complement(3783..4808)
                     /locus_tag="PKI16_02980"
     CDS             complement(3783..4808)
                     /locus_tag="PKI16_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNR53413.1"
                     /translation="MNVTVIGGGTGSTVVLEGLKEYRDLNLSVIVGMMDDGGSNAVVR
                     DEFGLLPLSDVRKSIVALADSGNNDMLRKLFTYRFSEGENIKGHTMGNLLMIAMTEIM
                     GSEVEAIEMFKVMFNIRGNIFPVTLDDVRLVAEYGDGTKVVGEHLIDEMKEDKKITNF
                     YLDSKAKIYPGAKECIERSEYIILGPGDLFTTMLPNLLVDGVSSALKKSKGKIIYISN
                     LMTKIGQTRHRSHKEIVELIEGYIGRKIDYILINNGRIPEEAYKRYVADGESLIIDDL
                     EKTKKRKIIYADLVANDVIKKDKGDKLVRSLIRHDSKKLAKELYKIFNDSSIRRFLKG
                     VFSQYID"
     gene            4867..5391
                     /locus_tag="PKI16_02985"
     CDS             4867..5391
                     /locus_tag="PKI16_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNR53414.1"
                     /translation="MELKIGSKVFYPSHGAAKVIAEKEIEFNGEKKKYFEFEFMTNPL
                     SISTPVENIASLGIREVLPVAKIKDAMKELKKNSTKNPKVKDFNVMLNIFKDLESSSD
                     IKASVEIIQYCNYVKKTREKEGRLIPISIEKQLEEAVKNIVGELALATDKSLEDASKT
                     FSKATGITVDVIKD"
     gene            complement(5381..8257)
                     /gene="ileS"
                     /locus_tag="PKI16_02990"
     CDS             complement(5381..8257)
                     /gene="ileS"
                     /locus_tag="PKI16_02990"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNR53415.1"
                     /translation="MSKKEELKNVSNNMDFVDMEKKVMELWKEQDIEKKYVEKNKGAK
                     ENFSFIDGPMTANNPMGVHHAWGRTYKDVVQRYKNMQGYEQRFQNGFDCQGLWVEVEV
                     EKELGFNSKKDILNYGMDKFTESCVARVNHFAGVQIEQSKRLGMFMDWDNSYYTMSKT
                     NNLYIWYFLKKCQEKGLIYKNKSATTWCPRCETGLSQHEQAEGYQMITDTSVYLFFKL
                     SNRDNEYVLAWTTTPWTLSANVLLAVNPELEYVKTNIDGKTVYLGADAAKRLGLNNVE
                     SIDVANLMGEKYDSLYDFESQKGVEHKIVEWDLVDAKSGSGVVHIAPGCGQEDYELGK
                     ELGASAISPIDSTGRFLEGYGDLTGIYAHDVSDIVIGYLQDKGLLFKTEEYEHSYPHC
                     WRCKTKCLFRLEDNWFINVQKIKAQLKEQTDTVKWNPEFVGKRMHGWLDSMGDWMISR
                     KRFYGLALPFYECSKCNTLHVVGSLEELKELAVDPSLVDNLKSIHRPWIDEIKIKCPH
                     CGQEVNRVTDVGDCWLDAGVVPFSTLNYLEDRAYWEKWFPADFITEMLEQVRLWFYSM
                     LVFGVVLEGKVPYKEVLGYAELRDENNERMSKTKPNNIKFDEAADKAGSDILRWNYVT
                     ASIGGNMRFGWPTLDDVKRRFYLPLWNSFNYLLTYSKLHNFDYSRFDIAEVDNVMDKW
                     IIARLHKLVNEVIGYMDGYNIASSSREIEDFVKDLSQWYIRRGRVRFKNGDANALGTL
                     HYVLLELSKLLAPFVPFISEVMYLNLSGEKESVHLENYFDSKKENIDEDVLKKMNLAR
                     EICSNGLKVREDAKMNLRQPLLEAYISIDDSEIMEIVKEELNVKSIFYAKEQPKGECI
                     KSFGNAEKLVSINTILSDDLEKEGMLNDFLRKYRDIRKKQGLKINDTVSLVLFTESEL
                     LKEVVDGCGKTTLQVKDIQFVDDISKTENQFKVGNVQVGLTINL"
     gene            8565..10328
                     /locus_tag="PKI16_02995"
     CDS             8565..10328
                     /locus_tag="PKI16_02995"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNR53416.1"
                     /translation="MNWNLKTLYTSEKDPQIQKDILLSKKANASFITKWSKNKKYLQD
                     PKVLLQAIQEYEKLEETYGQCQKPLYYLSLRSYLNQKDSVIKGMLNKLSETATKMANN
                     IQFFELNISKIPKEKQKEFLNYKGLNNYKHYLEKLFLVGKYLLSDNEEKIFNLMSKTS
                     HSNWTDMVEELLNKQKFTVKDEDGKNKEISYNEISKYLNSTNKKVRDYASNEFNKINS
                     KYAEIAEFEINSILEAKKVSDDYRKIPRPDLTRHIADDIDSETVDTLVKVVTDNFNIS
                     QQYYKKKAKLLGLQKLGYHERNIPLKGIDIPYPYKKGMALVKETFSSLDSKFEEILNS
                     FEKENIYDVYPKESKSGGAFCISVGRSLPTYILLNYTDRLNDVLTIAHESGHGIHSIL
                     SKKQTELNSGHPISLAEVASTFFEDFVLEKILEKTNDKEVLLGILDNKLNGDISSIFR
                     QIAFYNFEKELHATYREKGFVPKEEISEMFCKHMTAYLGDSVEQDESMRNGWIYVSHF
                     RRFFYVYSYASGLLISKALQKMVKDNPKNISYVKAFLESGGSKSPKVLFKEMGIDISK
                     EEIWKKGIKEIKYLLDKLDGI"
     gene            10331..10735
                     /locus_tag="PKI16_03000"
     CDS             10331..10735
                     /locus_tag="PKI16_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53417.1"
                     /translation="MNQDNWWEGGSQDRTDQTLEQYSQDVQEYLETEGIKGYLLGDPT
                     ICGYTTMQVCVLDPQEDIYNNVLLFATQITKFNDPEFKLQPLKKDSYNYIYDNFHKDY
                     QLELQSEGVDNKIQSFQGRFDRKAKITEMREI"
BASE COUNT         3633 a         2063 c         1596 g         3495 t
ORIGIN      
        1 aaacaagaca agggggtgaa actatttttc ataaatacag ttttaccccc tcagaaatac
       61 tctatactca ttgactatca cttccctttc tctgtacaat gtaaattatc aagaaataat
      121 gcaaccaaac ccagaacttg aaacagaaaa agaaacgact attggaagag atttactact
      181 atctacttcc gaaaatatat tccatacaag agtagaaaca tcccaatcca aagaatacaa
      241 aaattacaaa ataactgagt tttcagtatt taaagaagaa atacaatctg catcttccga
      301 agatatatgg attaattact caggattagt tggtgaatct ctatttctgc ttgcatgcga
      361 gaataacaat atccccatta aaatatctac tggagatgaa gattgggcag gtatagactt
      421 ttatctattt ggctatccca tagatgtaac aaccgcatgt aaacaacatg ctatagaaag
      481 aaaaatagat acaacaagat acccgactct ctttcttcct aggtttgttg gaaataattc
      541 aatatattgt aagcctcctg tacttattcc ttacacaaag gaactttttg aaaaaggatc
      601 ttttgacact acaagatatt tagatgaaat gctacagata aactacgaag tggaagaaat
      661 aattgaaaac gaagtatatt ttaaaaatga acaatcaata catctcagaa aagccggctt
      721 gaacaatgta cacaacatga aaacaataat gacactaatt tctggcattc tttagagatt
      781 ttagtaattg ctcactctcg gtttttgtag ccaactctgg ttgtagtgaa ctatccatat
      841 atataggggt taactttgtt tgtatatgct tacccttgta aaaataatgt gttagaacaa
      901 tcccttgcct tgtccctatc caatacttct gatcaaaata caaattaccc aaaccataat
      961 aaattacccc attaccatac aattcataag tctgtggttg atgtgcctgt gtaccaatta
     1021 caatatctgc tccaaaatct atagcctttc taaacgtacc cttctggtct ggagaactaa
     1081 gaggtttgta acagattgga tacactacat ctgaactagg atagctgtaa cactcctgaa
     1141 attggaaatc tacaataaca atatctgcat tctttcttgc ctcctctata tctgctttca
     1201 tttttgcctc tgtgtatgaa tttgcacctg cagtactccc ccacgctata gcacctgttt
     1261 tcaacatact gtcatagtaa ttgtaaccca agaaagcaat cttcgtacct ttaacctcct
     1321 catacaaaat tttagatgca tcatccttat ttaacccccc accaaaatac ctcataccag
     1381 catccttgta catctgtata ctcttagcat tgttaacact accaaagtca ttattatggt
     1441 tgcccgttag ttccacaata tctacaccac ttgctgttag cgtttcaaaa tatttggtat
     1501 ttgaacaaaa cctcatacca gagtaaacct tacaagtatc tgtaaatgac acttcgttag
     1561 aaatatgtgt aagatctgca tctgcaagaa aactccctaa cttctctgct ggataatcat
     1621 agttttttag agaatccatc ttgtatgcta aacccctaga tatcgctact actccagaca
     1681 tattaatctt tgctaaactc tccttactcc aatcatcacc atagtcagag agatttttct
     1741 tcataacagc aagtatgaaa gcttcatctt ggttatctat agttgcatag aatcttatgg
     1801 gaatagaacc ctcattgtca tctagatagt aatgactatc tactcttaaa attttaaaag
     1861 agggacttag atctttaaat tccaccaaac caatattact atctgacttc tttaatgtag
     1921 ttattaattc ttcaatactg gttacaacct ttggttctac accatactgt gcttttacat
     1981 acccaatata ctgagaatct agaatataca aattgtattt ctgaacatct ttttccgcta
     2041 tttcatttaa caaggagtag acatgcccaa caggtattaa atctttctta aaaacatcta
     2101 cagagccctc agatggagta cttgagacca caatatctga cttaccctct acaaattcaa
     2161 acctcctctt ttcattgaat gcaatactac ctaacttctc ctttactaaa tccacatatt
     2221 ccgttggaat atccttttca aaagacactg aaaaggtatt gtaaacaacc ttaacttgac
     2281 gtgtgaatat taatatattc aacgtcttca tattggatat atacatgtaa ggaaggaata
     2341 tcagcaagaa gaggataaat gccaatttaa tgctcttttt tgtatcggtt ttcttcttct
     2401 ctaatctttc agtcatcctt atcgtttgca tacgctccag tttatcacaa tatgaccaat
     2461 ctgtagacaa tacacactat gggctaaata tggtataata tgtgttgtca taatggcgtt
     2521 ttaactttaa aaatactaat tatgaaaaaa ctgctattaa aaggaagaac tgccgtagct
     2581 atactatctg gactactttt cgtaggcatg atattcaaaa ctatagatga caaaatccct
     2641 acacagatag ctcaagctaa ccaaaataca gtacttccta caaaagaaga aaccattaat
     2701 gaaagcatac aaattgaagc tgttgttact caaaaggttg cggataaaag agtaatacag
     2761 attaaacagt acctttctaa aagaaatgca cctttagcta aatatgctca agactttgtt
     2821 gatgctgcag ataaatacgg tattgactat agaattgttg ctgctatctc cattattgag
     2881 tctggtggtg gtaaaaacac attcaaacct tacaatgcat ggggatgggg taaaagtgga
     2941 tttgcaaact ggaaagaggg tatctatgcc gtatctaagg gattaggaaa atactacgat
     3001 attggtctaa caacaccaaa attaatatcc aaatcctact gtcctccaag tgcagacagc
     3061 tgggcaagta aggttcagta tgttatgaac gaaatatcta aatagggata tttaatatat
     3121 aatcagatat ggagcctaaa gttgctaaat ttgagaaata tttacttaca tatttcaaca
     3181 aagcagagct taacattctc ctagatgaaa ttttcaacaa aaaaatctac gacatagatc
     3241 ttgaaactac cactccaatc atctttgatg taggagcaca cattggttta gctacaatat
     3301 tttttaacca gaaatatccc ttagcaagaa ttactgcttt tgaaccaaac ccaaatacat
     3361 ttccattgtt agaggaaaat acatatttca acaatataca caatgttaca ttacataatg
     3421 tagcactagg gaaaaaatca tcccaaagaa cactgttcat agattcttca aataatggca
     3481 gtttctctac tgcaagcttt atgaaagatg catggaatgg tactcaagag agtcttggta
     3541 tcgaggttat aacagaaccg ctaagtaaat atatcaactc tacaatagat ctggtaaaaa
     3601 tggatgtaga gggtacagaa caagagatta tcgaagagct agaggaaagt ggaaagattg
     3661 gaaatattaa aaacataatt atcgaatttc atcctacaga gaaacaggat attgagaaaa
     3721 tcaaaagtat tttacaaaag aatggtttta cgattgaaat tagaaaagat aaaaaggaca
     3781 aactagtcta tatactggga aaacactccc tttaagaatc ttctaataga ggaatcatta
     3841 aatatcttgt aaagttcctt tgctagtttt tttgaatcat gtctaattaa acttcttact
     3901 aatttatctc ctttatcttt ttttataaca tcattagcaa ctaaatctgc atatattatc
     3961 tttcgttttt tagttttttc taaatcgtcg attattaaac tctctccatc agctacatat
     4021 cttttgtatg cttcttctgg gattcttcca ttgttaatta atatatagtc aatcttcctg
     4081 ccaatatatc cctctatcaa ttcaacaatc tctttatgac tcctatgcct tgtttgacca
     4141 atttttgtca ttaaattaga tatatatatt atctttcctt tgcttttctt caaagcacta
     4201 gatactccat caactaaaag atttggtaac attgttgtaa agagatctcc tggaccaaga
     4261 ataatgtatt ctgatctctc tatacattct ttcgcacctg gatatatctt tgcttttgaa
     4321 tcaagataaa aatttgtaat ctttttgtcc tccttcatct catctatgag atgttcccct
     4381 actaccttgg tcccatctcc atactctgct acaagtctga catcgtctag tgttactgga
     4441 aatatattgc ctcggatatt gaacattacc ttaaacatct caattgcttc tacttcacta
     4501 cccattattt ccgtcatagc tatcattaaa aggtttccca ttgtatgtcc tttaatattt
     4561 tctccctctg aaaaacggta tgtgaaaagt tttcgcaaca tatcattgtt tccactatct
     4621 gcaagtgcaa caatggattt cctaacatca cttagaggta acagtccaaa ctcatctctt
     4681 acaacagcat tgcttcctcc atcatccatc attccaacga taacagagag attcaaatct
     4741 ctgtattcct tcaatccttc tagtactaca gttgaacctg taccacctcc aataacggta
     4801 acattcatac ggaaagtata ccactttcgt ggtataatac agcattaaca ttacttaaat
     4861 atcctcatgg agttaaaaat aggttcaaaa gttttctatc catcccacgg tgctgcgaaa
     4921 gtaattgcag agaaagaaat tgagtttaat ggagagaaaa aaaaatattt tgaatttgaa
     4981 ttcatgacca atcctttaag tatctcaacc ccagtagaga atattgccag cctaggaatt
     5041 agagaggttc ttccagttgc taagattaag gatgcaatga aggaattaaa gaaaaactct
     5101 actaaaaatc caaaggttaa agactttaat gtaatgctaa acatattcaa agatttagag
     5161 agcagttcag atatcaaggc ttctgttgaa attattcagt attgtaacta tgtaaaaaag
     5221 acccgagaga aagagggtcg tttaatccca attagtatcg agaaacaact ggaagaagca
     5281 gttaaaaata ttgttggtga acttgcctta gcaacagaca aatctttaga agatgcttca
     5341 aaaactttct caaaagctac tggtattaca gtagatgtta ttaaagatta atagttaatc
     5401 ctacctgtac atttccaact ttaaattgat tctccgtctt ggatatatca tctacaaatt
     5461 gtatatcttt tacctgtaag gttgtttttc cacacccatc tacaacctct tttagaagct
     5521 cactttctgt gaaaagtacc aaagacacag tatcgttaat ttttaaaccc tgcttctttc
     5581 gaatatccct gtactttctt aggaagtcat ttaacattcc ttctttttca agatcatcgg
     5641 aaagaatagt atttatagat acaagttttt cagcatttcc aaaagattta atacattctc
     5701 ccttcggttg ttcttttgca taaaaaatac tctttacatt taattcctct tttacaattt
     5761 ccattatttc actgtcatca atgctaatat aggcctcaag aagaggttgt cttagattca
     5821 tctttgcatc ttctcttact tttagaccat tagagcatat ttcccttgct aggttcattt
     5881 tcttaagtac atcttcgtct atattttctt ttttagagtc gaaatagttc tcaagatgca
     5941 cagactcttt ctctcctgag aggttgagat acattacttc agatatgaaa ggcacaaatg
     6001 gagccagaag tttgcttaat tccaagagta catagtgaag tgttccaaga gcatttgcat
     6061 ctccattttt aaatctcact cttcctcttc ggatatacca ctgagagaga tctttgacaa
     6121 aatcttcaat ttctctagat gaagaagcga tattgtaccc gtccatatat cctattacct
     6181 catttacaag tttgtgtaat cttgcaatta tccatttatc cattacattg tctacttcag
     6241 caatgtcaaa tctagaataa tcaaaattgt gcaatttaga atatgtaaga agatagttga
     6301 atgaattcca aagtggaagg tagaatcttc tttttacatc atctaatgtt ggccacccaa
     6361 acctcatatt tcctccaata gaggcagtta catagttcca tctcaaaata tcgcttccag
     6421 ctttatctgc tgcttcatca aatttaatat tattcggttt tgttttactc attctctcgt
     6481 tgttctcatc tcgaagctct gcatatccta aaacttcttt gtaagggact ttcccttcta
     6541 gtactacacc aaataccaac atagagtaaa accacaatct tacctgttct agcatttcgg
     6601 ttatgaagtc tgcagggaac catttttccc agtaagctct gtcttcaaga tagttaagag
     6661 ttgagaatgg tactactcct gcatccaacc aacagtctcc tacatctgtt accctgttca
     6721 cttcttgtcc acaatgaggg cattttattt taatttcatc tatccatggt ctatgaatac
     6781 tttttagatt gtctactaag gatggatcta ctgctaactc ttttagctct tcaagacttc
     6841 caacaacatg aagtgtgtta cactttgaac actcgtaaaa aggaagagct aaaccgtaga
     6901 atctttttct actaatcatc caatctccca tactgtctaa ccaaccatgc attcttttcc
     6961 ccacaaattc tggattccat ttaactgtat ctgtttgttc tttgagttga gctttaatct
     7021 tttgtacatt tatgaaccag ttgtcttcta acctgaaaag acatttagtc ttacatctcc
     7081 aacagtgagg atagctgtgc tcatactctt ctgttttaaa caacaatccc ttgtcctgca
     7141 agtatccaat tacaatatct gaaacatcgt gggcataaat accagttaaa tctccatagc
     7201 cttctaaaaa tcttccagta gagtcaattg gagatattgc tgaagcacca agttcttttc
     7261 caagttcgta gtcttcctgt ccacatcctg gagctatatg tacaacccct gaaccacttt
     7321 ttgcatctac aagatcccat tctactatct tgtgctctac acctttttga gattcgaaat
     7381 catacagaga gtcatacttc tctcccatca aattcgctac atctatactt tctacattat
     7441 tcaatcccaa tctctttgcc gcatctgctc caagatatac agtctttcca tctatgtttg
     7501 tctttacata ttccaattct ggatttactg ctaaaagtac atttgcgctt aaagtccacg
     7561 gagtagttgt ccatgcaagt acatattcat tgtctctgtt tgaaagcttg aagaacaaat
     7621 ataccgaagt atccgttatc atctggtatc cctctgcttg ctcatgctga gaaagtccag
     7681 tctcacatct tggacaccat gtagttgcag atttattttt gtaaattaat cccttctctt
     7741 ggcatttttt aaggaaatac cagatgtaga gattgttagt tttagacatt gtgtagtagc
     7801 tgttgtccca atccatgaac ataccaagtc ttttagattg ttcaatttgc actcctgcga
     7861 aatggtttac tcttgcaaca catgattctg tgaatttatc cataccgtaa tttaagatgt
     7921 ctttcttact gttaaatccc aattccttct ctacttctac ctctacccag agtccctgac
     7981 agtcaaatcc attttggaat ctctgttcat atccctgcat attcttgtat ctttgtacta
     8041 catctttgta tgttcttccc catgcatggt gtactcccat agggttgttt gcagtcattg
     8101 gtccgtctat gaaggagaag ttctcttttg ctcctttgtt cttttcaaca tatttctttt
     8161 caatgtcttg ctctttccaa agctccatga cctttttctc catatctaca aagtccatat
     8221 tgttagaaac attttttagt tcttcttttt tagacatctt tttgtgtttt taatttaaac
     8281 aaaaacccct tcagtaatat taacagtata tattaatatt accaaagggg cgaacattta
     8341 gtacgcggta ccaccctatt ttttacttat tgtgtatgta tcgcatacct acggtagatt
     8401 ctacttgtat atttactttc tttctactac tccccagtga tatctggatc tatgtattag
     8461 gaatattata tcagaagaaa aatttaaaag atatgagtga gatacacaaa gaagattaat
     8521 attccaatat gtccccatag tatcctttgt ggtatcatta caccatgaat tggaatttaa
     8581 aaactctgta tacatcagaa aaagatccac agatacaaaa agacatatta ctctctaaga
     8641 aggcaaatgc ttctttcata actaaatgga gtaaaaacaa aaaatatctt caagatccaa
     8701 aagttttact acaagcaata caagaatatg aaaaactaga agaaacatat ggacaatgcc
     8761 aaaaaccact ctactaccta tccttgcgat catatttgaa ccaaaaagat tcagtgataa
     8821 agggtatgct aaacaaacta tcagaaacag ctacaaagat ggcaaacaat atccaattct
     8881 ttgagttaaa catatctaaa ataccaaaag aaaaacaaaa agaattcctt aattacaaag
     8941 gtcttaacaa ttacaagcac taccttgaaa aactcttcct tgttgggaaa tatctactgt
     9001 cagataacga agagaaaatt ttcaatctca tgtctaaaac atctcactcc aactggacag
     9061 atatggtaga ggaacttcta aacaagcaga aattcactgt aaaagatgaa gatggtaaaa
     9121 acaaagaaat ctcctacaat gaaatctcca aatatctcaa ttctacaaac aagaaagtaa
     9181 gagactatgc ttcaaacgaa tttaacaaaa taaacagtaa atatgcagag atagcagagt
     9241 ttgaaatcaa ttctatttta gaagcgaaga aagtctctga tgattacaga aaaattcctc
     9301 gtccagattt aacgagacat attgcagatg atatagatag cgagacagta gatacattgg
     9361 taaaggtagt aacagataat ttcaacatat ctcaacagta ctacaagaaa aaagcaaaat
     9421 tacttggcct tcagaaattg ggataccatg agagaaatat cccccttaaa ggtatagata
     9481 taccctaccc ttacaagaaa ggaatggcac ttgttaagga aactttttcc tcacttgact
     9541 ctaaatttga agaaattctt aattcttttg aaaaagagaa tatttacgat gtatatccaa
     9601 aagaaagtaa aagtggtgga gcattttgta tctctgttgg tagatccctt cctacatata
     9661 tacttttaaa ctatacggac agattgaacg atgttcttac catagcccac gagagtggtc
     9721 atgggataca ttcaattctc tctaaaaaac agactgaact aaacagtgga cacccaatat
     9781 cccttgcaga ggtagcaagt acattttttg aagattttgt attagaaaaa attctagaga
     9841 agaccaatga caaagaagtt ttattgggaa tcttggataa caagttaaat ggagacatct
     9901 ctagcatatt tagacaaatt gctttttaca attttgagaa agagttgcat gccacataca
     9961 gagagaaagg atttgtacca aaagaagaaa tctcagagat gttttgtaag catatgactg
    10021 catatcttgg agatagtgta gaacaggatg agagtatgag aaatggttgg atttatgtta
    10081 gtcattttag aagattcttc tatgtatatt cgtatgctag tggtttgtta atttccaaag
    10141 cgctacagaa aatggtaaag gataatccca agaatatatc ctatgtgaag gcttttcttg
    10201 aatctggtgg aagtaaatct ccaaaggtat tgtttaaaga aatgggtata gatatttcta
    10261 aggaagagat ttggaaaaaa gggataaagg agataaaata tctattagat aaattagatg
    10321 ggatataact atgaatcaag ataattggtg ggaaggcggg tcacaagata gaacagacca
    10381 aacattagag caatattcac aagatgttca agagtatctt gaaacagaag gtattaaagg
    10441 atacctactt ggtgacccaa caatctgtgg gtatacaaca atgcaggtat gtgttttaga
    10501 tccacaagaa gatatttata ataatgtttt actattcgct acacaaataa ctaaatttaa
    10561 tgatccagag tttaaacttc aaccattaaa gaaagatagt tataattata tttacgataa
    10621 tttccacaag gattatcagc ttgaattaca atctgaggga gtagataaca aaatacaatc
    10681 ttttcaagga agattcgata gaaaagctaa aattacagag atgcgtgaaa tataaattgc
    10741 cggacctaga ttcgaactag aattaacggt tccagagacc gtcgtcc
//
LOCUS       DAOQWP010000022         9975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_7257_length_9975_cov_7.401915, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000022 DAOQWP010000000
VERSION     DAOQWP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7257_length_9975_cov_7.401915"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /gene="rpoA"
                     /locus_tag="PKI16_03005"
     CDS             complement(<1..802)
                     /gene="rpoA"
                     /locus_tag="PKI16_03005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNR53418.1"
                     /translation="MEAFTDIKVVIKEEKENFGLFEISPLPRGYGNTLGNGLRRILYS
                     SLKGTGITQVKIAGVEHEYSTIEGVKEDMVDVILNLKQVKFKTNLDEVFTCKISAKGK
                     KEVKASDIEISGDLEVVNKDLHIATLTDASSKLDMELTVESGIGYKSVDENARKEVGL
                     IPLDCDFSPIEKISLSVEQARKGQETDLDAVLIGVTTDGSITPKDALIESAKIMQEFA
                     GKVMIAMGISKSEVEILAEEANKIEEVQEEVTIINDEIGSWKVEDLPIS"
     gene            complement(820..1431)
                     /gene="rpsD"
                     /locus_tag="PKI16_03010"
     CDS             complement(820..1431)
                     /gene="rpsD"
                     /locus_tag="PKI16_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNR53419.1"
                     /translation="MGRYTGPKERLSRREGVNLHLKGERSFSDKNGLQRKPFVPGQHG
                     SKRRTRLSNYGIQLREKQKIKRTYGLREKQMKSVFKEADRRSKTRNSDKGLELLRILE
                     LRLDNVLYLAGLAHSRNSARQAVVHGHVKLNDSKCRIPSLELKEGDSVELKLAKLAPS
                     KKFFATPKWIETKDSKCKVANLPTREDIDEGIKENLVIEFYSR"
     gene            complement(1457..1843)
                     /gene="rpsK"
                     /locus_tag="PKI16_03015"
     CDS             complement(1457..1843)
                     /gene="rpsK"
                     /locus_tag="PKI16_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNR53420.1"
                     /translation="MAKVKTGKKTKKVVTSGMAAIQSTFNNTIITLTDENGEVLGQGS
                     PSMVGFKGARRSTAFAATKAAMEVADRVIKKFGLKEVKVFIKGPGAGRNSAVKGLDSA
                     GLKVTMLVDRTPIPHNGCRARKLPRK"
     gene            complement(1860..2243)
                     /gene="rpsM"
                     /locus_tag="PKI16_03020"
     CDS             complement(1860..2243)
                     /gene="rpsM"
                     /locus_tag="PKI16_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNR53421.1"
                     /translation="MARIAGIEIENEKKLWVALLGIYGIGEASAKEMCKKTKIDENTK
                     IKDLTEVQLDVIRKYIEENFVVEGELRQNIFRDIKRLKDIRCYRGIRHKLGLPVRGQR
                     TRHNARTRKGRGRAVGGLKRVLEKT"
     gene            complement(2263..2379)
                     /gene="rpmJ"
                     /locus_tag="PKI16_03025"
     CDS             complement(2263..2379)
                     /gene="rpmJ"
                     /locus_tag="PKI16_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNR53422.1"
                     /translation="MKVQASVKKRCPDCYSVKRGRRVFVYCKKNPKHKQRQG"
     gene            complement(2381..2614)
                     /gene="infA"
                     /locus_tag="PKI16_03030"
     CDS             complement(2381..2614)
                     /gene="infA"
                     /locus_tag="PKI16_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010199824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNR53423.1"
                     /translation="MIDSKKVFEFEGVVKESLPNTQFVVEIDVNGEKHNVIGYLSGKM
                     RMHYIRILEGDKVKIEMTPYDKEKGRITYRYMN"
     gene            complement(2742..3389)
                     /locus_tag="PKI16_03035"
     CDS             complement(2742..3389)
                     /locus_tag="PKI16_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HNR53424.1"
                     /translation="MKTILFHGPSGSGKDTQVELLVDKFEIEKIGTGEMFRAMYSEGD
                     IDAIKAYEYWHKGKFVPNDLTYKMFSKWLTKFNQEKDWILVSVVRDIGQVPLLDELLA
                     QYGRKLDVFLHLKLSEEMAIERRTLRWTCPRCGSTYHKKYKKEKVEGYCDKCGMKLSQ
                     REDDTLERTKSLYSEYQKTITPILAEYSSRGIVKEIDASPGIEEIHQEIIKQLNL"
     gene            complement(4043..5308)
                     /gene="secY"
                     /locus_tag="PKI16_03040"
     CDS             complement(4043..5308)
                     /gene="secY"
                     /locus_tag="PKI16_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNR53425.1"
                     /translation="MTNIKDRIKSIFKTKEIRNKIIFSLLILLIYRILSAVPISGIPA
                     DALVELFKGTQFGDILSTVSGGVLESATIVAIGLTPYINASIIFQLLGTVIPKLKELR
                     KEGQDGRRKISMYTRLATVPLAILQSFVIYSTLRGFGLVGTLSPMELAVMAATLTAGA
                     VIMMWMGELISEDGIGGGTSYLIMLGILAGIPGTIRTNFLTMDPLQKTFFIGISVLLI
                     VATIVITQTERRIPIQYSRRVRAGGSMESYIPIKLTQSGVMPVIFAMSLFSFPQLIGK
                     FLVSKDYSENITMWGNKILEMMNNQYIYNISVFVLIIAFSFFYLTVVFNTDELAENLQ
                     KQGAFIQGIRPGNETSEYLRKVSFRLAAVGSLFLALVSILPNIFINTGFITATVISGT
                     GLLIVVGVVLDMKRQIESMMIVRSYDKYL"
     gene            complement(5319..5765)
                     /gene="rplO"
                     /locus_tag="PKI16_03045"
     CDS             complement(5319..5765)
                     /gene="rplO"
                     /locus_tag="PKI16_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNR53426.1"
                     /translation="MKLETMVKRKARVMKGKRIGRGYGSGVGGHTVGRGAKGQKSRSG
                     HKSLISFEGGNVPFHRRMPKYKGFNNKFKVENAAVNVFVLEENYKDGETVTVESLKEK
                     GIVRKNAIGVKILGNGDLKKKLVIEGITLVSSSAREKIEKAGGSIK"
     gene            complement(5765..6370)
                     /locus_tag="PKI16_03050"
     CDS             complement(5765..6370)
                     /locus_tag="PKI16_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNR53427.1"
                     /translation="MMDDKQTYNRGERKEFDKKSLPIRRVAKVTKGGKRLRFSTVVVV
                     GDHNGKVGVGLGRGVDVRSAVEKGERIATKAMKRIELIGDTIPHEITYKYGAAQVLLR
                     PARTGTGVIAGSSVRTVLELAGVDSVYGKILGTQEPNANAYCTFEALKALRNGRVLKK
                     MSKMRERIEVKKEIDEEKKKKMAKRKSKEKKTDRNKVSRRR"
     gene            complement(6378..6701)
                     /locus_tag="PKI16_03055"
     CDS             complement(6378..6701)
                     /locus_tag="PKI16_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004275673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNR53428.1"
                     /translation="MKNNKDQKRQRRKLHIRKNLSGTSTKPRIFVFKSNRYFYAGLAD
                     DDKNIVIKSLMCKRKSDEIKKMAKDFAKIASKFDSAVFDRSGYRYHGLIALFAEELRA
                     NGIKI"
     gene            complement(6704..7246)
                     /gene="rplF"
                     /locus_tag="PKI16_03060"
     CDS             complement(6704..7246)
                     /gene="rplF"
                     /locus_tag="PKI16_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNR53429.1"
                     /translation="MSRIGLEPIKIEEGINVTINGQEIVVASPKAEMKITLPKEITVE
                     IKDNMINLVRKDEAKQSKSNHGTYRMLVANAIEGLKNGFTKTLELVGVGYRARMEGEV
                     LVMSLGWNHPVKVQPYEGIEIEVPNETKIIIKGYNKQKVGEMAAKIRGIRKPEPYKGK
                     GIRYEGEYVRRKSSKSSVAA"
     gene            complement(7249..7626)
                     /gene="rpsH"
                     /locus_tag="PKI16_03065"
     CDS             complement(7249..7626)
                     /gene="rpsH"
                     /locus_tag="PKI16_03065"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNR53430.1"
                     /translation="MNDLIADLLARIQNGIMRKKETVEVVNTKINKGILDVLKMEEMI
                     KDYVDNGRMLEVEIMYDGLEPVISHFERVSKPGQRVYVTKKEIVPIMNGRGISIISTP
                     KGLMSGAMAKSKNLGGELICKIW"
     gene            complement(7644..7838)
                     /locus_tag="PKI16_03070"
     CDS             complement(7644..7838)
                     /locus_tag="PKI16_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008685830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNR53431.1"
                     /translation="MSTEAKEKKARDLKISSKFSTRVYNRCELCGRSRGYLRHYGICR
                     ICFRNLASQGKIPGVKKAIW"
     gene            complement(7822..8376)
                     /gene="rplE"
                     /locus_tag="PKI16_03075"
     CDS             complement(7822..8376)
                     /gene="rplE"
                     /locus_tag="PKI16_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNR53432.1"
                     /translation="MARLKEEYNNKIKQELMKEGGFKNPMEVPMLKKIVVNVGAGEAV
                     NNKTAIEEIVEILRLITGQKPVVNKAKKAVSAFKIREGMEIGVSATLRGDRMWEFFDK
                     LVNVVLPRTKDFRGLSPKSFDGAGNYSLGVEDHTVFVEIDPNNVTKIRSLEITIVTTA
                     KNDKDARLLLDKFGFPFKKDVNRS"
     gene            complement(8493..8798)
                     /gene="rplX"
                     /locus_tag="PKI16_03080"
                     /pseudo
     CDS             complement(8493..8798)
                     /gene="rplX"
                     /locus_tag="PKI16_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
     gene            complement(8814..9185)
                     /gene="rplN"
                     /locus_tag="PKI16_03085"
     CDS             complement(8814..9185)
                     /gene="rplN"
                     /locus_tag="PKI16_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNR53433.1"
                     /translation="MIQRQTKITIADNSGAKVAKVIGIPGSSKRRYAYVGDVVNVAVQ
                     KATPNAGIKRHDVDKAVIVRTHKEYRRKDGTYIRFDDNAGVLVDPKNKTPKGTRIFGP
                     VARELKEKGFDKIVSLAPEVL"
     gene            complement(9166..9438)
                     /locus_tag="PKI16_03090"
     CDS             complement(9166..9438)
                     /locus_tag="PKI16_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uS17 family ribosomal protein"
                     /protein_id="HNR53434.1"
                     /translation="MENNINKKGIKRELHGEVVSNKMTKTVRVKVGSVVRHPLYQKVQ
                     TRNKIFFAHTEKDLNIGDKVVIRESKPYSKNVKWVVVEKNDDTKTN"
     gene            complement(9495..9908)
                     /gene="rplP"
                     /locus_tag="PKI16_03095"
     CDS             complement(9495..9908)
                     /gene="rplP"
                     /locus_tag="PKI16_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNR53435.1"
                     /translation="MLEPRKRKYRKEFRGKMGGVASSNNEVVFGEYGLKAMENGWINA
                     REIEAARRAITNFTKRKGKVWIKIFPHKPYTQKSTNSKMIGGKGAVEGYVAVVVPGTI
                     LFEIGGVDEKVAREALGVGAQKLSIKVKFVQKRLI"
BASE COUNT         2660 a         2139 c         1226 g         3950 t
ORIGIN      
        1 tagatattgg taaatcttct actttccagc tgccaatttc atcattaata attgtaactt
       61 cttcctgaac ttcttctatt ttatttgctt cctctgcaag aatttcaact tcgcttttag
      121 agattcccat tgcaatcatt acttttccag caaattcttg cattattttt gcagattcaa
      181 ttaaagcatc ttttggagta atactgccat cagttgttac accaattaac acagcatcca
      241 aatctgtttc ttgtcctttt cttgcttgtt ctacacttaa actaattttc tcaattggag
      301 agaaatcaca gtcaagaggt attagtccta cttcttttct tgcattctca tctacgctct
      361 tgtatccaat tcctgattca actgttaatt ccatatccaa cttggaagat gcatctgtaa
      421 gtgtggcaat atgtaaatct ttgtttacta cctcaagatc tcctgaaatt tcaatgtctg
      481 aagctttaac ttctttcttc ccctttgcag agattttaca agtaaagact tcatccaaat
      541 ttgttttgaa ttttacctgc tttaagttca agattacatc aaccatatct tcttttacac
      601 cttcgattgt cgagtactca tgctctacac cagcaatctt aacctgagta attcctgttc
      661 ctttaaggct agagtaaaga attctcctta atccattgcc tagtgtattc ccgtatcctc
      721 ttggaagagg ggaaatctca aagagtccaa aattttcttt ctcttctttt attactacct
      781 ttatatctgt aaaagcttcc atgtattttt tattaaaaat tatcttgagt agaactcaat
      841 tacaaggttt tccttaatac cttcatctat atcttctcta gttggtaaat tcgcaacttt
      901 acattttgaa tcctttgttt ctatccattt aggtgtagca aagaatttct ttgaaggtgc
      961 aagtttagcc aattttaatt caacactatc tccttctttt agctcaagag atgggattct
     1021 acatttagaa tcgttcaatt ttacatgtcc atgaacaact gcttgtcttg cagagtttct
     1081 tgaatgtgca agtcctgcta gatacagtac attatccaat cttagttcca atattcttag
     1141 aagctctaat cctttatctg agtttctagt ttttgatctt ctgtctgcct ccttaaagac
     1201 agatttcatt tgtttctctc ttaatccata tgttctttta attttctgct tttctctaag
     1261 ttgaataccg tagttagata atctagttct tcttttactt ccatgctgac caggaacaaa
     1321 tggttttctt tgtaatccat tcttgtctga aaaggatctc tcacctttta ggtgaaggtt
     1381 tactccttct cttctactta atctttcttt tggtcccgta tatctaccca tttaattagt
     1441 taatcgtaat atattattat tttcttggca gttttcttgc tctacaacca ttgtgtggaa
     1501 taggggttct gtctaccaac atcgttactt tgagcccagc gctatctagt ccttttacag
     1561 cagaatttct acctgctcca ggtcctttga taaatacttt aacttctttc aatccaaatt
     1621 tctttatcac tctatctgca acttccatag ctgcctttgt tgctgcaaac gcagtgcttc
     1681 ttcttgctcc tttgaatcca accatacttg gagatccctg tcccaaaact tctccattct
     1741 catctgtaag tgtaatgatt gtgttattaa aagtggattg tattgctgcc attccacttg
     1801 taacaacttt cttcgttttt ttacctgtct ttacttttgc catttaatta atagttatgt
     1861 tatgtttttt ctaatactct cttaagtcca cctactgctc ttcctctacc ttttctcgtt
     1921 ctagcattat gtctagttct ttgacctctt actggtaatc caagcttatg tctgattcct
     1981 ctgtaacatc tgatatcttt taatcttttt atatctctga agatattctg tcttaactct
     2041 ccttcaacta caaagttctc ttctatatat tttctaatta catctaactg tacctcagta
     2101 agatctttta tttttgtatt ctcatctatc ttagtcttct tacacatttc cttagcacta
     2161 gcttctccta tcccgtatat accgagcaat gctacccaga gtttcttttc attttctatt
     2221 tctattcccg caattcttgc cattaaatat aattaaaaaa tattaacctt gtctttgttt
     2281 atgtttagga ttctttttgc aatatacaaa aaccctccta cctcttttta cagaataaca
     2341 atcaggacat ctttttttta cagaagcttg tactttcatt ttaattcata tatctatatg
     2401 ttattcttcc cttttcttta tcataaggag tcatctcaat tttgacctta tctccctcaa
     2461 gaattcgaat gtaatgcatt ctcatcttgc ctgatagata ccctatcaca ttatgctttt
     2521 ctccgtttac atctatctca acaacaaatt gagtgtttgg caaactttct tttaccactc
     2581 cctcaaattc gaaaactttt ttactatcaa tcatttttcg ctattttatg taaacagact
     2641 cccaagcatt atatcaaagt agcgcctttg tttcaacaaa atcgtgaaag aatgcgttac
     2701 ctgaaaggga acaggaaaag aatatcaaaa aagaggacta tttacaagtt taattgctta
     2761 attatctctt gatgaatctc ctcaattcct ggcgaagcat caatctcttt tactatgcct
     2821 cggctagaat attcggcaag tatgggagtt attgttttct gatactcact atacaagctc
     2881 tttgttcttt caagagtatc atcttctctt tgagataatt tcataccaca tttgtcacaa
     2941 tagccttcca ctttctcctt tttgtacttt ttgtggtatg tagagccaca tcttggacat
     3001 gtccatcgta aagttcttct ctcaatagcc atttcttctg agagctttag atgtaaaaac
     3061 acatccaatt ttctgccata ctgtgcgagt aattcatcta gcaatggaac ttgtccaata
     3121 tctcttacaa cagaaactaa aatccaatct ttctcttgat taaatttcgt taaccatttt
     3181 gaaaacatct tgtatgttaa atcgttaggg acaaattttc ccttatgcca atattcatac
     3241 gccttgattg catctatatc tccttctgag tacatagctc taaacatctc tcctgttcca
     3301 atcttctcta tttcgaattt gtcgacaaga agttcaacct gggtatcttt tcctgatcca
     3361 gacgggccgt gaaagagtat cgttttcata gtttaattcg ctctagatta tcaaagaata
     3421 taatagcaca aatatataac atcagaaaag aatctattat ttaatatact attttcaaat
     3481 tcatcaattg ttcaaaagca cacattagaa taagatttat gcgatatttc cagaaaaatt
     3541 gtatttatca tatttataac cgaggaaatc ggaagctaaa aatatgttta gatccagaag
     3601 attatacaaa aattatttat ttaataaaaa gatttttccc cacacaaagt tttgatatcc
     3661 tcggatactg tattatgcct aatcattttc atctcttaat agttaaggtt aaaaatcaga
     3721 aaatatcata ttctatgcag atgctagggt ctgcttacac gagatacttt aataagaagt
     3781 atggccttgt tggacatttg ttccaaggta cttataaata tagaaatgtc acaacagaga
     3841 gacaattgtt atcaacttca caatatattt acaaaaatcc agaaagatta tcgaccagaa
     3901 ggtatccttg ggtacatgta gatagaaagt tagtagatgc gtatgctctc attctttttc
     3961 agatgaactc aacccctata gcttattaac tcactatcag gagtatacca gtacaatcgt
     4021 tctacaggga taatttactt acttaaagat atttgtcgta gcttcttaca atcatcatgg
     4081 attcaatctg tctcttcata tcaagaacaa ccccaacaac gattaacaat cccgttcccg
     4141 agattacggt tgcagttatg aatccagtat ttatgaaaat gtttggcaag attgatacca
     4201 aagccaagaa aagagatcct actgctgcca atctaaagct cacctttctt agatattctg
     4261 atgtctcatt tcccggtctg ataccttgaa taaatgctcc ttgtttctgt aaattctcag
     4321 caagttcatc tgtattaaat actaccgtaa ggtaaaagaa agagaaggca ataataagta
     4381 caaaaactga gatattataa atatattggt tattcatcat ctccaagatt ttgtttcccc
     4441 acatagttat attctcagag taatctttgc ttacaagaaa cttacctatt agttgtggga
     4501 aagagaataa agacattgca aagattactg gcatgacacc agactgggta agtttgatag
     4561 ggatataact ttccatagat ccacccgcac gaactcttcg agagtattgt atcgggattc
     4621 ttctttccgt ttgtgtaatt actatcgttg ccactatcaa taacacggag ataccaatga
     4681 agaaagtctt ctgcaaagga tccattgtta agaagtttgt tctaattgtt ccaggaatcc
     4741 ctgcaagaat tccaagcata attaaataag atgttccacc acctattcca tcttctgata
     4801 ttagttctcc catccacatc ataattactg ctccagcggt tagtgtagca gccatgacag
     4861 ctaattccat gggactaagt gttccaacaa gtccaaatcc tcgcaaagtt gaatatatta
     4921 caaaagattg taatatcgcc aaaggtacag ttgcaagtct tgtatacata ctaatttttc
     4981 ttctgccatc ttgcccttct tttcttagct ccttaagttt agggataacc gttcctaaaa
     5041 gttggaatat gattgatgca ttgatatagg gggtaagacc gattgctaca atggttgctg
     5101 attctaaaac accaccagat acagtggaga gaatatctcc aaattgagta cctttaaaaa
     5161 gttctactaa ggcatcggct ggaattccag aaattggcac agcggaaagt atcctatata
     5221 tcagaagtat taaaagagag aagattattt tattacgaat ctcctttgtc ttgaagatag
     5281 actttatacg atctttaata tttgtcattt ttaagaggct attttataga tcctccagct
     5341 ttctcaattt tttctctagc agaggatgac actaaagtaa ttccttctat cacgagcttt
     5401 ttctttaaat ctccatttcc aagaatctta actccgatag cattttttct aacaataccc
     5461 ttttctttca aagattctac tgtgacagtc tccccatctt tgtaattctc ttctaacaca
     5521 aacacattta cagcagcatt ttctacttta aacttgttat taaaaccttt gtattttggc
     5581 attcttctgt ggaaaggtac attgccacct tcgaaagata ttaaagattt atgtccagat
     5641 ctagatttct gacctttagc acctcttcca actgtatggc ctccaacacc actaccatat
     5701 cctcttccaa ttcttttgcc cttcataact ctagcttttc tttttaccat agtttctagc
     5761 ttcattatct tcttctacta actttatttc tatctgtttt tttctcttta ctctttctct
     5821 ttgccatttt cttcttcttc tcttcgtcaa tttctttctt tacttcaatt ctttccctca
     5881 tcttactcat ctttttcaat actcttccat ttcttaatgc tttcaaagct tcaaatgtac
     5941 agtatgcatt tgcatttggt tcttgtgttc caaggatttt cccatagaca ctatctactc
     6001 ctgctaattc taatactgtt cttacagaag atcctgcaat tacaccagta ccagttctag
     6061 ctggtcttag aagaacctga gctgcaccat atttgtaggt aatctcatgg ggaatagtat
     6121 ctcctattaa ttcgattctt ttcattgctt tagttgctat tctctctcct ttttctaccg
     6181 cacttctcac atctacacct ctaccaagtc ctactccaac ttttccatta tgatccccta
     6241 caacaactac tgtgctgaat cttagtcttt ttccaccctt tgtaactttc gctactcttc
     6301 tgattggaag agacttcttg tcgaattctt ttctttctcc tctattgtat gtttgtttat
     6361 cgtccatcat ttaatttcta aattttaatt ccgtttgccc ttaattcctc tgcaaagagg
     6421 gcaattaatc catgatatct gtagccactt ctgtcaaaga ctgcgctatc gaatttactt
     6481 gcaatcttag caaaatcttt tgccattttc tttatctcat cactttttct cttacacatc
     6541 aaagatttaa taacaatatt cttatcatca tccgctaatc cagcatagaa atatcggttg
     6601 cttttaaata caaaaatcct tggttttgta gaagttccac ttagattctt tctaatatgt
     6661 aattttcttc tttgtctttt ttggtcttta ttatttttca taattatgct gctacactag
     6721 atttagaact ttttcttctt acatattcac cttcgtatct tataccttta cctttgtaag
     6781 gttctggctt tctaattcct cttatctttg ctgccatttc tccaactttc tgtttgttat
     6841 atccttttat tattatcttt gtttcgtttg gcacttctat ctcaattcct tcgtatggtt
     6901 gaactttaac tggatgattc catccaaggc tcataaccaa aacttctcct tccattcttg
     6961 ctctgtatcc tacccctact agttccaatg tttttgtaaa tccattcttc aatccttcga
     7021 ttgcatttgc tactaacatt ctgtatgttc catggttaga tttagattgc ttagcttcat
     7081 cttttctaac aaggttaatc atgttatcct taatctctac tgtgatttcc tttggcaatg
     7141 taatcttcat ctcagctttt ggactagcga caacaatttc ctgtccattg atggtaacat
     7201 ttataccttc ttcaattttt attggctcta atcctattct agacattact accaaatttt
     7261 acatattaat tcgccaccaa gatttttact cttagccatt gctccagaca ttagtccctt
     7321 tggagtacta attatggata ttcctctacc attcattatc gggacaatct cttttttggt
     7381 aacatatact ctctgtcctg gtttgcttac tctttcgaag tgagatataa caggctctaa
     7441 gccatcatac ataatttcta cttctaacat tcttccatta tctacataat ctttaatcat
     7501 ttcttccatt ttcaatacat ccaagatccc tttgttgatt ttggtattta caacctcgac
     7561 agtttccttc tttctcatta ttccattttg tattcttgcc aatagatcgg ctattaaatc
     7621 gttcattata tatattcttt aaattaccaa atagcttttt taacacctgg aatctttcct
     7681 tgacttgcta aatttctaaa gcaaatccta cagattccgt agtgtcttaa ataacctcta
     7741 gatctaccac atagttcaca tctattgtaa actcgtgtgc taaacttaga gcttattttc
     7801 aaatctcttg cttttttctc tttagcttct gttgacatct ttcttaaaag ggaatccaaa
     7861 tttatctaaa agtaatctcg catctttatc attctttgct gtagtaacga ttgtaatttc
     7921 caaacttctt atcttagtaa cattgttagg gtcaatctct acaaatactg tgtgatcttc
     7981 aactcctagt gaatagttgc ctgcaccatc aaaggactta gggcttaatc ctctaaaatc
     8041 ttttgtcctt ggtaaaacaa cattaactag tttatcaaag aactcccaca ttctatcacc
     8101 tcttagagtt gcagatactc ctatttccat accttctctt attttaaaag cagaaactgc
     8161 cttttttgct ttattaacaa ctggtttctg ccctgtaatt aatctcaaaa tttctacaat
     8221 ctcctctatt gctgttttat tgtttactgc ttctcctgct cctacattta caactatctt
     8281 tttaagcata ggaacctcca tagggttttt gaatccacct tctttcatca attcctgttt
     8341 tattttattg ttatactctt cttttaatct tgccattttt actttttagt agttttttta
     8401 acactctttt ttacagtttt cttaatttct tttttagtag ccttatcttt tgctggttct
     8461 tttttaactt cttctttctc tattttcttt gcttctatat ctttaccaca ttttttacat
     8521 actcttacaa caccattatc ttccctcctc aagcttactc ttgttgcttt attacacata
     8581 ggacatacta gcattacctt agagactggg agaggcttct cgatacttaa aatctcagaa
     8641 gtccttttct taccatctcc ttttaaatgt cttctgtaaa tgttcaatcc ttctactaca
     8701 accaatcttc ttgttaaatc tacagctact acacgaccag ttttatcgct atcttttccg
     8761 tataatattt ttactgtatc tcctttttta atcttcatat tactatcttt taattacaaa
     8821 acttctggtg caagagaaac tattttatcg aaacctttct cttttaactc tcttgctact
     8881 ggtccaaata ttcttgttcc ttttggtgtt ttatttttag ggtctaccaa tactcctgca
     8941 ttatcatcaa atctaatata tgtcccatct tttcttctat attccttatg tgttcttaca
     9001 attactgctt tatctacatc gtgtcttttt attcctgcat ttggagttgc tttctgtact
     9061 gctacattta ctacatctcc cacatatgca taccttcttt tggaagatcc tgggatacca
     9121 ataactttgg ctactttggc tccactatta tctgcaattg ttattttagt ttgtctttgt
     9181 atcatcgttt ttttctacta caacccattt aacattttta gaatatggct tactttctct
     9241 tatgacaacc ttatccccta tatttaaatc tttttcagta tgtgcaaaga agattttatt
     9301 tcttgtttgt actttctgat aaagaggatg tctaactaca gagcctactt ttactcttac
     9361 cgttttagtc attttgttac tgacgacttc tccatgtaat tctcttttta ttcctttttt
     9421 gttaatattg ttttccatat ttttatttaa ataacggcct gtattctatc aattattact
     9481 cttattctca agctttatat taatctcttc tgtacaaatt taacctttat cgaaagcttc
     9541 tgtgctccaa ctcccaaagc ttctcttgct actttctcat ctactccacc tatttcaaaa
     9601 agtattgttc ctgggactac aactgctaca tatccctcaa ctgcaccttt tccaccaatc
     9661 attttactgt ttgtagattt ttgggtgtaa ggtttgtgtg gaaatatttt tatccatact
     9721 ttaccttttc tctttgtaaa gtttgttatt gctcttcttg cagcttcaat ttctctagca
     9781 ttaatccaac cattttccat ggctttaagc ccatactctc caaagacaac ttcattattg
     9841 gaagatgcta ctcctcccat tttgcctctg aactcttttc tgtattttct tttacgtggc
     9901 tctaacatgg ttctttatat taatatatta ttcttcagtt gctacttcag ttttatttcc
     9961 tttgtatatc catac
//
LOCUS       DAOQWP010000023         8419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_8921_length_8419_cov_7.519010, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000023 DAOQWP010000000
VERSION     DAOQWP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8921_length_8419_cov_7.519010"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /gene="ybeY"
                     /locus_tag="PKI16_03100"
     CDS             complement(<1..382)
                     /gene="ybeY"
                     /locus_tag="PKI16_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNR53436.1"
                     /translation="MKLEIFNWPNISIQETIVINSLQKEFSSFDISTINVVFVDEKEM
                     QRLNEEYRNINDVTDVLSFNLNAEGFLGEIYICPQYIKKTVEKYQEELLRVIIHGILH
                     LLGYEHKTKLDDISSQKEEMFVKQE"
     gene            complement(379..825)
                     /locus_tag="PKI16_03105"
     CDS             complement(379..825)
                     /locus_tag="PKI16_03105"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNR53437.1"
                     /translation="MSLTGDLRKDMFTFSKEGNTNASDILKMALASIKNAQIAQESEM
                     TQQDEEKIIRKEVKKIEDSIAQFTLMGRLDLVEKERSQLEILQKYLPEQMSEEDIKAI
                     INSKIKELNISSMNDMGKLMGAVMKETAGKADGNTVKNLVTQILTK"
     gene            complement(849..1052)
                     /gene="rpsU"
                     /locus_tag="PKI16_03110"
     CDS             complement(849..1052)
                     /gene="rpsU"
                     /locus_tag="PKI16_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNR53438.1"
                     /translation="MSVIVHSNENIDSALKRLHREVLREKILETYREKAYRVKASLGD
                     IAKRKEWAKMKRRRRTAARRAKN"
     gene            complement(1100..>1761)
                     /locus_tag="PKI16_03115"
     CDS             complement(1100..>1761)
                     /locus_tag="PKI16_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53439.1"
                     /translation="DKKGATSTSCALTTTFSILAEEQNPSWDITKKGAEQCLESGDAQ
                     IIYTIKVKNIGTGTGSIDKIVDALDSKVLASYITGTISGGGLFNSGNITWELEAEEET
                     FAADQEKTYTYTLVIPKTAFGSYTNMVTATPTTGENFSDSVEVKVDCIEDIPQTGLFD
                     STVIKIVAGIILLLLGFNLHRIDVSFRKVQDNLHKLSVSIQDTQDERRKRNFEKKIVK
                     K"
     assembly_gap    1762..1861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1862..2893)
                     /locus_tag="PKI16_03120"
     CDS             complement(<1862..2893)
                     /locus_tag="PKI16_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53440.1"
                     /translation="MAIRLKLTKKWKIIVIIFFVLILGGSGGYLLWRTNQPETVAPTD
                     SEATGGAGACCNPKYGCVAGWKCKTGGCNETLCVDEETCPFYKGIQGTKTGKCENDPW
                     VGKTKCDYRFAGTCVRETDPGGPSEGDCKDTKCTWPKVLMSDNNCACELCNGSNGCSG
                     NPPTSCTLKDCPSGYENCGTTVSHEDSGCTQRSDISCTVYHRDCNNPAVIYRYCKKEG
                     STTTNTCDSGEWLTKPTGSYKYCDPITYTAKAKDSDGIDKTTVVAKLNNSNRSTVSFT
                     NETTTAVDISESLSTTTNCLPVGTYTLDLSWKDKKGATSTSCALTTTFSITTNTCDSG
                     EWLTKPTGSY"
     gene            complement(2895..4037)
                     /locus_tag="PKI16_03125"
     CDS             complement(2895..4037)
                     /locus_tag="PKI16_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNR53441.1"
                     /translation="MLSIILNVLLFVLVIGVLTFVHELGHFLVAKAIKAKVYEFALGF
                     GPKLLKKKYKGTEYSIRMLPLGGFVKIMGDGDPGNNKEIKDDPGNLNNKPKIQQVVVM
                     LAGVTMNILFCVVVYFIVIASMGWKIPLSNDFEGFNPVGGTISREIVGGVEYSEVVDG
                     GNAQKAKIPAQGEIKSIGGVELTYSDEIKDLLAKSKGESVNINICEEDVCKDYLVDVS
                     DKGTIGISLGSNYYVILSYENNKAFSGFAHVINTLFLISDRLGDMFKEAKQTGDYSDL
                     SNSVSGPVGIYFLIDYFKQFGVITFLSVMADLSLSLAIINILPIPALDGGRVLILMIE
                     GISRKDLNEKVESLIINTSFILLMILVLFIMIKDIVNIDNIKDMFS"
     gene            complement(4054..4599)
                     /gene="frr"
                     /locus_tag="PKI16_03130"
     CDS             complement(4054..4599)
                     /gene="frr"
                     /locus_tag="PKI16_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNR53442.1"
                     /translation="MDIDLFKVDIQKCLDHLNDELSQIRTGRATPELVQEIPVNAYET
                     MAPIKNYANINVMDAKTIAIQPWDKSIVDNISKAVTNANMGFLPSRDGDRILVKIPDL
                     TEERRKEYVKVMKERVEDGRVAVRQVRQKYMKELDDMQASGLSEDEADRAREEIEKVV
                     KEINTKIEEIREEKEKDLLTI"
     gene            complement(4671..5384)
                     /gene="tsf"
                     /locus_tag="PKI16_03135"
     CDS             complement(4671..5384)
                     /gene="tsf"
                     /locus_tag="PKI16_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNR53443.1"
                     /translation="MNITVDDIRSLRNKTSAGMGLCKEALTKSEGDMAKAIEYINERS
                     DVISRLRNLTGAKIGLCKLAYDEAEKDFEKAVTLINERGWNEPIGDEEVEIGEGIIES
                     YVHGREQRLVALVEVKCKTDFVARNEDFKAFAHELALQIAAMKPEYISKESISEEELT
                     KLKDIFQKEAESEGKPENIREKIVEGKLSKFYSEKCLLEQKWFKDETKTISNLLDEAT
                     QKLGEPLEIRRMLLWEFGK"
     gene            complement(5403..6536)
                     /gene="rpsB"
                     /locus_tag="PKI16_03140"
     CDS             complement(5403..6536)
                     /gene="rpsB"
                     /locus_tag="PKI16_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNR53444.1"
                     /translation="MEKTTKKVVEETKTLNKVSTVKLPELIELLQAGCHFGHKKSGWN
                     PRMKQYIYEERNGIHIIDLVKTQELLKKAVEELEKLSNTGNILIVGTKGQAASIVQNI
                     AEEKGAFYVNRRWPGGLFTNFETIRRSIQNLVKMEEEVAQGGKDLVKKEVLMMEKEIA
                     KLNMIYKGIKFMDKLPAAIIVIDSKVEKIAVREANRVGIPIISMIDTNSNPDLITYSI
                     PANDDSLKSITLIMNVLGEVIGTSKYGNSLVALRRDHEAVLDRMNRNFLEKKEREEKM
                     ANEERERMKALREGKIAESKGTVVRVVKKDITADIVAAEEAKKEADSRQIEDLGLSAR
                     VLKALKEGGIKTISDLSGKSEKDLENIKGIGEKAAKEILKSLR"
     gene            complement(6661..7293)
                     /gene="radC"
                     /locus_tag="PKI16_03145"
     CDS             complement(6661..7293)
                     /gene="radC"
                     /locus_tag="PKI16_03145"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNR53445.1"
                     /translation="MLPREKYLERGIDSLSDTELVAILVGSGVKGKDFFSISRSIVKR
                     IRKVVSNGKDICIEDIDGVEGVGIVTAMRILAGMELGRRVYGLCKSERSLVRDSEDAY
                     GLLRDIGSKKQEYVVGLFLNSRFEVLDKRVVGMGSLDIVSVLPRDIITPALELNAALV
                     VIAHNHPSGDPSPSEEDILFTKRLSEALDLVGIKLLDHIVIGGETWKSIS"
     gene            complement(7339..8178)
                     /locus_tag="PKI16_03150"
     CDS             complement(7339..8178)
                     /locus_tag="PKI16_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HNR53446.1"
                     /translation="MVDICYSQNFYKNANHLKEMVKNASFSSDDIVLDIGAGKGVIAQ
                     ELSKYSDNVTAFELDTKYFRELTKNLSSLPIKLINDDFLLTNLPTKDFKIFSNIPFAL
                     TSEIINKITAIDSSLIEAFLFVQEEAADRFLGEPFNTQIATILSSRYTFSIVEELDRY
                     DFSPVPSVDIVLLKIVKRENPEVEFELYRDFVSYIFNQTNRFVIDTLKKIFTEKQMKY
                     IKKYLLEKGYNTPSDIPRRYYLEIFQYFKMNGDNYKKKVRGYYQKHTEQHSKREKVNR
                     TRI"
     gene            complement(8186..>8419)
                     /locus_tag="PKI16_03155"
     CDS             complement(8186..>8419)
                     /locus_tag="PKI16_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HNR53447.1"
                     /translation="SKLLLKNSKYGKFWACEKYPECKGTLPLLLQEKCPECGGNLVER
                     RSKWGKTFIGCSTYPKCKYIKKSFKAKKKKKVV"
BASE COUNT         2190 a         1737 c         1223 g         3169 t
ORIGIN      
        1 tctcctgttt tacaaacatc tcttcttttt ggctagatat atcatctagt tttgtcttgt
       61 gttcatatcc aagtagatga agaatgccgt gtatgataac tctgagaagt tcttcttgat
      121 acttttctac tgtctttttt atatattggg ggcatatgta tatttctcca agaaatccct
      181 ctgcattaag attaaaagat aacacatctg taacatcgtt gatattgcgg tactcttcat
      241 ttagtctttg catctccttc tcatctacaa aaacgacatt gattgtagat atgtcaaagg
      301 aagaaaattc tttctgaaga gaattaataa cgatagtttc ttgtatggat atatttggcc
      361 agttgaatat ctctaatttc atttggttag tatttgggta acaagatttt ttactgtatt
      421 tccatctgct ttaccagcgg tttctttcat tacagctccc attagcttac ccatatcgtt
      481 catgcttgag atgtttaatt ctttaatctt gctgtttatg attgccttta tatcttcctc
      541 tgacatttgt tcagggagat atttttgtaa gatttctaat tgagatcttt ctttttctac
      601 aagatccagt ctacccatta gtgtaaattg agcgatggaa tcttcaatct tttttacttc
      661 ttttctaatg attttttctt catcttgctg tgtcatttca ctttcttgag caatttgagc
      721 attcttaatt gatgctaaag ccatttttaa tatatcacta gcattggtat ttccctcctt
      781 agagaaggta aacatatctt ttcttagatc acctgtgaga gacatttgta aaagcgttaa
      841 aattaagatt aatttttagc tcttcttgct gcagttcttc ttcttctttt catctttgcc
      901 cactcttttc tttttgcaat atctcctaga gaagctttta ctctgtatgc tttttctctg
      961 tatgtttcga gtatcttttc tctaaggact tctctgtgaa gtcttttgag ggcactatct
     1021 atattttcgt tagaatgtac gattactgac atagttttat atatttatta tgtatgctcg
     1081 gtgattatat catacctgtt tactttttta ctatcttttt ctcaaaattt ctcttccttc
     1141 tttcatcttg ggtatcttgt atggatactg aaagcttgtg aagattgtcc tgtacctttc
     1201 tgaagcttac atctattctg tgtaagttga atcctagtag gagcagaatt atacctgcaa
     1261 ctatcttgat tacagtactg tcgaagagtc ctgtttgtgg tatatcttcg atacagtcaa
     1321 ccttaacttc tacactgtcg gagaaattct ctccagtggt tggagttgct gttaccatgt
     1381 ttgtgtaact accaaatgct gtcttaggta tgaccaaagt gtatgtgtat gtcttttctt
     1441 ggtctgcagc aaatgtttcc tcttctgctt ccaattccca tgtgatattt ccactattaa
     1501 agagtcctcc accagagatt gttccagtta tgtaggaggc taatacttta ctatctaaag
     1561 catctactat tttatctatg cttcccgtac ctgtaccgat attttttact ttgatggtat
     1621 atattatttg ggcgtctcca ctttcaaggc actgttctgc tcccttcttc gttatatccc
     1681 agcttggatt ttgttcttct gcaaggatgc tgaaggttgt tgtaagggca cagcttgtac
     1741 ttgtggctcc ttttttgtcc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nataactacc ggttggttta gttaaccact ctccgctatc gcaggtgttg gtggtaatgc
     1921 tgaaggttgt tgtaagggca cagcttgtac ttgtggctcc ttttttgtcc ttccagctta
     1981 ggtctagggt gtatgttcca actggtagac agtttgtggt agtgcttagg gattcggaga
     2041 tgtctacagc agtggtggtt tcattggtga aggatactgt ggatctgttt gagttattta
     2101 gtttggcaac tacggttgtt ttatctatac cgtcactatc tttagctttt gcagtgtatg
     2161 tgattgggtc gcagtattta taactaccgg ttggtttagt taaccactct ccgctatcgc
     2221 aggtgttggt ggtagtagag ccttcttttt tacagtaacg atatatgacc gcagggttgt
     2281 tacagtctct gtggtataca gtacagctga tatctgatcg ttgtgtacat ccagagtctt
     2341 cgtgactaac tgttgttcca caattttcat atccagaggg acagtctttc aaagtacaac
     2401 ttgttggtgg attaccgcta catccattag agccattaca taattcacat gcacaattgt
     2461 tgtcactcat gagtactttt ggccatgtac attttgtatc tttacaatct ccttctgagg
     2521 ggcctcctgg gtccgtttct ctcacacaag tcccagcaaa tctataatca catttggttt
     2581 tccctaccca aggatcgttt tcacacttcc cagttttagt accttgaatg cccttataaa
     2641 agggacaggt ctcttcatct acacaaagtg tctcattaca accaccagtt ttacacttcc
     2701 atcctgctac acaaccatac tttggattgc aacatgcccc tgccccacca gtggcctcac
     2761 tatctgtagg tgccacagtc tctggttgat tggttctcca tagtagatat cccccactac
     2821 ctccaagtat taatacaaag aagataataa ctattatctt ccactttttt gttagtttta
     2881 atctaatagc catactaact gaacatatct tttatattat ctatattaac aatatcctta
     2941 atcattataa atagtacaag aatcattaac aaaataaaac tagtatttat tataagactc
     3001 tcaacctttt cattaagatc tttccttgat ataccctcta tcattagaat taaaactctt
     3061 ccaccatcca acgcaggaat tggaagaata ttaataattg ccaaagacaa agacaaatct
     3121 gccatgacac taaggaaagt tataacccca aattgtttaa agtaatctat taggaaatat
     3181 atacctactg gcccagaaac agaattagaa agatctgaat aatctcctgt ctgtttagcc
     3241 tccttaaaca tatcccctaa tctatctgat atcaaaaaca gagtatttat tacatgagca
     3301 aaaccagaaa atgctttatt attctcatat gacagaatta catagtagtt agaacccaga
     3361 gatataccaa ttgtaccctt atcggagaca tccacgaggt aatctttaca aacatcttct
     3421 tcacagatgt ttatatttac actttcaccc ttactctttg ctaaaagatc ttttatctca
     3481 tctgaatatg taagttctac tccaccaata cttttgattt ctccctgtgc aggtatcttt
     3541 gctttctgtg cattgccacc atctacaact tcagaatatt ctacaccacc aacaatctct
     3601 cttgagattg ttccacctac tggattgaaa ccctcaaaat cgtttgaaag gggaatcttc
     3661 caccccatag atgctataac aatgaaatat actactacac agaaaagtat attcatggta
     3721 acaccagcta acattactac gacttgttgt attttgggtt tgttatttaa gtttcctgga
     3781 tcatctttta tctctttgtt gtttccagga tctccatccc ccattatttt tacaaaacca
     3841 cctagaggaa gcatcctaat actgtattct gttcccttgt atttcttttt taaaagttta
     3901 ggaccaaaac ccaatgcaaa ttcatatacc tttgctttga ttgccttggc tactagaaaa
     3961 tgtccaagtt catggacaaa ggtaagaact ccaattacaa gaacaaacaa gagaacattt
     4021 aggatgatgg atagcatatt taaaatgtac agattatata gttagaagat ctttttcttt
     4081 ctcttctctt atctcctcta ttttcgtatt gatctctttt acaacctttt caatttcttc
     4141 ccttgctctg tctgcctcat cttctgaaag accggaagct tgcatatcat ctaattcctt
     4201 catatatttc tgtcttactt gtcttacagc aactcttcca tcctctactc tttctttcat
     4261 aactttaaca tactcttttc ttctttcttc tgttaagtct ggaatcttta ccaaaattct
     4321 gtctccatcc cttgaaggaa gaaaacccat gttagcgtta gttacagctt ttgaaatgtt
     4381 atctacaata cttttatccc atggctgtat tgcaatagtt ttagcatcca tgacatttat
     4441 gtttgcatag tttttaatgg gagccattgt ctcatatgca tttactggta tttcttgcac
     4501 taattcaggt gtagctctac ctgttctaat ttgagagagt tcatcattaa gatgatctag
     4561 gcatttttgt atatcaactt taaagagatc tatatccata cgaaattata taaatatatt
     4621 atttacaaat tgtatatcaa ctgaggtgta ttgtctagaa agggtcttgg ttatttacca
     4681 aactcccaga gaagcatcct tctgatttca agaggttctc ctaatttttg tgtagcttca
     4741 tctagaaggt tagaaatcgt tttagtctcg tctttaaacc acttctgttc caaaagacat
     4801 ttttcggagt agaatttgct aagtttaccc tccactattt tctctcgaat attctctggt
     4861 tttccttcac tctctgcttc cttttggaat atatccttaa gctttgttag ctcctcttct
     4921 gagatactct ccttggagat atattctggt ttcattgcag ctatctgtag tgctaattca
     4981 tgtgcgaatg ctttaaaatc ttcgtttctt gctacaaagt ctgttttaca cttaacctct
     5041 accaaagcaa ctaatctctg ctctcgccca tgtacatatg attcgattat tccctcacca
     5101 atctctactt cttcgtctcc aattggctca ttccaacctc tttcattgat taaggttact
     5161 gctttttcga aatctttttc tgcttcatca tatgccaact tacaaagacc aatctttgca
     5221 cctgttaagt ttcttaacct gctaattaca tcgcttcttt cattgatata ttctatggct
     5281 tttgccatat ctccctcact tttggtgagt gcttctttac acaatcccat acccgcggag
     5341 gttttatttc ttaagcttct tatatcgtca actgtaatat tcatagcagt ttaatattta
     5401 aattatctta atgattttag aatctctttt gcagcttttt ctcctattcc cttgatattt
     5461 tccaaatctt tttcgctctt tccacttaga tcacttatgg ttttgatacc accctcttta
     5521 agcgctttta gtactcttgc tgagagtcct agatcttcaa tctgtcttga atctgcttct
     5581 ttcttagctt cttctgcagc tacaatgtct gcagttatgt cctttttaac aactctaaca
     5641 actgtacctt tagattctgc tatctttccc tctcttagtg ctttcattct ttccctctct
     5701 tcgtttgcca tcttctcttc tctttctttt ttctcgagga aatttctgtt cattctgtct
     5761 agtactgctt catggtctct tcttagagca acaagagaat tcccatattt ggaggttccg
     5821 attacttctc caagaacatt cataatgagg gtaatactct ttagagagtc atcatttgca
     5881 ggtatggagt atgttatgag gtctggatta ctgtttgtgt caatcattga aatgattgga
     5941 atacctactc tgttagcttc tcttactgca attttttcta ctttactgtc tatgacaatt
     6001 attgctgcag gaagcttgtc catgaactta attcctttgt aaatcatgtt caattttgca
     6061 atttcttttt ccatcatgag gacctctttt ttaacaagat cttttccacc ctgcgcaact
     6121 tcctcttcca tctttacaag gttctggata cttcttctaa tggtttcaaa gtttgtaaaa
     6181 agaccacctg gccatcttct gtttacatag aaagcgcctt tttcttctgc aatattttga
     6241 actattgagg ctgcttgtcc ttttgttcca acgattagga tatttcctgt attagaaagt
     6301 ttctccaatt cttctactgc tttttttagt aattcttgtg ttttaacaag atcaatgatg
     6361 tgaattccat ttctctcttc gtaaatatat tgtttcattc ttggattcca tccacttttc
     6421 ttgtgaccaa aatggcatcc tgcttgaagt agttctatta attctgggag tttaactgta
     6481 gaaactttgt ttagagtctt tgtttcttct acgacttttt ttgttgtttt ttccattatt
     6541 ttttcctttt ataatatctc tggtgggtga tatttatatc atccaggaat tgttcagaga
     6601 tatatgagat atatattaaa cgttaggatt atacatattt gggaatactt ttacaaccct
     6661 ttaagagata cttttccacg tttctccccc tatgacaata tggtctagta atttaatacc
     6721 aacaagatct aatgcttccg agagcctttt cgtaaagagt atatcttctt cacttggact
     6781 aggatctcca gagggatggt tatgtgctat taccactaaa gctgcattta attctagtgc
     6841 tggcgttatg atatctcttg gcaatacgct aacgatatcc aacgacccca taccaactac
     6901 cctcttgtcc agtacctcaa acctagaatt aagaaaaagc ccaactacat actcctgctt
     6961 tttactacca atatctctta acaaaccata cgcatcttca gaatccctca ctaaactcct
     7021 ctcgctctta cacagaccat atactcttcg ccccaactcc atacctgcga gaatacgcat
     7081 tgccgttaca attccaactc cctctactcc atcaatatct tcaatacaaa tatccttacc
     7141 attgctcaca accttccgta ttctcttaac aatactcctt gaaatggaga aaaagtcttt
     7201 tccttttaca ccagaaccaa ccaatattgc taccaattct gtatcactaa gactatctat
     7261 ccctctctca aggtatttct ctctcggaag catgtatata tcatagaaga catatatgaa
     7321 attactctga attcttcttt aaattcttgt cctgtttact ttttctcttt tagaatgttg
     7381 ttctgtatgt ttttggtaat accctcgaac ttttttcttg taattatctc cattcatttt
     7441 gaaatattga aaaatttcta agtaatacct tcgaggtata tcggatggcg tattatagcc
     7501 cttttcaaga agatatttct taatatattt catctgtttt tctgtaaata tcttctttaa
     7561 agtatcaata acgaatctat ttgtctggtt aaatatgtac gaaacaaaat ccctgtacag
     7621 ttcaaattca acctcaggat tttccctctt tacaatctta agtaaaacaa tatctacgct
     7681 tgggactgga ctgaaatcat atctatcaag ttcttctacg attgagaatg tgtatcttga
     7741 gcttagaata gtggcaatct gggtattaaa tggttctcct agaaatctat ctgccgcttc
     7801 ttcttgtacg aaaagaaatg cttctatcaa gctagagtct atggccgtta tcttatttat
     7861 aatctcggat gttaacgcaa atggtatatt actaaatatt ttgaaatctt tcgtaggtaa
     7921 gtttgtaaga agaaaatcat catttatgag tttaatcgga agagaagaaa gattcttggt
     7981 tagctctcta aaatactttg tatctaattc aaaagccgtt acattatctg aatatttgga
     8041 tagttcttgt gctattaccc cttttcctgc accaatatct aatactatgt catctgagga
     8101 gaaagaggca tttttaacca tctccttgag atgattagca tttttataga aattttgtga
     8161 ataacagata tctaccatca acaaattata ccaccttctt tttcttctta gccttgaaac
     8221 tcttcttaat atatttacac tttgggtatg tactacaacc aatgaatgtt ttaccccatt
     8281 tacttcttcg ctctaccaaa ttaccaccac actctggaca cttctcctga agaagcaatg
     8341 gaagagttcc tttacactca ggatacttct cacaagccca gaacttacca tatttactat
     8401 tcttgagtag caatttact
//
LOCUS       DAOQWP010000024         7826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_9735_length_7826_cov_8.289023, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000024 DAOQWP010000000
VERSION     DAOQWP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9735_length_7826_cov_8.289023"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..136)
                     /locus_tag="PKI16_03160"
     tRNA            complement(61..136)
                     /locus_tag="PKI16_03160"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(101..103),aa:Phe,seq:gaa)
     gene            complement(171..1265)
                     /locus_tag="PKI16_03165"
     CDS             complement(171..1265)
                     /locus_tag="PKI16_03165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNR53448.1"
                     /translation="MKNRVAFVTESLFSMGGANRVLEKFLDIYPQADIYALFGSKEDI
                     SKKINKSNIHFSFLNKLPFIKKIYRYTYPLWPFAIEQFDFSNYDLVISFSSSVAHGVI
                     TQVYCKHICYLHSPMRYAWDMRDIYTKRVGEKYGIFKFIWIMLLNKMRVWDTSACNRP
                     DIIITNSNFTRDRAQKYWGRKIDHVIYPPVDEYVGKIYENRESYFVSGAPFEPNKGGD
                     FLLECASKLGFELKIVGTGSMKGKLKRKYQRYKNIHFLGKISDKEKWELLSSASGYIQ
                     CGIEDFGIFPIEAMLCGIPVLAYKGGGIVESLSEGSTGLFFSTLDVAEFEKVFKRFER
                     EKWNHSKIAKYAKRFVKNDFKEKVLRIANN"
     gene            complement(1255..>1668)
                     /locus_tag="PKI16_03170"
     CDS             complement(1255..>1668)
                     /locus_tag="PKI16_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53449.1"
                     /translation="STIDNENGVVMLTGFTQSGSGTLYKTGAKPDVMARLAFKIVKPG
                     NTVLDWEFDNGDNLFQSVMLKDGSPPTNILRVKPDSAIFKIGAKQYTPINTAIPFDKA
                     ILVSGVILIAFGGLMVFAKPRNFTKGKGTIIVYEK"
     assembly_gap    1669..1768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1807..2595
                     /locus_tag="PKI16_03175"
     CDS             1807..2595
                     /locus_tag="PKI16_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNR53450.1"
                     /translation="MENLKIFFLAVVQGISELLPISSSGHLILFGRLIEMEVSTLLLT
                     TLHIGTSLAIIVFFRKRLFSNLFTKKKLVFYLKILMSIIPAAILGVLYEDKVEQILRY
                     TWIIAASLIFWGILMIIADGIKIPRRYKNIEDIPWKKALIIGISQIIALVPGTSRSGI
                     ATLTGITLGIDKYRAFEYSLILGIPILLGSSLWEIWKALTIEPTYPVELLPAVLLKIG
                     IVIIVPFVVGYASLVILKKVKREKWLTVFGIYRIILGTVILLFL"
     gene            complement(2590..4239)
                     /gene="gpmI"
                     /locus_tag="PKI16_03180"
     CDS             complement(2590..4239)
                     /gene="gpmI"
                     /locus_tag="PKI16_03180"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNR53451.1"
                     /translation="MNIFKKQFKSGEDPQRVLDVRKFVTLIIMDGFGIHPDPEGNAVL
                     AAKTPFLDTVWTYGRSTLIHASGTHVGLPSEEAGNSEVGHLNIGAGQVVYQTLPRIND
                     AISAKELDTNPVIKEMIDLVHKRGTKLHLTGVLSAAGVHGHIKHLFSLLEVCKAYGID
                     PYIHIMLDGRDTGQKDGYLYVSKLVEKIDELGVGKIASIMGRFYGMDRDSRWERTKLA
                     YECMVGIADETFVDPREIVQRAYTLGEDDEHFKPRVCVDAQGLPVGVVKEGDAVLFWN
                     FREDRSRQLTKVFVQKDFTHFSRRNYPNDIYFATMTGYEEGLTANVVFPPLPVKKSLA
                     EYLSENGKKQIHISETEKYMHVTYFFNGGIEAPHEGEDFFNIPSPKVDNYAQVPEMSA
                     LLIRDEAVRRIKDIATNDYSFILMNFANPDMVGHTGDFNAAVRSNEIVDECVRDVTKE
                     TLAMGGAVVITADHGNCETMINRVTKEIDIAHTNNPVPLILLNDMEEVYPKAGIPIRK
                     IGTGPNARQTGLLADIAPTCLGLIGMETPANMTGVDLRAVL"
     gene            4284..5369
                     /gene="prfB"
                     /locus_tag="PKI16_03185"
     CDS             4284..5369
                     /gene="prfB"
                     /locus_tag="PKI16_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNR53452.1"
                     /translation="MVDLRKKDIQKLSTDLIDIVKNLDLEKKRERLLNLQNETLKENF
                     WEDSVHAKEVMREIGALQEEINSIDSLKGDINSLVEIFDSTTKETEQNDLIKEYESLR
                     ERLDKFSMMKFLSGKYDSADCILSIHAGQGGTEADDWTQILFRMYTMYCDKQGWKYEV
                     LNMVKGNEAGISTVTISISGKYAYGMLKKESGVHRLIRISPFNAQGLRQTTFAGVEVL
                     PMMEELDKEIDIPEKDLEFKAVLSGGPGGQSVNKTSSAVMITHIPTGITIHSSNERSQ
                     AQNRQNAMKILKAKLWKIKQEERVNEISQIKGDYKIAGWGNQIRNYILYPYKLVRDLR
                     TNVESSDPEYVLDGNLDKFIEAQTKLK"
     gene            5412..6086
                     /locus_tag="PKI16_03190"
                     /pseudo
     CDS             5412..6086
                     /locus_tag="PKI16_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            6331..7257
                     /locus_tag="PKI16_03195"
     CDS             6331..7257
                     /locus_tag="PKI16_03195"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNR53453.1"
                     /translation="MVKTSKIIDSAKKNVKRNKWLSLSTIFVATIVLTISSFFISAAI
                     IGRKAVDYYEQKAQVIIFFKKETAESDILTLRDKLFDPNIVEDIQYVSQEEALKIYQE
                     DFADNPDLISTVTADSLPSSLEIRAKSVEDLLTVIETINVEKQTNPNIDEVMYFKDVV
                     DNLRTLSNIINIGAVLLISALLIITFSLIRITIGFNINAHKEEIQIMNLVGSTDDFIK
                     TPFVIEGTFYGMIGGLLAATLIIVPWYIVVMYTQGTDFSYWLNQTLIDFNLEFLKSVN
                     ITFVPIYYVIHLVFGGLIGMISSISAVKKYLQ"
     gene            7257..>7826
                     /locus_tag="PKI16_03200"
     CDS             7257..>7826
                     /locus_tag="PKI16_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53454.1"
                     /translation="MNKKRIIKILGILLFVTLSFVLLSKPIIPVSEVYAVSCPAGINP
                     DSIECLDYLRDQEEKLIKQQGSIQRQLGNEEYQQLSLQEKITYMTNQIEQTEKVIKSL
                     EVEIAANDVEIKLLEKDILEKEDYISILRQETNILEQTVNQRITESYKYSFVGPLEIF
                     LDVKSLSSALRRTKYLIATRSQDIVSLEEY"
BASE COUNT         2599 a         1418 c         1292 g         2417 t
ORIGIN      
        1 tcgaacccgc gacctacaca ttggcaatgt gtcgttctag ccaactgaac tactctcgca
       61 tggtgccgag acccaggttc gaactgggga ccctttgttc ttcagacaaa tgctctacca
      121 gctgagctat ctcggcattt acaagataat aacaagtaaa gaggaaattt tcaattattt
      181 gctatcctta acaccttttc tttaaaatca tttttaacaa accttttagc atattttgca
      241 atcttactat ggttccactt ctctctttca aatcttttaa acactttctc aaattctgct
      301 acatccaaag tactaaaaaa caaaccagta gaaccttcac ttaaactctc cacaataccc
      361 ccacccttgt aagcaagaac aggaatacca cacagcatag cttcaattgg gaaaatacca
      421 aaatcctcaa ttccacactg aatatatcca gaggcacttg aaagtaattc ccacttctct
      481 ttatcggata tctttcctaa aaaatgtata ttcttgtatc tttggtattt cctttttaac
      541 tttcccttca tacttccagt acctacaatt ttaagctcaa aacctaattt acttgcacac
      601 tctagaagaa aatctccacc cttatttggc tcgaaaggag caccagaaac aaaatagctc
      661 tccctgtttt cgtatatctt tccaacatat tcatctacag gaggatatat tacatggtct
      721 atttttcttc cccagtattt ctgagcacga tctcttgtaa agttggagtt tgtaattatt
      781 atgtctggtc tattacatgc agatgtatcc cataccctca ttttgtttaa gagcataatc
      841 caaatgaatt tgaatattcc atatttctcc ccaacccttt tggtatatat atccctcata
      901 tcccaggcat atctcatagg ggagtgaaga tagcagatat gcttgcagta aacctgagta
      961 attactccgt gagctacact tgaactaaag gagattacta aatcatagtt ggaaaagtca
     1021 aattgttcta tggcaaatgg ccacaaagga tatgtatatc tgtaaatctt tttaataaag
     1081 ggaagtttgt ttaaaaagga aaaatgtata ttggatttat ttatcttttt tgaaatatct
     1141 tccttacttc caaacaaagc atatatatct gcttgagggt atatatctag gaatttttcc
     1201 aacaccctat tcgcaccacc catgctgaaa agcgactctg tgacaaaagc tactctattt
     1261 ttcatagaca atgatagtac ccttaccctt cgtgaaattt ctaggtttgg caaataccat
     1321 taaaccacca aaggcaatta agattacacc acttactaaa attgctttgt caaagggaat
     1381 ggcagtatta atcggagtgt actgttttgc tccaatctta aatatcgctg aatctggctt
     1441 gactctgaga atatttgttg gagggctacc atcttttagc atcacactct ggaaaagatt
     1501 atccccattg tcaaattccc aatccaatac tgtattacct ggctttacaa tcttaaatgc
     1561 taaccttgcc ataacatctg gtttagctcc tgttttatac aaagtgccac taccagattg
     1621 tgtgaaccca gtaagcatga ctacaccatt ttcgttatct atggtactnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnngt tgtcatagtt aaaatattac cataatatat
     1801 ggtggtatgg aaaatctaaa aatatttttc ttggcagtag tacaaggaat atctgaatta
     1861 cttccgatat ctagtagtgg gcatcttata ctctttggga gattgataga aatggaagta
     1921 tctacactcc tacttactac actacacata ggaacaagcc ttgcaattat tgtctttttt
     1981 agaaaaaggc ttttctcaaa tctttttacc aaaaagaaac tcgttttcta ccttaaaatt
     2041 ctaatgtcta ttatcccagc agccattttg ggagtacttt atgaggacaa ggtcgaacag
     2101 atactaagat atacatggat tattgctgct tcattgatat tctggggaat acttatgata
     2161 attgcagatg gtataaaaat accaagaaga tacaaaaaca tagaagatat tccatggaaa
     2221 aaagcattaa ttattggaat atctcagata atcgcgttgg taccagggac atctagatct
     2281 gggattgcaa ctttaacagg tataactttg ggaatagata agtacagagc tttcgaatac
     2341 agtttaattt taggaatccc tatccttctt ggctcatctc tctgggagat atggaaagcc
     2401 cttactattg aaccaaccta ccccgttgaa ttacttcctg ctgttttgct taagattggc
     2461 atagtcatta ttgttccttt tgttgttgga tatgcctctc ttgtaatact taagaaagta
     2521 aagagagaaa aatggttaac cgtatttgga atttatagaa taatattggg aacagtcata
     2581 ttactctttc tataatacag ctctcaaatc tacacccgtc atattcgcag gtgtctccat
     2641 gccaattagt cccagacatg taggggcaat gtctgctagc aagccagttt gtcttgcatt
     2701 tggcccagtc cctatctttc taattgggat acctgcttta ggataaacct cttccatatc
     2761 attgagtaga attaatggaa ctgggttgtt tgtatgagca atatctatct ctttggtaac
     2821 cctgtttatc atggtctcac aatttccatg atctgcagta attactactg cccctcccat
     2881 tgccaaggtt tctttagtaa catctcgaac acattcatct acaatttcat tgcttctaac
     2941 tgctgcatta aaatctccgg tatgccctac catatctgga tttgcgaaat tcatgaggat
     3001 aaaactgtaa tcatttgttg ctatatcctt aattcttctt actgcctcat ctctaattaa
     3061 cagtgcactc atttctggta cttgagcata attatctacc ttaggagaag ggatattaaa
     3121 gaagtcttct ccctcatgtg gagcttctat accaccatta aagaagtatg tcacatgcat
     3181 gtatttctca gtctcagata tatgtatctg cttcttacca ttttctgaca aatattctgc
     3241 aagtgatttc ttaactggca ggggtggaaa tactacatta gcagtcaatc cttcctcata
     3301 acctgtcatt gttgcgaaat agatatcatt gggatagttt cttctactga agtgtgtaaa
     3361 atccttctga acaaaaacct ttgttagttg tctagatctg tcctctctaa agttccaaaa
     3421 aagtacagca tctccctcct ttactactcc aactggaagc ccctgtgcat ctacacacac
     3481 tcttggttta aaatgctcgt catcttcacc tagggtatat gccctctgca caatctctct
     3541 tggatctaca aatgtttcgt cagctatacc aaccatgcac tcatatgcca atttggtcct
     3601 ttcccaccta ctatctctgt ccatgccata aaatcttccc attatactcg caattttccc
     3661 aactcctagt tcatctatct tctccactag tttactaaca tacagatatc catctttctg
     3721 tcctgtatct cgaccatcga gcatgatatg aatatatgga tcaataccat aggctttaca
     3781 tacctccaag agagagaaaa gatgcttaat gtgtccatga acaccagcag cactaagaac
     3841 tcctgttaga tgtaatttag ttcctctctt gtgaacaaga tctatcattt ccttaattac
     3901 gggattggta tccagctctt ttgcagatat tgcatcatta attcttggta gtgtttggta
     3961 caccacttgc cccgctccaa tattcaaatg tccaacctca gaatttccag cttcttcaga
     4021 tggaagccct acatgtgttc cagaagcatg tatcaatgta gaccttccat aggtccatac
     4081 agtatctaga aatggggttt ttgcagctag tacagcattc ccctctggat ctggatgtat
     4141 accaaatcca tccatgataa ttagagttac aaactttcta acatctaaaa ctctttgagg
     4201 atcctctccg ctcttaaatt gttttttaaa tatgttcatt tggcagtagt aataatttga
     4261 gaccttaact ataatatacc ttaatggttg atttacgcaa aaaagatata caaaaattaa
     4321 gtactgatct catagatata gtaaaaaatc tagatctaga aaagaaaaga gaaagacttc
     4381 tcaatcttca gaacgaaaca ttgaaagaaa atttctggga agattctgta cacgcaaagg
     4441 aagttatgag agaaattgga gccttgcaag aagaaattaa cagtattgac tctcttaaag
     4501 gagatattaa ttctcttgta gaaatttttg actctactac aaaggaaaca gaacagaatg
     4561 atctcattaa agagtacgaa tctctcagag aaagactaga taaattttct atgatgaaat
     4621 ttctctctgg aaaatatgac agcgcagatt gcatactttc aatacatgca ggacagggag
     4681 gaactgaagc tgacgattgg acccaaattc tttttaggat gtataccatg tactgtgaca
     4741 aacaaggatg gaaatacgag gtattaaaca tggtgaaagg aaatgaagca gggatatcta
     4801 cagttacaat ctcaatatct ggtaaatatg catatggaat gctcaaaaaa gaatctggag
     4861 tacacagact cattagaata tctccattta atgcacaagg tcttagacaa actacctttg
     4921 ctggtgtaga ggtattgcca atgatggaag aattagacaa agagattgat ataccggaaa
     4981 aagatttaga atttaaggcc gttctctcag gagggccagg gggacagagt gtaaataaga
     5041 cttcatctgc agtcatgatt acacatattc caactggtat tacaatccac tcttccaatg
     5101 aaagaagcca agcacaaaac agacagaatg caatgaaaat actcaaagct aagttatgga
     5161 agattaaaca ggaagaaagg gtaaatgaaa tatctcaaat aaaaggagat tacaaaatag
     5221 ctggatgggg aaatcagatt agaaattaca tattataccc atataaacta gtaagagatt
     5281 tgcgtacaaa tgtggagtcc tcggatccag agtatgtctt agatggtaat cttgataaat
     5341 ttattgaagc gcaaaccaaa ttaaagtaag atatatggta tcctatacaa ttaaactgtc
     5401 taattctgct catgttactt tttgataaag ttacaaaaaa atacaacgaa catatagttg
     5461 ctcttgatga tgtaaatttc aaggtagata ctggagaatt tcttttcatg gtaggtccat
     5521 ctggtgctgg taaaacaaca cttatgagac tgctcattcg cgaagagtta cctacatcag
     5581 gcagtatctt ctttgacaaa atcgatgttc caaagcttcc ttctcgcctg ctcccaaatt
     5641 acagacagag attgggagta gtttttcaag atattaaact tttaagtaac aagacacttc
     5701 tagaaaatat agagtttgcc ttggagattc ttgggaaaga gaacaaagag attaaagaaa
     5761 cttctgaata tctcttagag actgttggac ttcaggatag aagaaacctg ttcccagctc
     5821 aactctctgg tggagagcaa caaagaggag cgatagctag ggcattagca aacaatccgg
     5881 agttattcgt tgcagatgaa ccaacaggaa acctagatcc agacaatgct ttccaagttt
     5941 tagatatact gcgtaagatt aacaaatctg gcacaacagt aatggtaatc acacatgaca
     6001 gagatattgt gaacgatatg aagactagag tcattagaat agacaaaggc aaaataattg
     6061 gagatagtca gggcgattat gatcatcata aagctccgaa gaaagaaact gttgaaatta
     6121 agaaagagga aagtgtagaa attaaagatg agaatctaaa agaattagat aaagatattc
     6181 tagagatatt gtctaaagca aaaattgata acatagaaat ggttctcaat cttacagatt
     6241 cagatttaga taatttgaaa ttctctaaag aacaaaaaga ttctctagag aaataccttg
     6301 aaaaatattt aaataaaaag gagaagtaaa atggtaaaga caagtaaaat aatagattct
     6361 gcaaaaaaga atgttaagag aaataaatgg ctttctcttt ctacaatatt tgtagctacc
     6421 atagtcttaa caatatcttc cttcttcata agtgcggcaa ttattggaag aaaggcagta
     6481 gattactacg aacaaaaagc tcaagtaatc attttcttca agaaagaaac agctgagtca
     6541 gatattctta ctttgaggga taaacttttt gatcctaata ttgtagaaga tattcaatat
     6601 gtatcccaag aggaggcatt aaaaatatac caagaagatt ttgcagataa tccagatttg
     6661 atatctactg ttactgcaga ttcactacca tctagtttag agattagggc taaatctgtt
     6721 gaagatcttc ttacggttat agaaacaatt aatgttgaga aacaaactaa tcctaatata
     6781 gatgaggtta tgtattttaa agatgtcgtt gataatttga gaactctttc aaatataata
     6841 aacataggtg ctgttctgtt aatttctgct ctcttaatca tcactttctc cttaattaga
     6901 attacaattg gttttaatat taatgcacat aaggaagaga ttcagattat gaatttagtt
     6961 ggtagtacag atgatttcat aaagactccc ttcgtaattg agggtacttt ttacggtatg
     7021 attgggggtt tacttgcagc tacattaata atcgttcctt ggtatattgt agttatgtat
     7081 acacaaggta cagatttttc gtattggcta aaccaaactt tgatagattt taatttggag
     7141 tttcttaaat ctgtaaatat tactttcgta ccaatatact atgtaataca cttagtattt
     7201 ggtggtctaa ttggtatgat tagtagtata agtgcagtta agaagtattt acagtaatga
     7261 ataaaaaaag aattataaaa atacttggaa tactactttt tgtgacactt tcttttgtgt
     7321 tattatctaa accaattata cctgtatctg aggtttatgc tgtatcttgt cctgctggga
     7381 taaaccccga ctctatcgaa tgccttgatt atctaagaga tcaagaggag aaactgataa
     7441 aacaacaggg aagtatacag agacagttgg gaaacgaaga atatcaacag ctctctcttc
     7501 aagaaaagat aacatatatg accaatcaaa tagaacaaac agaaaaggtc ataaaatccc
     7561 tagaggttga aatagctgca aatgatgttg agataaaact ccttgaaaaa gatatccttg
     7621 aaaaagaaga ctatatctct atcttaagac aagaaactaa catattagaa caaacagtaa
     7681 accaaaggat tacagaatca tacaaatatt cgttcgttgg accattagaa atattcttag
     7741 atgtaaaatc tctttcatca gcacttagaa gaacaaaata tttaattgcc acaagaagtc
     7801 aggatattgt ttcattagag gaatat
//
LOCUS       DAOQWP010000025         7428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_10351_length_7428_cov_5.050183, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000025 DAOQWP010000000
VERSION     DAOQWP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10351_length_7428_cov_5.050183"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PKI16_03205"
     CDS             complement(<1..223)
                     /locus_tag="PKI16_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR53455.1"
                     /translation="MLSTLKRRYPAYTLLELLLVLSIFSIIGGMTFASFDGLQNTVKM
                     NEYMLNLEQDVRTIQRESMLLERTPGEDWL"
     gene            complement(281..862)
                     /locus_tag="PKI16_03210"
     CDS             complement(281..862)
                     /locus_tag="PKI16_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR53456.1"
                     /translation="MKQRYKAFTLIELIVVIGILIILMAIGFAIGRYAIQKSQDTYHQ
                     SAVRNLYAALVKYKIDNKEYPEVGACSGCIEEEFFSYALGYNGTSDQHILVSYLEEEG
                     KFDGGTDATYYYAVDEYDQQFVIVCVSLGGVDDENQKGFYCTGDGIGILPGDSPILSA
                     DIGSLESGDPSALSVLSLDDSDWRRKDQFTQSQ"
     gene            complement(874..1623)
                     /locus_tag="PKI16_03215"
     CDS             complement(874..1623)
                     /locus_tag="PKI16_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNR53457.1"
                     /translation="MIIFYLCIFFLGASLASFLNATVYRLEKGYKYPKIVTLNSHCEK
                     CNKPLNWKQLIPILGYIFYKGKCPKCKSVVNIYYPISELILGTIFLLFYLSSIPLFFY
                     IIILFLFVLSFYDYLSNSIPKNLVHILIGICLVFFFFFNFNLENIYLPLIVCGALLII
                     NIFKKSFGFGDILLLFAIGILQSFGEFMVTFWFAIFIALLYSLIFVVKKKVDIKKAKI
                     PMAPFISLAFSISILYGENIYSQLLKWMGIW"
     gene            complement(1691..2239)
                     /locus_tag="PKI16_03220"
     CDS             complement(1691..2239)
                     /locus_tag="PKI16_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNR53458.1"
                     /translation="MRRNFYEAFTLVEMLIVMGILIILMAIGITAGRFAINRANDVAH
                     QNAVDQIYQGLQAYYTDERAFPDQQLPSALITTLDEYMDEGVFKGGTEATFMYIVEPT
                     HLQSLLVCVSLRGLATDNNTRDDGAYYCNGNGFGDDEMPITITNKNFENGTAPDADFT
                     ALVAYYTANSATIAADWDGNAW"
     gene            complement(2305..3516)
                     /locus_tag="PKI16_03225"
     CDS             complement(2305..3516)
                     /locus_tag="PKI16_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR53459.1"
                     /translation="MKKFKYSARDTSGKIVNGDMEAKDAGAVTDILHDRGLIIVTIKE
                     GFGLDLERLSEINIGGVPMKEKVIFMRQMSTMVGAGLPLTRALEIMVQQTSNPMFKRV
                     LKEVLAAIQSGKTLANSFREQSEVFDPITLNLIEAGEESGNLETILERLATELEESKT
                     LGDKLRSAMIYPIIILVIIVVVMVLMMLVLVPSMASIYSDFGAELPFATRLLMDMSTF
                     FTKFWWAILVVLAVVFIGMKYYLDTAKGKRNFDKIVLKIPAIGTIISKMQLSQFTRIL
                     ALLLGSGLSIIKALELTAQSLKNEMFRDTVMAAKEEVEKGGALAIPIARSEYFPLLVS
                     SMIAVGEETGEIDAVLVKVSQYYKEEVDVATSNLSSILEPLFLVIMGLAIGFIALAVY
                     MPMFQLSSAIG"
     gene            complement(3520..4980)
                     /locus_tag="PKI16_03230"
     CDS             complement(3520..4980)
                     /locus_tag="PKI16_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNR53460.1"
                     /translation="MENQTAQNSNTKFPYPDFANTNQPAQTTTTPPTLPEPDVTEIKY
                     VAKPTEMPGLKTESSDVGILPSEDSAPVSSAVSTLNSIGKAPDSLNDIMPDFKKPKAM
                     VREPKANNLAEPILKQEEIKKAEELIQPANGKYTIEELFSITINKNASDLHLSPGYPP
                     IIRIDGELEEVGEKILTPQDTEELIFGTITDEKKELLEVNREIDYAYMYAEGNNSRFR
                     VNAYYSMKNLSAAFRLIPSRIRTIEELLLPQLYHQFAKLKQGLVLVTGPTGHGKSTTL
                     AAILEDINRTRFCHIVTIEDPVEYLFEGKRALIDQREMNDDTHSWEIALRSALRQDPD
                     VILVGEMRDFETIAAAITLAETGHLVFATLHTNSSSQTLDRIIDVFPETQQQQVRAQL
                     SNILQAVVAQRLIPLDKGGRRAVSEIMIMNSAVGNLIREGKTHQIDNVIRTSSDLGMV
                     SLEKALVNLVREGAINIQKAQEYAVFPEEVLRLLKA"
     gene            complement(5003..6808)
                     /locus_tag="PKI16_03235"
     CDS             complement(5003..6808)
                     /locus_tag="PKI16_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNR53461.1"
                     /translation="MDSKSSLLKYFETKGLIKEEDARRVLVERSRSPKTEEVIIKELG
                     LVSEDDLAKAKSDIFGIPYIDLRDMEIPEGTIAEVNVDGLQKYRAVPFERGKDFVKIA
                     MEDPFDVQATQALERKYPQGTRLIVNITTRESINSVLDRRVGEVMSSEVTEALEDVDV
                     PVTEISEDPSLLSSNNLSNAPVARIANAILQYAAISKASDIHIEPMEKRLRVRFRING
                     VMTERLTLPRDLGPALISRVKILANLKIDEKRLPQDGRFPIKVNGSKIDLRVSIMPTI
                     FGEKGVLRLLESETDGITLESTGLRGNAYKIFLDSLDVTNGIVLVTGPTGSGKTRTLA
                     CSLMKVNDPKVNIISLENPVEIRVPGVTQVQINPDAGLTFASGLRSVLRQDPDIVMVG
                     EIRDQETAELAVQASLTGHLVLSTLHTNSAAAAIPRLLDMGIEPFLLSSTIRAVVAQR
                     LPRKVCQNCIEAYPAPPEIVENIKEIMSTVKGFNLVEYLKRVGASKASSVEGDKNFFR
                     VPEIGPGGEQIIYLYRGKGCDKCGGSGYSGRIGIFEVLDITDKISRMMMESVSDKDIE
                     KVALENGMLTMTQDGYLKALEGITSLEEVMRVSKE"
     gene            6878..>7428
                     /locus_tag="PKI16_03240"
     CDS             6878..>7428
                     /locus_tag="PKI16_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53462.1"
                     /translation="MIESDMTYLLINNNPKVIEKNIVLLANEFLGENFEKLEDLFAVP
                     DIHIVDGRRSNSIGIEEVKELQNEMKYKPFDNHSQLAIILDSQKLTLQAQNSFLKTLE
                     ESSDNTIYILCVNNEKNLLQTIISRGKLIYAKGEVEKVENDTSNILELDLVEQFLLVE
                     KVAKDKEASLGLIEDLEKVFKKKL"
BASE COUNT         2259 a         1452 c         1231 g         2486 t
ORIGIN      
        1 acaaccaatc ttccccaggg gttctttcaa gtaacataga ttctctttga atagttctta
       61 catcttgttc caagttaagc atgtactcat tcatctttac tgtattttgt aggccatcaa
      121 atgatgcaaa ggtcattcct ccaattatgg aaaatataga gagcacaaga agaagctcta
      181 aaagagtata tgcgggatat cttcttttga gagtagaaag caaggcagta tatacttaat
      241 tcaatactta aatgtatcac agaaagacgc gagtatcaat ctattgactt tgagtaaact
      301 gatcttttct tctccagtca gaatcatcta ggcttaagac tgaaagagca gatggatcac
      361 cactttcaag agaaccaata tctgcgctga gaatagggga gtctcctggc aaaataccta
      421 taccgtcacc agtgcagtaa aaaccttttt ggttttcatc atctacacct ccaagagaaa
      481 cacaaacgat tacaaattgt tggtcgtatt catcaacagc gtagtagtat gtagcgtctg
      541 ttccaccatc aaatttccct tcttcttcca gatatgagac aagtatatgt tggtctgaag
      601 ttccattgta tccaagggcg tatgaaaaaa attcctcctc tatgcatccg ctacaagctc
      661 ccacctctgg gtattcttta ttgtctatct tgtatttgac caaagctgca taaagatttc
      721 ttacagcact ttgatggtat gtatcttggc ttttctgtat agcatacctt ccgatagcaa
      781 aaccaatagc cataagtatg attaaaatac cgattactac aatcaattca attagggtga
      841 aagctttgta tctctgcttc atatgataat aatctaccat attcccatcc atttaagaag
      901 ttgagaatat atgttttctc catataggat agatatacta aacgccaaag aaatgaatgg
      961 agccatagga atctttgctt ttttaatatc tactttcttc ttaactacaa agattagaga
     1021 gtagagtaga gctatgaata ttgcaaacca gaatgtaacc atgaattctc caaatgattg
     1081 cagtattcca atcgcaaaaa gaagaagtat atctccaaat ccaaaagatt ttttgaatat
     1141 atttattatt agtaatgctc cacacacgat caatggtagg tatatatttt ctaaattgaa
     1201 attaaagaag aagaaaaata caagacaaat cccgatgagt atatgtacaa gatttttagg
     1261 gattgaatta cttagatagt cataaaaaga gaggacaaaa agaaaaagaa tgattatata
     1321 aaagaatagg ggaatacttg agagatagaa gagaagaaag attgttccaa gaattaattc
     1381 tgatattgga tagtagatat ttacaacaga tttacattta ggacatttgc ctttgtaaaa
     1441 gatatatcca agaataggga ttagttgttt ccaattaaga ggtttattac atttctcaca
     1501 atgggaatta agagtaacaa tctttggata tttgtatcct ttctctagtc tgtatacagt
     1561 agcgtttagg aaactagcta gggatgctcc tagaaagaat atgcataggt agaaaataat
     1621 catttttgat tatagcatag taaaagaaaa acccctccga agaggggttt aagattccaa
     1681 ttcttaatga ttaccatgca tttccatccc aatcagcagc gattgttgcg gaatttgcgg
     1741 tgtagtaagc aactaatgct gtaaagtctg catcaggtgc tgtaccattc tcgaagttct
     1801 tatttgttat ggttataggc atttcgtcat ctccaaatcc atttccgtta caatagtaag
     1861 caccatcatc tctcgtattg ttgtctgttg ctaaacctct aagacttaca cataccaaca
     1921 aggactgtaa atgtgttggc tctacgatat acataaatgt tgcttctgtt ccacctttga
     1981 aaactccctc atccatatat tcatcaagag ttgtaatcaa tgcagaaggt agttgttgat
     2041 cagggaatgc tctttcatca gtataataag cttgtaatcc ctggtatatc tgatctacgg
     2101 cattctggtg tgctacatca tttgctctat tgatagcaaa tctaccagct gtaataccga
     2161 tagccataag gattataagg atacccataa cgataagcat ctctactagt gtaaatgctt
     2221 catagaaatt ccttctcata tatttggaac agttataaat ttatctatta ataagttaca
     2281 taactttcaa atccatgtca atagctaccc aattgcagag gatagctgga acataggcat
     2341 atacacagcc aaagctatga aacctattgc caatcccatt attactaaga ataatggctc
     2401 taaaatcgaa gataagttac tcgtagcgac atctacctct tctttgtaat actgagaaac
     2461 tttaactaaa acagcatcta tttcaccagt ctcttctcct acagcaatca tactgctcac
     2521 caacagaggg aagtactcac ttcttgcaat tggtattgct aatgctcctc ctttttctac
     2581 ctcttctttt gcagccatta cagtatctct aaacatttca tttttcagtg actgtgctgt
     2641 taattccaaa gccttgataa ttgaaagacc acttccaagc agaagtgcca atattcttgt
     2701 aaattgggaa agctgcattt tagaaatgat tgttccaatt gctgggattt ttaaaactat
     2761 cttgtcaaaa tttctcttac cctttgctgt atctaggtag tacttcatgc caatgaaaac
     2821 aacagcaagc acaacaagaa ttgcccacca gaattttgtg aagaaagtac tcatgtccat
     2881 tagtagtcta gtagcaaatg gaagttctgc accgaaatca ctgtatatag aggccatgga
     2941 cggaacaaga acaagcatca tcagaaccat aactacaaca ataatgacaa ggataataat
     3001 agggtaaatc attgcagacc taagcttgtc tcccaatgtt ttgctttctt ctaattctgt
     3061 tgcaagcctt tctaagattg tttccaaatt tccactctct tctcctgctt caataaggtt
     3121 gagtgtaata gggtcaaaca cttcgctctg ttctctaaaa gagttggcaa gagtttttcc
     3181 agattgaata gcggcaagta cttctttgag aactctttta aacatagggt tgcttgtctg
     3241 ttgcaccata atttcaagag ctctagtaag aggcaatcca gcaccaacca ttgtagacat
     3301 ctgtctcatg aagattactt tctccttcat tggaactcca ccgatattta tctctgacaa
     3361 tctttcgaga tccaaaccaa aaccctcttt aatagttacg ataattaaac ctcgatcatg
     3421 tagaatatct gtaactgcac ctgcatcttt tgcttccata tctccattta caatttttcc
     3481 acttgtatct cttgcagagt atttaaactt tttcatttat tatgctttta gtaatcttaa
     3541 aacttcttct gggaataccg catattcctg tgctttctgt atatttatag ctccctctct
     3601 tactagattg actaatgctt tttctaagga aaccataccc aagtctgagc ttgttcttat
     3661 gacattatct atctggtgag tttttccttc tctaattagg ttaccaacag cagagttcat
     3721 aatcatgatt tctgacacag cccttcttcc acccttatct aaaggtatta atctttgtgc
     3781 tacaaccgct tgcaagatat ttgagagttg tgctctaacc tgttgctgtt gagtctctgg
     3841 gaatacatca ataatacggt caagcgtttg agaagaactg ttagtatgta gagttgcgaa
     3901 taccaagtgt cctgtttcag ccaaggttat agctgcagca attgtttcaa aatctctcat
     3961 ctctcctaca agaataacat ctggatcttg ccttagtgcg cttcttaacg ctatttccca
     4021 agagtgtgta tcatcattca tttctctctg gtcaattaat gctctttttc cttcgaataa
     4081 gtactcaaca ggatcttcga ttgtaacaat atggcagaat ctagttctgt taatatcttc
     4141 aagaattgca gctaaggttg tactttttcc gtgaccagtt ggaccagtaa ctagtacaag
     4201 gccttgtttt aatttagcaa actggtggta aagctgtggg agaagcaatt cttcaattgt
     4261 tcgaatacga gatggaatta atctaaatgc ggcagaaaga tttttcatgg agtaataggc
     4321 atttaccctg aacctagaat tattaccttc agcatacata tatgcataat ctatttccct
     4381 gtttacttct aataattctt ttttctcatc tgttatggtt ccaaatataa gctcttcagt
     4441 atcctgtggt gtcaatattt tctctccaac ctcttctaac tctccatcta tcctaatgat
     4501 tggaggatat cccggagaga gatgaaggtc tgaagcattc ttgttaatcg taatactaaa
     4561 aagttcttca attgtatatt tcccgtttgc tggttgtatt aattcctctg ccttttttat
     4621 ctcttcctgt ttcaatattg gttctgcaag attattagct tttggttctc taaccattgc
     4681 ttttggtttc ttgaaatcgg gcataatatc attaagactg tcaggtgctt ttcctattga
     4741 gtttaatgtc gagactgcag atgataccgg agcagaatct tctgagggaa gaattcctac
     4801 atcagagctt tctgttttta agccgggcat ttcagtcggc tttgctacat atttaatttc
     4861 tgttacatct ggttccggca gagtaggtgg agttgtagtt gtttgtgctg gttgatttgt
     4921 attagcaaaa tccggatacg gaaattttgt attactattt tgggcagttt gattttccat
     4981 actatatatt tatatcaaaa atttactctt ttgaaaccct cataacctct tctaatgaag
     5041 ttataccttc taaggccttt agatagccat cttgtgtcat ggtaagcata ccattctcaa
     5101 gtgctacttt ttcaatatct ttgtctgaga cactttccat catcattcta cttatcttgt
     5161 ctgtaatatc caacacttcg aaaataccaa ttcttcctga gtatccagaa cctccacatt
     5221 tgtcacatcc ttttccacgg taaagataaa tgatctgttc tccacctggg cctatctcag
     5281 gaactctgaa aaaattcttg tctccttcta cagatgatgc tttagaagct ccaactcttt
     5341 tgagatattc aacaagattg aatcctttaa cagtggacat tatttcttta atattctcta
     5401 ctatttcggg tggagcaggg tatgcttcta tacagttttg gcatactttt cttggaagtc
     5461 tttgtgcaac aacagctcta attgtagagg atagtaggaa aggttcaatt cccatatcca
     5521 gaagtcttgg gatagcagcg gctgcactgt ttgtgtgaag agtagagaga actaagtgac
     5581 ccgtaagaga tgcctgtaca gccaactccg cagtttcctg gtctctgatt tctccaacca
     5641 ttactatatc tgggtcttgt ctaagaacag atctcaaacc acttgcaaat gtaagccctg
     5701 catcaggatt aatctgtacc tgtgttacac ctgggactct aatttctact gggttttcca
     5761 aagatataat atttacctta ggatcgttaa ccttcataag tgaacatgct agcgttcttg
     5821 ttttaccact accagtaggg cctgtaacta gtacaatacc gtttgttaca tcaagactat
     5881 ctaagaatat tttgtatgca tttccacgaa gaccggtaga ttcaagtgtt attccatctg
     5941 tttcagattc caacaatctt aaaactcctt tctcaccgaa gatcgttggc atgatagaaa
     6001 cacgaaggtc aatcttcgag ccatttactt taatagggaa tcttccatct tgaggtaatc
     6061 ttttttcatc aatttttaag tttgcaagaa tcttgactct tgaaattaac gctggtccca
     6121 agtctcttgg gagagttaat ctctcagtca tgactccatt tattctaaat cttactctca
     6181 atcttttttc cattggttca atatggatat ctgaggcttt agatattgca gcatactgca
     6241 aaattgcgtt ggcaatcctt gctactggag cattactaag attgttacta ctaagtagac
     6301 ttggatcttc actaatttct gtaacaggga catctacatc ctcaagagct tctgtaacct
     6361 ctgagctcat aacttctccg actcttctat ctagtacaga attaatactt tctcttgttg
     6421 tgatatttac aattaatctc gtaccctgtg gatatttccg ctcaagagcc tgtgtcgcct
     6481 gaacatcaaa aggatcttcc atcgctatct ttacaaaatc tttccctcgc tcaaaaggta
     6541 ccgctctgta tttctgtaga ccatctacat taacttcagc tattgttccc tctggaattt
     6601 ccatatctcg caaatcaatg taggggatac caaaaatatc tgattttgct tttgccaaat
     6661 cgtcttcaga aacaagacca agctctttga taattacttc ttctgtcttt ggggaacggc
     6721 ttctttcaac aaggactctt cttgcgtctt cttccttgat tagacctttg gtctcgaaat
     6781 attttaatag cgaactctta gaatccattt ggcagttttt taaataatac tattcttagt
     6841 ttattctaat cccttactac agtcaatatt tatatatatg atagaatcag atatgacata
     6901 tcttctaatt aataacaatc ctaaagtaat cgaaaaaaat attgttcttc tggcaaatga
     6961 attcttagga gaaaattttg aaaaactgga agatcttttt gcagtgccag atatacatat
     7021 tgtcgatgga agaagaagca acagtatcgg aatagaagag gttaaagagc tgcaaaatga
     7081 aatgaaatac aagcctttcg acaatcactc ccagctcgca attattctag atagccagaa
     7141 attgactctt caagcacaga attcttttct taaaacactt gaagagagta gtgacaacac
     7201 tatatatatc ctttgtgtaa acaatgaaaa aaatcttctt caaaccataa tctccagagg
     7261 caaactaata tatgcaaaag gtgaagtgga aaaagttgaa aatgatactt caaatatact
     7321 tgaactagat cttgttgagc agtttttact tgttgagaaa gttgctaaag ataaggaagc
     7381 atctcttgga ttaattgaag atttagaaaa agtatttaaa aagaaact
//
LOCUS       DAOQWP010000026         6826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_11525_length_6826_cov_8.880224, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000026 DAOQWP010000000
VERSION     DAOQWP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11525_length_6826_cov_8.880224"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1708
                     /locus_tag="PKI16_03245"
     CDS             101..1708
                     /locus_tag="PKI16_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53463.1"
                     /translation="MKVPRIKITGNHILSACFVVAAIVGIIYSISLINRTYIQNTLRQ
                     PLWVQFDEEDTEPKFALTKIVSDLVPGDGYDGDNYFFRNKVTAYGDSTSGECYKTQYK
                     CGDGKWHNFEGDSVILENRCLIKIKDSTYCNVILPGGTLIAASVHQKRTDSFDTSDLT
                     FQIISTDEQITIIQSVTNQESILWHSLAPNFNKKYVVEIGDNTAEATGTEFYTSSDET
                     DGVVTTVIKGSVKVYNTTITQGQYAQLPYFNVLACPPALKACGNSCIPANGICCDSNS
                     YCLSPLQCQENPTGTCKANLFSGDSSQYCCYNDPNASSFSLFDKGSYDCEDGYKFCAN
                     KCIPEDQECCYPTDEDCPVTADEVDMGTGDVPSDYEFPRKYFFDLYDRLIADPTYLGL
                     VSATAFTEDTFNFDSVLKYITQYLKPIVEQDYANSNEATEQKIYCDTYHNSGICPSTR
                     TSSSSSTSASGDCSMMNDIKILSCYVESAGTLNKGKPGYVQCQYKIVKNKDKYCTSSG
                     GLVCTGTGEGAIPKKCLPSGFNDWVNP"
     gene            1736..2977
                     /locus_tag="PKI16_03250"
     CDS             1736..2977
                     /locus_tag="PKI16_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53464.1"
                     /translation="MKKLVLPKLHLTKKQIILVSAGLLLLIASVVTYIIFKNPKDGSN
                     TQQTNIQGNTGEGYAESEKAESSSWQKAYSTETDPKILVYGEIADNPWGTEPYSIDLT
                     EECEYEDKETEESTYVLGSTPIEVKNHCILTLSHTVDPTETQDTLTAYLVLPDNSVIS
                     LSSNSFPTIQVNIYEKETRIVQMNSLAYYRISPQEDSHIFTVELPLLSRIFTATGTEL
                     YAEMGFTEGSFPQYDLDEGNYAGGFFLYDGSGNIRKTGSTEILESMDSNDNTNIYYGF
                     LSLTTFQTEAISLVTDTLDMVGRADFLTNQKAWSEKYGFGNFSSFTTDTLKAFVYEHT
                     EDVYAKTVSKVEEMKAEENRKWEEMQDDDDNYSSSSGSSSTGSSSSSESCYAKGSASY
                     QLFCQMSSQGTIKDGKCCVSY"
     gene            complement(3589..4566)
                     /locus_tag="PKI16_03255"
     CDS             complement(3589..4566)
                     /locus_tag="PKI16_03255"
                     /inference="COORDINATES: protein motif:HMM:NF027610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4932 domain-containing protein"
                     /protein_id="HNR53465.1"
                     /translation="MKISVQTNKNVIFFETLISFISNDKDFHPLGQKVIEEFKDIKKI
                     KAFEVFQKNVIEKKIPSHPWQYVFLSINLNDDLTPKSLSPRDGFGPNREKLYTEDLYP
                     IVNRINTESNFGRRYCEKILPEYEIVTQDIQKLFDKENPGDVLEEFWGKELKANLVFI
                     PDPLRVGGGSGVSRGRDFYSITGTVKTGGEIEFKNSHMISNLLHEYSHSFFKNYVYKN
                     EELFKKNEELCNTLSGRIKKDKEIFKSYGTSTVYFEETFIRAVQILLSRRFFEKAMNK
                     DMESKSLELLRKRKEEGFLYIEEFYNKLEDSKDPMASYMHVLEDIMKNL"
     gene            complement(4650..4796)
                     /locus_tag="PKI16_03260"
     CDS             complement(4650..4796)
                     /locus_tag="PKI16_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53466.1"
                     /translation="MAELFGNVVVLAEADTTVANCNPECFGDCQDNCDCYCESYCTDD
                     DDWD"
     gene            complement(4812..6080)
                     /locus_tag="PKI16_03265"
     CDS             complement(4812..6080)
                     /locus_tag="PKI16_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNR53467.1"
                     /translation="MKIISQEILCDDFFLISDDQRNLIFSPLRRIVFEVFDLGIKEIR
                     KCQKDGTIESLLMERAVQEKKAMVNRVERPFKPMRLVLSLTSDCNLRCIYCYAEAGKL
                     HKTMSWETAKSAIDETMRNLLEERKEKFQLTFHGGGEPFVAFKLMKKAVEYVNSKWKG
                     KKDFSVVTNGTLITSEIADWLAEKDFRLTVSIDGPAEIQNRQRPKANGEGSYEDAISG
                     AMLLKERGLKFGIRSTITNANVYHMEDLLKIAKDLDCSLKLEPFTPVGRGVDEEILSQ
                     DAYFKQFMLASKLAKEMGVPLKSTYSSGLDPKCIFCAGDGEMFCVLPDGKVSTCSRVT
                     KGDDFLASTFIVGEINADGLNINPERVEELRHLNVMFYDQCRDCFAKWYCMGGCHNTR
                     LLNDGVMPENHCVLEKAFLWTNLIKAVGSN"
     gene            complement(6382..>6826)
                     /locus_tag="PKI16_03270"
     CDS             complement(6382..>6826)
                     /locus_tag="PKI16_03270"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNR53468.1"
                     /translation="EKDGEGILWGGCLESIDELLRHNVEIPSLEDFKNVIMYLETSEE
                     MPSHNYVARVLRALGERGILGNIQGLLVGRPKAWEFDKQNTDEQKDEYKRGQREIVLE
                     TVRKYNKNIPVVQNMDFGHTAPQICLPSRGNTRVLSSGKKIYCEF"
BASE COUNT         2205 a         1320 c         1123 g         2178 t
ORIGIN      
        1 gtatgtcccg tttttccaat ttctttcaat acttttcttc ccctttgact tcgcaaatta
       61 ctaatgttac attgattaat aactttgcat actctctcct atgaaagttc cgagaataaa
      121 gattaccggt aaccacatac tctctgcttg ctttgtggta gcagcgattg taggcatcat
      181 atattcaata tctctaatta accgaaccta tattcaaaac acactacgac aacccctctg
      241 ggtacaattt gatgaagaag atactgaacc aaaatttgca ttaaccaaaa tagtctctga
      301 tctagtacca ggagatggat atgatggtga taattacttt ttcagaaata aggtaacagc
      361 atatggagac agtacaagtg gagaatgtta caaaacacaa tacaaatgtg gtgatggaaa
      421 atggcataat tttgaaggag attcagtcat cctagaaaac agatgcctaa taaaaattaa
      481 agacagtaca tattgcaatg taatactccc aggagggaca ctcattgcag cttcagttca
      541 ccaaaaaaga accgattcat ttgatactag cgacctcaca ttccaaatca tctcaacaga
      601 tgagcaaatt accataatac aaagtgtaac caatcaagaa tcaatactat ggcatagcct
      661 agcaccaaac tttaataaaa aatatgttgt tgagattgga gataacactg cagaagccac
      721 tgggacagag ttctacacat ctagtgatga gactgatggt gtcgttacta ctgttatcaa
      781 aggaagtgta aaagtttaca ataccacgat tacacaagga caatatgccc aattaccata
      841 tttcaatgtt ttagcttgcc caccagcact aaaggcatgt ggtaatagtt gtattccagc
      901 caacggtata tgttgtgatt caaacagcta ctgcctatca cccctccaat gtcaggagaa
      961 ccctactggt acatgtaagg cgaatctttt ctcaggagac agttcccaat actgttgtta
     1021 caatgatcct aatgcgagta gcttctctct gtttgataaa gggagttatg actgtgaaga
     1081 tgggtataag ttctgtgcaa acaaatgtat tcctgaagat caagaatgtt gctaccctac
     1141 agatgaggat tgtcctgtta cagcagatga agttgatatg gggactggag atgtaccatc
     1201 tgactacgaa ttcccaagaa aatatttctt tgacctatac gatagattaa tagcagatcc
     1261 aacatatctt ggtcttgttt ctgcaactgc ttttactgaa gatactttca attttgatag
     1321 tgttcttaaa tatattacac aatacctcaa gcccattgta gagcaagatt atgcaaatag
     1381 taatgaagcc acagaacaaa aaatatattg tgatacctat cacaattcag gaatatgtcc
     1441 tagtacaaga acatctagta gtagctccac atcagcatcg ggagattgta gtatgatgaa
     1501 cgatattaaa attctttctt gttacgtaga aagtgctggt actttaaata aggggaaacc
     1561 tggatatgtg caatgtcagt acaagattgt taaaaacaag gataaatatt gtacaagctc
     1621 tggagggttg gtgtgtacag gaacaggaga aggagcaatt cctaagaaat gtttacctag
     1681 cggttttaac gattgggtaa atccataaag aagattaatt tttaataatt tggccatgaa
     1741 gaaactcgtt cttccaaaac tacaccttac aaaaaaacaa atcattctag tatctgcagg
     1801 actcttatta ttaatagcat ctgtagttac atatatcata tttaaaaatc caaaagacgg
     1861 aagtaatact caacaaacca atattcaagg aaatacagga gaaggatatg cagaatctga
     1921 gaaagcagaa tctagttcgt ggcaaaaagc atattcaaca gaaacagatc caaaaatact
     1981 tgtatatgga gagatagcag acaatccatg gggaacagaa ccatactcaa tagatttaac
     2041 agaggaatgt gaatatgagg acaaggagac ggaagaaagt acatatgttt taggatccac
     2101 cccaatagaa gtaaagaatc attgtatttt aaccctgtca cacactgtag atccaacaga
     2161 aacacaagat acactaacag catatcttgt attaccagac aactctgtca tatcactaag
     2221 ctcaaatagt tttcccacta tacaggtgaa tatatatgag aaagaaacaa ggattgttca
     2281 gatgaatagc cttgcatatt acagaatatc tcctcaagaa gatagccata tatttacagt
     2341 tgaacttcca cttctttccc gtatatttac agctactgga acagaactct atgcggaaat
     2401 gggatttaca gagggatcat tccctcagta tgatcttgat gagggcaact atgctggtgg
     2461 attcttcctc tatgatggtt ctgggaatat tagaaaaact ggaagtacag agatactaga
     2521 atcaatggat agcaatgata atacaaacat atactatggt ttcctttctc ttacaacttt
     2581 tcaaacagaa gctatttctt tggtaactga tactttggat atggtgggaa gagcagattt
     2641 tcttactaac caaaaagctt ggtctgaaaa atatggattt ggtaatttca gtagttttac
     2701 tacagataca ttgaaagcct ttgtatatga acatacagaa gatgtgtatg caaagacagt
     2761 ttcaaaggtt gaagaaatga aagcagaaga gaataggaaa tgggaagaga tgcaagatga
     2821 tgatgataac tattcctcct caagtggttc atcttctaca ggtagtagta gcagctcaga
     2881 gagttgttat gctaaaggtt ctgctagcta tcagctgttc tgtcagatgt ctagtcaggg
     2941 aactataaag gatgggaagt gttgtgtaag ctattagata ttggcacggg tttgcataga
     3001 taaagggatt tgttaagatg atagcaatag tggtgtaccc actgctggaa tgcagagtgg
     3061 caccactttt tgttttataa tttttcccct tcttaagctt ctaaatcatt tgatgagaat
     3121 gaggtttgca agcagaatat caacctttta gaccagatca tttctatgag aaatactttt
     3181 gcgaaatttg gcttatgtgg tggaatggtg gagactctta tgggatccta tggctagaat
     3241 aagacatttt aatcttcttt tttaggtgtc tcttttgtct catttttggt atcatctagt
     3301 tgggaagata gggtccagac ccgtatgtcc cgccattctt tcaaggttgc ttcaagacct
     3361 gtgcactacc cattagaaag gttgttaaag cttgattatt tcgaggtcga aaaactaagt
     3421 tttcagaaat atggcctagt gtataaaaaa ctttgaaaaa gctccattta ataacccctt
     3481 ccgttcgaag taagaacccc cttgtaacac aacaatttcc atatacccaa atttcacgct
     3541 ccgttccatt ctcgtttctg ttaactatat tttgtggata aaccaatatt ataaattctt
     3601 cataatatct tccaaaacat gcatatagga agccatggga tctttactat cctctaattt
     3661 gttatagaac tcctctatat aaagaaaacc ttcctctttt ctctttctta agagttctaa
     3721 agacttgctt tccatatcct tgttcattgc cttctcaaag aatcttctac ttaagagaat
     3781 ctgtactgct cgaataaatg tctcttcaaa atacactgtg gaagttccat aactcttaaa
     3841 tatctcttta tctttcttta ttcttcctga cagagtattg caaagttctt catttttctt
     3901 aaatagctct tcatttttgt aaacataatt cttgaaaaat gaatgagagt actcatgaag
     3961 aagattagat atcatatgag aatttttaaa ttctatctcc cctccagttt tcactgttcc
     4021 agtaatagaa tagaaatctc ttcctcttga tactccactt cctcctccaa cacgtagagg
     4081 atcggggata aacactaagt tagcttttaa ctctttaccc caaaattctt ctaaaacatc
     4141 tccaggattt tctttatcaa aaagcttttg aatatcctgt gttacaattt catattctgg
     4201 aagtattttt tcacaatatc ttcttccaaa attactctca gtatttattc tatttacaat
     4261 cggataaaga tcctctgtgt ataatttctc tctattaggg ccaaagccat cccttggtga
     4321 taatgatttg ggagtaagat cgtcatttaa attaatagag aggaaaacat attgccaggg
     4381 atgagaaggt attttctttt ctattacatt tttttgaaat acttcaaaag ctttaatctt
     4441 ctttatatct ttaaactctt ctattacttt ctgccctaat gggtgaaaat ctttatcatt
     4501 gctaataaaa gatattaaag tttcaaagaa aattacattt ttatttgtct gtacagatat
     4561 tttcattatt tttaaaagaa ttgcctctgg gagtaaagtg ttttttaccc ccagagggtt
     4621 agttggatac aattaggata gcataaatgc taatcccagt catcatcgtc tgtgcagtaa
     4681 ctttcacagt agcagtcaca attgtcttga caatcgccaa aacattcggg attgcagttt
     4741 gcaactgtag tgtcagcttc tgctagaact acaacattac caaacagctc agccatgatt
     4801 ctcacctcct ttcaattaga gccaacagct ttaatcaagt tggtccagag aaatgctttt
     4861 tctaaaacac aatggttctc tggcatcaca ccatcattca atagtcgtgt attatgacat
     4921 cctcccatgc aataccattt tgcaaaacaa tctctgcatt ggtcatagaa catgacattt
     4981 agatgcctta gttcttcaac tctctctgga tttatgttta atccatcagc attgatctcc
     5041 ccaacaataa atgtactggc aagaaaatca tcccctttag ttactcttga acaggtcgaa
     5101 accttaccat cagggagaac acaaaacatt tctccatcac ctgcacaaaa gatacacttc
     5161 ggatcaagcc cagaagaata ggttgatttc aatgggactc ccatctcttt tgctaacttc
     5221 gaagctaaca taaactgctt aaagtaagca tcctgagaga gaatttcctc atcgacacct
     5281 ctacccactg gggtaaaagg ttcaagcttc agactacagt ctaaatcttt tgctattttg
     5341 agaagatcct ccatatggta gacattcgca ttggttattg tcgatcgaat accaaacttc
     5401 aaacctcttt ccttcaatag catagccccg gagatagcat cttcgtaaga tccctctcca
     5461 tttgcttttg gcctttggcg attttgaatc tctgcaggcc catctatcga gaccgttagt
     5521 ctgaaatcct tttctgctaa ccaatctgca atttctgatg ttattaaggt tccattggta
     5581 actacagaaa aatccttttt acctttccac ttagaattga catattcaac tgccttcttc
     5641 ataagtttaa aggcaacaaa aggttctcct ccaccatgga aagttagttg aaatttctct
     5701 tttcgttctt caagtaaatt tctcattgtt tcatcaattg cactttttgc cgtttcccaa
     5761 gacatggttt tatgtagctt tcccgcttcg gcatagcaat aaatacaacg aagattacaa
     5821 tcagaagtta acgagagcac aagtcgcatt ggtttaaaag gtctttcgac tcgattcacc
     5881 atagctttct tttcctgtac agctctctcc ataagtaagg attcgatcgt tccatccttt
     5941 tgacattttc ttatttcttt aattccgaga tcaaaaacct caaagacaat cctacgaaga
     6001 ggagagaata tgagattcct ctgatcatca gaaattagga aaaagtcatc acacaaaatt
     6061 tcttgcgaaa ttattttcat ttcaatctcc tctctgactg aatatttggt tttaaaagaa
     6121 catgatacga cttatagtat ctatctgatt atatccattt ttgctttgta taacaatact
     6181 tatccacatc aacataacaa ttttaccgta gacccgtgca ctatatttta aaacccttat
     6241 gaggattgag taattttgac tctaaaaagt gaaagtttca aaagtaaggc ctggtagatg
     6301 aaaagagtca aaaaggatga ggttttaaga cctcttgttc caaagataga atgttgattt
     6361 ggtacaagaa atacttttca gttaaaattc acaatatatc tttttcccag aagataatac
     6421 ccttgtattt cccctagatg gaagacatat ttgaggagca gtatgaccaa aatccatatt
     6481 ctgtacaact ggaatatttt tgttatattt tctaactgtt tctaacacta tttctctctg
     6541 tcccctctta tactcatctt tctgttcatc tgtattctgt ttatcaaatt cccaagcttt
     6601 tggtctacct accaacaaac cctgtatatt acccaatatt cccctctcac ccaatgctct
     6661 taaaaccctt gcaacataat tatgagaagg catttcttct gaggtttcaa gatacataat
     6721 aacgttctta aaatcttcta aacttggaat ctctacatta tgtcttaaaa gctcatctat
     6781 agattcaaga caacctcccc acaaaattcc ttcaccatct ttttct
//
LOCUS       DAOQWP010000027         6624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_11957_length_6624_cov_8.444817, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000027 DAOQWP010000000
VERSION     DAOQWP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11957_length_6624_cov_8.444817"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PKI16_03275"
     CDS             complement(<1..1391)
                     /locus_tag="PKI16_03275"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53469.1"
                     /translation="MENEPKIVTQVSIEDINNLLEDQVARRILVKESHYIFFHFYFSQ
                     YTKYKTAPFQKEMFKITEDPNIRMAVIVAFRGSAKSTIMTMSYPIWAILGKQQKKCVV
                     ILSQTQQQAKIHLANIKRELESNELLRKDLGPFQESSDEWGSFSLVIPNFNAKIVAAS
                     SEQSIRGLRHGAYRPDLIICDDVEDLTSTKTKESRDRTYSWFTGEIVPLGEKDTRIIM
                     VGNLLHEDSLLMRLRKNILEDSSNALYREYPVVNDRDEILWKGRYPTMLDLEIEKKLN
                     GDDKAWQREYMLKIIADEDQIVRREWIQYYDEIPIDRNDHRHTLIGVDLAISSSNYAD
                     YTAIVVGHVFGNEETLRIYIEAFPVNQRLDFPSATDKIKEVARVAEKYHRHPKVYIED
                     VGYQRAVIQQLVRQNVSAEGFPVNGQDKRARLDSVTHMIRSGKVLFPKKGTEDLIQQL
                     VYFGVEKHDDLVDA"
     gene            complement(1381..1776)
                     /locus_tag="PKI16_03280"
     CDS             complement(1381..1776)
                     /locus_tag="PKI16_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phBC6A51 family helix-turn-helix protein"
                     /protein_id="HNR53470.1"
                     /translation="MKTLNKKAIVEQLRKTPVIQVTCEKMGVSRATFYRWKKEDPEFA
                     DEAETALQEGLHLINDMAESQLISAIREGNLTGIIFWLKNHHRNYSPKLEVTTTNKDL
                     PLTDEQKELIRKSLAMAFEQEGKEVQDGE"
     gene            complement(1773..3104)
                     /locus_tag="PKI16_03285"
     CDS             complement(1773..3104)
                     /locus_tag="PKI16_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HNR53471.1"
                     /translation="MKHPQISTIQVEIKDLIKADYNPRKWDEKALNDLKTSIKKFGLV
                     DPIIINKAENRKNVVIGGHMRIEAAKALGIATIPAIAINIPEIEREKELNLRLNKNHG
                     EFDFKMMTDFAEDFLADIGFTSEELDQIFEWEDDPETFDLDYELEKLNIERIVVKPGD
                     VWQLGEHKLMCGDSTNPKDVEKLMNGEQADMCFTDPPYILDYLHGKKKNGKATEGFGY
                     KRDRRYIGTDVLPDDFTELWMKNIADIAKKDFHIIVYENWKNIRIIWDELEKYWKVKN
                     MLVWHIPNRNQGYAATYKFFSKYDIAIVSSSNEKLEDLYVETEDELLQNEYETAMYAV
                     SGKPAWEGYKKGKKFQPTDFIEYKASDEKSSGQGIVFGTKPVEIIIPYIKVLTKRGDL
                     VVEPFGGSGSTLMACEKMKRKCYTMEKCDIYAEVIKNRWEKLTKMKAVKIS"
     gene            complement(3184..3906)
                     /locus_tag="PKI16_03290"
     CDS             complement(3184..3906)
                     /locus_tag="PKI16_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53472.1"
                     /translation="MNDWSQTIENIKIIGESVQKQVDLINKSMQPVLEAVAKVQEFSK
                     RMAEIFSKVIYVTPEVYKFLLSHNVQKSPEDIDKGLEEGTLTEEDLYTIFTRDDSKEK
                     ALSFRVTKDMANAIKKILANSPQYMSLETDKIVFDKDEPSLTINGSKLHINPATPRYD
                     LCKYMFKGRNMKKKYWELEDLVGVLGGDYYSGDTDWYEIIYRKYRAFNTFIYEKIGYE
                     NFFVVENTRFTINPIYIYLFNS"
     gene            3977..4501
                     /locus_tag="PKI16_03295"
     CDS             3977..4501
                     /locus_tag="PKI16_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53473.1"
                     /translation="MAISDKSLKGFKRIFEEKSGREWSDDEVRDAAERLVRFYSILFD
                     IYKEEKLKEKKLEEFPNGYHLEDRDGQYHCRICYRYIEGKDTWYDKYGLKCMDCQRNI
                     DNGVVPGEICKDDSLWFKGWQLKSDLGIHPQTAKKLVREGKLKVRELKDEEGKTYFRV
                     YLVSENKEFLKTVK"
     gene            4563..5693
                     /locus_tag="PKI16_03300"
     CDS             4563..5693
                     /locus_tag="PKI16_03300"
                     /inference="COORDINATES: protein motif:HMM:NF025388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HNR53474.1"
                     /translation="MSEKVKNHFVPKFYLKQFVNSDLISLYHIGQDAYIPDIPAEDQA
                     QKKRLYGDNSELEDAFGELETEVAPILKNIIKKNKIPPKSSMNYFSLMNFTILQNQRT
                     FKAIYQTDKGIEQMLKKYLEDIPDFKDIDLDKYEIRTKNAFALATGSAIPIAIESIDL
                     EPVLIVNNTKYPFIVSDNPCIKYNLFAEHIRWHNDGRGFGTTGLQIFLPLNERLLIAF
                     FDKKVYKTNTKGKKKTILNATKEDVINLNKLQFVCADECLYYKNRKNIKEVISQIKEA
                     EGLRKDFIPSTQKLHGQDGYLLIHSEAQLKAMLQLSFFQIKKEYKNFSREGLSLGGLK
                     RESFNDFYIKTFDMALRELDPKKVTMKLFEILTEKQKKYGNQ"
     gene            5680..6195
                     /locus_tag="PKI16_03305"
     CDS             5680..6195
                     /locus_tag="PKI16_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53475.1"
                     /translation="MEINKKQIIEINIALKFLELYNEEKSLSIKYIREGDSDKREPDI
                     ICSDEYSIEITSFYDNQSQAKSYWEDIKGITNPYQKRNIKLEPEEQLVGAIGSKLTKL
                     EQGLYGGVDQTRIFLLCYSESPLFDTNEANKIKSNYQPFRQDNFFTKYFFETWLMWRQ
                     GGDAYGILKLE"
BASE COUNT         2068 a         1243 c         1057 g         2256 t
ORIGIN      
        1 gcatctacca gatcatcatg cttctctaca ccgaagtata ctaactgctg tattagatcc
       61 tctgtaccct tcttcgggaa tagaaccttt cctgatctaa tcatatgggt tacagaatct
      121 aacctagccc ttttgtcttg tccattaaca ggaaaaccct ctgcagagac attctgcctt
      181 acaagttgtt gaatcactgc cctttgatat ccgacatctt caatatacac ctttggatgt
      241 ctatgatact tctctgcaac ccttgccact tcttttatct tatctgtagc actagggaaa
      301 tctaatctct gattcactgg gaatgcttct atatatattc ttaacgtttc ctcattacca
      361 aagacatgcc caacaactat ggctgtataa tctgcataat tacttgagga aatagccaaa
      421 tctactccga ttagggtatg cctatggtca tttctatcta ttggaatctc atcatagtac
      481 tgtatccatt ctcttctaac tatctgatcc tcatctgcta ttatcttaag catatactct
      541 ctttgccatg ccttatcatc tccattcagc ttcttctcta tttccaaatc caacatagtc
      601 ggatatctac ccttccatag gatttcatcc ctatcattta caacaggata ctctctgtac
      661 aatgcattac tagaatcctc taggatattc tttcttagcc tcattagtaa tgaatcctca
      721 tgtaggaggt ttccaaccat tataatcctt gtatcctttt ctccaagtgg tactatctca
      781 cctgtaaacc agctatatgt tctatctctg ctttcttttg tttttgtcga cgttaaatct
      841 tctacgtcat cacagattat taaatcaggc ctatatgctc catgtcttaa acctctgata
      901 ctctgttcac tagatgctgc tactatcttt gcattaaaat taggaataac taaagagaat
      961 gacccccact catctgatga ctcttggaaa ggtcctaggt cttttctaag taactcatta
     1021 gactccaact ctctctttat gttagctaag tgtatctttg cttgttgctg ggtctgagag
     1081 agtataacta cacatttctt ttgctgttta ccaagtatag cccatatagg gtatgacata
     1141 gtcataatag tagatttagc agaacctctg aaggcaacaa ttactgccat cctaatgtta
     1201 ggatcttccg tgattttaaa catctccttt tggaatggag ctgttttgta cttagtatat
     1261 tgactaaaat agaagtgaaa aaatatatag tgactctctt tgacaagtat tctccttgct
     1321 acttgatcct caaggagatt gttaatatcc tctatactta cctgtgtcac gatcttgggt
     1381 tcattctcca tcttgcacct cctttccctc ctgctcaaat gccatagcaa gagatttacg
     1441 tattaactct ttttgttcat ctgttagagg tagatctttg ttagtagtag ttacttcaag
     1501 ctttggagag tagtttctat gatgattttt taaccagaag ataatacctg ttaaattccc
     1561 ttctcgaatg gcagagatca attgactctc tgccatatca tttattaagt gtaatccttc
     1621 ttgcaaagcc gtttcggctt cgtctgcaaa ttctggatct tcctttttcc atctgtagaa
     1681 tgtcgctcta gatactccca tcttctcaca ggtaacctgt atgacaggag tttttcgtag
     1741 ttgttcaaca atagctttct tatttaaagt cttcatgata tttttacagc tttcatttta
     1801 gttagtttct cccatctgtt tttaatgacc tctgcgtata tgtcacattt ttccattgtg
     1861 tagcatttcc ttttcatctt ctcgcatgcc attagtgttg atccactccc tccaaatggt
     1921 tcaactacta agtctcctct tttggtaagt actttgatgt atgggattat tatttctact
     1981 ggcttagttc cgaatacaat accttgccca gagcttttct catcagatgc tttgtattca
     2041 ataaaatctg ttggctggaa tttctttcct ttcttgtatc cttcccaagc aggcttacca
     2101 gagacagcat acattgctgt ctcgtactca ttttgtaaca gttcatcctc cgtttctaca
     2161 taaagatctt caagcttttc attggaggaa cttactatag caatgtcata ctttgagaaa
     2221 aatttataag ttgcagcata tccctgattt ctatttggta tatgccatac caacatattc
     2281 tttactttcc aatatttctc taattcatcc catattattc ggatattttt ccaattttca
     2341 tatacaatta tatggaagtc tttcttcgct atatcagcga tgtttttcat ccatagttct
     2401 gtaaaatcgt ctggaagtac atcggtacct atatatcttc tgtctctttt atatccaaac
     2461 ccttctgttg ccttaccatt tttcttcttg ccatgtaaat agtctagaat gtatggagga
     2521 tcggtgaaac acatatctgc ttgctctcca ttcattaact tttcaacatc tttaggattt
     2581 gtagagtctc cacacattag tttgtgttca cctaattgcc atacgtctcc aggtttaact
     2641 actatccttt ctatattgag tttttctaac tcataatcca aatcaaatgt ctctggatca
     2701 tcttcccatt caaatatttg gtctagttcc tcagaggtaa atccaatatc tgctaggaag
     2761 tcttcagcaa aatcagtcat cattttaaaa tcgaactcgc cgtggttttt atttaacctc
     2821 aggttaagtt ctttttctct ctctatctca gggatattta ttgctattgc aggtatcgtt
     2881 gctataccta gagctttagc agcttctatt ctcatgtggc cacctattac aacattcttt
     2941 ctattctcag ccttattgat aatgattgga tcaactaatc caaacttctt aatgctggtt
     3001 tttagatcat ttagagcctt ttcatcccat ttccttgggt tgtagtcggc tttgataagg
     3061 tcctttattt ctacttgtat agtagatatt tgtggatgct tcatattaaa tatatataaa
     3121 aatttaacta gagtattcct actcttgtta aatcatatag aactggaaat ggcccaaagt
     3181 ggcctaacta ttaaaaagat agatatagat agggtttatt gtaaatcttg tattctcaac
     3241 tacaaagaaa ttttcataac caatcttctc atatataaat gtgttaaaag ctctatattt
     3301 acgatatata atctcatacc aatcagtatc tcctgagtag taatcacctc ctaatactcc
     3361 gactaaatct tccaattccc aatatttctt tttcatgttt ctgcctttaa acatatactt
     3421 acacaaatcg tatctcggag tagctggatt aatatgtagt ttggatccat taatggttaa
     3481 cgatggttca tccttatcaa agactatttt atctgtctct aaagacatat attgcgggct
     3541 atttgcaagt atttttttga ttgcattggc catatcttta gtaacacgaa atgacagagc
     3601 cttttcttta ctatcatctc ttgtaaagat tgtgtaaaga tcttcttccg taagagtacc
     3661 ttcttccaaa cccttatcta tatcttcagg agatttctga acattgtggc ttagtaaaaa
     3721 cttataaact tcaggtgtaa catagattac ttttgagaaa atctctgcca ttctcttact
     3781 aaattcctga acttttgcga cggcttctaa gacaggttgc atagacttat ttattaggtc
     3841 tacctgcttt tggacactct cccctattat ctttatgttt tctattgttt gactccagtc
     3901 attcatattg ctggtctcct tctcaccttt gaactataat atattactgc ctaagtataa
     3961 tatttgttgt actaatatgg caataagcga caaatcactg aaagggttta agaggatttt
     4021 tgaagaaaag tctggaagag aatggtccga tgatgaagta agagatgcag cagaaaggtt
     4081 agtgagattc tattccatcc tcttcgatat atacaaagaa gagaagctta aggagaagaa
     4141 gttagaagaa tttcccaatg gatatcattt agaagataga gatggtcaat accattgcag
     4201 aatctgttat agatacattg aaggtaagga tacttggtat gacaaatatg gattaaagtg
     4261 tatggattgt cagaggaata tagataatgg agttgtacct ggagagatat gtaaggatga
     4321 tagtttatgg tttaagggtt ggcagttaaa gagtgattta ggaatacatc ctcagacagc
     4381 taaaaagtta gtaagagagg ggaaattaaa agtaagagag ttaaaggatg aagaaggaaa
     4441 aacatatttc agggtgtatt tggttagtga gaataaagag tttttaaaaa cagttaaatg
     4501 aaaagagaag aataggacca accctattat ggcagataaa gataagatta ttttttaaaa
     4561 gaatgtcaga gaaagtaaaa aaccactttg ttccaaagtt ttacctcaag caatttgtaa
     4621 actcagatct tataagccta tatcatattg ggcaggatgc atacatacct gacataccag
     4681 cggaagatca agctcaaaag aagcgcttat atggtgataa ttcagagtta gaagacgctt
     4741 ttggggagtt ggagacggaa gtcgcaccaa tactgaaaaa tattattaag aaaaataaaa
     4801 taccccctaa atctagcatg aactattttt ctctcatgaa ttttacaata cttcaaaatc
     4861 agagaacctt taaagctata tatcaaactg acaaaggcat tgagcagatg cttaagaaat
     4921 acttagaaga tattccagac tttaaagata ttgatcttga taaatatgaa atacgaacga
     4981 agaatgcctt tgcactggca actggtagcg caatacctat tgctatagaa tctatagacc
     5041 tcgagcctgt acttattgtg aataacacaa aatacccatt tatagtatct gataatccct
     5101 gcattaaata caatctattt gcagaacaca ttcgttggca caatgacgga agaggtttcg
     5161 ggacaactgg acttcagata ttcttgccat taaatgaaag attactaata gccttctttg
     5221 ataaaaaagt atacaaaaca aacactaaag gaaagaagaa aactatctta aatgcgacaa
     5281 aagaagacgt aatcaatctc aacaagttgc aatttgtttg tgcagatgaa tgtctttatt
     5341 ataagaacag gaaaaacatt aaagaagtga tctctcagat taaagaagct gaaggtctta
     5401 gaaaagattt tatcccaagt acacaaaagc ttcatggtca ggatgggtat ctactcatac
     5461 actcggaagc ccaactgaaa gcaatgttac agttatcatt ttttcaaatt aaaaaagagt
     5521 ataaaaattt ctctagagaa ggattgtctc taggtggact taagcgagaa tccttcaatg
     5581 atttttatat taaaactttt gatatggcat taagagagct tgatccaaag aaagtgacaa
     5641 tgaaactttt tgaaatacta actgaaaagc aaaagaagta tggaaatcaa taaaaaacag
     5701 attatagaaa taaatatagc gctaaagttc cttgaacttt ataacgaaga gaaatcctta
     5761 tccataaagt atataagaga aggagattca gacaagaggg aaccggatat tatctgctca
     5821 gacgaatact ctattgaaat tacaagcttc tatgacaatc aatcacaggc caaaagctac
     5881 tgggaagata ttaaaggaat tacaaatcct tatcaaaaaa ggaatataaa gttagaacca
     5941 gaggaacaat tagtaggtgc aataggaagt aaactcacta aactcgagca gggtttatat
     6001 ggaggtgttg atcaaactag aatattttta ttgtgctatt ctgagagtcc attatttgac
     6061 actaacgaag caaacaaaat taagtccaat tatcaaccgt ttaggcaaga taacttcttt
     6121 accaaatact tctttgaaac ttggctcatg tggaggcaag ggggagatgc ttatgggata
     6181 ctaaagttgg aataagacat aaatttcatc cccttttata ctgtcttatt tgtctcattt
     6241 taggttccag actcatatgt tccgccagcc catagttgtt ccaatgagac ccgtgtgcta
     6301 ccacttagaa acgttgatgt tgcaccacaa ttaaactgtc aatttttggc attttcaaaa
     6361 attaggcctg gtgtatgaaa atacccaaaa acagaccaaa ttaattaccc ctttgttcga
     6421 agttagaatg ttgatttaac acaataataa tcagacaaca aaaatcttct ttccgttcca
     6481 gtcctgtttc tgttaactat attatgttga taatcaatta ttacctttac aaataccttg
     6541 tcaattaact tgacaattat ttgacagatc catataatgg attatgataa atactaagta
     6601 caaattaagt aaaaaactct taga
//
LOCUS       DAOQWP010000028         6197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_12971_length_6197_cov_6.293064, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000028 DAOQWP010000000
VERSION     DAOQWP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12971_length_6197_cov_6.293064"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..138)
                     /locus_tag="PKI16_03310"
     tRNA            complement(62..138)
                     /locus_tag="PKI16_03310"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Asp,seq:gtc)
     gene            210..1106
                     /locus_tag="PKI16_03315"
     CDS             210..1106
                     /locus_tag="PKI16_03315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNR53476.1"
                     /translation="MNLYNNILSSFIGITVLEIEPSGKVKNIVLDTRNCLHKKKPKTV
                     FDIFSKEEKFRVKRVLELGGAQRKRYVELDKKYSKGEFVDIAVDWSGSKLYMYIQFFE
                     SNREKEVAFDRYIENLANLSEKDPLTNVLNRNGFWQKTKQLLLLSDPDKRIGIIYLDV
                     DRLKEINDKHGHDVGDITILEISQTILTSLRDRDIVTRVGGDEFVIVVEELSGKRSTA
                     YGLAKRLLKDIAEKKGISSTTISAGVHVTKVKTFSPYFTKEPQLKKEWDKAIKQADDA
                     LYVSKQAGGNCVSVSSEYSKYY"
     gene            complement(1103..1426)
                     /gene="trxA"
                     /locus_tag="PKI16_03320"
     CDS             complement(1103..1426)
                     /gene="trxA"
                     /locus_tag="PKI16_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006637910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNR53477.1"
                     /translation="MSVVHVTDENFENEVLKSDGLVLTDFWAPWCGPCRMLGPILEEL
                     EKEMGEKVKVCKVNVDEEGTVAQKYGIMSIPAVFLFKGGEVVETMIGLAPKESFEEAI
                     KKHLD"
     gene            1496..2575
                     /locus_tag="PKI16_03325"
     CDS             1496..2575
                     /locus_tag="PKI16_03325"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNR53478.1"
                     /translation="MLKIDIYKNRQQAILKAIDTLKNGGLIIYPTETCYGLGGDATNP
                     KALAKLLSYKKYRGSKPISIAVSDKQMAKKYVELNEMANNLYENYLPGPITVISMSKG
                     VLTSPVVSPRGAVGVRIPAYAFTQELISKYGKPITATSANVSYRSTPYSIDKLVKDLP
                     KKSLELIDLFLDAGQLPPNPPSTVLDTTMNELSVLRQGSIQFANALVKNKKIFARETK
                     STQETISLAKEFSDKYLKNGIPSVVALSGELGAGKTQFTKGIGQSLGVEGIVNSPTYT
                     IINEYKYDGKILAHMDTWRLEKGELEKSGLIENLEAGNIVAIEWADKFFEEISALCDN
                     MDIPMYKVVIKHVSEQERSIEIYEG"
     gene            2565..3626
                     /gene="tsaD"
                     /locus_tag="PKI16_03330"
     CDS             2565..3626
                     /gene="tsaD"
                     /locus_tag="PKI16_03330"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNR53479.1"
                     /translation="MKDSQPNIIAFDTSCDDTSVAVLQGRRILSSEVSSQVEIHSQWG
                     GVVPDIARREHEKNIPLVYEEALRKSKLKIEDMDYVVATYGPGLAIDLEIGLQFAKDL
                     AIKYNKPFVPVNHMEGHLLSGLLLNSKGAGISNEEELFPALGLLISGKHTELIYSPKI
                     GQYKKLGETLDDAVGEAFDKVARMLNFGYPGGAIICELAKKGKGGNISFSVPMKNSKD
                     LNFSYSGIKTAALYKTKELRESGVKDSAWVYDFCRGFLDSIIESLIIKLELAIEQNSD
                     IKSLFVGGGVFNSEEIVRKIGAVAKKYGLEYVYPKGEYRGDNAGMVGIAGYYKIQRKE
                     YITSKEDIQKIDREPRLSL"
     gene            3670..4722
                     /locus_tag="PKI16_03335"
     CDS             3670..4722
                     /locus_tag="PKI16_03335"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNR53480.1"
                     /translation="MIQSDERKQKYWLKKRDIKLEEAYAKIAPNIPLVNVSLKNLFLK
                     KCGEYYIFLLSKKSGIKIAERWDEFEIMRMFGKGLNPTSLKTALNNIDFLVENIKVQG
                     DIVRILEYGPGAGWSTIMLHNQLKKKFPDKKIELISVDISPHSIVATQNTLDYYQVPW
                     QTSLKGDSVKNYSEKEGYVTIVLDDFVEFTKKQPNDYFDGFFSSHGTAYLSEDEYVSL
                     LGEMKLKGKKGSVFVADSLDPLYTVKLDTLHLLFCSLFPSVTKNMREYVYGKRLYSNS
                     KYFPRGEIVKKLIKVNNEESLLFYRWNHYLLSRFKILYIVQMLKSMKITTDVIEEYRE
                     DVYPSYLVNKLIRGRS"
     gene            4802..>6197
                     /locus_tag="PKI16_03340"
     CDS             4802..>6197
                     /locus_tag="PKI16_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53481.1"
                     /translation="MTDLIGIINLLSGITQMGLGFFVLFSDFRNRVKRYYFFSVFFLG
                     LWSLCIFFYSNPILFDTTIWLKIVYTAAYCMTLGLILFARVYPEELKKKFKTFFLIIS
                     SYMFVMTILLWFTNLVVVDTYNLVGEFNSIAKMGSLYFFYGLPEFITAVYIVGYYIRQ
                     TKILKGIEKRQVQLYVIGGIVMLIPVFLFDFIFPLAYGNTSFYKYGTIGNSIWTIIVG
                     YSILTTRFLDTRVLVGSFIQMILKAVSVLALFLLTIFLVVPLWRIDFSFIGIVKLTAI
                     SLVVTLILGGIFKWIDEFLSEKIVYTRYHPITSLKIFQSKNAETVKMEDISENLLTLI
                     SNSFDTNLSSVILLDKKDNVLVKQVKGKWEAGVEDIVNTLKVWRKLNSNRVLIYSELV
                     KDKRAGKRIIDSKRGAILSFMKKNGIEIIFSLKEFEKFNGAVIIGEKYNKDSYTVGDI
                     DFLESVMQSTHMALV"
BASE COUNT         2180 a          991 c         1196 g         1830 t
ORIGIN      
        1 actcaatccc cataagggtt ttaaaagata gtggacgggt cttgttggaa catgttgaaa
       61 atggcggaat ggacgggact cgaacccgca accttctcag tgacagtgag atgctctaac
      121 caattgagct accactccat atacatcgtt agaagtatat caaacaatat taattgagta
      181 aatataccca tatatgatat atttatccta tgaacctcta caacaacatt ctctcttcat
      241 tcataggtat taccgtacta gaaatagaac catctggtaa agttaaaaac attgtcctag
      301 atactagaaa ttgtttacac aaaaagaaac ccaaaacagt cttcgacata tttagtaaag
      361 aagaaaaatt cagagttaaa agagtcctag aactaggagg agcacaaagg aaaaggtatg
      421 tggaattaga taaaaaatat tccaaaggag aatttgtaga cattgcagta gattggtcag
      481 gaagtaaact ctacatgtac atacaattct ttgaatctaa cagagaaaaa gaagtagcct
      541 ttgacagata tattgaaaac cttgctaacc tctctgagaa agaccctcta accaatgtat
      601 taaacagaaa tggattctgg caaaaaacca agcaactctt actcctttca gacccagaca
      661 agagaattgg tattatctat ctagatgtag atagattaaa agaaattaac gacaaacacg
      721 gacacgatgt aggagacata accatactcg agatatctca aaccatactc acctctttgc
      781 gagacagaga cattgtaact agagtaggtg gagatgaatt cgtaattgtg gttgaagaac
      841 tctctggtaa aagaagtaca gcatacggac ttgctaaaag attactcaaa gatattgcag
      901 agaaaaaagg tatttcctct accaccatat ctgcaggagt acatgttact aaggtaaaaa
      961 cattctcccc atatttcacc aaagagccac agcttaagaa agaatgggac aaagcaatta
     1021 aacaggcaga tgatgccttg tatgtgtcaa aacaagcagg tggtaactgt gtatctgtat
     1081 ctagtgaata ctctaaatat tactaatcta agtgtttctt aatagcctct tcaaaagact
     1141 ccttcggtgc aagacctatc attgtttcta caacctcacc acccttgaaa agaaatactg
     1201 ctggtatact cataatcccg tacttctgtg ctactgtccc ctcctcatct acattaacct
     1261 tacaaacctt taccttctcc cccatctcct tctctaattc ctctaagatt ggtcccaaca
     1321 ttctacaagg accacaccat ggagcccaga aatctgtgag gactaaacca tcactcttaa
     1381 gaacctcatt ctcaaaattt tcatctgtta catgtactac tgacatatat atgttccgtt
     1441 aatttaaaag tattgaaata tgggcattta aatacattcc tagtataatt ataccatgct
     1501 aaagatagat atctacaaaa acaggcaaca agcaatacta aaagccattg acactcttaa
     1561 aaatggtgga cttatcatat acccaactga aacatgctac ggactcggtg gagatgcaac
     1621 taatcctaaa gctcttgcta aactactctc ctacaagaaa tacagaggct ccaaacccat
     1681 atccatagcc gtatctgaca aacaaatggc aaagaagtat gtagagctca acgaaatggc
     1741 aaacaacctt tacgaaaact atctcccagg ccccataaca gtaatctcca tgagcaaagg
     1801 agtacttact agtcctgtgg tatcccctcg tggagcagta ggagtaagaa taccagcata
     1861 tgcctttaca caggaactaa tctctaaata cggtaaaccc ataaccgcaa catccgcaaa
     1921 tgtatcctac agatctacac catacagtat agacaagtta gtaaaagatc ttcctaagaa
     1981 aagcttagaa ttaatagatc tgtttttaga tgctgggcaa ctacctccaa atcctccctc
     2041 tacagtacta gatacaacta tgaatgaact ttctgtatta agacagggct caattcagtt
     2101 tgcaaacgct cttgttaaaa acaaaaagat ttttgctaga gaaaccaaaa gtacccagga
     2161 aaccatatct ctagctaaag aattctcaga caaatatctt aaaaacggta taccatctgt
     2221 tgtagctcta agtggagaac taggagcagg taagactcag tttactaaag gtattggaca
     2281 gagtcttggt gtagagggga tagttaattc tccaacatat accattatca atgagtataa
     2341 atacgatggt aagatacttg cacatatgga tacatggaga ttagaaaagg gggagttaga
     2401 aaaatctgga ttaattgaaa acttagaagc aggaaatatt gtagccatcg aatgggcaga
     2461 caaattcttt gaagaaatat ctgcactatg tgacaatatg gacataccaa tgtacaaggt
     2521 agtaattaaa catgtatctg aacaggaaag gagtatagag atatatgaag gatagccaac
     2581 caaacataat tgcatttgac acaagctgtg acgatacatc cgtagccgta ctacaaggaa
     2641 gaaggatact ttcaagcgag gtatcctctc aagtagaaat tcactctcag tggggtggag
     2701 tagtcccaga tattgctagg cgtgagcatg agaagaatat ccccttggtg tatgaggaag
     2761 cattaaggaa atctaaactt aagattgaag atatggatta tgttgtagct acatatggtc
     2821 cgggtttggc tatagactta gaaataggtc ttcaatttgc aaaagattta gctattaaat
     2881 ataacaaacc atttgtacct gttaaccata tggaaggaca tctactctca ggattgctac
     2941 ttaattctaa gggtgctggt atatctaatg aagaagagct ttttccagca ttgggtctgt
     3001 taatctctgg caaacataca gaactaatat attctcctaa gattgggcag tacaagaaac
     3061 ttggggaaac cctagatgat gcagtagggg aagcattcga caaagtagca agaatgttaa
     3121 acttcgggta tccaggtgga gccataatct gtgaacttgc taagaaggga aagggtggga
     3181 atatatcttt tagtgtacca atgaaaaatt ccaaagattt gaattttagt tactctggta
     3241 taaagacagc agctctgtac aagacaaaag agttaagaga aagtggtgtt aaggatagtg
     3301 cgtgggtata tgatttctgt agaggatttc tagatagtat tattgagtct cttattatta
     3361 aattggaatt agcaattgaa cagaactcag atattaagag tttgtttgtt ggtggaggtg
     3421 tgtttaacag tgaggagatt gtaagaaaga ttggggctgt agcaaagaag tatggtttgg
     3481 aatatgtata tccaaaaggt gagtacagag gagataatgc tggtatggtg ggtatagctg
     3541 ggtattacaa aatacagagg aaagagtata ttactagtaa ggaagatata cagaagatag
     3601 atagggaacc tagattgagt ttgtagtgat aggtatatat aatgaattca taatattaat
     3661 ctgccttata tgatccaaag tgatgaacgg aaacagaagt attggttaaa gaaacgagac
     3721 attaaacttg aagaagcata tgcaaaaata gcccccaata tcccacttgt aaatgtatct
     3781 ctaaaaaatc tttttttaaa aaagtgtggg gaatattata tttttttact tagtaaaaaa
     3841 tctgggataa aaatagctga aagatgggat gaatttgaaa taatgagaat gttcggaaaa
     3901 ggattaaatc ctacctcgtt aaagaccgct ttaaacaata ttgatttctt ggttgagaat
     3961 attaaagtac aaggagatat tgttaggata ttggagtatg gacctggtgc tggatggtca
     4021 accataatgc tacataacca attgaaaaag aaatttccag ataaaaagat agaattaata
     4081 tccgtagata tatctcctca ctctatagtt gctacacaaa acactctaga ttattaccaa
     4141 gttccttggc agacatcttt aaagggggac agtgtaaaga attattcaga gaaggaaggg
     4201 tatgtgacaa ttgttttaga tgattttgta gaatttacaa aaaaacaacc aaatgattat
     4261 tttgatggtt tcttctctag tcatggtact gcctatctgt cagaggatga atatgtatct
     4321 ttgttgggtg aaatgaaatt gaagggaaag aaagggagtg tgtttgttgc tgattcccta
     4381 gatcctctgt ataccgttaa actagatacc ctgcatctac tcttctgttc cctattccca
     4441 tccgtgacaa agaacatgag agagtatgtg tatgggaaga ggttgtacag caattcaaaa
     4501 tattttccaa ggggggagat agtaaaaaag cttattaagg taaataacga agagtcgtta
     4561 ctgttttaca gatggaatca ttatctctta tcaaggttta aaattcttta cattgtacaa
     4621 atgcttaaat caatgaaaat tactacggat gttatagaag agtacagaga ggatgtatat
     4681 ccatcttatc ttgttaacaa gttaataaga gggaggagtt aaacccatgg aaggagatag
     4741 gaaatcctcc aaagtgtcct ttgtatatat gtaattgtgc tcttagatta gataagtagc
     4801 catgacagat ttaattggaa ttataaatct actctcaggc ataacccaaa tgggacttgg
     4861 tttctttgta cttttttctg atttcagaaa cagggttaag agatattatt tcttctctgt
     4921 atttttcttg gggttatggt ctctgtgtat attcttttac tcaaacccaa tccttttcga
     4981 tactacgata tggcttaaaa ttgtatatac agctgcctac tgtatgacgc tgggtttaat
     5041 tttgtttgca agagtatatc cagaggagtt aaagaaaaag tttaaaacat ttttcctaat
     5101 tattagctcg tacatgtttg ttatgactat actcctatgg tttacaaatc ttgttgtagt
     5161 agacacatac aatcttgtag gagaatttaa tagtattgca aaaatgggct cgctgtattt
     5221 tttctatggt ctaccagaat tcataactgc ggtatatatc gttgggtact acataaggca
     5281 gactaagatt ttaaaaggta tagaaaaaag acaggtacaa ctatatgtaa ttgggggaat
     5341 agtaatgctt attcctgttt ttctttttga tttcatattt cctttggcat acggaaatac
     5401 gagcttttat aaatatggaa caattggtaa ttcgatttgg accataatcg ttggatattc
     5461 aatcttaacg accagattcc tagataccag agtgttggta ggttcattta tacaaatgat
     5521 actaaaggca gtatcggtat tagctctttt tttgctaact attttcttag tagtaccatt
     5581 gtggagaata gatttttcct ttattggtat tgttaagcta acggctattt cccttgttgt
     5641 taccctaata cttggtggta tatttaagtg gattgatgaa tttctttcag agaaaattgt
     5701 atatacaaga tatcatccaa taaccagttt aaagattttt cagagtaaaa atgctgagac
     5761 tgtaaaaatg gaagatatct ctgagaactt acttacatta atttcaaatt ctttcgacac
     5821 taatctttct tcagtaatac tgttggacaa aaaagacaat gtacttgtta aacaggtaaa
     5881 gggaaagtgg gaggctggag tagaggatat tgttaatact ttaaaggttt ggaggaagtt
     5941 aaacagcaac agggtattaa tatattcaga gttagtaaaa gacaaacgag caggaaaaag
     6001 aatcatagat agtaaaagag gggcaattct ttcctttatg aagaagaatg gtatagaaat
     6061 aatcttctct cttaaagaat ttgaaaaatt taatggtgca gtaataatag gggaaaaata
     6121 caacaaagat tcatatactg ttggagatat agattttctt gaaagtgtta tgcaaagtac
     6181 acatatggct ttagtta
//
LOCUS       DAOQWP010000029         5328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_15741_length_5328_cov_6.065428, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000029 DAOQWP010000000
VERSION     DAOQWP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15741_length_5328_cov_6.065428"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKI16_03345"
     CDS             <1..638
                     /locus_tag="PKI16_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53482.1"
                     /translation="VVIASYYTNDCGEKCNNLDNSNPDKSNNVSKKFNRMYYSYPGQK
                     DKDVISGDISIEGECKDVLPANIDSAIPGCFDTRQFLLGLFGSVFPSSDWTNCSKDEE
                     VCVNAEDIVLKISPMFEDTNRFTNTRNKTAMDPVSASTYKSVYVMTPCRINVAGKSVN
                     VKCAWDMSYIFEERKAAEFDDVGGSDTPTHEEYVRFLEKESSTRQDTPLSM"
     gene            662..2134
                     /locus_tag="PKI16_03350"
     CDS             662..2134
                     /locus_tag="PKI16_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53483.1"
                     /translation="MSFVKTGYRIFSKLEEIFKRKSFKTVFIAISSMILFFGVMNVFA
                     QETEVPKLNVDWKGMKAYYNLASGTDKDEDNMTSFSGVSATNTAWTILSTMAPGLTEG
                     GDEIQKGESIPADMQGGLLGTADKFVNYAYTNQPSVNLVAHLGNEWVPGYKESTSGLY
                     AADKQSGYDELMDSGISNIWAKFRDLAYLFFVVVMIVIGFMIMFRSKLGGQTLVTLGN
                     TIPNVIIGLILVTFSFAIAGFIIDVGGLITNFLASLFNGDLVSIENIGNLMKSVFTSD
                     TKKVSGIVAGSGGIAALGGWGLGSLATAIGGSAVLAILGPAFVGVGLIAILLVLALLG
                     IVFVGVIKLLITLYKAYFGILLGVAIGPLQIMIGTIPGQNHMTMNWLLGIVRNVLVFP
                     VVFFIVNAPNLLLGSGDIILNFPEKLTYADPNSTGMDINATSGFLIFILRVFVIYLAA
                     QAPKYIDAFIPNTSSKAAGDAGAAAKMSLSKIPLVGGLFK"
     gene            2148..4958
                     /locus_tag="PKI16_03355"
     CDS             2148..4958
                     /locus_tag="PKI16_03355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNR53484.1"
                     /translation="MPKPRKEENPDALGQIFDFIFKEAEKPPDKRRPVKVTGISGQNE
                     LTDALAAALEKPGLFMSDQILDVFNEALDLEVARIKANEQGSATVKFSANNLISMLDD
                     PNAFIEKGFTSAKTFRKQMRAQYLGKTMQHFVAEGWAKKYNLDLDAQEAIRGYFGARH
                     LDKTLSKEERAKSYDASNAIGASVEGRFFRGFPGSGELNLTSEKEHLTNRAFELLGRQ
                     YFGASNWQQLSDDQKRSFEKVHIDWSAMSSADKKKFMAANNGMDAVEKYITSNFNPTT
                     ANSLFNNYRNARTSLGLAKKEDTFDVVNRDRYLDLEVKNLDDRITQLRQYMNTPNLSN
                     REVTNLQVGIQRLEQAKVIVGGQLLDPESIHIAQKKIDSAVDSFRLQMDQARAQGDMD
                     SVKLYRQQIKDLRTGKRELNSIMLWGRVGQIEGYINSLNGIYKDGNFIKSYLDGTFFD
                     PNRNNLFRPTADRDVKFSFGEKNGEIGIHVAAPTKNALLKTYNNTLTNLYYLTPKSIM
                     KSLFVNGEGFVYLTYMKKLGLEGSLKDILHNKKFEISIEDLLSTGNAQTYLNNILSGT
                     NRQLTQQEIEKYTKELKALMRRSRTAKIFSFTQRTRDKIKKFFDDKIFREMRQKIYDK
                     LMAQKAFQVAEEVLGQWLAKGGIQVFVKGLVIGITNAVGIVTTGGLANFAITIVTALL
                     VDVMYGIIKVLAMIVIFAFVGIFAMAFMLGSKSTYKHALNSYAYTTVVPGEVVTNPNF
                     RGLYPIDDGEIPGELHPFSGGSLPDGVQCLIGTGGFRCSQGPFSSYSHSKVAAIDITD
                     GVRTFYAPTFCDGSNCVVKTVAEVNCTRCDIPSMPGCHQFPAGGMVIFDATYGSTTYT
                     FKLIHVYKDVSVGQKLSSGEAVADIIQAGPDHERIEKCSSGPHLHLEVKANGSSVNPM
                     DVMTGPTSSGGFACNISACPVN"
     gene            4960..>5328
                     /locus_tag="PKI16_03360"
     CDS             4960..>5328
                     /locus_tag="PKI16_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53485.1"
                     /translation="MNKKTKTILIISFSLLFVGVCVGVYFLLTIPPKTYPKDPIEQTS
                     LYTTSQEINFYSEDLESKDYPLYGVSSEVMLKEVEAFVSTIAPQLKKISNDEGAYYEW
                     GFKEDSVTYNLTQNALMFSFA"
BASE COUNT         1832 a          798 c         1112 g         1586 t
ORIGIN      
        1 aagtagtaat cgcaagctat tatacgaatg attgtggtga gaaatgtaat aatttagaca
       61 atagtaatcc agataaatcg aataatgtaa gtaaaaaatt caacagaatg tattatagct
      121 atccaggaca aaaagataag gatgtaataa gtggagatat cagtatagaa ggtgagtgta
      181 aggatgtttt acctgcaaat attgatagtg caattccagg gtgttttgat acacggcagt
      241 ttcttcttgg tcttttcggc tctgtatttc catcttctga ttggacaaat tgcagtaaag
      301 acgaagaagt ttgtgttaat gctgaggata ttgtattaaa gataagtcca atgtttgaag
      361 acacaaatag atttacaaat actagaaata aaacagcgat ggaccctgta tcggctagta
      421 cttataaatc tgtttatgta atgaccccct gtagaattaa tgtcgcaggt aaatcagtta
      481 atgtaaaatg cgcatgggat atgtcatata tatttgaaga aagaaaggct gctgaatttg
      541 atgatgttgg aggatctgat acaccaaccc atgaagagta tgttagattc ttagagaaag
      601 agagttcaac aagacaagat actccactta gtatgtaatt taaattaata attaaaataa
      661 tatgagtttt gtaaaaacag gatacagaat cttctccaaa ctagaagaga tatttaaaag
      721 aaagagtttt aaaacagttt tcattgctat ttcctccatg attctctttt ttggagtaat
      781 gaatgttttt gctcaagaaa cagaagtacc aaagttgaat gtggattgga aaggcatgaa
      841 ggcatactac aatcttgcta gtggaactga caaggatgag gataacatga ctagcttctc
      901 tggagtgtca gctacaaaca cagcttggac aattctctca actatggcac ctgggctaac
      961 agagggagga gatgagatac aaaagggaga atctattcct gcggatatgc aaggtggact
     1021 acttggtacg gcagataagt ttgttaatta tgcatatacc aaccaaccga gtgttaattt
     1081 agtagcacac ctaggaaatg agtgggttcc aggatacaag gaaagtacat caggattgta
     1141 tgctgcagac aaacagagcg gatatgatga acttatggat agtggtatat ccaatatatg
     1201 ggcgaaattt agagatttag catatttatt ctttgtagta gtaatgattg ttattggatt
     1261 tatgattatg tttagatcca agttaggtgg gcagacattg gtaactttgg gaaatacaat
     1321 acctaatgta attattggat taatacttgt tacatttagt ttcgcaatag ctggattcat
     1381 tattgatgtc ggaggattaa taacaaattt cttagcaagt ttatttaatg gagatcttgt
     1441 atctatagaa aatatcggga atctaatgaa atctgtattt acttctgata ctaaaaaggt
     1501 gtcgggtatt gttgcaggaa gtggagggat agccgctctt ggtggatggg gattgggatc
     1561 acttgccaca gcgattggtg gctctgcggt attggcaatt ttaggaccag catttgtagg
     1621 agtaggctta atagcaattt tacttgtact tgcacttctt ggaatagtat tcgtaggtgt
     1681 aatcaaatta ttaataactt tgtacaaagc atactttggt attctcttgg gtgtagctat
     1741 aggacctctt cagattatga ttggtacaat acctggacag aatcatatga caatgaattg
     1801 gctcttggga atagtgagaa atgttttggt gtttccagta gtcttcttca tcgtgaatgc
     1861 tcctaatctt ttacttggaa gtggagatat tattttaaat ttcccagaga aattaactta
     1921 tgcagatcca aattcaacag ggatggatat aaatgcaact tctggattct taatcttcat
     1981 cttgcgtgtc tttgtaatat atcttgctgc acaagcacct aaatatatag atgcatttat
     2041 acctaatact tccagtaagg ctgcaggtga tgcaggtgct gctgcaaaga tgagtctttc
     2101 aaagattcct ttagttggcg gattatttaa atagctttta actataaatg cctaagccaa
     2161 ggaaagaaga aaatccagat gcacttggtc agatatttga cttcatattc aaagaggctg
     2221 aaaaaccacc tgataagaga agaccggtta aagtaactgg tatatctgga caaaatgaat
     2281 taacggatgc tcttgctgct gcattggaga aaccagggtt gtttatgagc gatcagatat
     2341 tagatgtttt taacgaagct ttggatctag aagttgcaag gataaaagca aatgagcaag
     2401 gatctgcgac agtaaagttc tcggctaata atctgatatc tatgcttgat gaccccaatg
     2461 cctttattga aaaaggtttt acaagtgcca aaacatttag gaagcaaatg cgagcacaat
     2521 atcttgggaa aactatgcaa cactttgttg ctgaaggatg ggcaaaaaaa tataatctag
     2581 atctagatgc acaagaagca attagaggct attttggagc aagacatctt gacaagaccc
     2641 tatcaaaaga ggaaagagca aaaagctatg atgcttctaa tgcgattggt gcttctgtag
     2701 aaggacgttt ttttagaggc tttccaggat caggggaact aaaccttaca tctgaaaaag
     2761 aacatttgac aaacagagct tttgaacttc ttgggagaca gtattttgga gcgtctaact
     2821 ggcaacaact ttcagatgac cagaagagat catttgaaaa agtacatata gattggagtg
     2881 caatgtctag tgcggataaa aagaaattca tggcggctaa caatggtatg gatgcagtag
     2941 aaaaatatat tacttcaaat tttaatccta ctactgcaaa cagtttattc aataattata
     3001 gaaatgctag aacaagtctt ggacttgcaa agaaagagga cacttttgat gtcgtgaata
     3061 gggatagata tttagatctc gaggttaaga acttagatga caggataacc caactaagac
     3121 agtacatgaa tactccaaat ctctcaaaca gagaggttac aaatttacag gtaggaattc
     3181 aaagactaga acaagcaaaa gtaatcgttg gaggtcaact acttgaccct gaaagtattc
     3241 atattgcaca gaaaaaaatt gattctgcag tagattcgtt tagactacaa atggatcaag
     3301 caagagcaca gggggatatg gattctgtga agttatatag gcaacagata aaagatttgc
     3361 gtactggaaa gagagaacta aatagtataa tgctttgggg aagagttggc caaatagagg
     3421 gatatataaa ctctttaaac ggtatttaca aagatggaaa ttttattaaa tcatatcttg
     3481 atggaacatt ttttgaccct aatagaaaca atcttttccg acctactgcg gatagggatg
     3541 tgaagtttag tttcggagag aaaaacggag agataggtat ccatgtagct gctcccacta
     3601 agaatgcttt attaaaaaca tacaacaaca cattaacaaa tctgtactat ctcaccccaa
     3661 aatccataat gaaaagcctt tttgtaaatg gggaaggctt tgtgtatttg acctatatga
     3721 aaaaactagg cttggaaggt agtttgaaag atatcctaca taataaaaaa ttcgaaatca
     3781 gtatagaaga tcttctttca actggaaatg cacaaaccta tttgaacaat attctttcag
     3841 gtacaaatcg tcaattaacg caacaagaga ttgaaaaata cacaaaagag ttaaaggcac
     3901 taatgagaag atctcgaaca gcaaaaatat tctcttttac acaaagaacc agagacaaaa
     3961 ttaagaaatt cttcgatgac aagattttca gagaaatgag acagaaaatt tacgacaaat
     4021 taatggctca aaaggcattt caagttgctg aggaagtact tggacagtgg cttgcgaagg
     4081 gtggcataca agtatttgta aaaggtttag taattggaat tacaaatgct gtgggaattg
     4141 taactactgg aggtcttgca aatttcgcaa taacaatagt aaccgccttg cttgtagatg
     4201 tcatgtatgg gattataaaa gttttagcaa tgatagttat ttttgcattt gttggaattt
     4261 ttgcaatggc atttatgctt ggtagtaagt caacatataa acatgcacta aatagctatg
     4321 catatactac agtagtacca ggagaagtag ttacaaaccc aaactttaga ggattgtatc
     4381 caatagatga tggagagatt ccaggagagc ttcatccatt tagtggagga tcacttcctg
     4441 atggagttca atgtttaatt ggcacgggtg gttttagatg ttctcagggt ccttttagta
     4501 gttattctca ctcaaaagtt gcagccattg atattactga tggtgtaaga accttctatg
     4561 ctcctacctt ttgtgatgga agtaattgtg tagttaaaac ggtagctgag gtcaattgca
     4621 caagatgtga tatcccgagt atgccaggat gtcatcagtt tccagcgggt ggcatggtaa
     4681 tatttgatgc aacatatggc tctacaacct acacattcaa actaatacat gtttacaaag
     4741 atgtgagcgt tgggcaaaag ctctcttctg gagaagctgt agcagatata atccaagctg
     4801 gcccagatca tgaaaggatt gagaagtgct cctcagggcc tcatctccat cttgaagtaa
     4861 aagcaaatgg tagttctgta aacccaatgg atgtaatgac tggaccaaca tcctctggtg
     4921 gttttgcgtg taatattagt gcatgtcctg taaattagta tgaacaaaaa gacaaaaaca
     4981 atattaataa tatccttctc tttactcttt gtaggagtat gtgtaggagt gtattttctt
     5041 ttaacaattc caccaaaaac atatccaaaa gatccaatcg agcaaacatc cttatatact
     5101 acatctcaag agattaactt ttatagcgaa gatttagaat ctaaagatta tcccttatat
     5161 ggagtatcaa gcgaagtaat gttaaaagaa gtcgaagcat ttgtatctac tattgctcct
     5221 caattaaaaa aaatttctaa tgacgaagga gcatattatg aatggggttt taaagaagat
     5281 tctgtgacat acaaccttac ccaaaatgcc ttaatgtttt catttgca
//
LOCUS       DAOQWP010000030         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_19447_length_4537_cov_5.599955, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000030 DAOQWP010000000
VERSION     DAOQWP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19447_length_4537_cov_5.599955"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKI16_03365"
     CDS             <1..784
                     /locus_tag="PKI16_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNR53486.1"
                     /translation="TKYLIATRSQDIVSLEEYSFKIVDLKEEEKNLAIQKAAVQVKRN
                     STEEEKLDLAKEAKNLETQKAEKDSLLAQSRANEAQLKATYEANIKKQNALDAAIIAY
                     INEHGDQARMSGRVVAGEWIGRMGNTGYSTGDHLHFSVRNSFVSGKVCQGNIPILSGY
                     LKQGPASWLVGWDDWKWPYMYPGTMRLPIAGPYVIMTQNYHSGYAIDLKSYKTDRTTN
                     YGAPIYAILPGTLYRGVDGHNGVYAYIKHDNGWVSCYLHIQE"
     gene            849..1661
                     /locus_tag="PKI16_03370"
     CDS             849..1661
                     /locus_tag="PKI16_03370"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNR53487.1"
                     /translation="MAKSTKKTTKKIVVKKAPAKKVRIVVKKPDISKVKDINFKPIGK
                     VIGLILIIVSAFALIDLAVQYLNNDYSVAVVDGTRISKSKWHKTLESAYGSSVAQQLI
                     DEQIIRAEAKKADVTVSKEEIDKQIADIETSLGGKEALDTALVANNITLEQLKEQVEL
                     DILSTKILTPQIEYTEDDVKSFFNQYSDVIFPDETAALEKGEKLDFDKYKEETEKVYI
                     QQQVQSKRSSWLTEVEGSHRIQDNSVSRPRYGFLTTTVNIINNLLESSKEAE"
     gene            complement(1709..2308)
                     /gene="raiA"
                     /locus_tag="PKI16_03375"
     CDS             complement(1709..2308)
                     /gene="raiA"
                     /locus_tag="PKI16_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNR53488.1"
                     /translation="MEEKDIQVSFVGMDPTEALKKYVLEKIMKKENLFTDATSIEVFF
                     KEFVHSKGVKHDFRVDINVKLPNTVVRVEESGSDMYANIDKGTDTLFRRLKRYNDKKE
                     YWEGKASWKILEAQEAIRALEEESGVEVDDYSDYIPKIAKRKIVEDMSPIEEGEAIER
                     MELSGYDQYLFRNKSNNKICMLYKRKQGGYGLVEPKDGI"
     gene            2394..2837
                     /locus_tag="PKI16_03380"
     CDS             2394..2837
                     /locus_tag="PKI16_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53489.1"
                     /translation="MENKQDTDFLNNPDEFLSISNTEQLIDEFGDMFDQTMLYKVPIY
                     LEKNQYGENTPEERFLATHTSLVTLRGQDIWDMDKDDQEFLEGVHKALTLMKVAIEGE
                     GIDFDRDIVVHGKNINVTYEPYTVDTSEYVIRELNERKELLQKKD"
     gene            2867..2938
                     /locus_tag="PKI16_03385"
     tRNA            2867..2938
                     /locus_tag="PKI16_03385"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2899..2901,aa:Asn,seq:gtt)
     gene            complement(3277..4380)
                     /locus_tag="PKI16_03390"
     CDS             complement(3277..4380)
                     /locus_tag="PKI16_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNR53490.1"
                     /translation="MTKPNGIENISSQEVGNVISLEDFRNSGESEELHYEIFNVPVER
                     IVLADNIRPFDNDFIDTLAMSIHKHGQLQACIGDIVCDGEGTFVRLIAGQHRFKSIVK
                     LNLEGEDIPVKISLANRELNPYEIIEIQITENLHNKMTPAQEAQVINGLWQKVRILKD
                     NGGEKLSIAEFARSVSRSPATVRDAIKYIEGLSPVVQRLVDEKILPYTVSLLLTEAEN
                     IIKGRDREKQYTLEDVYREQLYLAQKFVTMSFTYEKAKKYIEQLKQESNFSGPLFGDE
                     WKKIEFASHIIAIRDESDKKGRDAAGWFAKIIRMVQLLPDTNKVEFSDAIENAIFELG
                     MSRDEFEEKLRPLITEKQHIRLFGKGTKEELLV"
BASE COUNT         1523 a          832 c          718 g         1464 t
ORIGIN      
        1 aacaaaatat ttaattgcca caagaagtca ggatattgtt tcattagagg aatatagctt
       61 taaaatagtt gatttgaaag aagaagaaaa aaatttagca atacaaaaag ctgcagttca
      121 ggttaaaaga aatagtacag aagaagagaa attggattta gcaaaagaag ctaaaaatct
      181 tgaaacacaa aaagctgaga aagacagctt acttgcacaa tctagagcaa atgaagctca
      241 attaaaggcg acatatgaag caaatattaa aaaacagaac gctttggatg ctgcaattat
      301 tgcatatata aatgagcatg gagatcaggc acgaatgtct ggtcgtgttg tagctggaga
      361 atggattgga agaatgggaa atacaggtta cagtactgga gatcacttac acttttctgt
      421 tagaaattct tttgtctctg ggaaagtctg tcaaggcaat atccctatat taagtggata
      481 tctgaagcaa ggccctgctt catggttagt tgggtgggac gattggaaat ggccatatat
      541 gtatcctgga acaatgcgac ttccaatcgc tggaccgtat gtaattatga cccaaaatta
      601 ccacagtggt tatgctatag atctcaagtc atacaaaact gacagaacaa caaactatgg
      661 tgctccaata tatgcaattc tacctggaac actatatcga ggtgtggatg gtcataatgg
      721 agtatatgca tatattaaac acgacaatgg ctgggtaagc tgctatcttc acattcaaga
      781 gtaacacccc tttcacattt catcatattc tgctaaacta tagtttaata aattcataca
      841 gtcctttaat ggcaaaaagt actaagaaga ccactaagaa aatagttgtc aaaaaagccc
      901 cagcaaagaa agtccgtata gtagttaaaa aaccagatat ctccaaagta aaagatatta
      961 actttaaacc aataggaaaa gttattgggt taatactaat aattgtatct gcatttgcat
     1021 tgatagattt agcagtacaa tacctaaaca acgattacag tgtagctgta gtagatggta
     1081 caagaatatc taaatctaaa tggcacaaaa cattagaaag tgcatacggc tcttctgttg
     1141 cacaacagtt aatagatgaa cagataatca gagctgaagc taagaaagct gatgttactg
     1201 tctccaaaga agagatagac aaacagattg cagatattga aacaagtttg ggtggaaaag
     1261 aagcactaga tacagcattg gttgcaaaca atatcacatt ggaacaatta aaagaacagg
     1321 ttgaattaga tattctgtct acaaaaatat taactccaca gattgaatac acagaggatg
     1381 atgttaagag tttcttcaat caatacagcg atgtaatatt tcctgatgag acagcagctt
     1441 tagaaaaagg tgagaagctt gattttgaca aatataagga agaaacagag aaagtatata
     1501 tacaacagca agtacaaagt aaacgaagct cttggttaac agaggtagag ggaagtcaca
     1561 ggatacagga caactcagtt agcagaccaa ggtatgggtt tttaacaaca actgtaaata
     1621 taataaataa cctgcttgag agtagtaaag aggcagagta atctacattg gtatgtaaat
     1681 aagaaaggag gtctaagacc tccttttttt atattccgtc ctttggttcc acaagaccat
     1741 agcctccctg ttttcttttg tagagcatac atatcttgtt gttactttta ttcctaaaca
     1801 gatactgatc atatcctgac aattccattc tctcaatagc ctctccctct tctatggggg
     1861 acatatcttc tacaatcttt cttttagcaa tctttggtat gtaatctgaa taatcatcta
     1921 cctcaacacc agactcttcc tcgagtgctc taatggcttc ttgggcctcc aatatcttcc
     1981 atgatgcttt accttcccag tactctttct tgtcattgta tctttttaat cttctaaaca
     2041 atgtatctgt tcccttatct atatttgcat acatatcact tccactctcc tctaccctaa
     2101 caactgtatt tggtagtttt acattaatat ctaccctaaa atcatgcttt actcctttac
     2161 tatgtacaaa ttctttgaaa aatacctcta tggatgtagc atctgtaaaa aggttctcct
     2221 ttttcataat tttttctaat acatacttct taagtgcttc tgttggatcc atacctacaa
     2281 acgatacttg tatatctttc tcttccatac ctttttccta ataatttaga tatactaaat
     2341 aatatatttt ttaatatata tatggcaact atttaaatta aatatctcta attatggaga
     2401 acaaacaaga tacagatttc ctaaataatc cagatgaatt tctttcaata tctaatacag
     2461 aacaactaat agatgagttt ggcgatatgt ttgaccaaac aatgctttac aaagttccaa
     2521 tatatctaga aaagaaccaa tatggagaga ataccccaga ggaaagattt ttggctacac
     2581 atacatcatt ggttacatta agaggtcaag atatctggga tatggataaa gatgatcagg
     2641 agtttctaga aggtgtacac aaggctttaa ctttaatgaa agtagctata gagggagaag
     2701 ggatagactt tgatagagat attgttgtgc atggaaagaa tataaatgta acgtatgaac
     2761 cgtacacagt agatacctct gagtatgtaa taagagagtt aaatgaaagg aaagagttgt
     2821 tgcagaaaaa ggattagata tatataatag gtctggtgat agataatccg cgatagctca
     2881 acggtagagc aaacggctgt taaccgttcg gttacaggtt cgaatcctgt tcgcggagtt
     2941 ttttggtatt gttgattcac atttactaca tatacataca ttagaatcaa agtataagta
     3001 ggaatccact ataaaatatc ctagtagcaa tcatatacat catttgtgtt gttttattta
     3061 tgaattggac atcaaatagc agattcaagg ataattcctt acttatgcca ataatgatgc
     3121 tctcactttt caccatttct gctctggtca tgagacatgt attcttttat caactatttc
     3181 gactatactt agatgataaa aaggaagata cagttaaact gttcattcaa acttttgcaa
     3241 ttcttgctat tgttccattt agtattttac ttttatttat actaaaagtt cttccttagt
     3301 tcctttccca aataacctga tatgctgctt ctctgtaatc aaaggcctta acttctcttc
     3361 aaattcatct ctggacatac ctaattcaaa tattgcattt tcaattgcat cagaaaattc
     3421 taccttgttt gtatctggta ataactgaac cattctaata atctttgcaa accatcctgc
     3481 agcatcccta cctttcttgt cagattcatc cctaatggca atgatatgtg aagcaaattc
     3541 aatcttcttc cactcatcac caaacagtgg tccagaaaaa ttactctctt gctttaactg
     3601 ctcgatatat ttctttgctt tctcataggt aaaactcatt gtaacaaact tctgtgctag
     3661 gtatagttgc tctctataaa catcttccaa tgtatactgc ttctccctat ctcgtccctt
     3721 tatgatattt tccgcttctg ttaaaagcaa tgatacggta tatggaagta ttttttcatc
     3781 cactaatctc tgcacaacag gagacaaccc ctctatatat tttatcgcat cacgcactgt
     3841 agcaggacta cgactaacac tcctagcaaa ctctgcaata gaaagttttt ccccaccatt
     3901 gtccttcaat attctcactt tttgccatag accatttatt acctgagcct cctgtgcagg
     3961 agtcatttta ttatgtaagt tctctgttat ctgaatctct atgatctcgt atggattcag
     4021 ttctctgttc gccaaactta tctttactgg aatatcctct ccctctaaat ttaactttac
     4081 tattgattta aatctatgtt gaccagcaat taacctaaca aatgttcctt ctccatcaca
     4141 aactatatct cctatacatg cttgaagctg tccatgctta tgtatactca tagctagagt
     4201 atcaatgaaa tcattatcaa atggacgaat attatccgct aaaacgatcc tttccacagg
     4261 aacattaaat atttcataat gtagctcttc agattcacct gaatttctga aatcttccaa
     4321 ggatattaca ttcccaacct cttgacttga aatattctca ataccatttg gtttagtcat
     4381 attttctaga atacaagtta gcaatatatt atattaagaa gtttatatat tttcttttta
     4441 tcctttcgaa acccatagga ctattctaca tccatctcta taatcttgac aactttagca
     4501 ctcggtgata ccatttgcta gtagttaaat ttttata
//
LOCUS       DAOQWP010000031         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_21975_length_4142_cov_8.353805, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000031 DAOQWP010000000
VERSION     DAOQWP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21975_length_4142_cov_8.353805"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..972
                     /locus_tag="PKI16_03395"
     CDS             154..972
                     /locus_tag="PKI16_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HNR53491.1"
                     /translation="MKKILKAALMRQHPIPQNILDVEFKLFTKFTLKEFAYLAIGLGF
                     GGLMIYLTVKGMIPSLLGIPIFIFSSGAGIFLALVPINDQDADLFLKNYITAISSPTQ
                     RVWMNKKIRDERAKPLIKPTKEGTLVHKETPEKPKIIGATQIPEPTEIQEEQVQQPVK
                     SIPSSFLQINSENISAYQFSIKTLDSLPGNINIWLCTKSMKPIPNVIAYLKDKEGKVL
                     YANKTGPNGYFLTNKMWDSGIYTLEFQHPQYQFPKVQLILVDKNVKLPIKINNI"
     gene            974..1621
                     /locus_tag="PKI16_03400"
     CDS             974..1621
                     /locus_tag="PKI16_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53492.1"
                     /translation="MDNKARARAINSTQEHIEVKDIRDDLIITKSGVVSMVIQTSAIN
                     FDLLAEYEQDNKIYAFAGLLNSLNFKIQILIRTRRIDITNYVEYLRSKKTDSMSEGLK
                     KQLEIYTSFIQKLIVQNDVLDKSFYIVIPYNQGSYLPNTSIFKSKKDKEEQEEASRLQ
                     TEQLIEKAKIFLQPKRDHVLKQLGRMGLMGHQLTTKELLTEIYTIYNPEEEDDRE"
     gene            1608..3995
                     /locus_tag="PKI16_03405"
     CDS             1608..3995
                     /locus_tag="PKI16_03405"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNR53493.1"
                     /translation="MTENNNNYNRTFESKTVQQLKEKEVQTKFSTFKEEVKTENTEST
                     KKVEDAQRYNDPAEEKKKVNPLLKLIDKLSRKEKIEEEKQRYESTYTDVDTVAKDLTI
                     QDIIAPIDLEVDFNDLQIGDYYARTLFISGYPRFVGPNWLSPIVNFEHSLRISTFYYP
                     VDSKTILEKLKKKIGEMEASLYSQMEDRKVVDPSLKVALSDAQQLQDSIAEGTEKFFH
                     FAMYVTIYARNKELLEKYSRNVISTFAAMNVTARPATLQQEQGFISTQPIGVDKLYIT
                     RNMDTTSIATTFPFVSSELTMDHGIMYGINLHNKSLVIFDRFELENANTVVFAKAGAG
                     KSYFVKLEALRSLMLGTEIIIIDPEREYETLCTAVNGAYISFSQDKGNKMNPFELSGV
                     KDEGDDELRMKLLALHGFFKILFGGLNNIEESLLDRALILTYREKGITFDPSTQTKKP
                     PLLEDLYKVLRGMAETEAHDLSKRMEKYIIGSAAGVFNEESNFEINNPFTVFSVRDLQ
                     EELKPLAMYLMLDFIWTRIRKDIKRRLLIVDEAWYMMQSPDSASFMYSIAKRARKYYL
                     GLTTITQDVTDFLNNDMGKAIISNSSLQVLMKQSPSAVEKLQQVFNLSDGERNYLLNC
                     DRGQGLFFAGSNHVGIQILSSQAEHELVTSDPKDLERKKSMQGMTDTRGLEEMSQIYE
                     PPAQQSSSEEIGNVQRQDQIIRKAIQKRIEDETVIKDERERYKKELEEKIKEEEKLLN
                     PQKLKNQRSFLEELKQDTLSGQVIQSRQDIQKDKQHLTPQGLVESYQNNGQKQTV"
BASE COUNT         1626 a          738 c          707 g         1071 t
ORIGIN      
        1 aaactctttc cccaaatcct tttaatatca tcttttttta atgatgcacc catcaaagtc
       61 ctaaactttt ctagattttc cacactcttt tttatatctt ccattcttta ttatattaca
      121 tctaaaactt ttcccaatat tctggtaaac taaatgaaga aaatacttaa agctgcctta
      181 atgagacaac atccaatacc acaaaatata ttagatgtag agttcaaact ctttactaag
      241 tttactctaa aagaatttgc atacctagcc ataggacttg ggtttggtgg actaatgata
      301 tatcttactg ttaaaggcat gattccaagt cttctcggta ttccaatttt cattttttca
      361 tctggtgcag ggatcttcct tgcattagta ccaattaatg accaagatgc tgacctcttt
      421 cttaaaaatt acataactgc aatctcttcc cctactcaaa gagtatggat gaacaaaaag
      481 atcagagatg aaagagcaaa accattaatt aaaccaacaa aggaaggaac attggtacac
      541 aaagaaactc cagagaaacc gaagataatt ggagcaacac agataccaga accaactgaa
      601 atacaagagg agcaggtcca acaaccagtc aaaagtatcc catcttcttt tttacaaatt
      661 aatagtgaga atatttctgc ctaccaattt agtattaaga cactagattc tcttccagga
      721 aatataaata tatggttatg cacaaaaagt atgaaaccaa ttccaaatgt aatagcatat
      781 ttaaaagata aagaaggaaa agtgttgtat gctaacaaaa ccggaccaaa tggatacttc
      841 cttacaaata aaatgtggga ttctggtata tatacacttg aatttcaaca tccacaatac
      901 caatttccga aggtacagtt gattctagtg gataaaaatg taaaattacc aattaaaatt
      961 aataacatct gatgtggaca acaaggcaag agcaagagcc ataaactcaa cacaagagca
     1021 tatagaggtc aaagatatca gagatgactt aattattact aaaagtggtg tagtatcaat
     1081 ggtaatacaa acatctgcaa taaactttga ccttcttgcg gagtatgaac aagacaataa
     1141 aatatatgcc tttgctggac ttcttaattc tcttaatttc aaaatccaaa tactcattag
     1201 aacaagaaga atagatatca caaattacgt agaatatctt cgttcaaaga aaacagattc
     1261 catgagtgaa ggattaaaaa aacaattaga aatatatact tcgtttatac aaaaattaat
     1321 agtccaaaac gacgtcttag acaagtcttt ctacatagta attccttaca accaaggttc
     1381 ctacctacca aatacaagca tattcaaatc taagaaggat aaagaggagc aggaagaggc
     1441 atcaagatta caaacagaac agttgattga aaaggccaaa atatttcttc agccaaagag
     1501 agaccatgta cttaaacagc tcggaagaat gggccttatg ggccaccaat taaccaccaa
     1561 ggaactttta acagaaatat atacaatata taacccggaa gaagaagatg acagagaata
     1621 ataataacta taatagaaca ttcgagagta agactgtcca gcaattgaag gagaaggaag
     1681 tacaaacaaa attcagtaca tttaaagaag aggttaaaac agagaataca gaaagcacga
     1741 agaaggtaga agatgctcag aggtacaatg atcctgccga agagaaaaag aaagtaaatc
     1801 ctctattaaa attgatagat aaactctcta gaaaagaaaa aattgaggaa gaaaaacaaa
     1861 gatacgaaag tacatatacc gatgtggaca cagttgccaa agatttaaca atacaagaca
     1921 taatagctcc aattgattta gaggtagatt tcaatgattt acaaattgga gattattatg
     1981 ccagaacact tttcataagt ggttatccac gttttgtcgg tccaaactgg ctttctccaa
     2041 ttgttaattt tgagcactcc cttagaatta gtacattcta ctatcccgtg gattcgaaaa
     2101 cgattctgga aaaattaaag aaaaagatag gagaaatgga agcatctctg tactctcaga
     2161 tggaagatag gaaagttgta gatccttcgt taaaagtagc actttcagac gcccaacagc
     2221 ttcaagattc catagcagaa ggaacagaaa agttttttca ctttgctatg tacgtaacaa
     2281 tatatgccag aaacaaagaa ttacttgaga aatattctcg taatgtaatc tctacatttg
     2341 ccgcaatgaa tgtaactgca agaccggcta ccttgcaaca ggaacagggg ttcataagta
     2401 cacaaccaat tggagtagac aaactctaca taacaagaaa tatggatacc acatccattg
     2461 ctacaacatt cccatttgtt tcctcagagt taacaatgga ccatggaatc atgtatggaa
     2521 ttaacctgca taacaaaagc cttgtcatct ttgaccgttt tgagcttgaa aatgcaaata
     2581 ccgtagtatt tgctaaagcc ggagcaggaa aaagctactt cgtaaaactt gaagctttaa
     2641 gaagtcttat gctcggaaca gagataatca tcatagaccc tgaaagagaa tacgagacac
     2701 tctgtaccgc tgtaaatggc gcttacatat ctttctcgca ggacaaaggt aacaagatga
     2761 atccatttga actctcaggt gtaaaagatg aaggagatga tgaattaagg atgaaacttc
     2821 ttgcattaca tggatttttc aaaattctct ttggaggatt aaacaatatt gaagaatctc
     2881 tcttagatag agccctgatt cttacatata gagaaaaagg tataacattt gacccatcta
     2941 cacaaacaaa gaaacctccg ttgcttgaag atctctataa agtcctaagg ggaatggctg
     3001 aaaccgaagc acatgatctc tccaaaagaa tggaaaagta catcattgga agtgcagcag
     3061 gagtatttaa cgaagagagt aattttgaaa tcaacaatcc gttcactgtc tttagtgtaa
     3121 gagacttaca agaagaacta aaacccttag ctatgtacct tatgctcgac ttcatatgga
     3181 caagaattag aaaagatatt aaacgaagac tcctcatagt agacgaggct tggtatatga
     3241 tgcagagtcc tgattctgca agctttatgt actcaattgc aaaaagagct agaaaatact
     3301 acctaggact cactaccatt actcaagatg ttactgactt cttaaacaac gatatgggta
     3361 aagcaataat ttcaaactcc tcacttcagg tcctaatgaa acaaagtcca tctgctgtag
     3421 aaaaattaca gcaagtattt aatctttccg acggagagcg aaactacctt cttaactgtg
     3481 atcgtggaca aggactcttc ttcgcaggtt ccaaccatgt tggtatacag atactctcta
     3541 gccaagcaga acacgaactt gtaacttcag atcctaaaga tttggaaaga aagaagagta
     3601 tgcagggaat gacagatacc agaggtcttg aagaaatgtc tcaaatatat gagccaccag
     3661 cacaacaatc ctcttcagaa gagataggga acgttcaaag acaggaccag ataattcgaa
     3721 aagccataca gaaaagaata gaagatgaga cagttataaa agatgaaaga gaaagataca
     3781 aaaaagagtt ggaagaaaaa atcaaagaag aggagaaatt acttaatcca caaaagttaa
     3841 aaaaccaacg aagttttcta gaggagttaa aacaagatac tctttcaggg caggtaatac
     3901 aatctagaca ggatatacaa aaagataaac aacatcttac tccacaaggt ttagttgaaa
     3961 gctatcaaaa taatggacaa aaacaaacag tttaatattg ttaacgaaaa taattacctt
     4021 gtaacaaagg gagttgaagt ctcttcaatc tcccctaaca aacttacttc cgacttagaa
     4081 caattaaata aagcggaaga aaaaacaacc tacaaggcaa acattgcaaa gggggtaaaa
     4141 ga
//
LOCUS       DAOQWP010000032         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_31992_length_3194_cov_4.634278, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000032 DAOQWP010000000
VERSION     DAOQWP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31992_length_3194_cov_4.634278"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKI16_03410"
     CDS             complement(<1..418)
                     /locus_tag="PKI16_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNR53494.1"
                     /translation="MSKQTNSSQEERTKEYKKLKRLSVSTSNLLISIKSLAALLKASI
                     PLSETVKTISEQSSDPNLNKIYTYISQEIDKGSTLAEGLRLFPKVFPETVASVVEAGE
                     RGGSLENNLMFIAETIKKEWELKKKLKGAIIYPIIII"
     gene            complement(418..1833)
                     /locus_tag="PKI16_03415"
     CDS             complement(418..1833)
                     /locus_tag="PKI16_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNR53495.1"
                     /translation="MVELLKLTDDVIKKRINVSNFLDALILDALRKKASDIHIEAKEG
                     NTLLIRYRIDGVLRDIIPIGQEIKDSLLFIIKVRSKLRTDVHFAPQDGKILFTVKKEA
                     IDPIPEVTKEEEKKKEEPKEEKKEEKKDKKKKKETEEEVEPPVKNLLEKLPAQQAIEK
                     DYYHVDARISILPVTHGEKVVIRLLSQANRAYGLTDLGFEEEDLIKLEKAYKQPYGLF
                     LVTGPTGSGKTTTLYSILKILNTRDVNITTIEDPVEYSIEGVNHIQINPKGDLTFANG
                     LRSILRQDPNIIMVGEIRDKETARITVNSALTGHLVLSTVHANDAVSTIPRLIDMGIE
                     PFLVASTLNIVIAQRLARRLCPNCKKEYTPSRDVAHKEALKLRPDIQKHMANVTKVYR
                     ASGCKECNYTGYSGRIGIYEILPMTKALRDVIISSPNSDDILAAAKKGGFRLMVEDAV
                     KKLKDGYIDIKELTKVIAIKE"
     gene            complement(1889..2332)
                     /locus_tag="PKI16_03420"
     CDS             complement(1889..2332)
                     /locus_tag="PKI16_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNR53496.1"
                     /translation="MKGKKYQGFSLIEILVVVALIIILATITIVAINPAKNFRDTRNA
                     QRSADVMQILNAVTQYTSEEGHAIADLGTIPTCPATGPGTGVIGTGGINLSATLVDEY
                     LVAIPQDPSDGTDADTGYTICQTAGGRVQIDAPGAEASKVISVKR"
     gene            complement(2408..>3194)
                     /locus_tag="PKI16_03425"
     CDS             complement(2408..>3194)
                     /locus_tag="PKI16_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53497.1"
                     /translation="VQQPISEPPKKKSFVKPLLFIFIFLLLGAGIYYLIFEMNILETI
                     TGKNLDNSSTSEQDTQDTQDEEITEEETFTPFEGEYISTKLPEGWSVTEYLDGEGTTM
                     LPGETEYTGLTGLKIFNGDTEIFYMQAVSGLGFAGCPNYAKFADENPEYYAQILEDNE
                     VSGQELTVQDYTATEVEYEEFTWLGMEFRRIEKEYVYDEVANNEYFEPPCVPSLVAFD
                     NVKLYSDIDGYESSTFDYGAKDIATSQDLLVVDQILNDMELVK"
BASE COUNT          841 a          635 c          506 g         1212 t
ORIGIN      
        1 agattattat aatagggtag atgattgcgc cttttagctt cttttttaat tcccattctt
       61 tcttaattgt ttccgctata aacataaggt tgttttctaa agatccacct ctctcccccg
      121 cttccacgac agaagcaacg gtttctggga agacctttgg aaacaatctt aacccttctg
      181 ccaatgttga acccttatct atctcctgag atatatatgt gtatatttta ttaagattgg
      241 ggtcgctaga ctgttcagat attgtcttta cggtttcaga taatgggata ctagctttaa
      301 gtaaagcggc taaggattta attgatataa gcagattaga tgttgagaca gagagtcttt
      361 ttaacttttt atactctttt gttctttctt cttgtgaaga gtttgtttgt ttactcatta
      421 ctcttttatt gcaattactt ttgttaactc ttttatatca atataaccat cttttaattt
      481 cttaacggca tcctcaacca ttaatctaaa accacctttc tttgctgctg caaggatatc
      541 atcggagtta ggactactga tgattacatc tcttagtgct tttgtcatag gtaatatttc
      601 atatattcct attcttccag aataaccagt atagttacac tctttacatc ctgatgctct
      661 atatacctta gtaacattcg ccatgtgttt ttgtatatca ggtctaagct tgagagcttc
      721 tttatgggcg acatctctac tgggtgtata ctctttttta cagttaggac agagccttct
      781 tgcaagtctt tgagctataa cgatgttaag ggtagaagct accaaaaagg gttctattcc
      841 catatctatt aatctaggga ttgtacttac tgcgtcattc gcatgtactg ttgagagtac
      901 taagtgtcct gttagagctg agttaactgt aattcttgct gtttctttat ctctaatctc
      961 cccaaccata attatattag ggtcttgcct taagatagat ctaagtccgt ttgcaaaggt
     1021 taaatcaccc tttgggttaa tttgtatatg atttactccc tctatactat actcaacagg
     1081 atcttctatc gttgtaatgt ttacatccct agtgttaagt atcttaagaa tggagtagag
     1141 tgttgtagtt tttccacttc ctgttggacc agtaaccaag aatagtccat acggttgttt
     1201 atacgctttc tctagtttaa ttaaatcctc ttcttcaaat cctagatctg ttaaaccgta
     1261 agctctattt gcttgagata acaaccttat taccaccttc tctccgtgtg ttacgggcag
     1321 gatagatatt cttgcatcta catggtagta atctttctct atggcctgct gcgctggtag
     1381 tttctctaat agatttttta caggtggttc tacttcttct tctgtttctt tcttcttctt
     1441 tttatctttt ttctcttctt tcttctcctc tttcggttct tctttctttt tttcttcctc
     1501 ttttgttact tcggggatgg ggtctattgc ttctttttta acagtaaaaa gaattttccc
     1561 atcctgtgga gcaaagtgaa catctgttcg aagtttagat ctaactttaa ttataaagag
     1621 caagctatct ttgatttctt gccctattgg tataatatct cttaagacac catcgattct
     1681 gtatctaata agaagagtat taccctcttt tgcctcaatg tgaatatcag atgccttctt
     1741 tcttagcgca tccaatatta atgcatctaa aaagttagat acatttatcc tttttttaat
     1801 tacatcgtct gtaagtttta aaagctcaac catggcagta cggatagctt ttggctatct
     1861 gatattcact ttatatttaa atcagcagtt atctctttac acttattact ttgctagctt
     1921 ctgcacctgg agcatcaatt tgaactctcc ccccagcagt ttgacaaatt gtataaccag
     1981 tatcagcatc tgttccatca gaagggtctt gaggaattgc aactaggtat tcatctacta
     2041 atgttgcaga taaattgatt ccaccagtac ctataacccc tgttcctgga cctgtcgctg
     2101 gacatgttgg aatagtacct aagtctgcaa ttgcgtgtcc ctcctcagaa gtatactgtg
     2161 ttacagcatt taggatttgc ataacatctg cacttctttg agcatttctt gtgtctctaa
     2221 agttctttgc agggttaatt gcaacgattg taatagtagc caagattatg ataagtgcta
     2281 ctactacaag gatctcaata agtgagaatc cttgatattt ttttcctttc ataaagaaaa
     2341 aatattaaat ttatatacat attaaatata catttgtaca ttattcgtgt caatcaaaac
     2401 attcctctta tttcaccaac tccatatcat taagtatctg gtcaactaca agaaggtctt
     2461 gtgaagttgc tatatctttc gctccataat caaaggtgga agattcatat ccatctatat
     2521 ctgaatatag ctttacatta tcaaaagcta ctaaagatgg tacacaagga ggttcaaagt
     2581 attcattgtt tgcaacctca tcatatacat actctttctc tatccttcta aattccattc
     2641 caagccaagt aaattcttcg tactctacct ctgttgcagt gtaatcttga acggttaatt
     2701 cttgtccact tacctcatta tcttcaagta tttgagcata atattctggg ttctcgtcgg
     2761 caaatttagc atagtttgga caccctgcaa aacctaaacc agatactgcc tgcatgtaaa
     2821 aaatctctgt atctccattg aaaatcttta atccggtaag accagtatac tcagtttctc
     2881 ctggtagcat agtagttcct tctccatcta agtactctgt tacgctccat ccctctggta
     2941 gctttgttga gatatactct ccttcaaatg gagtgaatgt ctcttcctct gttatctctt
     3001 catcttgtgt atcttgtgtg tcctgttcac tcgtactact gttgtctaaa ttctttccag
     3061 taatagtttc tagtatattc atctcaaata ttaggtagta tattccggca cctagaagta
     3121 agaagataaa tatgaagagt aggggtttta caaaagattt cttttttggg ggttctgaaa
     3181 taggctgttg aact
//
LOCUS       DAOQWP010000033         2939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_36100_length_2939_cov_5.505548, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000033 DAOQWP010000000
VERSION     DAOQWP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36100_length_2939_cov_5.505548"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..329
                     /locus_tag="PKI16_03430"
     CDS             6..329
                     /locus_tag="PKI16_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53498.1"
                     /translation="MQHKQQASGEWFKKPFLEQMANIGSEVYRAINWRNKGNEEYAQM
                     AFIKSLELFDLSKNSKFTYPQYKELLRMREIWVDFFKYDNEYNSTQDSINKYFMYTAI
                     GFKAS"
     gene            468..1040
                     /locus_tag="PKI16_03435"
     CDS             468..1040
                     /locus_tag="PKI16_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNR53499.1"
                     /translation="MDQIPGSEIENNEVDKYLSSGSQTQITPEIEKITSEISGTVLEK
                     TQKILEIGPTLVERKDFDERVFRKRSGAQIIQDKYITGCTDAALVFIVLARASGIPTK
                     YIETIDVEWLKKGGSSIGGHVYAQVYDEAQDKWVWVDPMRRNVGNSPGEDRVIFDEGL
                     DSWDLGITDYNSLRSEFEIFRKQWKSKPSA"
     gene            1298..>2939
                     /locus_tag="PKI16_03440"
     CDS             1298..>2939
                     /locus_tag="PKI16_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53500.1"
                     /translation="MKKTFSKLFELKLEKRQIIIISIVIVALIVISLAVYFVLRGKNL
                     PTFINNNAQKTFQEGFADDSEEALTTLAQPYDTEDTEPTYDLYAEVGTPNEDSTYDTV
                     QTSSNFNPDSEECSNSEEGSCIDLGREKTVMPNHSTVNLIVWGSYATEWSDEGLPGAR
                     VGLKAYAVLPDVSMASIYSDSKVQFNFYDKETRIVQMEGEVYYRVKPQEEGHVFTVQA
                     GDRLIELNDAEAYVQIVHDVDAETARYEGWEKLEKELGLEDYDYYDDSGEFDSAKYDE
                     YERLSEEYRKEIENSKGDIYAIYVYITSGNAKITSRSDRNTVLSELNGDNPFTILKYR
                     EPEENTRVYFDAEQEEIDEKKENLATGYESTEFMANLTFSMSNYGMGSFGGVSKEDLV
                     NMLQININAIEPQARKTAAWFKNDMENFLSDWNEFVAKTSCKAGWYQATSETCCPDGY
                     IWDSSRQLCTKTTYTYYCNDGYTLGSDNMCHKSGSAGESIKPTVGNTPAFTSGDCPPA
                     GDIVITWCYPEDGNTKSGYVFCGYNIVNNKEKYCQDTVL"
BASE COUNT         1068 a          392 c          625 g          854 t
ORIGIN      
        1 aaaatattca gcataaacaa caagcttctg gagaatggtt taagaaacca tttctagaac
       61 agatggctaa tattggtagt gaggtatata gagcaattaa ttggagaaac aagggtaatg
      121 aagaatatgc acaaatggct tttattaaat ctttagagtt atttgatttg tcaaaaaatt
      181 ctaaatttac atatccccaa tacaaggaat tgctaagaat gagagagata tgggtagatt
      241 tttttaaata tgataatgag tataactcta cacaagatag tataaataaa tattttatgt
      301 atactgctat tggttttaaa gcatcctaag aaatttaaca taggtcttaa taaaacatgt
      361 tgaaaatggc gggacataca cgtctggacc ctatctaaaa tgagaatgag acaaaagaga
      421 cagctaaaaa agaagaggga gtgtatcatt tctgatataa tttacatatg gatcaaatac
      481 caggttctga aattgaaaat aatgaagtag ataaatactt atcttctggt tcacagactc
      541 aaataacacc agaaatcgag aaaatcacct ctgaaataag tggaacagtt cttgagaaaa
      601 cacaaaagat cttagagata ggacctactt tagtagagag gaaagatttt gatgaaagag
      661 tatttagaaa aagatctgga gctcaaataa tccaagataa atatataaca ggatgtacgg
      721 atgctgcttt agtattcata gtattggcta gggcgagtgg aataccaact aaatatattg
      781 aaactataga tgttgaatgg ctcaaaaaag ggggaagttc tattggaggt catgtatatg
      841 ctcaagtata tgatgaagcg caagataaat gggtctgggt agatccaatg cgtagaaatg
      901 ttggtaattc tcctggggaa gatagagtta tattcgatga gggacttgat tcttgggatt
      961 taggaataac agactataat agcttaagga gtgaattcga aatatttagg aagcaatgga
     1021 agtcaaagcc aagtgcctaa aagcttctac cttcgaaccg aggactcaaa aaaatcatcc
     1081 tttttcggtc tttttcatcc accaggcaat aaatttgaac actcctgttt ttgagggtaa
     1141 acgtatcaag cagcaaaggg atctttggat atagtggacg ggtctaattg gaacaactca
     1201 aatatggcgg gaaatacagg tcaggaacaa tatcttctaa ctttgacatc actaataacg
     1261 agtgataaat ttattaataa tctgtattct tcactaaatg aaaaaaactt tctcaaaact
     1321 ttttgaatta aaactcgaaa agaggcaaat aataattatt agtattgtta ttgtagctct
     1381 aattgttatc tctttagcag tgtatttcgt attgagaggg aaaaatctcc caacatttat
     1441 taataacaat gcacaaaaga cttttcaaga aggatttgcg gatgattctg aagaagcatt
     1501 aactactctt gctcaacctt atgatactga agatactgaa cctacttatg atctgtatgc
     1561 agaagtaggt actcctaatg aggactctac ttacgatact gtacagacaa gttctaactt
     1621 taatccagat tctgaagaat gtagtaattc tgaagaggga agttgtatag atttaggaag
     1681 agaaaaaacg gttatgccta accattccac agtcaatctt attgtctggg gatcatatgc
     1741 tacagaatgg agcgatgaag gtcttccagg tgcaagggta ggtcttaagg cctatgctgt
     1801 cttacctgat gtatctatgg caagtatata cagtgatagt aaggttcaat tcaatttcta
     1861 tgacaaagaa acaaggattg tgcagatgga aggagaggta tattaccgtg taaagcctca
     1921 ggaagagggg catgtattta cagtacaggc aggagatagg ttgatagagt tgaacgatgc
     1981 tgaagcatat gttcaaattg tacatgatgt agatgctgaa acagctaggt atgaaggatg
     2041 ggagaagtta gagaaggagc taggccttga agattatgat tattatgatg attctggaga
     2101 attcgattcg gccaagtatg atgaatacga aagattatca gaagaatatc gtaaagagat
     2161 tgaaaatagc aagggagata tatatgcaat atatgtatat ataacatctg gtaatgcaaa
     2221 gattacatca agatcagata gaaatactgt tctttctgag ctgaatggag ataatccatt
     2281 tacaatcctt aaatatagag agcctgagga gaatactagg gtatattttg atgctgagca
     2341 ggaggagata gatgaaaaga aggagaatct agcgactggt tatgaatcaa cagaatttat
     2401 ggctaatctt actttttcta tgtccaatta tggaatggga agttttggag gagtatcaaa
     2461 ggaagatctt gtaaatatgt tacaaataaa tattaatgcc attgaaccac aagctagaaa
     2521 gacggctgca tggtttaaga atgatatgga gaatttcctt agtgattgga atgaatttgt
     2581 agctaaaacg tcatgtaaag caggatggta tcaagcaaca tctgagacat gttgtccaga
     2641 tggatatata tgggattcat ctaggcaatt atgtacaaag actacttata catattactg
     2701 taatgatggg tatactcttg gatcagataa tatgtgtcat aaatcaggat cggctggtga
     2761 atctataaaa ccgacagtag gaaatactcc cgcttttacc tcaggagatt gtcctccagc
     2821 aggtgatata gtcataactt ggtgttatcc agaagacggt aatacaaaat ccggatatgt
     2881 attttgtggg tataatattg ttaataataa agaaaaatat tgtcaagata ctgtgttag
//
LOCUS       DAOQWP010000034         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_44879_length_2552_cov_5.954746, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000034 DAOQWP010000000
VERSION     DAOQWP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44879_length_2552_cov_5.954746"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..>2552)
                     /locus_tag="PKI16_03445"
     CDS             complement(121..>2552)
                     /locus_tag="PKI16_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53501.1"
                     /translation="ISNTIVKEFNDALDVKIAQVDFFGGRGSLKLTTNNLGSWLRDPG
                     GQIQKAMDNAKADRKAARARMIGESIDDFLTTAWAHKYGNLEAKEIARANASANEKAE
                     SYKIARAVGQRMVPEGEKDFMIDRSVNLLGQKTFGNRWFSMNEQERLEFSRRLAAGNS
                     RQGVISYLAQRYGQTEAQNFDSALIKQPGDRINIYDPDLYKGLENNYLTEKIYSLRDA
                     PEGSEEEKQRKIYERTKMLINIKTRQEIDALKGKLKEGNLSADQKREIERAINDAEIV
                     LREVGGVHNLATGIGRWEGYLNSRKMLGGLSRTNLVGSILNGDFFDPRKNKRSPVESK
                     KIEEMPGVEIYVFKKDPKGNRVMNAYNEMGEALYYATPRSLFRTFFYNGELFARGLYK
                     NNEQLKGLLNGAGAGALGDDVVKSIFSLKGEELDKEINSILKKIKIQIGEGKLSQKDF
                     ERIEKLIGQSKKFKNLTRAFSMPARTKKAIEDQLKKVFDKPLRKFRASIVRTLLKNKT
                     IKEWIIKNHGGKLLGEFVKNGGLQNLMKPLVSAIAGALGIALTPLASIAITIGVNIAM
                     DLAGKIAKVFAQMMLLAIVGLIAGIVVLGGSIGSWKKWNKKTYSYNYVVPDTVNQCPA
                     YGGQNYNIPPPDGGEGWVPGPGLPDDWEGAKGVYEIFKRARDYVSREYGTVNTELVLL
                     DCNSGSDPTGMCAAIDWAWCYSASSIYCKVDKLTSASPQYVFALSVHELIHQLQRGCT
                     TDIREWGADYLSSNGGGYVFSTPSGCKRATEINTSSCSRQEAVDAALCRNTNTDCFRQ
                     ISGQILGRFCH"
BASE COUNT          738 a          584 c          320 g          910 t
ORIGIN      
        1 ttaaataacc atcttgagta acttcttcct tagagagaag taagagtaca agcagaatag
       61 taagaagaat aactccgact attatatata ttttgtattt attaaaaaat tctttcatct
      121 ttaatgacag aatctcccta atatttgacc agatatctgc ctaaaacaat ctgtatttgt
      181 atttctacac aatgcggcat caacagcttc ctgccgagaa catgatgatg tattaatctc
      241 agtagctctt ttacatccac ttggtgtact aaaaacatat ccacctccat tagaagatag
      301 ataatctgct ccccattctc taatatctgt agtacagcct ctttgtaatt gatgtattag
      361 ttcatgtaca gaaagcgcaa agacatactg tggtgaagca ctcgttagct tatccacctt
      421 acaatatatt gatgatgcac tataacacca agcccagtct attgctgcac acattcctgt
      481 tggatcactc ccactgttac aatcaagcag tacaagctca gtatttacag taccatattc
      541 tcttgataca taatctcttg ctcttttaaa aatttcataa actccttttg ccccttccca
      601 atcatctggt agtcctggcc cagggaccca gccctcacca ccatcagggg gtggaatatt
      661 ataattttgt cctccatatg caggacactg attaacagtg tctggtacaa cataattata
      721 tgagtatgtc ttcttattcc atttcttcca tgatccaata ctaccaccta atacaacaat
      781 tcctgcaata agaccaacta ttgcaagcaa catcatctgt gcaaaaacct ttgcaatttt
      841 ccctgccaaa tccatagcaa tatttacacc aattgttata gcaatagatg ctaatggagt
      901 aagagcaatt ccaagagctc cagcaattgc agaaacaagt ggtttcatca aattttgaag
      961 tccaccattt ttgacaaatt ctcctaataa ctttcctcca tgatttttaa ttatccattc
     1021 ttttatggtt ttattcttta gtaatgtcct tacaatactc gccctaaact tcctaagtgg
     1081 cttatcaaat actttcttaa gttgatcttc tatcgccttc tttgttctag caggcatact
     1141 aaatgccctt gttaaattct taaacttttt tgattgtcct attagtttct caatcctttc
     1201 aaaatctttt tgcgaaagct ttccctctcc aatttgaatt tttatttttt taagaatgct
     1261 attaatctct ttatcaagct cttctccttt taaagaaaat atacttttaa caacatcatc
     1321 acctaaagct ccagctccag caccatttaa aagtcctttt aactgctcat tgtttttata
     1381 caaacctctt gcaaataact ctccattata aaagaatgtt ctaaaaagtg atcttggtgt
     1441 tgcatagtaa agagcttctc ccatttcgtt atatgcattc ataactctgt ttccttttgg
     1501 atctttctta aaaacataga tttcaacacc aggcatttct tcaatttttt tgctttcaac
     1561 tggagagcgt ttatttttac gaggatcaaa gaaatctcca ttaagaatag agccaacaag
     1621 gttagttcgt gataagcctc caagcatctt tcgtgaatta agatatcctt cccatcttcc
     1681 aatccctgta gccaaattat gtacaccacc aacttctcta agtactattt ctgcatcatt
     1741 tattgcacgc tcaatttctc ttttttgatc agcagagaga ttcccctcct taagcttacc
     1801 ctttaaagca tcaatttctt gtcttgtttt aatattgata agcatcttag ttctttcata
     1861 tatctttctt tgcttttctt cctcggaacc ttctggtgca tcccgtaaag aatatatttt
     1921 ctccgtaaga taattattct caagcccttt atacaaatct ggatcatata tgttaatcct
     1981 atcgcctggc tgttttatta aagcactatc aaaattctga gcctctgtct gaccatatct
     2041 ttgagctaga taagagatta ctccttgtct actgtttcct gctgcaagcc ttcttgagaa
     2101 ttctaatctt tcctgttcat tcatagaaaa ccaacgattt ccaaatgttt tctgccctaa
     2161 caaattaaca ctcctatcta tcataaaatc tttttctcct tctggtacca ttctttgccc
     2221 tactgctctt gcaatcttgt atgattctgc tttttcattt gcagaagcat ttgcccgggc
     2281 tatctcttta gcttctaaat tcccatattt atgagcccat gcagtagtta agaaatcatc
     2341 tatagactca ccgatcatac gagcccttgc agcttttcta tctgcctttg cattatccat
     2401 tgctttttga atttgacctc caggatctcg tagccaactt cctaaattat ttgtagtcag
     2461 tttcaaactc cctctgccac caaaaaaatc aacctgagca atcttaacat ctaaagcatc
     2521 attaaattcc ttaacaattg tatttgaaat aa
//
LOCUS       DAOQWP010000035         2436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_48319_length_2436_cov_5.443511, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000035 DAOQWP010000000
VERSION     DAOQWP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48319_length_2436_cov_5.443511"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1243)
                     /locus_tag="PKI16_03450"
     CDS             complement(113..1243)
                     /locus_tag="PKI16_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNR53502.1"
                     /translation="MNEDSMGDILALENKLVTSNLPLDLKEETMKNLLRLKSMIKRGV
                     YGSEFDTMIKYVNWITKIPFGAVSKDNLDISNAKRLMDETHYGMDSIKELILDYLAAM
                     QLENMQRNRIPTQVATDMSYRPQPTVALKPNSKAPVFLFIGLQGVGKTSIAESIANAM
                     GRKFARISLGAMGDVRTLRGTPKYSIDSEPGQIVKSLIRCGTMNPVILLDEIEKASGN
                     AGLLNDVMAALLEILDPEQNGNFVDHYVDYPIDLSNVFFICTANNLGGLSAALLDRVE
                     IIRFTSYSDEEKEIIAKRYILPKVLQALQISGEYLRIDDNVWPMIIRPVGFDAGIRQL
                     ERNIATIARRAARIIIEGKQIPVVITQENLKQFLNIDQGPLS"
     gene            1406..>2436
                     /locus_tag="PKI16_03455"
     CDS             1406..>2436
                     /locus_tag="PKI16_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53503.1"
                     /translation="MKKLSIYLFLVAFAFLPLKALAAVDITLEDKSTTTQKSVDILVN
                     TGADTTEKIGFQIQASEGVTISSISDDSTVCSSFSYTNVSNLLTITCTVTEAKALNGN
                     VATILFAPNSDDYAFTVSQTTGLDLGTLTLGTVTDVTNVAEEVTTAAPVVTTTTTETE
                     TTKSFQDMLTDYLPYILIGGATILLISILGILLSKKKDGKPEEVVTQVEETKEETPAV
                     SESRPTDIDNISNNLYTPQVKEETIQEKLEEQPVQLQSTEAPSAATESNDLADLMAKD
                     SSSVTEEVPTPDFNAMPVETPAPTPEFNAMPIETPTPVVEETTPAPDFNAMPVETPTP
                     MVEETTPVMP"
BASE COUNT          786 a          497 c          417 g          736 t
ORIGIN      
        1 ataaagcgga agaaaaaaca acctacaagg caaacattgc aaagggggta aaagatccgc
       61 tggtatatag agcaatgagt gctttgagat tggcaaatcc atttaaggga tattagctaa
      121 gaggaccctg atctatattc aagaattgtt ttaaattctc ctgtgttatt acaactggta
      181 tttgcttacc ctcgatgatt atccttgctg ctcttctagc tatagtggca atgttccttt
      241 ctaactgtct aataccagca tcaaaaccca cgggtctaat tatcattggc catacattat
      301 catcaattct aagatattca cctgatattt gaagtgcttg tagaacctta ggaaggatat
      361 atctcttcgc aattatttcc ttctcctcat cactgtagct tgtaaatcta ataatctcta
      421 ccctatccaa aagtgctgca gataatcctc caaggttgtt tgcagtacag atgaagaata
      481 cattagataa atctattggg taatctacat aatgatctac aaaattacca ttttgttctg
      541 gatctagtat ctcaagaagt gctgccataa catcattaag gagacctgca tttccgcttg
      601 ccttttctat ttcatcgaga agtataactg gattcatagt cccacaccta attagagatt
      661 taacaatctg accaggttca gaatctattg aatattttgg ggtccctcta agagtcctta
      721 catcacccat tgctcccaga gaaattctcg caaattttct ccccattgca ttcgcaattg
      781 attccgcaat ggaggttttg cctacaccct gtaaacctat gaacaaaaat actggggctt
      841 tggaatttgg cttaagggca acggttggtt gaggtctgta agacatatcc gtagcaacct
      901 gtgtgggaat tctgtttctt tgcatattct caagctgcat agctgcaaga taatcaagaa
      961 tcaattcttt gatgctgtcc ataccgtagt gtgtttcatc catcagtctc tttgcatttg
     1021 atatatccaa attgtctttt gaaacagcac caaatgggat ttttgtaatc cagtttacat
     1081 atttaatcat agtatcgaat tcagaaccgt atactccacg ttttatcatg gatttaagcc
     1141 ttagaagatt cttcatggtt tcttccttca aatccaaagg aagattggaa gtaacaagct
     1201 tgttttctaa tgcaagaata tctcccattg agtcttcatt catactcccc gtattatttt
     1261 gtaagaaatt tgtatccgaa taattcatag taggcagatt ttactgttat cctagtgtat
     1321 atttcattac tgattaagtc aaatattttg acattatata tgacaaatga tagccttaga
     1381 atagataaaa ttatactgcc ttaaaatgaa aaaactttct atctatttgt ttctcgttgc
     1441 attcgctttt cttccactaa aagctttagc agcagtcgac attactcttg aggataaatc
     1501 tactactaca caaaaaagtg tagatatact tgtaaatacg ggtgcggata ccacagagaa
     1561 aataggattt cagatccaag cttcagaagg tgtaaccatc tcctccattt cagatgacag
     1621 tactgtatgt agttcgtttt cctacaccaa cgtgtcaaac ttactaacaa taacctgtac
     1681 cgttacagag gcaaaggcct taaatggaaa tgttgctaca atactttttg cacctaactc
     1741 agatgattat gcatttacag tatctcaaac tactggtttg gatttgggta ctttaacatt
     1801 gggaacagtt acagatgtaa caaacgtagc agaagaggta acaactgcag caccagtagt
     1861 tacaacaact acaacagaga cagaaaccac aaaaagcttc caagatatgc ttacagacta
     1921 tcttccatac atcttaattg gtggagcaac tattttacta attagcattc ttggaatatt
     1981 acttagtaag aagaaagatg gaaaaccaga agaagtagta actcaagttg aagaaacaaa
     2041 agaagagacc ccagcagtat ctgagagtcg tcctacagat atagataata tatctaacaa
     2101 tctatacaca ccccaggtta aggaagaaac aatacaggag aaattagagg aacagcctgt
     2161 acagctacag agcacagagg ctccttcagc agcaactgaa tccaatgatt tagcagattt
     2221 aatggcaaaa gatagctctt cagttacaga agaagtacct actcctgact ttaatgccat
     2281 gccagttgaa actcctgcac ctacaccaga gttcaacgct atgccaattg aaactccgac
     2341 accagtagtt gaggaaacaa cacctgctcc tgactttaac gctatgcccg ttgaaactcc
     2401 aacccctatg gttgaagaga caactcctgt tatgcc
//
LOCUS       DAOQWP010000036         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_53170_length_2289_cov_5.794987, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000036 DAOQWP010000000
VERSION     DAOQWP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53170_length_2289_cov_5.794987"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="PKI16_03460"
     CDS             <1..1784
                     /locus_tag="PKI16_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53504.1"
                     /translation="EERIFPPTETLPEQPLGSTVPSFTIATEKKPQPDINPLHEEALR
                     QNRERNVGFRGPLSDKGAQALKDIDTIADDLSGKNFKQLTDLMGPEEARRYEDALREK
                     EENTPPTPEPTPKPTPEPTPEPAPEPTPKPTEEDRLAELNKTLEETNNTLKDIDLNIQ
                     TLLQQGIENFSREQMLEYIKLIQEQSGLITKTYEIQQEKNRIAFENAIKMLQGTVPIP
                     PTPEPAPEPPPQPEPEPTPEPAPEPPPQPIVTVSDADRIKELEDQLRELRGENTPEQQ
                     SAALGRELAELEEKLASVGLTDAEKIKYFDILKAKENIDKQINQVEQENKKKKERKEK
                     IIKIAAGIVGAGVALTTPALGVAAVIGITFGGRTLGRVAQKQSEKLRSQSNSIKYESR
                     RGKTLIELEEMDRKQKRKEFWANRLGEASALLIGGSTGYAIGTLFEGIVGKDFHIGGG
                     KAPETPIGTTSNNTPTATSESGSLGGNTSSAQASAPNSTMPDATTIGTSPENWLTGET
                     FNASELGWNYNEMGWLGDRVHLTELGGQNGIMQGKFFQELSRLVPKDRLVGQASGNIV
                     NQFLRQAYGGLDPAVAAQKAAEALLGS"
     gene            1816..2226
                     /locus_tag="PKI16_03465"
     CDS             1816..2226
                     /locus_tag="PKI16_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53505.1"
                     /translation="MKDEIIQKIKEAGVPEQEVETFYNLLSEQILDLLFQDLAEKQTD
                     EELTVIENRIKDAKSTEHLEAIIQEIALTVYGENAAQEINNMYLDLIDDFKKNVEDAK
                     ALIERANQGDKDAIELLNKAMNTDMYKSIMGLKE"
BASE COUNT          961 a          382 c          426 g          520 t
ORIGIN      
        1 ttgaagaaag aatctttcct cctacagaaa ctttaccaga gcaacctctt ggtagtactg
       61 tacctagttt taccatagca acagaaaaga aaccccaacc tgacataaat cctttacatg
      121 aggaagcatt aagacaaaat agagaaagaa atgtaggatt tagaggacct cttagtgata
      181 aaggagctca agctttaaaa gatattgata ccattgcaga tgatttaagt ggcaaaaatt
      241 ttaaacaatt aacagaccta atgggacctg aggaggctag aagatatgaa gatgccctta
      301 gagaaaaaga agagaataca cctccaacac ctgaaccaac acccaaacca actcccgagc
      361 caactcctga accagcacca gaaccaactc ctaaaccaac tgaagaggat agattagcag
      421 aattaaataa aactcttgaa gaaacaaata acacattaaa agacatagat ttaaatatcc
      481 aaacattact acaacaaggt attgagaatt tctcaagaga acaaatgctt gaatacatta
      541 agctaataca agaacaatca ggattaataa caaaaaccta tgaaatacag caagaaaaaa
      601 acagaatagc atttgaaaat gctattaaaa tgctacaagg aacagtaccc attcctccta
      661 cacctgaacc agctcctgaa ccacctccac aaccagaacc agaaccaact cctgaaccag
      721 caccagaacc acctccacaa ccaattgtaa cagtatctga tgcagataga attaaggaac
      781 ttgaagacca acttagagaa ttaagaggag aaaatacacc tgagcaacaa tctgcagcgt
      841 taggaagaga attagcagaa ctagaagaaa aattagcatc agtaggttta actgatgctg
      901 aaaaaataaa atattttgat attttaaaag caaaagagaa tatagataaa cagataaatc
      961 aggtagaaca agagaataag aagaagaaag aaagaaagga gaaaataata aaaatagcag
     1021 ctggaatagt tggagcagga gttgcattaa ccacaccagc attaggagta gctgctgtaa
     1081 ttggaattac atttggcggt agaacattag gtagagtagc acaaaaacaa tcagagaaat
     1141 taaggtctca atctaattca ataaaatatg aatcaagaag aggaaaaaca cttatagaat
     1201 tagaagaaat ggatagaaaa caaaagagaa aagaattctg ggctaacaga ttaggagaag
     1261 ctagtgcact attaataggt ggctcaacag gatatgccat tggaactctt tttgaaggaa
     1321 tagttggaaa agattttcat ataggaggag gtaaagctcc tgagacacca attggtacaa
     1381 caagtaacaa tactccaaca gcaacatcag aaagtggatc tttaggtggg aacacatcat
     1441 ctgcacaagc atcggcgcct aatagtacaa tgcctgatgc tactaccatt ggtacaagtc
     1501 cagagaattg gttaacagga gaaactttta atgcatcgga acttggctgg aattataatg
     1561 agatgggatg gttaggagac agagttcacc ttacagaact tggaggtcaa aacggtatca
     1621 tgcaaggaaa attctttcaa gagttatcta gattagtacc aaaagacaga ttagtaggtc
     1681 aagctagtgg aaatatagta aatcaattct taagacaggc atacggagga cttgatcctg
     1741 ctgttgcagc acaaaaagca gcagaagcct tgcttggtag ctaataaata aattatattt
     1801 taaataaaaa gagaaatgaa agatgaaatt attcaaaaaa taaaggaagc tggagtacct
     1861 gagcaagaag ttgaaacatt ttacaatcta ctttcagaac aaatcttaga cttactcttc
     1921 caagacttag cagaaaagca aacagatgaa gaattaactg ttatagaaaa cagaattaaa
     1981 gatgcaaaat caacagaaca tcttgaagca atcattcaag agattgctct cacagtatat
     2041 ggagaaaatg ctgcacaaga aataaacaat atgtatttag acctaattga cgattttaaa
     2101 aagaatgtcg aagatgcaaa agctttaata gaaagagcaa accaaggaga taaagatgca
     2161 attgagctac ttaacaaagc aatgaatact gatatgtata aaagtataat gggacttaaa
     2221 gaatagttct ctattgcaaa ggatgactct ttctaacagt ttcttctgca agtttttcac
     2281 tagcatgta
//
LOCUS       DAOQWP010000037         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_56854_length_2194_cov_6.723703, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000037 DAOQWP010000000
VERSION     DAOQWP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56854_length_2194_cov_6.723703"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKI16_03470"
     CDS             complement(<1..523)
                     /locus_tag="PKI16_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53506.1"
                     /translation="MSIKQILDIEKDIKDLNIQGATNVAIATVEGMKIFIEESAITDK
                     ELFYTELKRVGEKLANARPNEPLAENAVKYVSHMFKQHFSDLHSLNDMKQQLDDYCDE
                     YLFKIADCKRAIVDLGVPYVKHFENILTHCHSSTAVEVIKGIAKGKKSFDAVCTETRP
                     LFQGRITAKSLLDA"
     gene            complement(525..1952)
                     /locus_tag="PKI16_03475"
     CDS             complement(525..1952)
                     /locus_tag="PKI16_03475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR53507.1"
                     /translation="MKLKFCGATRTVTGSCFYIDTGEFKFLVDCGAFQGGDELERLNY
                     EPFPFDPASLDAVFLTHAHYDHCGRIPILVKQGFRGRIIGTQPTRDLARIVLLDAAKL
                     QKEEYERWQARSKEMPKGTNFSGEGMAYEQRKPLFTEEDVENMFQFFDVYPYGSSITV
                     SSDVEFRMRDAGHILGAAIFEIWIKTPEGRERKLVFSGDLGQPGARIVRDPDMIREAD
                     YVICEATYGNRLHKSKDETVLEFLSILKQAQEEKSNVLIPSFAIERTQEILYEINLFI
                     ENKILNNLEVYLDSPMASKATEIFKQYPTFYDEDARRLLEKGDDPFNFPGLVAVESAE
                     ESKRLINKQGVVIIAGSGMCTGGRIVHHIKNNIEKENTHMVFVGYQVEGTLGRKIVDR
                     EKQIRVMGQDMEVRAKIHTLGGFSAHGDQRDLRYWLRGFGHSPKKIFIVHADEEIGIG
                     FGSNIRTELGVEVDIPKLNEEFELK"
     gene            complement(1989..>2194)
                     /locus_tag="PKI16_03480"
     CDS             complement(1989..>2194)
                     /locus_tag="PKI16_03480"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HNR53508.1"
                     /translation="KRISEVRAKDMALKVTDLAVNGNDLEKIGVKPGPEMGKVLRSLL
                     DMVLEDPLINTKEKLLEAARNML"
BASE COUNT          645 a          500 c          263 g          786 t
ORIGIN      
        1 ctgcatctaa aagactttta gcagttattc tcccttgaaa aagtggtctt gtttcagtac
       61 atactgcatc aaatgatttt tttccttttg caattccttt tatgacctca acggcagtag
      121 atgaatgaca gtgtgtcaaa atattctcaa aatgcttaac atacgggaca cctaaatcaa
      181 caatagctcg tttacagtct gcaattttaa aaaggtactc atcacaatag tcatctaact
      241 gttgtttcat atcattaagt gaatgtaaat cagaaaagtg ctgtttaaac atatgagaga
      301 catatttaac agcattctct gctaatggtt cgttaggtct tgcattcgca agcttctccc
      361 ctaccctttt tagctctgtg taaaacagtt ccttatctgt aattgctgat tcttctataa
      421 aaatcttcat tccttcaaca gtagcaatag ctacattcgt agcgccttga atgttcaaat
      481 cttttatatc tttttctata tctaatatct gcttaatact catattactt taattcaaac
      541 tcttcattta atttaggaat atcaacttca acacctaact ctgtcctaat attactacca
      601 aaccctattc caatctcttc atccgcatgt actataaata tcttcttagg tgaatgccca
      661 aaccctctaa gccaatatct taaatctctc tgatctccat gggcggagaa tcccccaaga
      721 gtatgaattt ttgctcttac ctccatatct tgccccatta cacgaatctg tttttctcta
      781 tcaacaattt ttctcccaag tgtaccttca acctgatacc ctacaaatac catatgtgta
      841 ttttcctttt caatattatt cttaatatga tgtacaattc ttcctcctgt acacatacca
      901 cttcctgcaa taattacaac cccttgttta ttaataagtc tctttgattc ttctgcagat
      961 tcaactgcaa ccaatcctgg aaagttaaaa gggtcatctc ctttttcaag aagtcttctt
     1021 gcatcttcat catagaaggt aggatactgt ttaaatattt ctgtagcctt tgaagccatc
     1081 ggactatcaa gatatacttc taaattattt agtattttat tctcaataaa tagatttatc
     1141 tcatacaata tctcttgtgt tctttctata gcaaaagaag gaattaaaac attagacttc
     1201 tcctcttgtg cctgttttaa aatagaaaga aactctaata cagtttcgtc tttactttta
     1261 tgcaacctgt taccatatgt tgcctcacat atcacataat ccgcttctct aatcatatct
     1321 ggatctctta caattcttgc ccctggctgt cctaaatctc ctgaaaacac tagctttctt
     1381 tctcttcctt ctggcgtttt aatccaaatc tcaaatattg cagccccaag tatatgtcct
     1441 gcatccctca ttctaaattc tacatcactg cttacagtta tagatgaacc atatggatat
     1501 acatcaaaaa attggaacat gttttctaca tcctcttctg taaataatgg ctttctttgt
     1561 tcatatgcca ttccttctcc agagaaattc gttccctttg gcatctcctt acttcttgcc
     1621 tgccatctct catactcttc tttttgtaat ttcgctgcat ctaaaagaac tattcttgct
     1681 aaatctctag ttggctgtgt tccaattatt cttcccctaa atccttgctt tacaagaata
     1741 ggaattcttc cgcagtgatc ataatgtgca tgtgtaagaa atactgcatc taagcttgca
     1801 gggtcaaaag gaaaaggttc ataattaagc ctttcaagct catcaccccc ttggaatgca
     1861 ccacaatcaa ctaagaactt aaactcacct gtatcaatgt aaaaacatga acctgtaact
     1921 gttctagtgg ctccacaaaa ttttagtttc atacagggca gtaaacaaat taactactat
     1981 ataatatatt acaacatatt cctggctgct tccaatagct tctcttttgt attaattaaa
     2041 ggatcttcta aaaccatatc taaaagagac ctcaaaacct ttcccatttc aggaccaggt
     2101 ttaacaccta ttttttctaa atcatttcca ttgactgcca aatcagttac cttaagagcc
     2161 atatcttttg ctcttacttc agatatacgc tttt
//
LOCUS       DAOQWP010000038         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_60990_length_2099_cov_8.922701, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000038 DAOQWP010000000
VERSION     DAOQWP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60990_length_2099_cov_8.922701"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..438
                     /locus_tag="PKI16_03485"
     CDS             154..438
                     /locus_tag="PKI16_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53509.1"
                     /translation="MEKKREIKEITEDFGKEVAKHQELSLWWSNCKMFMNKGDIAELF
                     RLLDILVLNANYVRNRKNKDYGSEYYNVVVHVLKMANKPVNELLSKLNKE"
     gene            441..1010
                     /locus_tag="PKI16_03490"
     CDS             441..1010
                     /locus_tag="PKI16_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53510.1"
                     /translation="MKKKIIEKLELPYDDGKGNFSSGSGEIHILRKINEIIDYLNQSQ
                     KQPEGETIQNYNPEKGWDRYKKSKIEVKEFNLVPKVKSEKQEEWKWGIQEALEKDFEK
                     LYVKEYTTGWKEQRYLRNIEIGIMGFDNLERFISQLLSERTREVLEDLQKEFSEYGVP
                     DDVRYTHPLFYINNIIGEKLSKLSKKKNE"
     gene            1272..1817
                     /locus_tag="PKI16_03495"
     CDS             1272..1817
                     /locus_tag="PKI16_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53511.1"
                     /translation="MDKIEKLEITRQPGKELNHLDKNLASLVDKINEIVDVLNQSQKQ
                     PKGETIRNYNPEKRWDKYKKSKIEVREFDLVPKVQPEKQEGWRELSVNTRRILKTLGY
                     YFSDGNEHYSCPVCGIDVVRLDQLLSERTREAKIEVLEKVLEKKTNYEIEREEHGYRV
                     TLEYVLADDIKKEIKLLKERK"
     gene            1814..>2099
                     /locus_tag="PKI16_03500"
     CDS             1814..>2099
                     /locus_tag="PKI16_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53512.1"
                     /translation="MKIIKAKLANYQDRVDGTVSLKLDSLLEVPDSDIAEIRGMRGNI
                     AIAVITDVVDVLDADINTKDIMEHLPEDPFLDTRITPSQQQRRDLFVIQEK"
BASE COUNT          860 a          239 c          482 g          518 t
ORIGIN      
        1 aaagaacttt ggggaataga gccagacgat gattctccag cagtatgtga tgactgttgg
       61 gagaagatac acccatcaag gcatgtagac ctatataaac aagttaaaaa aatagttaaa
      121 aagacaggta aattaaattt ttaaacaata acaatggaaa agaaaagaga gataaaagag
      181 attacagaag actttggaaa agaggttgct aaacatcagg aactttcttt gtggtggagc
      241 aattgcaaaa tgtttatgaa taagggggac attgcagagt tgtttaggtt actagatatt
      301 cttgtattaa atgctaatta tgtgagaaat aggaaaaata aggattatgg ttctgaatat
      361 tacaatgttg tagttcatgt attaaagatg gctaacaagc ctgtgaatga gttactatct
      421 aaattaaaca aggaatagag atgaagaaga agataataga gaaattagaa ttaccatatg
      481 atgatggaaa aggcaacttt agttcaggtt ctggagaaat acatatactt agaaagatta
      541 atgagataat agattacctt aaccaatccc agaagcaacc agaaggagaa acaatacaga
      601 attataatcc agaaaaaggg tgggataggt ataagaagag taaaattgaa gtcaaagagt
      661 ttaaccttgt accaaaagtt aaatcagaga agcaagagga gtggaaatgg ggtatacaag
      721 aggcgttgga aaaagatttt gagaaattgt atgtaaagga atacacaaca ggttggaaag
      781 agcaaagata tctccgaaac attgagattg gtattatggg ttttgataac ttagaaaggt
      841 ttatatctca actactatcc gagaggacaa gggaagtatt agaagattta caaaaagagt
      901 ttagtgagta tggagtgcca gatgatgttc gctacactca cccactattc tatataaaca
      961 atataatagg agaaaagtta tctaaattat caaagaagaa aaatgagtag gatagacaaa
     1021 atgtttagta taaggggttc tggggagatt gggtaccggg cagacgtgga atttgccaat
     1081 atgtgtatgg ctggtcagct aaagttgtat ttatacgagt tgcaacgaga acaatttaat
     1141 atacacagga aaaatgaaga ttggttaaat cttttagatt tatacagaac acggcgaacg
     1201 caaaccgatt ttgttaaggt aaaaggtttt gaagaagaga agtcatctaa attattaaag
     1261 gagaaagaat aatggataag atagagaagt tagagataac aagacaacca ggaaaggaat
     1321 tgaaccacct tgataaaaat ctagcgagtc ttgtagataa aataaacgag atagtagatg
     1381 tacttaacca gtcccagaag caaccaaagg gggagacaat acgaaactac aacccagaaa
     1441 agaggtggga caaatacaag aaaagtaaaa tagaagttag agagtttgac cttgtgccaa
     1501 aagttcaacc agagaagcaa gaggggtgga gagagttaag tgtgaatact aggagaatat
     1561 taaaaacact tggatactat ttttcagatg ggaatgaaca ttatagttgc cctgtgtgtg
     1621 gaatagatgt ggtaaggtta gaccaactac tatccgagag aacaagagaa gcaaagatag
     1681 aggtattaga gaaagttctt gagaagaaaa ctaattacga aatagaaagg gaggaacatg
     1741 gatatagggt tacattagaa tatgttttag cagatgatat taagaaagaa attaaactat
     1801 taaaggagag aaaatgaaga ttattaaagc aaaattagca aactatcagg atagggtaga
     1861 tggaacagta tctcttaaac tagattctct tttagaagtc cctgatagtg atatagcaga
     1921 gataaggggt atgaggggta acatagcaat agcggttatt actgatgttg tagatgtgct
     1981 agacgcagat ataaacacta aggacataat ggaacactta ccagaagatc cattcctaga
     2041 taccagaata acacctagtc aacagcaaag aagagatttg tttgtaatac aggaaaaga
//
LOCUS       DAOQWP010000039         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_63233_length_2053_cov_6.076076, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000039 DAOQWP010000000
VERSION     DAOQWP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63233_length_2053_cov_6.076076"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..1102)
                     /locus_tag="PKI16_03505"
     CDS             complement(392..1102)
                     /locus_tag="PKI16_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNR53513.1"
                     /translation="MPYKDLQKRKKYKRRYYIKNREKILEQAKEYGMRTKEVRSKKAK
                     EYYEKNKERIKERVRGWRKEHKEEHLKSVKEWSKNNPEKRKAIVKRYYEKKKREDPEY
                     FNQKARKEYNSDIEKSREKLREYRQKNPIKTRLWKYNRRVRSGFTTVDKEHMTKRFED
                     AVLQRLKEQDYKCLYCGKDIEDDFSIDHIIPLSRGGTNEPDNIDLVCKDCNTRKSTRT
                     KSEYLSVLDNQPDSSLTL"
     gene            complement(1137..>2053)
                     /locus_tag="PKI16_03510"
     CDS             complement(1137..>2053)
                     /locus_tag="PKI16_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53514.1"
                     /translation="TSEDSFESLRDIQIRKMGEGNVSIEYPDRYITELSFEELNEEMI
                     RRSRLRQKVENKEYNTTIEIKTDRPIGLVWWADQHVGGQFVDYERLKWEADEIKANPY
                     LRVALGGDFSDSFVWFPAAFDDVANLNEQNLYLYKLVEYVGWDKILFCVIGNHPKFAR
                     RTGLDGYNEMREKIPVFDGVGTVDLVINGITYTGGVIHKARGASYIDPNFGGKRFLRE
                     NDGYDFVMTAHSHEAGSQTINRKDAKGEREVVLLSGKTFKETDDFMDTEGFKRKTGVG
                     LGSNGIIFNHQKKSMLPVSSFSKMLDYI"
BASE COUNT          511 a          472 c          250 g          820 t
ORIGIN      
        1 attgtgatat gggtcttatg tatccaacgc acctagaata tatctctgtt ctctgtctgt
       61 ttaccattgt caaataattg ttaagttaat tgtaaagggg gtgtgaggga aacattgtcg
      121 caaaccctca ccgttttact tgtttttagc gaacccccgt tgctgttatt cttacccctt
      181 tattaccagc agccctgctg aacttatcga gtgttgcagg ggtacagggc ttttgtttcc
      241 ataacatacg gaaaaccgta tgctttagaa cctactatat attttaccat taatctgcac
      301 agtcttaccg tacaaattac gcctattttg tacacccaac tccttaaatt aagcctaatt
      361 tatgtattac actcctcaaa ttaagcctaa tttacagagt taaagagcta tcaggttgat
      421 tatctaaaac actcaaatac tctgattttg ttctagttga tttccttgta ttacaatctt
      481 tacacaccaa atctatattg tctggctcgt ttgttcctcc ccttgataaa ggtattatat
      541 gatctatact aaaatcgtcc tcaatatcct ttccacaata taaacactta taatcttgct
      601 cttttaatct ctgcaatact gcatcttcaa atctttttgt catatgttct ttatctacgg
      661 tagtaaaacc acttctaact ctcctgttat atttccaaag tcttgtttta attggattct
      721 tttgtctata ctctcttaat ttctctctgg atttctcaat atcgctatta tattcttttc
      781 tagctttttg attaaaatat tctgggtctt ctcttttctt tttctcgtaa taccttttta
      841 caatagcttt tctcttttct ggattatttt tgctccattc ttttacactt tttaggtgtt
      901 cttccttgtg ttcttttctc caccctctaa ctctttcttt tattctttct ttgtttttct
      961 cataatactc ttttgctttc ttgctacgga cttcttttgt tctcatgcca tattctttag
     1021 cttgctctaa tattttctct ctattcttta tatagtatct tctcttgtat ttttttctct
     1081 tttgtagatc tttgtacggc atactagatt atatcatact gttttctatt ttccccttat
     1141 atatagtcta acattttact aaaagagctt actggtagca tgcttttctt ctgatgatta
     1201 aagataattc cattgctacc aagtccgact cctgtttttc tcttaaaccc ctccgtgtcc
     1261 ataaaatcgt ctgtttcctt aaaagtcttt cctgataaaa gaacaacctc tctctctcct
     1321 ttggcatctt ttctgtttat agtttgcgac ccagcttcgt gcgaatgagc agtcattaca
     1381 aaatcatatc catcattttc tcttaggaat cttttacctc caaagttagg gtctatataa
     1441 gaggctcctc tcgctttatg aataactcct cctgtatacg ttataccgtt tatgactaaa
     1501 tctacagttc ccacgccgtc aaagactggt attttctctc tcatttcgtt gtacccatca
     1561 agcccagttc ttctagcaaa cttagggtga tttccaataa cacaaaaaag tatcttatcc
     1621 caaccaacat actccactag tttgtataaa tagagatttt gttcattcaa atttgccaca
     1681 tcatcaaaag cagctgggaa ccatacaaac gagtcactga agtctccacc aagtgcaact
     1741 cttaaatatg ggtttgcttt aatctcatct gcttcccact ttaacctctc ataatctaca
     1801 aattgacctc caacatgctg atctgcccac cataccaagc ctataggtct gtctgtcttt
     1861 atctcaatgg ttgtgttata ttctttattc tcaacctttt gtcttaacct gcttcttctg
     1921 atcatttcct cattaagctc ttcaaagctc aattctgtaa tatacctatc agggtattct
     1981 atacttacat ttccttcccc catttttcta atctggatat ctcttaaaga ctcaaaacta
     2041 tcctctgaag ttg
//
LOCUS       DAOQWP010000040         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_74400_length_1860_cov_6.794460, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000040 DAOQWP010000000
VERSION     DAOQWP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74400_length_1860_cov_6.794460"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKI16_03515"
     CDS             complement(<1..190)
                     /locus_tag="PKI16_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53515.1"
                     /translation="MSIKQILDIEKDIKDLNIQGATNVAIATVEGMKVFIEESAITDK
                     ELFYTELKRVGEKLANARP"
     gene            complement(192..1619)
                     /locus_tag="PKI16_03520"
     CDS             complement(192..1619)
                     /locus_tag="PKI16_03520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNR53516.1"
                     /translation="MKLKFCGATRTVTGSCFYIDTGEFKFLVDCGAFQGGDELERLNY
                     EPFPFDPASLDAVFLTHAHYDHCGRIPLLVKQGFKGRIIGTQPTRDLARIVLLDSAKL
                     QQEEYERWQARSKDKRVGINFAEEGMAYEQRKPLFTGDDVENIFQFFDVYPYGSSVSV
                     SNDVEFRMRDAGHILGAAIFEIWIKTPEGRERKLVFSGDLGQPGARIVRDPDLIREAD
                     YVICEATYGNRLHKSKDETVLEFLSILKQAQEEKANVLIPSFAIERTQEILYEINLFI
                     ENKILNNLEVYLDSPMASKATEIFKQYPTFYDEDARRLLEKGDDPFNFPGLIAVESAQ
                     ESKRLINKQGVVIIAGSGMCTGGRIVHHIKNNIEKESTHIVFVGYQVEGTLGRKIVDR
                     ERQIRVMGQEMEVKAQIHTLGGFSAHGDQRDLRYWLRGFGHSPKKIFIVHADEEIGIG
                     FGSNIRTELGVEVDIPKLNEEFELK"
     gene            complement(1655..>1860)
                     /locus_tag="PKI16_03525"
     CDS             complement(1655..>1860)
                     /locus_tag="PKI16_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HNR53517.1"
                     /translation="KRISEVRAKDMALKVTDLAVNGNDLEKIGVKPGPEMGKILKSLL
                     DMVLEDPLMNTKERLLEAAKNML"
BASE COUNT          536 a          441 c          220 g          663 t
ORIGIN      
        1 taggtcttgc attcgcaagc ttctccccta ccctttttag ctctgtgtaa aacagctcct
       61 tatctgtaat tgcagattct tctataaaaa ctttcattcc ttcaacagta gcaatagcta
      121 cattcgtagc tccttgaatg ttcaaatctt ttatatcttt ttctatatct aatatctgct
      181 taatgctcat attactttaa ttcaaactcc tcatttaatt taggaatatc aacttcaaca
      241 cctagctctg tcctaatatt acttccaaac cctatcccaa tctcttcatc tgcatgaact
      301 ataaatatct tcttaggaga gtgtccaaac cctcttaacc aatatcttaa gtctctctgg
      361 tctccatgtg cagaaaagcc tcctaatgta tgaatctgag ctttaacctc catctcttgt
      421 cccattacac gaatctgcct ttctctatca actatttttc tcccaagtgt accttctacc
      481 tgatatccta caaacactat atgtgtactt tccttttcaa tattattctt aatatgatgt
      541 acaattcttc ctcctgtaca cattccgctt cccgcaataa ttacaacccc ttgtttgtta
      601 ataagtctct ttgattcttg tgcagattca actgcaatta atccagggaa gttaaaaggg
      661 tcgtcaccct tttcaagaag tcttctggca tcttcatcat aaaaggtagg atactgctta
      721 aatatctctg tagcctttga agccattgga ctatcaagat atacttctaa attatttagt
      781 attttattct caataaaaag gttaatttca taaagtattt cctgtgttct ttcaattgca
      841 aaagaaggaa ttaaaacatt agctttttct tcttgtgcct gttttaaaat agaaagaaac
      901 tccaatacag tttcatcttt acttttgtgt agtctattcc catatgttgc ttcgcatatt
      961 acataatctg cctctctaat caaatctgga tctcttacaa ttcttgcccc tggttgtcct
     1021 aaatctcctg aaaacacaag ctttctttct cttccttctg gtgttttaat ccaaatctca
     1081 aatattgcag ccccaaggat atgccctgca tctctcattc taaactcaac atcattacta
     1141 acactaacag acgaaccata tggatacaca tcaaagaatt gaaaaatgtt ctctacatca
     1201 tctcctgtaa ataatggctt tctttgctca tatgccatcc cctcctcagc aaaattaatg
     1261 cctacccttt tatctttact tcttgcctgc catctttcat attcttcctg ctgtaacttt
     1321 gctgaatcta aaagaactat tcttgctaaa tctctagttg gctgtgttcc aattattctt
     1381 cccttaaacc cctgctttac aagaagagga attctcccac agtgatcata atgggcatgt
     1441 gtgagaaata ctgcatctaa gcttgcaggg tcaaagggaa aaggctcata attgagtctc
     1501 tcaagctcat cacctccttg aaatgcacca caatcaacta agaacttaaa ctctcctgtg
     1561 tcaatgtaaa aacacgaacc tgtaactgtt ctagttgctc cacaaaattt tagtttcata
     1621 cagggcagta aacaaattaa ctactgttaa tatattacaa catattcttt gctgcttcca
     1681 atagcctctc ttttgtattc attaaaggat cttctaaaac catatctaaa agagacttca
     1741 aaatcttccc catttcagga ccaggtttaa cacctatttt ttctaaatca ttcccattta
     1801 ctgctaaatc agttacctta agagccatat cttttgctct tacttcagat atacgctttt
//
LOCUS       DAOQWP010000041         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Dojkabacteria bacterium isolate
            AMR_MDS_1059 NODE_88202_length_1676_cov_7.239358, whole genome
            shotgun sequence.
ACCESSION   DAOQWP010000041 DAOQWP010000000
VERSION     DAOQWP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32852402
            Sequence Read Archive: SRR4236648
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Dojkabacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Dojkabacteria bacterium
            Bacteria; Candidatus Dojkabacteria.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 12:09:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 708
            CDSs (total)                      :: 659
            Genes (coding)                    :: 656
            CDSs (with protein)               :: 656
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Dojkabacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88202_length_1676_cov_7.239358"
                     /isolate="AMR_MDS_1059"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2099670"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.84 N 89 W"
                     /collection_date="2014-11-24"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKI16_03530"
     CDS             complement(<1..245)
                     /locus_tag="PKI16_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNR53518.1"
                     /translation="MLFKEIKHEFEIDGRKCSFETGKLALRSESSILARMGDTVITVN
                     VNCAPQKQEFDFFPLTVEYIEKFYASGKINGSRFVKR"
     gene            complement(327..590)
                     /gene="rpsO"
                     /locus_tag="PKI16_03535"
     CDS             complement(327..590)
                     /gene="rpsO"
                     /locus_tag="PKI16_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNR53519.1"
                     /translation="MSLEKTEKQNVVNKYALHEGDTGSPEVQIALLTLQIEKLSEHLK
                     GHKKDNHSRKGLLQMVGKRRRLIEYLKSNEPDRYTELAKKLKI"
     gene            complement(697..>1676)
                     /locus_tag="PKI16_03540"
     CDS             complement(697..>1676)
                     /locus_tag="PKI16_03540"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNR53520.1"
                     /translation="AGVVGRAFVLESVKDRSKGNMSSLALIQGHICPGDWFGYKVGRE
                     FKIEKVKGIITEENENVCILNCGCGGKVLGVSNLLELGTEVFILEKNDIKILQNLYKQ
                     EAKAEAESEFTIEDFFVENKVDPSKMLNVIIKSSSEGSLEAIRKAVLKIEEDGYSVNI
                     LLDGVGNISMSDVERAQISKAIVLGFEVGIEAGVEDLAKKNKVLVRTYDIIYKLIDEI
                     SEAVTMMSLPESTEEEIGNATIKTIFVLTNGTKVLGSRVNEGIMKRDCKVDIVRKDEI
                     IAEGKIKSLRINKDIVNEVKSGSDCGIQLEKDVDVKEGDEVHCYRIVRQ"
BASE COUNT          468 a          346 c          213 g          649 t
ORIGIN      
        1 tctcttttta caaatctaga tccatttatc tttcctgatg catagaattt ctctatgtac
       61 tctactgtaa gaggaaaaaa atcaaattct tgtttttgag gagcacagtt aacattgact
      121 gttattactg tatctcccat tcttgccaat attgaggact cagatctcaa agctaatttt
      181 cctgtttcaa aggaacactt tctaccatct atctcaaatt catgttttat ttctttaaaa
      241 agcatgtata ttaattaaaa aattatgtaa tactaaagta taactatggt tatagtataa
      301 attgaaagtt tttacaaaga aagaggctat atttttaact tcttcgctaa ttcggtatat
      361 ctatcaggtt cgttactttt gaggtactcg attaatcttc ttctttttcc taccatttga
      421 agtaatcctt ttctagagtg attatctttc ttatgccctt tgagatgttc acttagtttc
      481 tctatctgta aggtgagaag tgcaatctga acctcaggtg aaccagtatc accctcgtgg
      541 agggcgtact tatttacaac attttgtttt tctgtttttt ctaatgacat actggttaaa
      601 tatagagtat cattatcttt ttctatttac aatagggatt cccaaattgg aaacaaggtt
      661 tattgtataa ttttataggt catctgtcaa gaagtactat tgtctaacaa tcctgtagca
      721 gtgcacttca tcgccttctt ttacatcaac atccttttct aattgtattc cacagtctga
      781 cccacttttt acttcgttaa caatatcttt gttaattcta agagatttaa tctttccttc
      841 tgcaattatt tcatcttttc ttacaatgtc taccttgcaa tctcttttca ttattccttc
      901 gtttacccta ctaccaagca cttttgttcc gtttgtaaga acaaagattg tcttaattgt
      961 agcatttcct atttcttctt cagtactctc tggaagagac atcatagtga cagcctcaga
     1021 aatttcatct attagtttgt agattatgtc ataggttctt accaagactt tgttcttctt
     1081 tgcaagatct tcaacaccag cttcgatacc gacttcaaat cccaatacaa ttgctttgga
     1141 gatttgagct cgctcaacat cactcattga aatatttcct acaccatcta ggagaatatt
     1201 tacactgtat ccatcttctt caatcttaag gactgctttt cttattgctt caagtgatcc
     1261 ttcagaagaa cttttaatta ttacatttag cattttactt gggtctactt tattttcgac
     1321 aaagaaatct tcaatcgtaa attcactctc tgcctctgcc tttgcttcct gtttataaag
     1381 attttgaagt attttaatat cgtttttctc aagaataaaa acttctgtac caagttccaa
     1441 aagatttgat actcctaaaa ctttgcctcc acagccacag ttaagtatac agacattctc
     1501 gttttcctcc gttattatcc cttttacctt ctctatcttg aactctctcc ctaccttgta
     1561 tccaaaccaa tctcctggac atatatgacc ctgtattagt gctaatgagg acatatttcc
     1621 tttagaacga tctttaacag attcaagaac aaaagctctt ccaacaactc ctgccg
//
